LOCUS       DAOPOA010000001        16101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_4321_length_16101_cov_3.440608, whole genome shotgun sequence.
ACCESSION   DAOPOA010000001 DAOPOA010000000
VERSION     DAOPOA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 16101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16101
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4321_length_16101_cov_3.440608"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PKJ17_00005"
     CDS             complement(<1..856)
                     /locus_tag="PKJ17_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOD74373.1"
                     /translation="MQRQSGWRSKSKEDIKEIYSFAEGYKAFLDEAKTEREAVRSIIR
                     TLEAEGFGTDPSGERVFAVNRGKEVMAFVRGSGDISEGLNIVVAHIDAPRLDLKQNPL
                     YEDIDLALLRTHYYGGIKKYQWVAVPLAIHGVVVRSDGETVDICIGEKEDDPVFLIDD
                     LLPHLSRKTQDEKKLGDAIDGEKLVVLFGSLPLTGAEKEPVKQYVLKILGDAYGMTED
                     DLISAEIEIVPAFKARDVGIDRSMVGGYGQDDRVSAYTLLKGLCQVRDPRRHCLAIFT
                     DKEEIGSEG"
     gene            complement(922..1276)
                     /gene="ssrA"
                     /locus_tag="PKJ17_00010"
     tmRNA           complement(922..1276)
                     /gene="ssrA"
                     /locus_tag="PKJ17_00010"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1278..1724)
                     /gene="smpB"
                     /locus_tag="PKJ17_00015"
     CDS             complement(1278..1724)
                     /gene="smpB"
                     /locus_tag="PKJ17_00015"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOD74374.1"
                     /translation="MKVICTNRKAYHEYHIEEKFETGIVLTGTEVKSLREGRANLKES
                     YAKVKDGEVFVVNAHISPYSCGNIYNHEPKRTRKLLMHKREIMRLIGKVKERGYTLIP
                     LSLYFDRRNRAKMELALAKGKTLYDKREGIRRKDEKRLMEREERMR"
     gene            complement(1944..4070)
                     /locus_tag="PKJ17_00020"
     CDS             complement(1944..4070)
                     /locus_tag="PKJ17_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD74375.1"
                     /translation="MKLKKELSIIIGILVAFGALVLIESSLPNFARFFPVWENKLLVV
                     VLNINLLLILLLLFLITRIILKAYIEKKRGIWGSGLKKRLTSTLLFISIVPSFTLFIL
                     ATGFFYVSMDRWFSQQVEDTMESALELSRLHYEEVFQRHTRIGEQMAEEIGKKELLED
                     EKGLRKLLRQYERTHLLGYVAVYDPRSAAMVRVGGSAKSERTILSKLKLFTGKEVSQE
                     IIPDEQGEMLVVGMRLAPAGDSAPILFLGDTIRIQGKERMRQIAVTSKEFRESRVFKK
                     VLKYSFIIPLFLITILTIFVSLWVGIKMATEITVPIEKMKEGAAIIATGKFDINLEDR
                     GKDEIGTLVKAFNTMAKELKIAKDEIEDKSRSLEFVLNNVAAGIISTDKKGTILLANN
                     AARVILGVEREKLEGMSLREVFGNDFKALTKSFLREVRASKGESITKDLRLNLKKDIT
                     YLRASLTALKDERRRVAGFIIAFDDITHVVRAEKLATWQEVARKLTHEIKNPLTPIVL
                     SAERIRRKLMARFTGPDKGVLDETTSVIIRSVADIKDLVNELTKLTHTSQARTLEDLN
                     GVVDETLDLYRNLYHNISIGFEPGELPLVRVDRDGLKRAFINLITNAVKAIDGKAGTI
                     TVRTRYDRAKRAGIIEFTDSGKGVPDQDKEKVFDPYFTKDEGGTGLGLAIVHSIILEH
                     HGRIRVEDNVPEGAKFIIEIPITETA"
     gene            complement(4272..4988)
                     /locus_tag="PKJ17_00025"
     CDS             complement(4272..4988)
                     /locus_tag="PKJ17_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOD74376.1"
                     /translation="MKEFVLENRNDWEYYHVPGLTDAGILHGFFTRRSPASPLRGREA
                     EAFLDAFSLSDAVSLSQEHGTDIHVIANGDRPSKGDGILLYKRGIAGVINTADCLCVI
                     LVDPGFPMVSIVHAGWRGTLKKITSRAVGMMTERGAATQRMLALMGPSIRGCCYEVGR
                     EVTDAFLDAGFPGNILTSVNGSLHLDLEAANAHLVLEEGVTSIFDTGLCTCCSGDDFV
                     SYRRGARNARQMNFVAIRDR"
     gene            complement(5041..6000)
                     /locus_tag="PKJ17_00030"
     CDS             complement(5041..6000)
                     /locus_tag="PKJ17_00030"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOD74377.1"
                     /translation="MTDSFRIVCDVSDVRLDVFLSEKLSLTRTKVKEMIDGGHVSVDG
                     RTPKPAMRLKERMEIAGEVPRETALDVAPEEIALDILYEDDHFLAVNKPAGMVVHPSF
                     GHSSGTLVNAVLGYLERLGSRFKVQGSREQEPCSESDMAGVRPGIVHRLDKGTTGVIL
                     VARDERTQERLSVLFKDRKVRKTYRAVVEGTPGPDSWTVEGNIGRHPVERKKMAVLRS
                     GGRQAQTSFKVLERLEGFSYVEAYPTTGRTHQIRVHLNHAGYPIVGDETYGRRAKRSA
                     ARPLLHAWRIEFIHPATLKPVAIEAPVPADMIDFVEKHRGQLH"
     gene            complement(5990..7384)
                     /gene="selA"
                     /locus_tag="PKJ17_00035"
     CDS             complement(5990..7384)
                     /gene="selA"
                     /locus_tag="PKJ17_00035"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOD74378.1"
                     /translation="MPNNLLRQIPKVDSILESAGWASLVSVCPEPIAKDVLREHLDSL
                     RLSIREGRVVAVPSIDEIVTAVGIRAAVLIEPGLKRVINATGIIIHTNLGRSLLASRA
                     VNAIMRVATGYSNLEYDIEKGERGSRYDHCRSVLRTLTGAEDALVVNNNAGAVFLILN
                     TLAEGKEVIISRGELIEIGGSFRIPDVMKKSGAVLREVGTTNRTYIEDFERAIGDNTG
                     LIMKAHTSNYRIRGFAHEASSEELVALGKRYNIPTYYDTGSGLFYNFEDLGASGEPLI
                     PQELKKGFDVISFSGDKLLGAPQAGIVIGEKGCLGAMKQNPMTRALRPDKFTLAGLEA
                     TLLLCLDAGSAAGIPTIRMILEEKGAVGKRARRVASQLRKRCPDAGVSVIDVDSEVGG
                     GSFPDMVIPSVGVALESRTMTIAALEAKLRHLPLPVIARIEKERLILDLRTVLAEDEA
                     DLVAAVARAFENDR"
     gene            complement(7458..9509)
                     /locus_tag="PKJ17_00040"
     CDS             complement(7458..9509)
                     /locus_tag="PKJ17_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOD74379.1"
                     /translation="MEKFFYPESVAVFGVSEAQSNLARVIVENLLRFGFRGGIYPVGT
                     ARGVVAGIEIKENLDGVAAAPDLAVVLVPARYAPQTLEACGEKGIHNVVLESGGFSEL
                     SGDRETLEAEVLSIARRHGMTFVGPNCFGVVNLEAGVVLPFFIIDPEYMKEGSVSLIS
                     QSGGVFYDTCMLCSVENVGLRKLVSIGNKLMTNENDVLEYILKDGGTAVVGLYLENFS
                     DGRRFMALAASSDKPIVVLKANRSPASGEIAKFHTTALAGDDDVADAAMRQAGVIRVA
                     NFQEMVDCFKIFSLPVLKGRRLALISRSGGHGVLSADAARRYGFEFAPLSDGFFEGIA
                     RKKLNVIAATNPLDIGDVYDLGEYGSILEMALREDEVDGVVFIVTYSSESDGGKVRDF
                     LRFASRITPLYDKPVALCVVTNRAEWFAVKEAADLPVFTDVDQAMRALAWSYGHCLAK
                     SCGPRAFPEDFARLASGVPPDDGSGRRFLEPEECFSLLSRWGLPVADHVAARDRDEAL
                     AGAARLGYPVALKAGEGGLLHKSEAKGVLLGIEGPEDLLNALDSMEAKRYLIQRMAPP
                     GREVIVGGRFDNEFGPVVVCGLGGIYVEILADRSIRVAPVDDETAGRMIAELKGIAIL
                     EGARGRKPADIPALRDVIVRLSALLAENPRIVNLDINPVIVYDEGEGCAIVDAKVEMT
                     D"
     gene            9738..10178
                     /locus_tag="PKJ17_00045"
     CDS             9738..10178
                     /locus_tag="PKJ17_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOD74380.1"
                     /translation="MTRKEKALEAFASGLNCAQSVLLAFADEPGLDPEKGLKMASSFG
                     GGMGAMGLTCGAVTGAFMVIGLDSGYASSVDRPAKETMYGAVQGFAREFTEAHGSIAC
                     RELLGCDISTLAGYHEAIEKDLFHTVCPKYVETAVVILERTLGR"
     gene            10247..10645
                     /locus_tag="PKJ17_00050"
     CDS             10247..10645
                     /locus_tag="PKJ17_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74381.1"
                     /translation="MRKLAAILTVTVCLSLAVPTIGEARRYYYGPYRGCCGCSGDYWV
                     PAAVLAGTVLAGALVIGAMSQSSRQPTQPTVSQPAYRPIDTRQPYASPDPDFVTRYSK
                     KETSGQWTIVPAQKVGDTWVPAHRVFVPDP"
     gene            complement(10690..11862)
                     /locus_tag="PKJ17_00055"
     CDS             complement(10690..11862)
                     /locus_tag="PKJ17_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD74382.1"
                     /translation="MRLAIVVILCVVCSGVSSPSAVCASEACRDLVMQGRFSDAVREC
                     TGLIGSGKLSKEGLGAEHAWRGFAYMGAGDAGKAMGDFDRAIEMDPKNDMAYLGRGQI
                     HEMKKDYGAARVDLSRAIEINPGNSAALAHRGMISYVEKKYGDALGDLNRSIELDPKN
                     ARALCFRGNTHAAMKEQQKAVDDYTRALELNPGDFLSLLGRGLVQKKMKRYDEALADY
                     NGLIKVAPGFWQAYQGRADIYAATKRYGRAIEDLSRAIRIDPAQVVLYSQRGLTYVFY
                     RRPDKAKEDFSKAIELSPSSAALYLTRAALYREVGRLDESVGDLKKALELKPGNLATM
                     MLLARVESLRNNASEACRWLNAAYATGGLRNRNLVTREKDLDNIRYSECYMKFMER"
     gene            complement(12046..13506)
                     /locus_tag="PKJ17_00060"
     CDS             complement(12046..13506)
                     /locus_tag="PKJ17_00060"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOD74383.1"
                     /translation="MDASIIPACEFRWIMPEIMLTGWGLLLLIIGGLTRGPVASKTCG
                     VLSVIGSLCAMYFTVNLWGANQDVFNQLYTIDNYGTFFKGLFIAILILVSVVSLRYAD
                     REEIGSGEYYALLMFGVLGMMVMTSSHHFVTIFIGLEVMSLSIYVLCGLLRGNIASVE
                     AALKYFLLGAFATAFLLYGMAMIYGSTGLMDVAELKKFFLMKQPFSATMFIIGLGFLI
                     VGFGFKIASVPFHMWTPDVYQGAPTSVTAYMATGVKAAAFGALIRVFFTAFVPFQFGW
                     SEIIWILAVLTMCVGNITALVQRDIKRMLAYSSIAHAGYILVAFITGDRALASSMLYY
                     LMAYSFMNIGAFTVVIVLQKKGDNNSDLDSYAGLGARHPFIALCMSIFLLSLAGVPPL
                     AGFMGKFYVFSTAVNAQYYWLAIIGFLNSVVVAYYYLRVMMYMYFRETIGEPGTVDKA
                     PSYGLVMLICIWALLHMGIFPKTFLLIAQKAVSIFG"
     gene            complement(13519..15093)
                     /locus_tag="PKJ17_00065"
     CDS             complement(13519..15093)
                     /locus_tag="PKJ17_00065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOD74384.1"
                     /translation="MTVMSVPILSFLIYFPLLGAVILLFANRRNAQFIKVFTLVISLI
                     ELAVSIPLFFKFDDAAKTMQFVEKADWFPEWGISYFLGIDGISLLLVLLTTFLTVICV
                     LCSWKAIEDRVKEYYITFLFLETAMIGTLCALDLVLFYVFWELMLIPMYLLIGVWGGP
                     RRIYAAIKFFLFTMAGSVLMLVAILTLYFFYYNSTGVYTFNVLELYKASVPIAKQYWL
                     FGAFALSFAIKVPMFPVHTWLPDAHTEAPTAGSVILAGVLLKMGTYGFIRFAIPLFPA
                     AAIDAIPLMSILAVIGIIYGAIVSMMQPDLKRLIAFSSVSHLGYVMLGIFAFNMQGVQ
                     GGIYQMLNHGISTGGLFLIVGMIYERRHTRMISDFGGLSKVMPIFAVFFMIITLSSIA
                     LPGTNGFVGEFLILLGAFKNNVVYGTLATTGVILGAVYMLWMFQRVMFGVVTKEENRN
                     LKDLNGREILILVCMVFFIILMGVYPKMFFKKMDTSVAEYLAFVKQKSAQARVLEGNT
                     VPGKMAGEVANTNMTR"
     gene            complement(15104..>16101)
                     /locus_tag="PKJ17_00070"
     CDS             complement(15104..>16101)
                     /locus_tag="PKJ17_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOD74385.1"
                     /translation="GAYAAGIFHLMTHAFFKGLLFLAAGSVMHAMSGELDMRKMGGLR
                     KKIPITYWTTLIACLAIAGVPGLSGFFSKDEILWMAFSRYHSFWFWALGAVTAGLTAF
                     YMFRMFFNTFHGECRASEEVKHHIHESPKVMTIPLMILAVLSIVGGYVGVPHLLGGSN
                     HIEKWLEPVFGHHAPAAATHSGGFSIVSTAFASGAEGGGAEASAEMQLMIGAIIIGFL
                     GIFIAWLFYMKYPNLPKRFVEKFHGLFTLVHNKYFVDELYGFIFVKGLFKLGKVCKDF
                     FDETIIDGIINGIAAVLSGIGSLIRKIQSGFVQGYAFAIIFGAIIVIGYLISRIL"
BASE COUNT         3280 a         4988 c         4461 g         3372 t
ORIGIN      
        1 agccctcgga gcctatctcc tccttgtccg tgaagatcgc gaggcaatgc ctccgggggt
       61 ccctgacctg gcataaccct ttgagcaggg tgtatgccga gaccctgtca tcctgtccgt
      121 agccgccgac catactgcgg tcaatgccga catccctcgc cttgaaggcg ggtacaatct
      181 cgatctcggc gctgatgaga tcgtcctcgg tcatgccgta ggcatcgccc agtatcttga
      241 ggacgtattg tttcacgggc tccttttccg cgccggtcag cggcagcgaa ccgaagagga
      301 cgacgagctt ctccccgtcg atggcatcgc cgagcttctt ctcatcctgc gtcttcctgg
      361 acaggtgggg cagcaggtca tcgatgagga agacgggatc atcctccttt tcgccgatgc
      421 agatgtcgac ggtttcccca tctgacctca ccacaactcc atgaatggca agcgggaccg
      481 cgacccactg gtacttcttg atgccgccgt aatagtgggt cctcaggagc gccaggtcga
      541 tatcctcgta gagggggttc tgcttgaggt cgagtctcgg ggcatcgata tgggccacga
      601 cgatgttgag cccttcggag atgtcaccgc tcccccggac gaaggccatc acttccttgc
      661 ccctgttgac ggcaaacaca cgttcccccg aggggtcagt cccaaagccc tctgcttcga
      721 gggtccgaat gatggaccgc acggcctccc gttcggtctt cgcctcgtcg agaaaggcct
      781 tgtacccttc ggcgaagctg taaatctcct tgatgtcctc ttttgacttc gatctccagc
      841 cggattgtct ttgcatgggg tcctttcagg gtatcaaaat agcgcgggcg cgtcaacccg
      901 gggtaccgga gggttgatat atggtggagg cggtgggaat cgaacccacg tccagaaaag
      961 aaggcttcag acagactaca tgcttagtca gcgttttaga tctcggacag ccaggcaccc
     1021 gctgacgggt tcctcactgc cccagcctac aaggtttcgc ccggcgctat aggcgttacg
     1081 ccggactatc ccgctaagat gacaccatac acggacctaa agcaggagga aggtccggat
     1141 gatgtggctg cttactaggc agccagtgcg tactcgtgag agtctgcagt tgtgtttagt
     1201 tccgcttggg tacgggtaag cagtaacccg gcatgcctct ctgaccctca cttccctgtc
     1261 gaagccagtc gcccccgtca tcgcattctt tcctcacgtt ccatgagacg tttctcgtcc
     1321 ttgcgcctga ttccctccct tttgtcataa agtgtcttcc cctttgccag tgccagttcc
     1381 atcttggccc tgttcctcct gtcgaagtaa agggaaaggg ggatcagcgt atatcccctc
     1441 tccttgacct tgccgatgag ccgcatgatc tcccttttgt gcatgaggag cttgcgggtc
     1501 ctcttcggct cgtggttgta tatgttcccg caggaatagg ggctgatgtg ggcgttcacg
     1561 acgaaaacct cgccgtcctt gaccttcgcg tagctttcct tgaggttcgc cctgccttcc
     1621 ctgagtgact tcacttccgt gcccgtaagg actatccccg tctcgaactt ctcctcgata
     1681 tggtactcgt gataggcctt cctgttcgtg cagataacct tcataggcaa taatataaca
     1741 cagaaaggcg ggaaaagaca ggtctgccgg gctccggccc ggcagaccgc ttgaacagct
     1801 tgaacagctt gaaccgtagg aaagcgggta aggcatctac gcaagcagtg tccccggtcg
     1861 atggtattgt gccatgccgc cgggactttc cttcgtcttt taacgttcaa gcggttcaag
     1921 cggctgcaac tgtcttttcc ggttcaagca gtctctgtta tgggtatctc tatgatgaac
     1981 ttcgcgcctt ctggcacgtt gtcttcgacc cgtatcctgc cgtggtgctc gaggatgatg
     2041 gagtggacga tcgcgagacc gaggccggtg ccgccttcgt ccttcgtgaa gtaggggtcg
     2101 aagaccttct ccttgtcctg gtcggggaca cccttgccgc tgtccgtaaa ttcgatgatg
     2161 ccggcgcgct tcgccctgtc gtaccgcgtg cggaccgtga tggtccccgc tttgccgtcg
     2221 atggccttga cggcgtttgt gatgaggttg atgaaggccc gtttcagccc gtcgcggtcc
     2281 acacgcacga gagggagttc cccgggctcg aaacctatcg atatgttgtg atagaggttc
     2341 cggtagaggt ccagggtctc gtcaacgacg ccgttcaggt cctccagggt cctcgcctgg
     2401 gatgtgtggg tgagcttggt aagctcgttg accaagtcct tgatatcagc cacggaccgg
     2461 atgatgaccg aggtcgtctc gtcgagaacg cccttgtcgg gccctgtgaa ccgcgccatc
     2521 agcttcctgc ggatgcgttc ggccgacaga acgatgggcg tgagagggtt cttgatctcg
     2581 tgggtgagct tgcgcgccac ctcctgccat gtagccagct tctccgcgcg caccacgtgg
     2641 gtgatgtcgt cgaaggcgat gatgaatcct gccacccttc tgcgctcgtc cttaagggcc
     2701 gtgagcgaag ccctgaggta tgttatgtcc ttcttgaggt tgagccttaa atccttcgtg
     2761 atgctctcgc ccttcgaggc cctgacctcc ctcaggaacg acttcgtaag cgccttgaaa
     2821 tcgttgccga agacctctct cagggacatc ccctccagct tttccctctc gacgccgagt
     2881 atgacgcgcg cggcgttgtt cgcgagaagg atggttcctt tcttgtccgt cgagatgatg
     2941 ccggcggcca cattgttgag gacgaactcc agggagcggc tcttgtcctc tatctcgtcc
     3001 ttggctatct tgagctcctt cgccatggtg ttgaaggcct tcacgagcgt gccgatctcg
     3061 tcctttcccc tatcctcgag attgatgtcg aacttccccg tggctatgat ggccgcgcct
     3121 tccttcatct tctctatggg gacggtgatc tccgtggcca tcttgatgcc cacccagagg
     3181 gagacgaaga tggtgaggat ggtgatgagg aaaagaggaa tgatgaaact gtacttgagg
     3241 accttcttga acacgcggga ttccctgaac tccttgctcg tgacggcgat ctgcctcatg
     3301 cgctccttgc cctggatcct tatcgtgtca ccgagaaaga gtatgggagc gctgtccccc
     3361 gccggcgcga ggcgcatgcc caccacgagc atctcgccct gctcgtcggg gatgatctcc
     3421 tgggaaacct ccttccccgt gaagagcttc agtttcgaaa ggatcgtcct ttcggacttc
     3481 gcggagcccc cgaccctgac catcgccgcc gaccggggat cgtaaacggc cacgtagccg
     3541 agcaggtggg tccgctcgta ctgcctgagg agctttctga ggcctttctc atcctcgagc
     3601 agttcctttt tcccgatctc ctccgccatc tgctccccga tcctcgtgtg gcgctggaag
     3661 acctcctcgt agtgaagccg ggaaagctcg agggcgcttt ccatggtgtc ctcgacctgc
     3721 tggctgaacc accggtccat gctcacataa aagaaacccg tcgccagaat gaatagggtg
     3781 aaagaaggca cgatggaaat aaagaggagc gtcgaggtca gcctcttttt gaggcccgac
     3841 ccccagatcc cccgtttctt ctcgatgtat gccttgagga tgatgcgggt gatgaggaac
     3901 agaagcagaa ggatgagcag gaggttgatg ttcagaacga cgacgagaag cttgttctcc
     3961 cacaccggga agaaccgcgc aaagttggga agactcgact cgatcaggac aagcgccccg
     4021 aaggcaacga ggataccgat gatgatcgaa agctcttttt taagtttcat aagaacgggt
     4081 cttgggctat gggtgatagg taatcggaaa taccctctcc tctttacctc ttaccccatt
     4141 acctgcaacc catcacccgt ctcttccttt tctcttccca tcgcctatca cctatcgccc
     4201 gcctcttctt tgtcctttcc cgctgcccat aacctatcac ccataacccg tcttttccct
     4261 ttcttctccc atcacctgtc tcttatcgcc acaaagttca tctgccgggc gttgcgggcg
     4321 cccctgcgat aggaaacgaa gtcatcaccg ctgcagcagg tgcacaatcc cgtgtcgaag
     4381 atcgacgtga cgccctcctc gaggacgagg tgagcgttgg cagcctcgag gtcgaggtgc
     4441 agcgaaccgt tgacggaggt caggatgttc ccggggaaac ccgcgtcgag gaacgcgtcc
     4501 gtgacctccc tgccgacctc gtaacagcag ccgcggatcg aggggcccat gagggcaagc
     4561 atgcgctgtg tcgccgctcc ccgctccgtc atcatcccga ccgcccggga ggttatcttc
     4621 ttgagggtgc cgcgccagcc ggcgtggacg atggacacca tggggaagcc ggggtcgacg
     4681 agaatgacgc acaggcagtc cgcggtgttg atcacccctg caatgccccg tttgtacaga
     4741 aggatcccgt cgcccttcga aggcctgtcg ccattagcga tcacgtggat atcggttccg
     4801 tgctcctggc tcagcgagac ggcgtcgctg agcgaaaagg cgtcgaggaa cgcctccgcc
     4861 tcccgtcccc taagcgggga cgcaggcgac ctccgggtga agaacccgtg caggataccc
     4921 gcatccgtca accccgggac atggtagtat tcccaatcat tcctgttctc gagtacaaat
     4981 tccttcacaa ccccacccca tccttacaga acacgtttca tgttgtagtt tacgggtttt
     5041 ctaatgcagt tgtccacgat gtttttcgac gaaatcgatc atgtctgcgg gaacgggcgc
     5101 ctcgatggcc acgggtttca atgtcgcggg gtggatgaac tctatccgcc aggcgtggag
     5161 aaggggcctc gccgcggacc tcttcgccct cctgccgtat gtctcgtcgc cgacgatggg
     5221 atagccggcg tggttcaggt ggacccgtat ctggtgggtc ctgcccgtcg tgggataggc
     5281 ctcgacgtag gaaaaaccct ccagcctctc caggaccttg aagcttgtct gcgcctgcct
     5341 tccgccggag cgcagcacgg ccatcttctt tcgctccacc gggtggcgcc cgatattgcc
     5401 ctcgaccgtc caggaatcgg ggccgggcgt cccctccacg accgcccgat acgtcttccg
     5461 gaccttcctg tccttgaaga gcaccgacag tctctcctgc gtcctctcat cacgggcgac
     5521 gagaatgacc cccgtcgtcc ccttgtccag ccgatgcacg atccccggcc tcacccccgc
     5581 catgtcgctt tcgctgcagg gttcctgctc tcttgatcct tgaaccttga accttgaacc
     5641 cagtctctcg aggtatccca gcaccgcgtt caccagcgtg cccgagctgt ggccgaaaga
     5701 cgggtggacg accatacccg ccggtttgtt gacggccagg aagtgatcgt cctcgtaaag
     5761 gatgtcgagg gctatctcct ccggcgccac gtcgagggcc gtctcccgcg ggacctctcc
     5821 cgctatctcc atgcgctcct tcaggcgcat ggcgggcttg ggcgtcctgc cgtcgacgct
     5881 cacgtggccg ccgtcgatca tctcttttac cttggtccgt gtcagggaaa gcttttcgga
     5941 gagaaagacg tcgagccgca catcgcttac gtcacagacg atcctgaaac tatcggtcat
     6001 tttcgaaggc gcgggccacc gccgcgacga gatcggcctc atcttccgcg aggaccgttc
     6061 tcaggtcgag tatgagcctt tccttttcga tccgcgcgat gacggggaga gggagatgtc
     6121 tgagcttggc ctccagggcc gcgatggtca tcgtccggga ctcgagtgcc acaccgacgg
     6181 aagggatgac catgtcggga aagctcccgc cgcccacttc ggagtcgacg tcgatgacgg
     6241 agaccccggc atcggggcat cttttcctca actgtgaggc gacccgcctc gccctcttcc
     6301 cgacggcccc cttctcctcg aggatcatcc tgatagtggg aatcccggcc gccgaaccgg
     6361 catcgaggca gaggagcaac gtcgcctcaa ggccggcaag ggtgaacttg tcggggcgca
     6421 gcgccctcgt catggggttt tgcttcatgg ccccgagaca gcctttctcg ccgatcacga
     6481 tccccgcctg gggcgctccc agcagtttgt cgccactgaa ggatatgaca tcgaagcctt
     6541 ttttcagttc ctgaggtatc aggggctcgc ccgaggcacc gaggtcttcg aaattgtaga
     6601 agagcccgct gcccgtgtcg taataggtgg gaatattgta cctcttcccc agcgcgacga
     6661 gctcctccga ggacgcctca tgcgcaaacc cccggatgcg gtagttgctc gtgtgggcct
     6721 tcatgataag gcccgtgttg tcaccgatgg cccgctcgaa atcctcgatg tacgtcctat
     6781 tcgtggtccc cacttccctg agcacggcgc cggacttctt catcacatcg ggtatccgga
     6841 aggaaccccc gatctcgatg agttcgcccc gcgagatgat cacctctttg ccttccgcca
     6901 gggtattgag gatgaggaag accgcgccgg cgttgttgtt gacgacgagg gcgtcctccg
     6961 cgccggtaag cgtcctcaag acggaccggc aatggtcgta ccggctgccc cgctcgcctt
     7021 tctcgatatc atactcgagg ttggagtagc ccgtggcgac cctcatgatg gcattgacgg
     7081 cccggctcgc gagaagcgat cttcccaggt tcgtatggat gataatacct gtggcgttga
     7141 tgaccctttt cagccccggc tcgatgagga cagccgctct tatgcccacg gcggtcacga
     7201 tctcgtctat ggagggcacc gcgactaccc gcccttcccg tatgctaaga cgcagggaat
     7261 cgagatgctc gcgaaggaca tccttcgcta tgggctcggg acagacggaa acaagggacg
     7321 cccagccggc gctctcaagt atggaatcca ccttcggtat ctgtcgcagc aggttgtttg
     7381 gcatatctca ggtaggttgt atcatatgaa gagatctttt acaaaccgtt ttgccagccg
     7441 gtggactcga ggtcgtctca atcggtcatt tccactttgg catcgacaat cgcgcaacct
     7501 tcgccctcat cgtagacgat caccgggttg atgtcgaggt tgacgatgcg cggattctca
     7561 gcgagaagtg ccgacagccg gacgatgaca tccctgaggg ctggtatgtc tgccggcttt
     7621 ctgccccgtg ccccttcaag gatcgcaatg cctttcaatt ccgcgatcat cctgccggcg
     7681 gtttcatcgt ccacgggcgc gacccgaatg gacctgtcgg ccaggatctc gacgtatatt
     7741 ccgccaagtc cgcaaacgac aacgggtccg aactcgttgt cgaagcgccc cccgacgatg
     7801 acctctcgac ccgggggtgc catcctctgg atcagatatc gcttcgcctc catcgaatcg
     7861 agggcattca ggaggtcctc ggggccctcg ataccgagca gcacgccttt cgcctccgac
     7921 ttgtgcaaga ggccgccttc gcccgccttc agggccacag ggtaccccag gcgcgcggcg
     7981 cccgccagcg cttcatcacg gtctcgcgcg gcgacatggt ccgcgacggg cagaccccat
     8041 cgtgaaagca gggagaaaca ctcctcgggc tcgaggaagc gtctgccgga cccgtcatcg
     8101 ggcggaaccc ccgacgccag gcgcgcaaag tcttccggaa acgcacgggg tccgcagctc
     8161 ttcgccagac aatgcccgta cgaccacgca agggccctca tcgcctggtc cacatcggtg
     8221 aagacgggaa gatcggccgc ctctttcaca gcgaaccatt ccgccctgtt cgtcacgacg
     8281 caaagggcca ccggcttgtc atacagcggt gtgatccgcg aggcgaaacg gagaaaatcc
     8341 ctcaccttgc cgccatcact ctcggagctg taggtgacga tgaagacgac cccgtccacc
     8401 tcgtcctccc gaagcgccat ctcgaggatg gaaccgtatt cgccgaggtc gtagacgtct
     8461 ccgatgtcca gcgggttcgt cgccgcgatg acattgagct tcttccgcgc tatcccctcg
     8521 aagaagccat ccgagagagg ggcgaactca aaaccgtatc ttctggccgc gtcggcgctc
     8581 aacacgccgt ggccgccgga ccgcgagatg agggcaagcc tccttccttt gagcacgggc
     8641 agactgaata tcttgaaaca gtccaccatc tcctggaaat tggccaccct gatgacaccg
     8701 gcctgccgca tggcggcgtc ggccacatca tcgtcgccgg cgagcgcggt ggtgtggaat
     8761 ttcgctatct cgccgctcgc cgggcttctg ttggccttga ggaccacgat gggtttgtcc
     8821 gacgaggccg caagggccat gaaccgcctg ccgtccgaga agttctccag gtagagcccg
     8881 acgacagccg ttcccccgtc cttgaggatg tactcgagaa cgtcgttctc gttcgtcatg
     8941 agcttgttgc cgatgctcac gagtttcctc aggcccacgt tctccacgga acagagcatg
     9001 caggtatcgt agaaaacgcc gccgctctgg gatatgagcg atactgaccc ctctttcatg
     9061 tattccgggt ctatgatgaa gaagggaaga acgaccccgg cctcgagatt aacaacgccg
     9121 aagcaattgg gcccgacgaa ggtcatgccg tgccgccgcg ctatcgagag cacctcggcc
     9181 tccagagtct cacggtctcc ggagagctcg ctgaagccgc ccgattccag gaccacgttg
     9241 tgtattcctt tctccccgca tgcctccagg gtttgagggg cgtaccgggc gggcacgagc
     9301 accacggcaa ggtcgggcgc ggccgccacc ccgtcgaggt tctcctttat ctctatcccg
     9361 gcaaccacgc cccgggccgt tccgacgggg tagatacccc cccggaagcc aaagcgcaga
     9421 aggttctcca ctatgacgcg ggcaagattg gactgtgcct cggaaacacc gaacacggca
     9481 acgctctctg ggtagaagaa tttttccatt ctgggtcctt gcagaaaaaa cggtctcaac
     9541 gcgctctagt ataacccgcg agagccgcga atacaaggtc tgccggggct cgccgtgttg
     9601 acaccgaaaa ccgtcccctg ttaccatagt gaaaagcccg aacggttctt accccttaat
     9661 atttcacatc gactaacggg ctcgcggaca cgagccgata acgcagggca gacaacggca
     9721 cgaaagggag gtacctcatg acaagaaagg aaaaggcact cgaggcattt gccagcgggc
     9781 tcaattgcgc tcagtccgtc ctactggcct tcgcggatga accggggctc gaccccgaga
     9841 agggtttgaa gatggccagc tccttcgggg gcggcatggg agcgatgggt ctcacctgcg
     9901 gcgcggtgac gggcgctttc atggtaatcg gcctcgacag cgggtacgcg tccagcgtcg
     9961 acagacccgc caaggagacc atgtacgggg cagtgcaggg tttcgcccgc gagttcacag
    10021 aggcccacgg atccatcgcc tgccgggaac tcctcggatg cgatatctca accctcgcgg
    10081 gatatcacga ggcgatcgag aaggacctct tccacaccgt gtgcccgaag tacgtggaga
    10141 ctgccgtcgt gatcctcgag agaacgttgg gacgatgagc attgaaatgc attggtataa
    10201 aggtgcttac attcacggag aagggtgata tacttatacg agaattatga gaaaactggc
    10261 tgcgatactt acggtcactg tctgcctttc ccttgctgtt cccaccattg gagaggcacg
    10321 gcggtactat tatgggccct acaggggatg ctgcggctgt tccggcgatt actgggtacc
    10381 ggctgcggtc ctggccggca cggtactggc cggcgccctg gtcatcggtg cgatgagcca
    10441 gagctcacga cagccgaccc agccgaccgt ctcccagccg gcataccggc cgatcgatac
    10501 ccgccagcct tatgcctccc ccgacccgga cttcgttacc cgctacagca agaaagagac
    10561 ctccggccag tggaccatcg tccctgccca aaaggtaggc gacacatggg ttcccgccca
    10621 ccgcgtattc gttccggatc cctgacaaaa tggctgcacc cgcttaaagc cgctcgagca
    10681 ccctctttcc taccgttcca tgaacttcat atagcattcg ctgtatcgta tattgtcaag
    10741 atctttctct cttgtgacga gattcctgtt gcgcaagcct cctgttgcgt acgcggcgtt
    10801 gagccacctg catgcctccg atgcgttatt ccggagagac tctactctcg cgagaagcat
    10861 catcgtggcg aggtttcccg gcttcagctc cagcgccttc ttaaggtccc ccacggattc
    10921 atcgagtcga cccacctctc tgtacagagc cgctcttgtc aggtagaggg ccgcactcga
    10981 cggactcagt tcgatggcct ttgagaagtc ttccttcgcc ttgtcgggcc gcctgtagaa
    11041 gacatacgtc aggccccgct gcgaatagag gacaacctgg gccggatcga tccttatcgc
    11101 cctcgacagg tcttcgatcg cccgcccgta tctcttcgtc gccgcgtaga tgtccgcccg
    11161 cccctggtag gcctgccaga aacccggtgc gacctttatc aacccattgt agtccgccag
    11221 ggcctcatcg taacgcttca tcttcttctg cacaaggccg cggccgagaa ggctcagaaa
    11281 atctccgggg ttcagctcga gcgccctcgt ataatcatcg accgcctttt gctgctcctt
    11341 catcgccgcg tgcgtgttac cccgaaaaca caacgcccgg gcattcttcg gatcaagttc
    11401 gatggatctg ttgaggtccc ccagggcgtc cccgtatttc ttttcgacgt aggagatcat
    11461 ccccctgtgc gccagggccg ccgaattgcc cggattgatc tctattgccc tggacagatc
    11521 cacacgcgcg gccccgtaat cctttttcat ctcatggatc tggccgcggc cgagatacgc
    11581 catgtcgttc ttcgggtcca tctctatggc cctgtcgaaa tcgcccatcg ccttgcctgc
    11641 atcgcctgca cccatgtacg cgaacccccg ccacgcatgc tcggccccca ggccctcctt
    11701 cgacaacttc ccggacccta tcagccccgt gcattcccgg accgcatcgc tgaaccgccc
    11761 ttgcatcaca aggtcccggc aggcttcgga ggcgcacacg gccgaaggcg aagagacgcc
    11821 ggaacagaca acacagagaa tcactacgat ggcaagcctc atatcgtcct cctgtcgcgg
    11881 gcatccagca ctccttcccg aatactctgc gcccgcgccc gccgtctttc cgaatggccc
    11941 ccgcggagat gtccaccccg ttcgcccgat aacgaaacgg tcggccgcaa agaaaacctc
    12001 acggccgacc gcatgatatg cttcagttac ctacctgtat cggatctaac cgaagatact
    12061 cactgccttc tgggctatca acagaaaggt cttcgggaag atgcccatgt gcaggagggc
    12121 ccatatgcag atgagcatga ccagaccata acttggggcc ttgtcgaccg tccccggttc
    12181 accgatggtc tctctgaagt acatgtacat catgacccta aggtagtagt aagcgaccac
    12241 aacgctgttg agaaagccga tgatggccag ccagtaatat tgcgcattga cggccgtgct
    12301 gaagacgtag aattttccca tgaagccggc cagcggaggt actcccgcca gcgacaggag
    12361 aaagatgctc atgcacagcg cgatgaacgg atgacgggcg ccaagccccg cgtagctatc
    12421 gaggtcgctg ttgttgtcgc ccttcttctg caggacgatg accaccgtaa aggcgccgat
    12481 gttcatgaag gagtacgcca tgagataata gagcatgctc gacgcgagcg ccctgtcccc
    12541 ggttatgaag gcgaccagta tgtatcccgc gtgcgcgatg ctcgagtacg ccagcattcg
    12601 cttgatgtcg cgctgcacaa gggcggtgat gttccccacg cacatcgtca gcaccgccag
    12661 tatccagatg atctccgacc agccgaactg gaaggggacg aaggcagtga agaaaacacg
    12721 tataagcgcc ccgaaggccg ccgccttcac acccgtcgcc atgtaggcgg tgacggaggt
    12781 cggcgcgccc tggtacacgt cgggcgtcca catgtgaaag gggaccgagg ctatcttgaa
    12841 gccgaatccc acgatcagga agcccaggcc tattatgaac atcgtcgccg agaagggctg
    12901 cttcatcagg aagaatttct tgagctcggc cacgtccata agtcccgtgg aaccgtagat
    12961 catggccatc ccgtaaagga ggaacgcggt cgcgaaggca cccaggagaa aatatttcag
    13021 ggcagcctcg acggacgcaa tgttgcccct caggagtccg cagagcacgt agatcgaaag
    13081 ggacatgacc tcaaggccga tgaagatcgt gacaaaatga tgcgacgatg tcatgaccat
    13141 catgccgagc accccgaaca tcaggagcgc gtagtattcc cccgacccta tctcctcccg
    13201 gtccgcgtag cgcagggaaa ccacggaaac cagaataagg atggctatga aaagaccttt
    13261 gaagaatgta ccgtaattat cgatggtata gagctgattg aacacatcct ggttcgcacc
    13321 ccagaggttc accgtgaagt acatggcgca gagggacccg atcacggaga gcacgccgca
    13381 ggtcttcgag gcgacgggcc ccctcgtcag accgccgatg ataagcaaca gaagccccca
    13441 cccggtcagc atgatctcgg gcattatcca tctaaactca caggcaggta ttattgaagc
    13501 gtccattaac aggcacccct atcttgtcat attcgtattc gccacttctc cggccatctt
    13561 ccccgggacc gtgttccctt cgagaacccg cgcctgggcg ctcttctgct tcacaaaggc
    13621 aaggtattcc gccaccgacg tgtccatctt cttgaagaac atcttcgggt acacacccat
    13681 aagaattatg aagaagacca tgcagaccag tatgaggatc tcccggccgt taaggtcttt
    13741 caggttcctg ttctcttcct ttgtcaccac cccgaacatc accctctgga acatccagag
    13801 catgtacacc gcgcccagta tgacacccgt cgtcgcgagc gtgccgtaga ccacattatt
    13861 cttgaaggct cccagaagga tcaggaactc gcccacaaaa ccgttcgttc ccggcaacgc
    13921 gatggacgaa agggtgatta tcatgaaaaa gaccgcgaat atgggcatca ccttcgagag
    13981 gccgccgaag tcggatatca tcctcgtgtg cctccgctcg tagatcatgc cgacgataag
    14041 gaagaggcca ccggtggata tgccatggtt cagcatctgg tagatacccc cctggacccc
    14101 ctgcatgttg aaggcgaata tgccgagcat gacgtagcca aggtgactca cactcgaaaa
    14161 ggcgatcagc ctcttgaggt ccggctgcat catgcttacg atggcaccgt agatgatgcc
    14221 gatcaccgcc agtatcgaca tgagcgggat cgcgtcgatc gccgcggccg ggaaaagagg
    14281 gatcgcgaac cggatgaaac cgtacgtacc catcttcagc agaacgcctg cgaggatgac
    14341 actgcccgcc gtcggcgcct ccgtatgggc gtcgggcaac caggtgtgga ccgggaacat
    14401 ggggaccttg atggcgaagg agagggcgaa ggccccgaaa agccagtact gcttcgcgat
    14461 ggggacgctc gccttgtaga gttcgaggac gttgaaagtg tacacgcccg tactgttata
    14521 gtagaagaag tacagcgtca ggatcgccac gagcatcaga acgctgcccg ccatggtgaa
    14581 aaggaagaac tttatggcgg cgtagatcct gcgcgggccg ccccacacac ctatgaggag
    14641 atacatgggg ataagcatca gttcccagaa cacgtagaaa agcacgagat cgagggcgca
    14701 cagcgtccct atcatcgccg tttcgaggaa aaggaaggtt atgtagtact ccttcaccct
    14761 gtcctcgatg gccttccagg aacagagaac acatatcacc gtcaggaatg tggtcaaaag
    14821 gacgaggaga aggctgatgc cgtctatgcc cagaaaatag ctgatgcccc attcggggaa
    14881 ccagtccgct ttttcaacga actgcatcgt cttcgccgca tcatcgaact tgaagaaaag
    14941 cggaatcgat acggcaagct cgatcaggga aatgacaagg gtaaagacct tgatgaactg
    15001 agcgttcctg cgattggcaa aaagcaggat cactgccccg aggagcggaa agtatatgag
    15061 aaagctgagt attgggaccg acattaccgt catgaacaca ccttcacaag attctcgaaa
    15121 taagatagcc gataacgatg atcgcaccga atattatcgc aaacgcgtag ccctgaacga
    15181 atccgctctg tatcttccgg atgagactgc cgatacccga cagcaccgcg gctatcccgt
    15241 ttatgatgcc gtcaatgatc gtctcatcga aaaagtcctt gcagaccttg ccaagcttga
    15301 acaggccctt cacgaatatg aacccgtaca attcgtccac gaaatacttg ttgtgcacca
    15361 atgtgaacag accgtggaac ttctccacga acctcttcgg gagattggga tacttcatgt
    15421 agaacagcca ggcgataaag attcccagga acccgatgat gatggccccg atcatcagtt
    15481 gcatctccgc actggcctcc gctcccccac cctcggcacc agacgcgaat gctgtgctga
    15541 cgatactgaa accgcccgaa tgcgtcgctg ccgccggcgc gtggtgcccg aacacgggct
    15601 cgagccactt ttcgatatgg ttcgaacctc ccagcagatg gggcactccg acatagccgc
    15661 cgacgatgct gagcaccgcc aggatcatca gaggtatcgt catgaccttt ggagactcgt
    15721 gtatatgatg cttcacctct tccgacgccc tgcattcgcc gtggaaggtg ttgaagaaca
    15781 tcctgaacat gtagaaggct gtgagcccag cggtgaccgc gcccagcgcc cagaaccaga
    15841 aactgtggta cctgctgaag gccatccaga gtatctcgtc cttgctgaag aaacccgaca
    15901 ggcccgggac gccggctatc gccaggcagg cgatcaaggt ggtccagtac gtgatgggta
    15961 tcttcttccg caggccgccc atcttcctca tgtcgagctc cccgctcatc gcgtgcatca
    16021 cgctgccggc cgcgagaaag agcagcccct tgaagaaggc gtgggtcatg aggtgaaaga
    16081 tgcccgcggc ataggccccg g
//
LOCUS       DAOPOA010000002        11485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_6479_length_11485_cov_3.961155, whole genome shotgun sequence.
ACCESSION   DAOPOA010000002 DAOPOA010000000
VERSION     DAOPOA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 11485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11485
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6479_length_11485_cov_3.961155"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PKJ17_00075"
     CDS             complement(<1..908)
                     /locus_tag="PKJ17_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOD74386.1"
                     /translation="MQNQAIHPRAGENRHRAVDDIRSVIKSLGLVFGDIGTSPIYTLT
                     VIFLLLKPTVDNVMGVLSLVVWTLIVLVSVEYAWLAMSLGEKGEGGTIILRGHLIRLL
                     KRGRGAVFVTILAFIGISLLVGDGVITPAISILSAVEGILLIPGLHNTERTTLILIAG
                     AIAIALFAFQRKGTGKVAGAFGPLMLLWFLVLAVSGVASIIRFPSVWKAINPWHAVTF
                     LYENGAAGFFVLSEVILCATGAETLYADMGHLGRKPIIQAWCFVFLALVLNYLGQGAF
                     ILSNPAESRNVLFGMAFYQSSLLYIP"
     gene            complement(1509..1991)
                     /gene="tsaA"
                     /locus_tag="PKJ17_00080"
     CDS             complement(1509..1991)
                     /gene="tsaA"
                     /locus_tag="PKJ17_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOD74387.1"
                     /translation="MKDEGLQFIVRPIGVIHSPFRTKEETPIQGRFRPDAVGSIEIYE
                     EYREGLKDIEGFSHIFLIYPFDRAGRVELVRPVLLDDEPHGIFACRYPARPNCLGLTI
                     VELIGRNGSTLEVAGIDVLDGTPLVDIKPYVARFDCFPHAKEGWFKGKENRPKPKGRE
                     "
     gene            complement(2095..4281)
                     /locus_tag="PKJ17_00085"
     CDS             complement(2095..4281)
                     /locus_tag="PKJ17_00085"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOD74388.1"
                     /translation="MPEKIELPITGMNCAACAARIEKVLNKMETVQEARVNFPLKKAV
                     ILPKTGLELKDVVSLIRDIGYDVDMEADVTVRARKEETELRRDFSWAISLTVPIMVFS
                     MWMALPWNNVILLVLTIPVQFYFGLRFHASALINLKHFTADMNTLISVGTSAAFFYSA
                     FVTFFPHVVRAAGVSAMTYFDSSAMIISLILLGRYFESKAKTRTYTAIKLLYELSPKE
                     CVLLKDGVETRVPTDALEVGDTVLIRPGEKVPIDGVVLTGSTYVDESMITGESMPAHK
                     KPDDEVIGGTINGKGSVTVRTTRTGSDTVLARVIRLVEEAQFTKAPVQRLADRVAGVF
                     VPSVIIVGICAFLVWFFFGPEPRITNAILSFVSVLIIACPCALGLATPTAIMVATGVG
                     ARRGILIKSAEALELTNRARNVLFDKTGTLTEGVIVLADVIPLASSDEKEVLRIAFNL
                     EKQSEHPFSDALRKKAQESGIPSLDVTDFEAVIGRGIKGRIGGADYRMGNRTFYEEEG
                     HILDNRTRDLYEEKERSAASPVLLWSETGLMAILTFSDRVREESIEVVRKLREMNVEP
                     VMITGDSENGARTISGRLGIERYFHRVLPDGKAAIVEEFRKKGVTVMVGDGINDAPSL
                     AAADIGVAMGKGTDIAIESADVVLMKGHLSRLVSLIRLSRKTMRVIKQNLFWAFIYNV
                     LGIPIAFGALYPFFGIRLEPVFGAVAMVISSISVVSNSLRLKFFKD"
     gene            4436..5089
                     /locus_tag="PKJ17_00090"
     CDS             4436..5089
                     /locus_tag="PKJ17_00090"
                     /inference="COORDINATES: protein motif:HMM:NF008102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOD74389.1"
                     /translation="MYSVFELVKMVLDFVIHIDVHLSELIRSYGLWTYLILVLIIFSE
                     TGLVVAPLLPGDSLLFAAGAFAARGDFNIFWLFVLLSIAAVLGDTVNYWVGSYVGPKV
                     FHNENVRFLNRKYLDRTHEFYEKYGGKTIIIARFVPIIRTFAPFVAGIGSMTYGKFIS
                     YNVIGGILWIGAFAYAGFFFGNIPMVKHNFTLVIFAIIILSVMPGVIEIIKHKMKKA"
     gene            complement(5235..11084)
                     /locus_tag="PKJ17_00095"
     CDS             complement(5235..11084)
                     /locus_tag="PKJ17_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD74390.1"
                     /translation="MKRFLSSLREDPEKTAAQCVQRLWQATGESPAEAGKCTALARLL
                     VDGLADSVEAMDPGRFMDSIDACRPDHATMPGPSPFPLSGIIATLEEMVTEQEFDAEQ
                     ARFLWRLFCMARGKTTAAAVSSVSEDESEPEITLAELKREVAERRRAEKELRESERRF
                     RDIAEFIPQIIFEMDADCLLTFVNRQAYDIYGYTPRDVKKGVMGLDLVHPSDLPRIRQ
                     TLQAIAEGKPSTGSEFTAVRKDGSTFPVIAHVARIMKGGRLAGWRGIIVDITERKQAE
                     EKLKESERKFRDIAELSPQVIFEQGPDGVVTYANKRGLELFGYTEADLTSGAVMGMDL
                     FPEHEHARVRENLAKMIAGERETTGNEYTARRKDGSTFPAIIYTIAIFSQDRLTGLRG
                     VLVDTTDLKKADRELRERERRFRDLAESVPQVIFEMNETRRITFANRQAFQIFGYDEE
                     DLSRGIDAFSLFVPEQLPAMSENLRRIARGEASTGNEYTALRKDGTSFPVIIHVAPIL
                     DEGRLVGFRGIIVDITERKKAEEELRESRQFLQDIADRIPGVIYQFYAKPGGEMGLSY
                     ISRTAKELFGIETDTSRPFERFLEGLSPDCREALLDSISEAVAKEQDWQFEGDFIKPA
                     GEVMSFAGVSRPSRVGDTLRFNGVLLDITSRKRAEEQLRESERKFRDIAELMPQVIFE
                     IDPKGSVIFVNKQAFEFFGYKEDDLPEGFNCFDHIAPEDRSRAIANLQKAAEDGPSVG
                     NQYAAVKKDGSRFPIIIYVTRIIKDGRLTGYRGIIIDISERTRAEEALKQGEKRLRQI
                     IDLVPHFIFAKDIDGRFILANKAVADNYGTTVEGLIGRMDASFSPSDEEVRHFRNDDL
                     DVMITGRRKYIPGESITDAQGNVRYLETVKIPFTFSGTQTPAVLGVSVDITERMEAER
                     AIARSEQELRTIIAASPIGIGRIRDRIIEWVNEAMCNITGYAIDELTRTSTRILYEKG
                     SEFDRVGALLYRDFQGETKWVRKDGQVLDVLVQLSPMEGGAYIFTTTDITRLKRAEST
                     LKFTQFSVDNAVDSIYWIDNTGSIAYVNNSACLSTGYDRHELLSMKIYEIEPDRPARD
                     WPRYWRNIVEKGSEQFESVYRRKDGTTFPVEVSVSYLPYDEFEYVCAFSRDITERRKA
                     EEALKESEERWQFALEGAGDGLWDWNAQAGTVYFSGQWKEMIGYADKEIGDSIEEWDT
                     RLHPDDRDDVHARLEDHLEGRSSVYISQYRMRCKDGTYKWILDRGKVIRWTGDGRPLR
                     VIGTHTDITEHKRTEERLRLDESRTEALLRIHQMGNRPLEEITAFTLEESIRFTKSAI
                     GYLAFLNDDESVLTMHSWSKKAMEECRIQDKPISYPLETTGMWGDPVRRRRPIITNDY
                     AAPGVTRKGHPEGHVEVRRHLGVPIFDGERIVAVIGVGNKENEYGESDINQLQLMAQG
                     MIRLLKQKAAQEALSVSESRLRAVIDSARDAIFIKDRDRRYIVANGAMSELFGIPMQK
                     ILGATDRELFPDKTADHIETVDGRVLKGETIEEEMARPVAATVYVFHTIKVPLKNDRG
                     EITGLCGIARDITERKHLESQLLQSQKMEAVGTLAGGVAHDFNNLLSAIMGYASLLQM
                     KMDKENPLYGYASQILVSSEKAANLTQSLLAFSRKQVINLKPVVINDTVEKLHRLLER
                     LIPEDVEFRIHRNSERLVVMADPGQLDQVIMNLVTNARDAMPRGGKLTITVDRATVGN
                     EFITTHGYGRAGEYALIAISDTGIGMDSRVLEKIFEPFFTTKGVGRGTGLGLAIVYGI
                     IKQHNGYIDVTSRPGEGSVFCVYLPLVWLEPAREERSAAIIGGTETVLIAEDNADLCQ
                     LSVQVLQDHGYTVLAANDGAMAVETFRRHRGEISLVILDVVMPRMNGKEACEAIRLID
                     PSTRVIFTSGYTDDIIQEKGMLNEEYDFLGKPITPATLLRKIREVLDRK"
BASE COUNT         2123 a         3348 c         3389 g         2625 t
ORIGIN      
        1 aagggtatat agagcaggga ggactggtag aatgccatgc cgaagaggac gttccttgac
       61 tccgccggat tgctcaagat gaacgcgccc tgcccgaggt agttgagtac gagggcgaga
      121 aagacgaaac accacgcctg gatgatgggt tttctcccga ggtggcccat gtccgcatag
      181 agtgtctctg ccccagtcgc gcagaggatg acctcggaga gcacgaagaa gcccgcggca
      241 ccgttctcgt acaggaaggt gacggcatgc cagggattga tggccttcca caccgagggg
      301 aacctgatga tcgaggccac gccggagacg gccaggacca ggaaccacag gagcatgagg
      361 ggcccgaagg ctcccgcgac cttccccgtg cccttcctct ggaaggcgaa gagcgctatg
      421 gcgatcgctc cggcgatgag gatgagcgtg gtacgctccg tgttgtgcag tccggggatg
      481 aggagaatgc cctccacggc gctgagtatg cttatcgcgg gagtgatgac gccgtcgccg
      541 acaaggagag atatgccgat gaaggccaga atggtcacga agaccgcccc tcttcccctc
      601 ttcagaagtc ggatcaggtg gcccctgaga atgatcgtcc ctccctcgcc cttttcgccg
      661 aggctcatgg caagccacgc gtactcgacg gataccagga ctatcagggt ccataccacg
      721 agggaaagga cgcccatgac gttgtcgacc gtcggcttga ggagcaagaa aatgacggtc
      781 agggtgtaga tggggctggt tccgatgtcc ccgaagacaa gtcccaggga tttgatcacg
      841 gacctgatgt cgtcgacagc acggtgccgg ttctcgcccg cccgcggatg gatggcctgg
      901 ttctgcattg gagtgtctta tgttacccaa tatcgacggg aatttcaatg cgcagaaggt
      961 gcgggtacat caaaacgggg ttcttcgcct ggcatttgcg gcacgcacct gaccacagta
     1021 gaagcatgcg agctactcgt tcccgtcaag atagatgagt tccttcccgc agataaaacg
     1081 atcattcttg tgatccatgt aatatccctt ctcccccggt cattcagccc ctgtcgggac
     1141 agcggcgagc tctcatttgc ccgaaaggat ttccttaact ctcaacaggc tctcgacaag
     1201 cccctctttt tgtcatcccg accgcctttc ttcctccccc gtcagtccgg cgaaggccgg
     1261 aatccaggaa agactggcag tagatgacca ctgaatggtg aaagatccga caatacctcc
     1321 cgcattccac tgttcctgcc gtatcctttt gagggctttc tgcttcaaca ggactcttcc
     1381 tggattccgg ccttcgccgg actgacggac tggaactgtg aaggactgac ggactggaac
     1441 ggaagccgga ccggggaccg acattgccag gggcctgtca ggcgattcaa gcggctgcgg
     1501 tgacgtgttc attcccttcc cttcggtttc ggtctgttct ccttgccctt gaaccatccc
     1561 tccttcgcgt gcgggaaaca atcgaagcgg gcgacgtagg gcttgatgtc gacaagcggc
     1621 gtgccgtcga ggacatctat gcccgcgacc tcgagcgtgc tgccgtttct tccgataagc
     1681 tccacgatgg tgagtccgag gcagttaggc cgcgccggat agcggcaggc gaatattccg
     1741 tgtggttcat catcgagaag aacaggccgg accagttcga cccgacctgc cctgtcaaag
     1801 gggtagatga ggaatatgtg ggagaacccc tcgatatcct tgagcccttc acggtattcc
     1861 tcatagatct ctatggaacc caccgcgtcc ggccggaacc tcccctgtat cggtgtctcc
     1921 tccttcgttc tgaaaggaga atggataacc cctatgggcc tcacaatgaa ctgcaatcct
     1981 tcgtctttca tctctgtcct ttcccaaaat gattgtttca gtccctgttc cttccttgtc
     2041 tttggtctct aacgcttaaa cggctcaagc gattcaagcg gttcaagccg tctctcagtc
     2101 cttaaagaac ttcagcctca acgaattgct caccaccgat atggaactta tgaccatggc
     2161 cacggcgccg aagacgggct ccagccttat gccgaagaag gggtagagcg cgccgaaggc
     2221 gatggggatg ccgaggacat tgtagatgaa cgcccagaaa aggttctgct tgatgacgcg
     2281 catggttttc cgggagagcc tgataaggct gacgagtcgg gacaggtggc ctttcatgag
     2341 caccacgtcc gccgattcga tggcaatgtc cgttccttta cccatggcga cgccgatgtc
     2401 cgccgcggcg agcgatgggg catcgttgat gccgtccccg accattacgg tcactccctt
     2461 cttcctgaac tcctcgacga tggccgcctt cccgtcggga aggacgcgat ggaagtagcg
     2521 ctcgatgccg agccgcccgc ttatggtcct cgccccgttc tcactgtcgc cggtgatcat
     2581 gacgggctcc acattcatct ccctgagttt tcgcaccacc tcgatagact cttcccgaac
     2641 cctgtcgctg aaggtgagga tggccatgag ccccgtctcg ctccacagga ggaccggcga
     2701 ggcggcggac cgctccttct cctcgtaaag gtcccgggtc ctgttgtcga gaatgtgccc
     2761 ttcctcctcg tagaaggtcc tgttccccat ccggtaatcg gctcccccga tcctcccttt
     2821 gatacccctg ccgatgaccg cctcgaaatc agttacgtca agggagggta tgcccgattc
     2881 ctgggccttt ttcctcaggg catcggaaaa gggatgctcg gactgtttct ccaggttaaa
     2941 ggcgatccgg agaacctctt tctcgtcgga ggacgcaagc ggtatgacgt cggcaaggac
     3001 gatgacgcct tccgtaaggg tgcccgtctt atcgaagagg acgttcctcg ccctgttggt
     3061 gagttccagg gcctcggcgc tcttgatcag gatccccctt ctcgcgccga ccccggtggc
     3121 gaccatgatg gccgttgggg tggcaagccc cagcgcgcag gggcatgcga taatgaggac
     3181 gctcacaaag gataggatcg cgtttgttat cctcggttca gggccgaaga agaaccagac
     3241 caggaaagcg cagatgccca cgatgataac gctggggacg aagacacccg ccaccctgtc
     3301 ggcgaggcgc tgcacaggcg ccttcgtgaa ctgtgcctcc tccacgaggc gtatgaccct
     3361 ggcgaggacc gtgtcggagc cggtccttgt tgtcctgacg gtgaccgagc ccttgccgtt
     3421 gatggtcccg cctatcacct cgtcatcagg tttcttgtgc gcaggcatgc tctcgcctgt
     3481 tatcatcgac tcatcgacat aggtgctgcc ggtaagtacg acgccgtcga tggggacctt
     3541 ctctccgggc cgtataagta ccgtgtcgcc cacctcgagg gcatcagtgg ggacccgtgt
     3601 ttcgacgccg tccttaagga gcacgcactc cttgggcgag agctcgtaga ggagcttgat
     3661 cgccgtgtag gtccgtgtct tggcctttga ctcgaaatac ctgccgagga ggataagaga
     3721 gatgatcatg gcgctggagt cgaaataggt catggccgac acgccggcgg cgcgcaccac
     3781 gtgggggaag aaggtgacga aggcgctgta gaagaacgct gcagatgtac ccacggatat
     3841 gagggtgttc atgtcggcgg tgaagtgctt gagattgata agggccgacg cgtggaacct
     3901 caggccgaaa tagaactgga cggggatggt gagcacgagg aggatgacgt tgttccaggg
     3961 cagtgccatc cacatggaga agaccatgat ggggacggta agcgatatcg cccagctgaa
     4021 atccctcctg agctctgtct cttccttccg tgcccggacc gtgacatcgg cctccatgtc
     4081 cacgtcgtac ccgatgtcgc ggatgaggga gaccacatcc ttcagctcga ggccggtctt
     4141 cggaaggatg accgccttct tgaggggaaa gttgacgcgc gcctcctgga ccgtttccat
     4201 cttgttgagc accttctcta tgcgggcagc gcacgccgcg cagttcatgc cggtgatggg
     4261 gagctctatt ttttcgggca tcctgttctc cttctgctga atcgcagcgt gtaaacagta
     4321 taagacagcg ttcgggcaat cacaagaaga tggcgtctgc accaattcat tgactcaaga
     4381 gggggcaaaa gtgtagtata cagggaattt tcccggctaa accgactcag gagggatgta
     4441 tagcgtgttc gaactcgtca agatggtgct cgatttcgtc atccacatag acgtccacct
     4501 gagcgaactc atacggagct acgggctttg gacgtatctc atcctcgttc tcatcatatt
     4561 ctctgagacg ggccttgtgg tcgcgccgct gcttcccgga gactcccttc tctttgccgc
     4621 cggggccttc gccgcccgcg gtgatttcaa catattctgg ctttttgtcc ttctctcgat
     4681 agcggccgtg ctgggcgata ccgtcaatta ctgggtaggg agctacgtgg gtccaaaggt
     4741 ctttcataac gagaacgtgc gtttcctgaa ccggaagtac ctggacagga cccacgagtt
     4801 ctacgagaag tacggcggca agacgatcat aatagcccgt ttcgtgccca tcatccggac
     4861 cttcgcaccc ttcgtcgccg gcatcggcag catgacctat ggcaaattca taagctacaa
     4921 cgtcatcggc ggcatcctct ggataggggc cttcgcctac gccggcttct tcttcggcaa
     4981 tattcccatg gtgaagcaca acttcaccct cgtcatcttc gccatcatca tcctctccgt
     5041 catgcccggc gtcatcgaga tcatcaagca caagatgaaa aaggcgtaaa gatggttcca
     5101 gagttcaagg gcaaagacaa agaaagaggg cggacagcgc ggtacgggat ctccgttttc
     5161 ccggacgcag cgcaacggac catctcctca tcttttgctt tttactcttg aactcttgaa
     5221 ctctggaacc gtctttactt cctgtccagc acttccctta tctttctgag gagggttgcc
     5281 ggggttatgg gtttgccgag gaagtcgtat tcttcgttga gcatgccttt ttcctggatt
     5341 atgtcgtccg tgtacccgct cgtgaagatg accctggtgg acgggtcgat gaggcgtatg
     5401 gcctcgcagg cctccttgcc gttcatcctg ggcatgacga cgtcgaggat gacgagggag
     5461 atctcgcccc tgtgtcttcg gaaggtttcg acggccattg cgccgtcgtt ggcagcgagg
     5521 accgtgtatc cgtggtcctg aaggacctgg acggagagtt ggcagaggtc ggcgttgtcc
     5581 tcggcgatga ggacggtttc cgtgccgccg atgatggcgg ccgacctttc ctcacgcgcc
     5641 ggttccagcc acacaagggg gaggtacacg cagaagacgc ttccctcacc gggacggctc
     5701 gtgacgtcta tgtagccatt gtgctgcttg atgatgccgt agacgatggc gagtccgagg
     5761 ccggtgcccc gtccgacccc cttggtggtg aaaaagggtt cgaagatctt ttccaacacc
     5821 ctgctatcca tgccgatgcc tgtatccgag atggctatga gggcgtactc ccccgctctg
     5881 ccgtacccgt gcgttgtgat gaactcattg cccacggtgg ccctgtcgac ggtgatcgtc
     5941 agcttccctc cccggggcat ggcgtcgcgg gcgttcgtga cgaggttcat gatcacctgg
     6001 tcgagttgac cagggtccgc catgacgacg aggcgttctg aattcctgtg tatcctgaat
     6061 tccacgtctt ccggtatgag ccgctcgaga aggcggtgca gcttttccac ggtatcgttt
     6121 atgaccacgg gcttgaggtt gatgacctgt ttccttgaga aggcaaggag gctttgggtg
     6181 agattggccg ccttttcgga agagacgagt atctgggagg cgtagccgta aagaggattt
     6241 tccttgtcca tcttcatttg caggagactt gcgtatccca tgatggcgct caggaggttg
     6301 ttgaaatcat gcgcgacccc tcccgcaagt gtccccacgg cctccatctt ctgcgattgc
     6361 aggagttggg actcgaggtg cttgcgttcc gtgatatccc gggcaatgcc gcacaggccc
     6421 gttatttcgc cgcggtcgtt cttcagaggg accttgatgg tgtgaaagac atagactgtg
     6481 gcggccacgg gacgtgccat ttcttcctcg atcgtctcgc ccttaagcac gcggccatcg
     6541 accgtttcga tgtggtcggc cgttttgtcg gggaacaatt cccggtcggt agcgcccagt
     6601 atcttttgca tgggaatgcc gaagagttcc gacatggccc cattggcgac gatgtagcgg
     6661 cggtccctgt ctttgatgaa aatggcgtcc cgggcgctgt cgatgaccgc ccgcaggcgg
     6721 gactcggata cgctcagggc ctcctgggcc gccttctgtt tcaagagccg gatcattccc
     6781 tgcgccatga gctgtaactg gttgatatcc gattccccgt attcgttctc tttgttgccc
     6841 acaccgatga cggcgacgat cctctccccg tcgaagatgg ggacgcccag gtgtcgcctc
     6901 acctccacat ggccctcggg atggcctttg cgggtaacgc ccggggcggc gtaatcgttg
     6961 gtgatgatcg gcctgcgtct acgcaccggg tctccccaca tgccggttgt ctcaagggga
     7021 taggaaatgg gtttgtcctg gatgcggcat tcctccatgg ctttcttcga ccaggaatgc
     7081 atggtgagaa cggattcgtc gtcgttgagg aacgcgagat agcctatggc gctcttcgtg
     7141 aaccggatcg attcctcgag tgtgaaggcg gttatctcct ccaggggccg gttgcccatc
     7201 tggtggatcc tgaggagggc ctccgtccgc gattcgtcga ggcgcagccg ctcctcggtc
     7261 ctcttgtgtt ccgttatgtc cgtgtgggtc cctatgacgc gaagcggcct gccgtccccg
     7321 gtccaccgta tgaccttgcc cctgtcgagt atccatttgt aggtgccgtc cttgcaccgc
     7381 atccggtact ggctgatgta gacggatgac ctgccttcca ggtggtcttc gagccgggca
     7441 tggacatcat ccctgtcgtc agggtgcagg cgtgtgtccc actcctcgat ggaatcgccg
     7501 atctccttgt ccgcatagcc gatcatctcc ttccattgcc cggaaaaata gaccgtgccg
     7561 gcctgcgcgt tccagtccca cagaccgtca ccggcgcctt cgagggcgaa ctgccatcgc
     7621 tcttcactct ctttcagggc ctcctcggcc ttcctgcgct ccgtgatgtc ccgggaaaag
     7681 gcgcagacgt attcgaactc atcgtatgga aggtagctga cgcttacctc gacggggaac
     7741 gtggtcccgt ccttcctgcg gtacacggat tcaaactgct cggagccttt ttcgacgatg
     7801 ttccgccagt accggggcca atcccgagcc ggacggtccg gttcgatctc gtagatcttc
     7861 atggagagaa gctcgtggcg gtcgtaccct gtagagaggc acgcactgtt gttcacgtag
     7921 gcgatgctcc cggtgttgtc gatccagtag atgctgtcca cggcgttgtc gacggagaac
     7981 tgggtgaatt tcagcgtgct ttcggcccgt ttcagccggg tgatgtcggt tgtggtgaag
     8041 atgtaggccc caccttccat cggcgacaat tggacaagca cgtcgagcac ctgcccgtcc
     8101 ttcctcaccc attttgtctc tccctggaag tcccgataga gcaacgctcc gacccggtcg
     8161 aattccgatc ccttctcata gagtatgcga gtgctcgtcc gggtgagttc gtctatcgcg
     8221 tacccggtga tattgcacat cgcttcgttc acccactcta tgatcctgtc gcggatccgg
     8281 ccgatgccga tgggcgaggc ggcgatgatg gtcctgagtt cctgctcgga ccgggcaatg
     8341 gcgcgctcag cctccatgcg ttccgtgatg tccacggaca cgcccaggac ggcgggggtt
     8401 tgagttcccg agaatgtgaa agggatcttg acggtctcga gatagcgcac attcccctgc
     8461 gcgtcggtga tggactcgcc ggggatatat ttcctcctgc ctgtgatcat cacatcgagg
     8521 tcgtcgttcc tgaaatgcct cacctcttca tcggatggcg agaagctggc atccatcctg
     8581 ccgatcagac cttccaccgt ggtgccgtag ttgtcagcga cggccttgtt ggcaaggatg
     8641 aaccttccat cgatgtcctt ggcgaagatg aaatggggca cgaggtcgat gatctgccgg
     8701 aggcgctttt caccctgctt gagggcctcc tcggcgcggg tgcgctccga tatgtctatg
     8761 atgatccccc ggtagcccgt gagcctgccg tcctttatga tcctcgtgac gtagatgatg
     8821 atggggaatc ggctcccgtc cttctttacg gccgcgtact gattgccgac cgaagggccg
     8881 tcttcggctg ccttttgaag gttcgcaatg gccctcgagc ggtcttccgg agctatgtgg
     8941 tcgaagcaat tgaacccctc gggaaggtca tcctctttgt atccgaagaa ctcaaaggcc
     9001 tgtttattca cgaagatgac ggagcctttc gggtcgatct cgaagatcac ctgcggcatg
     9061 agctccgcta tgtcccggaa cttccgctcg ctctccctga gctgttcttc cgcgcgcttg
     9121 cgcgatgtta tatcgaggag caccccgttg aacctcaggg tgtcgccgac ccgtgaggga
     9181 cgagacacgc cggcgaagga catgacctct cccgcaggtt ttatgaagtc gccttcgaac
     9241 tgccagtcct gttccttcgc gacggcctca ctgatcgaat cgaggagcgc ctcccggcag
     9301 tcggggctga gcccttcgag gaaccgttcg aaggggcggg acgtgtcggt ttcgatgcca
     9361 aagagctcct ttgcggtccg gctgatgtag gaaaggccca tctctccgcc gggtttcgcg
     9421 tagaactggt agatgacgcc ggggatgcgg tcggcgatgt cctgtaagaa ctgcctgctt
     9481 tcccgaagct cctcctcggc cttcttccgt tcagtgatgt cgacgatgat ccccctgaaa
     9541 ccaacgagac ggccctcatc caggataggc gcgacgtgga ttataacggg gaatgaggtc
     9601 ccgtcctttc tcagggcggt gtactcattg cccgtggaag cctccccccg tgctatcctt
     9661 cggaggttct cactcatggc aggaagctgc tccggtacaa agagggaaaa ggcgtcgatg
     9721 ccccgcgaga ggtcttcttc gtcgtatcca aatatctgga aggcctgcct gttggcgaag
     9781 gtgatcctcc tcgtctcgtt catctcgaag atgacctggg ggacggattc cgcgaggtcc
     9841 ctgaatcttc gttcccgttc gcggagctcc ctgtcggcct ttttcagatc cgtggtgtcg
     9901 acaaggacac cgcgcaggcc cgtcaatctg tcctggctga agatggcgat ggtatagatg
     9961 atcgcgggga aggtgctgcc gtccttcctt ctcgccgtgt attcgttgcc cgtggtctcc
    10021 ctttccccgg caatcatctt cgcgaggttt tcgcgcactc tggcgtgttc gtgttcgggg
    10081 aaaaggtcca tccccatcac ggcgccggag gtgaggtccg cctcggtgta gccgaagagt
    10141 tcaagccccc gcttgttggc gtaggtgacg acgccgtccg gaccttgctc gaagatgacc
    10201 tgcggagaga gctcggcgat gtcccggaac ttccgctcgc tctccttaag cttttcctcg
    10261 gcctgtttcc tctcggttat gtcgacgatg atgccccgcc acccggcgag tctcccgcct
    10321 ttcatgatgc gcgcgacgtg ggctatgacg ggaaaggtgc tgccgtcttt cctgacggcg
    10381 gtgaactcgc tgcccgtgga gggttttccc tcggctatcg cctgaagggt ctgtctgatc
    10441 ctcgggaggt ctgaggggtg gacgagatcg aggcccatga cgcctttctt gacgtcgcgg
    10501 ggggtatatc cgtagatgtc gtaggcctgc ctgttgacaa aggtcaacag gcagtcggcg
    10561 tccatctcga agatgatctg gggaataaac tcggcgatat cgcggaagcg gcgttcgctt
    10621 tcccggagct ccttctcggc ccttcttctc tcggcgacct ctctttttag ttccgcaagg
    10681 gtgatctcgg gttcactttc gtcctcagag acgctcgaga cggccgcggc ggtggtcttc
    10741 ccccgggcca tgcaaaaaag cctccagagg aatcgggcct gttccgcatc gaactcctgc
    10801 tccgtgacca tctcctccag ggtggctatg atgccggaaa gagggaacgg ggatgggccc
    10861 ggcatggttg cgtgatcggg tcggcaggca tcgatggaat ccatgaacct tcccggatcc
    10921 atcgcctcca cgctgtcggc aagcccgtcg acaagcaggc gggccaaggc ggtgcacttt
    10981 cccgcttcag cgggactttc tcctgtcgcc tgccacaggc gctggacgca ctgtgccgcg
    11041 gtcttctcgg gatcttcacg cagagatgac aagaatcttt tcattgaact ccaacggtac
    11101 tcctgcagtc aagggccggt ttgcgcggct tccttcggga cctgcgtatg ggactgtaag
    11161 tgttatcggc caggtggcgg aatgtttaac aacaacaagg agatggaagg ggaaaaagaa
    11221 catggaagta gtggggtggg atacgcggga cacgctggtc ggcgcgcggg gatgaaaaga
    11281 acaggcccgc gggtccgata agacccgcgg ggcctgccga aaggacatta ttttttcttt
    11341 gccgcttttt tcggttttgc ctgtttctcc ttcagggctg cccgggcggc tgccagtttt
    11401 gctatcgtga cgcggaaggg cgaacagctc acgtagtcaa agcccgtcct gtggcagaac
    11461 tcgacggaat tcggttcgcc gccat
//
LOCUS       DAOPOA010000003        11384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_6542_length_11384_cov_3.781005, whole genome shotgun sequence.
ACCESSION   DAOPOA010000003 DAOPOA010000000
VERSION     DAOPOA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11384
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6542_length_11384_cov_3.781005"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKJ17_00100"
     CDS             <1..299
                     /locus_tag="PKJ17_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOD74391.1"
                     /translation="AARPLPPGRLQPADLIFLTPAEGRGIDHVILYLGGERFVEAVET
                     GRPVEVGTFRTRFGHSREEMVRDGLSDWSGRIHFASLLEEGGNRKNGSEAYGDR"
     gene            286..1368
                     /locus_tag="PKJ17_00105"
     CDS             286..1368
                     /locus_tag="PKJ17_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOD74392.1"
                     /translation="MATDDIKTVRMAEVVRPLKTTFATSLGRKDAMRSVIVEVRLMDG
                     SSGFGECATSFVLPHETVDRIRAVIRAERPHLTGKRAADFSAIAAELRERHPAFPMTV
                     SGLETAIWRAWLRNTGTGETDWFGGALRRVETDITIPYTTDVMVLKGWIEQAARKGFA
                     VYKVKVSGRKADDRRLIASVASILEALDIRYTLRLDGNQGFTKTACLALADHVTKKKY
                     PVELFEQPLKKDDRRGLREITKRSPLPVILDETVFSAEDMEMALNDGLGHGVNIKTAK
                     SGITESLSIMKLGKQGGLRLMAGCMTETMTGLSAGIAMAMGTAAFDYIDLDSVHFLNH
                     RKQYGEIAVEGAGYRRQEMEDGGKVG"
     gene            1481..2806
                     /locus_tag="PKJ17_00110"
     CDS             1481..2806
                     /locus_tag="PKJ17_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOD74393.1"
                     /translation="MTSLNRVSPVERLGRSLRVAGIVLRESLRSFLGNNDFEMSAALA
                     TYSFFALVPLLFLVAAFAADTGGTATWLIAGIEGLIDHLFPRIRDWMSVEFSAAIGHP
                     LTLGGAAAVVVFIAAMSLVDSLRTAFLKIFRKNPPASLFTVQFGNVKRAAAMLLLFAV
                     LIGAEIAHLAIGNILRDRLLFTSVGTILVSICVAALCMAFFYRVFLPVRLTAFKLTAV
                     CVISAVLIILMREVFAFVLRTNPAYGETFGSLKVLFVMIVWVYYCFLAILFGAELAVN
                     TGKRDALLLKRLFAGNVQRALSKGLLARFIRSSAKNDVIFEEGDPGGAMYYIVSGSVD
                     IMRGGKHILTMHSGDYFGEISMLLGTPRSAAARAADEPTELVAISQGNFDLILRDNAP
                     IVLSLLKELAGRLKDTDESLLGRQRQKTTSRPFAAFAAHSSTRRLKRDR"
     gene            complement(3076..4290)
                     /gene="xseA"
                     /locus_tag="PKJ17_00115"
     CDS             complement(3076..4290)
                     /gene="xseA"
                     /locus_tag="PKJ17_00115"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOD74394.1"
                     /translation="MGVYPREDEGLLTVSELTAELRRFVSGRFRDVRVEGEVSNAKLY
                     PSGHFYFTLKDDLAMIRGVVFNYAARVPSGRVIKDGEQVLCRGRIDVYEKRGEYQLIV
                     SDILASGERGLAYLRFLELKEQLLREGLFDETLKKPLPRFPRCIGIVTSPVGAAIRDI
                     LRVIHERCGTVTVQVWPAAVQGDDAAREIMDGIGHFNRSGQVDVIIIGRGGGSYEDLA
                     CFNNEGLARAVFASRIPVVSAVGHEVDFTIADFVADVRAPTPTAAAALVVPDRTELIR
                     YVLGLKDSLCQAMEKALERARYMLVSNVMDFKDRKDFFTSHRLYLDDLTVGLTHNIQF
                     YMKNARTKFEALSQRIHDLNPTAILARGYSITSRADTGAVVTDSSAVSPGEALSITLH
                     KGGLDVSVVDRK"
     gene            complement(4291..5577)
                     /gene="thiC"
                     /locus_tag="PKJ17_00120"
     CDS             complement(4291..5577)
                     /gene="thiC"
                     /locus_tag="PKJ17_00120"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOD74395.1"
                     /translation="MKNQLIAAREGKITRVMERVARDEGLDKEHLRQHIAEGKATILA
                     NNRHRDFTPKGVGKGLSVKVNANIGTSPERVNIDEEIEKLTVAREAGADAVMDLSTGG
                     NLDEIRKIVIDAAKIPVGTVPIYQAAIETVKKRKAIVRMEADDIFEVIEKHGADGVDF
                     VTVHCGVTKSALERLRKQGRVTDIVSRGGAFLAQWIIVNGKENPLYENFDRLLTIAKK
                     YDMTISLGDGLRPGCIADATDRGQIEELIILGELCAEALDQGVQVMIEGPGHVPINQI
                     ETNMVLQKRLCNEAPFYVLGPIVTDIAPGYDHITSAMGGALAAYYGADFLCYVTPTEH
                     LGLPMPQDVRDGVVVTKIAAHAADLARGSKKAFALDKAMSTYRKALDWKGQIECAIDP
                     EKIRAFRKERNLKNDVCSMCGEFCSMKLMKDYLKKK"
     gene            complement(5585..6451)
                     /locus_tag="PKJ17_00125"
     CDS             complement(5585..6451)
                     /locus_tag="PKJ17_00125"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HOD74396.1"
                     /translation="MKNLVSIAGFDASSGAGVVRDVDTFFSFGFHGIAVPTCTVVQGP
                     GGVERVEPSSEDLFRDVLRAATAGIPVKGVKIGVLCDESHVRATASFLAKRRGAPVVL
                     DPVFAAKNGVPLITDEGRAACIDVLFEKVTVITPNAEEASAITGIKVGTVKAARKAAE
                     KIRSLGPGAVVIKGGHLEGDPVDVLFDGREYLLYRKARFPRSIHGTGCSFSSALACFL
                     AAGYPLSHAFMATQGYLERLFESSYRIDRKGHFYVSSATERNFDTAGQRMKIQGAHGK
                     PPEDPGPLRIWR"
     gene            6843..7310
                     /locus_tag="PKJ17_00130"
     CDS             6843..7310
                     /locus_tag="PKJ17_00130"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOD74397.1"
                     /translation="MKKILGVIVAVLFIAAASTVFAVGPGGYGPGPKGFGYGRGIDLS
                     KEQMDKMWQIRERFNTDTSSLRYELFQKRGELRTLYSDPKASDAAILAKEKEVDTLRQ
                     KMHDRMVRFKLEQRKVYTPEQLQKLAESGYGPGFGGGRGGRGGGRGLGPCGRF"
     gene            7378..7767
                     /locus_tag="PKJ17_00135"
     CDS             7378..7767
                     /locus_tag="PKJ17_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HOD74398.1"
                     /translation="MKNIVAVVLLATVTGLFLVSCASEGYNTQKGAAIGAGLGAIAGQ
                     VIGHDTASTLIGAAVGTLVGAVGGNAVDQHVQNQQRTSTQQSAAYPSSSQVTPQDQPP
                     GQWVEVPGQWIGGKWVPAHKVWVPVNP"
     gene            7764..8183
                     /locus_tag="PKJ17_00140"
     CDS             7764..8183
                     /locus_tag="PKJ17_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOD74399.1"
                     /translation="MTFVVFFLLLCVFITPSLRAQNSYSEFERGLQLTEPQRTQVEDI
                     RNRYINEWQSLRRESSRKQRELRELNRNPETNSARIGRIQGELQELDTARHNSYQQYR
                     SEVSRTLNRRQREQYNSFCDQERRKNVQRFRPGRHGR"
     gene            8173..9753
                     /locus_tag="PKJ17_00145"
     CDS             8173..9753
                     /locus_tag="PKJ17_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD74400.1"
                     /translation="MGDKRAVALPLFFALFLVLITLTGFFQIRIIRNNIERQLMGEAE
                     IIFTHVQREIDINLEYLSFLDRSPVIITPYFFNIMAFDESIIDDLDAVMGRFLKNPAE
                     RGLEELSLANVIEYDLTGKIVAEKGRSDIPAGLLQKIVSGEEATVIRMPVAGDRSLVF
                     GRRLGERILFVRINAPELETLRRKVILRDIVDREEKRFNIDSIRIYDEKGGLFVGTSD
                     AGKNTFLLERDLSSKVLPGFRMQIFISRNLARDTIQRSAIGFILILAVLFVSGAGSIY
                     AFFVMERKYAKRVKDMEREMEIKERLVSLGKLASGMAHEIRNPLNAISLSVQRLKREF
                     VPWDEKKEEYLTFLDIIRKELTRVNGIVEEFLQSTRAQAPFSREDLRDLLEEVIIIVG
                     EKASSKGIFIENATGTGIHVDCQRDRLKQAFYNIIINAIESMKDGGKIAITVKQRDSR
                     VEISVRDSGPGISEEALARIFEYYYTTKDKGMGLGLPISYVIVKDHGGDIKVTSAAGQ
                     GTTFVIVLPRTQDATTAA"
     gene            9776..11140
                     /locus_tag="PKJ17_00150"
     CDS             9776..11140
                     /locus_tag="PKJ17_00150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD74401.1"
                     /translation="MDRVGILIVEDEQTQRSLLGGLLRKEGYTIGEASDGESALALFQ
                     KDIFEIVLLDYRLPDTDGLSLLKRIKEINPATEVVMVTAFGSIENAVGALKAGASEYL
                     TKPIDLDDLLFKLRKVEEKTYLIRENMVLRETLRDRFKSEEFVYQSERMHEVSSLVVR
                     IAKTDSTCIISGESGVGKEVVLNMIHALSERKDFPLVKVNCAAIPETLLESELFGYEK
                     GAFTGAYQRKAGKFELANKGTIFLDEIGDIPLVLQSKLLRVIQEKEVERLGGAHPVKI
                     DVRIVAATNRNLEEEVKKGTFREDLYYRLNVVNIVIPPLRERREDIPLLIDFFLKKYN
                     LKHKKNVKGLTREARDTMVKYDYPGNVRELENIVERAIVLTRGDHITSQDLPNLAEQA
                     MVTGDGSIRGTVESIERSMIIEALINADWVQTKAAMALGLSERMLRYKIKKYGISRSS
                     RSAG"
BASE COUNT         2470 a         3356 c         3268 g         2290 t
ORIGIN      
        1 cggcggcaag accccttccc ccgggccggc tccagcctgc cgatctcata ttcctcaccc
       61 ccgctgaagg ccgggggatc gaccatgtga tcctctacct cggcggagaa cggttcgtcg
      121 aggcagttga gacgggcaga cctgtcgagg tggggacttt ccgaacacgt ttcggacaca
      181 gccgtgaaga gatggtgagg gatggcctct ccgactggtc cgggcgcatc cacttcgctt
      241 ccctcctgga agaaggagga aacagaaaga acggatcgga ggcctatggc gaccgatgac
      301 ataaagaccg tccgcatggc ggaagtggtc cgtcccctga agaccacctt tgcaacctcc
      361 ctggggcgta aggacgcgat gcggagtgtc atcgtcgagg tccgtctcat ggatgggtcc
      421 tcaggattcg gggaatgcgc cacaagcttc gtcctccctc acgagacggt ggacaggata
      481 cgggcggtca tccgcgcgga gaggccccat ctgacgggga aaagggcggc cgatttctcc
      541 gccatcgcgg cagagttgcg cgagagacac cccgcatttc ccatgaccgt ttccgggttg
      601 gagacagcca tctggagggc gtggctgcga aacaccggaa caggcgagac cgattggttc
      661 ggcggtgccc tgcgacgcgt ggaaacggat atcacgatac cttacacgac tgacgtaatg
      721 gttctcaagg gatggataga acaggccgcc cggaagggtt ttgccgtgta caaggtcaag
      781 gtgagcggca ggaaggccga tgacaggcga ctcattgcct ccgtggcatc gatcctcgaa
      841 gctctggata tccgttatac cctgcggctc gacggaaacc agggtttcac aaagaccgcc
      901 tgcctcgctc tcgccgacca tgtgacgaag aagaaatacc ccgtcgagct tttcgaacag
      961 cctctgaaaa aggacgatcg ccgcggcctc agggagataa cgaagcgctc tcccctcccc
     1021 gtcattctcg acgagaccgt attttcagcc gaggacatgg aaatggccct caacgacggt
     1081 ctcggccacg gcgtcaacat caagaccgcg aagagcggga tcacggagtc cctgtccatc
     1141 atgaagttgg ggaagcaggg cggcttgagg ctcatggccg gctgcatgac ggaaacgatg
     1201 acggggcttt ccgcgggcat cgccatggcg atgggaacgg ccgccttcga ttatatcgat
     1261 cttgactcgg tacacttcct caaccaccgg aagcagtacg gggagatagc ggtggaaggg
     1321 gcggggtaca gaaggcagga gatggaggac gggggaaaag ttgggtgagg ggttgatagg
     1381 tcctattctc ctataagtcc tatcatctgt cccgctcccc accccctgcc gcctcttccc
     1441 tgttccctga tccccgatca tctgctataa ttgaccatcc atgacatccc ttaacagagt
     1501 ctctcctgtc gaaaggctcg gccgttcgct gcgggtagcc gggatcgtcc tccgcgagtc
     1561 cctgaggtca ttcctcggca acaatgactt cgaaatgtcc gcggcccttg cgacatacag
     1621 ttttttcgcc cttgtccctc ttctcttcct cgtggccgct ttcgccgcag acaccggagg
     1681 caccgccaca tggctcatcg ccggcatcga gggtctcatc gatcacctct tcccccgaat
     1741 ccgggattgg atgagcgtcg aattctcggc ggcgatcgga caccccctga ccctcggcgg
     1801 ggccgccgcg gtcgtcgtct tcattgccgc catgtccctc gtggattccc tcaggacggc
     1861 ctttctaaaa atcttcagga agaacccccc ggcgtcactg ttcacggtcc agttcggcaa
     1921 cgtgaaaagg gcagcggcca tgctcctcct ctttgccgtt ctcattggag cggagatcgc
     1981 ccatctcgcc attggaaaca tcctgcggga tcgcctcctc ttcacgagcg tcgggaccat
     2041 ccttgtttca atttgtgtcg ccgccctctg tatggccttc ttttaccggg tcttcctgcc
     2101 cgtaaggctc acggcattca agctcacagc tgtttgtgtc atctcggccg tcctcatcat
     2161 cctcatgagg gaggtcttcg cctttgtcct caggacgaac ccggcttacg gggagacctt
     2221 cggctccctg aaggtccttt ttgtaatgat cgtgtgggtc tactattgtt tccttgccat
     2281 ccttttcggt gccgagctcg ccgtcaacac ggggaagcga gacgcccttc tcctgaaacg
     2341 cctcttcgcc ggcaatgttc agagagccct ctccaaggga ctccttgcca ggttcatcag
     2401 gagcagcgcc aagaatgacg tcatattcga ggagggggac cctggcggtg ccatgtacta
     2461 tatcgtctcc ggctccgtgg acatcatgcg cggcggcaaa cacatcctta ccatgcactc
     2521 cggagactac ttcggtgaga tatccatgct cctcggcacc ccgcggtcgg cagccgcccg
     2581 cgccgctgac gagcccacgg aactcgttgc catctcccaa gggaacttcg acctcatcct
     2641 tcgcgacaac gctcccatag tcctctccct cctcaaggaa ttggccggca gactcaagga
     2701 caccgacgaa tccctcctcg gccggcaacg acaaaaaacc acctcacgcc cgttcgccgc
     2761 tttcgctgct cactcgagca cgcggaggtt aaagagagat cgctgaaaaa agcactcccg
     2821 ggaattctcc caattcccgg gaaccttcga ccctgctctg acgagcagcg gagaagaaga
     2881 cccgatgaca agcatcgtta acaggcgtta cggaactgtc tttacctcgc gccggaacgg
     2941 cgggatgcga atgcggcctg atcccggcgg gcgatgggat caggccgcgc cttctctctg
     3001 tgccctctcc ttgcgcttct ccgtgtcctc gagtgaggag cgaaagcgac gaacgggcgt
     3061 gagacaaggt cttgattact tcctgtcgac cacggacacg tcgaggccgc ccttgtggag
     3121 cgtgatcgag agggcctcgc ccggcgagac ggcggaggaa tccgtgacga cggcgccggt
     3181 gtccgccctc gaggttatgg agtaacccct cgccaggatc gctgtgggat tgaggtcgtg
     3241 tatcctctgg ctgagggcct cgaacttcgt gcgcgcgttc ttcatgtaga actggatgtt
     3301 gtgggtgagg ccgacggtga gatcatcgag gtagaggcgg tgggaggtga aaaaatcctt
     3361 ccggtccttg aaatccatga cgttgctgac gagcatgtac cgggcccttt cgagtgcttt
     3421 ctccatcgcc tggcacaggg aatccttaag acccaggaca tagcggataa gctccgtcct
     3481 gtcagggaca acgagggcgg cggcggccgt cggagtggga gcacgcacgt cggccacgaa
     3541 gtccgctatc gtgaaatcga cctcatggcc cacggcagaa acgaccggta tccgggaggc
     3601 aaagacggct cttgccaggc cttcgttgtt gaagcaggcc aggtcttcat acgaaccccc
     3661 tccccttccg atgatgatga catcgacctg cccgctccgg ttgaaatgcc cgatcccatc
     3721 catgatctca cgggccgcat cgtcaccctg cacggccgcg ggccagacct ggaccgtgac
     3781 ggtgccgcac ctttcgtgta tgacccgcag gatgtcccgt atcgcagctc ccaccggaga
     3841 ggttactatg ccgatgcacc ggggaaaacg gggaaggggt ttcttgaggg tctcgtcgaa
     3901 gaggccttcc ctgagaagct gttctttgag ctcgagaaaa cgcaggtagg cgaggccccg
     3961 ctccccgctc gccaggatgt cgctcactat cagctggtat tcgccgcgtt tctcgtagac
     4021 gtcgattcgc cccctgcaca ggacctgttc gccgtccttt atgaccctgc ccgaggggac
     4081 gcgcgcggca tagttgaaga caacaccgcg gatcatggca aggtcgtcct tcagagtaaa
     4141 gtagaaatgt cccgagggat agagcttcgc gttggagacc tcgccctcga cacgaacgtc
     4201 acggaaccgg ccgctgacga acctcctgag ctccgccgtc agctccgata ccgtcagaag
     4261 cccttcatcc tcgcgcgggt agacgcccat ctatttcttc ttcaggtagt ccttcatgag
     4321 cttcatggaa cagaactcgc cgcacatgct gcagacatca ttcttgaggt tccgttcctt
     4381 ccggaatgcc cgtatctttt cggggtctat ggcgcactcg atctgccctt tccagtcaag
     4441 ggccttccga taggtcgaca tggccttgtc aagagcaaag gccttcttgc tcccgcgcgc
     4501 gagatcggcg gcatgggcag ctatcttagt aaccacgaca ccgtcacgga catcctgcgg
     4561 catgggcagg cccagatgct cggtgggggt gacgtaacag aggaaatcgg ctccgtagta
     4621 ggccgccagc gctcccccca tggcggacgt gatgtggtcg taacccggcg cgatatcggt
     4681 gacgatggga ccaagaacgt agaagggcgc ctcgttgcag agcctcttct gaagaaccat
     4741 atttgtctcg atctggttga tcggaacgtg gccgggccct tcgatcatga cctggacacc
     4801 ctggtcgagc gcttccgcgc agagttcgcc caggattatg agctcctcga tctgaccgcg
     4861 atcggtcgcg tccgcgatgc agcccggacg aagaccatcc ccgagactga tcgtcatgtc
     4921 gtatttcttc gcgatggtga ggaggcggtc gaaattctcg tagagcgggt tctccttgcc
     4981 gttgactatg atccactgag caaggaaggc gccgccgcgg ctcacgatgt cggtcactct
     5041 cccctgtttt ctcaggcgct ccagggcgct cttcgttaca ccgcaatgaa ccgtcacgaa
     5101 atcgacccca tcggcgccgt gtttctcaat gacctcgaag atgtcgtccg cttccatcct
     5161 gacgatcgcc tttctcttct ttacggtctc aatggcggcc tggtatatgg gaacggtccc
     5221 cacggggatc ttagcggcat ctatgacgat cttacggatc tcgtcgaggt tgcctccggt
     5281 gctgaggtcc atgaccgcat ctgctcccgc ttcacgggcg acggtcagct tctcgatctc
     5341 ctcgtcgatg ttcaccctct ccggagaggt gcctatgttg gcgttgacct tgacggaaag
     5401 gcccttgcca acacctttcg gggtgaagtc cctgtgtcgg ttgtttgcga gaatcgtggc
     5461 ctttccctcc gcgatgtgct ggcggagatg ttccttgtct aggccctcat cgcgggcgac
     5521 gcgctccata accctggtga tctttccttc ccgtgctgca ataagttggt ttttcatggt
     5581 cgtatcatct ccatattctc aacggacccg ggtcttccgg cggtttcccg tgagcccctt
     5641 gtattttcat gcgttgccct gccgtgtcga aattcctttc cgtcgccgaa gaaacgtaga
     5701 aatgcccttt tctgtcgatc ctgtaactcg attcgaacag cctctcgaga tacccctgcg
     5761 ttgccatgaa ggcatgagac aggggatatc ccgccgcaag aaaacaggcg agggccgaag
     5821 aaaaggaaca gcccgtcccg tggatcgacc ggggaaatct cgccttcctg tagagaaggt
     5881 attccctgcc atcgaacaac acatcaacag ggtcgccctc caggtgacct ccttttatga
     5941 cgacggcacc ggggccaaga gagcgtatct tctcggctgc cttcctggcg gccttcaccg
     6001 tcccgacctt tatacccgtg atggcggacg cctcttccgc attgggcgtt atgaccgtga
     6061 ccttctcgaa aagcacatcg atgcacgctg ctctgccctc atccgtaatg agcgggacac
     6121 cgttcttggc ggcaaacaca gggtcgagaa cgacgggcgc gcccctcctt ttcgccagga
     6181 agctcgctgt cgccctgaca tgggattcgt cgcagagaac gcctatcttc acgcccttta
     6241 ctggaatccc cgccgtggcc gcccgcagga cgtcccggaa gagatcctcc gatgatggct
     6301 cgacgcgctc cacgccgccg gggccctgga ccacagtgca ggtaggcacg gcaattccat
     6361 ggaaaccgaa agagaagaag gtgtcgacat ccctcacgac acccgctccc gaagaggcat
     6421 caaatcctgc tatggaaacg aggtttttca tctttctcgg ctcgtatgta ccccttagat
     6481 taaaactgaa tactatacca tagttagcct cctgaggcca tacaaatccc gcggtaagac
     6541 cgcttgaatc gcttgaatgg ccaaaaggtc aaggcaagga catgatcttc actgttcctg
     6601 gacccgcgaa cgattaaacc acgaggaccg ttgctgcgcc atggtgcagg gcgtgtattc
     6661 gacggttttg tcgaaggctc tctgtgacgt cgacatttgt ttccgtgaaa tcttccggtc
     6721 atcaatggtc ataatacatc tttccctacg tttaccgtga gttacgaaca tcttcagcaa
     6781 atggcacggt aattgccaaa aaggaagcta tgatttgcaa aaagccatat gaggaggtta
     6841 caatgaagaa gattttggga gttatcgtgg cggtcctgtt catagcagcg gcatctactg
     6901 tcttcgccgt cgggccggga ggttacggtc cgggaccaaa agggttcggg tacggccggg
     6961 gtatagatct ctccaaggaa cagatggaca agatgtggca gatacgcgag agattcaaca
     7021 ccgacacctc atccctgcgt tacgagctct ttcagaagcg gggcgaactg aggaccctct
     7081 attccgaccc caaggcaagc gatgcggcga tcctggcaaa ggaaaaggaa gtcgacactc
     7141 tgaggcagaa gatgcacgac aggatggtcc ggttcaaact ggagcagagg aaggtctaca
     7201 ctccggagca gcttcagaaa ctggctgagt ccggttatgg tcccggcttc ggcggcggtc
     7261 gcggcggtcg cggcggcggg cggggactcg ggccctgtgg cagattctag gtacatggaa
     7321 acaaggtgac gggcctcccc ttgacggccc gtcaccccca tgaaaaggga gggagaaatg
     7381 aaaaatatcg tagcagttgt actactggcg acagtgacag ggttgttttt ggtctcatgc
     7441 gcgtctgaag ggtataacac ccagaaaggc gccgcgatcg gggccggact tggcgccata
     7501 gcgggacagg tgatagggca cgatacagcc tcgacgctca tcggcgcggc ggtgggaacc
     7561 cttgtcggag ccgtgggagg caacgcggtg gaccagcacg tgcagaacca gcagcgcact
     7621 tcgactcagc agagcgccgc atatccgtca tcgagccagg taacccccca ggaccagcct
     7681 ccggggcagt gggttgaagt gcccggtcaa tggataggcg ggaaatgggt tcccgcacac
     7741 aaggtatggg tgcccgtgaa tccatgacat ttgtcgtatt cttccttctc ctgtgcgtgt
     7801 tcataacgcc ctctctccgg gcccagaact catattccga gttcgagaga gggctccagc
     7861 tgacggaacc ccagagaacg caggttgagg atatcaggaa caggtatatc aacgaatggc
     7921 aatccctgag gagggaatcg tcgcgaaagc agagggagct cagggaactg aaccggaacc
     7981 ccgagaccaa ttcagccagg ataggcagga tacagggcga actgcaggaa ctcgacaccg
     8041 cgcggcacaa ctcctaccag caataccgct ccgaggtctc gcgaactctc aaccggagac
     8101 aacgcgagca gtacaacagc ttctgcgacc aggagcgcag aaagaacgtg cagcgttttc
     8161 gtccggggcg gcatgggaga taagcgagct gttgcccttc cgctgttctt cgcgcttttt
     8221 ctcgttctca ttacactcac cgggttcttc cagataagga tcatccgcaa caacatcgag
     8281 cggcagctca tgggagaggc agagatcatc ttcacccatg tccagagaga gatagacatc
     8341 aacctcgagt acctcagctt cctggacagg tcacccgtca tcatcacccc ctatttcttc
     8401 aacatcatgg ccttcgacga gtcgatcatc gacgaccttg acgccgtcat gggccggttt
     8461 ctcaagaacc cggcagaaag gggcctggaa gaactctccc tggcgaacgt catcgagtac
     8521 gacctcacgg gtaagatcgt cgccgaaaaa gggagaagcg acattcccgc cggtctcctg
     8581 caaaaaatcg tctcggggga ggaggcgacc gtcatcagga tgcccgtagc cggcgacagg
     8641 tcgctcgtct ttggcaggcg tctgggtgaa cgcatccttt ttgtaaggat caacgccccg
     8701 gaactggaaa cattaagaag aaaggtcatc ctcagggata tcgtcgatag agaggagaag
     8761 aggttcaaca tcgactccat caggatctat gacgagaaag gcggtctctt cgtcgggacc
     8821 agcgatgccg ggaagaacac tttccttctc gagcgggatc tttcctcgaa ggtcctcccc
     8881 ggctttcgca tgcagatctt tatctcgaga aaccttgcac gggacaccat ccagaggtcg
     8941 gccatcggct tcatactcat ccttgcggtc ctcttcgtct ccggagccgg gagcatctac
     9001 gccttcttcg tcatggagag gaagtacgcg aaaagggtca aggatatgga gcgggagatg
     9061 gagatcaagg aacgcctggt atcgctggga aaactggctt ccgggatggc ccacgagatc
     9121 aggaatcccc tgaacgccat cagcctttcc gttcagcgcc tcaagagaga attcgtccct
     9181 tgggacgaga agaaggaaga atacctcacc ttcctcgata tcatcaggaa ggagctcacg
     9241 cgggtcaatg ggatagtcga ggagttcctc caatcgacca gggcgcaggc ccccttcagc
     9301 agggaagacc tgcgcgacct tcttgaagag gtgatcataa tcgtcggcga aaaggcctca
     9361 tcgaagggta tcttcatcga gaacgccaca ggcaccggca tccacgtcga ttgccagagg
     9421 gacaggctta agcaggcatt ctacaacatc atcataaatg ccatagagtc catgaaggac
     9481 ggcggaaaga tcgccatcac ggtgaagcag agagactccc gcgtcgaaat atcggtcagg
     9541 gattccgggc ccggcatcag tgaagaggcg ctggcgagga tattcgaata ctactacacc
     9601 acaaaggaca agggtatggg cctcggactg cccatatcgt acgtgatagt gaaagaccat
     9661 ggcggcgaca tcaaggtgac aagcgctgcc gggcagggaa ccaccttcgt catcgtcctg
     9721 ccccgcacgc aagacgccac gacggcggcg taaagggaaa ggaggggttg cggttatgga
     9781 cagggtcggc atattgatcg tggaagacga acagacacag agatcgctcc tcggcgggct
     9841 gctcaggaaa gaaggctaca cgataggtga ggcatccgac ggagaaagcg ccctcgctct
     9901 ttttcaaaaa gacattttcg agatcgtgct tctcgactac agactgccgg acacggacgg
     9961 cctgtccctc ctcaaaagga taaaggagat aaacccggcg acggaggtgg tcatggtcac
    10021 tgccttcggg agcatagaga acgccgtcgg ggccctcaag gccggggcgt ccgagtacct
    10081 tacgaaaccc atcgacctcg acgaccttct cttcaagctg cgcaaggtcg aggaaaagac
    10141 ataccttatc cgcgagaaca tggtccttcg tgaaaccctg cgggaccgct tcaagtcgga
    10201 ggagttcgtt taccagagcg agaggatgca cgaggtctcg agcctggtgg tcaggatagc
    10261 gaagacggat tcaacgtgca ttataagcgg cgagagcggt gttggaaagg aggtcgtcct
    10321 caacatgatc cacgcgctca gcgagcgcaa ggatttcccc ctcgtcaagg tcaattgcgc
    10381 cgccattccc gagacgctcc tggagagtga actcttcggc tatgaaaagg gcgccttcac
    10441 cggcgcttac cagcgcaagg ccggcaagtt cgaactcgcc aacaaaggga ccatctttct
    10501 tgacgagata ggcgacatcc ccctcgtgct tcaatcaaag ctcctcaggg tgatccagga
    10561 gaaagaggtc gagcgcctcg gaggggcgca tcccgtcaag atcgatgtcc gcatcgtcgc
    10621 cgcgaccaat cgcaacctgg aggaggaggt caaaaaggga acattccggg aagacctgta
    10681 ctatcgcctc aatgtcgtca atatcgtcat ccctccctta agggagcgca gggaggacat
    10741 tccgctgctc atcgattttt tcctgaagaa atacaacctc aagcataaga agaacgtcaa
    10801 gggtctcacg cgggaggcac gcgacaccat ggtcaaatac gactacccgg gcaacgtcag
    10861 ggaactggag aacatcgtgg agagggccat agtccttacc cggggagacc acatcaccag
    10921 ccaggacctt ccgaacctgg cggagcaggc aatggtgaca ggcgacggca gtattcgggg
    10981 aaccgtcgaa tccatcgaga gatcgatgat cattgaagcc ctcattaatg ccgactgggt
    11041 ccagacgaag gccgcgatgg ccctcggtct gtcggagcgc atgctgaggt ataagatcaa
    11101 gaagtacggc atatcgcgat catcccgctc cgcgggctga tcgcgcaaac gggcccacgg
    11161 gtcggcgctc gcgcacctga cgggcatacg cggaaaagca aaaggacccc cagggaaaaa
    11221 gtctgcggtc agcggattcc gaccgtcccc gcccttacct cgttagcccg tttgcgctta
    11281 gcgcgcagac ccgtccgccc gtcaggcacg ttggtgccag cccgtatgcg cgtggggtgg
    11341 acgctttagc attcatattt tcccttccgt atgttataga taaa
//
LOCUS       DAOPOA010000004        11040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_6781_length_11040_cov_5.664816, whole genome shotgun sequence.
ACCESSION   DAOPOA010000004 DAOPOA010000000
VERSION     DAOPOA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6781_length_11040_cov_5.664816"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1697)
                     /locus_tag="PKJ17_00155"
     CDS             complement(126..1697)
                     /locus_tag="PKJ17_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74402.1"
                     /translation="MNPITYLDFQKPSTEMAIWGSIGVGLMLLISIILGFMSKRKVAK
                     GSFEEYLVGKRDIGPVVTGCALAASYLSGWAFAGSTGVVYAVGFSGMWFAGIWSLLGL
                     IPCCWLAAVKTASFSKKLGAATLPETIGRRFESKMLQTVVAICMLYFLFMYSVGQLKA
                     AGGVWYATTGLPAVWCLLLSIFIAWLYMVLGGYVGTQWSMAFQGAFLGFVGALLGIWA
                     IIWAGGFYEISAKLYMEPKVGPALIKLIRPELPKVGPTQLFSSMVGILATPVIFFTMA
                     IGFPHNVSRFLGMGTLNKKGYWTLIIMVYLIAGVPIMLDCSSNGLVARMIYGPNLLKV
                     KPWAADLAAPELAFAVGGVPMMTLYVMGLFAAALSTLAGMVFIMSANITRDVIKLWWP
                     QVSDKSMLYLGYFLIALFLFLPFYWTLVNPPPLLSIFMGLAAMGLGAIFVFVTAASYY
                     WKGATKWGALCCVLYGTFGSMYGGYKVLFKKEVGLGTFEWWLVIGCGILYFVVSAITK
                     KPSQELLDKLFPAKS"
     gene            complement(2182..5517)
                     /locus_tag="PKJ17_00160"
     CDS             complement(2182..5517)
                     /locus_tag="PKJ17_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD74403.1"
                     /translation="MKLARLEIFGFKSFLNRTVFQFGEGITSIVGPNGCGKSNIVDAI
                     VWVLGERGTKSLRVKDMGDVIFHGSNGKRPVNIAEVTLDLTEGDREFVVKRRIYRDGT
                     NEYSLNGKIVRLKDVQDAFLGTGLGTNSYAIIEQGRIEALISMKPAERRVVIEEASGI
                     TRFEEKKRDAVNRMEETTSNLERIEDIYREVTASFGKAEQEWERWKAYQELADRLHEI
                     DRQLLLDGYAKLRKRMTKVRERQADLEEEILQKEADRSTVREEFEAKESEFALTDNII
                     RQLEVDLKSKEKDMESRLLEIEYLGEEVKRLEASRKGLAEEESSIAAETARHEKEIDA
                     LKERSGALAAELEEEKGKEARTRDVAQGFKTNIEEHEKILEESRTKLFVSMSALTETR
                     NRIAQIERQAEERRKRMERQAAEVKELEMLLASLDAKRKALHDTLQEALREKESLSAR
                     ESAAAGEREGILEEMERQKALVESARNEKRVKEEFLRQLGGYIEGPEDAVRAEDRLAN
                     LIRTEESRIKALERFFSEELEYEVVKSDSVEALSLAVREREGNRVFFPPKGIFRKAGD
                     DVEMEVAWIADIGEALSRVAAGEEGIFINDDVYVDSRGIILTGGASKKIDVRQFREKL
                     KLEKELQTLSADVERHGSALAASRPRLEACDAKCRSARAAREAQEGKARALEKDLLMA
                     EAQWKSAADRLTALQAATESPDDAGPEVLESLVADRGRREEEKAAIEASMARLRETLA
                     GLKKEHEAAQSAWHAVTIEIERKMNMLKSISDNRERSEAAIRSLADKTQGLKEKYAAI
                     DRDIEGRTQKIRYLERSYEELQADLQKHVARFEELKTMLGNLHAQKQTLQESIDTLGK
                     EIERVRARKENAEKDLAVLEEKRLTIVEHLVGTYGIDQPDEMSVQLPADIEEERESLT
                     ARIAEMGEINFRAEKEYLELKERISFLDKQKEDLLTAMESLRKTITKIDQFTKELFTE
                     TFDKVGSAFSRFTDLLFKGGKGHLAINAEQGGVDLFVQPPGKKVTRMELLSGGEKALI
                     SLAFLLALMDTRPSPFALMDEIDAPLDDANLAGLLDIIRGMSLKSQIVFITHNRITME
                     YSDMIYGITMEEQGISKTVSVRL"
     gene            complement(5522..6925)
                     /gene="gltX"
                     /locus_tag="PKJ17_00165"
     CDS             complement(5522..6925)
                     /gene="gltX"
                     /locus_tag="PKJ17_00165"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOD74404.1"
                     /translation="MVKSRFAPSPTGNLHIGGARTALFNYLYTKHHGGVFVLRIEDTD
                     VERSKDDYVKDILGGLSWLGIHWDEGPYYQMDRMDLYREHAYRLLAEGHAYKCYCTAE
                     MLEEKRKKALKEGGKPHYDRTCRDLLPDDASSEGKPFVIRFRSPVSGEVSFTDLIRGT
                     ITFKCEELDDLIIFRTDSTPTYNFTVVVDDALMGITHIVRGDDHINNTPRQILIYEAL
                     KYPVPQFAHVPLIHGKDRTRLSKRHGATSLLEYRSDGFLPEALMNYLARLGWAHGDQE
                     IFSQQELIEKFDLPNVGKSPSVFDMDKLTWLNGHYLKTLPAEDIAGRLLPFLQRMGIE
                     ASPDGRLVRIVVNLRERARTLKEMAQMAEYFFRDDFAFDETARVKFLTAATRPILVDF
                     AEGLKAIASTDEEESLKALIEGLTKKYNAKPVGIIQPVRVALCGRTVSPGIFEVIAIL
                     GKEAVEKRLTRAVESIQ"
     gene            7074..7679
                     /locus_tag="PKJ17_00170"
     CDS             7074..7679
                     /locus_tag="PKJ17_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOD74405.1"
                     /translation="MEKRFSNELITVIEKGDLESVVQFISKGADVNNADQNGNTPLMY
                     ASMRGFADTARFLLDNGADVNARNAQGVTPLTYACRNNHVGVLRLLVSRGADVNVPDR
                     DGGTALTFASKSGNLEIVRLLVEHGADLNFRDHDGDTPLMCAINGKFAGIINFLISRG
                     ADLAVMNKHNKTALDLASEKGLLRIVNDIKFKLLERQKQVS"
     gene            7836..8363
                     /locus_tag="PKJ17_00175"
     CDS             7836..8363
                     /locus_tag="PKJ17_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOD74406.1"
                     /translation="MFDVGEVAVYPGHGVGRIESIEEKEFSGTKQSFYIIRILDTDMT
                     IMIPIDGAKNAGLRSVIDACEVCKVYDILKDKNVVHDNSPWNRRYKEYMERIKSGSIY
                     EVATVLKELYNLRYWKELSFGEKKMFEMARNLITKELSMSLGTEELIVEQEIEEIFLA
                     NNNSQKGNTANHHKG"
     gene            8368..9411
                     /locus_tag="PKJ17_00180"
     CDS             8368..9411
                     /locus_tag="PKJ17_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOD74407.1"
                     /translation="MDIFIQIILLTVTTLTGYFILKELLSSGVWGLVGATCGLILGYA
                     ILKIELKLRDISLKIILGSLTGVVVSLFVANLFVSRLLLALIKDIPITLPIYILFYFI
                     MGYLGFVLGKEKSKTIDLSKVPLFDRLEEDKDIKLLDTSTIIDGRIADICETGFVQGT
                     LVIPQFVLYEIQHIADHQDSVKRTRGRRGLDVLHRLQKQTFLTVRIVDYDFPKLKDVD
                     TKLIALAKRLGGKIVTNDYNLNKVAELQGIEVLNMNQLAMSLRPALLPGEQMNIKVLR
                     EGKEQGQGIGYLDDGTMVVVDDARKYLGKAVDVVVTSVLQTTSGRMIFSRLKGEAGKD
                     YIYPDEYQLEEQA"
     gene            9419..10117
                     /gene="ispD"
                     /locus_tag="PKJ17_00185"
     CDS             9419..10117
                     /gene="ispD"
                     /locus_tag="PKJ17_00185"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOD74408.1"
                     /translation="MRTIAIILAGGAGKRMGAATNKQFLLIENKPIIVHTLQIFEDCR
                     AVDGIYFVVNQKDLPLIQEEILETYKFNKIMKIVMGGKLRQDSVRNGLEAIEHPCDIV
                     VIHDGARPFVSPAFIEKGIFLMEMYDAVIPALPVKDTVKVISKEGFVVKTLERDSLWH
                     VQTPQTFKYRLIMKAYREGMAKRHYGYDDATFIEHLGKKVKVIEGSPYNMKITTPEDL
                     TIARGLLSQLKGTL"
     gene            10114..10587
                     /gene="ispF"
                     /locus_tag="PKJ17_00190"
     CDS             10114..10587
                     /gene="ispF"
                     /locus_tag="PKJ17_00190"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOD74409.1"
                     /translation="MRIGTGFDVHRLTDGRRLVLGGVEVPFGKGLLGHSDGDVLLHAI
                     SDAILGALSEGDIGVHFPDTDKEIEGIDSAVILARICAIARDRGYRVVNVDSVVVAQA
                     PKIAPYREAMRERIAGLLSIDAGMVGLKGKTTEGLGLTGRGEGIAAHAVVLMDRV"
     gene            10630..>11040
                     /locus_tag="PKJ17_00195"
     CDS             10630..>11040
                     /locus_tag="PKJ17_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOD74410.1"
                     /translation="MVRVRFAPSPTGHLHVGNAKTALFNWFFARKNGGSFVLRMEDTD
                     VERSDAAYERSITDDLAWLGIAWDEGPLRQSERLDVYRSHAMVLLEKGLAYRCFCSEQ
                     ELAAARQRSLARGEPPRYDGKCRDLSAGEAVRLTD"
BASE COUNT         2260 a         3205 c         3163 g         2412 t
ORIGIN      
        1 cgcgacggtg tatgtttcca tctcccctcc gttttcgttt gtccgatcgt cccgatcctg
       61 tgccttgcag gttgaaagaa aagaggggct tgtgaaagcc cctctcgatg ttgtttcgac
      121 cgatatcagc tcttcgccgg aaacagcttg tcgagcagtt cctgggaggg ctttttcgtg
      181 atggcgctga cgacgaagta cagtatgccg cagccgataa cgagccacca ctcgaaggtc
      241 cccaagccga cctctttctt gaagaggacc ttgtagccgc cgtacatgct cccaaacgtg
      301 ccgtaaagaa cgcagcacag cgcgccccac ttcgtagcac ccttccagta gtaggaggca
      361 gcggtcacga agacgaagat ggcgcctaga cccatggccg cgaggcccat gaagatggac
      421 aagagcggcg gcgggttgac gagcgtccag tagaagggca ggaaaaggaa gagggcgatg
      481 aggaagtagc ccaggtacag catggacttg tccgatacct gcggccacca cagtttgatg
      541 acgtcccgtg tgatgttggc gctcatgatg aagaccatgc cggcgagcgt ggagagtgcc
      601 gcagcgaaaa gacccatgac ataaagtgtc atcatgggaa caccgccgac ggcaaaggct
      661 agctcaggcg ccgcgaggtc cgccgcccag ggtttgacct tgaggaggtt cggtccgtag
      721 atcatcctgg ccacgagtcc gttgctcgag cagtcgagca tgatggggac gccggcgatg
      781 aggtagacca tgatgatcaa tgtccagtaa cccttcttgt tgagggttcc catgccgagg
      841 aaccggctca cgttatgcgg gaagccgatg gccatggtga agaagatgac cggcgtggcc
      901 agtatgccta ccatgctcga gaagagctgt gtcgggccca ccttgggaag ttcaggccgt
      961 atgagcttga tgagcgcggg gcccaccttc ggttccatgt aaagcttcgc ggatatttca
     1021 tagaagccgc cggcccagat gatcgcccat atgccgagga gcgccccgac aaagccgagg
     1081 aaggcgccct ggaaggccat ggaccactgg gtgccgacat agccgccgag gaccatgtag
     1141 agccaggcga tgaagatgga caggagcaaa caccataccg cgggaaggcc cgtcgtggcg
     1201 taccatacgc cgcctgccgc cttgagctgg cccaccgaat acatgaagag gaagtagagc
     1261 atgcagatgg cgacgacggt ctggagcatc ttgctctcaa accttcggcc gatcgtctca
     1321 ggcagcgtgg ccgcgccgag cttcttcgaa aaggaggccg tcttgacggc ggcgagccag
     1381 cagcacggga tgaggccgag aaggctccag atgcccgcga accacatgcc cgagaaaccg
     1441 acggcatata cgacgcccgt gcttccggcg aacgcccagc cggacaggta ggatgccgcc
     1501 agggcgcaac cggtaacgac cggaccgata tcccgtttgc cgacaagata ttcttcgaag
     1561 gatcccttcg ccacctttct cttgctcata aatccgagga tgatggagat gaggagcatg
     1621 agcccaacgc cgatgctgcc ccatatcgcc atttccgttg atggtttctg aaagtcaagg
     1681 tacgttatcg gattcattcc ttccaccccc ttgttatgag ccaggtgccg ataaaggtca
     1741 tgagaaagag tccgatgatg agaacccacc acacatacca ggatgccatc aatactgatg
     1801 catcgggaag tgtgacggga ggtactgctg gtactgttgg ctgcataata accccctttg
     1861 cgtttttaaa aacacatttc ccctactgaa ggtaagtccc acctcctttc agcaaactgt
     1921 tatcaaaaaa agaggacacg tgaaaaaatg aaaccgttgt tgtcgctaca acggtatgga
     1981 tatagtgata gacttgatat tccaaacgcc cccttcccct ttataataat aaatcatagt
     2041 gttctaagga taaatgtcaa gaaaaaaatc ccttccccga cgcgcgtcaa aagcgaccag
     2101 aaaaggaagt atacagtata tcaacgggat gtggcgacat tgtacaaaaa agcatttgca
     2161 aggggatagg gctttgccgc cctagagcct tacggacacc gttttggata tgccctgctc
     2221 ttccatggtg ataccgtata tcatgtccga atactccatg gttatcctgt tgtgggtgat
     2281 gaagacgatc tgggacttga ggctcatccc ccgtatgatg tcgagaaggc ccgcgaggtt
     2341 cgcgtcgtcg agcggcgcgt ctatctcgtc catcagcgcg aaggggcttg gcctcgtgtc
     2401 catgagggca aggaggaacg ccagcgatat gagggccttc tcgccgccgg agagaagctc
     2461 catcctggtc acctttttcc cgggaggctg gacgaagaga tcgacgccgc cctgctcggc
     2521 gttgatcgcg aggtggccct tgccgccctt gaagagaagg tcggtgaaac gcgagaaggc
     2581 gctgcccacc ttgtcgaagg tttccgtgaa gagctccttc gtaaactggt cgatcttcgt
     2641 tatcgtcttc ctcagggatt ccatcgcggt gaggaggtct tccttctgct tgtccaggaa
     2701 ggatatcctc tctttcagtt caaggtattc cttctcggcc ctgaagttga tctcacccat
     2761 ttcggctatc ctcgcggtga gcgattcgcg ctcctcttct atatctgccg gcagctgcac
     2821 cgacatttcg tccggctggt cgatgccgta tgtgccgacg aggtgctcga cgatggtgag
     2881 gcgtttctcc tcgaggacgg cgaggtcttt ttcggcgttc tccttcctgg cccggacccg
     2941 ttcgatctcc ttgccaaggg tgtcgatgga ttcctggagc gtctgtttct gggcatgaag
     3001 gttgccgagc atcgtcttga gctcttcgaa tctcgcgacg tgtttctgca ggtccgcctg
     3061 gagttcctcg tatgaccgct ccagataccg tatcttctgc gtgcgtccct cgatgtccct
     3121 gtcgatcgcc gcgtattttt ccttgagccc ctgtgtcttg tctgcaagcg accgtatggc
     3181 tgcctcggag cgctcccggt tgtcgcttat ggacttgagc atgttcatct ttctttctat
     3241 ctcaatggtg acggcgtgcc atgccgactg cgccgcctca tgctccttct tcagtcctgc
     3301 cagcgtttcc ctcaatcgtg ccatggaggc ctctatcgcg gccttttcct cttcccggcg
     3361 gccgcgatcc gcgacaagcg attccagcac ctcggggccg gcatcgtcgg gagactctgt
     3421 tgctgcttga agggccgtga gcctgtcggc ggcgcttttc cactgggctt ccgccatgag
     3481 aaggtccttc tcgagcgccc ttgcctttcc ttcctgtgcc tcacgggccg cgcgggcgga
     3541 acggcacttt gcgtcacagg cctcgagacg aggccttgag gccgcaaggg cactgccgtg
     3601 gcgttccaca tcggctgaca gggtctgcag ttccttctcg agcttcagtt tctccctgaa
     3661 ctgcctcacg tcgatcttct tcgacgcccc tccggtaagg atgatgccgc gggagtcgac
     3721 atagacatcg tcattgatga atatcccttc ttcgccggca gcgacccgcg agagagcctc
     3781 tccgatatcc gctatccagg cgacctccat ctcgacatcg tccccggcct tcctgaatat
     3841 ccctttcgga ggaaagaaca cacggttgcc ttcccgctcc cgcacggcta aggacagggc
     3901 ctccacggaa tcgctcttca cgacctcgta ttcgagctct tccgagaaga acctttcaag
     3961 cgccttgatc ctcgattctt ccgtcctgat caggttggcg aggcggtctt ccgcgcgtac
     4021 ggcgtcctcg gggccctcga tgtagccgcc gagctgtctg aggaactctt ccttgaccct
     4081 cttctcgttc cgcgcgctct cgacgagagc cttctgcctc tccatttcct ccaggatgcc
     4141 ctcgcgctcg cccgcggcgg ccgattccct tgcggacagg gattcttttt ctctgagcgc
     4201 ctcctgcagc gtgtcgtgaa gggccttacg cttcgcgtcg agggatgcga ggagcatttc
     4261 gagttccttc acctcggctg cctggcgttc catacgcttc cgcctctcct cggcctgccg
     4321 ctcgatctgt gctatcctgt tgcgagtctc cgtgagggcg ctcatggaga cgaagagctt
     4381 agtacgggac tcttccagga tcttctcgtg ctcctcgata tttgtcttga agccctgcgc
     4441 tacatcccgg gtgcgcgcct ctttgccctt ttcctcttcc agctcggccg ccagggcccc
     4501 gcttctttcc ttgagggcat cgatctcttt ctcatggcgc gccgtctccg cggcgatgga
     4561 ggactcctct tccgcgagac ctttgcggga cgcctcgaga cgtttcacct cttcgcccag
     4621 gtactctatc tcgagaaggc ggctctccat gtccttttcc ttgctcttca ggtcgacctc
     4681 aagctggcgg atgatgttgt cggtgagggc gaactcgctc tccttcgctt cgaactcctc
     4741 tctgactgtc gacctgtccg cttccttctg gagtatctct tcctcgaggt ctgcctgccg
     4801 ctcgcgcacc tttgtcatgc gctttcggag cttcgcgtag ccgtcaagaa ggagctgcct
     4861 gtctatctcg tggagcctgt cggccagttc ctggtatgcc ttccagcgtt cccactcctg
     4921 ctccgccttg ccgaacgaag cggtgacctc acggtatatg tcctcgatgc gttccaggtt
     4981 cgacgtcgtt tcttccatgc ggttcacggc gtcccgcttc ttctcctcga accgcgttat
     5041 gccgctcgcc tcttcgataa cgacgcgccg ctcggcgggt ttcatgctga tcagggcctc
     5101 gatgcgcccc tgctcgatga tggcgtagga attcgtgccc aggcccgttc ccaggaaggc
     5161 gtcctgcacg tccttcaagc ggacgatctt gccgttgagg gaatattcgt tggtgccgtc
     5221 ccggtatatc cgtcttttga cgacgaactc cctgtctccc tccgtgagat ccagggtcac
     5281 ctcggcgatg ttgacgggtc tcttcccgtt gctgccgtga aagatgacgt cgcccatgtc
     5341 cttgaccctc aggctctttg tgcccctctc gccaaggacc cagactatgg cgtccactat
     5401 attgctcttt ccgcagccgt tgggcccgac gatggacgtg atgccttcgc cgaactggaa
     5461 gacggtgcgg ttaagaaagg atttgaaacc gaagatttcg agtctggcca gtttcattgg
     5521 ttcactggat cgattcgacg gcccgcgtca atcttttctc cacggcctct ttgcccagga
     5581 tggcgatgac ctcgaatatg ccggggctta cggtcctgcc gcaaagcgcc actctgaccg
     5641 gttggatgat cccgacgggc ttggcattgt actttttcgt caatccctcg atgagcgcct
     5701 tgaggctctc ctcttcatcc gtggaggcga tggccttgag cccttcggcg aaatcgacga
     5761 gaatgggtct cgtcgcggcg gtgaggaact tcacgcgggc ggtctcgtcg aaggcaaagt
     5821 cgtcgcggaa aaagtactcc gccatctgcg ccatctcctt cagggttcgc gcgcgctcgc
     5881 ggaggttgac cacgatcctg acaagtcggc cgtccgggga ggcctcgatc cccatgcgtt
     5941 ggagaaaggg caggagcctc cctgcgatat cctccgcggg aagggtcttc aggtagtggc
     6001 cgttgagcca ggtcagcttg tccatatcaa agacggaagg cgacttcccc acgttgggaa
     6061 ggtcgaactt ctcgatcagt tcctgctggc tgaatatttc ctggtcgccg tgcgcccagc
     6121 cgagacgtgc cagatagttc atgagggcct cggggaggaa gccgtcgctg cggtattcca
     6181 ggagagacgt ggcgccgtgg cgtttggaca gtcgggtcct gtcctttccg tgaatgaggg
     6241 gcacgtgggc gaactgcggg acggggtatt tcagcgcctc gtagatgagt atctgccgcg
     6301 gggtgttgtt gatgtggtcg tcgccgcgga cgatgtgggt gatgcccatc agcgcgtcgt
     6361 cgacgacgac ggtgaagttg tacgtgggcg tcgagtcggt ccggaagatg atcaggtcgt
     6421 cgagttcctc gcatttgaag gttatcgtgc cgcggatgag gtcggtgaag ctcacctcgc
     6481 cgcttacggg gctgcggaag cggatgacga aaggcttgcc ttcactggag gcgtcatcgg
     6541 ggagaaggtc acggcatgtc ctgtcatagt ggggcttgcc gccctccttg agcgccttct
     6601 ttctcttttc ctcgagcatc tccgcggtgc agtaacactt gtacgcgtgt ccttccgcca
     6661 gcagtctgta ggcgtgttcc cggtagaggt ccatacggtc catctggtag tacggcccct
     6721 cgtcccagtg gataccgagc caggacagtc cgccgagtat gtctttcacg tagtcgtcct
     6781 ttgaccgttc gacatccgtg tcctctatcc tcaggacaaa gacccctcca tgatgcttcg
     6841 tgtaaagata attgaaaaga gcggtccggg cgccgcctat gtggaggttc cccgttggac
     6901 tcggcgcgaa ccttgacttg accattattg tgtcctccag ataaagattg tagtgattat
     6961 aaaggaaaaa tcccccggca tcaatccatt tttcagaaga cgcgccggca ccttccctgg
     7021 cagctcctca aaaaaacccc ccgttttttt ggaggtggta tataatgatc taggtggaga
     7081 aacgattctc aaatgaattg atcaccgtca tcgaaaaggg cgacctcgag agtgttgtcc
     7141 agtttatcag caaaggcgcc gatgtcaaca atgcggatca gaacggcaac actcctctca
     7201 tgtatgcgtc gatgcgcgga ttcgcggaca cggcgcggtt tcttctcgac aacggggcgg
     7261 acgtcaatgc ccggaacgcg cagggtgtga ccccgctgac gtatgcttgc aggaacaacc
     7321 acgtgggcgt cctgaggctc cttgtctccc gtggggccga cgtcaacgtt cccgacaggg
     7381 atggcggcac ggccctcact tttgccagca agagcggcaa cctggagatc gtcaggctcc
     7441 tggtggagca cggcgcggac ctcaatttcc gggaccacga cggcgataca ccgctcatgt
     7501 gcgccataaa cggcaaattc gccggcatca taaactttct catcagccgg ggcgcagacc
     7561 tggccgtaat gaacaagcac aacaagaccg ccctcgacct cgcctccgaa aaggggctcc
     7621 tcaggatcgt caacgacata aagttcaagc ttctggaaag gcagaagcag gtgtcctgag
     7681 ccccgtttac cgcttctctc ccgagagaga ggggcgaagc cttggattac ctgccgatta
     7741 ccacataata accattactt atttaatttt tctcaatatt atgctacaat atggcaacct
     7801 ggccgaaaaa cgatgaaaaa ctattcggag gcataatgtt tgatgtagga gaggttgcag
     7861 tatatccggg gcatggtgtc ggtcggatcg agtctatcga agaaaaagag ttctcaggta
     7921 ccaaacagtc attttacata atccgcatcc ttgatacgga catgaccatc atgataccta
     7981 tcgatggcgc gaagaatgcc ggcttgagaa gcgtcatcga tgcctgcgag gtgtgcaagg
     8041 tctacgatat tctcaaggac aagaacgttg ttcacgacaa ctcgccctgg aacaggcggt
     8101 acaaggaata catggagagg atcaagagcg gctccatcta tgaggtcgcg acggtcctga
     8161 aagagctcta caacctccgg tactggaaag agctttcctt tggcgaaaaa aagatgttcg
     8221 agatggcgcg caatctcatc accaaggagc tttccatgtc tctgggcacc gaggagctca
     8281 tagtggagca ggagatcgaa gagatcttcc tggccaacaa caacagtcaa aaaggaaata
     8341 ccgccaatca ccacaagggt tgagtccttg gatatattca ttcagataat cctcctgacc
     8401 gtcacaaccc ttaccggata tttcatactt aaagaacttc tttcgagcgg agtctggggc
     8461 ctcgtcgggg cgacgtgcgg gctcatcctc gggtatgcca tactgaagat cgagctgaag
     8521 cttcgggaca tatccctgaa gatcattctc ggaagtctca ccggggttgt cgtgagcctt
     8581 ttcgtggcaa accttttcgt ctcccgtctc cttctcgccc tgatcaagga catccccatc
     8641 acgcttccca tttacattct cttctacttc atcatgggct acctcgggtt tgtcctcggg
     8701 aaggagaaat cgaagacgat cgacctctcc aaggttcctc tgttcgaccg tctggaagag
     8761 gacaaggaca tcaagctcct cgatacgagc accatcatag acgggcggat agcggacata
     8821 tgcgagaccg gcttcgtgca ggggaccctc gtcattcccc agttcgtcct ctacgagata
     8881 cagcacatcg ccgaccacca ggactccgtg aaaaggacgc gcggccgccg gggactcgat
     8941 gtcctgcaca ggctgcagaa gcagaccttc ctcaccgtca ggatcgtgga ctacgatttc
     9001 cccaagctga aggacgtcga cacgaagctt atagccctcg cgaaaaggct gggagggaag
     9061 atcgtcacca acgattacaa cctcaacaag gttgccgagc tgcaaggcat cgaggtcctc
     9121 aacatgaacc agcttgccat gtcgctccgg cccgcgctgc tgccgggcga gcagatgaac
     9181 atcaaggtgc tccgcgaagg caaggagcag ggccagggga tcggatacct cgacgacggg
     9241 acgatggtgg tggtcgacga tgccaggaaa tacctgggca aggccgtcga cgtcgtggtc
     9301 acgagcgtcc tgcagacgac ctcggggcgc atgatctttt cgaggctcaa gggagaggcg
     9361 gggaaggatt acatctaccc cgacgaatat caattggaag aacaggcgtg ataccgacat
     9421 gagaacaatc gcaatcattc tcgcaggcgg cgcgggaaag aggatgggtg ccgccaccaa
     9481 caagcagttc ctgctcatcg agaacaagcc catcatcgtt cacacactcc agatatttga
     9541 agactgccgg gccgtggacg ggatctattt tgtcgtcaac cagaaagacc tccctctcat
     9601 ccaggaggag atactggaga cctacaagtt caacaagatc atgaagatcg tcatgggcgg
     9661 caagctcagg caggactcgg tgaggaacgg actggaggcc atcgagcacc cctgcgacat
     9721 agtcgtcata catgacggcg cgcggccttt cgtatccccc gcgttcattg aaaaaggcat
     9781 ctttctcatg gaaatgtacg atgcggtcat accggccctg cccgtgaagg acaccgtgaa
     9841 ggtcatctcc aaggaagggt tcgtcgtgaa gaccctcgag cgggactcgc tctggcacgt
     9901 gcagaccccc cagacgttca aataccgcct catcatgaag gcttaccggg aggggatggc
     9961 gaaaagacac tacggatatg acgacgccac gttcatcgag cacctgggaa agaaggtgaa
    10021 ggtcatagag ggttccccct acaatatgaa gatcaccaca cccgaagacc tgaccatcgc
    10081 gagggggctc ctttcacagc tcaaagggac cttatgagga tagggacagg gttcgacgtc
    10141 catcggctga cggatgggag acggcttgtc ctcgggggtg tcgaggttcc attcgggaag
    10201 ggccttctcg ggcactccga cggcgacgtc ctcctccacg ccataagcga cgcgatactt
    10261 ggcgcactct ctgaaggaga tatcggggtc cacttccccg acaccgacaa ggagatcgag
    10321 ggcatcgaca gcgccgtgat acttgccagg atctgcgcga tcgcccgcga ccggggctac
    10381 cgggtcgtca acgtcgacag cgtcgtcgtg gcacaggcgc cgaagatagc cccgtaccgg
    10441 gaggcgatga gggagaggat cgccggtctc ctgtccattg acgccggcat ggtgggtctc
    10501 aagggcaaga cgaccgaggg cctggggctg accggcaggg gcgaaggtat cgcggcgcac
    10561 gccgtcgttc tcatggacag ggtctgaaag aggcatgtgt cccggatctc cacgacagat
    10621 gaggcgaaaa tggtcagggt aaggtttgcc ccgagcccga cgggacatct ccacgtggga
    10681 aacgcgaaga cggccctctt caactggttc ttcgccagga agaacggggg gtccttcgtt
    10741 ctgcgcatgg aggatacgga cgtcgaacgc tccgacgcgg cctacgagag gtccatcacg
    10801 gatgatcttg cctggctcgg gatcgcctgg gacgaaggac cgctgcgcca gtccgagcgt
    10861 cttgacgtct acaggtccca cgcgatggtc ctcctggaga agggcctcgc gtaccggtgt
    10921 ttctgcagcg aacaggagct tgccgcggcg agacagaggt cgctggcgcg gggcgagcct
    10981 ccgagatatg acggaaaatg ccgggacctt tccgccgggg aggccgtaag gctcacggac
//
LOCUS       DAOPOA010000005        10241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_7449_length_10241_cov_3.616238, whole genome shotgun sequence.
ACCESSION   DAOPOA010000005 DAOPOA010000000
VERSION     DAOPOA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7449_length_10241_cov_3.616238"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..748
                     /locus_tag="PKJ17_00200"
     CDS             17..748
                     /locus_tag="PKJ17_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD74411.1"
                     /translation="MDLLKVENLSKSFGALWAVNNVSFSVAEGEIVGLIGPNGSGKST
                     VFHLITGLLKANKGRISYQGKDITRFKPYQVSRSGIARTFQIVRPFPEMTTLKNVMVG
                     RAFGSSSARSMHDTEDDARRLLELTGLADKVSATAGHLGLVYRKKLELARALATKPRL
                     LLLDEVMAGLNPTEMMETVEFLKGLRNTGVTMIVVEHVIKALFSMTDRVVVLNAGEKI
                     AEGTPHEISRNEEVIKVYLGDYEHA"
     gene            741..1457
                     /locus_tag="PKJ17_00205"
     CDS             741..1457
                     /locus_tag="PKJ17_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD74412.1"
                     /translation="MLEITGIDVFHGDLQAVWDVSLSIEGKGITTLIGSNGSGKSTVL
                     STIAGLLKPVRGSISFDGMRIDTLPAHKIVDAGIAMVPEGRRLFREMTVMDNLEMGAI
                     TKRARKMKNETMAWIFDVFPILKERARQRADTLSGGEQQMLAIGRSLMSQPRLLLVDE
                     LSLGLAPVVVQRISQTLDEINKTKGIGIFLVEQNVVMALELAETGHIIENGRIVGTGK
                     ASELLASDQVRQAYLGMEGE"
     gene            1474..2349
                     /locus_tag="PKJ17_00210"
     CDS             1474..2349
                     /locus_tag="PKJ17_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD74413.1"
                     /translation="MEFAQYITYGVINGSLYALVAIGLALIMGIMGIFNIAQGSLAMI
                     AAYGSVLFFQHMKIDPFVSLALTLPAMFLIGVFMYLTMFMSMNKFDAGQKLRNSMLVS
                     FGLVLIMETLATILWTANERTVTTGYSGTVMALGDLRIPYTGLATIVMAIVVIFGLHS
                     FLKHTTFGKSIWAVSQDHDCAMLMGINVRRTTLAAFGIGVALSAFAGAVVSLHTVSPA
                     VGIDWTNKALILIVLAGVGSINGIFFAGLLLGIVEALGVYLVGVHYTGVVGLLIFIIV
                     LMLRPQGLFVRKAGV"
     gene            2351..3292
                     /locus_tag="PKJ17_00215"
     CDS             2351..3292
                     /locus_tag="PKJ17_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD74414.1"
                     /translation="MVERKKGLVIAAVVIVALALVPLFTGREYVFSILCMLFLYVTLS
                     QSWNILGGYTGQINVGQAAFFGMGALVARLLWVAGAPFYLSILAGGLSSVILAIIIGL
                     PALKLRGHYFAIGTLAVATIAQITVGNILPGISFLPAQQMATYSLIPRYYVFLIIMAA
                     ALAAIYITVNSRVGLGMEATRDDEEAAQMVGVNTFKYKMIAFVISSFFAGLAGGGFAY
                     FNASYYWYLPFTLMWCFEPILIVFIGGVGSFLGPVIGSVCYVLLKELFALTLGEANLL
                     IFGIVFILTILFFPKGIIGLPAKLRQLFYRRGNVVGR"
     gene            3362..4555
                     /locus_tag="PKJ17_00220"
     CDS             3362..4555
                     /locus_tag="PKJ17_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOD74415.1"
                     /translation="MAAVAFIFFSFVSVAAIAQEAPKSIKIGASISLTGRFAAGGIDV
                     ADGYKLAVKHINDKGGILVKQFNKKIPVELVVYDDESDPSKTVTRLEKLNAEKVVAYL
                     GEFASDMNIAGLGIAEKNKIPWVGVTIAVENVFNQGFKYIFGPNMFSFMEVAAFLDAL
                     DKIPESQRPKKIANFELQVDWGKECTKYLHEYAKKRGYTIALEKKFAPDTKDFSSMIM
                     AAKSEGCDAVFAVPTPPQSMALIKQMKELDYAPKATCFVRGPDLSNFWQVMGKDANYI
                     MTSGEWTPDMPYPGNKQLVADYRKLNPNKLMGMPVGNGYSAFQVLADAISRAEKLDRD
                     SIRQALTTTNIVTVKGKVTFDKRGVGNVPHVLAQWQNGELVVIVSPDYKTAPVLVATP
                     WSKRK"
     gene            4648..6297
                     /locus_tag="PKJ17_00225"
     CDS             4648..6297
                     /locus_tag="PKJ17_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD74416.1"
                     /translation="MPVAGFNEYRREDAQRYNDRRWWLGLTWGDLFDKATDTYPDREA
                     LVDDTRRFTYRELRDSVDRLAASFISLGIGQGDFVLLQLPNWNEFIISFFALQKAGAR
                     TVLLIARHGRSEINYLSELTQPKAWIVPETYGKINYEPLIADIRKDHACIEHVVLVRA
                     KQKTGHIDLEDLIRKGARDEASLRMIEERRPNPMELSLILPTGGSTGLPKAVPRTHND
                     FITNVEYHSRPWEITSEDTLLTVAPVSHGQGMLCGVGPAFLNYARYVLLDSTRPEDIC
                     AVIEREKVTALPTVPVLIQRLVNLEGLEKYDLRSLRKIYSGGAPSSPELVKAVYEKLE
                     CKFVNAYGSTEGPSAMTNLYDDMNTICTTVGRKDCPYTDFKVINQYGEELPPGTEGEL
                     VTKGPTIFTGYFKSPEDNAQIFTKDGYYKTGDLARIDDSGIITLTGRIKETILRGGET
                     ISAIGIERLIGSHPAIADVAVIGMPDKDLGERICAYVVRKAGTALTHEEIVAHLKGLG
                     ASVMQLPERTEFVASIPTTGVGKADKKILKQDIAKKLNYSL"
     gene            6308..8881
                     /locus_tag="PKJ17_00230"
     CDS             6308..8881
                     /locus_tag="PKJ17_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD74417.1"
                     /translation="MKYARLFSPLKVGSCVFPNRIMSTAAVTRLASEEGHITPNFVER
                     YRRLAEGGLGAMVVEAAVVLPSKSSFNLRISDDAYIEELKNFVDEIRKANREVKVGIQ
                     LIHFLKVARSGWRQKVEDLKPEEIAVIPGQFASSAKRARAAGFDFVELHMAHFTTLAS
                     FLSLVNKRQDEYGGDFEGRVKLPAEVVNVTREAVGDDYTIGVRMLGEEFTKEGNTLLQ
                     SARVARRLASLGVDYISVSAGERFEDADPPLENFPPFAGTGYSGYRMSPRWWNPDGVQ
                     VYLADGIRKAVRQAGYEVPIVCAGKVRMPDLAEKILEEGRSDVIGMARTLFADPDWPV
                     KAREGRADEIVKCAACGYCSEADERYETVVCIEWPKGALSAPSPWLLAPPCKAACPAG
                     LDIRSYIDLAAQGEYEKALAVIEEKIPFPASVSRVCPHPCERVCNRGTFDRPIAINGL
                     KRFVVDAVAKAGKREKVEPFPRTRDERVAVIGSGPAGMTAAFELAKLGYPVTVFEAAP
                     VPGGMLALGIPEYRLPKKMVREEIDAIEKRGVEIRLNTPVGEGGMPFGSLREEGFKAI
                     VVAIGAHRSVSPGIEGEGLEGVHHGVGFLKAVNLGVSVTIGEKVAVVGGGNVAVDAAR
                     TVHRMGAKEVTIVYRRSKDEMPAYPEEIEAAETEGVKIVFLSAPVEVTGKDGKATGLR
                     CVRTELGAPDESGRRTAHPVKDSEFEIAADCVILAVGETPDAGFFGSAGLPMKVQGDS
                     QWTGQEGIFACGDAVTGPATVIEAIASGRRTATGVHRYMCGESLDYTIAEPAVIPVED
                     VELERFKKREREKMPALNVPDRVGNFREVELGFTELMASSEAERCFQCGMFPRKDKNI
                     G"
     gene            8934..9335
                     /locus_tag="PKJ17_00235"
     CDS             8934..9335
                     /locus_tag="PKJ17_00235"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOD74418.1"
                     /translation="MSKECFNVFDEMYKRKKFTWSDAVKKGKVIAISGTVATDENHRT
                     LYAGDLNAQMRYIFESVKNLLEKMGASMDDVIKTTDYITPQAVPDYAKTAGIRREYFK
                     EDSYPAATGVVVHSLLRPDWLIEIDFLAVVD"
     gene            9407..10186
                     /locus_tag="PKJ17_00240"
     CDS             9407..10186
                     /locus_tag="PKJ17_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOD74419.1"
                     /translation="MDQGEVIVRKDGRVLYLTIKREKALNALNRNILEGLAVVFEGLE
                     KKRDASVVVMTGYGPKAFVAGADVREIKDAGAGRTEFIRMGQRVFHRIMTSPSVVIAA
                     VNGFALGGGCELALACDIRIASENASIGMPETGIGVMPGYGGTQLLSRLLHPGMAKYM
                     IFSGTPVNASEALQLGLVEKVYPEGVFMEEVEKLARKIASRGPIALECCKRAIDNGVG
                     LPLREALEVEIVEYDKVARSRDAEEGLSAFLEKREPVFTGT"
BASE COUNT         2335 a         2630 c         3223 g         2053 t
ORIGIN      
        1 ggataggtag aagagtatgg atctgttgaa ggtggagaat ctatcaaaat cgttcggggc
       61 cctctgggcg gtgaacaacg tgtccttctc ggtcgctgaa ggggagatag tgggccttat
      121 tggtcccaac ggttcgggca aatcgacggt gtttcatctc attaccggtc tgctcaaggc
      181 caacaagggc aggatcagct atcagggcaa ggacatcacg cgtttcaaac cctaccaggt
      241 atcacggtcc gggatagcgc ggacctttca gatcgtcaga ccctttcccg aaatgacgac
      301 cttgaagaac gtcatggtag gcagggcttt cggcagttcc agcgcgcgat cgatgcatga
      361 taccgaggac gatgcccggc ggctgcttga actgaccggc ctcgcggaca aggtgtccgc
      421 gacggcgggc cacctgggtc tcgtctacag gaagaagctg gaactcgcac gggcgctggc
      481 gacaaaaccc aggctccttc tgctggacga agtgatggcc gggttgaacc ctaccgagat
      541 gatggagacc gtggaatttc tgaaagggct ccggaatacg ggagtaacga tgatcgtggt
      601 ggagcacgtg ataaaggcgc ttttcagcat gacggacagg gttgtcgtgc tcaacgctgg
      661 agaaaagatc gccgagggta cgcctcacga gatctcccgg aacgaagaag tgatcaaagt
      721 ctaccttggg gattacgaac atgcttgaga ttacagggat agatgttttt catggtgacc
      781 tccaggctgt atgggacgtc tcgttgagta tagagggaaa ggggataacc accctcatag
      841 gctccaacgg gtccgggaag agcaccgtgc tgtcaaccat agcggggttg ctgaagccgg
      901 tcaggggaag catctccttc gacgggatgc gcatcgacac gctgcccgcg cataagatcg
      961 tcgatgccgg cattgccatg gtgcctgagg ggcgaaggct gttccgggaa atgaccgtga
     1021 tggacaacct cgaaatggga gccataacga aaagggccag gaagatgaag aacgagacca
     1081 tggcatggat cttcgatgtc ttccccatct tgaaggaaag ggccaggcag agggcggaca
     1141 cgctgagcgg cggtgaacag cagatgctgg cgataggcag gtcccttatg tcccagccga
     1201 ggcttctgct cgtggatgag ctgtctctcg gccttgcgcc ggtggtggtc cagaggataa
     1261 gccagactct ggacgagata aacaagacga aagggatagg catcttcctg gtggaacaga
     1321 atgtcgtcat ggccctggaa ctggctgaga ccggccacat tatcgagaac ggccgcattg
     1381 tgggaacagg aaaagcaagc gaactcctcg cgagcgatca ggtgagacag gcctatctcg
     1441 gaatggaagg cgaataaggc ttaaggagaa gatatggagt ttgctcaata catcacctat
     1501 ggggtcatca atgggtcgtt gtacgccctt gtcgccatcg gtcttgccct catcatggga
     1561 ataatgggga tcttcaacat agcccaggga tcgctggcta tgatagccgc gtatgggagc
     1621 gtcctctttt tccagcacat gaagatagac cccttcgtgt ccctggcgct tacgttgcct
     1681 gccatgtttc tcatcggcgt tttcatgtac ctgaccatgt tcatgtccat gaacaaattc
     1741 gatgccgggc agaagctgag gaattcgatg ctcgtttcct tcgggctggt cctgatcatg
     1801 gagaccctcg cgaccatact gtggacggca aacgagagga ctgtcaccac gggatactcc
     1861 ggcaccgtca tggccctcgg cgatctgcgg attccttaca ccggtctggc caccattgtt
     1921 atggccatag tcgtcatttt cgggctccac tccttcctga agcatacgac cttcggtaaa
     1981 tccatatggg ctgtgtccca ggaccatgac tgcgccatgc tcatggggat caacgtgaga
     2041 aggacgaccc tcgccgcctt tgggataggc gtcgcacttt ccgcgtttgc cggggcggtg
     2101 gtcagtcttc atacggtgag cccggctgtc gggatagatt ggacgaacaa ggcactcatc
     2161 ctcattgtgc tggccggtgt cggcagcata aacggcatct tctttgccgg tttgcttctc
     2221 ggtatcgtgg aagccctggg tgtctacctg gtcggtgtcc actacacggg tgtggtgggt
     2281 ctcctgatct tcattattgt gcttatgttg aggccgcagg gtcttttcgt gagaaaagcg
     2341 ggggtgtgac gtggttgaac ggaaaaaagg actggtaatt gctgctgttg tgatcgtcgc
     2401 cctcgctttg gtccctctct ttacgggcag ggagtatgtg ttcagcatac tatgcatgct
     2461 ctttctctac gtgacgcttt cgcagagctg gaacatcctg ggaggataca cgggccagat
     2521 aaatgtgggg caggcggcgt tcttcggaat gggcgccctc gtggccaggc tgctgtgggt
     2581 ggccggcgca cccttctatt tgtccatcct ggcgggcggc ctgagctcgg tcattttggc
     2641 aatcatcatc gggctgcctg cccttaagct caggggccat tattttgcca taggcaccct
     2701 cgcggtcgcg accatagccc agataaccgt cgggaacata ctcccgggga tcagcttcct
     2761 gcccgctcag cagatggcaa cgtacagcct gatcccccgc tactatgtct tcctgatcat
     2821 catggccgcg gcccttgcag ccatctatat cacggtcaat tcgcgggtcg gtctcgggat
     2881 ggaagcgacg agggatgatg aagaggcagc gcagatggtt ggtgtcaata ccttcaaata
     2941 caagatgatc gccttcgtga tcagcagttt cttcgccggt cttgccggcg gcgggtttgc
     3001 ctatttcaat gccagttact actggtatct tccttttacg ctgatgtggt gtttcgaacc
     3061 tatccttatc gtgttcatcg gcggagtggg cagtttcctc ggtcctgtta taggctctgt
     3121 ctgttacgta ctgcttaagg agcttttcgc cctgaccctg ggcgaggcga accttctcat
     3181 tttcggaatt gtgttcattc tcacaattct cttctttccc aagggcatta tcggcctgcc
     3241 ggcgaaatta cgccagttgt tctacaggag aggaaacgtt gtcggtcgtt aggtttgtgg
     3301 agtatcagta aaatctttaa ccaggaggtt tggaatgcag agtagacggt ttacattagt
     3361 tatggcagcg gtagcattca tcttcttctc gttcgtgagt gtggccgcca tcgcgcagga
     3421 ggcacccaag tcgatcaaga taggcgcgtc gatctccctg acgggacggt tcgcggccgg
     3481 agggatcgac gttgcggacg ggtacaaact ggccgtgaag cacatcaacg acaagggagg
     3541 gatcctggtc aagcagttca ataagaagat accggtggaa ctcgtcgtct atgacgatga
     3601 atcggatccc agcaagaccg tgacgcgcct ggagaagctg aacgcggaaa aggtggtcgc
     3661 ttaccttggc gagtttgcca gcgacatgaa cattgccggg ctgggtattg ccgagaagaa
     3721 caagatcccc tgggttggtg tgacgatcgc ggtggagaac gtgttcaacc agggcttcaa
     3781 gtatattttc ggtccgaaca tgttctcgtt catggaagtg gccgctttcc tcgatgccct
     3841 tgacaagatc cctgagagcc agaggccgaa aaagatagcg aacttcgaac ttcaggtaga
     3901 ctggggcaag gaatgcacca aatacctcca cgagtatgcc aagaaacggg gttacaccat
     3961 cgcgctcgaa aagaagttcg cccccgacac gaaggacttc tcctccatga tcatggccgc
     4021 caagtccgag ggttgcgacg ctgttttcgc ggtcccgacg cctccccaga gcatggccct
     4081 gatcaagcag atgaaagaac tcgattacgc cccgaaagct acctgttttg tcaggggtcc
     4141 ggacctctca aatttctggc aggtgatggg gaaggacgcg aactatatca tgacctctgg
     4201 tgagtggacg cccgatatgc cctatcccgg aaacaagcag ctcgtcgccg attatcggaa
     4261 actcaatccc aacaagctga tgggtatgcc tgtcggaaac ggatattccg cgttccaggt
     4321 cctggcagac gcgatctccc gcgccgagaa gctggaccgt gattccatac ggcaggccct
     4381 gaccacgacc aacatagtga cggtaaaagg gaaggtcacc tttgacaagc gcggagtcgg
     4441 caacgttccg catgttctgg cacagtggca gaatggcgag cttgttgtca tcgtcagccc
     4501 cgactacaag acggcaccgg tcctcgtggc aacgccatgg agcaaacgga agtgaacagg
     4561 taacaagaag cgggaaggcc ctccgggcaa ccggagggcc ccacatcaga acagcagcga
     4621 tgtgtttcgc agcaaggagg gagaactatg cctgtcgcag ggttcaatga atacagacgc
     4681 gaagacgcgc aaagatacaa cgatcgcaga tggtggctcg gcctgacctg gggagatctc
     4741 tttgacaagg ccacggacac ctacccggac agagaagccc tggtggacga tacgaggagg
     4801 tttacgtacc gggagttgag agatagcgtg gatcggctgg ccgcgagctt tatctctctt
     4861 gggatagggc agggagattt cgtgctgctt cagcttccga actggaacga gttcatcatt
     4921 tcctttttcg ccctgcaaaa ggcaggcgcg cgaacggttc tcctcatcgc ccgtcacggg
     4981 agatcggaga taaactactt gtccgagctt acccagccga aggcgtggat cgtccccgag
     5041 acctacggca agatcaacta tgagcccctg atagccgaca tacggaagga ccatgcctgc
     5101 attgaacatg tcgtcctggt cagggcgaag cagaagacag gacatatcga tctcgaagac
     5161 ctgatccgga agggcgcgcg cgacgaggca tcgctcagga tgatagagga acgaagaccc
     5221 aacccgatgg agttgtccct tatcctgccg acaggcggtt cgacaggcct tcccaaagcg
     5281 gtgccgagaa cgcacaacga tttcatcacc aacgtcgagt accactcgag gccctgggag
     5341 atcacaagcg aagacacgct cctcactgtc gctccggtca gtcacggcca ggggatgctc
     5401 tgtggcgtcg ggcccgcttt cctcaactac gcgaggtacg tgcttctcga ttccaccaga
     5461 ccggaggaca tatgcgcggt gatcgaacgc gaaaaggtca cggcgcttcc gacggttccg
     5521 gtcctgatcc agagacttgt caacctggag gggcttgaga aatacgacct gcgttcgttg
     5581 agaaagatct attccggtgg cgcaccgagc agtccggagc tcgtaaaggc cgtatacgag
     5641 aaactggagt gcaagttcgt gaacgcctat ggttcgactg aaggtccgtc cgccatgacg
     5701 aatctttatg acgatatgaa caccatctgc accaccgtgg gcagaaagga ctgcccgtac
     5761 acggatttca aggtgatcaa ccagtatggt gaggaattgc ctcccggaac ggagggtgaa
     5821 ctcgtgacga aagggccgac gatcttcacg gggtatttca aatcgcccga agacaacgct
     5881 cagatcttca cgaaggacgg ctattacaag accggcgacc tggcgaggat cgatgactcg
     5941 gggatcatta cccttacggg gaggataaaa gagacgatcc tcaggggcgg agagactatc
     6001 agcgcgatcg ggatcgaacg gcttatcggc tctcatcccg ccatagcgga tgtggcggtc
     6061 ataggaatgc ccgacaagga ccttggcgag cgcatctgcg cctacgttgt ccgcaaggcc
     6121 ggcaccgctc tcacccacga ggagatagtg gcgcatctga agggattggg cgcatcggtc
     6181 atgcaactcc cggaaaggac cgagtttgtg gcatcgatcc ccacgaccgg tgtgggcaag
     6241 gcggacaaga agatcctgaa acaggacatt gcaaaaaaac taaattattc actctgagag
     6301 gttcgagatg aagtatgcaa gattgttctc cccattgaaa gtcggaagct gcgtattccc
     6361 caaccggatc atgtcgacgg cggcggtcac gagactggcg tcggaggaag ggcacataac
     6421 gccgaatttc gtcgaaaggt acaggagact ggctgagggc gggctgggcg caatggttgt
     6481 cgaggccgcc gtagtgctcc cgtcaaaaag ctcctttaat ctgcggatca gcgatgacgc
     6541 gtatatagag gaattgaaga acttcgtcga cgagatcagg aaggccaata gagaggtcaa
     6601 ggtggggatc cagctcattc atttcctcaa ggttgccaga agcggctgga ggcaaaaggt
     6661 tgaggatctg aaaccggaag agatagccgt aatacccggt caatttgcca gctccgccaa
     6721 acgggcgagg gcggcgggtt tcgatttcgt ggagcttcac atggcccatt tcacgaccct
     6781 tgcttcgttc ctgtccctgg tgaacaagag gcaggatgaa tacggaggtg acttcgaggg
     6841 gcgcgtgaaa ctgcccgcgg aggtagtgaa tgtcaccagg gaagccgtgg gtgatgacta
     6901 taccatcggc gtgagaatgc tgggtgagga attcacgaag gaaggtaata cgcttctgca
     6961 aagcgcgcgc gtggcgagac ggctcgcttc cctgggagtc gactatatca gcgtctccgc
     7021 tggagagaga tttgaggatg ccgatccccc tctcgagaat tttcccccat tcgcgggcac
     7081 cggttacagc ggttacagga tgagcccgag atggtggaac ccggacggcg tgcaggtcta
     7141 tctggcggac ggcatacgga aagcggtgag acaggccggc tacgaggtgc ccatcgtgtg
     7201 cgccggcaag gtcaggatgc ccgatcttgc cgagaagata cttgaagaag gaaggtcgga
     7261 tgtcataggc atggcgagga ccctttttgc cgaccctgac tggcccgtga aggcgagaga
     7321 aggcagggct gacgagatcg tgaaatgcgc cgcctgcggg tactgcagcg aggcggacga
     7381 aaggtatgaa accgtcgtct gcatcgaatg gcccaagggc gccctgtccg ctccatctcc
     7441 gtggctcctt gcgcctccat gcaaggccgc ctgcccggca ggattggata tccggagcta
     7501 catcgatctg gctgcacagg gagagtacga aaaggccctg gcggtgatcg aagagaagat
     7561 cccctttccc gcatcggtga gccgggtgtg cccgcatccg tgtgagagag tatgcaacag
     7621 gggcacgttc gaccgaccga tcgcgatcaa cgggttgaaa cggtttgtcg tggatgccgt
     7681 ggcgaaagcg ggaaaaaggg agaaggtcga gccttttccg aggacgcggg atgaaagggt
     7741 tgccgtgatc ggttcgggac cggcgggcat gaccgccgcc ttcgagctcg cgaagctcgg
     7801 ttaccccgtt acggtctttg aagcggcccc cgtacccggc ggcatgctgg cgctcggcat
     7861 tcccgagtat cgcctgccga agaagatggt aagggaagag atcgatgcca tcgaaaaacg
     7921 cggcgtggag atacggctca atacccctgt cggtgaaggc ggcatgcctt tcggcagcct
     7981 gcgcgaggaa gggttcaagg cgatcgttgt cgccatagga gcccacagga gcgtgagccc
     8041 cgggatcgag ggcgaagggc tcgagggtgt gcatcatggt gtgggtttcc tgaaggcggt
     8101 gaacctggga gtctctgtga ccatcgggga aaaggtcgcg gtcgtcggcg gtggcaacgt
     8161 tgccgtcgat gcggcaagga cggtccaccg catgggagcg aaagaggtca ccatagtcta
     8221 ccggagatcg aaagatgaga tgcccgcata cccggaggag atcgaggcgg ctgagaccga
     8281 gggcgtgaag atcgtcttcc tgtcggcacc ggttgaggtg acagggaagg acgggaaggc
     8341 gacgggtttg agatgcgtga ggacggagct cggcgcgccg gatgaaagcg gccgccggac
     8401 ggcgcatccc gtaaaggact cggagtttga gatcgccgcg gattgtgtga tcctcgcggt
     8461 gggcgaaacg cctgatgccg gcttcttcgg gtctgcgggg cttcccatga aggtgcaagg
     8521 ggattcgcaa tggaccggcc aggaaggcat atttgcctgc ggcgatgcgg taacagggcc
     8581 ggcgaccgtc atcgaagcca tcgcgtcggg ccgccggacg gcgacgggtg ttcacaggta
     8641 catgtgcggt gaatccctcg actatacgat agcggagccc gcggtgatcc cggtggagga
     8701 cgtcgaactg gagcgtttca agaagaggga acgggagaag atgcccgccc tcaacgtgcc
     8761 cgacagggtt ggcaatttca gggaagtgga gcttggcttc acggagctga tggcgtcaag
     8821 cgaggctgag cggtgtttcc agtgcggtat gttcccgagg aaggacaaga acatcgggta
     8881 gtccgcaccg gggcaacacg agcaccagca acaacataga tggaggtacc acaatgtcaa
     8941 aagaatgttt taacgtgttc gatgaaatgt acaagcggaa gaaattcacc tggagtgatg
     9001 cggtaaagaa aggcaaggtc atagccatct cgggtacggt ggccaccgac gagaaccacc
     9061 ggacgctcta cgcgggcgat cttaacgcgc agatgcgcta tatattcgaa agcgtcaaga
     9121 acctgctcga aaagatgggc gcgagcatgg acgacgtcat caaaacgacg gattacatca
     9181 cgccccaggc cgtccccgac tacgcgaaaa cggccggcat acgaagggag tatttcaaag
     9241 aggacagcta tccggccgcc acgggagttg tggtgcacag cctcctcagg cctgactggc
     9301 ttattgagat cgattttctt gcagtcgtgg actgatagcg acaagagcga cgtgagcagg
     9361 gcaggccctg cggaatctca gcggggcctg cccacgagag gagaatatgg accagggtga
     9421 ggtgatcgtc agaaaggatg ggagagtgct ctatctcacg ataaagcgtg agaaggcgct
     9481 caacgctctc aacaggaaca tactggaggg acttgcggtc gtcttcgaag gtctcgaaaa
     9541 aaaacgtgat gcaagcgtgg tggtcatgac cggatacggt ccgaaggcct tcgtggccgg
     9601 cgcggacgtg cgggagatca aggatgcagg cgcgggcaga acggagttca tcaggatggg
     9661 acagagggtt ttccaccgga taatgacgtc ccccagcgtg gtcattgccg ccgtgaacgg
     9721 gttcgcactg ggaggaggtt gcgagctggc gctggcctgc gacatacgca tcgcgtcaga
     9781 gaatgcctcc atcggcatgc ccgagacagg cattggagtg atgcccggct atgggggaac
     9841 gcagttgctc tcccgcttgc tgcaccctgg aatggcgaaa tacatgatct tttcgggaac
     9901 ccccgtgaat gcctctgagg ccctgcaact gggcctcgtc gaaaaggtct accctgaggg
     9961 cgtgttcatg gaagaagtgg aaaagctcgc gcggaagatc gcttcgagag gtccgatcgc
    10021 cctcgagtgc tgcaagaggg ccatcgacaa cggagtgggg ctgcccttgc gggaagccct
    10081 ggaggtggag atcgtcgagt acgacaaggt ggcccgttcc cgggatgccg aagagggtct
    10141 ctccgccttt ctcgagaaga gggaaccagt cttcaccgga acgtagataa ggagatcaca
    10201 atggaaataa agaaactggg cgtattggga tgtgggcaga t
//
LOCUS       DAOPOA010000006         9856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_7814_length_9856_cov_3.492909, whole genome shotgun sequence.
ACCESSION   DAOPOA010000006 DAOPOA010000000
VERSION     DAOPOA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7814_length_9856_cov_3.492909"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /locus_tag="PKJ17_00245"
     CDS             complement(<1..1977)
                     /locus_tag="PKJ17_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD74420.1"
                     /translation="MHGLLRRQLKKAFGEAFSVPPEWKGFIEMVDTAYRESDTDRDML
                     ERSLELSSQELSEMNSEMRAIFEAIPDLLFRVDREGIILDCKTGNTSDLVFARERLIN
                     RKIDDIPVPSVSRQFAEAMDAVRRTKSPANAEYSLNIDGNEQFYEARFIPSAKGQIVI
                     MVRNVTERRRAEKALMESEEKYRSIFENSIEGIFQTTRDGEYVEANPALVHMLGYGSQ
                     EEMLRESGTVTRQYVVPDDRKRFRELIERHDVLQGFETRVYRRDGSRIWISINAHVVR
                     DRKGNIVFYEGTVENITDRKRAEEALFESEEKYRSIVEGSHVGVYIIEDGLFRFVNAT
                     LCDILGYTYGEIADTMGPGDIILPEDFPAVGESLQKRLSGEIKTAELIYRIRRKDGEI
                     RSIKAIGSSILYRGRPAVIGTLLDITKEKELEAQLLQSQKLETVGRLAGGIAHDFNNM
                     LGVILGNVQMAKINLSASEHTYGFCDAIERATIKAADLVRQLLAFSRRQLLELKTFDL
                     NDLVSGFEKMIHRVIAENIDMTFILAKDLPAIKADETQMNQVLLNLVVNARDAMADGG
                     ILTISTGNVDVEAKYVQTHPDVRPGRYVMLSVADTGIGMEKDVFDRMFEPFFTTKAGG
                     SGLGLSVVYGIVKQHGGFVEVMSERGRGTTIQVFL"
     gene            complement(1986..3128)
                     /locus_tag="PKJ17_00250"
     CDS             complement(1986..3128)
                     /locus_tag="PKJ17_00250"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOD74421.1"
                     /translation="MKISQRFLAGQTASPLRGAVAPDETADLVLAFGSKELLNDGDSF
                     GALRALYPAAHLCGCSTAGEICGTGVFDDTLAATVISFEGTRIMGAGITLTAVSNSKE
                     AGALLARSIEMEGLVHVFVLSDGLNVNGSELVEGLSGSLPEKVTITGGLAADADRFEE
                     TYVFWDGPPRQNSLAIVGFYGNHLKVGYGSMGGWDPFGPDRLVTRSSGNVLYEIDGRS
                     VLDLYRRYLGDRARDLPSSGLFFPLCVRTGEGEKGIVRTLLSVNEKEGSMTFAGDIPQ
                     GSYARLMKANFERLIDGASGAAATAREAMGSVPVELAVLISCVGRKLVLHQRVEEEIE
                     ASREVLGPDAVVTGFYSYGEISPFTPGGKCALHNQTMTVTTLSEVR"
     gene            3312..3863
                     /locus_tag="PKJ17_00255"
     CDS             3312..3863
                     /locus_tag="PKJ17_00255"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74422.1"
                     /translation="MRRIVVDIDNTLWDLAPVLCERLQEASPGFPHHSQWHDWSFWKG
                     LIPADRLYGIIRDVHMEQDTFEPYRDSRDFLQRLRGKGFHIVIASHRERETYASTERW
                     LEKHGLPFDEIHLSYDKTVLFDDCWAIVDDSPVTLEKARQAGIVRAGLRNPWNESEEH
                     PLFADLGEIYRYLETQCTAGKRD"
     gene            4005..4421
                     /locus_tag="PKJ17_00260"
     CDS             4005..4421
                     /locus_tag="PKJ17_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOD74423.1"
                     /translation="MKSYRKELWFNVPARRGFVNITPQVEECLRESGVSEGLVLVNAM
                     HITASVFVNDDESGLHHDFDVWLERLAPHEPVSGYRHNVGEDNADGHLKRQIMGREVV
                     VAITDGRLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            complement(4580..7792)
                     /gene="carB"
                     /locus_tag="PKJ17_00265"
     CDS             complement(4580..7792)
                     /gene="carB"
                     /locus_tag="PKJ17_00265"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOD74424.1"
                     /translation="MKKPPKIKGIDKVLIIGSGPIIIGQACEFDYSGTQACKALRAAG
                     YKIVLVNSNPATIMTDPGMADHTYVEPLTIDVIEKIIEKEKPQGLLPNLGGQTGLNLA
                     AELSRRGILEKHGVRIIGVQADAIERGEDRDEFKKTMKRLGIPMPESALAYSVEEALE
                     IADRLGYPVVIRPAYTMGGTGGGIAFNKEELRTIASRGISASIIGQILIEEAVVGWEE
                     LELEVVRDADNRMITVCFIENIDPMGVHTGDSFCCAPMLTISEEVQKKLQDYSYRIVE
                     AIQVIGGTNIQFAHNPEDGRIVVIEINPRTSRSSALASKATGFPIAFISAKLAGGYTL
                     KDIPYYRGKGLDEYVPTGEYVVIKFARWAFEKFSEAEDRLGTQMKAVGEAMSIGKTYK
                     EAFQKAIRSLETKRYGLGFAKNFNQLPLDDLMRLLNEPSSERQFIMYEALRKGAGIDE
                     LHEKTKIKRWFIEQMKEILDLEEEILRHKGGTLPDALLAKAKEDGFSDRYLSFLLGVP
                     ETDIRKKRQTLGKNETWCRVPVSGADAAYYYSTYNAPDEVAVSDRRKVMILGGGPNRI
                     GQGIEFDYTCVHAAFTLRDEGYESIMVNCNPETVSTDYDTSDKLYFEPLTVEDVLSIY
                     EKEKPVGAIVQFGGQTPLNLARQLEEAGVNVLGTSPESIDIAEDRKRFSQIIKKIGIP
                     QPESGTAVVLEEALEAAHRIGYPLMVRPSYVLGGRGMEVVYDDEMLKAYVNAAVDISP
                     GRPILIDKFLENAIEVEADAISDGVDAFVPSIMEHIELAGVHSGDSACAIPPRTLSEE
                     HIATINEYTKKLAIELKVVGLMNIQYAIAEDKVYILEANPRASRTVPLVSKVTGVQMA
                     RIATQLMLGRKLKDMKLTQKTYPHVGVKEAVFPFNMFPEVDPTLGPEMKSTGEVLGMA
                     NSFGLAFAKSQEAAGQKLPRSGNVLITVVDRDKDGIVDVAKQLAKLGFTIVATEGTHR
                     FLSDNGVKSTLIKKVHEGRPNIVDAIHNKEIHLVINTPSGKSSMYDDSYIRKTAIKYK
                     IPYITTTAAAKATAEGIKASVAKKGEIKVKSLQSYHRAIK"
     gene            complement(8052..8984)
                     /locus_tag="PKJ17_00270"
     CDS             complement(8052..8984)
                     /locus_tag="PKJ17_00270"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOD74425.1"
                     /translation="MMDPLLAEVWGVRLWYYGLAWALGFSAVLLWVMLRRNYVGLTSR
                     QVWDLSLVFSLSCLVLGRVFQVLVYEWDIFNGRYAEVFAFWKEGMAYEGVMVGALAGA
                     VLFCAVHGKSFFLVADEMVIPFAFLLALVRIGGHLNGEIYGYVTTAWWAVKFPLVEGF
                     RHPVALYDAFKNLIIMFILLAVGRGGTQGQGRLLGHFMLWYGVFDLVIDIFQAPGPLF
                     RDFGIGRFSGALVALIGLLLIARSARRNSRKTTELSTLRFAPASLIAAIPSRLTPGIW
                     FRVMLFMAILLFCLTIPGGWSREWLHHLAWQLGI"
     gene            complement(9482..>9856)
                     /locus_tag="PKJ17_00275"
     CDS             complement(9482..>9856)
                     /locus_tag="PKJ17_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOD74426.1"
                     /translation="AIMKYLDGNTYHPSAGDIYGALRDQFPTMSLATVYNTMEALKEQ
                     GVVIELSTDRGKKRFDPNPKPHHHLMCIKCKAIVDVFIEFPLTLPDAQTQGFEILGNR
                     VDFYGICKSCSDEQKRMMETNQ"
BASE COUNT         1939 a         2773 c         2948 g         2196 t
ORIGIN      
        1 aagaaagacc tgtatcgtcg taccccgtcc gcgttcgctc atgacctcta cgaaacctcc
       61 gtgctgcttg acgatcccgt agacgacgga aagtccgaga ccgcttccac cggccttggt
      121 ggtgaagaag ggttcgaaca tcctgtcgaa aacatctttt tccatcccaa tcccggtgtc
      181 ggcgacggag agcatcacat agcggcccgg tcgcacgtcg ggatgggtct ggacatattt
      241 cgcctcgaca tccacattac ccgtggagat ggtgagtatc cctccgtcgg ccatggcgtc
      301 cctggcgttt acgacgagat tgagcaggac ctggttcatc tgtgtctcat cggccttgat
      361 cgccgggaga tccttcgcca ggataaaggt catatcgatg ttctcggcga tgacacggtg
      421 tatcatcttt tcaaagcccg agacgaggtc gttgagatcg aaggtcttga gttccagaag
      481 ctggcgtctt gagaacgcga ggagctggcg caccaggtcg gccgctttta tagtcgccct
      541 ttcgatggcg tcacagaaac cataggtgtg ttcgctggcg gacagattga tcttcgccat
      601 ttggacattt cccaggatca ccccgagcat gttgttgaaa tcgtgggcga taccgccggc
      661 gagccttccc accgtttcca gcttctggga ttgcagaagc tgcgcctcga gttctttttc
      721 cttggtaatg tccaggaggg tcccgatcac ggcggggcgt cccctgtaaa ggatggagct
      781 tccgatcgcc tttatggacc ggatctcccc gtcctttctg cggatgcgat agatcaactc
      841 cgctgtcttg atctcgcccg agaggcgttt ttggagactt tcgccgacgg cggggaagtc
      901 ctctggcagt atgatgtccc cggggcccat cgtgtccgct atctcgccgt aggtgtaccc
      961 gagtatatcg cagagtgtcg cgttcacgaa gcgaaaaagg ccgtcctcga tgatgtagac
     1021 gccgacatgg gacccttcga cgatgctcct gtatttctct tccgattcaa aaagggcctc
     1081 ttcggcccgc ttcctgtcgg taatgttctc taccgtccct tcataaaaga caatgttccc
     1141 ctttcggtcc ctgacgacat gggcattaat ggatatccat atcctgctgc cgtccctgcg
     1201 atagacccgg gtctcgaacc cttgaagcac atcgtgcctt tcgatgagtt ccctgaagcg
     1261 cttccgatcg tcgggcacga catactgacg ggtcacggtg ccggactcac ggagcatctc
     1321 ttcctgtgat ccatacccca gcatgtggac aagggcggga tttgcctcga catactcgcc
     1381 gtcacgcgtg gtctggaata tgccctcgat ggagttctcg aatatgctgc gatatttctc
     1441 ctcgctttcc atgagggcct tttccgcccg tcgccgctcc gtcacgttcc gcaccatgat
     1501 aacgatctgg cccttcgcgg aaggtatgaa gcgcgcctcg tagaactgct cgttcccatc
     1561 gatattcaac gagtactcgg cgttggccgg agacttcgtt ctcctcacgg cgtccatggc
     1621 ctccgcgaat tgcctgctta cggatgggac gggtatgtcg tcgatcttcc ggttgatgag
     1681 ccgttcgcgg gcgaagacga ggtccgaggt gtttcccgtc ttgcagtcca ggatgatgcc
     1741 ttcgcggtcc acccggaaga gcaggtccgg tatcgcttca aagatggccc tcatttccga
     1801 gttcatctcc gagagctcct gggaactcag ttccagggag cgttccagca tgtccctgtc
     1861 cgtgtccgat tcgcggtagg cggtgtcgac catttcgatg aaccccttcc attcgggcgg
     1921 caccgagaag gcctcgccaa aggccttctt cagttgccga cgcaggaggc cgtgcattct
     1981 tgtcactacc gtacctccga cagggtcgtc accgtcatgg tctggttgtg gagagcgcat
     2041 ttcccgccag gggtaaaggg ggagatctca ccgtaggagt aaaagccggt gacgacggcg
     2101 tcagggccga ggacctcccg ggacgcctcg atctcctctt cgacgcgctg gtgtaaaacc
     2161 agctttctgc ccacgcagct gatgagtacg gcgagttcga cagggacgga gcccatggcc
     2221 tcgcgggcgg tcgccgcggc cccggaggca ccgtctatga gcctttcaaa gtttgccttc
     2281 atcagccggg cgtatgaacc ttgcggaatg tcgccggcga aggtcatcga accttccttc
     2341 tcgttcacgg acaaaagggt gcggacgatg cctttctccc cttcacccgt ccggacgcag
     2401 agagggaaga ataggcccga cgaaggcaga tcgcgcgccc tgtcgccaag atatctcctg
     2461 tagaggtcca gcaccgacct gccgtctatc tcgtaaagca cgttgcccga ggatctcgta
     2521 acgagacggt caggaccgaa ggggtcccag ccgcccattg agccgtagcc gaccttcaga
     2581 tggtttccgt agaagccgac gatggcaagg gaattctgtc ggggtggccc gtcccagaac
     2641 acataggttt cctcgaaacg atccgcgtcc gccgcaagac cacctgtgat ggtaactttt
     2701 tcgggcaggc ttccggagag tccttcgacg agttcgctgc cgttgacgtt caggccgtcg
     2761 gagagaacga atacgtggac aaggccctcc atctcgatgg aacgggccag cagggcgccg
     2821 gcttccttgc tgttggagac ggccgtgagg gtgatgcccg cccccatgat gcgcgtccct
     2881 tcgaatgata taaccgtggc ggcgagcgta tcgtcgaaga cccccgtacc gcagatctcg
     2941 ccggcagtgg agcaaccaca gagatgggcg gcgggataca gggcccgaag ggccccgaag
     3001 gagtcgccgt cattgagaag ctccttcgag ccaaaggcca gaacaaggtc cgcggtctca
     3061 tcgggagcga cggctccgcg cagcggcgaa gctgtttgtc cggcaaggaa cctctgcgag
     3121 atcttcataa tcttccccct tcaggtattc ccgatgacat gatccctgtt cccgggtgtg
     3181 acccggctcg tgcccccgga cataaagttt tctcgtcgga acggcgagag cccttaagca
     3241 ttcttcccca agcgatgatg ataccccacc ctgttttgga aatcacgctt tttgtggtac
     3301 aatgagtcgt tgtgcgaagg atagtcgtcg atatcgataa taccctgtgg gacctggcgc
     3361 cagtgctctg cgagcggctg caggaggcca gtcccgggtt cccccaccac tcacagtggc
     3421 acgactggag tttctggaag gggctcatcc cggcggaccg gctctacggc atcatccggg
     3481 acgtccacat ggagcaggat acctttgaac cctaccgcga ttcccgcgac tttctccaaa
     3541 ggcttcgcgg aaaaggcttt cacatagtca tcgccagcca tcgggagagg gagacctatg
     3601 cctccacgga gaggtggctt gagaagcacg gactgccctt cgacgagatc cacctgagct
     3661 atgacaagac ggtgctcttc gatgactgct gggcgatcgt cgacgacagc cccgtgaccc
     3721 tggaaaaagc caggcaggcc ggaatagtca gggccggcct caggaacccc tggaacgaat
     3781 ccgaagagca ccctctcttc gcggacctgg gggagatcta ccggtatctt gagacccagt
     3841 gcaccgcggg aaagagagac tgacccgagg gttcttaagg tgtcctatat cctacaagta
     3901 tcctatcgct tctgaatagg acctatagga ccaatacgac gtataggacg cttaggacct
     3961 acaggacaga cacggggtcc ataggacgca gagagggcga aggcttgaaa agctacagaa
     4021 aagagctctg gttcaatgtg cccgcccggc gggggtttgt gaatattacg ccgcaggtgg
     4081 aggaatgcct gcgtgagagc ggggtctccg agggccttgt cctcgtgaac gccatgcata
     4141 tcacggcgtc ggtcttcgtg aacgatgacg agtcgggcct ccatcacgac ttcgatgtct
     4201 ggctcgagag gctcgccccc cacgagcccg tctcgggcta caggcacaat gtcggtgagg
     4261 acaacgccga cgggcactta aagcgccaga tcatgggcag ggaggtggtc gtggccataa
     4321 cggacggccg cctcgacttc ggcccctggg agcagatatt ctacggcgaa ttcgacggcc
     4381 gcagaagaaa acgggtgctc gtcaagatca taggagagtg aaagacggct tgagccgatt
     4441 gaatggcttg aatcgcttga acgccgggaa agaaacacga accctataag tcccatgcgt
     4501 cctgtaggtc ttataggtcc tattccagaa gaagcggcag cccctgcgca tcccaaagga
     4561 tgggcagggg ctgttttttt tattttatcg ccctgtggta ggattgcagt gattttacct
     4621 tgatctcgcc tttcttggcc acggatgcct tgatgccctc ggcggtggcc ttcgcggcgg
     4681 ctgttgtggt gatgtagggt atcttgtact tgatggccgt ctttcggatg taggaatcgt
     4741 catacatgct gctcttgcct gagggcgtgt tgatgacgag gtggatctcc ttgttgtgga
     4801 tggcgtcgac gatgttgggc cggccctcgt gcactttctt gatgagggtg cttttcacgc
     4861 cgttgtcgga gaggaaccgg tgggtcccct cggtggcgac gatggtgaat cccagtttgg
     4921 caagctgttt cgcgacatcg acgatcccgt ccttgtcgcg gtcgacgacg gtgatgagaa
     4981 cgttgcccga gcgggggagc ttctgaccgg cggcctcctg ggatttcgcg aaagccaggc
     5041 cgaaggagtt ggccatgccc aggacctcgc cggtggactt catctccggg ccaagggtcg
     5101 ggtcgacctc ggggaacatg ttgaagggga agacggcctc cttgacgccc acgtggggat
     5161 atgtcttctg cgtgagtttc atgtccttga gcttcctgcc cagcatgagc tgtgttgcga
     5221 tgcgggccat ctggacaccc gtcaccttgg agacgagcgg taccgttctc gacgcccggg
     5281 gattcgcctc gaggatgtag accttgtctt cggcgatggc gtactggatg ttcatgagac
     5341 ccacgacctt gagttcgatg gcgagcttct tcgtgtattc gtttattgtg gcgatatgct
     5401 cttccgacag tgtccggggg ggtatggcgc aggcgctgtc tcccgagtgg actccggcga
     5461 gctctatgtg ttccatgatg gagggtacga aggcatcgac gccgtcggag atggcgtccg
     5521 cctctacctc tatggcgttc tcgaggaact tgtcgatgag gatgggcctg ccgggagaga
     5581 tgtccacggc ggcattcacg taggccttga gcatctcgtc atcgtagacg acctccatgc
     5641 cgcgtccgcc aaggacatat gaggggcgga ccatgagggg gtagccgatc ctgtgcgcgg
     5701 cttccagggc ctcttcgaga acgacggcgg tgccgctctc gggctggggg atgcctatct
     5761 tctttatgat ctggctgaag cgtttgcggt cttccgcgat atcgatgctt tccggggatg
     5821 tcccgaggac gttcacgccg gcctcttcga gctgccgtgc caggttgaga ggggtctgcc
     5881 cgccgaactg gacgatggcg ccgacgggtt tttccttctc gtaaatgctc aggacatctt
     5941 ccaccgtgag gggctcgaag tagagcttgt ccgaggtgtc gtagtcggtg gagacggtct
     6001 ccggattgca gttgaccatg atggattcgt atccctcgtc gcgcagggtg aatgcggcat
     6061 ggacgcacgt gtagtcgaac tctatcccct ggccgatccg gttggggccg ccgccgagga
     6121 tcatcacctt cctgcggtcg ctgaccgcca cttcgtcggg ggcgttatag gtcgagtaat
     6181 aataggccgc gtcggcgccg ctgacgggta cgcggcacca tgtctcattc ttgccgaggg
     6241 tctgccgttt cttcctgatg tccgtttcag ggacgccgag gaggaaggag agatacctgt
     6301 cggagaatcc gtcctctttt gccttggcga ggagggcatc ggggagcgtg cctcccttgt
     6361 ggcgaaggat ctcttcctcg agatcgagta tctccttcat ctgttcgatg aaccaccgtt
     6421 tgatcttcgt cttctcatgg agttcatcga tgccggcacc cttgcgcagc gcctcgtaca
     6481 tgatgaactg cctttcagag gagggttcgt tgagcaggcg catcaggtcg tcgagaggca
     6541 gttgattgaa attcttggcg aaaccaagcc cgtagcgttt tgtctccagg gaacggatcg
     6601 ccttctggaa cgcttccttg taggtcttgc cgatgctcat agcctcgccg acggccttca
     6661 tctgggtgcc cagcctgtcc tctgcctcgc tgaacttctc gaaggcccag cgggcgaact
     6721 tgatgaccac gtactctccc gtagggacgt attcgtcgag ccccttcccg cggtaatagg
     6781 ggatgtcctt cagggtgtag ccgcctgcca gcttggccga gatgaaggcg atggggaagc
     6841 ccgttgcctt cgaggcgaga gctgacgagc gggatgtcct ggggttgatc tcgatgacga
     6901 caatgcggcc gtcttcgggg ttgtgggcga attgtatatt ggtgccgccg atgacctgga
     6961 tggcctccac gatccggtag gagtaatcct gaagcttctt ctgcacctcc tcggaaatgg
     7021 tgagcatcgg cgcgcagcag aatgagtcgc cggtgtggac gcccatggga tcgatgttct
     7081 cgatgaagca gacggtgatc attctgttgt ccgcgtcacg gacgacctcg agttccagct
     7141 cttcccagcc caccacggct tcctcgatga ggatctgccc gatgatgctg gcggagatgc
     7201 cccggctcgc tatggtccgc agctcctcct tgttgaaggc tatgccgcct cccgtgccgc
     7261 ccatggtgta cgcggggcgg atgacgacgg gatatcccaa cctgtccgcg atctcgaggg
     7321 cttcttccac ggagtaggcg agagcgctct cgggcatggg aatgcccagt ctcttcatgg
     7381 tcttcttgaa ctcgtcacgg tcctcgccgc gctctatcgc gtcggcctgg actcctatga
     7441 tgcggacccc gtgcttctca agtatccccc ggcgggacag ctctgcggca aggttgagac
     7501 ccgtctgacc tcccagattg gggaggagcc cctggggttt ctccttttct atgatctttt
     7561 cgatgacgtc gatggtgagg ggttccacgt atgtgtgatc cgccatcccg ggatcggtca
     7621 tgatcgtcgc ggggttggaa ttcacgagga ctatcttgta tccggcagcc ctcagggcct
     7681 tgcaggcctg ggtgccggag tagtcgaact cgcacgcttg cccgatgatg atgggaccgg
     7741 agccgatgat gaggaccttg tcgatgcctt tgattttggg gggttttttc attgccgggg
     7801 attcctttcg gatttgctct gcatcgtaac agatgagtgg cagtcagtca agcgttttca
     7861 aaggatttcg ggttttggta accggtggcg ggataaaaat agtgacctgt tcgccaacta
     7921 cttgtagtta caggagaaac tatgaccaac cgggcggtga tcgacggccc ggaagggata
     7981 ctctggagag gggagggccc gccgcctcgt cgggtgagcg ggcaccctgt gggccgccgt
     8041 ttttctctgt ttcatattcc caactgccag gcgagatggt ggagccactc ccggctccat
     8101 ccgccgggga tggtgaggca gaaaaggagt atcgccatga agagcatcac cctgaaccag
     8161 atccccgggg tgaggcggga aggtattgcg gcgatgagcg acgccggcgc gaaacgcagg
     8221 gtgctgagct ccgtggtctt tctggagttc cttcgcgccg atctcgctat gagcaagagg
     8281 ccgatgaggg ccaccagagc gcctgaaaaa cggccgatgc cgaagtccct gaaaagagga
     8341 ccgggcgcct ggaagatgtc tatgaccaga tcgaacaccc cataccacaa catgaagtgg
     8401 ccgagaagcc tgccctgccc ctgggtgccc cctcgtccca cggcaaggag gatgaacatg
     8461 atgatgaggt ttttgaacgc gtcgtagagg gcaacgggat gccggaagcc ctcgacgagg
     8521 ggaaatttca ccgcccacca tgccgtggtc acgtaaccat agatctctcc gttcaggtga
     8581 ccgccgatgc gaacgagggc gaggaggaat gcgaagggta ttaccatctc gtcggccacg
     8641 agaaagaaac tcttcccgtg gacagcgcag aagaggaccg ctcctgccag cgctccgacc
     8701 atgactcctt catacgccat gccctccttc cagaaggcga agacctctgc gtatctgccg
     8761 ttgaagatat cccattcgta gacaaggacc tgaaaaactc gtccaaggac aaggcaggac
     8821 agggaaaaga cgagggaaag gtcccagacc tgcctcgaag tcagaccgac ataattgcgc
     8881 cggagcatca cccagaggag aacggccgag aatccgagag cccaggcaag cccataatac
     8941 cagagtctta ccccccagac ctcggcaagc aaaggatcca tcatcagaac aaaaggacct
     9001 tccaagtcga tcgcaacccc cgccgcgtgt tatcggacag aatgtttatt ttttctgtac
     9061 ttgtactact agcaacatat tcctgtcaac ttaaaagacg gttcaaagat gaagaaagtt
     9121 caagggttca agagttcaaa ggttcaagag taaaagaaaa aggcaccggt tgccttcggc
     9181 cggtgtgcac tagtcaatag ttgcgttgac aaccgccccc cttcgcggtc tttctcttga
     9241 actcttgaac cgcctttaag atgaacccct cccgttgcgg gagggaattg gggtagtgaa
     9301 aaaaatgggg taggggttta aggggtagtt atgactcgga tttctcgaaa gcgctcttcg
     9361 gggcgttaca gatgggacag gtccaatcct ccggaagctg ctcaaaagga actttctcga
     9421 ccttttcgtc gtactgataa ccacaaacgg aacatgtcca gaccatacga tccctcctct
     9481 attattggtt tgtttccatc attctttttt gctcgtcaga gcacgattta catatcccat
     9541 aaaaatctac gcggttgccg aggatctcga acccctgtgt ctgcgcatcg ggcagggtca
     9601 ggggaaactc gataaagacg tcaactatgg ccttgcactt gatgcacatg agatgatgat
     9661 ggggtttcgg attcggatca aaacgtttct tgccccggtc tgtggaaagc tcgatgacga
     9721 ccccctgctc tttcaaggcc tccattgtgt tgtaaacggt ggccagcgac atggtgggga
     9781 actggtccct gagggcccca tatatgtcgc ccgcgctggg gtggtacgtg ttgccgtcga
     9841 ggtacttcat aatagc
//
LOCUS       DAOPOA010000007         9253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_8435_length_9253_cov_3.845619, whole genome shotgun sequence.
ACCESSION   DAOPOA010000007 DAOPOA010000000
VERSION     DAOPOA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8435_length_9253_cov_3.845619"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1666)
                     /locus_tag="PKJ17_00280"
     CDS             complement(257..1666)
                     /locus_tag="PKJ17_00280"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD74427.1"
                     /translation="MIRVLIIDGGRQTCDMLAGCALSLGYDVSTGQTLTECLEKVTSN
                     PPDVIFIDGQLPDGSPLEVLDVIHAAPSSPTVIIMTEGGTPESAEMAIRNGAWDYIPK
                     PASMKEMTARLSRVAQYRRQHQGSERMAEIKRNGIVGNSPTMRTAMDMIKRAADSDLN
                     VLITGETGTGKELFALSIHLNSGRSQDNFVVVDCTALPETLVESILFGHSKGAFTGAD
                     RAQDGLIRQAHRGTLFLDEMGELPLPLQKTFLRVLQEHRFRPVGSQKEVESDFRLISA
                     TNKNLEQMVAEGKFRGDLLYRLGAMTIHLPPLRQHPEDIREMAVFYVEKVCRRFGVAP
                     KKFSDEFLKYLIRYDWPGNVRELFHVLEHACAAGLHEETLFPVHLPQELRITIARSLF
                     EDNHHVPRNGGNGSGSSAAKQILSLKRFRQSAYSDLEEMYLRNLLTSSAGSIKEACAL
                     SKLSRTRLYGLLKKYNIRH"
     gene            complement(1721..3541)
                     /gene="glmS"
                     /locus_tag="PKJ17_00285"
     CDS             complement(1721..3541)
                     /gene="glmS"
                     /locus_tag="PKJ17_00285"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOD74428.1"
                     /translation="MCGIVGYEGTREACDILIDALSRLEYRGYDSAGIALLGQGEIRI
                     ERRKGKVEELRKVLKGAALKGSIGLAHTRWATHGVPSERNAHPHRAGDVVVVHNGIIE
                     NYLELKEHLKGAGHVFISDTDTEVIPHLITDHARKGRDLVDSVRLALKELKGSWAVGI
                     LKESERTLVAAKNQSPLIVGVGDGEYFIASDIPAIINMTNRFIFLEDNDMAIFKGRDM
                     RIINVDGEDVVREVRQVCWTAGMAEKGGCSHFMLKEIFEQPQAISDTLLGRAREEEGE
                     VEFEGVDLPDPAGIRKIWMVACGTSHHACMIGKNLIETSLRIPVETDMASEFRYRDPI
                     VSPRDLFIAVSQSGETADTIAAMREARSRGASTLSICNVLGSTLAREAENVVFTHAGP
                     EIGVASTKAFTTQISVMFLLTLCIGKRLGIFGPQDVRERLREIRAIPRKVRSVLDEAP
                     SIQAAAARYMFFRSFLFLGRGINFPTALEGALKLKEISYIHAEAHAAGEMKHGPIALI
                     DDDMPVVCISPADRTYKKACGNMEEVMARKGNIILISDDETHEMAPRAGEMITVPRTA
                     YELQPILSIVPLQLLAYYIADLLGMDVDQPRNLAKSVTVE"
     gene            complement(4245..6452)
                     /locus_tag="PKJ17_00290"
     CDS             complement(4245..6452)
                     /locus_tag="PKJ17_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOD74429.1"
                     /translation="MERVTYYNEENHYTIAKVKIEGRNSQVTIVGTLYSLVPGEVLRL
                     KGSWEVHPRWGEQFKITSYETLMPATVKGIERYLGSGMIKGIGPVMAKRLVARFREAT
                     LDVIDTDMDRLREVPGIGQKRIDMIRTAWEEQKEVRNVMVFLQGNGVSPSYAAKIYRF
                     YGGDAVRMVRENPYRLAVDIFGIGFLTADSIAAKLGIGRDAPLRIETGILYVLGQLAS
                     EGHLFFPRDLLVERCAEVLEVDREKIPEALSNLAAQRKVVIDEPGQLSGGAVRYRDGI
                     VYLASLYVSEKGTAELLGTLHAHPRQMRLINVEEALRWVEGTQGVSFSPRQLEAVRAS
                     LEQKVLVITGGPGTGKTTIIRGIIAIAGKTGQKVLLAAPTGRAAKRMTEATGAEARTI
                     HRLLEYSPGNASSEGGFKRNESNPLDAGLIIIDEASMVDVSLMYHLLKAVPPPATLVL
                     VGDVDQLPSVGPGSVLKDIISSGCVRTVRLDEIFRQSTTSMIIVNAHRINAGEMPFFE
                     KHTGQGHDFFFVDIEDPDEVLRYVVRLCRDVIPTRFGLHPVSDVQVLTPMHRGVVGVS
                     NMNARLQEALNASTDGISRGGRFFKRGDKVLQTRNNYEKDVYNGDIGTVIAMDREVQE
                     LRVDFEGRVVSYDFNEVDELILAYAISVHKSQGSEYPAVVMPILTQHYLLLQRNLLYT
                     AITRGKKLVYLVGTKKALSIAIRNDKPHRRYTLLAERLRETKAVPGPRSHVPG"
     gene            6616..6960
                     /locus_tag="PKJ17_00295"
     CDS             6616..6960
                     /locus_tag="PKJ17_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74430.1"
                     /translation="MVRSGINVIQHQFHRMVANHKALLLHEAEKITGFMRLLMKPRNT
                     QAKWTKEERKLLKMHLKHLSYYVPALVIFVMPFGSLLIPLLAEILDRRKALRRPGDGL
                     GAPRSDTATLEP"
     gene            7175..>9253
                     /locus_tag="PKJ17_00300"
     CDS             7175..>9253
                     /locus_tag="PKJ17_00300"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOD74431.1"
                     /translation="MIRFNDIVDEVLKYNPDADVELLQKAYIFSAKAHKGQVRLSGEP
                     YLIHPLETAYTLTKMNLDIPSIVSGLLHDTIEDSYVDKKEIEEYFGKEIAELVDGVTK
                     ISKIPLKTSEESRVENFRKMILAMSKDIRVILVKLADRYHNMQTLNFLSAEKQIEIAR
                     ETFEIYAPLAHRLGIEWLRAELEDVAFKYLKPTEYRLIADNIAQKKREREAYINEVIG
                     LLRQKLDEFHVNADIFGRAKHLYSIYRKMSLEGLNIDDIYDIIAFRVVVDTIKECYEA
                     LGIIHAAYKPIPGKFSDYIALPKANMYQSLHTKIMGPHGEKVEIQIRTREMHRLAEEG
                     IAAHWKYKEGKVFNPKEDKIFAWLRRIIEWQQELKDNKEFMEIFKIDLFPDEVYVFTP
                     NGDVRELPKGATPVDFAYAIHSELGHRCVGAKVNGKLVPLRYTLKSGDTVEIMTSPTH
                     KPSKDWLSFVSTSKAKTKIRQWIKTEQREKSIELGRSLLDKELAKHSMSFSRLLKSGD
                     LLPLAKDFSFESVDDLFASVGYGLYTPLQVLNKIIPEEEKPAGKIKTIIHSLKKGRDN
                     AIKIKGIDGLVIRFARCCNPIPGDNIIGFITRGRGLTVHVEDCPNVHTYDEQRKVEVS
                     WQLSKDYTFPVRFKVTGNDRKGLLSDISSVIAANKVNILSASAMTYPDRSAAGIYEVE
                     IEHMSQLQKVM"
BASE COUNT         1916 a         2833 c         2567 g         1937 t
ORIGIN      
        1 cgtcggtctt tctgtgtgtt cagcatccac aatgactacc gtatatcaat aaccgtgcca
       61 ccagccctca cttttataaa gcgttgacag caaaggattt acgaagtacc gttgacggaa
      121 tcaccggggg cgtggaatga agatctgttc acttttccgg gacggcatcc aataaaactg
      181 gactctttgc ggtacactcc ggcggcatga ccggtgacgc cgggggttcc gggacgcggg
      241 gcgggtcacg cggggctcag tgccttatgt tgtatttctt gagaagaccg tacagtctcg
      301 tccgtgacag tttggaaagg gcgcatgctt ccttgatgct gcccgccgac gacgtcagga
      361 gattcctgag gtacatttcc tctaggtccg aataggcgct ctggcgaaac ctcttcaggc
      421 tcaggatctg ctttgccgcg ctggatccgg aaccgttgcc gccgtttcgc gggacgtgat
      481 ggttatcctc gaacagcgat ctcgcgatgg tgatgcgcaa ctcctgcggc agatgcacgg
      541 gaaagagcgt ctcctcgtgg aggccggccg cgcaggcgtg ctcgagaaca tggaacagtt
      601 ccctcacgtt ccccggccaa tcgtaccgta tgaggtattt caggaactcg tcggagaact
      661 tcttcggagc gacaccgaaa cggcgacaga ccttctccac gtaaaagacg gccatctccc
      721 ttatgtcttc gggatgctgt ctcagggggg gcaggtgtat cgtcatggcg ccgagacgat
      781 agaggaggtc gcccctgaat ttcccttccg cgaccatctg ctccaggttc ttgttcgtcg
      841 ccgatataag cctgaaatcg ctctccactt ccttctgact tccgacgggc ctgaagcggt
      901 gttcctgcag cacgcgaagg aacgtcttct gcagcggcag cggcaactct cccatttcgt
      961 cgaggaacag cgtcccccgg tgggcctgcc tgatgaggcc gtcctgcgcc ctgtcggcgc
     1021 cggtgaaggc acccttggaa tgcccgaaga gtatgctctc cacgagggtt tcgggaaggg
     1081 ccgtgcaatc gacgaccacg aaattgtcct gggacctccc gctgttcagg tggatcgaca
     1141 gggcaaagag ctccttgccc gtgcctgtct cccccgtgat gagaacattg aggtcactgt
     1201 cggcagcccg tttgatcatg tccatcgcgg tccgcatcgt gggactgttc ccgacgatgc
     1261 cgttgcgctt gatctccgcc atccgctcgc tgccctgatg ttgcctgcga tattgggcca
     1321 cacgcgacag gcgcgccgtc atctccttca tcgaggccgg cttcgggatg tagtcccagg
     1381 cgccgttccg tatggccatc tcggcgctct ccggcgtccc gccctcggtc atgatgatga
     1441 ccgtcggtga ggacggagcg gcgtggatca catcaaggac ctccaggggg cttccatcgg
     1501 gcagctgccc atctatgaag atcacgtcgg gaggattgga cgtgaccttc tccaggcatt
     1561 ccgtaagggt ttgtcccgtc gacacgtcgt accccaggct aagggcacac ccggccagca
     1621 tatcgcacgt ctgcctcccg ccatcgatga tcagcacccg gatcatggcc cgcggtctcc
     1681 ttccgagctc gcctggtgat atcttctccc gatcatggcg tcactccacc gtcacgcttt
     1741 tcgcgaggtt gcgcggctgg tcgacatcca tacccagaag gtccgcgata tagtacgcaa
     1801 gcaactgcag gggcactatg gagaggatcg gctgcagctc gtacgccgtg cgggggaccg
     1861 tgatcatctc ccccgcccgg ggcgccatct catgtgtctc gtcatcactg atcagaatga
     1921 tgttgccctt ccgcgccatc acctcttcca tattaccgca ggccttcttg taggtccggt
     1981 ccgccggaga gatgcacacg accggcatgt cgtcgtcgat gagggcgatg ggtccatgtt
     2041 tcatttcacc cgcggcgtgc gcctcggcgt gtatatagga gatctccttg agcttcaggg
     2101 ccccttcgag cgcggtcggg aagttgatgc cccttcccag gaaaaggaag ctcctgaaaa
     2161 acatgtatct cgcggcggcc gcctgtatcg acggcgcctc atcgagaacg cttcgcacct
     2221 tgcgggggat ggcccggatc tcgcgaagac gctccctgac atcctggggg ccgaagatgc
     2281 ccagtctttt accgatgcag agcgtgagca ggaacatgac ggagatctgc gtggtaaaag
     2341 ccttcgtgga ggccacgccg atctccgggc cggcatgcgt gaaaacaacg ttttcggctt
     2401 cgcgtgccag cgtgctcccg aggacgttgc atatcgacag cgttgaagca ccccgggacc
     2461 tcgcttccct catcgccgcg atcgtgtccg ccgtctcccc cgactgggag acggcaatga
     2521 agaggtcgcg gggcgacacg atgggatccc tgtatctgaa ctcggaggcc atgtccgttt
     2581 cgacgggtat tctcaggctc gtctcgatca gattttttcc gatcatgcag gcatgatggg
     2641 acgtgccgca cgccaccatc catatcttcc tgatgcccgc agggtcaggc aggtcgaccc
     2701 cttcgaactc aacctcgccc tcttcctccc gcgcccttcc gagcagtgtg tccgatatcg
     2761 cctggggctg ctcaaagatc tctttcaaca tgaagtgact gcatccgcct ttttccgcca
     2821 ttccggccgt ccagcatacc tgccggacct cccggacgac atcctcgccg tccacattga
     2881 tgatccgcat atcgcggcct ttgaatatgg ccatgtcatt gtcttcgagg aagatgaacc
     2941 tgttggtcat gttgatgatg gccggtatat cgctggcgat gaagtattct ccgtcaccta
     3001 cgccaacgat gagcgggctt tggttctttg cggccaccag ggtcctttcg gactccttca
     3061 ggatcccgac cgcccaggaa cctttgagtt ccttgagggc caggcggacg gagtcaacga
     3121 gatccctgcc tttccgggca tggtccgtaa tgagatgggg gatgacctcc gtgtccgtat
     3181 cggagatgaa aacgtggccg gcccctttca ggtgctcttt cagctcgaga tagttctcga
     3241 tgatcccgtt atggacgacc accacgtcgc ctgccctgtg aggatgcgcg ttcctttcgg
     3301 aaggcacccc gtgcgtcgcc cagcgcgtgt gggcgagtcc gatgctcccc ttgagagccg
     3361 cgcccttcag gaccttcctg agctcttcga ccttcccctt ccttctctct atgcggatct
     3421 ccccctgacc aagaagggct atccccgccg aatcgtatcc cctgtattca agcctgctca
     3481 acgcgtcgat gaggatatcg caggcctccc gcgtaccttc atatccgact attccacaca
     3541 tcgttccacc acctctcccg ccatgatccg gctcccccgg ataaacagat ccaaacctcc
     3601 cgcggagcct cgctcccgag aggaaccgaa gaattcccta cgagtacgct acaacggaca
     3661 tgacctcatg tctgtcgcct cagcgataat tatcttgtcg tactttctca gcgggaccgt
     3721 catcgtttgt tacgacaaat gcctcccccc ccgtcagtcc gctctcttca tcgccttgtc
     3781 cttcgttctg ccgccgagca gcccgcgggc gcaatccggc agataccatg ggtcaccgcg
     3841 acgcgtgata tctccgcatt caactctgac ctctcccaga caccgttcct cttccgctcc
     3901 ctgccgcacc aggcacagaa acagcgcgcc cgtcccgcgt atttcgtccc tcttccgatc
     3961 gtcataccat caccgtatct tctttgcccg ggccacctcc gctggtcacg cggtgttttc
     4021 ccccgggata tgtacaatct taaggaatgt cccgcgacct gtctgtcgcc gaagcgatga
     4081 ttccgatgtc ctgttttcgt agacggggaa agtgaatata tgatgacagg agagatcgtc
     4141 gcgggttccg ggttgcgggt tgcgtggtca aaggccaaga acggatggtt cttgcaaggg
     4201 ttccgatctt accggataac gcaggcatct ccggtttctt gtctttaacc tgggacgtga
     4261 gacctgggac ctgggactgc ctttgtctcc ctgagtctct ccgcgaggag tgtgtatctt
     4321 ctatggggtt tgtcgttgcg gatcgctatg gagagggcct ttttggtgcc gacgagatag
     4381 accagtttct tgccccgagt gatcgccgtg taaaggaggt tcctttgcag gagaaggtag
     4441 tgctgcgtca ggatgggcat caccacggcg gggtactcgc ttccctggga tttgtggacg
     4501 gatatcgcgt aggcgaggat gagttcatcc acttcgttga agtcgtagga cacgaccctt
     4561 ccttcgaaat ccactcggag ttcctgcacc tccctgtcca tggcgatcac cgtcccgatg
     4621 tctccattgt acacgtcctt ttcgtaattg ttccgcgtct gaaggacctt gtcgcccctc
     4681 ttgaagaacc tgcctccacg agagatgccg tccgtggagg cgttcaaggc ttcctggagc
     4741 cgtgcgttca tgttcgatac acccacgact ccgcggtgca tcggcgtcag gacctggacg
     4801 tcgctcaccg gatgaaggcc gaaacgggtg ggtatcacat cccggcagag acggacaaca
     4861 taccgcagga cctcgtcggg atcctcgata tcgacaaaga agaaatcgtg gccctgcccg
     4921 gtgtgcttct caaaaaaggg catctcgccg gcgtttatgc gatgggcgtt gacgatgatc
     4981 atgcttgtcg tcgactgccg gaatatctca tcgagcctta ccgtcctcac gcaccccgag
     5041 gagatgatgt ccttcaggac gcttccggga cccacggagg gaagctggtc aacgtcaccg
     5101 acgagaacga gggtcgcggg cgggggtacc gccttcagaa ggtggtacat gagggagaca
     5161 tcgaccatgg aagcctcgtc gatgatgata agccccgcgt cgagagggtt cgattcgttg
     5221 cgcttgaatc ccccctcgga cgatgcattc ccgggactgt actccaggag gcggtgtatg
     5281 gtcctcgcct ccgcccccgt ggcctctgtc atcctctttg ccgccctgcc ggttggagcc
     5341 gccagcagca ccttctgccc cgtcttgccg gcaatggcaa tgatccccct tattatggtc
     5401 gtcttacccg ttccgggacc tcccgttatg acaaggacct tttgctccag ggaagcccgg
     5461 accgcctcaa gctgccgcgg agaaaaagaa accccctggg tcccttcaac ccacctcaac
     5521 gcttcctcga cattgataag gcgcatctgc ctgggatggg cgtgaagcgt gccaaggagc
     5581 tcggctgttc ctttctccga aacgtagagc gatgcgaggt aaacgatccc gtcccggtac
     5641 ctcacggcgc ctccggagag ctgccccggc tcgtcgatga ccacctttct ctgtgccgct
     5701 agattcgaaa gggcttcggg gatcttctcc ctgtcgacct cgagcacctc cgcacatctc
     5761 tccaccagga ggtcgcgggg aaagaagagg tgaccttcgc tcgcgagctg tcccagcacg
     5821 tagaggatcc ctgtttcgat cctcagggga gcatccctcc cgatgccgag cttcgcggca
     5881 atgctgtcgg cggtgagaaa acctatgccg aatatgtcga ccgccaatct gtaaggattc
     5941 tcgcgcacca tcctgacggc gtcgccgcca tagaaacggt agatcttggc ggcataggac
     6001 gggctgacgc cgttgccctg gagaaagacc atgacgttgc ggacctcttt ctgctcctcc
     6061 caggccgtcc ttatcatgtc gatgcgtttc tgtcctatgc cgggaacctc gcgaagtctg
     6121 tccatatcgg tatcgatgac gtcgagcgtc gcttcgcgga accgcgcgac gagcctcttt
     6181 gccatgacgg gtcctatgcc ctttatcatg cccgaaccga ggtaccgttc gatccctttg
     6241 acggtggcgg gcatgagggt ctcgtaagag gttatcttga actgttcgcc ccaccggggg
     6301 tggacctccc agctgccttt gagccgaaga acctccccgg gaaccaggga atagagagtg
     6361 ccgacgatgg tgacctggga atttctcccc tctatcttca ctttcgctat ggtataatga
     6421 ttctcttcgt tgtaatatgt aactcgttcc acctgggccc gtatttcggg ctctgaagta
     6481 ttctcaaaca tgattaatag tatcggaaat gggccgataa taaaagtgaa acatgggccg
     6541 gggccattcg gccctcatgc ggtaggacaa aggcctggat gaccaccgcg cgtccgaggg
     6601 cgaagagaga accacatggt gcgttccgga atcaatgtga tacaacacca gttccacaga
     6661 atggttgcga accacaaggc actgcttctc cacgaagccg aaaagatcac cggtttcatg
     6721 cggctgctca tgaaaccgag aaacacccag gccaaatgga caaaggagga gaggaagctc
     6781 ctgaagatgc acctcaagca tctgtcatac tacgtccccg ccctggtgat attcgtcatg
     6841 ccctttggct ccctcctcat tccgcttctc gcagagatcc ttgacaggag aaaggccctc
     6901 cgcagacccg gggatggtct cggtgccccc cggtcagaca cggccaccct cgaaccctga
     6961 aacggggacc ttcccgagtc ccgcaatgcc gcgcgcttca ttacccgttg tcatttcgcg
     7021 ccgcataagg taatataagg gtcaggaaag acatgcgatc agatattccc cccgagcacg
     7081 gccaggaaag cagacgaaga gaacccgatc catccttctt tagagagacc tcccgcaacg
     7141 acgccccgct tgtcgaacac agacgggaaa acgcatgata cgctttaacg acatcgtgga
     7201 cgaggtcctg aagtacaacc ccgacgccga tgtcgaactg ctacagaagg catacatctt
     7261 ctccgcgaag gcgcacaagg gacaggtccg cctctccggg gaaccgtacc tcatacaccc
     7321 tctcgaaacg gcgtacaccc tgacgaagat gaacctcgac atcccgagca tagtctcggg
     7381 tcttctccac gacaccattg aagactctta cgtcgacaag aaagagatcg aggaatactt
     7441 cggcaaggag atagccgaac tcgtcgacgg tgtgacgaag atcagcaaga tcccactaaa
     7501 gacgtcggag gagtcccgcg tcgagaactt ccgcaaaatg atccttgcca tgagcaagga
     7561 catacgggtg atcctcgtca agctcgccga ccgctaccat aacatgcaga ccctgaactt
     7621 cctgtcagcg gaaaaacaga tcgagatcgc cagggaaacc ttcgagatat acgcgcccct
     7681 ggcacacagg ctgggtatag agtggctcag ggcggagttg gaggacgtgg ccttcaagta
     7741 cctcaaaccg accgaataca ggctcatcgc cgacaacata gcccagaaga agagggaacg
     7801 cgaggcctac atcaatgagg tcatcgggct tttgcgccag aaacttgatg agttccatgt
     7861 aaacgccgac atattcgggc gggcgaagca tctctacagt atctaccgca agatgtcact
     7921 ggaggggctc aacatcgacg acatctacga catcattgcc ttccgggtgg tcgtcgacac
     7981 gatcaaggag tgctacgagg cgctcggcat catccacgcc gcctacaagc cgataccggg
     8041 aaagttcagc gactatatcg ccctcccgaa ggccaacatg taccagtctc tgcacaccaa
     8101 gatcatgggt ccccacggcg agaaggtcga gatacagata aggaccaggg agatgcaccg
     8161 cctggcggag gaagggatcg cggcccactg gaaatataag gaaggcaagg tcttcaatcc
     8221 caaggaggac aagatcttcg cgtggctgcg ccggatcatc gagtggcagc aggaactcaa
     8281 ggacaacaag gagttcatgg agatcttcaa gatcgacctc ttccccgacg aggtatacgt
     8341 cttcacgccg aacggcgacg tgagagagct ccccaaggga gcgactcccg tcgatttcgc
     8401 ctacgcgatc cactcggagt tgggtcaccg gtgcgtcggc gccaaggtga acggaaagct
     8461 ggtgcccctt cgctacacct tgaagagcgg cgacaccgtg gagatcatga cgagccccac
     8521 ccacaagccg agcaaggact ggctgagctt cgtatcgacc tccaaggcga agacaaagat
     8581 caggcagtgg ataaagacgg aacagcggga aaagagcatc gagcttggcc gcagccttct
     8641 cgacaaggaa ctggccaagc attcgatgag cttctcccgg ctcctcaaga gcggcgatct
     8701 cctgcccctt gccaaagatt tcagcttcga gagcgtcgac gatctctttg ccagcgtggg
     8761 ctatgggctg tacactccgc tgcaggtact caacaagatc atccccgagg aggaaaaacc
     8821 ggcggggaag atcaagacca tcatccactc actgaagaag gggcgggaca acgcgataaa
     8881 gatcaagggg atagacgggc tcgtgatacg tttcgcgcga tgctgcaatc ccatccccgg
     8941 cgacaacatc atcggtttca taacccgggg tcgcggcctc accgtccacg tcgaggattg
     9001 cccgaacgtc cacacctacg acgagcagcg caaggtggag gtgagctggc aactcagcaa
     9061 ggactacacc ttccccgtga ggttcaaggt gacgggaaat gaccgcaagg ggctcctctc
     9121 cgacatctcg agcgtcatag ccgccaacaa ggtcaacatc ctcagtgcct cggcgatgac
     9181 ctatcccgac agatcggcgg cggggatcta cgaggtcgag atcgagcaca tgtcacagct
     9241 gcagaaggtc atg
//
LOCUS       DAOPOA010000008         8933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_8790_length_8933_cov_3.825636, whole genome shotgun sequence.
ACCESSION   DAOPOA010000008 DAOPOA010000000
VERSION     DAOPOA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8790_length_8933_cov_3.825636"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PKJ17_00305"
     CDS             complement(<1..1297)
                     /locus_tag="PKJ17_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD74432.1"
                     /translation="MKNGHWMTAKDVLRVNAFKWPDKVGIKDLYKAYTFKQWNERACR
                     LANALADLGMKKGDRFAVLGYNCVEWLEFYAAAAKGGFLCVPLMFRLAAPEMEYIINH
                     CEAKVFIVQSGIDKADGKEFPWINQVNGMKKNLKTVEHYINFAVDSPSYDGFINYEQM
                     LAKASAAEPDVVVDADDIWVIMYTGGTTGLPKGVMKSHANLFSQYFIMIYDHSFNFDD
                     CNLLVMPCCHVNSLFYSFVVTWVGGTVMAYNMVSFNPEDLIKTFADFGVTFTSLVPTH
                     YIMMLALPDEVKAKYNLNAIKKLLCSSAPARRDTKQGILKMFPNSALYEAYGSTEAGI
                     VTILKPHEQMTKLGSIGREVVGTDILQLYDEDGNLIDKPNVVGELYSRSPQIFEQYWK
                     DPEKTKAAMKGEYFSAGDMAMRDEEGYYYLVDRKANMIIS"
     gene            complement(1816..3423)
                     /locus_tag="PKJ17_00310"
     CDS             complement(1816..3423)
                     /locus_tag="PKJ17_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD74433.1"
                     /translation="MKNGHWMTAKDVLRIQAFKYPDKVGAKDLYKQFTFKEWNERTCR
                     LANALADLGLKKGDRFAVLAYNCVEWMDFYGAAAKGGFLCVPLMFRLAAPEMEYIINH
                     CEAKAFIVQSGKDPEDGKEFPWIEQVNGMKKNLKTVEHYINFAVDSPSYDGFINYEQM
                     LAKASPAEPDVEVTPDDTWVIMYTGGTTGLPKGVMKSHANLFSQYFIMIYDHEFNYDD
                     TILLVMPCCHVNSLFYSFVVTWVGGTVMCYNMVSFNPESLIKCFSDHGITFTSLVPTH
                     YIMMLALPDEVKAKYNLNAIKKLLCSSAPARRDTKQGILAMFPNSRLFEAYGSTEAGC
                     VTILKPEEQMKKLGSIGREAIGTDIIQIYDEDGKLITEPNKIGELYSRSPMIFESYWK
                     DPEKTKNAMKGQYFSAGDMGYRDEEGYYYLVDRKANMIISGGENVFPSEVENCVGSHP
                     DIKDVAVIGIPHEKWGEQVTAVVVLHEGKKMEPKEVTEWCKGKIAGFKVPKNIYIIPD
                     EEMPRSGAGKILHRILRERYGKWGDQQ"
     gene            complement(3486..4757)
                     /locus_tag="PKJ17_00315"
     CDS             complement(3486..4757)
                     /locus_tag="PKJ17_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOD74434.1"
                     /translation="MKDLKELKKIEEFSNQCMKCGFCSFFCPVYQEEKMETSVARGKN
                     YLVKQVLAGKQEFTHEMADIIGKCLLCKRCAANCPAKTQIDRVVVAARAQLVKEKGLG
                     AIKNFAFRKVMADRKAFGKYARLMQKFQWMMPTTEGKIKHLPDFVKGLGKRNFPSLAK
                     TFLRDQIKPVYKPQNGEKPKMRVGFFMGCATDFVYPELGLKLIDFLTRHGVEVVVPED
                     QNCCGAAIYFSGDLETGRMLADLNIKAYKDLNVDYIVTACATCSSTLKDYQKYLPDTE
                     EQKERYTEFEKKIKDSTEFVIDILKIPPEEFKLRKEFEGKTVTWHDPCHLVRYQNIKD
                     QPRVILKALKGIKYVEMPNADMCCGMGGSFSVYHYDLTKKIAGKKMDGIKATGADIVV
                     TACPGCMINLIDNVLLNKMPQKVHHFLELVE"
     gene            complement(4777..6159)
                     /locus_tag="PKJ17_00320"
     CDS             complement(4777..6159)
                     /locus_tag="PKJ17_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOD74435.1"
                     /translation="MLKESLISEFQKIVGKDNVLTSPEALKAYSYDGTTSWVHEPDVV
                     IFPTSTDEVSRIMKLANAEKIPVTPRGGGTNVSGGSVPWHGGVVLVMTKFNQILKVDK
                     ENLTATVEPGVVLQDLTLRLAKEGLFFPPDPQSFLGATMGGIISENAGGPAGVKYGVT
                     KQYVLGVEVVLPSGEVIHLGGRTLKNVVGYDLLHIFISSEGTLGVITKAELKLNPLPQ
                     ARKTLCVVYDDVSIAGEGVFRVLENGVIPCKIEMVDNWVINRIEEMMPIGLPKDADAC
                     LFFEVDGIPEVVEKEAQKIAEITKQYGAKEARIAPNMDEANKYWLARRAGFAAVFGKA
                     KQVFAEDVTVPRGNIPKFVKKIRELAKKYDVEIVVLGHAGDGNLHPAVLTDPANKDHY
                     ERALKAVDEIIESAAVEFGGVLSGEHGIGLEKQKFMRKVTEPAVINMMKGIKALIDPN
                     NIMNPGKIWE"
     gene            6389..7303
                     /locus_tag="PKJ17_00325"
     CDS             6389..7303
                     /locus_tag="PKJ17_00325"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOD74436.1"
                     /translation="MDILETIAPIFIIIAFGYVLKSRKMLSDAFVSEANRFVFRFPLP
                     FLIFTGILKANMKDVGWLSVLSLIIPTAMVMALAALSAVVARYQGGKRGSFIQTTFHG
                     NVTYVGLAVIFYLLGDEGLERGSLLVGMLILFNNAGAVLALSLSSGKHRNVMKTALSI
                     LTTPVIVATFAALILAFLKVTMPAVIMKSITIVANIALPMALIIIGCSISLEMLRKTL
                     QPAALAATLKVIVLPALAVLFCRIFAIDLKEALPAIILLATPTAITAYIMARELGGDT
                     DLASGTVTLTTLVSPLTYLGWIWLLKTA"
     gene            complement(7442..8257)
                     /locus_tag="PKJ17_00330"
     CDS             complement(7442..8257)
                     /locus_tag="PKJ17_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOD74437.1"
                     /translation="MHFGVSTYFLTNDSLDGVIDSIIAAGIGVVELSYEPPHLFSLDG
                     ALADKVRRLAAEGIGFSMHGPFLEMNLGSYLDEIRQVSRERMLSALRLSARLGAEPVV
                     VHPGYSFFRKLKDYDRELRARFIEDLGTIQEEARSLGIRIALENVFMSYFYFQELDEF
                     AAISEAVPGIGVTLDIGHAYISKCAAKHPDPEGAIIDDVRRLGVENLFHVHLHNNSGS
                     RDDHDFVEGSIDMARVLKGLTDLGYAGKVVIETLDIERLGFPAVLQKLEQIAP"
     gene            complement(8392..>8933)
                     /locus_tag="PKJ17_00335"
     CDS             complement(8392..>8933)
                     /locus_tag="PKJ17_00335"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOD74438.1"
                     /translation="NAVKAGSELGKKAKGYMERGELVPDDLIMDIMEVRMKEPDCQKG
                     FLLDGFPRTIPQAEALKKLLDKIGMKLDAAINLDVPTDVILDRLTTRRTCSNPDCQEI
                     YNIKSKPPKPDGTCFKCGSPAVQRADETEEAIKQRLATYNEKTAPLIDFYKKEGLLKT
                     VSSLNSSEIVEEIKKAVKK"
BASE COUNT         1794 a         2526 c         2371 g         2242 t
ORIGIN      
        1 cggagatgat catgttggcc tttctgtcga cgagatagta gtagccttcc tcatccctca
       61 tggccatgtc gccggcgctg aagtattcgc ccttcatcgc tgccttggtc ttttccgggt
      121 ctttccagta ctgctcgaag atctgggggc tccgtgaata gagttcgccg actacattcg
      181 gtttgtcgat gaggtttccg tcttcatcat agagctgaag gatatcggtg ccaacaacct
      241 cgcggccgat ggaaccgagt ttcgtcatct gttcgtgcgg tttcaggatg gtaacgattc
      301 ctgcttccgt ggaaccatag gcttcgtaca gcgccgagtt ggggaacatc ttgaggatgc
      361 cctgcttcgt gtcgcgccgc gccggtgccg aggagcacag aagcttcttg atggcgttga
      421 ggttgtattt cgccttcacc tcgtccggga gtgccagcat catgatgtag tgcgtcggga
      481 cgagggaggt gaaggtgacg ccgaaatccg cgaaggtctt gatgaggtct tcggggttga
      541 aggataccat gttgtaggcc atgacggtcc cgccgaccca ggtcacaaca aatgaataga
      601 acagggagtt gacgtggcag cagggcatga caagaaggtt gcagtcgtcg aagttgaagc
      661 tgtgatcgta gatcatgatg aaatactggg agaagaggtt ggcatggctc ttcataacgc
      721 ccttgggcag gcccgtggtg ccgccggtgt acatgatgac ccagatatcg tcagcgtcaa
      781 cgacaacatc cggctctgcc gcgcttgcct tcgcgagcat ctgctcgtaa ttgatgaagc
      841 cgtcatagct ggggctgtct accgcgaagt tgatgtaatg ctcaacggtc ttcaggttct
      901 tcttcatacc gttcacctgg ttgatccagg ggaactcctt gccgtctgcc ttgtctatcc
      961 cgctctgaac gataaagacc tttgcttcgc agtggttgat gatgtattcc atctccgggg
     1021 cggcaagcct gaacatgagg ggcacgcaca ggaatccgcc ttttgccgcg gccgcgtaga
     1081 actccagcca ctcgacacag ttgtatccga ggactgcgaa acggtcgcct ttcttcattc
     1141 ccaggtcagc aagggcgttt gcaagtctgc aggccctttc attccactgt ttgaaggtgt
     1201 aggccttgta aaggtccttg atgcccacct tgtcgggcca tttaaacgca ttcaccctca
     1261 acacatcctt agccgtcatc caatgaccgt tcttcataac aaaaccctcc ttagtgtgat
     1321 tgatgaaaat cccgttatgt agccctgccg acagctcagc ggggcatcgt gctcacaggc
     1381 aggcagtacg tgcgacactg caacaacata caatccagta gtgaatatgg aattgtgccg
     1441 tcagaaaaaa gatcagctat ggaaacagcg cctctacagc ttctcacgta accccttaac
     1501 ccgaataaaa ccttgtacac catcttttac cccacacggg ttttttttgc aatactaaaa
     1561 tgccgccatt acccgcatga gtgcaataat tcattcccgc agtttccggc cgatccctgt
     1621 ctgccaggag aggctatagg acctatagga aataagacct ataggacata taagacccat
     1681 aggacgtgtt gaaggcaatc gcccacaatc gacccgtcct ataagtccta agcgtcctat
     1741 aagtcctatt ggtcctatcc ccaacccaaa aaaaacgggg accgaagtcc ccgtttttgg
     1801 ttcagttgga aaatcctact gctggtcgcc ccacttgccg taacgctcgc gcaggatcct
     1861 gtggaggatc ttgccggcgc ccgatctcgg catctcttcg tcggggatta tgtatatgtt
     1921 cttcggaacc ttgaaaccag cgatcttgcc cttgcaccac tcggtgactt ctttgggctc
     1981 catcttcttg ccttcgtgga gaacgacgac tgccgttacc tgttcgcccc atttctcgtg
     2041 gggaataccg atgacggcca cgtccttgat gtcggggtgt gagcccacgc agttctcaac
     2101 ttccgacggg aagacgttct cgccgccgga gatgatcatg ttggcctttc tgtcgacgag
     2161 atagtagtag ccttcctcat cgcggtagcc catgtcgccg gcgctgaagt actggccctt
     2221 catcgcgttc ttcgtctttt ccgggtcttt ccagtagctc tcaaagatca tggggctgcg
     2281 ggagtagagc tcgccgatct tgttgggctc ggtgatgagc ttgccgtctt cgtcgtagat
     2341 ctggatgatg tccgttccaa tggcctcgcg cccgatggaa ccgagtttct tcatctgctc
     2401 ttccggtttc aggatcgtca cgcagcctgc ctcggtcgat ccgtatgcct cgaagagtct
     2461 cgagttcggg aacattgcga ggataccctg tttggtgtcg cgccgcgccg gtgccgagga
     2521 gcacaggagc ttcttgatgg cgttgaggtt gtatttcgcc tttacctcgt ccgggagtgc
     2581 cagcatcatg atatagtgcg tcggcacgag ggaggtgaag gtgatgccgt gatccgagaa
     2641 gcacttgatg aggctctcgg ggttgaatga gaccatgttg tagcacatga cggtgccgcc
     2701 gacccacgtc acgacgaagg agtagaagag tgagttgacg tggcagcagg gcatgacgag
     2761 cagaatggtg tcgtcgtaat tgaactcatg gtcgtagatc atgatgaaat actgggagaa
     2821 caggttggcg tggctcttca tgacgccctt gggcaggcct gtcgtgccgc cggtgtacat
     2881 gatgacccag gtatcgtcgg gggtcacttc gacatccggc tccgcggggc tcgccttcgc
     2941 cagcatctgc tcgtagttga tgaagccgtc atagctgggg ctgtcgacgg cgaagttgat
     3001 gtaatgctca acggtcttga ggttcttctt catgccgttg acctgttcga tccaggggaa
     3061 ttccttgccg tcctcgggat cctttccgct ctggacgata aaggcctttg cttcacagtg
     3121 attgatgatg tactccatct ccggtgccgc aagccggaac atgagaggca cgcaaaggaa
     3181 gccgcccttt gcggccgcgc cgtagaaatc catccattcg acgcagttgt aggcgagaac
     3241 cgcgaaacgg tcgcccttct tgagacccag atcggcaaga gcattcgcga gccggcaggt
     3301 cctctcattc cactctttga aggtaaactg cttgtaaagg tctttcgctc cgaccttgtc
     3361 agggtactta aatgcttgaa tccttagaac atccttcgct gtcatccagt ggccgttctt
     3421 catgtgtata ctcctcctgt gagtttgaga tgtagatgta cggttgtcct agggatatcg
     3481 atctgctact ctaccagctc caggaaatga tgcaccttct gcggcatttt gtttagcaaa
     3541 acgttatcaa taaggttgat catgcagcct gggcaagcgg tcacgacgat atccgcaccg
     3601 gtcgctttga tgccatccat tttcttgccc gctattttct tggtcagatc gtaatggtac
     3661 acgctgaatg aaccacccat tccacaacac atatcggcat taggcatttc cacgtattta
     3721 atacccttga gggccttgag gatcaccctc ggctgatctt tgatgttctg gtacctgaca
     3781 agatggcagg ggtcatgcca cgttactgtc ttcccttcga actcctttct caacttgaat
     3841 tcttccggag ggatcttgag gatatctatg acaaactccg tactatcctt gatcttcttc
     3901 tcgaattccg tgtagcgttc tttctgctcc tcggtatccg ggagatattt ctggtaatcc
     3961 ttcagcgtgg aactgcacgt agcgcaggcg gtgacgatat aatccacgtt gaggtccttg
     4021 taagccttta tgttgaggtc tgccagcatc cggcccgttt cgaggtcgcc gctgaaatat
     4081 atcgcggcgc cacagcagtt ctggtcctca ggcacaacca cttccactcc gtggcgggtc
     4141 aggaaatcga tgagcttgag acccagctcg gggtaaacga agtccgtcgc gcagcccatg
     4201 aagaaaccga ccctcatctt cggcttctcg ccgttctggg gtttgtagac cggcttgatc
     4261 tggtctctca ggaacgtctt cgccaggctg gggaagttcc tcttcccaag gccctttacg
     4321 aaatcgggaa ggtgtttgat cttgccctcc gtcgtgggca tcatccactg gaatttctgc
     4381 atgagcctgg catacttccc gaaggccttc ctgtcggcca tcaccttgcg gaaggcgaag
     4441 ttcttgatgg ctccgagccc cttctccttc accaactgcg cgcgggcggc cacaacgacg
     4501 cggtctatct gcgtctttgc cggacagttc gccgcgcagc gtttacagag gaggcacttc
     4561 cctatgatgt ccgccatctc gtgcgtgaat tcctgctttc ccgccagtac ctgtttcacg
     4621 aggtagttct ttccgcgcgc cacagacgtt tccatcttct cttcctggta tacagggcag
     4681 aagaaactgc agaaaccaca cttcatgcac tggttggaga attcttctat ttttttaagt
     4741 tctttcaggt ctttcactcg caaccccctt tatggactac tcccagatct tgcccgggtt
     4801 catgatgttg ttcgggtcaa taagtgcctt gatccctttc atcatgttta tgacggccgg
     4861 ttcggtgacc ttcctcataa atttctgttt ctcgaggccg ataccgtgtt ctccggagag
     4921 tactccacca aactcaacgg cggcgctttc gatgatctca tccaccgcct ttagggcgcg
     4981 ctcataatgg tccttattgg caggatctgt caaaacggcc ggatggaggt ttccgtcacc
     5041 ggcatgaccg aggacgacga tctcgacatc atatttcttg gccagttccc tgatcttctt
     5101 gacgaacttg gggatatttc cgcgtggtac cgtgacgtct tccgcgaaca cctgcttggc
     5161 ttttccaaat acagcggcga aaccggcacg ccgggccagc cagtacttgt tggcctcgtc
     5221 catatttggc gcgatgcggg cttcttttgc accatactgc tttgtgatct ccgcgatctt
     5281 ctgcgcttct ttctcgacga cctcagggat cccgtcgacc tcgaagaaga ggcatgcgtc
     5341 ggcatccttc ggcagaccga tcggcatcat ctcttcgatc ctgttgatga cccagttatc
     5401 gaccatttcg atcttgcagg gaataacgcc attctcgagc acgcggaaaa caccttcgcc
     5461 agcaatcgag acatcatcat agacaacgca gagtgttttt cttgcctgag gcagggggtt
     5521 caatttcaat tcggctttgg tgataactcc gagtgttccc tcggagctga tgaaaatatg
     5581 aagcaggtcg taaccaacga cattcttcag cgttctgcca ccgagatgaa tgacctcgcc
     5641 gctcggaagg accacctcga cacccagaac atactgtttt gtgacgccat atttcacgcc
     5701 ggcaggaccg cctgcattct cggatatgat accgcccatc gtcgcaccga ggaaactctg
     5761 cgggtccggc gggaagaaca aaccttcctt cgcgagccgg agggtgaggt cctggaggac
     5821 aacgcccggc tcgaccgtgg cggtcaggtt ttccttgtca accttgagta tctgattaaa
     5881 cttcgtcatg accaggacaa cgccgccatg ccagggaaca gaccctccgc tgacgtttgt
     5941 gccgccgccc ctgggggtta cgggtatctt ttcagcgttg gccagcttca tgatccggga
     6001 cacttcatcc gtagacgtag ggaagatgac gacatccggc tcatggaccc agcttgtcgt
     6061 tccgtcgtag gaatacgctt tcagagcctc tggcgaggta aggacgttgt ccttgcccac
     6121 aatcttttga aattcgctta tcaacgactc ttttaacatc ccttctaccc cctgagtttg
     6181 atattgttac taatttcact ttcttatatc cttttcccct ttcctttgtc aacaaaaatc
     6241 acaaggccaa aaatgctctg atatcaagta attggccaca gttaaggcct tgcggaagaa
     6301 tgggaaaaat ggtatggaat cgtatgtttc ccatagtgaa caaatggacc gcacatcgcc
     6361 tgtacatcga cgttttctgg gggtatacgt ggatatcctt gagaccatag ctccgatctt
     6421 catcatcatc gccttcgggt atgtcctgaa gagcaggaag atgctttccg atgccttcgt
     6481 ttcggaggcg aaccgtttcg tgttccgatt ccccctcccc tttctcatct tcacggggat
     6541 cctgaaagcg aacatgaagg acgtgggatg gctgtcggtc ctctccctca tcataccgac
     6601 ggccatggtt atggccctgg ccgccctttc ggcggtagtg gccaggtacc agggcggcaa
     6661 acggggaagt ttcatccaga cgacgttcca cggcaacgtg acatacgtcg gccttgccgt
     6721 tatcttctat cttctcgggg acgaaggact cgaaaggggg agcctcctcg tcggtatgct
     6781 gatactcttc aacaatgccg gagccgtgct ggcgctctcc ctgtcttccg ggaaacaccg
     6841 caacgtgatg aaaacggcgt tgtcgatcct caccacaccg gtcatcgtcg ccaccttcgc
     6901 tgccctcatc ctcgccttcc tgaaagtgac catgcctgcc gtcatcatga agagcatcac
     6961 gatcgtggcc aacatagccc ttcccatggc actcatcatc atcggctgct ccatttctct
     7021 ggagatgctc aggaagaccc tgcaaccggc cgctctcgcc gcgaccctca aggtcatcgt
     7081 ccttcccgcg ctggcggtcc tgttctgccg tatcttcgcg atagacttga aagaggccct
     7141 tccggcgatc atcctcctcg cgacaccgac ggccatcacg gcctacatca tggcacgtga
     7201 gctcggcggc gacaccgacc tcgcctccgg cacggtgaca ctgacaaccc tcgtctcacc
     7261 cctgacgtat ctggggtgga tatggctctt aaagacggct tgaatcgctt taattgtttg
     7321 agagtgaaag gcaaaaagat ctcccggtgg ggtccggtgc gtcgcacggc gaggatcctc
     7381 ttgcctgctc cttctcaacc ctcaagcgac tcaagcggtt caagccgttc aagcgcgccc
     7441 gtcagggcgc gatttgttcc agtttttgga ggactgccgg gaagccgagg cgttctatgt
     7501 ccagggtctc gatgacgacc ttgccggcgt agcccaggtc cgtgagaccc tttaagaccc
     7561 tcgccatgtc tatcgaaccc tccacgaagt cgtggtcgtc gcggctgccg ctgttgttgt
     7621 gcaggtggac atggaagagg ttttccacgc ccaatcggcg gacatcgtcg atgatcgcgc
     7681 cttcggggtc gggatgcttc gccgcgcatt tcgagatata ggcgtggccg atgtcgaggg
     7741 tgacccctat cccgggaacg gcctcactga tggcggcgaa ctcgtccagt tcctggaagt
     7801 agaaatagga catgaacacg ttctccagcg ctatcctgat cccgagggac ctggcctctt
     7861 cctgaatggt gccgaggtcc tcgatgaatc gggccctcaa ctccctgtcg taatccttga
     7921 gcttgcgaaa gaaggagtat cccgggtgga ccacgaccgg ctcggcaccg agtcgggcgg
     7981 agagtctgag ggcggagagc atcctttccc tggagacctg ccgtatctcg tcgaggtagc
     8041 tccccaggtt catctcaagg aaggggccgt gcatggaaaa cccgatcccc tccgcagcca
     8101 ggcgccgtac cttgtccgcc agcgcgccgt cgagggagaa caagtgggga ggctcatagg
     8161 aaagctcgac gaccccgatg ccggcggcga taatactatc gatcacaccg tcgaggctgt
     8221 cgttggtgag gaaatatgtg gatacgccga aatgcatgat agaggcgggt tatgggtaat
     8281 aggtcatggg ttatgggaac aaccggaaaa caagagacgc gatgtccgac agcatgcgca
     8341 acgcctccgg ggtctttttc ccatcaccca taacctgtat tctttgtatt cttacttctt
     8401 caccgccttc ttgatctctt ccacgatctc cgagctgttc agggaactga cggtcttcag
     8461 aagcccttcc ttcttgtaga agtcgatgag gggggcagtt ttttcgttgt atgtggcaag
     8521 cctctgcttg atggcctcct ctgtctcgtc ggcgcgctgc accgcgggag agccgcactt
     8581 gaagcaggtc ccgtcgggtt tcggcggctt gctctttatg ttgtagatct cctggcagtc
     8641 agggttggag caggtccgcc tcgtcgtcag cctgtcgagg atgacatccg tcgggacgtc
     8701 gaggttgata gcggcgtcaa gcttcatgcc gatcttatcg aggagcttct tcaatgcctc
     8761 ggcctgggga atggtcctcg ggaaaccgtc gagaaggaaa cccttctggc agtcgggctc
     8821 cttcatccgg acttccatga tgtccatgat gaggtcgtcg ggcacgagct ctccccgttc
     8881 catgtagccc ttcgccttct tgcccagttc ggaccccgcc ttgacggcgt tgc
//
LOCUS       DAOPOA010000009         8634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_9134_length_8634_cov_3.207950, whole genome shotgun sequence.
ACCESSION   DAOPOA010000009 DAOPOA010000000
VERSION     DAOPOA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9134_length_8634_cov_3.207950"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="PKJ17_00340"
     CDS             complement(<1..1629)
                     /locus_tag="PKJ17_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD74439.1"
                     /translation="MRRLFLAALVFLFFFPLCAAAQECIDCHEKYQRVDHAKLKCVAC
                     HSDAKDLPHAEKLKKPECASCHRDAVREHGTSVHAGKGLTCKSCHNVHTPRGQTKRCV
                     DCHTSVAHRKLPSARKHLAEMNCAGCHAKTAQGHIDVKVESKQMVTRDVLDKDGNKSV
                     DEKEWKDFLAHAQSVVGNGFRIKRVYSATGSAHSIGPRAVSCNGCHVENKVFRKAMLE
                     MNTGGQRTRIALDLHSVIPRLPIVDTYRLTAHGKGGVACSDCHVSQKRIDDHVCAKCH
                     DKVYGVYKGTAHAKGGATKCTDCHDPHKVKTYRELGAAERVAVCARCHGDYIKQHRWL
                     PHAELHFMYLECSTCHSPLSKKSMVFHINLQTKDGKRRFTRDDITAAFRESKYTRDLI
                     DVNGDERIAPSEIIPFFEAMERTTKGTVGIEGSIVVTDIHHDYSQVQKRDKVCTTCHS
                     NDAPFYQSMYLAVPEMEGLFYMPVKGTVLAAMPSSIALNFFLLGETKARWSDITALVG
                     ARGEVRDEIVKELGFKWIDILGIFLSLAVLFFVCI"
     gene            1772..2035
                     /locus_tag="PKJ17_00345"
     CDS             1772..2035
                     /locus_tag="PKJ17_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74440.1"
                     /translation="MAELSQSDLDALFADLGTPEAPQPALEQAKAHEPAPQPDLDKLM
                     ADLEALGVNTTPEPQQTAPVSSLPDNESLSQDQIDALLKEFLG"
     gene            complement(2149..2649)
                     /locus_tag="PKJ17_00350"
     CDS             complement(2149..2649)
                     /locus_tag="PKJ17_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOD74441.1"
                     /translation="MRVTAREGKRSAGLLMYRRRGGAVEVLLIHPGGPFWSGKDDGAW
                     SIPKGLIDPGEDGLLAAIREFEEETGFPAEGEFVALPPLRQPSGKVIAAWAFEGDCDP
                     ADMKSNLFSMEWPPRSGRMAEFPEADRAAWFDIPSAKTKIIKGQKGFIEELERLLSIN
                     SHRTFR"
     gene            complement(2778..3689)
                     /locus_tag="PKJ17_00355"
     CDS             complement(2778..3689)
                     /locus_tag="PKJ17_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOD74442.1"
                     /translation="MVKLYNIVESRVTECVGAAGNIAVYINPDENERRRLIDQDQIDE
                     HTLQSALDPDELSRIEIESNHVAIILKVPVNYQAEHAFEFLVSSMGMFLFENKLVIVM
                     AEDLPIFDSKTFARVSRLAETMLKIVNYSIFRFLEHLRVIDMVSDELGEKISTSMENT
                     YLLNLFALEKSLVYYQNAINSNSVLIEKMKIYTQRIGFTVDDTELLDDITIENTQCYK
                     QAEIYSNILASMMDARVSIVNNNLNILMKKLTMITIAIMVPTFVVSAFSMNVAIPVQK
                     HPQAFWIILAIAFAAMFGFFFIWRMRK"
     gene            3888..4121
                     /locus_tag="PKJ17_00360"
     CDS             3888..4121
                     /locus_tag="PKJ17_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74443.1"
                     /translation="MKKLLLASTLLVLIAAVVIVSPPAASASSCREDCLSDCCTDSRC
                     TKEKEARCYEDCLRDCNSAATKLKKDKSTLPPT"
     gene            complement(4205..5488)
                     /gene="eno"
                     /locus_tag="PKJ17_00365"
     CDS             complement(4205..5488)
                     /gene="eno"
                     /locus_tag="PKJ17_00365"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOD74444.1"
                     /translation="MSTIYDVYAREILDSRGNPTVEVEVVLESGAMGRAMVPSGASTG
                     EREALELRDGDAKRYKGKGVKKAVENVNNIIAPEVEGLDALDQAYIDKVLVDADGTEN
                     KSSLGANATLGVSMAVTRAAADFLGVELYQYIGGIRGREIPVPMMNVINGGAHAQNSL
                     DVQEFMIVPAGAPSFSEALRMGAEVFHTLKGILKDKGYSTGVGDEGGFAPQIESTTEA
                     LDTLMTAIAKAGFKAGKDIFLALDVAASELYKKGKYHIDGNVWDAGKLVDFYEAIVKK
                     YPIVSIEDGFAQNDWKGWKKFTDRCASTIQIVGDDIFVTNPGIFAEGIGKGIANSILI
                     KLNQIGTVTETLTTIEMAKRAGYTCVISHRSGETEDTFIADLAVATNVGQIKTGSASR
                     SERIAKYNQLLRIEDELGELAIFGGTGVFYSVRKK"
     gene            5716..6105
                     /locus_tag="PKJ17_00370"
     CDS             5716..6105
                     /locus_tag="PKJ17_00370"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HOD74445.1"
                     /translation="MARKTLVYYLIAVMFIIGIAPRVDAAFLPSETIGITAAQRDADI
                     QRIQAVLEMKLVRQRLLDLGFSPEEIATRVSEMTDEQVHSFAQKLDDLRVGKDSGLGI
                     VIAVLIIVLLVILIINLTTGKKVVVTQ"
     gene            6153..6713
                     /locus_tag="PKJ17_00375"
     CDS             6153..6713
                     /locus_tag="PKJ17_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOD74446.1"
                     /translation="MAELLPMKTLPFLCAMLLLFLGCTSGPALLPEGARVLSGVPFFP
                     QEDYRCGPAALATVMNYWYARSGSAVRTSIDEIAQAIYSPGARGVLPTDLENYPRKKG
                     FVTEQFTGSIGRLRENIDRGIPVILLVDHGLSVYRLDHFLVATGYTGDGIIVNSGREE
                     RQVVSNSELEKAWKKTGYWALRLFPS"
     gene            6668..7135
                     /locus_tag="PKJ17_00380"
     CDS             6668..7135
                     /locus_tag="PKJ17_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD74447.1"
                     /translation="MEKDRLLGASALSLLTAGLLFCLCSCSLPRILVLNDPLTPQEHI
                     NLGVTYERSGELDEALKEYEKASRTLPVAHLYMGNVFFQKKEFAKAEEHYREAIDRTG
                     DPGAYNNLAWLYYSTDKNLDEAEKLARKAVELAPDKKEFKDTLQKIREKTMTN"
     gene            7285..7614
                     /locus_tag="PKJ17_00385"
     CDS             7285..7614
                     /locus_tag="PKJ17_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOD74448.1"
                     /translation="MKKQNVFSPIPSDIPDEIFETIAASGTVRIERIVSRGQATPQGS
                     WYDEELAEWVILLKGSADILFEGEATAVTLLPGDHILIPPHRRHRVTRTDDEAPTIWV
                     AVYFADT"
     gene            7763..>8634
                     /locus_tag="PKJ17_00390"
     CDS             7763..>8634
                     /locus_tag="PKJ17_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD74449.1"
                     /translation="MADEYTRERALEMLGATDGALEELYRHADAVRKNHMGDDVYIRG
                     IIEFSNICVNDCLYCGIRASNRNVARYVMSPDEILAIAGTMGRFRQTTVVLQSGETPG
                     IADEKLGVLIRRIKDATGLAVTVSVGNRPLETYRYWRECGMDRYLLRFETSDAALFAR
                     LHPGSSLGDRLRCLEDLKGLGVQTGSGFMIGLPGETVGVLADNILLCRRLDLDIAAEA
                     LLQRGLDRLQLGGGQRDSGGHLGLHHALRLEAQLIEHAGDFRQQRQAAVLEQQAQEVA
                     EHRVGTFRRQQRRG"
BASE COUNT         1815 a         2607 c         2360 g         1852 t
ORIGIN      
        1 gatacagaca aagaacagta ccgcgagaga gaggaagatc ccgaggatgt cgatccactt
       61 gaaccccagt tccttcacga tctcgtctcg cacttcaccc cgtgccccga caagcgccgt
      121 tatgtcgctc caccgcgcct tcgtttcgcc gagaagaaag aaattgaggg cgatcgaaga
      181 gggcatggcc gcgaggaccg tcccttttac cggcatgtag aaaagaccct ccatctccgg
      241 taccgcaagg tacatggatt gataaaaagg agcgtcattg gaatgacagg tcgtgcagac
      301 cttatccctc ttctgtacct gcgaatagtc atggtgaata tcggtgacga caattgagcc
      361 ttcgatcccc accgtgccct ttgtggttct ctccatcgct tcgaaaaagg ggatgatctc
      421 cgatggagca atgcgctcat caccgttcac atcgataagg tccctcgtgt acttcgactc
      481 ccggaaggcc gccgtgatat catcgcgcgt gaaccgcctc ttgccgtcct tcgtctgcag
      541 gttgatgtgg aagaccatgc ttttcttcga gaggggactg tggcaggtgg agcattcgag
      601 gtacatgaaa tggagctccg cgtggggaag ccaccggtgc tgctttatgt agtccccgtg
      661 gcagcgcgcg cagaccgcca cacgctccgc tgcgccaagc tcccggtatg tcttcacctt
      721 gtggggatcg tgacagtcgg tgcacttcgt cgctcctccc ttagcgtgcg ccgtcccctt
      781 gtacacaccg tacaccttgt catgacattt cgcgcagacg tgatcgtcta tgcgcttctg
      841 ggatacgtga caatcactgc atgccactcc ccccttgccg tgcgcggtga ggcgatacgt
      901 gtcgacaatg gggaggcggg ggataacgct gtggaggtca agggcaattc ttgtcctctg
      961 cccgccggtg ttcatttcca gcatcgcctt ccggaacacc ttgttctcga catggcaacc
     1021 attgcaggaa accgccctgg ggccgatgga atgagcgctg cctgtcgcgg aatagaccct
     1081 ctttatcctg aaaccattcc ccacaaccga ctgggcatgg gccagaaaat ccttccattc
     1141 cttctcatca acggacttgt tgccgtcctt gtcgagaaca tcccgcgtca ccatctgctt
     1201 cgattccacc ttcacatcta tatgtccctg cgcggtcttc gcatggcacc ccgcgcagtt
     1261 catctccgca agatgctttc tcgccgacgg cagtttcctg tgcgcgacgg aggtatgaca
     1321 gtcaacacat cgctttgttt gcccccgcgg ggtatgaaca ttgtgacagg atttgcacgt
     1381 caggcccttg ccggcatgca cgctcgtccc gtgctccctg acggcatccc ggtggcagga
     1441 cgcgcactcg ggtttcttca gcttttccgc gtgaggcagg tccttcgcgt cgctgtgaca
     1501 ggcaacacat ttcagcttcg catggtccac cctctgatac ttctcatgac aatcaataca
     1561 ctcctgagct gcggcacaca gcggaaagaa aaaaagaaag acgagtgctg cgaggaaaag
     1621 acgtctcatt attttgttca ctccgctgta taatacctcc aaaagactcc aaatgcaatc
     1681 gcccgaccac gatctccacg atttttcctc tatatttcta tcgtcctgcc gataatactt
     1741 catagcccgc caggggcgtt agaggacacc tatggctgaa ctttcacaat ccgatctcga
     1801 cgcgctcttt gccgacctcg gaacccccga ggcgccgcag cccgccctcg agcaggcaaa
     1861 ggctcatgag cccgcaccgc aaccagacct cgacaagctc atggccgacc tcgaagcact
     1921 cggcgtcaac accacacccg aaccccagca aaccgcaccc gtaagctccc tccctgacaa
     1981 cgaaagcctc tcccaggacc agatcgacgc cctgctgaag gagttcctgg ggtagaaaca
     2041 ggtttcaggc aaagatacgc agaacctcta aaacaaggtg atctccggag ggttcgaatg
     2101 tgcggcttga ggctgacggc ttgtgacagg ctggcgatgg atcacgagtt accggaaagt
     2161 ccggtggctg ttaatactca ggaggcgctc cagttcttcg atgaagcctt tctggccctt
     2221 gatgatcttt gtcttcgccg acgggatatc gaaccaggcg gccctgtcgg cttccgggaa
     2281 ttcggccatc ctgcctgaac gcggcggcca ttccatggaa aaaaggttgc tcttcatgtc
     2341 cgcggggtca cagtcgccct cgaaggccca cgcggctatc accttcccgc tcggctgcct
     2401 gagcggtggc agtgcaacga actcgccttc cgccgggaag cccgtttcct cctcgaattc
     2461 ccgtatggcc gccaggaggc catcctcgcc gggatcgatc aaccctttcg ggatactcca
     2521 ggctccgtca tccttaccgg accagaaagg cccgcctgga tggatgagca gcacctcgac
     2581 cgcacccccg cgccgacgat acatcaacaa tcccgcgctt ctttttcctt cccgtgcagt
     2641 cacgcgcatc tcccttatct ctctattgta gctgaaaaca acacgaccat ccagcaaaga
     2701 ggcgacagaa ggattccgcc ggacccgttc ttaacgttca agcggctcaa gccattcaag
     2761 cggctcaagc cgtctcccta cttcctcatc ctccagatga agaagaagcc gaacatggcc
     2821 gcgaaggcta tggcgaggat tatccagaac gcctggggat gcttctggac gggaatggca
     2881 acgttcatgg aaaaggcgct gacgacgaag gtcgggacca tgatggcgat ggttatcatg
     2941 gtgagcttct tcatcaggat gttgaggttg ttgttcacga tggaaaccct ggcatccatc
     3001 atgctcgcga gaatgttgga atagatctcg gcctgcttgt agcactgggt attctcgatg
     3061 gtgatgtcgt cgaggagctc cgtgtcgtcg acggtgaacc ctatccgctg cgtgtatatc
     3121 ttcatcttct cgatgaggac ggagttggag ttgatcgcgt tctgataata cacaaggctc
     3181 ttctccagcg cgaagaggtt gaggaggtac gtgttctcca tggaggtgct gatcttctcg
     3241 cccagttcgt cggagaccat gtcgatgacc ctcaggtgct ccaggaagcg gaagatgctg
     3301 tagttgacaa tcttcagcat cgtctcggca agacggctga cacgggcaaa ggtcttgctg
     3361 tcgaagatgg gcaggtcttc cgccatgact atgacgagct tgttctcgaa gaggaacatg
     3421 cccatggatg aaacgaggaa ctcgaaggca tgctcggcct gataattgac aggcaccttg
     3481 aggatgatgg cgacgtgatt cgattcgatc tcgatacggg aaagctcatc ggggtcaagt
     3541 gccgactgca gggtgtgctc gtctatctgg tcctggtcga tgagacggcg tctctcgttc
     3601 tcatcggggt tgatgtagac ggctatgttt cccgcggcgc cgacgcattc cgttacccgt
     3661 gattccacta tgttgtagag ctttaccata cgacataccg ttcatgtttg gattgatacg
     3721 tacaatgtat cccatcttct cggaaaaagc accgaaattc tcaacaaaag atgcctcctg
     3781 tccatcgagg tcgcctcccc atcccgcctc aagtgtgaca caggacattg actgtcaaaa
     3841 accatagtgt tataaatcgt catgccccat atcaaggagg tcacacgatg aaaaaacttc
     3901 ttctcgcatc aaccctgctg gtcctcattg ccgccgtggt catcgtatcg ccgcccgcgg
     3961 cgagcgcgtc gtcatgtcgc gaggactgcc tctccgattg ctgcactgat tcccgatgca
     4021 ccaaggaaaa agaggccagg tgctacgagg attgcctgag ggactgcaac agcgcggcca
     4081 cgaaactcaa gaaagacaaa tcgacactgc ctcccacgta acggaaaagg accagcgccc
     4141 ccttcacagg cgggcgccgg tccctgatat tcctgccgcc gatagaaccc ttatcctgga
     4201 acccttactt cttcctgacg ctgtaaaaaa cgcccgtgcc gccgaatatg gccagttcgc
     4261 cgagttcatc ctcgatcctg agaagctgat tgtacttcgc aatcctctcg cttctcgagg
     4321 cggaccccgt cttgatctgg cccacgttgg tcgcaaccgc aaggtcggct atgaaggtgt
     4381 cctcggtctc gccggaccgg tgtgagatga cacaggtgta acccgcccgc ttggccatct
     4441 cgatggtcgt caatgtttcc gtgacggtgc cgatctgatt gagcttgata aggatgctgt
     4501 tggctatccc cttcccgatc ccctcggcga agatccccgg gttcgtcacg aagatgtcgt
     4561 ccccgacgat ctgaatggtg gaggcacatc ggtcggtgaa tttcttccag cctttccagt
     4621 cattctgggc gaacccgtcc tctatggaga caatggggta cttcttcacg atcgcctcgt
     4681 agaaatccac cagcttgccc gcgtcccaca cgttgccgtc tatgtggtat tttcccttct
     4741 tgtaaagctc gctcgcggcg acatcgaggg ccaggaagat gtccttcccc gccttgaaac
     4801 cggccttggc tatggccgtc atgagcgtgt cgagcgcctc cgtggtcgac tctatctgcg
     4861 gggcgaagcc accttcgtcg ccgacgcccg tcgagtatcc cttgtccttc aggataccct
     4921 tgagggtgtg gaacacctcc gcgcccatcc tcagggcttc gctgaatgac ggcgcgccgg
     4981 cggggacgat catgaactcc tgcacatcaa gggagttctg ggcatgggcg ccgccgttga
     5041 tgacgttcat catgggaaca ggtatctccc gccccctgat ccccccgatg tactggtaca
     5101 gctcaacccc cagaaagtcg gccgcggccc gcgtgacggc catcgaaacg cccagggtgg
     5161 cgttggcacc gaggctgctc ttgttctcgg taccgtcggc atcgacaagg accttgtcga
     5221 tgtaggcctg gtcgagggca tcgagtccct cgacttccgg ggcgatgatg ttgttcacgt
     5281 tctcgacggc cttcttcacg cccttgccct tgtacctctt cgcatcgccg tcgcgcagct
     5341 caagggcttc cctttcaccc gtggaggcac ccgagggtac catcgcgcgc cccatcgcac
     5401 cgctttccag gacgacttcg acctcgacgg tgggatttcc cctgctgtcc agtatttccc
     5461 gcgcgtatac atcatatatc gttgacatga gaacctcctt gttttgtaag gggaccggcc
     5521 tcacaaacca ccggccgata tgaatgcagg ataccacagg agtcgatgtc cttcaatggt
     5581 catatgttgt tacgaacgcg gctcaccgga atgcgggcct tcgcggcagg cctcaaaagg
     5641 gtttacaaac cgcggtatat cggatatata ctcttacata cgatcagtcg ggtcgatctg
     5701 aaaggaggtg acggcatggc cagaaagact ttggtttatt atctcatcgc agtgatgttc
     5761 atcatcggca ttgccccccg tgtcgatgcc gcgttcctcc cgtcggagac gatcggcatc
     5821 accgcggcac aaagagacgc cgatatccag aggatacagg cggtcctcga aatgaaactc
     5881 gtgaggcaga ggcttctcga tctcggtttc tctccggaag agatcgcgac acgggtctcc
     5941 gaaatgacgg atgagcaggt ccacagtttc gcccagaagc ttgacgatct gagagtgggc
     6001 aaggatagcg ggctcggtat cgtcatagct gttctcatta tcgtactgct cgtcattcta
     6061 atcatcaacc tcaccacggg caagaaggtc gtggtgacgc aatagcggag cattcgcgac
     6121 aaaagcgcca cggggtgcct ttcggccgcc ccgtggccga actcctcccc atgaagaccc
     6181 tgccgttcct gtgcgccatg ctccttctct tcctgggctg cacctccgga cccgccctgc
     6241 tgcctgaagg ggcgagggtc ctttccggtg tccccttctt cccccaggag gactatcggt
     6301 gcggtccggc cgccctcgcg acggtcatga actactggta tgcccgcagc ggcagcgccg
     6361 tcaggaccag tatcgatgaa atagctcagg ccatctacag ccccggggcg cggggcgtcc
     6421 tgcccacgga cctcgagaac tacccgagaa agaagggctt cgtgacggag cagttcacag
     6481 gttccatagg ccgcctgcgc gagaacatcg acaggggcat ccctgtcatc cttcttgtcg
     6541 accacggcct gtccgtctat cggctcgatc acttcctggt ggcgacgggc tataccgggg
     6601 acggcatcat cgtgaattcg ggccgcgagg agagacaggt cgtttccaat tccgaactgg
     6661 agaaagcatg gaaaaagaca ggttactggg cgcttcggct ctttccctcc tgacggcagg
     6721 tctcctcttt tgcctctgct cctgcagcct tccgaggata ctcgtcctca atgacccctt
     6781 gacaccacag gagcatataa acctcggggt gacctatgaa aggtcgggag aactcgacga
     6841 agcgctgaag gaatacgaaa aagcctcgag gaccctgccc gtcgcccatc tctacatggg
     6901 caacgtcttc ttccaaaaga aagagttcgc gaaggccgag gaacactacc gcgaggcgat
     6961 cgacaggacc ggcgaccccg gagcatacaa caacctcgcc tggctctatt attcaacgga
     7021 caagaacctc gacgaggcgg aaaagctcgc acgaaaggcc gtcgagctgg caccggacaa
     7081 aaaggaattc aaagacactt tgcagaaaat aagggaaaag acaatgacca attgaccaga
     7141 cagaagggta ggaggggccc atgtaaaagt taagatcttc ccggatacct ttggtcattg
     7201 atcattgtct gtttttgtca ttggaatttg atcattggtc attattagct tcattggtaa
     7261 ttgtcttctt ttatcgagga taccatgaaa aagcagaacg tcttctcccc catcccgtcc
     7321 gacattcccg acgagatatt cgagaccatc gccgcgagcg ggaccgtcag gatcgaaagg
     7381 atcgtctccc gcggccaggc gacgccgcaa ggatcctggt acgacgagga gcttgcggaa
     7441 tgggtcattc tcctcaaggg aagcgccgac atcctcttcg agggcgaggc aaccgccgtc
     7501 acccttcttc cgggggacca catcctcatc ccgccgcatc ggcgccacag agtgacacga
     7561 accgacgacg aggcgcccac gatatgggtt gcggtgtatt ttgctgacac gtgaagatgt
     7621 gtcaaacatg aacgggcaca aggatcgcgc gcttcacgcg cgacggggcg gacggggaga
     7681 agccctgttg ccggctcacg cgtcgtcgat atgacgcctc cccggttgca aagacgctct
     7741 cttatcgggt atattgtcag ccatggcaga tgaatacaca cgggaacgcg ccctcgagat
     7801 gcttggcgcg accgacggag cgctcgagga actctaccgt cacgccgacg cggtgcggaa
     7861 gaaccacatg ggagatgacg tctacatacg ggggatcatc gagttctcaa acatatgcgt
     7921 caatgattgc ctctactgcg ggatacgggc ctccaacagg aatgtcgccc gttacgtgat
     7981 gtctcccgac gagatactgg cgatagccgg tacgatgggc cgcttccgtc agaccaccgt
     8041 tgtcctccaa tcgggcgaga cgccggggat cgccgatgag aagctggggg tccttataag
     8101 acggatcaag gatgcgacag gtctggccgt taccgtttcc gtgggcaaca ggcccctcga
     8161 gacataccga tactggcgcg agtgcggcat ggaccgatat cttctccgtt tcgagacgtc
     8221 cgacgcggcg cttttcgcgc gcctccatcc gggatcgagc ctcggtgacc ggctgcgctg
     8281 cctcgaagac ctcaagggcc tcggggtgca gacgggcagc ggattcatga tagggctccc
     8341 cggtgagacc gtgggagtgc tggcggacaa catcctgttg tgccgccgtc tcgacctcga
     8401 catcgcggcc gaagcgcttc ttcaacgcgg ccttgatcgt ctccagctcg gcggcgggca
     8461 gcgcgacagc ggaggtcacc ttggccttca ccacgcgctc cgcctcgaag cgcagctcat
     8521 cgaacatgcc ggtgatttcc ggcagcagcg acaggcggcg gttctcgaac agcaggctca
     8581 ggaagttgcc gaacatcgcg tcggcacctt ccggcgccag caacgtcgtg gcat
//
LOCUS       DAOPOA010000010         8440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_9394_length_8440_cov_3.880382, whole genome shotgun sequence.
ACCESSION   DAOPOA010000010 DAOPOA010000000
VERSION     DAOPOA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9394_length_8440_cov_3.880382"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PKJ17_00395"
     CDS             <1..560
                     /locus_tag="PKJ17_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOD74450.1"
                     /translation="SALRAFLRQDPDIMLVGEVRDLETAEICVRSALTGHLVLSTLHT
                     NDAPSAVTRLIDIGVEPYLLAPTVLAVVGQRLVRKLCPECKEAYRPTTKDLGSVKLNA
                     DTIYRAAGCPACNNTGYRGRTLIAEIMSSSEELKSLITHTVSYQNMRETARKLGMTTL
                     YESGLKKVEKGITSLEEVMSVTFGI"
     gene            complement(681..1145)
                     /gene="greA"
                     /locus_tag="PKJ17_00400"
     CDS             complement(681..1145)
                     /gene="greA"
                     /locus_tag="PKJ17_00400"
                     /inference="COORDINATES: protein motif:HMM:NF001261.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOD74451.1"
                     /translation="MKVPITREGYEALKKEHETMLSVKRPQILKAIEEAREHGDLSEN
                     AEYDAAREEFQFLQQRVAEIEEQLRNSEIVDVRKGDGETVEFGCTVTLVNLDTDEESL
                     YRIVGPYESDIKKSTISITSPLGRALMGKSVGEEINFNAPGGKRTYEIVKVD"
     gene            1365..1805
                     /gene="mraZ"
                     /locus_tag="PKJ17_00405"
     CDS             1365..1805
                     /gene="mraZ"
                     /locus_tag="PKJ17_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOD74452.1"
                     /translation="MFAGRYEYSIDDKSRVSIPAKFREALGQRYDLRLILTNLDGCIV
                     AYPYQEWLAIQDKVSAAGTIRKEARAFLRYFYSGASECPIDKLGRILIPQALKTDAHI
                     QKNVVITGQGKKIEVWAKEKWEELVRLATADPDQIADIVSELGL"
     gene            1802..2671
                     /gene="rsmH"
                     /locus_tag="PKJ17_00410"
     CDS             1802..2671
                     /gene="rsmH"
                     /locus_tag="PKJ17_00410"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOD74453.1"
                     /translation="MSNQEHIAVLSREVCDNLIGTGCKVFVDATVGGAGHSIGLLERY
                     RQLQIIGIDRDKEALDRAEGTLKGYSNRVTLLRGNFRDLKQILGGIGVTSIDGILFDL
                     GISSYQLAAGRGFSFNDDEELDMRMDTRDVLTAYEIVNSYRQDEIARILYEYGEEWQS
                     RRIAKAIFDSRRKGRISTARELAGIVSSVKRKTGKIHPATKTFQALRIAVNDELGSLS
                     EGMDAAVDLLSRSGRIGMITFHSLEDRMVKQRFRGDAALTVLTRKAIRPGMDEIRSNP
                     RSRSAKLRVAEKN"
     gene            2682..2993
                     /locus_tag="PKJ17_00415"
     CDS             2682..2993
                     /locus_tag="PKJ17_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74454.1"
                     /translation="MHRPKKIYIESSGDALFSSVRPFVFLGFIMVFVLVVSYFLVSAH
                     NRAREDLIETLTMERELRQTHKKLATEMAGITQSRLLALKAKERLGLTKPKEEEVLVL
                     K"
     gene            2990..3838
                     /locus_tag="PKJ17_00420"
     CDS             2990..3838
                     /locus_tag="PKJ17_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74455.1"
                     /translation="MNKQAIRKAFFVCAFIGLAYAALAFNVQDRFSMKRTYETSAKKA
                     PRSLNVAVPGPQKEALRQAPVIAGDDLSFEPSLLIAIPIVAWSIREGIVEKDGMVLVK
                     MTEDPSSVYLKKPLDILKDRDKEGLRALARIIGRKNMETFLTREGVKLPDRSLSFDTL
                     AGTGYSVHGRTLTALYNKYVDAGFDPLMPYASGGLEVSRKAGGFTIGKVREERKAQAD
                     PDSAAWTMPDLGGLSMKAALDRISTKGQAVRIYGSGVVTDQYPKPRERVEKDTRCILY
                     GRTYQK"
     gene            3835..5286
                     /locus_tag="PKJ17_00425"
     CDS             3835..5286
                     /locus_tag="PKJ17_00425"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOD74456.1"
                     /translation="MRLSELIRGMKKTAVTGDTSLEITGVTKDSRNVSEGSLFFATST
                     SSAYLNDALERGARAVVTDGDIDRTFACVIRVEDVKRALGEAASKFHGHPSRKMHVTG
                     ITGTNGKTTTTYLIESIFEAAGKRPGVIGTISYRYAGKTLNAPNTTPGASETNALLRD
                     MMDAGISHVIMEVSSHALHQSRVEGIDFDVAVFTNLTHDHLDYHGDFESYKASKRLLF
                     EDFLARSRKKRRYAIMNIDDPSIRDFAVAPPVTMLSYSTRGFADACLSSYSESIDGLT
                     LTLSLPGKKIPIVSPLVGMFNASNILAASLSGHVTGISPQAIKEGIERLGGVPGRLER
                     VANDRGISIFVDYAHTPDALENVLSLLGRLKKGRLIVVFGCGGNRDAVKRPLMGAIAA
                     RFADEVIVTSDNPRNEDPLTIIEDIRRGLEGKAARIIPDRREAIFEGIGSARPDDVVL
                     VAGKGHEDYQIIGGTTLHFSDREVIEESLHVAT"
     gene            5273..6631
                     /gene="murF"
                     /locus_tag="PKJ17_00430"
     CDS             5273..6631
                     /gene="murF"
                     /locus_tag="PKJ17_00430"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOD74457.1"
                     /translation="MWRLEDIVKAVGGTVLKKEKEVFAAISTDSRTIGKGDLFIPILG
                     KSFDGHAFIGEALKRSGGGTLCRADSPVSLADADGTVILVEDTNQALLDLARFKRHTL
                     SGAFISITGSNGKTTTKEILAAMMTKRYGVHFNEKNLNNLIGVPMSILSVAGDPEICI
                     LELGTNTPGEIKKLAVATNPDASLITNVNPSHLEGLASLEGILEEKLDLFRHTKEGGK
                     IFVNIDDPGIAGWWRTTQRTTVTFGIDSDADFRLAVGEDLGWEGSDVIISCPAGTIRA
                     RTSLLGRHNLYNILAAAAIASTMGVEGELIGEAIGKFVSYDKRFQPATSPKGYVIIDD
                     TYNANPSSMKWAISTLAALPAAGKRVAVLGGMKELGEMSARYHRELGAYLREAGLGLI
                     VLLGEETKDTMAELGGAPAAHFDNKEELIGYVASQAGPGDTILVKGSRAFRMEDIVEA
                     LK"
     gene            6631..7707
                     /gene="mraY"
                     /locus_tag="PKJ17_00435"
     CDS             6631..7707
                     /gene="mraY"
                     /locus_tag="PKJ17_00435"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOD74458.1"
                     /translation="MLYYLLYQLHMKVSGFNVFRYITFRTVLSILTALIISFILTPYV
                     IRKFHDWKIRSGKREDVPDRHEAKKETPTMGGFVILIATMIPTLLWADLTNYYIWVVS
                     FSFVSFGAIGFMDDVKKLKGEKGKGISGRMKLSLQIFFGLCIGALIFFKYGFVTQLTI
                     PFFKNLRPDLGVFYIALCIFIIAGTSNAVNLTDGLDGLAIGPVLTICSTFMLFSYLVG
                     NVIFAQYLQIFYVKGAGELTVLCGAMLGAGIGFLWYNAHPAELFMGDTGSLSLGAALA
                     TIAVIIKQEFLLVIAGGIFVLETLSVIIQVYSFKLRGKRVFRMAPIHHHFELKGWNEE
                     KIVVRFWIISVILGLIALSTLKLR"
     gene            7709..>8440
                     /gene="murD"
                     /locus_tag="PKJ17_00440"
     CDS             7709..>8440
                     /gene="murD"
                     /locus_tag="PKJ17_00440"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOD74459.1"
                     /translation="MDIPDRILIVGLGASGTAAARFLSRKGKTIGLADEKNAAELAGP
                     LTDLEGIPFTGHFGPHRAEDFASYPLIVLSPGVDSELPVLRRAREAGARVIGEMELAA
                     SFIEEPIIAITGTNGKTTTTTLIGEVFSRAFGGVFVGGNIGNPLINYVAEGKKTSHVI
                     VEVSSFQLETIETFRPATAVLLNITEDHLDRYRSYDEYRQAKYRIFDNQGSGDSAVIR
                     KGLDVVMKGSPRVLSFSVTEELDEGA"
BASE COUNT         1924 a         2483 c         2456 g         1577 t
ORIGIN      
        1 cgtcggcctt gagggccttc ctgcgacagg accccgacat catgctcgtt ggcgaggtcc
       61 gcgacctcga gacggcggag atctgcgtcc gctcggcgct gacgggtcat ctggtgctct
      121 ccacgctcca caccaacgac gccccgtcgg cggtgacaag gctcatcgat atcggcgtcg
      181 aaccctacct gctcgcaccg acggtccttg ccgtcgtcgg tcagagactc gtgaggaagc
      241 tctgccccga atgcaaggaa gcctacagac ccaccacgaa agacctgggc tccgtaaagc
      301 tcaacgcaga tacgatctac cgggctgcgg gatgcccggc ctgcaacaac acaggttatc
      361 gtgggcgcac actgatcgcc gagatcatgt cctcctcgga ggagctcaaa tcgctcatca
      421 cgcacactgt ctcctaccag aacatgcgcg agactgcacg caaactgggc atgaccaccc
      481 tctacgaatc aggcctcaaa aaggtcgaga aaggaataac gagcctcgaa gaagtaatga
      541 gcgtgacatt cggaatctaa agacggcttg agtcgcttga atggcttgag tcgcttgaga
      601 gtaaaaaaag agaccttctc caatgaagag atggtctttt gttttcaaca ttcaagcggc
      661 tcaagccatt caagccgcct ttagtctact ttcactattt cgtacgttct cttgccaccg
      721 ggggcgttga agttgatctc ttcaccgacg gacttgccca tgagggccct gccgaggggt
      781 gaggttattg atatggtgct tttcttgatg tccgattcgt aggggccgac gatcctgtaa
      841 agggattctt catcggtgtc gagattgacg agggtcacgg tgcagccgaa ctcgaccgtt
      901 tctccatcgc ctttcctcac gtcaacgatc tcggaatttc tcagttgctc ttcgatctcg
      961 gcgaccctct gctgaagaaa ctgaaattct tccctggcgg catcgtattc cgcgttctcc
     1021 gaaaggtcgc catgctcccg cgcttcctct atggccttga ggatctgggg ccgttttacg
     1081 ctgagcatgg tctcatgctc tttcttcagt gcttcatagc cttccctggt aattggtacc
     1141 ttcattgacg ctccgattat tcgcgatgga gtaaaagact acctgaggcg gggcaaatta
     1201 tcaacagatt tgtgtcagca tccaccccac ctcttctgac accctcgggg actggcccga
     1261 aagagaagcg ccagacggcg cagatggggg aaaaaaacct tgacaagata tggagttaca
     1321 agtataaatg actaaagtgg taaaaggtgt aaaaaggtgg gagattgttc gcaggacgct
     1381 acgaatattc gatcgatgac aaaagcaggg tgagcatccc ggcgaagttc agggaagccc
     1441 tggggcagag gtacgacctc aggctcatcc tcaccaacct ggatggctgt atcgtcgctt
     1501 atccctacca ggagtggctc gccatccagg acaaggtttc cgccgcgggg actatccgaa
     1561 aggaggcgcg ggcttttctg cgttatttct actccggggc atcggagtgc cccatcgaca
     1621 agctgggacg gatcctcatc ccccaggcgt tgaaaacgga cgctcacatt cagaagaatg
     1681 tcgtcatcac cgggcaaggg aagaagatag aggtctgggc aaaggaaaaa tgggaggaac
     1741 tggtaaggct ggccacggcc gaccccgatc agatagctga tatcgtatcg gaactcggtc
     1801 tttgagcaac caggaacaca tagccgttct ttccagagaa gtatgtgaca acctgatcgg
     1861 caccggatgc aaggtcttcg tcgacgccac tgttggcgga gcgggacatt ccatcggctt
     1921 gctggaaaga tacagacaat tacagatcat cggtattgat agagataagg aagcactcga
     1981 ccgggcagag ggaaccctca aggggtatag caaccgggta acactactga ggggaaactt
     2041 cagggacttg aagcagatac tgggggggat cggtgtgacg tccatagacg gcatcctctt
     2101 cgatctcggt atatcaagct accagctggc tgcagggcgt ggcttcagct tcaacgacga
     2161 cgaggagctc gacatgagaa tggacacgag ggatgtcctc accgcatatg agatcgtgaa
     2221 ctcctaccgg caggacgaga tcgcccggat cctctatgaa tacggggagg aatggcagtc
     2281 gcgcagaata gcgaaggcca tcttcgacag ccgcaggaag ggcaggatca gcacggcgcg
     2341 ggagctggcc ggcatcgttt cctccgtcaa gagaaagacg gggaagatac accccgccac
     2401 gaagacattc caagcgctcc gcatcgccgt caacgacgag ctggggagcc tttcggaagg
     2461 gatggacgcg gccgtggacc tcctttcccg cagcggcagg ataggcatga taacattcca
     2521 ttccctggaa gaccgcatgg tgaaacagcg tttccgcggc gacgccgcgc tgaccgtgct
     2581 gacacggaag gccatcaggc cggggatgga cgagatacga agcaacccgc gctcgcgcag
     2641 cgccaagctt cgtgttgcag aaaagaactg acgggggccc tatgcacaga ccaaagaaga
     2701 tatacatcga gtcatcgggg gacgcgctct tctcgagcgt cagacccttc gttttcctcg
     2761 ggttcatcat ggtcttcgtc ctcgttgtca gctacttcct ggtcagcgcc cacaaccgcg
     2821 cccgggagga cctcatcgag acgcttacca tggaacgtga attgaggcag acccataaga
     2881 agctggcaac ggagatggcg ggtataacac aatcgagact tctggcgctc aaggccaagg
     2941 agcgcctggg gctcacaaag ccaaaagagg aagaggttct cgtgttgaaa tgaacaaaca
     3001 ggcgatcagg aaagccttct tcgtgtgcgc attcatcggg ctcgcatacg ccgcgctggc
     3061 tttcaatgtt caggacaggt tttcgatgaa aaggacctac gagacatcgg cgaagaaagc
     3121 tccccggagc ctgaacgtcg ccgtccccgg gccacagaaa gaagcgctcc ggcaggcgcc
     3181 ggtgatcgcc ggggacgacc tttctttcga accgtcgctg ctcatcgcaa ttcccatcgt
     3241 cgcctggtcg atcagggagg ggatcgtgga gaaggacggc atggtcctcg tgaagatgac
     3301 ggaggatcct tcttcggtct atctcaagaa acccctcgac atcctgaaag acagggacaa
     3361 ggagggtttg agggccctgg cgcggataat cggcagaaaa aacatggaga ccttcctgac
     3421 cagggaagga gtgaagctgc ccgaccggtc cctgtccttc gacaccctcg cgggcaccgg
     3481 atacagcgtg cacggcagga cgctcacggc gctctacaac aagtatgtcg atgccggctt
     3541 cgaccccctc atgccctacg ccagcggggg ccttgaggtc tcgcggaagg ccggcgggtt
     3601 cacgatagga aaggtccgcg aggaaagaaa ggcgcaggcc gacccggaca gcgccgcatg
     3661 gacgatgccg gacctcggtg gtctttcgat gaaggccgcc ctcgacagga tatcgacgaa
     3721 aggacaggcg gtacgcatct acgggtccgg cgtcgtcacc gaccagtacc ccaaaccgcg
     3781 ggagcgggtc gagaaggaca cccggtgcat tctctacggg aggacatacc agaagtgaga
     3841 ttgagcgagc tcatacgggg catgaagaag accgccgtga ccggtgacac gtccctcgag
     3901 ataacgggcg tcacgaaaga ttcccggaac gtctccgagg ggtctctctt tttcgctacc
     3961 tccacgagca gcgcatacct caatgacgct ctggaacggg gggcgcgcgc cgtggtcacc
     4021 gacggagaca tcgaccgtac cttcgcgtgc gtcatccgcg tcgaggatgt gaagcgggca
     4081 ctcggcgagg ccgcgtcgaa gttccacggc cacccctccc gcaagatgca tgtcacgggc
     4141 atcacgggga ccaacggcaa gaccaccacg acctatctga tcgagtccat attcgaggcg
     4201 gcgggaaaaa gacccggcgt catcggcacc atatcctacc gctacgccgg caagacactc
     4261 aatgcgccca acacaactcc cggcgccagc gagacgaacg cccttcttcg cgacatgatg
     4321 gacgccggga taagccacgt gatcatggag gtctcctctc acgcgctcca tcagtcacgc
     4381 gttgaaggta tcgacttcga tgtcgccgta ttcaccaacc tcacccacga ccatctcgat
     4441 tatcacggcg acttcgaaag ctacaaggcc tcaaaaaggc ttctcttcga agattttctc
     4501 gcaaggagca ggaagaagag aagatatgcc ataatgaata tcgacgatcc ctccatacgg
     4561 gacttcgccg tcgccccacc ggtgacgatg ctgtcctaca gcacgcgggg tttcgctgat
     4621 gcctgcctgt cgagctattc cgagagcatc gacggcctga cactcacctt atccctaccg
     4681 gggaagaaga tccccattgt ctcaccctta gtcggcatgt tcaatgcctc caacatcctt
     4741 gccgcatcat tgtcaggcca tgtcacaggt atatcgcccc aggcgataaa ggaagggatc
     4801 gaacggctgg ggggcgtccc gggaagactg gagcgagtgg cgaacgacag aggcatttcc
     4861 atctttgtcg attacgccca caccccggac gcgctcgaaa atgtcctcag tctgctcgga
     4921 aggctcaaga aagggaggct catcgtggtc ttcggttgcg ggggcaacag ggatgccgtg
     4981 aagagacccc tcatgggcgc catcgccgcg cgcttcgccg acgaggtgat cgtcacctcc
     5041 gacaacccgc gcaacgagga cccgctcacg atcatcgagg acatcagacg gggcctcgaa
     5101 ggcaaggcgg cgaggatcat cccggacagg cgcgaggcca tctttgaagg catcgggtcg
     5161 gcgagacccg acgacgtcgt gcttgtcgca ggcaagggac atgaggatta ccagatcatc
     5221 ggcggaacca cccttcactt cagcgaccgc gaagtaatcg aggagtctct ccatgtggcg
     5281 acttgaggat atcgtgaagg cggtgggagg aacggtcctc aaaaaggaaa aggaggtgtt
     5341 cgcggccatc tcgacggatt cccggacgat cgggaaaggg gacctcttca tccccattct
     5401 gggcaagtcc tttgacggtc acgcgttcat cggggaggcc ctcaaaaggt cgggaggggg
     5461 aacgctgtgc cgcgccgaca gccccgtgtc ccttgcggat gccgacggga ccgtcatcct
     5521 agtggaggac accaatcagg cgctcctcga cctggcccgc ttcaagaggc acaccctttc
     5581 gggcgccttc atctcgatca cgggaagcaa cggcaagacg acgacgaagg agatcctcgc
     5641 cgccatgatg acgaaacgct acggcgtcca cttcaacgaa aagaacctga acaacctcat
     5701 cggtgtgccg atgagcatcc tctccgttgc cggggacccc gagatatgca tcctggagct
     5761 gggcacgaac acgcccggcg agataaaaaa gctcgccgtg gcaacgaacc ccgatgcatc
     5821 cctcatcacg aacgtgaacc cctcccatct tgaagggctc gcgtcgctcg agggcatcct
     5881 cgaggaaaag ctcgatctct tccggcacac gaaggaaggc gggaagatat tcgtcaacat
     5941 cgacgatccc ggcatagccg gctggtggcg aacaacacag aggacaaccg tcaccttcgg
     6001 catcgacagc gacgccgact tccgtctcgc agtcggggaa gacctcggat gggaaggctc
     6061 cgatgtcatc atcagctgcc cggcggggac catacgggcg cggacttctc tcctcggcag
     6121 gcacaacctc tacaacatcc ttgccgccgc cgcgatagcg tcgaccatgg gggtcgaagg
     6181 cgagctcatc ggggaggcca tcgggaagtt cgtttcctac gacaagagat tccagcccgc
     6241 gacaagccca aagggctacg tcatcatcga cgatacgtac aacgcgaacc cctcgtctat
     6301 gaagtgggcc ataagcaccc ttgcagctct tcccgcggca gggaaaaggg tggccgtcct
     6361 cgggggcatg aaggaactcg gcgaaatgag cgcgcggtac cacagggagc taggggcata
     6421 cttgcgggaa gcagggctcg ggctcatcgt ccttctcggt gaagagacga aggacaccat
     6481 ggcggagctc ggtggagcgc cggcggcgca tttcgacaac aaggaagaac tgatcggcta
     6541 cgtggcgtcg caggcgggac cgggcgacac catcctggtg aaagggtccc gggcattcag
     6601 gatggaagat atcgtggagg cgctgaagta atgctgtatt atctcctcta ccagctgcac
     6661 atgaaggtct ccgggttcaa cgtgttccgg tatataacct tcaggaccgt cctgtccata
     6721 ctcacggcgc tcatcatcag cttcatcctc acgccctacg tcatcaggaa gttccatgac
     6781 tggaagatcc ggagcggcaa gcgcgaagac gtaccggacc gacacgaagc aaagaaggaa
     6841 acgccgacga tgggagggtt cgtcatcctc atcgccacca tgatcccgac gcttctctgg
     6901 gcggacctca ccaactatta tatatgggtc gtcagcttct cattcgtgtc cttcggggcc
     6961 atcggcttca tggacgacgt gaagaagctc aaaggggaaa aggggaaagg catctcggga
     7021 cggatgaagc tctccctgca gatattcttc ggcctctgca tcggcgcgct catcttcttc
     7081 aagtacgggt tcgtcacgca gctcaccatt cccttcttca agaacctccg gcccgatctg
     7141 ggcgtcttct acatcgccct gtgcatattc atcatcgccg ggacatccaa tgccgtgaac
     7201 cttaccgacg gcctcgacgg cctcgccatc ggccccgttc tcaccatctg ctccacattc
     7261 atgctcttct cctacctcgt gggcaacgtt atcttcgcgc agtacctcca gatattctac
     7321 gtgaagggtg ccggggagct cacggtcctg tgcggggcaa tgctcggcgc cggcatcggg
     7381 ttcctgtggt acaacgcgca cccggcggag ctcttcatgg gggacacggg ctcactctcc
     7441 ctgggcgcgg ccctcgcaac gatagccgtc atcatcaagc aggaatttct cctcgtcatc
     7501 gccggcggga tcttcgtcct ggagacgctg tccgtcatca tccaggtata ctccttcaaa
     7561 ctgaggggca agagggtctt tcgcatggcg cccatacacc accatttcga gctgaagggg
     7621 tggaacgagg aaaagatagt ggtgcgcttc tggatcatct cggtgatcct gggattgatt
     7681 gcgttaagta cgctgaaatt gaggtaagat ggatatacct gaccgcatat tgatcgtcgg
     7741 actcggtgcc tcggggacag ccgcggcgcg gttcctttcg cgaaagggga agacgatcgg
     7801 acttgccgac gaaaagaacg cggccgaact ggccggaccc ctcacggacc tcgaaggcat
     7861 acccttcacc ggccatttcg gtccccatcg cgccgaggat ttcgcctcct atcccctcat
     7921 cgtcctcagc cccggcgtcg acagcgaact tccggtgctg aggcgcgcgc gcgaagcagg
     7981 agcgcgcgtc atcggagaga tggagctcgc cgcgtccttc atcgaggaac ccatcatagc
     8041 catcaccggc accaacggaa agacgacgac gaccaccctt atcggtgagg tcttctcgcg
     8101 ggcattcggc ggcgtcttcg taggaggcaa cataggcaat ccgcttatca actacgtcgc
     8161 cgagggcaag aagacctccc acgtcattgt cgaggtgagc agcttccaac tcgagacgat
     8221 agagaccttc aggccggcga cggcggtgct cctcaacatc accgaggacc acctggaccg
     8281 ttaccgcagc tacgacgagt acaggcaggc aaagtaccgc atcttcgaca accaggggag
     8341 cggggattct gccgttatca gaaagggtct cgatgtcgtc atgaaggggt caccgcgcgt
     8401 gctgtccttc tcggtgacgg aggaactcga tgagggcgcc
//
LOCUS       DAOPOA010000011         8433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_9404_length_8433_cov_3.497255, whole genome shotgun sequence.
ACCESSION   DAOPOA010000011 DAOPOA010000000
VERSION     DAOPOA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9404_length_8433_cov_3.497255"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1934)
                     /locus_tag="PKJ17_00445"
     CDS             complement(<1..1934)
                     /locus_tag="PKJ17_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOD74460.1"
                     /translation="MSHKKFSVINTPVHNIDGIAKVTGRATYTFDVKLPGMLYGKILR
                     SPHPHAKIINIDTTRAMELPGVHAVVTGKDTLGIKQGIWRRYEELCDEQILPLEKVRY
                     IGEPVAAVAAVTEEIAEAALDLIDVEYEVLPAVYEPLEAIKKDAPTIHEGVERNINVT
                     RHIEWGDVDEGFEQADYIREDWFKCSGQHHMCMETRAAVSNFTPEGKLTVWTSTQSAY
                     YHQALLAGVFGLREGDVRILAPYVGGGFGGKFEIDSAQFCSGILSMKLHKPVKIVLTR
                     EEDFIASKRRTPMFYYVRTGMKKDGTFVARESRMFSNGGAYTGMGATALYLSGFFHSF
                     PYKWQSYRYDGYRVYTNTMPSTSMRGFGAPQAMFCSEQQIDWMCADLGLDPFEVRRKN
                     GHTEGYEVPGQAFIASCGLEQCINEIEKWVKSKGKLPANRGIGISSCGFMSGGIFNWF
                     DSPYSFSSAVITINVDGTVELHVGCQDIGQGSNTTMAIIAAETLGVKVEDIRVHSGDT
                     AHCPADLGAWGSRQTLMAGNAVKMACEDAKKQLLEFAYAEMGINIVYDLDIKDRWVHI
                     VARPERGMKYEDLVKKALRAKDGQRIMGRGYYTPHRKGMISPAYSYMMQAVDAEIDPE
                     TGKIKIHEVMTAHDCGQPINP"
     gene            complement(1936..2421)
                     /locus_tag="PKJ17_00450"
     CDS             complement(1936..2421)
                     /locus_tag="PKJ17_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOD74461.1"
                     /translation="MKKQVLTFRVNGDEYEILIHPKTLLADAIREHVGLTGTKRGCDT
                     VSCGACTVMLNGMSVKSCSVLAIQCEGMDITTVEGLEENGKLSPIQRAFLDNGSYQCG
                     FCTPGMLMSSTAFLNEMGDKKPSEEEIRESIEGNICRCTGYNSIVRAIDAVAQGKYKE
                     E"
     gene            complement(2433..3305)
                     /locus_tag="PKJ17_00455"
     CDS             complement(2433..3305)
                     /locus_tag="PKJ17_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOD74462.1"
                     /translation="MINDFQYLKPASLKEALAMYAEHDDCKVICGGQSLLIVLRQGML
                     APEYLVDIKHVNELKYIKFDEKDGLRIGATTTHREVEKSDIIAKKYPALVAMEQKLAS
                     IQTRNWGTIGGNLAHADPAGDLAPVLIALNAEIKFGNVDGEKTVPLDEFFVDYFETQM
                     GHGDLVLEAHVPAPEPKSGCAYQKFNLLDSEMGIVAAAASVVLNADGTCKNARIVLGN
                     AGPTPLRVTKAEDLLKGKKLDDKLFTEAGQIASEEAQPVADIHASEEHRRHVLGVLTK
                     RMLKKAFEQAQKAA"
     gene            complement(3375..3605)
                     /locus_tag="PKJ17_00460"
     CDS             complement(3375..3605)
                     /locus_tag="PKJ17_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74463.1"
                     /translation="MECAIYRGRHYYLCYSPAYRGTSFDPERIKELKKEESADESDKT
                     FGMPGNIEVGSQCIRNVEEYGIEREFAALKKN"
     gene            3917..4939
                     /locus_tag="PKJ17_00465"
     CDS             3917..4939
                     /locus_tag="PKJ17_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOD74464.1"
                     /translation="MKRFFGVKCLLFVFFAATCLAFFPVMASAQKVITLNYSNFFPAP
                     HKNSVVAQQWCKEVEKRSGGKVKITYFPGGTLTPAAQTYDNVVKGIADIGESCFAYTR
                     GKFPMMEVIDLPLGYKSGVSATNLINAYYAKFNPKELADVKVLWLHAHGPGILLSKKP
                     VKTMDDMKGMKIRATGLAAKVVQALGGAPVGTTMPETYDALRTGVADGAMAPVEAAQG
                     WKWGEVVKYITQNFGSAYTTGMFVVMNKAKWNAMPPDVQKVFEEVSKEWVVKQGKVWD
                     EIDKEGYDFAKKRGNQVITLSKEEDAKWAKAVKPLLDDHVKTLKGKGLPGAEAVKFAV
                     DYLKKN"
     gene            complement(5109..5717)
                     /gene="hisH"
                     /locus_tag="PKJ17_00470"
     CDS             complement(5109..5717)
                     /gene="hisH"
                     /locus_tag="PKJ17_00470"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOD74465.1"
                     /translation="MIVVVDYGMGNLKSVLNAFAKLGAGVTVSGDPASIEGASAIVVP
                     GVGAFGRCMENLEGRGLAPAIREHIRRDKRYLGICLGMQVLFESSEEAPGVKGLGVIK
                     GTVPRFTGSMKVPHMGWNSVDIRKESPILKGINPGDFFYFVHSYYCAPDDGSVIATTT
                     EYDGPFTSAVETGSLFACQFHPEKSQRVGLALLRNFLALAGC"
     gene            complement(5718..6302)
                     /gene="hisB"
                     /locus_tag="PKJ17_00475"
     CDS             complement(5718..6302)
                     /gene="hisB"
                     /locus_tag="PKJ17_00475"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOD74466.1"
                     /translation="MARKAEVERTTKETSISVSWDLDGSGEYSISTGIPFFDHMLDLF
                     AKHGLFDLVVTAKGDTDVDNHHTVEDVGIAMGKALRDALGGMEGIRRYGSALVPMDET
                     LCMTAVDISGRSSFVFKGRLQGRTGGFDVDVVKEFLQAFVNEARLTLHVNLLYGSNLH
                     HKVEAVFKSFARTLRAAVEKDDRIKGVLSTKGVL"
     gene            complement(6312..7181)
                     /gene="hisG"
                     /locus_tag="PKJ17_00480"
     CDS             complement(6312..7181)
                     /gene="hisG"
                     /locus_tag="PKJ17_00480"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOD74467.1"
                     /translation="MKLKIGLPKGSLQESTFRLFKNAGYTIKLRERSYVPVIDDDEME
                     GLVIRAQEMARYVEDGILDMGITGADWVLEQDARVVELVRLRYGKVGFRGVKWVVAVP
                     NNSPIKKVKDLQGKKIATELVAFTKRYLKKQGIEASVEFSWGATEVKPPLLADAIVEV
                     TETGASLVANNLRIVETILESETVLIANRTAWKDPWKKRKMENLIILLKGALLAEEKV
                     GLKMNIPRNKLGRVTEILPSLHTPTVSNLSDDKWVAIEVIIDEKVVRDIIPDLKRAGA
                     QGIVEYPLKKVIM"
     gene            complement(7178..>8433)
                     /gene="hisD"
                     /locus_tag="PKJ17_00485"
     CDS             complement(7178..>8433)
                     /gene="hisD"
                     /locus_tag="PKJ17_00485"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOD74468.1"
                     /translation="FDGLMSFVKTGREGKKRDVREAVLKIRQAVLSRGDAALVDFSIA
                     WDGWKAAHPLKLTSREIGEAASRVPARDISVLKGMIRNVTSYHKGQKMPKRVFRKKGL
                     VVEEGPVPVESAVVYVPGGTASYPSSLVMGVVPAQIAGVKRVCATTPGRGGAINDHVA
                     AAAKLLGIDEVYRVGGAQAIYAFAYGTGSVPKVDMIVGPGNAYVEEAKRDVYGSVGID
                     MLAGPTELVVLCTEAHSPKMVALDMFSQAEHDELASVGLFSSSREHLSDVMKSIEGLI
                     DGAPRRETIRKALDDNGYMVHYTDLGRAVEAINSIAPEHMELLGDTTCLPSILYPGVI
                     YVGLNTPVAMGDYYIGTNHVLPTAGAGRFTGGLSVDRFTKRKVVVTIDRTFIEKYGDR
                     AERLARIEGLPAHGDAIKARKESPK"
BASE COUNT         1603 a         2481 c         2408 g         1941 t
ORIGIN      
        1 gggttgatgg gctggccgca gtcgtgtgcg gtcatgacct cgtggatctt gatctttccc
       61 gtttcggggt cgatctcggc gtcgacagcc tgcatcatgt agctgtatgc gggggatatc
      121 atgccttttc tgtgaggcgt gtagtagccg cggcccatga tgcgctggcc gtctttcgcg
      181 cggagcgcct tcttgacgag gtcttcgtat ttcatgcctc tttccggacg ggcgacgata
      241 tgcacccacc tgtccttgat gtcgaggtca tagacgatgt tgatgcccat ttccgcgtag
      301 gcaaattcca ggagctgctt cttcgcgtcc tcacaggcca tcttcacggc gttgcctgcc
      361 atgagggtct ggcgtgagcc ccatgcgccg aggtcggcgg ggcagtgcgc ggtgtcgccg
      421 gagtggaccc tgatgtcttc cactttcacg cccagggtct ctgccgcgat gatcgccatc
      481 gtggtgttcg agccctggcc gatatcctgg cagcccacat ggagctcgac ggtaccgtca
      541 acgttgatgg tgatgacagc cgaggagaat gaatagggtg agtcgaacca gttgaagatg
      601 ccgcccgaca tgaaaccgca ggaggatatg ccaatgcctc tgttcgcggg aagcttgcct
      661 ttcgatttga cccatttctc gatctcgttg atgcactgct cgaggccgca gctggcgatg
      721 aatgcctggc ccgggacttc gtagccttcc gtgtggccgt tctttcttct cacctcgaag
      781 gggtcgaggc cgaggtccgc gcacatccag tcgatctgct gctcggagca gaacatggcc
      841 tggggggcac cgaaaccgcg catcgacgtc gagggcatgg tgttcgtgta aacacggtag
      901 ccgtcgtagc ggtaactctg ccacttatac ggaaaggagt gaaagaagcc cgacaggtag
      961 agggccgttg cacccatgcc cgtgtaggcg ccgccgttac tgaacatgcg ggactcgcgg
     1021 gcaacgaagg tgccgtcctt cttcatgcct gtcctgacgt agtagaacat gggggtgcgc
     1081 ctcttggagg caatgaaatc ctcttcacgg gtgagaacga tcttcaccgg cttatggagt
     1141 ttcatggaga ggatgccgct gcagaactgg gcggagtcta tctcgaactt accgccgaag
     1201 cctcctccga catagggtgc gagaatgcgg acatctccct cgcgaagccc aaagacgcct
     1261 gccaggaggg cctggtggta gtacgcggac tgcgtggatg tccagaccgt gagcttgcct
     1321 tcaggggtga aatttgaaac ggcggcccgg gtctccatgc acatatggtg ctggccggaa
     1381 cacttgaacc agtcttccct gatgtagtcg gcctgctcaa acccctcgtc gacatctccc
     1441 cactcgatgt gacgggtgac gttgatgttt ctttcgacgc cctcgtggat cgtgggagcg
     1501 tctttcttga tggcttccag cggctcgtaa acagcgggaa gaacttcgta ttccacatcg
     1561 atgaggtcga gggccgcttc cgcgatctcc tccgtcacgg ccgcgacggc cgcgacgggt
     1621 tcgccgatat aacggacttt ttccaaggga agtatctgtt cgtcgcaaag ctcttcgtag
     1681 cgtctccaga tgccctgctt tatgcccagc gtgtccttgc cggtcacgac cgcatggacc
     1741 ccggggagtt ccatcgccct ggtggtgtct atgttgatga tcttcgcgtg aggatggggg
     1801 ctgcggagga tcttcccgta gagcatgccg gggagcttca cgtcgaatgt gtatgtggcc
     1861 cggcccgtta ccttcgcaat gccatctata ttgtggaccg gcgtattgat gactgagaat
     1921 tttttgtgac tcatattact cctccttata tttgccctgg gcaacggcgt caatggccct
     1981 tacgatactg ttgtagcctg tgcaccggca gatattgcct tcgatgcttt cccggatctc
     2041 ttcctcggaa ggtttcttgt cccccatctc attgaggaac gccgttgagg acataagcat
     2101 gcccggggtg cagaacccgc actggtagga gccgttgtcg aggaacgccc tctggatggg
     2161 gctcagcttg ccgttctcct ccaggccttc gaccgtcgtg atatccatcc cttcgcactg
     2221 gatggcgagg acggagcagg atttcacgga catgccgttc agcatgaccg tacaggcgcc
     2281 gcaggaaacc gtgtcgcagc ctctcttggt gccggtgaga cctacgtgct cacggatggc
     2341 gtccgccagc aaggtcttcg gatgtataag gatttcgtat tcgtcaccgt taactctaaa
     2401 agtcaatacc tgttttttca tatctctcct ccttaggcag ccttctgggc ttgttcaaag
     2461 gccttcttca gcatcctctt ggtgagaaca ccaagcacat gccttctatg ctcttcggat
     2521 gcgtgaatat cggcgacagg ctgtgcttcc tccgaagcga tctggccggc ctcggtgaag
     2581 agcttgtcgt caagcttctt gccctttaaa aggtcttcgg ccttcgtcac tctaaggggt
     2641 gtgggacctg cgttgccgag aacgatccgg gcgtttttgc acgtgccgtc ggcgttgagc
     2701 acaaccgagg cggcagcggc aacgataccc atctcgctgt cgaggagatt gaatttctga
     2761 taagcacaac cggatttcgg ctccggtgcg ggaacgtgcg cctcaagaac gagatcgcca
     2821 tgacccatct gcgtctcgaa gtaatcgacg aagaactcgt cgaggggaac ggtcttttcg
     2881 ccatcaacgt tcccgaactt tatctccgca ttgagggcga taagaacggg tgcgaggtcg
     2941 cctgcgggat cggcatgggc gaggttcccg cctatggttc cccagttgcg cgtctggatc
     3001 gacgcgagct tctgctccat cgcaacgagg gcaggatatt tcttcgcgat gatatcggat
     3061 ttctcgacct ccctatgggt cgtcgtcgcg ccgatcctaa gaccgtcctt ctcatcgaac
     3121 ttgatgtact tgagctcatt gacgtgcttg atatccacga gatattcggg ggcgagcatg
     3181 ccctgcctca agacgatcaa caaggactgg ccgccgcaga tgactttgca gtcatcgtgt
     3241 tccgcgtaca ttgcaagcgc ttctttcagg cttgcaggct tcaggtactg aaaatcgtta
     3301 atcataggtc tcctctcctt tctattgttt ctacttcttc aacgccgagc cgagccaacg
     3361 cttgccggca gagatcagtt cttcttcaag gcggcgaatt cgcgctcgat gccgtattcc
     3421 tcgacattcc tgatgcactg cgacccaacc tctatgttgc ccggcatccc gaaggtcttg
     3481 tccgactcgt cggcggactc ctctttcttc aactccttga tcctctccgg atcgaaggac
     3541 gtaccccgat aggccgggga atagcacagg taatagtgtc ggccgcggta gatagcgcat
     3601 tccacgcaag cctttccgga tatgggacat aatattttgt gtttggccat aggcagcacc
     3661 cctgaataaa gattggtaat gcctgaaaag cattatgtgg caaaattatt gcggatattt
     3721 gttcaaaagt caagtttatt tttttcccat aatgaacata tgtctgtatt gcataaaatt
     3781 gcaaggtttt attgcacagc atttgttcat catgatgaac tccgtgattc ttcgcccctg
     3841 ttttgtgtgc aattgtgttg acaaatggta ccccgtttac tataaagaga gaaaatcaca
     3901 tttcaggagg ggttttatga aacggttttt cggggtaaag tgtttgcttt tcgttttctt
     3961 cgctgcaacc tgtcttgcct tctttccagt aatggcgtcg gcccagaagg tcatcacgct
     4021 caactactcc aatttcttcc cggcgccgca taagaacagc gtcgtcgcgc agcagtggtg
     4081 caaggaagtt gagaagaggt cgggcggcaa ggtcaagata acctacttcc ccggtggcac
     4141 cctcaccccc gccgcgcaga cctatgacaa cgtcgtgaaa ggcatcgcgg acatcggcga
     4201 gagctgcttc gcctacacca ggggcaaatt ccccatgatg gaagtcatcg accttcccct
     4261 cggctacaag agcggcgttt ccgcgacgaa cctcatcaac gcctactacg ccaagttcaa
     4321 cccgaaagag cttgccgacg tcaaggtgct ctggcttcac gcacacggcc ccggcatcct
     4381 tctctccaag aaacccgtca agacgatgga tgacatgaag ggcatgaaga tccgcgcgac
     4441 cggtctcgcg gcgaaggtcg ttcaggcgct cggcggcgca cccgtcggca caacgatgcc
     4501 tgagacctat gacgcgctcc gcaccggcgt ggccgatggc gccatggctc ccgttgaggc
     4561 ggcacagggc tggaaatggg gtgaggttgt caagtacata acccagaact tcggctccgc
     4621 ctataccacc ggtatgttcg tggtcatgaa caaggcaaag tggaacgcca tgcctcccga
     4681 cgtccagaag gtcttcgaag aggtcagcaa ggaatgggta gtgaaacagg gtaaggtctg
     4741 ggatgagatc gacaaggaag gctacgattt cgccaagaag agaggcaatc aggtcatcac
     4801 tctctccaag gaagaagacg cgaaatgggc aaaggccgtc aagccccttc ttgatgacca
     4861 cgtaaaaacc ctcaagggta agggtcttcc cggcgccgag gcagtcaagt ttgcggttga
     4921 ttatctgaag aagaactaaa cgaacaagat atagctaaaa aagggctccg aaaccggagc
     4981 cctttttttg tcgcaggtcg ccagagattg ccgcgagggg ggtgggccgt cacatttttg
     5041 tggagcggcc aaccgattct tcttttggct tctaacttgt gacctgaaac ctgggacttg
     5101 tgacgattct aacacccggc cagggcaagg aagttccgga ggagcgcaag gcccacgcgc
     5161 tggctttttt cggggtggaa ctgacaggcg aagagggagc ccgtctcgac ggctgaggtg
     5221 aaagggccgt cgtactccgt ggtcgtggcg attacggatc cgtcgtcggg ggcgcagtaa
     5281 taggaatgca cgaagtagaa gaaatcgccc gggtttattc ccttgaggat ggggctctcc
     5341 ttccggatgt caacgctgtt ccatcccatg tggggcactt tcatggagcc cgtaaagcgc
     5401 ggcacggtgc ccttgatgac cccgagaccc ttgactcccg gcgcctcctc gcttgattcg
     5461 aagagcacct gcatcccgag gcatatgccg aggtaccgct tgtccctgcg gatgtgctct
     5521 ctgatggcgg gggccaggcc cctgccctcc aggttctcca tgcacctgcc gaaggcgccc
     5581 acccccggca cgactatggc ggaggccccc tcaatggacg ccgggtcgcc ggacacagtg
     5641 acgccggccc cgagcttcgc gaatgcgttg aggacgcttt tcaggttgcc catcccgtaa
     5701 tcgacaacaa cgatcattta cagtacgcct ttcgtgctga gtacgccttt tatgcggtcg
     5761 tccttctcca ccgcggccct caaggtccgg gcgaaggatt tgaacacggc ctccaccttg
     5821 tggtggaggt tgcttccgta gaggaggttg acgtgaaggg tcagcctcgc ctcgttcacg
     5881 aaggcctgga ggaattcctt caccacgtcg acgtcaaatc ctcccgttct tccctggaga
     5941 cgccctttga agacgaagga ggaccgtccc gatatgtcga cggccgtcat gcagagcgtc
     6001 tcatccatgg gaacgagcgc gctgccgtaa cgccttatcc cttccatgcc gccgagggcg
     6061 tccctcaggg ccttccccat ggcgatgccc acatcctcca cggtatggtg gttgtccaca
     6121 tcggtgtccc cttttgccgt cacgacgagg tcaaagaggc cgtgcttcgc gaagagatcg
     6181 agcatgtggt cgaaaaaggg tatccctgtc gagatgctgt actcgcccga gccgtcgagg
     6241 tcccaggata cggagatgga cgtctctttg gtcgtccttt ccacttcggc cttcctcgcc
     6301 atatctcccc cttacatgat gacctttttc aggggatatt ccacgatccc ctgcgccccg
     6361 gcccttttca ggtcggggat tatatctctg acaacctttt cgtcgatgat gacctcgatg
     6421 gccacccact tgtcgtcgga aaggttcgat accgtcggcg tgtgcagaga gggaaggatc
     6481 tccgtcactc tgccgagctt gttcctgggg atgttcatct tgagcccgac cttctcttcc
     6541 gcaaggagcg cccccttcag gaggatgatg agattttcca tcttcctctt cttccagggg
     6601 tccttccaag cggtcctgtt cgcgatgagg acggtctcgg attccaagat ggtctccact
     6661 atcctcaggt tgttggcgac cagcgacgct cccgtctcgg tcacctccac gatggcgtcg
     6721 gccagaaggg gcggcttaac ctccgtggcg ccccaggaga actccacaga ggcctcgatc
     6781 ccctgttttt tgagataccg cttcgtgaag gccacgagct ccgttgcgat cttcttgccc
     6841 tggaggtcct tcaccttctt gatgggagaa ttgttgggca cggcgacgac ccacttgacg
     6901 ccgcggaagc ccaccttgcc gtagcgaaga cggacgagct ccaccaccct ggcatcctgc
     6961 tccaggaccc agtcggcccc cgttatcccc atgtcgagga tcccgtcctc cacgtagcgg
     7021 gccatttcct gggcccgtat gacgaggcct tccatctcgt cgtcgtctat gacggggacg
     7081 taggagcgct cccggagctt gatggtgtag ccggcgttct tgaaaagccg gaaagtcgat
     7141 tcctgcaggc tgcccttggg cagaccgatc ttcagtttca tttgggggac tcctttcgtg
     7201 cctttattgc atcgccatgg gcgggcaggc cctctatcct tgcgagcctt tccgccctgt
     7261 cgccatattt ttcaatgaat gtccggtcta ttgtaacaac aaccttccgc tttgtaaacc
     7321 ggtcgacgga aagcccccct gtgaaccgtc ccgcgcccgc cgtgggcaga acgtggttcg
     7381 tcccgatata gtagtcgccc atggcaacag gcgtgttcag accgacgtag atgacgccgg
     7441 gatagagtat cgagggcaga cacgttgtgt cgcccaggag ctccatgtgc tccggcgcga
     7501 tgctgtttat cgcctcgacg gcgcggccga ggtcggtgta gtgcaccatg tacccgttgt
     7561 cgtcgagggc cttcctgatc gtctccctgc gcggggcacc gtcgataagc ccttcgatgc
     7621 tcttcatgac gtcgctgagg tgttcccggg aagaggagaa gagccccacc gatgccagtt
     7681 cgtcgtgttc ggcctgtgag aacatgtcga gggccaccat cttaggcgag tgggcctcgg
     7741 tgcagagaac gacaagctcc gtcggcccgg cgagcatgtc gatgccgacg gacccgtaaa
     7801 cgtccctctt ggcctcttcc acgtaggcgt ttcccggccc gacgatcatg tccaccttcg
     7861 gcaccgagcc cgtcccgtac gcgaaggcat atatggcctg ggcgcctcct acgcggtaga
     7921 cctcgtcgat gccgaggagt ttcgcggcgg cggccacgtg gtcatttatg gcaccccccc
     7981 ggccgggcgt ggtcgcgcag accctcttca ccccggctat ctgcgccggt accaccccca
     8041 tgaccagcga cgacgggtag gacgccgttc ccccggggac gtagaccacg gcgctttcca
     8101 ccggcaccgg gccttcctcg acgacgaggc ccttcttcct gaagacccgt ttcggcatct
     8161 tctgtccctt gtggtaggaa gtcacgttcc tgatcatccc cttgaggacg gaaatgtccc
     8221 ttgccgggac cctcgatgcc gcctcaccga tctcacggga ggtcagtttc agggggtgcg
     8281 ccgccttcca gccgtcccag gcgatggaga agtccacgag agcggcgtct ccgcgggaaa
     8341 ggacggcctg gcgtatcttc aggaccgcct cgcggacatc gcgcttcttg ccttccctgc
     8401 cggtcttcac gaaggacatg aggccgtcga att
//
LOCUS       DAOPOA010000012         8293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_9628_length_8293_cov_3.987861, whole genome shotgun sequence.
ACCESSION   DAOPOA010000012 DAOPOA010000000
VERSION     DAOPOA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9628_length_8293_cov_3.987861"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PKJ17_00490"
     CDS             <1..871
                     /locus_tag="PKJ17_00490"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOD74469.1"
                     /translation="GKPTVINNVETLSQVPLVLAAGPEGYRKLGTKESPGTKTFALTG
                     HVANSGLIEMPFGATLREIIYGVAGGMTSDTGKAVSDGFKAVQIGGPSGGCLTRDHLD
                     LPIDFDSLKTVGAMVGSGGLVVMNKNTCMVSVARFFMQFTQHESCGKCVLCREGTKQM
                     LALLDDIIEGRATMETLDLLEDLAAAVQKGSLCGLGKTAPNPVLSTMKHFRDEYIAHV
                     RDKRCPAGKCKALVRIVIDPELCKGCTLCATKCPVGAISGEKGKPHVIDQNMCRKCYI
                     CVEACKFQAIKGD"
     gene            875..2899
                     /locus_tag="PKJ17_00495"
     CDS             875..2899
                     /locus_tag="PKJ17_00495"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOD74470.1"
                     /translation="MNGTVVYIDNKEIPIENEQNLLELVRKARIDLPTFCYHSELSIY
                     GACRLCMVQVEGMGLVPACSTPPAPEMKVSTNTEEIRKMRRIIVELLLANHSQNCPTC
                     QKSTTCQLQALAKRLGITKVRFKNQLKEMPLDESSPSLIRDPNKCVLCGDCVRICSEV
                     QTVGAIDFAYRGAQTVVIPSFGKALDQVECVNCGQCARICPTGALTPRSEVDEVWQAI
                     HDPSKIVVAQVAPAVRVAIGEMFGLPPGTTTTGQIAAALRAIGFDKIYDTSFTADLTV
                     LEESNEFIKRVTAGDRIPQFTSCCPAWVKFVEQYYPEFVNHLSSCRSPQQMFGALAKE
                     MLSSELDVARKDIVVVSIMPCTAKKFEAKRPEFESDGVRDVDNVLTTQELGRMIEETG
                     LHFRELGPESFHLPFGFKTGAGVIFGNSGGVSEAALRYVSEKLTGQKLDTYEFASVRG
                     EEGIREAKLSIAGKEFSFAVVSGLGNARRILEKIRTGEAHYDLVEVMACPGGCIGGAG
                     QPVFTDPAVKRKRTKGLYENDRMLELHKSQDNPYITELYEKVLGDVGGHKAHKLLHTG
                     YRTRKRIFDEDVDISSGGEKSIDVSVCFGTSCFLKGSQKLLRDILEHIRVKGLGARVN
                     VKASFCFEKCDKGPTIKVGSRIIDGCTIEKAIEAIKREGVDLAEEVRQNA"
     gene            2892..4628
                     /locus_tag="PKJ17_00500"
     CDS             2892..4628
                     /locus_tag="PKJ17_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOD74471.1"
                     /translation="MLETPKPQIVFTLTARCRDCYRCLRACPVKAIRMQNGQAYVDEK
                     RCISCGTCIRECPQEAKTFRSDINIAQHLVDEGHFVAASIAPSFPAVFNKWQRSRLAS
                     ALRALGIRYVGQTSHGAYQISLESQRLAKADTTKPHITTACPALINYIEKYKPELIDY
                     LLPVASPMVAHARLLKEKLGKDVKVIFIGPCVAKKSELNRPDVAGVVDCVLTFRELNA
                     WLDQKNIDLSMCEESNFDEKPVHNAQLYPLPGGLIKTAGLDDDGLNLSLLRVDSPQGV
                     RELLNGIGEGSEHALIEPLFCSQGCINGPGIDSGKNLFERRQEIIEYTTESAAVSIIP
                     DPVESDLLKAAFNRNDSGPAPDSVTEQQIEEILARTGKQDPEQQLNCGACGYDSCREK
                     AIAVALGMAEPEMCIPYMRRLAERRTDQIFSTTPNGVLMLDDELNILGMNPAFKKFFS
                     CSDVILGRHISYLMDPAPFEKLISGVTETLDITVLHRPYNLLCRELLYMLKEEKQIVG
                     IFINITSQQEHEKKLREIRSQTVEQANELLEHQIKMAQNIAQFLGESTARGEELVRKL
                     MSLSEGDETNPR"
     gene            4609..5778
                     /locus_tag="PKJ17_00505"
     CDS             4609..5778
                     /locus_tag="PKJ17_00505"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD74472.1"
                     /translation="MKPTPDDPRKYIEAANAQAAKKPGGICGDYIVEERTAEATTVIV
                     ADGIGTGIKARVAAVMCASRLMELIRVGFTLREACHKVVDTMHAARTMDIPFSAFSVC
                     RVLVSGHATVVSYEIPSPILINNRLAAYLPKQRVFPMGLEMVGEVNCMLEYGDGIIMV
                     SDGVSQAGLGQQYRMGWGVQGACDYINGLLAQGMRLKDIPGRIVANVRDISGATYGDD
                     TTCSLLLCREARAINILTGPPARKMDDARVVREFMDSKGMRIVCGSSTAEIVARNLGV
                     PVEMKNMSNAYHKPPSYDIKGVDYATEGAITLNQVYNILGEAPEKLEPDSSVSDLYRF
                     FHASDTINFLVGTAANPGHEHIVFRQMGVYPRDVIVRLLAEKLKSMGKLVNIIYL"
     gene            5834..7414
                     /locus_tag="PKJ17_00510"
     CDS             5834..7414
                     /locus_tag="PKJ17_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HOD74473.1"
                     /translation="MTAGAPLDFFNAADYFVDRNVRQGRGHKTAVYTEYRNYTYNDIQ
                     KMANKTANAFREVGITVDDRILVIMLDVPQFYAVFWGAMKIGAVPIPVNTMLTPEDYE
                     YYLNDSRAKVLVVSEQLLPILAQIKGDLLYLRDLVVISETKGAHIPFKQKYRHAPNTV
                     KTEFTTKDDVGFWLYSSGSTGSPKGAIHSQYDMVVTSESFAKGILGITEDDIVFSAAR
                     LFFSYGLGNGMYFPMSVGASAVLSAHAPSPENVFRHLEKFRPTIFFGVPTLYGQMLEY
                     KAKEDKERGIKKANPNDQHELSSVRVCVSAGEALPVDIFERWKKRFGIEIIDGIGSTE
                     MLHIFLSNRPGDIKPGSTGKPVPGYELRLVDESGQEVPRGEIGTLHVKGDSAAQQYWR
                     KREKTRVTMQGEWVNTGDKYYIDEDGYYWCAGRADDMLKVGGIWVSPIEVENTLSQHP
                     AVLECAVVGAPDAKNLIKPKAFVILRNGYEKTEELKEELKKWVLDRQAKFKYPRWVEF
                     VDELPKSATGKIQRFRLR"
     gene            7717..>8293
                     /locus_tag="PKJ17_00515"
     CDS             7717..>8293
                     /locus_tag="PKJ17_00515"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD74474.1"
                     /translation="MTRAFFTVAIVFMFFLSFHSFAVAGPTKPAASKQQTCASCHSEF
                     SSLLSAKHPRVTGKDIKACAPCHAPDPRNTAKPKAYSAKLHLGHMQAGNELDCLVCHT
                     WQPGKAFGVKGAKKPIGVLTKENMDQTKKVFASWASSKYLDAHHGKKNVTCAGCHEKL
                     PESGDSVENDRCLTCHGDMEQLARKTEPKDFP"
BASE COUNT         1931 a         2547 c         2393 g         1422 t
ORIGIN      
        1 gggtaaaccg acggtcatca acaacgtcga gaccctgtcg caggtgcccc tggtactggc
       61 cgccggcccc gagggatacc gcaagttggg cacgaaggaa tccccgggca cgaagacctt
      121 tgccctaacc ggccacgtgg cgaactcagg cctcatagaa atgcctttcg gcgcgaccct
      181 gcgggagatc atctacggtg tcgccggagg catgacctcc gatacgggca aggcggttag
      241 cgacggtttc aaagccgtgc agataggagg tccctccggc ggctgcctta cccgcgacca
      301 cctggacctt cccatagact tcgattccct gaagaccgtc ggcgccatgg tcggctccgg
      361 cggccttgtc gtcatgaaca agaacacctg catggtcagc gtggcgcgtt tcttcatgca
      421 gttcacgcag cacgagtcct gcggcaagtg cgtcctctgc cgcgaaggga cgaagcagat
      481 gcttgccctc ctggacgata tcatcgaggg acgggcaacg atggaaacgc tcgaccttct
      541 cgaggacctc gcggcagccg ttcagaaagg ctcgctctgc gggctcggca agacggcccc
      601 caatcctgtc ctttcaacaa tgaaacattt ccgcgacgag tacatcgcgc acgtgcggga
      661 caagcgctgc cccgcgggaa aatgcaaggc cctggtaaga atcgtcatag accccgaact
      721 gtgcaagggc tgcacgctct gcgcgacgaa atgtcccgtc ggcgccatat cgggagaaaa
      781 agggaagccc catgtcatcg accagaacat gtgcagaaag tgttacatct gcgtggaggc
      841 ctgcaaattc caggcgatca aaggagactg agcaatgaac ggcaccgttg tctatataga
      901 taataaagag atacccatcg agaacgaaca gaacctcctg gagcttgtca ggaaggcgag
      961 gatagacctg cccaccttct gctatcattc cgagttgagc atatacggcg cctgcaggct
     1021 ctgcatggtc caggtcgagg gcatgggcct cgttcccgcc tgttccaccc cgcccgcccc
     1081 cgaaatgaag gtgagcacca acacggagga gatccggaag atgcgcagga tcatcgtgga
     1141 gcttctcctt gcaaaccaca gccagaactg tcccacctgt cagaagagca ccacatgcca
     1201 gcttcaggct ctcgcgaaac gcctcggcat caccaaggtg cgcttcaaga accagctgaa
     1261 ggaaatgccc ctcgacgaat cctcgccgtc cctcatcagg gatcccaaca agtgcgtcct
     1321 atgcggtgac tgcgtgcgca tctgctcgga ggtgcagacc gtcggcgcca tcgacttcgc
     1381 ataccgggga gcacagacgg tcgtcatacc ctccttcgga aaggccctcg accaggttga
     1441 gtgcgtgaac tgcggccagt gcgcccgcat ctgccccacc ggcgcgctga ccccccggtc
     1501 tgaggtggac gaggtctggc aggccatcca cgacccttcc aagatcgtcg tcgcccaggt
     1561 ggcgcccgcc gtgcgggtgg ccatcggcga gatgttcgga ctgccccccg gcaccacgac
     1621 gacgggacag atcgcggcgg ctttgagggc catcggtttc gacaagatat atgacacgtc
     1681 cttcaccgcg gacctcacgg tgctggaaga gagcaacgag ttcataaaaa gagtgaccgc
     1741 aggcgacagg atcccgcagt tcacgtcatg ctgccccgca tgggtcaagt tcgtcgagca
     1801 atactacccc gagttcgtca accatctgtc gagctgccgc tccccccagc agatgttcgg
     1861 cgcgctggcg aaagagatgc tttccagcga gctcgacgtc gcccgcaagg acatcgtcgt
     1921 cgtctccatc atgccctgca cagccaagaa gttcgaagcc aagaggccgg aattcgaaag
     1981 tgacggcgtg agggatgtgg acaatgtcct gaccacccag gaactgggga ggatgatcga
     2041 ggagaccggc cttcatttca gggaactcgg cccggaatcc ttccacctgc cctttggctt
     2101 caagaccggc gccggcgtca tattcggcaa ttccggcggc gtcagcgagg ccgcgctgcg
     2161 ctacgtcagc gagaaactga cgggccagaa actcgatacc tacgagttcg cgtccgtccg
     2221 gggcgaggag gggatccgcg aggcaaaact ctccatcgcc ggcaaggaat tctccttcgc
     2281 cgtcgtgagc ggtctcggta acgcgagacg gatccttgag aagatacgca ccggcgaggc
     2341 gcattacgac ctcgtcgagg tcatggcatg ccccggcggc tgcatcggcg gagcgggcca
     2401 gcccgtgttc accgaccccg cggtcaagcg gaaaaggaca aaggggctct acgagaacga
     2461 ccgcatgctg gagctccaca aatcgcagga caacccctac atcacggaac tctacgaaaa
     2521 ggtgctcggc gacgtgggag gccacaaggc acacaagctt ctccacacgg gctaccgcac
     2581 ccgcaagcgc atattcgacg aggacgtgga cataagctcc ggcggagaga agagcatcga
     2641 cgtcagcgtc tgcttcggta cgagctgttt cctcaagggc tcccagaagc tgctgcgcga
     2701 catcctcgaa cacatacgcg tgaaggggct gggcgcgagg gtcaacgtga aggcatcctt
     2761 ctgctttgag aaatgcgaca aaggaccgac aattaaagtg gggagcagga tcattgacgg
     2821 ctgcaccata gagaaggcca tcgaggccat aaagcgcgag ggggtcgacc tcgccgaaga
     2881 ggtaaggcag aatgcttgaa acaccaaaac cgcagatcgt cttcacgctc acagcccgct
     2941 gccgggactg ttaccggtgc ctcagggcct gtcccgtcaa ggcgatccgc atgcagaacg
     3001 gccaggcgta cgtggatgag aagcgctgca tctcctgcgg cacctgcatc cgcgaatgcc
     3061 cgcaggaggc aaagacattc cgcagcgaca tcaatatcgc ccagcacctt gttgacgaag
     3121 gacatttcgt agccgccagc atagccccct catttcctgc cgtattcaac aagtggcaac
     3181 gctcgcggct cgcctcggcg ctccgcgccc tcggcatcag gtacgtggga cagacatccc
     3241 acggggcgta tcagatatcg ctcgaatccc agagactcgc gaaagccgat acgacaaagc
     3301 cccacataac cacggcatgt cccgccctga taaactacat cgaaaaatac aaaccggagc
     3361 tgatcgatta cctgcttccc gtcgcgtccc ccatggtggc gcacgcaagg cttctcaaag
     3421 agaagctcgg caaggacgtt aaggtcatct tcatcggccc ctgcgtggcc aagaaatcgg
     3481 aattgaaccg gccggacgtt gccggcgtcg tggactgtgt cctcaccttc agggaactca
     3541 acgcctggct tgaccaaaag aacattgacc tgtccatgtg cgaggaaagc aacttcgacg
     3601 agaaacccgt tcacaatgcg caactgtacc ccctgcccgg cggtctcatc aagacggccg
     3661 gcctcgatga tgacggattg aacctcagtc ttctgcgcgt cgacagcccc cagggcgttc
     3721 gcgaacttct gaacggtatc ggagaaggat cggagcacgc cctgatcgag cccctgttct
     3781 gcagccaggg gtgcatcaac ggtcccggca tagactccgg caagaacctc ttcgaacgca
     3841 ggcaggagat catcgagtac acgacggaaa gcgcggccgt ctcgatcatt cctgaccccg
     3901 tcgaaagcga tctcctgaag gccgcattca acaggaacga cagcggcccc gccccggaca
     3961 gcgttacgga acagcagata gaggagatcc tggcgcggac cgggaaacag gaccccgaac
     4021 agcagctcaa ctgcggagcc tgcggctacg acagctgcag ggaaaaggcc atcgccgtgg
     4081 ctcttggaat ggcggagccg gagatgtgca tcccctacat gaggcgtctc gcggaaaggc
     4141 gcacggatca gatcttcagc accacgccga acggcgtgct catgctcgac gacgaattga
     4201 acatcctcgg tatgaacccc gcgttcaaga aattcttctc ctgcagcgac gtcatcctgg
     4261 gacggcacat ctcctatctc atggatcccg cccccttcga aaagctcata tccggcgtca
     4321 cggagaccct ggacatcacc gtactccacc gtccctacaa cctcctgtgc cgcgagcttc
     4381 tgtacatgct caaggaggag aagcagatcg tcggcatctt catcaacata acgagtcaac
     4441 aggaacatga aaagaagctc agggagatcc gttcccagac ggtggaacag gcaaacgagc
     4501 tccttgaaca ccagatcaag atggcccaga acatcgcgca gttcctgggc gaaagcacgg
     4561 cccgcggtga ggaactcgtg cgaaaactga tgagtctgag cgaaggcgat gaaaccaacc
     4621 cccgatgatc caaggaaata tatcgaggcg gcaaacgccc aggcggcgaa aaaacccggc
     4681 gggatatgcg gcgactacat agtggaggaa cgcaccgccg aggcaaccac cgtgatcgtg
     4741 gccgacggga tcggtacggg catcaaggcg cgggttgccg ccgtcatgtg cgcctcccgc
     4801 ctcatggagc tcatccgcgt cggtttcacc cttcgcgagg cgtgccataa ggtcgtcgac
     4861 acgatgcacg cggcgcggac catggacatc cccttctccg ccttttccgt ctgccgcgtt
     4921 ctcgtgagcg gccacgcgac cgtcgtttcc tacgagatcc cctcccccat cctcatcaac
     4981 aaccgccttg cggcgtatct gccgaagcag cgcgtctttc ccatgggcct cgaaatggtg
     5041 ggcgaggtca actgcatgct cgaatacggc gacggcatca tcatggtaag cgacggggtc
     5101 agccaggccg gcctgggtca gcaataccgc atgggttggg gcgtccaggg ggcgtgcgac
     5161 tacatcaacg gtctcctcgc acagggcatg cggctgaagg acatccccgg caggatcgtc
     5221 gccaacgtga gggacatctc gggggcgacg tacggggacg acaccacgtg cagtctcctg
     5281 ctctgccgcg aggcacgggc catcaacatc ctgacggggc ctccggcccg gaagatggac
     5341 gatgccagag tggtcaggga attcatggac tcgaagggaa tgaggatcgt gtgcgggtcg
     5401 tccaccgccg agatcgtcgc ccgcaatctc ggcgtccctg tcgagatgaa gaacatgtca
     5461 aatgcctacc ataaaccacc gtcttatgat ataaaaggcg tggactatgc gacggagggt
     5521 gcgatcaccc tcaaccaggt ctacaacatc ctgggggagg cacccgagaa actcgaacct
     5581 gactcatcag tttcagatct ctacaggttt ttccacgcct ccgatacgat caacttcctc
     5641 gttggaacgg cggcaaatcc gggacacgaa catatagtct tcagacagat gggtgtatac
     5701 ccgagagacg ttatcgtcag gctcctcgcg gagaaactga aatccatggg gaaactggtg
     5761 aatatcatat atctataggg ggaacgtgaa acgggcaagc gcagccctcc cccgcacaac
     5821 agaaggaggt atcatgacag ccggagcacc acttgacttt ttcaacgcag ccgattattt
     5881 cgtggaccgc aacgtccgcc aggggcgggg ccacaagaca gccgtgtaca cggaataccg
     5941 gaactacacc tacaacgata tccagaagat ggccaacaaa acagcgaacg ccttccgcga
     6001 ggtcggcata accgtagacg accgcatcct agtgatcatg ctcgacgtac cgcagttcta
     6061 cgccgtcttc tggggcgcca tgaagatcgg cgccgtcccc attcccgtca acaccatgct
     6121 gacacccgag gactacgaat actacctcaa cgacagccgg gcgaaggtcc tcgtcgtctc
     6181 cgagcagctc ctgcccatcc tcgcgcagat aaagggcgac ctcctgtacc tgcgcgacct
     6241 cgtcgtcata tccgagacga aaggggccca catccccttc aagcagaagt accgccacgc
     6301 tcccaacacg gtgaagacgg agttcaccac gaaggacgac gtcggcttct ggctctacag
     6361 ctcgggctcg acgggctcac ccaagggtgc catccactct cagtacgaca tggtggtcac
     6421 ctccgagtcc ttcgccaagg gcattctcgg catcaccgag gacgacatag tcttctcggc
     6481 ggcgcgactc ttcttctcat atggtctcgg caacggcatg tatttcccca tgtccgtcgg
     6541 cgcctcggcc gtgctcagcg cccacgcccc gtcgccggaa aacgttttca ggcatctcga
     6601 gaagttccgc cccaccatct tcttcggcgt ccccacgctc tacgggcaga tgctggagta
     6661 caaagccaaa gaggacaagg aaaggggcat caagaaggcg aaccccaacg accagcacga
     6721 actctcgtcg gtcagggtct gcgtgtccgc cggcgaggcg ctgcccgtgg atatcttcga
     6781 gcgctggaag aaacgcttcg gcatcgagat catcgacggc atcggctcca cggagatgct
     6841 ccacatattc ctgtccaaca ggcccgggga catcaagccc ggatccacgg gaaaacccgt
     6901 tcccggttac gagctgagac tcgtcgacga gagcggccag gaagtccccc ggggcgagat
     6961 aggcaccctg cacgtcaagg gcgacagcgc cgcccagcag tactggcgca agcgcgagaa
     7021 aacgcgggtc accatgcagg gagagtgggt caacacgggc gacaagtact acatcgatga
     7081 ggacggttat tattggtgcg ccggacgtgc cgacgacatg ctcaaggtgg gcggcatctg
     7141 ggtctcaccc atcgaagtgg agaacacact gagccagcac ccggccgttc tggagtgcgc
     7201 cgtcgtcggc gcccccgacg cgaagaacct catcaagccc aaggccttcg tcatcctgag
     7261 gaacgggtac gagaaaaccg aggagttgaa agaggaattg aagaaatggg tactcgacag
     7321 gcaggcgaag ttcaaatacc cccgctgggt cgaattcgtc gacgagcttc cgaagagcgc
     7381 gacgggtaag atacagaggt tcagactgag gtaaagaagg ttcaagagtt ccagggttca
     7441 agagttcaag ggccttggga caaagatacg aaagaccctc cctgtccgac ggatggggag
     7501 ggttgctttg ccccgggaat gtcgggaatt tgttcttgaa taacaggtat gaccggggtt
     7561 atagatatac aaagcggata tttgaggtgt gacggaagcg atcgatgccg gaattgtcat
     7621 cggggctttg cagcggcaga gggtcgacac gggagacatg gttcatcgag agcggggttt
     7681 gccctgttcc ttagatcaaa gagagagggt tgagaaatga ccagagcgtt tttcactgtg
     7741 gcgatcgttt tcatgttttt cctgtcgttc cattccttcg cggttgctgg acccacgaag
     7801 ccagccgctt ccaaacagca gacctgcgcg agttgccata gcgaattctc ctcgctcctg
     7861 tccgcgaaac acccgcgtgt cacagggaaa gatatcaagg catgcgctcc ctgccacgcc
     7921 cccgaccccc ggaacacagc gaagccgaag gcctattccg cgaagctcca tcttggtcac
     7981 atgcaggcgg gcaacgagct cgactgcctc gtgtgccata cctggcagcc ggggaaggcc
     8041 tttggcgtaa agggagccaa gaaacccatc ggtgttctca cgaaggaaaa tatggaccag
     8101 acaaagaagg tctttgcctc ctgggcctca tcaaaatatc tcgacgccca tcacggcaag
     8161 aagaacgtga cctgcgccgg ctgccacgag aaactgcccg agtcgggcga ctccgtcgag
     8221 aacgaccggt gccttacctg ccatggcgac atggaacaac tcgccaggaa aacggaacca
     8281 aaggattttc ctg
//
LOCUS       DAOPOA010000013         8280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_9651_length_8280_cov_3.382249, whole genome shotgun sequence.
ACCESSION   DAOPOA010000013 DAOPOA010000000
VERSION     DAOPOA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9651_length_8280_cov_3.382249"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="PKJ17_00520"
     CDS             <1..1422
                     /locus_tag="PKJ17_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74475.1"
                     /translation="VYRTYINGPDVKERDRHYIELALSRAIRKNPALNESIFLFMKRV
                     LLLEFSADMDEEMRGTWLNFAMKFQQTTGPVMAKGVEDTAFYVYNRLVSLNEVGGSPD
                     RFGTSPDTFHGQNIERIKNWPYALITSSTHDTKRSEDVRARINVLSEIPGEWREHVVL
                     WAKVNRKRKMLVNNRKVPDSNEEYLLYQTLVGSWPFGTVSDDEYESYKGRIKAYMAKA
                     AKEAKVNTSWINPDRMYEEAMTLFVDSILDRRRDNPFLADFVPFQQRVSLFGMYNSAA
                     QTLLKICSPGVPDFYQGTEVWNLSLVDPDNRMPVDHGLRMRMLDEMKEQLSRRDTAAY
                     AKDLTSSMEDGRIKLYITYKALGLRRKMREVFEKGEYIPLEAAGTRSEHVIAFARRFL
                     NTMIIVVVPRFVAKVLSSPGMTFEDMWEDTMVIVPESSEGATFRNVLTGEELARTRRQ
                     GLSGLACSGLFRNLPVALLETGR"
     gene            1446..2678
                     /locus_tag="PKJ17_00525"
     CDS             1446..2678
                     /locus_tag="PKJ17_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD74476.1"
                     /translation="MAQVLAMILAGGRVGELDVLTFYRPKSVLPYGGLYRVIDFPMSN
                     LMHSDIENVGILSQYRPFELISHIANGEPWDMTGRHRRAVILPPFQGRDASDWYKGTA
                     DAVYQNIDFIRMNDPGIVVVLSGDHIYRMDYRKLIEFHLEKNADLTVAFTKVVPEASS
                     RFGLAFIEDEDPRGGRVTQYREKPRNSKLEWASLTIYVFRTELLVAALEENAAHSSHE
                     FGKDIIPMLMENHRVYGYKHSGYWGYTRTIGEYYRTSMDVLGDKPRLDIRSWQLRTNM
                     ADRSIRDRQPAFVGPSASVEDAFFHCGCVIKGRVARSILFPGVTVEEGAVVEDSILFF
                     DSAVRGGAHVKRTIADTGCVISERADVGGAGDDLAVIGMGTVIPEGVRIRGGATVHPK
                     LKADAFSKKEYLSGEVVQ"
     gene            2675..3931
                     /locus_tag="PKJ17_00530"
     CDS             2675..3931
                     /locus_tag="PKJ17_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD74477.1"
                     /translation="MKGPAAILLAGGVGSRLNILVRHRAKPAVPFGGIYRIIDFTLSN
                     IGNSEMTSVAVLTQYKPLSLMDHIGDGSSWDLSGRSRSVKIFPPKTGEEDWDWYKGTA
                     DAVRQNLSFVVSAESDEIMILSGDHVYHMDYRRMVSFHREKKARVTIAAIPVPWEETY
                     QFGTAVTDDSNRIVSWEEKSPRARSNLASMGIYVFDRKYLIDLLEESKEDDFGHHIIP
                     RALEEKVVFAYPFDGYWRDVGTVQAYWDANMDLLDPSSGIDPGSWRTMTNISAREPAF
                     DRPPVLVSSGARVVRSILSPGCTVEGHVENCVLSPGVAVERGAFVRDSIIMHDCRIGE
                     GARVERCIFDKEVVVGAHTTVGIGDPKTVNRTHPEHLYSGLTIIGKQSIIPGDITIGT
                     NCIINPMLGSRSFTGEVGSGTTITGA"
     gene            3947..5209
                     /locus_tag="PKJ17_00535"
     CDS             3947..5209
                     /locus_tag="PKJ17_00535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD74478.1"
                     /translation="MADTAIKREDIQARVTRIGKADILVGIPSYNNARSIGHVVRAVQ
                     AGLAKYFPDRKTVLVNSDGGSTDGTMDVVHDTTIDDFRSILLSHRVESFSKMTIPYHG
                     IPGKGSAFRTIFEIAQALDVKACAVVDSDLRSITPEWIELLVKPVMEDGYDYVSPLYH
                     RHKYDGTITNSIVYPLTRALYGRNVRQPIGGDFGFSGKLAGFYLSKDVWETDVARYGI
                     DIWMTTTAIANDFRVCQAFLGAKIHDAKDPGADLSAMLYQVVGATFGLMEEYPRTWKS
                     VTRTQPVPVFGFTYEVGLEPVSVNIQRMIDKFTLGARELVPVWENVLSEETLGFLKEL
                     ADAPQDNFHMPDAVWVDIIYSFALAAHRNLLNKEHLLKSLTPLYIGRTASFVIEAWES
                     SGPEVEEKIENLCGVYEKLKPSFSDRWK"
     gene            5228..5935
                     /locus_tag="PKJ17_00540"
     CDS             5228..5935
                     /locus_tag="PKJ17_00540"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74479.1"
                     /translation="MNFLQRVIIEPFESSYEKFLTFMPNFLTSLALLIVGIVLAAVLR
                     MVFLRIFRAINLDKAAQRLGLFDMLLKGGIRDSLSAFIARIIGWLVIFTFAVVAVQTL
                     KIPTVEHLLERFFLYLPNVFTAAVILLFGYLFSNFFGRAALIASVNAGIQRAGLVGRL
                     VKITVFILSATMAMEQLGIGRDTVLIAFAIIFGGIVLALAIAFGFGGRDIARTYLQRT
                     VGKEKENEENRDDINPL"
     gene            6072..7106
                     /locus_tag="PKJ17_00545"
     CDS             6072..7106
                     /locus_tag="PKJ17_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74480.1"
                     /translation="MIEFRMVESESTFLDPRKGFEPTTVRFQVRVDPLTGRTCHFSHF
                     GAVKAQRLDLAGYHDPAVKGFCPFCAEHRERTTPRFTPDVVPEGRLENGEALLIPNLF
                     PYDIHSGVVVMTDDHVVGLKDFTRKRLDDAFSLGIDFLRRIADLDGSLPYHLMTWNYM
                     PPSGGGLVHPHQQYFATACPGNQFMDELSASRRFAERHRQNYWYALARQEATGNERYI
                     ARIGSGHWIASFVSLGLLGEIMCIFPDVFSLQDLTDDDIDDLVSGLERVFRYFEEAGV
                     FSFNASLCLGPRDQEHFSCYFRIVPRTFLNTRDFAPDLNFFQAILSEPICVVLPEDLC
                     REVKPYFNQG"
     gene            7361..7933
                     /locus_tag="PKJ17_00550"
     CDS             7361..7933
                     /locus_tag="PKJ17_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOD74481.1"
                     /translation="MDIIELVTKNRSHRRFQESEPITMEQLEGLVELARLSPSAANLQ
                     PLKFVLSNDPARNASIFSCLAWEDYIRNSRGPAEGRRPSGYIVVLGDTTITKDFSCNH
                     GIAAQSILLGATEMGLGGYMFAGVDKARLATLLRIPREYEILLVIALGVPRERVVVTE
                     ARDGNIAYWRDINKVHHVPKRPLAEIILDL"
     gene            8012..>8280
                     /locus_tag="PKJ17_00555"
     CDS             8012..>8280
                     /locus_tag="PKJ17_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOD74482.1"
                     /translation="MLELVTKSRSYRRFHEDRTISLQQLKGLVELARLSPSARNLQPL
                     KFILSHDSRKNAAIFSCLAWAGYLKDWPGPEEGERPSAYIIILGDG"
BASE COUNT         1797 a         2458 c         2350 g         1675 t
ORIGIN      
        1 gtctacagaa cctacataaa cggccccgac gtgaaggaaa gggaccgtca ttacatcgaa
       61 ctcgccctgt cccgggccat caggaaaaat cccgccctca acgagtcgat ctttctcttc
      121 atgaaacgtg tcctgctcct ggaattctcc gccgatatgg atgaagagat gcgcgggacg
      181 tggctcaatt tcgcgatgaa gttccagcag acgaccggcc ctgtcatggc aaagggtgtg
      241 gaggacacag ccttctatgt gtacaaccgg ctcgtgtccc tcaacgaggt cgggggcagc
      301 ccggacaggt tcggcacctc gccggacacc tttcacgggc agaatatcga gcgcatcaag
      361 aactggccct acgccctcat cacctcctcc acccatgaca cgaagcgtag cgaggatgtg
      421 cgcgcaagga taaacgtgct ttccgagatc cccggggaat ggagggagca tgtcgtcctg
      481 tgggcaaagg tgaaccggaa acgaaagatg ctcgtcaaca acaggaaggt gcctgacagt
      541 aacgaggagt accttctcta ccagacgctc gtcggcagtt ggcctttcgg cacggtgagc
      601 gacgatgagt acgagtcata caagggacgg atcaaggcgt acatggccaa ggcggcaaag
      661 gaagcaaagg tgaacaccag ctggataaac cctgacagga tgtatgagga ggcgatgacg
      721 ctctttgtgg attccatcct cgacagaagg agggacaacc cctttctcgc tgacttcgtc
      781 ccctttcagc agcgcgtgtc cctcttcggc atgtacaatt ccgcggcaca gacgcttctc
      841 aagatatgtt ctcccggcgt acccgacttc taccagggca cggaggtctg gaacctcagc
      901 ctcgtcgacc ccgacaacag gatgcccgtc gaccacgggc ttcgcatgcg catgctcgac
      961 gagatgaagg aacagctctc ccggcgcgac accgccgcat acgcgaaaga tttgacaagt
     1021 tccatggaag acggcagaat aaagctgtac ataacgtata aggcgctcgg tcttcgccgg
     1081 aagatgagag aggtctttga gaagggtgaa tacattccgc tggaagcggc aggaacccgg
     1141 tcggaacacg tcatagcctt cgcgcgtcgt ttcctcaata cgatgatcat agtggtcgtt
     1201 ccgaggttcg tggcgaaggt gctgtcttcg ccgggcatga ccttcgagga catgtgggag
     1261 gatacgatgg tgatcgttcc cgaaagctcg gaaggagcga ccttcagaaa cgtcctcacc
     1321 ggcgaagaac tggcgcgaac ccgccggcag gggttatcgg gacttgcgtg ttccgggctt
     1381 ttcaggaatc ttcctgtcgc gcttctcgaa accggtcgtt gatcgaccgg gaaaggagtc
     1441 gtctcatggc acaggtgctc gcaatgatcc tcgcgggagg cagggtgggc gaactcgacg
     1501 tgctcacctt ctaccggccc aaatccgtcc tgccttacgg cgggctgtat cgcgtcatcg
     1561 atttcccgat gagcaacctc atgcattccg acatcgagaa cgtgggtatc ctgtcgcagt
     1621 acaggccctt cgaattgatc agccacatag cgaacggcga accctgggac atgacgggaa
     1681 ggcaccgcag ggcggtgata ctcccgccct ttcagggccg ggacgcctcc gactggtaca
     1741 agggaacggc cgacgccgtg taccagaaca tcgacttcat acggatgaac gaccccggga
     1801 tcgtcgtggt cctttccgga gatcacatct accgaatgga ctatcgcaag ctcatagagt
     1861 tccacctgga gaagaacgcg gacctgacgg tcgccttcac gaaggtcgtc cccgaagcct
     1921 cgtcccgttt cggccttgcc ttcatagagg acgaggaccc ccggggagga agggtcactc
     1981 aatacaggga gaaaccgaga aactcgaaac tggaatgggc gtcccttacc atttacgtct
     2041 tccggacgga actgcttgtc gccgccctcg aggagaatgc cgcacactcc tcccatgagt
     2101 tcggtaagga cataatcccc atgctcatgg aaaaccaccg cgtttacggc tacaagcaca
     2161 gcggttactg ggggtacacc aggacgatag gcgaatacta ccggacgagc atggatgtcc
     2221 tgggggacaa acccaggctg gacatacgct cctggcagct caggaccaac atggcggata
     2281 gaagcatcag ggaccggcaa ccggccttcg tcggtccttc ggccagcgtc gaggacgcct
     2341 tcttccattg cggttgcgtc atcaagggcc gcgtggcccg ctccattctc tttccaggcg
     2401 tgaccgtcga ggagggagcg gtcgtcgagg attccattct cttcttcgat tcagccgtcc
     2461 gcgggggggc gcatgtcaag cgcaccatag cggataccgg ctgcgtaata tccgaacggg
     2521 ccgatgtcgg aggcgcgggc gacgaccttg ccgtcatcgg tatgggaacg gtcattcccg
     2581 aaggggtccg catacggggc ggtgccacgg tccatcccaa gctcaaggcc gacgcgttct
     2641 cgaagaagga gtatctcagc ggggaggtgg tccaatgaaa ggtccggccg cgatccttct
     2701 ggcaggcgga gtgggaagca ggctcaacat cctcgtccgg cacagggcga aacctgccgt
     2761 ccctttcggt ggcatataca ggataatcga cttcaccctc agcaacatcg gcaattcgga
     2821 aatgaccagt gtggccgttc tgacgcagta caagcctctt tccctcatgg accacatagg
     2881 cgacggctcc tcctgggacc tgtcgggccg ctcgcgcagc gtcaagatct tcccccccaa
     2941 gacgggagag gaggactggg attggtacaa aggcacagcc gacgccgtgc gccagaacct
     3001 ttccttcgtc gtctcggccg aaagcgatga gatcatgatc ctgtcgggcg accacgtcta
     3061 tcacatggac taccgccgga tggtctcctt tcaccgggag aagaaagccc gcgtcaccat
     3121 cgccgccatt cccgtgccct gggaggaaac ataccagttc gggactgccg tcacagacga
     3181 cagcaacagg atagtaagct gggaggaaaa atcgccaagg gcaaggtcca acctcgcctc
     3241 gatggggata tatgtcttcg acaggaaata tctgatcgat ctcctcgagg aatccaagga
     3301 agatgatttc gggcaccaca tcatcccccg ggcccttgag gaaaaggtcg tcttcgctta
     3361 tcctttcgac ggatactggc gcgacgtggg caccgttcag gcgtactggg acgcgaatat
     3421 ggaccttctt gacccatcct cggggataga cccgggatcc tggaggacaa tgacaaacat
     3481 aagcgcccgg gaaccggcct tcgacaggcc acccgtcctg gtatcttccg gcgcccgggt
     3541 ggtgcgttcc atcctgtcgc ccggctgcac cgtcgagggg catgtggaga actgcgtcct
     3601 gtcgcccgga gtcgcggtcg aacgcggcgc cttcgttcgc gactccatca taatgcatga
     3661 ttgccgcata ggtgaagggg cacgcgtcga acgctgcatt ttcgacaagg aggtggtcgt
     3721 gggtgcgcat accacggtcg gtatcgggga cccgaagacg gtcaaccgga cccacccgga
     3781 acatctctac tcgggcctca ccatcatcgg aaaacagtcg atcatccccg gagacatcac
     3841 cataggcaca aactgcatca tcaaccccat gctgggctcc agatccttca cgggagaggt
     3901 cgggagcgga acgaccataa ctggagcgtg aaagcgaaag gaacatatgg ctgacaccgc
     3961 gatcaagaga gaggacatcc aggcgcgggt gacccggatc ggcaaggccg acatacttgt
     4021 gggcatcccc agctacaaca acgcccgatc catcggccac gtggtacggg ccgtgcaggc
     4081 aggtctcgcc aagtatttcc ccgaccgcaa gacagtcctc gtgaattccg acggaggctc
     4141 gacggacggg acgatggatg ttgtccatga cacgacgatc gatgatttcc ggtcgatcct
     4201 cctttcccac cgtgtggaat ccttttccaa gatgaccatc ccctaccacg ggatccccgg
     4261 caagggaagc gctttcagga ccatcttcga gatcgcgcag gccctcgacg tgaaagcctg
     4321 tgccgtggtg gactccgacc tgcgcagcat cacccccgag tggatagaac ttctcgttaa
     4381 gcccgtcatg gaggacggct atgattacgt ctctcccctt taccatcgcc acaagtacga
     4441 cgggacgatc acgaacagca tcgtctatcc cctgacacgg gccctctacg gcaggaacgt
     4501 gcgccaaccc ataggcggtg acttcgggtt ctcggggaaa ctcgcgggat tctatctgtc
     4561 caaggacgtc tgggaaaccg acgttgcccg ctacggcata gacatctgga tgacgacaac
     4621 ggccatagcc aatgacttcc gggtctgcca ggcctttctc ggggccaaga tccacgatgc
     4681 caaggacccg ggggcggacc tttcggccat gctctaccag gtcgtggggg caaccttcgg
     4741 cctcatggag gaatacccga gaacatggaa gagcgtgaca cggacacagc cggtgcccgt
     4801 attcgggttc acctacgagg tcggcctcga accggtgagc gtcaacatcc agagaatgat
     4861 cgacaagttc acactgggcg ccagagaact tgtccccgtg tgggagaacg tcctgtccga
     4921 agagaccctc ggcttcctca aggaactggc cgacgcgccg caggacaatt tccacatgcc
     4981 tgacgcggtg tgggtggaca tcatatacag cttcgccctg gccgcgcatc gcaacctgct
     5041 caacaaggaa caccttctca agtcgctgac cccgctgtac ataggacgca cggcgtcatt
     5101 cgtgatcgag gcctgggaaa gcagcggacc cgaggtggaa gagaagatag agaacctctg
     5161 cggggtatac gagaagctca agccgtcctt ctcggaccgc tggaagtaaa acgaaagggg
     5221 gctttacatg aattttcttc agagggtcat tatagagcct ttcgagagct cttacgagaa
     5281 gttcctcaca ttcatgccca atttcctcac ctccctggcg cttctcattg tgggcatcgt
     5341 ccttgctgcg gtcctcagga tggtctttct aaggatcttc cgggccataa acctcgacaa
     5401 ggcggcccaa cgcctcgggc ttttcgacat gctcctcaaa ggcgggatca gggactccct
     5461 ttcggccttc atcgcaagga taatcggatg gctcgtcatc ttcaccttcg ccgtcgtggc
     5521 ggtgcagacg ctcaagatac ccaccgtcga gcacctcctg gagcgtttct tcctctatct
     5581 ccccaatgtc ttcacggcgg cggtgatcct tctcttcggc tatcttttca gcaacttttt
     5641 cggacgcgcc gcgctcatag cgtcggtcaa cgcgggaata caaagggccg gcctcgtcgg
     5701 caggctggtc aagatcaccg tcttcatcct gtccgccaca atggcgatgg aacagctcgg
     5761 gatcggccgt gataccgtgc tcatagcctt tgccattatc ttcggaggca ttgtcctggc
     5821 cctcgccatc gccttcggct tcggcggaag ggacatcgcc aggacctatc tgcaaaggac
     5881 cgtggggaaa gagaaggaga atgaagagaa ccgggacgac atcaatccgc tgtagagttt
     5941 taagttgtca ggttttaggt tttaagttga agaccgtcgt tgtcctgacc cttatccgtt
     6001 ctttccattg acaggcggtt ggggattctg ctcttatttt gagaaataca acgacaggga
     6061 gatgacggcg catgatcgag ttcaggatgg tggaatcgga aagtactttc cttgacccgc
     6121 gaaagggctt cgagcccact accgtacgtt tccaggtgcg tgtcgatccg ttaacgggca
     6181 ggacctgtca tttttctcat ttcggcgccg tcaaagccca gagactcgat ctggccgggt
     6241 atcacgatcc cgccgtaaag ggtttctgtc ccttctgcgc cgaacatcgc gaaaggacaa
     6301 cgccgagatt cacgcccgat gtcgttcccg aggggaggct ggagaacggg gaggcgcttc
     6361 ttatacccaa tctattcccc tatgacatcc acagcggtgt cgtcgtcatg accgacgatc
     6421 acgtcgtcgg cctgaaggac ttcacccgaa agaggctcga tgatgccttt tccctgggca
     6481 tcgacttcct gcgccgcata gcggatctcg acggttcgtt gccctatcac ctcatgacat
     6541 ggaactacat gcccccttcg gggggaggcc tcgttcatcc ccatcagcaa tacttcgcca
     6601 ccgcatgccc cggcaaccag ttcatggatg agctgtccgc atcacgacgc ttcgcggaac
     6661 gccacaggca gaattactgg tatgccctcg cgagacagga ggcgacaggc aacgagcggt
     6721 atatcgcacg gatcggttcg ggacactgga tcgcttcttt cgtctctctc gggctgctcg
     6781 gagagatcat gtgcatcttt cccgacgtct tttccctcca ggacctcacc gacgacgata
     6841 tcgacgacct cgtttccgga ctcgagcgcg tcttccggta tttcgaggaa gcgggggtct
     6901 tcagcttcaa cgcgtcgctc tgtctcggcc cccgcgacca ggagcacttc agctgctact
     6961 tccgcatcgt cccccggacg ttcctcaaca cgcgtgactt tgccccggac ctcaatttct
     7021 tccaggccat cctctccgaa cccatctgcg tggtcctccc ggaagacctc tgcagggagg
     7081 taaagcccta cttcaaccag ggatagttgc aggttcaagg gttcaaagac atttcaagag
     7141 ttcaagggct caagagtaaa agaagaaaac aacggacgct tttggcgtgt aagcacgatt
     7201 cgagggaagg accgcgaagg gggatcggtt gtcaatacta ttcgcgtacc ggccaaaggc
     7261 atccggcgcc ttttggtctt ctactcttga actcttgaac ttttgaaccc ttgaactttc
     7321 ttttgcttgc atgcccctcc ttctatccta tagttcctgt atggatatca tcgaactggt
     7381 cacgaagaac aggagccacc ggcggtttca ggagagcgaa cccataacca tggaacagct
     7441 ggagggcctc gtggagcttg cccgtctttc gccgtcggcc gcgaatctcc agcctctcaa
     7501 gttcgttctc tccaacgacc ccgcccgcaa cgcgtccatt ttctcctgtc ttgcgtggga
     7561 ggattatatc aggaactccc gcgggcctgc cgaaggccgt cggccgtcgg gatacatcgt
     7621 cgtcctcggt gataccacca tcacgaagga cttctcgtgc aaccacggga tcgccgcgca
     7681 gagcatactc ctcggcgcca cggagatggg tctgggcgga tacatgttcg ccggggtgga
     7741 taaggccagg cttgcgactc ttctccgtat accccgggaa tacgagatac tgctcgtcat
     7801 cgccctgggc gttcccaggg aacgtgtcgt cgtcacggag gccagggacg gcaacatcgc
     7861 ctattggcgc gacatcaaca aggtccacca cgttcctaaa cgccccctgg cggagatcat
     7921 actggatctt tgaaaaaatg ttccaggttc aagggttcca gagtttcagg tttttgaatc
     7981 ttacgtcaca gcgatgggag atggcatgac tgtgctggag cttgttacga agagcaggag
     8041 ttaccggagg tttcacgagg acaggacgat cagtttgcag cagctgaagg ggcttgtcga
     8101 gcttgcccgc ctttcgccct cggcgaggaa cctgcagccc ttgaagttca tcctgtccca
     8161 tgactcccgg aagaacgccg ccatattctc gtgccttgca tgggcaggct atctcaagga
     8221 ctggccgggc cccgaggaag gcgaaaggcc ttccgcctat atcattatcc tcggggacgg
//
LOCUS       DAOPOA010000014         8210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_9750_length_8210_cov_3.341263, whole genome shotgun sequence.
ACCESSION   DAOPOA010000014 DAOPOA010000000
VERSION     DAOPOA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9750_length_8210_cov_3.341263"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PKJ17_00560"
     CDS             complement(<1..723)
                     /locus_tag="PKJ17_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74483.1"
                     /translation="MPKRTDIKSVLIIGSGPIVIGQACEFDYSGSQACKAIREEGYRV
                     ILVNSNPATIMTDPDMADRVYVEPLIPEVMEEIIKTERPDVILPTLGGQTGLNLATIL
                     AERGILEKYGVELIGADYRAIKKAEGREEFKAAIEKIGLEVPRSGLAYTMEEARVVAR
                     EIGFPLIIRPSYTLGGTGASVAYNVEEFETLAVRGLESSLITEILIEESVIGWKEFEL
                     EVMRDRNDNVVIICSIENLDAMG"
     gene            complement(716..1831)
                     /gene="carA"
                     /locus_tag="PKJ17_00565"
     CDS             complement(716..1831)
                     /gene="carA"
                     /locus_tag="PKJ17_00565"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOD74484.1"
                     /translation="MKAFVALEDGTVFEGRGFGLEGEKEGEIVFNTSMTGYQEIMTDP
                     SYKGQIVTMTYPLIGNYGVNDEDVESGGPKVEGFIAREFSRITSNWRATGDLGDYFRK
                     HNIIAVDEIDTRALTRHLRVRGVMRGIISTTDHDRDSLVRKAQGSRGIVGVDLVSGVT
                     CQAPYEFEGGPGGDPAVDALHCVVMDFGVKRNILRILRQKGCRVTVVPAGTKAEDIVA
                     MRPDGVLLSNGPGDPEAVPYAIREIEKLIGRVPIFGICLGQQLLGLALGGRTYKLKFG
                     HRGANQPIRETATGKVYITSENHGFAVDVDSIRGQAVRVTHVNLNDGTAEGIEHETYP
                     AFSVQYHPEASPGPHDSYGLFEKFRNMMESSRKEGNA"
     gene            complement(1828..2514)
                     /locus_tag="PKJ17_00570"
     CDS             complement(1828..2514)
                     /locus_tag="PKJ17_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HOD74485.1"
                     /translation="MTGEEGRAGHNEKIEGWLRGLDVDTFGIADMSCYGEEITGIGDV
                     ATNELPFAVSFGIVLSVGVLSTLKDGPTQLYLHHYRQLNYRLDMIGYFLGRSIEREGF
                     RAIPFAASQVVDWQRQRGHINHKKVGVMAGLGWIGRNNLLVHPLFGSQVRYNTVLTDM
                     PLEAGRELGRDCGSCTACVAACPAGAIRTERSEFDHHGCYEMLSRFRKERNIGHHICG
                     VCVKICGGKR"
     gene            complement(2511..4373)
                     /gene="mnmG"
                     /locus_tag="PKJ17_00575"
     CDS             complement(2511..4373)
                     /gene="mnmG"
                     /locus_tag="PKJ17_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOD74486.1"
                     /translation="MDSYDIIVVGAGHAGCEAALAAARMGARTLLLTINLDHIAYMSC
                     NPAVGGLGKGHLVREIDALDGEMGVNTDEAGIQFKVLNMKKGPAVRATRIQTDMALYA
                     RRMKRRLEETEGLSIRQGVARNVLIEKGAAAGVETMLGERLAAPAVIVTTGTFLKGLI
                     HVGLEHFEGGRLGDMPSVGLSDNLRELGFQLGRLKTGTCPRLDGRTIDFSKMVPQPSD
                     DPPVPFSFMTGEVKNRLVPCYLAYTNERTHDVIRTGFDRSPLFTGVIKGTGVRYCPSI
                     EDKIVRFSDRSRHRIFIEPEGLDTVEYYPNGLSTSLPLDIQVAMLRTVEGLEEVRITR
                     PGYGIEYDFAYPTQLYPTLETKLVPNLYFAGQINGTTGYEEAAAQGLMAGINAALRLR
                     GEGDFVLGRHEAYIGVLIDDLVTKGVDEPYRMFTSRAEHRLLLREDNADLRLTEKGHG
                     IGVVGDGRCARVLEKKRKVGEAFSLLRGVRLRPTSKTQEMLREKGIEAITNPLTLEEL
                     LKRPDVSFDDLKGIHEGLRDIDSDIAYQVEVEVKYRGYTDRQLELVARTSKLDDRRIP
                     RELDYAGVSGLSREVVERFVKVRPSTLGQASRIPGVTPASITALLVHFKKKGML"
     gene            4513..4767
                     /locus_tag="PKJ17_00580"
     CDS             4513..4767
                     /locus_tag="PKJ17_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74487.1"
                     /translation="MEERAIGVVVRYFAKIGVAAIRITEGELKVGDRIRIKGHSTDLE
                     EEIESMQVEHENVQMVGAGTDVGIKVKERVREHDVIYLLT"
     gene            4906..5490
                     /locus_tag="PKJ17_00585"
     CDS             4906..5490
                     /locus_tag="PKJ17_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74488.1"
                     /translation="MEDKELKQRFLDIFEAATPILEDLKKGFFTQQVGLIQECEKKFG
                     DLLISRVPYVEKIIGEKEKSEADKKYVRLLPALQTIALAIENLMYKMEAKVAHNILFS
                     EKALSEVRDLYSLMQEQFRDTKDYVHTKNPGLHQAVKQHMEKMFRMIEELAQVHQNRL
                     ITGICIPQASYLYLDITDSMKRITRGLVDFADRV"
     gene            5590..6900
                     /locus_tag="PKJ17_00590"
     CDS             5590..6900
                     /locus_tag="PKJ17_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD74489.1"
                     /translation="MGGAIREIALGREPRDWDLALSDAGDLARLEEIFGRRSFLLGKK
                     PIQTHRLVAGETVIDITILEESIEKDLRRRDFTMNAVAFDIRRDRVIDPLSGLHDIEG
                     RLIRYPDRDTIASDPLRMLKAARHYAALTGFTLHPELIEAMASSRDLIRKTAPERIKY
                     ELDLIMVSQGVHRGMEILTRTGLIFEIVPELEALRVMDVEKNFSLETFGHTLLGFSFL
                     HDRGGRYLANRAAAKDVGYALLFHDLGKAHTYSYDEVKGLVHFFNHERRSQEMASAIM
                     ERLRFSTHEMKRISALIEHHMRIFLISSAEATEKAIRRLIYRLGDLTPELIALTLCDM
                     YGSSGGEENPSTRQVVQNCDSIMAAWHESKKKPLPRLLSGKDLLAMGFPEGPLLGRCL
                     DTVRDRQVAGEMKTREEALRFAEESLKKNEHAEPHPTEDPPDDP"
     gene            6982..8043
                     /locus_tag="PKJ17_00595"
     CDS             6982..8043
                     /locus_tag="PKJ17_00595"
                     /inference="COORDINATES: protein motif:HMM:NF017380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HOD74490.1"
                     /translation="MKRSVTSLPLHTGRAPAWLFERMKRLCGALIEVIVMEYGPRELL
                     TRLADPLWFQALGCAVGFDWHSSGLTTTLCGALKEGLASLGDDAPLAICGGKAKRAIR
                     TPDDIRGYGERWGVDVSGMVATSRLCAKIDNTAVQDGYSLYHHTFIFTPDGSWAIIQQ
                     GMNGERKDARRYQWLSREGLNVMSDPHTGITCDHTQEVLNFVAGRSADARSAVVDFAS
                     ENPDKMASTWEDIALSMPRRHYIRAEDVNAGRLLKAFNAIHEAHPRSFRDLLEIRGVG
                     PRTVGALALVSELVYGVPPSFADPARFSFAHGGKDGYPFPVDKSTYEHSIDFLKTCVD
                     KAKMGDREKLDAFRRLAKI"
BASE COUNT         1630 a         2587 c         2330 g         1663 t
ORIGIN      
        1 acccatggca tcgaggttct cgatggagca gatgatgacg acgttgtcgt tccggtcgcg
       61 catgacctcg agttcgaact ccttccagcc gatgacggat tcctcgatga gtatctccgt
      121 gatcagcgag ctttcaaggc ccctcacggc gagggtctcg aactcctcca cgttgtaggc
      181 cacggacgcg ccggtgccgc ccagagtgta actcggacgg atgatgagag gaaagcctat
      241 ctcgcgcgcg acgacccgcg cctcttccat cgtgtaggcg agaccgctcc tcgggacctc
      301 gaggcctatc ttctcgatgg ccgccttgaa ctcctccctg ccctcggcct tctttatggc
      361 acggtagtcg gccccgatga gctcgacacc gtacttctcg agtatgcccc gctcggcgag
      421 gatcgtcgcg aggttgaggc ccgtctgccc gcccagggtg ggaagaatga cgtcgggcct
      481 ttccgtcttt atgatctctt ccatgacctc cggtatcagg ggctccacgt agaccctgtc
      541 cgccatgtcg gggtccgtca tgatcgtcgc cgggttggag ttcacgagga tgaccctgta
      601 gccctcctcg cggatggcct tgcaggcctg gctccccgaa tagtcgaact cgcaggcctg
      661 gcctatgacg atgggccccg acccgatgat gagaacgctc ttgatgtccg ttcttttagg
      721 cattgccttc cttcctggag ctctccatca tgttgcggaa cttttcaaaa agaccgtagg
      781 aatcatgagg acccggcgac gcctcgggat ggtactggac ggagaaagcg gggtatgtct
      841 catgttcgat gccctcggcg gtgccgtcat tgaggttgac atgggtcacc cggacggcct
      901 gtcccctgat ggaatcgacg tcaacggcga acccgtggtt ctccgaggta atgtacacct
      961 tgcccgtcgc cgtctccctg atgggctggt tggctccgcg gtggccgaac ttgagcttgt
     1021 atgtcctgcc gccgagggcc agtcccagaa gctgctgacc caggcagatg ccgaatatgg
     1081 ggacccggcc gataagtttc tctatctccc tgatggcata gggaaccgcc tcggggtctc
     1141 ccggtccgtt ggagagaagg accccgtcgg gcctcatggc gacgatatcc tccgccttcg
     1201 tgcccgccgg cacgacggtg acgcggcaac ccttctgacg caatatcctc aggatgttcc
     1261 tcttgacgcc gaaatccatg acgacgcagt ggagcgcgtc gacggccggg tcgcccccgg
     1321 gtccgccttc aaactcatag ggcgcctgac aggtcacgcc gctcacgagg tcgacgccca
     1381 ctatcccccg ggaaccctgc gcctttctca cgagactgtc gcggtcgtgg tccgtggtgg
     1441 atatgatgcc cctcatgacg ccgcgcaccc tgaggtggcg ggtaagagcc ctcgtgtcta
     1501 tctcatcgac ggcgatgatg ttgtgcttgc ggaaataatc gccgaggtcg cccgtcgctc
     1561 tccagttgct cgttatccgg gagaactccc gcgcgatgaa gccttccacc ttgggcccgc
     1621 cggattcgac atcctcatcg ttgacgccgt agttgccgat cagcggatag gtcatcgtga
     1681 cgatctgtcc cttgtaggag ggatcggtca tgatctcctg gtatccggtc atgctcgtat
     1741 tgaagacgat ctctccctct ttctctccct caaggccgaa acctcttcct tcgaagaccg
     1801 tcccatcctc gagtgccacg aaggccttca tcgtttccct ccgcaaatct tcacgcagac
     1861 accgcatatg tgatggccga tgttgcgctc ttttctgaac ctgctcagca tctcatagca
     1921 cccgtggtgg tcgaattccg acctctccgt cctgatggcc cccgcggggc atgccgcgac
     1981 gcaggccgtg cagcttccac aatcccggcc cagttcgcgg cccgcctcga ggggcatgtc
     2041 cgtgaggacc gtgttgtacc tgacctgcga cccgaacagg gggtgaacga gcaggttgtt
     2101 gcgtccgatc catccgagac ctgccatgac cccaaccttc ttgtggttga tgtggccccg
     2161 ctggcgctgc cagtccacca cctgggacgc ggcgaagggg atcgccctga acccttccct
     2221 ttctatcgat ctgccgagaa aatatccgat catgtctagc ctgtagttga gctggcgata
     2281 gtggtggagg tacaactggg tcggaccgtc cttaagggtg ctgagcaccc ctaccgagag
     2341 tacaatgcca aaagagacgg caaatggtaa ctcatttgtc gcgacgtcgc cgatacccgt
     2401 tatctcctcg ccatagcagg acatatcggc gatcccgaag gtatcgacat cgaggccccg
     2461 gagccatcct tcgatcttct cgttatgccc tgcccttccc tcttctcctg tcatagcatt
     2521 cccttcttct tgaaatggac aagcagcgcc gtgatggaag cgggggtgac ccccgggatc
     2581 cgcgacgcct ggccgagcgt cgacggtctt accttcacga aacgttcgac cacctcccgt
     2641 gaaagccccg ataccccggc ataatccagt tccctcggta tccgcctgtc atcgagcttg
     2701 cttgtcctgg cgacgagttc gagctggcgg tcggtgtagc cgcggtattt cacctcaact
     2761 tccacctggt aggcgatgtc gctgtcgatg tcccgcaatc cctcgtgaat gcccttgaga
     2821 tcatcaaagg agacgtccgg cctcttgagg agctcctcga gggtcagcgg gttcgtgatg
     2881 gcctcgatcc ccttctcccg gagcatctcc tgcgtcttac tcgtgggcct cagcctcact
     2941 cccctgagaa gcgagaaggc ctctccgacc ttcctcttct tctccagcac ccgtgcacag
     3001 cgcccgtcac ccaccacacc gatcccgtgc cccttctccg tcaacctcag gtccgcgttg
     3061 tcttccctca gcaggagccg gtgctccgcc cgggacgtga acatccggta gggctcgtcg
     3121 acgcctttcg ttacgaggtc atctatgagg acaccgatgt acgcctcgtg tcggccaagc
     3181 acgaaatccc cctctccccg gagcctgagc gcggcattga tgcccgccat gagtccctgc
     3241 gcggcggcct cttcgtagcc cgtggtcccg tttatctggc ccgcgaagta gagattgggt
     3301 acaagcttcg tctccagtgt cggataaagc tgggtggggt aggcaaaatc gtactctatc
     3361 ccgtaacccg gccgggttat ccgcacctct tcgaggcctt cgacggtcct gagcatcgcg
     3421 acctggatat cgagggggag gctcgtcgac agaccgttgg gatagtactc gaccgtgtcg
     3481 agcccctcag gctcgatgaa gatgcggtgc ctcgacctgt cgctgaacct cactatcttg
     3541 tcctctatgg acggacagta cctgaccccc gttcccttga tgacgcccgt gaagaggggg
     3601 gaacgatcga aacccgtccg gatgacgtcg tgcgtcctct cgttcgtgta cgcgaggtag
     3661 cagggaacga gcctgttctt cacctccccg gtcatgaaag agaaggggac gggcgggtcg
     3721 tcgctcggct gcgggaccat ctttgagaaa tcgatcgtcc tgccgtcgag tctcggacag
     3781 gtgccggtct tcagccgacc aagctggaaa ccgagctccc gcaggttgtc cgacaggccc
     3841 acagagggca tgtcgcccag ccttcccccc tcgaaatgct cgaggccgac atggatgagc
     3901 cccttgagaa aggtccccgt cgtcacgatg accgccggcg ccgcgagacg ttccccgagc
     3961 atcgtctcca ctccggcggc ggcgcctttt tcgatgagca cattccgggc cactccctgg
     4021 cgtatcgaaa gaccctcggt ctcctcaagc cttctcttca ttctccgggc atagagggcc
     4081 atgtccgtct gtatcctcgt cgcccggacg gcggggccct tcttcatgtt gaggaccttg
     4141 aactggatgc ccgcctcatc ggtattgaca cccatttccc cgtccagggc atcgatctcc
     4201 ctgacgagat ggcccttacc gagaccgccg acggcagggt tgcaggacat gtaggcgata
     4261 tggtcgaggt tgatggtaag aagaagggtc cgcgcgccca tccgagctgc ggcaagcgcc
     4321 gcctcgcatc cggcatgtcc ggcccccact acgatgatat catacgaatc catatgcaac
     4381 gtccttaaaa gccgtatatt gtagcaggaa tcgagcctct tttccacccg cttcaggaat
     4441 aatgcattat cactgttcaa ttattcttat ccacatggta ggatggaaga aaaaggtgac
     4501 agggagggtc gcatggaaga aagggccatc ggtgtggttg tcaggtattt tgccaagatc
     4561 ggggttgcgg ccataaggat caccgaaggg gagttgaagg tcggtgaccg catcaggata
     4621 aagggccatt ccacggacct cgaagaggag atcgagtcga tgcaggtgga gcacgagaac
     4681 gtccagatgg tcggcgcggg caccgatgtg gggatcaagg tcaaggaaag ggtgcgcgag
     4741 cacgacgtca tctacctcct gacatagaca ggtcgcacaa ggcggacgcc agagggccgt
     4801 ccttgccgac cgccggcaga ggctgctttt ttgctcttca gggctgacag acagaccctt
     4861 ctgtgttata agatacggaa aagatctcac ggaaatggag gagcgatgga agacaaagag
     4921 ctgaagcagc gtttcctgga tattttcgag gcggcgaccc ccattcttga agacctgaag
     4981 aaagggttct tcacccagca ggtggggctc atacaggaat gcgagaagaa gttcggcgac
     5041 ctgctcatct cgcgcgttcc ctacgtcgaa aagatcatcg gggaaaagga aaagagcgag
     5101 gcggacaaga agtacgtgcg gctgcttccc gccctgcaga ccatcgccct ggccatcgag
     5161 aacctcatgt acaagatgga ggcgaaggtc gctcacaaca tccttttcag cgaaaaggcg
     5221 ctttccgagg tgcgtgacct gtatagcctc atgcaggagc agttccgcga cacgaaggac
     5281 tacgtgcaca cgaagaatcc gggtcttcac caggcggtca aacagcacat ggagaaaatg
     5341 ttcaggatga tcgaagagct tgcccaggtc catcagaaca ggcttatcac ggggatatgc
     5401 attccccagg catcctacct ctacctcgac atcacggatt ccatgaagag gatcacccgg
     5461 ggactcgtgg atttcgccga cagggtttag tggttgggaa tctctcctca ggaggttgcc
     5521 cgttcgatca ccgggggtga cgttggaggc agactcgctg catgcccctt caggggggag
     5581 gtttttctcg tgggaggagc catacgggag atcgccctcg gcagggaacc ccgggattgg
     5641 gacctcgccc tatccgatgc cggcgatctc gcgcgcctcg aggagatctt cggcaggcgg
     5701 tcctttctcc ttggaaagaa acccatacag acacaccggc tggtcgccgg agaaaccgtc
     5761 atcgacatca ccattctcga ggagagcatc gagaaagacc tccggagacg ggacttcacc
     5821 atgaacgccg tcgccttcga tatcaggcgg gaccgggtca tagacccttt gagcggactt
     5881 cacgatatcg aaggacggct tatccgttac cccgaccgcg acacgatcgc cagcgacccc
     5941 ttgaggatgc tcaaggccgc aaggcattac gccgccctca caggcttcac gctccatccc
     6001 gagctcatcg aggcaatggc ctcttcgcga gatctcattc gaaagacggc gccggagagg
     6061 atcaagtacg agctggacct tatcatggtc tcccagggcg tccatcgcgg gatggagatc
     6121 ctcacgagaa cgggcctgat cttcgagatc gttcccgagc ttgaggcgct gcgcgtcatg
     6181 gacgtggaga agaacttctc cctcgagacc ttcggccata cccttctggg attctccttc
     6241 ctccatgacc gcggcggccg ttacctggcg aaccgggcag ccgcgaaaga tgtcggctat
     6301 gccctgctct tccacgacct gggaaaggcc cacacctatt cttatgacga ggtcaagggt
     6361 ctcgtccatt tcttcaatca cgagcgccgg tctcaggaga tggcctcggc catcatggag
     6421 cggctgcgtt tcagcaccca tgagatgaaa cggatatcgg ccctcataga acaccatatg
     6481 cgcatctttc tcatcagctc cgcggaggcc acggaaaagg caataagacg cctcatctac
     6541 aggctgggcg acctcacccc ggagctcata gccctcactc tctgcgacat gtacggaagc
     6601 tcgggagggg aagagaaccc ttcgacgcgc caggtcgttc agaactgcga ttccatcatg
     6661 gcggcatggc atgaatcgaa aaaaaagccc ctcccaaggc tcctgtccgg caaagacctc
     6721 ctcgccatgg gctttcccga gggacccctt ctcggcagat gcctcgacac ggtccgcgac
     6781 agacaggtag caggcgaaat gaagacccgc gaagaggccc tgcgattcgc ggaagaatcc
     6841 ctgaaaaaga acgagcatgc ggaaccacac cccacggagg acccgccgga tgatccctga
     6901 aaacacgtcc tctaggtcct acacgtcctg ttcgtccaat atgtcccatc ggtcctatca
     6961 caccgcagcc cccctcaccc catgaaacgc tccgtcacat cactccccct tcataccggc
     7021 cgcgccccgg cgtggctctt cgaaaggatg aagcggctct gcggggccct tatcgaggtc
     7081 atcgtcatgg aatacggccc ccgcgagctt ctcacccggc ttgcggaccc cttgtggttc
     7141 caggctcttg ggtgcgccgt tggtttcgac tggcactcga gcgggttgac gacaacgctg
     7201 tgcggggccc tcaaggaagg gctggcatcc ctcggcgatg acgcgccgct cgccatatgc
     7261 ggcggcaagg caaaacgagc gatcaggaca cccgacgata tccggggcta cggcgagaga
     7321 tggggtgtcg atgtctccgg aatggtcgcg acgagcaggc tctgcgccaa gatagacaac
     7381 accgccgtgc aggacggcta cagtctctac caccatacct tcatcttcac tcccgacggt
     7441 tcctgggcca tcatccagca ggggatgaat ggcgaaagga aggacgcccg gcgttatcag
     7501 tggctctccc gggagggcct caatgtcatg tccgaccccc acacgggaat aacctgtgac
     7561 catacgcagg aagtcctcaa cttcgtcgcc ggccggagtg ccgatgccag gagcgccgtc
     7621 gtcgactttg cctcggaaaa ccccgacaag atggcctcga catgggaaga catcgccctt
     7681 tccatgccga gacgccacta catacgcgcc gaggatgtga acgcgggacg cctgctgaag
     7741 gccttcaacg ccatccacga ggcacacccg cggtccttcc gtgacctcct ggagatacgc
     7801 ggcgtcggcc cgcggaccgt gggcgccctt gcgctcgtct cggagctcgt ttacggcgtt
     7861 cccccgagct tcgctgaccc cgcccgtttc agcttcgccc acggcgggaa ggacggatac
     7921 cccttccccg tcgacaagtc cacctacgaa cattccatcg acttcctcaa gacctgcgta
     7981 gacaaggcaa aaatgggcga cagggaaaaa ctggacgcct tcagaagact ggcaaagata
     8041 taaaacggtt caaggtttca ggcggaagac gcggcacttc ccggtctcaa tctctttcaa
     8101 cctgaaaacg tgaaaccgcc tccaaggtct ttgacttgaa acctggaacc gtcttttcag
     8161 tattccggtt ctcccggttt cgtgatccgc ctgtactctt tcaggtcgag
//
LOCUS       DAOPOA010000015         8050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_10001_length_8050_cov_5.126079, whole genome shotgun sequence.
ACCESSION   DAOPOA010000015 DAOPOA010000000
VERSION     DAOPOA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10001_length_8050_cov_5.126079"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PKJ17_00600"
     CDS             <1..1067
                     /locus_tag="PKJ17_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74491.1"
                     /translation="NVPFRDIPIHSFFELGRTNFHTVLEREVDTLFDVSKHLWRVLLI
                     TDKTDLKRHYLLLSLHHSISDGTSAVRLAEELISCCTRILAGETPRTDPLPLRASLEE
                     LMASAGGTSRQSGTATGRPPGESGGGSMPYHEFQPPGRRHTRFRVHTMEVAETAVLEE
                     KRLRAGTSIHSALAASALTVMRKHFGDDVRLSVTTPVNARGLTGTAIGDGELWCMETP
                     VSIHGEDIGGRDTWQMARDYSDKLAFATAGGMALSACANTDPAEEIEGLRAARHFPLS
                     CLLLEEGRVGPDQKSPFVVENMGLVSGRQAADHIMAISAATAGDALSLTFAYTSPLLR
                     ESWADRFVSEFLHNLERMAK"
     gene            complement(1188..3236)
                     /locus_tag="PKJ17_00605"
     CDS             complement(1188..3236)
                     /locus_tag="PKJ17_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD74492.1"
                     /translation="MERDVLKEKIVAYFRGIAEDRGRKYLREVSKLSRIIAKARKTEE
                     FLGRYRVLVESFSDAIFMLDEDRKIISCNRAFRSLFQLKKDEIEKKTLRIITGGDDAC
                     DRLTATIDDIIAGNNRSVFSWEFVKKDGSSLTMEVTVSQVRASLSEEAAPIGGCVAIL
                     HDITDRIAREDALRAAEEKYRNIFENAAEGIFQVAVDGHYISANPALARIFGFDSPLQ
                     MTGDGDLRARKTFVDPEDYRRLNAILKARGFAENFESERYRTDGTRMWTSTNVRIVTD
                     RDNGTHYFEGTVRDITRRKNLESQLRHSQKMEAIGTLAGGVAHDFNNLLTALIGYGNL
                     LQVKMEKDNPLRVYVDHVLSSAKRAANLTNSLLAFSRKQAVNLEPNGVNEIITGVQRL
                     LERLLSEDIELHVQTSAREVTIMADRTQIEQILMNLATNARDAMTDNRVLTISTAHAS
                     IDKAFIHKHGYGKVGTYGRVSVTDTGHGMDENVKEHIFEPFFTTKEAGKGTGLGLSTV
                     YGIVKQHDGYITVESEPGKGTTFHLYFPSVRVKREKARQVESKMPSGTETILVAEDDR
                     ETLQLMKEVLKEYGYTVIGAADGDEAVRHFTEHRHRIGLVIIDVIMPKKNGMEVYSEI
                     SKLDPSVKVLFVSGHTRDVVLDRGVVDSRSNFLSKPIAPSLLLERLRTLLDTEAHSPL
                     "
     gene            complement(3242..4471)
                     /locus_tag="PKJ17_00610"
     CDS             complement(3242..4471)
                     /locus_tag="PKJ17_00610"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOD74493.1"
                     /translation="MTRIGIACDGGRGRAISGDALVRRAMASGGVTRPDLVLAFCSGR
                     NEPEAFFRGIRRVAGAHVPVVGGSAIGVISNDFLSYRDPVAAVAVLESDLIAFRLASA
                     GQVNENEREAGRKVAAAIARGPEDRLLLVFYDSIKVPPEEGSPPIMNSSTPLIAGIGE
                     VLEDAVPVIGAGLLGDYGFNRTVQFRGSSMASQSLVALMLGGDLSVYHRITHGCAPLD
                     GVYRRVTRVQGPLLYEIDGKPIVEMINELFDSEEWQEERPLDYLTLGVHCGERYERYR
                     ENQYVNRLILGVIPDRSGVMMFESDLKEGDEVQFMVRDAGRMVESALNNSRLLVDMID
                     KDGKVPLLALYIDCAGRAKDYSNTSVEEAGQVQKVLSERNIPLFGFYSGVELAPLLGR
                     TRGLDWTGVLTILARKP"
     gene            4709..5533
                     /gene="murI"
                     /locus_tag="PKJ17_00615"
     CDS             4709..5533
                     /gene="murI"
                     /locus_tag="PKJ17_00615"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOD74494.1"
                     /translation="MQKLAIGMFDSGVGGMTVLREVRELLPREHIIYFGDTARVPYGN
                     KSSRMVTKYALESALFLLTKGIKLLIIACNTSSAFALPILQRRMPVPVIGVIGPGAKE
                     AVRNTRNGRVGVIGTRTTIKSMAYDRSIKKLDPGIEVHSRACPLFVPIVEEGLEHDEI
                     AGLMARKYLGALKGSGIDVLVMGCTHYPVLEDVIRKTMGKDVKIVHSGKETAKEARAI
                     LEEKDLFNRRGRGKCEYFVTDAPESFHEIGGRILGEPLTHVRSLKGLDYRDLLFSS"
     gene            5530..7497
                     /locus_tag="PKJ17_00620"
     CDS             5530..7497
                     /locus_tag="PKJ17_00620"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74495.1"
                     /translation="MIADVALPIPVGRSFSYRVPGELAPHIFELGRVLVPFRYREAAG
                     VVIALREGEEEGLKSVAGVLDLFPLLSDELPALLRWSSSFYVTPAGIAFKHALPSAGD
                     LERFLSVESAGLPEVDGLPLRKAIRRLGRAGLLQLYREGALSLCETLTGKTFAPPPTA
                     PPGLGEIQREKALLIDSVEERLAYYNRVIPDHLGQGRNVLVLVPDYHAAGAWFAKRLR
                     EAYGPRVLWFSSGTPVKQRMETWFRVRREGGHVVLGNKNCVFLPLHDLSLVIVERCDD
                     DEYKNEEGLKFNAVRVAVERARLKGASIVLGCAASSIDAIHLARADGFRISCNEWPTR
                     GGYAERTTNVSSPSTGEIIDHLGAEAREAARQGGRVAIYTPRKDQGSYLKCHACRETL
                     SCPDCGEALTYDSKAGSLACPACRARLAYEGICPQCGSSAVGFSRIGVHFIEEHLRAA
                     LPDLSVTRITGDSLKKEIAAIRRQPQGAGSVLVGTQSLSKLYSLSIDRLLLAQWEELR
                     KMGGYRSEERTHQVLLNLLDALTPRSIISYSTRKEPADPREYLDISSFAERELRKRRD
                     AQFPPFVRVFLLEARAKTPARADAILKKVRTIIADGALEGALLGTLPLERPPYHIRKV
                     ILKGDEDLLRDTFEKFHDIPGVDIEADPTSF"
     gene            complement(7598..>8050)
                     /locus_tag="PKJ17_00625"
     CDS             complement(7598..>8050)
                     /locus_tag="PKJ17_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74496.1"
                     /translation="MKKALMIVLAILITVAFVTTVMAQDKKAATDKPAAATDKPVKEK
                     VAEKAKKAGVKKFAGDFVSMDEAAKTIVVKGETEMTFDVSKVKKMAKFAAGDKVNVVY
                     TEADGKNVAKAVTKAGAVKEKAKEKKEKKAEEKKDEKPAEKPAAPAKK"
BASE COUNT         1522 a         2584 c         2264 g         1680 t
ORIGIN      
        1 tcaacgtacc ttttcgcgac atccccatcc attcgttctt cgagctgggc aggaccaatt
       61 tccacactgt cctggaacgc gaagtggaca cccttttcga tgtttcaaaa cacctgtgga
      121 gggttcttct cataacggac aagaccgacc tcaagcgaca ctatctcctc ttgtccctgc
      181 accattcgat aagtgacgga acctccgcgg tccgcctggc cgaggagctg atctcctgct
      241 gcacgaggat cctcgccggc gagaccccgc gcacggaccc tcttcccctc cgtgcgtccc
      301 tcgaggagct tatggcgtcc gccgggggca cgagcaggca gtccggaacg gccacgggga
      361 gaccccccgg cgaaagcggc ggcggctcca tgccctacca cgaattccag cctccaggca
      421 ggcggcacac ccgttttcgc gtccacacca tggaggtggc cgagacggcc gtcctggaag
      481 agaagcgcct ccgcgccggg acgagtatcc actccgctct cgcggcgagc gctcttacgg
      541 tcatgcgcaa gcacttcggt gatgacgtcc gtctctccgt gaccaccccg gtcaacgcca
      601 ggggtctgac cgggacggcg atcggcgacg gcgagctctg gtgcatggag acacccgtca
      661 gcatacacgg tgaagacatc gggggccgcg acacgtggca gatggcccgg gattacagcg
      721 acaaactggc cttcgcaacg gccgggggaa tggccctctc cgcctgcgca aacacagacc
      781 cggcggaaga gatcgaaggg ctgcgtgcgg cccggcactt ccctctttcc tgtcttctcc
      841 ttgaagaagg acgcgtcgga ccggatcaga aaagtccttt cgtggtcgag aacatgggcc
      901 ttgtcagcgg acgacaggcc gcggaccata tcatggccat ctccgccgcg acggcgggag
      961 acgccctctc cctgaccttc gcctacacat ctccgctcct cagggaaagc tgggcggaca
     1021 ggttcgtcag cgaattcctg cacaacctcg agagaatggc aaaataaaaa aggacaggcg
     1081 gcggtgggtt tgagcagggt caatagggct gggatcggac ctgaacgcca gttgcggtca
     1141 aggggggtct ctggcccggc cgaggtccgg aggatcttcg acgaatgcta gagagggctg
     1201 tgcgcttccg tgtcgagaag ggtccgcagt ctctccagca gcagggaggg cgctatcggc
     1261 ttggagagaa agttactcct gctgtccacg acacccctgt cgagcaccac gtcccgggtg
     1321 tgaccgctga cgaaaaggac cttgacggaa gggtcgagtt tgcttatctc ggaatagacc
     1381 tccatgccgt tctttttcgg cattatcaca tcgatgatga caagtccgat cctgtgcctg
     1441 tgctcggtga aatgccggac cgcctcgtcc ccgtcagcgg cacctataac cgtgtagcca
     1501 tattccttga gcacctcctt catgagctgg agggtctccc gatcgtcctc cgccacgagg
     1561 atcgtttccg ttcctgaagg catcttcgat tccacctgcc ttgccttctc cctctttacc
     1621 cgcaccgacg ggaagtacag atggaacgtc gtcccctttc ccggctcgct ttccacagtg
     1681 atgtacccgt catgctgctt cacgatgccg tacaccgtcg aaagccccag tcccgtgccc
     1741 ttgcccgcct ccttcgttgt gaagaaaggc tcgaatatat gctccttgac gttctcgtcc
     1801 atgccgtggc ccgtatcggt cacgctaaca cgcccgtagg ttcccacctt gccgtaaccg
     1861 tgcttgtgga taaaggcctt gtcgattgac gcatgggcgg tggagatggt caggacgcgg
     1921 ttgtccgtca tggcgtcgcg ggcattcgtg gcaaggttca tgaggatctg ctcgatctgc
     1981 gtccggtctg ccattatggt gacctccctg gccgaggtct gcacgtgaag ctcgatgtcc
     2041 tcgctgagaa gcctttcgag aagcctctgc acccccgtta tgatctcgtt gacgccgttg
     2101 ggttcaaggt tcaccgcctg ttttctgctg aaggccagga gactgttcgt gaggttcgcc
     2161 gccctctttg ccgacgagag gacatggtcc acgtagacgc gcagcgggtt gtccttctcc
     2221 attttgacct ggaggaggtt gccgtatccg atgagggcgg tgagaagatt gttgaaatcg
     2281 tgggcgacac ccccggccag cgtgccgatg gcctccattt tctgggaatg ccgcagctgg
     2341 gactcgaggt tcttgcggcg ggtgatgtct ctgacagtcc cctcaaagta gtgggtgcca
     2401 ttgtctctat cggtcacgat gcgaacgttc gtcgatgtcc acatcctggt cccgtccgtg
     2461 cggtaccgtt ccgattcaaa gttctccgcg aatccccggg ccttcaggat ggcattgagg
     2521 cgccggtagt cctcgggatc gacgaatgtc ttccttgccc tcaagtcccc gtcccccgtc
     2581 atctgaaggg gcgagtcaaa tccgaagatc ctcgcgaggg ccgggttggc gctgatgtag
     2641 tggccgtcga cggcgacctg aaagatgccc tccgccgcgt tctcgaagat attgcggtac
     2701 ttctcctcgg cggccctgag cgcgtcctcc cgcgcgatgc ggtctgtgat gtcgtgaagg
     2761 atggcaacgc acccgccgat gggcgccgcc tcctcggaga gcgaagcgcg cacctgtgat
     2821 accgtcacct ccatggtgag ggaagagccg tcctttttca cgaattccca ggaaaagaca
     2881 gacctgttgt tccccgcgat gatatcgtcg atggtcgcgg taaggcggtc gcacgcgtca
     2941 tcaccccctg tgatgatgcg cagtgtcttt ttttctatct cgtccttttt caattgaaag
     3001 aggctgcgga aggcgcggtt gcaggagatg atcttcctgt cctcatcgag catgaatatc
     3061 gcgtcggaga agctctcgac gaggacccgg tagcgcccga gaaactcttc cgtctttctg
     3121 gcctttgcga tgatgcgcga tagcttgctt acttccctga gatatttcct ccctctgtcc
     3181 tcggcgatgc ccctgaagta ggcaacgatc ttttccttca agacatcccg ttccatggac
     3241 actagggttt cctcgcgagg atcgtgagaa cccccgtcca gtcgaggcct ctcgttcttc
     3301 ccagcagcgg tgcgagttcc acgcccgaat agaaaccgaa aagggggata ttgcgctcgc
     3361 tgaggacctt ctgcacctgg ccggcctcct cgacactcgt gttggagtag tcctttgccc
     3421 ggcccgcgca atcgatgtac agggcaagaa ggggtacctt tccatccttg tctatcatgt
     3481 ccacgaggag acgggaattg ttcagagcgg actccaccat ccggccggca tcccgtacca
     3541 tgaactggac ctcgtcaccc tctttgaggt cggattcgaa catcatcacc cctgacctgt
     3601 cgggaatgac gcccagaatc agtctgttca cgtactggtt ctcccgatac ctttcgtatc
     3661 tctctccaca gtggaccccg agggtgagat aatcaagggg ccgctcctcc tgccattcct
     3721 cgctgtcgaa gagttcgttt atcatctcca ctataggttt gccgtctatc tcgtacagga
     3781 gcgggccctg caccctcgtc accctgcggt agaccccgtc gagtggggcg cagccgtgcg
     3841 ttatccgatg gtagacggag agatcgccgc ccagcatgag agccacgagg ctctgcgatg
     3901 ccatcgagga accgcggaac tggactgtcc tgttgaaacc gtaatcaccc aagagtcccg
     3961 ccccgatgac gggaacggca tcctccagga cctctccgat cccggctatg agcggcgtcg
     4021 atgaattcat gatcggggga gatccttcct cgggtggaac cttgatcgaa tcgtagaaga
     4081 caaggagaag cctgtcttcc ggcccgcggg cgatagcggc ggcgaccttc ctgccggcct
     4141 ctctttcgtt ctcgtttacc tgcccggccg aagccagtct gaaggcgatg aggtccgact
     4201 cgagcaccgc caccgctgcc acagggtccc tgtaggagag aaagtcgttg gatatcacgc
     4261 cgatcgcgga ccctccgaca acggggacgt gggctcccgc aacgcgtctt ataccgcgga
     4321 agaaagcctc gggttcgttt ctgccggagc agaaggcgag gaccaggtcg ggcctcgtta
     4381 cgccgcccga cgccattgcg cgcctgacaa gggcatctcc ggaaatggcc cggcccctgc
     4441 ccccgtcgca ggcaatacct atcctcgtca tcgtctccgt ttcaacccct ttgtttcccg
     4501 tgccgtcccc cggggcgtca cattccccct cgacacgggg gcactacagt ggcggtccgc
     4561 ggtccacagc tgttacacaa ggcagattgt cagggaatcg ctttattcta tttaccagca
     4621 ttggatttaa aaaggcaagc aaaaaggctc ctctgcccgc gggcttattg ccttgacatt
     4681 tcacccccat ctctcctaat ctgtagccat gcaaaaactg gccatcggca tgttcgactc
     4741 cggggtggga ggcatgaccg tcctcaggga ggtcagggaa cttctccccc gcgaacacat
     4801 catctatttc ggagataccg ccagggttcc ttacgggaac aagtcctccc ggatggtgac
     4861 gaaatatgcc ctggaaagcg cccttttcct tctcacgaag ggcatcaagc tcctcatcat
     4921 cgcctgcaac acctcgtctg cattcgcgct cccgatactg caaagacgga tgcccgttcc
     4981 cgtcatcggt gtcatcgggc ccggcgccaa agaggcggtg agaaacacca gaaacgggag
     5041 ggtcggggtc atcggcacga ggaccacgat caagagcatg gcctacgacc ggtcgatcaa
     5101 gaagctcgat cccggcatag aggtccactc ccgggcgtgc cccctcttcg ttcccatagt
     5161 ggaggaaggc ctcgaacatg atgagatagc aggcctcatg gcccgaaagt accttggcgc
     5221 cctcaagggc tccgggatcg atgtcctcgt catgggatgc acccactacc ccgtcctcga
     5281 ggacgtcatc aggaagacca tgggtaagga cgtgaagatc gtgcactccg gaaaggaaac
     5341 cgctaaggag gcccgcgcca tcctcgaaga gaaagacctc ttcaacagac gggggcgcgg
     5401 caagtgcgag tactttgtca cggacgcccc cgaatcgttc cacgagatag ggggcaggat
     5461 cctcggtgag ccgctgacac acgtgaggtc cctgaagggc ctcgactacc gcgatctcct
     5521 tttctcttca tgatcgccga tgtagccctc ccgatacccg tcggcaggtc cttctcctac
     5581 agagtccccg gtgaactcgc cccccatatc ttcgaactcg ggcgtgtcct cgttcctttc
     5641 cggtacaggg aggccgccgg cgtcgtcatc gccctcaggg aaggcgaaga ggaggggctc
     5701 aagtccgtcg cgggtgtgct cgatcttttt ccccttctct ccgacgagct tccggcattg
     5761 ctccgatggt catcgtcatt ctatgtcacg cccgcaggga tcgcgttcaa acacgccctc
     5821 ccctccgcgg gagacctcga acgcttcctc tccgtcgagt ccgccggcct ccccgaagtg
     5881 gacggtctgc ccctcaggaa ggccatcagg agactcggca gggcgggact cctgcaactg
     5941 tacagggagg gggcactcag tctttgcgag acccttacgg gaaaaacctt tgccccgccc
     6001 cccacggctc ccccggggtt gggggagatc caaagggaaa aggcccttct catcgattcc
     6061 gtcgaagaac ggctggcgta ctacaacagg gtcataccgg accacctggg gcagggcagg
     6121 aacgtcctcg tccttgtccc cgattaccat gccgccgggg cgtggttcgc gaagagactc
     6181 agggaagcct atggtccccg tgttctgtgg ttctcctccg gtacgcccgt gaagcagagg
     6241 atggaaacct ggttcagggt ccgtcgcgag ggcgggcacg tggtcctcgg gaacaagaac
     6301 tgcgtctttc tccctcttca cgacctctct ctcgtcatcg tcgagagatg cgacgatgac
     6361 gagtacaaga acgaggaggg gttgaagttc aacgccgtgc gggttgccgt cgaaagggcg
     6421 cggctcaagg gggcctccat cgtcctcggc tgtgccgcct cctccatcga cgcgattcat
     6481 ctcgcccggg cagacggttt ccggatcagt tgcaatgaat ggcccacccg gggaggctat
     6541 gcggaaagaa cgacgaacgt ctcctcccct tcgacgggcg agatcatcga ccacctcggc
     6601 gccgaggcca gggaagcggc ccggcaaggc gggcgcgtcg ccatctacac accgcgcaag
     6661 gaccagggct cgtacctcaa atgccatgcc tgcagggaga ccctttcctg tccggactgc
     6721 ggggaagccc tcacctacga cagcaaggcg ggcagcctcg cctgtcccgc ctgccgggcc
     6781 cggctggcct atgagggcat ctgtccccag tgtggcagca gcgccgtcgg cttctcccgg
     6841 ataggagtac acttcatcga ggaacacctg cgcgccgcct tgccggacct cagcgtcacc
     6901 cgcataacgg gagactccct caagaaagag atagccgcca tccgcagaca gccccagggc
     6961 gcaggctccg tcctcgtcgg tacccaatcc ctctccaagc tctacagcct ctccatcgac
     7021 agactcctcc tcgcacaatg ggaggaactg cgaaagatgg gaggatacag atcggaagaa
     7081 aggacccatc aggtccttct caatctcctc gacgccctca caccccggtc catcatctcc
     7141 tattccacca gaaaagagcc cgctgacccc cgcgagtatc tcgatatttc ctcatttgcc
     7201 gaacgggaac tcaggaaacg cagggatgct cagttcccgc catttgtccg ggtctttctc
     7261 cttgaggccc gcgccaagac ccccgcccgg gccgacgcca ttctcaagaa ggtccgcacg
     7321 atcatagccg atggagccct cgaaggcgcg ctcctcggca ccttacccct cgaaaggccc
     7381 ccctatcaca tacgcaaggt gatactcaag ggagatgaag acctcctcag ggacaccttc
     7441 gaaaagttcc acgacatccc cggagtcgac atcgaagctg acccgaccag cttctgaaag
     7501 accgcttgag ccgcttgagc cgtttgagtg gcttgagcga aaagaaccaa aaaaaaggag
     7561 gcttttggcc tccctttttt ctgttcgcga tgcggaacta cttcttggcc ggagccgcgg
     7621 gcttctcagc cggcttctcg tccttcttct cttccgcttt cttctctttc ttctctttgg
     7681 ccttttcctt cactgcgccg gctttcgtca ctgccttggc aacgttcttg ccgtctgcct
     7741 cggtgtagac tacgttgact ttgtcgccag ccgcgaactt tgccatcttc ttcaccttgg
     7801 aaacgtcgaa ggtcatctcg gtctcaccct tgacgactat tgtcttcgcc gcctcgtcca
     7861 tggagacgaa gtcgcctgcg aacttcttca cgccggcttt cttggctttc tcggccacct
     7921 tctccttcac gggcttgtcc gtcgctgcgg ccggtttgtc tgtcgcggct ttcttatcct
     7981 gcgccataac ggtcgtaacg aaggcaacgg tgatcaggat cgcgagaacg atcatcaatg
     8041 ctttcttcat
//
LOCUS       DAOPOA010000016         7954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_10175_length_7954_cov_3.761489, whole genome shotgun sequence.
ACCESSION   DAOPOA010000016 DAOPOA010000000
VERSION     DAOPOA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10175_length_7954_cov_3.761489"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /gene="purB"
                     /locus_tag="PKJ17_00630"
     CDS             complement(<1..1110)
                     /gene="purB"
                     /locus_tag="PKJ17_00630"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOD74497.1"
                     /translation="MIERYTRERMARVWTDENKYRKWLDIEVLICEAYTKLGLIPEED
                     MKNIREKANFEVARVLEIEERTRHDVVAFIENVAEYVGPSSKYIHMGVTSSDILDTSF
                     SCLLNEAADILIEDVVALMEVLKEKAYACKDMPAIGRTHGIHAEPVTFGLKITHFYDE
                     MKRNLERMTAAKERIRHGKISGAVGTYAHVPPSVEEYVCEKLGLKVCPISSQIIPRDY
                     YAEFFTAIALTGSTIERMSMEVRNLQRTEVGEAEEFFRKGQTGSSAMPHKRNPIASEN
                     LCGMARLLHGFAVTAMENIPLWHERDISHSSAERVMAPDATIALDYMLERVKNVFKNL
                     LVYPEKMQKNLDITRGLYHSEAILIALIRKGLTRQE"
     gene            complement(1601..2068)
                     /locus_tag="PKJ17_00635"
     CDS             complement(1601..2068)
                     /locus_tag="PKJ17_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74498.1"
                     /translation="MWNWKPFFDDESISAFCDLDQIVDTEADDDGLYASPDCYRPLPL
                     RFGVFLSIVLKKKDDMARYLEERKARSLPLKGYKSYRYSLCLAEIDARTMRCRVLPAG
                     DYDSKDRELGDSCIVTDITPPILPGITDEWKPIRSKRSHPMVRALAKMFFPKE"
     gene            complement(2075..2572)
                     /locus_tag="PKJ17_00640"
     CDS             complement(2075..2572)
                     /locus_tag="PKJ17_00640"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOD74499.1"
                     /translation="MAGKIPIPSTTRKGAADVARRLKTFDRKESFAVLATDDRGKPYT
                     SLISFALTPDLRQIIFATPKGTRKFRNLTGTKDVALLIDNRSDQKKGFVQTQAITVIG
                     TARPVRRGRAWLEYAGIFLKKHPELEEFLNASTTALVVVDVTSCVHVGHFQTVTVADW
                     ANKES"
     gene            complement(2591..3952)
                     /locus_tag="PKJ17_00645"
     CDS             complement(2591..3952)
                     /locus_tag="PKJ17_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOD74500.1"
                     /translation="MNVLPQRVEYIREGRPGRGPVAYWMSRDQRSADNWALLFAQETA
                     IAHTLPLVVVFCLSDSFPGATLRQYGFMLRGLEKVSRSLGALSIPFFLLPGEPADTLP
                     AFIKGHDISFLVADFDPHRVKRQWKEAVIRRTKVPVYEVDAHNIVPCRAASDKREYGA
                     HTIRRKIRRLLPVFLDDMPVLQRHPWTSELKGDGVDAKTLLDTLRTDRSVEEVDWLTP
                     GEDEAHIVLRSFTEEKLDRYETDRNDPVKDCQSNLSPYLHFGQISAQRVALEVRDCPA
                     PIGAKDAFLEEHIVRRELADNYCLHTPNYDRFEAFPEWAKKTLNDHRPDRRPYLYSLR
                     ELENAETHDELWNAAQTQMAKTGKMHGYLRMYWAKKILEWTASPEEAISAAVCLNDRY
                     ELDGRDPNGYTGIAWSIGGVHDRPWGERNVFGKIRYMSYNGCASKFSVKRYIEAVRSG
                     TRG"
     gene            4036..4425
                     /locus_tag="PKJ17_00650"
     CDS             4036..4425
                     /locus_tag="PKJ17_00650"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOD74501.1"
                     /translation="MKLSEYFENTLGRGVLATADAKGIVDAAVYSRPHFVDEETAVWI
                     MTDRLTHANLQSNPHAAYIFAEAKENAFVGKRLYLTKIREESDPAKIEQFRWRKTYRI
                     PDEQKDEKRFVVYFHIDRVLPLIGDKT"
     gene            4429..4599
                     /locus_tag="PKJ17_00655"
     CDS             4429..4599
                     /locus_tag="PKJ17_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74502.1"
                     /translation="MKDHDHDHARPSARAFLTNLKADMPLWRKVWLLLRNNTKKIVTM
                     RGCCGHPGEPGC"
     gene            complement(4908..6113)
                     /locus_tag="PKJ17_00660"
     CDS             complement(4908..6113)
                     /locus_tag="PKJ17_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD74503.1"
                     /translation="MISYEQALQTVLENVHVLRPERRSILKGTGRACAPPNFLLGAAC
                     VLIVAALTVLAPVGGLQARELTDIFGRRIAVPDRIRHAWATSPPATYMLYAMDPGVLA
                     GLNTPVREWERNYFRRDIQQLPVLGGWYGQGNVPNIEMVLKVNPEIIIATSLNSSMEE
                     RKSRTLRDVPIPVVGVKMERLSDYPDAFTFLGEALGRRERGKKLSDYARKTIGKMSAI
                     VEAVPPRQRVSVYYAEAEDGLSTACHGSMHAELIALAGGRNVHRCPAKDVYGMEKVSM
                     EQVLLYDPEVILVFEPLFFKGIFSDPRWRQIRAVKERKVYLIPRQPFNWFDRPPSFMR
                     LLGLTWLTNLLHPDLYPVDIVKETQAFFRLFLGIDLTGREAMEIIHPRGDVSGHGTGI
                     TGHRPHRTR"
     gene            complement(6118..6960)
                     /locus_tag="PKJ17_00665"
     CDS             complement(6118..6960)
                     /locus_tag="PKJ17_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD74504.1"
                     /translation="MALMEVRRVTFAYKDKPVLKDVSVEICEGEVVTLLGPNGSGKST
                     LLKTIIGLIRPGQGTVRLAGRDISSIPQKELARQIAYVPQFHRSAFPYRVFDVILMGR
                     MPHKTFFFRYSQEDIDIARTVMDRLSIAHLAGRPYTEISGGERQLTLIARALAQGARV
                     FVMDEPASGLDYGNQLKLLEHITDLSGEGYTFIKSTHSPEHALWIAHRAVMIRKGAII
                     ADGCCDDVVNSETLLQLYNTRVKVVRLNGSLRVCVPEMLKDNGAEEGTGRQPMFVRSG
                     TSRR"
     gene            complement(6960..7694)
                     /locus_tag="PKJ17_00670"
     CDS             complement(6960..7694)
                     /locus_tag="PKJ17_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOD74505.1"
                     /translation="MSPGILGVLAGASFGAAVGMVLSKSWFFVQASCFLFGFLAVAAA
                     VGVARLYKGNSIILLILGGVISGALFTSLLSIVKYLADPYNQLPAIVYWLMGGLTLVD
                     AGVIRNTAIPVVAGVGLVISLSGYLNVLSMGDEEARSMGVPVERIRIILIVIATMLSA
                     ITVVLAGMVGWVGLIIPHISRMIVGPDNRVLIPVSALVGALYLVAVDDVSRLLFTTEI
                     PLGIITSLVGIPFFAVVLKRAGKGWS"
     gene            7703..>7954
                     /locus_tag="PKJ17_00675"
     CDS             7703..>7954
                     /locus_tag="PKJ17_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74506.1"
                     /translation="MNIAWKAAPDMDSDAPTKSAAMMRGRRILRRMFSRSCLSTDRFT
                     PALNIFQRKNPQTSSMLTGYFPSTSEMSTAMTRAQASPIM"
BASE COUNT         1573 a         2334 c         2389 g         1658 t
ORIGIN      
        1 ttcctgtcgc gtgaggccct tcctgataag ggcgatgagt atcgcctccg agtgatagag
       61 gccgcgggtg atgtcgaggt tcttttgcat cttctcgggg tagacgagga ggttcttgaa
      121 gacgttcttc accctttcga gcatgtagtc gagggcgatg gtggcgtcgg gggccatgac
      181 gcgctccgcc gacgagtgac tgatgtccct ctcgtgccac agggggatat tctccatcgc
      241 cgtcacggca aagccatgca gaagccgcgc catgccgcag aggttctccg atgcgatggg
      301 gttcctcttg tggggcattg ccgaagatcc cgtctgcccc ttgcggaaga actcctccgc
      361 ttcgcccacc tcggtccgct gaaggttgcg gacctccatg gacatccgtt caatggtgga
      421 ccccgtgagc gctatggcag tgaagaactc cgcgtagtag tcgcggggga tgatctgcga
      481 tgatatgggg cacaccttga gacccagctt ctcgcagacg tattcctcga cggagggcgg
      541 gacgtgcgcg taggtgccga cggcgcccga tatctttcca tggcggatac gttccttcgc
      601 ggccgtcatc ctttcaaggt tccgtttcat ctcgtcgtag aaatgggtta tcttgaggcc
      661 gaaagtgacc ggctcggcgt ggatgccatg ggtcctgccg atggcgggca tgtccttgca
      721 ggcgtaggcc ttctccttga ggacctccat gagcgcgacg acgtcctcga tgaggatgtc
      781 cgccgcttcg ttgagaaggc aggagaagga cgtgtccagt atgtccgacg aggtgacgcc
      841 catgtggata tacttggagg agggtccgac gtactcagcg acgttctcga tgaaggccac
      901 cacatcgtgg cgcgtccgct cttcgatctc gagcaccctg gcgacttcga agttcgcctt
      961 ctccctgatg ttcttcatgt cttcttcagg gatgaggccg agcttcgtat acgcctcgca
     1021 gatgaggacc tctatgtcga gccacttccg gtacttgttc tcatccgtcc agacccgagc
     1081 catccgttcc cgcgtatacc gttcgatcat gcccactata taacccgacg gaaggaatga
     1141 tgtcaatttg aaaacaagga cattatctct cgcccgttcg tcgcgttcgc tcctctctca
     1201 agaccgcaga ggacgcaaag agagacggaa aagagagaaa agactgccgc cggatctcgg
     1261 agcctcccaa aatccggcgg ccttctcgtc ccgccgtccc ggcgggagag agaagaatat
     1321 ccttattctg aagagactat tctcccccca tcccccctgt agtcatatgt cttataggtc
     1381 ctgtacccca tactccaacc cccatactac ctcaccccac aactcctctc ctccccccat
     1441 cagccccacc tgaaaagaaa actcttcttt tgcctcccgt cgcaggcggg atgcaaagac
     1501 ggccagatcc aggcgggcgc cgggatctgg ccgagtcttc tctctgcgtg ctctcttcat
     1561 gagctctgcg ccctcgagcg aagcgggcgt gagatggtct ctattctttc gggaagaaca
     1621 tcttcgccag ggcgcggacc atgggatggg agcgttttga gcgtatgggt ttccattcgt
     1681 cggttatgcc ggggaggatg ggcggggtga tgtcggtgac gatgcagctg tcgccgagct
     1741 cgcggtcctt cgaatcgtaa tcacccgcgg gcaggacgcg gcagcgcatg gtgcgggcgt
     1801 ctatttccgc gaggcagagg gaatagcggt atgacttgta ccctttgagg ggcagggaac
     1861 gggcctttcg ctcctcgaga tagcgcgcca tgtcgtcctt cttcttgagg acgatggaca
     1921 ggaacacgcc gaagcgaagg ggcaggggac ggtagcagtc cggcgacgcg tacaggccgt
     1981 cgtcgtcggc ttcggtgtcg acgatctggt ccaggtcgca aaaagcgctg atgctttcgt
     2041 cgtcaaagaa tggtttccag ttccacatga tccgttaact ctccttgttc gcccagtctg
     2101 ccacggtgac ggtctgaaag tgacccacgt ggacgcagct cgtcacgtcg acaacgacga
     2161 gcgccgtcgt cgaggcgttg agaaactcct ccagttcagg atgctttttc aggaagatcc
     2221 ccgcgtattc cagccatgcc ctgccacggc ggacaggcct tgccgtgccg atgaccgtga
     2281 tggcctgcgt ctggacgaag cctttcttct gatcggagcg gttgtcgatc aagagtgcca
     2341 cgtctttcgt gcccgtgagg ttcctgaact tccgggtgcc cttcggcgtc gcgaatatga
     2401 tctgccgcag gtcgggcgtc agggcaaagg aaatgaggga cgtgtagggt ttcccccgat
     2461 catccgttgc caggaccgcg aaggactcct tcctgtcgaa ggttttgaga cgccttgcca
     2521 catccgcggc gcctttccgt gtggtgctcg gtatgggtat ctttcctgcc atatcctgct
     2581 ccctcctctt ttaaccccgg gtgccggacc tgaccgcctc tatgtagcgt ttcacgctga
     2641 atttcgacgc gcacccatta taactcatgt agcgaatttt gccaaagaca ttccgttcgc
     2701 cccagggcct gtcgtgcacc ccgccgatgc tccaggcgat gcccgtatac ccgttcgggt
     2761 cgcggccgtc aagttcatac cgatcgttga gacagacagc agccgatatc gcctcttcag
     2821 gggaggccgt ccattccagt atcttcttcg cccagtacat cctcaggtag ccgtgcatct
     2881 ttcccgtctt tgccatctgt gtctgcgccg cgttccagag ttcgtcatgg gtctcagcgt
     2941 tctcaagttc ccgcagggaa tagaggtagg gacgcctgtc gggcctgtgg tcgttgaggg
     3001 tcttcttcgc ccattcagga aaggcctcga agcggtcata gttgggggta tggaggcagt
     3061 agttgtctgc aagctccctg cggacgatgt gttcctcgag aaacgcgtcc ttcgccccga
     3121 tgggggcagg gcagtccctg acctcgagcg ccacccgctg ggccgatatc tgtccgaaat
     3181 gaaggtaggg cgagaggttc gactgacagt ctttgacagg atcgttcctg tccgtctcgt
     3241 atcggtcgag tttctcctcg gtgaatgacc gcagcacgat gtgtgcctcg tcctcccccg
     3301 gtgtgagcca atcgacctct tccacggagc ggtcggtccg tagggtatcg agcagcgtct
     3361 tcgcatcaac gccgtcgccc ttgagttctg aggtccaggg gtgcctttga aggacgggca
     3421 tatcgtccag aaagactggc aggagccgcc gtatctttct ccttatggtg tgggcgccat
     3481 actcgcgctt gtcggaggcc gcgcggcagg gaacgatatt gtgggcgtcg acctcgtaca
     3541 cggggacctt cgtcctgcgg atgaccgcct ccttccattg ccgcttcacg cgatgaggat
     3601 cgaagtccgc aacgaggaat gagatatcat ggcctttgat aaatgcaggc agggtgtctg
     3661 ccggctctcc gggaaggaga aagaagggga tggaaagcgc cccgagggag cgggagacct
     3721 tctccaggcc gcggagcatg aagccgtatt gcctcagggt cgcgccggga aaggaatcgg
     3781 aaagacagaa tacgacgaca aggggaaggg tgtgcgcaat agccgtctcc tgcgcgaaga
     3841 ggagcgccca gttgtcggcg ctgcgttggt cgcggctcat ccagtacgcc acgggccccc
     3901 ggcccggccg cccctccctg atatattcga ccctctgcgg taacacgttc atctgctcct
     3961 tctgttattt tataacaaag gagcggttct ttgctataat gggaaaacat acagcacaga
     4021 aagagaggag ggtccatgaa gctcagcgag tattttgaga acactttggg aaggggcgtt
     4081 ctcgccacgg cggacgcgaa gggcatcgtt gacgcggcgg tctattcccg gccccatttc
     4141 gtcgacgagg aaacggcggt gtggataatg acggaccggc tgacacacgc caacctccag
     4201 tcgaaccccc atgccgccta catcttcgcg gaggcgaagg agaacgcctt cgtggggaag
     4261 cgtctctatc tcacgaagat ccgtgaagag tcggatcccg cgaagatcga gcagttccgg
     4321 tggcgcaaga cgtataggat tccggacgag cagaaggacg aaaagagatt cgtagtctac
     4381 ttccacatcg acagggtcct gccgctcatc ggtgacaaaa cttaggccgt gaaggatcac
     4441 gaccacgacc atgcaaggcc gagcgcgagg gccttcctga cgaacctcaa ggccgatatg
     4501 cccctgtggc gaaaggtgtg gcttctcctg cgcaacaaca cgaagaagat cgtcacgatg
     4561 cgcgggtgct gcggccaccc cggtgaaccc ggctgttgac agaccgatga cacgtccacc
     4621 ggtcggtgaa cgaagagagt gcctgaaaag agatatggcc gtccggccac atgcacgcga
     4681 cgaccacatc cccaatagaa aggattatga gaggagtagc gttataaaga gatcaacctc
     4741 ctttgaaggt cggttctcgt aatattatcc ggtgtacccg tgttcacctg ttgtctcgcc
     4801 gggtcgcgtc catcagaatt ttccctttca atcatcatat agtgttatat acatatggta
     4861 ataacgctgt caagcttttt ttgagagttc acattgacag gtcttatcta gcgtgtcctg
     4921 tgcggtctgt gaccggtgat ccctgtcccg tgccctgaga cgtctccccg agggtgtatt
     4981 atctccatgg cctctctgcc ggtcaggtca atgccgagaa agagacggaa aaaggcctgc
     5041 gtttccttca cgatgtccac gggatacagg tcgggatgaa gaaggttcgt cagccacgtg
     5101 agaccgagca ggcgcatgaa ggacggaggg cggtcgaacc agttgaaggg ttgtctcggt
     5161 atgagataga cctttcgctc cttcacggcc cttatctgcc gccacctggg gtcggagaaa
     5221 atgcccttga aaaaaagagg ctcgaagacc aggatgacct ccgggtcgta gaggagcacc
     5281 tgttccatgg aaaccttctc catgccgtaa acgtccttcg ccgggcagcg gtgaacgttg
     5341 cgcccccccg ccagtgctat gagctccgcg tgcatggacc cgtggcatgc ggtgctgagc
     5401 ccgtcctccg cttcagcata atagaccgat accctctgcc gcggcggtac ggcctcaacg
     5461 atcgcagaca tcttcccgat cgtcttccgg gcgtagtcgc tcagcttttt cccgcgctcc
     5521 cggcggccga gggcctcacc aaggaacgtg aaggcgtcgg gataatcgga gagcctctcc
     5581 attttcacgc ccaccaccgg tattggcacg tcccgtaacg tccggctctt cctttcttcc
     5641 atggaggagt tgaggctcgt cgcgatgatg atctcgggat tcaccttcag gaccatctcg
     5701 atgttgggca cattgccctg cccgtaccat ccgccgagga cgggcaattg ctgtatgtcc
     5761 ctgcgaaagt agttcctttc ccattctctg accggcgtgt tcaggccggc gaggacgccg
     5821 gggtccatgg cgtagagcat gtacgtggcc ggcggcgatg tggcccaggc gtgccgtatg
     5881 cggtcaggaa cggcgatcct cctgccgaat atgtcggtaa gctctctcgc ctgcaggccg
     5941 cctaccggcg cgaggaccgt cagcgcggcg acaataagca cgcacgcggc cccgagaagg
     6001 aaattgggcg gtgcgcacgc gcggccggtg ccttttagga tggaccgtct ttccggcctg
     6061 aggacgtgca cgttctccaa caccgtttgc agcgcctgct cgtaactgat cattgcctca
     6121 cctccgcgaa gttcccgaac gaacgaacat gggctggcgc cctgttccct cctcggcgcc
     6181 gttgtccttc agcatctcgg gcacacagac gcgcagcgat ccgttcaatc tgaccacctt
     6241 gaccctggtg ttataaagct gcagcagggt ttcgctgttc acaacatcgt cgcagcaccc
     6301 gtcggcgatg atcgccccct tccggatcat aacggcgcgg tgtgctatcc agagggcgtg
     6361 ctccggtgag tgggtcgact ttatgaaggt gtatccctcc ccggacaggt ccgtgatgtg
     6421 ttcgagcaat ttgagctgat tgccgtagtc gaggccgctc gcgggctcgt ccatgacaaa
     6481 gacgcgggcg ccctgcgcca gggcccgggc aatgagggtg agctgtctct cgcccccgct
     6541 tatctccgtg tagggacgcc cggcgagatg cgcgatggag agtctatcca taacggtccg
     6601 cgcgatgtcg atgtcttcct gggagtaccg gaagaagaac gtcttgtggg gcatgcggcc
     6661 catgaggatc acatcgaaga ccctgtaggg aaaggcgctc ctgtggaact gggggacgta
     6721 ggctatctgc ctggccaatt ccttctgcgg aatggacgag atgtcccgtc ccgcgaggcg
     6781 gacggtaccc tgacccggcc tgatgaggcc gatgatcgtc ttgaggaggg tgcttttccc
     6841 gctcccgttg ggaccgagga gagtgaccac ctctccctcg cagatctcca cgctgacgtc
     6901 cttcaggacc ggtttgtcct tgtacgcgaa ggtgacgcgc ctgacctcca tgagagccat
     6961 caactccacc cctttccggc cctcttgagg accacggcga agaaaggtat gccgacgagg
     7021 gaggtgatga tccccagggg tatctccgtt gtgaagagaa ggcgcgaaac gtcatcaacg
     7081 gcaacgaggt aaagcgcccc gacgagggcg ctcaccggga tgagcacgcg gttgtccggt
     7141 cctacgatca tcctcgagat gtggggtatg atgagcccga cccagccgac catcccggca
     7201 agcacgacgg tgatggcgct cagcatcgtg gcaatgacaa tgagaatgat ccggatccgt
     7261 tcgacgggaa cgcccatgga ccgcgcctcc tcgtcaccca tggagaggac gttgaggtac
     7321 cccgaaagcg agatgacgag accgacaccg gcaacgacgg ggatggccgt gttcctgatg
     7381 acgcccgcgt caacaagggt gaggccgccc attagccagt agactatcgc gggaagctgg
     7441 ttgtaagggt cggcgaggta cttcacgatg gacaaaaggg aagtaaaaag cgcaccgctt
     7501 attaccccgc cgaggatcag gaggatgatc gagttgccct tgtaaaggcg ggcaacgccg
     7561 accgccgccg ctacggccag aaaaccgaag agaaaacagc ttgcctgcac gaagaaccag
     7621 ctcttcgaga gcaccatccc gacggcagcg ccgaaggacg ctcccgcgag cacgcccagg
     7681 atgcccggtg acaagagggt tgatgaacat cgcttggaag gcggcccccg atatggacag
     7741 cgatgctccc acgaagagcg ccgccatgat gcggggcagg cggatattga ggaggatgtt
     7801 ctccaggagc tgcctgtcga cggacaggtt cacgccggca ttgaatatct tccagagaaa
     7861 gaacccgcag acgtcttcga tgctcaccgg gtactttccc agcaccagcg agatgagtac
     7921 ggcaatgacg agggcacagg cgagtcccat tatg
//
LOCUS       DAOPOA010000017         7943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_10193_length_7943_cov_3.859026, whole genome shotgun sequence.
ACCESSION   DAOPOA010000017 DAOPOA010000000
VERSION     DAOPOA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10193_length_7943_cov_3.859026"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(410..883)
                     /locus_tag="PKJ17_00680"
     CDS             complement(410..883)
                     /locus_tag="PKJ17_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOD74507.1"
                     /translation="MPVRKLKEFLDGNNVKYVTIAHSQAFTSQEIAASAHIPGKELAK
                     TVMIKVDGKMAMVVVPASHKVDFGLLKGVTGGQKVELATEDEFRYKFPECEIGAMPPF
                     GNLYGMDVFAAEELAEDETIAFNAGSHTELIKLAYSDFYRLVSPKIGRFTSKGNR"
     gene            complement(1007..1816)
                     /locus_tag="PKJ17_00685"
     CDS             complement(1007..1816)
                     /locus_tag="PKJ17_00685"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOD74508.1"
                     /translation="METGEDFLLKGYSLPMSPRGRAAIIDPPPWHYGGDVLHLAFRAD
                     AKKMRELLPPPLMPGPDPQEGVLWFTEWISASDANPDLSFVNPERSQYKECIVMVSCQ
                     FRGVTGYYVPYIWVDNDFTLVRGLFQGFPKKLGDVYITRLHDLTPKVGGKKAGAKVKG
                     ICVSGAQRIVEGSMEFERQAQPSDVPPVKFYLMRHFPGIEDITKPDIHEIASSRVADP
                     KVADVWVGKGEAKFHASEFEEVADLGAVTVTKAFYYSLGITITGGEVLHRF"
     gene            2000..2314
                     /locus_tag="PKJ17_00690"
     CDS             2000..2314
                     /locus_tag="PKJ17_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD74509.1"
                     /translation="MDEKTCPVTTTLGIIGGRWTLLILRDLFSGTRRFGELRKSLSGI
                     SPRTLSARLKFLEQEGIVQRKVYAEVPPKVEYSLTTRGKTLGRIIEGLKDWGREHSGG
                     KG"
     gene            2445..2978
                     /locus_tag="PKJ17_00695"
     CDS             2445..2978
                     /locus_tag="PKJ17_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74510.1"
                     /translation="MKLFRTGTAAAGQWPHICRAACPVLMALLCLLMFACETAAQRKP
                     APAAQPQPSGELSKLKQEKNDIRTARLKLFQEQSTFHGEYYKPLDRLRKQLDIQQADL
                     YEECSGEKYGKNPQYCLKEMGRFNDDVRYFNERLDDLKTRFASQHAQFQARNDELDRK
                     EREIDRRISSLQATQQR"
     gene            3419..4033
                     /locus_tag="PKJ17_00700"
     CDS             3419..4033
                     /locus_tag="PKJ17_00700"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOD74511.1"
                     /translation="MEAQKLYALPDLPYGYDALAPYISKELLTLHHDKHHAAYVNGAN
                     AILQRLDKAKTDNADFDTKATYKELSFHIGGHLLHYIFWENLAPAGKGGGGKPTGALA
                     ELLDREYGSFDRFKKIFSQAAMSVEGSGWATLTFCKLTNRPLIMQVEKHNVHIYPMFR
                     ILMALDVWEHAYYLDYKNDRAKFIEAYWNIVNWGAASKSLEEVL"
     gene            4273..6141
                     /gene="recQ"
                     /locus_tag="PKJ17_00705"
     CDS             4273..6141
                     /gene="recQ"
                     /locus_tag="PKJ17_00705"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOD74512.1"
                     /translation="MEAADTRMSPREVLKRYFGYTSFRTHQEEIVEHLMAGGDAFVLM
                     PTGSGKSLCYQVPALIRRGVCVVVSPLIALMQDQVDAMRELGIKAGFLNSTLDSREAR
                     AIERRAMEGGIDLLYVAPERLVTDGFMALLSRMEIALFAIDEAHCVSQWGHDFRPEYL
                     ELGVLAGKFPGVPRVTLTATADETTRREIIDKLRLAGARRFITSFDRPNIRYRVEIKE
                     KEKDQLLDFIRSEHPGQPGIVYCMTRKKTEEIARFLREKGLPALPYHAGLDRDVRLRH
                     QREFLEGDSTIMVATIAFGMGINKPDIRYVAHLNLPRTLEGYYQETGRAGRDGEPADA
                     WMVYSLADIVMLRQMLDASPGDEDFKAIQYRKMAAMLGYSETARCRRQVLLGYFGETL
                     AETCGNCDTCLGNVEVVDGTLLAQKALSCAYRTGQMFGVNHLVDVLVGKDTARIRAFG
                     HNSVSTFGIGGELSAREWRSVYRQLVAAGLMKVDMEKKGGLRLSGKCHPVLRGEEVFE
                     LRKDPSPSARTTGKRTLKGAGAETPNVEADGLAEGFAFDPDLWERLRSLRGELARSSS
                     LPAYTVFHNNTLREMVTRLPQTLEEMLAISGVGQAKLEKYGEIFLEAIKKHKEETA"
     gene            complement(6305..6907)
                     /locus_tag="PKJ17_00710"
     CDS             complement(6305..6907)
                     /locus_tag="PKJ17_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOD74513.1"
                     /translation="MDLTREQLEMVFAVLTGIGLSAACGFRIFLPLLALNLASMYGLV
                     GLAPGFEWIAGYQVTMAFGIATVLEIVAYYIPWLDNIMDSIASPVSVIAGTIATASLI
                     TDIPSSLKWTISIVAGGGIAGLLQGATVALRAKSTIFTAGLGNFLVASFELLASAIVA
                     FLSLILPIAAFILALLLITYCLILARRVYMKKKRGRGEAA"
     gene            7134..>7943
                     /locus_tag="PKJ17_00715"
     CDS             7134..>7943
                     /locus_tag="PKJ17_00715"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOD74514.1"
                     /translation="MKWFNNWSIGTKVMSISLFTIVIIMAGMILYFIPMVKDKLMGEK
                     KTALSDHTDIAYTIINSYEAKVKSGEFKVDDAKQRGIANLKSVRYSGNEYFFIMDTSG
                     KVIMHPINAQLDGKDVSNEKDPEGKYFFREMAQTAKEKGEGFVAYMWPKAGNSKPVPK
                     ITFVKLFKQWDWVIGTGIYVDDVEAETSKIQWQVIIATVVCAIVILCIAFFVSRKVKN
                     ALNDAVHVANDLAEGNLTVDVEIRSEDETGRLLRAMKNMVEKLREVVEGVSS"
BASE COUNT         1827 a         2299 c         2268 g         1549 t
ORIGIN      
        1 cattcttctt atcatggacg gcctgtgata ttccatgggg agtaacatac aaggtccaat
       61 gttctgaacc ctggaaccgt ctgtattgta taccggccat ccggacccaa gtcaaggtat
      121 ggtcccgaac ccttgtaaga cacaccccgt tatggcatga gagagaaaaa ccaccgggag
      181 gtcccacggc tgcggccctc catattttcg ggcccggagg cccgtacaca cccatgaagg
      241 tatcactttc ggtcgtgacg ggcaaggagg cccgcgacat ctggcctcag aggacggggg
      301 atgaggcccg ggcgttcatt gaattcctga agtccgacag gggccatcaa atattcagga
      361 gtggggttgg gaatagaccc tccgccacat ccacgatggc cctgttggct tacctgtttc
      421 cctttgaggt gaaccgtccg atcttcggac tgaccagccg atagaaatcg ctgtaggcga
      481 gctttatgag ctccgtgtga gagcccgcgt tgaaggcgat ggtctcgtcc tccgcaagct
      541 cctcggccgc gaagacatcc atcccgtaga gatttccgaa agggggcatg gcgcctatct
      601 cgcactcggg gaacttgtac ctgaactcgt cctccgtcgc cagttcgacc ttctgccccc
      661 ccgtcacccc cttgagaaga ccgaaatcca ccttatgcga cgcgggtacg acgaccatcg
      721 ccatcttccc gtccaccttg atcataaccg tcttggccag ttcctttccc gggatgtgcg
      781 ctgaggcggc tatctcctgg gacgtaaagg cctgagaatg agcgatggtg acatatttta
      841 cattgttccc gtcgaggaac tccttcaact ttcgcaccgg catggcccac ctcctggggg
      901 atcgtatgtg tttcccatct atatagtata cacccaaccc gccgttttac gaatcgcccc
      961 gcgggaggtg cccgacccac cagggacagg aagccgcgcc ggcgtctcag aacctgtgca
     1021 ggacctctcc acccgttatg gttatgccca gactgtaata gaaggccttc gtgaccgtga
     1081 cggccccaag gtcggccacc tcttcgaact cggaggcatg gaacttcgcc tcccctttcc
     1141 cgacccacac gtcggccacc ttcgggtctg cgacacggct cgaggctatc tcatgtatgt
     1201 cgggcttcgt gatgtcctcg atgccgggaa aatgacgcat gaggtagaac ttgaccgggg
     1261 gcacgtccga cggctgcgcc tgcctttcga actccatgga cccttccact attcgctgcg
     1321 ccccggaaac acagatgccc ttcaccttcg cgcccgcctt cttccctccc acctttggcg
     1381 tgaggtcgtg gagcctcgtg atgtacacgt ctcccagctt cttggggaaa ccctggaaga
     1441 gccctctcac cagcgtaaag tcgttgtcca cccatatgta gggaacgtag tacccggtga
     1501 ccccccggaa ctgacagctg accatcacga tgcactcttt gtactgggac ctttcggggt
     1561 tgacgaagct caggtccggg ttggcatcgc ttgcggagat ccattccgtg aaccacagga
     1621 cgccttcctg cgggtccggt cccggcatga gcggcggggg caggagttcg cgcatcttct
     1681 ttgcgtccgc cctgaacgca aggtgcaaga cgtctccccc gtaatgccac ggcggagggt
     1741 caattatcgc ggcccttccc cgcgggctca tcggaagcga gtatcctttc agcaaaaagt
     1801 cttcacctgt ctccataaca aacctccctc tcgttatctc atccgtctac tgtccgtgcc
     1861 gcgccaacgt ccgccccacc aatcacccat cgcctggcaa cccgcctgtc acctgacaca
     1921 ctatcacacc acagtttact ggaacaagta gttacaatta tgatatatag ttatacttgg
     1981 tgaatcattg gggaaacaca tggacgaaaa gacgtgtccc gtcacaacca ctcttggcat
     2041 catcggcggg cgctggacgc tcctgatact cagagacctg ttctcaggca cccgtcgctt
     2101 cggcgagctc cgcaagtcct tgagcggcat cagtccccgc accttgtcgg cgcgtctcaa
     2161 atttctcgag caggaaggca ttgtgcaaag aaaggtctac gcggaggtcc ccccaaaggt
     2221 cgagtactcc ctgaccaccc gcggcaagac cctcggcagg atcatagagg gcctgaagga
     2281 ctgggggagg gagcatagcg gcgggaaggg gtgaatgcgc cgtcacgacc cacgggtctt
     2341 gtagcccatg ggtcctatta tcttcttgca atagacccgc gggctatagg aaaatacagg
     2401 aggatgattt cttccggccg gaacacaatc gaggaggcag acatatgaaa ctattccgaa
     2461 caggcacggc tgcagcaggg cagtggccgc acatctgccg cgcggcatgt cctgtgctta
     2521 tggcgcttct atgcctcctc atgttcgcgt gcgaaacggc ggctcaacgc aagccggctc
     2581 cagccgctca accgcagccg tcgggagagc tgtccaaact caaacaggag aagaacgaca
     2641 tcaggaccgc ccgtctcaag ctcttccagg aacagagcac ctttcacggt gagtattaca
     2701 aacccctgga ccggctgaga aagcagctcg acatacagca ggcggacctt tacgaggaat
     2761 gctcgggaga gaagtatggc aagaacccgc agtactgcct gaaggagatg ggcaggttca
     2821 acgacgatgt cagatacttc aatgaacgcc tcgatgacct gaagaccagg tttgccagcc
     2881 agcacgcgca gttccaggcc aggaacgacg agctcgacag gaaagaaagg gaaatagacc
     2941 gcaggataag ctccctccag gccacacagc agcggtagaa agaaggcttg aacagcttga
     3001 atcgcttgag ccgcttgagc gttccttgtc cttgtcatcc cagcggaagg accgttgcat
     3061 gaacgggaaa acagactctc acggtggacg gcagggtgtt gcaggaacag tcaagtgtca
     3121 ttcctgtgtt catttctata ttacctatga caagtcgttc ccttacgggt gcagggctat
     3181 ggggttcaag tcccgggtca taccgtcgag agaggtgttc tcaagctccg gaatggagtg
     3241 ccagattttt tcaagaaaag aggggagttc cttgtagggc aaggccgtac gcggggagga
     3301 gatcatcatc gttcggctcc cggcaaatta cgtggtacaa ttgatatatg cggccggctt
     3361 cactcgagtc aggaacagac gggtaaaccc gcaacaagaa ttcaaaggag gtaacgaaat
     3421 ggaagcacag aaactctacg ctttacccga tctcccctat ggttatgacg cgcttgcgcc
     3481 atacatatcc aaagaacttc tcacgctgca tcacgacaag caccacgcgg cctacgtaaa
     3541 cggggccaat gccatcctgc agagactcga caaggcgaaa accgacaacg cggactttga
     3601 cacgaaagcg acctataaag aactgtcctt ccacatcggg gggcatctcc tccattatat
     3661 cttctgggag aacctggcac ccgctggcaa gggcggcgga ggcaaaccga cgggggccct
     3721 cgccgaactc ctcgacaggg aatacggctc tttcgaccgt tttaagaaga tcttttccca
     3781 ggcggccatg agcgtggagg gctcgggctg ggcaacgctc accttctgca aactgacgaa
     3841 cagaccgctg atcatgcagg tggagaaaca caacgttcac atctatccca tgttcaggat
     3901 cctcatggcc ctcgatgtct gggaacatgc ctactacctg gactacaaga acgacagggc
     3961 gaagttcatc gaggcatact ggaacatcgt caactgggga gccgcgagca agagcctgga
     4021 ggaagtgctg tgaagacggc ttgagccgct tgaacgttgt tcgtcattcc ggctttgggc
     4081 cggagtccag gagggatgtg gaaacgcaga aagcctgggt ttcggtcttg agccggttga
     4141 tgtgtttcgt gattattatt cctgtcacct cgtttttttc tcctctaaaa agtaatatat
     4201 ctccggtgtg gttttaagcg gcccgttggg gtaatctagt ggcgggcata tttcgtcgag
     4261 cgagggttgt ctatggaagc cgcagataca cgcatgtcgc cgcgggaggt tctgaagaga
     4321 tatttcggat acacgtcctt tcgcactcac caggaagaga tcgtcgagca tctcatggcc
     4381 gggggggatg ccttcgtgct catgcccaca ggcagtggaa aatcattgtg ctaccaggtc
     4441 ccggccctga tccggcgggg tgtgtgcgtc gtggtctccc ccctcatagc gctcatgcag
     4501 gatcaggtgg acgctatgcg ggaactgggc atcaaggcgg gctttctcaa ctccaccctc
     4561 gactcgcgcg aggcccgcgc aattgaaagg cgcgcaatgg aaggcggtat cgatctcctc
     4621 tacgtggcac ccgagaggct tgtcaccgac ggtttcatgg cccttctcag cagaatggag
     4681 atagccctct tcgcgataga cgaggctcac tgcgtctccc agtggggaca cgatttccgg
     4741 cccgagtacc tggaactggg cgtcctggcg ggaaaattcc ccggggtgcc gcgtgtgacg
     4801 ctcaccgcga cggccgacga gaccacgaga cgggaaatca tcgacaaact gcgcctggcc
     4861 ggggcgaggc gctttatcac aagcttcgac aggcccaaca tacgttacag agtggagatc
     4921 aaggagaagg aaaaggacca gcttctcgac ttcatcagat cagagcaccc cgggcagccg
     4981 ggcatcgtct attgcatgac acggaagaag actgaagaga tcgcgcgatt ccttcgggag
     5041 aaggggcttc ccgccctgcc ctaccatgcg ggtctcgatc gggacgtgcg gctcaggcac
     5101 cagagggaat tccttgaagg ggacagcacc atcatggtcg cgaccatagc cttcggcatg
     5161 ggcatcaaca agcccgacat acgctatgtc gcccacctca accttccccg gaccctggaa
     5221 ggctattacc aggaaacagg gcgcgcggga cgtgacggag aaccggcgga cgcgtggatg
     5281 gtctacagcc tggccgacat cgtcatgctc cggcagatgc tcgacgcctc cccgggagat
     5341 gaagacttca aggcaatcca gtaccgcaag atggcagcga tgctcggcta ctccgagacc
     5401 gctcgctgcc gacggcaggt gctcctcggg tactttggag agacccttgc ggaaacctgc
     5461 ggcaactgcg acacatgcct cgggaatgtg gaggtcgtgg acggcaccct gctcgcgcag
     5521 aaggcactct cctgcgccta caggacaggt cagatgttcg gggtcaacca tctcgtcgac
     5581 gtactagtcg gaaaggacac ggcacgcata cgggcctttg gccacaacag cgtgtcgaca
     5641 ttcggcatag gcggggagct gtcggcacgt gaatggaggt ccgtgtacag gcagctcgtc
     5701 gccgcggggc tcatgaaggt ggacatggaa aagaaggggg ggctgcgtct gtcgggcaaa
     5761 tgccaccccg tgctgcgagg agaagaggtg ttcgagctcc gcaaggatcc ctcaccttcc
     5821 gcgaggacca cagggaagag gaccctcaag ggcgccgggg cagagacccc aaacgtcgaa
     5881 gctgatggcc ttgcggaggg cttcgccttt gaccccgacc tctgggaacg tctccgctca
     5941 ctgcgaggag aacttgccag aagctcctct ctccccgcgt acaccgtatt tcacaacaac
     6001 accctcagag agatggtgac acgcctgccg cagacactgg aggagatgct ggccatctca
     6061 ggagtcggcc aggcaaaact ggaaaagtac ggggagatct ttctcgaagc cataaagaaa
     6121 cataaggaag agacagcttg aaccgcttga acagcttgag ccgcttgaac ggggaaagca
     6181 aaggacaatg acactgcccg gagcccgggg tgacaactgg gagccggacc gtgctctttt
     6241 gtctttttct tttaacgttc aagcggttca agcggttgca acaatctttt tcggctcaag
     6301 ctgttcaagc cgcctctccc ctgcccctct tcttcttcat atacaccctc cgggccagga
     6361 tgaggcagta ggtgatgagg agcagagcga ggatgaaggc ggcgatgggg aggatgagcg
     6421 acaggaaggc gacgatggcg gacgcaagga gctcgaagct ggcgaccagg aaattgccca
     6481 gcccggcggt gaagatggtg gatttggctc gcagggccac cgtggcgccc tgaagcaggc
     6541 ccgctatccc gcccccggca acgatcgaaa tggtccactt cagcgaagag gggatgtccg
     6601 ttatgagcga tgccgtcgcg atcgtgcccg cgatgacgct caccggcgag gcgatggagt
     6661 ccatgatgtt gtcaagccag ggaatgtaat aggcaacgat ctcgagcacc gtggcaatcc
     6721 cgaaggccat cgtcacctgg taccccgcga tccattcgaa tcccggcgcc aggccgacga
     6781 ggccatacat ggaggcaagg ttcagggcga gaaggggcag aaagatcctg aacccgcagg
     6841 ccgcgctgag gccgatcccc gtaaggaccg cgaataccat ttcaagctgt tcccgcgtca
     6901 gatccatacc ttaattttcg tccttcccgg ataccagggt caagaagaga atgcttccgt
     6961 gtaggactcg tcttacaaca aaataagaca ataggccata ttcccccgca tcaaaatacc
     7021 gacaactgat gatatgttgg agagttcacc ttcacagcag aatagccggc aggctcaggt
     7081 aatccatcac ggtaaagagc agcacagtcc tgcatccata caaggaggtt attatgaaat
     7141 ggtttaacaa ctggtccatc ggaacaaaag tgatgagcat atccctcttc accatcgtca
     7201 tcatcatggc cgggatgatc ctctacttca tccccatggt aaaggacaag ctgatgggcg
     7261 agaagaagac ggcattgagc gatcacaccg atatcgccta cacgatcatc aattcttacg
     7321 aggcgaaggt caagtccggc gagttcaagg tcgacgatgc aaaacagcgg ggtatcgcaa
     7381 acctgaagtc cgtccgctac tccgggaacg agtacttctt catcatggac acgagcggca
     7441 aggtaatcat gcatcctatc aacgcccagc tcgacgggaa ggacgtgtcg aacgagaagg
     7501 acccggaggg caagtacttc ttccgcgaga tggcccagac cgccaaggag aagggggaag
     7561 gcttcgtggc ttacatgtgg cccaaagcag gcaactccaa acccgttccc aagatcacct
     7621 tcgtgaagct tttcaaacag tgggactggg tcatcggcac gggtatctat gtcgatgacg
     7681 tcgaagcgga gacctccaag atccagtggc aggtcatcat agccaccgtc gtctgtgcca
     7741 tcgtcatcct ctgcatcgcc ttcttcgtct cccgcaaggt gaagaacgcg ttgaacgacg
     7801 ccgtccatgt ggccaacgac ctggccgagg gcaacctcac cgtcgacgtg gagatacgga
     7861 gcgaggacga gacgggcagg ctccttcggg ccatgaagaa catggtggag aagctccgcg
     7921 aggtcgtgga gggtgtcagc tcc
//
LOCUS       DAOPOA010000018         7778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_10466_length_7778_cov_6.029004, whole genome shotgun sequence.
ACCESSION   DAOPOA010000018 DAOPOA010000000
VERSION     DAOPOA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10466_length_7778_cov_6.029004"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..847)
                     /locus_tag="PKJ17_00720"
     CDS             complement(56..847)
                     /locus_tag="PKJ17_00720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD74515.1"
                     /translation="MERQQDARPPLSVYMITLNNSATIARALESVAGWADEIIVVDSH
                     SSDGTADIASKYTEQVLQFDTTSQPEKYQYAQDRCRNRWVLFIDADEWLTEDFRKETE
                     TILASGSASDGYIADRRNIYLGREIKHGGWYPDREIRLYRKDRGGWEGGIHAKVHVKG
                     EVGHMRNHYLHTPYADSAHQIRTIDRYSEAYARDLLAAGRRFHMANMLLRPIWRFFRD
                     YIIKMGFLDGVPGIIIMASTMYYVFMKHARLWEMERDDGGKKGVR"
     gene            complement(980..1462)
                     /locus_tag="PKJ17_00725"
     CDS             complement(980..1462)
                     /locus_tag="PKJ17_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOD74516.1"
                     /translation="MGKIVNGLDELKAIIEREKAAGKRVVFGNGCFDIVHVGHVRYLR
                     GAKELGDVLVVAVNDDASVTGLGKRKEVVTPAAERAEIISAIDCVDYVILFADPTVEN
                     LLRELKPHIHAKGTDYTEGNVPERDIVMSYGGRVAIVGDPKDHSTRDIIKTIKNLREK
                     "
     gene            complement(1530..2576)
                     /locus_tag="PKJ17_00730"
     CDS             complement(1530..2576)
                     /locus_tag="PKJ17_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOD74517.1"
                     /translation="MKRESEDKLRKPGRKAKERDGAGLIRFLDRFRGKKICVVGDIIA
                     DVFILGKPYRLSREAPVVVVKYEESKIYPGSAGNTINNLAALGATVYPVGFVGSDSMG
                     GKLMDFFSTRKTINTAGIIRVSGDTVTKTRILAGDTHVSKQQVIRIDKDDGRRRTTKE
                     KALLMERIAGICPHVDAVIVSDYGHGAVSGKVIEYMKILANDKIVVGDSRYGLKKYSG
                     FTMITPNESEAFALSGLRENEPIEDAGGKVLSGMRLSALLVTRGNKGMTLFLKDGKVH
                     HIPISGKDDVTDVTGAGDTVCAAVALSLASGAGFLDASRIANYSAGIVVMKRGTATVT
                     VAELKRSIENGGKI"
     gene            complement(2560..3555)
                     /gene="waaF"
                     /locus_tag="PKJ17_00735"
     CDS             complement(2560..3555)
                     /gene="waaF"
                     /locus_tag="PKJ17_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOD74518.1"
                     /translation="MTGKTIVYLPNWLGDMVMAVPFLHALRQHSQDEVWAMGKESAIH
                     LYNGLGLFDRFVPISGKGLGLFFETTNRLNRVGFTRAIALPHSFRSTLVFFNLRVRDV
                     IGYARNRRGFMLNVKVPEARELQSTVEHYLRIADTLGVPRTIEAPVLSVSADEEKKFD
                     EEFSDMTRPYGVMIVGAQYGPSKCWPAGYFSEFADEIIERYGMNVYMLPGRGEDVLAR
                     KIHEGIRAGERARIRSMNIRDMKVCLSRAAFVVSNDTGPRHISAALSVPTVVLAGPMD
                     ERYTAYPGGHTRVVSKDMACRPCNKKKCDGNHECMTGIKPRDIAEIVGDILEKRV"
     gene            complement(3524..5026)
                     /locus_tag="PKJ17_00740"
     CDS             complement(3524..5026)
                     /locus_tag="PKJ17_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD74519.1"
                     /translation="MRFLFVYPNINTQVGFNYGISYISGFLRSRGIETGLLNINDKLG
                     YPMDMDRIRHDVLAFKPDIIGFSVLTNQHKYALDIARDIKTYLDVPILFGGIHVTMDP
                     METLMRDEVDLICVGEGEEAVAELVEKGDPRGIANIGWKEKGRPVIEPLRPYKDIASL
                     PFKDYEIFDFQRLIDAKDGWVGLTASRGCPFRCTYCLNHKIIEVYKESGHRPRGYIRR
                     HTVDQMISEIEYLLGRYTGIKMFIFDDDIFTFDKAWLREFTERYRAVTDISFVCNAHA
                     RIFDEETAELLKGAGCRIVKFGLESGSDRIRRKVLSRYMTNKHIEDAFAVAHRHGLHT
                     SAFVMLGLPHETIGDIKQTIDLIAAIKPGRFRWSLFFPFVGTKAYNIALSSGQVDLTE
                     MERLDNFTDETCMRLGPDVDLYCDKVKSALCAFVNAHSGLEHTEGFRRLAEEIENMDE
                     KTWREKKASILDRVEELNQEMEKSGGLHYTVRYNPFMGVRSDWKDDSISA"
     gene            complement(5031..6092)
                     /locus_tag="PKJ17_00745"
     CDS             complement(5031..6092)
                     /locus_tag="PKJ17_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOD74520.1"
                     /translation="MAQPDSILVIRLSSLGDVLMSVPAVRALRRRFPGAHISWLAEGS
                     VTGILAHQDFIDEVIFFPRAALTKHLRSGHLATEAYILRDFLKDLRKREYDCILDLHG
                     IAKSVALMKLARGKRRIGFGKTFAKDMSHLFYGERVEGTEKRIHKVERNMLAARYLGC
                     ADPPGNVPLLVHDSARTYIDDFLRSRDVASPVIAVNPFASRDSAFKRWPLERYADLIG
                     MIRRERMGSVVIIWGPGEREEAQRLAAMVGDGARLACQTDIAQLYALLAQSAVYIGGD
                     TGTTHLASAAGVPVLCLFGPTDFIVNRPYSKNSVIIRKDVSCSPCKKKDCRTRECLMT
                     ISPDEVFAALRKMPFRKEA"
     gene            complement(6076..6516)
                     /locus_tag="PKJ17_00750"
     CDS             complement(6076..6516)
                     /locus_tag="PKJ17_00750"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOD74521.1"
                     /translation="MPGQANDHARDDGGFIDVPVRVRYADTDRMGIVYYGNYPAFFEM
                     GRSEYMRQKGYPYRDLETLGYHFVVVGLEAKYYNSATYDDILIVKTRVTELKSRGITF
                     HYVITRDGTLVVEGSTKHICVNTDKKPVTIPQKLIEIFRDGTAG"
     gene            complement(6519..7394)
                     /locus_tag="PKJ17_00755"
     CDS             complement(6519..7394)
                     /locus_tag="PKJ17_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD74522.1"
                     /translation="MKYEGTIYRPPSEADSLILQVTIGCSHNKCTFCGSFKEKKFRVR
                     SFEEIAEDVNEAKQYARYIRKVFLADGDALIIPQKRLLPIVQLVRDAFPKMERIGIYG
                     NTKSILKKSVEELKALKELGVGIIYLGVESGDQVVLDRVCKGTTLDKTAEAAKRVKDA
                     GILLSVTVLLGLGGVERSRAHAEETGKFLSRIQPDYAGALSVIVVPGTVLADQIKTGE
                     FVVPDPYMLLEELYTMIQNTNVEHTYFASNHASNYLPVKAWLPEEKEKTLRAIQHVLA
                     ERDPSMLRPEFMRAL"
     gene            complement(7391..7669)
                     /locus_tag="PKJ17_00760"
     CDS             complement(7391..7669)
                     /locus_tag="PKJ17_00760"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOD74523.1"
                     /translation="MAENPVKRYGFIVFLSTIFFHLILMDGGIYDLLKVKLKTRTAKL
                     AIMQMEDENAALSRELRRIREDDQYLEEMVRKKYGLVREGEKVYRVEQ"
BASE COUNT         1425 a         2272 c         2223 g         1858 t
ORIGIN      
        1 tatgcagagg ttgctgccga tgaagccgag ccctccggtg acaaggacgt ctttgttatc
       61 gaacaccttt ctttcctcca tcgtctctct ccatctccca caatcgggcg tgcttcatga
      121 agacgtagta catggtggac gccatgatga tgatgccggg cacgccgtcg aggaaaccca
      181 tcttaatgat gtaatccctg aagaagcgcc agatgggacg gagcagcata tttgccatgt
      241 gaaagcggcg ccccgccgcg agaaggtcac gggcgtaggc ctcggaatag cggtcgatgg
      301 tccggatctg atgggccgaa tccgcgtaag gcgtgtggag gtagtggttc ctcatgtgcc
      361 ccacctctcc cttcacgtga accttcgcgt gtatgccgcc ctcccagccg ccccggtctt
      421 tcctgtagag ccgtatctcc cggtccgggt accagcctcc atgttttatc tcccgcccca
      481 ggtagatgtt gcgccggtcg gctatgtagc cgtcgcttgc cgaacccgag gcgagtatgg
      541 tctccgtctc tttcctgaag tcttccgtga gccactcgtc ggcatcgatg aagaggaccc
      601 agcggttcct gcagcggtcc tgggcgtact gatacttttc cggttgactt gtggtatcga
      661 actgcaggac ctgttccgtg tattttgagg cgatgtcggc agtgccgtcg gagctgtggg
      721 aatcgacgac tatgatctca tccgcccatc cggcaacgct ttcgagcgcc cgtgcgatgg
      781 tggcgctgtt gttcaaggtg atcatgtaca cggatagggg tggtctcgcg tcctgttgtc
      841 tttccattct catgtggtcc gatggtgtaa caatacacta aaaagtgttt gaaatcaaat
      901 gagaaggaag gggcagggga tagataggtc ctatctttcc tgtaggtcct gttgggttgt
      961 tctatccgga cccttcccgc tatttttctc tcaggttctt tatcgttttt atgatatccc
     1021 gcgtcgaatg gtccttgggg tcgccgacta tggcgaccct tccgccgtag gacatgacga
     1081 tatcgcgctc ggggacattt ccttcggtgt aatcggttcc cttggcgtgg atgtggggtt
     1141 tgagttccct gaggaggttt tccacggtgg ggtcggcgaa gaggatgacg taatcgacgc
     1201 agtcgatggc tgatatgatc tccgcccgct cggccgccgg cgtgacgacc tccttgcgct
     1261 tgccgagacc ggtgacggag gcgtcgtcat tgacggcgac aacgaggacg tccccgagtt
     1321 ccttcgcccc cctgaggtat cgcacatgcc ctacatggac gatatcaaag cacccgttcc
     1381 cgaacacaac cctcttcccc gccgccttct ccctctcgat gattgcttta agctcatcca
     1441 ggccattgac tatcttcccc attgaacgcc catcccattt tggcccctcg aactcttgaa
     1501 ctcttgaacc cttgaacccg gaaccgtttt caaatctttc ccccgttctc tatcgacctt
     1561 ttcagctctg ccacggtaac cgtcgccgtc ccccgtttca tgacaacgat ccccgcggag
     1621 tagtttgcga tccgcgaggc atcgaggaag cccgccccgc tcgccaggga gagagcgacg
     1681 gccgcgcaga ccgtgtcgcc ggccccggtc acgtccgtca cgtcatcctt gcccgatatg
     1741 gggatgtgat ggacctttcc gtccttgaga aagagcgtca tgcccttgtt gccgcgggtg
     1801 acaagaaggg cggaaaggcg catacccgaa aggaccttcc ctcccgcgtc ctctatcggt
     1861 tcgttctccc tgaggcctga gagggcaaat gcctcggact cgttgggggt gatcatggtg
     1921 aaccccgaat acttcttcag gccgtaacgt gaatcgccga cgacgatctt atcgttggcg
     1981 aggatcttca tgtattcgat caccttgccg ctgacggcgc cgtgcccgta gtcggagacg
     2041 atcaccgcgt ccacgtgggg gcagatcccg gcgatccttt ccatgaggag ggccttctcc
     2101 ttcgtggtgc gcctgcggcc gtcatccttg tctatgcgga tgacctgctg tttcgagacg
     2161 tgggtgtccc cggcgaggat ccgtgtcttt gtcaccgtgt caccgctgac gcggatgatt
     2221 cccgccgtgt tgatggtctt cctcgtggaa aagaaatcca tgagcttacc gcccatcgaa
     2281 tcgcttccca cgaagccgac gggatagacc gtcgccccga gcgcggcaag gttgttgatg
     2341 gtgttgccgg cgctcccggg atagatcttc gactcctcgt acttgacgac gaccacgggg
     2401 gcctccctgg agaggcggta gggcttcccc aggatgaaca cgtcggctat gatgtcgccc
     2461 acgacacata tcttcttgcc gcgaaaacgg tcgagaaagc gaataagccc cgccccgtcc
     2521 cgttctttcg ccttccgccc cggtttcctc agcttgtcct cagactctct tttcaaggat
     2581 atcccccact atctcagcga tgtcccgcgg ctttattcct gtcatgcatt catggttccc
     2641 gtcgcatttt ttcttgttgc aggggcggca ggccatgtcc ttcgagacca cgcgggtgtg
     2701 tccgcccgga taggccgtat aacgttcatc catgggcccg gcaagaacaa cggtcgggac
     2761 ggacagcgcc gcggagatgt ggcgcgggcc ggtgtcgttg ctgacgacga aggccgcccg
     2821 tgacaggcac accttcatgt cccgtatgtt catggatctg atccgggcgc gctccccggc
     2881 gcgtatccct tcgtggatct tccgggccag gacgtcctcg cccctgccgg gaagcatgta
     2941 gacgttcata ccgtaccttt cgatgatctc atcggcgaac tcggaaaaat acccggcagg
     3001 ccagcatttc gacgggccgt actgggcgcc cactatcatg acgccgtaag gcctcgtcat
     3061 gtcggagaac tcctcgtcga acttcttctc ctcgtcggcc gacacggaaa ggacgggtgc
     3121 ctcgatcgtc cggggtactc cgagcgtgtc cgcgatcctg aggtagtgtt cgactgtgga
     3181 ctgaagctcc cttgcctcgg ggaccttcac gttcagcatg aagccccgcc tgttccgtgc
     3241 gtatcctatg acgtcgcgaa ccctcaggtt gaagaagacg agggtcgacc tgaaggaatg
     3301 gggcagggcg atggcccggg tgaagcccac cctgttgagc ctgttcgtcg tttcgaagaa
     3361 gaggccgagc cccttcccgc tgatgggaac gaaacggtcg aagagtccga gtccgttgta
     3421 caggtggatg gcgctttcct tccccatggc ccatacctcg tcctgtgagt gctgccggag
     3481 ggcatggagg aagggaacgg ccatcaccat gtcgcccagc cagttaggca gatatactat
     3541 cgtctttcca gtcacttcgc acgcccataa aaggattgta tctcacagta taatggagtc
     3601 cgcccgattt ttccatttcc tggttgagct cttccaccct gtcgaggata ctcgcctttt
     3661 tttcgcgcca ggtcttctcg tccatgttct cgatctcttc cgcgaggcgc cggaagcctt
     3721 cggtgtgttc cagccctgag tgagcgttca cgaaggcgca gagcgcgctc ttgaccttgt
     3781 cgcagtaaag gtccacgtcg gggccaagcc tcatgcaggt ctcgtcggtg aagttgtcga
     3841 gcctttccat ctccgtgagg tcgacctgtc ccgaggaaag ggcgatgtta tacgcctttg
     3901 ttcccacgaa ggggaagaag agggaccagc ggaaacgtcc cggcttgatg gcggcgatca
     3961 gatcgatggt ttgtttgata tcccctatcg tctcgtgggg aagaccgagc atcacgaagg
     4021 ccgacgtgtg gagcccgtgt ctgtgcgcga cggcgaaggc gtcctcgatg tgtttgttcg
     4081 tcatatagcg gctcaggacc tttcggcgta tcctgtcgct gccgctttcg agcccgaact
     4141 tgacgatgcg gcatcccgcg cccttcagga gctcggccgt ttcctcgtcg aaaatgcggg
     4201 cgtgagcgtt gcagacgaag cttatgtccg tgaccgccct gtaccgctcg gtgaactccc
     4261 tgagccaggc cttgtcgaag gtgaagatgt cgtcgtcgaa aatgaacatc tttatccctg
     4321 tatagcggcc gaggaggtac tcgatctcgg agatcatctg gtccacggtg tgtcttctga
     4381 tgtaaccccg cgggcgatga ccactttcct tgtagacctc tatgatcttg tggttgaggc
     4441 aataggtaca tcggaaggga cagccccgtg acgcggtgag tcccacccat ccgtccttcg
     4501 cgtctatcag tctctggaaa tcgaagatct cgtagtcctt gaagggcaga ctcgctatgt
     4561 ccttgtaggg ccgaaggggt tcgatgacgg gccttccttt ctccttccag ccgatgttcg
     4621 caatgccccg gggatcgcct ttctcgacaa gctcggcaac ggcctcttct ccctcgccga
     4681 cgcatatgag gtcgacctca tcgcgcatca gcgtttccat gggatccatc gtgacgtgga
     4741 tgccgccgaa aaggatgggc acgtcgaggt acgtcttgat atcgcgggct atatcgaggg
     4801 cgtacttgtg ctggttggtg aggacggaga agcctatgat gtcgggcttg aaagcgagga
     4861 cgtcatgcct gatcctatcc atgtccatgg gatagcccag cttatcgttg atgttgagga
     4921 gccccgtctc gatgcccctc gacctgagga accccgagat ataggagatg ccgtaattga
     4981 accccacctg ggtgttgatg ttgggataga cgaagagaaa gcgcatacct ttaagcctct
     5041 ttcctgaaag gcatcttcct cagtgccgcg aagacctcgt ctggagatat ggtcatgagg
     5101 cattcccggg tcctgcaatc cttctttttg caggggctgc aggagacatc cttccgtatt
     5161 atgacgctgt ttttcgagta agggcggttc acgatgaagt cggtggggcc gaagaggcac
     5221 aggacgggga cgcccgcggc ggacgcgaga tgggtcgttc ccgtatcgcc ccctatgtaa
     5281 acggcgctct gcgcgagaag agcgtagagc tgtgcgatgt ccgtctggca ggcaagccgg
     5341 gccccgtccc ccaccatcgc ggcaagcctt tgggcctctt cccgttcgcc cggtccccag
     5401 attattacga cgctgcccat gcgttcccgc cgtatcatcc cgatgaggtc ggcgtaacgt
     5461 tcgagaggcc atcttttgaa ggcggaatct ctgctggcaa aggggttcac ggctatcacg
     5521 ggggacgcga cgtcgcgtga tctcaaaaaa tcgtctatgt atgtccgggc gctgtcgtgc
     5581 acgagaaggg ggacattccc gggagggtcg gcgcatccga gataacgggc ggcaagcatg
     5641 ttacgttcca ccttgtgtat cctcttttca gttccctcga ctctttcgcc gtagaaaagg
     5701 tggctcatgt cctttgcgaa tgtctttccg aaccctatcc gtctttttcc ccgcgccagc
     5761 ttcataaggg ccacgctttt cgcaatgccg tggaggtcga ggatgcagtc gtattcccgc
     5821 tttctaaggt ccttcaggaa atcacggaga atataagcct ccgtggcgag atgacccgat
     5881 ctgaggtgtt tcgtgagggc cgcccggggg aagaatatta cctcgtcaat gaaatcctgg
     5941 tgcgcgagga ttcccgtcac ggaaccttcg gcgagccatg agatgtgcgc cccgggaaag
     6001 cgccgcctca aggcccttac cgctggcacg ctcatcagga catcaccgag ggagctcagt
     6061 cggatgacga ggatgctatc cggctgtgcc atctcggaag atttctatga gtttctgtgg
     6121 tatcgtgacg ggcttcttgt cggtattcac acagatatgc tttgtcgatc cctcgacgac
     6181 cagggtcccg tccctcgtta tcacgtagtg gaaggtgatg ccccgcgact tgagttccgt
     6241 cacgcgggtc ttcacgatga ggatatcgtc gtaggttgcc gaattgtagt atttggcctc
     6301 gagaccgacc acgacgaagt ggtatcccag ggtctccagg tccctgtagg ggtagccttt
     6361 ttgccgcatg tactcgctgc gtcccatctc gaagaaggcc gggtaattcc cgtagtaaac
     6421 gatgcccatc ctgtcggtgt cggcgtagcg gacgcgcacc ggcacatcga tgaaccctcc
     6481 gtcgtccctt gcgtggtcat tcgcctgtcc cggcatgatc acagggccct catgaactcg
     6541 ggtctgagca tcgaaggatc gcgctcagcg aggacgtgtt gtatggccct gagtgtcttc
     6601 tctttctcct cggggagcca ggccttaacg gggaggtagt tcgacgcgtg gttcgacgcg
     6661 aagtaggtgt gttccacgtt ggtgttctgg atcattgtgt agagctcttc gagaagcata
     6721 tagggatcgg gcacgacgaa ttcacccgtc tttatctggt cggcgagcac cgtgccgggc
     6781 acgacgatga cgctcaaggc ccccgcgtag tcgggctgga tcctgcttaa gaactttccc
     6841 gtctcttccg cgtgtgccct gctccgctcc accccgccga gccccaaaag gacggtcacg
     6901 gaaaggagta tgcccgcgtc cttcaccctc ttcgcggcct cggcggtctt gtcgagggtt
     6961 gttcccttgc agaccctgtc gaggaccacc tggtctcccg actccacgcc gaggtagatg
     7021 atgccgacgc cgagttcctt gagggccttc agctcttcca cggatttctt caggatcgat
     7081 ttcgtgttcc cgtagatgcc tatgcgttcc atcttcggaa aggcatccct gacgagctgc
     7141 acgatgggca agagcctttt ctgggggatg atcaacgcgt cgccgtcggc gaggaagacc
     7201 ttcctgatat agcgcgcgta ctgctttgcc tcgttcacgt cctcggcgat ctcctcgaag
     7261 gagcgtaccc ggaatttctt ctctttaaag gagccgcaga aagtgcattt gttatgggag
     7321 cagccgatgg tgacctggag gataagactg tccgcctcgc ttggcggcct gtagatcgtt
     7381 ccttcgtatt tcattgttca accctgtaca ccttttcccc ttctcgcaca agaccatatt
     7441 tcttccttac catctcttcg aggtactggt cgtcctcgcg tatcctgcga agctcacgcg
     7501 agagcgccgc gttctcatct tccatctgca tgatcgccag tttggctgtc ctcgttttca
     7561 gtttgacctt caaaaggtcg tatattccac cgtccatgag gataaggtgg aagaaaatgg
     7621 ttgagagaaa gactataaat ccgtatctct ttacgggatt ctctgccatg ctttctttat
     7681 attaaaggca cggaagcaaa atgtcaaccc gtgagaggcg ggcgccagta gttgggcatg
     7741 gacatactta ccaccctggg gtatacccag aagaaaag
//
LOCUS       DAOPOA010000019         7685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_10622_length_7685_cov_3.735256, whole genome shotgun sequence.
ACCESSION   DAOPOA010000019 DAOPOA010000000
VERSION     DAOPOA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10622_length_7685_cov_3.735256"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2002
                     /locus_tag="PKJ17_00765"
     CDS             <1..2002
                     /locus_tag="PKJ17_00765"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74524.1"
                     /translation="ILQNVDFPMVMNTTLLCEDLSTITRDLARQRLMANIFSGKRTAA
                     AVENKEKITAIDDLLQDRARHGWKLVKCFNSYLVWDKDIEKLQEKIQAVRVALAKAVD
                     GAGVFAEWMRKEGAFIASLPGCGVRSYDPHIVFGHDVLKLVPLRGMYRGDRSEPAIVL
                     RNRYGGVTALNPFSSRQNRWAGLVIGPTGSGKSVLTNGIIAGAMAYDPVVVVIDMSKA
                     SSYEPVVSNFGGSFIPITFGSNDNRVNMFDLRVGFEQPAGSKIMSLNAIFTAMLGEEG
                     RGVAKETQSILERAVRRMYDNLFKEEPRLVRNVRIVNGTQEEIFSDAPSFDTYIEYRN
                     HYIERFLKTGERKDFLRAETAQSLATPTLNDLMRILASDPAINTNARDRQIAEEMRRI
                     LNLYAQGEQAALLNGVTNFSVDRDIFCIHMGLVSERKEMLALLLLLYRDFAYRKAVYL
                     PNEMPPFLGAASIDWILAAQRRPKLFVYDEFHNLKNNEPILDVLDKDARQQRTLGLAT
                     YLVTQDILDVAEKGNHFISAASNKYFTRHVSPDNPTMEGIDNLVRIIGLGSQERELLL
                     SLKFYPGKYAEILALNEDIGKGVLVNVQTPRTRWSYTTHKDERYLRETVASTLKEKGV
                     SQRRAQAVAVEVLSDMLPLGSIGQSVDIGAVMKKIESDGYFS"
     gene            2157..2588
                     /locus_tag="PKJ17_00770"
     CDS             2157..2588
                     /locus_tag="PKJ17_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74525.1"
                     /translation="MKTLSGGKKALPGGNPFLEKVRTKYEIPDRIEIFAPRGGSEIIA
                     LVFDSEHLPVVDGKWSEITPDDYKYLQSLHNLKAVYCLHEKEPLDLGDVVVAIRPEGS
                     GALSIYFNDKQGTVGSARIRGGNAILFSGSKHIVRPMAAGL"
     gene            2591..3745
                     /locus_tag="PKJ17_00775"
     CDS             2591..3745
                     /locus_tag="PKJ17_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74526.1"
                     /translation="MRKLSRPAIMITLGVVVLAVILSVMVGMLLKNTSKKTRISQRGV
                     NQSGSPSKNGREKGGQEEESRIVDETKTTESTIGDAIRKEQQMLKESDKGGYIGGVGV
                     GKKKKESDLVLDDIVVEEPDGDDPLDPPVSGNGEGRKSIYNDAAGRGKRGKAAGERDD
                     GESKRKAWEYLYFQKSSGGFVNKPQPVAKDQDGEKPGSPGAAGGPKVSYYKPYRAVVD
                     RTFTSAQANATFVATGAEPPLKGWKVVGRAAANFTDNRFHVEVSGVIGPDNAHHQMKG
                     YVASIDETDGIVSAVKHEDVAGTVVSGVMAGVSSFLNALRKDTTTVEVGAGGVTVTGQ
                     TKSDDRLREGGLAAGDTIFQGLSKKVEGSTKKSPTLIMEKGVPVLIYFAP"
     gene            3763..4488
                     /locus_tag="PKJ17_00780"
     CDS             3763..4488
                     /locus_tag="PKJ17_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74527.1"
                     /translation="MRTKLLLQVLIIIAAVAGCSGLEHAGQGPGDPSSAATAADMWNI
                     LDGAAASRPYSRYLYRMKRADGSEGESGVVSMAQGGNENMNLVVTGGELRIVPAESFR
                     RSYDWETSASDRVPFGGGYAAGGRLAIIPGIYTPVDLYPSWFPTNAYVSVDEKCNLLV
                     RMKKTYENETQRLVAFMDARSKLEGLTCEVLGTFHTVPQLKKLVITLRKEGKYNHERH
                     CVLRLSWSSTYALVAISREGGRP"
     gene            4563..5111
                     /locus_tag="PKJ17_00785"
     CDS             4563..5111
                     /locus_tag="PKJ17_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74528.1"
                     /translation="MKGMSSELEDAFLDKPQTVKSESRIDPRTASGGEERSLTEGEKW
                     QARLAVFLDIPAVPPPDGHNLKLLEAPGTRFIVAVFDTGAERVVHRAKQIIWPRVRML
                     FAGGSTAFLHIVPAAGGTFSAGRTFYTIMSDKDGFSVFYIPQKSARKVALTFSGEEGA
                     FLTPDGFVCRIKRHTSDGGGIQ"
     gene            5092..6543
                     /locus_tag="PKJ17_00790"
     CDS             5092..6543
                     /locus_tag="PKJ17_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74529.1"
                     /translation="MEEESSEGKNIFRWGLAMAVFAAVCAPGWRANSAETVDGAFRRA
                     LLSEAVVKDARTKTVNLHFNGVPLNDALLSLGRQAGASITIDREIDTSKVKVSAYYEG
                     ESLEEAIETIVSGLDYAYRKVADGAYVVTPYEEAILNVHDVYVRHKESLSETSSSNNR
                     SLNGTSSTSTGVGYANSGTSANTSGTSSTGDKAEDREMSKIIESIKKMLSAKGVVTPM
                     TSGFVYVRDIPSRIKMVREMLDTDLRKRKTVTMKITLVRIDYKDEYETGLNWYAMLKS
                     RSPSVVLSTNFTQTMGDSNVATLLVIDEDFAGFIKMLGTYGDVHVVHSWESRAVSGAV
                     LPFEMSQTVWYSTGSVIQVVNNQTITTPQMASMEVGLKMTVNPLRLDKGYLVNTSVDL
                     SNLLGFQVVEKTELPQVERNYVSIPIKMSVGETVAISGFKIKSADKKRVGIPLLSNLP
                     VLSYLFGYNSSRDRTSELTVLINFVEEKGDREI"
     gene            6545..>7685
                     /locus_tag="PKJ17_00795"
     CDS             6545..>7685
                     /locus_tag="PKJ17_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74530.1"
                     /translation="MKLYVKFDDNVVFDVVTQKMVDPKTVRPGRGAKIIPFAEHISVR
                     KLKPREKLSHKIGDRWIASRRVSLAYLMAHYMVKNKKTVLAGFFDRGDGKYVFMEIKT
                     GNMTIEARYMLSVDSSPGNFMAMAGRQYDGIFGDASLSMEPDYVDISYEKLLKEVTIG
                     VTPLQAVVWLVVVAVIMVAVLNYAGVFAKRKTEPGAKAGATKEIPPLTHEEIRALSIL
                     ITGEALVKYKLYVETLPEDIALRNAVFRILPVAGSPRGKGREQELKGTLSFQFESFYP
                     FRESKKSGDLFVFGKEIVFSKGRADMRAALNAEARARQNTKAFETLVDLCDVAARGDR
                     EWKFTVAQKDYRDVVRIFNDIYLSPVVINTITVNGGNTTGELTCHR"
BASE COUNT         1917 a         1919 c         2310 g         1539 t
ORIGIN      
        1 gatcctgcaa aacgtggatt tccccatggt aatgaacact acccttctgt gcgaggacct
       61 gtcgaccatt acccgcgatc tggcgcggca aagactgatg gcaaatatct tcagcgggaa
      121 acggacagcc gcagcagtag agaacaagga gaagatcacg gccatcgacg atcttctgca
      181 ggaccgggcg agacacggct ggaagctcgt caagtgtttc aacagctatc ttgtatggga
      241 caaggacatt gaaaagcttc aggagaagat ccaggcagtt cgtgtggccc tggccaaggc
      301 ggtagacggg gcaggcgtgt tcgccgaatg gatgagaaag gagggggcct tcattgcatc
      361 ccttccaggg tgtggcgtga ggagttatga cccgcacatc gtctttggtc atgatgtgtt
      421 gaagcttgtc ccgttgcgcg ggatgtaccg tggcgatcgc tccgagccgg cgatcgtcct
      481 gagaaaccgc tacggcggcg ttacggcgct caatcccttc agttcaaggc agaacagatg
      541 ggcgggcctt gtcatcgggc ccacgggcag cggaaagtcg gtactcacca acgggatcat
      601 tgccggggcc atggcctatg accctgttgt tgtggtcatt gacatgtcga aggcctcttc
      661 gtatgaacct gttgtctcca attttggcgg cagctttatc cccatcacct tcggcagcaa
      721 tgacaacagg gtcaatatgt ttgacctgag ggtgggattc gagcagcccg caggatcgaa
      781 gatcatgtct ctcaacgcca ttttcacggc aatgctcggc gaggagggaa gaggtgttgc
      841 caaagagacc caatccatcc tggagcgggc tgttagaagg atgtatgaca atcttttcaa
      901 agaggaaccc cggcttgtgc ggaacgtaag aatagtgaac ggaacccagg aagagatctt
      961 ctcggacgca ccttcctttg acacatatat tgagtatcgt aaccattaca ttgaacggtt
     1021 cctgaagacg ggtgagagaa aggattttct gcgggcggag acggcgcaga gcctcgccac
     1081 cccgacactc aacgacctta tgcggatcct cgcgtcggac ccggcgatca acacaaatgc
     1141 cagggacaga cagatcgccg aagagatgag aaggatcctc aacctgtacg cgcagggaga
     1201 gcaggctgcc ttgttgaacg gggtaacgaa cttctccgtg gacagggata tattttgcat
     1261 tcacatgggc ctcgtcagcg agagaaagga gatgcttgcg ctcctgttgc tcctctaccg
     1321 ggacttcgcg tatcgcaagg cggtctatct cccaaacgag atgccgccct ttctcggtgc
     1381 cgcctctatc gactggatcc tcgctgctca gaggcggccg aagctctttg tctatgacga
     1441 gttccataat ctgaagaaca acgagcccat tctggacgtg ctcgacaagg acgcaaggca
     1501 gcaaaggaca ctggggcttg cgacctatct tgtgacacag gatatcctcg atgtggcgga
     1561 gaagggcaat catttcatct ccgccgcatc gaacaagtat ttcacacgcc acgtgtcccc
     1621 cgacaatccc acaatggaag gcatcgacaa tctggtgagg atcattggcc tcggcagcca
     1681 ggaaagggag cttctcctct cgctcaagtt ctatcccggc aaatacgccg agatcctggc
     1741 gctcaacgag gatatcggca agggggttct tgtcaacgta cagacgcccc gaacccggtg
     1801 gagctacacg acccacaagg acgaacgcta tctgagggaa accgtggcat cgacgctcaa
     1861 ggagaagggg gtgtcacaaa ggcgtgctca agcggttgcc gtcgaggtcc tctcggacat
     1921 gcttcctctg gggagcatcg gacagtccgt cgatatcggt gccgtaatga agaagatcga
     1981 gtcagacggc tatttttcat aagggaacaa gaggggaagg ggggacacca tcgttcttcg
     2041 catgctgatc gtaatgctcg caatcgtcgc cacgggtgct ttaccggtgc cggcaatgga
     2101 gagaccgcag acggatagtt ccgccatgga tgccctgggg ggagttcctc agaggaatga
     2161 agaccctttc gggcggaaag aaggcgcttc ccggagggaa tcctttcctg gagaaggtgc
     2221 gtacgaaata cgagataccg gacaggatag agatcttcgc tccccgcggc ggaagcgaga
     2281 tcatcgccct tgtcttcgat agtgagcatt tgcccgttgt ggacgggaaa tggagcgaga
     2341 tcacccccga cgattacaaa tatcttcaat cgctgcacaa tttgaaggcc gtctactgcc
     2401 tccatgaaaa ggagcccctc gatctcgggg acgtggtggt tgccatcaga ccggaaggct
     2461 ctggagcgct gtcgatatat ttcaatgata agcagggcac cgttggatcg gcaaggatac
     2521 ggggtggaaa cgcgatactc ttttcaggct cgaagcatat cgtccgacct atggcggcag
     2581 ggctctgacg atgaggaagc tctcaagacc cgcaattatg atcacgctgg gcgttgtcgt
     2641 gctggcggtg atattgtcgg tgatggtcgg catgctcttg aaaaatacgt ccaagaagac
     2701 gcgcatctct cagcgtgggg tcaatcagtc ggggagtccg tcgaagaatg gtcgagaaaa
     2761 gggcggtcag gaggaggaat cccggatcgt ggatgaaacc aaaacgaccg aaagcaccat
     2821 aggggatgcg atcaggaaag aacagcagat gctcaaggaa agcgacaagg gcggatacat
     2881 cggcggggtt ggtgtgggaa agaagaaaaa ggaaagcgat ctggtcctgg atgacattgt
     2941 tgtggaagag cccgatggcg acgatccttt ggatccgccc gtgtcgggga acggtgaggg
     3001 caggaaaagc atttacaacg atgccgccgg acgaggaaaa aggggaaagg ccgccggaga
     3061 aagggatgac ggtgaatcaa agagaaaggc atgggagtat ctctatttcc agaaaagctc
     3121 cggtggtttt gtcaacaaac cgcaacccgt cgctaaagat caggacggcg agaagccggg
     3181 ctccccgggc gctgcggggg ggccgaaggt gagctactac aagccctacc gggcagtagt
     3241 ggatagaacc tttacctcgg cgcaggcaaa tgccacattt gtggccaccg gggccgagcc
     3301 acccctgaaa ggctggaaag tggtcggtag agctgcggcc aatttcaccg ataacaggtt
     3361 ccacgtggag gtgtccgggg tgatcggccc cgataacgcc catcatcaga tgaaaggata
     3421 tgtggcatcg atagatgaga ccgatgggat cgtatcggca gtaaagcacg aggatgtcgc
     3481 cggcacggtc gtgagcggag tcatggctgg agtctcgtca tttctcaacg cgctcagaaa
     3541 ggacaccacg acagtagagg taggagccgg aggcgtcacg gtaacgggcc agacgaaaag
     3601 cgacgatcgc ctgagggaag gcgggctggc ggcgggagac acgatctttc aggggttgtc
     3661 gaagaaggtg gaaggctcga cgaaaaagag cccgaccctc attatggaaa agggagttcc
     3721 cgtattgatc tatttcgcac cgtaggtcgg ggaaaagaca agatgcgtac gaaacttctc
     3781 ctccaagttc ttattatcat cgccgcggtg gcgggctgta gtggtctcga gcatgcggga
     3841 caaggcccgg gcgatccatc gtcagcggca acggccgccg acatgtggaa catcctcgat
     3901 ggtgcggcgg cgtcaagacc ttattcccgc tacctttaca ggatgaaaag agcggacggg
     3961 agtgaagggg aatcgggtgt cgtgtcgatg gcgcagggag gcaacgaaaa catgaatctt
     4021 gtcgtcaccg gaggagaatt gcggatagtg ccggctgaat cctttcggag gtcctacgac
     4081 tgggagacat cggcgtcgga ccgcgtgccg ttcgggggag ggtatgcggc cggtggcagg
     4141 cttgcgataa tacccggcat ctacacacct gtcgaccttt acccgtcatg gtttcctacg
     4201 aacgcgtatg tgagcgtcga cgaaaaatgc aatcttcttg tccggatgaa aaagacgtat
     4261 gagaacgaaa cccagagact tgttgccttt atggatgcca ggagcaagct ggagggcctg
     4321 acatgtgagg tgctcgggac cttccacact gtgccgcaac tcaagaagct ggtgatcaca
     4381 ctcaggaagg aagggaagta caaccatgaa cgccattgcg tgctccgcct gtcatggagc
     4441 tcaacctatg cccttgtcgc catctcccgc gaaggaggga ggccatgagg cgcattcttg
     4501 tgaccctgtt tgcggcatgc atgtgtcttt gtgcatattc agggcaaggc gagaccatgt
     4561 cgatgaaagg aatgtcttcg gaactggaag acgcctttct cgacaaaccg cagaccgtca
     4621 aaagtgagag ccgaatagac ccgcggaccg cctcgggggg agaagaacgg tctttgacag
     4681 agggtgaaaa atggcaggcg agacttgcgg tctttctcga catacccgcc gtgccgccgc
     4741 ctgatggcca caaccttaaa ctgcttgagg cgccaggcac caggttcata gttgccgttt
     4801 tcgataccgg cgcagaacgt gtagtccaca gagcgaaaca gatcatatgg cccagggtaa
     4861 ggatgctttt tgccgggggc tcaacagctt tcctgcatat agtgcccgcg gcagggggaa
     4921 ctttcagtgc cggcaggaca ttctacacca tcatgagcga caaggatggt ttcagtgtgt
     4981 tctacatacc gcaaaaatcg gcgagaaagg ttgcgctcac attttcaggg gaagaggggg
     5041 catttctaac acccgacgga tttgtctgcc ggataaagag acatacatcc gatggaggag
     5101 gaatccagtg aggggaaaaa tatcttcagg tggggcctcg caatggcggt ctttgctgcg
     5161 gtctgcgccc ccggatggcg tgcgaacagc gccgaaacgg tggatggagc ttttaggaga
     5221 gccctcctgt ccgaggcggt tgtgaaggat gcgcgaacga agacggtgaa ccttcatttc
     5281 aatggtgtgc cgcttaacga tgcccttctt tctctggggc ggcaggctgg ggcaagcatc
     5341 accatcgacc gcgaaataga cacctcaaag gtgaaggtaa gcgcttatta cgaaggagaa
     5401 tccctcgagg aggccatcga gaccatagtc tccggtcttg actatgctta cagaaaggta
     5461 gccgacgggg catacgttgt caccccctac gaggaggcca tcctcaatgt tcatgacgta
     5521 tatgtgaggc ataaggaatc gcttagcgaa acttcgtcat cgaataaccg gtcgctgaac
     5581 ggaacatcat cgacgtcgac aggtgtggga tacgcaaatt cgggaacgtc ggcaaacaca
     5641 tcgggaacct catcgacggg ggacaaagca gaagaccgcg agatgtcgaa gatcatcgaa
     5701 agcatcaaga aaatgctttc ggccaagggt gttgtcacgc cgatgacgag cggttttgtc
     5761 tacgtgagag acataccgtc acgcatcaag atggtcaggg aaatgctcga cacggacctc
     5821 aggaaacgca agaccgttac catgaagatc acccttgttc gaatagacta caaggatgaa
     5881 tacgagacgg ggctcaactg gtacgcaatg ctcaagtcga gaagcccctc agttgtattg
     5941 agcacgaact tcacgcagac gatgggagac agcaacgtcg ctaccctgct ggtgatcgat
     6001 gaagatttcg cgggcttcat aaagatgctc gggacctatg gcgacgtcca tgtggtccat
     6061 tcgtgggaat caagggccgt ctcaggtgca gttcttccat ttgagatgtc acagacggtc
     6121 tggtactcaa cgggcagcgt gatccaggtt gtcaacaacc aaacgatcac gacaccgcag
     6181 atggcaagca tggaagtagg actcaaaatg accgtcaatc ccctgaggct ggacaaaggg
     6241 tatctcgtaa acacgagcgt cgatctttcc aatctccttg gttttcaggt cgtggagaag
     6301 accgaactcc cccaggtaga gcgcaattat gtttcgatac ccatcaagat gtctgtcgga
     6361 gagacggtcg ccatatcggg tttcaagatc aagagcgccg acaaaaagcg ggtgggcata
     6421 cccctcctga gcaatttacc cgtcctttcc tatctattcg ggtacaacag ttctcgtgac
     6481 agaacctccg agctgacggt gctcatcaat ttcgtcgagg agaagggcga cagggagata
     6541 tgaagtgaag ctctatgtga agttcgacga caatgtcgtt ttcgatgtcg ttacacagaa
     6601 aatggttgat ccgaagacgg tcaggccggg caggggggca aagatcatcc cttttgcgga
     6661 gcatatatcc gtacggaaac tcaaaccccg ggaaaaactc tcccacaaga taggcgaccg
     6721 atggatagcg tcgcgacgcg tgtctttagc gtaccttatg gcccattaca tggtgaagaa
     6781 caaaaagaca gtgctcgccg ggttctttga ccgcggggac ggcaagtacg tcttcatgga
     6841 gatcaaaacc gggaacatga ctattgaggc ccgatacatg ctgagcgtgg acagcagccc
     6901 cgggaacttc atggccatgg caggaagaca gtacgacggg atattcggcg atgcttccct
     6961 ttccatggaa ccggattatg ttgatatctc ttacgagaag ctccttaaag aagtgaccat
     7021 cggggttact ccccttcagg ccgttgtgtg gcttgtcgtt gttgccgtca tcatggtcgc
     7081 agttctaaac tatgcgggtg tctttgcgaa gagaaaaacg gagcccggcg cgaaggcagg
     7141 agcgacgaag gaaataccac ctcttaccca tgaggagata cgggcattgt cgatactcat
     7201 caccggcgaa gccctggtaa agtacaaact ctacgtcgaa acacttcccg aagacatcgc
     7261 cttgaggaac gctgttttcc gcatcctgcc cgtcgcaggc tcaccccggg gaaagggacg
     7321 ggaacaggag ctcaaaggca cattgtcttt tcagttcgag tccttctatc cctttcgaga
     7381 aagcaaaaag agcggcgatc tctttgtctt cggaaaggag atcgtgttct cgaaaggcag
     7441 ggctgacatg cgggcggcgt tgaacgctga ggccagagcc cggcagaata ccaaggcctt
     7501 cgaaaccctc gtggaccttt gcgatgttgc ggccaggggg gacagggagt ggaagttcac
     7561 ggtggcccag aaggactaca gggatgttgt gaggatattc aacgacatct acctgtcgcc
     7621 tgttgtgatc aacaccatca ccgtcaatgg gggaaacacg acgggagagt tgacatgcca
     7681 caggt
//
LOCUS       DAOPOA010000020         7452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_11025_length_7452_cov_3.551575, whole genome shotgun sequence.
ACCESSION   DAOPOA010000020 DAOPOA010000000
VERSION     DAOPOA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11025_length_7452_cov_3.551575"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKJ17_00800"
     CDS             complement(<1..361)
                     /locus_tag="PKJ17_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOD74531.1"
                     /translation="MEILVLYHSRSGNTKKLAQAIAAGVESVTGASCVMKDTKEVGQE
                     DFLRADGIIAGSPVYFGSMAAELKEVFDTFVPVRKKMGDKVGAAFATSGDLSGGKETT
                     MMSIIQALLIYGMIVVGD"
     gene            complement(574..1014)
                     /locus_tag="PKJ17_00805"
     CDS             complement(574..1014)
                     /locus_tag="PKJ17_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74532.1"
                     /translation="MRKRPMPAAASVLIVVLLLLAAASVGAFPGEPDGYNGIPWGTPL
                     ESLTSMEYAGPGKDSPGTALYRRVTDDLTFGKARLTRIEYGFVAGRLTAVTLKVDSLL
                     QYLLMKEEAVRRFGPGTEADPRAERLIWEGDSTTIRLVSAFDLS"
     gene            complement(1011..1535)
                     /gene="hpt"
                     /locus_tag="PKJ17_00810"
     CDS             complement(1011..1535)
                     /gene="hpt"
                     /locus_tag="PKJ17_00810"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOD74533.1"
                     /translation="MLKPLFSKRDIARTVRELARRIEKDYSGEEIVFVCLLKGSFMFT
                     SDLVRYIKNPSRVDFMRVSSYGNAMKTNGRVNILKDLEEDIEGKNVIIVEDIIDSGLT
                     LTHVRRLLGDRKPRSLKICALIDKRCRREVEIEGDYVGFTIDDGFVVGYGIDYAERYR
                     NCPDICVVVEGDDE"
     gene            complement(1555..2211)
                     /locus_tag="PKJ17_00815"
     CDS             complement(1555..2211)
                     /locus_tag="PKJ17_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD74534.1"
                     /translation="MKIKWYGHSAFLITTEKGVRIITDPYQSGAFGGDLAYGKITDEA
                     DIVLSSHDHDDHNYTKDIKGRFTYINRSGVYEEKGVKIRAIPTYHDGSKGSQRGKNLV
                     FVVEADDLKVAHAGDLGHTLDAAAVKDVGAVDILLLPVGGFFTIDASEAARIVEDLKP
                     RVTIPMHFKTDKCAFPITPVEDFTRGKEGVRDAGVTEVEFSKSGLPDKAEIVVLRHAL
                     "
     gene            2542..3171
                     /locus_tag="PKJ17_00820"
     CDS             2542..3171
                     /locus_tag="PKJ17_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD74535.1"
                     /translation="MTDAKDCCVYLLNLGELVLIDAGAGISAGAIVRNIESLGFDAAL
                     VSTIVLTHCHIDHVGGAAAIKERTGARIVMHELDAVPVENGDQRMTAARWYGVRFQPL
                     PVDLTFSGHSETFSFGGGDLILLHTPGHTPGSLSVYCDTGGKRVLFGQDIHGPFLADF
                     GANMSHWQRSMEELLALNADILCEGHFGIYQPNKKVRAYIERYIDEYGE"
     gene            3161..3811
                     /locus_tag="PKJ17_00825"
     CDS             3161..3811
                     /locus_tag="PKJ17_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOD74536.1"
                     /translation="MESKYRLGPHVSRFTFRALPPLPRRSPRSSTLLLRRKGEEATSR
                     LTRNISSFQGSAPVIHDTAFIDDSARVIGDVLVGEGVSIWPMAVVRADEAATVIGKGS
                     AILDLSLLESPAGLPVAIGEGSIISHGAIVHGASIASGVLVGAGAMVLDNAVVGEGSI
                     VAAGAVVTPRTVIPPNSLVLGSPGRVARATTLEERQGVTKQARGLFRKSRAYLAEP"
     gene            3986..4846
                     /locus_tag="PKJ17_00830"
     CDS             3986..4846
                     /locus_tag="PKJ17_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOD74537.1"
                     /translation="MELQGSSDIVRFQRASDFDGLEFLHATFVQHSFPRHTHDTFAIG
                     VIESGVQATYYKGSTHIATCRDICLINPGEVHTGFSPHPSGWTYRVFYPEPSLLETVA
                     SQLLPREHGLPHFSSPVIKDRLLSTAVLSFLRVLDLSDVTIERESLLYAVIAQMISRH
                     ADRKGPMSPVPRHAERPVVKTLLEYLHHHFTDNVTLRELSDVAALSEFHLLRLFRSAV
                     GLPPHAYIIQKRVDHARRLLLTGLPIAHVGIEAGFADQSHFTRAFKRIVGVTPGCYRS
                     SNSVQDFSDL"
     gene            4922..5416
                     /locus_tag="PKJ17_00835"
     CDS             4922..5416
                     /locus_tag="PKJ17_00835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD74538.1"
                     /translation="MIRICADRDFDSILEIINDGARAYKGIIPHDCWHEPYMARPELS
                     HEIADGVVFYGCEEEGVLVGVMGIQDRGDVSLIRHAYVRTARRNCGIGTRLLGFLQST
                     TTKPLLVGTWAAAAWAIAFYEKRGYKVLPPHDKDRLLRKYWNIPRRQVDTSVVLAGPG
                     WPHP"
     gene            5869..6036
                     /locus_tag="PKJ17_00840"
     CDS             5869..6036
                     /locus_tag="PKJ17_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74539.1"
                     /translation="MGPLATRFLFLFGLFRNYSVAWRVCVAVSLMAFILSSSTGRVRR
                     SDMGTVREGGH"
     gene            complement(6109..6792)
                     /locus_tag="PKJ17_00845"
     CDS             complement(6109..6792)
                     /locus_tag="PKJ17_00845"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOD74540.1"
                     /translation="MNIRPGLNINIIINIDHMRETVDVGNSIIHEMAGDRMIVAQTDP
                     PVSRTHLGKELFVTFLDRSGGEPHRFGFPAIVLEFLKEFELSASLKKVQALVLRKAGD
                     FEEYNLRMFYRLEPPSDCGIRIAVDGKGVNLIDISIGGAKFSHEKVFPFEIGEPVRLS
                     LYVDEVAYPVEAKVLRVWEPENERIRKTVALASVQFTDMEAYLKNVLARKIRDVEREM
                     RYKELHPAR"
     gene            complement(6826..7194)
                     /locus_tag="PKJ17_00850"
     CDS             complement(6826..7194)
                     /locus_tag="PKJ17_00850"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOD74541.1"
                     /translation="MIVRNFNDPEVLETRYRAHWGATAWMVMTSRFLEGIEFLAYAIL
                     PPGNTIEEHVDEVEEIYMIFRGGGKMKVGDEIREVKEGDSIWIPAGEPHALENTKEEE
                     TFVLVIAAYPFKRFLQMAEK"
     gene            complement(7273..>7452)
                     /locus_tag="PKJ17_00855"
     CDS             complement(7273..>7452)
                     /locus_tag="PKJ17_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74542.1"
                     /translation="RSLPGSLYEAIELTEGSKLVRDALGDHVFEKLIENKKIEWDRFR
                     AHVSNYEIETYLPVL"
BASE COUNT         1354 a         2429 c         1993 g         1676 t
ORIGIN      
        1 ggtcgccgac gacgatcatg ccgtagatga gcagggcctg gatgatcgac atcattgtgg
       61 tttcctttcc tcccgaaagg tccccggagg tggcaaaggc cgcccccacc ttgtcgccca
      121 tcttcttccg gacgggaacg aacgtgtcga agacctcttt cagctcggct gccatggagc
      181 cgaagtagac cggggaaccg gctatgatcc cgtcggcgcg caggaagtcc tcctgtccga
      241 cctccttcgt gtctttcata acacaggagg ctccggtgac ggactccacc cctgccgcta
      301 tggcctgagc cagcttcttt gtgtttcccg atcgcgaatg gtacagtaca agaatctcca
      361 tgaaacctcc tttagacgat agtgaccgat gaccggactc caatgatcag atagtgccgg
      421 gggtacccgt acccccgttc ggggatagac gaacagggag gggaagccgg cggagggaac
      481 ggcatatcgc ttcccgcatc cctttgacca ttggaatctg ctcgctggtc attttctttt
      541 tatctcgagg aatccttccc cgtgggcacc gtctcaggac aggtcaaagg cgctcacgag
      601 ccttatggtc gtgctgtcgc cctcccagat gagcctttcg gccctgggat cagcctccgt
      661 gcccggcccg aaccttctca cggcttcctc cttcatgaga agatactgca gaagcgagtc
      721 aaccttgagg gtaacagctg tcaaccttcc ggcgacgaat ccatactcga tccttgtcag
      781 gcgggccttg ccgaaggtca ggtcgtccgt aacgcgccga tacagggctg tgccggggga
      841 gtccttcccc gggccggcgt attccatcga cgtcaggctc tcgaggggtg tcccccaggg
      901 gatgccgttg tatccgtccg gctctccagg gaacgcgccc acactcgcgg cggcgaggag
      961 caacaacacc actatcagga cgcttgccgc cgccggcata ggtcgcttcc tcattcgtca
     1021 tccccctcca cgacgacaca gatgtcgggg caattcctgt atctctccgc gtagtcgatc
     1081 ccgtagccca caacaaagcc gtcgtctatg gtaaaaccga cataatcgcc ctcgatctcc
     1141 acctctcgcc tgcatctctt gtctatgagc gcgcagatct tgagcgacct cggcttccgg
     1201 tcgcccagga gacggcggac gtgcgtgagc gtaaggcccg agtctatgat gtcttcgacg
     1261 atgatgacgt tcttgccctc gatgtcctcc tcgaggtctt ttaggatgtt caccctgccg
     1321 ttcgtcttca tggcgttgcc gtatgaggac acgcgcatga agtcgacgcg cgaggggttc
     1381 tttatgtagc ggaccaggtc cgacgtgaac atgaaggagc ctttcagcag acacacgaag
     1441 actatctcct cgccgctgta atccttttct atccgtcttg caagttccct gaccgttcgc
     1501 gctatgtctc gtttcgaaaa aagtggtttc agcatccagg tctcccgtgt gccgctagag
     1561 ggcatggcgc aacacgacta tctccgcctt atcggggagg ccggacttcg agaattccac
     1621 ctccgtcacg cccgcatctc taaccccttc cttgccccgc gtgaaatctt ccaccggcgt
     1681 tatggggaag gcgcacttgt ccgtcttgaa gtgcatgggt attgtgacac ggggttttag
     1741 gtcttccact atccgcgcgg cctccgacgc gtctattgtg aagaacccgc ccaccggcag
     1801 cagcagtatg tccacagccc ccacgtcttt cacggccgcc gcatcgagtg tatggccgag
     1861 atctcccgca tgggcgacct tgaggtcgtc cgcttccacg acgaagacga ggttcttccc
     1921 ccgctgactt cccttcgatc cgtcatggta cgtcgggatg gcccgtatct tgaccccttt
     1981 ttcctcataa acccctgatc tattgatgta agtgaaccgc ccctttatat ctttggtgta
     2041 attgtggtcg tcatgatcat ggctcgaaag cacgatgtcc gcttcatcag tgatcttccc
     2101 gtaggccaga tcccccccga atgcgccgga ttggtacggg tcggttatga tcctcacgcc
     2161 cttctccgtc gttatgagaa atgccgaatg accgtaccat tttatcttca tccctatcct
     2221 cctttttttc ctttatgtta cgactacttt cgcccactgt caagacgcgg ggccgtcgct
     2281 cggcgcccca ggtggccgaa cccgtggaaa agtcttgcaa tagtcgggaa gagaggtaga
     2341 ataaagacag ttttaagttt taagtgttaa gttttagatt agcgactgtg ccccggaccc
     2401 gtaaggtttt ccgcgtgtgc ctgcaccgcc tcttgtcttc ttttccttaa agcttaaaac
     2461 ctgtttctga aaatgtcgtc cggagttgtc acgagaccga aagagataca taacggcgtt
     2521 tatctcgtcg gcggttcaga tatgaccgat gcaaaggact gctgcgtcta cctcctcaac
     2581 ctcggagagc tggtgctcat cgatgccggg gcgggaatca gcgcgggcgc catcgtccgc
     2641 aacatcgaat ctctcggctt cgacgccgcc ctggtctcca cgatcgtcct cacccattgc
     2701 cacatcgacc atgtaggcgg cgcggcagcc atcaaggaac ggacaggagc tcgcatcgtc
     2761 atgcacgaac tcgatgccgt gcctgtcgaa aacggcgacc agaggatgac ggccgcccgc
     2821 tggtatgggg tcaggttcca gcccctgccg gtggacctca cgttctccgg ccatagcgaa
     2881 acgttctctt tcgggggggg cgatctcatt ctgcttcata cccccggtca cacaccgggc
     2941 tcgctctccg tctactgtga caccggcggc aaacgcgtcc tcttcgggca ggacatacac
     3001 ggtccttttc ttgccgattt cggggccaac atgtcccact ggcagaggtc catggaagaa
     3061 ctcctggcac tcaacgccga catcctctgc gagggccact tcggcatcta tcagccgaac
     3121 aaaaaggtcc gcgcttacat agaacgctat atcgatgagt atggagagta aataccgtct
     3181 tgggcctcat gtctcccgtt tcacgttcag ggctctgccc ccgttaccac ggcggtcgcc
     3241 ccgctcctcg accctcctcc tccgcagaaa aggtgaggaa gcgacctcca ggcttacgcg
     3301 caatatctca tcatttcaag gcagtgctcc tgttattcat gataccgctt tcatagatga
     3361 ttcggcccgg gtgatcggtg atgtccttgt cggggagggg gtcagcatct ggccgatggc
     3421 ggtcgtgagg gccgatgagg ccgcgacggt catcgggaag ggatccgcca tcctcgatct
     3481 gtccctcctg gaatccccgg cggggcttcc ggtcgccatc ggagagggat ccatcatcag
     3541 ccacggggcc atcgtgcacg gggccagcat cgcgtccggc gtcctcgtgg gcgccggcgc
     3601 catggttctt gacaacgccg tcgttggtga agggtccatc gttgccgcag gtgccgtcgt
     3661 gacgccccgg actgtcatac cccccaattc gcttgtcctc ggttcgccgg gcagggtcgc
     3721 gcgcgcgacg acgcttgaag aaaggcaggg cgtcacgaag caggcccggg ggcttttccg
     3781 caagtcgagg gcgtatctgg cggagccgtg aggcaggctc aagggaaaga ccgcggggtt
     3841 gagcgactgt caagacaatt gccggtcaga gcgtgtcgct gccatcctcg cgcacggaga
     3901 atgcgctctt tcagcggcaa gagtcttcta ggcgggggcg tctctttccc ggcacactgg
     3961 cagggagcgc cgcagggtag gtggaatgga attgcagggt agcagcgata ttgtcaggtt
     4021 ccagcgcgct tccgatttcg atggcctgga gttcctccac gccaccttcg tccagcattc
     4081 ctttccccgg catactcacg acaccttcgc cataggcgtc atagaaagcg gtgtccaggc
     4141 gacctattac aagggttcca cccatatcgc cacctgccgc gacatctgtc tcatcaaccc
     4201 gggcgaggtc cacacggggt tctctcccca cccgtcgggc tggacgtatc gcgtcttcta
     4261 ccccgaacct tcactcctcg agacagtcgc ctcccagctg ctcccccgcg aacacggact
     4321 tccgcatttc tcctcgcccg tcataaagga tcgcctcctc tccacggccg ttctctcctt
     4381 tctcagggtc ctcgacctct cggacgtcac gatcgagcgc gagtctctcc tttacgcggt
     4441 gatagcgcag atgatatcac gccacgccga caggaagggg ccgatgtctc cggttcccag
     4501 acacgccgaa aggcccgtgg tcaagacgct cctcgaatac ctccaccatc atttcaccga
     4561 caatgtcacc ctccgcgagc tttctgatgt cgccgctctg agcgagttcc accttctccg
     4621 cctattcaga tctgccgtgg ggctccctcc ccacgcctat atcatccaga aacgggtgga
     4681 ccacgcccgg cgcctcctcc tgacgggcct gcctatcgcc cacgttggca tcgaggcggg
     4741 ctttgccgac cagagccatt tcacccgcgc cttcaaaagg atcgtcgggg tcaccccggg
     4801 ctgctaccgc tccagcaatt ccgttcaaga cttctccgac ctttgagcct tacactctaa
     4861 ccaggctgcg ggagtccccg ccacccggcg cctcccgcag ccgcgccaag gaggaaccag
     4921 aatgataagg atctgcgccg atcgcgactt tgactcaatc ctggagatca tcaatgacgg
     4981 ggcccgggcc tacaagggca tcataccgca cgattgctgg cacgaacctt acatggcccg
     5041 cccggagctc agccacgaga tcgcggacgg cgtggtcttc tacggctgcg aggaagaggg
     5101 cgttctcgtc ggcgtcatgg gcatacagga ccgcggcgac gtgtctctta tccgccacgc
     5161 ctacgtccgg acggcgcgga gaaactgcgg catcggcacg aggctcctcg gctttctgca
     5221 atcgaccacc accaagccgc ttctcgtcgg cacatgggcg gctgctgcct gggcgatagc
     5281 cttctacgag aagagaggct acaaggtgct ccccccgcac gacaaggatc gcctcctcag
     5341 gaagtactgg aacatacccc gccgccaggt ggacacttcc gtcgtgctcg ccggccccgg
     5401 ctggccacac ccgtgatctc tccccggcgg cccccgttat tctccggcca ttttctcgtt
     5461 ccggccgggt tcatcatcat gctcgtcatg tagggcgcgc tccattcctt cggcgtctct
     5521 ctcaaacccc tccccgcgga acccggctga acgagagcgt ccccccttgg ttgcatattc
     5581 ttctctgctt cttccccggc ggcacactgg tggcaccgcc gaaatggctg accgacaagc
     5641 tcggcacaag gccggtcctt atcgccgcca ccctcatgat ggccgcccgg ttcggtctta
     5701 tcattgcatc ccgggatttc cgggttctcc ggtctcaacc agactcttcc gcatcaccta
     5761 tggggagatc ccttgcgcgg tgccgcttct tcccgctgag cggttcggcc tggatcatca
     5821 cggcgccctt tcggcgttac taccctcgcc agcaccctcg ggtacggcat ggggcctctg
     5881 gccacccgct tccttttcct tttcggtctt ttccggaatt acagcgtggc gtggagggtc
     5941 tgcgtggccg tctccctcat ggcgttcatc ctttcctcat cgacaggaag ggttcgccgc
     6001 agcgatatgg ggacggtccg cgaaggaggg cattgaccct gatgtttcac gtggaactac
     6061 cattttgttc aatgatctcg ctgtcttaca tcattaccgc gactgcggtc atcttgcggg
     6121 gtggagctcc ttgtagcgca tctccctctc tacatcgcga atcttgcgcg ccaggacatt
     6181 cttcaggtag gcctccatgt ccgtgaactg caccgacgcc agggcgacgg tcttccgtat
     6241 ccgctcgttc tcgggctccc acaccctgag gaccttcgcc tccaccgggt aagccacctc
     6301 gtccacgtac aggctcagcc ttaccggttc accgatctca aagggaaaga ccttctcgtg
     6361 gctgaatttg gctcctccga tggatatgtc gatgaggttc acacccttac cgtccacggc
     6421 tatcctgatg ccgcagtccg acggcggttc aagcctgtag aacatcctca ggttgtattc
     6481 ctcgaagtcc cccgcctttc tcaggaccag cgcctggacc ttcttgagag acgctgaaag
     6541 ctcgaattcc ttcaggaact caaggacgat cgccggaaaa ccgaagcggt ggggttctcc
     6601 gcccgacctg tcgagaaagg tcacaaagag ctccttgccc agatgggtcc tggatacggg
     6661 agggtccgtc tgggcgacga tcatcctgtc gcccgccatc tcgtggatta tggaattccc
     6721 gacgtcaacg gtctccctca tatggtctat attgatgata atattgatgt tcaggcctgg
     6781 tctgatgttc atgttcgcgt gtggacagga aaccgtcctt cctgtctatt tctcggccat
     6841 ctgcaggaat ctcttaaaag gatatgccgc tatcacgagg acgaaggtct cctcctcctt
     6901 cgtgttctca agggcgtgcg gctcaccggc cggaatccag atcgaatccc cctctttcac
     6961 ctcccggatt tcgtccccga ccttcatctt cccgccgccc cgaaagatca tgtatatctc
     7021 ttcgacctcg tcgacatgct cctcgatggt gttgcccggg ggcagtatgg catacgcgag
     7081 gaactctatg ccttccagga accggctcgt cattaccatc cacgcggtcg ccccccagtg
     7141 cgccctgtat ctcgtctcca acacctcagg atcgttgaaa ttacggacta tcatctgact
     7201 ccttccccgc ctgcagtcgt gtcgttttct ccgctcaaga ccatcgaacg ggtcaggcgg
     7261 ctgcaatcga ttctaaagga cgggcaggta cgtctcgatc tcgtagttcg acacgtgcgc
     7321 cctgaacctg tcccattcta tcttcttgtt ctcgatgagc ttctcgaaca catggtcgcc
     7381 aagggcgtcc cggaccagct tcgatccttc cgttaattct atggcctcgt acaggcttcc
     7441 cggaagggac cg
//
LOCUS       DAOPOA010000021         7271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_11375_length_7271_cov_3.544900, whole genome shotgun sequence.
ACCESSION   DAOPOA010000021 DAOPOA010000000
VERSION     DAOPOA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11375_length_7271_cov_3.544900"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="PKJ17_00860"
     CDS             <1..1513
                     /locus_tag="PKJ17_00860"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HOD74543.1"
                     /translation="MLIGDLPTQCARRYPDKTALVSDDNRLTYREFNKRVNCLADSLM
                     KLGLAAGDKVAVLSRNNVEMFEICFAAAKTGMVWVPVNFRLVPSELKFVLNDAEIGIL
                     FVGDTFKDQVEAIRNEITVPHIVDIGAPYEEMIRSGSPAEPAGSVKPDDLFAIFYTSG
                     TTGGPKGVMLSHENFLSAVVNHTIAYKLGPSDVSLHVQPCYHTMEASMVLCHFYVGGT
                     NVMVTNFNGHEFWKLVEKEKITNITLVYTGLVDILDAYKEGGYTLGTLKSYSVGGQTT
                     PVPILKRAVEVLGPDIIFVVYGLTEASPLVAYLPKEDYSLEDEGLKRLGSVGKELFSC
                     HVRIVDKDDNDVKPGEVGEIIARGPNVMKGYWKRPEETEETLRGGWLHTGDMGMFDED
                     GYIYVIDRKKDLIISGGENISPHEIEDALHLHPAVHECAAIGIPDERWGEQVKAVVVL
                     AKDTHVTEKELIEFCAKHLARYKLPKTVDFVDALPKDPVGKIQKKVLREQYAK"
     gene            1524..2030
                     /locus_tag="PKJ17_00865"
     CDS             1524..2030
                     /locus_tag="PKJ17_00865"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HOD74544.1"
                     /translation="MAIPSLDLMDQEQKNALLELVLWHYKVIDGFWFLHVSDAFGQSS
                     AESVVEKVWEDAARLASREIVKRFDIKEKGLAGFARAIAFFPMAALVGYDISPEDGAV
                     TISVPHCSAQEARLKHGLGEFKCKDMHRRLFEAFAKEIDPAIRTECLFAPPDPHPADM
                     FCKWRFTI"
     gene            2246..3421
                     /locus_tag="PKJ17_00870"
     CDS             2246..3421
                     /locus_tag="PKJ17_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD74545.1"
                     /translation="MQNRLWSKQFTAVISATLLLAWAFYALMPTLPIYLMKHLKMGQG
                     NIGMIMAAFSITAIIARPLAGFILDNHPRFRVLIISLIVSTAAYGFYPLVTGVAAMLI
                     LRLVHGAIWGTCSSANATVVADIVPPVRLGEGIGIFALCIPVGMTIGPMFGNEVLSGY
                     GPNAMFLSLLAVSLLAVIIALFARTPPRAITRTRFSIRNLFHRKALPLSLTMFFIMIA
                     YGAIIVFVGIYAGQRGFRNVTAFFLCFSASIFFSRLFLGRLFDRGYFHHLVVAGLTLT
                     AAGLPWLGLAATPLQFLGAGMVSGFGFGILMPTCQAAVNDLADVDERGAANSTYLVSY
                     DLGAGVGVLLIGFLAERIPLGRIYACCFFPILLSAGIFTFAALPHYLRNRSGNRGAG"
     gene            complement(3440..4609)
                     /locus_tag="PKJ17_00875"
     CDS             complement(3440..4609)
                     /locus_tag="PKJ17_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOD74546.1"
                     /translation="MPAGPVYLDNAATTFPKPVSVLEKAMETYRRIGVSPGRGGYDLA
                     AEAEEFVRETRRKAARFFGSSAPDRVIFTGNATDALNIAIQGMLHPGDHVVSTRLEHN
                     SVLRPLYHLRQKRIIEYDLVPFDGKGFVDPADIIAAIRPDTKLVVVTHASNVLGTVQP
                     IREIGRRCAEHGVPLLVDAAQSAGAVPIDVDGWQVAALAFTGHKSMLAPTGIGGIVLA
                     GQTEDIEPSRFGGTGIDSASPVHTRDFPYRLEAGTLNLLGIIGLSGSLDYLGEAGIEA
                     VHAREMELVTKLRDGLSLITGIDLYCAEDLSDHVALLTANVRGMDPHDVGAILDADFN
                     IAVRTGVHCAPLVHESLDTSPGGSVRFSVGPFNTGSDIDLTLEAMKEIARHGGKG"
     gene            complement(4615..4977)
                     /locus_tag="PKJ17_00880"
     CDS             complement(4615..4977)
                     /locus_tag="PKJ17_00880"
                     /inference="COORDINATES: protein motif:HMM:NF016261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOD74547.1"
                     /translation="MDGLSEPEERTWDLQQRVRVIAGKEVVFDDEGFLWRPEDWTEEV
                     GRMLASETGMEGLDDDQWRVIRFLREYYAYHGRAPMNRDLKAGLGMSLMDLEALFPGG
                     IRKGARRIAGLPNPRSCT"
     gene            complement(5246..>7271)
                     /locus_tag="PKJ17_00885"
     CDS             complement(5246..>7271)
                     /locus_tag="PKJ17_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOD74548.1"
                     /translation="RILSVDTSEAATMPGVVGIMTAPDIKGTNRIKIISADQPILAED
                     KIYCLGDAIAVVAARTKKEALDAAAAVRVEYDPLPVVTSPVEAMKEDAVRVHAESPNL
                     CFTQPQIKGDAEKALREAAALVEADFSTQINHQAPLEPEATVAYMEGDGDDAQLVVIG
                     RSINIHLHMADLQESLGYENVRYEEAFSGGNFGQKIAMTSEAISGAAALRFRRPVRYI
                     PSLAESMLMSSKRHAFAMKVRLGCGKDGKLTAFTIDLTVDNGAYQIIGISVVKRALWM
                     LSGSYSIPNVNAMARLVYTNNPAGGAARGAGPPQVTFALESAMDMLAAKMGMDALEFR
                     RINSLLPGQSCSTGMVYDEWPFPELCDAIRPAYERAKREAASLRPGPVRRGVGIASHA
                     FGIGTPADIARVVVELDPDDGLTVFAAVADPGEGNDSMLTQIASHLTGIPMDRIRLVT
                     RDTDRTTGMGPAAASRMTYMAGGSLVLAIEQLKKAMEEAGVDTYEGMKKAGKPTHYLG
                     SKMAAGKDANMDPETGQGLSFESRVHAIQMAEVEVDTDTGEVRVLRMTTAVDPGTVIN
                     PQNLEGQLHGGMDQGVGFALREQYIHGETKDWFTFKFPTMKTAFDMDVIIRETPRAKG
                     PLGAVGIGEMTMVCTAPAVINAICDACGVRIYDLPATPEKIRTALDEKTA"
BASE COUNT         1366 a         2363 c         2073 g         1469 t
ORIGIN      
        1 catgcttata ggcgatctac ccactcaatg cgcgcgccgg tatcccgaca agaccgctct
       61 ggtctccgac gacaacaggc ttacgtaccg ggaattcaac aagcgggtga actgcctcgc
      121 cgattcactt atgaaactgg ggcttgccgc gggcgacaag gtcgccgtgc tcagccgcaa
      181 caacgtcgag atgttcgaga tatgcttcgc agcggcaaag acgggcatgg tctgggtccc
      241 cgtcaacttc cggctcgtcc cctcggaatt gaagttcgtc ctcaacgatg cggagatagg
      301 catcctcttc gtgggcgaca ccttcaagga ccaggtcgaa gccatcagaa acgagatcac
      361 cgttccccac atcgttgaca tcggagcacc ctacgaggag atgatacggt cgggctcgcc
      421 cgcggaaccg gcgggcagcg tcaaacccga cgatcttttc gccatcttct atacgagcgg
      481 caccacgggc gggcccaagg gcgtcatgct cagccacgag aacttcctgt cggccgtggt
      541 gaaccacacc atcgcctaca agctcgggcc atccgatgtg agccttcacg tccaaccgtg
      601 ctatcacacc atggaggcat cgatggtgct gtgccacttc tacgtgggcg gaacgaacgt
      661 gatggtgacc aatttcaacg gtcatgaatt ctggaagctc gtggaaaagg agaagatcac
      721 caacatcacc ctcgtctaca cgggcctcgt cgacattctc gacgcctaca aggaaggcgg
      781 ctacacgctc ggcacactga agagctattc cgtcggcgga caaacaaccc ccgtacccat
      841 cctcaagcgg gcagtggagg tgctcggacc cgacatcata ttcgtggtgt acggcctcac
      901 cgaggcatct ccccttgtcg cctacctccc caaggaggat tattccctgg aggacgaggg
      961 cttaaagcgc ctcggctcgg tgggcaagga actcttcagc tgccacgtga ggatcgtcga
     1021 caaggacgac aacgacgtga aacccggcga ggtgggtgag atcatcgccc gcggtcccaa
     1081 cgtgatgaag ggctactgga aaaggcccga ggaaactgag gagaccttaa ggggcggctg
     1141 gctccatacg ggcgacatgg gcatgttcga cgaagacgga tacatatatg tcatagaccg
     1201 caagaaggac ctcatcatca gcggcggcga gaacatctcc ccccacgaga tcgaggacgc
     1261 tctccatctc catcccgcgg tgcacgagtg cgcggccatc gggatacccg acgaacgctg
     1321 gggcgagcag gtcaaggccg tcgtcgtcct cgcgaaggac acccatgtaa cagaaaagga
     1381 gctcatcgaa ttctgcgcga agcacctggc ccgctacaaa ctgccgaaga ccgtggattt
     1441 cgtcgacgcc cttcccaagg acccagtcgg caagatacag aagaaggtcc ttcgggaaca
     1501 atacgcgaaa taaaggaggc gttatggcca ttcccagtct cgatctcatg gatcaggagc
     1561 aaaagaacgc ccttctcgaa cttgtactgt ggcactacaa ggtgatcgac gggttctggt
     1621 tccttcacgt gtccgacgcg ttcggccagt cttccgccga aagcgtcgtc gaaaaggtgt
     1681 gggaggacgc agcgcgcctc gcctcccggg aaatagtgaa acgttttgac ataaaggaga
     1741 aggggctcgc cggtttcgcc cgcgctatcg cctttttccc catggcagcg ctcgtgggat
     1801 acgatatatc cccggaagac ggcgccgtta cgatcagcgt cccccactgc agcgcccagg
     1861 aggcccgcct gaaacacggc ctcggggaat tcaaatgcaa agacatgcac cgcaggctct
     1921 tcgaggcctt cgcgaaggag atcgatcccg cgataaggac agagtgtctc ttcgctcccc
     1981 ccgacccaca ccccgcggac atgttctgca agtggcgctt caccatctag accccctgat
     2041 tgaagcccgg cccccttcct gcccctccgg aacgggaccg ggccctcatc atcagcaccc
     2101 cgatctcttt cccatcacct ctcacccatc acccatgacc tgtttcattc tttttttgta
     2161 ttcccggaag ttttgtgata ggttcctgca tcactgaaag cgcaaggaag gctcatccct
     2221 ccagcagctc actaaaggat acccgttgca gaacagactc tggtcgaaac agttcacggc
     2281 cgtgatatcg gcgacattgc tcctggcgtg ggcgttctac gcgctcatgc ccactctgcc
     2341 catctacctg atgaaacacc tgaagatggg ccaggggaac atcgggatga taatggccgc
     2401 cttttccatc accgccatca ttgcccgccc ccttgcggga ttcattctcg acaaccatcc
     2461 ccggttccgt gtcctcatca tctccctcat cgtttcgacg gccgcgtacg gcttctatcc
     2521 attggtgacg ggggttgcgg ccatgctcat tctccgtctt gtccacggcg ccatatgggg
     2581 gacctgttcc tctgcgaacg ccaccgtcgt cgccgatatc gtgcctccag tacgtctcgg
     2641 tgaaggcata ggcatctttg ccctgtgcat tcccgtgggc atgaccatcg gccctatgtt
     2701 cggcaacgag gtgctcagtg gctatggccc aaatgccatg ttcctttcac ttctggccgt
     2761 ctccctcctg gccgtcatca tcgccctgtt cgcgcggacg ccccccaggg cgatcaccag
     2821 aacgagattc tccatacgga acctattcca caggaaggcc cttccccttt ccctgaccat
     2881 gttcttcatc atgatcgctt acggggcaat aattgtcttc gtcgggatct acgccggaca
     2941 gaggggtttc aggaacgtca ccgccttttt cctgtgcttc tccgcgtcca tcttcttttc
     3001 caggctcttc cttggcagac tcttcgacag ggggtatttt catcatctcg tcgttgccgg
     3061 cctcacattg accgcggccg ggctgccgtg gctcggcctg gctgcaaccc cgctccagtt
     3121 cctgggcgcc ggcatggtga gcggcttcgg tttcgggata ctgatgccga cgtgccaggc
     3181 agcggtcaac gatctcgcgg acgtcgatga aaggggcgcg gcaaattcca cgtatctcgt
     3241 ttcctacgat cttggagcgg gagtcggtgt ccttcttatc ggctttcttg cggagaggat
     3301 ccctcttggc aggatctacg cgtgctgctt cttcccgatc ctcctgtcgg cgggcatatt
     3361 cacgttcgcg gcccttcccc attatctccg caacagatcc ggcaaccgcg gcgccggctg
     3421 atcaaccgtc ggcggcacat caaccctttc caccgtgcct cgcgatctcc ttcatggcct
     3481 cgagcgtcag gtctatgtcg ctgccggtat tgaaaggccc gacactgaaa cgcacactgc
     3541 cgcccggaga cgtgtccaga ctctcatgaa cgagaggggc acaatgaact cccgttctga
     3601 ccgcgatgtt gaagtcagcg tccaggatgg cccccacatc gtgcgggtcc atgccccgaa
     3661 cgtttgccgt cagcaacgcg acgtggtcgg aaaggtcttc ggcgcaataa aggtctattc
     3721 ccgtgatcag agacaacccg tcgcggagtt tcgtgacgag ttccatctcc cgggcgtgca
     3781 ccgcctcgat acccgcctcc ccaagataat caaggctccc ggaaaggccg atgatgccga
     3841 gcaggttgag ggtgcccgcc tccaggcggt agggaaagtc gcgggtgtgg acggggctcg
     3901 ccgaatcgat ccccgtcccg ccaaagcggg aaggttctat gtcctccgtc tgcccggcga
     3961 ggacaatgcc gccgatcccc gtcggagcca gcatggactt gtgtcccgtg aaggcaaggg
     4021 ccgcgacctg ccacccgtcc acatcgatgg gaaccgcgcc ggcgctctgg gcggcatcga
     4081 cgaggagcgg cacaccgtgc tcggcgcaac gccgccctat ttcccggatc ggctggacgg
     4141 tgcccagaac gttggacgcg tgggtcacga caacgagctt cgtgtcgggc cggatggccg
     4201 ctatgatgtc cgcgggatcg acaaagcctt ttccatcgaa ggggacaaga tcgtattcga
     4261 tgatcctctt ctgacgcagg tggtaaaggg gccgaaggac ggagttgtgc tcgagcctcg
     4321 tggacacgac atggtcaccg gggtgaagca tgccctgaat ggctatgttc agcgcgtccg
     4381 ttgcgttacc cgtgaagatg acccggtcgg gagcggaaga gccgaagaag cgggcggcct
     4441 tgcgccgcgt ttccctgacg aactcctcgg cctcagcggc aaggtcgtag cctccccggc
     4501 ccggggagac accgatccga cgataggtct ccatcgcctt ctcgaggacc gagacgggct
     4561 tgggaaatgt tgtggcggca ttgtcgagat agaccggtcc tgcgggcata tcgttcacgt
     4621 gcatgacctc gggttcggca acccggcgat ccgccgcgct cccttgcgga taccgccggg
     4681 gaaaagcgcc tcgaggtcca tgagactcat cccgagaccc gccttgaggt cgcggttcat
     4741 cggcgccctg ccatgatagg cataatactc ccgcaggaaa cggatgaccc tccactggtc
     4801 gtcatcgagc ccttccatgc ctgtttccga tgcgagcata cgtcccacct cctccgtcca
     4861 atcctccggc cgccacagaa agccctcatc atcgaaaacg acctcctttc ccgcaatgac
     4921 cctcaccctc tgttgaagat cccatgttct ttcttcaggt tcagacaaac catccatcaa
     4981 ggcatgctca ctttcagaat ctcgtaaacc ataagggaga tcgtcatcca gccggggttt
     5041 tcgcccatat accgtcctcg ttgactcgat cagggatcca gcgccttttc taccccaacg
     5101 cctctcctgg atgtcgcttc cgcgagtgaa gacaccggcc ttcgccggaa tgacggcaac
     5161 gaagaacgct ccaacggctc cacccgctcg gagcactctg ttgtctttgt ctccaccctc
     5221 aagcgattca agccactcaa gcgattcaag ccgtcttttc gtccaacgcc gtccttattt
     5281 tttccggtgt cgccggcagg tcgtagatcc tcacaccaca ggcgtcgcag atggcgttga
     5341 tgacggcggg ggccgtgcac accatggtca tttcgccgat ccccaccgcc cccagggggc
     5401 ccttcgcccg aggggtctcc ctgattatca cgtccatgtc gaaggccgtc ttcatcgtgg
     5461 ggaacttgaa ggtgaaccag tccttcgtct cgccatggat gtactgctcc ctcagggcaa
     5521 agccgacacc ctggtccatc cccccgtgga gctggccctc gaggttctgg ggattgatga
     5581 cagtgccggg gtcgacggcg gtggtcatcc tgagaacgcg gacctcgccc gtatcggtgt
     5641 cgacctccac ctcggccatc tggatggcgt ggacacggga ttcgaaagac agcccctggc
     5701 ccgtttcggg gtccatgttg gcgtccttgc cggcagccat cttggaaccc aggtagtggg
     5761 tcggcttgcc cgccttcttc atgccttcat aggtatcgac gcctgcctcc tccatggcct
     5821 tcttcagctg ctcgatggcc agcaccagcg agccgccggc catgtaggtc atcctgctcg
     5881 ccgccgcggg gcccatcccc gtggtcctgt cggtgtcgcg ggtgacgagg cggatcctgt
     5941 ccatgggtat gcccgtgagg tgcgaggcta tctgggtgag catggaatcg ttgccttctc
     6001 ccgggtccgc gacggccgcg aagaccgtga ggccatcatc ggggtcgagc tccaccacca
     6061 cccttgctat gtccgcgggg gtcccgatgc cgaaggcgtg gctggctatg cccaccccgc
     6121 gccttacggg acccggcctc agggaggccg cttccctctt cgccctctcg taggccggcc
     6181 tgatggcgtc acagagctcc ggaaagggcc actcgtcgta caccattccc gtggagcagc
     6241 tttgcccggg cagaagggaa ttgatccgcc ggaactcgag ggcatccatg cccatcttcg
     6301 cggcgagcat gtccatggcg gactcgaggg cgaaggtgac ctggggaggc ccggctccac
     6361 gcgctgcccc gcccgcggga ttgttggtat agacaagacg cgccatggcg ttgacgttgg
     6421 ggatgctgta cgagcccgag agcatccaca gcgcacgttt cacgacggaa atgccgatta
     6481 tctggtacgc cccgttgtcc acggtgagat cgatcgtgaa ggccgtcagc ttcccatcct
     6541 tcccgcagcc gagcctcacc ttcatcgcga aggcatggcg cttgctgctc atgagcatcg
     6601 attccgcgag gctggggatg tagcgcacgg ggcggcggaa acgcagggcg gcggcgcccg
     6661 agatggcctc cgaggtcatc gcgatcttct gcccgaaatt gccccccgag aaggcctcct
     6721 cgtagcgcac gttctcgtac ccgaggctct cctgcaggtc cgccatgtgg aggtggatgt
     6781 tgatgctcct ccctatgacc acgagctggg catcatcgcc atcgccttcc atgtaggcca
     6841 ccgtcgcctc gggctcgagg ggggcctgat gattgatctg tgtcgagaaa tccgcctcca
     6901 ccagggcggc ggcctcgcga agcgcctttt cggcatcacc ctttatctgg ggctgtgtga
     6961 agcaaaggtt cggcgattcg gcgtggaccc tgacggcatc ctctttcatc gcctccaccg
     7021 gtgacgtgac aacgggcagg ggatcatact cgaccctcac ggccgccgcg gcgtcgagcg
     7081 cctccttctt cgttcgtgcc gccacgaccg ctatggcgtc tcccaggcag tagatcttgt
     7141 cttccgccag gatgggctgg tcggcggaga tgatcttgat cctgttcgtg cccttgatat
     7201 ccggggcggt catgatcccg accacccccg gcatcgtcgc ggcctcagac gtatccaccg
     7261 acaggatcct g
//
LOCUS       DAOPOA010000022         7241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_11437_length_7241_cov_4.049402, whole genome shotgun sequence.
ACCESSION   DAOPOA010000022 DAOPOA010000000
VERSION     DAOPOA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11437_length_7241_cov_4.049402"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..417)
                     /locus_tag="PKJ17_00890"
     CDS             complement(166..417)
                     /locus_tag="PKJ17_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74549.1"
                     /translation="MKTSPFDPEPVEVIAVSGRPVQVTFRKKRQKVRDIVNIWRVDDT
                     WWQRPVVRMYYTLELESGRRVTVFQDLPGGAWYRQNWTI"
     gene            complement(414..1583)
                     /locus_tag="PKJ17_00895"
     CDS             complement(414..1583)
                     /locus_tag="PKJ17_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74550.1"
                     /translation="MPHFHVQVECLKDPDLGKRPVVIVGMPEERGFVIDCSEELAHRG
                     VAPMMPLRDVYHLCYDAVPVLARRREHSLLWEEVLSSIAGITLRMEPKKEGTVFLDVT
                     RLPGMYKSEEQVASALGCMISDRFQLDVRIGAGNSRFIAFEAALCASKDARVIPSGME
                     RDFLSPLNIDHLPVPDDVRERLHLLGLHSLGQISAFTLSALTSQFGAAGKELWELSNG
                     VGERGRISCAFAVTDVDRELVCDGPVHSKQQIRAALLELLDGLCLELEELGKACRAIK
                     LVLDLQNRTFFEKQFVLHVPTTRKEDMLRRIMAGLEGVELASPVRIMSVRAGALVNHD
                     GRQERLFRSRPDLAKGMKDVSGFLKTKYGALSLARAVRNDSGTHLPDDMFIFVEP"
     gene            1823..2455
                     /gene="lexA"
                     /locus_tag="PKJ17_00900"
     CDS             1823..2455
                     /gene="lexA"
                     /locus_tag="PKJ17_00900"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOD74551.1"
                     /translation="MPRKTDTRENILDFIRTFRRENGYSPSVREVARHCGIKSPSVVQ
                     YHLDQLEQTGLITRDRDRSRSVGIVGEEGEVPQVPLLGVIAAGQPIGVPSADRWNSEA
                     QRMVDVPREVLKSRKDLFALEVRGNSMVDAMIADSDIVVMEQVDDVRNGDVAACWLER
                     EQEVTLKKVYFEKDKVRLQPCNPYMPPLYHDAANVRIQGRVVAVLRMNPR"
     gene            complement(2465..3217)
                     /locus_tag="PKJ17_00905"
     CDS             complement(2465..3217)
                     /locus_tag="PKJ17_00905"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOD74552.1"
                     /translation="MDGKKRESERHNHRNMSRSGRLIAQAVLFTAALLLLPGPAPMDG
                     AAQPAAEKPYFRKQPLPYPENALEPYISARTLSFHYEKHHQGYVNNLNKLVAGTPLAG
                     RSLEEVVRETAGRPDKTAVFNNAAQVWNHDFFWQCMKKGGGGKPAPGKLLRMIETSFG
                     SFERFANAFRTAAASLFGSGYTWLVQEGDALKIVQTSNADTPIAHGQTPLLTCDVWEH
                     SYYLDYQNRRPDFIGAFLDHLVNWEFVESRVR"
     gene            complement(3445..4341)
                     /locus_tag="PKJ17_00910"
     CDS             complement(3445..4341)
                     /locus_tag="PKJ17_00910"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOD74553.1"
                     /translation="MKRPVLHTIAVFIFLMLLLPFTSLARDIEPIVSTDWLQQHASDP
                     KLVIVDIRKVEEFKTGHIPNAVNVVYGSFAIMKAGLRNEMPHADDLSDVLSSAGIGAD
                     SHVVVVGNADTVPNRTDISRVAITLRYAGVTNVAILDGGYGKWLAEKKPVATDIVKPK
                     PVKHDIKANEAMFVKKDYVLGAIGKVTMIDTREPEFYEGKKKMDFVAKPGRIKGAVNL
                     PPSQAYTKDGTFKTRAELAALVAKAAGSDPAKEIILYCDTGKVCSSWGYVMTELLGYK
                     NVKIYDGSTEEWMKDPKAPVEP"
     gene            complement(4352..5371)
                     /locus_tag="PKJ17_00915"
     CDS             complement(4352..5371)
                     /locus_tag="PKJ17_00915"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD74554.1"
                     /translation="MDRKLLDSDPHFAEGCASCHRGDEKAASREKAHAGLVKRPSTDL
                     GICGKCHDKIAATYANALHFTTAGLRHGVSGRFSASERRVFDEKVFQQSCRSCHASCG
                     DCHVRSPRIAGVSTGLLKGHRFMAKDDGKTCAMCHGGRVYPEFTGDFGGSADIHYQKG
                     MTCLDCHGKSELHGTGTIQTSKREVKERPACEKCHKAGSEKTEKAKTSHAQHKDRVSC
                     YACHSGGVYTNCLDCHLGKGATPKPGFFLGLDPKNRKTVTTLRLVPTVRDTFKTAGIA
                     MEKYDSLPNYWATPTHNIKKRTDRTRSCEVCHVEKRDFLTKEGLIKNGSKANELLLYS
                     PKPLK"
     gene            complement(5423..5602)
                     /locus_tag="PKJ17_00920"
     CDS             complement(5423..5602)
                     /locus_tag="PKJ17_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74555.1"
                     /translation="MRNHSSGLVLLLSMLILTGFILPSLLYAAESGCVKCHTNERMLR
                     ALHQPVKVVLEEGVG"
     gene            complement(5744..6928)
                     /locus_tag="PKJ17_00925"
     CDS             complement(5744..6928)
                     /locus_tag="PKJ17_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD74556.1"
                     /translation="MLTKSFILELFSAAAMQRWNDKIRPVELKELDKQAHKMTIAYFL
                     GKFEESKEGFDWLEVIEGGLFEFLERLVITDLKPQIFNRIKEDRKRYEELTKWVYKRL
                     EPVISPLGDDFLERFRRYFSSTDNTINKRIINAAHFYATRWEFGIIERANPTGYEIPE
                     IRGFLQQRQERYYDLAGIQQLALFEKYRNFLDLCGQLRFQYRWSHINMIPRTSVLGHM
                     LIVAILSYLFSLQIGACPRRCFNNYFTGLFHDLPEVLTRDIISPVKRSIEGLDSLIKA
                     YEKEQMEKEVFNLIPPEWHPEMRTFTEDEFSSIALVDGKVFKTNTETISSRYNEERFN
                     PRDGAMIKAVDDLAAYVETYCSTENGVKSPELAEARLSISEKYRNAVIGGIHFPALFG
                     QF"
BASE COUNT         1441 a         1966 c         2164 g         1670 t
ORIGIN      
        1 ccgccttcga aaagatgcag gcgttgtaga gcccgtcgtg gtccgttatg gcaagggcgc
       61 ccatgccgag ggacgccgcc cgctcgacaa ggctccgggt cgaggatgcc ccgtcgagaa
      121 aggaaaagtt cgagtggaca tggagctcgg tgtacatggg gcccttcaga tagtccagtt
      181 ttgccggtac cacgcgccgc ccggcaggtc ctgaaagacg gtgacccgcc tgccgctctc
      241 cagctcgagg gtataataca tgcgaacgac cggcctttgc caccaggtgt cgtcgacacg
      301 ccagatgttg acgatgtctc tgaccttctg tctcttcttt ctgaaggtca cctgcaccgg
      361 ccggccgctg acggcgatca cttccacggg ttcgggatcg aagggggagg ttttcatggc
      421 tcgacaaaga tgaacatgtc atcgggcaga tgggtgccgc tgtcgttcct gacggcacgg
      481 gcgagggaca gggcgccgta ctttgtcttc agaaaaccgc tgacgtcctt catgcctttt
      541 gcgaggtccg gacgggaccg aaagagcctt tcctgccggc cgtcgtggtt tacgagggcg
      601 ccggcgcgta cgctcattat acggacgggg cttgcgagtt ccacgccttc cagcccggcc
      661 atgatcctgc gcagcatgtc ttccttgcgg gtcgtgggta cgtgcagaac gaactgtttc
      721 tcgaaaaagg tcctgttctg gaggtcgagt acgagcttta tggcgcggca cgccttgccc
      781 agttcttcaa gctcgaggca aagcccgtcg aggagctcga gaagggctgc cctgatctgc
      841 tgtttcgaat ggacgggtcc gtcgcagacc agttcacggt cgacgtcggt tacggcgaag
      901 gcgcaggata tcctaccgcg ctccccgacg ccgttggaga gctcccacaa ttctttcccg
      961 gcagcgccga actgggatgt cagggcggag agggtgaagg cgcttatctg ccccagggaa
     1021 tgaaggccaa ggagatgaag cctctcccgc acatcgtcgg gtacgggcaa gtgatcgata
     1081 ttaagggggg agagaaaatc cctttccatg cccgaaggta tgacgcgcgc atccttcgag
     1141 gcgcaaagcg cggcctcgaa ggcaatgaag cgggaattcc cggcgccgat cctcacgtcg
     1201 agctgaaacc ggtcggatat catgcatcca agggccgatg cgacctgctc ctcactcttg
     1261 tacatcccgg ggagccgtgt gacatcgaga aagacggtgc cctccttttt cggttccatg
     1321 cggagcgtta tcccggcaat ggaggagagg acctcttccc agagaaggct gtgctccctt
     1381 ctgcgcgcga ggacgggtac ggcgtcgtag cagagatggt atacatccct gaggggcatc
     1441 atgggagcaa cccccctgtg agcaagttcc tctgagcaat cgatgacaaa tcccctctcc
     1501 tcaggcatgc ccacgatgac gacgggtctc ttcccgaggt cggggtcctt caggcattcg
     1561 acctgaacat ggaaatgagg cacatgaaca caaaccactc gcataacaaa cagtatagaa
     1621 catatgttct agaaagacaa gggaaaagat aaagacggtt ttaagtttta cgttttaggt
     1681 tttaagtaaa aagaaacagc ttcattattt ttcgaatgga gtgccgcata tcttttgcct
     1741 ttacttaaaa cttaaaacct aaaacttaaa acttcttttt cttttcgaaa tatagtacac
     1801 ttgttctaac gggaggggca cgatgccgcg aaagacggat acgagggaga acatactcga
     1861 ctttatcagg accttccggc gggagaacgg ctattcaccg tccgtgcggg aggttgcgcg
     1921 ccattgcggg ataaagagcc cctccgtggt gcagtatcat ctcgatcagc ttgagcagac
     1981 gggtctcatc acgcgcgaca gggacaggtc gaggagcgtc ggtatcgtcg gtgaagaggg
     2041 agaggtcccc caggtgcctc ttctcggtgt catagccgcc ggccagccca taggggtgcc
     2101 ctcggcggac agatggaact cggaggcgca acggatggta gacgtgcccc gggaggtctt
     2161 gaagagcagg aaggacctct ttgccctcga ggtccgggga aactccatgg tggatgccat
     2221 gatcgccgat tctgacatcg tggtgatgga gcaggtggac gacgtaagga acggcgatgt
     2281 ggcggcatgc tggcttgaga gggagcagga ggtcaccctg aagaaggtct acttcgagaa
     2341 ggacaaggtg aggctccagc cctgcaaccc ctacatgcct ccgctgtatc acgacgcggc
     2401 caatgtccgc atccagggaa gggtggtggc ggtgctgcgt atgaacccga ggtaaacctt
     2461 ccctttatct caccctcgac tccacgaatt cccagttgac gaggtggtcg agaaaagccc
     2521 caatgaagtc gggacggcgg ttctggtaat cgagataata agaatgttcc cagacatcgc
     2581 aggtcaacag gggagtctgg ccatgcgcga tcggcgtatc ggcgttggat gtctgcacga
     2641 tcttcagtgc gtctccctcc tgaacgagcc acgtgtagcc gcttccgaag agggacgcgg
     2701 ccgcggtcct gaaagcgttt gcgaatcttt cgaaactgcc gaaagaggtc tcgatcatcc
     2761 ggaggagctt tccgggggcg ggtttgccgc ctcctccctt cttcatgcac tgccagaaga
     2821 agtcatggtt ccagacctgg gcggcattgt taaagaccgc ggtcttgtcc ggcctgcccg
     2881 cggtctccct gacgacctct tcgagagaac gtcccgcaag tggtgtcccg gcgaccagtt
     2941 tgtttagatt atttacgtac ccttgatggt gtttttcata gtggaagctc agcgtccggg
     3001 cggatatgta aggttccagc gcgttttcgg ggtagggtag gggctgtttc ctgaaatagg
     3061 gtttctccgc cgcgggttgt gcggcgccat ccatcggggc tgggccgggg agcagcagca
     3121 gtgctgccgt aaacagaacg gcctgcgcga tgagtcggcc cgaccgcgac atattccgat
     3181 gattatgcct ttcgctttct ctttttttcc cgtccatggc tttcctcctt attctttcca
     3241 tatgaagaga caccgggacc ggggccatta aagggagatg accgccgcct tcgtgccatc
     3301 cagaagtcta ccacaagggt cagggcggag agagcgcagt cagtccgtca tctgatgtca
     3361 ggggccgtgg aaggggtctt aaagggggtc aaaaaaaggc acccccgctt ccatcgggag
     3421 tgccttgaaa gaagatctca acgactaggg ttccacgggt gctttcgggt ccttcatcca
     3481 ttcttccgtt gaaccgtcgt agatcttgac gttcttgtag ccgagcaatt ccgtcatgac
     3541 gtacccccat gaagagcaca ccttacccgt atcgcaatag aggatgatct ctttcgcggg
     3601 atcggagccg gcggcctttg cgacgagggc ggccagttcg gccctggtct tgaatgtgcc
     3661 gtccttcgtg tatgcctgtg acggcggcag gttgacagca ccctttatcc tgcccggttt
     3721 cgccacgaaa tccatcttct ttttgccctc atagaattcg ggctcccgtg tgtcgatcat
     3781 tgtaaccttg ccgatagcgc ccagaacgta atccttcttc acgaacatgg cctcgtttgc
     3841 cttgatgtcg tgcttcacgg gtttcggttt gacgatgtcc gtggcgactg gcttcttctc
     3901 ggccagccac ttgccatagc ctccgtccag gatcgccacg tttgtgacac cggcgtagcg
     3961 gagcgttata gccacgcgtg atatgtcggt gcgattgggc acggtgtcgg catttcccac
     4021 gacgacgaca tgggaatcag ccccgatgcc ggcgctggag agaacgtccg agagatcgtc
     4081 cgcgtgcggc atctcattcc tcaatcccgc cttcatgatg gcgaaagagc cgtagaccac
     4141 gttgacggcg ttggggatat ggccggtctt gaactcttca accttcctga tgtcgacgat
     4201 gacgagtttc ggatcgctcg cgtgctgttg cagccagtcg gtggatacga tgggctcgat
     4261 gtcgcgggcg agtgacgtga agggaagcaa cagcatgaga aaaatgaaga cagcaatggt
     4321 gtgcagaacc ggtcttttca tggtctctcc tttattttag gggttttggg ctgtagagca
     4381 gaagctcgtt cgccttcgat ccgttcttga tgaggccctc tttcgtgagg aagtcccgtt
     4441 tctcgacatg gcacacctcg caggaacggg tccggtcggt cctcttcttg atgttatgtg
     4501 tgggcgtggc ccagtaattg gggagggagt cgtatttttc catcgctatc ccggcggtct
     4561 tgaaggtgtc gcggaccgtg gggacgaggc gcagggtcgt gaccgtcttc ctgttcttgg
     4621 ggtcgagacc caggaagaat ccgggtttgg gtgtggcccc tttccccagg tggcagtcga
     4681 ggcagttcgt atacacgcca cccgaatggc aggcatagca gcttaccctg tccttatgct
     4741 gtgcgtggga ggttttcgcc ttttccgtct tctcgcttcc tgccttgtga catttctcgc
     4801 aggcaggcct ttcttttacc tcccgcttcg atgtctggat cgttcccgta ccgtggagtt
     4861 cgctcttgcc gtgacagtcg aggcaggtca ttcccttctg gtaatggatg tcggcggatc
     4921 caccgaaatc tcccgtgaat tcgggataga cccgtcctcc gtggcacatg gcgcaggtct
     4981 tgccgtcgtc tttcgccata aatctgtggc ccttgaggag ccccgtgctc acacccgcta
     5041 tccgtggaga cctgacgtgg cagtcaccac aggaggcgtg gcaacttctg caggattgct
     5101 ggaagacctt ctcgtcgaat accctgcgct cactggcgga gaacctgccc gacacgccgt
     5161 gcctgagccc ggcagtggtg aagtgaaggg cgttggcgta tgtcgccgcg atcttatcat
     5221 ggcactttcc gcagatcccg agatctgttg acggtcgttt gaccaaaccg gcgtgagcct
     5281 tctcccggga agccgccttt tcatcgccgc ggtggcagga agcgcagcct tcggcaaaat
     5341 gggggtcgct gtcgagaagt tttctgtcca cgagaaaggc tttgtgccgg gactccgccg
     5401 ggaccgacgg aggcgctccc gctcacccca cgccttcctc gagcaccacc ttgacgggtt
     5461 ggtggagggc ccggagcatc ctttcattgg tgtggcattt cacgcagccg ctttcagcgg
     5521 cgtaaaggag tgagggcaga ataaaccccg tcagaatgag cattgataga agcaagacca
     5581 gacctgagga gtggtttctc atacggtgcc ttgtcaagaa tggtaattgt agggagggtt
     5641 tttgtcaagg gggggcgaaa aaaacggttc cagagttcaa aagttccagt gttcaagagg
     5701 gaaacacccc agacccttga acttttgaac ctttgaacgt tgtctagaac tgtccgaaaa
     5761 gcgcggggaa gtggatgccc cctatcactg cgttcctgta cttctcgctg atggacaggc
     5821 gggcttcggc cagttccggg gacttgacgc cgttctccgt ggaacagtat gtctcgacat
     5881 acgccgcgag gtcgtctacg gcttttatca tcgccccgtc ccttgggttg aaacgctctt
     5941 cattgtagcg gcttgatatc gtctcggtgt tggtcttgaa gaccttgccg tcgacgagtg
     6001 cgatgctgct gaactcgtcc tccgtaaagg ttctcatctc cgggtgccat tcgggcggga
     6061 tgaggttgaa gacctccttc tccatctgct ccttctcgta ggccttgatg agggagtcga
     6121 ggccttcgat ggagcgcttg acgggcgata tgatgtcccg ggtgaggacc tcaggcaggt
     6181 cgtggaagag gcccgtgaaa tagttgttga agcagcgcct gggacaggcg ccgatctgaa
     6241 gggagaagag ataggaaagg atggccacga tgagcatgtg gccgaggacc gatgtgcggg
     6301 gtatcatgtt gatgtggctc caccggtact ggaaacggag ctggccgcaa agatcaagga
     6361 agttccggta cttctcgaag agggcgagtt gctggatgcc ggcgagatcg tagtagcgct
     6421 cctgcctctg ctgaaggaag cccctgatct ccggtatctc atagcccgtc gggttggcgc
     6481 gctcgatgat gccgaattcc caccgcgtgg cgtagaaatg ggcggcgttg atgatccgct
     6541 tgttgatcgt gttgtcggtg cttgaaaagt agcgccggaa gcgttcaagg aagtcgtcgc
     6601 ccagagggga gatgacgggt tccaatctct tgtacaccca tttcgtcagt tcctcgtagc
     6661 gcttcctgtc ctcttttatc ctgttgaata tctgaggttt gaggtctgtt atgacgagcc
     6721 tttccaggaa ttcgaagagc cctccctcga tgacctccag ccaatcgaac ccttctttcg
     6781 actcctcgaa cttgccgaga aaataggcga tcgtcatctt gtgcgcctgc ttgtccagct
     6841 ccttgagctc cacgggccgt atcttgtcat tccaccgttg catggccgcg gcgctgaaca
     6901 attccagtat gaacgatttg gtgagcatca cagacttcat tgtaaaggaa tcgggaagaa
     6961 aaggaaaggg ggaaaggggg cgggatgcgg gacgcagggt cctgacaaga ccgataggac
     7021 gcttaaggac ctataggacg ggttgttgcc cgctgacggc ggcaactggc tgcagaacgt
     7081 tcctataagt cctatctgtc ctatacgtcc aatatgtcct ataggtccta tttttctatt
     7141 ggtcctatca gccccccctt tccactgatc cctctcctct gtccaataaa aaaggccgcc
     7201 cgcgggcggc cttttttatt ccatttctcg tttacgcctt t
//
LOCUS       DAOPOA010000023         6933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_12048_length_6933_cov_3.786857, whole genome shotgun sequence.
ACCESSION   DAOPOA010000023 DAOPOA010000000
VERSION     DAOPOA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12048_length_6933_cov_3.786857"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKJ17_00930"
     CDS             <1..298
                     /locus_tag="PKJ17_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74557.1"
                     /translation="VFQGRGHGAVSVEYRIPVESLKDEYADRARSLISSRKGNPSDSI
                     SLKHETYKQLVALSARAGKSIPDMVDEWARREWRFWLEGGDAVHPMAEANGKDT"
     gene            434..1636
                     /locus_tag="PKJ17_00935"
     CDS             434..1636
                     /locus_tag="PKJ17_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOD74558.1"
                     /translation="MKYLGNVKVTLLKERQEYRDLFSPLTESEYTDLRGSIQKGGIVN
                     DLIVEKSSDGYVVLSGHHRKAIAVELGIEQVPCSLAETPEEIVEALFDNASRRQMTEA
                     ERKAKIQEKEEIQDHFFEERLIPEIYDLYKQGKIDKESIDEFLDTSPEVQKSILLCLS
                     VERPVVPKEILQKHEQEIADIQGRFHESLAKERGKLQAAEAEKRKAEEALMKNSEELV
                     EIRERAEKTLAKYEKNKEQITKEVKKEVEKELEDLKKERETREAAIKAKQDKIAELNG
                     EIESFKNRIYGYESQVALWRHEIARVSELYNHSIAYYSNPALLEVQLQVISEYVESLV
                     RFSECHKWDGEAARTVERYQKSIDDLLRKLVQRVKANQKEAVSLEESEKTIAAQKDGN
                     LKAAPATS"
     gene            complement(1737..2102)
                     /locus_tag="PKJ17_00940"
     CDS             complement(1737..2102)
                     /locus_tag="PKJ17_00940"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOD74559.1"
                     /translation="MRFLVDNALSPLLAHGLKDAGHDAVHVREYGLQDADDQTIFDRA
                     ALEDRILLSADTDFGTILSNRGSDKPSVILFRRKSGRRPTAQLALLLQNLPPLQEALE
                     KGSVAVIEDNRIRIRSLPI"
     gene            complement(2099..2329)
                     /locus_tag="PKJ17_00945"
     CDS             complement(2099..2329)
                     /locus_tag="PKJ17_00945"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOD74560.1"
                     /translation="MKFKRITANPNQMGGMPCIRGLRIPVATIVGMVADGMTNAEILE
                     AYPDLQEDDIGEALRFAAEAVSERELPLVMAS"
     gene            complement(2418..2576)
                     /locus_tag="PKJ17_00950"
     CDS             complement(2418..2576)
                     /locus_tag="PKJ17_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74561.1"
                     /translation="MDKGKAAIWKIIAEMHEKMKDHDPQKIGKLIDEAVKETRKPWPV
                     KTTQERGR"
     gene            complement(2699..4114)
                     /locus_tag="PKJ17_00955"
     CDS             complement(2699..4114)
                     /locus_tag="PKJ17_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HOD74562.1"
                     /translation="MAQAGGFERLLEINRTGSCRPNNGVTLLGKGVNLADRSRDILLA
                     IPDAERSGHLGCFGTTRIGKTRLMESVIEQDVAKGYNLAIIDPKGDIDLFSKVIQVSA
                     EAGRLGEVMLLTPIYPDHSVKLDPLSHYYMEDELVDHVVSGIKAKEDYFIAIASEVTG
                     AVVSGLALKAKRFGRELNANFSDIKSRVTYKDLQDLKQELLLIKDALPEVIEVCDNID
                     QILSSTQEFFSKVASSLRTMLSALSSGNTGKIIGKSYVNEFVTRFEEGKGVILYCNTG
                     SLLARRTAHIIGRVLISMIQSMVGRFFASGRKLDPPLCIHIDEGHNIAYMGIQELFSK
                     AGGANTWISFYTQSIALIEQEIGPEAAKGIMDNINNWIFMRVNHPDTAQYMEDSSPIS
                     KKFQPIITPDECTGRITLREIEEQLILRAHVLQLADRQFYMRSRGSYYKAFTMESSPL
                     YVHVTFPELTGNDPAQSDQQL"
     gene            4340..4987
                     /locus_tag="PKJ17_00960"
     CDS             4340..4987
                     /locus_tag="PKJ17_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74563.1"
                     /translation="MVFSLKVGYLVPGVVVKSMPDHDSHLILISGTELLAFLPKKYAN
                     RSYKVGQNLVACVFMLENHKIILSQRSHHYYIRLAERIFSTLIEEEKIRIKRAVSVTG
                     AGFAKMAVEGLNGLDPVKECVPYLPAMKAYTDDTINLVRHSQDMKEYVRNALAPAPAD
                     KIRKVIFSRTLREAVVGVDPAYYGLFVGKGGANVATAAKLLDITILIRKAEDTNL"
     gene            4997..5620
                     /locus_tag="PKJ17_00965"
     CDS             4997..5620
                     /locus_tag="PKJ17_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74564.1"
                     /translation="MEDTAVEKSRADVIMERKLRLVKEQASIVLSPKMRDLHLLTQIL
                     FTTDKTINRMRMNAGTVRMPLFELEEANERITRFTSKVRAFTSALGGTNFYMSPGSSP
                     AEKEILARRRNAYVFMPKTSEGAALANIFISLDSAYCEFKIKSPLQDIRKLGEAIDTM
                     KEIVREFRDLTSDLAAKAQVRFVEPEGLAGYLKTMPDDERSVAGEEA"
     gene            5617..6282
                     /locus_tag="PKJ17_00970"
     CDS             5617..6282
                     /locus_tag="PKJ17_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD74565.1"
                     /translation="MRGPCLFLIGTLFIVIHTVESGATGIYQTDYSYPFEKMARSGLQ
                     AKTFMICEHAPASGPYLLRATRFPLPAVRVTQDVAPSPQGTKKEPEGPSREIAISQGI
                     EEKQSSPGQPVTILFTLDSAVLDDVAKAHLSSFVENMGARSKESYFSITGYTCDLGRK
                     DHNDILARQRAEAVAAYLRKAGIHLSQVTGTGKCCYATDDPGKRYLNRRVEVRAQKKE
                     VTQ"
     gene            6279..>6933
                     /locus_tag="PKJ17_00975"
     CDS             6279..>6933
                     /locus_tag="PKJ17_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOD74566.1"
                     /translation="MKTIVRSFVASLLTTTVLVSASFAGSSETEVRQVLSKTYPNIKV
                     GEIRSSDIPGLYEAEIGPNIVYFYPDKGLVVFGEIWTKDGKSLTAARREQLAAKKAGE
                     IPLEKGIRIGSGPNVVIEFTDPDCPYCRKASEYLKGRSDTTRYVFLLPLPAHKDAENK
                     ARFILCSNDKARTYEEVMSGKHDDRKVEPCGDEKVSVILREHRELAAKAGVSATPSFW
                     "
BASE COUNT         1705 a         1856 c         1957 g         1415 t
ORIGIN      
        1 ggtctttcag ggcaggggcc acggggctgt ctccgtcgag taccggattc cggtagagag
       61 cctgaaagat gaatacgcag accgggcgag aagcctgata tcgagcagaa agggcaaccc
      121 ttccgattcg atcagcctga agcatgaaac atacaaacaa ctggtggcgc tcagtgcgag
      181 ggccgggaaa agcattccgg acatggtcga tgagtgggct cgacgggaat ggaggttctg
      241 gctcgagggc ggggatgccg tccacccgat ggccgaggca aacggaaagg acacgtaagt
      301 gacgtgtaag gggtacacgt tccgacgtgc cattgtcacg ttccaacgtg tacgggggta
      361 cacgcatcga cgtgtcgccg gcacgttcca acgtgtacgg taatcacaat ataacccgaa
      421 gaggaggttt cccatgaagt atcttggcaa tgtcaaagtc actttactga aagagcggca
      481 ggagtacagg gatctgttca gtcccctgac ggaatccgag tacacggatc tccgtggaag
      541 tattcaaaaa gggggtatcg tgaacgacct gatcgtggag aaatccagcg acggctatgt
      601 cgttctctcc ggccatcatc gcaaggctat agcggtagaa ctgggaatcg aacaagttcc
      661 ctgttccctc gccgaaacgc cggaagaaat agtggaggcc cttttcgaca acgcgagccg
      721 ccgacaaatg acggaggctg agcgaaaggc gaagattcag gaaaaggagg agattcagga
      781 tcatttcttc gaggaacgtc tgattcccga gatttatgac ctttataaac aagggaagat
      841 cgacaaggag tctatcgacg agttcctcga cacgagcccc gaggtccaaa agagcatttt
      901 gctctgtctc tcggtggagc ggccggtcgt gccaaaagag attctgcaaa aacacgagca
      961 ggaaatagcc gacatccagg gccggttcca cgagagcctt gccaaagaac ggggaaaact
     1021 gcaagcggcg gaggcggaaa aacggaaggc ggaagaagcg ctcatgaaga acagcgaaga
     1081 gctggtggag ataagggagc gcgccgaaaa gacactcgct aagtacgaga agaacaaaga
     1141 gcagatcacc aaggaggtga aaaaggaggt cgaaaaagag cttgaggacc ttaagaagga
     1201 acgggaaacc agggaggcgg ctatcaaggc gaagcaggac aagatagccg agctgaacgg
     1261 agagatagaa tccttcaaga acaggatata cggctacgag tcccaagtgg cgctctggag
     1321 gcatgagatc gccagggtaa gcgagttgta caatcatagc atcgcctact attccaatcc
     1381 cgcgcttttg gaggtgcagc tccaggtgat ctcagagtat gtcgagtcat tggtgaggtt
     1441 ctcggaatgc cacaagtggg atggggaggc tgcaagaacc gtggaaaggt accagaagtc
     1501 catcgacgat cttcttcgga agcttgtcca acgggtaaaa gctaatcaga aagaggccgt
     1561 ctctcttgag gaatcggaga aaaccatcgc ggcccaaaaa gatgggaatc tcaaagccgc
     1621 accggcgact tcgtagcggg gcagcgcgaa ggagaaatga atattggggc aaacacgacg
     1681 cgaccgtcac cggtcgctgg tcggccaagc gtgaaagcag gttagttccg gatctctcaa
     1741 atgggcaagc ttcttatacg aatacggtta tcctcgataa ccgcaacact tcccttttcg
     1801 agcgcctctt ggaggggagg taggttttgc agtagaaggg ccagttgcgc agtcggtcgg
     1861 cggccggatt tacgtcggaa tagtattacg gaaggcttgt cgctgccgcg gtttgaaaga
     1921 atggtgccga aatcagtatc agccgataga agaatacggt cttccaacgc cgccctgtca
     1981 aaaatcgtct gatcgtcggc gtcctgcaac ccatattccc gcacgtgcac ggcgtcgtgg
     2041 ccggcatcct tcaggccgtg agcgagaagt ggcgacaacg cgttgtcgac gagaaacctc
     2101 acgacgccat taccaggggc aattctcttt cggacacagc ttcggcggca aacctgagcg
     2161 cctccccgat gtcgtcctct tgaaggtcgg gataagcttc aagtatctcc gcattggtca
     2221 ttccatccgc caccatcccc acaatcgttg ccaccgggat tctcaaaccc cggatgcacg
     2281 gcattccgcc catctggttc gggttcgccg ttatccgttt aaacttcatg tacgcctcct
     2341 attcaaacgc cgtatattct gcggacaatt cttccttgtt tcaaggcatc ccgcatcggc
     2401 atttgcatcc tgcatagtca tctgccccgt tcttgggttg ttttcacagg ccacggtttt
     2461 ctggtttcct tgaccgcttc gtcaatcaat ttgccgatct tctgcgggtc gtgatccttc
     2521 atcttctcgt gcatctcggc gattatcttc cagatcgcgg cctttccttt atccatttcc
     2581 acttcgcctg cggcaccctc ggttccaaca tcctcacctt ggctatgcta atcgccaacc
     2641 cagcgtatac attaatacta cgacacctgc ccgtgtacgt caaagaaaac caggcggatt
     2701 acagctgttg gtccgactgc gccgggtcat tgcctgtcag ttcggggaag gtaacgtgaa
     2761 catagagtgg gctggactcc atggtaaaag ccttgtagta actgcccctt gagcgcatgt
     2821 agaactgccg gtcggcgagc tgcagtacat gtgccctgag gataagctgc tcctctatct
     2881 ccctcaaggt gatcctgcct gtacattcat caggggtgat gatgggctgg aacttcttgc
     2941 ttatggggga tgaatcctcc atgtactgcg ccgtgtcagg atgattgacc cgcatgaaga
     3001 tccagttgtt gatgttgtcc atgatgcctt tggccgcctc aggccctatc tcctgttcga
     3061 tgagggcgat gctctgggta tagaaactga tccaggtgtt cgccccgcct gctttcgaga
     3121 agagttcctg aatgcccatg taggcgatgt tgtggccctc gtcgatgtgg atacagaggg
     3181 gtggatcgag cttcctgcct gacgcgaaga acctgcccac catggactgg atcatggaaa
     3241 tgagaactct tccgataata tgggccgttc gtcgggccag aagtgatccc gtgttgcagt
     3301 agaggatgac gccctttcct tcctcgaaac gggtgacaaa ctcgttcacg taagacttcc
     3361 caatgatctt ccccgtattg ccggaagaga gggcggagag catggttcgg agggatgaag
     3421 ccaccttgga gaagaactcc tgagtagacg agaggatctg gtcgatattg tcgcacacct
     3481 cgatgacctc gggaagcgcg tctttgatga ggagaagctc ctgtttgagg tcctgaagat
     3541 ccttgtacgt cacgcgggac ttgatgtcgc tgaagttggc gttcagctcg cggccgaacc
     3601 tcttcgcctt caaggccaga ccggaaacga ccgcgccggt tacctcgctc gcgatcgcga
     3661 tgaaatagtc ctccttcgcc ttgatgccgc ttaccacgtg atcgaccagt tcatcctcca
     3721 tgtagtagtg acttagtggg tcaagcttga cggagtgatc cggatagatg ggggtaagga
     3781 gcatgacctc tcccaacctg cccgcctccg ccgacacttg gatcactttg gagaaaaggt
     3841 caatgtcacc ctttgggtcg atgatggcca gattgtagcc tttggcaacg tcctgttcga
     3901 tcaccgactc catgagtctc gtcttgccga tgcgcgttgt cccgaaacaa ccgagatggc
     3961 cgctgcgctc cgcatcaggt atggcaagca agatgtccct ggatcggtcg gcgaggttca
     4021 ctcctttacc gagaagtgta accccattgt tcggacgaca actgcccgtc ctgttaatct
     4081 cgagcaatcg ttcaaatcca cctgcctggg ccatactcat tctcctcttc cagggtagta
     4141 cttccccgag cgatagacga agaagtaata ccgtatccgg tccgactgca attttcggtc
     4201 gctaaaagcg ggtcaggaag acgccggccc ttggactgca acctctcgtc gttgaggttc
     4261 ggccgcagtc cgtctcgtat gccggcgtca cagaaagacc ccggatcatt gtagtcggat
     4321 attcttccgg aggttgccca tggttttttc cctgaaagtg ggatacctgg tgcccggagt
     4381 ggtggtgaag tccatgcccg atcacgattc acatctcatt ctcatctccg gtacggagct
     4441 tctcgccttc ctgccgaaga agtatgccaa cagatcctac aaggtcgggc aaaacctcgt
     4501 ggcctgcgtc tttatgctgg agaatcacaa aataatcctt tcccaacggt ctcaccacta
     4561 ctacatacgc ctcgcagaac gcatcttcag caccctcatc gaggaggaga agatcaggat
     4621 caagagagcg gtctccgtca caggcgcggg attcgcgaag atggccgtgg agggtctgaa
     4681 cggtctcgat ccggtcaagg aatgcgtgcc ctatcttccg gcaatgaagg catacacgga
     4741 cgatacgatc aatctggtac gccattctca agatatgaag gagtatgtgc ggaacgccct
     4801 cgctcccgca ccggcggaca aaatccggaa ggtaatattc tcacgcacgt tacgggaggc
     4861 ggttgtcggg gtggacccgg cttactacgg actcttcgta gggaaaggag gcgcgaacgt
     4921 tgccacggcc gcaaagctcc tcgatatcac gatactcata aggaaggccg aagacacgaa
     4981 cctttaggag gcgaagatgg aagatacggc cgttgagaaa agcagggctg acgtgatcat
     5041 ggaaaggaag ctcaggctcg tcaaggaaca ggcgagcatc gtcctttccc cgaagatgcg
     5101 ggacctccat ctcctcaccc agatcctctt cacgacagac aaaacaatca acaggatgag
     5161 gatgaatgcg ggtaccgtac gcatgcccct tttcgaactg gaggaggcaa acgagaggat
     5221 cacgaggttc acgtccaagg tgcgggcctt caccagcgcc ctgggcggca cgaacttcta
     5281 catgtcaccc ggctccagtc cggccgagaa ggagatcttg gcgcgcagaa ggaatgccta
     5341 cgtcttcatg cccaagacct cggaaggggc agctctcgcc aacattttca taagcctcga
     5401 ttccgcatac tgcgagttca aaatcaagtc tccccttcag gatatcagga agctgggaga
     5461 ggccatcgat accatgaaag aaatcgtcag ggagttccgg gatcttacct cagacctggc
     5521 agccaaggcg caagtccgct ttgtcgagcc cgagggtctg gccggctacc tgaagaccat
     5581 gccggacgac gaaagatctg tggcagggga agaagcatga gagggccctg cctatttctc
     5641 atcggcaccc tgttcatcgt gatccacacg gtcgaatcgg gcgcgaccgg catctaccag
     5701 acggactaca gctacccatt cgagaaaatg gcgcgcagcg gcctgcaggc gaagacgttc
     5761 atgatctgtg aacacgcacc cgcttcagga ccgtatttat tgcgcgcgac cagattccct
     5821 ttgcctgccg taagagtcac ccaagacgtt gctcccagtc cccagggcac aaagaaggag
     5881 cccgaagggc caagcagaga gatcgccata agccaaggca tcgaggaaaa gcagagtagc
     5941 cccggccaac ctgtcaccat tctcttcacc ctcgatagcg cggttctcga tgatgtggca
     6001 aaggcccatt tgtcgtcgtt cgtcgaaaat atgggcgcaa gatcgaagga aagctacttt
     6061 tccataacgg gctatacctg cgatctgggg cgcaaggacc ataacgacat actggcccgg
     6121 caaagggctg aagcggtcgc agcctatctt cgaaaagccg gaatacacct ctcgcaagtg
     6181 acgggaaccg gcaagtgctg ttacgcgaca gacgacccgg gcaagcgata cctgaaccgc
     6241 agggtcgagg taagagcaca aaagaaggag gtcacccagt gaaaacaatc gttagatcat
     6301 tcgtagcatc actactcact acgaccgtac tggtatcggc gtctttcgcc gggagtagcg
     6361 aaacagaggt ccgccaagtc ctctcgaaga catatccgaa catcaaggtc ggcgagatca
     6421 gaagttccga tatccccgga ctctatgaag ccgaaatagg accgaatatc gtctacttct
     6481 atccggacaa gggtctcgtc gtgttcggag agatctggac aaaagacggg aagagcctga
     6541 ccgctgcaag aagagagcaa ctggcggcca agaaggctgg tgagatccct ctggagaaag
     6601 ggatcaggat aggcagcgga cccaacgtgg tgatcgaatt caccgatccc gattgcccgt
     6661 attgcaggaa ggcatccgag tacctgaaag gacggagcga caccaccagg tatgtctttc
     6721 tcctgcccct tccggcccac aaggatgccg agaacaaggc gcggttcatc ctgtgctcga
     6781 acgacaaggc aagaacatac gaggaggtga tgagcggaaa acatgacgac cggaaggtgg
     6841 aaccctgcgg cgacgagaag gtatcggtca ttctcagaga gcacagggaa ctcgcggcaa
     6901 aggccggggt gagtgccacc ccctccttct ggg
//
LOCUS       DAOPOA010000024         6905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_12113_length_6905_cov_3.634745, whole genome shotgun sequence.
ACCESSION   DAOPOA010000024 DAOPOA010000000
VERSION     DAOPOA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12113_length_6905_cov_3.634745"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PKJ17_00980"
     CDS             <1..376
                     /locus_tag="PKJ17_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOD74567.1"
                     /translation="NVGLNCIGPAVPVSRCVGRKRALELLLYGDLIDAQEALRIGLVN
                     RVVPTEELDERTTEWAARLAAKSPLAVETAKKAFYTGADMEYHKAFEHMTGTLARLCM
                     TQDVTEGISAFFEKREPQWKRR"
     gene            complement(419..841)
                     /locus_tag="PKJ17_00985"
     CDS             complement(419..841)
                     /locus_tag="PKJ17_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOD74568.1"
                     /translation="MPDQLDDFVKDLQDRIYDDALRDYGREAFDRWLDPKYMYVMDSA
                     DGHGRITGSCGDTMEVFLRFEGGRVREASFMTDGCGPSMICGSLAAELAMGKSPEEIR
                     QIGAGTILDTIGGLPEEDVHCASLAANALQVALHNCDK"
     gene            complement(844..1734)
                     /locus_tag="PKJ17_00990"
     CDS             complement(844..1734)
                     /locus_tag="PKJ17_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOD74569.1"
                     /translation="MRASIDCIPCFVRQTLEAARFVSDYPSVHEGVLREILRCLASLD
                     LDRPPPFVGQMIHRRLRELTGNPDPYLDAKNRHNRLALQLLPELKELVRKSEDPLKTS
                     VLLAITGNVIDLGARGDLTEDEVRSSMARTFSEPFHIDIEHLRREIGKASSILYLADN
                     AGEIVFDRLLIEELPVEQVTVAVRGAPVINDAVMDDAKAAGLHGLVRLVSNGSDAPGT
                     ILDDCSLEFQSCYKNADLVIAKGQGNYETLSSEEKNIFFLFKIKCGTVASHTGFKPGT
                     NVLTRGSRSRKQPEGSTIGE"
     gene            complement(1887..3248)
                     /locus_tag="PKJ17_00995"
     CDS             complement(1887..3248)
                     /locus_tag="PKJ17_00995"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD74570.1"
                     /translation="MKEKLAADERDVILDSINEGVFTVDLHWRITSFNQAAERITGVS
                     RQDSVGRLCSEVFRANVCENECSLRKTFETGKPVVNATAHIVNNKGFTVPIRISTAIL
                     KDREGNVIGGVETFQDLSQVEQLRKELQSRHTFEDIVGRSPAMMRLFEMLPLIAESSS
                     TVLIEGPSGTGKELFARAIHNLSPRRKKRFVAVNCAALPDTLLESEFFGHKAGAFTDA
                     RKDKPGRFALAQGGTIFLDEIGDISPALQVRLLRVLQERVIEPLGSTEPLKVDVRVIA
                     ATNKDLTNLVREGKFREDLYYRIRVVHLELPALKDRREDIPLLADHIIAKHNRLQGKN
                     IAGLSFEASTRLMGYDFPGNVRELENIIEQAFVLCGGDIIELHHLPPELRPALTSPAG
                     DSGLTNLRAMERHLIAETLRRYAGNRKRAARDLGIDGSTLYRKIRDLKIETPETDGRG
                     RRG"
     gene            3393..3851
                     /locus_tag="PKJ17_01000"
     CDS             3393..3851
                     /locus_tag="PKJ17_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOD74571.1"
                     /translation="MTGGTDVAIFICMVIAVTNWQGGVSPVFDVSDRLCVVETSDGAE
                     VRRATVLLKSRDPFGRAREVAAAGVEVLICGAVSHAFENALISSGILVLPFVCGDLEA
                     VIGAFLQGGLMDNRFLMPGCFGKRQNHGFRCRRGRRAHGGVLPTDINKRR"
     gene            3898..4173
                     /locus_tag="PKJ17_01005"
     CDS             3898..4173
                     /locus_tag="PKJ17_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74572.1"
                     /translation="MAKHRMICPFSGTACKECAVYRGRHYYLCYSAAYRGSSFDPERI
                     RELKEAETPDEPDKTFGMPEKIDVGARCMRNVEEYGVEREFAALKQN"
     gene            4507..5508
                     /locus_tag="PKJ17_01010"
     CDS             4507..5508
                     /locus_tag="PKJ17_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOD74573.1"
                     /translation="MDIFNTIVDLLQRGSHGVLATVVRRTGSAPRDVGAKMFVTNEGK
                     VFGTVGGGQLESEAYRKATEMMDQNVTVMLRVNMDAMRVEDRAMLCGGSVDILLEPVA
                     EEHRAVYEAVGKCMEDRERAVVLTRFGPRGFAKSLIREDGTVTGDHVDRQAVDRCVGL
                     ANLCQRELTEDAFAEPVRITVPLYIFGAGHVSQHLSKIAKAAGFSVTVIDDRKEFANI
                     ERFSEADAIVTGQFRDVFSSLDFTGNEYVAIVTRSQEQDALVLEEVLKRDARYVGLMG
                     SARKIAIIMGSLHEKGIGPEATGRVHAPIGIDIEAETPQEVAVSIVAELIRVKNAKR"
     gene            5495..6280
                     /locus_tag="PKJ17_01015"
     CDS             5495..6280
                     /locus_tag="PKJ17_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD74574.1"
                     /translation="MPNADLKYIYGPVPSWRLGSSLGIDLLSRPEKICSFDCTYCQLG
                     RNATKRTTRDRFVRTGDVIGEIESLPDVGIDYLTFSGRGEPTLASNLGEAIRALRKVR
                     KEKISVITNSSLMARKDVRRDLLGADFVIAKLDAATDDVFEAINRPAEGVCFRDVVEA
                     LADFRKEFPGRFALQVMFMKANKDAAREISDLAGEIGPDEVQLNTPLRPCMEQPLPGE
                     EMKRIKTFFNRQGLRSVDVYSAGKKAVVPLSGADTLRRRGKEI"
     gene            6289..>6905
                     /locus_tag="PKJ17_01020"
     CDS             6289..>6905
                     /locus_tag="PKJ17_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD74575.1"
                     /translation="MEIRVVFDNRKEDMHYFAGWGVSYLVNGHVLFDAGESEDMLFHN
                     MRSMNVGPEEIRKIVVSHEHWDHIGGLWRLLAHNPGMPVYVCPGVSGEFKDKIASFGG
                     GAITVEPFMEIDAGIYTTGETKGMSRYGIIPEQALVVRSKKGITVVTGCAHNGIAEIL
                     VSVRERMSEPIYLVLGGFHTLDLPPSTVRFLIKRFQEMGVGKVAPT"
BASE COUNT         1458 a         1847 c         2152 g         1448 t
ORIGIN      
        1 caatgtgggt ctcaattgca ttggacctgc ggtgcccgtc agccgctgtg tcgggagaaa
       61 gcgcgccctg gagctcttgc tgtacgggga cttaatagac gcccaggaag cgttgcggat
      121 aggacttgtg aaccgcgttg tccccaccga ggaacttgat gagagaacga cggaatgggc
      181 ggcacggctg gccgcaaaga gccccctggc ggtcgaaacc gcgaaaaagg ctttttatac
      241 gggcgctgac atggaatatc acaaggcctt cgagcacatg acgggtactc tggcccgcct
      301 ttgcatgaca caggatgtta ctgaagggat cagcgctttc tttgaaaagc gggagcctca
      361 gtggaagcgc cgctgaagac catcattgcc ccgaagatat gaggggacga gcgcccttct
      421 atttatcgca attgtgaagg gccacctgga gggcgttggc cgccagcgac gcgcaatgca
      481 catcttcctc tggaaggccc ccgatggtgt cgagtatggt cccggcgccg atctgcctga
      541 tctcctccgg gctttttccc atggcaagct ccgcggcgag agacccgcag atcatgctgg
      601 gcccgcagcc gtcggtcatg aaagaggctt cccggaccct tcctccctcg aaacgcagga
      661 agacctccat cgtgtcgccg cacgatccgg tgatacgtcc atggccgtcg gcgctatcca
      721 tcacgtacat gtatttcggg tcgagccacc tgtcgaaggc ctcccggccg tagtctctca
      781 gcgcgtcatc gtatatcctg tcctgcaaat ccttcacaaa gtcatcaagc tggtccggca
      841 tgtctattcc cctatggttg acccttcagg ttgttttcgc gaccgcgagc cgcgggtcag
      901 tacgttcgtt cccggcttga acccggtgtg tgaggccacg gtcccgcatt tgatcttaaa
      961 aagaaagaat atgttctttt cctcgctgct cagtgtttcg taattgccct ggcccttcgc
     1021 gatcaccagg tcggcatttt tatagcagct ctggaattcg aggctgcagt catccagtat
     1081 tgtccccggt gcgtcggagc cgttgctgac cagcctcacc aggccgtgaa gccccgccgc
     1141 cttcgcgtca tccatgacgg cgtcgtttat gaccggtgcc ccgcgaacgg cgacagtgac
     1201 ctgctcgacg ggcaattctt cgatgagaag gcggtcgaac acgatctcgc cggcattatc
     1261 ggcgagatac aggatactcg acgccttgcc tatctctctg cggagatgtt caatgtcgat
     1321 gtgaaaaggt tctgaaaacg tccgcgccat gcttgaacgc acctcgtcct ccgtgaggtc
     1381 accgcgcgcc ccgaggtcga tcacgtttcc cgtgatcgcg agaagaacgg acgtcttcag
     1441 ggggtcttcg ctcttgcgca ccaactcctt gagctccggc agcaactgga gggcgagacg
     1501 attgtgcctg ttcttggcat ccaggtaagg gtccggattt ccggtcagtt ccctgagcct
     1561 gcggtgtatc atctgtccca caaagggggg cggccggtcg agatcgaggc tggccaggca
     1621 ccggagtatc tctcgcagca caccttcgtg gacggaagga taatccgaca cgaaccgggc
     1681 ggcttccagt gtctgcctca caaaacaggg gatgcagtcg atggatgctc tcatattcca
     1741 ttacgaagca attcacgtgc catgcgttgc cccgggcgtg tgttacgtcg catcttcttg
     1801 atcagaaagg gtgtttagcc aaagaggatg ttacggcgtg attgcggaga agaagacagg
     1861 acggggcgcc catgcaatat gcaatactat cctcgccgcc ctcgaccatc ggtctcgggc
     1921 gtttcgatct tcaggtcccg tatctttcga tacagcgtgc tgccgtcgat gcccagatcg
     1981 cgggccgccc gtttcctgtt cccagcgtag cggcgcaggg tttccgcgat aagatgcctc
     2041 tccatggccc tgaggttcgt aagaccgctg tcgccagccg gggaggtgag agcgggccgg
     2101 agttccgggg ggaggtggtg gagctctatg atgtcaccgc cgcacagaac aaaggcctgt
     2161 tcgatgatgt tttccagctc gcgcacgttc ccgggaaaat cgtatcccat gagccgtgtc
     2221 gatgcctcaa aggacaggcc ggcgatattc tttccctgaa gacggttgtg cttggctatg
     2281 atatggtccg cgagaagagg gatgtcctct ctgcggtcct tcagggccgg gagttcgaga
     2341 tggacgacac ggatcctgta atagaggtct tcccggaact ttccttccct cacgagattt
     2401 gtcaggtcct tattcgtcgc ggcgatcacg cggacatcga ccttcaaagg ctccgtggac
     2461 ccgagaggtt cgatcacccg ttcctggagc acgcgcagca ggcgcacctg cagggccgga
     2521 gatatgtccc cgatctcgtc gaggaatatg gtgcctccct gggcaagcgc gaaacgtccc
     2581 ggtttgtcct tcctggcatc ggtgaaggcg ccggccttat gcccgaagaa ttcgctttca
     2641 agaagggtgt cgggcagggc agcgcaattc acggcaacaa agcgcttctt tcttctcggc
     2701 gaaagattgt gtatggcccg ggcgaagagc tcttttcccg ttccgctcgg gccctcgatc
     2761 aacaccgtgc tgctgctttc ggcaataagg ggaagcatct cgaaaaggcg catcatcgcg
     2821 gggctccttc ccacgatgtc ctcgaaggtg tggcggcttt gcagttcctt gcgcagctgc
     2881 tccacctggc tcaaatcctg aaacgtctcg acgccgccga tcacattgcc ttccctgtcc
     2941 ttcaggatgg ccgtggagat gcggatggga accgtgaaac ccttgttgtt cacgatgtgg
     3001 gctgtggcgt tcacgacagg ttttcccgtt tcgaaggtct ttctcagaga acactcgttc
     3061 tcgcagacgt tcgcgcggaa tacctcggaa caaagacggc ccacgctgtc ctgtctggaa
     3121 acccccgtaa tgcgctcggc ggcttgattg aaggaagtga tccgccaatg gaggtccact
     3181 gtgaaaacac cctcgtttat ggaatcaagg atgacgtccc gctcgtcagc ggcaagcttt
     3241 tctttcatgc cgctctcccg gcacgccgac acgtaatcgt gcttatcaca actataacca
     3301 tgaattagta ttgcataatg cctgagtgtt gtcaatcctt ggaatccgta ccgcggtctc
     3361 aggccttgtc acaactggat ccgtgacatt tcgtgaccgg cggtacggat gttgcaatct
     3421 tcatatgcat ggtcatagcg gtaacaaact ggcaaggagg tgtgtcaccc gtctttgatg
     3481 tgtccgatag attgtgcgtg gtcgagacga gtgatggtgc cgaggttcgg agggcgacgg
     3541 tattgctgaa atccagggat cccttcggaa gggccagaga ggtggcggcg gccggcgttg
     3601 aagtgttgat atgcggcgcc gtctcccacg cttttgagaa cgcgctgatc agctcgggaa
     3661 tcctggtcct gcctttcgta tgcggcgatc tggaagctgt catcggcgcg ttcctgcaag
     3721 gcgggttgat ggataaccgc ttcctcatgc cgggttgctt cggcaaacga caaaatcatg
     3781 gtttccggtg ccgccgcggg agacgtgcgc acggcggtgt gctgccgacg gacatcaata
     3841 agcggagata acgaatatgc caggcattcg taaaccttat caaagaaagg agccaatatg
     3901 gcaaagcaca gaatgatatg tccattttcc ggaacggctt gcaaggaatg cgcggtctat
     3961 cggggccggc actattacct ctgttattct gccgcgtaca ggggcagttc ttttgacccc
     4021 gagagaattc gggaattgaa agaagcggag acgccggatg aacccgacaa gaccttcggg
     4081 atgcccgaaa agatagatgt cggtgcccgg tgtatgcgaa acgtcgaaga gtatggtgtt
     4141 gagagggaat tcgcggccct caagcaaaac tgagctcgac cgaccgaccg tcgggttggt
     4201 gatcgaagag tgcggaatca aggagagcct caagggcggt aagctgttga aaggaatgaa
     4261 agaaaggagg taatgatcat gccaggtgga gatggaacag gacctcgtgg attcggtccg
     4321 atgacggggc gcgcggcagg tttctgcgcg ggcttgagcg ggagatggac cgtgtccgca
     4381 aacggatcgg tgagctcgaa aaggaaaaag gcggtaaggc aaagggctga gggagccggg
     4441 aacgctgggg agataccggg gcgtgcagtt cctttgttac aacgttgccc ccgtggaaag
     4501 ggaacaatgg atattttcaa cacgatagtg gatcttctgc agcggggttc ccatggcgtg
     4561 ctggcgacgg tggtgaggag aacgggatcg gccccgcgtg atgtgggcgc aaagatgttc
     4621 gtgacgaacg agggaaaggt gttcgggacc gtcggcggcg ggcagctgga atcggaggca
     4681 taccggaagg caacggagat gatggaccaa aatgtcaccg tgatgctccg tgtcaacatg
     4741 gacgcaatgc gggtggaaga tagggccatg ctctgcggag gcagcgtcga catccttctc
     4801 gagcctgtcg cggaagaaca tcgcgccgtc tatgaagcgg tcggtaagtg catggaggac
     4861 agggaacgcg cggtcgtcct tacacggttc ggtccccgcg ggttcgcaaa atcgttgatc
     4921 cgggaagatg gaaccgtgac cggcgatcat gtcgatcggc aagccgtcga tcgatgcgtg
     4981 ggtctggcga acctgtgcca gcgggaattg acggaggacg cattcgccga gccggtcagg
     5041 atcaccgtgc ctctatacat cttcggcgcc ggccacgtgt cccagcatct ctcgaagatc
     5101 gcgaaagccg ccggtttttc agtgaccgtc atcgacgaca ggaaggaatt cgcgaacatc
     5161 gagaggttca gcgaggcgga tgccattgtc acggggcaat tccgggacgt cttctcctct
     5221 ctcgacttca cggggaacga gtacgtggcc atagtgacga gaagccagga acaggacgct
     5281 ctcgtcctcg aagaggtcct gaagcgagac gcgagatacg tcggattgat ggggagcgcg
     5341 cgaaagatcg ccattatcat gggcagtctt cacgaaaagg gcattggccc ggaggcgacc
     5401 ggccgtgttc acgcaccgat agggatcgac atcgaggcgg agacaccaca ggaagtggcc
     5461 gtgagcatcg tggcggagct gataagggtg aagaatgcca aacgctgatc tcaagtatat
     5521 ctacggcccc gtgccatcct ggaggcttgg aagctccctc gggatcgatc tcctttcacg
     5581 tcccgagaag atctgctctt tcgattgcac ctattgtcag cttggcagga acgctacgaa
     5641 acggacgaca agggatcggt tcgtccgcac cggggatgtg atcggtgaga tcgaatcgct
     5701 gccggacgtg ggcattgact atttgacctt ttcgggcagg ggcgaaccca cgctcgcgtc
     5761 gaacctgggt gaggccatca gggccctgag gaaggtcagg aaggaaaaga tatcggtcat
     5821 aacgaactcg tccctcatgg ccaggaagga tgtgcgaaga gaccttctcg gcgcggactt
     5881 cgtgatagcg aagctggacg ccgccacaga cgatgtcttt gaggcgatca acaggcccgc
     5941 agaaggcgtg tgttttcggg acgtggtcga ggcccttgcc gatttcagga aagagttccc
     6001 gggtagattc gctctccagg taatgtttat gaaggcgaac aaagatgccg cgcgggagat
     6061 ctcggacctc gcgggagaga tcgggcccga cgaggtccag ctcaatacgc ccttgagacc
     6121 ctgcatggaa cagccgttgc ccggggagga gatgaagcgc atcaagacgt tcttcaacag
     6181 gcagggattg cgctcggtgg atgtttattc ggccgggaag aaggcggttg ttcccttaag
     6241 cggcgccgac acgttgcgaa ggagaggaaa ggagatataa tttcatagat ggagatcagg
     6301 gtagtcttcg ataacaggaa ggaagacatg cactacttcg cgggatgggg tgtctcctat
     6361 ctcgtcaacg gtcacgtcct tttcgatgcc ggggagagcg aagacatgct cttccacaat
     6421 atgcgatcga tgaacgtggg accggaggag atccggaaga tagtcgtgtc ccacgagcac
     6481 tgggaccaca taggcgggct gtggcgcctg ctggcacaca atccgggcat gcccgtgtac
     6541 gtttgtcccg gtgtgagcgg cgagttcaag gacaagatcg catccttcgg cgggggcgcc
     6601 atcaccgtcg aacctttcat ggagatagac gcgggcatct acacgacggg agagacgaaa
     6661 ggaatgtccc ggtacggcat aattcccgag caggcactgg tggtgagatc gaagaagggg
     6721 atcacggtcg tcaccggctg cgcgcataac gggatcgccg agatccttgt gtctgtccgt
     6781 gagcggatgt cagagccgat ctatctcgtt ctcggtggtt ttcatactct ggacctgccg
     6841 ccttccaccg tcaggtttct gataaaaagg ttccaggaga tgggagtggg caaggtggcg
     6901 ccgac
//
LOCUS       DAOPOA010000025         6876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_12173_length_6876_cov_3.569271, whole genome shotgun sequence.
ACCESSION   DAOPOA010000025 DAOPOA010000000
VERSION     DAOPOA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12173_length_6876_cov_3.569271"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PKJ17_01025"
     CDS             <1..598
                     /locus_tag="PKJ17_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOD74576.1"
                     /translation="EPFVAVIGGAVLAALAFAVSPQYLGLGLTTTENVLRGGDIIWYA
                     FLLKMIFTSVTFNSGGCGGIVTPVFFIGAAAGSLFAKIFGLDPATFAALGALSVMAGA
                     TNTPIAASIMALELFGTAIAPYATIACAMSFLMTGHRSVYPYQLLGVQKSHLLKVDLG
                     TEMENVHVEVDEKVEDLASRLLARLKDFRKVFSPDKEK"
     gene            714..1214
                     /locus_tag="PKJ17_01030"
     CDS             714..1214
                     /locus_tag="PKJ17_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74577.1"
                     /translation="MKTRTFLLVVIIAAVAAFVVLNWNSFLAPTTLSLGVTVVQAPLG
                     LVMLGLLVFLAALFLIFVVYLQTSFLIEARRNAKELQAKRELADKAEASRLTELRGYL
                     EAELKRQTDLDTESRAAVLTRLDQLDHDLRSAVEQTGNVLSAYLDELEDRLERHGLAS
                     RQDSSE"
     gene            1329..1586
                     /locus_tag="PKJ17_01035"
     CDS             1329..1586
                     /locus_tag="PKJ17_01035"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOD74578.1"
                     /translation="MGDNIDRVYVVSELVGTSTVSWEDAARKVVETASKTVKDLRIGE
                     VLKQDVTVENGKVVSYRVRLKVSFKFHREIAWYEEIRAGCC"
     gene            1723..3018
                     /locus_tag="PKJ17_01040"
     CDS             1723..3018
                     /locus_tag="PKJ17_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD74579.1"
                     /translation="MTRISLKRCLFALFAGFLMLCGLACSETPADHEKADDAVTIVFK
                     HGKIQGDAEHFRQLLLRFEKENPGIHVTGQALPSSTDEQHQFYVINLDAGSADFDVLS
                     MDVIWVPEFARAGWLRDLSALLPEAERRDIFPGPLKAVTSNGRIYAIPWYIDAGLLYY
                     RKDLLDGVGAKPPATWGELVRLAGEMTKGSEDIYGFIWQGKQYEGLVCNVLEYLWSHG
                     GDVLEDGKVVLNSPGNIAALTLMRDLIAKHRISPPLVTTLVEESSRHIFGNGRAVFMR
                     NWPYAWNIFQRNGSPVKGRIGVAPLPAAPGQPSASTLGGWQIGVNRNSRHPQAAERFV
                     RFITSRESQKTMALTIGYKPTRRSLYRDPDLIRQQPFITGLHDIFMNARPRPVSPYYM
                     MMTQILQPEFSAVISGIKTPAEALASADRQIRHVLGVER"
     gene            3015..3869
                     /locus_tag="PKJ17_01045"
     CDS             3015..3869
                     /locus_tag="PKJ17_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOD74580.1"
                     /translation="MRRVETGTWFVMPAILLLAAVTIFPIMDVVYLSLHRKLLIFGIS
                     EFTGLQNYRFLLQDERFWRALSNTIYFTGLSVVLEVVLGLALALLMNRAFRGKGLVRA
                     LVLVPWAIPTVVSAKMWEWIYNGDFGVLNYLLGTKVNWLGDPFWALHAAIIMDVWKTT
                     PFAAILLLAGLQLIPADLYSAARVDGAGPVRLFRYVTLPLLKPVILIVLIFRSLDAFR
                     VFDAVYVLTGGGPANTTETLSVYAYKVLFQTLQIGYGSALAVTVFVCVGLIATGYILL
                     MGRKAGLR"
     gene            3866..4678
                     /locus_tag="PKJ17_01050"
     CDS             3866..4678
                     /locus_tag="PKJ17_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOD74581.1"
                     /translation="MRKGMLYLAALVVVSYSVAPLVWQFLTSLKMDYELTRIPPLLPE
                     APSLAHYIAIFKGRMFLRIIANSALVASCTTILALAVGSLCSFGLAKLEIRYKALILG
                     FVLSISMFPPIATVSPLYLVIRAMSLRDTWLALVITYTTFSLPLSIWILTSFFRELPD
                     EIYRASRVDGCTVFQSFVRIVLPLAAPGFFATAILVFIFSWNEFLYALTFTSTEASRT
                     IPVAIALFPGLYEIPWGEIAAASVIVTAPLVVLVLVFQRRIIEGLTGGAVKG"
     gene            4683..5750
                     /locus_tag="PKJ17_01055"
     CDS             4683..5750
                     /locus_tag="PKJ17_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD74582.1"
                     /translation="MAAIRLENVQKMFGNVTVIRGMDLSIHDGEFFTFVGPSGCGKST
                     ILNVLAGLESVSSGAVYFDDVPVTDLSPRERDVAMVFQSYALYPHMTVYDNMAFPLRM
                     RKYERSRIEAEVTRAASMLGLAELLERKPRELSGGQRQRVALGRALVRSPKVFLMDEP
                     LSNLDARLRLETRGELKRLHKQLGTTTVYVTHDQAEALSLSDRIAVLDRGDIRQVGTP
                     REVYSDPANTFVAGFMGSPSMNFIAAVVRRREPLTVECNGLILSPAVKRTPEEEHVLI
                     GVRPEDISISKRVNGEGIAVSVLLVEPAGPFHWADVAWGPAVLRGMAEAQEELAPGDW
                     AHMAFHPDEALIFEAASGLRV"
     gene            5912..6823
                     /locus_tag="PKJ17_01060"
     CDS             5912..6823
                     /locus_tag="PKJ17_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOD74583.1"
                     /translation="MPQIPPLYLEILQKLAISIIIACLAVVVSRVAARLLRKRVKDGS
                     EMQTLGVLVRKITYITAVVIILVVVFELGSNFGTVLGILGAGVAFASQEVIGSFAGYL
                     NIITGSIFRIGDRIRIGNVVGDVLDISLLRTTVMEIGEWVKADQYTGRIVSLANRVVF
                     SDPVFNYTQYSRYLWDEIMVPVTYDTNWPRAVEIILAKGQELTLGFHAEARAEFEEMA
                     KKYPSLPAVPVEPAVYIAMTDNWVELTLRYIVEARERRSIKGQIHRELLTSFGEETEI
                     TVASMTVDIVKFPPLRSNDPAPKAPVP"
BASE COUNT         1367 a         1957 c         2067 g         1485 t
ORIGIN      
        1 tgagccgttc gtggcggtga taggaggcgc tgtccttgcg gctctcgcat tcgccgtatc
       61 tccccaatat ctcggtcttg gcctcacgac gacggagaat gtgctcagag ggggagatat
      121 catctggtac gccttcctgc tgaagatgat ctttacaagt gtgacattca acagtggcgg
      181 gtgcgggggc atagtgaccc ccgtattctt catcggcgcg gcggcgggat cgctgtttgc
      241 gaagatcttc ggactggatc cggcgacatt cgccgcgctt ggcgcgcttt cggtcatggc
      301 gggggcgaca aataccccca tagccgcgag catcatggcg ctggagcttt tcggaacagc
      361 catagcgccc tatgcgacga tcgcgtgcgc gatgagcttt ctgatgaccg ggcaccgcag
      421 tgtctaccca tatcagttgc tgggcgtgca gaaatcgcac ctgctcaagg tggaccttgg
      481 aactgagatg gagaatgtcc acgttgaggt cgacgagaag gtcgaagatc tcgcttcccg
      541 tttactggcc cgcctcaaag acttccggaa ggtgttctcc ccggacaaag agaaatgatg
      601 ataccggcga gccggcgagt ctgggcaagt ccggtgaaga ctgctacaat tatataaggt
      661 ggaacgcagg tagggacatc acgggaccga tcccttcaag gaggtttctt gccatgaaaa
      721 cacgcacgtt tttgcttgtg gtcatcattg cggcggtcgc cgctttcgtc gttctcaact
      781 ggaactcgtt ccttgcgccg acaacccttt ctctgggagt gaccgttgtt caggcgccgc
      841 tggggttggt catgcttggg cttctagttt tcctcgcagc gttgttcctc atattcgtgg
      901 tctacctgca gacctcgttt ctcatcgaag cccggcgaaa cgcgaaggaa ctgcaggcaa
      961 aacgggagct tgcggataag gccgaggcct cgcgcctgac tgaattgcgc gggtacctgg
     1021 aagcggaatt gaagagacag acggaccttg acaccgagtc gagggccgcg gtgctgacga
     1081 ggctggacca gctcgatcac gatctgcgtt ccgccgtgga acagacgggg aacgttcttt
     1141 ccgcgtacct cgatgaactg gaggaccgcc tggaaagaca cggcctggca tcgcggcagg
     1201 acagctccga atagtctgcc ccggcgttat ccggcacctg cgttctcttc acgccttctt
     1261 gacaggggcg ctatttgtgc tattaacacc gtatgaccgg aagcatcgaa ggttagaggg
     1321 aggtcaccgt gggagacaat atagacaggg tgtacgttgt cagcgagctc gtcggtacga
     1381 gcacggtatc ctgggaagac gcagcccgaa aggtcgtgga gaccgcttcg aagacggtca
     1441 aggaccttcg catcggcgag gtgttgaaac aggatgtgac ggttgaaaac gggaaggtgg
     1501 tgagctaccg ggtgaggctc aaggtgtcct tcaagttcca ccgggagatc gcctggtacg
     1561 aggaaatacg tgccggctgt tgttagccgg ggaatggctt ccgtagagta ttcctgtgcg
     1621 ggcgaaagac cttgcgggtc tattattctt gaaatgacag caggcctgta ggacaattgg
     1681 gagaagaaca gatcgcttcc cgacgttccg aagacacggc ccatgaccag gatatctctc
     1741 aaaagatgcc ttttcgccct cttcgcaggc ttcttgatgc tctgtggtct cgcctgcagc
     1801 gagacgcctg ccgatcatga aaaagcggat gatgctgtca ccatcgtttt caagcacgga
     1861 aagatacagg gcgatgcgga gcatttcagg cagcttctcc tcaggttcga aaaagagaac
     1921 ccgggcatac atgtgacggg ccaggctctt ccctcctcga cggacgagca gcatcagttc
     1981 tacgtcatca acctcgacgc gggttccgcc gatttcgacg tgctcagcat ggacgtcata
     2041 tgggtaccgg agttcgcgcg ggccgggtgg ctgcgcgacc tgagcgccct tcttcccgag
     2101 gccgagcggc gtgatatctt ccccggcccc ctgaaggcgg tgaccagcaa cgggaggata
     2161 tacgcgatcc cgtggtacat agacgcggga ctgctctact acaggaagga cctgctcgac
     2221 ggtgtggggg caaaaccccc ggctacctgg ggagagcttg tcaggctcgc cggcgagatg
     2281 acgaagggct cggaagacat ttacggattt atctggcagg gcaagcagta cgagggactt
     2341 gtctgcaatg ttctcgaata cctgtggagc catgggggcg acgtgctcga ggatgggaag
     2401 gtcgttctca acagccccgg gaatatcgcg gcgctcaccc tgatgagaga cctcatcgca
     2461 aagcaccgga tctcgcctcc gctcgtcacc actctagtgg aagagtcttc acggcacatt
     2521 tttggaaacg gcagggcggt cttcatgagg aactggccgt acgcgtggaa catctttcaa
     2581 aggaatgggt cccctgtgaa ggggaggatc ggcgtcgccc cgctgccggc ggcgcccggc
     2641 caaccatccg cttccaccct gggcggctgg cagataggag tgaacaggaa ctcgagacac
     2701 cctcaggcgg cagaacgttt tgtccgtttc atcacctccc gggaatcaca gaagaccatg
     2761 gccctgacga taggctataa gccgacgcgc aggtcgcttt accgcgaccc ggatctgata
     2821 aggcagcaac ccttcatcac cggccttcac gacatcttca tgaacgcacg gccccggccg
     2881 gtatcgccct attacatgat gatgacccag atcctccagc cggagttcag cgccgttatc
     2941 tccggcatca agacgcctgc cgaggccctg gcgtccgcgg acaggcagat acgacatgtt
     3001 ctcggagttg aacgatgagg cgtgtggaga ccggcacctg gttcgtcatg cccgccattc
     3061 tcctcctggc ggcggtcacc atctttccca tcatggatgt cgtttatctc agccttcacc
     3121 ggaagcttct catctttggc atatcagaat tcacggggtt gcagaactac cggttcctcc
     3181 tgcaggacga gcgtttctgg agggccctca gtaacacaat ctacttcacc gggctttccg
     3241 ttgtgctgga ggtcgtcctg gggcttgccc ttgcccttct gatgaacagg gctttcaggg
     3301 gaaaaggttt ggtgagggcg ctggtgctgg taccctgggc cattcccacc gttgtctcgg
     3361 caaagatgtg ggaatggata tataacggtg atttcggggt cctgaattat cttctcggga
     3421 caaaggtgaa ctggctcggg gaccccttct gggctctcca tgccgcgatc atcatggacg
     3481 tgtggaagac gacgcctttc gcggcgatac ttcttctcgc cggattgcag ctcatccccg
     3541 ccgatcttta cagcgcggcc cgcgttgacg gggccggccc ggtccgcctg ttccggtacg
     3601 tcactctccc gcttctcaaa ccggtgatcc tgatagtcct catcttccgc tccctcgatg
     3661 ccttccgggt ctttgacgcc gtgtacgtgc tcacgggcgg aggtcccgcc aacacgacgg
     3721 agaccctttc ggtctatgcc tacaaggtgc tcttccagac cctccagatc ggttacggct
     3781 cggccctggc cgtcacggtc ttcgtctgcg tgggcctcat cgcgacgggt tacattctgc
     3841 tcatgggcag gaaggcggga cttcgatgag aaaaggcatg ctctatctcg cggcacttgt
     3901 cgttgtttct tactcagttg caccgctggt atggcaattc ctgacgagcc tgaagatgga
     3961 ttacgaactg acgcggatac caccgcttct tcccgaagcg cccagcctgg cccattacat
     4021 cgccatattc aaagggagga tgttcctcag gatcatcgcg aacagcgcgc tcgtggcatc
     4081 gtgcaccacc attctggccc ttgccgtcgg ttccctgtgc tccttcgggc tcgccaagct
     4141 tgagatccgg tataaggccc ttattctcgg ttttgtcctc tccatatcca tgttcccgcc
     4201 catagccacc gtaagccccc tgtacctggt gatcagggcc atgagccttc gggatacatg
     4261 gctggccctt gtcattactt acaccacgtt ttcccttccc ctttcgatct ggatcctgac
     4321 gagcttcttc agggagcttc ccgatgagat atatcgcgcc tccagggtgg acgggtgcac
     4381 ggtctttcaa tccttcgtca ggatcgttct gcccctcgcg gcgccgggat tcttcgcaac
     4441 tgccatcctg gtgttcatct tttcatggaa cgagtttctc tacgcgctga ccttcacgtc
     4501 gacggaggcc tcccgaacca tccctgtcgc gatcgcgctc tttcccggtc tctacgagat
     4561 cccatggggg gaaatagccg ccgcgtccgt catagtgacc gcccctctgg ttgtgctcgt
     4621 tctcgtcttc cagaggagga tcatcgaagg gctcacggga ggggctgtta aagggtaagg
     4681 ccatggcagc gatacggctt gaaaatgtgc agaaaatgtt cgggaacgta acagtcataa
     4741 ggggcatgga cctttccatc cacgacgggg agttcttcac cttcgtggga ccgagcggat
     4801 gcgggaagtc gacgatattg aacgtgcttg cggggctcga gagtgtcagc agcggcgcgg
     4861 tctatttcga cgacgttccg gtgacggacc tttccccgcg ggagcgcgac gtggccatgg
     4921 tctttcagag ctacgcgctc tatccacaca tgaccgtcta cgacaacatg gcgttccccc
     4981 tccggatgcg gaaatacgaa cggtcccgga tagaagcgga ggtaacgcgg gccgcgtcga
     5041 tgctgggtct cgcggagctt cttgaacgaa aaccccggga gctttcgggt ggacagaggc
     5101 agcgcgtcgc cctggggaga gccctcgtca ggtcgccgaa ggtcttcctg atggatgagc
     5161 ccctctccaa tctcgatgcc cgcctgaggc tggaaaccag gggagagctg aagagactgc
     5221 acaagcagct gggaacgacg accgtctacg tcacgcatga ccaggccgag gcgctcagtc
     5281 tctcagacag gattgcggtg ctggaccgcg gcgacatccg ccaggttggg acgccccggg
     5341 aggtgtattc ggacccggcc aacaccttcg tggccgggtt catgggcagc ccttcgatga
     5401 acttcatcgc cgctgttgtg agacggaggg agccgctcac ggtcgagtgc aacggcctca
     5461 tcctgtctcc cgcggtgaaa aggacgccgg aagaggaaca tgtcctgatc ggcgtgaggc
     5521 cggaggacat ctccatatcg aaaagggtga atggtgaggg gatagcggtc agtgtcctgc
     5581 tcgtcgagcc cgccggtccc tttcactggg cggatgtggc atggggcccg gcggtcctga
     5641 ggggtatggc cgaggcgcag gaggagcttg cgccaggcga ctgggcacac atggcctttc
     5701 atcccgatga ggcccttatc ttcgaggcgg catcgggtct gagggtctga ggcggggatg
     5761 ccggttctca ccaggccctg tgtccgggac atatttccga taatatcttg caaattattt
     5821 gacattacag gagaattgaa aaggcgtcgg ctgaaagaga agccagccgg gcaaatggat
     5881 cgaaggattt gagacctcag ggaggttgat cgtgccgcag attccaccgc tctaccttga
     5941 gatactgcag aagctggcca tttcgatcat catcgcctgc ctcgccgttg tcgtcagccg
     6001 cgttgccgcg agactgctga gaaaacgcgt taaagacggc tctgaaatgc agacattggg
     6061 tgtgctcgtc cgcaagatca cctacattac ggccgtcgtg atcattctcg tggttgtctt
     6121 tgagctgggc agcaacttcg gcaccgttct gggtatcctg ggggccggcg tggcctttgc
     6181 ctcacaggag gtgataggct ccttcgctgg ctacctgaac ataatcaccg ggagcatatt
     6241 caggatcggc gacaggatac ggataggcaa tgtcgtcggt gacgttctcg acatcagcct
     6301 gttgcgaacg accgtcatgg agataggcga atgggtgaaa gctgaccagt acacgggcag
     6361 gatagtgagc ctcgccaacc gggtggtctt ctccgaccct gtcttcaact acacccagta
     6421 ttcgcgatac ctctgggacg agataatggt ccccgtcacc tatgatacga actggccccg
     6481 tgccgttgag atcatactcg cgaaggggca ggagctgacg ctggggttcc atgccgaagc
     6541 gagagcggaa ttcgaggaga tggcaaaaaa gtatccctca ctccccgccg ttcctgtcga
     6601 gccagccgtc tacattgcca tgacggacaa ctgggtcgag ctgacattgc gctacatcgt
     6661 tgaagcgagg gagcgcagat cgataaaagg acagatccac agagaactcc tcacgtcctt
     6721 tggcgaagag acggagatca ccgtggcctc tatgacggtt gatatcgtta agttcccacc
     6781 gttgagatcg aacgatcccg ccccgaaggc cccggtccct tgagacggat cggagcctcg
     6841 cgggttatcc atgcaccagt gtctatgcaa ggtctt
//
LOCUS       DAOPOA010000026         6836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_12266_length_6836_cov_3.243622, whole genome shotgun sequence.
ACCESSION   DAOPOA010000026 DAOPOA010000000
VERSION     DAOPOA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12266_length_6836_cov_3.243622"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKJ17_01065"
     CDS             <1..408
                     /locus_tag="PKJ17_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOD74584.1"
                     /translation="MLKRIAEALNVNIVDFFMKRANDDDVVTRKDSRIVIQLNRWDAR
                     IQSLVRNTADKKMQPFYTVIKPGGGSHGMYSHDGEEFGYVVQGEMDLMLDNKLHKIHK
                     DESFYFSSKVPHDWGNSGKEDVIVIWVITPPTF"
     gene            437..1927
                     /locus_tag="PKJ17_01070"
     CDS             437..1927
                     /locus_tag="PKJ17_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOD74585.1"
                     /translation="MPLLKEVSRNYGKLDLHINGKWVKPKTGKYFEATNPATGKVIAQ
                     APVAGPEDVEKAIVAAHAAFGKWRNVPFRDRAKLIFNLRDTFMKHHEELARILVQDHG
                     CTIEDGRGTISRCIENIESAGSSMYSFYKGEHVEQLANGIDCYLIHEPVGVFLIITPG
                     NIPMHAWSSFVPYALACGCTVIVKPSRQCPVAADAMSKMVDEAGFPPGVVNLLHMGPE
                     RELNRVILSDPRVQGVGLIGSTRVSKELFEICGQYGKRSSLNGNGKNTIVIMPDANLD
                     QSVNYILRGCFGMSGQRCLGSDNVVVLGDNKLYKTLKDKLIAASKAMKMGYGLDESVE
                     LGPMTTPGGREQVIGFVESGLKSGAKLLLDGRKAKVKGYEEGYFLGPNIFENVTPDMH
                     IAKEEAFGPLCNLLRMDSLDETIGFINSTNYGHSACIVTESGKSARKFIRECEVGNVG
                     VNAGIPQPYAFFGLGSKKDSFFGNSKSRMDSVKMFLDEKTVTLRWV"
     gene            2032..3114
                     /locus_tag="PKJ17_01075"
     CDS             2032..3114
                     /locus_tag="PKJ17_01075"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOD74586.1"
                     /translation="MCEECKKISRRTFLKGAAVVGAAAGLGVFDSKLLHAQSANLKFS
                     TWHPPVSREVKTVWTPMMEEVKKRSGGKLAYTMYAGAALGKGPEHYDIVAKGLSDMGY
                     FTATWTPGRFPLTDVLSLAVWVDGKDTAADIGNAVYHRVLKDEFKDVKVLELNGCIQS
                     FIWTKKPISKLSDLKGVKLRSPGGHQTNYIKSLGAEPVFMPLGDVYMGMETGTVDGIV
                     TCAPLVLAFKLAEVARYGTVLTFGCVSEGTVMNMKSWNRLPADQKKIVEDVCTNPFKV
                     TGGLGKDDYKKIMADIQKAGVKMIDLPKAEQELWYKRFQDVTRKWVSDMEAKKLNARR
                     AVAVMNEECEKRNVKLVSCPPEFKKV"
     gene            3136..3627
                     /locus_tag="PKJ17_01080"
     CDS             3136..3627
                     /locus_tag="PKJ17_01080"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOD74587.1"
                     /translation="MKALEGFQKGVHRVTYAVCAVGMFLAIPLMLITTFDVLGRAIFN
                     KPLPGTLELSEYMLSIIILLGAAYTQQVKGHVAVDFLTSRFPVKGQLVCKIITDILCL
                     FVITIMTVYGWIEGISETTVTDQLRVPMWPFKTLVAVCGLMLWAEFLTDLITSIMSLG
                     RRT"
     gene            3627..4934
                     /locus_tag="PKJ17_01085"
     CDS             3627..4934
                     /locus_tag="PKJ17_01085"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOD74588.1"
                     /translation="MDPVTVGILGCVLLVILLFLGMPIAFVMMFVGFTGLWALSSLDA
                     ALPKLAETVWGVANNYPYTIIPLFILMGSFAGSGGITRELYNTFDKWARRLPGGLGIA
                     TIAACAAFGAVSGSSVAAAAAMGNVALPEMRRFGYDPKLGCGVIAAGGTLSFLIPPSL
                     GFVVYGMLTEQSIGKLLISGILPGALMAAAFIACVVTLVGINPKLAPRSTEKVTFRDK
                     IIALKGVWEVLLVFCIVMGGIYLGFINPTEAGAIGATALFIIVIIKRKLTKENLFVAL
                     LEMARISIMVLFLVAGATLFSYFLALSTIPTAVSTWIAGLGVSKYVILVIIVAIYFLL
                     GCFLDSVSMMVLTLPVIFPVVVTIGFDPIWFGVLLVLMMEAGLITPPVGLNIYTIAGI
                     AKDVPMGTIFRGATPFLLSVLATAIILTLFPQIATYLPSLMGR"
     gene            4966..5445
                     /locus_tag="PKJ17_01090"
     CDS             4966..5445
                     /locus_tag="PKJ17_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOD74589.1"
                     /translation="MKTIHFTLNGKRTSVDVEDNETLLHALRERLGITSVKEGCGIGE
                     CGTCTVLVDDEPHYSCLTLASKANGRDIKTVEYLSSSGVLHPLQEAFVRSGAVQCGYC
                     TPGMLLSAYSLLLKNADPDAHDIKEAISGNLCRCTGYIQIEEAIKNAAEVCKCNSQG"
     gene            5408..6244
                     /locus_tag="PKJ17_01095"
     CDS             5408..6244
                     /locus_tag="PKJ17_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOD74590.1"
                     /translation="MLPKFVSVIARAKDEALSWLSSLENPKIIAGGTDLMVKMRAGKH
                     YSHVIDVAGVPELLGVTEKDGKLFIGPATTHARLSSEGSIVRNARSLALACGLVGSPQ
                     IRNMGTIGGNLANASPAADSIPPLLVHDAVVTLESRDASRKVPLEEAIVAPYVTSIDG
                     KELIGSIEISPLRGYREGYRRVAKRATWAISRLGVAFAIREENGMFLDAKLAIGSCTP
                     MPFRPRKVEDLLKGAPKNEATISDAIRMTLEEIKLISGIRPSYAYKLPVLKGILEEVL
                     RG"
     gene            6252..>6836
                     /locus_tag="PKJ17_01100"
     CDS             6252..>6836
                     /locus_tag="PKJ17_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD74591.1"
                     /translation="MFIGQDITRVDALDKVLGTAAFAHDLKKNNMLFACVVRSSRPHA
                     VIKRIDVSAARALEGVVTILSHEDIPGENLFGAIKKDQPFLAAGRVRYKGEAILVVVA
                     TTEEAARRAADLVSIEYEDLGNISDPFISIQSPVLIHDGGNLLSHRKVVKGDVAKGFE
                     SSDVIVENAYSTTWIDHAYMETEAGLGYLDDRGRV"
BASE COUNT         1536 a         2007 c         2004 g         1289 t
ORIGIN      
        1 atgctcaaga ggatcgccga ggccctgaac gtgaacatcg tcgatttctt catgaaaagg
       61 gccaacgacg atgatgtcgt gacccgaaaa gactcgcgca tcgtcattca attgaacaga
      121 tgggacgcca ggatacagtc cctcgtgagg aatacggccg acaagaagat gcagcctttc
      181 tacaccgtca tcaagcccgg cggcggctcc cacggcatgt attcacacga cggagaagag
      241 ttcggctacg tggtccaggg agaaatggac cttatgctgg ataacaaact gcacaagatc
      301 cataaggacg agagtttcta cttttcctcc aaggtccccc acgactgggg gaacagcgga
      361 aaggaagatg tcatcgtcat atgggtcata acgccaccta cgttctagga gtggccaatc
      421 tttcgaggag gaatcaatgc cattgctcaa ggaagtcagc agaaattatg ggaaactgga
      481 cctgcacata aatgggaagt gggtcaaacc aaagacaggc aagtatttcg aagccaccaa
      541 ccccgcgacg ggcaaggtga tcgcccaggc ccccgtggcg ggccctgaag atgtcgaaaa
      601 ggccatagtc gcggctcatg ccgcattcgg gaaatggagg aacgttccat ttcgtgaccg
      661 ggcaaagctt atcttcaacc tccgtgacac ctttatgaag caccacgagg aactggcaag
      721 gatcctcgta caggaccacg ggtgcaccat cgaagacggc aggggcacca tctcaaggtg
      781 catcgagaac atcgaatcgg cgggcagttc catgtacagc ttctacaagg gcgaacacgt
      841 cgagcagctt gccaacggga tagattgcta cctgatccat gagcccgtcg gtgtcttcct
      901 catcatcacc cccggcaaca tccccatgca cgcctggtcg tcattcgtcc cctacgccct
      961 cgcctgcggc tgcacggtca tcgtaaaacc gagccgccag tgcccggtcg ccgctgacgc
     1021 catgtccaag atggtcgacg aagcagggtt tccccccggt gtcgtcaacc ttctccacat
     1081 gggaccggag cgcgagctga acagggtcat cctctccgac cccagggtgc aaggggtggg
     1141 actcatcggc tcgacgcgtg tgagcaagga gcttttcgag atatgcggcc agtacggcaa
     1201 gcgttcgtca ctgaacggca acggcaagaa caccatcgtc atcatgcccg acgccaatct
     1261 tgaccagtcg gtcaattaca tcctcagggg ctgctttggc atgagcggcc agcgctgtct
     1321 cggctccgac aacgttgtgg tcctcggaga caacaagctc tacaagaccc tgaaggacaa
     1381 gctgatcgcc gcctcaaagg ccatgaagat gggctacggc ctcgacgaga gcgtggaact
     1441 cggaccgatg acaacgccgg gcggcagaga acaggtgatc ggcttcgtcg aatcgggcct
     1501 caagtcgggg gcgaagcttc tccttgacgg ccgcaaggcg aaggtgaaag gatatgaaga
     1561 gggatacttc ctcggcccca atatcttcga aaacgtcacc cccgatatgc acatagccaa
     1621 agaagaggcg ttcgggccgc tctgcaatct cctgagaatg gacagcctcg acgagaccat
     1681 cgggttcata aactcgacaa actacggcca cagcgcatgc atagtgacgg agagcggcaa
     1741 gtcggcgcgg aagttcatac gggaatgcga ggtcggcaat gtgggagtca acgccggcat
     1801 accccagccc tacgccttct tcggcctcgg ctccaagaag gattccttct tcggcaactc
     1861 aaaatcgagg atggattctg tgaaaatgtt ccttgacgaa aagacggtaa ccttaagatg
     1921 ggtttgatct tcacgggccc gacgcccggg ggtaggaata tcggtacgtt gtaaggtaaa
     1981 gcgaggtttc attgtcagcc acaggacaaa aacccaaaag gaggagatcc gatgtgcgaa
     2041 gagtgtaaga agatttcaag gagaactttt ctaaaggggg ccgcggtcgt cggggcggcg
     2101 gcagggttgg gcgtgttcga cagcaagctc ctgcacgcgc agagcgctaa tctcaagttc
     2161 agcacctggc acccgccggt gagccgtgag gtcaagacgg tctggacgcc gatgatggaa
     2221 gaggtgaaga agagaagcgg cgggaaactg gcctacacga tgtacgccgg cgccgcgctg
     2281 ggcaaaggcc ccgagcacta cgacatcgtg gcgaagggat tgtccgacat gggctacttc
     2341 acagccacct ggacaccggg acggttcccg ctgaccgacg tcctttccct cgcggtctgg
     2401 gtcgacggca aggacaccgc cgcggacatc ggcaacgcgg tctaccacag ggtgctcaag
     2461 gacgagttca aggatgtcaa ggtcctcgag ctcaacggct gcatccagtc ctttatctgg
     2521 acgaaaaaac ccatctcgaa gctctccgac ctcaagggcg tgaaactgag gtcgccgggt
     2581 gggcaccaga cgaactacat caagtctctc ggcgccgagc cggtcttcat gccgctcggt
     2641 gatgtgtaca tggggatgga gacgggcacc gtcgacggca tcgtcacctg cgcgccgctc
     2701 gtgctcgcgt tcaagctcgc cgaagtcgcg aggtacggca ccgtgctcac attcgggtgt
     2761 gtctccgaag gcaccgtcat gaacatgaaa tcctggaaca ggctgccggc cgaccagaaa
     2821 aagatcgtcg aagacgtctg cacgaacccc ttcaaggtga cgggtggtct cggcaaggat
     2881 gactacaaga agataatggc agacatccag aaggccggcg tcaagatgat cgacctcccc
     2941 aaagcggaac aggagctgtg gtacaagagg ttccaggacg tgacccgcaa atgggtttcc
     3001 gacatggagg caaagaaact gaacgccagg agggccgtcg cggtcatgaa cgaagagtgt
     3061 gaaaagagga acgtcaagct cgtttcctgt ccgcccgaat tcaagaaggt ctgatagaag
     3121 aggctacaga atgccatgaa agcgctggaa ggttttcaaa aaggggtgca cagggtgaca
     3181 tacgcggtat gtgccgtggg catgtttctt gccatccccc tcatgctgat caccactttt
     3241 gacgtgctcg ggagggccat cttcaacaag cccctcccgg gcacccttga actctcggaa
     3301 tacatgctgt ccatcatcat tctgctgggt gccgcgtaca cccagcaggt caagggccat
     3361 gtggccgttg acttccttac gtcgagattt cccgtgaaag ggcagctggt atgcaagatc
     3421 ataaccgaca tactgtgtct tttcgtcatt accatcatga cggtatacgg ctggatcgag
     3481 ggaatctccg aaacaacggt gacggaccag ctcagggtgc ccatgtggcc cttcaagaca
     3541 ctcgttgccg tgtgcgggct catgctctgg gcggagtttc tcaccgacct gataacaagt
     3601 attatgagcc tgggaaggag gacataatgg atcccgttac ggttggtata ttaggttgcg
     3661 tgctcctggt gatcctcctc tttctcggca tgcccatcgc gttcgtcatg atgttcgtgg
     3721 gtttcaccgg gctctgggcc ttgtcctcac tggacgcggc cctgccgaag ctggcggaaa
     3781 ccgtctgggg cgtcgccaac aactacccct acaccatcat cccccttttc atcctcatgg
     3841 gaagcttcgc gggctccggc gggataacga gggaactcta caacaccttt gacaagtggg
     3901 cccgcaggct gcccggcgga ctcgggatag cgaccatcgc cgcctgcgcc gcctttgggg
     3961 cggtgagcgg gtcttccgtc gccgccgccg ccgccatggg gaacgttgcc ctgcccgaga
     4021 tgcgaaggtt cggatacgac cccaagctcg gctgcggggt gatcgcggca ggcggcacct
     4081 taagctttct cataccaccc agcctggggt tcgtcgtcta cggcatgctt acggagcagt
     4141 ccatcggaaa gctccttata tcggggatac tgccgggggc tctcatggcc gcggcgttca
     4201 ttgcctgcgt ggtaacgctg gtaggcataa acccgaagct cgcgccgcgc agcaccgaaa
     4261 aggtgacctt cagggacaag atcatcgccc tcaagggcgt gtgggaagtc cttcttgtct
     4321 tctgcatcgt catgggcggc atctacctcg gcttcatcaa ccccacggag gcgggagcca
     4381 tcggcgccac ggcccttttc atcatcgtga tcatcaagag gaagctgacc aaagagaacc
     4441 tcttcgtcgc tcttctcgaa atggcgagga tatcgatcat ggtgctcttc ctcgtcgccg
     4501 gcgcgaccct tttcagctat tttctcgccc tgtcaacgat accgacggcg gtctcgacgt
     4561 ggatcgcggg gctcggcgtg tccaagtacg tcatcctcgt gatcatcgtc gcgatatact
     4621 tcctccttgg gtgttttctc gattccgttt ccatgatggt cctcaccttg cccgtcatct
     4681 ttccggtggt tgtgaccatc gggttcgatc ccatatggtt cggagtgctc ctggtgctca
     4741 tgatggaggc ggggctcatc acaccgccgg tggggctcaa tatatacact attgcgggta
     4801 ttgccaagga cgtgcccatg ggaaccatat tcaggggggc aacgcccttt ctcctgtccg
     4861 ttttggccac ggccatcatt cttaccctgt ttccgcagat agccacctat ctgccaagcc
     4921 tcatgggcag atgaacgagg tatgaaccac taaggggaag aagaaatgaa gaccatacac
     4981 ttcacgctca acggaaagcg gacctcggtc gacgtggaag acaacgagac cctgctccat
     5041 gctctcaggg aaaggctcgg catcacgagc gtcaaggagg gctgcggcat cggggaatgc
     5101 gggacatgca cggttctcgt cgacgacgag cctcattatt cgtgcctgac cctggcgtcg
     5161 aaggcgaacg gccgggacat caagaccgtg gaatacctct cctcgtcggg ggtgctccac
     5221 cccctgcagg aggccttcgt caggtcgggc gccgtccagt gcggctactg cacgccggga
     5281 atgctcctca gcgcttacag cctccttctc aagaatgcgg atcccgacgc ccacgacatc
     5341 aaggaggcca tcagcggcaa cctgtgccgg tgcacgggat acatacagat cgaagaggcc
     5401 ataaagaatg ctgccgaagt ttgtaagtgt aatagccagg gctaaggacg aggccctctc
     5461 ctggctctcc tccctggaga accccaagat catcgcgggg gggacggacc tcatggtcaa
     5521 gatgagggcg gggaaacatt acagccatgt catagacgtg gccggcgtcc ccgaactcct
     5581 cggtgtgacg gaaaaggacg ggaagctgtt cataggcccg gccacgacgc acgcgcggct
     5641 cagctccgag ggctccatcg tccggaacgc ccggagcctc gcgctggcat gcgggctcgt
     5701 cggctctccg caaatacgca acatgggcac catcgggggc aatctcgcga acgcctcccc
     5761 cgccgcggac tccatacctc ccctgctcgt gcacgacgcg gtcgtcaccc tggaatcgag
     5821 ggacgcgagc cggaaggtcc ctctcgaaga ggccatcgta gcgccttacg tcacatccat
     5881 agacgggaag gaactgatcg gctccataga gataagccct ctcagaggat accgggaggg
     5941 ttaccgccgg gtcgccaaga gggcgacctg ggcgatctcc cgcctcggtg tcgccttcgc
     6001 catcagggag gagaacggca tgttcctgga cgcgaaactg gctatcggct cgtgcacgcc
     6061 catgcccttc agacccagga aggtggaaga cctgctcaag ggggccccga agaacgaggc
     6121 caccatatcg gacgcgatac gtatgacatt ggaggagata aagctcatct cggggatcag
     6181 gccctcctat gcctacaagc tccccgtcct gaaaggcatt ctcgaagaag tgctcagagg
     6241 ttgaaggaac tatgttcata ggacaggata taacacgcgt tgacgccctc gacaaggttc
     6301 tggggacggc ggcctttgcc cacgacctca agaagaacaa catgctcttc gcctgcgttg
     6361 tccgcagctc ccgcccccac gcggtgataa agaggatcga cgtctccgcc gcccgtgccc
     6421 tggaaggggt cgtcacgatc ctctctcacg aggacatacc cggagagaac ctcttcggag
     6481 ccatcaagaa ggaccagccc ttcctcgccg cgggacgcgt ccgctacaag ggggaggcca
     6541 tcctcgtcgt cgtcgcgacc accgaggagg cggctcgcag ggcagcggac ctcgtcagta
     6601 tcgaatacga agacctcggg aacatatcgg atcctttcat ttccatacaa tcacccgtcc
     6661 tcatccatga cgggggaaac ctcctcagcc accgcaaggt cgtcaagggc gacgtagcga
     6721 agggtttcga atcctccgac gtcatcgtgg agaacgccta cagcacgacc tggatcgatc
     6781 acgcctacat ggagaccgag gcgggcctgg gctacctcga cgacaggggg agggtg
//
LOCUS       DAOPOA010000027         6753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_12447_length_6753_cov_3.420573, whole genome shotgun sequence.
ACCESSION   DAOPOA010000027 DAOPOA010000000
VERSION     DAOPOA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12447_length_6753_cov_3.420573"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PKJ17_01105"
     CDS             <1..789
                     /locus_tag="PKJ17_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOD74592.1"
                     /translation="ESIKFHCHGKWQQWGTGKELILPTLGMIGVDGALYSAMEYSGEA
                     IAALSMDSRFSIANMAIEAGGKNGIFPVDDKTLAYMKEHATRPYKVYASDADATYSEV
                     YEIDVSKVGFSVACPSLPSNVKDAGELGSIDVDQVVIGSCTNGRLEDLEMAAAILTGK
                     RVSKGLRCIVIPATPAIYGEAMRRGYFQTFLDAGCIISPPTCGPCLGGHMGILAKGER
                     AVATTNRNFIGRMGHPESEVYLAGPAVAAATAIKGKIARPEEVL"
     gene            786..1271
                     /locus_tag="PKJ17_01110"
     CDS             786..1271
                     /locus_tag="PKJ17_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOD74593.1"
                     /translation="MIVKGRIWKFGDNIDTDIIIPAQYLAHTDPKVLGQHCMEPLAPN
                     FPKEVRAGDIIVSGTNFGCGSSREHAPLAITALGIPLVVAKTFARIFYRNAFNKGLAL
                     LEADIYDLVKDGDEIEVDISTGIIRCGGKDVKAKPIPPFMVELINEGGLINHLKKKLE
                     G"
     gene            1272..2288
                     /locus_tag="PKJ17_01115"
     CDS             1272..2288
                     /locus_tag="PKJ17_01115"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOD74594.1"
                     /translation="MKQYNVAVAGATGAVGNEMVKVLEERNFPVKNLTLLASSRSVGK
                     TMSFHGEDVKVQELKEDSFKDIDIGLFSPGGSVSLKFAPIAAASGCVVIDNTSAFRME
                     KDIPLVVPEVNEHAIAQHTKRGIISNPNCSTIQMVVALKPLHDAAKIKRIVVSTYQAV
                     SGTGQKAIKELEQQVLAIYNMKTLEKKVYPHQIAFNVLPHIDSFLDSGYTKEEMKMVN
                     ETKKIMEDDSIAVTATTVRVPVFYGHSESVNIEFEREMTPDMARKLLKKAPGIKVVDD
                     PGKNKYPLAIDAAGKDDTYVGRIRVDDTVKYGLNMWIVADNIRKGAALNAVQIAEALI
                     KKYL"
     gene            complement(2372..5233)
                     /locus_tag="PKJ17_01120"
     CDS             complement(2372..5233)
                     /locus_tag="PKJ17_01120"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOD74595.1"
                     /translation="MFGFLRDLKLNIKISILGGGSVLITAAALVALAVWQSGQYNELA
                     QREVDRLLDADLDHIAQGAYNLVRTEDEAVKEQLSRNLNVMRHILTARGGADLSRQTV
                     EWTVVNQFTGQSVKTRLPRMLVGGKWIGQNSQPAVRTPVVDEVESLVGETATIFQRVN
                     ERGDMLRVATNVRDSEGRRAIGTYIPAVDDDGSPNPVIAAVLQGKTYHGRAFVVNAWY
                     LTAYEPLNDGAGNLVGMIYVGIKQKSVEMRIRHAIQQIKVGKTGYVYVLGGKGEDRGR
                     YIISWKGERDDENVWESTDSDGRYVIQEIIKKATVLKPGEMATERYRWQNPGETEPRW
                     KVARLAYYAPWDWVIGVGVHDDELHSYRAILSGGRNRMVRIMGVAGITITLFIGLLGI
                     FIAWTITRHIRQMTKAAEVIIAGDLNQTVDAASRDEVGTLARTFNLMTERLRETVAGL
                     RRSEEKYRSIFENSVEGIFRSTPQGRFLSINPAGVRMFGYGSQQEMIDSVVDIAQELY
                     VDPGDRERRKELLGSRGIVEGFEVEMRRKDGTRIWVSINSRVVRDAAGQVLYYDNTAE
                     DITERRRLEAQVRQSQKMEAIGTLAGGIAHDFNNILSVLMGYASLLQIELNEDDPLQE
                     YVEHIMASSQKAVNLTSSLLTFSRLQPVHLCPVDLNEAIRGTQKLLERLITDDIELKA
                     SLSTEDLVVMADTTQIDQILFNLASNARDAMPKGGTLTIETELVELDSDLAHAHGLGE
                     PGKYALLSVSDNGSGMDEHVKEHIFDPFFSTKETGKGTGLGLSTVYGIVKRHGGHINV
                     YSEKGMGTTFRIYLPAISGIAEQEIVSGQQVRGGTETVLVADDDADVRRLIRDVLTKY
                     GYRVLEAVDGEDAVEKFRQAGKVDLLVIDSVMPGKNGREAYEAISRITPGMKVIFISG
                     YTQDVVLEKGIEEGEFDFLSKPLGSNMLLRMVREVLDRE"
     gene            5417..6022
                     /locus_tag="PKJ17_01125"
     CDS             5417..6022
                     /locus_tag="PKJ17_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74596.1"
                     /translation="MPDIFAADGSGRELTFQVPLSEIYKFNEPEKAKELGQTLETGPQ
                     RPAAMAPAKRFIISTIGIFCLMIGLTGIFGQNPNIFSFLWIVLGGAIIWVFLAKPEMD
                     KKKAGTAPDTKKDPEVTLVVNAGRIVTRSPHNETARDWTEFAEYKKTKKGVRLSFLDG
                     TNIWLPENTFYDREEMRELLELLQKKVPGSRPQVSDEKPLE"
     gene            complement(6123..6455)
                     /locus_tag="PKJ17_01130"
     CDS             complement(6123..6455)
                     /locus_tag="PKJ17_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74597.1"
                     /translation="MALIDDIRKRTEEGLKTLKETAQDIAFSVEKQAMIGKRKYLDVA
                     KLQRSIQKVNAEIGEYVYDQFTCNKSVSSDDPFIRDRIGSITRMRLTIRDIEQEIAEL
                     ETTKPPKR"
BASE COUNT         1436 a         2102 c         1807 g         1408 t
ORIGIN      
        1 gagagcatca agttccactg ccacgggaaa tggcagcagt ggggcacggg caaggaactc
       61 atcctcccta ccctgggaat gataggggtt gacggcgcgc tttacagcgc catggaatac
      121 agcggtgagg ccatagccgc cctgtccatg gattcgcggt tctccattgc caacatggcc
      181 atcgaggccg gggggaagaa cggcatattt cccgtcgacg acaagacgct ggcctacatg
      241 aaggagcacg ccacgcggcc ctacaaggtc tacgcgagcg atgccgacgc gacatacagc
      301 gaggtgtacg agatcgatgt ctccaaggtc ggcttcagcg tcgcgtgccc ctcgctcccg
      361 tccaacgtga aggacgcggg ggagttgggc agcatagatg tggaccaggt cgtcatcggt
      421 tcctgcacca acggcagact cgaggacctg gagatggcgg ccgccatcct cacagggaag
      481 agggtcagca aaggtttgag atgcatcgtg atccccgcga cccccgcgat ctacggcgag
      541 gcaatgcgcc ggggatactt ccagacattc ctggatgccg gctgcatcat atcgccgccc
      601 acctgcggtc cctgcctggg cggtcacatg ggcatactgg caaagggaga gagggccgtg
      661 gccacaacga acaggaactt catcggaagg atggggcatc ccgaaagcga ggtctacctc
      721 gccggtcccg ccgtggcggc tgccaccgcc atcaagggca agatagcccg tcctgaggag
      781 gtattatgat cgtcaaagga aggatctgga aattcggcga taatatcgac accgatatca
      841 tcatccccgc gcaatatctc gcgcacacgg acccgaaggt cctgggacag cactgcatgg
      901 agcccctcgc cccgaacttt ccgaaggagg tcagggccgg ggacatcatc gtctcgggga
      961 ccaatttcgg ctgcggctca tcgcgggaac acgcgccgct cgccatcacc gcgctcggca
     1021 taccccttgt cgtcgcgaag acattcgcca ggatattcta ccgcaacgcc ttcaacaagg
     1081 gcctcgcgct cctggaggcc gacatctacg atctcgtaaa ggacggtgac gagatcgagg
     1141 tcgacatatc gacgggcatc atccgctgcg gcggaaaaga cgtgaaagcc aaacccatcc
     1201 cccccttcat ggtggaactc atcaacgagg gcgggttgat aaatcatctc aagaaaaagc
     1261 tggagggcta aatgaaacag tacaacgtag cagtcgccgg agccaccggc gcggtcggaa
     1321 atgaaatggt aaaagtcctc gaggaacgga atttcccggt caagaacctg acacttctcg
     1381 cttcctcgcg gagcgtgggc aagactatga gcttccacgg tgaagatgta aaggtacagg
     1441 agctgaagga agactccttc aaggacatcg acatcggcct gttctccccc ggtggatccg
     1501 tgagcctcaa gttcgcgccc atcgccgcag cgagcggctg tgtcgttatc gataacacca
     1561 gcgccttcag gatggagaag gacattcccc tcgtcgtgcc cgaggtgaac gagcatgcca
     1621 tcgcccagca cacgaagcgc ggcatcatct ccaatcccaa ctgctccacg atccagatgg
     1681 tcgtcgcctt gaagccgctc cacgacgcgg cgaagatcaa aaggatcgtc gtgtccacct
     1741 atcaggccgt ttcgggaaca ggtcagaaag ccataaagga actggagcag caggttctcg
     1801 ccatttacaa catgaagacc ctcgagaaaa aggtctatcc ccaccagatc gccttcaacg
     1861 tcctgccgca catcgacagt ttcctcgaca gtggctacac caaggaagag atgaagatgg
     1921 tcaacgagac gaagaagatc atggaagacg attccatcgc cgttacagca acaaccgtaa
     1981 gggtccccgt cttctacgga cactccgaat cggtgaatat cgagttcgaa cgggaaatga
     2041 cccccgacat ggcccgcaag ctgctgaaaa aggcccctgg tatcaaagtg gtcgacgacc
     2101 cgggcaagaa caagtatccc ctcgccatcg acgccgccgg caaggacgac acctacgtcg
     2161 gcaggatcag ggttgacgat accgtcaagt acggcctcaa catgtggata gtcgccgaca
     2221 acatcaggaa aggtgcagcc ctgaacgccg tccagatagc ggaagccctg ataaagaaat
     2281 atctgtaacc ttcagaccgg attgcgaaaa ccctccctcg aaaggggagg gttttttctt
     2341 tcacgccggg ccttgaacgc gggagacctc gtcattccct gtccagcacc tccctgacca
     2401 tcctgaggag catgttggac cccaggggct tcgagaggaa atcgaactct ccctcctcaa
     2461 tgcctttctc caggaccaca tcctgggtgt acccgcttat gaagatgacc ttcataccgg
     2521 gagtgatcct gcttatcgcc tcgtaggctt ccctcccgtt ctttccgggc atcaccgagt
     2581 cgatgaccag aaggtccacc ttcccggctt gcctgaactt ttccaccgcg tcttcaccgt
     2641 cgacggcttc gaggacccta tacccgtact tcgtcaacac gtcccgtatc agacgccgca
     2701 cgtcggcatc gtcgtcggca acgagaacgg tttcggtgcc ccctctcacc tgctgcccgg
     2761 acacgatctc ctgttccgcg atcccggaga tcgcgggaag gtatatgcgg aaggtcgtgc
     2821 ccatgccctt ctcgctgtac acattgatat gacctccatg ccgctttacg atcccgtaga
     2881 ccgtcgagag tcccagaccg gttcctttgc ctgtctcctt ggtagagaag aagggatcga
     2941 aaatgtgctc tttcacgtgt tcgtccatgc ccgatccgtt gtcggagacg gacaggaggg
     3001 cgtattttcc cggttcaccc agtccgtgag cgtgagcaag atcgctgtcc agttccacga
     3061 gttccgtctc gatcgtcagc gtcccgcctt tcggcatggc gtccctggca ttgctcgcaa
     3121 ggttgaagag tatctgatcg atctgggtgg tgtccgccat gacgaccagg tcttccgtgc
     3181 tgagagatgc cttgagctcg atatcgtcgg taatgagcct ttccagcaat ttctgggtgc
     3241 cccttatcgc ctcgttcagg tcgacaggac agaggtgtac cggctggagc ctgctgaagg
     3301 taagcagact cgatgtcagg ttcacggcct tctgtgaaga cgccatgatg tgctcaacgt
     3361 attcctgaag ggggtcgtcc tcgttgagct cgatctgcag gagcgacgcg taccccatca
     3421 ggaccgaaag aatgttgttg aaatcatggg cgataccgcc cgcgagggtg ccaatggcct
     3481 ccatcttctg cgactgccgg acctgggcct cgagacgcct gcgctcggtt atgtcctcgg
     3541 cggtattatc gtagtagagc acctgccccg cggcatcacg gaccactctg gagtttatgc
     3601 tcacccagat cctggtgccg tccttgcggc gcatttccac ctcgaaacct tccacgatac
     3661 cccgcgaccc aagcagctct tttcttcttt cccggtcccc gggatcgaca tacaactcct
     3721 gagctatgtc aacgacggaa tctatcatct cctgctgcga tccgtacccg aacatccgca
     3781 cacccgccgg attgatgctc aggaaccgcc cctgaggtgt cgaccggaat atcccctcca
     3841 cggagttctc gaagatgctc cggtatttct cctcgctccg acgcagtccc gccactgttt
     3901 ccctgagcct ttccgtcatc aagttgaatg tccgcgccag ggtgccgacc tcgtcgcggg
     3961 aggcggcatc cactgtctgg ttgagatcgc cggcaataat gacctcagcg gctttcgtca
     4021 tctgacggat gtggcgagtg atggtccagg cgatgaagat gcccaggagt cctatgaaca
     4081 gtgtgatcgt gatcccggcc acacccatga tgcgcaccat ccggttcctt ccgccgctca
     4141 ggattgcacg atacgagtgc agctcatcgt cgtgaacacc tacccctatc acccagtccc
     4201 agggagcgta ataggcgagg cgggccacct tccacctcgg ctccgtctcc cccgggttct
     4261 gccagcggta acgttccgtg gccatttccc cgggtttcag aaccgttgcc ttctttatga
     4321 tctcctggat cacgtagcgg ccgtcactgt ccgtgctctc ccagacgttt tcgtcgtccc
     4381 tctccccctt ccaggatatg atgtagcggc cccggtcctc tcccttgcct cccaggacgt
     4441 agacatatcc cgtctttccg accttgatct gctggatcgc atggcgtatc cgcatttcga
     4501 cgctcttctg tttgataccg acatatatca tccccaccag gttgcccgcc ccgtcgttga
     4561 gcggttcgta cgccgtcagg taccaggcgt tcaccacaaa ggcacggccg tgatacgtct
     4621 ttccctgcaa caccgccgcg atgacagggt tcgggctgcc gtcgtcatcg acagcgggta
     4681 tgtaggttcc tatggcacgc ctgccctctg aatccctgac gttcgtcgcc acgcggagca
     4741 tgtctccccg ctcgttcacg cgctgaaaga tggtggcagt ctcaccgaca aggctctcga
     4801 cctcgtcgac caccggggtt ctgacagcgg gctgcgaatt ctgcccgatc catttcccgc
     4861 caacgagcat ccgcggcaat ctcgtcttca cggactgtcc ggtgaactgg ttcaccactg
     4921 tccactccac ggtctgacgg gagaggtccg cgccgcccct ggcggtcagt atgtggcgca
     4981 tgacattgag gtttcttgac agctgctcct tgacggcctc atcctccgtc cggaccaggt
     5041 tgtaggcacc ctgggcgatg tggtccaggt ccgcatccag cagccggtcc acctctctct
     5101 gcgcgagctc gttgtattga ccgctctgcc agacggcgag cgccacaagg gcagccgccg
     5161 tgatgaggac gcttcctcct cccaggatgg atatcttgat gttgagcttc aggtccctaa
     5221 gaaaaccaaa catccgctcc ttcctaaccc tcacaacccc ccattaggca agtatagcac
     5281 cgaggccttc ttttgggtgt tccttatgcg gacatcgaaa ctgtcgccag gaaaacccct
     5341 ttcttcccat cacccatcac ccgcttttct tgcttttccc tttgcatttg aagccacaaa
     5401 cgcctagaat atccccatgc cagatatctt tgcagcggac ggatccgggc gggaactgac
     5461 attccaggtc cctctatccg agatatacaa gttcaacgaa ccggaaaagg ccaaggagct
     5521 tggccagaca ctggagaccg ggccgcagag gcctgccgcc atggctcccg ccaagaggtt
     5581 catcataagc acgataggga tcttctgcct catgatcggc ctaacgggca tcttcggcca
     5641 gaaccccaac attttcagct tcctctggat cgtcctcggc ggggcgatca tctgggtctt
     5701 tctggcaaag ccggagatgg acaagaagaa agccgggacc gccccggata cgaagaagga
     5761 ccccgaggtg accctggttg tcaacgccgg caggatcgtc acgcgctcgc cccacaacga
     5821 aacagcgagg gactggacgg aattcgctga gtacaagaaa acaaagaagg gggtccgcct
     5881 gagcttcctc gacggcacga acatctggct ccccgaaaac accttctatg acagggaaga
     5941 aatgagggaa ctcctggaac tgctgcaaaa aaaggtccca ggttccaggc ctcaggtctc
     6001 agatgaaaaa cccttggagt agaagagtgc catccctccc ggggttttga ctcgaaacac
     6061 gcaacccgga acccgcaact gtcttttacc tgaaacctga gacttggaac ctgggactgt
     6121 ctttatctct tgggtggttt cgtggtctcc agttcggcta tctcttgctc gatgtcgcgt
     6181 atggtgagcc gcatccgcgt gatggagccg atgcggtcac ggatgaaggg gtcatcggag
     6241 gaaacgctct tgttgcaggt aaactggtcg tacacgtact cacctatctc agcgttcacc
     6301 ttctgaatgg aacgctgcag ctttgcgacg tcgaggtact tccgcttccc gatcatcgcc
     6361 tgcttctcca cactgaacgc gatatcctgc gccgtctcct tcagcgtctt caacccttcc
     6421 tcggtcctct tccgtatatc atcaataagc gccaccacaa cctccgatcc aacatggatt
     6481 tttcaatatt ctgcaccttg aggcgccaaa ggtcaaacat aaagaccgct tgagccgctt
     6541 gagccgcttg agccgcttga acgggaaaag caatagacaa tgaagtgacc cctgatcgtc
     6601 atcccggtgt cgggccgagc tgtcttgttc tttcttctta acgttcaagc tgttcaagcg
     6661 gttgcaacgg tcttttccgg ttcaagccgc ctctagtaga gaaacatcgg tttccccagc
     6721 gtgtcgcgga cctttggacg gtacgtgtcc acg
//
LOCUS       DAOPOA010000028         6701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_12569_length_6701_cov_3.947186, whole genome shotgun sequence.
ACCESSION   DAOPOA010000028 DAOPOA010000000
VERSION     DAOPOA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12569_length_6701_cov_3.947186"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..971)
                     /locus_tag="PKJ17_01135"
     CDS             complement(156..971)
                     /locus_tag="PKJ17_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOD74598.1"
                     /translation="MNDISEKRFQNKLLERAFSILGSFSLDRKEQTLSELSRAVGLAK
                     STCHRLVSTLIAFGYLTYDESSKYYSLGPKLFELGSVVYDSISLRKIASQHLNDLLSK
                     VGNKAVALAIFEDDEIVYLDRKEDKRNPIKFSTTEVGRRRPPHFGMFGLLFMAYLGDD
                     EVERLLGKYPLVATTAKSARDSAEFKQRLKRVREVGYSFDDEGAFEDLSGVAAAIRDG
                     SNAVVGAVGCSFISASVNDETRQAVISAVCEAAARISGSLSNTVLPRAIWGRR"
     gene            1336..2274
                     /locus_tag="PKJ17_01140"
     CDS             1336..2274
                     /locus_tag="PKJ17_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD74599.1"
                     /translation="MNIRILSTSPLIGPHVKDLGRDFPESRIAPYRSAAWTEALPGAE
                     ALVVLLSEPLTEMDLEPCVNLKVIGTYSVGTNHLPRNYCDARGIRIVNTPGVLTDATA
                     DLALTLLLALTRRVREGEALVRSGEWKGWAPDLLLGSGLAGKTCGILGSGPIGRAFAR
                     RVRAMGMEVVFWDREDTGEPVDLGAETIRRLPLDDLLRGSSVLSLHCPLTDRTRGLLN
                     RENLALLPEGAFIINTARGGIVDEKAAIEFLHCGKIGGVGLDVYENEPDIDRQWRGAP
                     RTVLLPHLGSATRETREEMSRLLCDGIRNVLTSSGT"
     gene            2271..2978
                     /locus_tag="PKJ17_01145"
     CDS             2271..2978
                     /locus_tag="PKJ17_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD74600.1"
                     /translation="MMVDPGAIRLVLMPGLDGTGMFFGPLIQALPPDVPRTVITYPDD
                     KDFSLKDHADLVAARLTGEDTVLVAESFSGLVALMLLHARPASVKGVVFSAAFAEPLH
                     PLLIRAAASIPGAASLARRLPAGLLNVLFFHSHADKALKELLRRSVQLAGPAGLRRRA
                     RLIADGYPFPDDRFPLPCLYLQAAQDRVVPSRAADWFRTRFASFELVTLDAPHCLLQT
                     RPAESAEAVMAFARGLA"
     gene            3145..3723
                     /locus_tag="PKJ17_01150"
     CDS             3145..3723
                     /locus_tag="PKJ17_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD74601.1"
                     /translation="MEEARKPHGAGRSAFDLIDTDRVFEALNVRPSTVILDLGAGAGN
                     YTVRLAETTGPGGRVFALDAWEEGLARVRERASLERLGNIETLLADANESIPLEDGTI
                     DLCLVATVLHDLLRDATGETVLSEILRVLKRGGKLAVLEFKKIEGPPGPPLRIRLSEK
                     DVEGLLAPFGFHTDSVSDVGEYHYLLIASLPG"
     gene            complement(3845..5554)
                     /locus_tag="PKJ17_01155"
     CDS             complement(3845..5554)
                     /locus_tag="PKJ17_01155"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOD74602.1"
                     /translation="MAKKPANLLYDVDDRPPAGVCVVLAIQHIFFLTGGFIVVAIVMG
                     QMGCSPELIRNVVSITMIAGGMATILQALNRGPVGSGYLCTEGTDPSFLSISILAGSV
                     GGLPLIFGMTIVSGVIECLLSRVMHRLRVIFPPDVAGVVLTMVGLNIVPIMILDFMGV
                     KDRVSPVETANVLVASITLAIMVGMSVWGKGKLRLYSVIVGIAGGYAASILLGVLTVA
                     QMREVIDAPVVSVPDFSHVSYSFDPALIIPMVIVTLASTLKSVASLTMCQKVNDTAWV
                     RPDLVNIGKGTLADGLASIIGGGLGALGKSLYAASVGLTVATGATSRIIAWYIGFIFI
                     ALAFLPKLAAVFSIMPKPVMGGAMVYMVAFMVISGIQMMTSRMIDNRKTFVIAVSLMF
                     GMSVDIFPDLYRHAPAWLEPFLSSSLTVTTVLAIVLNLIMRIGISRRATLKVTSGEHS
                     SDIVFRFMEDLGASWGARKEVVYRAVAAMNEFAEAIVHCGMDGRQVLMTAVFDELSLD
                     IRITYEGPPVEFPEERPDMAAIIDDPEALARMSGFLVRHFTDRIRMSREGERTRVDLH
                     FDH"
     gene            6053..6244
                     /locus_tag="PKJ17_01160"
     CDS             6053..6244
                     /locus_tag="PKJ17_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD74603.1"
                     /translation="MNVLRAEHRITQEGLAKGVGVTRATIVAIEKGNYNPSLGLAFRI
                     ARYFKTDINDIFSVEEEKR"
     gene            6241..>6701
                     /locus_tag="PKJ17_01165"
     CDS             6241..>6701
                     /locus_tag="PKJ17_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74604.1"
                     /translation="MKRLDQVILKVFLCGLPVLAGIALFNYVFDAGLAAGHLKTAYNA
                     SGFLYAAWMLLSIYLSIRLVLSGAFRDRVLARLTFMRERDEREALLTGKATKTTLMTS
                     LAILIFLFCLSCFQVSFFKLPPDKALDGKTRGFSLGLKFSLFKDFEAGQSD"
BASE COUNT         1333 a         2070 c         1970 g         1328 t
ORIGIN      
        1 ctccccttca gcgaccgaga aggacgcgtt gttcaccgcc cagagggccc cgaacgattt
       61 tgatagatac tccaccttca acagatccat acgcttctac ctatcctcct gcggctcccg
      121 tccctcactc ttgcgaccgt ccgaatccga actcatcatc tcctgcccca gatcgctctc
      181 ggcagaaccg tgttgctgag gctccccgat atccttgccg ccgcttcaca tacggccgag
      241 atcaccgcct gtcgtgtttc atcgttcacc gatgccgaga taaaactgca tccaacggct
      301 cccaccaccg cattgcttcc atccctgatc gctgcagcga caccggagag gtcctcgaat
      361 gctccctcat cgtcgaatga atagcccacc tcccgcactc tcttcagcct ttgcttgaat
      421 tccgcggaat ctctggctga cttggcggtc gtcgcgacga gcgggtattt ccccagcagc
      481 cgctccacct catcatcacc aaggtacgcc atgaacagca ggccaaacat gccgaagtga
      541 ggaggcctcc gcctgcccac ttcggtggtg ctgaacttga tcgggttgcg tttgtcttcc
      601 tttctgtcaa ggtagacgat ctcatcgtcc tcgaagatcg caagggcgac cgctttgttc
      661 cccaccttgg acagcaggtc attcaggtgc tgcgacgcga tcttccggag cgatatcgag
      721 tcgtagacca cggaacccag ttcgaacaac ttcggtccca gagaatagta cttcgatgac
      781 tcatcgtagg tcagataccc aaaggcgatc agcgtcgaca ccagcctgtg gcacgtcgat
      841 tttgccaggc ccactgcccg tgacaattcc gataaagtct gttcttttct atcgagagag
      901 aaactgccca gaatgctgaa cgccctttcc aggagcttgt tttggaacct tttttccgag
      961 atatcgttca tatctcctcc gttggtgttc cgtccgctgg aacgctgttc catgagataa
     1021 tactattccc attggtccat ctttgtcaag gaaaaaccgc ccgttccagg aatgggcttg
     1081 tttttcgcag gatcgggctg gtaaccgctt gttaatgttc agggatccta ttacggcgca
     1141 gctcgacctg gcccgctgct cccgggggcg atcgacctcg acgacccgcc aggcgaggcg
     1201 gcgggacagt gcgggtatcc cttcgttttc tcctccctgc tgaaatacga aacacgagat
     1261 tgtcttgact tcgaaaacgg gcattactac actttcttga acagaacggt attgagcgga
     1321 aaggcggtca ggcaaatgaa catccggatc ctgagcacat cacccctcat aggtccccat
     1381 gtcaaggacc tcggtcgcga ctttccggaa tcgcggattg ctccttaccg ctccgccgcc
     1441 tggacggagg cgctccccgg ggcagaggcg cttgtcgtcc tcttgtcgga acccctgacg
     1501 gagatggacc tggaaccctg cgtgaacctc aaggtcatcg gcacctattc cgtgggcacg
     1561 aaccaccttc cccggaacta ttgtgacgca cggggcatcc gcatcgtgaa cacaccgggg
     1621 gtgttgacgg acgccacggc cgatctcgcc ctcacgctcc tcctggcgct cacccgccgg
     1681 gtgcgcgaag gcgaggcgct ggtgcggtcc ggggagtgga aaggctgggc tcccgatctt
     1741 ctcctgggat cgggcctcgc cgggaagacc tgcgggatcc tgggctccgg ccccatcggc
     1801 cgggccttcg ccaggcgcgt ccgggccatg ggcatggagg tcgtcttctg ggatcgcgaa
     1861 gacaccggcg aacctgtcga cctcggggca gagacaatcc ggcgcctgcc gctcgacgac
     1921 ctgctgcgcg gaagcagcgt tctgtccctg cattgtccgc tcacggaccg gacacgcggg
     1981 cttctcaacc gcgaaaacct ggcattgctc cccgaagggg ccttcatcat caacacggcg
     2041 cggggcggga tcgtggatga gaaggcggcg atcgaatttc tccattgtgg gaagatcggc
     2101 ggtgtgggac tcgacgttta cgagaacgag ccggacatcg atcggcagtg gcgcggggct
     2161 ccgcgaacgg ttctgcttcc ccaccttggg tccgcgacga gagaaacacg ggaggagatg
     2221 tccagacttc tctgtgacgg gatcaggaac gtcctcacaa gctcagggac atgatggtcg
     2281 atcccggcgc cataaggctt gtcctcatgc ccggcctcga tggcacggga atgtttttcg
     2341 gaccgttgat ccaggcacta ccgccggatg tcccgcggac cgtcataacc tatcccgatg
     2401 ataaggattt ttccctcaag gaccacgccg accttgtggc cgcccgactg accggtgagg
     2461 acaccgtcct cgtcgcggaa tccttttccg gtctcgtcgc gctgatgctt ctgcatgcga
     2521 ggcccgcgag cgtcaaaggc gtcgtcttca gcgccgcctt cgcggaaccc cttcatcccc
     2581 tcctgatccg cgccgccgcc tcgatcccgg gggcagcctc actggcgagg agattgccgg
     2641 caggtctcct caacgtcctc ttctttcact cccatgccga taaggccctg aaagaattgc
     2701 tgcgccgatc agtgcagcta gcaggacccg ccggtctcag gcggcgggcg aggctcatcg
     2761 ccgacggata cccgttcccc gatgaccgct tccccctgcc ctgcctctat cttcaggcgg
     2821 cacaggaccg ggtggtcccc tcccgggcgg ccgactggtt caggacccgc tttgcatcct
     2881 tcgagcttgt gacgctcgat gcgccgcatt gcctgctgca gacaagaccg gcggaatccg
     2941 cggaggcggt catggcgttc gcacgagggt tagcataagg aaagtcctct ccgcacactt
     3001 tccggagccg gacttccacc gcactgaggc agcgtcccct gcccccggga ttgacagcgg
     3061 aacccttgat gccgcacatt aaaggagcaa gacgcttctc cttaagaccg gggcgacata
     3121 gagcgaaaag ggggtttcaa gatcatggag gaggcgcgaa aaccacacgg tgcgggccgc
     3181 agcgcattcg atcttatcga cacggaccgt gtcttcgagg cgcttaacgt gcgcccgtcg
     3241 acggtcatcc ttgacctcgg agccggtgcc ggcaactaca cggtccgcct cgcggagact
     3301 accgggcccg gcgggcgcgt ctttgccctg gacgcctggg aggaagggct cgcacgggtg
     3361 agggagcgcg cgtcactgga acgcctcggc aacatcgaaa cgctcctcgc cgacgcgaac
     3421 gagtccatcc ccctcgaaga cgggaccatc gacctctgcc tcgtggcaac ggtccttcac
     3481 gacctcttgc gcgacgcgac gggagagacc gttctcagcg aaatactcag ggtcctgaaa
     3541 cggggtggga aacttgccgt gctggagttc aagaagatcg agggcccgcc aggcccgcca
     3601 ctcaggatac gcctttcgga aaaggatgtt gaaggccttc ttgcgccctt cggctttcac
     3661 accgacagcg tatccgacgt gggagagtat cattatctcc tcatcgcttc gctgcccggc
     3721 tgaagcaccg ggctggtccc gaacgtctgc cggtcggcct ctcaagaacc tgcgggggaa
     3781 cattccgggt gcgccccaag ccgggtctcc agagtgaggc attcctttcc gaagaaaacc
     3841 ttcctcaatg gtcgaagtgg aggtccaccc tcgtccgttc gccttcccgg gacatcctga
     3901 tccggtccgt gaagtggcgg acgagaaatc cggacatgcg ggcaagcgcc tccgggtcgt
     3961 cgatgatggc cgccatgtcg gggcgttcct cggggaattc caccggcggg ccctcgtagg
     4021 tgatgcggat atccaaactc agctcgtcga agacggcggt catgaggacc tgcctgccgt
     4081 ccattccgca gtggacgatg gcctccgcga actcgttcat ggctgccacg gccctgtaga
     4141 caacctcctt gcgggcaccc caggaggcgc ccaggtcctc catgaaccgg aacacgatat
     4201 ccgaggaatg ttccccggag gttaccttca gggtagcgcg gcgggagatc ccgatgcgca
     4261 tgatcaggtt gagaacgatc gcgagcaccg tggtgacggt gagggaggaa ctgaggaagg
     4321 gctccagcca cgcaggggcg tggcggtaca ggtcggggaa tatgtccacg ctcatgccga
     4381 acatgaggga gacagcgatg acaaaggtct tgcggttgtc gatcatccgg gaggtcatca
     4441 tctgaatgcc ggagatcacc atgaacgcga ccatatagac catggccccg cccatgactg
     4501 gctttggcat gatactgaag accgccgcca gtttcggcaa aaaagccagc gcgatgaaga
     4561 tgaacccgat ataccaggcg atgatcctgc tcgtcgcgcc ggtggccacc gtgagaccga
     4621 cgctggcggc gtagagggac ttccccaaag cccccaaccc accgccgatg atggaggcaa
     4681 gcccgtcggc cagggtgccc tttccgatat tgacgaggtc cgggcggacc caggccgtgt
     4741 cgttgacctt ctggcacatg gtgagagagg cgacggattt cagggtggag gccagggtga
     4801 cgatcaccat ggggatgata agggccgggt cgaaggaata gctgacgtgc gagaagtcag
     4861 gcacggaaac caccggcgca tcgatcacct cacgcatctg tgcgaccgtc agcaccccca
     4921 ggaggatgga ggccgcgtaa ccgcccgcga taccgacgat gacggaatag agacgcagct
     4981 tgcctttgcc ccacacgctc atacccacca tgatggcaag ggtgatcgac gcgacgagaa
     5041 cgttcgccgt ctcgacgggg ctcaccctgt ccttgacgcc catgaagtcg aggatcatga
     5101 tggggacgat gttaagaccc accatggtga gcaccacccc ggcaacgtca ggggggaaga
     5161 tgacgcgaag acggtgcatg acccgcgaca gcaggcattc gatgacgccg gagacgatgg
     5221 tcatgccgaa gataagcggc aatccgccca cgctgcctgc caggatggag atggagagga
     5281 agctcgggtc cgtaccctcg gtgcagaggt agccgctgcc cacggggccg cggttcaggg
     5341 cctggaggat cgttgccatg cctcccgcga tcatcgtgat ggacacgacg ttgcggatga
     5401 gctcggggct gcagcccatc tggcccatga cgatggccac cacgatgaaa ccgcccgtca
     5461 ggaagaagat gtgttggatc gccagcacca cgcacacgcc ggccgggggc cggtcatcga
     5521 catcatagag aagattggcg ggtttctttg ccataagctc atcacctctt ttttcttgga
     5581 tcagtcggca ggacctgctt tctgtggatt ctctaacacc tgcggtgtcc cccggtcggg
     5641 ccggcacagt cgccccgggt cagacccgac gaacgaaggc ccctttgagg tcaaagagta
     5701 ccggtctgct tcacgttcgc gcgccggcag ttcgccggca tgctacccac actttgcgtt
     5761 aaacacctgc cctgttgcct gtcggaatac ttcgagatcg cgaaccacct gaacaacgtc
     5821 cgccggaccc gatatcgtcg ctatagtctg tcaggccggg acatcgggca ccacaatgat
     5881 gggaccaacc gaactggaaa agggaccgga aggctccaaa ccccttttcg cgcacttgtc
     5941 tgtataatta acttgacatg gtgtaatatc aactatacta taagtaatat taactatact
     6001 tcaggtggga gagatgggaa gatcatgcga gccgttaagg attgtcaacc ggttgaacgt
     6061 attgcgtgcg gagcaccgta tcactcagga ggggctggcg aaaggggtcg gggtgacgcg
     6121 ggccaccatc gtcgcgatcg agaaagggaa ctacaaccct tccctggggc ttgcttttcg
     6181 gattgcccgg tatttcaaga cggatatcaa tgacattttt tcagtcgagg aggaaaagag
     6241 atgaaaaggc tggatcaggt tatactgaag gtgtttctct gcgggcttcc tgttctggcc
     6301 ggaatcgccc tgttcaacta cgtgttcgat gcagggcttg ctgccggcca cctcaagacg
     6361 gcgtataatg ccagcgggtt tctctatgcc gcgtggatgc tcctttccat ctacctgagc
     6421 atccggcttg tgctttcggg ggcgttccgg gacagggtgc tggccaggtt gaccttcatg
     6481 agggagcgcg acgaacgcga agccctcctg acggggaagg cgacgaagac cacgctcatg
     6541 acctcgctgg ccatcctgat ctttctgttc tgtctttcct gctttcaggt atctttcttc
     6601 aaattgccgc ccgacaaagc gctggatgga aagacccgcg gcttttccct gggtcttaaa
     6661 ttctcactct tcaaagattt cgaggctggt cagagcgata g
//
LOCUS       DAOPOA010000029         6598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_12796_length_6598_cov_3.895461, whole genome shotgun sequence.
ACCESSION   DAOPOA010000029 DAOPOA010000000
VERSION     DAOPOA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12796_length_6598_cov_3.895461"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..431
                     /gene="rplP"
                     /locus_tag="PKJ17_01170"
     CDS             15..431
                     /gene="rplP"
                     /locus_tag="PKJ17_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOD74605.1"
                     /translation="MLAPKRVRYRKVQKGTLKGVAQRGNVVSFGDFGLQATEGGWITS
                     RQIEAARIALTRYVKRTGKVWIRVFPDKPITKKPAETRMGKGKGPNEGWVAVVKPGRV
                     LYEITGVPEDKAREALRIASFKLPIGTKFITRSEEQ"
     gene            428..613
                     /gene="rpmC"
                     /locus_tag="PKJ17_01175"
     CDS             428..613
                     /gene="rpmC"
                     /locus_tag="PKJ17_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOD74606.1"
                     /translation="MKARELKELTKEELAKKKKDLKEELFNLRFQHSTGQLENTARMN
                     TLKKDVARIETVLSEKE"
     gene            632..940
                     /gene="rpsQ"
                     /locus_tag="PKJ17_01180"
     CDS             632..940
                     /gene="rpsQ"
                     /locus_tag="PKJ17_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOD74607.1"
                     /translation="MEAKREISKRKMVGRVIKDKMDKTVVVEVEKFLKHPKYHKFIKR
                     KLRYKAHDENNSSKTGDRVLIVETRPLSKDKRWLVKEVVRHEELFLVKNEVAHDSGEI
                     "
     gene            921..1289
                     /gene="rplN"
                     /locus_tag="PKJ17_01185"
     CDS             921..1289
                     /gene="rplN"
                     /locus_tag="PKJ17_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOD74608.1"
                     /translation="MIQERSKLEVADNSGAKKLGCIKVLGGSRKRYGTVGDIIVASVK
                     EVIPNSKVKKGEVVKAVIVRTKKEIRRNDGSYVKFDDNSAVIINQYNEPVGTRIFGPV
                     ARELRAKRFMKIVSLAPEVV"
     gene            1298..1621
                     /gene="rplX"
                     /locus_tag="PKJ17_01190"
     CDS             1298..1621
                     /gene="rplX"
                     /locus_tag="PKJ17_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOD74609.1"
                     /translation="MEKAYHVKKNDLVMVTTGKDKGKTGKVLRINYKKDRLIVEKVNM
                     VKKHVRPSQKAKGGIMEMESSIHVSNVMIYCEKCAKPVRTGTKVLEDGKKVRFCKKCD
                     EVIDK"
     gene            1636..2175
                     /gene="rplE"
                     /locus_tag="PKJ17_01195"
     CDS             1636..2175
                     /gene="rplE"
                     /locus_tag="PKJ17_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOD74610.1"
                     /translation="MNTYREFYEKEVKSALMRRFQYKNIMEVPKIVKITVNIGLGEAL
                     QNIKALDAASGDVKLITGQKPVITKAKKSIASFKLREGMSVGCMVTLRRERMYEFYRK
                     LVTIVLPRVRDFKGVSPKSFDGRGNYTLGLKEQIIFPEIDYDKIDKVRGMNITITTTA
                     KTDEEGHELLKLMGMPFRS"
     gene            2187..2372
                     /locus_tag="PKJ17_01200"
     CDS             2187..2372
                     /locus_tag="PKJ17_01200"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOD74611.1"
                     /translation="MARKAMIEKTKRAPKFKVRVRNRCAVCGRPRAFIRKFQMCRVCF
                     RSYSLRGEIPGVLKSSW"
     gene            2388..2783
                     /gene="rpsH"
                     /locus_tag="PKJ17_01205"
     CDS             2388..2783
                     /gene="rpsH"
                     /locus_tag="PKJ17_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOD74612.1"
                     /translation="MVDPIADMMTRIRNAITARHETVDIPYSNMKFSISKILKEEGYV
                     RNYKTFVDEGRKKFLKVYISYDENSKSVITGLKTISKPGRRVYAKVADMPRFRNRTGI
                     VVISTSKGLMTDKNAIKNKVGGEPLLVVW"
     gene            2793..3329
                     /gene="rplF"
                     /locus_tag="PKJ17_01210"
     CDS             2793..3329
                     /gene="rplF"
                     /locus_tag="PKJ17_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOD74613.1"
                     /translation="MSRIGKKPIILPQGTQLELKDGEVLVTGPKGSLQRPFLKGLTLG
                     IDGSTVTVSRTSEEKTIRGYHGLMRTLIANMVEGVSKGFEKKLEIIGIGYRAEFQSGS
                     FTFYLGYSHPIVFALPEGITGGLEKQTQVSVQGIDKELVGQVAAKIRALRKPDAYKNK
                     GIKYADEVLRKKAGKSGK"
     gene            3341..3706
                     /gene="rplR"
                     /locus_tag="PKJ17_01215"
     CDS             3341..3706
                     /gene="rplR"
                     /locus_tag="PKJ17_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOD74614.1"
                     /translation="MARKDKVEARQRRKRRIRKKIYGTEQKPRLTVFKSIKQIYAQLV
                     DDTQHKVLTGISTLNKEVQAGLKSGGNSDAAKKVGEAIGKKALGLGITEVVFDRNGFK
                     YHGRIKALADGAREAGLKF"
     gene            3719..4225
                     /gene="rpsE"
                     /locus_tag="PKJ17_01220"
     CDS             3719..4225
                     /gene="rpsE"
                     /locus_tag="PKJ17_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOD74615.1"
                     /translation="MVERKRIDAGDLELQDRLVYINRVAKVVKGGRRFSFSAIVVVGD
                     GNGKVGFGLGKANEVPDAIRKAVERAKKSLIEVPVEKGTIPHEIMAKFGTSQVYMKPA
                     REGTGVIAGRAVRAVVEVAGITNILTKCYGSRNYHNVVKATIRGLSQLKSPEVALKQR
                     GKLKVEEG"
     gene            4227..4412
                     /gene="rpmD"
                     /locus_tag="PKJ17_01225"
     CDS             4227..4412
                     /gene="rpmD"
                     /locus_tag="PKJ17_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOD74616.1"
                     /translation="MSHLKIKWTKSFIGCPEDQRATVRSLGFKRLYQEKTVKNSPEIR
                     GMVKKVMHLVTVLEDAR"
     gene            4409..4852
                     /gene="rplO"
                     /locus_tag="PKJ17_01230"
     CDS             4409..4852
                     /gene="rplO"
                     /locus_tag="PKJ17_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOD74617.1"
                     /translation="MKLSDLKPVPGSKKSRKRVGRGTGSGLGTTAGYGNKGQRSTSGG
                     TKGKSFEGGQLPLTRRIPKRGFKNPFRKEYSIINIGDLEVFKGMEKVGVEDILKSGFI
                     KKVKDGIKLLAVGDIDFPIKISVHKASQKAIEKIQAKGGDVEVLV"
     gene            4852..6159
                     /gene="secY"
                     /locus_tag="PKJ17_01235"
     CDS             4852..6159
                     /gene="secY"
                     /locus_tag="PKJ17_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004956156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOD74618.1"
                     /translation="MGGFQNIGKIPELKRRIIWTLGLLAVYRVGIHIPTPGIDGNVLA
                     GIFEQAKGTLLGFFDMFAGGGLERLSIFALGIMPYISASIILQLLTVVIPTLEKLSKE
                     GEAGRRKITQYTRYGTVVISIIQGLFIAIGLEQMRGAGGEPVVFNPGWSFRLMTMITL
                     TGGTSFIMWLGEQITEKGIGNGISLIIFAGIVARMPNAVAGTWSLVNTGELNWFVVLV
                     LMAMMLAVVAFIIFMETSQRRIPVQYAKRVVGRKMMGGQSTHLPLKVNTAGVIPPIFA
                     SSIIMFPATISNFITHPYAKKVAELFTPGSALHEIFYVGFIIFFCYFYTAIVFNPDNV
                     ADNMKKYGGYIPGIRPGKRTSEYIDRVLTRVTFIGAIYISFVCVLPTLLVKKFNVPFY
                     FGGTALLIVVGVALDTIQQIESHLILRHYDGLVKKSQRIKGRR"
     gene            6242..>6598
                     /locus_tag="PKJ17_01240"
     CDS             6242..>6598
                     /locus_tag="PKJ17_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOD74619.1"
                     /translation="MRVASGKAMEMLLYLKDFVKAGVKTMDLENACEEKIREVGNMKA
                     AFKGYNGFPYCLCVSVNDEVVHGMPSERQLKENDIVSIDFGLLYEGYYGDVAMTYAVG
                     GISKATRKLLEVTEKAL"
BASE COUNT         1780 a         1591 c         2025 g         1202 t
ORIGIN      
        1 caggaggctc ggtaatgctt gcaccaaaaa gggtaagata cagaaaagtg cagaagggta
       61 ccctcaaggg cgtcgcgcag aggggcaacg ttgtcagctt cggtgacttc gggcttcagg
      121 ccacggaagg cggatggata acatcccgcc agatcgaagc ggcccgtatc gcccttacga
      181 gatatgtcaa aaggacaggc aaggtctgga tcagggtgtt ccccgacaag cccataacga
      241 agaaacccgc ggaaacaagg atgggtaagg gcaaggggcc gaatgaagga tgggtcgccg
      301 tggtgaagcc cggcagggtc ctctacgaga tcaccggcgt tcccgaggac aaggcgcgcg
      361 aggccttgag gatcgcttcc ttcaagcttc cgatcggtac caagttcatt acgagaagtg
      421 aggaacaatg aaagcgagag agctgaagga actgacgaaa gaagagctgg caaagaagaa
      481 gaaggacctc aaggaggaac tcttcaacct gagatttcag cattcgacgg ggcagcttga
      541 gaacacggca cgcatgaaca cgctgaagaa agacgtcgcc aggatagaaa ccgtcttgag
      601 cgagaaggaa taaaggctta aagaggttcc catggaagct aaacgcgaaa taagcaagcg
      661 aaagatggtc ggcagggtca ttaaggacaa gatggataaa acggttgtcg ttgaggtcga
      721 aaagttcctc aagcacccga agtatcacaa gttcatcaag aggaaactga gatataaagc
      781 acacgacgag aataattcca gcaagacggg cgaccgggta ttgatcgtgg agacacgacc
      841 tctgagcaag gacaagcgct ggctcgtaaa agaggttgtc agacacgagg aacttttctt
      901 ggtcaagaac gaggtggcac atgattcagg agagatctaa gctcgaagtt gccgacaatt
      961 ccggtgccaa gaaactggga tgcatcaagg tgctcggcgg atcacgaaaa cgctatggga
     1021 cagtgggaga catcatcgtt gcgtctgtga aagaggtgat ccccaactcc aaggtgaaga
     1081 agggcgaagt ggtcaaggcc gtgatcgtca ggacgaagaa ggagatacgc aggaacgacg
     1141 gttcctacgt gaagttcgac gacaattcgg cggtcatcat aaatcagtat aacgagcccg
     1201 tgggaacgcg catcttcggc cccgtggcga gagagcttcg ggccaagagg ttcatgaaga
     1261 tcgtgtccct ggcccccgag gtggtgtgag gttgatcatg gaaaaggcgt atcacgtaaa
     1321 gaagaatgac ctggtaatgg tgacgaccgg aaaagacaag ggaaagacgg gaaaggtcct
     1381 caggatcaac tacaagaagg accggctgat cgtcgaaaag gtcaacatgg tgaagaagca
     1441 cgtgaggccc agccagaaag cgaagggcgg catcatggag atggagagtt ccatccatgt
     1501 ttcgaacgtc atgatctatt gcgagaaatg cgcgaagccc gtccggacgg gaaccaaggt
     1561 cctggaggac ggcaagaagg tgcgcttctg caagaaatgc gacgaggtca tcgataagta
     1621 agcgggaggc ggtcgttgaa tacgtatagg gagttctacg aaaaggaagt gaaatcggcc
     1681 ctcatgaggc ggtttcagta taagaacatc atggaagtgc cgaagatcgt gaagatcacc
     1741 gtgaatatcg gtctcggcga ggccctgcag aacatcaagg ccctcgatgc cgcctcgggc
     1801 gatgtcaagc tgatcaccgg ccagaaaccg gtcatcacga aggcgaagaa atccatcgcc
     1861 tccttcaagc tccgcgaagg catgtccgtg ggatgcatgg tgacactgcg cagggagagg
     1921 atgtacgagt tctaccgcaa actggtgacc atcgtccttc ccagggtgag agacttcaag
     1981 ggggtctccc cgaagtcctt cgacggcagg ggcaactaca ccctgggatt gaaagagcag
     2041 atcatcttcc ccgagataga ctacgacaag atcgacaagg tcaggggaat gaacataacg
     2101 atcacgacaa cggcgaagac ggatgaagaa ggccatgagc ttttgaaact catgggtatg
     2161 cctttcagga gttgacggag gagtgcatgg caaggaaagc aatgatagaa aagacaaaga
     2221 gggcgccgaa gttcaaggtg cgagtgcgca acagatgtgc cgtctgcggc aggccgagag
     2281 cctttatcag gaaattccag atgtgcaggg tctgctttag gtcctattcc ctgaggggcg
     2341 agatccccgg cgttctaaaa tcaagctggt gaggtggtaa gatgggtatg gttgatccaa
     2401 tagcagacat gatgacgagg atccgcaacg ccatcacggc tcgtcatgaa acggtagaca
     2461 ttccttattc caacatgaag ttctccattt ccaagatcct caaggaagag ggctatgtga
     2521 ggaattacaa gacctttgtc gatgagggca gaaagaagtt cctcaaggtc tacatcagct
     2581 acgatgagaa ttcgaagagc gtcatcaccg gtcttaagac gatcagcaag cctggcaggc
     2641 gcgtctacgc caaggttgcc gacatgccca ggttccgcaa cagaacgggt atcgtcgtca
     2701 tctccacgtc gaaaggtctc atgacggaca agaacgcgat aaagaacaag gttgggggag
     2761 agcccctcct cgtggtgtgg tgaggtgcag gtatgtcgag gatagggaaa aagccaatta
     2821 tactcccgca gggaacacaa ctggagctca aggacggtga ggtccttgtg acggggccca
     2881 agggctccct ccagagaccc ttcctgaagg gattgacgct gggcatcgac ggtagtaccg
     2941 tgaccgtgtc ccgcacgagc gaagaaaaga cgatcagggg gtaccacggc ctgatgagga
     3001 cgctcatcgc caacatggtg gagggcgtat cgaagggatt cgagaagaag ctcgagatca
     3061 tcggcatcgg ctacagggcg gaattccagt ccggaagttt caccttttat ctcggttatt
     3121 cacatcccat cgtctttgcg ctccccgaag gcatcaccgg cgggcttgaa aagcagacgc
     3181 aggtgtccgt ccaggggatc gacaaggaac tggtgggaca ggttgccgca aagatacgcg
     3241 ctttgagaaa acccgatgct tacaagaaca aaggcatcaa gtacgccgac gaagtcctga
     3301 gaaagaaagc aggcaaaagc gggaaataag gagtagcggt atggccagaa aggataaagt
     3361 cgaagcccgg cagagacgga aacggagaat acgcaagaag atctacggca ccgagcagaa
     3421 accgagactc acggtgttca agagcatcaa gcagatatac gcgcagctcg tggatgacac
     3481 ccagcataag gtcctcaccg ggatctcgac ccttaacaag gaagtgcagg ccggactgaa
     3541 aagcggcggc aacagtgacg ccgcaaagaa ggtcggcgaa gctatcggaa agaaggccct
     3601 cgggctcggc atcacagagg tggttttcga ccgcaacggc ttcaaatacc atggaaggat
     3661 caaggcgctc gccgacgggg cgcgtgaagc cgggctaaaa ttttaacggg aggttgtgtt
     3721 ggttgaaaga aagcgaatag acgcaggcga tctcgagttg caggacaggc tcgtctacat
     3781 caaccgtgtc gcgaaggttg tcaagggtgg ccgcaggttt tctttcagcg ccatcgttgt
     3841 cgtcggcgac ggtaatggaa aggtcggctt cggattgggc aaggccaacg aagtgcctga
     3901 tgcaatacga aaagccgttg aaagggcaaa gaagagcctt atagaagtcc ccgtggaaaa
     3961 ggggacgatc ccccacgaga tcatggcgaa gttcgggacg agccaggtgt atatgaagcc
     4021 tgcacgggaa ggcacgggcg tcatcgccgg ccgcgccgtt cgggccgtcg tcgaggtggc
     4081 ggggatcacc aatatcctta cgaaatgtta tggttccagg aactaccata acgtggtgaa
     4141 ggcaacgatc aggggcctct cccagttgaa gtcgcccgag gtggcgctga aacagagggg
     4201 caaattgaaa gtcgaggagg ggtaggatga gccacctcaa gataaaatgg acgaagagct
     4261 tcatcgggtg cccggaagac cagagggcga cggtgagaag cctgggtttc aaaaggctct
     4321 accaggaaaa gacggtaaag aactccccgg agatacgggg catggtcaaa aaggtgatgc
     4381 accttgttac agtactggag gatgctcggt gaaactatca gatcttaaac cagtcccggg
     4441 atcaaaaaag agccgcaagc gtgtcggccg ggggaccgga tcggggctcg gaacgacggc
     4501 gggctacggc aacaagggcc agcgctcgac gagcggcgga acgaagggta agagcttcga
     4561 gggcggtcag ctgccgctga cgagaagaat ccccaagaga ggtttcaaga acccctttcg
     4621 caaggaatac tccatcatca acatcggcga cctcgaggtc ttcaagggca tggaaaaggt
     4681 cggcgttgag gatatcctga aatcgggttt catcaagaag gtgaaggacg gcatcaagct
     4741 tctcgccgtg ggagacatag atttccccat aaagatatcg gttcacaagg catcacagaa
     4801 ggccatcgaa aagatacagg ccaagggtgg tgacgtggag gtgctcgtct aatgggaggt
     4861 ttccaaaata ttggaaagat ccccgagctg aagcgcagga tcatatggac cctcggtctg
     4921 cttgcggtct accgtgtcgg catacacatc cccacgccgg gtattgacgg gaatgtcctg
     4981 gctggcatct ttgagcaggc caagggaacc ctgctcgggt ttttcgacat gttcgccggc
     5041 ggcggactgg agaggctctc catcttcgcc ctcgggatca tgccctacat ttcggcgtca
     5101 atcatacttc aactcctgac cgtcgtcata cccaccctcg agaagctctc gaaggaaggt
     5161 gaggcgggac ggaggaagat tacccagtac acgcggtatg ggaccgtcgt tatcagcatc
     5221 atccagggcc ttttcattgc catcggcctt gaacagatgc gcggcgccgg cggcgagccc
     5281 gtcgttttca accccgggtg gagcttcagg ctgatgacga tgatcacgct cacgggcggc
     5341 acgtccttca tcatgtggct cggagagcag atcaccgaga agggcatagg caacggcatc
     5401 tcgctgatca tctttgcggg catcgtggcc cgtatgccga acgccgttgc agggacatgg
     5461 agcctggtca acaccggtga gctcaactgg ttcgtggtac tcgtcctcat ggcgatgatg
     5521 cttgccgtcg ttgccttcat aatattcatg gagacatcgc agcgcaggat tcccgtgcaa
     5581 tacgcgaaac gggtcgtcgg gcggaagatg atgggcggtc agtcgaccca cctgccgctg
     5641 aaggtcaaca ccgcgggcgt tatcccgccg atcttcgcgt catccatcat catgttcccc
     5701 gcgacgatct cgaacttcat cacgcacccc tatgcgaaga aggtcgcgga gctcttcacg
     5761 ccggggtcgg ccctacacga gatcttctac gttggtttca taatcttctt ctgctacttc
     5821 tacaccgcca tcgttttcaa ccccgacaac gtggcggaca acatgaagaa gtacggagga
     5881 tacataccgg ggatcaggcc gggcaagcgg acctccgagt atatcgacag ggtccttacg
     5941 agggtgacat tcatcggggc gatatacatc tccttcgtgt gcgtgctgcc gacgctgctc
     6001 gtgaagaaat tcaacgtgcc cttctacttc ggtggcacgg cgcttctcat tgttgtcggt
     6061 gtcgccctgg ataccattca gcagatagag tcccatctca ttctgagaca ctatgacggg
     6121 ctggtgaaga agtcgcagag gatcaaggga cgacgataga aggaccaagg gggaatgccg
     6181 gcgggaaacg ccttgagcca aagggatgat ctttctgaaa acaaaagagg agattgaaaa
     6241 aatgcgggtc gccagcggga aggcgatgga aatgctcctc tacctcaaag atttcgtgaa
     6301 ggcgggcgtg aagacgatgg acctcgagaa tgcctgcgag gagaagatca gagaagtggg
     6361 caacatgaag gccgccttca aggggtacaa cggtttccca tactgcctgt gtgtgtccgt
     6421 gaacgacgag gtcgtgcacg gcatgccctc tgaacggcag ctgaaagaga atgatatcgt
     6481 gagcatcgat tttggccttc tctatgaagg gtattacgga gacgtggcaa tgacctatgc
     6541 cgtcgggggt atctcgaagg ccaccagaaa gctcctcgaa gtgacggaaa aagccctc
//
LOCUS       DAOPOA010000030         6533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_12944_length_6533_cov_3.716270, whole genome shotgun sequence.
ACCESSION   DAOPOA010000030 DAOPOA010000000
VERSION     DAOPOA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12944_length_6533_cov_3.716270"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PKJ17_01245"
     CDS             complement(<1..631)
                     /locus_tag="PKJ17_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOD74620.1"
                     /translation="MTERDVSISEFDRLCGWPDMCRSLFRDLITSIFEGFFITDGQGA
                     FLYASPSLATMLDYDPAQLLRMSIDDVDPERGFREAFAAMGAESYSVSTYETTLVRMG
                     GQPVDVEVTLARYNVPAEEQRCVGLVRHIGDRKRAETALRESEERFKLFSHASHEALV
                     LLNDAGRVLYVNPAALKITGRDEKQASGMEICEFLRPYQFSDEQRLQMAI"
     gene            821..2737
                     /gene="ftsH"
                     /locus_tag="PKJ17_01250"
     CDS             821..2737
                     /gene="ftsH"
                     /locus_tag="PKJ17_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOD74621.1"
                     /translation="MPKEKPRPKKDLKKRKDIILEIKGGNKDKEKKTKSFTFLYFIIA
                     IIGIFVIHSYLSFRSEIKTVPYSEFKGLVAQMKVSDLIIDAEKIQGTMTQQDGKKVKF
                     VTSRVEDPDLVKDLQKSNVQFSGSFENKILRVIIEWILPFAIIILIWNLLMRRMGGAP
                     SSVLNFGKSRGKIYGEDEIKITFEDVAGVDEAKEELQEIIEYLRYPQKFLDLGGKIPK
                     GILLVGPPGTGKTLLAKAVAGEAKVPFFSMSGSDFVEMFVGVGASRVRDLFSQAQQKA
                     PCIIFIDELDALGKARGMNPLSSHDEREQTLNQLLSEMDGFDTKTGVIIVGATNRPEI
                     LDPALLRPGRFDRHVLVDRPDIKGREEILKVHIREVKLGHQVDLSVIAARTPGFVGAD
                     LANLINEAALLAARKGKSSVSMEEFEEAIDRVVAGLEKKKKVMSKKEKEIVAYHETGH
                     ALMAESLEGADPVHKISIIPRGISALGYTMQLPTEDRYLMTKSELIDRMCVLLGGRIA
                     EEVIFGEISTGAQNDLARATDIARSMVKQYGMSEKLGHMTFEQERKPLFLDITPGSGA
                     KEYSEETAREIDNEVKAIIEHSYTKVKDTLTKKRDLLELVARTLLEKESIDGEELRNM
                     LKEHAEGDHDNESR"
     gene            2718..3611
                     /locus_tag="PKJ17_01255"
     CDS             2718..3611
                     /locus_tag="PKJ17_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74622.1"
                     /translation="MITNLDKKQIDLIKESLCVYEKARECRSLLRQEQLFFANIEDFV
                     DDRGKSCLFRLKEMCHDLFRNSSEASYKEKLFDMTVGYTFHGAMKLRENLYLLEYYKP
                     QCEIALEDLTDQERKIVNEISTLVRKARARLKEELKEVTILADELVKQLKDLILLYRG
                     NYLLPRFLYECEKTLTRIYGRRGFEDILITAYADGRSLLIFKTANSYLESEYFDTARK
                     LFKRIIRSDSSNTAALFLYLFASANHFYFKNMFTRALALAKKAESMNPDAGIREKYRP
                     LLTRLIADLSKEIKKKSDERR"
     gene            3829..5286
                     /gene="tilS"
                     /locus_tag="PKJ17_01260"
     CDS             3829..5286
                     /gene="tilS"
                     /locus_tag="PKJ17_01260"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOD74623.1"
                     /translation="MTGNGLPGTPGTPPGARPADLVRKISRLIRMEGMIGERDRVLVG
                     FSGGVDSATLLFVLREIRGKIPFDLAVAHVNHLLRREESERDEAFVRETAGSYGIPCF
                     VERADVKAYARSTGLSIQHAGRDIRYRFFAETADREGYTKIALAHNLDDQVETFLLRL
                     AKGTGIHGLSSIPPSREKIVRPFLTTYRSEIESYAAAHSIAFVTDSSNDKTVYERNYI
                     RHKVIPLLCALNPAFREKVVSLLGDLTEINRFFDEKKRAFTKKNVRRTEGDIHLPVGP
                     LLKLDEETFFRVVSDIVAGMAPVVPLREHIGLVRKVLSSRRPNLRLDLPEHLRVTKTY
                     GNLIFTTKRPAAQVTETFPLGEGRTRIAALGMNVTLTVLKRPPSSYPKDNFTAYFDLD
                     KLDPRDGLAVRTFRRGDRFQPLGMDKPVKIKDFFISRKIPLESRRLVPLLVSGDVIVW
                     VAGHRIDHRYRIDENTRRVLKVTVKKSCQPSSNCD"
     gene            5463..>6533
                     /locus_tag="PKJ17_01265"
     CDS             5463..>6533
                     /locus_tag="PKJ17_01265"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOD74624.1"
                     /translation="MLSARAASLKPSPTLAISAKEKALKAQGVDIAGFGAGEPDFDTP
                     VHIKEAGIKAINDNFTRYTPVTGIDPLKDAIIDKVARDNGLAYKREEVIVSCGGKHGL
                     YNLFQALFQEGDEVIVPTPYWVSYPPMIELADAKPVILETREEDDYQITPDLLAKYLT
                     PRTKAIILNYPSNPVGSVFYRENLEAIGKLAVEKGFYLISDEIYEKLVYDDYKHVSIA
                     SMDPAFKERTIICHGVSKTYAMTGWRIGYSAGPAAIIKAMGNIQSQSTSNPTSVSQMA
                     ALAALSGKQDFIGTMVAEFKKRKDFLVKSLRSIEGVTCYDPRGAFYVFPNFNVVMGKK
                     YKDRIVDSSTALTEILLDDFHTA"
BASE COUNT         1568 a         1966 c         1767 g         1232 t
ORIGIN      
        1 ctatcgccat ctgaagcctc tgttcgtcgg agaactgata aggcctgagg aactcgcaga
       61 tctccatgcc ggaggcctgc ttctcatcgc gtcccgtgat cttgagggcg gccgggttca
      121 cgtaaaggac cctgcccgcg tcattgagga gcacgagggc ctcatggctc gcgtgactga
      181 atagcttgaa cctctcctcg ctctcccgga gcgccgtttc ggccctcttg cggtcgccga
      241 tgtgccgcac caacccgacg cacctttgtt cctccgccgg aacgttgtac cgggcaaggg
      301 taacttccac atccacgggc tgcccgccca ttctcaccaa agtggtctca tacgtggaaa
      361 cgctgtaact ctccgctccc atggccgcga aagcctctct gaaccccctc tccggatcga
      421 cgtcatcgat ggacatccgg agaagctggg cgggatcata atccagcatc gtcgccaggg
      481 aaggactcgc atagagaaag gctccctggc cgtcagttat gaagaaaccc tcgaagatgg
      541 aggttatgag gtcccggaac aggctccggc acatatcggg ccaaccgcac aggcggtcaa
      601 actcgctgat ggatacatct ctttccgtca cctgccaccc tccttgcagc ggacaacgta
      661 atcgggcagc gggtctgcca taaggctatt atagcagctg acttcgtttt gtaaaccagc
      721 ggcatgcggc gcgccggcgc cgcaaccaca ggcccgtcag ccgccgcggg acactgagaa
      781 acatatttca aagctcatga ttttctgata tgctttacct atgccaaaag aaaagccccg
      841 gcctaaaaaa gacctgaaaa aacgcaaaga tataatcctc gagatcaagg gcgggaacaa
      901 ggacaaagag aagaagacga agagtttcac cttcctctac ttcatcatcg ccatcatcgg
      961 catattcgtg atccacagct acctgtcctt ccgttcggag atcaagacgg tcccctacag
     1021 cgagttcaaa ggtctcgtcg cccagatgaa ggtctcggac ctcattatcg acgccgaaaa
     1081 gatccagggg acgatgacgc agcaggacgg caagaaggtg aagttcgtga cgagcagggt
     1141 cgaggacccc gatctcgtca aggacctcca gaagagcaac gttcagttct ccggttcctt
     1201 cgagaacaag atcctgcggg tcatcatcga atggattctc cccttcgcca tcataatcct
     1261 catctggaac ctccttatga gaaggatggg cggagccccg tcgagcgtgc tcaatttcgg
     1321 gaagagccgc ggcaagatct acggtgagga tgagataaag ataaccttcg aggatgtcgc
     1381 cggtgtcgac gaggcaaaag aagagctcca ggagataatc gaataccttc gttatccgca
     1441 gaagttcctc gatctcgggg gaaagatccc caaaggcatc cttctcgtcg gtccgcccgg
     1501 cacgggcaag acccttctcg ccaaggcggt cgcgggcgag gcaaaggtgc cctttttcag
     1561 tatgagcgga tccgatttcg ttgagatgtt cgtcggagtc ggcgcgtcgc gggtgaggga
     1621 cctcttttcc caggcccagc agaaagcacc gtgcatcatc ttcatcgacg aactggacgc
     1681 ccttggcaag gcaaggggta tgaaccccct ttcatcccac gacgagcggg agcagaccct
     1741 gaaccagctt ctctccgaaa tggacggctt cgacacaaag acgggggtca tcatcgttgg
     1801 cgcgacgaac aggcctgaga tactcgaccc cgctctcttg aggcccggca ggttcgacag
     1861 gcatgtcctc gtcgacaggc ccgacatcaa gggccgtgaa gagatactga aggtgcacat
     1921 cagggaggtc aagctcggac accaggtcga cctctccgtc atcgccgcca ggacccccgg
     1981 ttttgtgggc gccgacctgg caaaccttat caacgaagct gcccttctcg ccgctcggaa
     2041 aggaaagtca tcggtctcga tggaagagtt tgaagaggcc atcgaccgcg tcgtggccgg
     2101 cctcgagaag aaaaagaagg tgatgagcaa gaaggaaaag gagatcgtcg cctatcacga
     2161 gacgggccat gccctcatgg cggagtccct ggagggcgct gaccccgtcc acaagatatc
     2221 catcatcccc cgcggcatat ccgccctggg atataccatg cagcttccca ccgaagaccg
     2281 ctacctcatg acaaagagcg agctcatcga ccgcatgtgc gtcctcctcg ggggaaggat
     2341 agccgaggag gtcatcttcg gcgagatctc gacaggcgcc cagaacgacc ttgcacgcgc
     2401 gaccgacatc gcgcgttcca tggtgaagca atacgggatg agcgagaagc tcggccacat
     2461 gaccttcgag caggaacgaa aacctctctt cctcgacatc acccccggct cgggcgcgaa
     2521 ggaatacagc gaggagacag caagggagat cgataacgaa gtgaaggcca tcatcgaaca
     2581 ctcctacaca aaggtcaagg acaccctcac gaagaagcgc gacctcctgg agcttgtcgc
     2641 caggaccctc ctcgaaaagg aatccatcga cggtgaagag ttgaggaata tgctgaagga
     2701 gcacgcggag ggggaccatg ataacgaatc tcgataagaa acagatagat ctcatcaagg
     2761 aatcgctgtg cgtctatgag aaggcgcggg aatgcaggtc cctgctccgc caggaacagc
     2821 ttttcttcgc caacatcgag gatttcgtgg acgacagggg caagagctgc cttttccggc
     2881 tcaaggagat gtgtcacgac ctcttccgca actcgtcgga ggcgagctac aaggaaaagc
     2941 ttttcgatat gaccgtcggt tacacctttc acggcgcgat gaagctccgc gagaacctct
     3001 acctcctgga gtattacaag ccgcagtgcg agatcgccct tgaggacctg actgatcagg
     3061 aaaggaagat cgtcaacgag ataagcacgc tggtgcgaaa agcgcgtgcg cgcctgaagg
     3121 aggaactcaa ggaggtcacc atcctcgcgg acgagctggt caaacagctc aaggacctca
     3181 tccttctcta ccggggaaac taccttctcc cccgcttcct gtatgaatgt gaaaagaccc
     3241 tgacaaggat atacggacgc aggggtttcg aagacatcct gatcacggcc tacgcggacg
     3301 gcaggtccct tctcatcttc aagacggcca acagctatct ggagagtgag tacttcgaca
     3361 cggccaggaa gctcttcaag cgcataatca gatcagacag ctccaacacg gccgccctct
     3421 tcctctacct cttcgcctca gccaaccatt tctacttcaa gaacatgttc acgagggccc
     3481 tcgcgctcgc gaagaaggct gaaagcatga accccgacgc cggcatccgc gagaaatacc
     3541 gcccgttact gacacggctc atagccgact tgtcaaagga gataaagaag aagagtgacg
     3601 agcggaggta accatgccta tatatgagta tacctgccgc acgtgcggca atgaattcga
     3661 agtgatagtc ttcggcgacg atgtgcccga atgccctgag tgcggggcca aagacccgca
     3721 gaagaacatg tcctcctttg gattctcctc agggtccaaa ttcaagtctt cgggcaagtc
     3781 gggcggctcc tgcaccggat gctcctcgcc tgactgttcg agttgttcgt gacagggaac
     3841 ggtctccccg gtactcccgg cacaccaccc ggggcgcggc cggcagacct cgtccggaag
     3901 atatcccggc tcatcaggat ggaaggtatg atcggcgaga gagaccgcgt ccttgtcggg
     3961 ttctccggtg gcgttgattc ggcgacgctt ctcttcgttc tcagggagat ccgggggaag
     4021 ataccttttg acctcgccgt tgcgcacgtg aatcacctcc tgaggcggga ggaatcggag
     4081 agagacgagg ccttcgtgag ggaaacggca gggagctacg gtattccctg tttcgtcgaa
     4141 agggcggatg tgaaggctta cgcccggtcc acgggacttt ccattcaaca cgccggcagg
     4201 gacatccgat accgtttctt tgccgaaacg gctgatcgcg agggttacac gaagatcgcc
     4261 ctggcccaca atcttgacga tcaggtcgag accttccttc ttcgcctcgc gaaagggacg
     4321 ggcatccacg gcctgtcttc gatacctccc tcccgcgaga agatcgtgag gccctttctc
     4381 acgacctacc gttccgagat agagtcctat gccgccgccc actccatcgc cttcgtcacc
     4441 gattcctcaa acgacaagac ggtgtacgag cgcaattaca ttcggcacaa ggtcatcccc
     4501 cttctttgtg cattgaaccc ggcattcagg gaaaaggtcg tctccctcct cggagacctg
     4561 acggagataa acagattctt cgacgaaaag aaacgtgcct tcacgaaaaa gaacgtccgg
     4621 aggacggaag gcgacatcca cctgcccgtc ggacctctcc tgaagctgga cgaagagacc
     4681 ttcttcaggg tcgtcagcga cattgtggcc ggtatggcgc ccgtcgttcc cttgagggaa
     4741 catatcgggc tcgtcaggaa ggtgctgtcc tctcgaagac cgaacctgcg tctcgatctc
     4801 cccgaacatc tcagggtaac gaagacttac ggcaacctca tattcacaac gaagaggccg
     4861 gcggcacagg tcacggaaac ctttcccctc ggcgagggga ggacccggat cgccgcgctt
     4921 gggatgaacg tcacactcac tgtattgaag aggcctcctt catcctaccc gaaggacaat
     4981 ttcacggcat acttcgacct cgacaagcta gatccccggg acggcctagc ggtccggacc
     5041 tttcgccggg gtgaccggtt ccagcccctc ggcatggaca agccggtcaa gataaaggat
     5101 ttcttcatat cccgcaagat acccctcgag tcccgcagac tggtccccct cctcgtctca
     5161 ggcgacgtca tcgtctgggt cgcgggccac aggatcgacc atcggtacag gatagacgag
     5221 aacacccgca gagtcctgaa ggtcactgtg aaaaaatcct gtcaaccatc ttcaaattgt
     5281 gattgacaga gccttccctc atggtgtaaa agttaccgct gtacccatac gggatcagca
     5341 gggagtgagg agcaaggagc agggagtgag gagtccttaa acaagaagat ccggtgaggc
     5401 ttcgcgattc cttccctttt tctgttcact ctgcacccaa aaaaagctaa aggagttccg
     5461 ttatgctatc cgcaagagcc gcctcactga aaccgtcacc cacactcgcc atctcggcaa
     5521 aggagaaggc cctaaaggcg cagggggtcg atatcgccgg cttcggcgcc ggcgagccgg
     5581 acttcgacac ccccgttcat atcaaggaag cgggcataaa ggccatcaac gataacttca
     5641 cccgctatac tcccgtcacc ggcatcgatc cccttaagga cgcgataata gacaaggtcg
     5701 cccgcgacaa cgggcttgca tacaagcggg aagaggtcat cgtttcctgc ggcggtaaac
     5761 acggcctcta caaccttttc caggcccttt tccaggaagg tgacgaggtt atcgttccca
     5821 ccccctactg ggtatcctac ccccccatga tcgaactcgc ggatgccaaa cccgtcatcc
     5881 tcgaaaccag ggaggaagac gactaccaga tcaccccgga cctcctcgcg aaatacctta
     5941 cgcccaggac gaaggcgatc atactgaact acccttcgaa ccccgtcggc tccgtcttct
     6001 atcgggagaa cctcgaggca ataggcaagc tcgcagtgga aaagggtttc tacctcattt
     6061 ccgacgagat atacgaaaaa ctcgtctatg acgactacaa acacgtcagc atcgcctcca
     6121 tggacccggc gttcaaggag cggacgatca tatgccacgg cgtctccaag acctacgcga
     6181 tgacgggctg gaggatcggc tacagcgcgg gccccgccgc catcataaag gccatgggga
     6241 acatccagag ccagagcacc tccaacccga cctccgtgtc gcagatggcg gccctcgcag
     6301 ccctttccgg caagcaggac ttcatcggca ccatggtcgc cgagttcaag aagagaaaag
     6361 actttctcgt taagagctta aggagcatag aaggcgtgac ctgctatgat ccccggggcg
     6421 ccttctacgt gttccccaac ttcaatgtcg tcatgggcaa gaagtataag gaccgcatcg
     6481 tcgattcctc aacggccctg acggagatcc tcctcgacga cttccacacc gcc
//
LOCUS       DAOPOA010000031         6490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_13054_length_6490_cov_4.678632, whole genome shotgun sequence.
ACCESSION   DAOPOA010000031 DAOPOA010000000
VERSION     DAOPOA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13054_length_6490_cov_4.678632"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1114
                     /locus_tag="PKJ17_01270"
     CDS             98..1114
                     /locus_tag="PKJ17_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD74625.1"
                     /translation="MGEVRAMVIPVFLPHSGCNERCVYCHQGYITDSGGADIKARIDG
                     ALRDRREACEVGLFGGNIFGMEPADLERLFSFFAPYKDVVKGFRLSTKPVPLRDDAID
                     ILKRNGVRMIELGIPTFNDAILAALNRAHTAKDLFRAFDRLLGEGFRLALQFMVGLPG
                     ETMVDIEETVENMVRLRPEYIRIYPLVILRNTPLYRLHETGGFTPIAFDEALERAALI
                     YRRALEEGIDVVNVGLTDNELVREMVAGGFYHPAFGSLVQSRIFRDALGSVLGEIEGP
                     KEVTVVLHRNDIPLLVGYRRENLKHFASLGIALRWEPSGAERGRFEVVSGGERVTGRM
                     IQRR"
     gene            complement(1180..2073)
                     /locus_tag="PKJ17_01275"
     CDS             complement(1180..2073)
                     /locus_tag="PKJ17_01275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD74626.1"
                     /translation="MKEQPLVSIIVPAYNYGTYVHRAIGSCLRQSSGNLEVIVIDDGS
                     TDATGEIVRKIADQRVVYHYQENQGVSAARNKGLRMAKGDFIAFLDADDYLTDDSVET
                     RVTVMLENPDIDFVTTTASSVDDAGRVSFRPYRGPRDIVSRELCQALLLKRIPYTTSA
                     VLVRGSHARKFEFATDLNNGEDLVFFSKVFFDRKGCFLSKPTAVTFSHPDSLRHNIAN
                     LRTQGAGLIEAIFDDPYFAGRLEHIRRPFAANRYVELFRRFYRSGDDKLAREYYRKAL
                     ALEPSRIFKIDHLFKFIRTWF"
     gene            complement(2080..3201)
                     /locus_tag="PKJ17_01280"
     CDS             complement(2080..3201)
                     /locus_tag="PKJ17_01280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD74627.1"
                     /translation="MKIGLAIYNFDPKKGGAERYTFDLARRLAGKGHEICVFCGRGVE
                     TEGVRLIRLRTNSFPRWRRNLSFALTHRQALDRIRPDIMLGYGNILDLDVYQSHGGVQ
                     SVWMEREIVSYGPGGAGMKAFLLRNSLNQAIQRWIEGYSVKKHRFTRIVAISDMVRDH
                     MSAHYGIDRNTFDVVYNGVDTDRFKPAPERPAGPTTILFCAGNFRLKGLSPLIAAMGR
                     AVEKGKDVRLTVMGRGKKERYRGLIDAAGIADRVTFTGERSAPEEVYREAHVLAHPTY
                     YDACSLTTMEGMASGLPIITTRWNGACAFVSPDEGFVIDEADNIEALSAAICALTDPV
                     RQEVMGRNARKKMESFTMERNADEMEAVLLGAFEEKGRR"
     gene            complement(3243..4376)
                     /locus_tag="PKJ17_01285"
     CDS             complement(3243..4376)
                     /locus_tag="PKJ17_01285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOD74628.1"
                     /translation="MKVLHLFSDWKWTGPAEPVLSLCENLGNEGVDVVLACRKSPADF
                     RERTVDREAGKRGLRCYEGLRLNRYFSARDWWHDLISFNPYVLKEKFDIVHTHLSHDY
                     YTAVTALSFSRARPLLVRTDHKRDGLPANIFMAWAMARTDGLVTYSERLAARDRESFA
                     YPGERLRVIPPGVKPYEGPLKDLRAELGIAPDERVIGVIGRLKPDRGYDVILSAFGML
                     RSRVDRVKLVIVGRSSQIETSVMKPLKELGIEKDVVIAGYRIDDYFSMIHTFDLYIMM
                     RAGSDGSGRALREVLSMGRPAIVSDRGMLPDLVEDGRTGYVTTWDAADLAGKMELLVS
                     DAARRRAFGENARNRADSEWSFRLQAKAMKQFYDFLLTSGRRA"
     gene            complement(4373..5482)
                     /locus_tag="PKJ17_01290"
     CDS             complement(4373..5482)
                     /locus_tag="PKJ17_01290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD74629.1"
                     /translation="MNVRIFDIEKDHAGIKAELVRTFEKVLSSGEYILGREVKSFEEA
                     FAACVGTRYAVGVASGTDAIKIGGLALGLTEGDKFVTTPNTYIATAMALSEGGLVPRL
                     CDIEADTYNMDPAALEELLGKEKDIKLCVPVHLYGQPCRMDEIRDICERHGVRVMEDA
                     CQAHGSRYKDRNAGTMSDVAAFSFYPTKNLGCYGDGGIVVTDREDVFRRMLMLRAHGQ
                     SDRHVHDIQGFVSRLDELQAALLSVKLPHLDGWNEGRRRNATLYDRGLEGLPVRRPVT
                     VPQAFHVYHLYVIAVEEREALRAYLSEKGVTTLVHYPTPIHLQKVYSYLGYGEGSFPK
                     AEKASRQIISLPVHPSLREEEIGFVCRTIGEFYGR"
     gene            complement(5524..>6490)
                     /locus_tag="PKJ17_01295"
     CDS             complement(5524..>6490)
                     /locus_tag="PKJ17_01295"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOD74630.1"
                     /translation="KDVLVTGGLGFIGSNLCIELVRLGARVTIIDNMLPRQGGNLFNI
                     RDIADRVTVNVSDVRNQLSMNHLVKGKDYIFHLAGQVNHVDSMKNPLQDLEINCTGTL
                     VLLEALRHNNRDARVIFAGTRGEYGSSVRLPVDEDHPTNPKGIYAVTNLTAEKMVLVY
                     DDVYKIRGVCLRITNTYGPRHQMAHDEYGVFNWFIRKALDDEEIPVFGDGRILRDFLY
                     VEDLVDSILKVAAAEEAYGKVFNVGTGVPVSFFELAEKIVAIAGTGRTRFTEFTQERK
                     EVEPGDYYADITRITGTTGWQPATSLDEGISRTIDFYRKHRMEYW"
BASE COUNT         1151 a         1913 c         1988 g         1438 t
ORIGIN      
        1 ttcagtatga cagccggcaa ggtgcgaggc ataccggttt cccggtcgtg cccgcgttca
       61 taacgatttg cttttttggc tcaaaagagt ggacaatatg ggcgaggtcc gcgcgatggt
      121 catacccgtg tttcttcccc attcaggctg caacgaacgt tgcgtttact gtcatcaggg
      181 ctacatcacc gacagcgggg gcgcggatat caaggcgagg atcgacggcg cgctcaggga
      241 caggcgcgag gcctgtgagg tgggcctctt cgggggcaac atcttcggga tggagcccgc
      301 ggacctcgaa aggctttttt ccttcttcgc cccttacaag gatgttgtaa agggcttccg
      361 cctctcgacg aaacccgttc cgctccggga cgacgcgatc gacatcctca agcgcaacgg
      421 cgtccgcatg atcgagctcg gcattcccac cttcaacgac gccattcttg ccgcccttaa
      481 cagggcccac acggctaagg acctctttcg cgcctttgac aggctcctcg gggagggttt
      541 caggcttgcc cttcagttca tggtgggctt gcccggcgag acgatggttg acattgaaga
      601 gacggtggag aacatggtga ggctgagacc ggaatacatc cgtatctatc ccctggtgat
      661 cctcaggaac acgcccttgt acaggcttca cgaaacggga gggttcaccc ccattgcctt
      721 cgacgaggcc ctcgagaggg cggcgctgat ctaccgtagg gccctggaag agggtataga
      781 cgtggtcaac gtggggctca cggacaacga actggtcagg gagatggttg ccggggggtt
      841 ctaccatccc gcttttggca gtctcgtgca gtcacgcata ttccgcgacg cccttggatc
      901 cgttctcggg gagatcgagg gcccgaagga ggtcacggtt gtcctccaca ggaacgacat
      961 acctctcctc gtaggataca ggagggagaa cctgaagcat ttcgcgtctc tgggtatcgc
     1021 ccttcgctgg gagccgtcgg gggccgaacg gggccgtttc gaggttgtaa gtggagggga
     1081 gagggtgacg gggagaatga tacagagaag ataatgacca aattattggt cattatcttc
     1141 ttctttggtc attgtgattc ggtccttggt tattttcttt cagaaccatg ttctgatgaa
     1201 tttgaaaaga tggtcgatct tgaatatcct cgagggttcg agagcgaggg ccttccggta
     1261 gtactcccga gcaagcttgt cgtccccgga acggtagaac cgccggaaca actccacgta
     1321 gcggttggcg gcgaagggtc ttcttatgtg ttccagcctt ccggcgaaat aggggtcgtc
     1381 gaagatcgcc tcgatgaggc ccgcaccctg cgttctgaga tttgctatgt tgtggcgaag
     1441 actgtccgga tggctgaagg tgacggctgt cggtttcgag aggaaacagc ccttcctgtc
     1501 gaagaacacc ttggaaaaga agaccaggtc ctcgccgttg ttcagatccg tggcaaactc
     1561 gaactttctc gcgtggcttc ccctcacgag gacggcggat gtcgtgtagg gtatccgttt
     1621 gaggagaagt gcctggcaca actccctgga aacgatgtca cgcggtcccc tgtagggccg
     1681 gaaggatacc ctgccggcat cgtccaccga tgaggcggtc gtcgttacga aatctatgtc
     1741 ggggttctcg agcatgacgg tgacccgtgt ttccacagag tcgtcggtga ggtaatcgtc
     1801 tgcgtccaga aaggcgatga aatctccctt tgccatcctg agccccttgt ttctggcggc
     1861 agaaaccccc tggttttcct gataatgata gacaaccctc tgatcagcga ttttccttac
     1921 tatttctccc gttgcgtccg tcgaaccgtc atcgatgact atgacctcga ggttgccgga
     1981 ggattgtctc aggcacgatc ctatggccct gtgcacatat gtcccatagt tgtacgcggg
     2041 cacgatgatg gatacgagtg gctgctcctt caagaccgtc taccgccttc ccttctcttc
     2101 gaatgccccc aggagcactg cctccatctc gtcggcgttg cgttccatgg tgaaggactc
     2161 catcttcttc cgcgcgttcc ttcccatgac ttcctgtctc accgggtctg ttagggcgca
     2221 gatggcggcg ctcagagcct cgatgttgtc tgcctcgtcg atgacgaagc cctcgtcggg
     2281 agagacgaag gcgcatgccc cgttccaccg ggtggtgatg atgggaaggc ccgatgccat
     2341 gccctccatc gtggtcagcg agcaggcgtc gtagtaggtg gggtgtgcga gcacgtgggc
     2401 ctcacggtag acctcttccg gcgcggagcg ctcccccgtg aaggtgacgc ggtccgcgat
     2461 gcccgccgcg tcgatgaggc cccggtacct ctccttcttc ccccggccca tcacggtgag
     2521 ccgcacgtcc tttccctttt ccaccgccct tcccatggct gcgatgaggg gggagagtcc
     2581 cttgagcctg aagttccccg cgcagaagag gatcgtcgtg gggcctgccg gccgctccgg
     2641 agccggctta aagcggtccg tgtcgacacc gttgtagacg acgtcgaagg tgttcctgtc
     2701 aatgccgtag tgcgccgaca tgtggtcccg gaccatgtcg gatatggcca ctatcctcgt
     2761 gaacctgtgt tttttcacgg agtatccctc tatccagcgc tggatggcct ggttgaggct
     2821 gttcctcaga aggaacgctt tcatgccggc gcctcccggc ccatagctca cgatctccct
     2881 ttccatccag acgctctgca cccctccatg gctctggtaa acgtcgaggt cgagaatgtt
     2941 gccgtagccc agcatgatgt cgggacggat cctgtcgagg gcctgtctgt gcgtcagggc
     3001 gaaggacagg ttccggcgcc accgggggaa ggaattcgtg cgaaggcgta tgagcctgac
     3061 accttccgtc tcgacgccgc ggccgcagaa gacacagatc tcgtgacctt tgccggcaag
     3121 tctccgggca aggtcaaagg tgtagcgttc ggcgccgcct ttcttggggt cgaaattgta
     3181 tatagcgaga ccgatcttca tcctcagtca ctccgcgtcg atgaggggtc cggcgaggga
     3241 ggtcacgcgc gcctgccact ggtgaggagg aaatcgtaga attgcttcat ggcctttgcc
     3301 tggaggcgaa agctccactc gctgtcggcg cggttccggg cgttctcccc gaaggcgcgc
     3361 cgtctggcgg cgtcgctgac gagaagctcc atcttgccgg cgaggtccgc cgcgtcccag
     3421 gtcgtcacgt acccggtcct gccgtcctcc acgaggtccg ggagcattcc cctgtctgac
     3481 acgatggcgg gccgccccat gctgagcacc tccctgagcg cccggccgct gccgtcgctt
     3541 cccgcgcgca tcatgatgta aagatcgaag gtgtgtatca tcgagaagta gtcgtcgatg
     3601 cggtatccgg cgatcacgac gtctttctcg atgccgagtt ccttgagtgg tttcatgacg
     3661 ctcgtttcta tttgggaact gcggcccacg atgacgagct tgaccctgtc cacacgtgac
     3721 ctcagcatgc cgaaagcgct gaggatgaca tcgtagccgc ggtcgggctt gagtctcccg
     3781 atgacgccga tgacccgttc atcgggagcg atcccgagtt cggcgcgcag gtcctttaag
     3841 ggcccttcgt aaggcttgac ccccggcggg atgacgcgaa ggcgctctcc gggataggcg
     3901 aagctctccc tgtcgcgcgc ggcgagcctt tcgctgtagg ttaccagccc gtccgttctt
     3961 gccatggccc aggccatgaa gatgttcgcg gggagcccgt cccgcttgtg gtccgtcctc
     4021 acgaggaggg gccgtgccct ggagaaggaa agcgccgtga cggcggtgta gtaatcatgg
     4081 gaaagatggg tatggacgat gtcgaatttc tccttcaaca cgtacggatt gaaggagatg
     4141 aggtcatgcc accagtcgcg ggcggagaaa tacctgttga gcctcaaacc ctcatagcag
     4201 cgaagccctc ttttgcctgc ctccctgtcg acggtccttt cgcgaaaatc cgcgggcgac
     4261 ttcctgcagg cgaggacgac gtcgacgccc tcgttcccga ggttctcgca gagggagagc
     4321 accggttcgg cgggccccgt ccatttccag tcgctgaaga ggtgaagtac cttcatcttc
     4381 cgtagaattc accgatcgtc cggcatacga aaccgatctc ttcttcccga agcgaggggt
     4441 ggacgggcag ggagatgatc tgccgtgacg ccttttcggc tttcgggaag gagccctcgc
     4501 catacccgag gtaactgtag accttttgaa ggtggatggg tgtcggatag tggacgaggg
     4561 tggtcacgcc cttttcgctc aggtatgccc tcaatgcctc cctctcttca acggcgatga
     4621 cgtagaggtg atagacgtgg aaggcttgag gtaccgtgac gggccggcga acgggcaggc
     4681 cttcgaggcc cctgtcgtac agcgtggcgt tgcgcctgcg cccttcgttc cagccgtcga
     4741 gatgggggag cttgacggaa agcagggcgg cctgcagttc gtcgagacgg gagacaaaac
     4801 cctgaatgtc gtgaacgtgc ctgtccgact ggccgtgcgc gcggagcatg agcattcttc
     4861 tgaagacgtc ttccctgtcg gtgacgacga tcccgccgtc gccatagcag ccgaggttct
     4921 tcgtgggata gaaactgaag gcggccacgt cgctcatggt tcccgcgttc cggtccttgt
     4981 accgggagcc gtgtgcctgg caggcgtctt ccatgaccct gaccccgtgc ctttcgcaga
     5041 tatcccgtat ctcgtccatc ctgcacggct gtccgtaaag atggacgggc acgcagagtt
     5101 ttatgtcctt ctccttgccg agaagctctt cgagcgcggc ggggtccatg ttgtaggtat
     5161 ccgcctctat gtcgcagagg cgagggacca ggcctccctc ggaaagggcc atcgccgtgg
     5221 cgatgtacgt gttgggcgtg gtgacgaact tgtccccttc cgtgagaccg agggcaagcc
     5281 cccctatctt tatggcatcg gttccgctcg cgacgccaac ggcgtacctc gtgcccacgc
     5341 aggcggcgaa ggcctcttcg aaggacttga cctccctgcc gaggatgtat tccccggagg
     5401 agagcacctt ttcgaatgtt ctcacaagtt ccgccttgat cccggcgtgg tctttttcta
     5461 tatcgaatat tctcacgttc acgatgcggt accgtccttt tcggtcatgg cacgcccgac
     5521 ggcttaccag tattccatcc tgtgcttccg gtagaaatcg atggtcctcg agatcccctc
     5581 atcgagggac gtcgccggtt gccagcccgt cgtcccggtg atgcgcgtta tgtcggcgta
     5641 ataatcaccg ggttccacct ccttgcgctc ctgggtgaac tcggtgaacc tcgttctccc
     5701 cgtcccggcg atagccacga tcttctcggc aagctcgaag aaactgacgg ggacgccggt
     5761 accgacgttg aagaccttcc cgtaggcctc ctccgcggcg gccaccttga gaatcgagtc
     5821 cacgaggtct tcgacgtaga ggaagtcccg gagtatcctg ccgtcaccga agacgggtat
     5881 ctcctcgtcg tcgagggcct tccggatgaa ccagttgaag accccgtact cgtcgtgggc
     5941 catctgatgg cgcggcccgt aggtgttggt gatgcgcagg cacacgcccc gtatcttgta
     6001 gacgtcgtca tagacgagga ccatcttctc cgccgtcagg ttcgtgacgg cgtagatgcc
     6061 cttggggttt gtcgggtggt cttcgtccac gggcagcctg acgctcgatc cgtactcgcc
     6121 ccgcgtcccg gcgaagatga ccctggcgtc cctgttgttg tggcgcaggg cctcaaggag
     6181 gacgagcgtc cccgtgcagt tgatctccag atcctgcagg gggttcttca tactgtccac
     6241 gtggttgacc tggcccgcca ggtggaagat gtagtccttc cccttgacga ggtggttcat
     6301 ggagagctga ttgcggacat cggagacgtt gaccgtaacc ctgtcggcaa tgtcgcgtat
     6361 gttgaagaga ttgccgccct ggcgcgggag catgttgtcg atgatggtga cccgcgcgcc
     6421 gagcctcacg agttctatgc agaggttgct gccgatgaag ccgagccctc cggtgacaag
     6481 gacgtctttg
//
LOCUS       DAOPOA010000032         6305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_13554_length_6305_cov_3.793920, whole genome shotgun sequence.
ACCESSION   DAOPOA010000032 DAOPOA010000000
VERSION     DAOPOA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13554_length_6305_cov_3.793920"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1218
                     /locus_tag="PKJ17_01300"
     CDS             55..1218
                     /locus_tag="PKJ17_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD74631.1"
                     /translation="MDYFLSEDQKSIQKLARRIAEEKVVPVRAELDETGQFPWEIMKH
                     CAETGLFGVSIPEEYGGMGGGSFENCIVVEELSKACLGVSVSYAASLLGAYPILLGGS
                     EEQKKKYLSQIASGSKLAAFGLTEANAGSDAQGIRTEAKKDGDHYILNGTKQWITNGG
                     EAEIYTVVAMTDRSKGGRGATAFILEKGMEGFTFGKKENKLGIRASATRELVFQDCKV
                     PKENVIGREGLGFILTMRTFDRTRPGIGAQAVGVAHGALEAAVQYAREREQFEKKIIS
                     FQAIQHILADMAIQVEAARALVYAVARYIDSNPRDFSKVSAMSKVFPSDVAMKVVVDA
                     IQVFGGYGYMKEYPVEKMMRDAKILQIYEGTNQIQRNIIGLELIKQSASKKRS"
     gene            1271..2209
                     /gene="fabD"
                     /locus_tag="PKJ17_01305"
     CDS             1271..2209
                     /gene="fabD"
                     /locus_tag="PKJ17_01305"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOD74632.1"
                     /translation="MKKTGMVFPGQGSQYIGMGRDLYDRFDYVRDMFATADRVLGFPM
                     TDLCFNGPEDELRQTYNTQPSLLLVGYAVHEVLRREAGIEPFLVAGHSLGEYTALTAS
                     GFFTFEEALAITRKRGLLMEEACPKGKGGMVALIGADMEKIGPVLAEISHDDYVAVPA
                     NLNSAEQVVLSGDAGALREAVERLKGTGYKKAVFLNVSGPFHSPLMQAAADRLKSELA
                     ALGHGELSVPVVFNVDATPGKDKGSVDDRLYRQMFSPVLWERCARRMADEGVEIFIEA
                     GPGKVLSNLVKRIVPDIPCVNAEKFEEVESIKGLMA"
     gene            2206..2931
                     /gene="fabG"
                     /locus_tag="PKJ17_01310"
     CDS             2206..2931
                     /gene="fabG"
                     /locus_tag="PKJ17_01310"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOD74633.1"
                     /translation="MNGTVTIITGAAQGIGRAIAELVAGKGGDVAIFDVVDGSGACEA
                     IRSKGRRCEFYTVDVSDSQAVQGAVDQVVKDLGKIDNLVNNAGITADKLLLRMKEEDW
                     DRIMAVNLKSVFNCTKAVVRHMLRSGGSIVNISSIAGVMGNAGQANYAASKAGIIGFT
                     KSVAREYAERSIRVNAVAPGFIRTKMTDALDEKTKESMHAAIPLKRLGEPEDVANVVY
                     FLLSKYGSYVTGEVINVNGGLYM"
     gene            3023..3262
                     /locus_tag="PKJ17_01315"
     CDS             3023..3262
                     /locus_tag="PKJ17_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOD74634.1"
                     /translation="MSVTEKVKKMIVDQLGVSESEVVPEAKFIDDLGADSLDIVELIM
                     ALEDEYSIEIPDEDAEKMETVGDAIRYIEDRLAGK"
     gene            3279..4520
                     /gene="fabF"
                     /locus_tag="PKJ17_01320"
     CDS             3279..4520
                     /gene="fabF"
                     /locus_tag="PKJ17_01320"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF004970.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOD74635.1"
                     /translation="MKRRVVITGVGLVTPLGVGIDSVWTEILNGRSGIAPLTRFDSTQ
                     HDTKIAGEVKDFHAEEYVSAKEVKRMDLFIQYALAATKIAMGDSGLDISKEDAERIGV
                     IVGTGLGGLPTLEKYHSILLERGPGRISPFFIPMLIANEAPGHIAIQYGIKGPNLCIV
                     TACATGAHSIGDAMRVIEYGDADMMVAGGCEANLTPLTVGGFNAVKALSTRNSEPERA
                     SRPFDKDRDGFVVAEGAGIVILEELEHARKRGAKIYAELAGYGYNGDAYHITAPCPDG
                     DGFIRCINMALRDASLPADAVDYINAHGTSTDLNDQTESLAIKKVFGEKAYKVPVSST
                     KSMTGHLLGAAGAVEAIFTALSIRDQICPPTINYETPDPDCDLDYVPNKARKHTINVA
                     LSNSFGFGGTNCVLVLRRFEG"
     gene            4517..4966
                     /gene="rpiB"
                     /locus_tag="PKJ17_01325"
     CDS             4517..4966
                     /gene="rpiB"
                     /locus_tag="PKJ17_01325"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOD74636.1"
                     /translation="MKIAVGSDHAGFELKEYIKGILPPGEHEVADVGTHSPESVDYPD
                     FGLEVARRVAAGEADRGIIVCGTGIGMSIAANKVRGIRAALVNDLYTAAQSRRHNDAN
                     VLVLGGRVIGKGLAEEIVKTWLSTEFEGGRHLGRIRKIDDFEKNTDR"
     gene            4970..6217
                     /gene="glyA"
                     /locus_tag="PKJ17_01330"
     CDS             4970..6217
                     /gene="glyA"
                     /locus_tag="PKJ17_01330"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOD74637.1"
                     /translation="MKLDGKDTKVFELMRRELDREEYSLILIASENYVDEDVLATQGC
                     VLTNKYAEGYPSKRYYSGCAYLDEIESIAIERARTLFGVEHANVQPHSGSSANMAVFY
                     AVLERGDTVLGMDIAHGGHLTHGARPSFSGKWYRAVSYPVSAKNEMLDYDEIRGIARK
                     ERPKLIIAGASAYSKIIDFAKFREIADEVGAYLMADIAHIAGLVAAGLHPSPAGHAHF
                     ITTTTHKTLRGPRGGLVMCNADLAKKIDSAVFPGIQGGPLMHVIAAKAVALRQAMTEE
                     FKEYQRQIIANAKHMAKRMADLGFRIVSGGTDNHLFLVDLTPKNVTGNDAQIALEASG
                     IMVNKNLIPFDTKTPGVTSGIRIGTPAVTTRGMKEKEMEVICEMIDSVLKRPGDTSFM
                     DGTRERVREMCKGFPFYSRIYDI"
BASE COUNT         1495 a         1734 c         1974 g         1102 t
ORIGIN      
        1 ggcgaggcca tgcaggacta cgaatcgttt cagaaagtaa aggagaggta aggtatggat
       61 tattttctat ctgaagacca gaagagcata cagaagctgg cccgcaggat cgcggaggaa
      121 aaggtcgttc ccgttcgcgc tgaactcgac gagacaggac agttcccctg ggagatcatg
      181 aagcactgcg ccgaaacggg gctcttcggt gtcagcatcc ccgaggagta cggcggcatg
      241 ggaggaggat ccttcgagaa ctgcatcgtc gtcgaggagc tgagcaaggc gtgcctcggc
      301 gtgtcggtga gctacgcggc cagcctgctc ggtgcctacc ccatcctgct cggcgggtcg
      361 gaggagcaga agaagaagta cctgtcccag atagcgagcg gatcgaagct ggccgccttc
      421 ggactcaccg aggctaacgc cggcagcgac gcccagggca tcaggacgga ggcaaagaag
      481 gacggcgacc attacatcct caacgggacg aaacagtgga taacgaacgg cggtgaggcc
      541 gagatctaca ccgtcgtcgc catgacggac cgttccaagg ggggccgggg cgccaccgcc
      601 ttcatactcg aaaaaggcat ggaaggattc actttcggta agaaagagaa caagttaggc
      661 atcagggcat cggccacccg ggagctggta tttcaggatt gcaaagtccc gaaggaaaat
      721 gttataggta gagaaggtct gggtttcatc ctgacaatga ggacttttga caggacccgc
      781 ccagggatag gtgcgcaggc ggtgggggtt gcccatggcg cactcgaggc agccgtacaa
      841 tacgcgcgtg aaagggagca gttcgagaag aagatcatct cctttcaggc gatacagcat
      901 atccttgccg atatggcgat acaggtggag gcggcacggg cactcgtgta cgccgtggca
      961 cgatacatcg acagcaaccc cagggatttc tccaaggtgt cggccatgag caaggtgttt
     1021 cccagcgacg tggccatgaa ggtcgtcgtc gatgccatac aggtcttcgg cggatacggc
     1081 tacatgaaag aataccccgt cgagaagatg atgcgcgatg ccaagatact ccagatatac
     1141 gaaggcacga accagataca gaggaacatc atcgggctcg agctcatcaa gcaatcggca
     1201 tcgaagaaaa gatcgtaacg tgagatactg atctcgcgag gaagagaatc cacacggcga
     1261 gagaggatag atgaagaaaa caggtatggt atttccggga cagggttccc agtacatcgg
     1321 catgggcagg gacctctacg acaggttcga ctatgtccgc gatatgttcg ccaccgccga
     1381 cagggtgctc ggtttcccca tgacggacct ttgcttcaac ggtcccgagg atgagctgcg
     1441 ccagacgtac aacacgcagc cgtcgctcct tctcgtgggc tatgccgtgc atgaggtctt
     1501 gaggagagag gcgggcatcg agcccttcct ggttgcgggc cacagcctcg gcgagtatac
     1561 ggcccttacc gcgagcggct ttttcacctt tgaagaagcc cttgccataa cgaggaaaag
     1621 aggcctcttg atggaagagg cctgcccgaa aggcaaaggc ggcatggtcg ccctcatcgg
     1681 tgccgacatg gagaagatag gccccgttct cgcggagata tcccacgacg actacgtggc
     1741 ggtccccgcc aatctcaact cggcagagca ggtcgtcctt tccggcgacg ccggcgcgct
     1801 cagggaggcc gtggagagac tgaaagggac gggatacaag aaagcggtct tcctcaacgt
     1861 ctcggggcct ttccattcgc cgctcatgca ggcggccgcg gaccgtctga agagcgagct
     1921 tgcggccctc ggccacgggg agctttcggt ccccgtcgtg ttcaacgtcg acgcgacccc
     1981 cgggaaagac aaagggtcgg tggacgacag gctctaccgt cagatgttct cccccgtgct
     2041 ctgggaaagg tgcgcgcgca gaatggccga cgagggcgtg gagatcttca ttgaggcggg
     2101 tcccgggaag gtcctttcga accttgtcaa gaggatcgtc cccgacatcc catgtgtgaa
     2161 cgcggagaaa ttcgaagagg tggaatcgat aaaggggctt atggcatgaa cggaacggtg
     2221 accatcatta caggtgcggc acagggcatc ggcagggcga tcgctgagct cgtcgccggg
     2281 aagggcggcg acgtcgcgat cttcgatgtg gtcgacgggt ccggggcctg cgaggcaatc
     2341 aggtcgaaag gccggcgctg tgagttctac accgtggacg tgtccgactc ccaggcggtc
     2401 cagggagctg tcgaccaggt cgtgaaggac ctggggaaga tcgacaacct cgtcaacaac
     2461 gcgggcatca ccgcggacaa gctccttctg cgcatgaagg aggaggactg ggaccggatc
     2521 atggcggtga atctgaagag cgtcttcaac tgcacgaagg ccgtcgtcag gcacatgttg
     2581 aggtcgggag gcagcatcgt caatatctct tccatcgccg gcgtcatggg aaacgcggga
     2641 caggcaaact acgcggcaag caaggcgggc atcatcggct tcacaaagag cgtggccagg
     2701 gaatacgcgg agcgttccat aagggtcaat gccgtcgccc ccgggttcat cagaacgaag
     2761 atgacggacg ccctcgatga gaagacgaag gaatcgatgc acgcggcgat cccgctcaaa
     2821 cgtctcggcg agcccgaaga tgtcgctaac gtggtatatt ttcttctttc gaaatacggc
     2881 agctacgtaa cgggggaggt gataaatgtc aacggcgggt tgtatatgtg atcaacgcgt
     2941 gacccggggg agcaggtcgt tttgacgcga cggtcttccg ggagtttgaa ggaagctttc
     3001 aataaaacga ggaggtggct taatgtcagt aactgagaaa gtaaaaaaga tgatcgtgga
     3061 tcagctcggg gtaagcgagt cggaagtcgt tccggaagct aagttcattg atgacctcgg
     3121 tgcggattct cttgatatcg ttgagcttat catggcactg gaagacgagt acagcattga
     3181 gatccccgat gaggatgcgg agaagatgga aaccgtgggg gacgcgatca ggtacataga
     3241 agatcgcctg gcgggcaaat aatccagcga ggtgtcgttt gaaaagacgg gttgtcatca
     3301 ccggtgttgg tctcgtaacg cccctgggag tgggaataga cagcgtctgg acggagatcc
     3361 tcaacgggag atccgggatc gcaccgctaa caagattcga cagtactcag catgatacca
     3421 agatagccgg cgaggtgaag gacttccatg ccgaggagta tgtctcggcc aaggaggtca
     3481 agcggatgga tcttttcatc cagtacgccc tcgccgccac gaagatagca atgggggact
     3541 ccggtctcga catctccaag gaagatgccg aacggatcgg ggtgatcgtc ggaacgggcc
     3601 tcggcggcct gccgacgctc gagaaatacc acagcatcct gctcgaaaga ggcccggggc
     3661 gcatctctcc tttcttcatc cccatgctca ttgccaacga ggccccgggg cacatcgcca
     3721 tccagtatgg gataaaaggg cccaatctct gcatcgtgac cgcctgcgcc accggcgctc
     3781 attccatagg cgacgccatg agggtcattg agtacggtga cgccgacatg atggtggcgg
     3841 gaggctgcga ggcgaacctg acgccgctca ccgtgggcgg attcaacgcg gtgaaggccc
     3901 tctccacgcg caacagcgaa cctgaaaggg cttcgcgtcc cttcgacaag gaccgcgacg
     3961 gtttcgtcgt ggccgaagga gcggggatcg tcatcctcga agaactcgaa cacgcccgga
     4021 aaaggggagc gaagatctac gccgaactcg ccggttacgg gtacaatggg gacgcctacc
     4081 acataacggc cccctgcccc gacggagacg ggttcatccg atgcatcaac atggcgctcc
     4141 gcgacgcctc gcttccggcg gacgcggtgg attacatcaa cgcccacggg acctccacgg
     4201 acctcaacga ccagacggaa agcctggcca taaagaaggt ctttggcgaa aaggcctaca
     4261 aggtccccgt gagttccacg aaatccatga cgggtcacct tctcggcgcc gccggcgcgg
     4321 tagaggccat cttcaccgcg ttgtcgatca gggaccagat ctgccctcca acgatcaact
     4381 acgaaacacc agaccccgac tgcgatctcg actatgtccc caacaaggcg cgcaaacaca
     4441 ccatcaacgt ggcgctctcc aactcgttcg ggttcggtgg taccaactgt gttctcgtcc
     4501 tcaggaggtt tgaaggatga agatagcggt cggctcggac catgccggtt tcgagctgaa
     4561 ggaatacata aaggggattc tccctcccgg agagcacgaa gtggcggacg tgggaacaca
     4621 ctccccggaa tccgtcgact accccgattt cggcttggag gtcgcgagac gtgtcgcagc
     4681 cggtgaagcg gacaggggaa taatcgtttg tggaaccggg atcggaatga gcatcgcggc
     4741 gaataaggtc agaggcatcc gtgccgctct tgtcaacgat ctctacacgg cggcccagag
     4801 ccgcaggcac aacgacgcca atgttctcgt cctcgggggc cgggtaattg gaaagggtct
     4861 cgccgaagag atagtgaaga cgtggctctc caccgagttc gaagggggca ggcacctggg
     4921 caggatcagg aagatagacg atttcgagaa aaacacggac aggtaagaca tgaaacttga
     4981 cggaaaagac acaaaggtat tcgagttgat gagacgcgaa ctggaccgcg aggaatacag
     5041 tctcatcctc atcgcctccg agaactatgt agacgaggat gtcctcgcga cacagggatg
     5101 cgtcctcacg aacaagtatg ccgaagggta tccgtcgaaa agatattaca gcggctgcgc
     5161 ctatctcgat gagatagaaa gcattgccat agagcgcgcc aggacacttt tcggggtgga
     5221 gcacgccaac gtccaacccc attccggctc gtcggcgaac atggccgttt tctatgccgt
     5281 ccttgaacgc ggcgacacgg tcctggggat ggacatcgcc catggcggcc acctgaccca
     5341 cggcgcgagg ccgagcttct cgggcaagtg gtaccgcgcg gtgtcgtacc cggtatcggc
     5401 gaagaacgag atgctcgact acgacgagat acggggcatc gcccgcaagg aaagaccgaa
     5461 gcttatcatc gcgggagcca gcgcctattc gaagatcata gacttcgcga aattcaggga
     5521 gatcgccgat gaggtggggg cctacctgat ggccgatatc gcccacatcg ccggccttgt
     5581 cgctgccggc cttcatccga gcccggcggg acacgcccat ttcataacta ccacgacgca
     5641 caagaccttg aggggcccgc ggggcgggct cgtgatgtgc aacgccgacc tggcgaagaa
     5701 gatagactcc gccgtcttcc ccggaatcca gggaggacct ctcatgcacg tcatcgccgc
     5761 caaggcggtg gccctgagac aggccatgac ggaggagttc aaggagtacc agcgccagat
     5821 catcgcgaac gcaaagcaca tggcgaagcg catggccgac ctcggcttcc gcatcgtgtc
     5881 cggaggcacc gacaaccatc tcttccttgt cgacctcacc ccgaagaacg tgacggggaa
     5941 cgatgcccag atagccctcg aggcgagcgg catcatggtc aacaagaacc tcatcccctt
     6001 cgacaccaag acccccggtg tcacgagcgg catcaggata gggacacccg ccgtcaccac
     6061 gcgcggtatg aaggaaaagg agatggaggt catctgcgag atgatcgatt ccgtcctgaa
     6121 gcggcccgga gacacgtcat tcatggacgg cacgcgagag agagtgaggg agatgtgcaa
     6181 gggctttccc ttctattcgc gcatatatga catatgaaga accatcgtcc tgactggggt
     6241 tcctacttca tggaaatagc acggatcgtt tccatgaggt cgacttgcct gcgccgaaac
     6301 gtggg
//
LOCUS       DAOPOA010000033         6141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_14028_length_6141_cov_3.609760, whole genome shotgun sequence.
ACCESSION   DAOPOA010000033 DAOPOA010000000
VERSION     DAOPOA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14028_length_6141_cov_3.609760"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..3922
                     /locus_tag="PKJ17_01335"
     CDS             188..3922
                     /locus_tag="PKJ17_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD74638.1"
                     /translation="MRSGHPYRLSSRSYLLFILAFLFFLDGFALWLAITVDHDRTLEQ
                     AHAVLERTAVSLQERMERVVFSTEAILRNRASRIREQGMGRAVASRKEWEHFRRGAQS
                     LPGPGQLWLLDHRGNLVMSSTEYPSRRANYREKEYFSDQTDRAVDLYIGPITKDDIVK
                     NYSFTITKRIEGNDGTLLGVVVAGIETGIFADFLRRISPGEGGTVAVFRGDGALVLRQ
                     PMEEEYIGKGFSHLALFEQDFEASPWGSFRTDKAIDGVGRIIGYSKIVGLPLIAATTL
                     PIDSVLAGWRERVGIYSIVAIIAFMALVALSLVIRRTTSREEKERTRQLAEINASLQA
                     QIAERIRAQAALKESEKRWATTLASIGDGIIATDVAGRITFMNAVAEALTAWRSMDAP
                     GKPVGDVFTVIDEDTRQAIRDPVARVLEAGATVGLSGHTILIRRDGRELPIDDSGSPI
                     LDSEGNMTGVVLVFRDIAGRRAAEKEITDSRRDLSRAQAVAHIGSWRLRVRERELTWS
                     DETYHIFGVIPGTPVTYEMFLAMVHPDDRETLIESWEGALAGAYYDVTHRIVVGGHIK
                     WVRAVAELEFDEEGTLLAGFGTVQDVTEQKRMEQALQVAHDELELRVEERTAEIRRQA
                     ELLDLANDAIIVTDTRGRMAFWNTGAVHTYGFTRKDMAGIMNHHTVLRTRFPIPVENI
                     LAIVESSGRWEGELTQTTKEGRRIVVLSRWAMRRGHSGRPPEIMEINTDITTRRQAEE
                     ALRLASVYNRSLIEASLDPLVTIDLEGKVSDVNSATELITGYSRADLIGTDHADCFTQ
                     PERARAGYRLVLEKGLVRDYPLEIRHRSGRSTSVLYNSSLYRDETGKIIGVVATARDI
                     TEKNRIERHLRETHKMEAIGTLAGGIAHDFNNIIAGIMGFAEMVLDDIPEDTPAHRRL
                     GYVLKGAHRGRDLVKQILAFSRRSEQEKKVISLDQIIDEVVTLVRATLPATIEIRRSN
                     TARSDLVSADPSQIHQVVVNLCSNAAHAMSEKGGAIEISLSEEIVSSAEGSPHHDLSP
                     GSYVRLTVSDNGRGMEPATLERIFEPFFTTKPPGEGTGLGLAVVHGIVRDHDGVITAS
                     SRPGGGSSFSIFIPQIRTGEADEIERIEAALEGPESILVVDDEEFIVEMSTQRLQRFG
                     YRATGATSSAEAIELFRADPGGFDLAVIDHIMPDMTGLELAKALLAIDPGIRIILCSG
                     LNEPIPMERMRQAGIRDFFTKPADRNEFIRIVRNVLDSPP"
     gene            4087..4884
                     /locus_tag="PKJ17_01340"
     CDS             4087..4884
                     /locus_tag="PKJ17_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOD74639.1"
                     /translation="MKRYLIFVAAAMCLIFSGTAPASETLVASGHPEYAPIMWKEGNN
                     IVGAGPELVKLLFKDLNVTVKTPYSGDWSKVQQEAKEGRVDLIVGLYLTEDRKAFFQY
                     SNPYAKDPVVAFVAKGKGFPYSKWDDLIGKKGTTTVGDSFGQVFDRFLAEKLSVTRSA
                     TVEESFSKLLKGRADYFIYAMYSGLFEADKMGITNKVELLSKEICVENWYIGISKKSP
                     YVTYLPQINKKLDELINNGTVDRLIEKYGSQYRKSIAKKKGSPAKAK"
     gene            complement(5002..6015)
                     /locus_tag="PKJ17_01345"
     CDS             complement(5002..6015)
                     /locus_tag="PKJ17_01345"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HOD74640.1"
                     /translation="MAFTVTTSRSFLARCSVPLIVFVLLFSSIAVGSSAGAFEEDVSA
                     PLTPSFSVGGKIRYLFKSHTSYEFGNPFPPCQEPLSRLEFPLDTWWAGLDMRLSFPRF
                     SLGLETLTSVKQNAHGEFEDSDWDDDARPDEKTTYSTSQMRVAPSYMVRFDADLEVSD
                     WLGLPGWLKIRPLVGIRYQRFNLVSHDGIQYDLTGASGNTLLPGEGIRFKQTYWHYFV
                     GLRSVIDLSRPTGIPSLSLNLQADWAYVEGMNEDNHLFRPGRRFTYEDTYGEAWHCSM
                     GLKKELGEGFSVGLDGDYLTVSTTGNHRLLNRTFGIDMSFSNGVKVWSDQLSLSLTLE
                     YRF"
BASE COUNT         1379 a         1795 c         1871 g         1096 t
ORIGIN      
        1 caggggtatt gcctcaggag cacagaggtg gtaagtatgt ccataccacg tgccgccgaa
       61 cgacgggtga cgggacagac aggacccgtc ccttctctcc cccccgggtt ctccccttcc
      121 cttgcacctt cgtgatgcat gtgcgataat taggcagcag tcgatccccc cggtgaggaa
      181 cgcgatcatg agatcaggac atccataccg gctctcttca agatcatacc ttttgttcat
      241 ccttgccttc ctcttcttcc tcgacggttt cgctttgtgg ctcgccataa cggtggacca
      301 cgacaggacc ctcgaacagg cccacgcggt cctcgaaaga acggccgtct cactgcagga
      361 aagaatggaa cgcgtcgtct tctccacgga ggccatcctg aggaaccgtg cgtcgaggat
      421 ccgcgagcaa ggcatggggc gcgcggtcgc ctcccggaag gaatgggagc atttccgaag
      481 gggagcccaa tcgctgcccg gtccgggcca actgtggctc ctcgaccacc ggggaaacct
      541 tgtgatgagc tcgaccgaat acccttctcg tcgggccaac taccgggaga aagagtattt
      601 ctcagaccag acggaccggg cggtcgacct ttacatagga cccattacga aggacgacat
      661 cgtgaagaat tactctttca ccatcaccaa gcgcatagaa gggaatgacg gcacgctcct
      721 tggtgttgtc gtggcaggca ttgagacggg gatctttgcc gacttccttc gccgcatcag
      781 ccccggagag ggagggacgg tcgcggtctt ccgaggcgac ggggccctgg tcctcaggca
      841 accgatggag gaagagtaca tcggcaaggg cttcagccat ctggcccttt tcgagcagga
      901 tttcgaagcg agcccctggg gcagtttcag gaccgataag gccatcgacg gggtcggtcg
      961 catcattggg tacagcaaga tcgtgggcct gcctctcatc gcggccacga cccttcccat
     1021 cgattccgtc ctggccgggt ggcgtgagcg cgtcgggata tattccatcg tggcgatcat
     1081 agccttcatg gcccttgtcg ccctctccct cgtgatacgc aggaccacat cgcgggagga
     1141 gaaggaaagg acgcgccagc ttgccgagat caatgcgtcc cttcaggccc agatcgccga
     1201 gcggatccgg gcacaggcgg ccctcaagga aagcgagaag cgttgggcca caacgctcgc
     1261 cagcatcggg gacggcatca tcgcaaccga cgtggcgggc aggataactt tcatgaacgc
     1321 cgtcgccgag gcgctgaccg cctggaggag catggacgcc ccgggcaaac ccgtgggcga
     1381 tgttttcacg gtgatcgatg aagacacccg gcaggccatt cgtgatcccg tcgccagggt
     1441 cctcgaggcc ggtgcgacgg tgggtctctc ggggcacacc atactcatcc ggagagacgg
     1501 cagggagctg cccatcgacg acagcgggtc ccccatactc gactccgagg gcaacatgac
     1561 gggtgtcgtg ctcgtcttcc gggacatagc gggccgccgg gccgcggaga aggagatcac
     1621 cgactcgaga cgcgacctca gcagggcaca ggccgtggcc cacatcggaa gctggcgcct
     1681 tcgagtccgg gagcgtgaac tgacgtggtc cgacgagaca tatcacatct tcggggtcat
     1741 accggggacc cccgttacat acgagatgtt cctggccatg gtgcatcccg acgaccggga
     1801 aacgcttatc gaaagctggg aaggggcgct cgcgggggca tactatgacg tcactcaccg
     1861 catcgtcgtc gggggccaca tcaagtgggt gcgcgcggtg gcggaactgg agttcgacga
     1921 ggaaggaacg ctgctcgcag ggttcggaac ggtccaggac gtgacggagc agaaacggat
     1981 ggagcaggcc ctgcaagtgg cccacgacga gctggagctt cgcgtcgagg agaggaccgc
     2041 tgagataaga cgacaggcgg aactcctcga cctcgcaaac gatgccatca tcgttaccga
     2101 cacacgggga agaatggcct tctggaacac gggggcggtc cacacatacg gcttcacccg
     2161 caaggacatg gccggtatta tgaaccacca cacggtcctc cgcaccaggt tccccattcc
     2221 cgtcgaaaac atacttgcca tcgtcgaaag cagcggccgg tgggaaggcg aactgaccca
     2281 gacaacaaag gagggacggc ggatagttgt cttaagccgc tgggcgatgc gccgcggaca
     2341 ctccggcaga ccccccgaga tcatggagat caacaccgac atcaccaccc gcaggcaggc
     2401 cgaagaggcc cttcgcctgg caagcgtcta caacaggagc ctcatcgagg ccagtctcga
     2461 ccccctcgtc accatcgacc tcgaggggaa ggtcagcgac gtcaattcgg cgacggagct
     2521 gataacgggg tattccaggg cggacctcat cgggacggac catgcggatt gcttcacgca
     2581 gccggaaagg gcacgcgcgg gctatcggct tgtcctggaa aaaggtctcg tgcgggacta
     2641 cccgctggag atacggcaca ggtccggccg cagcacgtcg gtgctgtata attcatccct
     2701 gtaccgggat gagacgggaa agatcatcgg cgtcgtggca acggcacggg acatcacgga
     2761 aaagaacagg atcgaaaggc acctgaggga aacgcacaag atggaggcca tcggcaccct
     2821 cgccgggggc attgcccacg atttcaacaa catcatcgcc ggcataatgg gcttcgccga
     2881 aatggtcctc gacgacatcc ccgaggatac tccggcgcac cggaggctgg gctatgtcct
     2941 gaaaggggcg catcggggcc gtgacctcgt caaacagatc ctcgccttca gccgcaggag
     3001 cgagcaggag aagaaggtca tttcgctgga ccagatcatc gacgaggtcg tcacactcgt
     3061 gagggcaacc cttcccgcta ccatagagat caggagaagc aacaccgcaa gatccgacct
     3121 cgtgtccgcc gatccctcgc agatacacca ggtggtggta aatctctgct ccaatgccgc
     3181 ccatgcgatg agcgaaaagg gtggggccat cgagatatcc ctttcggaag agatcgtttc
     3241 gtccgcggaa gggtcgcccc atcacgacct ttcgccgggc tcatacgtga ggctcacggt
     3301 gagcgacaac ggacgcggca tggagccggc cacgctggaa cggatattcg agcccttctt
     3361 caccaccaag ccccccgggg aggggacggg actgggtctc gccgtcgtcc atgggatcgt
     3421 gagggaccac gacggtgtga tcacggcatc aagcaggcct ggaggaggct cgtccttcag
     3481 catctttatc ccccagatca ggacgggtga ggccgatgag attgagagga tcgaggcggc
     3541 cctggaagga ccggagtcga tccttgttgt cgatgacgag gaattcatcg tcgagatgag
     3601 cacgcagagg cttcagcgct tcggctaccg ggccacgggc gccacgagca gcgccgaggc
     3661 gatagaactc tttcgcgccg acccgggcgg tttcgacctc gccgtcatcg accacatcat
     3721 gcccgacatg accgggttgg agctggcaaa ggcgctgctc gccatagacc cgggcatccg
     3781 catcatcctg tgctccgggc tcaacgaacc catcccgatg gagaggatga ggcaggcggg
     3841 catcagggac ttcttcacga agccggcaga caggaacgag ttcatccgga tagtgcggaa
     3901 cgttctcgac agccccccgt agcaaacctc cccggatgtc cctgctccct tcccgggaat
     3961 gcggatcgta taccgcaaaa ccacaaaacg acagatatcg gcgaatttgt tttttaacga
     4021 gggctggatt gtggtagtat ttcttcactt tcggtaaggc gcattcttct gggaaagggg
     4081 tactgcatga aacgctatct tatctttgtt gccgcggcaa tgtgccttat attctccggc
     4141 actgccccgg cgtcggaaac actcgtagcc tcagggcatc ccgagtatgc acccataatg
     4201 tggaaagagg gcaacaacat agtgggcgcc gggcccgaac tcgtgaagct tctcttcaaa
     4261 gacctcaacg tcacggtgaa aacaccctac agcggggact ggagcaaggt ccagcaggaa
     4321 gcgaaggaag gcagggtgga cctcatcgtg gggctgtacc tgacggagga cagaaaggcc
     4381 ttctttcagt attccaatcc ctacgccaag gatcccgtgg tggcgttcgt ggccaagggc
     4441 aaagggtttc cctattcgaa atgggacgac ctcatcggga agaagggtac cacgaccgtc
     4501 ggcgacagct tcggtcaggt cttcgacagg ttccttgccg agaaactctc cgtcacccgc
     4561 tcggcgacgg tggaagaaag cttttcaaaa ctcctgaagg gccgtgccga ctatttcatc
     4621 tacgccatgt attccggcct tttcgaggcg gacaagatgg gaataacgaa caaagtggag
     4681 ctcctttcga aggagatctg cgtggagaac tggtacatag ggatctccaa aaagtccccc
     4741 tatgtgacgt accttcccca gatcaacaag aagctcgacg agctaatcaa caacggaacg
     4801 gtggacaggc tgatcgagaa atacggcagc cagtatagga aatcgatcgc taagaagaag
     4861 gggagccctg cgaaggccaa gtagcagggt agtgggggta ggaactcaca atccaggaat
     4921 gcgccgcgcg gctggtccat cgtgcattcc ctttggcaac gcggcgcatt ccctggacaa
     4981 acgtcatgaa gagatctccg atcaaaagcg gtattccagt gtgagcgaca ggctcaactg
     5041 atccgaccac accttgaccc cgtttgaaaa actcatgtcg atgccgaagg tccggttcag
     5101 caggcgatgg ttgcccgtgg tggacaccgt caggtagtcg ccgtcgaggc ccactgaaaa
     5161 accctcaccc agttcctttt tcaaccccat cgagcaatgc caggcctcgc cgtaggtatc
     5221 ctcgtacgtg aaccgcctgc ccggcctgaa caggtgattg tcctcgttca tgccttccac
     5281 gtaggcccag tcggcttgca gattgagact cagggaaggt atgcccgtcg gccgcgaaag
     5341 gtcgatgacg gagcgcagac cgacgaaata gtgccagtac gtctgcttga aacggatgcc
     5401 ttctccaggg aggagggtgt ttcccgacgc tccggtcaga tcgtactgta taccgtcgtg
     5461 gctgacaaga ttgaacctct gataccgtat ccccacgaga ggccttatct tcagccagcc
     5521 gggcaggccg agccagtcgg agacctctag gtccgcgtcg aaacgcacca tgtagctggg
     5581 agcgacacgc atctgcgatg tgctgtaggt ggtcttttcg tcgggacggg catcgtcgtc
     5641 ccagtcggag tcttcgaact cgccatgggc gttctgcttc acgctggtga gggtctccag
     5701 cccgagtgaa aagcggggga agctcaatct catgtcgagc ccagcccacc aggtatcgag
     5761 gggaaattcc agccggctga gcggttcctg gcagggggga aagggattgc cgaactcgta
     5821 ggaggtatgg ctcttgaaaa gatacctgat cttcccccca acggagaatg acggcgtcag
     5881 gggagcggaa acatcctctt cgaaagcccc agccgacgac ccgaccgcga tagaggagaa
     5941 caggagaaca aacactatca gcggaacgga gcatcgggcg aggaacgaac ggcttgtcgt
     6001 aacggtgaat gccatgtcgt tgaacgctca tgttaccaca tcatccccgg cgattgtcgt
     6061 ctttatttgc cgggagacaa tgaaaaatgt ttctccataa aggcaaatgt gctaaaaggg
     6121 gaaggacggg ttccggcaga t
//
LOCUS       DAOPOA010000034         6137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_14043_length_6137_cov_3.746465, whole genome shotgun sequence.
ACCESSION   DAOPOA010000034 DAOPOA010000000
VERSION     DAOPOA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14043_length_6137_cov_3.746465"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..2343)
                     /locus_tag="PKJ17_01350"
     CDS             complement(97..2343)
                     /locus_tag="PKJ17_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD74641.1"
                     /translation="MDIFRRRILAKLIIILIVAVSAISVASILYFDRRNRGTLERELE
                     DAVSNLGEIGRLAYAVPMWQMAKTEIAYINRAVLTNRNIVAVNIYDESGRFVDSLRKN
                     PDTMEVVDDVTPSIFIIPKGAEDIHKVYYAVFHHEFIVGFFEIFYAERYINAEILARR
                     FNLTVAFTLIGLCMIVVIFVVVKKMLIAPVLTLADVSRQITKDRDYSTKLKNRSNDEI
                     GVLYESIADMLREIERNEEELRRTRGYLGNIIESMPSMLVSVDRDGTVTQWNHATEVA
                     TGISSEHALGKNLWRITDFFDSFKEAIEKIAETNEAAHLYSHRFDREETRYKDVSIYP
                     LVADGIGGVVIRMDDVTELEKKESLLQQAQKMETIGTLASGIAHDFNNILGSIIGNVS
                     LLQYKLKSGAAIPAEELSEMMATIDKASSRAADVVKQLLTLSSRRETAFSPVDLNVVV
                     KNVLHICENTFDKVVVLEARYPETKAMVWADQVQMEQIVLNLCINASHALTIMREKGG
                     EKGGRIMVSLDKVKPDRGFLSRHPDAGPEPYWLLSVEDNGVGIPEEMLPKIFDPFFTT
                     KGAGRGTGLGLAVVYNIIRQHSGFIDVVSSPGVGTIFNCFLPCLQDAAREKGAGKEQG
                     PPRGEGVVLIVDDEAGMREVAGTILQELGYSVLLAEDGDEALGVFRERKDEIDMVLLD
                     VIMPRRSGRQVYSEMKKIDPGVKVLFASGYWQDGGDEDLSSLDDAHFIQKPYTLQAIA
                     EAVRKRMT"
     gene            complement(2440..3243)
                     /locus_tag="PKJ17_01355"
     CDS             complement(2440..3243)
                     /locus_tag="PKJ17_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOD74642.1"
                     /translation="MTRALVYACIIFSVVFSFSNAVFAAGAYPEKLTAVYTDWYPYTY
                     SEGNSAKGFEIEVFTAVVNRMGIEVVFERFPFKRCLKMLEEGRAHVLISLLKTPERRQ
                     YTVFPDEHLSVSRTSLFTRTDTRVRYRGSLADLKGYTIGVVAGFSYGTLFDNADYLKK
                     EEVQNTRRLISMVVSGRLPLGIENEAVIRGVAGSLGVAGSIRFLNPPVHSRKLYAGFS
                     RRLELRKLAEEFSRSLKDFKNSGEYRDILRKYDVSHSELTRQREKTSGS"
     gene            3441..4481
                     /locus_tag="PKJ17_01360"
     CDS             3441..4481
                     /locus_tag="PKJ17_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOD74643.1"
                     /translation="MKKRPVSLVCGFIVIIVVFVSGAIAGEKESSEEALKKFFDDQKT
                     RTGTITDNAAAMKDYVSSVRDVNARYKNGNTLLHYAANRGYLEVTALLLKKGADINAR
                     DKDGRTPLHEAMAYRRYDVARFLVENGADMTVKNKDGETPLISIVYMDNKKTAVDLVN
                     FFIAKGFNVSRSADAKLLNESIGRGHHDVAVILLEKGIAFDDSSLRGAASMGYEDIFV
                     TLLSRGADPRHKDILRAASASGNVGILKTLLEKGQRPTAEDVDLALYKGRRDAAVLLN
                     EALKRSGGQDVDLTARCRMKADPGPCMALFDAGYYDEIAGRCRSFTYGGCGGSAPFET
                     ESACKRICEDNK"
     gene            4530..4976
                     /locus_tag="PKJ17_01365"
     CDS             4530..4976
                     /locus_tag="PKJ17_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74644.1"
                     /translation="MKIEFTKKQFETLLKAVYLGARTANAYEDDLEGNEYVELQKYIF
                     GFARDFGIEKYADYDEKLKVAWPSATLEEDEVLNELIDRYDDFTFLDKLIYNLAGRDM
                     LDKHGPKALEKMSDDEFFKEEEVFAEKYRKEFAKNGIKNLSISKGK"
     gene            complement(4987..5235)
                     /locus_tag="PKJ17_01370"
     CDS             complement(4987..5235)
                     /locus_tag="PKJ17_01370"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HOD74645.1"
                     /translation="MADLIGYIAACFTTFSLLPQILRIRRLKEARDVSLFLPLMIVIG
                     SVLWSVYGILISSIPVIVANGVALIIALITVFFTIRYR"
     gene            complement(5252..>6137)
                     /locus_tag="PKJ17_01375"
     CDS             complement(5252..>6137)
                     /locus_tag="PKJ17_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOD74646.1"
                     /translation="RGRDIRKGDPVTDMRGYGTFNLPPGSWSDDSSLMLCTAQSLLEG
                     FDLGRMGDLFVSWFTAGLWTPHGQAFDVGRGTWQAISRMQLGTPAATAGGREEENNGN
                     GSLMRILPVALYCAAAGDDELLRIAHEASAVTHGHPRSMMACGVYCFLIAALLDGMPP
                     REAYLLAMEKMKRLYGGPPFSEELAHFTRLLSGDIETVPEAAIESDGYVVHTLEASLW
                     CLLGTDAYNSAVLRAVNLGWDTDTTAIVTGGLAGTYYGLEAVPLQWRDTLARKQEIEA
                     LFERFVEKMICPFAFSSG"
BASE COUNT         1376 a         1784 c         1687 g         1290 t
ORIGIN      
        1 tccgtgtggt tagtataccg caactttggt gtccactaaa ggcatcatag gtcaatcaac
       61 agcctgtcaa gcgattcaag cggctcaagc cagtctttat gtcatccgtt ttcttaccgc
      121 ttcggcgatg gcctggaggg tgtagggttt ctgtatgaaa tgggcgtcgt cgagggagga
      181 gaggtcctcg tccccgccgt cctgccagta gcctgacgcg aacagcactt tcacgccggg
      241 atcgatcttc ttcatctcgc tgtagacctg tcggcccgat ctcctgggca tgatgacatc
      301 gaggagcacc atgtctattt cgtcttttcg ctcgcggaag acccccagcg cctcgtcgcc
      361 gtcctccgcg aggagaacac tgtaacccag ctcctgaagg atggttccgg cgacctcacg
      421 catgcccgct tcgtcgtcga cgatgagcac aaccccctcc ccgcggggcg ggccctgttc
      481 cttgcctgct cctttctcgc gagcggcgtc ttgaagacag gggagaaaac aattgaagat
      541 cgtgcccaca ccgggagacg acacgacatc gataaagccc gaatgctggc gtatgatgtt
      601 gtagacaacg gccaatccca gccccgtccc tctccccgcg cctttcgtcg tgaagaaggg
      661 gtcgaatatc ttgggcagca tctcctcagg gatccccaca ccgttgtcct cgacggaaag
      721 gagccagtac ggttctggac cggcgtcggg gtgccgggac aggaatcccc tgtcgggctt
      781 gaccttgtcg agggacacca tgatcctgcc gcctttctcg ccgcctttct ctctcattat
      841 cgtgagggcg tgggaggcgt tgatgcaaag gttgaggacg atctgctcca tctgcacctg
      901 gtcggcccat accatcgcct tcgtttcggg atatctcgct tcgaggacca ccaccttgtc
      961 gaaggtgttc tcgcatatgt gaagcacgtt cttcacgaca acattgagat cgacggggga
     1021 gaacgccgtc tccctcctgc tcgagagtgt caggagctgc ttgacaacgt ccgcggcccg
     1081 gctgcttgcc ttgtcgatgg tcgccatcat ctccgaaagc tcttcggcgg ggatagcggc
     1141 gcctgatttc agcttgtact ggagaaggga aacgttcccg atgatgctcc ccaggatatt
     1201 gttgaaatca tgagctattc cgctcgccag cgtgccgatg gtctccatct tctgcgcctg
     1261 ctgcaggaga ctctctttct tctcgagctc cgtcacgtcg tccatcctga tgaccacgcc
     1321 gccgataccg tcggccacga gaggatagat cgacacatcc ttgtagcgcg tttcctcgcg
     1381 atcgaaccgg tggctgtaga gatgcgcggc ctcattggtc tccgcgatct tctctatcgc
     1441 ctccttgaag ctgtcgaaga agtccgttat ccgccacagg ttctttccca gcgcgtgttc
     1501 ggacgagatc cccgtcgcca cctccgtggc atggttccat tgcgtgaccg ttccgtcacg
     1561 gtcgacggaa acaagcatgg aaggcatgga ttcaatgatg ttgccgaggt agcccctcgt
     1621 tctgcgcagc tcttcctcgt tccgctctat ctcgcggagc atgtcggcta tgctctcata
     1681 gagtacgccg atctcgtcat tcgacctatt cttcagtttg gtcgaataat ccctgtcctt
     1741 cgtgatctgc cgcgacacgt ccgcgagggt caggaccggg gcaataagca tcttcttcac
     1801 gaccacgaat atgaccacga tcatgcaaag accgatgagc gtgaaggcca ccgtcaggtt
     1861 gaagcgcctg gcaaggatct cggcatttat gtagcgctct gcatagaaga tctcgaaaaa
     1921 acccacgatg aactcatggt gaaagaccgc atagtagacc ttgtggatgt cctcggcacc
     1981 tttcgggata atgaatatgg acggagtgac gtcatcgacg acctccatgg tgtcagggtt
     2041 cttcctcagg ctgtccacga atctcccgga ttcatcgtaa atgttgacgg caacgatgtt
     2101 cctgttcgtc aggaccgccc tgttgatgta ggcgatctcc gtcttcgcca tctgccacat
     2161 ggggacagcg taggcgagcc ggccgatctc tcccaggttc gacaccgcat cctctagctc
     2221 tctctccagc gtccccctgt tccttctgtc gaaatagagg atggatgcga cgctgatcgc
     2281 ggataccgca acgatcaata tgattatgag tttggcaagt atcctcctcc tgaatatgtc
     2341 cacgaatgtt tcctctctct accagccccg aaagacttcc cgtgcaggaa ccggtcagac
     2401 tgaccctctt ctattatttt cggcgaatcg taagaaaagt caactgccgg aagtcttctc
     2461 cctctgccgg gtcaattcgg aatgggaaac atcatatttc cgcagtatgt ccctgtattc
     2521 gccggaattc ttgaagtctt tgaggcttcg ggaaaattcc tcagccagtt tccggagctc
     2581 gagcctcctg gagaagcccg catagagctt ccggctatgg acaggggggt tgagaaaacg
     2641 gatcgagccc gcgacgccaa ggctgcctgc gacacccctg atgaccgctt cgttctcgat
     2701 gccgagaggc aacctgccgc tgacaaccat gctgatgagc cgtctggtgt tctgcacttc
     2761 ctccttcttc aggtaatcgg cattgtcgaa gagcgtgccg tagctgaagc ccgcgacgac
     2821 accaatggtg tatcccttca ggtccgccag ggaacccctg taccggaccc gcgtgtccgt
     2881 cctggtgaag agagaggtcc gggagacgct gagatgctca tcggggaata ccgtatattg
     2941 cctgcgttcc ggggtcttga ggagcgaaat gaggacatgg gcacgaccct cctcgagcat
     3001 cttgagacat ctcttgaaag ggaaccgctc aaagacgacc tcgatgccca tcctgttgac
     3061 gacggccgtg aagacctcta tctcgaaccc cttggcgctg ttgccctcgc tataggtgta
     3121 cgggtaccaa tccgtgtaga cagcggtcaa tttctcaggg taggcacccg cggcgaaaac
     3181 cgcgttgctg aaggaaaaga ccacggagaa gataatgcac gcgtagacca atgcccttgt
     3241 catgaaaact tctaccatat taccgccggg attgccaccc cgcgcccgtc gtcaagacgt
     3301 tgaggtcacc ccggagcaac tcctttctcg atacgcttgc cattttgcct cgcctgatat
     3361 tacaatctgt gatgaaaaaa acgcgcgggg gtccgtgggg gacagtggca ggcgcaccaa
     3421 atgaaggagt gacaaccaga atgaagaaaa gacctgtatc tctcgtatgt ggattcatcg
     3481 tcatcatcgt cgtgtttgtc tccggcgcaa tcgccgggga aaaggaatcc tcggaagaag
     3541 cccttaagaa attcttcgac gaccagaaga ctcggaccgg gacgatcacc gacaatgccg
     3601 ccgcgatgaa ggactacgtt tcttccgtca gggacgtcaa tgcccgctat aagaatggaa
     3661 acacattgct ccattacgcg gccaacaggg ggtacctgga ggtgacggcg ctgctgctga
     3721 aaaaaggggc cgacatcaat gcccgggaca aggatggaag gacgcccctg cacgaagcga
     3781 tggcctatcg ccggtacgat gtcgcccggt tccttgtcga gaatggcgcg gatatgaccg
     3841 tgaagaacaa ggacggcgag acgccgctca tcagtatcgt ctacatggat aacaagaaaa
     3901 cggccgtcga cctcgtcaac ttctttatag cgaaaggatt caacgtcagc cgctccgccg
     3961 atgcaaagct cctcaatgaa tccattggaa ggggccatca tgacgtcgcc gtcatcctcc
     4021 tggaaaaagg tatagccttc gatgactcct cgcttcgtgg cgcggcgtca atgggatatg
     4081 aggacatctt tgtcaccctt ctctcacggg gtgcggaccc gcggcacaag gatatccttc
     4141 gtgccgcctc tgcctccggg aacgtcggca tcttgaagac tctgctggaa aaaggccaga
     4201 gaccaaccgc ggaggatgtc gacctcgcgc tctacaaggg aaggcgggat gccgccgtcc
     4261 ttctcaacga ggcactgaaa agatcgggtg gtcaggacgt agacctcacg gcaagatgcc
     4321 ggatgaaagc ggaccccgga ccgtgcatgg ctcttttcga cgcgggatac tatgacgaga
     4381 tagcagggag gtgcagatcc ttcacctacg gaggatgcgg cggatccgcg cccttcgaaa
     4441 cagaaagcgc gtgcaagaga atatgcgagg ataacaagta acgggtcatg gaagacggat
     4501 actgacccag acccgacaag agggagaata tgaagatcga atttaccaag aaacagtttg
     4561 aaacgctgct gaaggccgta tacctggggg caaggacggc caacgcctac gaagacgatc
     4621 ttgagggaaa tgagtacgtt gaactccaga aatacatttt cgggttcgcc agggatttcg
     4681 ggatcgagaa atacgccgac tacgacgaaa agctgaaagt ggcctggccc tcggccaccc
     4741 ttgaagagga tgaggtcctg aacgagctta tcgaccgcta cgacgatttc acgttcctgg
     4801 acaagctcat ctacaatctc gcgggacggg acatgctcga caaacatgga cccaaggcgc
     4861 tggaaaagat gtccgacgac gaattcttca aggaagaaga ggtcttcgcc gaaaagtacc
     4921 ggaaagaatt cgcaaagaac gggataaaga acctctcgat atccaagggg aaatgagagg
     4981 ctcgccttat ctgtatcgga tcgtgaagaa aacggtgatc agagctatga tcagtgcgac
     5041 accgttcgcc acgatgacgg gaatcgagct gatgagaatg ccgtaaaccg accacagcac
     5101 ggaaccgatg acgatcatca ggggcagaaa aagcgagacg tccctggcct ctttcagtct
     5161 ccggatacgc agtatctgcg gcaaaagcga aaaggtcgtg aaacaagcgg ctatataacc
     5221 gatgaggtca gccatttcta tcttcttatc atcacccgga cgaaaaagca aaagggcaga
     5281 tcatcttctc cacaaaccgt tcgaacaagg cttctatttc ctgcttcctc gccaatgtgt
     5341 ctctccactg cagcgggacc gcttcaaggc cgtaataggt gccggcgagc ccgccggtta
     5401 cgatggccgt cgtgtcggtg tcccagccga gattcactgc cctgagcacc gccgagttgt
     5461 aggcatctgt cccgagcaga caccacaggg acgcctccag cgtgtggacc acgtagccgt
     5521 ccgactcgat ggcggcttcg ggaacggtct cgatatcacc ggagagaaga cgggtaaaat
     5581 gggccaactc ttccgaaaaa ggaggcccgc catacaacct tttcattttt tccatagcca
     5641 gcaaatatgc ctcgcgtggc ggcatcccgt caaggagggc cgcgatgagg aagcaataca
     5701 ctccgcaggc catcatcgat ctgggatgcc cgtgcgttac ggccgaagcc tcgtgagcga
     5761 tgcggagcag ttcatcgtct cccgctgccg cgcagtagag ggccacggga aggatgcgca
     5821 tcagcgagcc attgccgttg ttctcttcct cccgtccacc cgccgtcgca gccggtgtgc
     5881 cgagctgcat cctgctgatc gcctgccacg tccccctccc cacgtcgaag gcctgtccgt
     5941 gaggggtcca gaggcccgcg gtgaaccagc tgacgaaaag atcgcccatg cgcccgaggt
     6001 cgaacccctc aagaaggctc tgcgccgtgc agagcatcag tgacgagtca tccgaccagc
     6061 ttcccggcgg caaattgaag gtcccatatc cccgcatgtc cgtgacggga tcccccttgc
     6121 ggatatccct tcccctg
//
LOCUS       DAOPOA010000035         6085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_14199_length_6085_cov_3.263682, whole genome shotgun sequence.
ACCESSION   DAOPOA010000035 DAOPOA010000000
VERSION     DAOPOA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14199_length_6085_cov_3.263682"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKJ17_01380"
     CDS             <1..475
                     /locus_tag="PKJ17_01380"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOD74647.1"
                     /translation="VPLIADGGIKFSGDITKALAAGADTAMIGNLFAGTDETPGETVL
                     YQGRTYKVYRGMGSLEAMREGKTRDRYSIPEDAPESKIVPEGIEGRVPYRGSLSISVN
                     QLVGGLKAGMGYVGTRTIEELQKKRRFIRITSAALRESHVHDVIITKEAPNYRIE"
     gene            479..2038
                     /gene="guaA"
                     /locus_tag="PKJ17_01385"
     CDS             479..2038
                     /gene="guaA"
                     /locus_tag="PKJ17_01385"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOD74648.1"
                     /translation="MDSHHRDYHDELILILDFGSQYTQLIARKVRELGVYCEIYPYNL
                     DLDAIRSMKPRGIILSGGPASINEKNAPICDRAVFELGVPVLGVCYGLQLLATSFGGK
                     VEKAPKREFGKAHLYIEKADRFLDGVKDGDIVWMSHSDKVLTLPRGFVALARSDNSSY
                     AAVRNMNGTVYGVQFHPEVHHTPKGKRILKNFLYKICRLKGLFSPQSFVELATEKIRR
                     EAGSEKVICALSGGVDSSVVAALIHRAIGDNLRCVFVNNGVLRKNEAEEVIAAYRDIL
                     HLNLKYVDAGEAFLKALKGVKDPERKRKIIGRLFIRIFEEEARDGGSVRYLAQGTLYP
                     DVIESVSFKGPSATIKSHHNVGGLPKRMKMTLIEPLRELFKDEVRVVGRELGVPEYIV
                     GRQPFPGPGLAVRIIGEVTEERVRVLQEADSIIRQEIEHNPSFKHIWQSFGILIPVKT
                     VGVMGDERTYANVIAVRVVESEDAMTADWARLPYKTLDTVARRIINEVADVNRVVYDI
                     SSKPPSTIEWE"
     gene            2045..5503
                     /locus_tag="PKJ17_01390"
     CDS             2045..5503
                     /locus_tag="PKJ17_01390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOD74649.1"
                     /translation="MPDFVHLHLHTQYSLLDGAIRFDRLFNAARDFGMPACAITDHGN
                     MFGAAEFYLEANQRGLKPIIGCEAYIAPGSRFDQKRVKGEDVAYHVVLLAMDNRGYQN
                     LLKLISLAHLEGFYYHPRIDREILSQYHEGLICLTACIKGEIPNAILRGTDEMVRNHV
                     DYYLSLFGDRLFFELQDNGLEEQRIVNERLIELSSHYGVPIVATNDCHYLRREEAKAH
                     ELLLCIQTGKTINDKDRLSFSTDQFYFKSPDEIALAFSRYPEALANTMRVAEMCNVTI
                     DTNTYHFPEFHPPAGMDLDEYFEKLARQGFEKRMPEIRAAYDSFGEELLDQYRKRFDY
                     EAGVIRKTGFSGYFLIVADFINYAKTHGVPVGPGRGSAAGSLIAFCLGITDIDPIKYD
                     LIFERFLNPERISMPDIDVDFCRKGRDRVIEYVTNKYGKDNVAQITTFGTMKSKAAVR
                     DVGRALGMPYAEVDKIAKLITTADRGIERAINDEPEVRELYQRDDRVRELLDNACVVE
                     GLARHASTHAAGIVIANKHLSEYLPLYRGKHDETVTQYDMKMIEKIGLIKIDFLGLET
                     LTLMDTIVKLLKAEGIEIDLARLPLDDTKTYELLSSGNTSGVFQLESRGMRDLLTKLR
                     PSQFDDIMPLIALYRPGPLKSGMVDEFIKRRNNPSLVAYETPKLKDILHDTYGVIIYQ
                     EQIMKIASVLAGFSMKDADALRKAMSKKIPEQLEKYKQQFVDGAVANDVPKDVAEKIY
                     DVIQRFGEYGFNKSHSTAYGYIAYQTAYLKAHYPIHYFAAMLTTEVGDTDKLVKYVGE
                     CRESGIEILPPDINMSQSDFTIVDSKIRYGLSGVRNVGEAAIESIIQAREEAGGFKSF
                     VHFCSVIDSRKVNKKVIESLAMAGCFDSLGLNRSQVLYLAQEMLDKLTKRDTKNNHKQ
                     GSIFGEAEVAETTVSFDIPAMDELSHEEILLGEKESLGFYFSKHPLKPYEKLIAELTP
                     YDSQSLKEADTEQDVSMAGIVSVLKEITTRRGDRMAYVTLEDTKGTVETICFPDLYGK
                     NHFVIQSGKPLMVTGSLERSEDGSARIKAKAVALLEALTGELLKTVRIRIHCEVIRKE
                     DLRVLKDILFSLRGRSGVLLEFQLNGERRSLPLKNVRIDPGRKDVILKHFKKGMDVEV
                     IDEILP"
     gene            5487..>6085
                     /locus_tag="PKJ17_01395"
     CDS             5487..>6085
                     /locus_tag="PKJ17_01395"
                     /inference="COORDINATES: protein motif:HMM:NF015228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOD74650.1"
                     /translation="MRYYLDFEKKLEPLERRVFEIERFYDENEPHYAKELATLRKKIA
                     KIEKETYSELSNWQRSQLSRHLNRPHTLDYVHSLFTDFVELHGDRKFKDDPALVAGFA
                     RFEGVNVAVLGHQKGGDVREMAHRNFGMAHPEGYRKAMRVMDMAARWGKPIITFIDTP
                     GAFPGVGAEERGQAEAIASSIYFMFSLGVPTISIVIGEGG"
BASE COUNT         1406 a         1916 c         1734 g         1029 t
ORIGIN      
        1 ggtacccctc attgcggacg ggggcatcaa gttctccggc gacatcacaa aggccctggc
       61 agccggcgct gacaccgcca tgatagggaa cctcttcgcc ggtaccgacg agacaccggg
      121 ggaaacggtc ctctatcagg gcaggacata caaggtctac cgcggcatgg gctccctcga
      181 ggccatgcgc gaggggaaaa cacgcgaccg ctactcgatc cccgaggacg cgccggaatc
      241 gaagatcgtc cccgaaggca tagagggccg ggtgccatac cgtggttccc tctcgatctc
      301 cgtcaaccag ctcgtgggag gcctcaaggc cggcatgggg tacgtcggca ccagaacgat
      361 agaagagctc cagaaaaaac gcaggttcat caggataacg tcggcagccc tgcgcgagag
      421 tcatgtccac gacgtcatca tcaccaagga agcgccaaac tacaggatcg aataggatat
      481 ggacagccac caccgcgatt accatgacga actcatcctg atcctcgatt tcggctccca
      541 gtatacacag ctcatagccc gcaaggtccg cgaactcggc gtctattgcg agatctaccc
      601 ctacaacctg gaccttgacg ccataaggtc catgaagcct cgcggtatca tcctgtccgg
      661 cgggccggcc agcatcaatg agaagaacgc gcccatctgc gacagggcgg tcttcgagct
      721 gggcgttccc gtcctcggcg tctgctacgg ccttcagctt ctggccacgt ccttcggcgg
      781 caaggtggaa aaggccccga aacgtgaatt cggcaaggcc catctttata tcgagaaggc
      841 ggaccggttc cttgacggcg tgaaggacgg cgacatcgtc tggatgagcc acagcgacaa
      901 ggtgctcacc ctcccccgtg gttttgtcgc cctcgcccgg tccgacaact cctcctacgc
      961 ggcggtgcgc aacatgaacg gcaccgttta cggcgtccag ttccaccccg aggtccacca
     1021 caccccgaag ggcaagcgca tcctcaagaa cttcctctac aagatatgca gacttaaagg
     1081 tctcttctcg ccgcagtcct ttgtcgaact cgcgacggaa aagatacgca gggaggcggg
     1141 aagcgagaag gtcatctgcg ccttgagcgg cggcgtcgat tcctcggtgg tcgcagccct
     1201 cattcaccgc gccatcggcg acaacctgcg ctgcgtcttc gtcaataacg gcgtcctcag
     1261 gaagaacgag gctgaagagg tcatcgcggc ctacagggac atcctccacc tcaacctcaa
     1321 gtacgtcgat gccggtgagg cgttcctcaa ggccttaaag ggggtgaagg acccggaacg
     1381 gaaacggaag atcatcggga gactcttcat caggatattc gaggaagagg cccgcgacgg
     1441 cggctccgtc cggtacctgg cacagggaac cctctatccc gacgtcatcg agagcgtctc
     1501 cttcaagggg ccctcggcca cgatcaagag ccaccacaat gtcggcggcc tgccgaaacg
     1561 gatgaagatg accctcatcg agccgctgag ggagcttttc aaggacgagg tcagggtggt
     1621 gggccgagag cttggcgtcc ccgagtatat cgtcggcaga cagcccttcc ccggcccggg
     1681 tctcgccgtg aggataatcg gcgaggtgac ggaagaaagg gtccgggtac tccaggaagc
     1741 ggacagcatc atccgccagg agatcgagca caatccctct ttcaaacaca tatggcagtc
     1801 cttcggtata ctgatacccg tgaagaccgt cggcgtcatg ggcgacgaga ggacctacgc
     1861 caacgtcatc gccgtgcgtg tcgtcgaaag tgaggacgcc atgacggcgg actgggcacg
     1921 ccttccctac aagaccctcg atacggtggc gcgaagaata atcaacgagg tcgcggacgt
     1981 caacagggtg gtctacgata tctcctcgaa gccgccgagc acgatcgaat gggagtaagg
     2041 ggacatgccc gacttcgtcc acctccacct tcacacccag tacagtctcc ttgacggcgc
     2101 catccggttc gaccgcctct tcaacgcggc ccgggacttt ggcatgccgg cctgcgccat
     2161 cacggaccac ggcaacatgt tcggggcggc ggaattctac cttgaagcca accagcgcgg
     2221 actcaaaccc atcataggct gcgaggcgta tatagccccg gggtcccggt tcgaccagaa
     2281 aagggtcaag ggcgaggatg tggcctacca cgtcgtcctc ctcgcgatgg acaacagggg
     2341 gtatcagaac ctcctcaagc tcatcagcct cgcccacctg gaaggtttct actaccaccc
     2401 ccgcatcgac agggagatac tgagccagta ccacgaaggt ctcatctgcc tcacggcgtg
     2461 catcaaggga gagataccga acgccatcct ccggggcacc gacgagatgg tccgcaacca
     2521 cgtggattac tacctctcac tcttcggcga ccggctcttc ttcgagctcc aggacaacgg
     2581 tctcgaggag caaaggatag tcaacgaacg cctcatcgag ctctcgtccc attacggggt
     2641 gcccatcgtc gcgacgaacg actgccacta cctgcgccgc gaggaggcaa aggcccacga
     2701 gcttctgctg tgcatccaga cgggaaagac gatcaacgac aaggaccgcc tgagcttctc
     2761 gacggaccag ttctacttca agtcccccga cgagatagct ctcgccttct cccgctatcc
     2821 cgaggcgctt gccaacacga tgcgcgtcgc cgaaatgtgc aacgtcacca tcgacacgaa
     2881 cacgtaccac ttcccggaat tccacccccc cgccggcatg gacctcgacg agtacttcga
     2941 aaagctcgcg cggcagggtt tcgagaaacg gatgcccgag atacgggcgg cctacgattc
     3001 cttcggcgaa gagctcctcg accagtatcg caagaggttc gactacgagg cgggcgtcat
     3061 caggaaaacg gggttctccg ggtatttcct catcgtcgcc gacttcatca actacgcgaa
     3121 gacccacggc gttcccgtgg ggccggggcg cggttccgcg gcgggcagtc tcatcgcctt
     3181 ctgcctcggc atcaccgaca tcgaccccat caagtatgac ctcatcttcg agcgcttcct
     3241 gaacccggag cgcatcagca tgcccgacat cgacgtcgac ttctgccgca agggacgcga
     3301 cagggtcatc gaatacgtca cgaacaagta cggcaaggac aacgtggccc agatcaccac
     3361 cttcgggacc atgaagtcca aggcggcggt gcgtgatgtg gggcgggccc tcggcatgcc
     3421 ctacgcggag gtcgacaaga tagcaaagct catcaccacc gcggaccggg gcatcgaacg
     3481 ggcgatcaat gatgaacccg aggtgcggga actataccag agggacgacc gcgtgcggga
     3541 actcctcgac aacgcctgcg tcgtcgaagg gctggctcgc cacgcttcca cgcacgccgc
     3601 gggcatcgtg atcgccaaca agcatctctc cgagtacctt ccgctgtaca ggggcaaaca
     3661 cgatgaaacg gtgacgcaat acgacatgaa gatgatcgag aagatcggtc tcatcaagat
     3721 agacttcctc ggcctcgaga ccctcaccct catggacacg atcgtgaagc tcctcaaggc
     3781 cgaaggcatc gagatcgacc tcgcccggtt gccccttgac gacacaaaga cctacgagct
     3841 cctctcgtcg ggcaatacct ccggagtgtt ccagcttgaa agccgcggga tgcgcgacct
     3901 tctcacgaaa ctgaggccct cacagttcga cgacatcatg ccgctcatcg ccctgtacag
     3961 gcccggtccc ctgaaaagcg gcatggtcga cgagttcatc aagcgccgca acaacccgtc
     4021 gctcgtggcc tacgagacgc cgaagctcaa agacatcctc cacgacacgt acggcgtcat
     4081 catctaccag gaacagatca tgaagatcgc ctccgtgctc gccggcttct ccatgaagga
     4141 tgccgacgct ctgcgcaagg ccatgagcaa gaagatcccc gagcaactcg agaagtacaa
     4201 gcagcagttc gtcgacgggg ccgtggcgaa cgacgtcccg aaggacgtgg cggagaagat
     4261 ctacgacgtc atccagcgtt tcggggaata tggcttcaac aagtcccaca gcacggccta
     4321 cgggtacata gcctaccaga cggcatacct caaagcccat taccccatcc actatttcgc
     4381 ggccatgctc accaccgagg tgggcgacac ggacaaactc gtcaaatacg tcggggaatg
     4441 ccgggagtcg ggcatagaga tcctgccacc cgatatcaac atgagccaga gcgatttcac
     4501 catcgtcgat tcgaagatcc gctacgggct ctccggcgtc aggaacgtgg gcgaagcggc
     4561 gatcgaaagc atcatccagg cccgcgagga agcgggaggg ttcaagtcct tcgtccactt
     4621 ctgcagcgtc atcgattccc gcaaggtcaa caagaaggtc atcgaaagtc ttgccatggc
     4681 aggctgcttt gacagcctcg gcctcaacag gtctcaggtc ctctaccttg cccaggagat
     4741 gctcgacaag ctgacgaagc gggacacgaa gaacaatcac aagcagggca gcatctttgg
     4801 agaggctgag gtcgccgaga cgacggtctc cttcgacatc cccgccatgg atgaactctc
     4861 ccacgaagag atcctccttg gcgagaagga atcccttggt ttctacttca gcaagcatcc
     4921 cctgaaaccc tacgagaagc tcatcgccga gctcaccccc tacgacagcc agagtctcaa
     4981 ggaggcggac accgagcagg acgtgagcat ggcgggcatc gtgagcgtcc tgaaagagat
     5041 aacgacacgg cggggcgaca ggatggccta tgtcaccctg gaggatacga agggcaccgt
     5101 cgagaccatc tgcttccccg atctctacgg caagaaccac tttgtcatcc agagcggaaa
     5161 gcccctcatg gtcacggggt cgctcgagag gtccgaggac ggcagtgccc ggataaaggc
     5221 gaaggccgtg gcccttctcg aagcactgac gggtgaactc ctgaaaaccg tcaggatacg
     5281 gatacactgc gaggtcatca ggaaggaaga cctgagggtg ttgaaggaca tcctcttcag
     5341 cctccggggc aggtcgggcg ttcttctcga gttccagctc aacggggaaa gacggtccct
     5401 ccccctcaaa aacgtcagga tcgaccccgg acggaaagat gtcatcctca agcacttcaa
     5461 gaaaggcatg gacgtagagg tcatagatga gatactacct tgatttcgaa aagaagcttg
     5521 aaccgctgga aagacgggtc tttgagatag aacggttcta cgacgagaac gagccgcact
     5581 acgcgaagga gctcgcgacc ctcaggaaga agatcgcaaa gatagagaag gagacatatt
     5641 ccgaactgtc gaactggcag cgctcccagc tctcgcgaca cctcaaccgc ccgcacaccc
     5701 ttgattatgt ccacagtctc ttcaccgact tcgtggagct gcacggcgac aggaagttca
     5761 aggacgaccc ggcgctcgtt gccggttttg cccgcttcga aggagtcaac gtggccgtcc
     5821 tcggccatca gaagggcggt gacgtccgcg agatggccca caggaacttc ggcatggccc
     5881 atcccgaagg ctaccgcaag gcgatgcgcg tcatggacat ggccgcgaga tggggcaaac
     5941 ccatcataac cttcatcgac acgccggggg ctttccccgg tgtcggggcg gaagagcgcg
     6001 ggcaggcgga ggcgatcgcc tcgagtatct atttcatgtt ctccctcggc gtccccacca
     6061 tctccatcgt tatcggcgag ggggg
//
LOCUS       DAOPOA010000036         6066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_14257_length_6066_cov_3.204958, whole genome shotgun sequence.
ACCESSION   DAOPOA010000036 DAOPOA010000000
VERSION     DAOPOA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14257_length_6066_cov_3.204958"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..870
                     /locus_tag="PKJ17_01400"
     CDS             52..870
                     /locus_tag="PKJ17_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74651.1"
                     /translation="MFLFTVFALLLSGTILGCSLDALVSPDYGNKEYPTTELPDLTKK
                     PKQQEAAVEPNRRVTAPDEEAKLGPTPRSAAQTVRDETIDGRGTSVGTGANASQLKVR
                     TGSRTAFTGTVTAVDYVGKTISVKSAGKNLTFDLANPVVRGYKHTGEIQIGDVITVGY
                     IKNGIGIVKGENFHQDLADQTAPDELASSRKGKRSNKTAAKKSSNSHAAPVRVKYKVN
                     TLSFNDVDNNKDGKISPVELGSVLPSLTMQDFKKYDRNSDGDLSASEYRDVRKR"
     gene            complement(1027..1362)
                     /locus_tag="PKJ17_01405"
     CDS             complement(1027..1362)
                     /locus_tag="PKJ17_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOD74652.1"
                     /translation="MKVGYKVWIDNNGKAFGEGPYQVLKRIEETGSLHKAAMDLNMSY
                     RKAWLMLQAIEARLGFALLERKVGGVSGGGSNITPEGKKFLKNYEGFREDVRQTLEKT
                     FKKHFKTQV"
     gene            1648..3660
                     /locus_tag="PKJ17_01410"
     CDS             1648..3660
                     /locus_tag="PKJ17_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD74653.1"
                     /translation="MVSIPRSILFPVIITALAMVFTPPVGAQDQKVRDDAFTLGEIEV
                     KDRSEVSKNVTVEKIYEEDMRDFNKNNVAQAVNLLPGVTLTKNSRNEQMVYVRGFDMR
                     RVPIYLDGIPIYVPYDGYPDLGRFLTYDMSQITVSKGFTSVLYGPNTEGGAINMISRR
                     PQKTFEGNISTGYATGDTFTSYINLGTNQKKWYFQGGGSYVSSDYMRMSDNFRETPTE
                     NGGHRENSYYRDRKYNLKFGFTPFEGHEYALSYINQHGVKGNPPYAGVDPSVTVRYWQ
                     WPYWDKESVYFTSKTPIGQSSYFKIRLYYDQYQNSLYSYDDDSYSTMKKKYAWMSDFS
                     DNTGGGSIEVGTTLIPRNNIKAAFHYKEDKHKEHNQPNPYQHFKDEIWSAGLEDTLQI
                     TDRLYTIFGASYDNIRTIEAEDYNSTTKTFSNFARDSQSSFNPQIGFFYDLTKTARIH
                     ATVAKKTRLPTMKDKYSYKQGTALPNPNLRPEKSINYELGYQDTFFKKIGLKTAVFYS
                     DITDMVMQVKVPDPSKAGSTLNQNQNIGDVRRYGAELDVSGTIITNLDGGFNYTYIWS
                     DNRTDSTRITDIPKHKLAAYAKYMPLTSVSLIADVEYNSQRYSSSDGVQVARAFAVAN
                     FKAAWEFLKGLKIEGGVSNIFDRDYALSEGYPEAGRSFYSNLVYRF"
     gene            3860..4651
                     /locus_tag="PKJ17_01415"
     CDS             3860..4651
                     /locus_tag="PKJ17_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOD74654.1"
                     /translation="MKTNLLHNMRREFLVSLILHLCFFGAVAVFGHNLGRSREAVLVY
                     LTDELPGGGSGGPSGPATLSQDGGRPKAPPVRREPRGKQAARSLSTTKSPVKDLEKKP
                     QTRDEEVPGRPETAADLAGISSGQSWQAGGGEGGSGTGGGDGFGPGAGGGVGSGYGRG
                     VGSGRGPANARLQYLKEHFAYIRDLIMKHLAYPQMARKMGWKGKVIVAFVIRENGTVE
                     NSRVVRSSGYDVLDHNTLKTIREAQPFPRPPVKAELVIPIVYKLE"
     gene            4660..5256
                     /locus_tag="PKJ17_01420"
     CDS             4660..5256
                     /locus_tag="PKJ17_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOD74655.1"
                     /translation="MNTNHLDLQECLKKASLFHGDLCAGITLGTRMSILGLKAIGIDD
                     PMGKDRKNLIVFVETDRCATDAILAVTGCHPGKRTMKILDYGKMAATFVNLATGKAVR
                     VVAKDKTMDPNKEYDRETIEAEPHTEKYAMMPAEDLFELEEVDVDLWPEDMPGKPLRI
                     VVCSSCGERIMDMRDVRDNGRILCKPCAEGKTYWRKKT"
     gene            5272..>6066
                     /locus_tag="PKJ17_01425"
     CDS             5272..>6066
                     /locus_tag="PKJ17_01425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD74656.1"
                     /translation="MRDTAIDWNDVWKKESAKSSVKKRDVTFWNEKARNFSKKPWESN
                     YSEDFLKIMEPRRCWTVLDMACGTGALTIPLARYVRRITAVDFSPRMLEVLEEQCRVE
                     GIENVRTINASWEDDWSSRGIDEHDVVIASRCLVVDDLRKAVMKLNGAARERVYISTI
                     VGDGPHDRMIYEAVGRRLTPGPDYIYTYNLLHQLGIYANLFIIKDLRTESFRDSDEAL
                     AYYEHFLGKLTTEEREDLGSYLRKHLVPKNGRWVLDYRRITRWAVMW"
BASE COUNT         1528 a         1711 c         1680 g         1147 t
ORIGIN      
        1 cttgacatgc ccgggaaagg tcgttaaaac atgacatatt ttatgaagag aatgtttctg
       61 tttacagtct tcgccctctt gctttccggc accattctgg gctgctctct ggacgccctt
      121 gtctctccgg attacggcaa caaggaatac cccacgacgg aacttcccga cctgacgaag
      181 aagccgaagc aacaggaggc ggccgtcgag ccgaatcggc gcgtcaccgc ccccgacgaa
      241 gaagcaaagc tggggcctac gccaagatcg gcggcacaga ccgttcgaga cgaaacgatc
      301 gatggccggg ggacttccgt cggcaccggc gcgaacgctt cgcagctcaa ggtcagaacg
      361 ggctcgcgta cggccttcac gggtaccgtc accgccgtcg attatgtcgg gaagaccata
      421 tccgtgaaga gcgcgggcaa gaacctgaca ttcgaccttg cgaatcccgt cgtgcggggc
      481 tacaagcaca cgggagagat acagatcggg gacgttatca ccgtgggata tataaagaat
      541 ggcatcggca tcgtcaaggg agagaacttc caccaggacc ttgccgacca gaccgccccc
      601 gacgagctgg cctcatcccg gaagggtaaa cgatccaaca agacggcggc gaaaaaatct
      661 tccaacagcc acgccgctcc cgttcgcgtc aagtacaagg tgaacacgct ttccttcaac
      721 gacgtggaca acaacaagga cggcaagatc tcccctgttg aactgggcag cgtcctgcct
      781 tccctcacca tgcaggattt caagaaatac gaccgcaaca gtgatggcga cctgagcgca
      841 tcggaataca gggatgtcag gaagagataa cggcggcttg aatagcttga gtcgcttgaa
      901 tcgcttgagg gttaaaggcc cgggatagag cgatcctgta aggcttcggg atctgaccgc
      961 gctctatggt ctctcacttt caagcaattc aagcgactca agctattcaa gccgcctctg
     1021 tatggttcag acttgtgttt tgaagtgttt tttgaaggtt ttttccaggg tttgtctcac
     1081 gtcctcgcgg aagccttcgt agttcttcaa gaatttcttc ccttcagggg ttatgttgga
     1141 gccgccgccg gagacacccc ccaccttcct ttcgaggagc gcgaaaccca gcctcgcctc
     1201 tatcgcctga agcatcagcc atgccttgcg gtaggacatg ttcaggtcca tggccgcctt
     1261 gtggagggat cccgtctcct cgatccgctt gagcacctgg taggggccct cgccgaaggc
     1321 cttcccgttg ttatctatcc ataccttgta acccactttc atataaatgc ccccttgctc
     1381 gcgtaacaag gcaattatag ataacacctg taatcctgtc aagttacaaa atcacctccg
     1441 ccatatccct gcatttagcc ccagtgttca caatagacaa cagcggctct tgtattaact
     1501 ctcctttgat tttcaagaca tggaacaaaa caaacacagg atggacagaa tatcgaactg
     1561 ctgtgaaaat ttggtcttga cttgtcttta tatccggtga tatataacgt gcgttatatt
     1621 caaacagata taaggaggtc cgtccacatg gtttctatcc cccgatccat tctctttccg
     1681 gttatcatca cggcactggc catggtattc actccccccg tgggagccca agatcaaaaa
     1741 gtcagggacg atgcattcac gctcggagag atcgaggtga aggaccggtc cgaggtcagc
     1801 aagaatgtca cggtcgagaa gatctatgag gaggatatgc gcgacttcaa caagaacaac
     1861 gtcgcgcagg ccgtcaacct tctccccggc gtcaccttga cgaagaacag ccgaaacgaa
     1921 cagatggtgt acgtccgcgg attcgacatg aggcgcgtcc cgatctacct cgacggcatc
     1981 cccatctacg tcccctatga cggatatccc gacctcgggc gcttcctgac gtacgacatg
     2041 tcccagatca ccgtctcgaa ggggttcacg tccgtgcttt acggcccgaa caccgaagga
     2101 ggggcgatca acatgatatc aagaaggccg cagaagacct tcgaagggaa cataagcacc
     2161 gggtacgcca cgggagatac tttcacgagc tatatcaatc tcgggacaaa ccagaagaaa
     2221 tggtatttcc agggcggagg atcttatgtg agcagcgatt acatgaggat gtccgacaat
     2281 ttcagggaaa caccgacaga aaacggaggg caccgcgaga actcctacta cagggacagg
     2341 aaatacaacc tgaagttcgg gttcacgccc ttcgagggac atgaatatgc cttgagctac
     2401 ataaaccagc atggcgtgaa aggcaatcct ccgtacgcgg gcgtcgaccc gtccgttacg
     2461 gtccggtact ggcagtggcc ttactgggac aaggaaagtg tctacttcac gtcaaagact
     2521 cctatcgggc agagttccta tttcaagatc aggctctatt acgatcagta ccagaattcc
     2581 ctttacagtt acgacgatga cagctattcg acaatgaaga agaaatacgc ctggatgagc
     2641 gatttcagcg acaacaccgg cggtggctcc atcgaggtcg ggacgaccct catccccagg
     2701 aacaacataa aggccgcctt ccactacaaa gaggacaaac acaaggagca caaccagccg
     2761 aacccctacc agcatttcaa ggatgagata tggtccgccg ggctcgagga cacgcttcag
     2821 atcaccgaca ggctctacac catcttcggc gccagctatg acaacataag aacgatcgaa
     2881 gccgaagatt acaattccac aacgaagaca ttttccaatt tcgcgaggga cagccagtcc
     2941 tccttcaacc cgcagatagg gtttttctat gacctcacga agacggccag gatacacgcg
     3001 accgtggcga aaaagaccag gcttccaacg atgaaggaca aatactccta caaacaggga
     3061 acggccctgc ccaacccgaa cctgagaccc gagaaatcga tcaactacga actgggatat
     3121 caggacacct tcttcaagaa aatcggcctg aagaccgccg tcttctattc cgacataacg
     3181 gacatggtaa tgcaggtgaa ggttcccgat ccatccaagg ccgggtccac gttgaaccag
     3241 aaccagaata taggcgatgt gaggcgatac ggcgccgaac tcgatgtttc cggcaccatc
     3301 atcacgaacc tggatggcgg tttcaactac acctatatct ggtccgacaa caggacggac
     3361 tcgacgcgga taaccgacat cccgaagcac aaactcgccg cgtacgcgaa gtacatgcct
     3421 ctgacgagcg tttcgcttat cgcggacgtg gaatacaact cccagcgata cagttcctcc
     3481 gacggcgtcc aggtcgcgag ggctttcgca gtcgccaact tcaaggctgc ctgggagttc
     3541 ctgaaaggcc tgaagatcga aggcggcgta tcgaacatat tcgacaggga ttacgccctt
     3601 tccgagggtt atcccgaagc aggtagaagc ttctatagca atctggtgta caggttctaa
     3661 aggggaataa tggtcatcaa gaacagaacc gatcttctcg aaagactcca tggagacacc
     3721 ctgggcgcgt caccatcgag aggatcgtcg tcggtgcaca tttcaccggc gtcaatctgt
     3781 caaatggctg cggcgggatc tcatataccc ctgtggccga cctcatcctc aggaactgtc
     3841 aggaggtgtg gcgccgggca tgaagactaa ccttctccac aacatgcgac gggagttcct
     3901 ggtgtctctg atcctgcatc tgtgcttctt cggtgccgtg gcagttttcg gccataacct
     3961 gggcagatcc agggaggcgg tcctcgtcta cctcaccgac gaactccccg ggggaggctc
     4021 gggcggccca tcggggccgg ccacgctttc gcaggatggt ggaagaccga aagcgccgcc
     4081 cgtccgtcgc gagcctcgag ggaaacaggc ggcgcgatcg ttatcgacaa cgaagtctcc
     4141 cgtaaaggac ctcgaaaaga aaccacagac gagagacgag gaggtccctg gccgtccgga
     4201 gacagccgca gacctggccg gcatatccag cggccagtcg tggcaggctg gtggaggtga
     4261 aggcgggtct ggaaccggcg ggggggacgg tttcgggccc ggcgccggag ggggtgtggg
     4321 aagcggctac ggccggggtg tgggaagcgg caggggaccg gcaaacgcca ggctgcagta
     4381 tttaaaggag catttcgcgt acatccgcga tctcatcatg aaacatcttg cgtatcccca
     4441 gatggcacga aagatgggct ggaagggaaa ggtcatcgtc gccttcgtca tcagggagaa
     4501 cggaaccgtc gaaaattccc gcgtcgtccg aagctccggg tacgatgtcc tcgaccacaa
     4561 cacactgaag accatcaggg aggctcagcc ttttcccagg ccgcccgtaa aggcagaact
     4621 tgtgataccc atcgtctaca aactcgaata ggaggaggaa tgaacacaaa ccatctcgac
     4681 ctgcaggaat gcctcaaaaa ggcctcgctc ttccacggcg atctctgcgc ggggatcacg
     4741 ctgggtaccc gaatgtccat actggggctc aaggccatcg gcatcgacga ccccatggga
     4801 aaggaccgaa agaacctcat cgtcttcgtc gagactgacc gttgcgcgac cgacgcgatc
     4861 ctggccgtga cgggatgcca tcccggaaag aggacgatga agatactgga ctacggcaag
     4921 atggccgcga cctttgttaa cctcgcgacg ggaaaagccg ttcgcgtcgt agcgaaggac
     4981 aagacgatgg acccgaacaa ggaatacgac cgggagacga tcgaagcgga accccacacg
     5041 gagaaatacg ccatgatgcc cgccgaggac ctcttcgaac ttgaggaggt cgacgtggac
     5101 ctgtggcccg aggatatgcc cggcaagccc ctcaggatag tcgtgtgttc ctcctgcggg
     5161 gagaggatca tggacatgcg ggatgtacgc gacaacggca ggatcctctg caagccctgc
     5221 gcggaaggca aaacctactg gcggaagaag acgtgaagac aggatagtcc gatgagagat
     5281 acggcaattg actggaatga tgtctggaag aaggagagcg cgaaaagttc cgtgaaaaaa
     5341 cgggatgtca ccttctggaa cgagaaggcc cgcaacttct ccaagaagcc ctgggaatcg
     5401 aactactccg aggatttcct caagataatg gaacccaggc gctgttggac cgtgctggac
     5461 atggcctgcg ggacaggcgc gctgaccatc cccctggcac ggtacgtcag gaggataacc
     5521 gccgtcgact tctcaccccg gatgctcgaa gtcctcgagg agcaatgccg ggtggagggc
     5581 atcgagaacg tcaggaccat caacgcaagc tgggaggacg actggagctc ccggggcatc
     5641 gatgaacacg acgtggtgat cgcgtcgcgc tgtctcgtcg tcgacgacct ccggaaagcc
     5701 gtcatgaaat tgaacggtgc ggcccgggaa cgcgtctaca tttccaccat tgtcggggac
     5761 gggcctcatg accggatgat ctatgaggct gtcgggaggc gactcactcc cgggccggac
     5821 tacatataca cttacaacct cctgcaccag ctcggcatct acgccaacct gttcatcatc
     5881 aaggacctgc ggacggaatc ctttcgtgac agcgacgagg ccctcgcgta ctacgagcac
     5941 ttcctcggaa aactgacaac ggaggagagg gaggaccttg gcagttatct gcgaaagcac
     6001 ctcgtgccga agaacggcag gtgggtcctc gattacagga gaataacccg atgggcggtg
     6061 atgtgg
//
LOCUS       DAOPOA010000037         6043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_14334_length_6043_cov_3.631597, whole genome shotgun sequence.
ACCESSION   DAOPOA010000037 DAOPOA010000000
VERSION     DAOPOA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14334_length_6043_cov_3.631597"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PKJ17_01430"
     CDS             <1..993
                     /locus_tag="PKJ17_01430"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOD74657.1"
                     /translation="EGAPWGGMGRPDMILGNGQLLCDPRFKWPQATQHYQRDVPVFVT
                     NMYYPPWDPNVDHHDQEAIYVKYATEELRETVKFCEKVTGKKMDWDRLAATVELSDKT
                     WDLFIDTYELRKHIPTPMDTGDAMNTMVPLTFNLGTQEAYDFYVDLKAELEQKIADGV
                     GVADPEKYRVIWAAGLPSWFALGDFQYFNSKGVVFPVEVTYRGCEKVERLDLPKTTDP
                     LEHIAWRWVRYWTHWYDAARKRPGSTPKVERIIEYIEDYKCDGAVFHSAFSCRSWHAG
                     IMQQAATLKNVYKDIPILIMEGDIVDISSYNEADTHNRIDAFIETLAAYKKRQG"
     gene            1035..1874
                     /locus_tag="PKJ17_01435"
     CDS             1035..1874
                     /locus_tag="PKJ17_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOD74658.1"
                     /translation="MDYFAGVDIGSTMTKVVIVSDRIEASLIGPTGPEHRKLANRVME
                     EALRQARLTFEDLTYIVATGYGRINVPFADRQITEITCHAKGLHSLLPSVKTVVDIGG
                     QDSKGIKIENGKVMNFVMNDKCAAGTGRFLEIIADSLGVPLEKIGELSLTAQKIAPVS
                     STCTVFAEQEIISRLAGGEPIANLMAGIHDAVATRVYSLVKKLNVEPDIAVTGGGAKN
                     IGLVRALESKFGQKLLVPPEPLITGALGAALMGEDIFEKYAAEGQKPTRSGHKLEEAT
                     FFK"
     gene            1885..2658
                     /locus_tag="PKJ17_01440"
     CDS             1885..2658
                     /locus_tag="PKJ17_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOD74659.1"
                     /translation="MAYVLGIDVGSGYSKAVVLEGDEVRSWAVIPSGGDYREAGRAVA
                     QTALEKVNLTLSDVAWIVAAGYGGSMVDIAGETSTDLSCHAIGVHRLFPSVRTVIDVG
                     AQYSRAIRLHDEGKIANFIMNEKCAGGSGKFLQVAARILHMNVSDIGELSLKAQKPVE
                     FTTGCAVFAESEAVSRISEGASPADILAGIHNAMASKIVTLAMRVGLTPDYAVTGGGA
                     MDTGLVKAIAAELGTNVLIPQVPQITAALGAALIAKEKV"
     gene            2990..3556
                     /locus_tag="PKJ17_01445"
     CDS             2990..3556
                     /locus_tag="PKJ17_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74660.1"
                     /translation="MKKSTVLLGTALIVVCVAIALSQVPARAAGPLDACSLLTKAEAE
                     ALLNQASSSQKAGKVSAPAGNQCAYFFKKKGGTYSAKVKVSSSEEIKSEGIFKSARDV
                     FDRQKRARMANADTAQKMRSLGGLGDEAFWNGYDLWIVKGGYLLTILVQDQLAGSFKS
                     REAMDKARAEQDFNLSQRAASVILLKIK"
     gene            3580..4494
                     /locus_tag="PKJ17_01450"
     CDS             3580..4494
                     /locus_tag="PKJ17_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74661.1"
                     /translation="MTRKVLLGSLLLLFVVAVAAAEGPQGLKIVSVKPVKSGIYAIAS
                     VEFPKAWTDPGFTVEVNGKPVRMRQVGGGFSGDRNVADLMFMPGRGARQSVAVKCLVG
                     GRKTEAKTGFDWTATPFVAVPGHTGNREVITAKEKLTIAAVNVTGMRIFFNGKEVHAR
                     SSMRGDIETFTFEPAWKKGRNVLAINADKWDSGMIARNFTFFDLGEGGTLSPGQSALV
                     HLGREGTKSGPFYDVKVEGDSIAPPQGTTVRRDIIDEDGWLVSETWVAVELRARKEGT
                     AKVRVFVKPHFLRNMELEREFLITVGEG"
     gene            4600..4797
                     /locus_tag="PKJ17_01455"
     CDS             4600..4797
                     /locus_tag="PKJ17_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74662.1"
                     /translation="MAMIDEKKIRAYHSRSDNALVCPLCATDEERESAEPGDLVTEDV
                     IHDDNPMYCARCKKKIEKQGS"
     gene            complement(4818..>6043)
                     /locus_tag="PKJ17_01460"
     CDS             complement(4818..>6043)
                     /locus_tag="PKJ17_01460"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74663.1"
                     /translation="GNMVHPFIRRRQGKEPVVHLHPKLAPILDETLGVILFQEQILQV
                     AMAIAGFSAGEANMLRKAMGRKNARDELAKWHGRFVEGAEQQGIARGLATKIFDHISG
                     FADFGFCKSHAASFAILCYRSAYLKRYYPAEFYCALLNNQPMGFYAPEVITGDAKRHN
                     VPILPVDINRSMWNCSIEEGSVRIGFRYVKGIGKDKGDRIIKARREDIFTSLEDFADR
                     VRLDDRSVRNLVAVGAFSDIKRSRRQLLWEAGTLRAMGPAGFASLAREDRQENLPIPE
                     MSTAEETITDYAFQGFSASHHIMKLYRKALDSLGAVTSGVLPRCPPGSTVLTAGYMVC
                     LQMPPTAKGGFSFMTLEDEEGLINVVVRPEVYRMHRTTIRLEPFLLVEGVVEKKDGLI
                     NVRAKRFGNLRQRRG"
BASE COUNT         1354 a         1653 c         1851 g         1185 t
ORIGIN      
        1 gaaggcgctc cctggggcgg catgggcagg ccggacatga tcctcggcaa cggtcagctt
       61 ctgtgcgacc cgcggttcaa atggccgcag gcgacacagc actaccagag ggatgtccct
      121 gttttcgtta ccaacatgta ctatccgccg tgggacccga acgtagatca ccacgaccag
      181 gaagccattt acgtgaagta tgcgaccgag gagctccggg agaccgtcaa gttttgtgag
      241 aaggtcacgg gcaagaagat ggattgggac cgccttgccg cgacagtgga gctttccgac
      301 aagacgtggg atcttttcat cgacacctat gaactccgca aacacatacc aaccccgatg
      361 gacaccggcg acgccatgaa caccatggtt cccctcacgt tcaacctggg cacccaggag
      421 gcctacgact tttacgttga cctcaaagcg gagctggagc agaagatcgc cgacggcgtg
      481 ggtgttgccg accccgagaa gtacagggtc atctgggccg ccggtctgcc gagctggttc
      541 gccctcggag acttccagta tttcaacagc aaaggtgtcg tcttccccgt ggaagtgaca
      601 taccgcggtt gcgagaaggt cgagcgcctc gacctgccga aaacgactga cccgttggaa
      661 cacatcgcct ggagatgggt acgatactgg acgcactggt acgatgcggc aaggaagcgt
      721 ccgggttcga ccccgaaggt ggagcggatc atagagtata tcgaagacta caagtgcgac
      781 ggagcggtgt tccactccgc cttctcatgc agaagctggc acgcgggcat catgcagcag
      841 gccgccacgc tgaaaaacgt ttacaaggat atccccatcc tcatcatgga gggcgatatt
      901 gttgatataa gctcctacaa cgaggcagac acgcacaaca ggatcgatgc ctttatcgag
      961 actctcgcgg cctacaagaa gagacaaggc taaagtttag gggtctcgcg aaaatcagat
     1021 aggagcagta ccagatggat tattttgccg gtgtcgatat cggttccacc atgacaaagg
     1081 tggtgatcgt aagcgacagg atagaagcgt cgctcatagg gccgacaggc cctgagcacc
     1141 ggaaacttgc aaacagagtg atggaggagg cgctgcgtca agcgcgcctc accttcgaag
     1201 acctgacata catagttgcg actggatacg gcaggataaa cgttcccttt gcagaccgac
     1261 agatcactga gataacctgc cacgcgaaag gcttgcacag cctcctcccc tctgttaaaa
     1321 ccgtcgtcga tatcggcggt caggacagca aaggcatcaa gattgagaac ggcaaggtga
     1381 tgaactttgt catgaacgac aagtgcgccg ccggtacggg gcgctttctc gagatcatag
     1441 cggattctct cggcgtgccc ctcgaaaaga ttggcgagct atcactgacg gcgcagaaga
     1501 tcgcgcccgt gagcagcacg tgcaccgtgt tcgccgagca ggagatcatc tcccggctgg
     1561 cgggtggaga gcccatcgcg aacctcatgg cgggcattca cgacgcggtg gccacgaggg
     1621 tctattctct cgtcaagaaa ctgaacgtgg aacccgatat cgccgtgacg ggagggggag
     1681 cgaagaacat cggtctcgtc agggcactgg agtcaaagtt cgggcagaag cttctcgttc
     1741 cgccggaacc tctcataacg ggggctttag gagccgccct catgggcgag gatattttcg
     1801 aaaaatacgc cgcggagggg cagaagccaa cccgaagcgg acacaaactg gaagaggcga
     1861 cattcttcaa ataaggattc gaacgtggcg tatgtgcttg gcatagatgt aggttcaggt
     1921 tattcgaagg cggttgtcct cgaaggggac gaggtccgtt cgtgggccgt cataccgtcg
     1981 ggcggcgact accgtgaagc aggcagggcc gtggcccaga cggccctcga aaaagtgaac
     2041 ctcaccttga gcgatgtggc atggatcgtg gcagccggct atggcggcag catggtcgat
     2101 atagccgggg agacatccac ggacctatcc tgtcatgcca tcggagtgca ccgtcttttt
     2161 ccttcggtaa ggacggtcat cgacgtgggg gcacagtact cccgcgccat acgtctccat
     2221 gacgagggga agatagccaa cttcatcatg aacgaaaaat gcgccggcgg aagcggaaag
     2281 ttcctgcagg ttgccgcacg tattcttcac atgaacgtga gcgacattgg ggagctttcc
     2341 ctgaaggcgc aaaaaccggt cgaattcacc acgggatgcg cggtcttcgc cgaatcggag
     2401 gcggtgtcgc gcatatcgga aggtgccagt cccgccgata tcctggccgg gatccacaat
     2461 gccatggcgt cgaagatagt caccctggcc atgcgcgtgg ggctcacccc cgactacgcc
     2521 gttacgggcg gcggtgccat ggatacaggg ctcgtgaagg ccatagcggc cgaattggga
     2581 acaaacgtcc tcattcccca ggtgccccag ataacagcgg ccctcggcgc cgcactcata
     2641 gcgaaagaaa aagtttaagc cgcttgaact gcttgaaccg cttgagcggt caaaggcgaa
     2701 aaagacggct tcgcaatgcg gctccatgtc tcaaggacct gttgatgccc gacctctttt
     2761 cacggttttt tcctgaaacc tgaaacctga aacctgaaac ctgaaacctg aaaccctctt
     2821 ctgtttctgc aacccgcaac ccgcaaccgt ctcctgcccc tgaaacgaac acgtggtgtc
     2881 acccgcgtca ccctcctctt taagtgtgtg caatttgtag tggtatcggg tatattctta
     2941 ttgttcgaac aattccagcg gctgcaaaga atcttgcgag ggaggaatca tgaagaaaag
     3001 cactgtcctt ttgggtacgg ccctaattgt ggtatgtgtt gcgatcgcgt tgagtcaggt
     3061 cccggcgcgc gcggcgggtc ccctggatgc ctgcagtctg cttaccaagg ctgaggcgga
     3121 ggcgcttctc aatcaggcca gttcatcgca gaaggccggc aaggtgtccg ccccggcagg
     3181 gaaccagtgc gcctatttct tcaagaagaa aggcggcacc tacagcgcga aggtgaaggt
     3241 ctcctcctcc gaagagatca agagcgaagg gatcttcaaa tccgcgcggg acgtcttcga
     3301 cagacagaag agggcccgta tggccaatgc cgatacggcg caaaagatga ggagcctggg
     3361 cgggctgggc gatgaggcct tctggaatgg ctatgacctc tggatcgtca agggtgggta
     3421 ccttctcacc atactcgtcc aggaccagct ggcgggcagt ttcaagagca gggaagccat
     3481 ggataaggcc cgggcggagc aggacttcaa cctttcccag agggcggcgt ccgtaatact
     3541 cctgaagatc aaataagcgg cggtgaaagg gtgacggtca tgaccagaaa ggttcttctc
     3601 gggtccctgt tgttgctgtt cgttgttgcc gtcgccgcgg cggaagggcc gcaaggcctt
     3661 aagatcgtct ccgtcaagcc cgtgaagagc ggaatatacg cgatcgcatc ggtggaattc
     3721 ccgaaggcct ggaccgaccc cgggttcacc gtcgaagtga acggaaaacc tgtcaggatg
     3781 aggcaggtgg ggggcggttt ctccggggac cgcaacgtgg cggacctgat gttcatgccg
     3841 ggaaggggag cgagacaatc cgttgcggtg aagtgtttgg tgggaggaag aaagaccgag
     3901 gccaagaccg ggttcgattg gacggccacg ccttttgtcg ccgtgccggg gcacacgggg
     3961 aaccgggaag tgatcacggc gaaggagaaa ctgaccatcg ccgcggtcaa tgtcacgggg
     4021 atgaggatat tcttcaacgg caaggaggtc cacgcgagat cttcgatgag aggcgatatc
     4081 gagaccttca ccttcgaacc cgcctggaaa aagggaagga acgtgcttgc cataaacgct
     4141 gacaaatggg actcaggcat gatcgcgagg aacttcacct tttttgacct cggggagggc
     4201 gggactctat ccccagggca gagcgccctc gtgcaccttg ggcgggaagg cacgaagagc
     4261 ggccccttct atgatgtgaa ggtcgaaggc gattccattg ctcctcctca gggcaccacg
     4321 gtccgccgcg acatcattga cgaggacgga tggcttgttt ccgagacatg ggttgcggtg
     4381 gagttgaggg cccggaagga gggaaccgcg aaggtgcgtg ttttcgtcaa gccccacttt
     4441 cttcggaaca tggaactgga aagagagttt ttgatcaccg tcggggaagg gtgaaaagaa
     4501 ggcgttctgc ccttgtgcgt ggttgacacg cgacagtcgc attactatag tacgccagtg
     4561 ataaccggtg aacgaagcga caaaagaagg ggaggaacca tggcgatgat cgatgaaaag
     4621 aagattaggg cctatcactc caggagcgat aacgcccttg tttgtccgtt gtgcgcgacg
     4681 gatgaggagc gagagtccgc ggagccggga gatcttgtca cggaggacgt gatccatgac
     4741 gacaatccca tgtactgcgc gagatgcaag aagaagatcg agaaacaggg ctcctgacgc
     4801 gggggcggga tcggggccta tcccctcctt tgcctcaggt tcccgaatct ctttgcccgg
     4861 acgttgataa gcccgtcctt cttctcgacg acaccctcca cgagcagaaa aggctcgagg
     4921 cgtatggtgg tccggtgcat cctgtagacc tccggccgta ccacgacgtt gatgagtccc
     4981 tcctcgtcct cgagggtcat gaatgagaac cctcccttcg cggtgggggg catctggaga
     5041 cataccatgt acccggcggt gagcacggtc gaacccggcg ggcaacgggg aagaactccg
     5101 ctcgtaaccg ctcccaggct gtcgagggcc tttcgataga gtttcattat atggtgcgaa
     5161 gcggaaaagc cctggaaggc ataatcggtt atcgtctctt ctgctgtgct catctccggt
     5221 atggggaggt tttcctgccg gtcttcgcgc gcgagcgaag cgaatcccgc tggtcccatt
     5281 gcgcgtagcg tccccgcctc ccacagaagc tgtctccttg accgtttgat gtcgctgaag
     5341 gccccgacgg cgacgaggtt gcgcactgac cgatcgtcga gtcggaccct gtcggcaaag
     5401 tcctcgagcg atgtgaagat gtcctcccgc cgtgccttga tgatacggtc gcccttgtct
     5461 ttgccgatgc ctttcacgta cctgaaaccg atcctcacgg acccctcctc gatggagcag
     5521 ttccacatgc tcctgttgat gtccacggga aggatgggga cgttgtgccg tttcgcgtcc
     5581 cccgttatca cctcgggggc gtaaaatccc atgggttgat tgttgagaag ggcgcagtag
     5641 aattcggctg gatagtatcg tttcaggtat gccgaacgat agcacaggat ggcaaagctg
     5701 gcggcgtggc tcttgcagaa accgaaatcg gcaaaaccgc ttatatgatc gaagatcttt
     5761 gtcgctagac cgcgcgctat cccctgttgt tccgccccct cgacgaaacg gccatgccac
     5821 ttcgcgagct catcgcgggc gttcttccgg cccatagcct tgcgcagcat gttggcctca
     5881 cccgcggaga atccggctat ggccattgcc acctggagta tctgttcctg gaagaggatc
     5941 accccgagcg tttcgtcaag gatgggggcc agcttcgggt ggagatggac gacgggttcc
     6001 ttcccctgcc ggcgccggat gaaggggtgc accatgttgc cct
//
LOCUS       DAOPOA010000038         5992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_14485_length_5992_cov_3.509180, whole genome shotgun sequence.
ACCESSION   DAOPOA010000038 DAOPOA010000000
VERSION     DAOPOA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14485_length_5992_cov_3.509180"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PKJ17_01465"
     CDS             complement(<1..648)
                     /locus_tag="PKJ17_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HOD74664.1"
                     /translation="MDQITTSMTTFMQDHPDLKYIFFGGKGGVGKTVMAGTAALWAAK
                     QGKKTLLASTNPVHSLSNLLGQDVFGKTAVVCDEALCHAFEIDTKDTIERSKQEIREK
                     INWFLKFADITTKADEFVESATMNPAFEESAMFENMTDIMFKDEYDFYVFDTAPTANA
                     RRLLGMSKVYSLWVDKMLKSREEAKSLREMLSFSKKEEQDPLMDNSSISRRGWPGR"
     gene            complement(664..1644)
                     /locus_tag="PKJ17_01470"
     CDS             complement(664..1644)
                     /locus_tag="PKJ17_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HOD74665.1"
                     /translation="MVPISLAKIFEQYPDRHYIMFGGKGGLGKTTFSAAAAYYLAKQG
                     KKVLVFSVDPQASLSDIFKKDIFGKGPTEIMPNLYAQEIDADHRVREYQQEIRQKILD
                     MYGMDKIPEEIESYIQAAAAEPAMEESAIFDEVVDIVVKGGYDYYIYDLVPLGHALYY
                     LSMASVYDAWIGKITGLRQQMREYDQVAATIRRDKDLDEDAILNELLYIKDRINRSSS
                     ILTDKQKTAFFFVLTPEEMIINDTLKAADLFAKFDVPLSGYIVNRVLPAELKGQNIPE
                     YLKHRLDMQDHYLKVIDEVFKDQIHAWVPEMERDVTGLEMIEKLSNTMFG"
     gene            complement(1718..2002)
                     /locus_tag="PKJ17_01475"
     CDS             complement(1718..2002)
                     /locus_tag="PKJ17_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74666.1"
                     /translation="MGEKEGKEKKKKGGFFGAVKEFVYGVAAHDSARFALKSRASMEH
                     LFILITMGDMLGVPILPPYYSLRLLPYVVPQISTWKRRMLREKDLTDALA"
     gene            complement(2054..3745)
                     /locus_tag="PKJ17_01480"
     CDS             complement(2054..3745)
                     /locus_tag="PKJ17_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOD74667.1"
                     /translation="MNSVVVLIISLAVFFVGYRLYAGYIDRKVIKTDPKRATPAKMYM
                     DGVEFMPTSKNVLFGYQFKSIAGAGPIIGPIIAIQWGWLPALVWILLGTFFIGWVQDY
                     GSAIMAMRHEGASFGGLSHKLISPRARTILLSFVYFYLLLIAGAFGNVVVDTAIRLKP
                     APMAWLFLTVAGIVAGQMIYRWKRDIILTTVVTVVIAMVGIWVGTVAPSNQIIGESLS
                     NSRLLWALAAFVFCYFAAVMPIWKFALPVNYVAAYIVFIGLFVGIIGIFVLHPDFTLP
                     AVTSFSIPIGPIWPIMFVTIACGAISGWHSIVSSSGTARQLENELDARPVGAGVMFVE
                     MMLAAFALIIAGTIYASPADYGAAIKLGPGGVFAAGVAKFLNAVGLPTAIGRSYGSVM
                     MVVLAITIMQLVVRFMRVATSELLAGWHPVFRNAHVGTFIASVLGMVLVLTGWWQYLW
                     VLFGGANQLMASLALMLITAWLMSEGKSYSWALWPMIFMFITTIAALLYTSYNLLNKV
                     LTGAVKGQALIGNTLMGLVGIFLVVAAIILGIEGVKAFAKYRALKNNKMPESAQA"
     gene            3911..4846
                     /locus_tag="PKJ17_01485"
     CDS             3911..4846
                     /locus_tag="PKJ17_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOD74668.1"
                     /translation="MKVCFFGVGGVGGYYGALLTRYFDSTGTGRTYFIARGRHGAAIV
                     ERGLTLKKDGGGAEIRVTPFACSNTVAGLPVCDMIVVSVKGYDLDAATEQVAAITDDR
                     TIVLPLLNGADIYERMRRRLDKGCIFASCLYLGTHIESPGVIFQMGGSGQIAIGRDPL
                     YPDIYPEKLIELFRNAGILIEFHEDVNVEIWKKYIFIAPFALVTAAWSKTIGEVAADA
                     RLARLVRDIMKEIAAIAKALGIELPPDIVETSFLKAGQFPFETKTSFQRDVETKGRQS
                     EWDLFGGTVIRYADKLGVPAENTRATLEQLLKGLK"
     gene            complement(5085..5765)
                     /locus_tag="PKJ17_01490"
     CDS             complement(5085..5765)
                     /locus_tag="PKJ17_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOD74669.1"
                     /translation="MNITKEELKDVLLRHKQWLSLDEGGECADLSGIDLSEANLAGAN
                     LAGADLSRANLTRADLSQANLFDARLTGADLSGANLSRANLSRAYMARSDLARADLSE
                     ANLSDVNLVRANVSEADLVGATLTGINLSNTDLSNTDLSGATLADAILARADLTAAIL
                     ARADLSRAYLARADLFEADLTGADLSEANLTGANLCEADLTGADLSRAKLDNAEFAGA
                     QWIDGTKK"
BASE COUNT         1210 a         1694 c         1798 g         1290 t
ORIGIN      
        1 gcgcccgggc catcctctcc ttgaaattga ggagttatcc atgagggggt cctgctcttc
       61 ctttttggag aaggagagca tctccctcaa ggattttgcc tcctccctgc tcttgagcat
      121 cttgtccacc cagagcgagt agaccttcga catgccgagc aggcggcggg cgttcgccgt
      181 cggggccgtg tcgaagacgt agaagtcgta ttcgtccttg aacatgatgt ccgtcatgtt
      241 ctcgaacatc gccgattcct cgaaggcggg gttcatcgtg gccgattcga cgaactcatc
      301 ggccttggtg gtgatgtcgg cgaacttaag gaaccagttt atcttctccc tgatctcctg
      361 cttggacctc tctatggtgt ccttcgtgtc tatctcgaag gcgtgacaga gggcctcgtc
      421 gcagacgacg gccgtcttgc cgaagacgtc ctgcccgagg aggttggaga ggctgtggac
      481 ggggttggtg gacgccagca gggtcttctt tccctgtttc gctgcccaga gcgccgccgt
      541 gccggccatc accgtcttgc cgactccgcc tttcccgccg aagaagatgt atttcagatc
      601 gggatggtcc tgcatgaacg tggtcatact tgtcgttatt tggtccattg aagactccct
      661 cccttaacca aacatggtat tcgacagctt ctcgatcatc tcgagaccgg tgacatcccg
      721 ttccatctcg gggacccagg cgtgtatctg gtccttgaag acctcgtcga tcaccttcag
      781 gtaatggtcc tgcatgtcga gacggtgttt gagatactcg gggatgttct gccctttcaa
      841 ctcggcgggg aggaccctgt tgacgatgta gccgcttaag ggtacgtcga acttcgcgaa
      901 gaggtcggcg gccttcaggg tgtcgttgat gatcatctct tcgggggtga ggacgaagaa
      961 gaatgccgtc ttttgcttgt ccgtgaggat gctcgacgac ctgttgatgc ggtccttgat
     1021 gtagagaagt tcattgagaa tggcgtcctc gtcgaggtcc ttgtccctgc gtatcgtggc
     1081 ggccacctga tcgtattcgc gcatttgctg gcgaagcccg gtgatcttcc ctatccaggc
     1141 gtcatagacg gaggccatgc tgaggtaata caaggcatgt ccgagaggca cgagatcgta
     1201 aatgtagtag tcgtaaccgc ctttcacgac gatgtcgacg acctcgtcga agatggcgct
     1261 ctcctccatg gccggctccg ccgccgcggc ctgaatgtag ctctcgatct cctcgggtat
     1321 cttgtccatg ccgtacatgt ccaggatctt ctggcggatc tcctgctggt attccctgac
     1381 acggtggtcc gcgtcgatct cctgcgcgta caggttgggc atgatctccg tcgggccctt
     1441 gccgaagata tccttcttga agatatcgct caaggatgcc tggggatcga cggaaaagac
     1501 gagcaccttc ttgccctgtt tcgccagata ataggcggct gccgcggaaa acgtggtctt
     1561 gcccagacct cccttgccgc cgaacatgat gtagtgccgg tccggatact gttcgaatat
     1621 ctttgcgagt gaaatgggaa ccaccccctt gttttgaatt aggttgaact tctcaagcct
     1681 ctatccggca aagatgaatt gccataccgc cgcggcctca tgccagggcg tccgtcaggt
     1741 ccttttcccg gagcatcctg cgcttccagg tggatatctg ggggacgacg tagggcagaa
     1801 gcctcagcga atagtaaggg ggcaggatag gaaccccgag catgtccccc atggtgatga
     1861 ggatgaacag gtgctccatg cttgcccgag atttcagggc gaaacgcgcc gaatcgtgcg
     1921 ccgcgacgcc gtagacgaac tcctttaccg ccccgaaaaa accacctttt ttcttcttct
     1981 cttttccttc tttttcaccc atgttccttg tgtctcctta ataatgatgg ggttgcgctc
     2041 cttctttcgt atctcatgcc tgggccgatt ccggcatctt gttgttcttc agggccctgt
     2101 attttgcgaa cgccttcact ccctcaatgc cgaggataat cgccgcgacg acgaggaata
     2161 tgcccacgag ccccatgagg gtattgccta tgagggcctg acccttcacg gctcccgtga
     2221 gtaccttgtt gagaaggttg taggacgtgt agaggagggc cgcgatggtc gtgatgaaca
     2281 tgaatatcat cggccacaat gcccatgaat acgactttcc ctccgacatg agccacgcag
     2341 tgatgagcat gagcgccagc gacgccatga gctggttggc gcctccgaag agcacccaca
     2401 ggtactgcca ccacccggtg agaacaagga ccatgccgag gacactggcg atgaaggtgc
     2461 ccacgtgggc gttgcggaaa acaggatgcc agccggccag aagctccgac gtggcgaccc
     2521 tcatgaagcg cacgacgagc tgcatgatcg tgatggccag caccaccatc atgacgctcc
     2581 cgtaagacct gccgatagcc gtcgggaggc ccacggcgtt gaggaatttc gcgacgccgg
     2641 ccgcgaagac ccctccggga ccgagcttga tggccgcccc gtaatcggcg ggggaggcgt
     2701 atatggtgcc ggctatgatg agggcgaagg ctgccagcat catctccacg aacatgacgc
     2761 ctgcgccgac gggccgcgcg tcgagttcgt tctcaagctg ccgcgcggtg cccgaactcg
     2821 acacgatgct gtgccagccc gagatggccc cgcaggctat ggtaacgaac atgatgggcc
     2881 agatggggcc gatggggatc gagaagctcg taacggcggg cagggtgaag tcggggtgca
     2941 ggacgaagat cccgatgatc ccgacgaaaa ggccgatgaa gacgatgtag gccgccacgt
     3001 agttgacggg gagcgcgaac ttccatatgg gcatcaccgc cgcgaaatag cagaaaacga
     3061 aggccgcgag cgcccagagg aggcggctgt tcgaaaggga ctcgcctatt atctggttcg
     3121 aaggcgccac ggtgccgacc cagatgccga ccatggcgat gacgaccgtg acgaccgtgg
     3181 tgaggatgat gtcccgtttc cacctgtaga tcatctgtcc cgcgacgatg ccggcaacgg
     3241 tcaggaagag ccatgccatg ggcgccggtt tcagccgtat cgccgtgtcg acgacgacgt
     3301 tgccgaaggc gccggcgatg aggaggaggt agaaatagac gaaggagagg aggatggtcc
     3361 gcgcgcgcgg gctgatgagc ttgtggctca agcctccgaa agaggccccc tcgtgacgca
     3421 tggccattat cgcgctcccg tagtcctgga cccagccgat gaagaaggtt cccagcagta
     3481 tccagacgag cgccgggagc cagccccact ggatggcgat gatggggccg atgatcggtc
     3541 ccgcgccggc gatcgatttg aactggtagc cgaagaggac gttcttgctc gtgggcatga
     3601 attccacccc gtccatgtac atcttggccg gtgtggccct ttttggatcc gtcttgatga
     3661 ccttcctgtc gatgtacccg gcatacaggc ggtagccgac aaagaacacg gccaaactga
     3721 ttatcaggac aacgactgaa ttcatacatt cacctccgtg ttgcattgga atggtgttat
     3781 gcgttcacat gttgtcgctg cgtcattttt gtgatctgat ttaccttatt ctcctcccat
     3841 taaaaccatt ttgcaagaaa gaaattaagg tgttatgctg gttcatccct agttcggaaa
     3901 ggaatgtccc atgaaggtgt gtttctttgg cgtcggagga gtgggtgggt actacggagc
     3961 cctgctcacc cggtattttg actcgaccgg cacgggaaga acgtatttca tcgcacgggg
     4021 gcggcacggg gccgccatcg tcgagagggg gctcactttg aagaaggacg ggggaggggc
     4081 agagatacgg gtgacgccct ttgcctgttc caacaccgtc gccggattgc ccgtatgtga
     4141 catgatagtc gtgtccgtga agggctacga cctcgatgcc gccacggagc aggtcgccgc
     4201 gataacggac gacaggacca tcgtattgcc tctcctcaac ggtgccgata tctacgagcg
     4261 gatgcggcgg cggctggaca aaggatgcat cttcgcctcc tgcctctatc tcggtactca
     4321 tatagaatcg cccggcgtca tcttccagat gggtggaagc ggccagatag cgatcggcag
     4381 ggaccctctg tacccggaca tctatcccga aaagctgatc gagctcttca ggaacgccgg
     4441 catccttatc gaattccacg aggacgtgaa tgtggagata tggaagaagt acatcttcat
     4501 cgcccccttc gcgttggtga ccgccgcctg gtccaaaacg atcggtgagg tcgcggctga
     4561 cgccagactt gcccgtctcg tcagggacat catgaaggag atagccgcca tcgcgaaggc
     4621 cctcgggata gaactaccgc ccgacatcgt ggagacctcc ttcctgaagg cgggccagtt
     4681 ccccttcgag acgaaaacgt ccttccagag agacgtcgag acgaaaggca ggcaaagcga
     4741 atgggacctc ttcggaggca cggtcatccg ctatgccgac aagctcggcg tccccgcgga
     4801 gaacacgagg gcgaccctgg aacaactact gaagggttta aagtgacacg cgttatcggg
     4861 gcctccgtgg tcgttgcctg gcaggtcccc ggggttgcgc ttcccggaag ctgtattata
     4921 attaagtata aggtaagctt gcctcgccac tttggtccga agggttccgg cccccaagcc
     4981 ggagtgaagg ctccccagcc ttcacaaccc ttcgccaagg tggtttcttt agtgagtctt
     5041 ccggtacttt tggcagtccg aggccttaac cgatcggaag gattctattt ctttgttcca
     5101 tcgatccatt gagcacccgc gaactcggca ttatccagtt tggcacggga tagatccgcg
     5161 ccggtgaggt cggcctcgca gagattcgcg ccggtgagat tcgcctcact aagatccgcg
     5221 ccggtgaggt cggcctcgaa gagatccgcc cgggccaggt aagcacggga gaggtccgcg
     5281 cgagccagga tggcagccgt gaggtccgcg cgagccagga tggcatcggc gagggtggcg
     5341 ccggacaggt ctgtattgga caggtctgta ttggacaggt tgatgcctgt gagggtggcg
     5401 ccgaccagat cagcttcgga gacattggcc cggacgagat tgacgtcgga cagattcgcc
     5461 tcggacagat ccgcccgggc gagatccgaa cgggccatgt atgcccgcga gaggttggcc
     5521 cgcgagaggt tggcacccga aaggtcggcc ccggtcaggc gggcatcaaa gagattggcc
     5581 tgggacaggt ccgcgcgagt gaggttggca cgggatagat ccgccccggc aagattcgcc
     5641 ccggcaaggt tggcctcaga gaggtctatg ccggagagat cggcgcattc cccgccctcg
     5701 tccagcgaga gccactgctt gtgtctcagt aggacatctt tcagttcttc ttttgtgata
     5761 ttcataagac tccctcatgg atgtgtacga acagagatac tactaatcca cgaagcgttt
     5821 atcaaataaa ttcggctaga cgattgtttt cccgcacccc cattcgcccc gaaacacctc
     5881 ggagaaagcg agcattcttg aaatgctgag atgccacctg gcccgtcagg tcgtacgcta
     5941 acgaccaatt gcgagggata gcccggtctc ccctggccgg ttccgtgttc cc
//
LOCUS       DAOPOA010000039         5968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_14566_length_5968_cov_3.131067, whole genome shotgun sequence.
ACCESSION   DAOPOA010000039 DAOPOA010000000
VERSION     DAOPOA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14566_length_5968_cov_3.131067"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(536..2392)
                     /locus_tag="PKJ17_01495"
     CDS             complement(536..2392)
                     /locus_tag="PKJ17_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOD74670.1"
                     /translation="MQFTKRLGERIGYFIANGIFHKSILREAFPGWMHFFIFWGFLIL
                     TVGTALVAIQADFTDLLFKVQFLKGGFYLIFSFFLDLAGLMAIIGILMAMYRRYVTKP
                     TRLDNKPDDAIVLVWILVVLVTGFLAEGVRIAHSMPDYEKWSFVGYVTAYMFTGMPKE
                     SIEVTHKVFWYVHMVISFGLLGYIVHSRLLHIITSSLNMMFRGVEDVPEGARGAIAPI
                     EDFENAEEFGINSIEGFTWRQIFDLDACTRCGRCQDLCPAYNTDKPLSPKQFIQDLKA
                     EWERHCSGVKNEDGMIDTTILEETLWSCTACLACQVNCPVSIPTFDKNIEMRRYLTLT
                     LSKTTSETKLLFKNLQKNNNPYGMGKKERLGWTEGLDVRNAMEQEVEYLYWVGCVASL
                     DDRNRKVARSFATILNQAGVNFGILGTDEMCCGDPSRRCGNEEQYLGTAQGNVELLNE
                     IGIKKIITTCPHCYTTIKKEYAQLGGNFEVYHQAEFINKLINEGKLKLNPALEGKVTL
                     HDPCYLGRVNGIFDQPRNVLGKVSKGTYEEMGRNHNQSFCCGGGGGRIWMEEHHKRIN
                     HARIDEAIAIDANTVVTACPYCLIMMEDAIKDKEKIETMKALDLSEVVVKGL"
     gene            2778..3794
                     /locus_tag="PKJ17_01500"
     CDS             2778..3794
                     /locus_tag="PKJ17_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOD74671.1"
                     /translation="MRRRLTLCVLCISILFLTIPLVANASQVTHKVKRGDSLHNLAKK
                     YHVSVSHLKNVNGLRSNKLSLGQTIVIKNNEENPAVRETRKGPSSKAIRAHVRQEEPA
                     EPVISENDDEFVEYRVKRGDTLDKIASKFNVEKDDLIATNDLTSRKSRKLSPGKTILI
                     PRVMEDDGDDGDTIVSFKNAPVKPWKNSDEKYMLVKVAKSFMGAPYKYGGNTLRGLDC
                     SAYVKKIYEIFDVELPRSAREQYKVGNKISREELSVGDLVFFKTRRYAKYPTHVGIFI
                     GDGNFIHASSGKRRLGVKIDTLTSGYYSGAYIGATRIKGNVDAPTSRGPEATQPVEKP
                     SNNS"
     gene            3803..4987
                     /locus_tag="PKJ17_01505"
     CDS             3803..4987
                     /locus_tag="PKJ17_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74672.1"
                     /translation="MRKEHFLVGLSIALYLFGHLALVWNLEPAISFFYVTSWWSYIIL
                     LDSFVSWKTGEPLFLNRSLPAVIIVSCGYWCAFELLNLRIENWFYINVPHAIALRYAG
                     YLLAYGTVIPAIGLTAAIASPLLGRIRVRPVTTPRSYPARAISCGIALFLLTLIFPGY
                     LFGLAWVFATPLIDGVNYRAGYRSFMGDLERGQIGRLLGALASGLVCGLLWETWNSLS
                     PVKWVYTVPFFERMKIFEMPLPGYIGFPVFGVETVAFLDLFEGLRRKKSLAALTLCGA
                     LLTSVLSFSLIDRHTVFSRTTPVEQLLFLSRKSKDALLTKGVRTNLTVDTSLLGPGEA
                     QRMRLVNLRGLGYENYLKLEVHGITSVDGLARLDETRLAQMLGEKNLLRIHIYQSAAR
                     PH"
     gene            5032..5496
                     /locus_tag="PKJ17_01510"
     CDS             5032..5496
                     /locus_tag="PKJ17_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74673.1"
                     /translation="MARKKEKELIHKPDMLVTAFESVSLYIRDNRRQCVYGLVALLII
                     VAAVASYIVYANHQAQKVQYKLTQGITAWETYEQTRAAGEIDKAEDIFQKIASGASGK
                     PRYISKLYLANINMARGKRDEALKLYQDVSRNSSNKTLTYLADNAIKTLEKK"
     gene            complement(5643..>5968)
                     /locus_tag="PKJ17_01515"
     CDS             complement(5643..>5968)
                     /locus_tag="PKJ17_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOD74674.1"
                     /translation="GIFAIMLLSISERKMEIGIRRVVGSKKRDIIIQFLSESVIVALV
                     GGIGGILAGLLITVIVDIIGGFPIVFSMNIPLALAISMFIGVLAGIYPAMEGTKYEPV
                     KALYS"
BASE COUNT         1400 a         1687 c         1508 g         1373 t
ORIGIN      
        1 agaacgagca ctttgccgag aagcttcttg atgaaccagc ttatgatgaa ccccaggatc
       61 atcacgatga ccatgacgat gatactgaga aagacctcct gaaaattgtc gaaaagatcc
      121 aggaacatgt cctgaaaaaa cttcatgatt tcctgcatgg gaacatatta tgcaaagtgc
      181 tccgccatgt caatggaaat tggggcccta tttttttatt gacattcctc attgattcgt
      241 ttaggaaatt gtaaaaatcg aaaggaggga gtatacatgc ctgcaaaagt tgatcaagat
      301 gcctgcacag gatgtggagc ctgtgctgag atctgccccg ccgatgctat cactgtcgat
      361 gacacggcaa aagttgatcc tgaactgtgc accgaatgcg gcgcctgtac agaagaatgt
      421 cccgtggaag ctattacttt ggaagagtaa tcctgccaac ttgacgcgca aattataaaa
      481 ggatggggtg tgaccacccc atccttttat cgtatccttt ccttattctc ttctcttaca
      541 gaccctttac caccacctct gacaggtcca gggccttcat ggtctcgatc ttctctttgt
      601 ccttaatggc gtcttccatc atgataaggc agtacgggca cgccgttacg acggtgttgg
      661 catcgatcgc tattgcctcg tcgatcctcg catggttgat cctcttgtgg tgttcttcca
      721 tccatatccg gccgccgccg ccgccgcaac agaagctctg gttgtggttc ctgcccattt
      781 cctcgtacgt acccttgctg accttaccca gaacgttgcg gggctggtcg aagatgccgt
      841 tcaccctgcc gagataacac gggtcgtgga gcgtcacctt gccttcgagg gcgggattca
      901 gtttgagctt cccttcgttg atcagtttgt tgatgaattc cgcctggtga tagacctcaa
      961 agttgccgcc gagctgggcg tattccttct tgatggtggt gtagcagtgg ggacaggtgg
     1021 ttattatctt cttgatccct atctcgttga gaagctcaac attgccctga gcggtgccga
     1081 gatactgttc ttcgttgccg cagcgccgtg acggatcgcc acagcacatc tcgtcggtgc
     1141 cgaggatgcc gaaattgacg cctgcctgat tgagtatcgt ggcaaaggac ctcgccacct
     1201 ttctgttcct gtcgtcaagg gaggcaacac aacctaccca gtagaggtac tccacttcct
     1261 gctccatggc attcctgacg tcgaggccct ccgtccaccc cagtctctct ttcttgccca
     1321 tgccgtaggg gttgttgttc ttctggaggt tcttgaagag aagcttcgtt tccgacgtgg
     1381 tcttgctcag cgtaagcgtg agatagcgcc tcatctcgat gttcttgtcg aaggtgggga
     1441 tcgacaccgg gcagttcacc tggcaggcaa ggcaggcggt acaggaccac agggtctctt
     1501 ccaggatggt ggtatcaatc atgccgtctt cattcttcac acccgagcag tgacgttccc
     1561 attcagcctt gaggtcctga atgaactgct tgggagagag cggcttgtcc gtattgtatg
     1621 cgggacagag atcctgacag cgcccgcagc gtgtacaggc gtccaggtcg aagatctgtc
     1681 tccaggtaaa gccctctatg ctgttgatgc cgaactcttc ggcgttctcg aaatcttcga
     1741 tgggtgctat cgcgccccgt gccccctcgg gaacatcttc gacaccccgg aacatcatgt
     1801 tcaacgaaga ggtgatgatg tgaagcagtc tggagtgaac gatgtagcca agaagaccga
     1861 aggagatcac catgtgcacg taccagaaga ccttatgggt cacttcgatg gattccttgg
     1921 gcatgccggt gaacatgtaa gccgttacat agcccacgaa actccacttc tcatagtcgg
     1981 gcatcgaatg ggcgatcctc acgccttccg ccaggaaccc cgttacgaga acgacaagta
     2041 tccagaccag cactatggca tcgtccggtt tgttgtcgag cctcgtcggt ttcgtgacgt
     2101 agcgccggta catggccatc agtatgccga taatggccat gagacccgcc aaatccagaa
     2161 agaacgagaa gatcaggtaa aaaccgccct tcaggaactg caccttgaac agaaggtccg
     2221 tgaagtccgc ctggatcgcg acaagggccg tcccaacggt gaggatcagg aatccccaga
     2281 agatgaagaa atgcatccat cccggaaaag cttcgcgcag gatggatttg tggaagatcc
     2341 cgttcgcgat gaaatatcct atcctctcgc caagcctctt ggtgaattgc attggggatg
     2401 gctttcctac cttccacatg gcgtagcgct ttttcagtcc catgatcaga acaatggcaa
     2461 caatgaccaa gaaggcataa ctgacatatt gaccagtatg tcctacgttc cacatgatct
     2521 ctcgtccgac atcgaccata aactctgcct cctgaggatt ttttttcgta tattatcatt
     2581 tcaaaagccc agtaaaatca acaaaaatgt gattgatttc acgaaccgcc ttcgattggt
     2641 ctctaaggtc tcccgcgctc atgcaggcaa gaacacaggc aatccgccgt ttgacggcac
     2701 gtggaaggag attttgcttg acattttttc accctctttg gtattgtaca ccctataata
     2761 ccgaacaaag gggctttatg agaagacggc tgactctctg cgtactttgc atcagcatcc
     2821 tgttcctgac aatccctctg gtcgctaacg cctcccaggt cacccataaa gtaaaacgcg
     2881 gtgacagtct tcacaacctg gcgaaaaagt accatgtctc cgtgagccat cttaagaacg
     2941 tcaacgggct tcgctcaaac aagctctctc tcggtcaaac gatcgtcatc aagaacaatg
     3001 aagaaaaccc tgctgttaga gagacgcgca aggggccgtc ttcgaaagct ataagggccc
     3061 acgtgaggca ggaagaaccg gcagaacccg tcatctccga gaacgatgat gaattcgtgg
     3121 aatacagggt gaagcgaggc gacacccttg acaagatagc cagcaagttc aatgtcgaaa
     3181 aagatgatct catcgcaacg aacgatctca catccaggaa aagcagaaag ctctcccccg
     3241 ggaagaccat cctcattccg agagtgatgg aagatgacgg cgacgacggc gataccatcg
     3301 tgtcgttcaa gaatgccccg gtaaagcctt ggaagaacag tgatgagaaa tacatgctcg
     3361 taaaggtggc gaaaagtttc atgggtgctc cttacaaata cggcggaaac accctccgcg
     3421 gtttggactg ttccgcgtat gtgaaaaaga tatacgagat cttcgatgtc gaacttccga
     3481 gaagcgcccg cgaacaatac aaggtcggga acaagatctc cagagaggag ctgtccgtcg
     3541 gcgaccttgt cttcttcaag acacggcgct acgccaagta tcccactcat gtgggcatct
     3601 tcataggcga tggcaatttc attcacgcct cttcgggcaa aagacgtctc ggcgtgaaga
     3661 tagacacact tacttccggt tactactccg gggcctatat cggcgctaca cgcatcaagg
     3721 gaaatgtcga tgcaccaacc agccggggcc ctgaagcgac gcagcccgtg gagaaaccca
     3781 gcaacaactc ctgatatccc atgtgagaaa agaacatttt ctcgtaggcc tttcaatagc
     3841 cctctatctg ttcggtcacc tggcgctcgt atggaatctt gaaccggcca tcagcttctt
     3901 ctatgtcacc tcctggtggt cgtacataat ccttctcgat tccttcgtat cctggaagac
     3961 cggggagccc ctcttcctca accgttccct gccggccgtg ataatagtct catgcggtta
     4021 ctggtgcgcc ttcgaactcc tcaacctgcg tatcgagaac tggttctaca tcaacgtccc
     4081 tcacgctatc gcgctgcgat atgccggata cctccttgcc tacggcaccg tcatcccggc
     4141 tatcggtctc acggctgcca tcgcatcccc gctcctcggc cgtatcaggg tgcggcccgt
     4201 cacgaccccc cgcagttatc cggcccgggc catatcctgc ggcatcgccc tcttcctgct
     4261 cacgctgatc tttcccggtt atcttttcgg cctcgcctgg gtgttcgcca ctcccctcat
     4321 cgacggggtc aattaccgtg cgggataccg gtccttcatg ggcgacctcg agcgcggtca
     4381 gatcggtcgt cttctcggcg ccctggcttc cggactcgta tgcgggctcc tgtgggaaac
     4441 gtggaattcc ctttcacccg tcaaatgggt ctataccgtt ccattcttcg agcgaatgaa
     4501 gatcttcgag atgcctcttc ccggatatat cggcttccct gtgttcggcg tggaaacggt
     4561 ggcattcctc gacctgttcg agggcctgcg gcgcaagaag agcttggccg ccctcacgct
     4621 ctgtggcgct ctcctcacca gtgtcctctc cttctccttg attgacaggc acaccgtctt
     4681 ttcccgcacc acccccgtgg aacagcttct ctttctcagc cggaagagca aagatgccct
     4741 gctcaccaaa ggcgtaagga cgaacctgac cgtcgacaca agcctgctcg gtcctggtga
     4801 agctcagaga atgaggctcg tcaacctcag gggcctcggc tatgagaact acctcaaact
     4861 ggaggtccac ggcatcacca gcgtggacgg cctcgccagg ctcgacgaaa cgaggctcgc
     4921 ccagatgctt ggcgagaaga accttctgag gatacacatc tatcagtccg cggcacggcc
     4981 acattgagtg ttgacatggt cgacacatct gtggtgaaat agcagccaga aatggcgaga
     5041 aaaaaagaga aagagctcat ccacaagcct gacatgcttg tcacggcctt cgagtcggtt
     5101 tccctgtaca tcagagataa caggaggcag tgcgtctacg ggctcgttgc ccttctcatc
     5161 atagtcgccg ccgtcgcttc ctacatcgtc tacgcgaacc accaggccca gaaggtccag
     5221 tacaagctga cccagggcat aacggcctgg gagacgtacg aacagacaag ggctgccggg
     5281 gagatagaca aggcggaaga catcttccag aagatcgcct cgggcgcgag cggcaaaccc
     5341 cgctatatct caaagctata tctggcgaac atcaacatgg cccgcggcaa gcgggatgaa
     5401 gccctgaaac tctaccagga cgtctccagg aactcatcga acaaaacgct cacctatctc
     5461 gccgacaacg cgatcaaaac cctcgaaaag aagtaaagaa ccgtctcagg tcgcaggaga
     5521 aagacagttg cgggttccgg gttgcgcgtt tcgagtcaaa ggctccggtc ttgcccgaac
     5581 tgcagactat gcagtatccg aattgggtct tttcccttga gacttgagac ctgagacctt
     5641 ccctaggagt agagcgcttt gaccggttcg tacttggttc cttccatggc ggggtagata
     5701 ccggcaagaa ctccgatgaa catgctgatg gcgagggcca ggggaatgtt cattgagaag
     5761 actatgggga agcctccgat gatatcgacg atcaccgtta tcagaagtcc cgcgaggatg
     5821 ccgccgatgc ctcccacgag ggcaacgatg accgattccg acaggaactg tattatgatg
     5881 tccctcttct tcgaacctac gacccgtctg atcccgatct ccatcttcct ctccgatatg
     5941 gaaaggagca tgatggcgaa gataccca
//
LOCUS       DAOPOA010000040         5963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_14579_length_5963_cov_4.250508, whole genome shotgun sequence.
ACCESSION   DAOPOA010000040 DAOPOA010000000
VERSION     DAOPOA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14579_length_5963_cov_4.250508"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1956
                     /locus_tag="PKJ17_01520"
     CDS             <1..1956
                     /locus_tag="PKJ17_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD74675.1"
                     /translation="VVGAGPAGLACANDLALEGHDVTVYEALPEPGGMLRYAIPEYRL
                     PKEELKGEIDYIRRLGVEILCGVDVGGEKTAAVIRDACDALFVATGAPKGLPLGVEGE
                     TLEGIVDGIRFLRGVNRGEHASPGDRVAVIGGGNTAVDCARTAKRLGSASVTVVYRRT
                     REEMPAAGEEIEALLHEGIGIEFLAAPVRFRGDGGKVAEMECVRMELGEPDESGRRRP
                     VPKAGSEFTIPVDSVITALGQAVETSFLESLGVALGRGGTVTIDTATGATNIEGVFAG
                     GDVVTGPAYVIDAIAAGRKAARSIGFYLRGEAPAGDEERGTPQRLSEEQRGVLTGRIE
                     HRPRMAMPEEPVDERVTDFREVGLGYQSADAMAEARRCIAGQVEGCIGCGECLRRCEA
                     KAIDYSMRDELVEMYFDGIVVAPGFDLYDPTEKTEFGYGRLKGVITGMEFERLTSVTG
                     PTGGDIVMNGKTPKRFYFIQCVGSRDRQSGARFCSRVCCMYTAKHACIVKDRIRDARI
                     YISYIDVRTYGKGYEEFYKSTQENGAMYIRGIPGEVTQGENGLLVRVEDMLSGELHEI
                     EVDVVVLATGVRPRAGTGEMCEMMSLERDEYGFISTSVVEPSRTSVDGIFVCGMASGP
                     RDVPDTVASGGEAASRCMEYVKESASS"
     gene            1953..3623
                     /locus_tag="PKJ17_01525"
     CDS             1953..3623
                     /locus_tag="PKJ17_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOD74676.1"
                     /translation="MKTGIFICHCGHNIKHTVDVKRLSEFFGKLPNVVVSQDYPFVCS
                     EPGQDLISESIEKYGLDRVIVASCTPSLHGEMFKDVLRKKDLNPFLLRRVSIREHCSW
                     VGDDIEENTEKAKRLILSGLYGVAHQVPLEEKMVEVNKTALVIGGGVSGLSAALFLSK
                     MGMQVYLVEKEPQLGGVVREIRNVWPARTSGAKITGDMEKELRSRQNVEILTSSELTG
                     FEGFFGNYEATLDTPAGERKIVIGGAVAAIGFAPFDPAIKPELQYGKDPRIVTTLDLE
                     RAGELALPENPRVAILHCIGSRDEQIGRPYCSRICCINALRVGEAIRERYADSYVESF
                     YMDMRAHPRGAEEFFEDTQEKGVLFTRGNIGEIVPAPTGVLLKGEDTLLGETFEREFD
                     LVVLSIGMSYPVDSQKLATLLKISLDKDRFFLEAHIKLRPFDTAVKGIFIAGSCSGPK
                     DMEESINHGRASALKLYGLLNLGYAFVDPFISFVDPKRCSGCRMCEQACFSKAIKYDE
                     EKKVVHVEEAACQGCGLCNATCPSSAISLRGYYDAILDDEISAFLEAI"
     gene            3657..4064
                     /locus_tag="PKJ17_01530"
     CDS             3657..4064
                     /locus_tag="PKJ17_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOD74677.1"
                     /translation="MEDRTAKNPEIVGFACTYCTFKASEMAGSLRMKYPEGVKVIQVP
                     CSSRVDPAFVIKAIFEGADGVFVAGCHPGDCHFIKGNYYTRRKIAALKELFDAFGMKD
                     RLRLFWVSASEARRFVEKVTEFYNDIKEKKNAG"
     gene            4054..5058
                     /locus_tag="PKJ17_01535"
     CDS             4054..5058
                     /locus_tag="PKJ17_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD74678.1"
                     /translation="MQGKKIKNGALESALNTFLAEKEYLVMGFEKDASNVYHRVFKDS
                     LDNYALMSPVFATNGALYLRRLFAKGAQIILMLRPCEIRSYVELVKLTQIEPENIIAV
                     SVDCFGTVSSKTEGDIPAGEALKGLTKDAEKARWACANCRERRGVVGDAGIRIDANGD
                     YWALPYTDKGQAFLSLLEGETSEAPAQMAIGPAEPRPAFQIDMKEFTKDFSKCIMCMN
                     CRDMCPVCYCVDCVFNGDEYLPKGDALFNKVFRAGSTGMPRGKELFHLIRMYHVSQTC
                     VGCGACEEACPQGIPLTKYFKGVSERLQGMFSYMSGRSKDEIIPYLTFIEEELKDAED
                     "
     gene            5045..5503
                     /locus_tag="PKJ17_01540"
     CDS             5045..5503
                     /locus_tag="PKJ17_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOD74679.1"
                     /translation="MPKTEQALELTDYGRQDFAVCLGCKICASVCTVNDLSPGTNPQE
                     MLQRLFLGQDVAADEPLVRFCTGCYRCTGACPWEIRIPEVIRALRHELGTESPFEKAF
                     KGSVSLFGRVYEPYVLMKAVPFLLKGGYMKHVTRWMEYVGFHLPHKVKGM"
     gene            5505..>5963
                     /locus_tag="PKJ17_01545"
     CDS             5505..>5963
                     /locus_tag="PKJ17_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOD74680.1"
                     /translation="MEYGYYPGCSLTGSAHRLDKGIKKVFAKQGHTLREIPDWNCCGA
                     FEYGDRKELTGFSKRNLEKARGHFSEIVAPCPACYKNLKEADEEREFAVTHPLDLFDE
                     AFFAAMKQARDLTGQVFTPYYGCILLRPKETAIQNTNIMEETIARFGGEVS"
BASE COUNT         1221 a         1714 c         2002 g         1026 t
ORIGIN      
        1 gtggtcggcg ccggcccggc ggggcttgcc tgcgccaacg acctcgccct ggaaggccac
       61 gacgtgaccg tgtacgaggc gctccccgag ccgggcggca tgctccggta tgccattccc
      121 gaatacaggc tcccgaagga ggagttgaag ggcgagatcg actatatccg caggctcggg
      181 gtggagatcc tctgcggcgt ggatgttggc ggagaaaaaa ccgccgccgt gatccgggac
      241 gcctgtgatg ccctcttcgt ggcgacgggt gccccgaagg gtcttcccct cggcgtggaa
      301 ggcgaaacgc ttgaagggat cgtcgacggc atcaggttcc tgcgtggtgt caacaggggc
      361 gagcacgcgt cccctggcga ccgtgtcgcg gtcatcggcg gagggaacac ggccgtcgat
      421 tgcgcgcgga cggcaaagag gctgggcagc gcgtccgtca ccgtagtcta tcgccgtacc
      481 cgggaggaga tgcccgcggc cggcgaagag atagaggccc tgcttcacga agggataggc
      541 atcgaattcc tcgccgcgcc ggtccgtttc cggggggatg gcggaaaggt cgcggagatg
      601 gagtgcgtcc gcatggaact gggcgagccc gatgagagcg gccgccgccg gcccgtgccg
      661 aaggccgggt cggagttcac gatacccgtg gattcggtga taacggccct cggtcaggcc
      721 gtggaaacct ccttcctcga atcccttggt gtcgccctgg gcaggggggg cacggtgacg
      781 atagacaccg cgacgggtgc caccaacatc gaaggtgttt tcgcgggcgg ggacgttgtc
      841 accgggcccg cgtacgtcat cgatgccata gcggccggaa ggaaggcggc gcggtcgatc
      901 ggcttctacc tcaggggcga ggccccggcg ggcgatgagg agcgcggcac gccgcagcgg
      961 ctttcggaag agcagcgcgg ggtcctcacc ggccggatcg aacaccggcc gcggatggcg
     1021 atgcccgaag agccggtgga cgagagggtg accgattttc gcgaggtggg gctcggctac
     1081 cagagcgcgg acgccatggc cgaggcacga cggtgcatcg ccggccaggt cgagggatgc
     1141 atagggtgcg gggaatgcct ccgtcgctgc gaggcaaagg cgatagacta ctccatgcgg
     1201 gacgagctgg tggagatgta cttcgacggg atcgtcgtcg ccccgggttt cgacctctat
     1261 gacccgacgg agaagacgga gttcggctac ggcaggctga aaggtgtcat aacggggatg
     1321 gagttcgaga ggctcacctc ggtgacgggg ccgacgggtg gagacatcgt catgaacggg
     1381 aagacgccga agcgcttcta cttcatccag tgcgtcggca gccgcgaccg ccagagcggt
     1441 gcccgattct gttcccgggt ctgctgcatg tacacggcaa agcacgcgtg catcgtgaag
     1501 gacaggataa gggacgcacg gatatacatc tcttacatcg atgtgaggac ctacggcaag
     1561 ggctacgaag agttctacaa gagcacccag gagaacggcg ccatgtacat ccgcggcatc
     1621 cccggagagg tgacgcaggg cgagaacggc ctcctggtgc gcgtcgagga catgctctcc
     1681 ggcgagctcc acgagataga ggtggatgtg gtcgtcctcg cgacgggcgt gaggccccgg
     1741 gcgggtaccg gggaaatgtg tgagatgatg tccctcgaga gggacgagta cggtttcatt
     1801 agcaccagcg tggtggagcc ttcgagaaca agcgtggacg gcatcttcgt ctgcggcatg
     1861 gcatcggggc cgagagacgt ccccgacacg gtggcctcgg gaggggaagc ggcgtcgcgg
     1921 tgcatggaat acgtgaagga gagtgcctct tcatgaaaac agggatcttc atctgccact
     1981 gcgggcacaa catcaagcac acggtggacg tcaagaggtt gagcgagttc ttcgggaaac
     2041 tgccgaatgt ggtcgtttcc caggattacc ctttcgtgtg ctccgagccg gggcaggact
     2101 tgatcagcga gagcatcgag aagtacggcc tcgaccgcgt catcgtagcg tcctgcaccc
     2161 cgtccctcca cggcgagatg ttcaaggatg tgctgaggaa gaaagacctc aaccccttcc
     2221 tgctgcgcag ggtgagcatc agggagcact gctcgtgggt gggcgacgac atagaggaga
     2281 acacggaaaa ggcgaagagg cttatcctgt cgggtctcta cggcgtggcg caccaggttc
     2341 ccctcgagga aaagatggtg gaggtcaaca agacggccct cgtcatcggg ggcggtgttt
     2401 cgggcctctc agcggcactt ttcctgtcga agatgggcat gcaggtctat ctcgtggaaa
     2461 aggagccgca gctcggcggg gtcgtgcggg agataaggaa cgtctggccg gcgaggacga
     2521 gcggcgcgaa gataacggga gacatggaga aggagctcag gagcaggcag aacgtggaga
     2581 tcctgacgtc ctcggagctt acgggcttcg aaggcttctt cggcaactac gaggcgaccc
     2641 tcgacacccc ggcgggggag aggaagatcg tcatcggcgg ggcggtcgcg gccataggct
     2701 tcgcgccctt cgatcccgcg ataaagcccg agctgcagta cgggaaggac ccgcggatcg
     2761 tgacgacact cgacctcgag cgcgcgggcg agcttgccct gcccgagaat ccccgggtcg
     2821 cgatcctcca ctgcatcggt tcccgggacg agcagatcgg caggccctat tgctcgcgta
     2881 tctgctgcat caacgccctg cgcgtgggag aggccatcag ggaaaggtac gccgactcct
     2941 acgtggagtc cttctacatg gacatgaggg cccatccgcg gggggccgag gagttcttcg
     3001 aagacaccca ggagaaggga gttctcttca cccgggggaa tatcggggag atcgtacccg
     3061 caccgacggg ggtcctcctc aagggtgagg acacgcttct cggcgagacc ttcgaacgcg
     3121 agttcgacct cgtggtcctc tcgatcggga tgtcctatcc cgtggacagc cagaagctcg
     3181 ccacgcttct caagatcagc ctcgacaagg acaggttctt tcttgaggcc cacatcaaat
     3241 tgaggccttt cgataccgcc gtcaagggta tcttcatcgc cggctcctgt tccgggccca
     3301 aggacatgga ggagtctatc aaccacggga gggcctcggc gctgaagctc tacggccttc
     3361 tgaaccttgg ctacgccttc gtggacccct tcatctcctt cgtggacccc aagcggtgca
     3421 gcggctgccg catgtgcgaa caggcctgct tctcgaaggc gataaagtac gacgaggaaa
     3481 aaaaggtcgt tcacgtcgag gaggccgcct gtcagggctg cgggctttgc aacgccacct
     3541 gcccgtcatc ggcgatcagc ctgaggggct attacgacgc catcctcgat gatgagataa
     3601 gcgcatttct ggaggcaata tgacctcgaa cgacgtcaag ggtagagggg aagtacatgg
     3661 aagatagaac ggcaaagaac ccggagatcg tgggttttgc gtgtacctac tgcacattca
     3721 aggcctcgga gatggcggga agcctgagga tgaagtaccc cgaaggggtg aaggtgatac
     3781 aggtcccctg ttccagcagg gtcgacccgg cctttgtcat aaaggccatc ttcgaggggg
     3841 ccgacggcgt tttcgtcgcc ggctgccatc ccggcgactg tcacttcatc aagggcaact
     3901 attatacccg ccggaagata gcggccttga aggagctttt cgacgccttc ggcatgaagg
     3961 acaggctccg gctcttctgg gtgagcgcct cggaggcgcg gcgtttcgtt gaaaaggtga
     4021 cggaattcta caacgacata aaggaaaaga aaaatgcagg gtaagaagat aaagaacggc
     4081 gcgctcgaaa gcgctctcaa cacgttcctc gcggagaagg aatacctcgt gatggggttc
     4141 gagaaggacg cgagcaacgt ctaccaccgg gtcttcaagg acagtctgga caattacgcc
     4201 ctcatgagcc cggtcttcgc gacgaacggc gccctctacc tgagaaggct cttcgcaaag
     4261 ggcgcgcaga tcatcctcat gctccggccc tgcgagatac ggtcctacgt ggagcttgtc
     4321 aagctgacgc agatagaacc cgagaacatc attgccgtct ccgtcgattg cttcgggacg
     4381 gtctcgtcga agacagaggg cgacatcccg gccggagagg cgcttaaagg gctcacgaag
     4441 gatgccgaaa aggcgcgctg ggcctgcgcg aactgccgcg agcggcgcgg tgtcgtcggt
     4501 gacgcgggga tacgcatcga cgcgaacggg gactactggg cccttcccta cacggacaag
     4561 ggacaggcgt tcctttcgct tctggaaggc gagacctccg aggcgcccgc gcagatggca
     4621 atagggcctg ccgaaccccg gccggccttc cagatcgaca tgaaggagtt cacgaaggac
     4681 ttctcgaagt gtatcatgtg catgaactgc cgcgacatgt gtcccgtgtg ctactgcgtt
     4741 gactgcgtct tcaacggcga cgaatacctg cccaaaggcg acgcgctctt caacaaggtc
     4801 ttccgcgccg ggtcgacggg gatgccccgg ggcaaggaac tcttccacct catccgcatg
     4861 taccatgtct cccagacctg tgtgggatgt ggtgcctgcg aggaggcctg ccctcagggg
     4921 ataccgctga cgaagtactt caagggcgtc tccgagaggc tccaggggat gttctcctac
     4981 atgtcgggac gcagcaagga cgagatcata ccgtacctta cctttataga ggaagaactg
     5041 aaagatgccg aagactgagc aggctctgga actcaccgat tacggaaggc aggacttcgc
     5101 cgtgtgcctg ggatgcaaga tctgcgcctc ggtgtgcacc gtgaacgacc tgtctcctgg
     5161 cacgaacccc caggagatgc tccagaggct cttcctggga caggacgtgg ccgccgacga
     5221 gccgctggtg cgcttctgca cgggatgcta ccgctgcacc ggcgcctgcc cctgggagat
     5281 acgcataccc gaggttatca gggccctgcg ccatgagctc ggcacggaat ctcccttcga
     5341 aaaggccttc aaggggtcgg tctctctctt cggcagggtc tacgagccct acgtgctcat
     5401 gaaggccgtg ccctttctct tgaagggggg gtacatgaag cacgtgacgc ggtggatgga
     5461 atacgtgggt ttccacctgc cccacaaggt aaaggggatg taacatggaa tacggctact
     5521 atcccggctg ctcgctgaca gggtcggcgc acaggctcga caagggcatc aagaaggtct
     5581 tcgcgaaaca gggccacacc ttgagggaga tccccgactg gaactgctgt ggggccttcg
     5641 agtacggcga caggaaggag ctcacgggat tctcgaagag gaacctcgaa aaggcgcggg
     5701 ggcatttcag cgagatcgtc gccccctgcc cggcgtgcta caagaacctc aaggaggcgg
     5761 acgaggagcg ggaattcgcg gtgacgcacc ctctcgacct cttcgacgag gccttcttcg
     5821 ccgcgatgaa acaggcccgc gacctcaccg gccaggtctt cacgccctac tacggctgca
     5881 tcctccttcg gccgaaggaa acggcgatac agaacacgaa catcatggag gagaccatcg
     5941 cccgcttcgg cggggaggtg tcc
//
LOCUS       DAOPOA010000041         5956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_14604_length_5956_cov_3.697848, whole genome shotgun sequence.
ACCESSION   DAOPOA010000041 DAOPOA010000000
VERSION     DAOPOA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14604_length_5956_cov_3.697848"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKJ17_01550"
     CDS             <1..199
                     /locus_tag="PKJ17_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74681.1"
                     /translation="LKDRDIPVVFLSSHMGEEIMEETKKITPYGYVVKDGDMTALGIR
                     VKKAMKLHETRGGLKEKKKPL"
     gene            367..603
                     /locus_tag="PKJ17_01555"
     CDS             367..603
                     /locus_tag="PKJ17_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74682.1"
                     /translation="MKFVFSAIFLFMFSLTAAAAADKPASDCIDKCEAAKKSCMAQYT
                     KSDSTSGKYVTPEGHKICWQGYHDCKKQCPKGGK"
     gene            complement(621..1145)
                     /locus_tag="PKJ17_01560"
     CDS             complement(621..1145)
                     /locus_tag="PKJ17_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOD74683.1"
                     /translation="MVLAALGLTALLQNEKIRAAVNYFGTATIVFFCLHTILQVFAVD
                     MIPELDLMIKVRSASTFVHAAILTASNPLSLLLWAGVFSARITQDRLSVNEAGVFGLG
                     MLLSDCLSLNIIAFIGCFTSTLLNSFAMAVLNIAVGIFLIYLAVAKFIANRKKVSIEN
                     NNIADKPGVFFRFS"
     gene            1195..1716
                     /locus_tag="PKJ17_01565"
     CDS             1195..1716
                     /locus_tag="PKJ17_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOD74684.1"
                     /translation="MQGKEIHPGESDKQLAAVCGLYCEACSLFIATKEDPERLKGIAA
                     RYQVSEEAARCCGCRSEKRGPYCTVCTMCVCAARRGIDFCIECAEYPCDDLRRFQSER
                     PHRMELLDDLERIGAVGYKMWLREMGDNYTCPRCGTVNSAYDLKCRKCGEEPSCAYVA
                     KHKEEIERFLKTL"
     gene            2100..2558
                     /locus_tag="PKJ17_01570"
     CDS             2100..2558
                     /locus_tag="PKJ17_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HOD74685.1"
                     /translation="MNPVSLRDVVDALDMASDTLSSYLNRKTGEIVSLTEDTMLMVDD
                     DDDLDGISEWEREEILRARDVRDSDDYIGLPGRYEIDEYRIMEDFCYSLEDEGLKEDL
                     LYTIDGRGAFRRFKDAIHRLGIEKDWFKYRDGRLKELAVDWLKANGIPFI"
     gene            complement(2686..2853)
                     /locus_tag="PKJ17_01575"
     CDS             complement(2686..2853)
                     /locus_tag="PKJ17_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74686.1"
                     /translation="MEEKDSKKIPEHNCNEHVRYDGGQKINRDGVRIWVETYYCRICG
                     RRLDIKEEILD"
     gene            complement(2870..5104)
                     /locus_tag="PKJ17_01580"
     CDS             complement(2870..5104)
                     /locus_tag="PKJ17_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOD74687.1"
                     /translation="MTLSDLCELVARYYPDGERTLPRILFVWAMHDEDGSLAQLLAQA
                     KTKVSVFSLAVEGFIEDRVEEDRRLLTRTVIETRRKPVTAYDVLSTLCASPDHRIYRA
                     LKSAGIDMTALAICAKHAGYAAHRKTILAEQRAVRNDGVLLQYGRDLTKSAENGDFDD
                     LSDRPDEIDRLFEVLLRKRKRNAMLTGPAGVGKTALVELLSRRIARHLAPARLFRTRV
                     YEISMGSLLAGTTLRGMFEERVSKVIEALQACQPAILFIDEAHLVWGAGRAEGAPMDA
                     ANMLKPILAGSKVSVIGATTTGEYHRYITRDAALARRFQQIKIEEPGREATLAILARH
                     ARGLENHHGVRIGADVLSEAWHATQRHLSNRTQPDKSVDLLDSAAGLVEREGRNEMGT
                     EDLYRVLSRQTGRMMSALGEGRTRDLTGLEDSLNRQVIGQEEAVRRVVSTLVSRRYGE
                     VAPVRPLGTFLFSGATGVGKTELARTLAREYFGSPKDFLHIDLAEYPGQDGVHKLIGT
                     PYGSAFGTADGLLTRWLEERSSGVILFDEIEKAHPDVHRLLLGMLDEGRVTSGMSERF
                     DIRRCVIILTTNVLAHRDVDRVKLGFSTGDDPSDPFALLAKHFPEEFLGRLDEIIVFR
                     TPPAPVLRRIMAQKLREALARLKERGVFLIYDEDRLLDHLLAGTGRPWAGARDIARLI
                     ERKLIQPIARRLIDCDTQDWICIELTESFYRSGALNPLPLLKRPGPPPDRPDTPASAA
                     DARQ"
     gene            complement(5101..>5956)
                     /locus_tag="PKJ17_01585"
     CDS             complement(5101..>5956)
                     /locus_tag="PKJ17_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74688.1"
                     /translation="LLIENQRIRNAHTRREWLALFGKEEIGLREAALILERCDMRLNL
                     KKVNPDWSREDIEEYIEREEAQRQLELTRLKTDAALAPHLVRISGETGAGGKAVEADR
                     YLKECKTVLRQIRRLLHPDRLMHHPSYAHLTEGQKERLEEMLLSALEVRPEELGYPEG
                     YILHDMRSLEGLKSALTRVEAILGNPGVEIDERLTIQGETLPEKLTWLQREVRLLEDE
                     VLAAKAELQALFEDEDRVRERAILGTPAGHEKVSADLAARAGELRRDAERLEQAFNAL
                     FDGEGEPR"
BASE COUNT         1199 a         1687 c         1816 g         1254 t
ORIGIN      
        1 cctcaaggac cgtgatatac ccgttgtgtt cctttcctcc cacatgggag aggagatcat
       61 ggaagagacg aagaagatca caccctatgg atacgttgtc aaagacggcg atatgactgc
      121 cctcggcatc cgggtgaaga aggcaatgaa actccatgag acccgcgggg gattgaaaga
      181 gaaaaagaag cctctttgaa aatgccgtga aaggtctcag aagtgccctc tgcccccaat
      241 accgttcgat tttgtttgac atacaaacgg gcatgagtga tattttgcgc gacagatgcg
      301 gggaagtctc ttcgtgaatg tgtcgccgac taacaggtcg cgcaaggcat ctcaaaggag
      361 gatcgaatga aatttgtttt ctctgcaatt ttcctgttca tgttctcgtt gacggccgcg
      421 gcggcagccg acaaaccggc gtcagactgt atcgacaaat gcgaggcggc caagaagtcc
      481 tgcatggccc agtacacgaa gtcggacagc accagcggaa agtacgtgac ccccgaaggc
      541 cacaagatat gctggcaggg ctaccacgac tgcaagaagc agtgccctaa agggggcaaa
      601 tagacctctt cagcaaggct tcaagaaaac cggaaaaaca ctcccggctt gtctgcgata
      661 ttattgtttt cgatggagac ttttttgcgg ttggcaatga attttgccac tgcaagatag
      721 atcagaaaga tgcccacggc tatattcaat accgccatgg caaagctatt caacaaggtg
      781 ctggtaaagc agccgatgaa tgcgatgata ttcagagaca gacaatccga cagtagcatg
      841 ccaaggccga atactcccgc ctcattaact gatagtctgt cctgggttat cctcgcagag
      901 aatactccgg cccaaagcag gagcgatagt ggattggagg cggtcaggat tgcggcatgg
      961 acaaaggtac tggccgatct tacctttatc atcaagtcca gttccggtat catatcaact
     1021 gcaaagactt gcaggatcgt gtgcagacag aagaatacga tcgtcgccgt cccgaaatag
     1081 ttgacagccg cccttatctt ctcattctgc agaagggcgg tcagtcccag agcggcgaga
     1141 accatcagga acacatcaag tattcaggca accggcagac gaaaaggagc tattatgcaa
     1201 ggaaaggaaa tccatcccgg agagtccgac aagcaactgg ccgctgtgtg cggtctttat
     1261 tgcgaggcct gttccctgtt cattgccacg aaggaagacc cggaaaggct taagggaata
     1321 gcggcacggt atcaggtttc ggaagaagca gccaggtgtt gcggatgccg gtcggaaaag
     1381 cgagggcctt actgtacggt atgcacgatg tgcgtctgcg cggcccgtcg ggggatcgat
     1441 ttctgcatcg aatgcgcgga gtacccctgc gatgacctga gacggtttca atctgaaaga
     1501 cctcatcgga tggaactctt ggacgatctg gaacggatcg gagccgtcgg gtataagatg
     1561 tggttacggg agatgggcga taactacacc tgtccgcggt gcgggaccgt gaattccgcg
     1621 tacgacctca aatgcaggaa atgcggtgaa gaaccgagct gtgcctacgt ggccaagcac
     1681 aaggaagaga tagagcggtt cctgaagacg ctgtgaccgg aaaagacaat tattgacctc
     1741 tcgaatctcg ccgacgacgt gactcaccgc gtggatgggt ccaacgtgtt gtgcaataat
     1801 ctacctatta cttccattct gcgcctcatt atggaattat aatacccatt ctgcgcctgt
     1861 acggatgatt agtcaagaga attccgaatc acagtggact ccaggggcat cctgggggaa
     1921 acttcggggc ccttcggatt ctcgcatctt tgctcgaaga tcgccggctt cagtgtgacc
     1981 gggacagctt cgggttcaaa aagagtctca ggaggaagtc acctatgaac aggggtgggt
     2041 gtatagagaa aatatttgaa tgtccggcgc cctttcggta gcataaggat atgaccaata
     2101 tgaatccagt atccctcagg gacgtcgttg acgcgctgga catggcgagc gataccctgt
     2161 cttcatacct taacaggaag acgggagaga tcgtgagtct caccgaagac accatgctga
     2221 tggttgatga cgacgatgat ctcgacggga tctccgaatg ggagcgggaa gagatcttga
     2281 gggcgaggga tgtcagggat tccgatgact atatcgggct tcccggcaga tacgagatcg
     2341 acgaatatcg catcatggag gatttttgct actctcttga ggacgagggc ctgaaggaag
     2401 acctcctcta caccatcgac ggccgcggag ccttccgccg gttcaaggac gccattcacc
     2461 gcctggggat cgaaaaagac tggttcaaat atcgcgatgg gaggctgaag gagctagcgg
     2521 tcgactggct gaaagccaac gggatcccct tcatttgaac tccggggaca tcccttcaca
     2581 ttttcacatt ctcaaaatac tgcaagtacg gagactgcca gatagccttt ccatcgtctt
     2641 tcggttctaa cgttcaacag cggttcaagc cattcaaacc gtccctcagt ccagtatctc
     2701 ttcttttata tccagacgcc tcccgcagat gcggcagtaa tatgtctcaa cccatattcg
     2761 cacgccgtct cggttgatct tctggccacc atcgtagcgg acatgttcgt tgcagttgtg
     2821 ttccgggatc ttcttgctgt ctttctcttc catgatggta cctcctttgt tattgccggg
     2881 catccgcggc ggaagccggg gtgtcggggc gatccggcgg tgggccgggg cgtttgagga
     2941 gcggcagcgg gttcagggcc cccgaacggt agaaggactc cgtgagctcg atgcagatcc
     3001 aatcctgcgt gtcgcagtcg atgaggcggc gggcgatggg ctggatgagc ttgcgctcga
     3061 tgaggcgggc tatgtcgcgc gcgccggccc agggtcttcc ggtgcctgcg agaagatggt
     3121 cgaggagacg gtcctcgtca tagatgagga agacccccct ttccttgagg cgggcgaggg
     3181 cctctcgcag cttctgggcc atgatcctcc tcaggacggg cgccggcggc gtcctgaaga
     3241 cgatgatctc gtcgagcctt cccaggaact cctcagggaa gtgtttcgcg aggagcgcga
     3301 aggggtctga cgggtcgtcg cccgtggaga accccagttt cacccggtcg acgtcgcggt
     3361 gggcgaggac gttcgtggtg aggatgatga cgcagcggcg gatgtcgaag cgctctgaca
     3421 tgcccgacgt cacccttcct tcgtccagca ttcccaaaag aagcctgtgg acatcagggt
     3481 gtgccttttc gatctcgtcg aagaggataa cgccgctcga tctctcctca agccatcggg
     3541 tgaggaggcc gtcggccgtc ccgaaggcgg agccgtaggg cgttccgatg agcttgtgca
     3601 cgccgtcctg tcccgggtac tcggcgaggt cgatgtggag gaagtccttc gggcttccga
     3661 aatactcccg cgccagggtc cgggcaagct ccgttttgcc gacgcccgtg gcgccggaga
     3721 agaggaaggt cccgagcggt ctcaccgggg caacctcccc gtaccttcgc gaaacgaggg
     3781 tcgaaacgac cctgcgtacc gcctcctcct ggccgatgac ctgcctgttg agagaatcct
     3841 cgaggcccgt caggtccctc gttctgccct ctccgagggc gctcatcatc cttcccgtct
     3901 ggcgtgagag gacgcggtag aggtcctcgg ttcccatctc attcctgcct tccctttcga
     3961 cgagacccgc ggcgctgtcg agaaggtcca cgctcttgtc gggctgggtc cggttcgaca
     4021 ggtggcgctg tgtggcgtgc caggcctcgg agagaacatc ggccccgatc cggaccccgt
     4081 ggtggttctc gaggcctcgc gcgtggcgcg cgaggatggc gagcgtcgcc tcccttcccg
     4141 gctcttcgat ctttatctgc tgaaagcgcc ttgccagggc cgcgtcgcgg gtgatgtagc
     4201 ggtggtactc gcccgtcgtc gtggcgccaa tgacggacac cttcgatccg gcaaggatgg
     4261 gcttcaacat gttcgccgca tccatgggag ccccttccgc cctgccggcg ccccagacga
     4321 gatgggcctc gtcgatgaag aggatcgccg gctgacaggc ctgaagggcc tctatcacct
     4381 ttgatacccg ctcttcgaac atgccgcgga gtgttgtccc cgcgaggagg ctccccatgc
     4441 tgatctcgta caccctggtc cgaaagagcc gtgccggcgc caagtgccgg gcgatgcgcc
     4501 gcgacaggag ctcgacgagg gcggtcttgc cgacgcctgc cgggccggtg agcatggcgt
     4561 tgcgtttcct ctttctcagg agcacttcga agagacggtc gatctcgtcg ggccggtcgg
     4621 agaggtcgtc gaaatccccg ttttcggcag actttgtcag gtcccggccg tactggagga
     4681 ggacgccgtc gttgcgcacg gcgcgttgct cagcgaggat ggtcttgcga tgtgccgcat
     4741 agccggcgtg cttcgcgcat atcgccagcg ccgtcatgtc gatgcccgcc gacttgaggg
     4801 ctcggtatat gcggtggtcg ggactggcgc agagggtgga aaggacgtcg tatgccgtca
     4861 cgggtttcct gcgtgtctcg atgacggtcc gggtgaggag gcgccggtcc tcctccacgc
     4921 gatcttctat aaagccttcg acggcaaggg agaagacact tacctttgtc ttcgcctgcg
     4981 cgaggagctg cgcgaggctg ccatcctcgt cgtgcatggc ccagacgaag aggatccggg
     5041 gaagggtccg ttcgccatcc gggtagtacc gggccacgag ttcgcagagg tcgctcaagg
     5101 tcatcgcggc tctccttccc cgtcgaaaag ggcgttgaag gcctgctcca ggcgctcggc
     5161 atcacgcctt aactccccgg cgcgggcagc aaggtcggcg ctgacctttt cgtgaccggc
     5221 cggggtgccg aggatggccc gctcccgtac cctgtcctca tcctcgaaga gcgcctgcag
     5281 ctccgccttc gcggcgagga cctcatcctc gagcaacctc acttcccttt gcagccacgt
     5341 gagcttttcc ggcagggttt caccctggat ggtgaggcgc tcgtcgatct cgacccccgg
     5401 gttgcccagg atggcctcga cgcgcgtgag ggcgctcttc aaaccctcga gggagcgcat
     5461 gtcgtgaagt atgtacccct cgggatagcc gagctcctcg ggccggacct cgagggcgga
     5521 aagcagcatc tcctcaaggc gttccttctg gccctctgtg agatgcgcgt aagaggggtg
     5581 gtgcatgagc ctgtcggggt ggaggagcct gcgtatctgc cggagcaccg tcttgcactc
     5641 tttcaggtac ctgtcggcct ccactgcctt tccgcccgcg cccgtctccc cggagatcct
     5701 cacgaggtgg ggggccagcg ccgcgtcggt cttaagccgc gtgagctcga gctgcctttg
     5761 cgcctcttcc ctctcgatgt actcttcgat atcctcacgg gaccagtcgg ggttcacttt
     5821 cttgaggtta agccgcatat cgcaccgttc gagtatgagg gcagcctccc tgagtccgat
     5881 ctcttccttt ccgaagaggg cgagccactc gcggcgggtg tgggcgttcc tgatccgctg
     5941 gttctcgatg agcagg
//
LOCUS       DAOPOA010000042         5929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_14676_length_5929_cov_3.526728, whole genome shotgun sequence.
ACCESSION   DAOPOA010000042 DAOPOA010000000
VERSION     DAOPOA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14676_length_5929_cov_3.526728"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1771)
                     /locus_tag="PKJ17_01590"
     CDS             complement(125..1771)
                     /locus_tag="PKJ17_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOD74689.1"
                     /translation="MSLKIGTKFMLFISGVIAVAVVITASACLWEMRNDLIRQANRTM
                     DSRLNVFWELLNAKSQSSMEAAQSGQKQKVPFTIEDDRLVVGAYGLNGDTVLVDKIKE
                     FFGGTATIFMRDVRVSTNVLNPDGTRAVGTRLKGPVHDAIFQKKTSFRGSADILGKSY
                     FVAYDPIRGQNGEVIGVLYVGMPKGDYFAAFNRILMFVVIIGIILIGAVCVMSYFYMR
                     KLTLPLNECVSAAQRLAEGDLTVDIKAEDESETGQLLKGMQNMVLHWREIVHEVKEAT
                     DRIIGESRQLSVHADQMQQGSAQQATKSSQVASSAEEMSQTIVDVARNTNDIATSAGM
                     AVGIAKSGADVVSQSIEEVRSIAEVVRRSGQFVESLGRRSEQIGEIIVLINDIADQTN
                     LLALNAAIEAARAGEVGRGFAVVAEEVKKLAEKTAAATLEIGQTIEGMRSEVENATRA
                     MEEADVKVGSGVELVSRTGGSLGEIVSSAEGLQTMVMQIASATEQMSAASEEINREIV
                     DIAAVTNETSRSSEMTAASASSLLGLSDRLQNLMGRFRVS"
     gene            1967..2545
                     /locus_tag="PKJ17_01595"
     CDS             1967..2545
                     /locus_tag="PKJ17_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HOD74690.1"
                     /translation="MRCDNCGNESPEGSRFCIKCGREFGASGSGITVCPHCGVQIAPG
                     SLFCSACGKSIGAPQGEVNQGRTSQPPLSEPPTRPLTSNIALDVILSVITCGIYWFFW
                     QARQMRAINHLLGQERYSFWLWFFLTLITCGLYNIYYEYYMAQGIVEAQDSRGYLRSK
                     DLPVLSLILTIIGLNIVTDAIQQNEINKFFGK"
     gene            2574..2951
                     /locus_tag="PKJ17_01600"
     CDS             2574..2951
                     /locus_tag="PKJ17_01600"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOD74691.1"
                     /translation="MASAFALLLLVLAVVHATGILPFGTLAGILPVFCPFELLTGIPC
                     PGCGMTRAILCLIAGNPSDALLYNPFSFFLLTLVTASILPRRWLERIPTGIRSVLPRA
                     YAVILGLVITFWIFDRLLPALTA"
     gene            complement(3154..3885)
                     /locus_tag="PKJ17_01605"
     CDS             complement(3154..3885)
                     /locus_tag="PKJ17_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD74692.1"
                     /translation="MNIRGPFYFITKKTGKAIWDYRMLSDGDKVIIAVSGGKDSLCLL
                     KIMQERLKFVPINYELIACHVDMGFPWVNADMLADYFEKEGLSYIIASAPEDWTGDIE
                     NLDCFWCSWNRRKAVFDVAADIGANKIAFAHHMDDIIETMLLNLFFQGEIGTMMPYQE
                     MFDGELAIIRPLAYVEEKELARLATILNLPVIASECPRGDVSKRSLVKGIVADLKKHN
                     RNVKKNIFRSLAKIRQEYLPEKAPG"
     gene            complement(3882..4427)
                     /locus_tag="PKJ17_01610"
     CDS             complement(3882..4427)
                     /locus_tag="PKJ17_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOD74693.1"
                     /translation="MKNAVILFGSPRKKGNTVELARAVSATLKERGWGVRMLYLNDLN
                     IRPCQGCYVCLPGGVCKINDDMKDVRKYIMESDLIVYATPIYWFGPSGQLKLVMDRSI
                     AFMDENYGSRIEGKKAITLTTFADKDMDSCRPAIDIFQKTFDLLKVAYAGQVNAPGCE
                     PEGGVRQEYIDKARALAESLA"
     gene            4870..5265
                     /locus_tag="PKJ17_01615"
     CDS             4870..5265
                     /locus_tag="PKJ17_01615"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HOD74694.1"
                     /translation="MSNIFLGIITLAAVVLVVYVVYMILNLKRTIASLERFVETTEAT
                     LQPALEELQATLKSVRKITDDVGTLTDDIRHISGSVRNVGEAVDQVSYAVKSAAQASV
                     SETKGLKAGIRAGVSYFLRNACLRGGTQQ"
     gene            5282..5584
                     /locus_tag="PKJ17_01620"
     CDS             5282..5584
                     /locus_tag="PKJ17_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOD74695.1"
                     /translation="MGQQDSGFSAGTVLLSFFIGGILGAGVALLTAPKTGEETRKMIK
                     EFAEDARDRAGEYAGQVKEKASGFVDKGKDFIDKEKNIITKAVEAGKEAYEREKQG"
     gene            5665..>5929
                     /locus_tag="PKJ17_01625"
     CDS             5665..>5929
                     /locus_tag="PKJ17_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74696.1"
                     /translation="MITGNRFYNLVLIFIVAALGYLTYRILDPFLSPIMWAIVFSVVF
                     YPFYAFVLRYVKYRAAASVLTVFAIFLIIIGPFSYFAYLLTQET"
BASE COUNT         1207 a         1752 c         1614 g         1356 t
ORIGIN      
        1 cttcttatca tggacggcct gtgatattcc atggggagta acatacaagg tttcaagcca
       61 aagacattag aatgttcaac tgtctttaag cctgaaacgt gaaatctgga acgtggaacc
      121 atctttaact caccctgaac cggcccatca ggttctgcag cctgtctgag aggccaagga
      181 ggcttgatgc cgaggccgcc gtcatttcag agcttctcga ggtttcgttc gtcacggcgg
      241 ctatgtcgac gatctcgcgg ttgatctcct ccgatgcggc ggacatctgc tccgtcgcgg
      301 aggctatctg catgaccatg gtctggaggc cctcggcact gctcacgatc tcacccaggg
      361 accctcccgt ccgggagaca agctccacgc cggagccgac ctttacgtcg gcctcttcca
      421 tagctctcgt ggcgttctcg acctctgagc gcatcccctc gatcgtctgg ccgatctcca
      481 gggtggcggc cgccgtcttc tcggccagct tcttaacctc ttcggccacg acggcaaacc
      541 ctctgcccac ctctcctgca cgggcggctt cgatcgccgc gttgagagcg agaaggttcg
      601 tctgatcggc tatatcgttg atgagaacga tgatctcgcc tatctgctcg gaccgtcttc
      661 ccagcgactc cacgaactgg cccgaccttc tcaccacctc ggcgatactc ctgacctcct
      721 cgatggattg gctcaccaca tcggctccgc tcttcgcgat gccgacggcc attcccgccg
      781 acgtcgctat gtcattcgtg ttccgtgcca cgtccacgat ggtctgcgac atttcctccg
      841 cggacgaggc cacctgtgaa gatttcgtcg cctgctgtgc cgagccctgc tgcatctggt
      901 ccgcgtgcac gctgagctgc cggctttcgc cgatgatgcg gtccgtcgct tccttgacct
      961 cgtgcacgat ctcccgccag tgaagcacca tgttctgcat ccccttgaga agctggcctg
     1021 tctcactttc gtcctcggcc tttatgtcca cggtcaggtc gccttcggcc agtctttgcg
     1081 cggcgctcac gcactcattg aggggaaggg tcagtttgcg catgtagaag taggacatga
     1141 cgcacaccgc accgatcaat atgatcccga tgatgacgac gaacatgagg atgcggttga
     1201 atgccgcaaa atagtcgccc ttgggcatac ccacgtagag cacgccgatg acctctccgt
     1261 tctggccgcg gatggggtcg taggctacaa agtaggattt ccccagtatg tccgcgctgc
     1321 cgcggaagga cgttttcttc tggaagatgg catcatggac ggggcctttc aggcgcgtcc
     1381 ccaccgcccg cgtgccgtcg ggattgagga cattcgttga gacgcggaca tccctcatga
     1441 agatggtggc cgtcccgccg aagaattcct tgatcttgtc gacgagcaca gtatcgccgt
     1501 tcagaccgta ggctccaacc acgagcctgt cgtcctcgat cgtgaacggg accttttgtt
     1561 tctgcccgga ctgagcggcc tccatggatg actgcgactt cgcattcagg agctcccaga
     1621 acacgttgag tcgtgaatcc atcgtcctgt tcgcctgccg tatcagatca ttccgcatct
     1681 cccacaggca cgctgatgca gttatcacaa ccgccacggc aatgacaccg gagatgaaaa
     1741 gcatgaattt ggtgccgatc ttgagactca ttctcatgga ccaccctctc cccagaatat
     1801 tcaacaacct gctgatctat ccaatgtata tgtctatccc gattatcgaa atttgcggcg
     1861 gtgacttaag cgtgatccct cgacgtcgcg caatgatccc cgcactttcc ctttacaatc
     1921 tccggggccg ctggtacaat cgctaaaatt ccggaggagg tacatcatgc ggtgtgacaa
     1981 ctgtggtaac gaaagccccg agggatcacg gttctgcata aaatgcggaa gggagttcgg
     2041 tgcctccggc tcggggataa cggtctgtcc tcactgcgga gtccagatcg ccccggggtc
     2101 tctgttctgc tccgcctgcg gcaaatccat aggcgccccg cagggggagg tgaaccaggg
     2161 aaggacgtcg cagccccctc tctccgaacc ccccacgcgc cctctcacct cgaacatcgc
     2221 actcgatgtc atcctttccg tcataacctg cgggatctac tggttcttct ggcaggcccg
     2281 ccagatgcgg gccatcaacc acctcctcgg ccaggagaga tacagcttct ggctctggtt
     2341 cttcctgacg ctcatcacat gcggtctcta caacatctac tacgagtact acatggccca
     2401 gggcatcgtg gaggcccagg attcacgcgg ctacttgagg tccaaggacc tgccggtctt
     2461 aagcctcatc cttacgataa tcggcctcaa catcgtgacc gacgcgatac agcagaacga
     2521 gatcaacaag tttttcggca aataagatgg accaccacct tatcccccgg aagatggcct
     2581 cagcctttgc ccttcttctt cttgtcctcg ccgttgtgca cgcgacgggt atcctgccct
     2641 tcggcacgct cgcggggatc cttcccgtct tctgtccctt tgagcttctc acgggcatcc
     2701 cctgccccgg ctgcggcatg acccgcgcca tcctctgcct catcgctggc aacccctccg
     2761 acgccctcct ctacaacccc ttttccttct tcctcctcac cctcgtcacg gcaagcatcc
     2821 tgccgcgccg ctggctggaa aggataccga caggcatccg gtccgtcctg ccgcgcgcct
     2881 acgcggtcat cctcggcctc gtcatcacct tctggatatt cgacaggctg ctgccggcct
     2941 tgaccgccta aaggctgctt aaaccgcaac tcattttctt cgccggaata acgaaagagc
     3001 cccaaatcga cttcagaaat gactttgtca acggcctctt gaacgttaaa ggccaaaaag
     3061 ggtccttcat ccggggtcta tagggacggt atgagggaga attgtctcta aacgttcaag
     3121 ctgttcaagc ggctcgagcg gctgcaactg tccttatcct ggtgcttttt ccggcaggta
     3181 ttcctggcgg atcttggcga ggcttctgaa gatgttcttc ttcacgttgc ggttgtgttt
     3241 cttgagatcg gcgactatgc ctttcaccag gctgcgtttc gatacgtcgc cccggggaca
     3301 ctccgaggcg atgacgggca ggttaagtat tgtggcgaga cgggctagct ccttctcttc
     3361 aacgtaggcg agcgggcgga tgatggcaag ctcaccgtcg aacatctcct ggtatggcat
     3421 catggtaccg atctcgccct ggaagaagag attgaggagc atcgtttcga tgatgtcgtc
     3481 catgtggtgg gcgaaggcga tcttgttggc ccctatgtcc gccgcgacgt cgaagaccgc
     3541 tttgcgtctg ttccagctgc accagaaaca gtcgaggttc tcgatgtccc ccgtccagtc
     3601 ctcgggggcg ctggcgatga tgtaggagag gccttccttt tcgaagtagt cagcgagcat
     3661 gtccgcgttg acccagggga agcccatgtc cacgtgacag gcgatgagtt cgtagttgat
     3721 gggcacgaac ttgagccgct cctgcatgat cttgagaagg cacaggctgt ccttgcctcc
     3781 ggaaacggca atgatgacct tgtccccgtc ggagagcatt ctgtaatccc agatggcctt
     3841 gcccgttttt ttggtgataa agtagaaggg gccgcggatg ttcatgccag cgattcggcg
     3901 agggcccgtg ccttgtctat gtactcttgc ctgacccctc cctccggctc gcagcccggc
     3961 gcgttgacct gtcccgcata tgccaccttg agaaggtcga aggtcttctg gaagatgtcg
     4021 atcgccggac ggcaggaatc catgtccttg tcagcgaagg tcgtcagggt gatggccttt
     4081 ttcccctcga tacgggaacc gtagttctcg tccatgaagg cgatggagcg atccatgacg
     4141 agcttgagct gccccgaggg gccgaaccag tatatggggg tcgcgtaaac gatgaggtcc
     4201 gattccatga tgtacttgcg gacgtccttc atatcgtcat tgatcttgca gacgcccccg
     4261 ggcaggcaga catagcagcc ctggcagggc cggatgttga ggtcgttgag atagagcatc
     4321 cttacgcccc accctctctc cttcaacgtg gccgacaccg cacgggccag ttcgacagta
     4381 ttgcctttct tgcggggact cccgaaaagg ataaccgcat tcttcatcac atcctcctca
     4441 gagacaggta atgggtaatg agccgtgggc gacagggaca cgtgaccaca acctgttttt
     4501 tggttgcctt attatatgga cagacggttt caactttcaa gaaaagaaga ccgtttcagg
     4561 tttcaggttt cgagctaaag acaagaaccg gagaacagcg gccaaaaagg ttctgatcct
     4621 cccggacagt cagactgtga aacctgtctg agcataaggg aactgtacct ccctggctct
     4681 taattcgcaa cgcgcaaccc ggaacccgca actgtctttc aactcaaacg tggaacctga
     4741 aacggttttt tcttcccgaa accttgtctt acggtactac agaggttata atgtattaag
     4801 ttcgttcacg ggatagggca taagctacgt atcctgctcc gcggtcgata tcgacaaaga
     4861 gggggtgcca tgagcaacat ctttctgggt atcataaccc tggcggcagt tgttctcgta
     4921 gtgtacgtcg tctacatgat actaaacctc aagaggacca tagcctcgct tgaaaggttc
     4981 gtggaaacca cggaggcgac cctccagcct gcactggagg aattgcaggc aaccttgaag
     5041 agcgtcagga agatcacaga cgacgtgggc accctgacgg acgatatccg gcacatctcg
     5101 ggctcggtga gaaatgtcgg agaggcggtg gaccaggtga gctacgcggt aaagagcgcg
     5161 gctcaggcgt cggtcagcga aacaaaaggg ttgaaagccg gaatcagggc aggcgtgagt
     5221 tactttctga ggaatgcctg cttgaggggc gggacgcagc aataaaaaaa ggaggtagtg
     5281 tatgggacag caagacagcg gtttcagtgc gggcacggtt cttctctctt tcttcatcgg
     5341 cgggatacta ggcgcgggag tggccctcct cacggcaccg aaaacgggag aagaaacgcg
     5401 gaagatgatc aaggagttcg cggaggacgc gagagacagg gccggggagt atgcgggcca
     5461 ggtcaaggag aaggcatccg gcttcgtcga taagggcaag gattttatcg acaaggagaa
     5521 gaacatcatc accaaggcgg tggaggctgg aaaggaagct tacgaaagag aaaaacaggg
     5581 ctgaaatacc atcgatactc tgatatcatt cactaaggct gtccccgtgg gagggggcgg
     5641 ccttatgctt cttcggtcgg gccaatgatc accgggaaca ggttttacaa tctcgtcctc
     5701 atcttcatcg tcgcggcgct cgggtatctg acatatagga tcctcgaccc ctttctgtcc
     5761 cccatcatgt gggctatcgt cttctccgtc gtcttctacc ccttctacgc gtttgtcctc
     5821 aggtacgtaa aataccgggc ggccgcttcg gtgctgacgg tctttgccat tttcctgatc
     5881 atcatcgggc ctttctccta tttcgcctat ctcctgaccc aggagacgg
//
LOCUS       DAOPOA010000043         5916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_14716_length_5916_cov_3.434567, whole genome shotgun sequence.
ACCESSION   DAOPOA010000043 DAOPOA010000000
VERSION     DAOPOA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14716_length_5916_cov_3.434567"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..2091)
                     /gene="ligA"
                     /locus_tag="PKJ17_01630"
     CDS             complement(88..2091)
                     /gene="ligA"
                     /locus_tag="PKJ17_01630"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOD74697.1"
                     /translation="MDREGIEKRIRHLREELNHHNYRYYVLDDPAVTDREYDDLMREL
                     VRLETEHPEFADPASPTQRVGSRPLDAFSTVKHSIPMLSLQNAMDEEEVRDFHRRVAK
                     LLGTRDVEYVMEVKIDGLAVELVYIDGVFTLGSTRGDGYVGEDVTQNLRTIRAIPLRL
                     GTEGDAPPPERLEVRGEVYMGKKEFLALNGEREQTGEPLFANPRNAAAGSIRQLDSRI
                     TATRKLNIFCYAPGSITGASFSTHMEFLDSLRDWGFRVNDNTRLCRSIEEVVEHYNRV
                     RSMREEIPYEIDGTVIKVNRIDHQTALGTVSRSPRWAIAYKFEAIEAETTVEDILVSV
                     GRTGALTPVASLNPVTVGGVEVSRATLHNEDEIARKDVRVGDRVIVTRAGDVIPEVVR
                     VLDAARPGRTEPFRMPGKCPVCGEAVVRPEGEAIRRCVNISCPAQIKGSIEHFASKRA
                     MDIDGLGTKLVEQLVDRQVIRDVSDLYYIRKETLAGLERMADKSAGNIIDAIEASKHR
                     PFARFLNALGIRQVGEHAADLLAERFDSIEKLMAADTQMLLAIPEIGPEAAGSITAFF
                     RDAKNRETIKRILDAGVKLEYEQGGVKKLAGLTFVFTGSLSSMSRDEARAKVEELGAK
                     TASSVSKKVSYVVAGEEAGSKLDKARQLGLKVLTEREFLELLS"
     gene            2307..>3689
                     /locus_tag="PKJ17_01635"
     CDS             2307..>3689
                     /locus_tag="PKJ17_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD74698.1"
                     /translation="MNRNRTVERSAGRRGRILMLRVFFVALLSLVIALTSAAAGRETG
                     ERSGLAAMPIEELMGVEVSTVLGASRYEQRISDAPASISIITSQDIKRYGYRTMADVL
                     NSVPGLYVTYDRNYHYLGAGGFLRQGDYNTRMLLLVDGHRMNNNIYDTAAVGNDLILD
                     IDLVDRVEVIRGPGSSLYGSNAFFGVINVITKGASHVNGLELSAEGGSSDANKQRATF
                     GRAFGNGLEMVLSATRYHSRGQSLYYKEYDSPATNSGRSDGGDYEGYSSFFTNVKMAG
                     LDVQAAYGSRTKGIPTGAWGTVFNDDRTKSIDAQGYIDVRYHRAFRDNQDLSVRVFYD
                     YYRYDGKYLFDYPPLTINRDLAVGKWWGTEALYKTRIGDRHTVIAGLEYRDNLLQQQK
                     NYDDSPYAVYLDDDRGSYSLGAYAQGEFTVTERILLNLGARYDHYKDFSGRLNPRAAL
                     IWNPLERTTVK"
     assembly_gap    3690..3789
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3790..4510
                     /locus_tag="PKJ17_01640"
     CDS             <3790..4510
                     /locus_tag="PKJ17_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOD74699.1"
                     /translation="LNPRAALIWNPLERTTVKFIYGEAYRIPNAYELYYSDGNVTQGA
                     NPGLDPETIRTYELVLEKYVKEFRLCLSGFYYDVENLISQQTEAAGVMVFANTGRAEG
                     RGIQFEMERKWRNGLAGKLSYSFQEVTSNQDGVEFANFPKHLVKFNAFGPIIRNWLGL
                     GVEIQYASSQRNLNGTRDGGYTLVNATLLSEKTFRDLDLSFSVYNVLDKKYSNPGSRE
                     HVQSDIGQDGRTFRLKATYRF"
     gene            4515..5045
                     /locus_tag="PKJ17_01645"
     CDS             4515..5045
                     /locus_tag="PKJ17_01645"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HOD74700.1"
                     /translation="MKTLLLIAVIWGLALTVQPACLHAQAGQFGEYDVKAVFMANFFK
                     FVEWPAPVREYRICIYGSDPFGQSMRTVEGMKVRGKMLEVRRPASLRALGDCHALFIS
                     SSERRRIHTLLEAVRGMDILTVGDTEGYAQEGVMVNFYIEASKVKFEINLNSVRSSRL
                     TVSSQLLKLGRMVGDE"
     gene            5038..>5916
                     /locus_tag="PKJ17_01650"
     CDS             5038..>5916
                     /locus_tag="PKJ17_01650"
                     /inference="COORDINATES: protein motif:HMM:NF028461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HOD74701.1"
                     /translation="MSRGRALPRVMDLFRNLPLKRKLTLIYLISTIAILGLVGTIITV
                     NEILSARKTFVSDSEGLMEGLAKTSAAAVLFRDQRDAEETLKVLRAFPDVRYAAIMDN
                     VGTIQAEYRRDARRGEGHQGARLGDGYGFSLSEAWFSRVITMDDDRIGSVYLLRDLKR
                     LHGNLYRNIGILAVAVIVSLIIGYYLSLYLQRIVTDPTNRLVTMMRTISDKRQYALRA
                     DAAGRDEIGDLARGFNDMLQTIEERDAELTAHRQHLAELVQKRTEELVRVNGDLTREL
                     EQRERFERALSESEIRY"
BASE COUNT         1115 a         1714 c         1825 g         1162 t
ORIGIN      
        1 cccgtacgtt ccctgccggc cgtcagccca gatccttgtg caggaagtgt tctcgtagac
       61 aggtccggag tatcgggttc ccttccccta gctcaggagt tcgaggaact cccgttccgt
      121 caataccttg aggcctagct gccgggcctt gtcgagcttg gagccggcct cttcgccggc
      181 gacgacgtag ctgaccttct tgctcaccga cgaggcggtc ttcgcgccca gctcctcgac
      241 ctttgcccgg gcctcgtcgc gggacatgga tgagagggag cccgtgaaga cgaaggtgag
      301 gcccgcgagc ttcttcacgc cgccctgttc gtactcgagc ttgacgccgg cgtcgaggat
      361 cctctttatg gtctcgcggt tcttcgcgtc gcggaaaaaa gcggtgatgc tgcccgccgc
      421 ctcggggccg atctcgggta tggcaaggag catctgagtg tccgccgcca tgagcttctc
      481 gatggaatcg aaacgctccg ccagaaggtc cgccgcgtgt tcgcccacct gccggatacc
      541 cagcgcgttg aggaaccgcg caaagggtct gtgtttcgac gcctcgatgg cgtcgatgat
      601 gttcccggca gacttgtcgg ccatccgctc caggccggcg agggtttcct tccttatata
      661 atagaggtcc gacacgtcgc ggatgacctg cctgtcgacg agctgctcca cgagcttcgt
      721 gcccaggccg tcgatgtcca tggcccgctt cgaggcgaag tgctcgatgc tccccttgat
      781 ctgagcgggg cagctgatgt ttacgcagcg ccgtatggcc tcgccctcgg gtctcacgac
      841 ggcctcgccg cagacgggac acttcccggg catcctgaaa ggttccgtcc ggcccggcct
      901 tgcggcgtcg aggaccctga ccacctcggg gatgacgtcg cccgcgcggg tcacgatgac
      961 cctgtctccg accctcacgt cctttcgcgc tatctcgtcc tcgttgtgca gggtggcccg
     1021 ggagacctcc accccgccca cggtgacggg attgagagag gcgacggggg tgagggcacc
     1081 cgtccgcccg acgctgacca ggatatcctc gacggtcgtc tccgcttcga tcgcctcgaa
     1141 cttgtacgcg atcgcccagc ggggcgagcg cgagacggtt ccgagggccg tctgatgatc
     1201 gatcctgttc accttgatga cggtgccgtc tatctcgtag gggatctcct cccgcatgga
     1261 tctgacgcgg ttgtagtgct ccacaacctc ttcgatgctg cggcagagcc gggtgttgtc
     1321 gttgaccctg aagccccagt ctctaaggct gtcgaggaat tccatgtggg tggagaacga
     1381 ggccccggtg atgctgcccg gggcgtagca gaatatgttg agctttctcg tcgcggtgat
     1441 cctggagtcg agctggcgga tggaacccgc cgccgcgttt cgcgggttgg cgaagagagg
     1501 ttcccccgtc tgctcccgct cgccattgag ggcaaggaac tcctttttcc ccatgtagac
     1561 ctcaccgcgg acctcgaggc gctcgggcgg gggcgcgtcc ccctcggtcc ccaggcgaag
     1621 cgggatggcc ctgatcgtcc gcaggttttg cgtcacatcc tcccccacgt acccgtcacc
     1681 ccgcgtggag ccgagcgtga agacgccgtc gatgtagacg agttccacgg ccagcccgtc
     1741 tatcttgacc tccatgacgt attcgacgtc tctcgtcccg aggagcttcg cgacgcgcct
     1801 gtgaaagtcc ctgacctcct cctcgtccat ggcgttctgg aggctcagca tcgggatgga
     1861 gtgcttgacg gtcgagaagg catcgagggg ccgcgaccca acccgctgcg tcggtgacgc
     1921 ggggtcggcg aattccggat gctccgtctc aaggcggaca agctcgcgca tgaggtcgtc
     1981 gtattccctg tccgtcaccg cggggtcgtc gaggacgtag tagcggtagt tgtggtggtt
     2041 gagctcctcc ctgagatgcc ggatcctttt ttcgatacct tccctgtcca tattctttcc
     2101 gttgtgtccg ggacccctcc ggggttcgtc cgggtgatat gtgtatcata tcgcaggtgt
     2161 tctttttgag tccacacatt tgggacgacg ctgtcttcgg cagttttgga ggtgttttat
     2221 cccttggaat atgttaaacg atttcaaaga cgtgccgata tatcaggtat ccggtgaccg
     2281 ttgacggcgc cgaggaaggg acatctatga accgcaacag gactgtcgag aggtccgcag
     2341 gtcgacgggg caggattctg atgctccggg tcttcttcgt tgctttgctt tccctcgtca
     2401 tcgcgcttac ctccgctgcg gccgggcggg agaccgggga gaggtcaggg cttgccgcga
     2461 tgcccatcga agagctgatg ggcgtggagg tgtcgacggt gctcggcgcg tcgcggtacg
     2521 agcagcggat atccgatgcc ccggcctcca tcagcatcat cacctcacag gacataaagc
     2581 gttacggata ccggaccatg gccgatgtcc tcaacagcgt gccggggctg tacgtcacgt
     2641 acgaccggaa ttaccattac ctgggcgccg gcggtttcct gaggcagggg gattacaaca
     2701 cgcgcatgct gctccttgtc gacggccaca ggatgaacaa caatatttac gatactgcgg
     2761 cggtggggaa cgatctcatc ctcgacatcg acctcgtgga ccgtgtcgag gtgatacggg
     2821 ggccggggtc ctcactgtac gggagcaatg cctttttcgg cgtgatcaac gtgataacga
     2881 agggcgcaag ccacgtgaac ggcctggaac tgtcggccga agggggcagc tccgacgcga
     2941 acaagcagag ggccaccttc ggccgcgcct tcggcaacgg actggagatg gtgctgtcgg
     3001 cgacccgcta ccacagccgg ggacagagcc tgtactacaa ggaatacgac agccccgcga
     3061 caaattcggg acggtccgac ggcggcgact acgagggata ctcatccttc tttacaaacg
     3121 tcaagatggc cgggctcgat gttcaggcgg cctatggttc ccgcaccaag ggcattccca
     3181 cgggcgcctg gggaaccgtt ttcaatgatg acaggacgaa gtccatcgat gcccagggct
     3241 acatagacgt caggtatcac agggcgttca gggacaacca ggacctctcc gtgcgggtct
     3301 tctacgatta ttaccgctat gacgggaaat acctcttcga ctatccgccc cttaccatca
     3361 acagggacct tgcggtcggc aagtggtggg ggacggaggc gctctacaag acccggatag
     3421 gggaccggca taccgtgata gcgggattgg aataccggga caaccttctc cagcagcaga
     3481 agaactatga cgacagtccc tacgcggtct acctcgacga cgacagggga tcgtacagcc
     3541 tgggtgccta cgctcaggga gagttcacgg tcaccgaaag gatactgctc aatctgggcg
     3601 ccagatacga ccactataag gatttctcgg ggcggttgaa ccccagggcc gcgctgatat
     3661 ggaaccccct ggaaagaacg acggtgaagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnng ttgaacccca gggccgcgct gatatggaac cccctggaaa gaacgacggt
     3841 gaagttcatc tatggtgagg cctaccgcat acccaatgcg tatgagctgt attattcgga
     3901 cgggaacgtg acgcaagggg caaatcccgg cctcgatccc gagacgatca ggacctacga
     3961 gctcgttctc gagaagtatg tgaaagagtt ccgcctctgt ctctccggtt tctactacga
     4021 tgtggagaac ctcataagcc agcagaccga ggccgccggg gtcatggtct tcgccaacac
     4081 gggaagggcc gagggcaggg gcatccagtt cgagatggag aggaaatggc ggaacggtct
     4141 tgcgggaaag ctctcctact cctttcagga ggtcacgagc aaccaggacg gagtggagtt
     4201 cgcgaatttt ccgaagcatc tggtgaagtt caacgccttc ggtccgataa tccggaactg
     4261 gctgggcctg ggagtggaga tccagtatgc gagctcgcag aggaacctta acggcacccg
     4321 cgacgggggc tataccctgg tcaatgccac gctcctgagc gagaagacct tcagggacct
     4381 tgacctttct ttcagcgttt acaacgttct cgacaagaag tacagcaatc cgggttcgcg
     4441 cgagcatgtc cagagcgaca tcggacagga tggcagaacc ttcaggttaa aggcgacata
     4501 tcggttctag gaagatgaag accctgcttc tcatagcggt catctgggga ctcgcactca
     4561 ccgtccagcc tgcctgtctt cacgcgcagg cggggcagtt cggggaatat gacgtgaagg
     4621 ccgtgttcat ggcgaatttc ttcaagttcg tcgagtggcc cgctccggtc cgggaataca
     4681 ggatctgcat ttacggttcg gaccccttcg ggcagagcat gcggaccgtg gagggaatga
     4741 aggtgcgcgg aaaaatgctg gaggtgagaa ggccggcctc tctgagggcc ctcggggact
     4801 gccacgcgct cttcatctcc tcctcggaga ggcggcgcat ccataccctc cttgaggccg
     4861 tcaggggcat ggatatcctg accgttggag acaccgaggg ctacgcgcag gagggggtca
     4921 tggtgaattt ctacatagag gcgagcaagg tgaaattcga gatcaatctc aactcggtgc
     4981 ggagctcccg tctcaccgtc agctcccaac tcctgaagct gggaaggatg gtgggcgatg
     5041 agtaggggca gggcgttgcc gcgggtgatg gaccttttcc ggaatctgcc cctcaagcgc
     5101 aagctcaccc ttatttacct catttccacg atagccatcc tgggtctcgt cggaacgatc
     5161 atcaccgtca acgagatcct gtccgcgcgg aagaccttcg tatctgattc ggaagggctc
     5221 atggagggcc tggcgaagac cagcgcggcg gctgtgctct tccgcgatca gagggacgcg
     5281 gaagagacgc tgaaagttct cagggccttt ccggatgtcc ggtacgccgc gatcatggac
     5341 aacgtcggga ccatacaggc cgaatacagg cgcgacgccc gccgcggcga aggtcatcag
     5401 ggtgcgaggc tcggggacgg ttacggtttc agtctctccg aggcgtggtt ctcccgggtc
     5461 ataaccatgg atgatgaccg gataggttct gtctatcttc tccgcgacct gaagaggctt
     5521 cacgggaacc tgtatcggaa catcgggatc ctggccgttg ccgtcatcgt gtccctcatc
     5581 atcggttact acctttccct ctacctccag cggatcgtca ccgaccccac gaacaggctt
     5641 gtcacgatga tgaggaccat cagcgacaag cggcaatacg cgttgagggc ggacgcggcg
     5701 ggaagggacg agatcggtga cctcgcgagg ggtttcaacg atatgcttca gacgatagag
     5761 gagagggacg cggagctgac cgcgcaccgc cagcacctgg cggagctggt gcaaaagcgc
     5821 accgaggagc tcgtcagggt gaacggtgat ctgacgcgcg agctggagca acgcgaaagg
     5881 ttcgagcgtg ccctcagcga atcggaaata cgctac
//
LOCUS       DAOPOA010000044         5843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_14977_length_5843_cov_3.115930, whole genome shotgun sequence.
ACCESSION   DAOPOA010000044 DAOPOA010000000
VERSION     DAOPOA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14977_length_5843_cov_3.115930"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKJ17_01655"
     CDS             complement(<1..245)
                     /locus_tag="PKJ17_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74702.1"
                     /translation="MEKESKELMRSESGRMMHPLWDMERWLERNFMRPLSLFHPMMTR
                     SLTEELMPPVDILEENSEVVIRMEVPGVKKEDIDVTLT"
     gene            542..2314
                     /locus_tag="PKJ17_01660"
     CDS             542..2314
                     /locus_tag="PKJ17_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOD74703.1"
                     /translation="MGTERSYHLSSRIQLIGILFLLFVVDGLALWFTARFDYTRTLEH
                     AKTILQKTTISLEERVRRTIDSTEAILHGRVARLQEKGPKATVSSREEWERLRKAAEM
                     LPDPGSLWILDDKADLLMDSTEYPSQKMNYSEREYFAAHRDQGIQLYVGPVVKGKVTK
                     KYSFTISYRIGGKDGRFLGVVLAAIDVDDFTGFLRTIGVGEDGAVAVFRTDGTLIFRQ
                     PMKEEYLGRSFKKLKLFSMPFDLSPSGEYESDMWVDGVRRLVAYRKIHGRPLVVVTGV
                     PTGSVLKEWQTRFRFYVSIAVIIFFALAGLSWFAHRTVSREERERSREIAGINRSLTT
                     EIAERKKVEEELQNARDELESRVRQRTREVNAAYEQLRKVSSHVESIREEERARISRE
                     IHDELGQALTGIKMELSWLITKPPEDRQSLADKVKPMLELTDKTIRTVRRISTELRPG
                     ILDDLGLAAAIEWLVEDFKNRTQIECRLLSGLKEAVPDPSLSTALFRILQETLTNVIR
                     HAEATSVTINMREDSGVIVLEVEDDGKGITEHDISDTRSLGIIGIRERVLLLGGRLRI
                     SGSTGKGTVIVATIPLKRPDENHD"
     gene            2307..2933
                     /locus_tag="PKJ17_01665"
     CDS             2307..2933
                     /locus_tag="PKJ17_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD74704.1"
                     /translation="MIKIIIADDHPVVRKGLKQILSEETDVELVKEARNDSEVLNLLH
                     AHDWDAVVLDITMPGRGGLELLTELKRLRPKLPVLVLTVHPEDQYAVRALKEGASGYM
                     NKESAPEELVQAIRKIIRGGKYVSAALAEKLASMIGAEAQPHENLSIREYQVMLLIAS
                     GKTVSEIAEEMSLSIKTISTYRTRILEKMGMTNNVELARYAMKHNLIQ"
     gene            3078..3458
                     /locus_tag="PKJ17_01670"
     CDS             3078..3458
                     /locus_tag="PKJ17_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD74705.1"
                     /translation="MRLFIADDSEVVRERLSTLLSQLDNVEIVGNAGTVDEAIHEIRR
                     LKPDLVVLDIRMPGGCGIDVLRSVKGDEPSPAVIMLTNYPYRQYEERCMDAGADFFFD
                     KSVEFEFAIEVIKGLASAQIQKRE"
     gene            3479..4114
                     /locus_tag="PKJ17_01675"
     CDS             3479..4114
                     /locus_tag="PKJ17_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD74706.1"
                     /translation="MDVIALAKHEFPFKSIPPFAEKRGTRQREMDLQQIIRNIQMLLP
                     QELQRNIELTLAEQELPVMIDMAQLQAALLNLIRNAQEAMPEEGRITISTGSARLYGD
                     SAGKRGRGSPKTWAFVSISDTGSGIDEETRSRIFEPFYTTKGDPHQGLGLPMASRTIR
                     QHNGTIDIETRSGHGTTVKVYLPFAGRKPARRKAIPLPPAGTAIVAPLRQR"
     gene            4137..4586
                     /locus_tag="PKJ17_01680"
     CDS             4137..4586
                     /locus_tag="PKJ17_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD74707.1"
                     /translation="MFAPKNILVPTDFSEFSDNALEKAYDIAKQYKAKIHLLHVIGVV
                     QTCAVDYCFDQATIDDLDRKAAESSKEIMRKQIEKVVKSKDVEIAAYVTKGTPYEEIL
                     KEQQSKNIDLIVIASHGRTGILGHLIGSVAEKVVRAATCPVVLVKNR"
     gene            4701..5282
                     /locus_tag="PKJ17_01685"
     CDS             4701..5282
                     /locus_tag="PKJ17_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOD74708.1"
                     /translation="MLPIGPLMIEHRLIEKMIGLIQRRADRYAREGDIDPVFIDSVID
                     FMRTYADRCHHGKEEEILFRELNKKVLPPELKHILDQLVEEHRQGRQTVADLVEARQE
                     YARGDREAVRTILDRLRYMTDLYPRHIEKEDGHFFIPCMSLFTQEENEEMLNEENAFD
                     RELVHQIYREKISAWGKESDGEEDTRDTREKGL"
     gene            5257..5622
                     /locus_tag="PKJ17_01690"
     CDS             5257..5622
                     /locus_tag="PKJ17_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOD74709.1"
                     /translation="MIRVKRVYDAPAGDDGYRVLVDRLWPRGMSWERSRIDLWLKDIA
                     PSNQLRRWFHHEPEKWAEFKQRYFAEIRDRTDVLGSLFEKLKTERVTLLYGARDRQFN
                     NATALKEYLENKPAADDKV"
     gene            5644..>5843
                     /locus_tag="PKJ17_01695"
     CDS             5644..>5843
                     /locus_tag="PKJ17_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HOD74710.1"
                     /translation="MDWIPAILAGLPIAIVGSLMVFLSWPAKWAMPVGWASAALIAGL
                     WWNMPVRWVAASTIAGFINALD"
BASE COUNT         1464 a         1554 c         1716 g         1109 t
ORIGIN      
        1 gtgagcgtaa catcgatatc ctccttcttc acaccgggca cctccatcct gatcacaacc
       61 tcgctgttct cctccagtat gtccacggga ggcatcaact cctccgtcag tgatctggtc
      121 atcatcgggt gaaagaggga caagggccgc atgaaattcc tttccagcca tctttccatg
      181 tcccagagag gatgcatcat tctgccggat tcggacctca tcaattcctt tgactccttt
      241 tccatggcac acctccattt ttgcgtaata agcgtttcta cccggtaata ccaaggttgt
      301 tggtctacaa taacaggata atcagggaag ctcctccggt ctgtaggaga aaggacaatt
      361 cttcgacggt ttttcgacaa cggacatgtc atccctttca atgacacgcc tggaaggccg
      421 cctgtcaggc gatgagcgcc tccggcacca tcagaggttg gcatatcgag caaggcatga
      481 taatcagtat agaaggaagc tatcaccggc cggtcattac caaaccccaa ggggctccag
      541 ggtgggaaca gaacgatcat atcatctttc ttccaggatc cagctgatcg gcatcctttt
      601 tcttctcttc gtcgtcgacg gccttgccct ctggttcacg gcgagattcg actacacgag
      661 gaccctcgag cacgcgaaaa ccatccttca aaagaccact atctcattgg aggaaagggt
      721 gagaaggacg atcgactcaa cggaggcgat cctgcatggc agggttgcca ggcttcagga
      781 gaaggggccg aaggcaaccg tctcgtcgcg ggaggagtgg gagcgcctca gaaaggccgc
      841 ggagatgctt cccgatcccg ggtcgctctg gatcctcgac gataaagccg atcttctcat
      901 ggactcgaca gagtaccctt cacagaagat gaattactcg gaacgcgagt atttcgcggc
      961 tcacagggat caggggatac aactctacgt cgggcccgtc gtcaagggca aggtcacaaa
     1021 aaaatacagt ttcaccatca gttacaggat cggcggaaaa gacggcaggt tcctgggtgt
     1081 cgtcctggca gccatagacg tcgatgactt tacgggtttc cttcgcacga taggtgtggg
     1141 cgaagacggc gccgtggccg tcttccgcac ggacgggacc ctgatcttcc ggcagccgat
     1201 gaaggaggaa tacctcggaa gatcattcaa gaagctcaag ctattcagca tgccttttga
     1261 cctgtccccc tcaggtgaat atgagtccga catgtgggtc gatggcgtaa ggcgccttgt
     1321 cgcctatcga aagatccacg ggagaccgct ggtcgtggtg accggcgttc cgaccggctc
     1381 agtcctgaag gagtggcaaa cccgcttcag gttctacgta tccatagccg tcatcatctt
     1441 cttcgccctg gcgggcttgt cgtggttcgc ccacagaaca gtgtcacgtg aggagaggga
     1501 aaggtcgaga gagatcgccg ggatcaaccg ctccctcacg acagagatcg ccgagaggaa
     1561 gaaggtggag gaagagttgc agaacgcccg cgacgaactg gagtctcgag tgcggcagag
     1621 gacccgtgag gtgaacgcgg cgtacgagca gttgagaaaa gtttcctcgc atgtcgagtc
     1681 catcagagag gaggaaaggg cgaggatctc gcgcgagatc catgatgagc ttggccaggc
     1741 gttgacggga atcaaaatgg agttgtcgtg gttgatcacg aaacctcccg aagaccggca
     1801 gtcgctggcc gacaaggtca aacccatgct ggaactgacg gataagacga tacggacggt
     1861 gcgaaggatc tcgacggaac tgcggccggg gatactcgat gacctggggc tggccgcagc
     1921 catcgagtgg ctcgtggagg atttcaaaaa ccgcactcag atagagtgcc gtctcctctc
     1981 gggtctcaag gaagccgtgc ccgacccctc gctttccacg gccttgttcc gtattctcca
     2041 ggaaacattg acgaatgtaa ttcgacacgc cgaggcgaca agtgttacaa ttaatatgag
     2101 agaagattcg ggcgtgatag tcctcgaggt tgaggatgac ggcaaaggga tcaccgagca
     2161 cgatatctcc gatacccggt cgctcgggat catcgggatc cgcgagcgtg tattgcttct
     2221 cggagggcga ctgcgtatca gcggcagcac cgggaaagga acggtgatcg tggcaacaat
     2281 cccgttgaag cggcctgacg aaaatcatga ttaagatcat catagccgac gaccatcctg
     2341 tagtgcggaa aggactgaaa cagatcctct cggaggagac ggacgtcgag ttggtgaaag
     2401 aagcacggaa cgacagcgaa gtgctgaatc ttctccatgc ccacgactgg gatgcggttg
     2461 tcctcgacat caccatgccg ggtcggggtg gactggagtt gctcacggaa ttgaagcgtc
     2521 tgcgcccaaa acttcccgtt ctcgtcctga ccgttcaccc tgaggaccaa tacgccgtga
     2581 gagccctcaa ggagggagcc tccggttaca tgaacaagga gagcgccccc gaggaactcg
     2641 tacaggccat ccggaagata atcaggggag gaaagtacgt gagtgcggcc ctcgcggaaa
     2701 aactcgcgtc catgatcggc gccgaagccc agcctcacga gaacctgtcg atacgcgaat
     2761 accaggtaat gctgctgatc gcctcgggca agaccgtgtc agagatcgcg gaagagatgt
     2821 cgctcagcat aaagacgatc agcacataca gaacacgtat tctcgaaaag atgggaatga
     2881 caaacaatgt tgaactggca cgctacgcca tgaagcacaa tctcatccag taacaccgcg
     2941 cccgcgtccg tataccggga aaccgctttc ctacgagagc acatatggaa ttcctacaat
     3001 acaattgggc gcaatccgat tttcttcacg gcgacatcag ggtatcctca atagagaaag
     3061 atcagggaag agggacaatg agacttttca tcgccgacga ttccgaggtg gtgcgcgaaa
     3121 gactgtccac gttgctttct cagcttgaca atgtggagat cgtcgggaac gcaggcacgg
     3181 tagacgaagc catacacgag atacggagac tgaagcccga tcttgtcgtg ctggacatca
     3241 gaatgccggg gggctgtgga atcgacgtgc tgaggagcgt aaagggcgat gaaccatctc
     3301 ccgcggtgat catgctcacc aattacccgt accggcaata cgaggaacgg tgtatggatg
     3361 cgggggctga tttcttcttc gacaagtcag tggaattcga attcgcgatt gaggtcataa
     3421 aaggtctggc gtcggcgcag atccagaaaa gagaatgaat tttaaacctt caggaggtgt
     3481 ggacgtgata gcactcgcca aacatgagtt tcccttcaag agcatccccc catttgccga
     3541 aaagaggggg acgaggcaga gagaaatgga cctgcaacag attatacgca acatacagat
     3601 gctcctgccg caggagctcc aaagaaatat cgagttgaca cttgcggagc aggaattgcc
     3661 tgtgatgata gacatggcgc agctccaggc cgcccttctg aatctcatca ggaacgccca
     3721 ggaggccatg ccggaagaag gacgcattac gatcagcacg ggttccgcca ggctctacgg
     3781 tgattccgcc gggaagagag gccgcggcag ccccaaaacc tgggccttcg tctccatctc
     3841 ggataccggc tccgggatcg acgaagagac acgatccaga atatttgaac ctttctatac
     3901 gacaaaggga gacccgcacc agggactggg cctccccatg gcctcacgca ccatccggca
     3961 gcacaacggg accatcgata tcgagaccag atccggacac ggaaccacgg tgaaggtcta
     4021 tctcccgttc gccggaagga agcccgcgcg ccgcaaggcg atacctcttc ccccggcggg
     4081 aacagccatc gtcgcgccgc tgcggcagcg gtgaataaac ctttaaggag gcatctatgt
     4141 tcgcaccaaa gaatatcctg gtacccacgg atttctccga gttctccgat aatgccctgg
     4201 aaaaggccta cgacatcgcg aaacagtaca aggcgaagat ccatctcctc catgtcatcg
     4261 gcgtcgtcca gacctgcgcc gtcgattatt gtttcgatca ggcgaccatc gatgacctgg
     4321 acaggaaggc ggccgaatcc tcgaaggaga tcatgcggaa gcagatcgag aaggtcgtca
     4381 agtcgaagga cgtggagatc gcggcctatg tgacgaaagg aacaccttac gaggagatat
     4441 tgaaggaaca acagtccaaa aacatcgacc tcatcgtgat agcgtctcat ggcaggacgg
     4501 ggatactggg ccatctcatt ggaagcgtgg cggaaaaggt ggtgcgggca gccacgtgcc
     4561 cggtggtcct ggtcaagaac aggtaagggg cgacaaagga cgcgcggccc cggaacgagg
     4621 ctctgcgctt cgccccgata tcgcggaggt taccggaagg cgatgcggag cggacgacat
     4681 caaacaggag gaggtctccg atgctgccga tcggtccctt gatgatcgaa cacagactga
     4741 tagagaagat gataggtttg atccaaagaa gagctgacag gtacgcgcgg gaaggtgaca
     4801 tcgaccccgt attcatcgac tccgtcatcg acttcatgcg cacctatgcc gacaggtgtc
     4861 accacggaaa agaagaagag atcctcttca gggaactgaa caagaaagtg cttcccccgg
     4921 agctgaaaca catcctggac cagctggtcg aggaacaccg gcaggggcgc caaacggttg
     4981 cggacctggt cgaagcgaga caggaatacg cgcgtggcga tagagaggct gtacgaacga
     5041 tcctcgatcg cctgcgttat atgaccgatc tttacccgag gcacatagaa aaggaggacg
     5101 ggcatttctt cattccctgc atgtcgcttt tcacccagga agagaacgaa gagatgctca
     5161 acgaggaaaa cgcgttcgac agggagctgg tccatcagat atacagggaa aagatcagcg
     5221 cctggggcaa ggaatccgac ggtgaggaag atacccgtga tacgcgtgaa aagggtctat
     5281 gacgcccccg cgggggatga cggatacagg gtcctggtgg acagactctg gccgcggggc
     5341 atgagctggg aaaggtcccg catcgatctg tggctgaagg acatcgcgcc aagcaaccag
     5401 ctgcggagat ggtttcatca cgaaccggag aagtgggccg agttcaaaca gaggtacttt
     5461 gccgagatca gggacaggac cgatgtgctg gggtcgctgt tcgaaaagct caagacggag
     5521 cgtgtgaccc ttctctatgg cgcgagagac cggcagttca acaacgcgac agcgctgaaa
     5581 gagtaccttg agaacaagcc ggcagcagat gacaaagtat gagcgaggga ggtcgggtgc
     5641 cgcatggact ggataccggc tattcttgcg ggccttccca tagccatagt gggttccctt
     5701 atggttttcc tttcctggcc cgccaaatgg gcgatgccgg tcggttgggc ctcggccgcg
     5761 ttgatcgccg gtctttggtg gaacatgccg gtccgatggg tcgccgcctc aacgatcgcc
     5821 gggtttatca acgccctcga tat
//
LOCUS       DAOPOA010000045         5831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_15014_length_5831_cov_3.583795, whole genome shotgun sequence.
ACCESSION   DAOPOA010000045 DAOPOA010000000
VERSION     DAOPOA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15014_length_5831_cov_3.583795"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /gene="alr"
                     /locus_tag="PKJ17_01700"
     CDS             <1..1128
                     /gene="alr"
                     /locus_tag="PKJ17_01700"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOD74711.1"
                     /translation="SMSSSPRAVAYIDLGALEENCRSIRRCLEPGTALLGVVKADAYG
                     HGALEVSKRLESVGIDYLGVATVDEGVDLRKGGIATPLLIMSGVFSWEEIEPVVRYDL
                     TPVIYDDALLGRFVALSREFARPVRVHVKFDTGMGRLGFKPAEAQRVAAALADAPGIR
                     TEGIMTHFPSPEVRDEYGLSQVRTFTKVVDTFRTAGLDPAYVHMSSSGAIVTYPEARF
                     SLVRAGISLYGSHPSRSLEEALPLRQVMKVVARIAFCREFPAGVPLSYGRTYQTPRDT
                     RIAYIPVGYSDGYPRSLSNKGRVLIKNRECNIVGRVCMDWTLADVTGVDGVESGDEAI
                     LLGEGDSRGTRVSANEMAELAGTIPYEILCKISRRIKRVYI"
     gene            complement(1266..2504)
                     /locus_tag="PKJ17_01705"
     CDS             complement(1266..2504)
                     /locus_tag="PKJ17_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOD74712.1"
                     /translation="MIDERKKFQEILNVGLEVIQTRDIDILLERILTKARYLTNADAG
                     SIYIKDGDTLLFRYTQNETLQKQLPAGRKLIYSTFTIPVNNQSIAGYVATTGQVLNLE
                     NVYHLHGSVPFSFDPSYDKLSGYRTQSVLSFPLKTHNEEVVGVLQLINATNQDGHIVP
                     FQSDDEPFVVHFANNAAIAVERATMTRDIILRMIKMAELRDPMETGAHVNRVSSYSVE
                     LYEAWAASKGIGKAEVEQNKDILKMSAMLHDVGKVAITDLILKKPARLDSYEYEVMKQ
                     HTYLGAQLFSHQRSDFDKASFLVALNHHERWDGKGYPGYIDFKTGEPLRGFEDLSGRA
                     IGKKEEEIPIFGRIVAIADVFDALSSGRSYKEPWEEERVLETMDRERGKHFDPEMLDV
                     FFSILDVIRNIMQRYPDNNE"
     gene            complement(2550..3800)
                     /gene="pfp"
                     /locus_tag="PKJ17_01710"
     CDS             complement(2550..3800)
                     /gene="pfp"
                     /locus_tag="PKJ17_01710"
                     /inference="COORDINATES: protein motif:HMM:NF041103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOD74713.1"
                     /translation="MNAETIGIIVGGGPAPGINGVISAATIEAINSGKKVVGIKGGFK
                     ALFEGHKDIFMPLTIDDVSRIHASGGSILRTSRDKPENAKAKFKTLMATLKSAGIKYL
                     ITIGGDGTLYMANWIEREARGTISVVHTPKTIDNDIPLPGGFSTFGFQTARHVGVYIV
                     NNIIEDARTMGRWYFITTMGRHAGHLALGMGKAAGATLSLIPEEFPEEKLSFKKVADI
                     LTGSIVKRKSMDRDYGVAILAEGISEKFDLEELGKYEEVERNEKGELKLSEIQLGRVL
                     REFVNRTLGEMGIEVNIVDKNIGYELRAANPIPFDVEYTRNLGYGAVRYLLKGGTGSM
                     IVAYEGNLRPLPFVEIIDYRTGKVKIRTVDMATETYEVARKYMIRLEREDFEASHLAH
                     LARTANMPPEDFKARFEYVLGNYG"
     gene            3876..4709
                     /locus_tag="PKJ17_01715"
     CDS             3876..4709
                     /locus_tag="PKJ17_01715"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOD74714.1"
                     /translation="MKIGFHVSIAKGFDDTLKGAKALGCEVAQVFVKNPRSWEQRVLS
                     DADLAAFGRLSAELPVFAHLSYLPNLAKIDGDERNLKGLLHEAALCEKLGIGSLVVHP
                     GSRPDRLSGARMTAEAIDRVHDRHDVTVLIENTAGQGNVIGRDVEELARMYEGVSNKE
                     KTLFCIDTAHLFESGWDIRKKGVWKGFLDEFERTLGLDRIGLFHLNDSKAAAGSHADR
                     HWHIGQGEIGLTFFRMLVRDKRFAHLGGVMETPKMGNMDEVNMEVMRSLLPPLVPRSS
                     S"
     gene            complement(4674..4766)
                     /locus_tag="PKJ17_01720"
     CDS             complement(4674..4766)
                     /locus_tag="PKJ17_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOD74715.1"
                     /translation="MGSVMKWRKKKIRKHKYKKLRRRTRHQRRK"
     gene            4953..5026
                     /locus_tag="PKJ17_01725"
     tRNA            4953..5026
                     /locus_tag="PKJ17_01725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4987..4989,aa:Arg,seq:tct)
BASE COUNT         1159 a         1607 c         1720 g         1345 t
ORIGIN      
        1 tcaatgtcga gctctcccag ggcggtcgcc tacatcgacc ttggcgccct ggaggaaaat
       61 tgccggtcca tcaggcggtg ccttgagccc ggcaccgcgc ttctcggtgt cgtgaaggcg
      121 gatgcctacg gccacggggc ccttgaggta tcgaagaggc tggaatccgt cggcatcgac
      181 tacctggggg tggcaacggt cgacgaaggc gtggacctca ggaagggcgg gattgcgacg
      241 ccccttctga tcatgagcgg cgtcttctcc tgggaggaga tcgagcctgt cgtccggtac
      301 gatctcacgc ccgtcatcta cgatgacgcc ctcctcggaa ggtttgtcgc cctctccagg
      361 gagttcgcgc ggcccgtcag ggtgcacgtg aagttcgata cgggcatggg acggctcggc
      421 ttcaaacctg ccgaggcaca gagggtcgcc gccgctcttg cggacgcgcc ggggattaga
      481 acggaaggca tcatgacgca ctttccctcg ccggaggtac gtgacgagta cggactttcc
      541 caggtgagga ccttcacgaa ggttgtggat accttcagga cggcaggtct cgacccggcg
      601 tacgtgcaca tgagctcgag cggcgccatc gtgacctatc ccgaggcccg gttctccctc
      661 gtccgggcgg ggataagcct ctatggttcc cacccgtcgc gctccctcga ggaggccctc
      721 cctttgagac aggtcatgaa ggtggtggcc cgcatcgcgt tctgcaggga gtttcccgca
      781 ggggttccgt tgagctacgg aaggacctac cagacacccc gggacacgcg gatagcatac
      841 atacccgtgg gatactccga tggatacccc cggtccctgt cgaacaaggg ccgtgtcctc
      901 atcaagaaca gggaatgcaa catcgtcggc agggtgtgca tggactggac cctcgccgac
      961 gtgacgggcg tcgacggcgt cgagagcggc gacgaggcga tcctcctcgg cgaaggcgac
     1021 agccgcggca cacgcgtttc cgccaacgaa atggccgaac tggccggaac catcccctac
     1081 gaaatactct gcaagatatc gagaaggatc aaacgggtgt acatctaaag acggcttgaa
     1141 tcgcttgagt ggcttgagag ttaaaggcca aaagaatacc ttgggttatg cgtggccccg
     1201 gacaagccac agaagcaatc ttcttgtctt tcaccttcaa gcgattcaag ctattcaagc
     1261 cgcctctact cgttgttatc cggataccgt tgcatgatgt tgcggatgac gtcgaggatg
     1321 gagaagaaga cgtcgagcat ttcggggtcg aagtgtttgc cccgttcacg gtccatcgtt
     1381 tcgaggacgc gctcctcctc ccagggttcc ttgtaggacc ttcccgagct cagggcgtcg
     1441 aagacgtcgg cgatggcgac gatgcggccg aaaatgggga tctcctcctc ttttttgccg
     1501 atggccctgc cgcttaagtc ctcgaaccct cgcaggggct cgcctgtctt gaagtcgata
     1561 tagccgggat agcccttgcc gtcccacctt tcgtggtggt tgagggcgac aaggaaggag
     1621 gccttgtcga aatcggagcg ctggtgggag aagagctgcg ccccgaggta tgtgtgctgt
     1681 ttcatcacct cgtactcgta gctgtcgagg cgggccggtt tcttgaggat gaggtctgtt
     1741 atggccactt ttcccacgtc gtggagcatg gcggacatct tgaggatatc cttgttctgt
     1801 tccacctctg cctttccgat gcctttactt gccgcccatg cctcgtagag ctcgacggaa
     1861 taggatgaaa cgcggttgac gtgggcgccc gtctccatcg ggtcccggag ctccgccatc
     1921 ttgatcatgc gcaggatgat gtcgcgtgtc atcgtcgccc gttcgacggc gatggcggca
     1981 ttgttggcaa agtggacgac gaagggctcg tcatcggatt gaaatgggac gatatgccca
     2041 tcctggttcg tggcgttgat gagctggagc accccgacca cctcttcgtt gtgggtcttc
     2101 agggggaagg acagcacgga ctgcgtcctg tacccggaga gcttgtcgta ggaagggtcg
     2161 aatgaaaagg gtacgctccc gtggagatga taaacattct ccaggttgag gacctgtccc
     2221 gtggtggcga catagcctgc tatggactga ttgttgacgg gaatggtgaa ggttgaatag
     2281 atgagcttcc tgccggccgg gagttgtttt tgcagtgttt cgttctgagt gtagcggaac
     2341 agaagggtat cgccgtcttt gatatagatg gaccccgcgt cggcgttcgt caggtaccgg
     2401 gccttcgtga gaatgcgctc caggaggata tcgatgtccc ttgtctggat cacttcaagg
     2461 ccgacattga ggatttcctg gaatttcttc cgctcgtcta tcatgtcatg tgtgcgggat
     2521 ctcgcgaata cgtgccggaa gaatcgcgtc tagccatagt tgccgagcac gtactcgaag
     2581 cgcgccttga agtcttccgg cggcatgttg gcggtccggg cgaggtgagc cagatgggaa
     2641 gcctcgaagt cctccctctc aaggcgtatc atgtatttcc tggcgacctc atatgtttct
     2701 gtcgccatat ccaccgttct gatcttgacc tttcccgttc tgtagtcgat gatctcaaca
     2761 aagggaaggg gcctcagatt gccttcgtag gctactatca ttgagccggt gccgcccttg
     2821 agaaggtacc tgacggcgcc gtagccgagg tttcgcgtgt attcgacatc gaaaggtatc
     2881 ggattggcgg cgcgaagctc gtagccgatg ttcttgtcca cgatattgac ctcgatgccc
     2941 atttcgccaa gggtcctgtt gacaaactcc ctgaggaccc gtccgagttg tatctccgag
     3001 agcttgagtt cgcctttttc gttgcgttcc acctcttcgt acttgccaag ctcctcgaga
     3061 tcgaattttt ccgatattcc ttcggcgagg atagcaacgc cgtaatccct gtccatgctt
     3121 tttcgtttca cgatggagcc cgtgaggata tcggcgacct ttttgaaaga aagcttttcc
     3181 tcggggaatt cctcgggtat gagtgacagg gtcgcccccg ccgctttgcc catgcccagc
     3241 gccagatgtc ccgcgtgtct tcccatcgtc gtgatgaaat accagcggcc catggtgcgg
     3301 gcatcctcga tgatgttgtt gacgatgtag acccccacgt ggcgggcggt ctggaaaccg
     3361 aaggtggaga agccgccggg aaggggtatg tcgttgtcga tggtcttcgg ggtgtggacg
     3421 acgctgatag tgccccgggc ctcgcgctct atccagttcg ccatgtagag agtgccgtcg
     3481 ccgccgatgg tgatgaggta cttgatgccc gcgcttttca acgtcgccat caaggtcttg
     3541 aacttcgcct tggcgttctc aggtttgtcc cgcgacgtcc tgaggatgga ccctccggac
     3601 gcgtggatgc gggagacatc gtcgatggtc aggggcatga atatgtcctt atgcccttcg
     3661 aagagggcct tgaaaccgcc cttgataccg acgaccttct tccccgaatt gatggcctcg
     3721 atggttgctg cgctgatgac gccgttgata cccggcgcgg gtccgccgcc gacaattatt
     3781 ccaatggttt ctgcgttcat ggtctacatt gtgcatcttt ccacctttct ttgcaagggt
     3841 ttttgtatta tcctgtttcc ggtatcgggg ccgcgatgaa gatcggtttc catgtctcca
     3901 tagcgaaggg attcgacgac acgctgaagg gagcgaaagc gctcgggtgc gaggtggcgc
     3961 aggtctttgt caagaacccg aggtcctggg aacaaagggt gctgtccgac gccgacctcg
     4021 cggcctttgg ccgcctgtcg gcggagctgc ccgttttcgc ccacctgtcc taccttccca
     4081 acctggcgaa gatcgacggg gatgagcgga acctcaaggg gctcctccac gaggccgccc
     4141 tttgcgaaaa gctggggatc gggtccctcg tcgttcaccc cggctcccgt cccgacaggc
     4201 tctcgggtgc gagaatgacc gccgaggcga tagaccgtgt tcatgaccgc catgatgtca
     4261 cggtcctcat cgagaacacg gccgggcagg ggaacgtcat aggaagggac gtcgaagagc
     4321 tggcacgcat gtacgagggc gtttccaaca aggagaagac cctgttctgc atcgatacgg
     4381 cgcacctgtt cgaatcggga tgggacataa ggaagaaagg cgtctggaag ggattcctcg
     4441 acgaattcga gaggacactg ggcctcgaca ggatcggtct cttccacctc aacgattcaa
     4501 aggcggcggc cggctcccac gccgaccgtc actggcacat cggccagggt gagatcggcc
     4561 tcacgttctt ccggatgctc gtgagggata aaagatttgc ccatctgggc ggcgtcatgg
     4621 aaacgccgaa gatgggcaat atggacgaag tcaatatgga ggttatgcgc tcgctacttc
     4681 ctccgctggt gccgcgttct tcttcttagt tttttatatt tgtgcttcct gatcttcttc
     4741 ttcctccact tcatcacact gcccattgct ccacctcttt tggtatatga aagtcaaaaa
     4801 ctgtttgtcc agagttcgtg aacgctaaca acataacctt actccgctat tttttcaagc
     4861 agataatgaa ggcaccacca ttttgcctca ccccgccact cctcccgatt atattgaatt
     4921 cagaaggcaa aaccgctata atagtccacc acgcgcctgt agctcagttg gatagagcaa
     4981 cggacttcta atccgtaggt cgcacgttcg agtcgtgcca ggcgcgcttg ttatttcaat
     5041 aggttacgag gcattcgggg ggaggggaga aaagctgtgc tacccccagt gcgaccctgg
     5101 aaggggtttc tcagagattt ctgaaggtct tatccgcata gaatatagat caccgtaaag
     5161 actctcacct tttaccgcac atcgcacgat ctacgggaag aagctgtgct gcccggagag
     5221 ggtagcatgg aggttacgct gtcgaccaga ttccattgga gggacaatgc agagagacgc
     5281 ggcggtggag acgccgcgtt gcctttcaga caaagagatg tgacctggga cccccccgtt
     5341 ccgggtccct ccccaggtcg tgaaaggcag tgtgttgttt gtagtatcgt tcttctgatg
     5401 attgcaaaca tacacgctcc cgtgaccatc ggagcgcgag aaaaagatac ttcctcaggg
     5461 gaaaaacggg ggatttccct ctggagaggt ggttctttgt ttcttcgcgc gttagcgtgt
     5521 gcctttaata aagaaccgcg gaaaatgcca actacaggtt gcctttaacg acgtggggag
     5581 gacccacaca tgggggggaa cccacgtcaa atttctttgt ctgaaaggca acgcgccctt
     5641 gtagctatga ggcgcgtact tttgagagag aacgagctta cattaagggg gtggggggca
     5701 gtactctata ttaatactca ttatacaaac tcccctagcg gaagttccca aaatggaact
     5761 tcggctgact caaacaatat ccttcagaac cttcaccact tccccggcga cattcagttc
     5821 acccacgggg c
//
LOCUS       DAOPOA010000046         5735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_15356_length_5735_cov_3.708275, whole genome shotgun sequence.
ACCESSION   DAOPOA010000046 DAOPOA010000000
VERSION     DAOPOA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15356_length_5735_cov_3.708275"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PKJ17_01730"
     CDS             complement(<1..616)
                     /locus_tag="PKJ17_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74716.1"
                     /translation="MSTQERSRKKPLTRAMRSDGEPLGIKEYEMSGGYEGLGKALRDM
                     SPHDVIEEVTASNLRGRGGAGFPTGMKWSAVPPDDGSAPHRYVISNSDEMEPGAFKDR
                     ILMEGNPHQLVEGLIIAGYATQSDRGYIFLRWAYGRSAQRLKRAIEEAYGAGYLGHNI
                     LNSGYSLDIYLHISAGRYICGEETALLNSIEGKRAIPRGRPPFPQ"
     gene            complement(616..1080)
                     /gene="nuoE"
                     /locus_tag="PKJ17_01735"
     CDS             complement(616..1080)
                     /gene="nuoE"
                     /locus_tag="PKJ17_01735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOD74717.1"
                     /translation="MLSEDERMEILKELEGAEDAPSIAMEALRIVQGRRGYISDETLC
                     DIAAFLNMTPHELDSLATFYSFIFRKPVGRHVIYVCDGVTCWVMGHESIFEHLKQRLG
                     INWGETTEDGSFTLLPVSCIGACDHAPAIMIDSTLYGDLSKEKIDEILKEHR"
     gene            complement(1106..2845)
                     /gene="nuoC"
                     /locus_tag="PKJ17_01740"
     CDS             complement(1106..2845)
                     /gene="nuoC"
                     /locus_tag="PKJ17_01740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF008728.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HOD74718.1"
                     /translation="MNTADISERKDVVQEIRDRFGDGIMAQQPARDGIPTVWVANDRV
                     KDVLKYLKHETAGPYRMLYDLTAIDERFREMRQGQPDSTFTLVYHLLSHERNSDVRIK
                     VPLTGESPRAGSITDIWQAANWYEREVWDMFGIHFDGHPELRRILLPLTWTGHPLRKD
                     HPARATDMGPFSLPDDKAKREEDALRYSPDGVKDVMLLNMGPHHPGTHGLLRMILELD
                     GEQIVNATPEIGFHHRGAEKMGERQSWHTYIPYTDRVDYLAGPLNNFPYVLAVETLAG
                     IEVPDRAKVIRIMVAELFRISNHLVYYGTFSQDVGTMSPVFFMFNDRERVFHIIEAIC
                     GGRMHPEWFRIGGVAQDLPKGWDGLVRDFISSMPRRLKEYDGLVLKNRIFKGRTRGVG
                     VLTVDEAIDRGVTGPNLRACGLEWDLRKKRPYSGYDQFAFDVPTMPDGDCYSRAVIRV
                     EEMRQSLKIIEQCLKHMPDGPYKSDHPLATPPRKDRILCDIETLIHHFLSVSWGPVIP
                     AGEAAFGTEGAKGNNTYYLISDGGAMSYRTRIRTPSFPHLQVVPFLTRGLMLSDLIAI
                     LGSIDYVLADVDR"
     gene            complement(2842..3423)
                     /locus_tag="PKJ17_01745"
     CDS             complement(2842..3423)
                     /locus_tag="PKJ17_01745"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOD74719.1"
                     /translation="MDEAVGKNIVLSRLEDLVAWGRKNSIWPFNFGLSCCYVEMAVSL
                     TSRYDIARFGAEVIRASPREADLMIIAGTVFIKVAPVIRRLYDQMMEPRWVISMGSCA
                     NSGGMYDIYSVVQGVDKILPVDVYVPGCPPSPDAFMEGLLLLQRMIASQVRPLSWAAG
                     PQEVTGIEKPSMRDLKRPLRQKATMMPSPDEAL"
     gene            complement(3490..3816)
                     /gene="ndhC"
                     /locus_tag="PKJ17_01750"
     CDS             complement(3490..3816)
                     /gene="ndhC"
                     /locus_tag="PKJ17_01750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOD74720.1"
                     /translation="MITVSFFLGERHEGRVAGEPYESGMPLTAVPQLRFHVKYYLVAM
                     FFVIFDAEALFMFAWALSVRELGLPGYIAFLVFVFVLMIALAYLWKRGALDWTDEKRK
                     SALTRT"
     gene            4259..>5735
                     /locus_tag="PKJ17_01755"
     CDS             4259..>5735
                     /locus_tag="PKJ17_01755"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOD74721.1"
                     /translation="MNDRPAREYLEKLAKLIRYYILASTTRAGSGHPTSSLSATDLMT
                     GLLFGGTFRFDAPHPANPNNDRIIFSKGHASPLFYAMWAAAGQVSFDELMTLRQFGSR
                     LEGHPVVAFPYTEASTGSLGQGLAIGLGMALNGKYLDKLPYRTYVLLGDSEMTEGSQW
                     ETIQLAAHYKLNNLIGIIDVNRLGQRGETMYGHNLKAYEERIAPFGWETMCIDGHNLD
                     EIVDAYEKARSLVDKPVMIIARTIKGKGVSFLEDKDGWHGKAISEEQFEQAMQEMGAV
                     DRSLAGKIGPPDDVKPEAVHARNIAEPDYAVETPVATRRAYGNALKRIADGFPNIVAL
                     DGEVSNSTYSEIFQEAYPERFFEMYIAEQNMMGAAAGLAARGKIPFVSTFAAFLTRCF
                     DQIRMNQYSKSNVKISGSHAGVSIGQDGPSQMGLEDIAMFRTLLDGVVLYPSDAVSTE
                     RLIEQAAAHKGVVYIRTTRMNVPILYKMDDEFTIGGSKVLRI"
BASE COUNT         1217 a         1693 c         1581 g         1244 t
ORIGIN      
        1 cctggggaaa aggcggcctt cccctcggta tggcccgttt tccctcgatc gaattgagta
       61 gtgctgtctc ctctccgcag atataccgcc ccgcgctgat atggagatat atgtcgagac
      121 tgtatcccga attgaggatg ttatgtccca gataccccgc cccgtatgcc tcttcgatcg
      181 cacgtttcag cctttgtgcg gaccgtccgt atgcccatcg cagaaagatg tagccccggt
      241 ccgactgcgt ggcataccct gcgataataa ggccctcgac aagctgatgg gggttcccct
      301 ccatgaggat cctgtccttg aacgcacccg gctccatttc gtccgaattg gaaatgacat
      361 accggtgcgg ggcggaaccg tcgtcgggcg gcacggcgct ccatttcatc ccggtgggaa
      421 accccgcgcc tcccctgcca cgaaggttgg atgccgtgac ctcctcgatc acatcatggg
      481 gagacatgtc cctgagtgct ttgcccaggc cttcgtagcc gccggacatt tcatattcct
      541 ttatgccgag cggctcgccg tcactgcgca tcgctcgggt taggggtttt ttcctgcttc
      601 tttcctgcgt actcatcacc tgtgctcctt cagtatctcg tctatctttt ccttgctgag
      661 gtcaccataa agagtgctgt cgatcatgat cgccggtgca tggtcgcagg cccctatgca
      721 gcttacgggc aggagtgtga acgagccgtc ttcggtcgtc tctccccaat tgataccgag
      781 gcgctgcttc aggtgttcga agatgctctc gtggcccatg acccagcagg tcacgccgtc
      841 acagacatag atcacatgtc tgccgacggg ctttcgaaaa atgaatgagt agaaagtggc
      901 gagactgtcg agctcatgcg gtgtcatgtt gaggaaggcg gcgatatcgc ataaggtctc
      961 gtcggaaata tagccgcgcc taccctgcac gatcctcagc gcctccatgg caatggacgg
     1021 cgcgtcctcc gcaccctcga gttctttcaa tatctccatt ctctcgtctt cgctcaacat
     1081 ctgtctcctc cgtactgtcg ccatttcagc ggtcaacatc ggcgagcacg taatcgatgc
     1141 tgccgagtat cgcgatgagg tccgaaagca tgagccccct ggtcaggaaa ggcaccacct
     1201 gcaaatgcgg gaaagacggc gtccggatcc gtgtccggta tgacatcgcg ccgccgtcgc
     1261 ttatcaggta atacgtattg ttcccttttg cgccctcggt cccgaatgcc gcctccccgg
     1321 ccggtatcac cggcccccag ctcacgctca ggaagtggtg gatcagggtt tcgatatcac
     1381 acagaatcct gtccttgcgc ggcggagttg ccaggggatg gtccgacttg tatgggccgt
     1441 ccggcatatg cttcaagcac tgttcgatga tcttgaggct ctggcgcatc tcctcgacgc
     1501 ggataacggc gcggctgtaa caatcgccat cgggcatggt gggtacgtcg aatgcgaatt
     1561 gatcgtatcc cgagtacggg cgcttcttcc tgaggtccca ctcgaggccg caggccctca
     1621 ggttcgggcc agtcactccc ctgtcgatag cctcatccac ggtcaggacc ccgacgcccc
     1681 tcgtgcggcc cttgaaaatg cggttcttca agacgaggcc atcgtattcc ttgagccggc
     1741 ggggcataga ggaaatgaag tcccgcacca acccatccca ccctttcgga aggtcttgcg
     1801 ccacgcctcc tattcggaac cattcggggt gcatgcgccc tccgcagatc gcctcgatga
     1861 tatggaagac acgctccctg tcgttgaaca tgaagaacac cggtgacatg gtccctacgt
     1921 cctgcgagaa cgtgccgtaa tagacaaggt gattggagat cctgaaaagc tcggcgacca
     1981 tgatgcggat caccttcgcc ctgtcgggca cttcgatccc ggccagtgtc tccaccgcaa
     2041 gaacatacgg gaaattgttc aatggccccg caagatagtc gacgcggtcc gtgtagggga
     2101 tataggtgtg ccacgactgg cgctcaccca ttttttcggc gccgcgatga tgaaagccga
     2161 tctcaggggt ggcgtttacg atctgttcgc cgtcgagttc caggatcatc ctcaagagac
     2221 cgtgagtgcc cggatgatgg ggccccatat tgaggagcat cacatctttg acaccgtcgg
     2281 ggctatagcg cagtgcgtct tcttcccttt tggccttatc gtcgggcagg gaaaacggtc
     2341 ccatatcggt cgccctggcc gggtggtcct tgcggagcgg atggcctgtc cacgtgagtg
     2401 gcagaaggat gcgccgcagc tccgggtgcc cgtcaaagtg gatcccgaac atgtcccaga
     2461 cttcgcgctc ataccagttg gccgcctgcc agatgtcagt gatgctcccc gcccgtgggc
     2521 tctcacctgt cagggggacc tttatccgaa cgtcgctgtt tctctcgtgg gaaaggagat
     2581 ggtagacgag tgtgaaagtt gagtccggct ggccctgcct catctccctg aagcgctcgt
     2641 caatggcggt aagatcgtag agcatccggt acggaccggc cgtttcgtgc tttaagtatt
     2701 tgaggacatc ctttacccgg tcatttgcca cccaaacggt gggaatgccg tcccgggcag
     2761 gttgttgcgc catgatgcca tcaccgaaac ggtcgcgtat ctcctgcaca acatccttcc
     2821 tttctgatat gtcagcggta ttcatagcgc ctcgtcaggg gatggcatca tggttgcctt
     2881 ttgtctgagt ggccttttca ggtcccgcat cgatggtttt tcaatcccgg tcacttcctg
     2941 gggaccggcg gcccatgaca gggggcgcac ctgtgaggcg atcatccttt ggaggagtaa
     3001 aaggccctcc atgaaggcat ccgggctcgg ggggcaaccg ggcacgtaca catcgacggg
     3061 caatatcttg tcgacgccct ggaccacgct gtaaatgtca tacatgccgc ccgagtttgc
     3121 gcatgatccc atcgatatca cccagcgcgg ctccatcatc tgatcgtaaa gcctcctgat
     3181 cacgggcgct accttgatga acacggtgcc tgcaatgatc atcaggtccg cttcacgggg
     3241 ggatgcacgg atgacctcgg cgccgaagcg cgctatgtcg tatcggctcg taaggctaac
     3301 cgccatctcg acgtaacagc acgaaagacc gaaattgaat ggccatatcg agttcttcct
     3361 gccccaagca accaagtcct cgagccggga aaggacgatg tttttgccga cggcctcgtc
     3421 catggagccc gctgtcgtaa tgccgggcga gagaccttgc cgcagtgact ttttcatggc
     3481 acgctctttt caagtcctgg ttaacgcgga cttgcgtttt tcatccgtcc aatcgagcgc
     3541 cccccgcttc cacaggtacg caagtgcgat catgagaacg aagacaaaca ctaggaaagc
     3601 gatatagccg ggcagcccca gttccctgac tgaaagcgcc caggcgaaca tgaatagtgc
     3661 ctcggcatcg aagatgacga aaaacatggc aacgagatag tattttacgt gaaagcgcaa
     3721 ctgagggacg gctgtaaggg gcattccgga ttcgtacggc tcacccgcca cacgaccctc
     3781 atgtctctcg ccgagaaaga atgacacagt tatcatcgcc gcaacgatgg ccaccacgca
     3841 gcaaaaaaag aagagtagag gccaaaaaca atacacttca agacccaaga tcacctcaca
     3901 gtttctcaaa gccgctgtcg ttcacgacga accatgcccg atccttcgcc ctattccatg
     3961 aaagacgcta atgcacctcg ctggtaccgt caacccgcga taccctgtgc cgtacgtaat
     4021 cctgccacca tcgcgtgtcc aagcaccgcc gtcctatccg tgcccttgaa acttcacaag
     4081 caccgcctta ggttcatttg agcaagacga gccattattc gagctcacga tatggaggtt
     4141 tgattggtgg aacctcgtca ttccatcaga cggcatatac gggacaggtg ctctctgccc
     4201 cgcaacatcg atcgtgaaaa caccgcgttt tcattcaatg accttcagga gaattaccat
     4261 gaatgacaga ccggccaggg aatacctgga aaagctggct aaactgatcc ggtactacat
     4321 ccttgcatcg acgaccaggg cgggttccgg ccatcccacc tcatcgctgt cggccacgga
     4381 cctgatgacc gggcttctct tcggcgggac gttccggttc gacgctcccc atccggctaa
     4441 cccgaacaac gaccggatca tcttttcgaa aggccatgcc tctcctttgt tttacgcgat
     4501 gtgggccgct gccggacagg tatccttcga cgagctcatg accttgaggc aattcggaag
     4561 ccgcctcgag ggccatccgg tcgtggcctt cccgtacacg gaggcgtcga caggctccct
     4621 tgggcaggga cttgcaatag gcttggggat ggccctcaat ggaaaatacc tcgacaagct
     4681 gccgtaccgc acctatgtgc tcttaggaga cagcgaaatg accgaagggt cacagtggga
     4741 gaccatccag ctcgcggcgc attacaagct caataacctt atcgggatta tcgatgtgaa
     4801 ccgcctgggc cagcgggggg agaccatgta cggacataac ctcaaggctt acgaggagcg
     4861 catcgcgccg ttcggctggg agaccatgtg catcgacggt cacaacctgg atgagatcgt
     4921 cgatgcgtat gaaaaagccc ggtccctggt ggacaagccc gttatgataa tcgccaggac
     4981 catcaagggc aaaggtgtct ccttcctcga ggataaggac ggctggcatg gcaaggcgat
     5041 cagtgaagaa cagttcgaac aggcgatgca ggagatgggg gccgttgaca gatctctcgc
     5101 cggaaagata gggcccccgg atgatgtgaa gcccgaggcg gtccatgcgc ggaacatcgc
     5161 tgagcctgat tatgcggtcg aaacccctgt ggcaacccga agggcttatg gcaatgccct
     5221 caaaaggatc gcagacggat ttcccaatat cgtggcactc gacggggagg tcagcaattc
     5281 cacatactcg gagatctttc aggaggcgta tcccgagcga ttcttcgaga tgtacatagc
     5341 ggagcagaat atgatgggtg cggccgcggg tcttgcagcc cgcggaaaga ttccgttcgt
     5401 ttctaccttt gcggcatttc taacgcgatg cttcgaccag atcaggatga atcagtattc
     5461 gaaaagcaat gtcaagatct cgggttcgca cgccggtgta tccataggac aggacggccc
     5521 ttcccagatg ggccttgaag atatcgccat gttccggacc ctgctcgacg gtgttgtcct
     5581 gtatcccagt gatgccgtgt ctaccgagag gctcatcgag caggccgcgg cgcataaagg
     5641 ggttgtctat atccgcacga ccaggatgaa tgtgcccatc ctttataaga tggacgacga
     5701 gtttaccata ggcggaagca aagtcctacg catac
//
LOCUS       DAOPOA010000047         5673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_15587_length_5673_cov_3.287647, whole genome shotgun sequence.
ACCESSION   DAOPOA010000047 DAOPOA010000000
VERSION     DAOPOA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15587_length_5673_cov_3.287647"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="PKJ17_01760"
     CDS             <1..1234
                     /locus_tag="PKJ17_01760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOD74722.1"
                     /translation="LIGRVIDPQGRVLDGQGPLRSTRRRPIEKAAPAILDRLPVTVPL
                     ETGLKVIDALIPIGRGQRELILGDRQTGKTAIAVDAMINQGGKGVVCIYCAIGKRGSA
                     VAKVVADLNQYGAMGHSIVVVATEEQPPGLQFTAPYAAMTIAEFLMGRGRDVLVVFDD
                     LTRHARAYRELSLLLRRPPGREAFPGDIFYVHSRLLERSTHLKEELRGGSITALPVVE
                     TEAQNISAYIPTNLISITDGQIYLSPDLFQKGVLPAVDVGRSVSRVGGKTQFHAYRVV
                     AGDLRLAYSQFVELESFARFGTRLDEETRKTLERGRRVREVLKQPQYSPMKASEHIAV
                     LLAVNEGLLDDLPLEEIADVEKKICRAVTAQLPDLCEKIERGGKLTQQDQESILGAAR
                     AIPGIRETRQDGPEGEGE"
     gene            1238..2140
                     /locus_tag="PKJ17_01765"
     CDS             1238..2140
                     /locus_tag="PKJ17_01765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOD74723.1"
                     /translation="MLTLTGLNRRIRSTEDLQSVVRTMKALAAVSIRQYEKAVESLRD
                     YSRAVDMALQVILRSVPGTAIRARRVPGKKVGAIVFGSDHGMCGPLNEQVVSHAVASM
                     DDSDAGEKVILAVGVRVRDRMGDAGYPIEHHLPVPGSVAGIGPGVRDLLLEIERWQSV
                     KGIGRILLFHSVLEGGASYHPREFLLLPVDEEWLAGLRKRSWPTRVLPTFTGDREALF
                     SAVIREYMFISLFRAFAESLASENASRLASMQAAEKNIEDQLSELRRTFHQMRQMTIT
                     EELLDIVSGFEALTGGKGNAKIRE"
     gene            2157..3113
                     /locus_tag="PKJ17_01770"
     CDS             2157..3113
                     /locus_tag="PKJ17_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HOD74724.1"
                     /translation="MEHVITLTMNPSIDISSAVGQVVADRKLRCGPSARGPGGGGINV
                     SRAIGKLGGTSSALYPAGGALGEMLDGFMEREGIAYRSIFTEGSTRENITILEESTGR
                     QFRFGMTGPTLKEEEWRQCLKVLSYLSPKPGYIVASGSLPPGVPEDFYARVADVASQL
                     GARLIVDTSGEPLRFALQAGVYLLKPNVREMKDLVGHEIGNEYELEDSARELIGKGGT
                     ETIVVSLGAEGALLVSGDGCEHVRAPSVPVRSVVGAGDSMVAGIVLGLARRKELGEAV
                     SFGVAAGAAAVMSPGTGLCCRGDTERLFRQVVINEHASLKGE"
     gene            3116..4375
                     /locus_tag="PKJ17_01775"
     CDS             3116..4375
                     /locus_tag="PKJ17_01775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD74725.1"
                     /translation="MPDRHRIVQIGDYEQFVGPETIGRILEKARKLQHLHVANVNSTY
                     YGGGVAELLTSLTLLMNSAGIKTGWRVIQGAPDFFSITKKMHNALQGGDINLSDRKMD
                     IYEEVIYENVIRNHLDDHNMVIIHDPQPLPMVDGYRKNGPWIWRCHVDLSNPNRELWD
                     YLVPYIEKYDAVILSIKDYKKKLKTPQVFFMPAIDPFSILNRDMTEGEVEERLERYGI
                     PTDLPLVVQISRFDRWKDPQGVIEAYKIARKEVDCTLVLLGNVATDDPEGPEVYQSLL
                     ECREERILILSHQDTALVNALQRKAAVVLQKSIREGFGLTVAEAMWKGTPVIGGNVGG
                     IKYQIDNNTNGFLVSDIEDAAGRIVQVLRDRKLRERLGKAARESVRKKFLLTRYLEQY
                     LDLFHSFETQYRLDTRAVKKKWRRARN"
     gene            4581..4946
                     /locus_tag="PKJ17_01780"
     CDS             4581..4946
                     /locus_tag="PKJ17_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOD74726.1"
                     /translation="MTILEVSFREMEGDKSAERTIREKVRKLVEAFPRIRICRITLEG
                     RQRRQRSGKRYRIRIVMALPEREFVVAGESKGSEACEPLTRVLADTFSRARRVLREHT
                     KRLREEIEMASDRRATRWP"
     gene            5090..5166
                     /locus_tag="PKJ17_01785"
     tRNA            5090..5166
                     /locus_tag="PKJ17_01785"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5124..5126,aa:Lys,seq:ctt)
     gene            complement(5197..>5673)
                     /locus_tag="PKJ17_01790"
     CDS             complement(5197..>5673)
                     /locus_tag="PKJ17_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HOD74727.1"
                     /translation="LRETMKMIGPAAIALFFALAMVYVMIHSGSASPRDSMLLVMAEA
                     AADTVGGIWYLAAPLVGALGSFISGSNTVSNIMFGVFQLDTAEQIGLPAAPVLALQAV
                     GGAAGNMIAVHNIVAVLTTVGLLGKEGIVIRKNLPVALAYALVTGLAGWILISVVS"
BASE COUNT         1248 a         1519 c         1845 g         1061 t
ORIGIN      
        1 ccttatcggc agggtgatcg acccgcaggg gcgcgtcctt gacggtcagg gtccccttcg
       61 ctccacgcgg cgccgtccca tagaaaaggc cgccccggcc atcctggaca ggctgcccgt
      121 gaccgttccc ctcgagacgg gtctcaaggt gatcgacgcg ctgataccga taggaagggg
      181 gcagcgcgaa ctcatactgg gagatcgtca gacggggaag acggcgatcg cggttgatgc
      241 catgatcaat caggggggca agggtgtggt ctgcatctat tgcgccatcg gcaagcgggg
      301 ttccgccgtg gccaaggttg tggccgatct taaccagtac ggggccatgg gtcattccat
      361 cgttgttgtg gccaccgagg agcaaccgcc cggcctgcag ttcacggcgc cctatgcggc
      421 gatgacgata gcggagttcc ttatgggcag gggaagggac gtgctggtgg ttttcgacga
      481 cctcacgcgt catgcccggg cttaccggga gctctccctt ttgcttcgca ggcctccggg
      541 cagagaagcc tttccaggcg acatcttcta tgttcactcg cggcttcttg aacggtccac
      601 gcatctgaag gaggagttga ggggaggttc gatcaccgcc ctgcccgttg tggaaacgga
      661 ggcccagaac atctcagcct acatccccac caacctgatc tcgataaccg acgggcagat
      721 ctacctctct ccagatctct ttcaaaaggg tgtcctgccg gcggtggacg tggggagatc
      781 ggtgtcgagg gtgggtggaa aaacgcaatt ccacgcgtac cgggttgtgg caggtgatct
      841 gcgccttgcc tattcgcagt tcgtggaact ggaatcgttt gcccggttcg gcacgcggct
      901 cgatgaggaa acaaggaaga ccctggagcg cggtcgccgg gtcagggaag ttttgaaaca
      961 gccgcagtat tccccaatga aggcctcgga gcacatagcc gtcctgctgg cggtgaacga
     1021 gggcctgctt gacgatctcc ccctcgagga gatcgccgat gttgaaaaga agatctgccg
     1081 ggcggtcacg gcccaactgc cggacctgtg tgaaaagata gagaggggag gcaagcttac
     1141 ccaacaggac caagagtcca tacttggggc ggctcgcgcg ataccaggga ttcgggagac
     1201 cagacaggac ggaccggaag gggaggggga ataaggcatg cttaccctta ccggtctgaa
     1261 caggaggatc cggagcacgg aagaccttca gtccgtggtc aggaccatga aggcgcttgc
     1321 cgcggtcagc atccggcaat acgaaaaggc ggtggaatcg ctccgcgatt acagcagggc
     1381 tgtcgatatg gctcttcagg ttattttgcg gagcgttcca ggaacggcca taagggcccg
     1441 gagagttccg gggaagaagg tcggagcgat cgtcttcggt tccgaccatg gcatgtgcgg
     1501 acctctcaac gaacaggtgg tctcgcacgc ggtagcgtca atggatgatt cagacgccgg
     1561 cgagaaagtc attctcgcgg ttggtgtgcg tgtgagagac cgaatggggg acgcgggata
     1621 ccccattgaa catcacctgc ccgtcccggg ttcggtagcg ggtatcggac cgggcgtgcg
     1681 ggacctgttg cttgagatcg agcgctggca atcggtaaaa gggataggca ggatcctgtt
     1741 gttccacagc gtgcttgagg gcggagcctc ctaccatccc cgtgagttcc ttctcctgcc
     1801 ggtggatgag gagtggctgg cgggcctcag gaagaggtcc tggcccacgc gggtactccc
     1861 gacgttcacc ggggaccggg aagccctctt ctccgccgtt atcagggagt acatgttcat
     1921 atcgctcttc cgggcgttcg cggagtcgct cgcgagcgag aacgccagcc ggctggcgtc
     1981 catgcaggcc gccgagaaga acatcgagga tcagctctct gaactcagga gaacgttcca
     2041 tcagatgagg cagatgacaa taacggaaga acttctggat attgtgtccg ggtttgaggc
     2101 cctcacaggg ggaaaaggca atgcgaaaat cagagaatga agaaagactc gaccacatgg
     2161 aacatgtaat tacgctgaca atgaaccctt ccatagatat cagctcggcg gtcgggcagg
     2221 ttgtcgccga tcgtaaactg cgttgcggac cgtcggcgcg cggcccggga ggagggggca
     2281 tcaacgtgtc gcgggcgatc gggaaactgg gtgggacgtc gtccgcgctc tacccggctg
     2341 gaggcgcatt aggggagatg ctcgacgggt tcatggaacg ggagggcata gcataccgct
     2401 ccatcttcac cgaaggctcg acgcgggaaa acatcaccat tctggaggag tcgacgggac
     2461 gccagttccg tttcggcatg acgggaccga cgctgaagga ggaggaatgg cggcagtgcc
     2521 tcaaggtcct gtcctacctg agtccgaaac cgggttacat cgtggcgagc ggaagccttc
     2581 ctccgggggt tcccgaggat ttctacgcca gggtggcgga tgtcgcgtcg cagctgggag
     2641 cgaggctgat cgtcgacacg tcgggtgagc cccttcgctt tgccttgcag gcgggcgtgt
     2701 atctgctgaa accgaacgtg agggagatga aggacctcgt cggtcatgag atcggtaatg
     2761 aatacgaact ggaagacagc gcccgggagc tcatcgggaa aggcgggaca gaaacgattg
     2821 tcgtctcgtt gggtgccgaa ggcgcccttc tggtgtccgg ggacggctgc gagcacgtga
     2881 gagcgccgtc tgtcccggtc aggagtgtcg taggggccgg agacagcatg gtggccggca
     2941 tcgtgctcgg cctggcgcgg aggaaggaac tcggggaagc ggtctctttc ggcgtggcgg
     3001 cgggtgccgc ggcggtcatg tccccgggga cggggctgtg ctgccggggc gacaccgagc
     3061 ggttgttccg tcaggtagtc atcaatgagc atgcttcact gaaaggagaa tagacatgcc
     3121 agaccgtcac aggatcgttc agatcgggga ttacgaacag ttcgtggggc cggaaacgat
     3181 cggccggatc ctcgagaagg cccggaagct tcagcacctt cacgttgcca atgtgaattc
     3241 cacgtattat ggcggtggag tggcggagct tctgacctca cttacgcttc tcatgaacag
     3301 cgcggggatc aagacaggct ggagggtcat acagggggct cccgatttct tcagcataac
     3361 caagaaaatg cacaacgccc tccagggcgg agacatcaac ctcagtgacc gcaagatgga
     3421 catctacgag gaagtgatct acgagaatgt catcagaaac catcttgacg accacaacat
     3481 ggtgatcatc cacgatcccc agccccttcc catggtggat ggctacagaa agaacggtcc
     3541 ctggatctgg agatgccatg ttgatctctc caatcccaac agggaactct gggactatct
     3601 tgttccctat attgagaagt acgacgcggt gatattgagc atcaaggact acaaaaagaa
     3661 gctcaagacg ccgcaggtct tcttcatgcc tgccatcgat cccttctcca ttctgaaccg
     3721 ggacatgacc gaaggggagg tcgaggagcg gcttgagcgt tacgggatac cgacggacct
     3781 gcccctggtc gtccagatat cgcggttcga tcgctggaag gaccctcagg gcgtcataga
     3841 ggcgtacaaa atagccagaa aggaggtgga ttgcaccctg gtccttctgg ggaatgttgc
     3901 caccgatgac ccggaaggtc cggaggtgta ccagtctctc ctggagtgca gggaggagag
     3961 gatcctcata ttgagccacc aggatacagc tctggtcaac gccctccaga ggaaggcggc
     4021 cgtcgtcctg cagaaatcca tccgcgaagg gttcggattg accgtggcgg aggccatgtg
     4081 gaaggggacg ccggtgattg gaggcaacgt ggggggcatc aagtaccaga tcgataacaa
     4141 caccaacggg tttcttgtct cggatatcga agacgcggca ggaaggatcg ttcaggtgct
     4201 cagggacagg aaattgcggg agcgcctggg aaaggccgcg agggaatcgg ttcgtaagaa
     4261 gtttcttctg acccgttacc tcgagcagta cctcgatctg ttccattcct tcgagacgca
     4321 gtatcgcctg gacacccgtg ccgtgaaaaa gaaatggcga cgggcacgga attgaggcat
     4381 agtgctacga ttggggcggc atgccacgca cgcctcttcg gcgatcgcat ccttccaccc
     4441 ctttcggacc ggtgaaaaga ggatgattat ccgcgggtta cctgtccgcg cgtgccgcca
     4501 ggggcctggc atccgtgagc tgggagaatg gcacgcctct tgaatgacaa acaggaattc
     4561 cacaaagacg gggtgtgctt atgaccattc tggaggtttc gtttcgggag atggaaggag
     4621 acaagagcgc cgagagaaca ataagagaaa aggtaaggaa acttgtggag gcgtttccgc
     4681 gtatcaggat ctgccgcatc acgctggaag ggcggcagcg gcgtcaacgc tccggcaagc
     4741 ggtaccggat ccgcatcgtc atggccctgc ccgaaaggga attcgttgtg gccggtgaat
     4801 cgaaggggag tgaggcatgt gagccgctga cacgggtcct ggcggatacg ttcagccggg
     4861 ctcgcagagt gctccgggag catacaaagc ggctgcggga ggagatcgag atggcatcgg
     4921 acaggcgggc cacgcgctgg ccgtgaagcc cttccgccaa gaggggcaga tttcccgggg
     4981 gcgatggccg ggagctagac gggccatcct gcaggaagga ccgcgaagaa tcggaaatga
     5041 caaaattctt gcagacaacc tcacgtctat gctataaaaa tcaatctcag ggccgttagc
     5101 tcagtccggt agagcagttg actcttaatc aattggtcca aggttcgaat ccttgacggc
     5161 ccaccaaacc aacctgaact gatttcaatt gtcacatcat gatacgaccg agataagtat
     5221 ccagcctgca agtccggtaa ccagcgcata ggcgagagcg acaggaaggt tcttcctgat
     5281 aacgatgcct tcttttccaa gaagaccaac cgtggtgaga acggccacga tgttgtggac
     5341 tgcgatcatg ttgcccgcgg caccgcccac cgcctgaagc gccagaacag gagcggccgg
     5401 caggccgatc tgttccgctg tgtccaattg gaagacaccg aacattatat tcgacaccgt
     5461 attgcttccc gatatgaacg aaccaagggc gccgaccagt ggcgcagcca ggtaccatat
     5521 cccgccaacc gtgtccgccg ccgcctccgc catgacgagg agcatggaat cgcgcgggga
     5581 ggcgcttccg gagtgtatca tcacgtagac catggccagg gcgaaaaaga gggcaatggc
     5641 ggcgggtccg atcatcttca tggtttcgcg aag
//
LOCUS       DAOPOA010000048         5636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_15735_length_5636_cov_2.823688, whole genome shotgun sequence.
ACCESSION   DAOPOA010000048 DAOPOA010000000
VERSION     DAOPOA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15735_length_5636_cov_2.823688"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKJ17_01795"
     CDS             complement(<1..283)
                     /locus_tag="PKJ17_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74728.1"
                     /translation="MAFDGIIGHERQKRFLSFLMERGNIPHAFLFCGQEGIGKKQIAM
                     EFTRRLFCQAGTGCGVCRPCVKLDRGSHPDLVVIRNEGSIGIDDSRMIAK"
     gene            complement(273..893)
                     /gene="tmk"
                     /locus_tag="PKJ17_01800"
     CDS             complement(273..893)
                     /gene="tmk"
                     /locus_tag="PKJ17_01800"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOD74729.1"
                     /translation="MLITFEGIEGSGKTTQIELLSDYLAGKGYGVVKTREPGGTRFGE
                     ALREVLLRPGMDVDSLSELLVFMAMRAQHVEEVILPALQDGKVVLCDRFVDATYAYQG
                     YGRKIDLGVIETLNRLVTKGIRPNLTILMNVTAERGLKRKAAASAMDRIEEEDISFHQ
                     RVKKAYEKLAKEDPKRFFVVDGSLKVEAIHQVVREKVGNVLKSYGV"
     gene            complement(901..1467)
                     /gene="efp"
                     /locus_tag="PKJ17_01805"
     CDS             complement(901..1467)
                     /gene="efp"
                     /locus_tag="PKJ17_01805"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOD74730.1"
                     /translation="MIVDTTDFRKGLRILQDGEPFTIVEFQHVKPGKGGAFVRTRMKS
                     LVTGNVLDKTFRSGDKVEVPEVEEKQMQFLYKEDTNYHFMDEATYDQIFIEEANLGDT
                     KNYLKESMVITVLFYKGRTIGVDIQNFVNLTITQTEPGIKGDTAQNATKPAVLETGYT
                     VQVPLFVEEGDVVRVDTRTGQYIERVQK"
     gene            complement(1510..2586)
                     /locus_tag="PKJ17_01810"
     CDS             complement(1510..2586)
                     /locus_tag="PKJ17_01810"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOD74731.1"
                     /translation="MVMIRQARIERVQSLLEETGLDGCVLKGMDNIFYLTGFRGSEGS
                     LFITRGDVVLVVDFRYVTHAREVTRDIRIEEMRPKRDVINDLCTKYRISKLGFDGAHV
                     HYNVFKSWAENLVGISLVPMNNAIEEIRKTKEPEEITAIMAAIEAATKAFTDVLGLVK
                     PGSTEKEIADELDHAMRKRGAQGPSFDTIVASGPRGALPHAEPGDRKLKEGETVIIDY
                     GAAVAGYCSDETVTVCLGEIPDKLSEIYAIVNDARKLGIEKARAGMAIARLDAIVRGY
                     IEERGYGDFFRHGVGHGVGIAVHEAPGINSVANGILEENMVITVEPGIYLPNIGGVRL
                     EDMVLITEDSPRVLTHIRKDIIKM"
     gene            complement(2580..2795)
                     /locus_tag="PKJ17_01815"
     CDS             complement(2580..2795)
                     /locus_tag="PKJ17_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74732.1"
                     /translation="MDKPVIASVTLAEIYLEQGHVERSIEVYAELVKREPFNEAYKKR
                     LAGLKKEWKTGQKKPGTLRKALGKKLW"
     gene            complement(2977..3693)
                     /gene="yqeC"
                     /locus_tag="PKJ17_01820"
     CDS             complement(2977..3693)
                     /gene="yqeC"
                     /locus_tag="PKJ17_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HOD74733.1"
                     /translation="MWHIQRTIDPSDLIEGVRFASFIGGGGKTTLIEHLARSCLERGK
                     SVAIATTTKIFIAEPFALFDDWMKTPRGAPFMHIGKTIEGPKLTALSSQEVEVLGHDF
                     DVVLIEADGAKGHPLKYPASHEPVIPPFSDRVFIVAGLDALFKPFDDAVFRHELFSRE
                     TGTEPPRLVYPDIFIRFFQDDALLKSTAGFMRTIVLNKYDTSRPRHLAAVLMQKILDR
                     TGIEQGVITAPEYGIYYRIR"
     gene            complement(3668..3922)
                     /locus_tag="PKJ17_01825"
     CDS             complement(3668..3922)
                     /locus_tag="PKJ17_01825"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOD74734.1"
                     /translation="MDRSKQVNVHTIESIFEMDMIKDALDKEDIPYTIKEHRDTAYDG
                     LFILQKGFASLYVEEGDRDRALAIVKRIRNLPYVAYSEDD"
     gene            complement(3909..4373)
                     /locus_tag="PKJ17_01830"
     CDS             complement(3909..4373)
                     /locus_tag="PKJ17_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74735.1"
                     /translation="MKKIMILCFALSLVCAGCAAFQKQGSEPAPASKTQGFNQAFYGF
                     PDVPVPKELDIVNDRSFVYETPTFKAGVIVFSGNVDPASVESYYKLNMAKNGWKYINS
                     FRYKDIVMNYAKEDKTCNIKISRGAFQTELEIWVGPADKGSFPKSAPPNGPK"
     gene            complement(4370..5161)
                     /gene="folE2"
                     /locus_tag="PKJ17_01835"
     CDS             complement(4370..5161)
                     /gene="folE2"
                     /locus_tag="PKJ17_01835"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF010200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOD74736.1"
                     /translation="MADVQNMHDSREIEIDKVGVKNVKYPIIVLDRANGHQNTIATID
                     MYVNLPHRYKGTHMSRFVEILHEYKNMINMKNFPDLLKEMKTRLDAEAAHMSVCFPYF
                     IKKEAPVSKTPSYMEYQCGFKGSMGADNTMTEFIVSVSVPINTLCPCSKEISEQGAHN
                     QRGITKVDVYTKRFFWIEDLIDIVEKSASSDIYSILKREDEKFITEHAFDNPKFVEDV
                     VRDISERLSRDTNIRWFAIEAENFESIHNHSAYAYLERNNEGGNV"
     gene            complement(5169..5537)
                     /gene="queD"
                     /locus_tag="PKJ17_01840"
     CDS             complement(5169..5537)
                     /gene="queD"
                     /locus_tag="PKJ17_01840"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOD74737.1"
                     /translation="MFTLCVKDSFAAAHRLVGYKDNCEELHGHNFAVEVYLSGDRLGD
                     DGMLVDFKTIKANIQKVLDVLDHKYINDIPFFAERASSAEYVAMYIFTEMEKLMGTER
                     VSVSRVTVWESEKACASYER"
BASE COUNT         1044 a         1566 c         1590 g         1436 t
ORIGIN      
        1 ccttggcgat catccgggag tcgtctatgc ctatggaccc ttcgttccgt atgacaacga
       61 ggtcggggtg gcttccccgg tcgagcttca cgcaggggcg gcataccccg caacccgtcc
      121 ctgcctgaca gaagaggcgc ctcgtgaact ccatggcgat ctgttttttg ccgatgcctt
      181 cctgcccgca gaagagaaat gcgtgaggga tgttgccccg ctccatgaga aagctcagga
      241 agcgtttttg cctctcgtgg cctatgatgc cgtcaaacgc catagctctt gaggacgttt
      301 cccacctttt cccttacgac ctgatggatc gcctcgacct tcagagagcc gtcgacgacg
      361 aagaaacgct tggggtcttc ctttgccagc ttttcgtagg cctttttcac tctctggtga
      421 aaggagatgt cctcttcctc tatcctgtcc atggcggatg ccgccgcctt gcgtttcaga
      481 cccctctcgg cggtcacgtt catgagtatc gtgagattgg gccgtatgcc tttggtcacc
      541 agtctgttca gggtttctat caccccgagg tcgatcttcc tgccgtatcc ctgataggcg
      601 taggttgcgt cgacaaacct gtcgcacaac accactttgc cgtcctggag cgccggaagg
      661 atgacctctt ccacgtgttg cgccctcatg gccatgaaca cgaggagctc cgacagggag
      721 tcgacatcca tgccaggtct caggagcacc tcgcgcaagg cttcgccgaa gcgcgtgccc
      781 cctggctcac gcgtcttgac caccccgtag cctttgccgg ccagataatc gctcaggagt
      841 tctatctgtg ttgttttgcc gctcccctcg atgccttcaa aggtgattaa catgggtctc
      901 ctatttctgc acgcgctcga tgtactggcc tgtccgggtg tcgaccctga ccacgtcccc
      961 ttcttcgaca aagagaggga cctgcacggt gtatcctgtc tccaggaccg cgggcttcgt
     1021 ggcgttttgc gccgtgtcac ccttgatccc cggttcagtc tgggtgatgg tgagatttac
     1081 gaagttctgg atatcgacgc ctatcgttct gcccttgtag aagagaacgg ttatgaccat
     1141 gctttcttta aggtagttct tcgtgtcccc gagattggcc tcctcgatga agatctggtc
     1201 gtaggtggcc tcgtccatga aatggtagtt ggtgtcttcc ttgtagagga actgcatctg
     1261 tttctcctcc acctcgggga cctccacctt gtcaccggat ctgaaggtct tgtcgaggac
     1321 gttgcccgtg acgaggctct tcattcgggt cctgacgaag gctccgccct tgcccggctt
     1381 gacgtgctga aactcgacaa tggtgaaggg ctcgccatcc tggagaatcc tgagaccctt
     1441 cctgaagtct gttgtgtcga cgatcatctg tttcctcctg agatcataaa acctcccgct
     1501 tatgcgtatc tacatcttta tgatgtcttt tcttatgtga gtcaggaccc gcgggctatc
     1561 ttccgtaatt agtaccatat cttcaagcct gacgccaccg atattcggca ggtagatacc
     1621 gggttcgacg gtgatgacca tgttttcctc cagtatcccg ttggccacgc tgttgatgcc
     1681 gggtgcctcg tggacggcga ttcccacgcc gtggcccaca ccgtgacgga agaagtcgcc
     1741 gtagccgcgt tcctctatgt aacccctcac gatggcgtcg agccgggcta tggccatgcc
     1801 cgcccgggcc ttttcgatcc ccagcttgcg ggcatcgttg acgatggcgt atatctcgga
     1861 gagcttgtcg ggtatctccc cgaggcacac cgtgaccgtc tcgtcgctgc agtatccggc
     1921 cacggcggca ccgtagtcga tgatgaccgt ttcgccttcc ttgagcttcc tgtcgcctgg
     1981 ctcggcatgg ggcagggcgc cccgcggacc cgaggcgacg atggtatcga aggacggtcc
     2041 ctgtgccccg cgtttgcgca tcgcgtggtc aagctcgtcg gcaatctctt tttccgtgct
     2101 gcccggcttc accaggccga ggacgtcggt gaacgccttc gtcgcggcct cgatggccgc
     2161 catgatggcg gtgatctcct ccggttcctt ggtctttctg atctcctcta tggcattgtt
     2221 catgggaacg agactgatgc cgacgaggtt ctcggcccag gacttgaaaa cgttatagtg
     2281 gacatgggcc ccgtcaaagc cgagcttgga gatcctgtat ttcgtgcaga gatcgttgat
     2341 gacgtcgcgc ttgggcctca tctcctcgat gcggatgtca cgggtcacct ctctggcgtg
     2401 cgtcacataa cgaaaatcaa cgacgaggac gacgtctccc cgggtgatga agaggctccc
     2461 ctcggacccc ctgaaccccg tcagatagaa gatgttgtcc atgcccttga gaacgcaacc
     2521 gtcgagccct gtctcttcga ggaggctctg tacccgttct atccgtgcct ggcgtatcat
     2581 caccatagtt tcttcccgag ggcctttcgc agtgttcccg gtttcttctg cccggtcttc
     2641 cattccttct tcagcccggc gaggcgcttc ttgtaggcct cgttgaaggg ttctcttttc
     2701 accagttccg cgtagacctc tatggacctt tcgacatgcc cctgctccaa gtatatctcc
     2761 gccagcgtga cggacgcgat aaccggtttg tccataaggc ataaatatat gaatatattg
     2821 ggtaaaaagc aaggaaaatc aggctggtcc aggcaaaaga cagttgcggg ttccgggttg
     2881 cgtgtttcga gtcaaagacc aaagtgggac agttctcctg tatttcatcc tgaaatgtgg
     2941 aactgttccc cctgtcttct tcctgagacc cggggactat cgtatcctgt agtagatccc
     3001 gtattctggc gcggtgatca cgccctgttc tatacccgtg cggtccagta tcttttgcat
     3061 gagcacggcg gcgagatggc gggggcggga cgtgtcgtac ttgttgagga ctatggtgcg
     3121 catgaagccg gccgtggatt tgaggagggc gtcgtcctgg aagaagcgga tgaagatgtc
     3181 ggggtagacg aggcgcggag gttcggtccc cgtctcgcgg ctgaagagct catggcggaa
     3241 gaccgcgtcg tcaaatggct tgaagagggc gtcgaggccc gccacgatga agaccctgtc
     3301 cgagaaaggg gggatgacgg gttcgtgcga cgccgggtac ttgagggggt gaccctttgc
     3361 cccgtcggcc tcgatgagga cgacatcgaa atcgtggccc aggacctcca cttcctgaga
     3421 cgatagtgcc gtcagcttgg gtccctcgat cgtttttccg atgtgcatga aaggggcgcc
     3481 acggggcgtt ttcatccagt cgtcgaaaag cgcgaagggc tcggcgatga atatcttcgt
     3541 tgtcgtggcg atggccacgg acttgcctcg ttcgaggcag gaccgggcga gatgctcgat
     3601 cagggtcgtc ttgccgcctc ccccgatgaa ggaagcaaac ctgacgccct ctatgaggtc
     3661 tgatgggtca atcgtcctct gaatatgcca catagggaag gtttctgatc cgtttgacaa
     3721 tggcaagggc acggtctctg tcgccttctt ccacgtagag cgaggcgaaa cctttctgga
     3781 ggatgaaaag gccgtcgtag gcggtgtccc tgtgttcctt gatggtgtag ggaatgtcct
     3841 ctttatcgag agcgtccttg atcatatcca tctcaaagat gctctcgatg gtgtggacgt
     3901 ttacctgctt acttcggtcc atttgggggg gcggatttcg ggaacgatcc cttgtcggcc
     3961 ggaccgaccc agatctcgag ctcagtttga aatgccccgc gggatatctt gatattgcac
     4021 gtcttgtctt ccttggcgta gttcatcaca atgtctttgt agcgaaagct gttgatgtat
     4081 ttccagccgt tcttggccat gttgagcttg tagtagctct caacagaggc gggatcgacg
     4141 ttgccgctga agacgatgac ccctgccttg aaagtgggcg tttcgtagac gaaactgcgg
     4201 tcgttgacga tatccagctc cttcggaacg ggcacatcgg gaaagccata gaaggcctgg
     4261 ttaaatccct gggtcttgga ggccggtgcg ggttcggacc cctgtttttg gaaggcggcg
     4321 cacccggcac acaccaggga cagagcaaaa cacaggatca tgatcttttt catacattcc
     4381 ctccctcgtt gtttctttcg agatacgcgt acgcgctgtg gttgtgtatg ctttcgaagt
     4441 tctccgcctc gatggcaaac cagcgaatgt tcgtatcccg tgataatctc tcggatatgt
     4501 ccctcaccac atcctcgacg aattttgggt tgtcgaaggc gtgctcggtg atgaacttct
     4561 cgtcttctct ctttaggata gagtatatat cactgctcgc acttttttct acaatatcga
     4621 tgaggtcctc gatccaaaag aaccgtttcg tgtatacatc cacctttgtg atccctctct
     4681 ggttgtgggc cccctgttca cttatctcct tggagcaggg gcagagcgtg tttatgggga
     4741 cagagacgga aacgatgaat tccgtcatgg tgttgtccgc tcccatggaa cctttgaatc
     4801 cgcactggta ttccatgtag ctcggcgtct tcgacacggg ggcctctttc ttgatgaaat
     4861 aagggaagca cacggacatg tgggcagctt ccgcgtcgag gcgcgtcttc atctccttca
     4921 agaggtcggg gaagtttttc atattgatca tgttcttgta ttcatggagg atctccacga
     4981 acctgctcat gtgcgtcccc ttgtagcgat ggggaaggtt cacgtacatg tcgatggtgg
     5041 cgatggtgtt ctgatggccg ttcgcccggt cgagaacgat gatggggtac ttgacgttct
     5101 tgacccccac cttgtcgatc tcgatctcgc ggctgtcgtg catgttctga acatcggcca
     5161 ttttctgtct atcgttcata gcttgcacag gccttttcag attcccagac ggtgaccctg
     5221 cttacggaca cccgctccgt gcccatcagc ttttccatct ccgtgaagat gtacatggcc
     5281 acatattcag ccgagctggc ccgctcggca aagaacggta tgtcattgat atatttgtgg
     5341 tccaggacat cgaggacttt ctggatgttc gccttgatgg tcttgaagtc gacgagcatt
     5401 ccgtcatctc ccagccggtc gccgctcagg tagacctcca ccgcaaaatt gtggccgtgg
     5461 agttcctcgc aattgtcctt gtatccgacc aggcggtgag cggccgcaaa tgaatccttc
     5521 acgcagagtg tgaacatgcc ataagatatc gtctgaaggg gggaaagtca agaaggacag
     5581 gggacaggga acaggggagc agggggagag aggcggggtt ttgataggac gaatag
//
LOCUS       DAOPOA010000049         5612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_15830_length_5612_cov_3.947634, whole genome shotgun sequence.
ACCESSION   DAOPOA010000049 DAOPOA010000000
VERSION     DAOPOA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15830_length_5612_cov_3.947634"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1141)
                     /locus_tag="PKJ17_01845"
     CDS             complement(74..1141)
                     /locus_tag="PKJ17_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74738.1"
                     /translation="MDELVRAIDSAQDLEKKVEYYFTLTGDERVSVLEGLGKERNEET
                     GVLLNAIYLQETDKEIRKLIRKTIFRLRSVGVKVEDLRPEGEPVLRKAEESRTNMGFL
                     TNFDHAGSRMVMAAYEVRKNTFVFLNGDLHFREGLRELMSSPVDRRSLNEIVAAYRSG
                     TKEPAFLVEISPSYAAFIVEEGARLSGRFTDAIQSLASFVAQLKDTVHKPDDIHSLPV
                     ADDVAPQPLHEVLRHEIFTPFSLSWEGIEGDRASYLSTGGSTIVLPRHMMEEKKDSYL
                     RDLVGRQDIASLAPLFRRLLEDYAYLFHCMGDGARYKGTLSILRDKTAFDHALSFFLR
                     KSLETGQEGPSESDLIINPYG"
     gene            complement(1169..1372)
                     /locus_tag="PKJ17_01850"
     CDS             complement(1169..1372)
                     /locus_tag="PKJ17_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOD74739.1"
                     /translation="MAEAKLKIDGMSCQHCVMAVKKALGAVAGVDSSDVDIGSAVVRY
                     DEGKASQKDLEAAVEKAGFSVKH"
     gene            complement(1506..3116)
                     /locus_tag="PKJ17_01855"
     CDS             complement(1506..3116)
                     /locus_tag="PKJ17_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOD74740.1"
                     /translation="MIHYVFLFLAGLALFLLGMLKLSEQMQRVFSSRIREYIRFSVRK
                     PFYGLITGFVTTIFFQSSSATTLLTMGLVSAGLVSFYHSLGIILGADIGTTLTVQLVV
                     WKVTDISPLFLFSGIVLYFLGRDRVRTVGEALLYFGTIFYGLSLIGDAAAPLKDSPVF
                     GRYFLSAQNPLLALLLGLVFTGIVQASAIPISMLVMLGMQGLMSIDNAVPIVIGANVG
                     TTVTALIGSLASDVNGRRTALAHLIFKCSGALVCMLFLSPFVTVLKAMSSSIAQQVAL
                     CHFLFNIVIVFIFMFLLTPFSNAIRRIIPGEHAVIAMWPEYLNTKHLGNPDMALVCVR
                     NELKRQIALAQRMLLESLELFVAYRRNKRQNVMYMELVMDNLQTEITTYLWGISCGAL
                     SQPLTKKLFAFSSFAYDIERMGDRSMNLGELAEQKFRRKAHFTPAANEELRDIGTLVL
                     DNLADAGRLIEKKDIALVRLIVEREHRIDVKIRNATGKHLERFYKKLCLAEAGPIYVD
                     VLVNLEHISNHCLVIAQLTNELEEEETV"
     gene            complement(3123..3452)
                     /gene="metG"
                     /locus_tag="PKJ17_01860"
     CDS             complement(3123..3452)
                     /gene="metG"
                     /locus_tag="PKJ17_01860"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOD74741.1"
                     /translation="MENITFDDFIKIDLRVAEIRACEDIEGADKLYKLTIDMGEERLI
                     VAGIKQYYTKEELVGKKIAVVANLEPRKLRGIMSHGMLLAASSEDKSSVVLLTPDKAI
                     PNGSKIS"
     gene            complement(3505..4131)
                     /locus_tag="PKJ17_01865"
     CDS             complement(3505..4131)
                     /locus_tag="PKJ17_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOD74742.1"
                     /translation="MGISFNPSKVVAVGLNYRDHARELKMDLPDYPLIFMKPSTSVVG
                     DGDPIVFPAQTQELHYEGELGIVIGKRARNVPVGEARDFIAGYTCANDVTARDLQRMD
                     GQWTRAKSFDTFCPLGPRIVRDIDPTSLEIVTRVNGGVKQRSTTANMVFDVYDLVSFI
                     SGIMTLLPGDVIITGTPPGVGSLLPGDTVEVEIEGIGTLTNTVTAAVP"
     gene            4267..>5612
                     /locus_tag="PKJ17_01870"
     CDS             4267..>5612
                     /locus_tag="PKJ17_01870"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOD74743.1"
                     /translation="MSITSFRTLFYHVLLLAVVALAAAGCAINPVTGEQELMLVSERQ
                     EIQLGNELYPNALWSGEGGGGEFRDPALKSYLSSLVVNIHRVSHRPNLPVDFAVQNTS
                     VPNAWAIPGHVVMTRGLLAALDNEAEFVYVMGHEMGHVSARHSARQMTYGMLGQFLLA
                     GAAVAMAGSEYSDAALTLGSAGAGLILLKFSRNDELEADRLGVEYMTRLGYDPRAALS
                     AHRNLEKVSDQYARSIGQSRQESSFFSELLSSHPRTSIRVEEIQNIINQTPRAAIAGD
                     GTARQRFQGAVANIRRVNAVYVNYYDKAVRSLEKNNTGEALSFVRQAIAQDPGQAPFY
                     ALYGSILVKQNNLPEAERYFSKALSIDGNYQPALRGMGAIRYAGNRYSESVQYLERSL
                     KLFPQDPASNYFMGMNYYRTRSYRYAIGYLKPFSEAAPKHPRIHAILGQCYEAVGD"
BASE COUNT         1134 a         1608 c         1674 g         1196 t
ORIGIN      
        1 gagcttgtag gcgcttctcg cccggaaccc ttcctgcttc gccttcttgt agaatgggtc
       61 tttgaggatg aacctagcca taggggttga ttatgagatc gctttcggaa gggccttcct
      121 gcccggtctc caggctcttc ctcaaaaaga aggagagggc atggtcgaag gctgttttgt
      181 cccgaaggat cgagagcgtg cccttgtatc gcgccccatc gcccatgcag tgaaagagat
      241 aggcatagtc ctcgaggagg cgtctgaaaa gaggagccag cgacgcgata tcctggcgtc
      301 ccacgaggtc cctgaggtag gaatccttct tttcttccat catgtgccgg ggcaggacga
      361 tcgtggaccc tccagtcgag aggtaggacg cgcggtcccc ttcaatgcct tcccaggaga
      421 gggaaaaagg cgtgaagatc tcgtgcctga gaacttcgtg gagcggctgg ggagcgacgt
      481 cgtcggccac ggggagggag tggatgtcat ccggcttgtg gacggtgtct ttcagctggg
      541 cgacaaagga cgcgagggac tgtatggcat cggtgaaacg gccggagagt ctcgcccctt
      601 cctcgacgat gaatgccgcg taggaggggg atatctcaac caggaaagcg ggctctttcg
      661 tgccgctcct gtaggcggca acgatctcgt tgagcgacct tctgtccacc ggcgaactca
      721 tgagttcgcg caggccctcg cggaaatgca gatcgccgtt gaggaagacg aaggtattct
      781 tcctgacctc gtacgcggcc atgaccattc gggagccggc atggtcaaag ttcgtcagga
      841 agcccatgtt ggtccgcgac tcttccgcct tcctgaggac gggctcgcct tccgggcgga
      901 gatcttccac ctttacgccc acggacctca ggcggaatat ggtctttctg atgagcttgc
      961 ggatctcctt atccgtctcc tggagataga tggcattaag aagaacgccg gtctcctcgt
     1021 tcctttcctt tcccaggccc tcgagcaccg aaaccctttc gtcgcccgtg agggtgaagt
     1081 agtactccac ttttttttcg agatcctggg cgctgtcgat cgccctgacc aattcatcca
     1141 tgatccgccg tgtccttttc tctcgatctc aatgcttgac ggagaacccc gccttctcga
     1201 cggccgcttc gaggtccttc tggctcgcct tgccctcgtc atacctgacc acggcgcttc
     1261 cgatgtccac atcactcgag tcgaccccgg cgacagcgcc gagggccttt ttgaccgcca
     1321 taacacaatg ctggcagctc atgccgtcta ttttaagttt cgcctcagcc atcgtgtcct
     1381 cctttttgca tccattataa cacaaaagac ggcttgaatc gcttgagcgg cttgaatcgc
     1441 ttgagggcta aaagaaagaa ccttacagtt ttgtcgctct cagctattca agcgattcga
     1501 gccgctcaaa ctgtctcttc ctcttccaac tcgttcgtga gctgggcgat caccaggcag
     1561 tggttcgata tgtgctccag gttgacaagg acatccacat atatgggtcc ggcctcggcg
     1621 agacagagct ttttgtagaa cctctccagg tgcttgcccg tggcgttcct gatctttacg
     1681 tctatcctgt gttcccgttc gacgatgagg cgtaccaacg cgatatcctt cttctcgatg
     1741 agccggccgg cgtccgccag gttgtcgagc accagggtgc ctatgtcccg aagctcctcg
     1801 ttggcggccg gtgtgaaatg ggctttgcgc ctgaacttct gttccgccag ttcaccgagg
     1861 ttcatggagc ggtctcccat gcgttcgatg tcataggcaa atgaggaaaa ggcgaagagc
     1921 ttcttcgtga ggggctgtga aagggcgccg caggagatgc cccagaggta tgtcgtgatc
     1981 tcggtctgga ggttgtccat gacgagttcc atgtacatga cgttctgtcg cttgttcctc
     2041 ctgtatgcga cgaaaagttc gagggattcc aggagcatac gctgtgccag cgcgatctgc
     2101 cgtttcagct cgttcctgac gcagacaagg gccatgtcgg ggttgcccag gtgtttcgtg
     2161 ttcaggtatt ccggccacat ggcaatgacg gcgtgctccc cggggatgat cctgcggatc
     2221 gcgttcgaaa aaggggtgag gagaaacatg aagatgaaga cgatgacgat attgaaaaga
     2281 aaatggcaga gggccacctg ctgggcaatg ctcgacgaca tcgcctttaa cacagtgacg
     2341 aagggcgaca ggaaaagcat gcacaccagg gcgccggaac acttgaagat gaggtgagcc
     2401 agcgctgtcc gtctgccgtt gacatcgctc gcgaggctgc ctatgagggc cgtcaccgtg
     2461 gtgccgacgt tcgctccgat gactatgggg accgcgttgt ctattgacat gagcccctgc
     2521 ataccgagca tgacgagcat gctgattggg atggccgacg cctgtacgat ccccgtgaag
     2581 acgagaccca ggaggagagc cagcaggggg ttctgcgcgg aaaggaaata gcggccgaag
     2641 acgggactgt ccttgagcgg ggccgctgcg tccccaatga ggctcaggcc atagaatatg
     2701 gtgccgaaat agagaagcgc ctcaccgacg gtcctcacgc ggtccctgcc gagaaagtac
     2761 agcacgatcc cgctgaagag gaagaggggg gatatgtccg tcaccttcca cacgacgagc
     2821 tgtacggtga gcgttgtccc gatgtcggcg ccgaggatga tccccagtga gtgatagaag
     2881 ctgacgagcc cggcgctgac gagccccatg gtgagcagcg tcgtcgccga gctgctctga
     2941 aagaagatcg tggtcacgaa ccccgtgatc agcccgtaga acggcttccg gacggaaaaa
     3001 cggatatact cccgtatccg ggagctgaat acccgctgca tctgctcgct aagcttgagc
     3061 atgcccagga ggaaaagggc cagacctgcc agaaaaagaa aaacgtaatg gatcatccca
     3121 tgtcaggata tcttgctgcc gttaggtatg gccttgtcgg gcgtgaggag cacgacggag
     3181 gacttatctt cgctggaggc ggcaaggagc ataccgtggg acatgatgcc ccgaagcttg
     3241 cggggctcca gattggccac aacggcgatc ttcttcccca cgagctcttc cttcgtgtag
     3301 tactgcttga taccggcgac gatcagacgt tcttcgccca tgtcgatggt cagcttgtag
     3361 agcttgtccg ccccctcgat atcttcgcag gcccgtatct cggccaccct caggtcgatc
     3421 ttgatgaaat cgtcaaacgt gatattctcc atagctgctc cttgggttca atgacgccgg
     3481 tctcagtccc ggcggtcagg gtagtcacgg cacggcggcc gtgaccgtgt tcgtaagggt
     3541 ccctatgccc tcgatctcga cctcgacggt gtcgccgggg agaagagagc ccaccccggg
     3601 aggcgttccg gtaatgatga cgtcgccggg taaaagggtc attatccccg agatgaagga
     3661 cacaaggtca tagacgtcga agaccatgtt ggctgttgtc gaccgctgct tcaccccacc
     3721 gttgacgcgc gtcacgatct cgagggaggt cgggtctatg tccctgacga tccgcggtcc
     3781 caggggacag aatgtgtcga aggacttggc ccgtgtccat tgtccgtcca tccgctgcag
     3841 gtcccttgcg gtcacgtcgt tggcgcaggt gtacccggca atgaaatcgc gggcttcacc
     3901 gacgggaaca tttcgcgcgc gtttgccgat gacgattccg agttctcctt cgtaatggag
     3961 ctcctgcgtc tgcgcgggga aaacgatggg gtctccgtca ccgacgacgg acgtggacgg
     4021 tttcatgaag atgagcggat agtcgggaag gtccatcttc agttccctgg catggtccct
     4081 gtaattgagc cccacggcga cgacctttga agggttgaag cttatgccca tgatgcctcc
     4141 cggatcgtgt ccatgctgtt ctattttacg ctttccccat gttcggctgt caagctattt
     4201 cctttcttga caaaggtccg cggggaacat aagaatgtag ggtagactac ggagaaaggg
     4261 gcggaaatgt caatcacatc gtttcgtacg ttattctatc atgtccttct gctggcggtc
     4321 gttgcgctcg ccgccgccgg ctgtgccatc aatcccgtga cgggcgagca ggaattgatg
     4381 cttgtctccg agagacagga gatacagctc ggcaacgagc tctaccccaa cgccctgtgg
     4441 agcggagaag gcggcggtgg cgaattcagg gacccggcgt tgaaatccta cctgtcgagc
     4501 cttgtcgtga acattcaccg cgtgtcgcac aggcccaacc tccccgtcga ttttgccgtt
     4561 cagaacacct ccgtgcccaa tgcctgggcg ataccgggtc atgtcgtgat gacccggggc
     4621 cttctcgcgg cgctcgacaa tgaggcggag tttgtttacg tcatgggcca cgagatgggg
     4681 catgtttcag cgcgccattc ggcgaggcag atgacgtacg gcatgctcgg ccagttcctt
     4741 ctcgccggtg cggcggttgc catggccggc tccgagtact ccgacgcggc gctgaccctg
     4801 gggtccgcgg gtgccgggct cattcttctc aagttcagca ggaacgacga gctcgaggcg
     4861 gacaggcttg gcgtagagta catgacgagg ctcggctacg acccgcgggc ggccttgagc
     4921 gcccacagga accttgagaa ggtctccgac cagtacgcgc ggtccatcgg gcagagcagg
     4981 caggaaagca gctttttcag cgaactcctg tcttctcatc ccaggacctc catccgtgtc
     5041 gaggagatac agaacatcat caaccagacg ccccgggcgg caatagccgg cgacggcacg
     5101 gcacggcagc gcttccaggg ggccgtggcc aacatccgtc gcgttaacgc ggtctacgtg
     5161 aactactacg acaaggccgt ccgctccctt gagaagaaca ataccggtga ggccctgtcc
     5221 ttcgtcaggc aggccatagc ccaggacccc gggcaggcgc ccttctacgc gctctatggc
     5281 agcatcctgg tgaagcagaa caaccttccc gaggcggagc gctacttttc aaaggcgctc
     5341 agcatcgacg gcaactatca acctgccctg aggggaatgg gtgccatcag gtatgccggt
     5401 aacaggtatt cggagagcgt gcagtacctt gagaggagcc tgaaactctt cccacaggac
     5461 ccggcatcga attatttcat gggaatgaac tactacagga cacgttccta caggtacgcc
     5521 ataggctacc tcaaaccctt ctcggaggcc gcgccgaagc atcccaggat ccacgccatc
     5581 ctgggacagt gctacgaggc agtgggcgac ta
//
LOCUS       DAOPOA010000050         5610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_15837_length_5610_cov_3.956436, whole genome shotgun sequence.
ACCESSION   DAOPOA010000050 DAOPOA010000000
VERSION     DAOPOA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15837_length_5610_cov_3.956436"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PKJ17_01875"
     CDS             <1..510
                     /locus_tag="PKJ17_01875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD74744.1"
                     /translation="VLNCFSYTGGFTVYAVAGGARRVVSVDSSRRALDIAKENIILNG
                     FDTSRCEFVCDDAKRYLREPGRHFEATVLDPPAFIKDRRKKQEGLAGYRAINEMALRN
                     LSPGGVLVTCSCSAHLSLSDFRFLLSEAGAKTGRPLTLLETHTHGIDHPQLVPFTEGE
                     YLKCFILRG"
     gene            complement(620..1384)
                     /gene="hisF"
                     /locus_tag="PKJ17_01880"
     CDS             complement(620..1384)
                     /gene="hisF"
                     /locus_tag="PKJ17_01880"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOD74745.1"
                     /translation="MKTVKIMPCLDMKNGRVVKGVHFVDLKDAGDPVENAAYYQKEGA
                     DELAMLDIAATVENRKTRLEWVKEVSSVITIPLTVGGGINTTDDIELVLKAGADKVSM
                     NSAAVKDPTLVKEAADRFGGGRIVIAVDARRNGAMPSGFELVVAGGTTPVGKDAVEWA
                     KRCQELGAGTILPTSMDGDGTLKGYDLEFTKAISDAVTLPVIASGGAGTLEHFYEGVV
                     RGGADILLAASVFHFRTFTVRQVKEYLKGKGVAVNL"
     gene            1644..3290
                     /locus_tag="PKJ17_01885"
     CDS             1644..3290
                     /locus_tag="PKJ17_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOD74746.1"
                     /translation="MKIEKLRNLGIFYKIMGISAVAIVVIILWNILYLLPQMERIMLK
                     EKQNAVKDVVDVAFSMLNDYDKRVKAHEIEMGMAQMAAIKSIQQLRYRGENYFWINDT
                     QPKMIVNPVMIDLEGQDVSGFEDPKTKKKIFVEMTAIAKDRKAGFYEYNWKRSGATSF
                     STKQSYIKYFEPWGWVIGSGMYLDDVNREIAKFRWIIIGTTLVLALLVLALSYLIANN
                     IRKNLSKAVDASRSLSEGDLSIDISANGSDETGQLLQSMKNMIGSFNTMIGSIKSSVD
                     SLDTSVETIKTLADRTSEGTRTQADQANQISVSAEEMSQTIVDVANNASGVKDASTNA
                     MEAAITGKKVTQEAVAEVNKVQHSSTELANTIQKLNQRVSEVAKIITIIDDIADQTNL
                     LALNAAIEAARAGEHGRGFAVVADEVKKLAEKTMNATKEITNHLTTVQRESEETTRSM
                     EITSAEVERVTEYMQEVEQSLGNIVSSSEQVRDQIMQIATAVEQQSASSEEIARNITS
                     TSGIAKEIEAVSSQVMSEVNKLVEITQVLGSSAAQFKTKA"
     gene            complement(3383..3916)
                     /locus_tag="PKJ17_01890"
     CDS             complement(3383..3916)
                     /locus_tag="PKJ17_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74747.1"
                     /translation="MAEIKSAIELAMEKTKGLVMDDREKRSLALKELAAGMMTIYRRY
                     REGLSGDDETKAQLDALECDSAGKRKIALDILGDEFEAGDDVAQMAPLFSFIGFVVDE
                     KARRELLALQKECLGELEKIRGSIASRITQDLAASGIKGDSVEPNIEAWPVWKKASSD
                     VRRAFRRQIDKWKEGLL"
     gene            complement(3919..4314)
                     /locus_tag="PKJ17_01895"
     CDS             complement(3919..4314)
                     /locus_tag="PKJ17_01895"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOD74748.1"
                     /translation="MDRVIIEHLPENKDLAGAKRWVDEKGEFVQISWREDIGHLAFFE
                     LRKGQFRGGHYHERKEEVFYVVSGTIRAVFADLGSGGRETRILEKGMKVRVGTRVGHR
                     FEGIEDSLVIEYSPQYYDKTDALRADLGE"
     gene            complement(4317..4859)
                     /locus_tag="PKJ17_01900"
     CDS             complement(4317..4859)
                     /locus_tag="PKJ17_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOD74749.1"
                     /translation="MLYEYDGRRPTVGKDSYVSDIAHVIGDVVIGDNCYIGHGAILRG
                     DYGAIVVGSGTAVEEGVVVHAPPDRYCRIGKRVTIGHGAVIHAASIGDSAVIGMGAVL
                     SIESEIGEGTIVAEGTVVRMRQKVTARVVVGGNPMRVIRQIAPKDMEFWDKGKQLYID
                     LAKKYLEKGMRPLQGPPGGA"
     gene            complement(4897..>5610)
                     /locus_tag="PKJ17_01905"
     CDS             complement(4897..>5610)
                     /locus_tag="PKJ17_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOD74750.1"
                     /translation="EAFKKAYPQIPFDSMRPTPVNGLYEVSKGGDIIYFFEEKGLLFV
                     GEIIDGTGKNLTEDRKSEMLVANVKKMPLDKAIKIGNGKQTVIEFTDPDCPYCRRAAS
                     YFESRKDLTRYVFFFPLPSHKDAENKIKYVFCSSDRAKAYEEAMKGKLDTQKYKVCKK
                     QEAADLLNAHKQIGVQIGVSGTPMFIINGKDLVMGANFPAIEAALRTDDQKQGSSEQK
                     TGAPEKKPAEPEKKATEGK"
BASE COUNT         1174 a         1698 c         1523 g         1215 t
ORIGIN      
        1 gtcctcaatt gcttctccta tacgggaggg ttcacggtct acgccgtggc cgggggcgcc
       61 cgccgcgtcg tcagcgtcga ctcttcccgg cgcgccctcg acatcgcgaa ggagaacatc
      121 atcctcaatg gattcgacac gtcacggtgc gaattcgtct gtgacgacgc gaagaggtat
      181 ctcagggagc cgggccggca cttcgaggcg accgtgctgg accctcccgc gttcataaag
      241 gaccgcagga agaaacagga aggccttgcc ggctaccggg cgatcaacga aatggccttg
      301 agaaacctct cgcccggggg cgtcctcgtg acatgctcct gttccgccca tctgtccctg
      361 agcgatttcc gcttcctcct gtcggaggcg ggggcaaaga cgggaagacc gctcacctta
      421 ctcgaaaccc acacccacgg catagaccat ccccagctcg taccgttcac ggagggggaa
      481 tacctgaaat gtttcatcct gaggggatga aacatttccc gttttaagtg attgagtttt
      541 aagtgttaag tcaaaggcag gagcagaaag gatttcatct tttacttaaa acttaaaacc
      601 taaaacttaa aaccgtcttt tacaggttta ccgccactcc cttccccttc aaatactcct
      661 tcacctggcg gaccgtgaag gtgcggaagt ggaagaccga tgcggccagc agaatgtccg
      721 cgccaccacg cacgacacct tcgtagaagt gctccagggt tcccgctccg cccgaggcga
      781 tcacgggcag ggtcaccgcg tcggatatgg ccttcgtgaa ctcgaggtcg tatcctttca
      841 gggtcccgtc gccgtccatg ctcgtcggca ggatggtacc ggcaccgagt tcctggcagc
      901 gcttcgccca ttccacggcg tccttgccga cgggggtcgt accgcccgcc acgacaagct
      961 cgaaacccga gggcattgcc ccgttacgcc tcgcatcgac ggcgatgacg atcctcccgc
     1021 ctccgaacct gtcggcggct tccttcacca aggtcgggtc cttcacggcg gcgctgttca
     1081 tggacacctt gtcggccccg gccttgagga cgagctcgat gtcatccgtc gtgttgatgc
     1141 cgcctcccac agtgagggga atggtgatca ccgaggagac ctccttgacc cattccagcc
     1201 gggtcttgcg gttttcgacg gtggccgcaa tgtcgagcat cgcgagttca tccgcccctt
     1261 ccttctggta ataagccgca ttctcgacgg gatctccggc atccttgagg tcaacaaaat
     1321 ggacaccttt cacaacgcgt ccatttttca tatcgagaca tggcatgatc ttcacggtct
     1381 tcattgtcag gctcctcatc ttttggattt ccgtccggcc tccccggccg gcggcggttc
     1441 ataaatacat attcgctttc ttcatcccct gtcaaggagc cttcttccgc atctccggga
     1501 aatgccttgc gagcccctgt aaattcttgt aaagttatct gacgggcacc cgataatatc
     1561 gttaaaggca tatttcacca ccttgcgtcg atcagaaggc ctctgccata cgcattgaaa
     1621 aatgaaccgg gaggtgtgtt cacgtgaaga tcgaaaagct caggaacctc ggcattttct
     1681 acaagatcat ggggatctcg gccgttgcca tcgtcgtcat catcctctgg aacatcctgt
     1741 atcttctccc tcaaatggag cggatcatgc tgaaggagaa acagaacgcc gtcaaggacg
     1801 tcgtcgatgt cgcgttcagc atgctcaacg attatgacaa gagggtgaag gctcacgaga
     1861 tcgagatggg catggcccag atggcggcga taaagtcgat ccagcagctt cgttatcgcg
     1921 gcgagaatta tttctggata aacgacacgc agcccaagat gatcgtgaac cccgtcatga
     1981 tagacctgga aggacaggac gtgtcgggtt ttgaggaccc caaaacgaaa aagaagatct
     2041 tcgtcgagat gaccgccata gcgaaggata gaaaggcggg cttctatgaa tacaactgga
     2101 agaggtcggg ggcgacgtcc ttctccacga agcaatccta cataaaatac ttcgaaccct
     2161 gggggtgggt gataggttcg ggcatgtatc tcgacgacgt gaacagggag atcgcgaaat
     2221 ttcgctggat catcatcggc accactctcg ttctagccct tctcgtgctc gcgttgagct
     2281 acctcatcgc caacaacata cgaaagaacc tcagcaaggc cgtcgatgcc agcagatccc
     2341 tgtcggaagg cgatctcagc atcgacatca gcgccaacgg ttcggacgaa acgggacagc
     2401 ttctgcagtc gatgaagaac atgataggct ccttcaacac gatgatcggc agcatcaaat
     2461 catccgtcga cagcctcgat acgtcggtcg agaccatcaa gacactcgcc gacaggacgt
     2521 cggagggaac acgaacacag gccgatcagg ccaaccagat atcggtctcg gccgaagaga
     2581 tgagccagac catcgttgat gtcgcaaaca acgccagcgg cgtcaaagac gcctcgacga
     2641 acgcgatgga ggccgcgata acggggaaga aggtcactca ggaggccgtc gccgaggtca
     2701 acaaggtcca gcattcctcc accgaactgg caaacacgat ccagaaactc aaccagcgcg
     2761 tcagcgaggt tgccaagatc ataaccataa tagacgacat agccgaccag acgaacctac
     2821 tggcgctcaa cgcagccata gaggctgcgc gcgcaggaga gcacggacgg ggctttgccg
     2881 tcgtcgccga cgaggtgaag aagcttgccg aaaagacgat gaacgcgaca aaggagatca
     2941 cgaaccatct gaccacggtt caaagggaat cggaggaaac cacgaggtcc atggagatca
     3001 cctcagccga ggttgaaagg gtgacggagt acatgcagga ggtcgagcag tcgctgggga
     3061 atatcgtctc gtcctccgaa caggttcgcg accagatcat gcagatcgcc accgccgtcg
     3121 agcagcagtc ggcgagttcc gaagagatag cgaggaacat cacctccacc tccggcatcg
     3181 ccaaggagat cgaggcggtg tccagtcagg tgatgtccga ggtcaacaaa ctcgtcgaga
     3241 taacgcaggt actgggaagc tccgcggccc agttcaagac aaaagcctga aggcggcttt
     3301 aagggggtta cagggtaacg gggtgagccg ctggaaggga tgcgggcata cgggctttct
     3361 tccgtgtgcc ccttcttttg gcttacagaa ggccttcctt ccatttgtct atctgccgcc
     3421 tgaaggcgcg gcggacatcc gaggacgcct tcttccatac cggccacgcc tcgatattgg
     3481 gctccacgct gtctcccttg atgcccgacg cggcaaggtc ttgggttatc cgggatgcga
     3541 tcgagcctcg tatcttttcg agttccccga ggcactcctt ttgaagggcc agcaattccc
     3601 tgcgggcctt ctcatcgacg acgaaaccga tgaacgagaa gaggggggcc atttgggcaa
     3661 catcgtcccc agcctcgaac tcgtcgccaa ggatgtcaag ggcgatcttt ctcttcccgg
     3721 cactgtcgca ttcaagcgcg tcgagttggg cttttgtctc gtcatcgccg gagagaccct
     3781 cacgataacg ccggtagatc gtcatcatcc cggccgcaag ctccttgagg gcaagggacc
     3841 ttttttccct gtcgtccatg acgagaccct tcgtcttctc catggccaat tcgatggcac
     3901 tctttatctc cgccatgatc actctcccag gtctgccctc agggcgtcgg tcttgtcgta
     3961 gtactgaggg ctgtactcga tcacgaggga atcctcgatg ccttcgaagc gatggcctac
     4021 acgggtcccg acacggacct tcatcccctt ttcaaggatg cgggtctctc tcccgccgct
     4081 cccgagatcg gcgaagacgg ccctgatcgt gccgctgacg acatagaaga cctcctcttt
     4141 gcgctcgtgg taatgaccgc cgcgaaactg tcccttgcgg agttcgaaaa aggccaggtg
     4201 gcctatgtcc tccctccagg atatctgtac gaactcgcct ttctcatcaa cccagcgctt
     4261 ggcgccggcg aggtccttgt tctcggggag gtgttcgatg atcaccctgt ccattgtcat
     4321 gccccgcccg gcggtccctg cagcggtctc atcccttttt ccaggtactt cttcgccagg
     4381 tcgatgtaga gctgtttgcc cttatcccag aactccatgt ccttcggggc gatctgtctg
     4441 atcacgcgca tgggattccc gcctacgacg acgcgcgccg tcaccttctg ccgcatcctg
     4501 acaacggtcc cctcggcaac gatggtgccc tcaccgatct cgctctctat actgaggacc
     4561 gctcccatgc cgataacggc cgaatcaccg atgctcgcgg cgtggatgac ggctccgtgg
     4621 ccgatggtga ccctctttcc gatacggcag tacctgtcgg ggggcgcgtg gacgacgacc
     4681 ccctcttcca ccgccgtgcc gcttcccacg acgatggcgc catagtctcc cctgagaatg
     4741 gctccgtggc ctatgtaaca gttgtcacct atcacgacat cgccgatcac atgggcgatg
     4801 tcgcttacat aactgtcctt gccgaccgtc ggccttcgtc cgtcgtattc gtaaagcatg
     4861 cgtcgttccg ccttccgttc accccgggga agaggctcac ttgccctcgg tcgctttctt
     4921 ttcgggttcg gccggtttct tttccggcgc gcccgttttc tgctccgacg agccctgttt
     4981 ctggtcgtcc gtcctgaggg cagcctctat cgcgggaaaa ttggcaccca tcacaaggtc
     5041 tttgccgttt atgatgaaca tcggggtgcc gctcactccg atctggaccc ctatctgctt
     5101 gtgggcgttc aacagatccg ctgcctcctg ctttttgcac accttgtatt tctgcgtatc
     5161 cagcttgccc ttcatcgcct cttcgtatgc ctttgccctg tcggaggaac agaagacata
     5221 cttgatcttg ttctcggcgt ccttgtggga aggcaggggg aagaagaaaa cataccgcgt
     5281 caggtccttc ctggactcga aatatgacgc ggcccggcgg cagtagggac aatcggggtc
     5341 ggtgaactct atgaccgtct gcttcccgtt gccgatcttg atcgccttgt cgaggggcat
     5401 cttctttacg ttggccacga gcatttccga cttcctgtcc tccgtcaggt tcttgcccgt
     5461 cccatcgatg atctcgccga cgaagagaag gcccttttcc tcaaagaaat agatgatgtc
     5521 accacccttc gagacctcat agaggccgtt gacgggtgtc gggcgcatgg aatcgaaggg
     5581 tatctgcgga taggccttct tgaatgcctc
//
LOCUS       DAOPOA010000051         5593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_15887_length_5593_cov_3.420549, whole genome shotgun sequence.
ACCESSION   DAOPOA010000051 DAOPOA010000000
VERSION     DAOPOA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15887_length_5593_cov_3.420549"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PKJ17_01910"
     CDS             <1..259
                     /locus_tag="PKJ17_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOD74751.1"
                     /translation="YFTYRQAGISVPLTAEAQGRYGAEISRGEAQPGDLVLFRIRNDD
                     HIGIMVNGSDFVHASKSRGVAIDSLESTYWRRYLIGFRKLI"
     gene            687..2003
                     /gene="dnaA"
                     /locus_tag="PKJ17_01915"
     CDS             687..2003
                     /gene="dnaA"
                     /locus_tag="PKJ17_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOD74752.1"
                     /translation="MSDILSEIKPKIASIVSEDSFKTWIEPLSFIDLTNQNCRIGVPN
                     AFFRDWVSENFEPIIVTILKDATKEDVRIEYVLKRDEKAEEKKKVVLRKQTTFSQFNP
                     KYTFENFVVGTSNQFANAACLAVSTNPGKTYNPLFIYGGVGLGKTHLLNAVGNFLVRH
                     GTINPDRICYITAETFTNELINSIRYEKMDDFRNRFRKMDIILIDDIQFIAGKERTQA
                     EFFHTFNTLYDNMKQIVVTSDKFPRDIENFEERLKSRFEWGLVADIQSPDIETKVAIL
                     NKKAEAEKIVLPLDVAFFIANNSEDSIRSLEGTLIRVGAYASLNDTPITTDLVKQIMS
                     HIIKDKNKEITIDMILKEVCSHFSVTLGDIRSSKRIKSIMVPRQVAMYLSRKMTDSSL
                     VSIGEKLGGKDHATVLHSIKKIDSELNVKKELKSTVEKIVQRLKST"
     gene            2121..3206
                     /gene="dnaN"
                     /locus_tag="PKJ17_01920"
     CDS             2121..3206
                     /gene="dnaN"
                     /locus_tag="PKJ17_01920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOD74753.1"
                     /translation="MKIILDKNIILQPISKIVSITEKRSLMPILSNILIDFGKEGTTV
                     YSTDLEVSAITSIDFVTDMERKVVVHGRKFLEILKELDSGEIEFIFEDNVMTIRQKMT
                     EISLSLQDPEEFPETREISGRDEFTIPGSILLEMIDKVEFAVSIDETRYILTGMFMKG
                     QDDKVVVVGTDGFRMALYQKEIDGLKPFQGMTIPKRSISEVERVIGEDEDVRVVIDEK
                     HVQFSTEKMKIISRIIEGNFPDYENVLPSGNTNVAEVERELLFKGLKKVSSIIGRSEP
                     VKMTLSENTMTIEAESDIGRAKEVIGITYDGEETTMNFNVKFLIDVVTHLTGDQVTIA
                     APKAYGAVLFRSGDLDGYKNIVMPIRI"
     gene            3210..5570
                     /gene="gyrB"
                     /locus_tag="PKJ17_01925"
     CDS             3210..5570
                     /gene="gyrB"
                     /locus_tag="PKJ17_01925"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOD74754.1"
                     /translation="MGEYGAESIKILGGLDAVRKVPSMYIGNTNIDGLHHLIYELVDN
                     SVDEALEGYCNRIAVTIHRDNSVTVEDNGRGIPVTMHSEENMSALEVVLTKLHAGGKF
                     DKDTYRYSAGLHGVGLSVVNALSHYLEVEVKRAGQVYFQRYEQGNRMTDLTVIGDTES
                     TGTKVKFKADATIFETTEYSYDTLAHRMREISFLNNGIHIILNDERKARKQDFKHEGG
                     IRSFVKYLNASKTVLFDEPIYVSNSRESLDLLEVALQYNDGYNENIQSFVNNVNTKEG
                     GTHVAGFRSALTRCVNNFIQSNMQGKVKENLTGDDIKEGLVAVVNIKVQNPQFEGQTK
                     AKLGNSEIKGIVESLLNEKIGEYLELNPQIARTIVGKALEARRAREAAKKAKELVKSK
                     SLIENGVLPGKLADCQESDPVQCELFIVEGDSAGGSAKQGRNRKTQAILPLRGKILNV
                     EKSRQEKVLTNQEIRSIYLAVGIGAEGKGRLRYHKVIIMTDADVDGSHIRTLLLTFFY
                     RQMPDLITDGHLFIAQPPLYKIKSGGKESYAKDEDEFERAITQRGMEKTRCAVNGDTQ
                     EGPELAEKVEKIKTVEGFLRNSLVDGIDREISLGLMRFDIVKREDFQKEDKLGRLLES
                     LRGSGYRCTMVWDREHNLFCIEAEAKGSKGRMIRVEYEMCAVSDYADAYKAYVEVRNF
                     YEEGVRVTDGNKPPVSFSPDEFLRHILEKGKEGVNIQRYKGLGEMNAEQLWETTMNPE
                     KRRVVKVTIEDAVAADQVFTVLMGNNIETRRTFIEENALSVRNLDI"
BASE COUNT         1541 a         1413 c         1552 g         1087 t
ORIGIN      
        1 ctatttcacc tacaggcagg cgggcatctc tgtgccgctc acggccgagg cacagggacg
       61 ctatggcgcc gagatatccc ggggggaggc gcaaccgggc gacctcgttc tcttcaggat
      121 caggaacgac gaccacatcg ggatcatggt caacggctcc gattttgtcc atgcctccaa
      181 atcacgcggt gtggccatcg actccctgga atccacctac tggcgaagat acctgatagg
      241 gttcagaaaa ctgatatagg cgtaaggcgt agcccggtat ctgtatgatc ttctttcccc
      301 tgtcatcgag gaggacggcg tgaagaagta cacgagaacg ggtttcttct ccttcgccgc
      361 gagtttccgg acctccccca tggtctcctg gcctgtatcc gcaagggggg ggaggaggga
      421 gaaaaggagg aggatcaaag ccaggatatt tcgtcccggc atgctccatg gtatcagaaa
      481 acgatggagc ccgcaaaccg gtgcgggtgc gccggaaggg ccgaggaaaa actattgact
      541 tgtagaaaaa tgtcctgtag aattggatac acgttacccg gcacattact ggacaagatt
      601 ctcagttaca ttagggttaa ttccaagcct ctggggtact actagttttc cacatatgtt
      661 gaaaagttgt tgaaacaatc catgccatgt ctgatatcct ttccgaaata aagccgaaga
      721 tagcttccat agtcagcgag gacagcttca aaacctggat agaaccgctc agcttcatcg
      781 acctcacgaa ccagaactgc cgcatcggcg tgcccaatgc cttcttccgt gattgggtgt
      841 cggagaactt cgaacccatt atcgtaacca tcctcaagga tgccacgaaa gaagatgtca
      901 ggatcgaata tgtgctcaag agggatgaga aggcggagga aaagaagaag gtcgtcctta
      961 ggaagcagac gaccttcagt cagttcaatc cgaagtatac cttcgagaac ttcgttgtgg
     1021 gcacgagcaa tcaatttgcc aatgctgcgt gtctcgccgt gtcgaccaac cccggcaaga
     1081 catacaaccc tctgttcata tacggcggcg tcggactcgg caagacccat ctgctcaatg
     1141 cggtaggcaa cttcctcgtg agacacggca ccatcaaccc cgatcgcata tgctacatca
     1201 cggcggaaac ctttacgaac gaactcatca actccatacg ctacgagaag atggacgact
     1261 tccgtaacag gttcaggaaa atggacatca ttctcatcga cgatatacag ttcatcgccg
     1321 gcaaggagag gacccaggcc gagttctttc acacttttaa tacactctat gacaacatga
     1381 aacagattgt tgtcaccagt gacaaattcc ccagggatat cgagaacttc gaggagcgcc
     1441 tcaaatcccg tttcgagtgg gggcttgttg ccgacataca gtccccggac atagagacaa
     1501 aggttgccat tctgaacaaa aaggccgaag cggagaaaat tgttctccct ctcgacgtgg
     1561 cattcttcat agcgaacaac tcggaggatt ccatccgctc cctcgagggc accctgatcc
     1621 gtgtcggggc ctacgcgtcc ctcaacgaca caccgatcac cacggacctc gtgaaacaga
     1681 tcatgagcca tataatcaag gacaagaaca aggagatcac catcgacatg atcctcaagg
     1741 aggtatgttc acacttctcc gttaccctcg gcgatataag atcgagcaag cggatcaagt
     1801 ccatcatggt gccccgtcaa gtcgcaatgt acctgtcgag gaagatgacc gactcttcgc
     1861 tcgtaagcat cggcgagaag cttggcggta aggaccatgc cacggtgctc cattccataa
     1921 agaagataga tagtgaacta aatgttaaaa aggagttgaa aagtactgtt gaaaagattg
     1981 ttcaaagatt gaagtcaaca tgattcaaca gttgtcttag ttttttgcac agcgtccatt
     2041 ttcatttaat cctgtgaagt cagttggttg catatttttc aacattttaa caccacttat
     2101 ctgttatgag gagttaattt atgaaaataa tactagataa gaacataatc ctgcagccaa
     2161 tatcgaagat cgtttccata acggaaaaaa gatctctcat gccgattctt tccaacattc
     2221 tcatcgattt tggcaaggaa gggaccaccg tctactccac ggacctggaa gtgagcgcca
     2281 tcaccagtat agatttcgtc acggacatgg agagaaaggt cgttgtccat ggaagaaagt
     2341 tcctggagat actcaaggaa ctggacagtg gagagataga gttcattttt gaagacaatg
     2401 tcatgacgat acgccagaag atgacggaaa tatccctaag tcttcaggac cccgaggagt
     2461 tcccggaaac cagggagata tcgggcaggg atgagttcac catccccgga agcatcctcc
     2521 ttgagatgat agacaaagtc gagttcgccg tttccataga tgagacccgc tatatactca
     2581 ccgggatgtt tatgaaaggc caggacgaca aggtggtggt cgtcggaacg gacggattcc
     2641 ggatggcact ttaccagaaa gagatcgacg gccttaagcc ctttcagggg atgacgatcc
     2701 cgaagaggtc catttcggaa gtggagaggg tcataggaga ggatgaggac gtccgtgtcg
     2761 tcatcgatga aaaacacgtc cagttcagca cggagaagat gaagatcatc tcccgcatca
     2821 tcgaggggaa ttttccggat tatgagaacg tcctcccctc gggcaacaca aatgtcgcgg
     2881 aggtggaaag agagttgctc ttcaaggggc taaagaaggt ctcttccatc attggaaggt
     2941 ctgagccggt gaagatgaca ctgtccgaga atacgatgac gatcgaggcg gagtcggata
     3001 tcggcagggc caaagaggtc atcggaataa catacgatgg tgaagagacg acaatgaact
     3061 tcaacgtaaa gttcctgata gacgtcgtaa cacatctgac gggtgaccag gtgacaatag
     3121 ccgcgccgaa ggcgtatggc gccgtcctct tcaggtccgg ggatctggac ggctacaaga
     3181 acatagtgat gcctataagg atatgagaaa tgggtgaata cggagcggaa agcataaaaa
     3241 tacttggcgg tctcgacgcc gtcaggaaag taccgtcgat gtacatcggc aatacaaaca
     3301 tcgacgggct gcaccacctt atctacgagc ttgtcgacaa cagcgtggac gaagcgctgg
     3361 agggatactg caacaggatc gcggtgacca tacaccgcga caacagcgtg accgtggagg
     3421 acaacggcag gggtataccc gtgacgatgc acagcgagga gaacatgtcg gccctcgagg
     3481 tcgtccttac aaaactgcac gcgggcggga agttcgataa ggacacctac cggtattcag
     3541 caggccttca cggggtaggt ctttccgtcg tcaacgcgct ctcgcattac cttgaggtag
     3601 aggtgaagag ggcggggcag gtctatttcc agcgctacga acagggtaac aggatgacgg
     3661 acctcaccgt cataggagac accgagagca ccggcacgaa ggtgaagttc aaggccgacg
     3721 ccaccatatt cgagaccacg gaatacagct acgatacgct ggcacaccgg atgcgggaga
     3781 tctccttcct caacaacggt attcacatca tccttaatga tgagagaaag gccagaaagc
     3841 aggatttcaa gcacgaggga ggcatccgct ccttcgtcaa gtaccttaat gcgagcaaga
     3901 ccgtgctctt cgacgaaccg atatatgtct cgaactcgcg ggaatccctc gatcttctcg
     3961 aggttgccct ccagtacaac gacgggtaca acgagaatat ccagagcttc gtgaacaacg
     4021 tcaacaccaa agaaggcggc acccatgtgg cgggcttccg cagcgccctg acacgatgcg
     4081 tgaacaactt catccagagt aacatgcagg gcaaggtcaa ggagaacctc accggcgatg
     4141 atatcaagga aggtcttgtg gccgtcgtga acatcaaggt ccagaacccc cagttcgagg
     4201 ggcagacgaa ggcgaagctc ggcaacagcg agataaaggg catcgtcgaa tccctcctga
     4261 acgagaaaat aggggaatac ctggaactca atccccagat agccaggacg atagtcggca
     4321 aggccctcga ggcgcgccgg gcccgggaag cggcaaaaaa ggcgaaggag ctcgtcaaga
     4381 gcaagagcct tatagagaac ggggtgctgc cgggaaaact tgcggactgc caggaaagcg
     4441 acccggtgca gtgcgagctt ttcattgtcg agggcgattc agcaggcggt tcggcgaaac
     4501 agggcaggaa caggaagaca caggccatac tacccttgag agggaagata ctcaacgtcg
     4561 agaaatcgcg gcaggaaaag gtgttgacga atcaggagat acgctccatc tatctcgccg
     4621 tcggcattgg ggccgaggga aaaggacggc tgcgctacca caaggtgatc atcatgaccg
     4681 acgccgacgt cgacggttcc catatacgca cgctcctgct caccttcttc taccgccaga
     4741 tgcccgatct catcacggac gggcatcttt tcatcgccca gccgcccctg tacaagataa
     4801 agtccggcgg taaggaatcc tacgcgaagg acgaagatga attcgagcgc gccatcacgc
     4861 agaggggcat ggaaaagacc cgctgtgccg ttaacggcga cacgcaggag ggaccggagc
     4921 tcgccgaaaa ggtggaaaag ataaagacgg tcgagggctt tctgcgcaat tccctcgtgg
     4981 acgggattga cagggagata tccctcggtc tcatgcgctt cgatatagtg aaacgggagg
     5041 actttcagaa ggaggacaag ctcggcaggc tgctcgagag cttgaggggt tcgggctaca
     5101 ggtgcacgat ggtctgggac cgggagcaca acctcttctg catcgaggcg gaggcgaagg
     5161 gcagcaaagg aagaatgatc cgtgtggagt acgagatgtg tgccgtgagc gactatgccg
     5221 acgcatacaa ggcgtacgtg gaggtgagga acttctacga ggaaggcgtg cgcgtcaccg
     5281 acgggaacaa gcctcccgtc tccttcagcc ccgacgagtt cctcaggcac atactcgaga
     5341 agggaaagga aggggtcaac atccagaggt acaaaggtct cggcgagatg aacgccgagc
     5401 agctctggga gaccacgatg aaccccgaga agagaagggt cgtcaaggtc accatcgaag
     5461 acgccgtcgc cgcggatcag gtcttcaccg tcctcatggg gaacaacatc gagacgcgca
     5521 ggaccttcat cgaggagaac gccctgagcg tgagaaacct cgatatatga taacgatcgg
     5581 cataacgggg atc
//
LOCUS       DAOPOA010000052         5552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_16031_length_5552_cov_3.326542, whole genome shotgun sequence.
ACCESSION   DAOPOA010000052 DAOPOA010000000
VERSION     DAOPOA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16031_length_5552_cov_3.326542"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PKJ17_01930"
     CDS             complement(<1..998)
                     /locus_tag="PKJ17_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOD74755.1"
                     /translation="MKLLKKIPLKFNIYLLKELTGILLLSLGILTFILVLSRLGKIAD
                     LVINKGVGFTDIFALVAFSVPPYLTFTLPMAFLLSVIVVLGRLSTENEILALKASGVN
                     LMWLFVPITLVGLIVTSCGLFNTNVLLPRCSGLFRETLINVIKKGISVDDKEGVFNDS
                     VPGVVIYINKVDNTRRTLTGIVVSDDRDKNVKQTISASKGYVHIDSETFQLYFVLQDG
                     TLHRWEKATDTYRSVSFQDYTFTMNLSQMVQTGGVSRKLAYEMDRNELKKALTTAANE
                     DQRYDLLLEIYKKISLPLSSLAFIILTIPLGVRRKVEGRFSGTLYSLLLFIFYYVL"
     gene            1164..1646
                     /locus_tag="PKJ17_01935"
     CDS             1164..1646
                     /locus_tag="PKJ17_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD74756.1"
                     /translation="MKIAVISDTHLAHPTEGFKRAMKALFSDVDMMLHAGDMTSSSVF
                     DYLSNWDLRAVRGNMDDFDLGARLPESRVEEAAGRRIGIIHGWGSPQGLEDKVLRVFR
                     DVDIIVFGHSHIPFNMKKGGAILFNPGSCRGGYSVSGTVGIIEIGAEVTLRHLKVDEL
                     "
     gene            1677..>3804
                     /locus_tag="PKJ17_01940"
     CDS             1677..>3804
                     /locus_tag="PKJ17_01940"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOD74757.1"
                     /translation="MDGICISFLWHMHQPYYKNTFTGEYLLPWTLLHGTKDYYDMGAM
                     LKGFPGMKQNFNFVPSLLEQLVDYEDLDVKDTYLDVFRKDPSHLTEKNKVFLLGNFFN
                     ANWENMIRPFPRYYELLAKRGLYCPADGLSKVTGYFSEDEFRDLQILFFLAWTDPSFF
                     ADFEGLRYLREKGKKFSEEDKKVLMDVQKNILKGTIPLYRELAAEGSVELSTSAFYHP
                     IIPLLIDSASAREAMPDVNLPDVPFAWPEDASRQISSGLDFFQRVMGIRPAGMWPPEG
                     SVSEAALELYMRHGVRWLATDEDILFESLRWGARRDGHGFVINPEVLYKPYRYERNGR
                     DVTIIFRDQSLSDLISFHYSRSDAREAAEDCLGRLRTIRGSIGNRIARPLVTIAMDGE
                     NAWEGYKNDGRDFLSYLYEGILREDGMTPVTISEYLETARDYGSLSRCHAGSWIGHNF
                     SIWLGHSEDNSAWSRLAETRRFLGEEDPDHANSMAWESIYVAEGSDWFWWYGDEHSSD
                     SDEIFDLLFRENLANVYRYLGKEVPEILSIPIIVEDRVARPAREPVNFLRPVIDGRVT
                     NYFEWIGSGFIEGKGHGTAMHEAVFALKDCCFGFNETSLFLRVDLDKRFMNEMDGAVF
                     EVSIIGKDDHKIEYHVRDGSIKAAVPVQAAFDEILELCIPFSDLGLGPKDEAEVWFSL
                     KINEMIVDRIPQRGYLVITVPSETFEA"
     assembly_gap    3805..3814
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3911..5203)
                     /locus_tag="PKJ17_01945"
     CDS             complement(3911..5203)
                     /locus_tag="PKJ17_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD74758.1"
                     /translation="MSPDPSFYYESAEHRQAIDHLTFFLAQQEGFALIYGDVGSGKTT
                     ISRIFLNSLDHGTYNTALILNPVADEAEFMKEVLGEYSVSDIPETKKELYDRLRGYLL
                     EEFQKGRTNVLIIDEAQLLSYDLLEFIRLLSNIETDKQKILHTVFFAQPEFLDKLKDP
                     AMRHLSQRITVTYRIEPLTYAEAKAYINYRLFKAGAKGPLEFQDRAMKLIHTASRGYP
                     RLINYICDRCLLVLYAKSSFSVDGHVVSKVIIEESIPLSTTKHTDDSKRLALRRPLLI
                     GGIIAVIVIGVAGYYFMITGAFRLFAPTRPQTQQASVARTSPAATAAHTTPPAQAPSA
                     PRPAVAAQSSTPARPAAPAKPAQRDIILKRVYVNTEAANVRLEPDINSARIGVIIKDE
                     LLKVIAEKTGADRMKWYKIKLYEGREGWISETVVTERK"
BASE COUNT         1174 a         1477 c         1650 g         1241 t
ORIGIN      
        1 atgaggacgt agtaaaagat gaacagcaaa agactgtaga gggttcccga gaaccttccc
       61 tccacctttc gtctcacccc gagggggatg gtcagtatta taaaggccag tgacgacagg
      121 ggcaaggata tcttcttgta gatctcgaga agcaggtcgt atcgttgatc ctcgttcgcc
      181 gccgtcgtca gcgccttctt cagttcgttc cggtccattt cgtacgcgag ctttctgctg
      241 acgcctcctg tctgcaccat ctgggagagg ttcatggtga aggtgtagtc ctgaaagctg
      301 acgctccgat aggtgtccgt ggccttctcc catcgatgga gggtgccgtc ctgcaacacg
      361 aaatagagct ggaaggtctc cgagtctatg tggacatatc ctttgctcgc ggagatcgtc
      421 tgcttcacgt tcttgtcgcg gtcgtcggac acgacgatgc ccgtcagggt tctcctggta
      481 ttgtcgacct tgttgatgta gatgaccacc cccggcacgc tatcgttgaa gacgccttcc
      541 ttgtcgtcga cggagatgcc tttctttatc acgttgatca gggtttccct gaacaagccg
      601 ctgcagcggg gcaggaggac gttggtgttg aaaagcccgc aggaggtcac tatgagaccc
      661 acgagggtta tcggcacgaa gagccacatg aggttgaccc cgctggcttt cagcgccagt
      721 atctcgttct ccgtggagag tcgcccgagg acaacgatga ctgaaaggag gaaggccatg
      781 gggagggtga aggtgagata agggggcacc gagaaggcca cgagagcgaa gatatccgta
      841 aaccccacac ccttgttgat cacgaggtcg gcgatcttcc cgagtctcga caggacaaga
      901 atgaaagtga ggatgccgag ggagagaagc aggatgccgg tgagttcttt caggagatat
      961 atgttgaact tgaggggaat tttcttcaat aatttcatca cttactctac caccccacgc
     1021 ggtgtcctgt caatgcagat gggcatcggc cttgggtgcg gatgcggaag gtgccagaag
     1081 aagggaaagc ctcccgcctg ccctggtaac gggcaaattt gtttttgaca ggcccccctt
     1141 tttcttatac agttattcat ccaatgaaga tcgccgtcat ttcagacact catctcgctc
     1201 atccgacaga gggtttcaag cgcgctatga aggccctgtt cagcgatgtc gacatgatgc
     1261 tccacgccgg cgacatgacg tcgtcgagcg tcttcgacta cctttcgaac tgggacctcc
     1321 gggcggtcag gggcaacatg gacgatttcg acctgggggc ccgtctcccg gagagccgtg
     1381 tagaggaggc cgcgggaaga cggataggga tcatccatgg gtggggttcg ccgcaagggc
     1441 ttgaggacaa ggtcctccgg gtgttccggg atgtcgatat cattgtcttc ggccattcgc
     1501 acatcccctt caatatgaaa aaaggggggg ccatcctctt caatcccgga agctgcaggg
     1561 gcggatactc ggtatccggg acagtcggga tcattgagat cggcgccgag gtcacgttgc
     1621 gccatcttaa ggttgacgaa ctttaaccat tcggaattgt gagcgataat acgaatatgg
     1681 acgggatctg catctcattt ctctggcaca tgcaccagcc gtattacaag aacaccttca
     1741 ccggggaata cctccttccc tggacccttt tgcacggcac caaggattac tacgacatgg
     1801 gcgccatgct caagggtttt cccggtatga aacagaactt caatttcgtt ccctccctcc
     1861 tcgaacagct cgtggactat gaagacctcg acgtgaaaga tacgtatctc gatgtgttca
     1921 ggaaagaccc ttcgcatctc accgagaaaa acaaggtctt cctcctcggc aacttcttca
     1981 atgccaactg ggagaacatg atccggccct tcccgcgcta ctacgaactc ctcgccaaac
     2041 gcggtctcta ctgtcccgcg gacggcctgt cgaaggttac cgggtatttc agcgaggatg
     2101 agttccggga cctgcagata cttttctttc tcgcctggac cgacccgagc ttcttcgcgg
     2161 actttgaagg tctccggtat ctcagggaaa aggggaaaaa gttcagtgaa gaggacaaga
     2221 aggtcctgat ggacgttcag aagaacatcc tcaagggtac gatccccctc tacagggagc
     2281 ttgccgctga ggggtctgtg gaactgtcga cctcggcctt ctatcacccc atcattcccc
     2341 ttctcatcga cagcgcgtcg gcgagggaag caatgccgga cgtgaacctg ccggacgtgc
     2401 cctttgcctg gcccgaggac gcctcgcgcc agatatcgtc gggtctcgac ttcttccaaa
     2461 gggttatggg tatccggccg gcgggcatgt ggcctcccga gggctccgtc agcgaggccg
     2521 ccctggagct ttacatgcgg cacggtgtcc ggtggcttgc cacggacgag gacatccttt
     2581 tcgaaagtct gagatggggg gcgcgcaggg acgggcacgg cttcgtcatc aatcccgagg
     2641 tcctctacaa gccttatcgt tacgagagga acggccgcga cgtcaccatt atcttccggg
     2701 accagtccct ctccgacctc atatcctttc actattcgcg gtcggacgca agggaagcag
     2761 cggaggattg cctcggcagg ctcaggacga tccggggctc catagggaac aggatcgcga
     2821 ggccccttgt aacgatcgcc atggatggcg agaacgcctg ggaaggttac aagaatgacg
     2881 gaagggattt cctgagctat ctctacgagg gcatccttcg cgaggacggc atgacgcccg
     2941 tcacaatatc cgaatacctt gaaaccgccc gggactatgg aagcctttcc cggtgccacg
     3001 ccggttcctg gatcggacac aatttctcca tatggctcgg ccatagcgaa gacaattcgg
     3061 catggtcccg gcttgcggag acccggcggt tcctcgggga agaagaccct gaccacgcga
     3121 acagcatggc ctgggaatcc atctatgtag cggaggggag cgactggttc tggtggtacg
     3181 gagacgaaca ttcctcggac agcgacgaga tattcgatct cctgttccgt gagaacctgg
     3241 cgaatgtgta ccgctatctc ggcaaggagg tgccggagat actgtcgatc ccgatcatcg
     3301 tggaagaccg tgtggcgcgg cccgctcggg agcccgtcaa ttttctccgt cccgttatcg
     3361 atggcagggt gacaaactat ttcgaatgga taggatccgg cttcatcgaa ggcaaggggc
     3421 acggaacggc catgcatgaa gcggtcttcg ccttgaagga ctgttgtttc gggttcaacg
     3481 aaaccagtct gttcctgcgt gtcgatctgg acaagaggtt catgaacgag atggatggcg
     3541 ccgtcttcga ggtctccatc atcggcaagg acgatcacaa gatagagtat cacgtccgtg
     3601 acgggtccat aaaggcggct gttcccgtgc aggctgcctt tgacgagata ctcgaactgt
     3661 gtatcccttt cagcgacctc ggcctcggcc cgaaggacga ggcggaggtc tggttctcct
     3721 tgaagatcaa cgaaatgata gtcgacagga taccccagag aggctatctc gtgatcacag
     3781 tcccctcgga aacgttcgag gcggnnnnnn nnnnggtacg tttgaagacg gttccagggt
     3841 tcaaggttca agggtttcag ggtagagacg aaaagacccg tgcggcgaag ctaaacgatc
     3901 ggggacgagg ttatttcctt tcggtgacta ccgtttctga gatccagcct tcgcggcctt
     3961 cgtagagttt tatcttgtac cacttcattc tgtcggcgcc ggtcttctcg gcgatgacct
     4021 tgaggagttc atcctttatt atgacgccga tgcgggcgga gttgatgtcc ggttccaatc
     4081 tgacatttgc ggcttcggtg ttgacgtaga ccctcttgag gatgatgtct cgttgtgcgg
     4141 gcttagcggg agcggctggc cgggcaggtg ttgatgattg tgcggctacg gcgggccggg
     4201 gcgccgaagg cgcctgtgcg gggggcgtgg tatgggcggc cgtggctgcc ggtgatgtcc
     4261 gtgcgacaga tgcctgttgc gtttgaggtc ttgtcggggc gaaaagcctg aaggccccgg
     4321 tgatcatgaa gtaatagccg gccacgccta tgacgatgac ggcgatgatc cctcctataa
     4381 ggagagggcg cctcaaggca agcctttttg agtcgtcggt gtgtttcgtc gtggaaaggg
     4441 ggatgctctc ctcgatgatg accttggaga ccacgtggcc gtccacggag aagctggact
     4501 tcgcgtagag gacgaggaga cagcggtcgc agatgtagtt gatgagacga ggatagcccc
     4561 gtgaggcggt gtggatgagc ttcatagccc tgtcctggaa ctcgaggggt cccttggccc
     4621 ctgccttgaa aaggcggtag tttatgtagg cctttgcctc ggcgtaggtc aggggttcga
     4681 tgcgatacgt aacggttatg cgctgggaaa gatggcgcat tgcggggtcc ttcagcttgt
     4741 cgaggaactc cggctgggca aagaagacgg tgtgcagtat cttctgtttg tctgtttcta
     4801 tgttggacag gaggcggatg aactcaagga gatcgtagga gaggagctgg gcctcgtcga
     4861 tgatgagaac attcgtcctg cctttctgga attcctcgag gagatagccc ctgagtctgt
     4921 cgtagagctc tttctttgtc tccggtatgt cgctgacgga atactcgccg agcacctcct
     4981 tcatgaactc cgcctcgtca gccacgggat tcaggataag ggccgtgttg taggtgccgt
     5041 ggtccaggct gttgaggaat atccgtgaga tcgtcgtctt tcccgagccc acatcgccat
     5101 agatcagggc gaagccttcc tgctgggcaa ggaagaacgt gagatgatcg atggcctgcc
     5161 tgtgctccgc ggattcatag taaaaggacg gatccgggga catcccgaag ggcttctccg
     5221 aaaaaccgaa atattcaaga tagggtattt ccatctttta gagaatatga cacaaagaag
     5281 aaaaaaaaga aacaggtaat gggtaaggag ccccaccgtc attccgccaa ctcccccacc
     5341 gtcattcctc caactccccc accgtcattc ctccaactcc cccaccgtca ttccggcgaa
     5401 ggccggaatc caggaagacc tctgtcgcgt cggtaagtgg tggtgccaac cgggaaggtc
     5461 tcaacgtttc ctgcaagatg gtctcggatc ccgaccctct cctggatccc ggccttcgag
     5521 actgtgtcgc aattacctcc accccggtct gt
//
LOCUS       DAOPOA010000053         5489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_16263_length_5489_cov_3.633971, whole genome shotgun sequence.
ACCESSION   DAOPOA010000053 DAOPOA010000000
VERSION     DAOPOA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16263_length_5489_cov_3.633971"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..1333
                     /locus_tag="PKJ17_01950"
     CDS             422..1333
                     /locus_tag="PKJ17_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOD74759.1"
                     /translation="MDSPGTAGKKTTRREAPSPSRRPDTQEVLEQRVQQRTAQLSELN
                     ALLRQEIAERRKMETALQDSRERLRHLSIHLQKIRENERTSLSRELHDEFGQTLTGMK
                     LDVSWIKRRVASDDGLVVERLDSVLADLDRTIIAVQQMSARLRPVALDDFGLRDAVEL
                     AARDVTKRTNIACHIISEPYNITLNDTVTTGAFRILQEALTNVVRHSGAREVTILLRK
                     RKDVFTMEISDDGKGIRKKELVNPRSIGLTGMHERAHALGGTLAIMGVRGKGTSITLT
                     VPLRPVRRDNKRGMDKCSESSSQTTTP"
     gene            1297..1929
                     /locus_tag="PKJ17_01955"
     CDS             1297..1929
                     /locus_tag="PKJ17_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD74760.1"
                     /translation="MLRVIIADDHPVVLKGLREILQENFSGMTFDEARKGYELINKIH
                     QNDYNLVLLDISLPDINGLEVLKEIKKRRTRLPVLIVSMYPEEQYAIRSIKAGAQGYI
                     TKRSASEELVAAVNKILSGKRYINPTFAEQMVIDFESDTEAPPHEKLSIREMQVMKMV
                     ASGLTNKEIAEQLHLSINTVRTYRVRILEKISVKKTNELIWYAVKYGLVE"
     gene            2785..3891
                     /locus_tag="PKJ17_01960"
     CDS             2785..3891
                     /locus_tag="PKJ17_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD74761.1"
                     /translation="MGETIRQGFCALPILMLCLVVSCCVTPTGFDEGAWRKKVEAARA
                     ADLYAPHYKDGKYFNPWMPMEEKGFLRFLKWKLSPSGEYTAEERRFLPSFIPGAAERI
                     EAMPEGDLILWVGHATFLIRLGSRFWLTDPILSDRALLPRRKISPAMTPGELKRLGSP
                     GLNVIISHNHYDHLDKQTLESLPDNTRFFVPLGMKEFLMNLGKKDVIEMDWWQSVDAG
                     DGTTVVCLPAQHWSRRIGQAVNSTLWASFLMISPKNTVYYGGDSGYFIGFREIGRRYP
                     GIDYALIPVTAYHPRWFMHYAHMDIDECIDAFDDLGARYFVPTQWGTFHLGDDPPGLP
                     ALDLKRRIAVRDLDPSRFLIMDIGEILPLSGKTE"
     gene            4003..4527
                     /locus_tag="PKJ17_01965"
     CDS             4003..4527
                     /locus_tag="PKJ17_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74762.1"
                     /translation="MKTKMMVLCALSFLAAACLTAGCGREESATFTTPEGKVTVTAKQ
                     SGAEGTVKVETKEGTATVTTGPQAVNEAELGAPAYPGAQVISSGQFGESKGTRAGSAS
                     AVLLSTNDSFDKVVAFYKANLKDIRQTMDQTAGDQKMVMFMAGREGNMRNVQIVAGAS
                     GGPTNIHIMKVTGK"
     gene            complement(4619..5077)
                     /locus_tag="PKJ17_01970"
     CDS             complement(4619..5077)
                     /locus_tag="PKJ17_01970"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOD74763.1"
                     /translation="MKTRFVMFSAAVILTVLVLASSSSALCVRVSKANLRSGPGKNHE
                     VAWQVYMYMPFEKVGESTDKKWYAVRDCDGDTYWIHRSLVTHRYRCAVVKVQKARVRK
                     GPGTRYSTVKDSPALKYYTYRIISSKRNWFRLRDEWGDIGWIRKDLLWIK"
BASE COUNT         1288 a         1550 c         1559 g         1092 t
ORIGIN      
        1 ggttttcata acccggccct gtgcctcaac atcctcggcc agtccatcga tctggcccac
       61 aaggccaggg aggcagccgg aaaggctgcg ggcatcgccc cgtgacagga atttggagca
      121 actcccaccg tctgcccata taaaaaactt ctgtggtatg ccaaataacc ccatggttac
      181 aatatgttag atgcggtcaa taccatgcct tccattgcgc ttgccggttt ccttgctata
      241 attgtaataa ctcgtatggc gtgcaaggga ggggaaggcg tgatgggggt ttgtcttctc
      301 cggctgcaat taggaaacaa cacgggacgg cattcgaatg cctgatgccg tggtattggc
      361 gtaccggtca taccggccgc gtgagcgcac ggtccgccaa ggatgatcgc aagggggagc
      421 agtggatagt cccggaacgg caggaaagaa aacgacacgg cgggaggctc cgtcaccatc
      481 ccgccgaccg gacacccagg aagtcctgga gcagcgcgtg cagcagcgga cggcacagct
      541 gtcggaattg aacgctctcc tcagacagga gatcgcggag cgcaggaaga tggagaccgc
      601 cctgcaggac tccagggaac ggctacgtca cttaagcata catctccaga agataaggga
      661 aaacgagcgt acctcactct caagagagct ccatgacgaa ttcggtcaaa ccctgacagg
      721 catgaagctt gacgtcagct ggataaagag aagggtcgcc tccgacgacg gacttgtcgt
      781 ggaacggctc gattccgtgc tggccgatct tgaccgcacc atcattgccg tccagcagat
      841 gtcagccaga ctccggcccg tcgctctcga cgatttcggc ctccgggacg cagtggaact
      901 tgccgccagg gatgtcacga aaagaacgaa catcgcatgt cacatcatct ccgaacccta
      961 caacatcacc ctcaatgaca ccgtgaccac cggggcgttc cggatcctcc aggaggccct
     1021 tacaaacgtc gtgcgccatt ccggggcgcg tgaggtcacg atactcctca gaaagcgcaa
     1081 ggatgtcttt actatggaga taagcgatga cggcaagggc atcaggaaga aggaactggt
     1141 gaaccccagg tctatcggtt tgacgggcat gcacgaacgg gcgcatgcac tgggtggaac
     1201 gcttgcgatc atgggtgtgc ggggtaaagg cacctcgatc actcttacag ttccgctccg
     1261 tccggtcagg cgcgacaaca agagaggaat ggacaaatgc tccgagtcat catcgcagac
     1321 gaccaccccg tagtgctcaa gggcctcagg gagatcttgc aggagaattt cagcggcatg
     1381 accttcgatg aggcgcggaa aggctacgag ctgatcaata agatccatca gaacgattac
     1441 aacctggtgc ttctcgacat ctctcttccc gacataaacg gtctcgaagt cctcaaggag
     1501 ataaaaaaga gaaggacacg tctgcccgtc ctcatcgtga gcatgtatcc cgaggaacag
     1561 tacgcgatcc gctccataaa ggccggcgcc cagggttaca taacaaagcg cagcgcgtcg
     1621 gaggaactgg tggccgcggt aaacaagata ctgtcgggca agagatacat caaccccacc
     1681 ttcgcggagc agatggtcat cgatttcgag tccgatacgg aggccccgcc tcacgagaaa
     1741 ctttccatcc gggagatgca ggtgatgaag atggtcgcca gcggactgac gaacaaggag
     1801 atagccgagc agcttcatct cagcataaat accgtcagga cctaccgggt gcgtatcctg
     1861 gaaaagatct ccgtcaagaa gaccaatgag ctgatctggt acgcggtgaa atacggcctc
     1921 gtcgagtgat tacgacttga acctcttgac aggctgctga caaagtcatt tccaaaggcg
     1981 gttcgtgtcc ctccgtcatt ccggtctaaa gttggaatga cggagaaaat gattttatcg
     2041 acagtctgtt gaacgtctcg aaccgtttga atacataaga gacgatcgat ccgccgcaag
     2101 atcccgcagg aacaatctcg ggaggtcatc ggataggaaa cgatgacagc tcttttgaac
     2161 gaaactacca caagataaat gtcgattcgg cgacagacag ggggggggat tcctattgta
     2221 gagtctctat tagagggatc ctggacgtgg agtatttccc cgggcttcat acttgacctt
     2281 ttcacggtgc gttgaggaaa tggccgacag acgggacaaa aagaaggtca cattcaaact
     2341 gcatgagcca tcggctcacg aggtgttcat ggcagggagt atccgtgacg ggacagagac
     2401 cacaaggccg ctcaggaaat gtaaggatgg gacgtggagg acaacgctga gactcagccc
     2461 gggcgtccac gaatatcgta taatcgtgag cggcaccggc cgtgctgaaa ccggtcccac
     2521 gacccggaga gcagcaaaac gcggcatctt caattgcgtc gtgtgggtat agaagaaagt
     2581 ccaaggttca agcagataaa ggcggtttcg ggtttcagga acaggatgcc gttcctgtcg
     2641 tgcaaattga agatccgtcc aataggtcct atcccgtcat atatgtccaa ttggtcctat
     2701 tggtcctatc cacatcctat tggtcctctc tgtccctgtt ccctgtctcc cgcctcctgt
     2761 gctacactgt ggagtggagt gaccatgggg gaaacgatca gacaggggtt ttgtgctctt
     2821 ccgatcctga tgttgtgcct cgttgtctcc tgctgcgtga cacccaccgg gttcgatgag
     2881 ggggcgtgga gaaaaaaggt ggaggcggcg cgcgctgccg acctctacgc gccgcattac
     2941 aaggacggga aatacttcaa cccatggatg cctatggagg aaaaaggctt tctgcgtttc
     3001 ctgaaatgga agctctcacc ctcaggggag tacaccgctg aagagaggag gttcctgccc
     3061 tctttcattc ccggagccgc ggaacgcatc gaggcaatgc ccgaaggcga ccttattctc
     3121 tgggtcggcc acgcgacctt tctcattcgc ctcggctccc ggttctggct gaccgacccc
     3181 atactgtccg acagggcgct cctgccccga agaaagatca gccccgcgat gacgcccggg
     3241 gagctcaagc gcctcggctc gccagggctc aacgtgatca tatctcacaa tcactacgat
     3301 catctggaca agcagaccct cgagtcgctg ccggacaaca cccgcttctt cgtccccctg
     3361 ggcatgaagg agttcttgat gaatctcgga aagaaggatg tgatcgaaat ggactggtgg
     3421 caaagcgtcg atgccggtga cggcacgacg gtggtctgcc tgccggccca gcactggtcc
     3481 cgccggatcg gccaggccgt gaacagcaca ctctgggcaa gttttctcat gatctctccg
     3541 aagaacaccg tctattacgg gggggacagc ggatatttca taggtttccg cgagataggc
     3601 aggcgctatc ccgggataga ctacgccctg ataccggtaa ccgcctacca tccgcgctgg
     3661 tttatgcact acgctcacat ggatatcgac gaatgcatcg acgcgtttga cgatctggga
     3721 gcccggtatt tcgtgcccac tcagtggggc accttccacc tcggtgacga tcctcccggc
     3781 cttcccgccc tcgacctcaa gagaaggatc gccgtcaggg atctcgatcc atcgagattt
     3841 ctcatcatgg atatcggcga aatattgccg ctgtcgggaa agacagaatg atcttcggcc
     3901 ccgtatcgcc cctccccctt tgttgtaatc atttgctccc tgtactataa tggcttcaag
     3961 gcacataccc ggacaatacc taaggatgac agggaggaca gtatgaagac gaagatgatg
     4021 gttttgtgtg ctctgtcctt tcttgccgcg gcatgcctga cggcaggttg cggcagggag
     4081 gaatctgcaa cctttacgac cccggaaggc aaggtgacgg tgacggcgaa acagtcaggt
     4141 gcggaaggca cggtgaaggt ggagacgaag gaaggcaccg cgaccgtcac gacaggtcca
     4201 caggcggtca acgaagctga gctcggcgca cccgcctatc ccggcgcaca ggtgatctct
     4261 tcggggcagt tcggcgagtc aaagggcacc cgggccggat cggcctcagc cgtcctgctc
     4321 agcacgaatg attccttcga taaggtcgtc gcgttctaca aggccaactt gaaggacatc
     4381 cggcaaacaa tggatcagac ggcgggagac cagaagatgg tcatgttcat ggcaggcagg
     4441 gaaggcaaca tgagaaacgt ccagatcgtc gccggagcct ccggtggacc gacgaacata
     4501 cacataatga aggtgacggg gaagtagaac gcgaggtcca caggcaacag gcgacgggat
     4561 acggaaagcc aggaacgcgt tcccctgttg cccgttgcct gtgacccatg acctgctctc
     4621 actttatcca caaaaggtct ttccttatcc agccgatatc gccccactcg tcgcggagcc
     4681 ggaaccagtt tcgttttgag gagatgatcc tgtacgtgta gtacttgagg gccgggctgt
     4741 ccttgacggt ggaataccgt gtacccggcc ccttccgtac ccgcgccttc tgtaccttca
     4801 cgacggcgca ccggtacctg tgggttacca ggctcctgtg tatccagtac gtgtcgccgt
     4861 cgcaatcccg gacggcatac cacttcttgt ccgtagattc ccccaccttc tcaaagggca
     4921 tatacatgta gacctgccac gccacctcat ggttctttcc cggccccgat ctcaggttcg
     4981 ctttcgacac cctgacgcac agcgccgacg atgacgacgc gaggacaaga accgttagga
     5041 tgacggctgc ggagaacatg acgaatctcg tcttcatgga cagtccctcc ctgtactctt
     5101 gcgttgtgat cctgttattc tactttttcg ggaaggacgc gtcggtaaaa cagcttacag
     5161 gaaggaacat taccgggcgc caagaggcgt tccacgggat ccgcccaacc ggggctcccc
     5221 gtctcgaaaa aggaagacag gtcccgcagg actcatgtgg cctgccgggc ctgtctcgtc
     5281 tttctctctt gaacccttga actctggaac cctgaaaccg cctctaatac ggatacacgt
     5341 aaactctccg ggggggtgga tagtagtagt acaccctcgg cggaggtgga gggggcggcg
     5401 gcgatacaac gtacacgggg gccggtcggg tcatggcgct tccaatgatg gtccccgcga
     5461 tcaacccgct tgtaaccccc acaaggccc
//
LOCUS       DAOPOA010000054         5487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_16268_length_5487_cov_3.970177, whole genome shotgun sequence.
ACCESSION   DAOPOA010000054 DAOPOA010000000
VERSION     DAOPOA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16268_length_5487_cov_3.970177"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..571
                     /locus_tag="PKJ17_01975"
     CDS             101..571
                     /locus_tag="PKJ17_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74764.1"
                     /translation="MSSRQVFFVILASMFLVTACGTALNAVQLPEKQVPAAVEKAGKV
                     VPIPKIGVTMMKAQPETYSGACPTVITFKGHIAAAGMISASKPVTVKYVFVRSDGVTG
                     PEQTVTFTKQGAQTVSNTWHIGGPGFSTSGWQAIRIISPDPKESKKANFTLNCN"
     gene            complement(679..990)
                     /locus_tag="PKJ17_01980"
     CDS             complement(679..990)
                     /locus_tag="PKJ17_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74765.1"
                     /translation="MRAVRYCLAILVCSLPILIVPCHAAGQASVQQTMKKYNCGKDRG
                     GTIFCQRIAPAGRFSGQEGIYTEKSEGKVCKWKCRTEKGIETCQASGTECTGKVPPHW
                     R"
     gene            1218..1811
                     /locus_tag="PKJ17_01985"
     CDS             1218..1811
                     /locus_tag="PKJ17_01985"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HOD74766.1"
                     /translation="MTTHRIESLSDCIFAFSMTLLVMTFTFPSAGSPDEVEVVRVFLK
                     EIAKFDRYVLTFALLAILWITHHQQFHHIKRTDRRFLWIQIAILMFVVMVPFTAAWMA
                     AHSHLHITNLVFDVNLFILGLLFLLSWFYATHRTRLVDDNIDRKVVARGMARSLLACS
                     VAVLAMALSFYHPGWSNYAFILTPLLLFLKPLRDTAK"
     gene            complement(1861..3033)
                     /locus_tag="PKJ17_01990"
     CDS             complement(1861..3033)
                     /locus_tag="PKJ17_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74767.1"
                     /translation="MNPTDLLAQSAKLVIQVEEQAAAVVTAIAFFMGSMRTIVTLYRK
                     GYENIIPYLKDQTGRFMIICALCTPLPFLGGTDGKAGTFIGTFPRMVISAGFAQAGNE
                     KVFDNLKAVKAKLGKEMGSGELSEKLTNIKEASPLTGVKIKWVDAIKVRLAQLIFLLP
                     LIPPSSCLALFHPALGAFMMMISYVASEYMAVITCGIGVNVDTSSMVFVLETVRELVL
                     SHMGAITGFLFHTLLVFTFTGVIIAAAVRAAVFCVTFPYSIITMAFDSRREVFFQGIV
                     RAFAIALTPVVAVNVLNVLAMAYDLFMSGGLAGGMIEAYLFSNIDSLVDSGVFPVAGE
                     MYGFVFRLFIATVLAPSVLSIPAIVFLIGSYRVASEAIGAGLSYSGGMGRGGGGGE"
     gene            complement(3194..>5487)
                     /locus_tag="PKJ17_01995"
     CDS             complement(3194..>5487)
                     /locus_tag="PKJ17_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOD74768.1"
                     /translation="EAATCGYLNDAVTASLKGAGFDAGQIHSVVGYKDGWQALNRGYI
                     FDVNINHVAPVVVIDGAPYSFDLWAHGGSEGSFKDFGNSAWNGIKTESWGELMRKHQG
                     YNAFSSDEGITKKDLAEANEELVEKTKANLKAMRIEVRDSYGKPVSGARVTLTHETRY
                     SGTTGMDGSVTAKGVKPAKYAVRVTAEGYLTFSHTAELAPMRDDRYSVTLEPILSSVV
                     ATVRSGSIPVVDAKVRMSSRSPETTGEAGDALFEKVPPGTYTLTAEAEGFAEETRKIT
                     VKPKDEKSAATPVPCNFNLKPRITVRVKGAGQAFTGDVVELEAEVEATESIKPALKYA
                     WRLVGDNTVLASTAKFKRTMVHPGTYVVSIVVYVHRPGVGNMLVGEAVHKIVAEARRI
                     TVSLSGPGESKVGDDVMLRAQIKQLSDSDKEATYTFGWSVNGMKFGGNGETQSLKVTQ
                     AGRNTVRAVVWQAVGGKWVRAGDASHAFVAAGPDPAKGSVSVSGPSSVEVGDRIDLNA
                     SVGDTNVPASALYYSWVVNGRRYNYRESIALPASAPGTYNVTAELWMKYQPKPVKLAQ
                     AFHTVTIVKKGEKPDPLGDLMAAILGGSDKPGTSTGASPTKPDTKVPVGRAYTAAKLP
                     ANQFSLSGTWNAVDSKGRFNGKMSLSQSGSSVSGTMQTPGGGIPVSGSISGSTVSVIL
                     TFGSPAIINQYLQDMRVSQAVGSITAKATFTIGSNNNELQGTLYPFHVQWNDDGKTVI
                     VKRKWQGGETHPGNPPRSFTLRR"
BASE COUNT         1068 a         1708 c         1535 g         1176 t
ORIGIN      
        1 gtgcccgtag gcgtttcatt tgacattttc ccgcgacgga aattacaata gggacatcgt
       61 caaggaaact tggaacaaaa aaacagccag ggagggctct atgtcttcga gacaggtatt
      121 cttcgtcatt cttgcatcca tgtttctcgt cacagcgtgc ggaactgctc ttaatgcggt
      181 gcaactgccg gaaaagcagg tgccggcggc agtcgaaaaa gcgggaaagg tcgttcccat
      241 cccgaagata ggggtaacga tgatgaaggc ccagcctgaa acctactcgg gcgcatgtcc
      301 caccgtcatc acattcaaag ggcatatagc cgccgcgggc atgatcagtg cttcaaaacc
      361 ggtcacggtg aagtatgtct ttgtccggag cgacggcgtt accgggccgg agcagacggt
      421 gaccttcacg aaacagggcg cccagacggt ctccaacacc tggcatattg gaggccccgg
      481 tttttccacc tcggggtggc aggcgatcag gatcatctcg ccggatccga aggaatccaa
      541 gaaggccaac tttacgctga actgcaacta gcatcaggca acattctgcg gtacacgtgc
      601 aggggaaaag gaacaggaca aagccggggg ggttcccgcg accggcaggc tcattcctgt
      661 cccgcgacag cggcgtctct atctccagtg cggcggcact ttgccggtac actccgtgcc
      721 gctcgcctgg catgtctcga tgcccttttc cgtcctgcac ttccatttgc agaccttgcc
      781 ttccgacttt tcggtgtaaa tcccctcctg cccggagaag cgtcccgcag gcgcgatcct
      841 ctggcagaag atggtgccgc cgcggtcttt gccacaattg tacttcttca tcgtctgctg
      901 caccgaggcc tggccggccg cgtgacaggg cacgatcagg atgggcagcg aacatacgag
      961 aatggcaagg caataacgaa cggctctcat ggcttcctcc ttctccatca ttgttgcaga
     1021 gggaattata tcaagtcgaa gcgatacgca caactgtccg gtccgtcatg gtcttaatcc
     1081 cctggagtga aatcccgttc tcttgcctgt atgcccgtca tcccttgtca ttcattcatt
     1141 gacaccgtct tggccgaagt gatataagcg atacgtatat gggcacggca ccgccccctt
     1201 caggcgaacg gttcttcatg acgactcatc gcatcgagag tctgtcggac tgcatctttg
     1261 ccttctcaat gacgctcctc gtcatgacct tcacatttcc cagcgcgggg tctccggatg
     1321 aagtggaggt ggtccgtgta ttcctgaaag agattgcgaa gtttgaccgt tacgtcctca
     1381 ccttcgccct gctggcaatt ctgtggatca ctcatcatca acagttccac cacataaaga
     1441 ggacggaccg ccgttttctc tggatacaga tagcgatcct gatgttcgtt gtcatggtgc
     1501 ccttcactgc cgcctggatg gcggcccaca gtcacctcca catcacgaat ctcgtctttg
     1561 acgtgaatct ctttatcctc gggcttctct ttcttttgag ctggttctac gcgacccaca
     1621 ggacgaggct cgtggacgac aacatagaca gaaaggtcgt agcccggggt atggcgcgat
     1681 cgctcctcgc gtgttccgtc gcagtcctgg ccatggccct atccttctac caccccggat
     1741 ggagcaacta cgccttcatt ttaaccccat tgctcctctt tctgaaaccc cttcgggaca
     1801 cagcgaaata gacgaggtcc atagtcgccc gcaatcgaag agatgcgaat ctccgcgtcc
     1861 tcactcaccc cctccgccgc ccctgcccat accgccggaa tagctcaatc ccgcacctat
     1921 ggcctcggag gcgacgcggt agctcccgat gaggaacacg atggccggga tgctcaacac
     1981 cgaaggcgcc aggacggtcg cgatgaaaag ccggaacaca aacccgtaca tctctcccgc
     2041 gaccggaaaa acaccggaat ccacgagcga atcgatgttg ctgaacagat aggcctcgat
     2101 catcccgccc gcgaggcccc cgctcatgaa aagatcgtag gccatggcga ggacgttgag
     2161 gacgttgacc gccacgacgg gggtcaacgc gatggcgaag gccctgacga tgccctgaaa
     2221 gaacacttca cgccgggagt cgaaggccat ggtgatgatg gaatacggaa aggtgacgca
     2281 gaatacggca gcccttacgg cggcggcaat gatgaccccc gtgaaggtga acacgaggag
     2341 agtgtgaaag agaaaccccg tgatggcccc catgtggctc agcaccaact cacggacggt
     2401 ctcaagaacg aacaccatgc ttgaggtatc cacgttcacc ccgatgccgc aggtgatgac
     2461 tgccatgtat tccgatgcca catagcttat catcatcatg aacgcgccca gggcaggatg
     2521 gaaaagggca agacaggatg agggagggat gaggggcaga agaaagatga gctgtgccag
     2581 ccgcaccttt atggcatcca cccacttgat cttcacacca gtaaggggag aggcctcctt
     2641 gatatttgtc agcttttccg aaagctcccc ggaacccatc tcctttccca acttcgcctt
     2701 cacggccttg aggttgtcaa acaccttctc gttgcccgcc tgcgcgaatc cggcagatat
     2761 gaccatccga gggaatgttc cgatgaatgt accggccttg ccgtcggttc cgcccaggaa
     2821 tggaaggggt gtgcagagcg cgcagatgat catgaatctg ccggtctggt ccttgagata
     2881 ggggataatg ttctcgtagc ctttcctgta aagggtgacg atcgtccgca tgctccccat
     2941 gaagaaggcg atggcggtga caacggcggc cgcctgttcc tcgacctgta tcacaagctt
     3001 cgctgactgc gcgagtaggt ccgtcgggtt cacgggacaa gccccgatgt cctcttattg
     3061 ttcatacagg atatatagca tcgagaacga cagattcccg gccggcgcca gaccgggagc
     3121 cgtggaagcc ggcggaacga accaccggtc cccggcatgg gacttccagg aggtgcggct
     3181 ctctcccggt acgctatcgt ctcagagtga aggagcgcgg gggattcccc gggtgcgttt
     3241 cgcctccctg ccactttcgc ttcacgatca ccgtcttgcc gtcgtcgttc cattgaacat
     3301 ggaaagggta cagggtaccc tgaagctcat tgttgttcga gccgatggtg aaggtggcct
     3361 tggccgtgat ggagcccacc gcctgcgata ctctcatgtc ctggaggtac tggtttatga
     3421 tcgccgggct gccgaatgtg agaatgacgg aaacggtgct gcccgatatc gagccggaaa
     3481 ccgggatccc gcccccgggt gtctgcatgg tgccgctgac gctggagccg gactggctca
     3541 ggctcatctt tccattgaac ctgcccttgg aatcgacggc gttccacgtc ccgctgagcg
     3601 aaaactgatt tgcgggaagc ttcgccgccg tgtacgccct gcccacgggg accttcgtgt
     3661 ccggcttcgt cggggaggcg ccggtcgatg tgccgggttt gtcgcttccg ccaaggatcg
     3721 ccgccattag atcaccgagg gggtcgggct tctccccctt cttcactatc gttacggtgt
     3781 ggaaggcctg ggccagcttg accggtttcg gctggtactt catccacagc tccgcggtca
     3841 cgttatacgt gccgggagcg cttgccggca gggcgatgga ttccctgtag ttgtatcttc
     3901 tgccgttgac cacccagctg tagtagagcg ccgatgccgg cacgttcgtg tcaccgacgc
     3961 tggcattgag atcgatcctg tctcccactt caacggacga cggcccactg acggagacag
     4021 acccttttgc ggggtccgga ccggctgcga cgaatgcatg ggacgcgtcc cccgccctca
     4081 cccacttgcc gccgaccgcc tgccagacga cggcgcggac ggtgttccgg ccggcctgtg
     4141 tcaccttcag agactgcgtt tcaccgttgc ctccgaactt cataccgttc accgaccagc
     4201 cgaaggtgta cgtcgcctcc ttgtcgctgt cgctgagttg ttttatctgc gccctcaaca
     4261 tcacatcgtc gcccaccttg ctctccccgg ggccgctcag gctcacggtg atcctccgcg
     4321 cttcggcgac gatcttgtgg acggcctctc cgacaagcat attccccacg ccagggcggt
     4381 gcacgtagac gacgatgctc accacgtagg tacccggatg gaccatggtc cgtttgaatt
     4441 tggcggtgga ggcaagcacc gtgttatcgc cgacaaggcg ccacgcgtac ttgagggcgg
     4501 gcttgatgga ctccgtggcc tccacctcgg cctcgagctc gacgacatcg ccggtgaagg
     4561 cctgtcctgc ccccttgacc ctgaccgtga tccgcggctt gagattgaaa ttgcacggga
     4621 ccggtgtcgc ggcgctcttc tcgtcctttg gtttcaccgt gatcttccgt gtctcctccg
     4681 cgaacccctc ggcctcggcg gtgagggtgt atgtccccgg ggggaccttc tcgaagaggg
     4741 catcgcccgc ttcgcccgta gtctcggggg accgggacga catgcggacc ttcgcgtcca
     4801 caacaggtat ggaacccgac ctcaccgtgg cgacgacgct gctcaggatc ggctccagtg
     4861 tcacggagta cctgtcgtcc ctcatcggcg cgagttccgc ggtgtgcgaa aaggtcaggt
     4921 acccctccgc cgtcacccgg accgcgtatt ttgccggctt gacccccttt gccgtgacgg
     4981 aaccatccat cccggtcgtc ccgctgtacc tcgtctcatg tgtcaacgtc acccgcgcgc
     5041 cgctcacggg tttgccgtag ctgtccctga cctcgatcct catcgccttc agattggcct
     5101 tcgtcttttc tacaagctcc tcgtttgcct ccgcgaggtc tttctttgtg atcccctcat
     5161 cggaagaaaa ggcgttatag ccctgatgct tgcgcatcag ctctccccag ctttccgtct
     5221 ttatgccgtt ccacgcgctg ttgccgaaat ccttgaaact tccctcggaa cctccatgcg
     5281 cccagaggtc gaaggagtat ggtgcgccgt cgataacgac aacaggcgcg acatggttga
     5341 tgttgacgtc gaagatgtaa ccccggttca acgcctgcca gccgtccttg tatcccacaa
     5401 cggaatgtat ctggccggca tcgaacccag cccccttcag gcttgccgtg acggcgtcgt
     5461 tgagatagcc gcacgtggcc gcttcgc
//
LOCUS       DAOPOA010000055         5441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_16446_length_5441_cov_3.090048, whole genome shotgun sequence.
ACCESSION   DAOPOA010000055 DAOPOA010000000
VERSION     DAOPOA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16446_length_5441_cov_3.090048"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PKJ17_02000"
     CDS             complement(<1..825)
                     /locus_tag="PKJ17_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74769.1"
                     /translation="MVKVAITIDTEEDLWGEYTPAPCPVDNIDSIPLVQDIFDRYGAI
                     PTYLVDYPVVIDDRASRLLRDIREKGGCELGTHCHPWNTPPYGDNASVKATMLCNLEE
                     DAVRRKMENLHETFVARFGMAPVSFRAGRWGFGPAVARSIQDLGYRVDSSVTPLVDWS
                     DYGGPDFFGAPRHPYRLNPASILEPAEDGSLLEAPPTTGFFQKNAELCGKVFRYVMKG
                     NLTRRLKGIGLLEHSRLINLRWLSPELSSGEDMLLLAERLVRHGTSFLNMCFHSTSL"
     gene            complement(873..1799)
                     /locus_tag="PKJ17_02005"
     CDS             complement(873..1799)
                     /locus_tag="PKJ17_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HOD74770.1"
                     /translation="MDSRQKEAKPVMVTVDLEDWFQVENLRPLFPPETWDSCELRVER
                     NVHGLLELFDACRVEATFFVLGWVAERCRGLVREVARAGHEIASHGYSHRLCRELTGA
                     SLRRDIEMSRSLLGDITGEAPAGYRAPNFSITRELTDILAELGFAYDSSYNSFGLNGR
                     YGRAEGLRWGTAGHLVADSGIVELPVSNLTVMGRTVPWAGGGYFRLWPTALFEHGVAR
                     ILQKEGQYVFYCHPWEVDPGQPRQTSGIGWVSAFRHYLNLDRTLHRLEHFLVAFRENR
                     FISCSRYLGVSEYEGAREEHGPGRLREAVPAL"
     gene            complement(1805..3334)
                     /locus_tag="PKJ17_02010"
     CDS             complement(1805..3334)
                     /locus_tag="PKJ17_02010"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOD74771.1"
                     /translation="MEKYLNHETGVPYRPPVQGYLPAGRVEISRGLDPLEAAAAEGQG
                     GKKPQADVEVPLGFPRYRWLLLLGDVVSISLAIIAAIGLRVGFGWKALLHYSIGPVST
                     LMLYPLALYIFDLYNVERVFRSWETTFRSAIAVALGTFLAICVFYVVPLGPYGRGVMI
                     IEASLAWYFLNLWRWSYGIVFQKTGPRTPTLILGAGFCGRTICRLLRSPLSPYEVKGF
                     LDDNLVEIDAEKYPAIMGTSDQLLEAAAGAGAHTVILAIPKNRSVRLIRTILDARLKG
                     IDVRDMADVYEALTGRIPVRGIADQWLLFAQGFYLLRADYAQKLKRLLDFFASGLILL
                     LMSPVIALAVIAIKLDSPGPVFYTQQRVGKDRQVFTIYKFRSMEYGCEKAGARWAAVK
                     DPRVTRVGKVLRLTHIDEIPQIWNIFRGDMSLVGPRPERPEFVDLLEDELPYYYVRHT
                     VKPGLTGWAQINYRYGASVEDANIKLEYDLYYVKNMSIFLDLKILLRTIGVVLLKDGS
                     R"
     gene            complement(3357..4097)
                     /locus_tag="PKJ17_02015"
     CDS             complement(3357..4097)
                     /locus_tag="PKJ17_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74772.1"
                     /translation="MSRIYDALENYREAGAAPLESSPSPPAAVPRAERTNGNGAGMEL
                     EMSTLYQTITAALPGNGHRSVLFVGSRSSEGTSTIAREFSKTVASRMEKGVVLVDCDG
                     SYNGLVYPEMDPDLKLDASLSAGQVEKALCPVDGTSLCILPLFRLSDPGRPQVVDFNN
                     GGGFWDFLKERFELIVVDFPPAMMYTSGPSIIPLVDGVVIVVEAEKTRWQVALSVKEK
                     VIKNGGTVLGVVFNKRQHYIPQFIYNYL"
     gene            complement(4198..>5441)
                     /locus_tag="PKJ17_02020"
     CDS             complement(4198..>5441)
                     /locus_tag="PKJ17_02020"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD74773.1"
                     /translation="SHKEALAAIIYGIEEKKGFVAIVGAVGVGKTTILRSYLEKADKK
                     RLKIIYVFNPRLTFEGLLRTIYQELELPVETSDVVEMTNRLYEVLIEEYRRGNTIVLV
                     VDEAQNMPVDTLENLRMLSNLETSKDKLIQIVLVGQPEFEEELQQHRLRQLRQRLAIR
                     STILPLTEHESMEYIRYRLEKAGVSPSTVFTSTSLKAIVKKAKGIPRTMNILCDNALI
                     TGFGYRKKPVTRGVVREIIRDFDGLKWPSAGRWWFPALSALTVVLIAMAWFLPNHETV
                     FGKIRNLTHMEQRKEDTRVARVVPPPPAATGEALAPPAASPREKESPAGENQPPAPRP
                     GDSAVPPSVVEKATEAVRKSIVHGDTLIKLSREVYGKSDERILRLIQKSNPQVVNPDV
                     IHAGSMLTFPRLSEEGEGTGR"
BASE COUNT         1045 a         1698 c         1618 g         1080 t
ORIGIN      
        1 gagcgatgtc gaatgaaaac acatgttgag gaacgacgtt ccgtggcgca ccaatcgctc
       61 cgcaagcagg agcatgtcct ctcccgagct cagttcggga gagagccacc gaaggttgat
      121 gaggcggctg tgctccagca ggcctatgcc tttaaggcgc ctggtgaggt tccctttcat
      181 gacgtacctg aagacctttc cgcaaagctc ggcgttcttc tgaaaaaagc ccgttgtcgg
      241 cggcgcctcc agtagactgc catcctccgc aggttcaagg atgctcgcgg ggttcaggcg
      301 gtacgggtgc cgcggagcgc cgaagaaatc cggccctcca tagtcggacc aatcgacaag
      361 gggcgtgacg gagctgtcca ccctgtaacc caggtcctga atgcttcgcg cgacggcggg
      421 accgaagccc cacctgccgg cgcggaaact caccggcgcc atcccgaatc gggccacaaa
      481 agtttcgtga aggttctcca tcttcctcct gacggcgtct tcctcgagat tacacagcat
      541 ggtcgccttc acgctcgcat tgtccccgta gggcggcgtg ttccagggat ggcagtgggt
      601 tcccagttca cacccgcctt tctcccgaat gtctcgaagc agacggctcg cacggtcgtc
      661 gatcacgaca gggtaatcca cgaggtatgt cggaatggca ccgtaccggt cgaagatgtc
      721 ctgcaccagc ggaatggaat ctatgttgtc cacgggacag ggggccggcg tgtattcgcc
      781 ccagaggtct tcctcggtat ctatggtaat ggcaacctta accatgtcgc ttcaaaggat
      841 atccaccgcg gtacccttca ttccgcgcgg tgtcaaaggg ccggaactgc ttcccggagg
      901 cgccccggtc catgttcttc ccgggcccct tcgtattcac tgacccccag atagcgagag
      961 catgaaatga agcggttctc ccggaaggca acgagaaagt gttccagtct gtggagggtc
     1021 ctgtcgagat tgaggtagtg cctgaaggcg ctgacccacc ctatgccgga ggtttgacgg
     1081 ggctgtcccg gatccacctc ccaggggtga cagtagaaga cgtattgtcc ttccttttgg
     1141 aggatgcggg cgacgccatg ctcaaagagg gccgtcggcc agagtctgaa atagccgccg
     1201 cctgcccacg ggacggtccg ccccatgacc gtgaggttgc tcaccggcag ttccacgatg
     1261 ccgctgtccg ccacgagatg ccccgcggtg ccccaccgga ggccttcggc ccgtccgtac
     1321 cggccgttga gaccgaaact gttgtaactt gaatcgtagg caaaaccaag ctccgccagg
     1381 atgtccgtaa gttcccgcgt gatggagaaa ttcggcgccc ggtaacccgc cggggcctct
     1441 ccggtgatgt ccccaagcag cgacctgctc atctcgatgt ccctgcgaag ggaagcgccc
     1501 gtgagctcgc ggcacagccg gtgggagtag ccgtgtgaag ctatctcgtg gccggcacgc
     1561 gccacctccc ggacaagccc ccggcagcgc tcggccaccc agccgaggac aaagaacgtc
     1621 gcctccacac ggcacgcgtc gaagagctcg agaagcccgt gcacgttcct ttcgacgcgc
     1681 agttcgcagg aatcccacgt ttccgggggg aacaggggcc gaaggttctc gacctggaac
     1741 cagtcctcga gatcgacggt gaccattacg ggctttgcct ctttctgtcg tgagtccata
     1801 atacctatcg cgacccgtcc ttcaggagga ccacgccgat ggtgcgcagg aggatcttga
     1861 ggtcgagaaa gatggacatg ttcttgacat agtagaggtc gtactcgagc ttgatgttcg
     1921 cgtcctccac cgacgccccg taccggtagt ttatctgcgc ccaccccgtc aggcccggct
     1981 tcacggtgtg acgcacatag tagtagggca gttcgtcttc caggaggtcg acgaactccg
     2041 gcctctcggg gcgcggcccc acgaggctca tgtcccccct gaagatattc cagatctggg
     2101 ggatctcatc gatgtgggta aggcgcagga ccttgcccac ccttgtgacc cgggggtcct
     2161 tcaccgcggc ccaccgcgcg ccggccttct cgcatccgta ttccatggac ctgaacttgt
     2221 agatcgtgaa aacctgccgg tcctttccca ccctctgctg ggtgtagaag accgggccgg
     2281 gggaatcgag cttgatggcg atgacggcga gagctatcac cgggctcatg agcagcagga
     2341 tcagcccgga ggcaaaaaaa tcgaggaggc gcttgagctt ctgcgcgtag tcggcgcgaa
     2401 gaaggtaaaa accttgcgcg aagaggagcc attggtcggc gatgccgcgc accgggatcc
     2461 gacccgtcag cgcctcgtag acgtcggcca tgtcgcggac gtcgatcccc ttgaggcggg
     2521 cgtcgagtat ggtgcgtatc aatcgcacgg accggttctt cggtatcgcc agaatgaccg
     2581 tgtgcgcccc cgcacctgcc gcggcctcga gaagctggtc gctggtgccc atgatggcag
     2641 ggtatttttc cgcgtcgatc tccacaagat tgtcgtcgag gaaccctttc acttcgtacg
     2701 gcgacagggg agatctgagc agcctgcaga tcgtcctgcc gcagaacccg gccccgagga
     2761 tcagcgtcgg agtcctcgga ccggtcttct ggaaaacgat cccgtagctc catcgccaga
     2821 gattgaggaa gtaccaggcg agagaggcct cgatgatcat gacgcccctg ccatatggac
     2881 ccagcggaac gacgtagaag acgcagatgg caagaaaggt cccgagggcc accgctatgg
     2941 cgctgcgaaa ggtagtctcc caggaacgga atacacgctc cacattgtag aggtcgaaga
     3001 tgtagagggc aagcgggtac agcataaggg tggaaacggg tccgatggaa tagtggagaa
     3061 gggccttcca cccgaaaccc accctgagtc cgatggccgc gatgatggcc agagagatgg
     3121 aaacgacgtc gccgagaagc aagagccagc ggtacctggg aaaccccagc ggcacctcca
     3181 cgtcggcctg tggcttcttc ccgccctgtc cttccgccgc ggcggcttcc agggggtcca
     3241 ggccgcggct tatctcgacc cggcccgcag gaaggtatcc ctggaccggc gggcggtagg
     3301 gaaccccggt ctcgtggttc agatattttt ccatgtcctt tccccgtccc tccccgttac
     3361 aggtagttgt aaatgaactg ggggatgtaa tgctgccgct tgttgaaaac gacgccgaga
     3421 acggtacccc cgttcttgat gaccttctcc ttcacgctca gggccacctg ccaccgcgtt
     3481 ttctccgctt cgaccacgat gacgaccccg tcgacgaggg gtatgatcga gggcccgctc
     3541 gtatacatca tcgccggcgg gaaatccacg acgatgagtt cgaacctttc cttgagaaag
     3601 tcccagaacc cgccgccgtt gttgaaatcg accacctgag gccggcccgg gtccgacagg
     3661 cgaaacaggg gcaatataca cagactggtt ccgtccaccg ggcagagcgc cttctcgacc
     3721 tgccctgccg agagactcgc gtccagtttg agatcggggt ccatctcagg gtaaacaagc
     3781 ccgttgtagc ttccatcgca atccacgagg acaacccctt tctccatccg ggacgccacg
     3841 gtcttggaga actcccgggc gatcgtggac gtaccctcgc ttgaacggga ccccacgaag
     3901 aggacggagc gatgcccgtt gcccggaagg gccgcggtga tcgtctgata cagggtgctc
     3961 atctcgagtt ccatgcctgc cccgttgcca ttcgtccgct cggctcgcgg gacggccgcc
     4021 gggggggaag gcgaggattc aagaggtgcc gcccccgctt cccgataatt ctccaatgcg
     4081 tcatagatcc tgctcatgtt gcctccttta cggaatggtt cttctcacca tccctggtcc
     4141 cttccattgc tcgccgccga aacgttttcc gccaaaaaag acgagaagac ggaggcgtca
     4201 tcggccggtc ccttcgccct cctcggatag gcggggaaag gtcagcatgc tgccggcgtg
     4261 gatgacgtcg gggttcacaa cctgagggtt gctcttctgg atcagcctga ggatcctctc
     4321 gtcggatttt ccatagacct ccctcgacag cttgataaga gtatctccat gcactatcga
     4381 tttcctcacc gcctccgtcg ctttctccac aacggacggg ggcacggcgc tgtcgcccgg
     4441 tcgcggggcg ggcggctggt tttcgccggc gggcgattcc ttctcccgcg gcgaggctgc
     4501 gggcggcgcc aacgcctcac ctgttgcagc gggcggcggc ggaacaaccc tcgccaccct
     4561 cgtgtcctct ttcctctgtt ccatgtgtgt aaggttccgt atcttgccga acaccgtttc
     4621 gtgattcggg aggaaccatg ccatcgcaat gagcaccaca gtcagggccg agagggcggg
     4681 aaaccaccac cggccggcgg agggccattt caacccgtcg aaatcgcgta ttatctccct
     4741 gacaacaccc cgggtgacgg gttttttgcg gtaaccgaac ccggtgatga gagcgttgtc
     4801 gcacaggatg ttcatggtcc gcggaatgcc cttcgccttc ttcacgatgg ccttgagcga
     4861 ggtggatgtg aagaccgtcg acggcgagac gccggccttc tcgagcctgt accggatata
     4921 ctccatgctc tcgtgctccg tgaggggcag aatggtcgac ctgatggcga ggcgctgcct
     4981 gagctgcctc agcctgtgct gctgaagttc ctcttcaaac tccggctgcc ccacgaggac
     5041 gatctgtatg agcttgtcct tggacgtctc caggttggag agcatgcgca ggttctccag
     5101 agtatcgacg ggcatgttct gcgcctcatc gacgacgagg acaatggtgt tcccccgcct
     5161 gtattcctcg atgaggacct cgtacagccg gttcgtcatc tccacgacat cgctcgtttc
     5221 gaccgggagt tccagttcct gatagatggt cctcagaagc ccctcgaagg tgagcctggg
     5281 attgaaaaca tagattatct tgaggcgctt cttgtccgcc ttctcgagat aggacctcag
     5341 tatcgttgtt tttccgacac cgacagctcc cacaatggcc acaaacccct tcttctcctc
     5401 tatgccgtaa atgatggcgg caagcgcctc cttgtgactc g
//
LOCUS       DAOPOA010000056         5438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_16461_length_5438_cov_3.364295, whole genome shotgun sequence.
ACCESSION   DAOPOA010000056 DAOPOA010000000
VERSION     DAOPOA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16461_length_5438_cov_3.364295"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKJ17_02025"
     CDS             complement(<1..296)
                     /locus_tag="PKJ17_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD74774.1"
                     /translation="MNRYFHSVTLETEKCKGCTNCIKRCPVEAIRVRDGKAMIVQERC
                     IDCGECIRECPNHAKVAVTDTLDKLSEYKYRIALPAPSFFGQFKEQVNIEDVLGA"
     gene            complement(293..760)
                     /locus_tag="PKJ17_02030"
     CDS             complement(293..760)
                     /locus_tag="PKJ17_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD74775.1"
                     /translation="MVDSLAYTPQITLEFEIGEKDFFVAGECASRVKKTLQQLGLKQD
                     VIKRIAIIIYEAAMNVAIHATSGRLVVHVEPDAITIRTEDVGAGIEDIDLAMKEGYST
                     ASYEIREMGFGAGMGLSNIKQCSDDLNIESRVGVGTTLNARVFFSDERRHEGA"
     gene            complement(783..1133)
                     /locus_tag="PKJ17_02035"
     CDS             complement(783..1133)
                     /locus_tag="PKJ17_02035"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOD74776.1"
                     /translation="MTLQEVQELLEAQVLCGNDECLARSVRVCFACDLISEMLLYLKP
                     HSLLITSLTNAHVIHAAQVMDAKAVLFVGGRRPDESVVRSGELNKIPLLTTMLLTFDC
                     CGRLYEKGIRGDKQ"
     gene            complement(1353..2912)
                     /locus_tag="PKJ17_02040"
     CDS             complement(1353..2912)
                     /locus_tag="PKJ17_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD74777.1"
                     /translation="MDADEQEFFREMTLKICGTLDLESALHQCFLYVRNIIPADELDL
                     FVYDPAIGTVDVAASANEAGGIKRMDKTHLPPELRQELEEPYRRPRVRVAENIFEDPI
                     LGRVARNVGWEECSIMLGRLIIDGKYIGALGIRAKGAGQFTEDHVRLWSLVNEPAAIA
                     VANSRRLQEVIELKDMLADDNRYLRDELRKNTVEIVGADFGLKEAMESVRKVAPLASP
                     VLIIGETGTGKEVIANAIHNLSPRYSGPFVKVNCGAIPESLIDSELFGHEKGAFTGAL
                     ARKRGRFERAHGGTIFLDEISELPPHAQVRLLRVLQEKEIERVGGTEPVHVDIRIVSA
                     TNRNLEKMVEDGDFREDLYFRIKVFPIEVPPLRVRKNDIPALVQHFVRKKSREMVRHT
                     IPPLAPGAIDALMTYDWPGNVREVENAVERALILSDGKPLKFDGVLGVSGASQERAPA
                     PAGESRGFVTLHDMEAEHISRILRAAGGRIEGPGGAAELLGMNPGTLRHRMRKLGIPF
                     GRKSRARDGGR"
     gene            3090..3404
                     /locus_tag="PKJ17_02045"
     CDS             3090..3404
                     /locus_tag="PKJ17_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74778.1"
                     /translation="MEWIEIIRLRTQPDVEPLVVKWLKDLIHSIGGTPGLNEARIYTH
                     SAVPGDVSLHILWDTMQNIYRESEIGLMAAETLKKYGILDHTVWLMKGNNGSDPHRQV
                     TR"
     gene            4020..4712
                     /locus_tag="PKJ17_02050"
     CDS             4020..4712
                     /locus_tag="PKJ17_02050"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOD74779.1"
                     /translation="MNEMEVSMANVEDRNDLPTKPIPEPTLRRLPLYYQYLKKVRDEH
                     KGDFISCTQIGNDLSILPIQVRKDLGIAEAVGKPKLGYSIDELISIIETFLGWNNTKD
                     AYLVGVGHLGTALLGFERFKEYGLNIIAAFDIDKEKIGKTIHGVKVFHMGKLHNMVKR
                     MGIKIGILTVPASPAQELTDEMVRAGIRAIWNFSPVKVNVPPDIIVQHENLASSLVVL
                     AKKLTLARQNQS"
     gene            4728..5234
                     /locus_tag="PKJ17_02055"
     CDS             4728..5234
                     /locus_tag="PKJ17_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOD74780.1"
                     /translation="METMRKFERVCEIIDNNQRNAARLIPILQAVQAEYRYLPEEILT
                     FVATSLGISPARVFGVATFYSLFTLKPKGKYLIRVCNGTACHVKGSMGIYGAIVKKLG
                     LAEGQDSTSDMLFTVETVNCLGACGLSPAMVVNDEVFGQLTPQRVSDIIDEIVLKEAQ
                     NESDAHGA"
     gene            5209..>5438
                     /locus_tag="PKJ17_02060"
     CDS             5209..>5438
                     /locus_tag="PKJ17_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOD74781.1"
                     /translation="MNLMLTGLNKITNDFHVRSAAARHRILVCAGTGCLVNGSLKVYE
                     AFVNTLKESGLNAIVELKKEEEGEFVSRTGCQG"
BASE COUNT         1204 a         1614 c         1435 g         1185 t
ORIGIN      
        1 gccccgagca cgtcctcgat attcacctgt tccttgaact ggccgaagaa cgacggcgcc
       61 ggcagggcga tccggtactt gtattccgaa agtttatcga gtgtgtccgt taccgccact
      121 ttcgcatggt tcgggcattc gcggatgcat tcgccgcagt cgatgcagcg ttcctgaacg
      181 atcatcgcct tgccgtcacg gacacggata gcctcgacgg gacaccgctt gatgcaattg
      241 gtgcatccct tgcacttttc ggtctccagc gtcacggaat ggaagtatcg gttcatgcgc
      301 cctcgtgccg cctctcatca ctgaagaaga ccctggcgtt cagagtcgtt cccacgccca
      361 ccctggattc gatattgagg tcatcggagc actgtttgat gttggaaagg cccatgccag
      421 ccccaaaccc catctcccgg atctcgtagg aggccgtcga atacccttcc ttcatggcaa
      481 ggtcgatatc ttctatcccg gcccccacat cttctgtccg tatggtgatc gcgtcaggct
      541 cgacatggac gacaagcctt cccgacgtgg cgtggatggc cacgttcatc gccgcctcat
      601 atatgatgat cgcgatccgc ttgataacat cctgtttgag cccaagctgc tgaagggtct
      661 tcttcacccg agacgcacac tctccggcaa caaagaaatc cttttcgcct atctcgaact
      721 cgagcgtgat ctgcggggtg tatgctaagc tgtcaaccat tggttagtct ctcctaaccg
      781 ggctattgct tatctcctct gatacccttc tcgtaaagcc ggccgcagca gtcgaacgtg
      841 agaagcatcg tggtcagcag ggggatcttg ttcaattccc cgctcctgac aacggattca
      901 tcgggcctgc gtcccccgac aaagaggacc gccttcgcgt ccatgacctg tgccgcatgg
      961 atcacgtgcg cgttggtcag cgacgtgatc agaagcgaat ggggcttcag gtaaagaagc
     1021 atctcgctga tgaggtcgca ggcgaaacaa acccgcacgc tacgcgccag acactcgtcg
     1081 ttcccgcaga gcacctgcgc ttccagcagt tcctggacct cttgcaatgt catgcacaac
     1141 tcccgtcgtc cggatgtcaa ccggtggtgt atcgatactg ttgtcgcgcc ctggttccgc
     1201 gaaccagcaa agcggcctct caatcaactt cagaaagcct ggctgccggg tatgttgtct
     1261 gtttagttac agggacgagc acgcttgttc agtatagtgc attttgtttc cgaagtcaaa
     1321 aggacagtga agaaaaaaaa caatattttt cgttaccgtc cgccatcccg tgcacgggat
     1381 tttctaccga aaggtattcc cagttttctc atgcgatgac ggagagttcc ggggttcatg
     1441 ccaagaagtt cggcggcccc cccggggccc tcgatcctgc ccccggccgc tctcaggatg
     1501 cggcttatat gctcggcctc catgtcgtga agtgtgacga agccccgact ctcgcccgcg
     1561 ggggccggag cgcgctcctg ggacgcgcct gaaaccccca ggacaccgtc gaatttcagc
     1621 ggcttgccgt cggaaaggat gagggccctc tccacggcgt tctccacctc ccggacattc
     1681 cccggccagt cgtatgtcat gagcgcatct atggcccccg gcgcaagcgg cggtatcgtg
     1741 tggcgcacca tctcccggct cttcttgcgg acaaaatgct ggaccagagc gggtatgtcg
     1801 ttctttcgga cccgcagagg ggggacctcg atgggaaaga ccttgatgcg gaaatagagg
     1861 tcctcccgaa agtctccgtc ttccaccatc ttctccaggt tcctgttcgt ggcagacacg
     1921 atcctgatgt cgacatggac gggttcggtt ccgcccactc tttctatctc tttctcctgg
     1981 aggacacgaa gaagcctcac ctgcgcgtgg ggcgggagtt cgctgatctc gtcgagaaag
     2041 atcgtccctc cgtgggcacg ctcgaaacgc ccccttttgc gtgcgagcgc tcccgtaaaa
     2101 gccccctttt cgtgaccgaa cagctcgctg tctatgagag actccgggat cgccccgcag
     2161 ttcactttca caaaaggtcc gctataccgg ggagacaggt tgtggatggc gttggcgatg
     2221 acctctttgc ctgttcccgt ctcgccgatg atgagcaccg gactggcaag aggcgccacc
     2281 tttctcacgc tttccatggc ttccttcagt ccgaagtccg cccccactat ttcgacggtg
     2341 ttcttcctga gctcatccct gaggtaccgg ttgtcatcgg caagcatgtc tttgagctcg
     2401 atcacttcct ggagcctgcg gctgttggcg actgcgatcg cggccggctc gttgaccagc
     2461 gaccacaggc ggacatggtc ctcggtgaac tggcccgccc ctttcgcccg tatccccagg
     2521 gcgccgatgt acttgccgtc gatgatgagc cttcccagca tgatggagca ttcctcccat
     2581 ccgacatttc gtgcaaccct ccccagaatg gggtcctcga agatgttctc ggcgacgcgg
     2641 acccggggcc tgcggtatgg ctcttcgagc tcctgtcgca gttcgggcgg caggtgcgtc
     2701 ttgtccatcc tctttatgcc gcccgcctcg tttgccgaag ccgccacgtc cacggtgccg
     2761 atggccggat cgtatacgaa aagatcgagc tcatcggccg gtataatgtt gcgcacgtag
     2821 agaaaacact gatgaagcgc gctttccaga tccagggtac cgcaaatctt cagggtcatc
     2881 tcccggaaga actcttgctc gtcggcgtcc atggcttttt ctcctttgtt ctactatcgt
     2941 atgcgatagt atagaccaga actgacaaat ataccagcaa ttttccgtca catttcacag
     3001 taatctggcc atgataatgt aacacgctga atatactaga aaataagttc tggcacggca
     3061 aatgcttaag gattgacaag aaagaatgta tggaatggat agagatcata cgattgagaa
     3121 cgcaacccga tgtcgagccg ctggtggtca agtggctgaa agacctgatc cacagcatcg
     3181 gcggaacgcc cggtctcaac gaagcacgca tctacaccca ttcggccgtc cccggggatg
     3241 tgagcctgca tatattgtgg gataccatgc agaacatcta cagggaaagc gagataggat
     3301 tgatggccgc cgaaacactg aagaaatacg gcatcctcga tcataccgtg tggctcatga
     3361 aggggaacaa cggcagcgac ccacaccggc aggtgacgcg atgatatccg cggcatcatt
     3421 gaccactatt tacgacctat cagtggcggg aaatgtgaag taccggaaat ccctcacctt
     3481 tccttcccat aacgtgagtc gggggacatg aagaatgtcc gacctgatac tgaataacct
     3541 catgctcatg ctgaccctca tattcgcctc cacaactatc tggatcacct ccaagccctc
     3601 tctgcgcaca cgatcgagct cctcatacca cctccacttc atacctcgcc taccggcaga
     3661 cgaaaccacc gctgtcggca aggatctcaa atggtgtacc cgaggccatg cccccgactc
     3721 acaaattaaa tcgcaattga ggctctgaag agtcatcttc acgataccat cacccccccc
     3781 cttatgcgtt tttaaatgcc cccggcgcct cccgggggca acccttttaa agaccgttgc
     3841 agccgctgaa tcgcttgaac gcgtgaacgc tccaaaagaa cagcgaactt actgctgtcc
     3901 cggcgaccct atcaagttat ctccgggtcg gtcgatatta ttctgagaaa aatattgact
     3961 cggggagggt tattcgatat atttttatcg atacattttt atagatagga aattatcgaa
     4021 tgaacgagat ggaggtaagc atggcaaatg tggaggacag aaacgatctt ccgaccaaac
     4081 ccatccccga accaacgtta aggaggcttc ctctctatta ccagtacctg aagaaagtgc
     4141 gtgatgagca caagggggat ttcatctcct gcacgcagat cggcaacgac ctgagcatac
     4201 tccccatcca ggtccgcaag gacctgggga tcgccgaggc cgtcgggaaa ccgaagctcg
     4261 gctacagtat cgacgagctg atctccatca tagagacctt cctcggctgg aacaacacca
     4321 aggatgccta cctcgtcggt gtcggccacc tcgggacggc actcctcggg ttcgagaggt
     4381 tcaaggaata cggtctcaac atcatagccg ccttcgatat cgacaaggag aagataggaa
     4441 agaccattca tggggtcaag gtctttcaca tgggcaagct ccacaacatg gtgaaacgca
     4501 tgggcatcaa gatcgggata ctgaccgttc cggcatcccc cgcccaggaa ctgacggatg
     4561 aaatggtacg cgcgggcata cgggcgatct ggaacttttc gccggtgaag gtgaacgtcc
     4621 ctcccgacat catcgtccag cacgagaacc ttgcctcgtc cctcgtggtc ctggcaaaga
     4681 aactgacact ggcaagacaa aaccaatcgt aaggatggga tgaaataatg gagacaatgc
     4741 gaaaattcga acgggtctgc gagatcatcg acaacaacca gcgaaacgcc gcgaggctca
     4801 tacccattct ccaggcggtg caggcggaat atcgctacct gccggaagag atattgacct
     4861 tcgtggcaac gtcccttgga atatctcccg cacgggtgtt cggggtggcg accttctatt
     4921 cgctcttcac cctgaaaccc aagggcaagt acctcatacg ggtctgcaac gggaccgcgt
     4981 gccatgtcaa gggctctatg ggcatctacg gggcgatcgt gaagaagctc ggactcgccg
     5041 agggacagga ttccacgtcg gacatgcttt tcaccgtcga gaccgtcaat tgccttggcg
     5101 cctgcggcct ttcgccggcc atggtcgtca atgacgaggt cttcggccag ctcacgccgc
     5161 agcgggtatc ggacattatc gacgagatcg tactcaagga ggcgcaaaat gaatctgatg
     5221 ctcacggggc ttaacaagat aaccaacgat tttcacgttc gctccgcggc cgcccggcac
     5281 cgtatcctcg tctgcgccgg aacgggatgc ctggtgaacg ggtccctcaa ggtatacgag
     5341 gcgttcgtta acacgctgaa ggaaagcggg ctcaacgcca tcgtcgagct caagaaagag
     5401 gaagagggtg aattcgtatc gcgcaccggc tgtcaggg
//
LOCUS       DAOPOA010000057         5392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_16637_length_5392_cov_2.946412, whole genome shotgun sequence.
ACCESSION   DAOPOA010000057 DAOPOA010000000
VERSION     DAOPOA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16637_length_5392_cov_2.946412"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1141
                     /locus_tag="PKJ17_02065"
                     /pseudo
     CDS             149..1141
                     /locus_tag="PKJ17_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
     gene            1410..1928
                     /locus_tag="PKJ17_02070"
     CDS             1410..1928
                     /locus_tag="PKJ17_02070"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HOD74782.1"
                     /translation="MKDTLNARVKHREGFRPFAPSVLLENASEYFDLDCESPYMLLIA
                     EVKEDKRHLIPAITHVDGTARVQTVTREDNGRYYELISEFGRITNVPVILNTSYNIAG
                     EPIVETPGDALKCFMSTEMDYLIIEDYIIEATGPKELIHGPVIDENKLKAVDIRQNQW
                     LGKLFSKKKKTL"
     gene            complement(1987..2466)
                     /locus_tag="PKJ17_02075"
     CDS             complement(1987..2466)
                     /locus_tag="PKJ17_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOD74783.1"
                     /translation="MEIGRAIKERAEMLEDTSLSMDFSWEELVTLAGYMGTMTYRKGT
                     TIFEEGDREIFMCVVVKGGVAILKKDERMDPKRLTVIGLGKVIGEMSMIDGSPRSATA
                     TAAADTILVVLSKESMDRLFKEKPHVGLKLLKKIAHSMSQRLRQTTGVLVDYLERSG"
     gene            2593..2832
                     /locus_tag="PKJ17_02080"
     CDS             2593..2832
                     /locus_tag="PKJ17_02080"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOD74784.1"
                     /translation="MGGSVYVVNEIVGTSTTSWEDAAKTAIETAAKSVHDLRIGEVVT
                     QDVTLENGKVVSYRVRLNISFKYHPDKVWHEEVKR"
     gene            complement(2974..3654)
                     /locus_tag="PKJ17_02085"
     CDS             complement(2974..3654)
                     /locus_tag="PKJ17_02085"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOD74785.1"
                     /translation="MDRERIERIIENKYSLLLFFLVILLAGSAFTAHNDMMHYTVGSL
                     FAMVVFIIMLKILRASRLLFAAYITLSFLALLLHYLAVFVVHSKSLGTVAIIVYILMI
                     GILIVFMTRRIFSERLVTGDTIKGGISVYIMMGTWWQMVYQLLWMCDPRSFLFPEGTV
                     GQGDFLYYSISTITCLGFGDILPRSYPAKLFSVIEALVGQLYVAVFVARLVGLHVAVH
                     ARKHNDGR"
     gene            complement(3836..4468)
                     /locus_tag="PKJ17_02090"
     CDS             complement(3836..4468)
                     /locus_tag="PKJ17_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD74786.1"
                     /translation="MTHAFKVLVTDDDPIVLELTSSLLRQAGYEVLQATTGQECLETV
                     RTHHPDLIILDVMLPDVTGTDICRKVKNDPGLQGSFVILLSGVHISSNQQAEGLSAGA
                     DGYLVKPVSRKEFIARVQAMERIKRAEDALRETKKEQEATIQELRKALEEIKTLRGLI
                     PICAWCKRIRDDAGYWDELEVYLGKHSEATFSHGLCPRCREEVEKGMKGE"
     gene            4888..>5392
                     /locus_tag="PKJ17_02095"
     CDS             4888..>5392
                     /locus_tag="PKJ17_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOD74787.1"
                     /translation="MSVYLASVIFVVLAEMGDKTQLLAMAFASRYRWQTVLWAVFVAT
                     LVNHFFAVVAGSYLTRFVPIQYIQIAASASFILFGLWTIRGDTLEGEDKRFNFSPFWT
                     VAVAFFFAEMGDKTQLATVALATKYSNILVVWFGTTTAMIIADAIGIIIGIVMGKKIP
                     ERFVKWFA"
BASE COUNT         1187 a         1605 c         1502 g         1098 t
ORIGIN      
        1 aacagggaaa gggaaaacag gtaggcccgc ctggggtgcg gtacccctgc cccgcctggc
       61 ctctcattcc ccgacctctt tggcgttgac tgcgtgaaac ctctctgtta tagtcaacaa
      121 gcatcacaaa aatcagccag gagaggtcat gaatattctg ggtcttcaca tattcggcca
      181 cgacaccgga gcggcgctca tatcggaagg aagattgatg gccattgccg aagaaagggt
      241 cgacaggcgc aaacattccg gcagtttccc cgtgatgtcc gtcaactatc tcctgaaagc
      301 aacgggcctc aagaacgtta acgacgtcga cctcgtcgtg gcagtgacac gctacggcaa
      361 agacggcaga gacaaaacgg gcgacagcat ccgggaaaga ctgggttatt cgggaaggat
      421 ccatacgatc tcgcaccacg aggcccatgc cgcgagcgct ttcttcgctt cacccttcga
      481 cgaagcggcc gtgatggtcg ttgacggcct cggaagcagc gcgctggaga tcgagacgga
      541 cagacccctg ccattcatgc tgcgtggtct cccgttcaag gacgtcaaga actacgaaga
      601 ggtccagtcc ttttacaggg cccgggggaa cgagataacg ggcatcagga aggactacac
      661 cgtccccggt ttcatgaacg gcatcgggct tgtctacatg ggcacctcgg tgttcctcaa
      721 ttttggcgac ttcggctccg gcaaggtgat ggggctcgcc ccctatggcg gacaaccggg
      781 aaaggtaatg gacgagccct tcgtggagat cgtcgatggt gtcgccctgg caggcagcga
      841 gaaaaatttc gtccgccaca acgatctgta ccgcaaacgc ttcttcccgg acataccccc
      901 gcggcaggag gaaaagctcc ccgacgacgt ctacaccgag atcgcctacg ccgtccagaa
      961 ggcgacggaa gacgccctga tcgaaatggc ccgccacctc taccggataa gcccctcgaa
     1021 gaacctgtgc tacgccggcg gtgtcggcct caacagcgtc gccaacaaga agatactcga
     1081 caacacgcct ttcgaaagga tattcatcca gcccggagcc tgtgattccg ggatcgccct
     1141 gggatgcgcc cttcacggcg cccatatcat ccaccgggag cctcccggca agtatcgcct
     1201 ccgcaatgcc tacctgggcc gggcatacag cgaggaggaa atcctcgccg accttgacca
     1261 ggtgaagaac atcagattca ggaaggagcg ggatgtggcg ctcaaggccg cggagctttt
     1321 ggccgggggc aagatcctcg gctggttcga ggggggcagc gagataggcc cccgcgccct
     1381 gggccacagg agacccgaga aagccggaga tgaaggatac gctcaacgcc cgggtaaagc
     1441 accgggaggg tttccggcct ttcgcgccgt cggtccttct cgagaacgcc tccgagtatt
     1501 tcgacctcga ttgcgagagt ccctatatgc tcctcatcgc cgaggtgaaa gaggacaagc
     1561 ggcacctcat acccgccata actcacgtgg acggtactgc cagggtccag accgtcacac
     1621 gagaggacaa tgggaggtac tacgagctta tcagtgagtt cggcaggatc acgaatgtcc
     1681 ccgtcatcct caacacgtcg tacaacatcg ccggggaacc gatcgtcgaa acgccggggg
     1741 atgccctgaa atgcttcatg tcgacggaaa tggactacct catcatagag gactacatca
     1801 tcgaggcgac agggcccaag gaactcatcc acggccccgt tatcgacgag aacaagctga
     1861 aggcggtcga tatccggcaa aatcagtggt tggggaagct cttctcgaag aagaaaaaga
     1921 cgctgtgacc ggtgtcagag gtccatcccg gtccgtcccc ggtgccgcct cggctgacgg
     1981 gatgactcag ccgcttcttt caagataatc cacgagcacg cccgtcgtct gacgcagtct
     2041 ctggctcatg gaatgggcga tcttcttgag cagtttgagg ccgacgtggg gtttttcctt
     2101 gaaaagacgg tccatgcttt ccttcgagag gacaaccagg atggtatcgg cggcggccgt
     2161 cgccgttgcg gagcgagggc tcccatctat catggacatc tcgccaatga cctttcccag
     2221 acctataacg gtcaggcgct tcgggtccat gcgctcgtcc ttcttcagga tagcgacgcc
     2281 gcccttcaca acaacgcaca tgaagatctc cctgtcgccc tcctcgaaga tcgtcgttcc
     2341 cttcctgtag gtcatggtgc ccatgtaacc cgcgagagtg accagttcct cccacgaaaa
     2401 atccatgctc agcgatgtgt cctcgagcat ctccgcccgt tcctttatgg cacgtccgat
     2461 ctccattctt ctctctcctc aatgcagtat tatcgccatt tccgggccgg actgaaggct
     2521 cgcccttctt gacacgcctt tcatttgtgc tattaacacc ttataacact cgcggacagc
     2581 aaggaggttg tcatgggagg aagcgtgtat gtcgtcaatg agatcgtggg caccagcacg
     2641 acatcatggg aggatgcggc aaagacggcc atagagaccg ccgccaaaag cgttcatgac
     2701 ctccgtatcg gagaggtcgt gacccaggat gtgaccctgg agaacggtaa ggtggtgagc
     2761 taccgcgtca ggctcaacat ctccttcaag tatcacccgg acaaggtctg gcacgaggag
     2821 gtcaagcgct gagcaagaga cattccctgc aacctcagac cacgtacccg ccgcccggcc
     2881 ccgttcctcg aaggtgagat atccggcggc accccccgcg ggcttcaccg cggcagaagc
     2941 cttgcctgat cgggagaaag acggtaatgc cggctagcgt ccgtcgttgt gcttccgtgc
     3001 gtgtaccgcc acgtgcagtc ctacgagacg ggcaacaaag accgccacgt aaagctgacc
     3061 cacaagggcc tcgatcacgg agaagagttt tgccggatag gaccggggta gtatgtcacc
     3121 aaatcccagg catgttatgg tgctgatgct gtaatacagg aagtcaccct gaccgaccgt
     3181 gccctcgggg aaaaggaagg accggggatc gcacatccac agcagctggt agaccatctg
     3241 ccaccacgta cccatcatta tatacacgga gatcccgccc ttgatggtat cgcccgttac
     3301 cagcctctcg gagaatatcc gacgcgtcat gaacacgatg agtatcccga tcatcagtat
     3361 gtagacgata atggcaaccg ttcccaggga cttgctgtgc acgacgaaca cggcgaggta
     3421 gtgcaggagc agcgccagaa aagacagggt tatataggcg gcaaagagga gccgggaggc
     3481 cctcaggatc ttcagcatga ttatgaaaac gaccatcgcg aagagcgaac caacggtgta
     3541 atgcatcatg tcattgtgag cggtaaaagc tgaacccgcc agcagaatga cgagaaagaa
     3601 gaggaggagg gaatacttgt tctcgataat gcgttcgatg cgttctctgt ccaaccgtgc
     3661 ccccttgaga ggatcttccc tgccgacggg ccgatctatt catctccccg ccttccctgt
     3721 caccaacgta gaataccgcc tgtggcgggc tgacatttta ttgtgcagca aatgctgaca
     3781 atttacaaga tggaagagag aacgtgggag tgcctcaaga catgcgggac cttttctatt
     3841 cgcccttcat gcccttttcg acttcttccc tgcatctcgg acaaagcccg tggctgaagg
     3901 tcgcttcact gtgcttgccg agatatacct ccagttcgtc ccagtatccc gcgtcgtccc
     3961 gtatcctttt gcaccacgcg cagatcggta tgagacctct gagcgtcttg atctcttcga
     4021 gggcttttct aagctcctgg atcgttgctt cctgctcctt cttcgtttcc ctgagcgcgt
     4081 cctctgcacg cttgatccgt tccatcgcct gtacccgggc aatgaactcc ttcctggaga
     4141 ccggcttgac gagataaccg tcggcacctg ctgaaagacc ctctgcctgc tggttcgacg
     4201 atatatggac tccggaaaga aggatcacga aggaaccctg caggccggga tcattcttga
     4261 ccttccggca tatgtctgtc cccgtcacat ccggaagcat gacgtcgagg atgatgaggt
     4321 ccggatgatg ggtacggacc gtctcgaggc actcctgtcc cgtcgtggcc tgcaacacct
     4381 catatcccgc ctgccggagg agggaggacg tcagttcgag gacaatggga tcatcgtccg
     4441 tgacaagtac cttgaatgcg tgggtcacgc tgtccacctc ctcacctgtc ctatattcca
     4501 tcacagatac acggggacgt caaccgatgg acccccgaaa aacccattgt cctttcagta
     4561 agttccagcc ggcaccatgt tgccttgaca cgcgcggagc cgaccgtgag tgaatcgccc
     4621 gtacggcaaa tcccgccaac gtgcaggcgg gcaccctgcc cgccggtgca cgcgcttctt
     4681 tccatgggca attcttgatt tttgacgttt ttccggctta taatagtccc gcgttgttga
     4741 aggggagtag ctagacaggc aggatcaaca tgctggggcg accctggtcc tgccgttggc
     4801 aggcataagc caattagcga gaccttcggt atgcgatcgt accgcgtact gaagagtctc
     4861 gttttttttg gcacttcggg ggttcgaatg agcgtatatc tggcatcggt catcttcgta
     4921 gttctcgccg agatggggga caagacccag cttctcgcga tggccttcgc gtcgcggtac
     4981 cgctggcaga cggtgctgtg ggcagtcttc gtcgccaccc tcgtcaacca tttcttcgcc
     5041 gtcgtggccg gaagctatct cacgcggttc gtccccatac agtacattca gatagcggcc
     5101 tccgcctcgt tcatactctt cggtctctgg accatccgcg gggacacgct ggagggggag
     5161 gacaagcggt tcaacttcag ccccttctgg accgtggccg tggccttctt cttcgccgag
     5221 atgggggaca agacccagct cgccaccgtg gccctggcaa caaagtacag caatatcctc
     5281 gtggtctggt ttggtaccac aacggcgatg atcatcgccg acgccatcgg catcatcatc
     5341 gggatagtta tgggcaagaa gatacccgag cgcttcgtca aatggttcgc cg
//
LOCUS       DAOPOA010000058         5310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_16974_length_5310_cov_3.299905, whole genome shotgun sequence.
ACCESSION   DAOPOA010000058 DAOPOA010000000
VERSION     DAOPOA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16974_length_5310_cov_3.299905"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKJ17_02100"
     CDS             complement(<1..204)
                     /locus_tag="PKJ17_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74788.1"
                     /translation="MMRSVLCVVFAVVVWFGSGGYAAGASVELFSPEGSVKDVSQVTV
                     RFSEQMVAFGDPRPADPFAMQCAG"
     gene            complement(418..834)
                     /gene="nikR"
                     /locus_tag="PKJ17_02105"
     CDS             complement(418..834)
                     /gene="nikR"
                     /locus_tag="PKJ17_02105"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOD74789.1"
                     /translation="MSQLVRFGVSLDGDLLKKFDELIREKSYSNRSEAFRDLIRQELI
                     AKEWVVGEEVAGAITLIYDHHHRELLNKVTDLQHDYQHTIISTQHVHLDHNNCLEIVA
                     VRGKPDEVRRLAEMLRAIKGIKHCTLSMSSTGKGLE"
     gene            1078..2583
                     /gene="cobA"
                     /locus_tag="PKJ17_02110"
     CDS             1078..2583
                     /gene="cobA"
                     /locus_tag="PKJ17_02110"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOD74790.1"
                     /translation="MGKVYLVGAGPGDMKLITMRGLELVRQADVIIYDNLVNKGLLEY
                     ARAGAEIIYAGKKASLHELPQKEINALLAEKARQNSVVVRLKGGDPFIFGRGGEEAEH
                     LAGIGIPFEIVPGVTSAISVPAYAGIPLTHRDHASTVVFITGHEDEKKSASTINWHEL
                     ATGPDTLVFLMGMKNLGKIARRLIFEGKDPATPACVIRSGTLPAQKVVSATLATISEE
                     TRKMQLTPPGVIVVGDVIHLREKLNWFEQKPLFGKKVVVTRSAHQSRRFGELLADRGA
                     EPVYLPTIDIEPIVPNSRLRSAIDRISSYDAIIFTSVNGASIFFAHLRGAGRDARALS
                     GLAIIAIGAATAAHLGVYGIQADLVPEKFTSEGVVSVLEAYGVSGRNFLIPRAEEARD
                     VITRYIRANGGSCSIVPIYRATIPAKAASLPDDLDIVTFTSSSTASNFIMLYGKEILK
                     NRVVASIGPVTTATLTKSGINVDIEAQQSDIPGLLNAIEAYFQPPAKNGKA"
     gene            complement(2597..2776)
                     /locus_tag="PKJ17_02115"
     CDS             complement(2597..2776)
                     /locus_tag="PKJ17_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74791.1"
                     /translation="MEGNRKENVTFLGLQRVPRGEALVLVNNESGSTVTYDPARHNII
                     NMDGYIRSVLASRRR"
     gene            complement(2891..3079)
                     /locus_tag="PKJ17_02120"
     CDS             complement(2891..3079)
                     /locus_tag="PKJ17_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74792.1"
                     /translation="MLMKLSLRKGGTKWVDHNEARMLQRQGQVICVLAIKNEELAEFL
                     PFCSMRNDYCSSCISLPD"
     gene            complement(3575..4372)
                     /locus_tag="PKJ17_02125"
     CDS             complement(3575..4372)
                     /locus_tag="PKJ17_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD74793.1"
                     /translation="MKKDSDEIRALIVDDERLARENLKELLRDQWDVRVVGEARNADE
                     AERMIAALNPNLVFLDIQMPGGSGFDLLARLEDTPSIIFVTAFDQYAIRAFEVNALDY
                     LLKPIEPERLRNALRRAAFRLPSFDPAGEIFSAADQVFLNTGKKASFVSASNIVAIFS
                     ERNYTNVHNIQGERFMVRSSLRDWERRLPPDMFVLLDRSTIINRNHVELWTLHARKAE
                     LKMTGLAEPIELGRAAYTRFKELIVAPKAKGQGAGKGTRGEDEGETA"
     gene            complement(4356..>5310)
                     /locus_tag="PKJ17_02130"
     CDS             complement(4356..>5310)
                     /locus_tag="PKJ17_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOD74794.1"
                     /translation="MDPGGLLVNVNPAFAHLVGREDPAQVMGTSMADHCGADVSSLLL
                     GELRRSGSVRGLEVNLARSDGSRRTGVIYARADRDGEGALIRAQGLLNDITERKEVEE
                     ERHRAQEAQRRFVEAQLETLRYQINPHFLFNVLNSLDALSKTDPERISELVRQLSRYL
                     RSTLSSRESGLTSLEKEMSTIESYLSLEKVRFEEDLVVSVSMPEEIGDALVPELLIQP
                     VVENAVKHGMKTSSLPLRVDVTCRAAGEHVMIEVANTGKWIARDTAADAGKGGVGLGN
                     IRTRLELTYGDRYGLEIGGSDDRVVVTIKIPLRGDKNEKGL"
BASE COUNT         1099 a         1606 c         1439 g         1166 t
ORIGIN      
        1 cccggcgcac tgcatcgcga atgggtccgc cgggcgagga tcgccaaatg ccaccatctg
       61 ctccgagaac cggacggtta cctggctaac gtccttgacg gaaccttccg gcgagaacag
      121 ttccacggag gcgccggcgg catatccgcc ggacccgaac cacaccacca ccgcgaagac
      181 gacgcaaagg acgctcctca tcataccctt atcctcctct ggaaactcga aagaacgaca
      241 gacgatcctc gttcatccat caatactata agaaaggatg acccttttca agaaagaaac
      301 cgcccgacgg ggacgaggaa gggagaggaa gaataggact tataggagaa taggacctat
      361 aggacatatc ctcctattcg tcctatctct tctcccatcc cctctcccga ccgctgctca
      421 ctccagccct ttccccgtgc tggacatgct cagtgtgcag tgttttatgc ccttgatcgc
      481 tcttagcatc tccgccaggc ggcgcacctc atcgggcttt cccctgacgg cgactatctc
      541 gagacaattg ttgtggtcga gatggacgtg ctgcgtggat attatggtgt gctggtaatc
      601 atgctgaagg tccgtcacct tgttcaagag ctcccggtga tgatggtcgt atatgagtgt
      661 tatcgccccg gcaacttctt ctccaaccac ccattccttt gctatgagtt cctgccgtat
      721 cagatcccgg aacgcctcgg accggttgga gtagctcttc tccctgataa gctcatcgaa
      781 cttcttcaac aggtccccgt caagcgaaac cccaaaccgc actaattgag acatggtcac
      841 ccccttgtgg cacgttcaag atgaagagta gcacaaggag aattggctgt caattggttc
      901 gagggttcga gggaaaaacc gcggaggggg gtggttgtca agacaatcgt tgactagcgc
      961 agatgtccgt tgcccttttt tgtctttcac tcttgaaccc ttgaactctt gaacccttga
     1021 actcttgagc ctcatttatc ctttgagaaa aaagcgcaac ctccgtataa tgttcgcatg
     1081 ggcaaggtat atctcgtcgg cgcgggcccc ggggacatga agcttattac catgagaggt
     1141 ctcgagcttg tccgccaggc cgacgtcatc atctacgaca acctcgtgaa caagggcctt
     1201 cttgagtacg cccgcgccgg cgcggagatc atatacgcgg gcaagaaggc gtccctgcac
     1261 gaactccccc agaaagagat caatgccctt ctggcggaaa aggcgcggca gaacagcgtg
     1321 gtcgttcgtt tgaagggcgg cgatcccttt atcttcggcc ggggaggcga agaagcggag
     1381 caccttgccg ggatcggaat tcccttcgag atcgtcccgg gtgttacgtc ggctatctcc
     1441 gtccccgcct acgccggtat acccctcacg cacagagatc acgcctccac cgttgtcttc
     1501 ataacaggcc acgaggatga gaagaaatcc gcgtctacca tcaactggca cgaactggca
     1561 acggggccgg atactctcgt tttcctcatg ggtatgaaga acctcgggaa gatcgcaaga
     1621 cggctcatct ttgaaggcaa ggacccggcc accccggctt gcgtcatccg gtcgggtacc
     1681 ctgccggctc agaaggtcgt ttcggcaacg cttgccacga tctccgagga gacgcgcaag
     1741 atgcagctca ctccaccggg ggttattgtg gtcggcgacg ttatccacct gagggaaaag
     1801 ctgaactggt tcgagcagaa acctctcttc gggaaaaagg tggtggtgac gcggtcggca
     1861 caccagtccc gcaggttcgg tgaactcctc gcggaccggg gtgccgaacc cgtctatctc
     1921 ccgaccatcg atatcgagcc catcgtcccc aactcccggc ttcgctccgc gatagacagg
     1981 atatcctcat atgacgccat catcttcacg agcgtcaatg gggcctccat attcttcgcg
     2041 catctcaggg gagcggggag ggacgcccgg gccctgagcg gcctcgccat tatcgccata
     2101 ggagccgcaa cggccgcgca tcttggcgtc tacggcatac aggctgacct tgttccggaa
     2161 aagttcacgt ccgagggagt tgtctccgtc cttgaggcct atggcgtttc gggaaggaat
     2221 tttctcatcc cccgtgctga agaagcccgc gatgtgatca ctcgatacat aagggcaaat
     2281 ggaggaagtt gcagcatcgt gcccatttac agggcaacga tcccggcgaa ggccgcatcc
     2341 ctacccgacg atctcgacat cgtcactttc acgagttctt ccacggcaag caatttcatt
     2401 atgctctacg gaaaggagat cttgaagaac agggtcgtcg cctcaatagg ccccgttacg
     2461 acggcaacct tgacaaagtc gggtatcaat gtcgatatcg aagcccagca atccgatatc
     2521 cccggattgc tgaacgctat cgaggcctat ttccagcctc ccgcaaaaaa cggtaaggct
     2581 taaggcaagc ctctcgtcac ctgcgccttg acgccaggac ggaacggatg tatccgtcca
     2641 tgttgataat gttgtgcctg gcggggtcat atgtgacggt gctcccgctt tcgttgttca
     2701 ccagaacgag tgcttctccc cgcggcaccc tctgaagccc gaggaatgtg acgttctcct
     2761 ttcgattacc ttccatcgcc gtcctccctt atctggggac ctgcggttcc gtcgcgggca
     2821 ctcgaacatg tcgtctccgc cgcataacag ttcagaaagc cgcaggccac atccgggcta
     2881 cgcagaccct ctagtcgggc aacgagatac aggaactgca gtagtcgttc ctcatgctgc
     2941 agaaaggcag aaactcggca agctcttcgt tctttatggc gagaacgcag atgacctgtc
     3001 cctgcctctg gagcatcctg gcttcgttgt ggtccaccca tttcgtcccg cccttcctta
     3061 aagacaattt catgagcata tcgtcctccc tcgttgttgt aatgataggg tatcctatcc
     3121 agtctgccgg agggtccgag aggctatgtg ccgaatcgac ccgccggagt tgccgacaag
     3181 ccatccagag gtcattcaag gtctccggga ctgtgttagc gggtgactgc ttttggggaa
     3241 gccggggcac tcactccccc ccgcaggtga atgtgccccg acttggtggg atgatgaaac
     3301 attggggttt gcggatacaa ttctaccgga ttatcggggc ccgcgtaaga cagagggaca
     3361 gaccagacct gattagtgac gaaccacgga acagtcggtg atagcgagga caaggaggcc
     3421 gggacttgtg cagacacgat ggtgttggca tgtcccggcc tgttggaatg gtgcgactac
     3481 aggggtttgt ggaaatggat tttatatgac aagaaaggag ggtgccagta ttattgtacg
     3541 atcgtgcccg atgaagtgct caaacagcaa tcctttacgc cgtctcccct tcatcttctc
     3601 ccctcgtacc cttcccggct ccctgacctt tcgctttcgg ggcgacgatc agttccttga
     3661 accgggtgta ggccgccctg cccagctcga tcggctccgc caggcccgtc atcttgagct
     3721 ccgctttcct ggcgtgaagg gtccacagct cgacgtggtt cctgttgatg atcgttgaac
     3781 gatcgaggag gacgaacatg tccggaggca ggcgcctctc ccagtctctc agcgacgacc
     3841 ggaccatgaa gcgctctccc tggatgttgt ggacattggt atagttcctc tcggagaaaa
     3901 tggccacgat attgctcgcg gacacaaaag acgccttctt tccggtattg aggaagacct
     3961 gatcggcagc ggagaagatc tctcccgcgg gatcgaagga aggcagacgg aacgccgccc
     4021 ttctcaacgc gttcctgaga cgttccggtt cgatgggttt caggaggtaa tccaacgcgt
     4081 tgacctcgaa cgcccgtatg gcgtactggt cgaaggccgt aacgaagatg atggaaggcg
     4141 tatcttccag gcgcgcgagg agatcgaacc ccgatcctcc cggcatctgg atgtccagga
     4201 ataccaggtt gggattgagg gcggcgatca tcctttccgc ctcgtccgcg ttccttgcct
     4261 cgccgaccac cctgacgtcc cactggtccc tcagcagttc cttcaggttc tcccgtgcaa
     4321 gccgttcgtc gtcgacgatg agtgcccgga tctcgtcaga gtcctttttc attcttgtct
     4381 cctcttagag ggattttgat ggtgaccacc accctgtcgt ctgacccgcc gatctcaagg
     4441 ccatatcggt caccataggt aagctcaagc cgtgtgcgta tgttcccaag cccgacaccg
     4501 ccctttcctg catccgctgc ggtatcccgg gcgatccact tccccgtgtt cgcgacctcg
     4561 atcatcacgt gctcgcctgc cgccctgcac gtcacatcaa cccgaagagg aagggacgat
     4621 gtcttcatcc cgtgcttcac ggcattttcc acgacgggct gtatgagcag ttcgggaacc
     4681 agggcgtccc ctatctcctc gggcatgctg acagagacga ccagatcctc ctcgaaccgg
     4741 accttctcca ggctgagata gctttcgatg gtgctcatct ccttttcgag ggatgtaagt
     4801 cccgactccc gcgacgagag agtggagcgc aggtaccggg agagctgccg cacaagttcg
     4861 ctgatgcgtt ccgggtctgt cttcgaaagg gcatccaggg aattgaggac attgaaaagg
     4921 aaatggggat tgatctggta cctcaacgtc tccagttggg cctcaacgaa ccttcgctgg
     4981 gcctcctgtg ctctgtggcg ctcctcttcg acctctttcc tttcggtgat gtcattgagc
     5041 agaccctggg ccctgatcag cgccccttcc ccgtcacggt ccgccctcgc gtatatgaca
     5101 cccgtccttc ttgacccgtc gctccgcgcc aggttgacct ccagacccct gacgctgccc
     5161 gacctccgca attccccgag aagaagggaa ctcacatcag cgccgcaatg gtcggccatg
     5221 ctggttccca tcacctgcgc cggatcttcg cggccaacca ggtgggcaaa ggccgggttg
     5281 acgttcacga gaagaccacc ggggtccatg
//
LOCUS       DAOPOA010000059         5294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17018_length_5294_cov_2.901890, whole genome shotgun sequence.
ACCESSION   DAOPOA010000059 DAOPOA010000000
VERSION     DAOPOA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17018_length_5294_cov_2.901890"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PKJ17_02135"
     CDS             complement(<1..897)
                     /locus_tag="PKJ17_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HOD74795.1"
                     /translation="MIYTITLDPLHERVVGVEELVYDDVNCVVDETRRPGGRGIDVSR
                     VVRELGGDSVALGFAGGYTGLELTDMLDREGIVTDFVRIGQETRSSITIYQRKKKIRT
                     LLCTSSPVIREDEAASFFDKVKGIPGGSCVVLSGTTAGLCDAVFAEFISILKEKGVRT
                     FLDSDHKALKLGVNAGPFLIKPNIFELNRLAATKAKEVKEIAEAVKHFRDGVEYIVVS
                     MGARGALGFSKEGNYHVTPPKVKVRNSLGAGDSFVGGVVSVMSDSGSFEEALRVGVAC
                     GTATVLAASGGLCRKTDVDSIKK"
     gene            complement(1006..2262)
                     /gene="hisS"
                     /locus_tag="PKJ17_02140"
     CDS             complement(1006..2262)
                     /gene="hisS"
                     /locus_tag="PKJ17_02140"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOD74796.1"
                     /translation="MEKIRTLRGFRDIFGEELDKFRRIESVFRKYSGLLGFREIELPV
                     LEKTELFVRSIGDTTDIVEKEMFTFTDVGGDSLTMRPEATAGMVRSYLQEGLYATERM
                     TKVSSIGPMFRHERPQKGRYREFHQIDVEVFGVKEALVDAELIFMIRMILDELGVGRY
                     RIEVNSVGCKTCRESFRRVLIDYFETRKDQLCEDCLRRLERNPLRIFDCKNEQCIDVT
                     GDSPLLFDSLCGECRDHFRLFEGYLSDFGIGVEINKRLVRGLDYYTKTVFEVTSEDLG
                     AQKAFIAGGRYDNLVEEMGGPATAGIGFAIGVERLAMLVPVSAEKGDRRCFFACVGDR
                     ARAHLVSFLKACVSAGITLKHAYDAKSLKAQMRYADNLKCDFVLILGDDEIDRGIITL
                     RNMADKSQRELPLDPKKVAQELVVVA"
     gene            2394..2774
                     /locus_tag="PKJ17_02145"
     CDS             2394..2774
                     /locus_tag="PKJ17_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOD74797.1"
                     /translation="MDDNRQKMIEHSEHPRNRGGMTNPDGIGEIGNASTDVHLRVFIR
                     VDENLITDVRYEIKDCPTSLACASIMTELAIGKDLDEAVTIEGEDIANALGGLPGNML
                     YCSNRAARALQEAIIDHVYRRRGA"
     gene            complement(2864..4117)
                     /locus_tag="PKJ17_02150"
     CDS             complement(2864..4117)
                     /locus_tag="PKJ17_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD74798.1"
                     /translation="MPHREDADALKEKYYHSMGAILTEEGDLEEAVVFFQKAIDIRET
                     PYAWYGLARTLYAAGDVAGAVGAMSRAIKLAPGAADYYHERAAFLEALGRPDLAAGDV
                     AKAVSLDRNYERVDEIRWAARVVEGSFFPPVPFVEDAARIRSGGLRRLLEGEGDADVF
                     RKPSCPVLCCPAYCCHFTGRLLLHGVTIGPWKLQGLRGHFREKGLREEDLLDVFPVAE
                     AGHAESLFPPQDVMKRGGVASVTFPRQGTSPLGRDLAGDIPKGKDYRTLMWIGEDARP
                     CVFLSGGRCSLYDVGDEASLDPCASFLCMTGFVFVVLRSLGLMDSEAMGARSMAELNG
                     IAVEALIILARDVYGSDEALASSKTLADELQTAVEGDLAGDDVLRDAAMERYRLLKTR
                     HEDLIGELAASAGKRIARLFAGGVG"
     gene            4230..>5294
                     /locus_tag="PKJ17_02155"
     CDS             4230..>5294
                     /locus_tag="PKJ17_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOD74799.1"
                     /translation="MPSPSERHAAFRWIVVTVAFAAFMSKLDSYIVNISLPTIAARFN
                     VTTAEVSWVVLVYLLVSTSTLLLFGKLGDRFGLRKLFFLGYLLFTIGSLLCGTALSLW
                     MLIGSRFLQGIGCAMLTAIAFAVIPRFLPHEVAGWAFGIAATGAALGIAVGAPAGGII
                     TGFLSWRWVFLVNVPFGIFAMYAVNRYIPDEDGSHARNLRKAGFDILGAGLSFAGLAF
                     LVYGLNTGREFGWTSPRIMGCFAVAFVLLAAFLIREIKCEEPLLDLRLFKKFRFSAAV
                     ASTLFAYMLLAGNSFLLPFYLELGKGLPTVTVGLVLMIYSVIYMVVGPWAGRISDRVP
                     PSILCGFAMLSAGLCSFVFAF"
BASE COUNT         1055 a         1666 c         1400 g         1173 t
ORIGIN      
        1 ttttttgatg gaatcaacat ccgtttttct gcacagaccc ccggaagcgg caaggaccgt
       61 cgccgtaccg cacgccacgc cgacccttaa agcctcttca aagctgcccg agtcgctcat
      121 caccgagact acgcctccca cgaacgaatc accggccccc agcgaattac gcacttttac
      181 cttcggcggc gtgacatgat agttgccttc ctttgagaag ccgagagcac cccgcgcacc
      241 catcgataca actatatact cgacaccatc gcggaaatgc ttaacggctt ccgcgatctc
      301 tttcacctct tttgcctttg tcgccgccag tctgttcagc tcgaagatat tgggcttgat
      361 caggaaggga cccgcattga ctcccagttt gagggcctta tgatcggaat cgagaaaggt
      421 cctgacgcct ttttccttga gaatgcttat gaactccgca aagacagcgt cacagagacc
      481 ggccgtcgtg ccgcttaaga ccacgcaact gccaccgggg atccccttca ccttgtcgaa
      541 gaaggaagcg gcttcatctt cacggatcac cggactcgac gtgcacagta atgtccggat
      601 cttttttttc cgctggtaga tcgttatact ggaacgtgtt tcctggccga ttcggacaaa
      661 atcggtgacg atgccttccc tgtcaagcat gtccgtgagc tcgagtccgg tgtatccacc
      721 ggcgaaaccc agcgccacac tgtctccccc aagctccctg acaacccgcg atacgtcgat
      781 ccccctgccg ccgggcctcc tggtctcgtc aacaacgcag ttgacatcgt cgtacacgag
      841 ttcttccacc ccgacgaccc tttcgtgaag aggatccaat gtgatggtgt atatcattga
      901 tcattgagat tgtattagat aaagaaaaaa atggctagag atttttttgt ctctgtctcc
      961 gcgttcagcg cttgcggttt gtgacgtgcg accccggacg tgacgctagg ccacgaccac
     1021 cagctcctgc gccaccttct tcgggtcgag agggagttcc cgctggctct tgtccgccat
     1081 attcctgagg gttatgatac ccctgtcgat ctcgtcatct ccgaggataa ggacaaagtc
     1141 gcacttcagg ttatcggcgt agcgcatctg ggctttgagg gatttggcgt cataggcgtg
     1201 tttcagggtt atccccgcag agacacaggc cttcaggaac gaaacgaggt gagcccgggc
     1261 ccggtcgccg acgcaagcga agaaacaccg tctgtcaccc ttttcggcgg agaccggaac
     1321 gagcatggcc aaccgttcga caccgatcgc gaacccgatg ccggccgtgg ccggtccgcc
     1381 catctcttcg acaaggttgt cgtatctgcc tccagcgata aaagccttct gcgcgccgag
     1441 gtcctccgac gtaacctcga agaccgtctt tgtgtaatag tcaaggcctc tcaccaggcg
     1501 cttgttgatc tcgacaccga taccgaagtc gctgagatat ccctcgaaga gacggaagtg
     1561 gtctctgcat tctccgcaga gagaatcgaa aaggaggggg gaatcgccgg taacatcgat
     1621 gcattgctcg ttcttgcagt cgaagatcct caaggggttc ctctcgagcc tcctcaaaca
     1681 atcctcgcaa agctgatcct ttctcgtctc gaaataatct atgagcacgc ggcggaagga
     1741 ttcccggcag gtcttgcatc caacactgtt cacctcgatc ctgtaacgtc ctacgcccag
     1801 ttcgtcgagg atcatgcgga tcatgaatat cagttcggca tcgacgaggg cctctttcac
     1861 tccgaacacc tccacgtcga tctggtgaaa ttcccggtag cgccccttct ggggcctctc
     1921 gtggcgaaac atgggaccga tggagctgac cttcgtcatt ctttccgttg cgtaaaggcc
     1981 ttcctgcaaa taggaacgca ccatgcccgc ggtggcctcc ggtcgcatgg tcagcgaatc
     2041 gccgcccacg tcggtgaagg tgaacatctc tttttcaacg atatcagtgg tgtctccgat
     2101 gctccggacg aagagctccg tcttctcgag aacgggcagt tcgatctccc gaaaaccgag
     2161 aaggccgctg tatttccgaa acacgctttc tatacgcctg aacttgtcaa gctcttcccc
     2221 gaagatgtcg cgaaatcccc gtagagtcct tatcttttcc atcgatgctc ccgtatgttt
     2281 gttgccttta tcaatcattt aggtatcaca aaacaaagag caattgaaag tgatttttgc
     2341 ccgtttatgg tatagtaggg accagtttca cggaaggagg ccgggcgcca ggcatggacg
     2401 acaacaggca aaagatgatc gaacactccg agcatccgcg caaccggggc ggcatgacga
     2461 accccgatgg catcggtgag atcggaaacg cgtcaaccga tgtccacctg cgggtattca
     2521 tcagggtgga cgagaacctg ataacggacg tcaggtacga gatcaaagac tgcccgacat
     2581 ctctcgcctg cgcctccatt atgacggagc ttgccatcgg caaggacctc gacgaggccg
     2641 tcacaataga aggtgaagac atcgccaatg ccctgggcgg gttgcccggc aacatgctct
     2701 actgttcaaa ccgtgcggca agggcgcttc aggaagcgat catcgaccac gtctacagaa
     2761 gaagaggcgc ttgaacagct cgaaccgctt gagctgcttg agcgttgaaa agaaattgac
     2821 agatctcatc tctttacctt ttcgcgcggg tctcgctgac atctcatccc accccaccgg
     2881 cgaaaagccg ggcgatcctc tttcctgccg aggcagcgag ttcgcctatc aggtcctcgt
     2941 gccgggtctt cagcaggcgg taccgttcca tggccgcgtc ccgcaggacg tcatcgcccg
     3001 caagatcccc ttcgacagcg gtctgcagtt catcggccaa ggtctttgac gatgccaggg
     3061 cctcgtcact gccatagacg tcgcgggcca ggatgattag ggcctcgacg gctatcccgt
     3121 tgagttccgc cattgacctt gcgcccatcg cctcgctgtc catgagaccc agggacctca
     3181 agaccacgaa gacaaaaccg gtcatgcaga gaaaggacgc gcacggatcg agacttgcct
     3241 cgtcgccgac gtcatagagc gaacaccgac caccggaaag aaagacgcag ggtctggcat
     3301 cctcccctat ccacatgagg gtccggtagt ctttgccctt cggtatgtcc ccggcgaggt
     3361 cccggcccag cggggatgtc ccctgccgcg gaaatgtgac ggatgcgacc ccgccgcgct
     3421 tcatgacgtc ctgcggcggg aaaaggctct ccgcgtgccc cgcttcggcc acgggaaaga
     3481 catccaggag atcttcctcc cggagaccct tttcccgaaa atgcccgcgc agaccctgaa
     3541 gtttccaggg gcctatcgtc accccgtgca gaaggagtct accggtaaaa tgacagcaat
     3601 atgccggaca gcacaggacg gggcaggacg gcttgcgaaa gacatcggca tccccttccc
     3661 cttcgaggag gcgccgcagc cccccggacc tgatccgggc ggcatcctca acgaaaggga
     3721 ccggtgggaa gaacgatcct tccacgacgc gcgcggccca cctgatctcg tccacccgct
     3781 cgtagttcct gtcgagggaa accgccttcg ccacgtcgcc ggcggccaga tccggcctgc
     3841 cgagcgcttc caggaacgcc gcccgctcat gatagtaatc ggccgcgcct ggagccagct
     3901 tgatggcccg cgacatcgca ccgacggcgc ccgcgacatc gccggcggcg taaagagtcc
     3961 gagccagtcc gtaccacgcg tagggcgttt cacggatatc gatggccttc tgaaagaaca
     4021 cgacggcctc ttccaggtcg ccctcttccg tgaggatggc tcccatggag tgatagtact
     4081 tttctttcaa cgcgtcggcg tcttcacgat gcggcacgga tcggtcctcc ccgcacatgt
     4141 taccatgccc cggacgatac ttccacacct cgccgccctg gaacaaatgt gttgaaaaat
     4201 ggagtcaatt cgttcagaat ggttgtcgta tgccttcccc atccgaacgg cacgccgctt
     4261 tccgttggat cgttgttacc gtggccttcg ccgctttcat gtcaaagctc gacagctaca
     4321 tcgtcaacat ttccctgccc acgatagcgg cacgtttcaa cgtgaccaca gcggaggttt
     4381 cctgggtcgt cctcgtctat cttctcgtct cgacgagcac gctgcttctc ttcggcaaac
     4441 tgggggaccg gttcggcctc aggaagctct tttttcttgg ctatcttctc ttcaccatcg
     4501 ggtcgctcct ttgcgggacc gccctttccc tttggatgct cataggctcc cgcttccttc
     4561 aggggatcgg ctgtgccatg ctcaccgcca tcgcctttgc ggtcataccc aggttcctgc
     4621 cacatgaggt tgccggctgg gccttcggca tcgcggccac gggggccgct ctcggcatag
     4681 cggtcggcgc gccggcgggc ggtatcatca ccggttttct ctcctggcga tgggtcttcc
     4741 tcgtcaatgt acccttcggc atattcgcca tgtatgccgt caacagatac atccccgacg
     4801 aggacgggtc tcatgcccgg aacctcagga aggcggggtt tgatatactg ggtgccggct
     4861 taagctttgc cggtctcgct ttcctggtct acgggctcaa cacgggcagg gaatttggct
     4921 ggacatctcc gaggatcatg ggatgctttg ccgtggcctt cgttctgctc gccgctttcc
     4981 tcattcggga gatcaagtgc gaggagccgc tccttgacct caggcttttc aagaagttcc
     5041 gcttcagcgc cgccgttgcc tccactctct tcgcttacat gcttctggcg ggaaattcct
     5101 tccttctgcc cttctatctc gaactgggca agggacttcc gacagtgacc gtcgggctcg
     5161 tcctcatgat ctactccgtg atctacatgg tcgtcggccc ctgggccggc cggatttccg
     5221 acagggtccc ccccagcata ctctgcggat tcgccatgct ttccgccggc ctttgctctt
     5281 ttgtcttcgc tttc
//
LOCUS       DAOPOA010000060         5280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17076_length_5280_cov_2.957895, whole genome shotgun sequence.
ACCESSION   DAOPOA010000060 DAOPOA010000000
VERSION     DAOPOA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17076_length_5280_cov_2.957895"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PKJ17_02160"
     CDS             complement(<1..221)
                     /locus_tag="PKJ17_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74800.1"
                     /translation="MKRIAARIGERVVSLSNLDKDLYPSYGFTKADVLEYYRRISPCI
                     LPHLRDRALTLKRYPDGIGGDHFFEKRCPA"
     gene            complement(218..1009)
                     /locus_tag="PKJ17_02165"
     CDS             complement(218..1009)
                     /locus_tag="PKJ17_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOD74801.1"
                     /translation="MALQSIWTGTITFSLVAIPVRLVKAVRPDRISFRMLHAKDYSPL
                     IRAFYCPDEDRMVPPGEIVRGYEVGPEQYIEVTEDELDSVSPERSRTIEIMDFVDVSD
                     VDPLYYDRPYYLAPGRGGEKAYGLLVQVMLRTNRGGIAKFVLGEREHLVLLRVSGAAL
                     SLVTLHYSDEVVPAAETVPEEKEEGGVEEEKDRMARTIRRMEARFDPARYRDDRRRKI
                     MALLGRKEEVEAPVEAPTSFEDTQEGPGDLVAVLQESVRKLKGVR"
     gene            complement(1032..1802)
                     /locus_tag="PKJ17_02170"
     CDS             complement(1032..1802)
                     /locus_tag="PKJ17_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOD74802.1"
                     /translation="MVSRALWKGQIHFGDFDVPVKLHTAVREQRIQFHLLHSRDHVRL
                     RQHMLCAYEKVPVPREEQVRGFEVGEGRYIIVDDDELERLEPEGNRTIEIHDFVRGGQ
                     IDPLYFDRVYYLAPDVMLRGYRALAEALGETGMEGICTWTMRKRSYLGSLAAKGKAMR
                     LITLRHADEVIDAGSLDLPVTGLSDRELEAGVNLVTALSAPFQPEKFKNDHQKKLREL
                     IDRKAAGEKISILRPRQRRATPPDRLLETLEASLKKAV"
     gene            1989..3155
                     /locus_tag="PKJ17_02175"
     CDS             1989..3155
                     /locus_tag="PKJ17_02175"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HOD74803.1"
                     /translation="MSRISNEPDRLTEAPLSPGPGRDCPKDDAFARETDDNLAERRPE
                     EVRYRFAFTAITGEYFRIWIVNLFLTVITLGVYAAWAKVRSRRYIWANINLAGHPFTF
                     HGRPAAILRGNMIIAALFIAYVSMKRFEPSFAGILFILFTFIFPFLVCKSLRFNAHNT
                     SYRNIRFHFRGTLRESYETYLLFAGIVPFTLFLFFPAWQFYKKRFFLGNLAYGTTGSS
                     FAGRKGPFYKAYFFAVVLGIIVFIALSSLAAVGVHRLTTGSAVEWGPLKVIYSLFFTY
                     LLGATILYTLQALVYSMTMNHCWEKTRLGEVRFVSTLKARRLLFIRITNMAAILCSLG
                     LLIPWAKIRRTRYILDNLAVVTSRGFDGFASRDASDTTAVGDAATDFVGIEIGL"
     gene            3152..4201
                     /locus_tag="PKJ17_02180"
     CDS             3152..4201
                     /locus_tag="PKJ17_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOD74804.1"
                     /translation="MIRFDAHFFDGRTSRALPVTVDFDGVTLAFGNEMDGTAVRASII
                     DCSIDPAVGRTKRSIRLPGGPLLQTYDHNAVRTMERSLGVNRSSGLVHFLESRWKMVA
                     VCMVCLALAIFALVYYAIPHASVRVARIIPPEVMASASTNTLKIFDARFLGPSGLTAE
                     RQDEIRGLFEDVARNIDPGGATGYRLEFRTGKTIGANAFALPSGIIIVTDELVSLSES
                     DDELAGIFAHEIAHVKNRHALRSILQSTGVFFLITLLSGDVTSITSFAAFLPTLLIES
                     GYSREFEREADQAAASFLIAGDKGTVPFRKMLEKLDASQPGALKLGVFSDHPETAKRI
                     EHLKELQRSAGGGSR"
     gene            4283..5179
                     /locus_tag="PKJ17_02185"
     CDS             4283..5179
                     /locus_tag="PKJ17_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOD74805.1"
                     /translation="MNKTIRRSLPVILILLLFTVVIPARARAEGEKHFLWQAKTQTAT
                     VYLLGSVHFMKRDAYPLSPVIEKAFAGSDTIAVEANINDIGQETLAKLRAEGFYTGND
                     SIANHVSRETYTYITAEAARLGFPVASLDRQRPWFLAMTMSSIELMKAGYNPHYGIDK
                     YFLTRAAGRKKLVELETIDYQIDLLARLPDKEQESFLLYALKDLSSLVQQVDRLMAAW
                     TTGDSDLIAELLEKSVGEDESLKVIHRKILTERNRNMAKKIALHLGSSGCVFVVVGAA
                     HLVGDKGIVELLRKEGFTVEQL"
     gene            5199..>5280
                     /locus_tag="PKJ17_02190"
     CDS             5199..>5280
                     /locus_tag="PKJ17_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOD74806.1"
                     /translation="MTRIVLLRHGESQWNLENRFTGWTDID"
BASE COUNT         1044 a         1685 c         1454 g         1097 t
ORIGIN      
        1 gcgggacagc gtttctcaaa aaaatgatcg cccccgatgc cgtcgggata gcgcttcagc
       61 gtgagggccc tgtccctgag gtggggcagg atgcatggtg agatgcggcg atagtactcg
      121 agaacatcgg ccttcgtgaa accatacgac ggatagaggt ctttatccag gttggaaagg
      181 gacactactc tttcgccgat gcgggccgcg atcctcttca tcggacacct ttaagcttgc
      241 gcacgctctc ctgaaggacg gccaccaggt cgccggggcc ctcctgcgta tcctcgaaag
      301 aggtaggtgc ctcgacaggg gcctcgacct cctccttcct tccgagaagg gccatgatct
      361 tcctcctgcg gtcgtcgcgg tacctggcgg gatcgaatct ggcctccatc cggcggatgg
      421 ttcgggccat ccggtccttc tcttcctcca ccccgccctc ttctttttct tcgggcaccg
      481 tttccgccgc gggcacgacc tcatcgctgt agtgaagggt aacgagtgaa agggccgcac
      541 ccgaaaccct caggaggacg agatgttccc tctcgccaag aacgaacttc gcgatgccgc
      601 ccctgttcgt ccggagcatc acctggacga gaagtccgta cgccttttcc cctcccctgc
      661 caggagcgag atagtagggc cggtcataat acaggggatc aacgtcgctc acgtcgacaa
      721 agtccattat ctcgatggtg cggctgcgct cgggcgaaac ggaatcgagc tcatcttcgg
      781 tcacctcgat gtactgttcc ggtcccacct cgtatccccg cacgatctcc ccgggaggaa
      841 ccatcctgtc ctcgtccggg cagtagaacg cccttatgag aggggaatag tcctttgcgt
      901 gaagcatgcg aaaggagatg cggtcgggcc ggacggcctt caccaaccgc accggtattg
      961 ccacgaggct gaaagtgatc gttcccgtcc agatggattg cagtgccatg cggcctccct
     1021 tctacccgtt ctcacaccgc ctttttcagg ctcgcttcca gtgtttccag gagcctgtcc
     1081 ggtggggtgg ccctgcgttg tctggggcgc aggatggata tcttctctcc cgcggctttt
     1141 ctgtcgatca actcccggag tttcttctga tggtcatttt tgaacttctc cggctgaaaa
     1201 ggggctgaca gggcagtgac cagattgacc cccgcctcga gttccctgtc ggacaagccg
     1261 gtcacgggca gatcgagaga tcccgcgtct atgacctcat ccgcatggcg cagagtgatc
     1321 agcctcatgg ctttcccctt tgccgccagg gatccaaggt aggatcgttt cctcatggtc
     1381 caggtacata taccctccat acccgtttct ccgagtgctt cggcgagggc ccggtatccc
     1441 cgcagcatca cgtcgggcgc cagataatag acccggtcga agtaaagcgg gtctatctgc
     1501 ccgcccctca cgaaatcatg gatctctatc gtcctgttgc cctccggctc aaggcgctcc
     1561 agttcatcgt cgtcaacgat gatgtacctc ccctcaccca cttcaaaacc acggacctgc
     1621 tcctcccggg gcacgggcac cttttcatag gcacacagca tgtgctgcct gagccgcaca
     1681 tgatcgcgac tgtgaaggag atgaaattgt atccgctgct ccctgaccgc ggtgtgcagc
     1741 tttaccggaa cgtcgaaatc gccgaaatgg atctgtcctt tccagagggc ccgcgatacc
     1801 atgggtccac ctcccttatc tagaacagat acccacgaaa ggtctttctg tcaaactctc
     1861 cggcgcatgc cgggacactc gggacgcggt atcgggccaa tcttcggcgc accgagaaaa
     1921 tcctttttaa ttccgtcacc cacgtttata ataaacggac cgataatacc ggaggaacgt
     1981 acccgagggt gagccgcatc tcgaatgaac ctgatcgcct gaccgaggcc ccattgtcgc
     2041 cgggcccggg gcgggattgt ccgaaagacg acgccttcgc gcgggaaacg gacgacaacc
     2101 tggccgagag gaggcctgaa gaagtgcggt atcgcttcgc cttcaccgcc ataacgggtg
     2161 agtatttcag gatctggatc gtaaacctct tcctcaccgt catcaccctg ggggtgtacg
     2221 ccgcctgggc caaggtgcgc agccgacggt acatatgggc caatatcaac ctggcaggtc
     2281 accccttcac ctttcacggc agaccggcgg cgatcctgag ggggaacatg atcatcgcgg
     2341 cgctcttcat cgcctacgtc tcgatgaaac ggttcgagcc ctccttcgcc ggcattctgt
     2401 tcatcctctt taccttcatc ttccccttcc ttgtctgcaa gtccctgcgt ttcaacgctc
     2461 acaacacctc ttacaggaac atccgctttc actttcgcgg caccctcagg gaaagctacg
     2521 agacgtatct tctctttgcc ggcatcgtcc cctttaccct cttcctcttc ttcccggcct
     2581 ggcagttcta caaaaaaagg ttcttcctgg gcaatctggc atacggcacg acgggaagct
     2641 ccttcgccgg acgcaaaggg cccttctaca aggcttactt cttcgccgtg gtcctgggga
     2701 tcatcgtttt catcgctctg tcctcccttg ccgccgtcgg cgtccaccgc cttaccacgg
     2761 gttccgccgt agagtgggga ccgctcaagg tcatctattc cctcttcttc acctacctcc
     2821 tcggggcgac catcctctac acccttcagg cgctcgtcta ctccatgacg atgaaccact
     2881 gctgggagaa gacgcgcctc ggagaggtcc ggttcgtgag caccctcaag gcgcgaagac
     2941 tcctttttat aaggataacc aacatggcgg ccatcctctg ctcgctgggc cttctcatcc
     3001 cctgggcaaa gatacgccgc acccgctaca tccttgacaa cctcgccgtg gtcacctccc
     3061 gcggcttcga cggtttcgct tcccgcgatg cctcggatac gacggccgtt ggcgatgccg
     3121 ctacggattt cgtcggtata gagataggcc tatgatacgc ttcgacgctc atttcttcga
     3181 tggaaggaca tcccgggccc tgcccgtgac cgtcgatttc gacggagtca cccttgcctt
     3241 tgggaacgag atggacggca ccgcggtgag agcatcgatc atcgactgtt ccatcgaccc
     3301 tgccgtcggc cggacaaagc ggtccattcg gctgcccgga ggccccctgc ttcaaacata
     3361 cgaccacaac gcggtgcgca cgatggagcg cagcctgggc gtgaaccgct cctcaggtct
     3421 cgtccacttc ctcgagtccc gctggaagat ggtggcggtc tgcatggtat gtctcgccct
     3481 cgccatcttc gccctcgtct actacgccat cccgcacgcg tcggtgcggg tggcccggat
     3541 catccccccg gaggtcatgg cctcggccag cacgaacacg ctcaagatct tcgacgcgcg
     3601 ttttctggga ccgagcggat tgaccgcaga gcggcaggat gagatcaggg gccttttcga
     3661 agacgtcgcc cgaaacatcg accccggcgg ggcgaccggc tacaggctgg aattccggac
     3721 cgggaagacg atcggcgcca acgccttcgc gctcccttcg gggatcatca tcgttacgga
     3781 tgagctcgtc tccctctctg aaagcgatga cgaactggcc ggcattttcg cccacgagat
     3841 cgcccacgtg aagaaccgac atgccctgcg aagcattctg cagagcaccg gcgtcttctt
     3901 cctcatcaca cttctttcgg gagacgtgac atcgataacc agctttgccg ctttccttcc
     3961 caccttgctc atagaaagcg gctactcgcg ggaattcgag agggaggccg accaggcggc
     4021 cgcctctttt ctcatcgcgg gggacaaggg gaccgttccc ttccggaaga tgctggagaa
     4081 gctcgacgcg agccagcccg gtgcgctcaa gctgggcgtc ttctccgatc atcccgaaac
     4141 ggcgaagcgc atcgagcatc tgaaagaact gcaaaggtcg gcgggcggag gatcgagata
     4201 gccgcggact ccgcgcatcc tttgtaaggc cgccccgttt ctgctataat ggtcgggcat
     4261 gccgacctta cgagaggaca ccatgaataa gacgatccgc cggtcactgc ccgtcatcct
     4321 catccttctc ctcttcaccg tcgtcatccc ggcccgcgcg cgggcggaag gagagaagca
     4381 tttcctctgg caggcgaaga ctcagacggc cacggtgtac cttctcggtt ccgtccactt
     4441 catgaagaga gacgcctatc ccttgagccc ggtgatcgag aaggccttcg ccgggtccga
     4501 caccatagcc gttgaggcaa acatcaacga tataggacag gaaaccctgg cgaagctccg
     4561 cgccgagggt ttctacacgg gaaacgactc catcgccaac cacgtgtcgc gcgagacata
     4621 cacctacatc accgccgagg ccgcgcgcct cgggttccct gtcgcatccc ttgaccggca
     4681 gagaccgtgg ttcctcgcaa tgacgatgtc ctccatagag ctcatgaagg ccggttacaa
     4741 tccgcattac ggcatcgaca agtacttcct tacaagggcg gccggcagga aaaagctcgt
     4801 ggagctggaa accatcgact accagatcga ccttctggca aggctccccg acaaggagca
     4861 ggagtctttt ctcctctatg cgctgaaaga cctcagctcc ctcgtgcagc aggtcgacag
     4921 actgatggcc gcctggacaa cgggtgacag cgacctgatc gccgagctcc tcgagaagtc
     4981 ggtaggtgaa gacgagagcc tgaaggtgat ccaccggaag atcctgacgg aacgcaacag
     5041 gaacatggcg aagaagatcg ccctgcacct tggatcctcg ggatgcgtgt tcgtggtcgt
     5101 gggagcggca cacctcgtgg gcgacaaagg gatagtggag cttctgagaa aagaggggtt
     5161 cacggtggaa caattataat aatgaaggag cgtcatacat gacacggatc gttttgctgc
     5221 ggcatgggga aagccagtgg aacctggaga acaggttcac gggctggacg gatatcgacc
//
LOCUS       DAOPOA010000061         5251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17185_length_5251_cov_3.670708, whole genome shotgun sequence.
ACCESSION   DAOPOA010000061 DAOPOA010000000
VERSION     DAOPOA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17185_length_5251_cov_3.670708"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKJ17_02195"
     CDS             <1..397
                     /locus_tag="PKJ17_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74807.1"
                     /translation="HGVTVLKGGSHDALAYCDAAIVASGSATLEAALLGAPTAIIYRV
                     SRLSYAIARLIVHVDFIGLPNIVAGKEVFPEFIQRLEPETIARKVISMIDKGRQGVAG
                     ELGSITDKLKKQDSYRLAADAILHFLERT"
     gene            394..2148
                     /gene="msbA"
                     /locus_tag="PKJ17_02200"
     CDS             394..2148
                     /gene="msbA"
                     /locus_tag="PKJ17_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HOD74808.1"
                     /translation="MNLYLRLLRFAYPYKTKLILAMIFMSLCSGAQSFSAYLVKPVLD
                     SIFFEKDLTKLYIMPAAVIVLFLIKGSFEYVQGYFMGYVGQRVVTDIRNLVFYNMQKQ
                     PLSFFDKTPTGSNISRIVNDVSLVQSTVSDSVAAVLKDAFTIIGLVIVAFRMEWKLTL
                     IAFLILPFSIYPIVAFGKKLRKIGLNIQQYLARLTSFLHETITGQRIVKAFSMEGYEN
                     KRFTAENESLFRISMKRTKIRALSHPVMEVIGGVAVGVVIWYGGREVILGNSTPGTFF
                     AFMASLIMLYEPIKKINKENHNVQQGLAASQRVFEIIDRQPEVMEKPDAAQLEKVEGV
                     IDFENVSFRYDEKMVLNNINLRIEKNEVIAVVGKSGVGKTTLVNLIPRFYDVTEGLVR
                     VDGADIRDVTLDSLRRNIALVTQDVILFNDTIRSNIAYGVEPDEERLFEATRMAFAHD
                     FILKLPKGYDTVVGEKGVRLSGGQKQRIAIARALYKNAPILILDEATSSLDAQSEIEV
                     QKALENLMKGRTTIIIAHRLSTIINAHRIIVIDRGMVVQEGTHGSLIEQEGPYRKLYE
                     IQFREGARKKVIPLGKKR"
     gene            2148..2705
                     /locus_tag="PKJ17_02205"
     CDS             2148..2705
                     /locus_tag="PKJ17_02205"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HOD74809.1"
                     /translation="MKKKIKSFLMLHVMPPMVFAFLTALRLTLRVEHVNRPPDEAWGN
                     VRNYIVCFWHGRLLMMPFAKKGGGGKVLISRHRDGEFIARIVNYFGIGTVRGSYRKGS
                     VSSLREILHDLKDNIDIAITPDGPKGPRFTVKKGIVELARLSGKALLPVSYGARKKKP
                     FIPGTASYSPTLSPGSSFSGETLSL"
     gene            2797..4059
                     /locus_tag="PKJ17_02210"
     CDS             2797..4059
                     /locus_tag="PKJ17_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOD74810.1"
                     /translation="MWKVIYNLLTLCALPVFVVIGLANAKMRKNFRARLLPRVKDRPD
                     TGACMIHGASIGEAIIAEGVADYLAKNGGPAHFLFTANTSYAQEMLQRKEGARDGRKV
                     ASLPFDLRFSVRRFLSHHNPSAIIIVETEIWPNLIWEAGRRRIPVVIVNGRISDSTLA
                     SYRRLSVFMRSALAGVDAVIAQSAEHRQRFISIGMAPERVFATGNIKYYRPVADSIPG
                     REERDRIIAIGSVKERELDEIYRAVKLLKSDLPDHRIFIAPRELHLAATIEEELSKTF
                     RTARYSRIKEGTDEDRADIVVVDTVGDLMGIYARSVAAFVGGSLAPYGGQNMLEPLFV
                     GTPVLFGPHTENFHDIAASILERKAGFLVETADDIHRAVMRLLTDNDLYESTRQAGFA
                     IVAGQKDVMDETARIIRGLIEPRSGREG"
     gene            4062..4823
                     /gene="mazG"
                     /locus_tag="PKJ17_02215"
     CDS             4062..4823
                     /gene="mazG"
                     /locus_tag="PKJ17_02215"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOD74811.1"
                     /translation="MESFDKLVWLMERLRSEEGCPWDRKQKVSAFKTFMLEEVYEIIE
                     AIEKDDMKDLKEELGDLLFHIVFIAQICRERDAFDINDVIENVYGKMYHRHPHVFRKE
                     VLDVPIEMKWEDLKREEKRDTGYSLLANIPPSMPALLRAYVMTKRAAKVGFDWQKIED
                     VYDKMYEEIAELKAAEAAGDQELVREEVGDLIFTIVNIARFLSVDPEDALRATSDKFT
                     RRFSYIENNTDIRNSDADTMDKLWNDSKSLEDKGK"
     gene            complement(4832..5035)
                     /locus_tag="PKJ17_02220"
     CDS             complement(4832..5035)
                     /locus_tag="PKJ17_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOD74812.1"
                     /translation="MPLGKVKWFNDSKGYGFIEQDGGEDVFVHFSAIQQDGFKSLKTG
                     EKVEFEIVKGPKGPQAANVMKAE"
BASE COUNT         1212 a         1584 c         1438 g         1017 t
ORIGIN      
        1 tcatggagta acggtcctga aagggggctc gcacgatgcg ctggcctatt gcgacgccgc
       61 catagtcgct tccggcagcg cgaccctgga ggcggcccta ctcggcgccc ccacagccat
      121 catctaccgc gtatcccggc tctcctacgc catcgcgcgc ctcatcgtcc acgtcgactt
      181 catcggcctt cccaacatcg tcgcaggcaa ggaggtcttc ccggagttca ttcagcgcct
      241 cgagcccgaa accattgcca ggaaggtcat ttctatgata gataaaggga gacagggcgt
      301 cgcgggcgaa ttgggatcga tcaccgacaa acttaagaaa caggattcct accggttggc
      361 agcggacgcc atactacact ttctcgagag aacatgaacc tctacctgag acttcttaga
      421 ttcgcctatc cttacaagac aaagctcatc ctcgccatga tcttcatgtc cctgtgctcc
      481 ggcgcgcaga gcttcagcgc ttacctcgtc aaaccggtcc ttgactccat attctttgag
      541 aaggacctga cgaagctcta catcatgccc gcggcggtca tcgttctctt cctgatcaag
      601 ggctccttcg agtacgttca gggttacttc atgggctatg tcgggcagag ggtggtcaca
      661 gacatcagga accttgtctt ctacaacatg cagaagcaac ccctctcctt cttcgacaag
      721 acacccacgg gttcgaacat ctcccggata gtgaacgatg tctccctcgt ccagagcacc
      781 gtctccgatt ccgtcgcggc agtcctcaag gacgccttca ccatcatcgg actggtcatt
      841 gtggccttcc gcatggaatg gaaactgacg ctcatcgcct tcctgatcct gcccttttcc
      901 atctatccaa ttgtcgcctt cggcaagaag ctcaggaaga tagggctcaa tatccagcaa
      961 tacctggcgc gcctcaccag cttcctccat gagaccatca ccggacagag gatcgtcaag
     1021 gccttctcca tggagggata cgagaacaag agattcaccg cggagaacga atcgctcttc
     1081 aggatctcca tgaaacgcac caagatccgg gcgttgtccc accccgtgat ggaggtcatc
     1141 ggcggggtcg ctgtcggtgt cgtcatctgg tatgggggcc gggaggtcat cctgggaaac
     1201 tccacccccg gtaccttctt cgccttcatg gcatccctta tcatgctcta cgagcccatc
     1261 aagaagatca acaaggagaa ccacaacgtc cagcaggggc tcgccgccag tcagcgcgtc
     1321 ttcgagatca tcgacaggca gcccgaggtc atggagaagc cggatgccgc ccaactcgag
     1381 aaggtcgagg gtgtcataga cttcgagaac gtctccttcc gatacgatga aaagatggtc
     1441 ctcaacaaca taaacctcag gatcgagaag aacgaggtga tcgccgtcgt cgggaagagc
     1501 ggtgtgggga aaaccactct cgtcaatctg atcccgaggt tctacgacgt caccgagggt
     1561 ctcgtcaggg tcgacggcgc cgatatccgt gacgtgaccc ttgattccct cagaaggaac
     1621 atcgcccttg tcacgcagga cgtcatcctc ttcaacgata cgatacggag caacatcgcc
     1681 tacggggtcg agccggacga agagaggctt ttcgaggcga cccggatggc cttcgcccac
     1741 gatttcatcc tcaagctccc caagggatac gacaccgtcg tcggcgaaaa aggggtgcgt
     1801 ctttccggcg gccagaagca gcgcatcgcc atagccaggg ccctttacaa gaacgctccc
     1861 attctcatcc tcgatgaggc cacgagctcc ctcgacgccc aatcagagat agaggtccag
     1921 aaggccctcg agaacctgat gaaagggagg acgacgataa tcatcgccca caggctctcc
     1981 acgatcatca acgcccatcg gatcatcgtc atcgacaggg ggatggtggt ccaggaaggc
     2041 acccatgggt cgctcatcga gcaggaaggg ccctacagga agctctacga gatccagttc
     2101 cgcgagggag cccgcaagaa ggtcatcccc ctgggaaaga aacgctgatg aagaagaaga
     2161 tcaaatcctt cctgatgctg cacgtcatgc ctcccatggt attcgcgttc ctgacggcac
     2221 tgaggttgac gctgcgcgtt gaacacgtga acaggccgcc cgacgaggca tgggggaacg
     2281 tccgcaacta catcgtctgt ttctggcatg gcaggctcct catgatgccc ttcgcgaaga
     2341 agggcggcgg gggcaaggtg ctgatcagtc gccaccgcga cggagagttc attgccagga
     2401 tagtgaacta cttcgggatc ggcaccgtcc ggggctcata cagaaagggc agcgtctcct
     2461 cgctcaggga gatcctccac gacctcaagg acaatatcga tatcgccatc acccctgacg
     2521 gcccgaaagg gccccgtttt accgtgaaga aaggcatcgt ggagctcgcg cggctctccg
     2581 gcaaggccct cctccccgtc agttacggtg cgcgtaaaaa aaaacctttc attcctggga
     2641 ccgcttcata ctcccctacc ctttctcccg ggtcgtcttt ctctggggag accctatccc
     2701 tgtagaccgt gacctcagtg ccgaagaaac agagaaaacg cgcctcaagc tcgaggcaac
     2761 gcttgtccaa ctgaccgaaa gagcggaaga agcggcatgt ggaaagtgat ctacaatctg
     2821 ctcaccctct gcgccctgcc cgtcttcgtc gtcatcgggc ttgccaacgc gaagatgagg
     2881 aagaacttcc gcgcgcgcct tctcccgcgg gtgaaagaca ggccggatac gggtgcctgc
     2941 atgatccacg gggcgtcgat aggagaggcc attatcgccg agggcgtggc cgactacctc
     3001 gcgaagaacg gtgggccggc tcattttctc ttcaccgcca acacatcata cgctcaggaa
     3061 atgcttcaaa gaaaagaagg cgccagggac ggcaggaagg tggcctctct acccttcgac
     3121 ctgcgcttct ccgtgcgacg cttcctctcc caccacaatc cctcggccat catcatcgtg
     3181 gaaacggaga tctggcccaa cctgatctgg gaggctggca gacggcgcat cccggtcgtt
     3241 atcgtcaacg ggcgcatatc ggacagcacc ctcgccagtt accgcaggct gtccgtgttc
     3301 atgagatcgg ctctggctgg ggtagacgcc gtcatcgcgc agtccgcgga gcacaggcag
     3361 cgcttcattt ccatcggcat ggccccggag cgggtcttcg ccacgggcaa catcaagtac
     3421 taccgtcccg ttgccgacag catcccgggc agggaagaac gcgacaggat catagccatc
     3481 ggcagcgtga aggaaaggga gctcgacgag atatacaggg cggtcaagct cctcaaatcc
     3541 gacctcccgg atcacaggat attcatagcg cccagggaac tccatctggc cgccacgatc
     3601 gaggaggagc tttcgaagac cttcaggacc gcccgctatt cccgcataaa ggagggcacg
     3661 gatgaggacc gcgccgacat cgtggtcgtc gataccgtgg gcgacctcat ggggatctac
     3721 gcgagaagcg tggccgcctt cgtaggcggt tcccttgctc cctacggcgg tcagaacatg
     3781 ctcgagcccc tcttcgtggg cacacccgtg ctgttcgggc cacacacgga aaacttccac
     3841 gacattgccg cctccatcct tgaaagaaag gcaggtttcc tcgtggaaac cgccgacgac
     3901 atacaccgcg cggtgatgcg gctcctcacc gataacgatc tctacgagtc cacacggcag
     3961 gccggtttcg ccatcgtcgc cggtcagaag gacgttatgg acgagacggc gcggatcata
     4021 aggggcctca tcgagccccg gagcgggaga gagggctgag aatggaatcc tttgacaaac
     4081 tcgtatggct gatggaaagg ctcaggagcg aggaaggatg cccctgggac cgcaaacaga
     4141 aggtttccgc cttcaagacc ttcatgctcg aagaggtcta cgagatcatc gaggccatag
     4201 aaaaggacga catgaaggat ctcaaggaag agctcgggga cctcctcttc cacatcgtct
     4261 tcatcgcgca gatatgcagg gagcgcgatg ccttcgacat caatgacgtt atcgagaacg
     4321 tctacggcaa gatgtaccac cgtcatcccc atgtcttccg gaaagaggtg ctggacgtcc
     4381 ccatcgagat gaagtgggag gacctgaaga gggaagagaa gagggatacc gggtactccc
     4441 tcctcgcgaa catccccccg tccatgcccg ccctcctgag ggcatacgtc atgacgaaga
     4501 gggccgcgaa ggtcgggttc gactggcaga agatcgagga cgtgtacgac aagatgtacg
     4561 aggagatcgc cgagctgaag gcggcagagg cggcaggcga tcaggaactg gtcagggagg
     4621 aggtgggcga cctaattttc accatcgtca acatcgcccg gttcctgtcc gtggaccccg
     4681 aagacgcgct acgggcaacc tcggacaagt tcactcgaag gttcagctac attgagaaca
     4741 atacggatat ccggaattct gatgccgaca cgatggacaa actctggaat gacagtaaat
     4801 ctttggagga caagggcaaa tagcccctga ctcactcggc cttcattaca tttgccgcct
     4861 gcggaccctt cggccccttc acgatctcga attcgacctt ctcaccggtc ttgagcgatt
     4921 tgaaaccatc ctgctggatc gcggagaagt gcacgaaaac atcctccccg ccgtcctgtt
     4981 cgatgaaacc gtaccccttg gaatcgttga accatttcac cttacctagt ggcatactgc
     5041 atgactcctt tctttgggaa tgttaagcat ttgtggcttt taagcaatat tattcctaat
     5101 aaaaatagct gtcaagcgaa atctgtcggt atcccttgat attatacggg aaatcgcatt
     5161 ttggagggcg taagcctatc tgaccgcgaa ccccaccgaa tcgacgatgc tgccatcgcg
     5221 gtcgacgatg gaaagggtat aattgccccg c
//
LOCUS       DAOPOA010000062         5246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17201_length_5246_cov_3.709690, whole genome shotgun sequence.
ACCESSION   DAOPOA010000062 DAOPOA010000000
VERSION     DAOPOA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17201_length_5246_cov_3.709690"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PKJ17_02225"
     CDS             <1..1079
                     /locus_tag="PKJ17_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOD74813.1"
                     /translation="LKNTTFFKDTDFKIVKTDDPRKVNIDLGIEEKPTGTFAIGAGYS
                     TAEKVIVSGSVSEENFLGTGRKVVLEAALSSYTNEFRLSYLEPYIFDKNLSVGTSLYN
                     FKRYMDTYDYKRMGGNLSLIKPLTDDVKGTVMYRLENIDVTNVTNDASSYIKQQSGSS
                     LTSSMTFSLNKNTIDDVMNPTKGINSGISTEIAGGPLGADNKFWSITGFYGRYFPITF
                     WDSSFFVKGTAGSIRGYGGDEVPITEKYYVGGLNSIRGFRYGEAGPMDENDEPIGAKN
                     QMYFNFEWIFPIYKPAGVKGVLFTDTGAGFDNFDSLRMRSSAGFGIRWFSPMGPIRLE
                     MGFNLNPKENERKSLFDFAIGTQY"
     gene            1092..1625
                     /locus_tag="PKJ17_02230"
     CDS             1092..1625
                     /locus_tag="PKJ17_02230"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOD74814.1"
                     /translation="MKRFITVFFVAAVLLTPLAAMSQSVSVAYIDLQKVMLESDQGKQ
                     IRAILTQDAERLKKNLDGKQAELQRLKDAFEKQGATITPEARADKEKQYQAKLKDYQR
                     LYNDYQSELQQKDMEYTQKVLKEVEEVVRALGEKEKYSLILEKSQAGILYATPAIDIT
                     GKVITLFNQAAKNKPLK"
     gene            1625..2665
                     /gene="lpxD"
                     /locus_tag="PKJ17_02235"
     CDS             1625..2665
                     /gene="lpxD"
                     /locus_tag="PKJ17_02235"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOD74815.1"
                     /translation="MIRLRDIAAAVKGDLIGDGDIPIEGIAGIQSAREGQITFLSHAS
                     YVKHVAGCRASALIIGQKTPRDDVRGMNIVVVENPDLAYIEVVGMFRKEPSPPAGIDP
                     RAAVSPEAEVAASASVGPFACIERQARIGEKVTVYPFCYIGPNVTIGDGSVIYPHVTI
                     YRDTTIGRNVIIHANTVIAADGFSYTWDGKRHAKIPQIGSVIIEDDVEIGANTDIDRA
                     ALDATVVGRGTKIDNLVQVAHNVTIGQNSIIVSQVGIAGSAHIGNNVVLAGQTGVKDH
                     VSVGDQVMAGGRTGITKDVPAGSLIWGTPHMPHKEYMRLQMYIKKLPALFEKIRELEK
                     RLSPEEDDDRHK"
     gene            2649..3077
                     /gene="fabZ"
                     /locus_tag="PKJ17_02240"
     CDS             2649..3077
                     /gene="fabZ"
                     /locus_tag="PKJ17_02240"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOD74816.1"
                     /translation="MIDINEILSLMPHRYPFLLVDRVIEFEETKRIVAIKNVTINEPF
                     FQGHFPGRPIMPGVLILEAMAQAGGVLAFKSFPGMKGTVFFTGIDGARFRKPVIPGDQ
                     LRFVVDVVKHRREIWVFEAKAYVGDDVVAEARIMAMLKQE"
     gene            3089..3856
                     /gene="lpxA"
                     /locus_tag="PKJ17_02245"
     CDS             3089..3856
                     /gene="lpxA"
                     /locus_tag="PKJ17_02245"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOD74817.1"
                     /translation="MIHPTAIVDSSAEIAGDVEIGPYCIVEGGVRIGAGSRLESHVVV
                     QGNTVIGEGCTVSPFASIGGPPQDIGYKYEPTRVVIGRNNTIKEYVTINRGTMKGGGV
                     TEVGENNFIMAYAHIAHDCKVGNNIIMANGATLAGHVEVSDFVIFSGLCAVHQFCRVG
                     KYAFISGLTGIPKDVPPFVIAAGDRAKLYGLNLVGLERRNFSKDEIMKLKKAYRILFR
                     SALPLTTSLKIIREELDGDNIGYLIEFIESSTRGICR"
     gene            3847..4782
                     /locus_tag="PKJ17_02250"
     CDS             3847..4782
                     /locus_tag="PKJ17_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOD74818.1"
                     /translation="MPVNVALIGLGHMGRIHLGKLCSFEDVRVCGVVDVDDSLAGEYA
                     GKYTVPSFSHYSDILPDAACAVIATPTQSHYAIAKACLERGVHVFLEKPITVLPEEAR
                     ELVDMAAARGLVLQVGHLERLNPALRQALPLVRRPRFIEARRISPFTGRSTDVDVVLD
                     LMIHDIDLVLSLVGEEVADVRAQGVPFFTSLTDFASARIDFVGGCVANITASRISTFR
                     ERSLSIYEEDRYLSVDLMQGKLTSIIRKEGEGSDTTEYTAAQMDPVRDELLEFVRAVG
                     GNSTPSVSGTDGLRALELASRVQDCIAGGQGTGHR"
     gene            4808..>5246
                     /locus_tag="PKJ17_02255"
     CDS             4808..>5246
                     /locus_tag="PKJ17_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74819.1"
                     /translation="MSGETHARHLVESLSRSADLRITAMGSDRLAGAGVRVVRDYRDI
                     SVTGLSEVLSHAGNIRRAFDTVKTHIRAERPDLVVLVDFPGFNMRIASFARSLRIPVV
                     YFIPPQVWAWKRGRLKKIRRYVDMVICILPFEENLYANAGVPAV"
BASE COUNT         1218 a         1578 c         1508 g          942 t
ORIGIN      
        1 agctgaagaa caccacattc tttaaggaca ccgatttcaa gatcgtcaag accgatgacc
       61 ccaggaaggt caacatcgac ctcggcatcg aggaaaaacc cacgggcacc ttcgctatcg
      121 gggcaggcta cagcacggcg gagaaggtga tcgtttccgg gtccgtctcg gaagagaact
      181 tccttggcac gggccggaag gtggtcctcg aggccgcgct cagcagttac accaacgagt
      241 tccggctcag ctacctggaa ccctacatct tcgacaagaa cctctccgtc gggaccagtc
      301 tctacaattt caagcggtac atggacacgt acgactacaa gcggatgggc ggcaacctgt
      361 ccctcatcaa gcccctcacc gatgacgtca agggaacggt catgtaccgt ctcgagaaca
      421 tcgacgtcac gaacgttacg aacgacgcca gcagctacat caagcagcag tccggttcga
      481 gcctgacgag cagtatgacc ttcagtctca acaagaacac catcgatgac gtcatgaacc
      541 ccacgaaggg cataaacagc ggcatatcaa cggaaatagc cggcggtccc ctgggagccg
      601 acaacaagtt ctggtctatc accggttttt acggcaggta cttccccatc accttctggg
      661 acagctcctt cttcgtcaaa ggcaccgcgg gatcgatcag gggctatggc ggggacgagg
      721 tgccgatcac ggagaaatac tatgtcggcg gtctgaactc catccgcggt ttcaggtacg
      781 gcgaagccgg gcccatggac gagaacgacg agcccatcgg cgcgaagaac cagatgtact
      841 tcaacttcga atggatattc cccatctaca agccggcagg ggtgaagggg gtccttttca
      901 ccgacaccgg tgccgggttc gataacttcg acagcctgcg gatgcggtca tcggccggat
      961 tcggcatacg ctggttctct cccatgggtc ctatccgcct ggaaatgggc tttaacctca
     1021 accccaaaga aaacgaaagg aagagtcttt tcgattttgc catcggtaca caatattagt
     1081 caggaggttt tatgaaacgc tttatcacgg tcttttttgt tgcagccgta cttttgacac
     1141 cacttgcggc gatgagccag tcggtcagcg tcgcgtacat agacctgcag aaagtgatgc
     1201 ttgaatcgga ccagggcaaa cagatacggg ccattctcac gcaagatgcc gagagactga
     1261 aaaagaacct cgacggaaag caggccgagc tccagaggct caaggacgcc ttcgagaaac
     1321 agggcgccac catcacgccc gaagccaggg ccgacaagga aaaacagtac caggcaaagc
     1381 tcaaggatta ccagaggctc tacaacgact accagtccga gctccagcag aaggacatgg
     1441 aatacacgca gaaggtcctc aaggaggtcg aagaggtggt gcgcgccctt ggtgagaagg
     1501 agaagtattc cctcatactt gaaaagagcc aggccggcat cctgtacgcg acccccgcca
     1561 tagacatcac gggcaaagtg atcacactct tcaaccaagc ggccaagaat aaacctctca
     1621 agtaatgatc agactgaggg atatcgctgc cgcggtcaag ggagatctga ttggggacgg
     1681 ggatatcccg atcgagggca ttgccggcat tcagagcgcc cgggaaggac agatcacctt
     1741 cctctcccac gcgagctatg tcaagcatgt cgccgggtgc agggcctccg cccttatcat
     1801 agggcagaag actccccgcg atgacgtccg gggaatgaac atcgtcgttg tggagaaccc
     1861 cgacctcgcg tatatagagg tcgtgggcat gttccggaag gaaccctccc cgcctgccgg
     1921 gatagacccg cgcgccgcgg taagccccga ggccgaggtc gccgccagtg cctccgtcgg
     1981 ccccttcgcc tgcatagagc ggcaggcaag gatcggggag aaggtgacgg tctatccctt
     2041 ctgctatatc ggtcccaacg tcacaatcgg cgatggcagc gtcatctatc cccacgtcac
     2101 catctaccgg gacacgacga tcgggagaaa tgtcatcatc cacgcgaaca ccgtcatagc
     2161 cgccgacggc ttcagctaca cctgggacgg gaaaaggcac gccaagatcc cccagatcgg
     2221 ctccgtcatc atagaggacg atgtggagat cggggccaat accgatattg accgcgcagc
     2281 gcttgacgcc acggtggtgg gcagggggac gaagatcgac aacctcgttc aggtcgccca
     2341 caatgttact atcggacaga actccatcat cgtctcccag gtcggcatag cgggcagcgc
     2401 gcacataggg aacaacgtcg tcctcgcggg ccagacgggg gtcaaggacc acgtcagcgt
     2461 gggcgatcag gtcatggcgg ggggacggac ggggatcacg aaagacgttc ccgccgggtc
     2521 tctcatatgg gggaccccgc acatgcccca caaggaatac atgagactcc agatgtacat
     2581 aaagaagctc cccgcccttt ttgaaaagat cagggaactg gagaagagac tctcaccgga
     2641 ggaagacgat gatcgacata aatgagatac tgagcctgat gccccacaga tacccctttc
     2701 tcctcgtcga ccgtgtcatc gaattcgaag agacaaagag gatagtcgcc ataaagaacg
     2761 tgaccatcaa cgagcctttc ttccagggtc acttcccggg aaggcccatc atgcccggcg
     2821 ttctcatcct tgaagcgatg gcgcaggcag gcggcgtcct tgccttcaag tccttccccg
     2881 gcatgaaggg taccgtcttc tttaccggca tcgacggggc acggttccgc aaaccggtga
     2941 tccccggcga ccagctcagg ttcgttgtcg acgtcgtgaa acacagacgg gagatctggg
     3001 tattcgaggc aaaggcctac gtcggagacg acgtcgtcgc cgaggcgcgt attatggcca
     3061 tgttaaaaca ggagtgaggg agacttacgt gatacatcca accgccatcg ttgacagcag
     3121 cgccgagatc gcaggagatg tcgagatcgg tccctattgc atagttgaag ggggcgtgcg
     3181 catcggcgcg ggcagccgcc tcgagagcca tgtcgtcgtt cagggaaaca ccgtcatagg
     3241 cgaaggctgc accgtgagcc ctttcgcctc catcggagga cctccccagg acatcggcta
     3301 caagtacgaa cccacccggg ttgtcatagg ccgcaacaac accataaagg aatacgtcac
     3361 catcaaccgg ggaaccatga aagggggcgg cgtcaccgag gtgggggaga acaatttcat
     3421 catggcatac gctcacatcg cccacgactg caaggtggga aacaatataa tcatggcgaa
     3481 cggggcaacc ctggcgggcc atgtggaggt ttccgatttt gtcatcttca gcggcctctg
     3541 cgccgtccac cagttctgca gggtcggcaa gtacgccttc atcagcggtc tgacggggat
     3601 ccccaaggat gtgcctccct tcgtcatcgc cgccggtgac agggcaaaac tctacggcct
     3661 caacctcgtg ggcctcgaaa ggcgcaattt ctccaaggat gagataatga agctgaagaa
     3721 ggcataccgc atcctcttca ggtccgctct ccccctcacg acatccctca agatcatcag
     3781 ggaggaactg gacggggaca atatcgggta tctcatcgag ttcatagaat cgtcaacgag
     3841 ggggatatgc cggtaaacgt cgccctcatc ggtctcggcc acatgggccg catccatctc
     3901 ggcaagctct gctcctttga ggacgtccgc gtctgcgggg ttgtggatgt cgatgacagt
     3961 ctcgccggtg aatatgccgg gaaatacacg gtcccctcct tctcccatta ttccgatatc
     4021 ctgcccgacg cggcctgcgc cgtcatcgcc acaccgactc agtcccacta cgccatcgcg
     4081 aaggcctgtc tggaacgggg cgtccatgtc ttcctggaaa aacccatcac cgtgctccct
     4141 gaagaggcac gggagctcgt cgacatggcg gccgcgcgag ggctcgtcct ccaggtgggc
     4201 catctcgaaa ggctgaaccc cgcgctcaga caggctctcc ccctcgtccg gaggccgcgg
     4261 ttcatcgagg cgcgcaggat cagtcccttc acgggcaggt ccaccgacgt ggacgtcgtc
     4321 ctcgacctca tgatccacga catcgatctt gtcctgtccc tcgtcggtga agaggtcgcg
     4381 gatgtcaggg cgcagggcgt ccccttcttc acgagcctga cggacttcgc cagcgcccgg
     4441 atcgacttcg tcggcgggtg cgtggcgaac atcacggcga gccgtatctc cacgttccgc
     4501 gagcgctccc tctccatcta cgaggaagac aggtatctct ccgtcgacct gatgcaggga
     4561 aagctcacca gcatcataag gaaggaaggg gaagggtcgg acacaacgga gtacaccgcc
     4621 gcgcagatgg accccgtcag ggacgaactc ctcgagttcg tccgggccgt gggaggcaac
     4681 tcaaccccca gcgtcagcgg aaccgacggt ctcagggcac tcgagcttgc aagcagggtc
     4741 caggactgca tagcgggcgg acagggtaca ggccatcgat aaaaagatat tcatatcgac
     4801 gggtgagttg tccggggaga cccatgcccg ccatctcgtc gaatccctca gcaggtcagc
     4861 agatctgcgt ataacagcca tgggttccga caggctcgcc ggcgccggcg tcagggttgt
     4921 ccgggattac cgggacatct ccgtgacggg cctcagcgag gtgctctccc acgcggggaa
     4981 catcaggagg gccttcgaca ccgtcaagac ccatatcagg gcggagcgac cggacctcgt
     5041 cgtgctcgtc gacttcccgg gattcaacat gaggatagcc tccttcgccc gctccctccg
     5101 aattcccgtg gtatacttca tcccgccgca ggtatgggcc tggaagcggg ggcgcctcaa
     5161 gaagatcaga agatacgtcg acatggtgat ctgcatcctt cccttcgagg aaaacctgta
     5221 cgcgaacgcg ggggtcccgg cagtct
//
LOCUS       DAOPOA010000063         5241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17231_length_5241_cov_2.972811, whole genome shotgun sequence.
ACCESSION   DAOPOA010000063 DAOPOA010000000
VERSION     DAOPOA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17231_length_5241_cov_2.972811"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1030
                     /locus_tag="PKJ17_02260"
     CDS             206..1030
                     /locus_tag="PKJ17_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HOD74820.1"
                     /translation="MVPYQGRGDSETVTNIISAAEITGAADLMNAYMSGGFLADFVTV
                     MASSMALSGGNALIIALAALTLPPARRLQGVVLGTVAAILARVLIACLPAALLNVPCL
                     KLCCGLLILTIGLKLILDGHSPQEKKEVSGGSMKAFLMIILADLAMSPDNILAMAGAS
                     TGNPALAFLGLSFSIPMVAFGGGVMSRLMGHYPPAIYAGAAILGKVAADTVLADPLTL
                     RLLHAPGPALRYGAEMLVAVGVVAAGIFLVRLREMRHPPVPAPHSGPFHVTGWDIR"
     gene            complement(1462..2028)
                     /locus_tag="PKJ17_02265"
     CDS             complement(1462..2028)
                     /locus_tag="PKJ17_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOD74821.1"
                     /translation="MRTFAAAVVVILAFAGLVVYAPSYLFYADPPRKSDAVVLFLGNE
                     YKERRAEAVKLIGEGYAAYLVIPAYGRMVEAGKHGEASRNVVPSRPSHYPVVYEDTHV
                     EIIEAKKLMDRKGLTSAIFVSSPHHMRRIKLIADRVFIGGAYVTTFVPTRFEKPGQGF
                     WFLRESDIRKVTSEYGKILWFFLYRWMK"
     gene            complement(2039..3022)
                     /gene="galE"
                     /locus_tag="PKJ17_02270"
     CDS             complement(2039..3022)
                     /gene="galE"
                     /locus_tag="PKJ17_02270"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOD74822.1"
                     /translation="MKIFVTGGAGYIGSHVVKLLGEQGHNVLVYDNLSTGHEWAVLAG
                     RLVKGDLSDRQALKDTLREYSPDAVMHFAASIQVEESVREPLMYYRNNVINSINLLEA
                     MTELKIGYFIYSSTAATYGAPTVSPVDEDTPLTPINPYGMTKVMVESVLRDLAAKGDL
                     RYIALRYFNVAGADRQARIGQAYREATHLITRALRTAQGQQEKLFVFGTDYPTTDGTC
                     VRDYIHVEDLASAHLLALMYLRERGASDVMNCGYGHGFSVLDVVRAAKAVTGVDFPVE
                     LAGRRPGDPPELVADSSKIRRLTGWSPLYDDLGFIIETAWKWEKKRSATKD"
     gene            3448..3930
                     /locus_tag="PKJ17_02275"
     CDS             3448..3930
                     /locus_tag="PKJ17_02275"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOD74823.1"
                     /translation="MKKDVTICFRTNEELKQTLEKIAEVERRSLSSIIENMLHSQVKD
                     KVELKGSKKERRRYARKEVSLPAFVFKRDAKESTLQTGIVIDVSLGGLKISIPHDYDA
                     VSEGEFDTIFTIPNEKTPVKMRCTIKRIAEGEEATKELGADFIDGDFLSYQKLHKYLS
                     "
     gene            complement(4012..>5241)
                     /locus_tag="PKJ17_02280"
     CDS             complement(4012..>5241)
                     /locus_tag="PKJ17_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD74824.1"
                     /translation="IAAILRRNGFHVDLIDCMSVSEEKRKPDGRAPFRKHKVPKPDAL
                     KGVRKTLRRYGMPPEELSALLEGMEPPDIILVTSIMTYWYVGAMEVVDLARRVHPRSK
                     IVVGGIYPTLCREQASLRFAAADLVVSSGELGDFYGYVEAATGRRPGFTPHMDNLDIL
                     PYPAHDLCRDIPFVPLLTSLGCAFRCTYCATSYMYPAMARRGVDSVVDEILHWHDRGV
                     RRFVVYDDSFLFDSSRHAKPLLERIAGLPFPVDIYNPNALNAAFIDDEVANLLVSSGF
                     REVRIGLESVDPGRQAQTGGKVTGALFERAVGALRRAGYGRGQIRAYILAGLPGQPWQ
                     EVRRAIDYLKALDVTPSIAEYTPIPHTPLFDKHKDSARFPVADDAIFQNNALFPYAWE
                     GFTEEDLDYLKKYAKKG"
BASE COUNT         1067 a         1533 c         1603 g         1038 t
ORIGIN      
        1 tacgcagcgt cgcggaactg accaagtacg ccatcaggga aggcataacc tccctgtaga
       61 gaccccgcga aggaagacct tgagccctct tcgaccggga taagggccga aagacgcggt
      121 ggtcgcgcca gcgtgcgtct cagggaaaat acccccgaaa atcaggaaaa ccccgatacc
      181 ttcacccctt ctgcaaggat atactatggt tccgtatcag gggcgaggag acagcgagac
      241 cgtgaccaac attataagcg cagcggagat aaccggagcc gcggacctca tgaacgctta
      301 catgagcgga gggttcctcg cagacttcgt gacggtaatg gcgtcgagca tggcgctctc
      361 cggggggaac gccctgatca ttgcccttgc cgccctgacg cttcctcctg cccggcggct
      421 tcagggggtt gtcctcggaa ccgtcgccgc catcctggcg agggtcctca tcgcctgcct
      481 ccccgcggcg cttctcaatg tcccctgcct gaagctttgc tgcggacttc tcattcttac
      541 catcgggctg aagctcatcc tcgacggtca ttcaccgcag gagaaaaagg aggtttcggg
      601 cgggtccatg aaggccttcc tcatgatcat cctcgcggac ctggcgatga gtcccgacaa
      661 catcctcgcc atggcgggcg ccagcacggg caacccggcc ctggcctttc tcgggctgag
      721 cttcagcata cccatggtgg cattcggcgg aggtgtgatg tcgcggctca tgggacacta
      781 tccccccgcg atctatgccg gcgcggccat tcttggcaag gtcgcggcgg acactgtcct
      841 tgccgatcca ttgaccttga gactgttgca tgctccgggt cccgcactgc ggtatggcgc
      901 cgaaatgctg gtggccgtcg gcgtcgttgc cgcggggatc ttccttgtca ggctgcggga
      961 gatgaggcat ccccccgtcc ctgcccctca ttcggggcct ttccacgtga cgggttggga
     1021 catacgatga cgtcgctcca cctcgggagg gtgcgggcga tgggagaaaa aaggaggacc
     1081 gttcagcgcg cggactagcg cccacaggac cgatcgggga aggacgctgc agggagaagg
     1141 gggtaggaag cttcaagacg ccggaaagac ggtatttggg tccagcgtcg cagcccctgc
     1201 cggtcctgtg agaaagggat aagatgtaaa aggcccatcg cggccgcgca gcaaacgttc
     1261 atcagtaccc cgacggaggt cgggatccaa tcgcccttgc cctgcgttac aatcaggatc
     1321 ccggcctcca ccgcaaattc cccactggta ctagtccaga taggaccgat aggaagtggt
     1381 aagacccacg ggacgtataa gaccaatagg acggatagga cctatcgatg gcgtttttgg
     1441 gttgggacgc cggaccgtgt tctacttcat ccacctgtag agaaagaacc acaggatctt
     1501 gccgtattcg ctggtgacct tccttatatc cgactcgcgc aggaaccaga aaccctgtcc
     1561 cggcttctcg aaacgggtcg gaacgaaggt ggtgacatag gctccgccaa tgaagaccct
     1621 gtcggcgatg agctttatcc gcctcatgtg gtggggggag ctgacgaata tggcggaggt
     1681 cagacccttc cggtccatca gcttctttgc ctcgatgatc tcaacatggg tgtcctcgta
     1741 gactacgggg tagtgtgagg gccgcgaggg aacgacgttg cgcgacgcct cgccgtgttt
     1801 gcccgcctcg accatcctgc cgtaagcggg aataacgaga taggcggcgt atccttcccc
     1861 tatgagcttc accgcctcgg ccctgcgctc tttgtactcg ttgccgagga acagaacgac
     1921 ggcatccgac ttccggggag gatcggcata gaagaggtag ctcggtgcgt agaccacaag
     1981 gccggcaaaa gcgagaatga cgacaacggc cgcggcaaag gtcctcatgc gtgatccttc
     2041 aatccttcgt cgccgacctc ttcttttccc atttccatgc tgtttcgatg atgaagccaa
     2101 gatcgtcgta gagtggagac caccccgtca gcctcctgat cttcgagctg tcggcaacga
     2161 gctcgggtgg gtcgccgggc ctgcgccccg ccagttccac ggggaagtcc acccccgtca
     2221 ccgcttttgc cgcgcggacc acgtccagaa cggagaatcc atggccatac ccgcagttca
     2281 tcacatcaga ggcgcctctt tccctcaggt acatgagggc cagaaggtgc gcggacgcca
     2341 ggtcttccac atggatgtag tccctcacgc aggtcccgtc tgtcgtgggg tagtcggtgc
     2401 cgaagacaaa gagcttctcc tgttggccct gtgccgtcct cagggcccgc gtgataagat
     2461 gcgtcgcctc gcgataggcc tgacctatcc gcgcctgacg gtcggcgccg gcaacattga
     2521 aataccgcag cgcaatataa cggaggtccc ccttcgcggc caggtcccga agcaccgact
     2581 cgaccatgac cttcgtcatg ccatagggat tgatgggagt aaggggcgta tcctcatcga
     2641 cggggctcac cgtcggcgcc ccgtaggtgg cggccgtcga cgaatagatg aaatacccga
     2701 tcttgagctc ggtcatcgcc tcgaggaggt tgatgctgtt gatgacgttg ttccggtagt
     2761 acatgagagg ttcccggacg gattcttcca cctggatgga cgcggcgaaa tgcatgacag
     2821 cgtcgggaga gtattccctc agggtgtctt tgagagcttg cctgtcggaa aggtcgccct
     2881 tgacgaggcg ccccgccagc acggcccatt catggcccgt ggaaagattg tcgtagacga
     2941 gaacgttgtg tccctgttct ccgaggagct ttacaacgtg gctgccgata tagccggcac
     3001 cacccgtgac aaagatcttc attcgtccct cctttgcggg acctgccgtc atctccccga
     3061 ctgcgccggc gcaacgaaag agcgcggcgg catgggagaa acccgatgga tcttcccccc
     3121 ttcccgggtt gcccggggcc ggaatgatga aaaacgccac agcgggcgtg tccccgtcct
     3181 gatatctgcc cggttccgat tgagcactat tgtaccacaa ttgctcacga taagtaattg
     3241 tttcccggtc cgggcccggc gggaagaagg ggaagaatgc cacgtgccgg acgcccgggg
     3301 gatgccgcct ttcccgcatt atataaggcg ttaaggaccc ttttgcggtc aagttcagct
     3361 tttcaatagt tccatgttat gttatactga tgtatcgcaa aatgctgcga ggtttgcgcc
     3421 agatagcgca gtgtaagggg gacggcaatg aagaaagacg taacgatatg ttttcggacc
     3481 aacgaggagt tgaagcagac actggaaaag atagcggagg tcgaaagacg gtcgctttcg
     3541 tccataatag agaacatgct ccactcgcag gtcaaggaca aggttgaatt gaagggctcc
     3601 aaaaaggagc gcaggcggta cgcccgcaag gaggtatcgc ttcccgcctt cgtctttaag
     3661 cgggacgcga aggagtcgac cttgcagacg ggcatcgtca tagatgtctc gctggggggg
     3721 ctgaagatat ccattcccca cgattatgac gcggtcagcg agggtgagtt cgacaccatc
     3781 tttaccattc ccaacgagaa gaccccggta aagatgcgct gcaccataaa gagaatagcc
     3841 gaaggcgaag aagccacgaa ggaactcggg gcggacttca tagacggcga tttcctgagt
     3901 taccagaagc tgcacaaata tctgtcctaa atgcaggtca tgggtcatgg gtcatgggca
     3961 acagggacat ctttttgcga gttcttccca taacccacaa cctatcaccc attacccctt
     4021 ttttgcatat ttcttcaaat aatcgaggtc ctcttccgta aagccttccc aggcataggg
     4081 gaagagcgcg ttgttctgga agatggcgtc gtcggccacg ggaaacctgg cggaatcctt
     4141 gtgcttgtcg aagagcggcg tgtgggggat cggcgtgtat tcggcaatgg atggtgtgac
     4201 gtcgagggcc ttgagataat cgatggcccg cctcacctcc tgccagggct gtcccgggag
     4261 gccggcgagg atataggccc tgatctggcc cctgccgtac cccgcccgcc tgagggcgcc
     4321 tacagccctt tcgaagaggg cgcccgtcac ctttcccccg gtctgtgcct gcctgccggg
     4381 atcgaccgat tccagtccga tccggacctc gcgaaagccc gagctgacaa gcaggttggc
     4441 cacctcgtcg tctatgaagg cggcgttgag ggcgttgggg ttgtatatgt ccacagggaa
     4501 gggaaggccg gcgatcctct cgagaagggg ttttgcgtga cgcgaactgt cgaaaaggaa
     4561 gctgtcgtca tagacgacga agcggcggac gcccctgtcg tgccagtgaa gtatctcgtc
     4621 cacaacggaa tcgacgccgc gccgcgccat tgccggatac atgtaggacg tggcgcagta
     4681 ggtgcagcgg aacgcgcacc cgagcgacgt cagcaggggc acgaaaggga tgtcccggca
     4741 gaggtcgtgt gcgggatagg gcaggatgtc gaggttgtcc atgtgggggg tgaagccggg
     4801 ccgtcgtccc gtcgcggctt cgacgtaccc gtagaagtcc cccagctccc cggaagaaac
     4861 gacgaggtcc gccgccgcga acctcaggga ggcctgctcc cggcacaggg tggggtatat
     4921 gccgccaacg actatcttcg accgggggtg tacccggcgt gcgagatcga cgacctccat
     4981 cgcgcccacg taccagtagg tcatgatgga ggttacgagg atgatgtccg gcggttccat
     5041 gccttcgaga agggccgaca gctcttcggg tggcatcccg tacctcctga gggtctttct
     5101 cacccccttg agcgcgtcgg gtttcggcac cttgtgtttg cggaagggcg cgcgcccgtc
     5161 aggtttgcgc ttctcttcgc tgacggacat gcagtcgatg agatccacgt gaaatccgtt
     5221 gcgcctcagg atggccgcaa t
//
LOCUS       DAOPOA010000064         5167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17546_length_5167_cov_4.085094, whole genome shotgun sequence.
ACCESSION   DAOPOA010000064 DAOPOA010000000
VERSION     DAOPOA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17546_length_5167_cov_4.085094"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="PKJ17_02285"
     CDS             complement(<1..1711)
                     /locus_tag="PKJ17_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD74825.1"
                     /translation="MHREDEPEKGPKDHVSEGLKTACNVSPEKTAKLLEDLRLHQVEL
                     EMQNEELKKAQQVIEEGRQRLADLYDFAPVGYLTLNAQNVVEEANLTAATLLGTVRQV
                     LVGASFLQYVDSHYAAEYRRHHERVVKSRAKAVCELRLRRRGGSVFWARMESVPVTPG
                     FDDGDPVLLRTVFTDITERKEAEEALLEGEEKYRVLVESVNSIIARLDGEGRVIFLND
                     YGRDFFGYSEEELWGTGLVGTILPPGQYGLDAKEFLIYMADHPEDYREREIEMVRRNG
                     EKVWVSWMVRTLYDEKGVFTGMLAVGNDVTERRRLEGEIRQAQKMEAIGTLAGGIAHD
                     FNNILAGIIGFAEMIEEDSLPGSKDARRAQRIITAASRGADLVRQILAFSRKAEIARE
                     PLSLSPLIGETVRFLRSSLPATIEIQLAVKASDDTVLASPSEIQQILMNLVTNASFAM
                     MDTGGIIRAVITNVVFEPESPILDPDIEPGEYVQISVQDSGSGMSPDIMQRIFEPFFT
                     TKGVGQGTGMGLAVVFGIVKSLHGAISVESERGIGSTFRVLLPMARAAKKDEDPEAVL
                     PPGG"
     gene            complement(1723..4731)
                     /locus_tag="PKJ17_02290"
     CDS             complement(1723..4731)
                     /locus_tag="PKJ17_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOD74826.1"
                     /translation="MMATKETGKKKRTKTKQPAKGVPQPTEQAVVKKSFPIVALGASA
                     GGLEAFEVFFRSLPRTNEAAFVVISHLDPKHASMMSELISRFTRMEVSEAANDMEVGA
                     GHVYVIPPNRNLSISQGRLQLTEQEKSLGMKMPIDAFLRTLADDQGENSVAIILSGTG
                     TDGTLGVRALQAAGGVTFVQDPSEARYDGMPQSAIQTGLADYVLPVEEIARQLSVLFR
                     RYPVGREELPDDAMPNVIQKVLSVVRAKTGHDFSLYKKNTMNRRIRRRMSINNIQDGS
                     DYVRYLREHPEEVEQLFKELLITVTNFFREPEAFDNLKNTILPGIFKDKPEDYTVRVW
                     VPGCATGEEAYSIAIVIREYAEETGRECRLQMFATDMDETSIAQARTGLFPSNIAADV
                     TPARLAKYFVKEEAGYRVRKDIREGIVFATQDVAKDAPFTRLDLVSCRNLLIYMESEL
                     QSKIITIFHYSLKTNGILFLGSSESVGTRTDLFRVIDRKWKYYQAKPASGHEAVLHAA
                     FASWPPEHVRVDRPSPVVVRKADLEETVRTALLASFAPPAIVVNDKGDILYIYGDTTK
                     YLTPSPGRPSLNITHMTREGLRFPMRSALMAAATHQKEAVYRNVKVKVNGTTMTIDLA
                     IRPLPRAEEEEALFMFTFGEVSEEQAAERTSDERDVDREHLLDLEKELIYTRESLQAA
                     AEEAQAANEELKSANEELQSSNEELQSTNEELETSKEELQSVNEELTTVNAELRSKIE
                     QLSQSENDMKNLLDSTDIATVFLDNNLNIKRFNSAATKVVSLIPSDAGRPVGDVTMKI
                     EYPDLPNSARDVINRLRPFEAEVRSKEDRWFVMRIVPYRTLDNVIDGVVMTFTDITES
                     KCAADERARFFEHIVQTVREPFLVLNEDLRVVMANRSFLDFFKVRRDETEGRVIRDLG
                     KREWDIPVLRGFLSDVIKTDEVFEDFRVEADFPSIGHHVLVVNARKIKATVASDEPLI
                     LLAMEDFTSKDCFPGETGTGKNPEGKRKSE"
BASE COUNT          991 a         1579 c         1443 g         1154 t
ORIGIN      
        1 tccccccggg gggcaggacc gcttcaggat cttcatcttt tttagcggcc cttgccatgg
       61 ggaggagaac ccggaacgtg gaaccgatcc cccgttcgct ctcaacgctt atggcgccat
      121 ggaggctctt cacgatgccg aagacaaccg ccaggcccat gcccgtcccc tggccgacac
      181 cttttgtggt aaagaaaggt tcgaagattc gttgcattat gtcggggctc ataccggagc
      241 ctgaatcctg cacggagatc tgcacatatt cgccgggttc aatgtccgga tcgaggatag
      301 gggattcggg ctcgaagacg acattcgtga tcactgccct gatgatcccg cccgtgtcca
      361 tcatggcaaa agaggcattg gtaacaagat tcatcaggat ctgctgtatc tctgaaggag
      421 acgccagtac ggtgtcatcg gacgccttga cggcgagttg gatctcgatc gtggcaggga
      481 gagacgaccg caagaaacgc accgtttccc cgatcagggg tgaaagcgac aggggctccc
      541 tggcaatctc ggccttgcgg gaaaaggcca gtatctggcg gacaagatcg gccccgcgcg
      601 aggcggcggt tattatcctt tgtgcccgcc gggcatcctt gctgccgggg agggaatcct
      661 cctcgatcat ctccgcgaat cctatgatgc ccgcaaggat gttgttgaaa tcatgggcga
      721 tgcccccggc gagagtgccg atggcctcca tcttctgcgc ctgccggatc tcaccttcca
      781 gcctcctgcg ttcggtgaca tcgtttccca ccgcaagcat cccggtgaaa acaccctttt
      841 catcgtacag tgtccggacc atccacgaga cccagacctt ttccccattg cgtcggacca
      901 tctcgatctc tctttcccga taatcctcgg gatggtccgc catgtagatg agaaattcct
      961 ttgcgtccaa accgtactgc ccgggaggca atatcgtccc cacgagtccc gttccccaca
     1021 attcctcctc ggagtaaccg aagaagtccc gcccgtaatc attcaggaag atgacgcggc
     1081 cctcgccgtc gagccgcgcg atgatgctgt tcacgctttc gacaagtacc cggtacttct
     1141 cctcgccctc aaggagcgcc tcctccgcct ctttcctttc agtgatgtct gtgaataccg
     1201 tgcgtagcag gacgggatcg ccatcatcaa aaccaggcgt gacgggaacg ctctccatgc
     1261 gggcccagaa gacacttccg ccgcgtcttc gcaaccgcag ttcgcaaact gcctttgccc
     1321 tgctcttcac gaccctttcg tggtgccgac gatactcggc ggcatagtgg gaatccacgt
     1381 actggaggaa gctcgcgccg acaaggacct gcctcaccgt gccgagcaag gtggcggcgg
     1441 tgaggttcgc ctcttccacc acgttctggg cgttgagggt taggtagccc acgggcgcaa
     1501 aatcgtagag gtcggccagc cgctgccgcc cctcttcgat gacctgctgg gccttcttga
     1561 gttcctcgtt ctgcatctcc agttcgacct ggtggaggcg caggtcttca aggagtttgg
     1621 ccgtcttctc cggggaaacg ttgcaagcgg ttttcaggcc ttcggagaca tggtccttcg
     1681 gacccttttc tggttcatct tcccgatgca tcgctgcctc gcctattcgc tcttcctttt
     1741 cccttccggg ttctttccgg ttcccgtctc gccgggaaag cagtctttgc tggtaaaatc
     1801 ttccatggca agcagaatca gaggctcatc cgacgcgaca gttgctttta tcttccgggc
     1861 attcacgacc aggacatgat gaccgatgga gggaaaatcg gcctcaaccc tgaaatcctc
     1921 aaagacttcg tcggtcttga taacgtccga cagaaaccct ctcaggacag gaatgtccca
     1981 ctcgcgtttt cccaggtccc tgatgacccg gccctccgtc tcgtcccgcc tcaccttgaa
     2041 gaagtcgaga aagctcctgt ttgccatgac cacccggagg tcttcgttga gcacgagaaa
     2101 tggttcccgc acagtctgca cgatgtgctc gaagaatctc gcccgttcat ccgccgcaca
     2161 ctttgactcc gtgatgtcgg tgaaggtcat gacgactccg tcgatcacgt tgtccagcgt
     2221 ccggtaagga acgatccgca tgacaaacca cctgtcctcc tttgacctca cttccgcttc
     2281 gaagggccgc agtcggttga tcacgtcccg cgcgctgttg ggaagatcag ggtattcgat
     2341 cttcatggtc acgtcgccga caggccggcc cgcatcggaa gggatgaggc tgacaacctt
     2401 ggtggcagcg gaattgaacc gcttgatatt aaggttattg tcaaggaata ccgtagcgat
     2461 atcggtgctg tcgagaaggt tcttcatgtc gttctcgctc tgggaaagct gttcgatctt
     2521 ggacctgagc tccgcgttga ccgtcgtcaa ctcctcgttc acggactgaa gctcctcctt
     2581 tgaggtctcc aactcctcgt tggtggactg gagttcctcg ttgctcgact gcaattcctc
     2641 gttggcggac ttgagttcct cgttggccgc ctgcgcctcc tcggccgcag cctgcaggct
     2701 ctccctcgtg tatatgagct ccttttccag atccagaagg tgctccctgt cgacatcccg
     2761 ttcgtcactc gtcctctccg ccgcctgttc ctcgctgact tccccgaagg tgaacatgaa
     2821 gagcgcctcc tcctcttcgg cccttggcag gggacggatg gcaagatcga tcgtcatcgt
     2881 ggtcccgtta accttgacct tgacgttcct gtacaccgcc tccttttgat gcgtcgcggc
     2941 ggccatcagg gccgagcgca tgggaaagcg caagccctcc cgcgtcatgt gggtaatgtt
     3001 gagcgagggg cgacccggtg atggagtcag gtactttgtc gtatcgccgt agatatagag
     3061 tatgtctccc ttgtcgttca cgacgatcgc cggaggcgca aaggatgcga ggagcgcggt
     3121 gcgcaccgtc tcttccaggt cggccttccg caccaccacc ggagacgggc ggtccactcg
     3181 gacgtgctcg gggggccagg aggcgaaggc cgcgtggagg acggcctcgt gacctgaggc
     3241 cggtttcgcc tggtagtact tccatttcct gtcgatcacc cggaaaaggt ccgtccgggt
     3301 accgacgctc tcggaagaac caagaaagag gatgccgttc gtcttgagac tgtagtggaa
     3361 gatggtgatt atcttgctct ggagctccga ttccatgtag atgaggaggt tcctgcagga
     3421 tacaaggtcg agcctggtga agggcgcgtc cttggccaca tcctgggtcg cgaagacgat
     3481 cccctccctg atgtccttgc gcacccggta gcccgcctcc tccttgacga agtactttgc
     3541 caggcgggca ggggtcacat ccgcggctat gttggaggga aagaggcccg tgcgggcctg
     3601 cgctatcgag gtctcgtcca tgtcggtggc gaacatctgg agcctgcatt cgcggcccgt
     3661 ttcctcggcg tactcccgga tcacgatggc gatggaatag gcctcctcgc cggtggcgca
     3721 gccgggcacc cacacccgca cggtgtaatc ctccggtttg tccttgaaaa taccgggaag
     3781 gatcgtattc ttgaggttgt caaaggcctc cggttcgcgg aagaagttcg tcacggtgat
     3841 gagaagctcc ttgaaaagct gctccacctc ctccggatgc tcccgcagat agcgaacgta
     3901 atcggacccg tcctgtatgt tgttgatact catccggcgc cggatcctgc ggttcatggt
     3961 gttcttcttg tagagggaga aatcatgccc tgtctttgcc cgcaccaccg acagcacctt
     4021 ctggatcacg ttcggcatgg cgtcatcggg cagttcttcc ctcccgacag gataccgtcg
     4081 aaagagaacc gagagctgcc tggcgatctc ttccacgggc agaacataat cggcgagacc
     4141 cgtctgtatg gcgctttgcg gcatgccgtc gtatcgcgct tcagaagggt cctgaacgaa
     4201 ggtcacgccg cccgccgcct ggagggccct caccccgaga gtcccgtccg tgcccgtgcc
     4261 ggaaaggatg atggccacgg aattctcacc ctgatcatct gccagggtgc gcagaaaagc
     4321 atcgatgggc attttcatac caagggactt ctcctgttcc gtgagctgga gccttccctg
     4381 ggagatcgac aggttcctgt tgggcgggat gacgtagaca tgccccgccc ccacctccat
     4441 gtcgtttgcg gcctcgctga cctccatccg cgtgaatcgg ctgatcagtt ccgacatcat
     4501 gctcgcatgc ttgggatcaa ggtgggagat caccacgaaa gcggcctcgt tcgtccgggg
     4561 aaggctcctg aagaacacct cgaacgcctc aagcccccct gccgacgcgc caagggccac
     4621 tatgggaaag gatttcttca caacggcctg ctccgtcggt tgcggcaccc ctttcgctgg
     4681 ctgcttagtc tttgtgcgtt tcttcttgcc tgtctctttg gttgccatca tcacccccct
     4741 gagtgaccgt cttctgcaga aacatccgga cggtcaaccc aacaatgata acacaagtat
     4801 aaacgccttt acgggatata gcaatcaccg ctcgaaccac atagatctcg aacagaagcg
     4861 ccctggcggg acagccctca aggcggcacc tttttccatt gacctcttcc cgcgatgaga
     4921 cgaaaatgaa ggtgtggaac ttgaatcgtg cgtggcaggg gctttaagtc cctgaaaaca
     4981 gcaaggaggc ctggaatgaa ggtagttcgt tttgaaagtg ccgaaagcta tgagccggaa
     5041 aaggactgga agcgggtgag cctctgcgcg gaaaaggata tctccatcga acacttcgtg
     5101 aaaccgccgc gtcacgcgtc gccgaaccac gaccatccaa actcgcaggt gttgatagtc
     5161 ctgaagg
//
LOCUS       DAOPOA010000065         5160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17571_length_5160_cov_3.728697, whole genome shotgun sequence.
ACCESSION   DAOPOA010000065 DAOPOA010000000
VERSION     DAOPOA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17571_length_5160_cov_3.728697"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2276)
                     /locus_tag="PKJ17_02295"
     CDS             complement(<1..2276)
                     /locus_tag="PKJ17_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOD74827.1"
                     /translation="MRKSSAMMAVLLTGLLSCTVAHGGTVWDGAGGNTNINTAGNWDD
                     NVNPPFDGTVSVAFGTGGSTATINTNVSFLGLIFNRDSNFTVAAGAGTLTIGAGGIDA
                     AIPGTTSRTYTITESVFLGADQTWTATNNGAGTTALTVSGAVSGTGRNLAVAGTGATN
                     ISGVIGTGSGSLTKTGTGVLTLTGTNTYSGGTALLDGTLRVGNNSAFGTGTLTIAGGT
                     LSSSSGTARTIANDIVVGADFILGQSSGGTGALTLTGAMDLGGAVRQITVNNSADTIS
                     GVISGTGGITKAGTGTLTLSNANTYSGGTTLSSGILLAGDDGAFGTGTLTIAGGTLSS
                     SSGTARTIANDIVVGADFILGQSSGGTGALTLTGAMDLGGAVRQITVNNSADTISGVI
                     SGTGGITKAGTGTLTLSGNNAYSGGTTVKAGRLSVESDSNLGDASGGLTFAGGTLQMT
                     GSFASGRDVTLNSGGGTFDTNGYDLTMSGGMTGVGTLTKTGSGTLTLTGTNTYTGTTT
                     ISGGVLQAGVDNALPTATAVTLSNTAGVALDINNFNVTIGSLAGGGTTGGNVTLGSGT
                     LTVGNTSNTTYAGIISGTGLLVKTGTGALTLSGANTYSGGTTLSSGSLLLGNNAALGS
                     GTLTFSGGTLASSSSTARTIANNSTLNGDTTLGQASGGTGALTLSGAMDLGGSMRQIT
                     IANTTDTISGIISGAGGITKAGTGTLILTGANTYTGGTILSTGTLLAGDGSAFGTGTL
                     TLNGGTLASSSSTARTIA"
     gene            complement(2697..3848)
                     /locus_tag="PKJ17_02300"
     CDS             complement(2697..3848)
                     /locus_tag="PKJ17_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD74828.1"
                     /translation="MIERRSWIKRIEEVWEETPIVWLSGVKGVGKTTLIQGLGTQRTL
                     YVDCDPARSAHIAADPEFFFRACDRPVVVFDDVHKLPDAMKILKTGADLFPELRIIAT
                     SSTVAADGKAGDVLGDRKRRVHLGPVLWNELPAFNVSLERRFYHGGLPEALLTEHRQG
                     GFYREWMDAFFDRDIREPFGFRDSDKFTMLFKYLLKQSGEPFETTRASETLRISRPTV
                     AEHVRALDATGAITVVRPFHGKGQKEIIKMPRVYGFDTGFVAFARGWDPLRPDDYGPL
                     WEHIVLEFLETHVRGSQVQYWRWADDRKVDFIVPGTRDEVDTVECEWNPDAFDPAGLK
                     LFRTWYPEGKNYIISPLSSAAVERHIAGLDVHVCDLEGWLERQGASRKD"
     gene            4074..4493
                     /locus_tag="PKJ17_02305"
     CDS             4074..4493
                     /locus_tag="PKJ17_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOD74829.1"
                     /translation="MKIVNEHREFSTQGSGDLIDITRDVAGCLVGSGLTNGSMTIFVQ
                     GSTAGITTFEYEPGLIGDVQEFYERMVPSDRAYRHDETWGDANGFSHLRATLTGQSLT
                     VPFESGVLLLGTWQQVVLAEFDNRPRERLVVVQMIGE"
     gene            4591..5070
                     /locus_tag="PKJ17_02310"
     CDS             4591..5070
                     /locus_tag="PKJ17_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74830.1"
                     /translation="MCRLLPLKSIALFLGILLSFLSLGGCRKPEPARVILAVDGEMFS
                     DALVIIDGKQAGKLTQTLIKPDGNLYIDGILTAKLPPGHGDIREKDDYQGTIDSLELM
                     AGEHTILLQTEDGKTLQITARIPPGRHLVTYLSNEETLTWNSAKLKAPPGSSVNVRP"
BASE COUNT          979 a         1570 c         1590 g         1021 t
ORIGIN      
        1 ttcgctatcg tccttgccgt cgaagaagag gaagcgaggg tgccgccgtt aagcgtcagt
       61 gtaccggtcc cgaaagcgga gccatcaccg gcgagaagcg tccccgttga gagtatcgtt
      121 ccccccgtgt aggtgttggc gccggtgagg atgagcgtgc ccgtgcccgc cttcgtgatg
      181 ccccctgccc ccgagatgat ccccgagatg gtgtccgtcg tattggctat cgtgatctgc
      241 cgcatggacc cgccgaggtc catcgccccc gagagggtga gcgcgcccgt gccacccgat
      301 gcctggccga gggtagtatc cccgttgagg gtgctgttgt tggcaatcgt ccgcgccgtc
      361 gaagaagagg aagcgagggt gccgccggag aatgtgaggg ttcccgagcc gagggcggcg
      421 ttgttgccga gaaggaggct cccggaagag agggtcgtgc cgccgctgta tgtattggcg
      481 ccggagaggg tcaatgcacc cgtgcccgtc ttgacgagca agcccgtgcc gctgatgatc
      541 ccggcatagg tggtgttgga agtgttgccc accgtgagcg tgcccgagcc gagggtgacg
      601 ttgccccccg tcgtgccgcc ccccgcgagg gagccgatcg tcacgttgaa gttgttgatg
      661 tcgagggcga cacctgccgt gttggagagc gtcaccgccg tcgccgtggg gagggcgttg
      721 tcgacgccgg cctggagcac gcccccgctt atcgtcgtcg tgcccgtgta ggtgttcgtt
      781 cccgtaaggg tgagggtccc cgagccggtc ttggtcaggg tgccaacgcc tgtcatgccg
      841 cctgacatgg tcaggtcata accgttggta tcgaaggtac cacctccgga attgagggtc
      901 acatccctgc cgctggcaaa gctccccgtc atctggaggg tcccgccagc gaaggtgagc
      961 ccccctgacg catcccccag gttgctgtcg ctctctacgc tcagtcttcc tgccttgacg
     1021 gtggtgccgc cggaataggc gttgttcccc gacaacgtca gggttcccgt gcctgccttc
     1081 gtgatgccgc cggtccccga tatgacaccc gagatggtat ccgccgaatt attgacggtg
     1141 atctgccgca ccgcgcctcc gaggtccatg gcgccggtga gcgtgagcgc gccggtgccg
     1201 cccgatgact ggccgaggat gaagtcggcc ccgaccacga tgtcgttggc tatcgttctg
     1261 gccgttcccg aggacgaaga aagggtgccg ccggcgatcg tgagcgtgcc cgtgccgaac
     1321 gcgccatcgt caccagcgag aagtatcccc gatgaaaggg tcgttccccc cgaatacgtg
     1381 ttggcattcg agagcgtgag cgtacccgtg cctgccttcg tgatgccgcc ggtccccgat
     1441 atgacacccg agatggtatc cgccgaatta ttgacggtga tctgccgcac cgcgcctccg
     1501 aggtccatgg cgccggtgag cgtgagcgcg ccggtgccgc ccgatgactg gccgaggatg
     1561 aagtcggccc cgaccacgat gtcgttggct atcgttctgg ccgttcccga ggacgaagaa
     1621 agggtgccgc cggcgatcgt gagcgtgccc gtgccgaacg cagagttgtt ccccaccctg
     1681 agtgtcccgt cgagcagcgc cgtgccccct gagtacgtgt tcgttcccgt gagagtgaga
     1741 acaccggtac ccgtcttcgt taggctgccc gaaccggtgc cgatgacacc ggagatgttc
     1801 gtcgcgcccg tgcctgccac cgcgaggtta cggccagtcc ctgacaccgc accgcttacg
     1861 gtcagggccg tcgtcccggc gccgttgttc gttgccgtcc acgtttggtc cgcaccgagg
     1921 aacacgctct cggtgatggt gtaggtccgt gatgtggtgc cgggaatggc tgcatcgatg
     1981 ccccccgccc cgatggtcag ggtcccggcg cctgccgcaa cggtaaaatt gctgtcacgg
     2041 ttgaagataa ggccgagaaa actgacattg gtgtttatgg tggccgtgga cccgcctgtc
     2101 ccgaatgcca cggacacggt gccgtcaaag ggagggttga cgttgtcatc ccagttgccg
     2161 gccgtgttga tgttggtatt cccccccgcg ccgtcccaga cggtccctcc gtgagcgacc
     2221 gtgcaggaca ggaggcctgt gagcagaacc gccatcatgg ccgagctttt tctcatatcg
     2281 gctgcacagc ctctccgtta cccgggttgt cgcggttgat ggtctcaggt gtgcgaactc
     2341 tgacaactcc ccgccgcacg ggcggggacc tctgctgttg taccattcag atacaacggg
     2401 gaggaaactt cctcattggc ctcattgttc tacaatttta tatgtattac aagagaaatc
     2461 atacaggcga agacactcgg ctgtgacgac cgccggagct cttctcgaag ggcctctgcc
     2521 gcaccttcat cgatgaatcc aggaggagcc aacagctcgt cacgcagatc gagaaggcag
     2581 cctcccgcct cacattcctg aaccacgtca taaggggaaa ggaccaggag gaattctgcc
     2641 tctttttcgg gaggctgcga gggagcatct cgttagcgaa atacgttttc ccccggctag
     2701 tctttgcggc tggcgccctg cctttcgagc catccctcaa ggtcgcagac gtgaacgtcg
     2761 agtccggcga tgtgtctttc aacggccgcg ctcgacaggg gagagatgat gtagttcttt
     2821 ccttcgggat accaggtgcg gaagagcttg agccccgcgg ggtcgaaggc gtcggggttc
     2881 cactcgcatt cgacggtatc cacctcatcc cgcgtgccgg ggacgatgaa atccaccttt
     2941 cggtcgtccg cccatctcca gtactggacc tgggacccac ggacgtgggt ttcaagaaac
     3001 tcgaggacga tatgctccca gaggggaccg tagtcgtcgg gacgcagcgg gtcccaaccc
     3061 ctggcaaagg ccacgaaacc cgtgtcgaac ccgtagaccc tgggcatctt tattatctcc
     3121 ttctgccctt ttccgtggaa aggccggacc acggttatcg ccccggtggc atcgagggcc
     3181 cttacgtgct ccgcgacggt gggacggctg atgcggaggg tctccgatgc ccgtgtcgtc
     3241 tcgaagggct cgccgctctg tttcagcaga tatttgaaga gcatggtaaa cttgtcggaa
     3301 tcacgaaacc cgaagggctc gcggatatcg cggtcaaaaa aggcgtccat ccattcacga
     3361 tagaacccgc cctgccggtg ttccgtcaaa agggcctcgg ggagcccgcc atgatagaat
     3421 cgcctctcca gagaaacgtt gaaagcggga agctcgttcc acagaacggg gccaaggtgg
     3481 acgcggcgct tcctgtcacc gagtacgtca ccggccttcc cgtcggccgc cacggtggac
     3541 gaggttgcaa tgatcctgag ctccggaaaa agatcggctc ccgtcttgag gatcttcatg
     3601 gcgtccggga gcttgtggac atcgtcaaag accacgaccg ggcggtcaca ggcgcggaag
     3661 aagaactcgg ggtccgctgc tatgtgcgcg gaccgggccg ggtcgcagtc gacgtagagc
     3721 gtccgctgcg tcccgagacc ctgtatcagg gtcgtcttgc ccaccccttt cacgccggaa
     3781 agccacacaa tgggcgtctc ctcccagacc tcttctattc tctttatcca ggatctccgt
     3841 tctatcattg agacaacaca gttaccatat attcggaata aacgtcaagt agagtgtacg
     3901 aaagacaggg cgcccgatgc ggcccttgct ctcaaaaccc ttccaatgcg cccctctctc
     3961 tggtagggta atgaaggcag tcatgggcaa tggagttata ggtgatgggg ggaaggtccg
     4021 gtctttccca tcacccatga tctattacct gtattctcac gagaggtgat ttcatgaaga
     4081 tagtgaacga acacagggaa ttttcaaccc agggaagcgg agaccttatc gacatcacca
     4141 gggacgtggc cggctgcctc gtggggagcg gactgacgaa cggcagcatg acgatcttcg
     4201 tgcagggctc cacggcgggc atcaccacct tcgagtatga accgggcctt atcggcgacg
     4261 tgcaggaatt ctacgaaagg atggtccctt ctgatcgcgc gtaccgccac gacgagacat
     4321 ggggtgacgc gaacggattc tcccacctca gagccacgct gacaggccag tccctgacgg
     4381 tgcctttcga atcgggcgtt ctgcttctcg gcacctggca gcaggtggtc ctcgccgaat
     4441 tcgacaaccg cccgcgggaa cggctggtgg tggtgcagat gataggggaa tgaaacggtt
     4501 gcaacccatt cttttctgga ctatactgag tcgtatttca ccgtgcgtac gctgtaataa
     4561 ggaaggatct tcgagaatcg gggaaatctc atgtgccgtc tgttgccgct gaagtccatc
     4621 gccctcttcc ttggaattct cctgtcgttc ctttccctgg gaggttgcag gaaacccgag
     4681 cctgcccggg tcatccttgc cgtcgatgga gagatgttct cggacgccct tgttatcatc
     4741 gacgggaagc aggccgggaa gctcacgcaa acactcatca agcccgatgg aaacctgtac
     4801 attgacggca tcctcaccgc aaagctgccg cccggccacg gggacatccg ggagaaggac
     4861 gattaccagg gcacgatcga ttccctggag ctcatggccg gcgagcacac catcctcctc
     4921 caaacggaag acgggaagac gctgcagatc acggccagga taccccccgg ccgccacctc
     4981 gtaacctacc tctccaacga ggaaaccctc acgtggaaca gcgcaaaact caaggccccc
     5041 ccggggtcaa gcgtgaacgt aagaccgtaa agacggctgc aaccgcttga accgcttgaa
     5101 cgttgaagag ctcagaaatg agaaggcttg tgaggatacc gggcttgcgg cggaacggag
//
LOCUS       DAOPOA010000066         5141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17651_length_5141_cov_2.713134, whole genome shotgun sequence.
ACCESSION   DAOPOA010000066 DAOPOA010000000
VERSION     DAOPOA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17651_length_5141_cov_2.713134"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PKJ17_02315"
     CDS             complement(<1..253)
                     /locus_tag="PKJ17_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOD74831.1"
                     /translation="MNIQIYGTSKCQDTRKAQRFFKERGIPFQYIDLTVKGLSRGELN
                     SVKAAVGIENLMDTQGQRYVRRNLTYLTHDPEEELLGDPL"
     gene            complement(270..2090)
                     /gene="typA"
                     /locus_tag="PKJ17_02320"
     CDS             complement(270..2090)
                     /gene="typA"
                     /locus_tag="PKJ17_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOD74832.1"
                     /translation="MEQNKIRNIAIIAHVDHGKTTLVDQFFRQSGMFRHNEIVEERLM
                     DSMDLERERGITISSKNGSFAYKDFFINIIDTPGHADFGGQVERVLKMADGVLLLVDA
                     AEGPMPQTRFVLKKALNLNLPVIVVVNKIDKPAARCDWVVDQVFDLFVKLDAPDDILD
                     FPIIYTSAKAGYATLDHTQAGGSMLPLFDMITDSVPPPMGNPDAPLQMLVSSLSYSPF
                     LGRLAIGKITSGVLNLNKDVIVATEGVPSAPARITKVYRFKGSGMEETEIAGTGEIVA
                     VAGMDSITIGETLTDPENPAPLPGIDIDPPTISMNFIPNDSPFAGKEGRFLTSRHIRE
                     RLMRETLSDVALVVEELSDSIGYKVSGRGELHLSILIEKMRREGYEFQVTRPKVIFKD
                     SDDGVLEPYEELTIDVDEEYSGNVIENLGRRKGQMVHMHQDKGMVRLSYRVPTRGLIG
                     FRSQFLTETKGTGTMNYIFDGYDRYAGEMKNRNNGVLVVMEECTTVAYALFNLQERGE
                     LFLGPGEKVYGGQIIGEHTREIDLVINPAKGKKLTNMRAAGSDDAVILTPPRYMSLED
                     CISYINEDELVEITPRSIRLRKTILDGLQRKRSKIAGQKA"
     gene            complement(2144..2344)
                     /locus_tag="PKJ17_02325"
     CDS             complement(2144..2344)
                     /locus_tag="PKJ17_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOD74833.1"
                     /translation="MAKGTVKWFNESKGFGFITGDDGTDVFVHYSAIQGSGFKSLSEG
                     EQVTFDVVNGPKGPAASNVAKA"
     gene            2642..3433
                     /locus_tag="PKJ17_02330"
     CDS             2642..3433
                     /locus_tag="PKJ17_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD74834.1"
                     /translation="MTDLDRGLRSYDRPEILYRLFFPRRDVAPDSESPRAFNRFVEVA
                     EGVSICCRCYPARPDGPNILYFHGNGEVAADYDHVAPVYAERGLNLFVADYRGYGGSG
                     GRPTCTAMIKDAHPVFRDFMEYIGKNGLTGSPFVMGRSLGSAPAIELAYHYQAEFEGL
                     IVESGFARAANQVARLGVEHLFDDEDPVGFGNDLKIADVKIPTLIIHGEWDQIIPASE
                     GKTLYELSGAAGKSSLFIPRAGHNDIMMLGLKAYMDAVQAFTRRA"
     gene            3552..4379
                     /locus_tag="PKJ17_02335"
     CDS             3552..4379
                     /locus_tag="PKJ17_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOD74835.1"
                     /translation="MAHKPVEIVQLAGDAGKYKANLTPGNYLVRSFMAGLFIAVGGAL
                     ATVCSTGLENSIGPGFKSMIAGSVFPVGLIAIVLTGMSLFTGDTMLIPMAVFQNKSTW
                     GKVINAWFWVYIGNFIGSLFWAYLMTVGPFSKGGGAELTAFGQSAVAIAQAKTLPYMA
                     NGGAGLWSAFMKGIGCNLLVNLAILLALSSKNMIGKFFGIWFPIMAFVSSGFEHCVAN
                     MYFIPAGIMLGANVTWGQFIGWNLIPVTIGNIVGGFVFIGAVYFISFKKELSTITPT"
     gene            complement(4445..>5141)
                     /locus_tag="PKJ17_02340"
     CDS             complement(4445..>5141)
                     /locus_tag="PKJ17_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD74836.1"
                     /translation="RRAFNDRTRAILINTPHNPTGHIFTMEELEGIAELCREFNVVAV
                     TDEIYEHIIYDGQPHVPIATIPGMEERTVTISGLGKTFAMTGWRLGYACALNPLSTIL
                     RTVHDFMTICAPAPLQAACAAVLALPDEYYTGLRRDYTLRRDKMISILQRAGFSAHSP
                     EGAFYAMADFSAWDFDGDDYSFARWLPANLGVAVVPGSCFYGTKGMGKKTVRFAFAKK
                     METLEAAEARLCS"
BASE COUNT         1001 a         1401 c         1563 g         1176 t
ORIGIN      
        1 ggagggggtc gccgagcagc tcttcctccg ggtcatgggt gagatacgtg aggtttcgcc
       61 ggacgtagcg ttgcccctgc gtgtccatga ggttctcgat gcccacggcg gccttgacac
      121 tgttgagctc acccctgctc aggcccttga ccgtcaggtc gatgtactga aatgggatac
      181 cccgctcctt gaagaaccgt tgggcttttc gtgtgtcctg gcatttggag gtgccgtata
      241 tctggatgtt catggcgcga cggggtttgt caggccttct ggccggctat tttcgatctc
      301 ttgcgctgga gcccgtcgag gatggtcttg cgcagtctga tggacctcgg tgtgatctcc
      361 acgagctcat cttcgttgat atatgagata cagtcttcca ggctcatgta ccggggaggc
      421 gtgaggatga cggcgtcgtc ggagccggcc gccctcatgt tcgtcagttt cttgcccttg
      481 gcggggttta tcacgaggtc aatctcccgc gtgtgttcac cgatgatctg gccgccgtag
      541 accttttcac cgggaccgag aaagagttcc ccgcgctcct gcaggttgaa aagggcgtag
      601 gccacggtgg tgcattcctc catgaccacg aggaccccgt tgttgcggtt cttcatctcg
      661 ccggcatacc ggtcgtagcc gtcgaagatg tagttcatgg tgcccgttcc cttggtctcc
      721 gtcaggaact gggagcggaa accgatgagg ccccgcgtgg ggacgcggta gctcagcctc
      781 accatgccct tatcctggtg catgtggacc atctggcctt tgcgccgccc gagattctct
      841 atgacattgc cggagtactc ctcgtcgacg tcgatggtca gttcctcgta gggttcaagg
      901 accccgtcgt cactgtcctt gaatatgacc ttcgggcggg tgacctggaa ttcgtaaccc
      961 tccctgcgca tcttctcgat gaggatggac aggtgaagct cgccccggcc cgacaccttg
     1021 tacccgatgg agtcactgag ttcctcgacg acgagggcca catcggacag tgtctctcgc
     1081 atgagcctct ccctgatgtg ccgcgacgtg aggaaccggc cttcctttcc ggcgaaaggg
     1141 gaatcgttgg gaatgaagtt catcgatatt gtcggggggt cgatatcgat gccgggaagg
     1201 ggcgccgggt tctcagggtc cgtcagcgtt tctccgatgg ttatggaatc catccccgcg
     1261 acggcgacta tctcaccggt gccggctatc tccgtctctt ccatgccgct tcccttgaaa
     1321 cggtagacct tcgttatccg ggccggtgcc gagggtaccc cttccgtggc tactatcaca
     1381 tccttgttga ggttgagaac gcccgaggtg atcttcccga tcgccagcct tcccagaaag
     1441 ggtgaataag agagggagct cacaagcatt tgaaggggag cgtcggggtt tcccatgggg
     1501 ggaggcacgg aatcggttat catgtcgaag aggggcagca tcgaaccgcc cgcctgcgtg
     1561 tggtcgagcg tggcgtagcc ggccttcgcc gatgtgtaga tgatcgggaa atccagtatg
     1621 tcgtcgggcg cgtcgagctt gacgaagagg tcgaagacct ggtccacgac ccagtcgcac
     1681 cgcgcggccg gcttatcgat cttgttgacg acgacgatga cgggcaggtt gaggttgagg
     1741 gccttcttca ggacgaacct cgtctgcggc atcggtcctt cagcggcatc gacaagcagg
     1801 aggactccat cggccatctt gaggacccgc tccacctggc caccgaaatc ggcatgcccc
     1861 ggggtgtcga tgatgttgat gaagaagtcc ttgtacgcga aggagccgtt ctttgaagag
     1921 atggtgatac cccgttcccg ttcgaggtcc atggagtcca tgagcctctc ctcgacgatc
     1981 tcgttgtggc ggaacatgcc gctttgtctg aagaattggt ccacaagggt ggtcttcccg
     2041 tggtcgacgt gggcaatgat cgcaatattc ctgatcttat tctgttccat cggggtgggg
     2101 atcgaaggca ggcccgactg tcgccgggcc tgcatcgggt ggtttacgct tttgcgacgt
     2161 tggatgcggc cgggcctttg gggccgttga ccacatcaaa ggtaacctgc tcgccttcgc
     2221 tcagggactt gaaaccgctg ccctgaatgg ctgagtaatg cacgaacacg tcggtgccgt
     2281 catcgcccgt aatgaaacca aaaccttttg actcgttgaa ccatttaaca gttccctttg
     2341 ccatgctaca aacctcctaa aggatattga aatgtggaag aaaaggaact gtgctatggg
     2401 acagacaaca aaaatgggaa attcctgcgt atgtgacgaa cagcgtgttc cttcctatgc
     2461 tccggggtcg attatctcac aaaaataagg ggagtgcaac aaatattctc gttggggccg
     2521 aaaaatatgg cacaagcggg ttttcttcct tgatatgaga tgacgaattc ctttaaccta
     2581 agaagggggc acggcgcgac cggcattctg ggatggtccg ggtcctgaag ggaggcgcgg
     2641 catgacagat cttgacagag ggcttcgatc ctatgaccgg ccggagatcc tctaccggct
     2701 tttctttcca aggcgcgatg tggcgcccga ttcggagagt ccgagagcgt tcaacaggtt
     2761 cgtggaggtg gcggagggcg tttcgatctg ctgccgctgc tatccggcac ggccggacgg
     2821 gccgaacatt ctctattttc acggcaacgg agaggttgcc gccgactatg accacgtggc
     2881 gcccgtctat gctgagcggg gattgaatct tttcgtcgcc gattaccggg gatatggggg
     2941 aagcggcgga cgtccaacgt gtacggccat gataaaggat gcccaccccg tcttccgtga
     3001 cttcatggag tatatcggca agaacggcct cacgggaagc cccttcgtta tgggcaggtc
     3061 tctcggaagc gccccggcca tcgagcttgc ctatcattac caggcggagt tcgaaggtct
     3121 catcgtggag agcggttttg cgagggccgc gaaccaggtg gcaagactgg gagtggagca
     3181 cctcttcgat gacgaggacc ctgtcgggtt cggcaatgac ctgaagatag cagacgtgaa
     3241 gatccccacg ctcatcatcc acggcgaatg ggaccagatc atccccgctt cggagggaaa
     3301 gacgttgtac gaactgtcgg gagctgccgg gaagtcctct ctctttattc cccgggccgg
     3361 tcacaacgac atcatgatgc tcgggctcaa ggcttacatg gatgccgttc aggcattcac
     3421 ccgaagggcc tgataaattg cgagaattaa ttacttgata tctgcccgct ttctgtggtt
     3481 ttcttaagaa aatcacaatc ggcacccagc agcaagacag aaggttgaga caaccccaaa
     3541 aaggagggat tatggcgcac aaacctgtgg agatcgtgca attggctgga gatgcaggca
     3601 agtacaaggc taatctcaca cctggcaatt atcttgtacg gagctttatg gcaggcctct
     3661 tcatcgctgt cggaggggcc cttgccacgg tatgttcgac gggacttgag aactccatag
     3721 gtccggggtt caagtcaatg atagcgggtt cggtcttccc tgtgggcttg atagccatcg
     3781 tcctcacggg catgtcgctt ttcaccggcg acacgatgct gatccccatg gcagtgttcc
     3841 agaacaagtc gacctgggga aaggttatca atgcctggtt ctgggtttac ataggtaatt
     3901 tcatcggctc tcttttctgg gcgtatctca tgacggtagg gcccttcagc aagggcggcg
     3961 gcgccgagtt gaccgcgttc ggccagagcg ccgtggctat cgcgcaagcc aagacgctgc
     4021 cctacatggc gaacggcggg gcgggtctgt ggtcggcctt catgaagggt atcggatgta
     4081 accttctcgt caaccttgcg atccttctgg cgctttcatc caagaacatg atcggcaagt
     4141 tcttcgggat atggttcccg atcatggcct tcgtttcgtc cggcttcgaa cactgcgtgg
     4201 ccaatatgta tttcatcccc gcaggtatca tgctgggcgc gaatgtcacg tgggggcaat
     4261 tcatcggatg gaatctcatc cccgtcacga tagggaacat cgtcggtggt tttgtgttca
     4321 tcggtgctgt atatttcatc tctttcaaga aagagctttc aaccatcaca ccaacctgat
     4381 acgaagcaat gcaccccgga gcgcgaacgc tccggggtgc attcaagacc gtctacccat
     4441 cgtatcaact gcagaggcgt gcttcggctg cctccaaggt ttccatcttc ttcgcgaaag
     4501 cgaagcgaac cgtcttcttg cccatgccct tcgtgccgta gaaacatgac ccggggacaa
     4561 cggcgactcc caggttcgcg ggaagccagc gggcgaagga gtagtcgtca ccgtcaaaat
     4621 cccaggcgct gaaatccgcc atggcgtaga acgctccctc gggcgagtgg gcggaaaaac
     4681 cggccctctg cagaatggat atcatcttgt cccttctgag cgtgtagtcc cggcggagcc
     4741 cggtgtagta ctcgtcgggc agtgcgagga cggcggcgca ggcagcctgc aatggtgccg
     4801 gggcgcagat ggtcatgaaa tcgtgaacgg tcctgaggat ggtgctcagc ggattgaggg
     4861 cgcaggcata gccgagccgc cacccggtca tggcgaaggt ctttcccagt ccgctgatcg
     4921 ttaccgtgcg ttcttccatg cccgggatgg tggcgatggg gacgtgaggc tgtccgtcgt
     4981 atatgatatg ctcgtagatc tcgtcggtga cggcgacgac attgaattcc cggcacagtt
     5041 cagcgatgcc ctccagttcc tccatggtga agatgtgccc cgtcgggttg tggggggtgt
     5101 tgatgagaat ggcgcgggtg cggtcgttga acgcgcgccg c
//
LOCUS       DAOPOA010000067         5138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17664_length_5138_cov_3.123156, whole genome shotgun sequence.
ACCESSION   DAOPOA010000067 DAOPOA010000000
VERSION     DAOPOA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17664_length_5138_cov_3.123156"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /gene="amrB"
                     /locus_tag="PKJ17_02345"
     CDS             complement(<1..473)
                     /gene="amrB"
                     /locus_tag="PKJ17_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOD74837.1"
                     /translation="MGDMRKTYANKGFAARILMAALAACVLVLAACEALAQSAAPVRK
                     PVFAGSFYPADKVSLQTMVDGYLKDAQRVGDALPGVFAVIAPHAGYVYSGKTAAYSYN
                     AARRKGITTVILLGSSHRSSFKGIALYPTGSWETPLGKVPVDSAMGRKMASLCEAV"
     gene            complement(461..1567)
                     /gene="amrS"
                     /locus_tag="PKJ17_02350"
     CDS             complement(461..1567)
                     /gene="amrS"
                     /locus_tag="PKJ17_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOD74838.1"
                     /translation="MRRRDFLKVAALTPFVLAAGTNVLGQAARPAFHEALYYRKKGSN
                     KVVECLLCPRRCTLPDGATGFCRARKNISGRLYSLGYGSPCAAHIDPVEKKPFFHVLP
                     RTYSFSVASAGCNFRCQFCQNWQISQASPLETANTTLSPQHVVAAAVRKDCRSIAYTY
                     TEPTNFYEYMFDTAKTARGRGILNVAHSNGYINPEPLKALCRYMDAVNIDLKGFSEDF
                     YGKLCEGELAPVLLTIKTLKRSGVWVEITNLVIEGYNDDEKMVREMCRWVVKEVGRDV
                     PVHFSRFYPMYKLTGVSPTSVRTLERSRDAGFEAGLQFAYIGNVPGHPGENTYCPTCR
                     TMLIQRSGYNVLNVTLKNGTCPSCGTKIGGIWAT"
     gene            complement(1624..3891)
                     /locus_tag="PKJ17_02355"
     CDS             complement(1624..3891)
                     /locus_tag="PKJ17_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74839.1"
                     /translation="MIASLISLLLVTGLSGIVAQTVLIRECLILYGGNELSIGVTIGA
                     WVVWEALGAFIGGRWPRGARGAAHAFIAAGLIFGIVFPASIYAIRAFKVIAGLPPEIS
                     LGITSIFGASMVIFLPAGLLHGFAFTAACRVYEGVKGPGGKAAGKVYFYEMAGTIAGG
                     ILVSYVLVTWLHSFRIAGLILVLMALACLAMAVSAATRAGKLLAALSLAIALGSAVFM
                     AAGLDGRLQLRSIAAQWHGREVVSYENSLYQNIAVTRATGQYTFFTDGLPAATIPVPD
                     IARAEEIVHIPLLTHGTPRNVLVLHGGAGGVIGEVLKYPTVRRVDYVEIDPAYLGTLV
                     RYPSALVRSELGDPRVILHYTDGRRFVKAASPDRRYDVVLLGVASPATLQANRFFTLE
                     FFREVRRILTDEGILVFTVPGSLAYYDRELRDINMSAVATAGEVFAHVAVVPGDQNIF
                     LASASARDIDLSPQRLVDRLNDLRLTTRLISLPHLTYRLDRERMEWFRRAVEPSRAEV
                     NRDLAPKGLYYTIAYINALHTPSMKGLFAAAGRRGIPVFLAALGLFAAGAFLLKNRHR
                     RIPALFVISTTGFVVMLLELSLIFVFQVAYGYVFHEIGMLLTMLMAGMAAGSMAAARR
                     VTTAGVSKAFMAAEASLASFCLLLFFLFTLTGHGAGAGGLSGRLLFFALLFVPGFFAG
                     MEFPLAVELFEDREGTGGSVGPVYAGDLAGGFIGGMLGGFFLFPLMGLAMSCLLFGAL
                     KAAGLLLLLLSGKRK"
     gene            4120..4545
                     /locus_tag="PKJ17_02360"
     CDS             4120..4545
                     /locus_tag="PKJ17_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74840.1"
                     /translation="MKRERVLLLAALPVLMAAVLAMISVTADAQVFSKEVTVGELVAN
                     PSQYDQKNVTVNAYAFIVRQKKDRGGNPFILVSIFDPRDRKKVITAFGPGSTQARNGD
                     MIKLTGKFKARSKRGRYTYDNEIETTGDKVVVTAKGTGP"
     gene            4623..>5138
                     /locus_tag="PKJ17_02365"
     CDS             4623..>5138
                     /locus_tag="PKJ17_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74841.1"
                     /translation="MGKKRFILTLIILVASLAAGLVLLARTPLLLRAVAAVSGPLFGY
                     DMAAESFSFYPLKAEVKGFTIADIRGKNFIFTSSRVSVSSSPGSAFRGNVEKVLLKDA
                     KIRIRLGDRRETETDLSFIRKIPPVHLLTMENGEFTLTFKGSPGTITLKNIDLTVKGF
                     SPDHGGALAFTG"
BASE COUNT         1087 a         1500 c         1625 g          926 t
ORIGIN      
        1 acggcctcgc agagggaggc catcttcctg cccatggcgg agtcgacggg cacctttccg
       61 aggggtgttt cccagctgcc tgtcgggtag agggctatgc ccttgaagga actgcgatgg
      121 ctcgagccga ggaggatgac cgtcgtgatg ccctttctcc tggcggcgtt gtatgaatag
      181 gccgcggtct ttcccgaata gacgtacccc gcgtgagggg cgataacggc gaacaccccc
      241 gggagcgcgt cgcccacgcg ctgtgcatcc ttcaggtacc cgtccaccat ggtttgcagg
      301 gacaccttgt ccgctgggta gaaggagccc gcgaagacag gctttctcac cggggcggcg
      361 gattgcgcga gcgcctcgca tgccgccagg acaagaacgc aggcggcaag cgccgccatc
      421 aggatccggg ccgcaaagcc tttgttcgca tacgtctttc tcatgtcgcc catattcctc
      481 ctatctttgt gccgcacgag gggcacgtgc cgttcttcag ggtgacgttg aggacattgt
      541 accccgatct ctggatgagc atcgtcctgc acgtcgggca gtaggtgttc tctccgggat
      601 ggcccgggac gttccctata taagcgaact ggagccctgc ttcgaagccg gcatcgcggg
      661 acctctccag cgttctcacc gatgtgggcg agacccccgt cagtttgtac atggggtaga
      721 agcgggagaa atggacgggc acgtcccgtc cgacctcctt cacgacccac cggcacatct
      781 ctctcaccat cttttcgtca tcgttgtagc cctctatgac gaggttcgtg atctccaccc
      841 acacgccgga ccgcttgagc gtctttatcg tgaggagaac gggcgcgagc tcgccttcgc
      901 agagcttgcc gtaaaagtcc tcggaaaatc ccttgaggtc gatattgacg gcgtccatgt
      961 acctgcaaag agccttgagg ggttcggggt tgatgtagcc gttggagtgc gcgacgttga
     1021 ggatgccccg cccgcgggct gtcttcgccg tgtcgaacat gtactcgtag aagttcgtcg
     1081 gttccgtata cgtataagcg atgctcctgc agtcctttcg gacggcggcc gcgacgacat
     1141 gctgcggcga cagcgttgtg ttggcggtct cgaggggaga tgcctgggag atctgccagt
     1201 tctgacagaa ctgacaccgg aagttgcagc cggcactcgc cacggagaag gaatacgttc
     1261 tgggaaggac gtggaagaac ggtttcttct ccacggggtc gatgtgcgcg gcgcagggcg
     1321 agccgtagcc gagggaatag agcctgccgg agatgttctt gcgggcccgg cagaaacccg
     1381 tggcaccgtc tggaagcgtg cagcggcggg ggcacaggag gcactcgaca accttgttcg
     1441 agcccttctt ccggtagtag agggcctcgt ggaaggccgg ccgggccgcc tgccccagaa
     1501 cattcgttcc tgcggcaagg acgaaaggcg taagtgctgc aaccttcagg aaatctcttc
     1561 ttctcaccgt ggcaccgtca ccgtggtata ctgcggaata ccgcccaatt ccttgctatt
     1621 atactacttt ctcttccccg ataacagaag aagaagcagc ccggcggcct tgagggcgcc
     1681 gaaaaggagg cagctcattg cgaggcccat gagaggaaag aggaagaagc ctcccagcat
     1741 gccccctatg aaacccccgg cgaggtcgcc cgcgtagaca ggccctacgg accccccggt
     1801 gccctctcta tcctcgaaga gctccacggc aagagggaat tccattccgg caaagaaccc
     1861 cggcacaaag aggagggcaa agaagagaag tctgcccgaa aggcctcccg cgcccgcgcc
     1921 atggcccgtg agagtgaaca ggaagaaaag gagaaggcag aaggatgcca gggaggcttc
     1981 cgccgccatg aaggccttcg atacccctgc cgtcgtgaca cgccgggccg cggccatgct
     2041 gccggcggcc atgccggcca tgagcatggt gaggagcatg ccgatctcgt ggaagacgta
     2101 cccgtatgcc acctggaaga caaagatgag ggacagttca aggagcatga cgacgaaacc
     2161 cgtcgtggag atgacaaaga gagcaggtat tctccggtgc ctgttcttca ggaggaacgc
     2221 cccggccgcg aaaaggccca gagcggcgag gaatacggga ataccccggc gcccggcggc
     2281 tgcgaaaaga cccttcattg aaggggtgtg aagggcgttt atgtatgcga tcgtatagta
     2341 gaggcccttc ggggcaaggt cacggttgac ctcggcccgg gagggctcga cggcgcggcg
     2401 gaaccattcc atacgctccc tgtcgagacg gtacgtgaga tgtggcagcg agatgagccg
     2461 ggtggtcagc cgcaggtcat tcagtctgtc taccagtctc tgcggtgata gatcgatgtc
     2521 tctagccgac gccgaggcga ggaagatgtt ctggtccccc ggaacgacgg caacgtgagc
     2581 gaagacctcg ccggcggtgg ccacggcgga catgttgatg tccctgagtt ccctgtcgta
     2641 gtaggcgagg gagccgggaa cggtgaatac aaggatgcct tcatcggtga ggatccgccg
     2701 cacttcacgg aagaactcca gggtaaagaa cctgttcgcc tgaagcgtcg cgggggaggc
     2761 aaccccgaga agaacgacgt cgtagcgccg gtcgggggat gccgccttca cgaagcgcct
     2821 gccgtctgtg tagtgcagga tgacccgcgg atcgccaagt tcggaccgga cgagtgcaga
     2881 gggatagcgg acgagcgtcc cgagataggc ggggtcgatc tctacgtaat cgacacggcg
     2941 caccgtgggg tacttgagga cctcccctat gacgccgccc gctccgccgt gaaggacaag
     3001 gacgttccgc ggggtgccgt gggtgagaag cgggatgtgg acgatctcct ccgcacgggc
     3061 tatgtcgggc acgggtatgg tggccgccgg caggccgtcg gtgaagaacg tgtactgccc
     3121 cgttgccctc gtcacggcaa tgttctgata gagggaattt tcgtaggaga cgacctcgcg
     3181 accgtgccat tgcgcggcga tggaccgcag ctgcaggcgt ccgtcaaggc ctgcggccat
     3241 gaagacggcc gagccgagcg cgatggcaag gctcaaagcc gcaaggagct tgcccgcgcg
     3301 ggttgccgcc gagacggcca tggcaaggca ggcgagggcc atgagaacga gtatcaggcc
     3361 cgcgatccgg aacgaatgga gccaggtgac aaggacgtag cttaccagta tccccccggc
     3421 gatggtaccg gccatctcgt agaaatagac ctttcccgcc gccttgcccc cggggccctt
     3481 cacaccttca tagaccctgc aggccgccgt gaacgcgaaa ccgtggagaa ggcccgcggg
     3541 gagaaatatc accatggacg cgccgaagat gctcgtgatg ccgagggata tctccggagg
     3601 gagacccgcg atgaccttga aggcgcggat ggcatagatg cttgccggga agacgatgcc
     3661 gaagatgagg ccggcggcga tgaacgcatg cgccgccccg cgggccccgc gaggccaccg
     3721 cccgccaata aaggcgccaa gggcctccca gacgacccag gcgccgatgg tcacgccgat
     3781 ggaaagctcg tttcccccgt aaaggatgag gcactccctg ataaggaccg tctgcgccac
     3841 gatccccgag agtccggtca cgagaagaag agatatgaga gatgcgatca tgcgtggacc
     3901 ttgagaagac gggttatagg taatgggtta tagataatgg gaaagacctt tctttgatcc
     3961 atacccatca tctatgacct atcacccata acccgtctct tctgtttcct ttacatacca
     4021 tagcggaagg aggggagaaa ggcaagaagc caggttttat tgactcttcc ccggaaacgg
     4081 gatataggtt aaaaaacgag atcgaagggg gaacagatag tgaaaagaga aagagtcttg
     4141 ttgctggccg cactcccggt cttgatggcg gcggtcctcg cgatgatctc cgtgacggcg
     4201 gacgcccagg tcttctccaa agaggtaacc gtgggcgaac ttgtcgcgaa tccttcccag
     4261 tacgatcaga agaatgtcac cgtcaacgcc tatgcattca tcgttcgtca gaagaaggac
     4321 cgcggcggga acccgttcat actcgtcagc attttcgacc ccagggacag gaagaaggtc
     4381 ataaccgcct ttggcccggg aagcacccag gcccgcaacg gggatatgat caagcttacg
     4441 ggcaagttca aagccaggtc gaagcgcgga cgctacacct acgacaacga gatcgagacg
     4501 acgggcgaca aggtggtcgt gacggcaaag ggcaccggcc cataatgacc gcagcatgac
     4561 cggaagtacg ctgaccgcga cttctcgcgg gccttcgtgc tataatgccg gcggaacgag
     4621 cgatgggaaa aaaaaggttc attctcactc ttattatcct tgttgcctct ctcgcggcgg
     4681 ggctggtcct ccttgccagg acgccgcttc tcctgcgcgc tgtcgccgcg gtgagcgggc
     4741 ctcttttcgg ttacgacatg gcggcggaat ccttttcgtt ctatcccctg aaggcggagg
     4801 tcaaggggtt cacaatcgcc gacattcgcg gaaagaactt catcttcacg agttcccggg
     4861 tcagcgtctc ttccagtccg gggtccgcct tccggggcaa cgtagagaag gtccttctca
     4921 aggacgcgaa gatacgcatc cgtctcggcg acaggagaga gaccgaaacg gacctgtcct
     4981 tcatccggaa gatacccccc gtccaccttc tgaccatgga gaacggggaa tttaccctca
     5041 ccttcaaggg ttctcccggt accatcaccc tcaagaatat cgacctcacc gtgaagggtt
     5101 tctcgccgga ccatgggggc gcgcttgcct tcaccggc
//
LOCUS       DAOPOA010000068         5121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17743_length_5121_cov_3.410186, whole genome shotgun sequence.
ACCESSION   DAOPOA010000068 DAOPOA010000000
VERSION     DAOPOA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17743_length_5121_cov_3.410186"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKJ17_02370"
     CDS             <1..462
                     /locus_tag="PKJ17_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74842.1"
                     /translation="VLRAARETDGKMTALTAGGKEKTFALHGELARTMSTNVGIFRER
                     AEMEKALEEIGRIGERYRDIGVSSPDRHMNYELMNAVELEYMIEVAHTIAHGALLREE
                     SRGAHSRRDFPKRDDETWLRHTIARMNGDGSVGISFRDVTITRYQPMERVY"
     gene            459..1199
                     /locus_tag="PKJ17_02375"
     CDS             459..1199
                     /locus_tag="PKJ17_02375"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOD74843.1"
                     /translation="MITTTAYTFKILRSDRKRPDERPWFQTYRVKVIPGLTVLAVLNR
                     IRDEIDGTLSFRSSCRSAVCGSCAMVINGRIDLACRTQVASFGANTIILEPLPNMEII
                     KDLVVDMTPFWRMYEKVRPWLVRRTPDPGKEIEQSEEERSRIDQFVNCILCACCYGAC
                     PVIARDPEYLGPAALAKLERFILDSRDERPDDFLDTVNDEKGVWGCDTVLRCIEACPK
                     DVRPTDSIVGLRKALVKKKARQLLGMKK"
     gene            1196..1666
                     /locus_tag="PKJ17_02380"
     CDS             1196..1666
                     /locus_tag="PKJ17_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HOD74844.1"
                     /translation="MKLDHSAVTRRTFLNTLLTGWAAAVASGTLWALIKFAFPTLGKE
                     PDFVVLKRADFEGIPLNSVKPFAWGGKLGLYFKKQNGTVEALKGVCTHMECNITYKPD
                     EKKFYCACHKGWFDENGRNIEGPPPKPLEIFDIIVEGDKLIIAKKGVKVELPKA"
     gene            1692..2711
                     /locus_tag="PKJ17_02385"
     CDS             1692..2711
                     /locus_tag="PKJ17_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HOD74845.1"
                     /translation="METLGALARSKTVPSHRFDVWYFAGGLTLFLFVFQFITGMALAL
                     YYVPHAEHAHKSIVDIVTKLNMGWLFRSLHHWGAQLAILVLFAHVFSTLLLKAYRKPR
                     ELVWLTGFVMLAISIFFGLSGYFLLWDERAFAAVRVATGGAGNLPLVGWVIKGFLRGG
                     LDVTGETLTRFYAFHVAILPLVTIAVAGLHILLVQIHGMSVPLSQERKQGGPEPFFPN
                     LFYKDLIVWLVCLGVVVTLAVLLPPEIGRKADPLAPAPENIKPEWYFLFLFQTLKLFP
                     GEMLGMNGETIAIVIISAAILFFFLIPFFDRRSAMGQRSPLFTWIGTFYLVYFTVMTV
                     IGFLT"
     gene            2723..2977
                     /locus_tag="PKJ17_02390"
     CDS             2723..2977
                     /locus_tag="PKJ17_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74846.1"
                     /translation="MLRVFLIVLFFLIIGTTALCAGAEPKVEVFPPASYDRHIIYQAL
                     IFFWIGIIGLIVIIAMKLREIKRVQKMGIDEEEGNVPFLD"
     gene            3013..4905
                     /locus_tag="PKJ17_02395"
     CDS             3013..4905
                     /locus_tag="PKJ17_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74847.1"
                     /translation="MQTIVRNSLISRRGLQQILSLPEEDVVYVSLLEILAKFQDIKQF
                     ASEIHTEIHLIKPTLKILGYSYESKPKYFNDSIKGPDVALFATEADRDRTSPLWGTPE
                     YYMNTLGVLLLKRFGRNLEEGVSGFYLEFENRIPSYQLFYFLKNTKTPWGILTNGKQW
                     MLMKKPLAFETRVFSVDLEEAIETNDRDALHLFCKIFSVNGLSTVVPELQEAERQSLV
                     DRLTDKKASLRNTTAGFRKKTEVFPRIVGGLSDLFAEDIFTSTRAYLTENDVYVAKRT
                     TPPNTIDEFNIADITSYLLNKKGSSPVIDPERIFLHARPEELTKDDLLAMRVLDMTPG
                     FGNVTTQLVDGIAYLSFILPYRDRNTFVAQWENEKALKRYILQRILYGIEKSHVAYDI
                     LQYAMQHRYGTEADNYRFGNPLIGMSLSDIAPHVDTRNQMGLFSKNPLDILKDVSKMY
                     RQYFSLSDKIKEDMAVKEELASQLNVYCQRIRDVMDLITATYFSKAIDERKIHESLLM
                     LDSDNASWDSHVSRDWFAEAKRIARRNGFFHLEIEFPFLVDGAFDYIFVQPSLTHIWE
                     DPFPLPEVTKAHIKRGMTYLKPEGTMVLILDRPDEGLLTELSRSKRYNTRAEENIILL
                     RKKKMA"
BASE COUNT         1148 a         1590 c         1363 g         1020 t
ORIGIN      
        1 gtccttcgcg cggcccggga aacggacggg aagatgaccg cgctgactgc gggaggaaag
       61 gagaaaacct tcgcccttca cggcgagctc gccagaacga tgagcacgaa tgtggggatc
      121 ttccgtgaac gggcggagat ggaaaaggcc ctcgaagaga tcggccgcat cggggagcgt
      181 taccgggaca tcggcgtttc gtctcccgac aggcacatga actacgagct catgaacgcc
      241 gtcgagcttg aatatatgat cgaggtggcc cacacgatag cccacggggc acttctgagg
      301 gaagagagcc gcggggccca ttccaggagg gacttcccga aacgcgacga cgaaacgtgg
      361 ctgaggcaca ccatagcccg catgaacggc gacgggagcg tcggcatctc cttccgcgat
      421 gtcaccatca cgcgctatca gcccatggag agggtctatt gataacgacg acggcatata
      481 ccttcaagat actgcgcagc gacaggaaaa ggccggacga aaggccctgg ttccagacgt
      541 accgcgtgaa ggtcatcccc ggcctcacgg tccttgccgt tctcaacagg atccgcgacg
      601 agatcgacgg caccctgtcc ttccgttcgt cctgcaggtc cgccgtgtgc ggctcctgcg
      661 ccatggtcat caacggcagg atcgacctcg cctgcaggac acaggtggcc tcattcggcg
      721 cgaacaccat catcctcgaa ccccttccca acatggagat cataaaagac ctcgtcgtcg
      781 acatgacgcc tttctggcgc atgtacgaga aggtgaggcc ctggctcgtg cggcggacgc
      841 cggaccccgg gaaagagata gagcagagtg aggaggaaag aagccgcatc gaccagttcg
      901 tcaactgtat cctctgtgcc tgctgctacg gggcctgccc cgtcatagcc cgcgatcccg
      961 aatacctggg acccgcggcc ctggcaaagc tggaacgctt catactcgat tcgcgggacg
     1021 agcggcccga tgactttctc gataccgtga acgatgaaaa gggcgtgtgg ggatgcgaca
     1081 ccgtgcttcg gtgcatagag gcctgcccga aagacgtgcg tcccaccgac tccatcgtgg
     1141 gcctcaggaa ggccctcgta aagaagaagg caagacaact cctggggatg aagaaatgaa
     1201 gctcgaccat tcagcggtga cgcgaaggac atttctcaac accctcctca ccggatgggc
     1261 cgctgccgtc gcctccggga ccctctgggc cctcatcaag ttcgcctttc cgaccctcgg
     1321 gaaagagcct gacttcgtcg tcctcaaaag ggccgacttt gagggcatcc ccctcaactc
     1381 cgtgaagccc ttcgcgtggg gaggcaaact gggcctctac ttcaagaaac agaatggcac
     1441 cgtcgaggcc ctcaagggcg tctgcaccca tatggaatgc aacatcacct acaagcccga
     1501 tgagaaaaag ttctactgcg cctgccacaa ggggtggttc gacgagaacg gcaggaacat
     1561 agaggggcct ccgccgaagc ccctcgagat cttcgacatc atcgtcgaag gcgacaagct
     1621 catcatagcg aaaaagggag taaaggttga gctgccgaag gcttaaagac tggttcgggg
     1681 aacgatacga catggagacg ctgggggccc tcgcccgttc caagaccgtg ccgtcccacc
     1741 ggttcgacgt ctggtatttc gcaggggggc tcaccctctt ccttttcgtc ttccagttca
     1801 taacggggat ggcgctcgcc ctctattacg tcccccatgc ggagcacgcc cacaagagta
     1861 ttgtcgacat cgtcaccaag ctcaacatgg gatggctttt ccgctccctc caccactggg
     1921 gcgcgcagct cgcgatactg gtcctcttcg cccatgtctt cagcaccctc cttctcaaag
     1981 cctaccggaa accgcgggag ctcgtctggc tcaccggttt cgtgatgctt gccatatcga
     2041 tcttcttcgg actcagcggg tattttctcc tctgggacga gagggccttc gccgcggtgc
     2101 gcgtggcgac aggcggggcc ggcaatcttc cccttgtcgg ctgggtgata aagggtttcc
     2161 tgcgcggcgg cctcgacgtg acgggcgaga cgctgacacg cttctacgca ttccacgtcg
     2221 ccatcctgcc gctcgtcacg atcgcggtgg cggggctcca catcctcctc gtccagatcc
     2281 acggtatgag cgtacctctc tcacaggaaa ggaaacaggg aggacccgag cctttcttcc
     2341 ccaacctctt ctacaaggac ctcatcgtat ggctggtctg cctcggcgtc gtcgtcaccc
     2401 tggccgtcct ccttccgccg gagatcggca ggaaggcaga cccgctcgcg cctgcgccgg
     2461 agaacatcaa gcccgaatgg tacttcctct ttctcttcca gaccctcaag ctctttcccg
     2521 gcgagatgct gggcatgaac ggcgagacca tcgccatcgt catcatctcc gcggccatac
     2581 tctttttctt cctcatcccc ttcttcgaca ggaggtcggc gatggggcag aggagccctc
     2641 tcttcacgtg gatcggtacg ttctatcttg tctatttcac ggtcatgacc gtcatcggtt
     2701 ttctcacata ggggtgacag cgatgctcag ggtgtttctc atcgtccttt tctttctcat
     2761 tatcgggaca acggcgctct gcgccggggc cgagccgaag gtcgaggtct tccccccggc
     2821 gtcctacgac aggcacatca tctaccaggc gcttatcttc ttctggatag gcatcatcgg
     2881 cctcatcgtc atcatcgcga tgaagctgag ggagataaaa cgcgttcaga agatgggcat
     2941 cgacgaagag gaagggaacg ttcctttcct tgactaagca ggcccaaacc tttattatgt
     3001 agcatcaatc ctatgcaaac gattgttcgt aactcgctta tatcccgccg ggggctccaa
     3061 caaatcctca gccttcccga ggaggatgtc gtctacgtct cccttctcga gatactcgcg
     3121 aagttccagg acataaagca gttcgcaagc gagatacaca ccgagatcca tctgatcaaa
     3181 ccgaccctca agatactcgg ctactcctat gaatcgaagc cgaagtactt caatgattcc
     3241 atcaaaggcc ccgatgtcgc cctcttcgcg acggaagccg accgggacag gacgtctccc
     3301 ctgtggggga cgccggaata ttacatgaac acgctgggcg tccttcttct gaagcgcttc
     3361 gggagaaatc tcgaagaggg tgtcagcggt ttctatctcg agttcgagaa ccggattccc
     3421 tcgtaccagc tcttctattt cctgaagaac acgaagaccc cctggggcat actgaccaac
     3481 ggcaaacagt ggatgctcat gaagaaacct ctcgccttcg agacgcgcgt gttctccgtg
     3541 gacctcgagg aggccatcga gacgaacgac agggacgcgc tccatctctt ctgcaagatc
     3601 ttctccgtca acgggctctc gacggtggtc cccgaactcc aggaagccga gcggcaatcc
     3661 ctcgtcgaca ggcttacaga taagaaggcg tccctgcgca acacaacggc gggcttcagg
     3721 aagaaaacgg aggtctttcc caggatcgtg ggcggtcttt ccgacctttt cgccgaggat
     3781 atctttacct cgacccgggc atacctgact gagaacgacg tctacgtcgc aaagaggact
     3841 acacctccca acaccatcga cgaattcaac atcgccgaca taacatccta ccttctcaac
     3901 aagaaaggtt cctctcccgt catcgatccc gaaaggatat tcctccacgc aaggccggag
     3961 gaattgacaa aagacgacct cctcgcgatg agagtgctcg acatgacgcc cggcttcggc
     4021 aacgtcacga cgcagctcgt ggacggcatc gcctatctct ccttcatcct tccctatcgc
     4081 gacaggaaca ccttcgtggc gcagtgggag aatgaaaagg ccctcaagcg gtatatcctt
     4141 cagaggatcc tctatggtat cgagaagtcc cacgtcgcct acgatatcct ccagtatgcc
     4201 atgcaacacc gttacggaac ggaagccgac aactacaggt tcggcaatcc tctcatcgga
     4261 atgtcgctct ccgacattgc cccccacgtg gatacgcgca accagatggg gctcttctcg
     4321 aagaaccctc tcgacatcct caaggacgtc agtaagatgt accggcagta tttctccctt
     4381 tccgacaaga taaaggaaga catggcggta aaagaggagc ttgcttcaca gctcaacgtt
     4441 tactgccaac ggatacggga cgtcatggat ctcataacgg caacctactt cagcaaggcc
     4501 atcgacgaaa gaaagataca cgaatcactc ctcatgctcg attccgacaa tgcctcctgg
     4561 gactcccacg tgtcccggga ctggttcgcg gaggcgaaac gcatcgccag gcgaaacgga
     4621 ttcttccatc tcgagatcga gttccccttc ctcgtcgacg gcgccttcga ttacatcttc
     4681 gtccaaccct ccctcaccca catatgggaa gaccccttcc ccctccccga ggtcacgaag
     4741 gcccacatca agaggggaat gacctacctc aaacccgaag gcacgatggt cctcatcctc
     4801 gaccgacccg atgaaggcct cctcaccgaa ctctcccgct ccaaacgcta caacacccgc
     4861 gcggaagaga acatcatcct cctcaggaag aaaaagatgg cttgaaccgt ttgagccgct
     4921 tgagggttga agacaagaga catcccttcc tgtgtccgag agggtggagg gatcgtgttc
     4981 gcaccggtac acagatacga gcagcagtat ttctctattc tgtaacgttc aagcggttca
     5041 agccgtcttt agaataccag cgttttgagc aggcggtagg atctgaagtc cacgtctatg
     5101 tggtaggcga ggaagctttc c
//
LOCUS       DAOPOA010000069         5115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17771_length_5115_cov_3.374506, whole genome shotgun sequence.
ACCESSION   DAOPOA010000069 DAOPOA010000000
VERSION     DAOPOA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17771_length_5115_cov_3.374506"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PKJ17_02400"
     CDS             complement(<1..289)
                     /locus_tag="PKJ17_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD74848.1"
                     /translation="MREASDRGAGTLNETDGGRLFALTGATGFLGSHLMAGLIRAGHR
                     VVVLGRSRNGTGLADRVDALLDWFGLESCRERIDAVEVDLLEPRLGLSRRRY"
     gene            complement(282..2114)
                     /locus_tag="PKJ17_02405"
     CDS             complement(282..2114)
                     /locus_tag="PKJ17_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOD74849.1"
                     /translation="MDEALRIAVNCRHYAMCKIDFLGTGVCPSGLAHHYVSFYPEGRM
                     DLYAALAKHRVPVTEECVRIVESCDLCGRCDKQCCFITGLRPTRVMEALRSLVAAHLA
                     AGRPVERPAGDLLLARIRDIVGERWATNDPAIRATYAHDPSPLALFRMPRYVILPGTR
                     EEVSSVVRLLRDAGVPWAARGNATNLMGFGFSEGAVLDLNRMKEMEFDEKNWCVKIGP
                     GVTAFDLQREAAGRGYRVNVAEPAALVCASMMCAGIVSLFSTAYGASADSHVDAEFVA
                     TDGSFFSLNEKNAPNLYAFDRAGAESPGICCSLKVKLHPVTTDEGGVLVPFDSLEKAI
                     DFSRECAVRRIGIAIGVLGTEYLSSFMSPSVRLALEIRDVLTGKLGMACAVLVLGDAY
                     ALGSIRAMGHPLIDQRLFTTLSLGLPSLAGAQWLDLVAALGEDEPFSYLKIGGFADLA
                     ETALCPSPSRLVQDFDPDLRQVYEEAYSRPDLTDLVWLNMFRITSSRVGREKHFLPFL
                     MYLPLDHGLVSEVSGGLQAIAGAKGLKNALGFITPVDSGKRCILEYDYFLDQNDEGEI
                     ERMRQAAAEAGALIDDLSDRTGTIRWMRYIFQQGYCRKENLLYA"
     gene            complement(2354..3064)
                     /locus_tag="PKJ17_02410"
     CDS             complement(2354..3064)
                     /locus_tag="PKJ17_02410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOD74850.1"
                     /translation="MAADDKKSLIVLREKHQVDQYIREITGQSMPVRVVIDDTIVLDD
                     CRLVMREGKRRDLFVEASLAPGEYPEAGRATITCAGDSSLYTFGTKVLSIELAEHRRM
                     HLTIQYPDRITKRERRRHVRVRPSANRPVSARIVIDNDEVVQVEPVDISTGGISFMMT
                     EEMSRFKSGDAVELVITVPAFGEVRAGATVRSVVHLMDLTRIGVEFSSLPDDALRLIT
                     EYVSERERQTGLAVFEDN"
     gene            3187..3729
                     /locus_tag="PKJ17_02415"
     CDS             3187..3729
                     /locus_tag="PKJ17_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HOD74851.1"
                     /translation="MMNRALILFLLLAAILFPPPGAGCAEYGKDVTVSLCFTPGGSCT
                     DRIIREISSARSEILIQAYSFTSAPIAKALIEAHKRGVKVIAILDKSQRTAKYTSATF
                     LANMRIPTYIDSRHAIAHNKVIIIDGAVTITGSFNFTKAAQEKNAENLLIIRSREIAR
                     AYRENWQHHLSHSERYEPKY"
     gene            3869..>5115
                     /locus_tag="PKJ17_02420"
     CDS             3869..>5115
                     /locus_tag="PKJ17_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD74852.1"
                     /translation="MITVFIDDKECSAREGSTIIDAARENGIDIPSLGYDPRVSPPTN
                     IEISMVEVVENGRPRTVSATSTVMEDGMVITTRSPALESFRKIYLQALLRNHYGDCVA
                     PCVARCPASIDIQKYLYHVANGNFVEALAVIREVNPFPSVCGRVCPHPCEAQCRRNAV
                     DSPVNINAVKRFVGDFDSYQTVEFTPFVAPDTGRRVAVIGAGPAGLTCAYYLRCLGHR
                     VTIFEKQEAAGGMLRWGIPYYRLPENILDREIRTILNLGIDIRYNKELGKDFTLLSLK
                     RDGYEAIFLGIGAQKTSRMAIPGEDLPGVMTGLDLLARLARYETPDLGDTVIVFGGGN
                     TAMDAARSAIRLGSKRVIVAYRRTRAEMPAEEAEIEEALEEGVEMMFLTAPLSINGEN
                     GSLSLRCMKMELGEPDGSGRRRPV"
BASE COUNT         1051 a         1667 c         1426 g          971 t
ORIGIN      
        1 catagcggcg gcggctgaga ccgagacggg gctcgagaag gtccacctcg acggcatcga
       61 tacgctcccg gcagctttca agaccgaacc agtcgagcaa tgcgtctacc ctgtcggcaa
      121 gacctgtccc gttccgggaa cgcccgagga ccacaacacg gtgacccgcc ctgatgaggc
      181 cggccatgag gtggctcccc agaaaccctg tcgctcccgt gagtgcgaac aggcggcccc
      241 catccgtctc attcaatgtc ccggctcccc ggtcagaggc ttcacgcata gagcaggttc
      301 tcctttctgc agtagccctg ctgaaagata taccgcatcc agcgtatcgt gcccgtgcgg
      361 tccgaaaggt cgtctatcaa cgcgccggcc tcggccgcag cctgccgcat ccgttctatt
      421 tcgccctcgt cgttctggtc gagaaaatag tcgtactcga gaatgcagcg ctttccactg
      481 tccacgggtg tgatgaagcc gagggcgttc ttcaggccct tggcaccggc tatggcctga
      541 aggccgcccg atacctccga caccagacca tggtcgaggg gaagatacat gagaaagggg
      601 aggaaatgct tctcccgccc caccctcgag ctcgtgatac ggaacatatt gagccacacg
      661 aggtccgtga ggtccgggcg tgagtaggct tcctcataga cctgcctcag gtcgggatcg
      721 aaatcctgta ccagccgcga cggggacgga cagagcgccg tctccgcgag gtcggcaaaa
      781 ccgccgatct tgagatacga aaagggttca tcttccccga gggcggcgac aaggtccagc
      841 cactgcgccc cggcaagcga gggaaggccg aggctgaggg tggtgaaaag ccgctggtcg
      901 atgagcgggt gacccatggc ccggatgctc ccaagcgcgt aggcatcgcc aagcacgaga
      961 acggcgcagg ccattcccag cttccccgtc agaacatccc tgatctcaag ggcgagccgg
     1021 accgagggcg acatgaaaga ggaaagatac tctgttccga gcacccctat ggctataccg
     1081 atcctccgga ccgcgcactc cctcgaaaag tctatggctt tctcaagaga atcgaaaggg
     1141 acaaggactc cgccctcatc ggttgtcacg ggatggagct tcaccttcag ggaacagcat
     1201 attcccggac tttccgcgcc agcccggtca aaagcataga ggttcggcgc gttcttctcg
     1261 ttgagagaga aaaaggagcc gtccgtggcc acgaactcgg catctacgtg gctgtcggca
     1321 gaggcgccat aggccgtgga gaacagcgac actatgccgg cgcacatcat gctcgcgcat
     1381 accagggccg cgggctccgc gacattcacg cgataccccc ggcccgctgc ctccctctgg
     1441 aggtcgaagg cagttacccc cgggcctatc ttcacacacc agttcttctc atcgaactcc
     1501 atctctttca tccggttgag atccagcacc gccccttcac tgaacccgaa ccccatgaga
     1561 ttggtcgcgt tgccgcgcgc cgcccaggga accccggcgt cacgaagcag cctcaccacc
     1621 gacgacactt cttctctcgt gcccggtagg ataacgtatc gcggcatcct gaagagggcc
     1681 agcggtgacg ggtcatgggc ataggtggcc ctgatcgctg gatcgttcgt cgcccaccgc
     1741 tctcccacga tgtctcgtat gcgtgccagc aggagatccc cggcaggtct ttcaactggc
     1801 ctgcctgccg cgagatgggc cgccacaagc gatctcagcg cttccataac cctggtaggg
     1861 cgcaggcctg tgatgaaaca gcattgctta tcgcacctgc cgcagagatc acagctctcg
     1921 acgatacgca cgcactcttc ggtgacggga acacggtgct tcgccagcgc cgcgtagagg
     1981 tccatccttc cctcgggata gaagctcacg tagtggtggg caagccccga cggacacact
     2041 ccggtgccaa ggaaatcgat cttgcacatg gcataatgcc tgcagttgac ggcgatgcga
     2101 agcgcttcgt ccatgatgcc ctccactccg taaggacggt cgtgcccggc agccgcgtca
     2161 tcaggctgat gccggctccg gagaaccgtt ggtacgtccg gcctgagcag gaccctcaat
     2221 aatgccttgg ttaggattta tagccgatac cgcggcgagt caagtgcgcc ccttcatcgg
     2281 gcggcaccgg ttaacgtttg gcgagggaga agcggtcttc cgccaggagc tcctttttga
     2341 ggaagctgcc tcttcagttg tcctcgaaca ccgcgaggcc cgtctggcgt tcccgctcgg
     2401 acacgtattc cgttatgagc cttaaggcat cgtcgggcag ggaggagaac tcgaccccga
     2461 tcctggtcag gtccatgaga tggacgacgc tcctcaccgt ggcccctgcg cgcacctctc
     2521 cgaacgcggg aaccgtgatg acaagctcga cggcgtcgcc cgacttgaaa cggctcatct
     2581 cttccgtcat catgaaggat atgccaccgg tacttatgtc taccggttcc acctggacga
     2641 cctcatcgtt gtcgatgacg atccgtgcag acaccgggcg gtttgccgac ggacggaccc
     2701 tcacgtgcct gcgtctttcc cgctttgtga tcctgtcagg gtactgaatg gtcaggtgca
     2761 tgcggcgatg ttcggcaagc tctatggaaa ggaccttcgt cccgaaggta taaagggagc
     2821 tgtcgcccgc gcaggtaatg gtggcccgtc ccgcctccgg gtattcccct ggcgccagcg
     2881 atgcctcgac aaagagatct ctcctcttcc cttcccgcat cacaagccgg caatcatcaa
     2941 ggacgatggt gtcatcgatg acgaccctga caggcatgct ctgcccggtt atctcccgga
     3001 tgtactggtc tacctgatgt ttctcgcgaa gaacgatgag actctttttg tcgtcagctg
     3061 ccatgatatc acacggtctc gatctttatc ctgttaattc tctttcaatt gctccccgtt
     3121 tacaagagaa aagtgcgatt gacttttccc gtgccttcat ctacgctatg tgcgaaacca
     3181 gcgctcatga tgaacagggc cctcattctc ttccttctcc tcgccgccat tttgtttccg
     3241 cccccaggcg ccgggtgcgc ggagtacggc aaggacgtca ccgtcagtct ctgcttcacc
     3301 cccggcggtt cctgcacgga caggatcata cgggagatct cctctgcgcg ctcagagatc
     3361 ctcatccagg cgtactcctt cacctccgcc cccatcgcga aggccctcat cgaggcgcac
     3421 aagcgcggcg tcaaggtcat cgcgatcctc gacaagagcc agcgcacggc aaaatacacc
     3481 tccgccacat tcctggcgaa catgaggata cccacataca tcgactcccg gcacgccatc
     3541 gcccacaaca aggtcatcat catagacggc gcggtaacga taacgggctc cttcaatttc
     3601 acgaaggccg cccaggaaaa gaacgccgaa aacctcctca tcatccgttc ccgggagatc
     3661 gccagggcct accgcgaaaa ctggcaacac cacctcagcc actccgagcg ctacgaaccc
     3721 aaatactaac ccccctatcc gtcctatccg tcctaagtgt cctataggtc ccataggtcc
     3781 tatcccctcc tatccgtcct attttccttt ttcccccact tactgtataa tgttgagctt
     3841 ttgcacactt accgcaacgg aggacgcagt gataacggta ttcatagatg acaaggagtg
     3901 ttccgcgagg gagggcagca ccatcatcga cgcggcccgg gagaacggaa tagacatacc
     3961 cagcctcggg tacgatcccc gcgtaagccc accgaccaac attgagatat ccatggtgga
     4021 ggtggtggag aacggaaggc cccgcaccgt ctcggcgaca tccaccgtca tggaggacgg
     4081 gatggtcatc accacccgct cgcccgccct cgaaagcttc aggaagatat accttcaggc
     4141 cctcctgagg aaccactacg gggactgcgt cgctccctgc gtcgcacgat gccccgcgag
     4201 catcgatatc cagaaatatc tttaccatgt ggccaacgga aattttgtgg aagccctcgc
     4261 cgtcatcagg gaggttaacc ccttcccctc ggtgtgcggc agggtctgtc cgcacccctg
     4321 cgaagcccag tgtcgcagga acgccgtgga ttcacccgtc aacatcaacg cggtaaagcg
     4381 cttcgtcggc gatttcgaca gctaccagac cgtggagttc acgcccttcg tggccccgga
     4441 cacggggcgc cgcgtcgctg tcatcggcgc cggcccggcc gggctcacct gtgcctacta
     4501 tctgcgctgc ctcggacaca gggtcacaat cttcgagaaa caggaggccg ccggcggtat
     4561 gctccgttgg ggcatcccct actaccgtct tccggagaac atcctcgaca gggagatcag
     4621 gaccatcctc aaccttggca tcgatatacg gtacaacaag gaactgggaa aggacttcac
     4681 cctcttgtcc ctgaaacggg acgggtacga ggcgatattc ctcgggatcg gcgcgcaaaa
     4741 gacctcccgc atggcgatcc ccggggagga ccttcccggt gtcatgacgg gcctcgacct
     4801 ccttgcccgc ctggcgaggt acgagacgcc cgacctcggc gacaccgtca tcgtcttcgg
     4861 aggggggaac acggccatgg acgccgcccg cagtgccatt cgcctgggat cgaagagggt
     4921 gatcgtcgcc tatcggagga caagggccga gatgcccgcc gaagaggcgg aaatagagga
     4981 ggccctcgaa gaaggtgtgg aaatgatgtt cctcaccgcc cccctgtcca tcaacggcga
     5041 gaacggctct ctgtccttac gatgcatgaa gatggaactc ggtgagcccg acgggagcgg
     5101 caggcgccgg cctgt
//
LOCUS       DAOPOA010000070         5108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17802_length_5108_cov_3.678805, whole genome shotgun sequence.
ACCESSION   DAOPOA010000070 DAOPOA010000000
VERSION     DAOPOA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17802_length_5108_cov_3.678805"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKJ17_02425"
     CDS             complement(<1..444)
                     /locus_tag="PKJ17_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74853.1"
                     /translation="MSHSRTILLVEVEAETAGARRRALERCGYTVIIVASNGEALERV
                     RSDPGIDLVIAGVDPGDGMNGPEAAEAILAERDIPVLFYFHPRSFRQNTTDRTGGPVC
                     YGYALAGFGEARLQVPVQAALELHRAHRRMKDPDMAESGGGDRERY"
     gene            complement(631..1971)
                     /gene="nspC"
                     /locus_tag="PKJ17_02430"
     CDS             complement(631..1971)
                     /gene="nspC"
                     /locus_tag="PKJ17_02430"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR01047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOD74854.1"
                     /translation="MGAGLIIEEALKYISAKDARAAAMYHALNDRNRRRVLGALDGKG
                     LKSLTRLVMKARSKVFREAEERTPGVAEGIVRTPAYLIDESLIEENMRVLRYVKDRTG
                     CRILHALKAYASFATFGVMTPYLDGVCASGANEARLGREEFGKEVHTFGAAYTEKDVR
                     AVLRYSDCVIFNSFHQLGRYGEAARRLGRQIGLRVNPGHAEVQTAMYNPCAPRSRLGV
                     VHDEFERRFPAVAGQVQGLHFHAMCEQNSDVLQRILKSFERLYGRYLKGLRWVNFGGG
                     HHITRDDYDLERLIGIIDAFKARYGVEVYLEPGEASVYHAGVLVSTVVDIVRNEMEIA
                     ILDTSAETHMPDVLLMPYRPHVMGSAEAGVKKHTYRLAGPSCLAGDVVGDYSFDERLP
                     RGARLLFTDMALYSIVKSTTFNGIGLPDIAVLRKDGRVEVVRRFGYGDYRNRLS"
     gene            complement(1979..3214)
                     /locus_tag="PKJ17_02435"
     CDS             complement(1979..3214)
                     /locus_tag="PKJ17_02435"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOD74855.1"
                     /translation="MNNKDHGRIMIIGAGGVATVAAHKCAQVPEVFGEILIASRTLRK
                     CDAIRDDIRLRYGRDIGTAKVDADNVPELVALIRSFGPDVVLNLALPYQDLHIMDACL
                     ETGVHYIDTANYEPLDEAHFEYSWQWAYQDRFREKGIMALLGSGFDPGVTNVFAAYAR
                     KHLFDEIHYLDILDCNAGTHGHVFATNFNPEINIREVTQVVRHWRQGEWIETPAIIED
                     GSVHFTFDYPVAGNKESYLLYHEELESLARNLKGLKKARFWMTFSENYLTHLRVLRNV
                     GMTRIDEVDYEGNRVIPIKFLKALLPEPASLGTKYTGKTVIGNIMTGVKDGNTITRYI
                     YNVCDHEEAFKETGTQAIAYTTGVPAMIGAMMVLTGVWRGTGVFNMEDLDPDPFMEAL
                     MKYGLPWQVVEHAPLPQEP"
     gene            complement(3250..4086)
                     /gene="speB"
                     /locus_tag="PKJ17_02440"
     CDS             complement(3250..4086)
                     /gene="speB"
                     /locus_tag="PKJ17_02440"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOD74856.1"
                     /translation="MEGVFHNRYISSRPDEGGPGIVILGCPLDVTSSFRGGSKFGPES
                     LRKASWTLETYSPYLGLDLDDMDLFDAGDLELPQGNLPGSLDRIEKSAARIIGPGRKP
                     LFLGGEHLVTYPLVKSMMGSFPLLQLVHFDAHCDLRDEYEGEKLSHATVMKRVRELGI
                     SGMHQIGIRSGTRQEFAELSPVHSPDGLAGSLERDIPIYISFDIDVFDPSLMSGVTTP
                     EPGGLMFGEVMDYLSVLKGMDIVGADLVELAPDYDTTFVSSVCAAKVAREIVMLLHSG
                     IK"
     gene            complement(4158..4640)
                     /locus_tag="PKJ17_02445"
     CDS             complement(4158..4640)
                     /locus_tag="PKJ17_02445"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOD74857.1"
                     /translation="MVTLIVRTPTRFFLVSGSSDGFSLLNAFDGALLASGVGDTNLVR
                     MSSILPPGCLEIKPRPAALPQGALVPVAYASLTSDVPGEVISAAVAIGIPEDPSLAGL
                     IMEYSARAEERVVLEQVRKMAEKGMEVRKRPIKEIMAISATFKVAAIGAVFAGVVLWD
                     "
     gene            complement(4802..>5108)
                     /locus_tag="PKJ17_02450"
     CDS             complement(4802..>5108)
                     /locus_tag="PKJ17_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOD74858.1"
                     /translation="VDLEMEGVKGFQILIPAAQNRLLDPALLELRSGERTPEGEPHEG
                     EEFGFVLSGSVELVLGGKAQKVKKGECFYYKATRKHYIINKLKATAVLLWVSSPPNF"
BASE COUNT          987 a         1599 c         1436 g         1086 t
ORIGIN      
        1 gtatctctcc cggtcgcccc cgccgctttc cgccatatcc gggtctttca tccggcggtg
       61 tgcccggtgc aactccagag ccgcctgaac cgggacctga agccgggcct caccgaagcc
      121 ggccagggcg tagccgtaac agacggggcc cccggtcctg tccgtggtat tctgtctgaa
      181 agaacgggga tgaaaataga agaggacggg gatgtccctt tctgcgagaa tggcctcggc
      241 cgcctcgggg ccgttcatgc cgtctcccgg gtcgacgccg gcgatgacga gatcgatgcc
      301 agggtccgat cggaccctct ctaaagcctc tccattagac gcaacgatga tgaccgtata
      361 gccgcatctc tcaagggccc ggcgccgcgc cccggcggtt tccgcctcga cctccacgag
      421 aagaatggtc ctgctgtgac tcatccggcc tccagcgaag atgcaatgtc aggggatcaa
      481 ttccattatc gcaaaatggc atgtgtaaat caaaagattt ttattcaacc atggagccgg
      541 tacgttcatc tcccagcgtt cgcaagccgc gggcccggca ctcctccggt cgagcctttg
      601 aaccttgaac gtgaaatccg aaaccgtatt tcacgatagt ctgttccggt agtccccgta
      661 gccgaatcgc ctgaccacct cgacccggcc gtcctttctc agcactgcta tgtcgggaag
      721 gccgatgccg ttgaatgtcg tgctcttgac gatgctgtag agggccatat ctgtgaaaag
      781 aagcctcgcg ccccgcggga gccgttcgtc aaacgagtag tctccgacca catctcccgc
      841 caggcagctt gggccggcca gccggtacgt gtgcttcttc actcccgctt cggcggagcc
      901 catgacgtga ggcctgtacg gcatgagaag gacatcgggc atgtgtgtct ccgcggaggt
      961 gtcgagtatg gctatttcca tttcattcct cacgatgtcg accacggtgg agacgaggac
     1021 ccccgcgtgg tacacgctgg cctcgccggg ctcgaggtag acctccaccc cgtaccttgc
     1081 cttaaaagca tcgataatgc ctatgaggcg ttcgaggtcg tagtcatccc tggttatgtg
     1141 atgaccgcct ccgaagttca cccacctgag gcccttcagg taccgcccgt aaagacgttc
     1201 gaaggacttg aggattcttt gaaggacatc ggaattctgc tcgcacatcg cgtgaaaatg
     1261 caggccttga acctggccgg caacggcggg aaagcggcgc tcgaactcgt cgtgcaccac
     1321 tcccagtctc gaacggggag cacaggggtt atacatcgcc gtctgaacct ccgcatgccc
     1381 ggggttcacc cgaagcccga tctggcgccc gagacgcctc gccgcctccc catacctccc
     1441 gagttggtgg aatgaattga aaatgacgca atcagaatac ctgaggaccg cccgtacgtc
     1501 cttttcagtg tatgccgcac cgaaggtgtg gacctccttt ccgaactcct cgcggcccag
     1561 ccttgcctcg ttggccccgc tggcacacac cccgtcgaga taaggcgtca tcactccgaa
     1621 ggtcgcaaag gacgcgtagg ccttcagggc gtggagtatc ctgcaccccg tcctgtcctt
     1681 cacatacctc aggacacgca tgttctcctc gataaggctc tcgtcgatga ggtaggcagg
     1741 ggtgcgtacg atgccctccg cgacaccggg agttctctct tccgcttccc ggaagacctt
     1801 cgagcgcgcc ttcatgacga gacgcgtcag tgacttcagg ccctttccgt cgagggcccc
     1861 gaggaccctg cgcctgttcc ggtcgttaag ggcatggtac atcgccgcgg cgcgggcgtc
     1921 tttcgcggag atatatttca gggcctcttc gatgatgagt ccggcaccca cgtgttcgtc
     1981 acggctcctg cggcaggggc gcgtgctcca cgacctgcca gggaaggccg tatttcatca
     2041 gggcctccat aaaggggtcg gggtcgagat cttccatgtt gaacacgccc gtgcccctcc
     2101 agacaccggt gagcaccatc atcgccccga tcatggcggg cacgcctgtc gtgtaggcga
     2161 tagcctgcgt gcccgtctct ttgaacgcct cttcgtggtc acagacattg tagatgtagc
     2221 gggtaatggt gttgccgtcc ttcacacccg tcatgatgtt gccgatgacg gtcttccccg
     2281 tgtatttcgt gcccagcgag gccggttccg gaagcagggc cttgaggaac tttatcggga
     2341 tcaccctgtt cccctcgtaa tcgacctcgt cgatcctcgt cattcccacg ttccggagga
     2401 ccctgaggtg agtcaggtag ttctcggaaa aggtcatcca gaacctggcc ttcttcagcc
     2461 ccttcaggtt cctggcgagg gattccagtt cctcgtggta caggagatag ctttccttgt
     2521 ttcccgccac ggggtagtcg aaggtgaaat gtacgctgcc gtcctctatg atcgcgggcg
     2581 tctcgatcca ttccccctgt ctccagtggc gcaccacctg cgtgacctcg cggatgttta
     2641 tctcggggtt gaagttggtg gcgaagacgt ggccgtgcgt ccccgcgttg cagtcgagga
     2701 tgtcaagata gtgtatctcg tcgaaaagat gcttccgggc gtacgctgcg aaaacgttcg
     2761 tcaccccggg gtcgaacccg gaaccgagaa gggccatgat ccccttttcg cggaaccggt
     2821 cctggtacgc ccactgccag ctgtattcaa aatgggcctc gtcgaggggc tcgtagttgg
     2881 ccgtgtctat gtagtgaacc cctgtctcga gacaggcatc catgatatga aggtcctggt
     2941 agggcagggc caggttaagg accacgtcgg gcccgaaaga ccttatgaga gcgacaagct
     3001 ccgggacgtt gtcggcgtcc accttcgccg tcccgatatc cctgccgtat ctcagcctga
     3061 tgtcgtccct gatggcgtcg cacttccgca gggtccggct ggcgatgagg atctccccga
     3121 aaacttcggg cacctgcgcg catttgtggg cggctaccgt ggcaacccct cccgccccga
     3181 ttatcatgat ccttccatgg tccttgttgt tcatagtcct tatctcctga aaagcgaatc
     3241 gatcgtggtc tatttgattc cggagtgcaa gagcatgacg atctccctcg cgaccttggc
     3301 ggcacatacg gaggaaacga aggtggtatc gtagtccggg gccagttcca cgaggtcggc
     3361 gccgacgata tccatcccct tgaggacgct gaggtaatcc ataacttccc cgaacatcag
     3421 ccctcccggt tccggggtcg tgacaccgga catgagagat ggatcgaaaa cgtcgatgtc
     3481 gaagctgata tagatgggaa tatccctttc gaggcttccg gccagcccgt cgggcgagtg
     3541 aacaggtgac agctccgcga actcctgccg tgtacccgac cgtatgccta tctggtgcat
     3601 ccccgatatg ccgagttccc tgacgcgctt catcaccgtg gcgtgagaga gcttctctcc
     3661 ctcgtattcg tccctcaggt cgcaatgggc gtcaaaatgg acgagctgaa gcaggggaaa
     3721 ggaccccatc atcgatttca caagaggata tgtgacgagg tgctctccgc cgaggaacag
     3781 gggcttgcgg ccgggcccta ttatccttgc ggccgacttt tcgatgcggt cgagggaccc
     3841 ggggagatta ccctggggaa gttcgagatc ccccgcatcg aagaggtcca tgtcgtcgag
     3901 gtcgaggccg agataggggc tgtaggtctc cagggtccat gacgccttcc tcaggctctc
     3961 ggggccgaat ttcgacccgc cccgaaaaga ggacgtgacg tcgagaggac agccaagtat
     4021 cacgatgccc ggtcctccct cgtcgggtcg ggaagatata tacctgttgt gaaaaacacc
     4081 ttccatcgca atagcctctg ggaatctccg gttacacgaa cactgccggc gtgaagccga
     4141 ccatcagagg gccgccatca gtcccacagg acgacaccgg caaacacggc gccgatggca
     4201 gcgactttaa acgtggccga tatggccatt atttccttga tgggccgctt tcggacttcc
     4261 atccctttct cggccatctt ccttacctgc tccaggacga ctctctcttc agccctggcc
     4321 gaatactcca tgataaggcc ggcaagggat gggtcctcgg gtatccctat cgccacggcg
     4381 gccgatatga cctctcccgg gacatcgctc gtaagggagg cataagcaac gggcacgagg
     4441 gctccctgcg gcagcgccgc cgggcggggc ttgatctcca agcagccggg aggcaggata
     4501 ctgctcattc tcaccagatt cgtatcgccc acacccgaag ccagaagggc tccgtcaaaa
     4561 gcgttgagca gcgaaaaacc gtcggacgag ccgctgacta gaaaaaatct tgtaggtgtc
     4621 ctgacaatca atgtaaccac ctcccaaatg gattaggcct cgcagttcca aggcatctca
     4681 tttgccggca gagttcagcc gctccacaac gcaacaggcg agagtaaacc ccgccccggt
     4741 ctttgcttgc tcgccatttt cttcccctcc ctcgcatctc ccgttctctt gcacgggaca
     4801 ttcagaaatt cgggggggaa gatacccaca acaggactgc cgtcgccttg agcttgttta
     4861 tgatgtaatg cttcctcgtt gccttatagt agaagcactc gcctttcttc actttctggg
     4921 ctttcccgcc cagcaccagt tcgacgctcc cggaaaggac aaaaccgaac tcctcgccct
     4981 cgtggggttc cccttccggg gtcctttcac ccgacctgag ttcgaggagc gccgggtcca
     5041 gaagacggtt ctgcgccgcc ggtatgagta tctgaaaccc cttcacgcct tccatctcca
     5101 gatcgacc
//
LOCUS       DAOPOA010000071         5098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17846_length_5098_cov_3.632560, whole genome shotgun sequence.
ACCESSION   DAOPOA010000071 DAOPOA010000000
VERSION     DAOPOA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17846_length_5098_cov_3.632560"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(477..1634)
                     /locus_tag="PKJ17_02455"
     CDS             complement(477..1634)
                     /locus_tag="PKJ17_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOD74859.1"
                     /translation="MEFVNAFRDLKLFGGAHAWITQSFIVVFTTLLIAFLQKLFLKRL
                     SVRLTQNRFAWDDAIVDALRGPLTLLISLVGITFAAEIVATGTNTPLFGAAVPIRSVG
                     VIIAIAWFLIRLIRAGEEKIITRSKMKGEEIDRTTADAIGKLLRISVIVTTGLVMLQT
                     LGFSVSGVLAFGGVGGLAIGFAAKDLLANFFGGLMVYLDRPFVVGDWVRSPDRQIEGT
                     VEKIGWRLCVIRTFDKRPLYVPNSTFATIALENPSRMSHRRIFETIGVRYDDAPVLKD
                     ILAGVRTMLAEHEEIAQDQLIMVYFNEYAASSLDFFIYCFTRTTVWSEYHRVKEDVLL
                     KIMDIIVAHGAQVAFPTTTLDLPDGAFKEDALNKELSRAHAPSAGLTTQRA"
     gene            1805..1939
                     /locus_tag="PKJ17_02460"
     CDS             1805..1939
                     /locus_tag="PKJ17_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74860.1"
                     /translation="MLANTQSPSAKRDIKTRQHLMERSFARSVRYGSRNAIDKYEEKS
                     "
     gene            complement(2112..3152)
                     /locus_tag="PKJ17_02465"
     CDS             complement(2112..3152)
                     /locus_tag="PKJ17_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOD74861.1"
                     /translation="MFESIFRFFSKDLAIDLGTANTLVYVKGKGITSDEPSVVAVHRD
                     SRGATKVIAVGAEAKNMLGKTPGNIVATRPLRDGVIADFEITEAMLKYFIQKVHNRKS
                     YARPRIVISVPSGITPVEKRAVKESAESGGAREVYLIEQPMAAAIGVGLPVTEPSGNM
                     IVDIGGGTTEVAVISLAGIVYCNSVRAAGDKIDEAIVQYIKRKHNLLIGERTAEMIKI
                     GIGSAYPDPDGNERTMEIKGRDLVGGVPKTLEISSTEVRQAIEEPVKAIVEAVRIALE
                     RTPPELAGDIVDKGIVISGGGAMLRNLDLLIKDVSRLPVLIAENPLTAVCEGAGQALD
                     EVGLLKEIASDF"
     gene            3816..4109
                     /locus_tag="PKJ17_02470"
     CDS             3816..4109
                     /locus_tag="PKJ17_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74862.1"
                     /translation="MKKCVVLMIAVAFVLGMVSLGSAAYEFYGIVTNVKGTKITVKNS
                     AGKTKTIQSNRQWKVGDKVRVQGNNAIEPWDGKLPPSVQRTPMGARDREIPHK"
     gene            complement(4300..>5098)
                     /locus_tag="PKJ17_02475"
     CDS             complement(4300..>5098)
                     /locus_tag="PKJ17_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOD74863.1"
                     /translation="LRYDAILIDEGQDFSDDMYRVVAGLLNPATDNLTVAIDPTQGIY
                     PGKGVGIVKKGKVHHITRVYRNTREITAFVAAFMGEERTEKKVDEGGGLFPALFESHG
                     PRPSIKGLSSEARMIEYVADTVESLVRNDGFPSSEIAILYTSKTLKTGKGEINLPEEL
                     MAALDARGILSTWASEDYRAKKSYDITTDSVTISTIHSAKGLDYACVFLAGLDFLSEK
                     MPEETMKNLTYVGLTRARYHLFIPHVRKTQLVERLLRAMQVERVKLS"
BASE COUNT         1114 a         1430 c         1382 g         1172 t
ORIGIN      
        1 accctaaagg cggacgttgc tatcaagact gccagaccgg ttcgtatccg tttcggacag
       61 accgcggcga catctgtgtc cagtgcccca atggaaaacc tagccgtgtt aatttgacag
      121 gtgatggtcg agtgttctgt tactaacggt caaagtgaca cccctgggtg gtcctccaat
      181 ctttgcgccc ggaaccttac cagaaccttt gaagaggcgg cccgtcaggg acgggccagt
      241 tgagggacgt cctgtagtcc tcctccctcc cgtaagtttt ctttccttcc catcggtcgt
      301 catcccggcg caggccggga tccaggaaat acagtgggac agaaaaaaac tctggatccc
      361 gtatcacgtc cgggatgacg aaaagaggcg tttgtcaaca ggcattcagc ggccaaattg
      421 agaagccggt tctcgcgcaa acagctttac gcagacttct ctcaaggata attctcctaa
      481 gccctctgcg tcgtcaaccc cgctgaaggc gcatgcgcgc gagacaattc cttatttaat
      541 gcgtcttcct tgaacgctcc atccggaagg tccagcgttg tggtggggaa ggccacctgg
      601 gcgccgtgtg ccacgatgat atccatgatc ttgagaagca cgtcttcctt cacacggtgg
      661 tattcggacc agaccgtcgt tctcgtgaaa cagtagatga agaagtccag cgatgaagcc
      721 gcgtattcgt tgaagtagac catgatgagc tggtcctgcg cgatctcttc gtgctccgcg
      781 agcatcgtgc ggaccccggc caggatatcc ttcaggaccg gcgcgtcatc gtagcggacc
      841 ccgatcgttt cgaagatcct gcgatgtgac attcgggagg ggttttccag ggcgatggtc
      901 gcaaaggtgg aattggggac atacagcggg cgcttgtcga aggtcctgat cacgcacagc
      961 cgccagccga tcttttcaac ggtgccctcg atctggcgat ccggcgaccg cacccagtct
     1021 cccacgacga agggccggtc gaggtagacc atcaatccgc cgaagaaatt cgcgaggagg
     1081 tctttggccg cgaaaccgat cgcaagacct cccaccccgc cgaaggcgag aacacccgag
     1141 acgctgaagc cgagggtctg gagcatgacg aggcccgtcg tgacgatgac ggagatcctc
     1201 agaagcttgc cgatggcatc cgcggtggtc cgatcgatct cctcaccttt catcttgctg
     1261 cgggtgatga tcttctcttc tcccgccctg atgaggcgta tcaggaacca ggcaatggcg
     1321 atgatgaccc cgacggacct gatggggacg gccgccccaa agagaggcgt gttcgttccc
     1381 gttgccacga tctcagcggc aaaggtgatg ccgacgagag agatcaaaag cgtgagcgga
     1441 ccgcgcagag cgtctactat ggcatcatcc caggcaaaac ggttttgcgt gagacggacg
     1501 gacagtctct tcaagaagag cttttgaaga aacgcgatca acagcgtcgt gaaaacaacg
     1561 ataaatgact gcgtgatcca ggcgtgcgcc cccccgaata gcttaagatc ccgaaaagca
     1621 tttacaaatt ccatgcggac tccttcgttt gtttgcgcgc gttgaaccgg aagttacagg
     1681 cgatgaaaca aggacacctt cggttttcaa taatttcgct tatggaaaag aggtatgtca
     1741 agggaaaggg gaagccggga gccgctcggt ccagcgtcat gcacggaagg agaacctcga
     1801 cgagatgctc gcgaacacgc agagcccttc ggcgaagcgg gacataaaga cccgccagca
     1861 cctcatggaa cggtccttcg cacggagcgt ccggtatggt tcaagaaacg ccatagacaa
     1921 atacgaggaa aaatcttaga agtcggatga tgatgggtag gatgtcagat tgacttgccg
     1981 cttattaaga ccaagtttct ttcggctcct tgatgtaggg taacaccaat ttatcgacat
     2041 tcccatacat aaaacgacga tgcaagacgg caattggttt tttgtctccc ccatacggcc
     2101 cggacttgct ttcaaaaatc cgatgcgatt tctttcagaa gcccaacctc gtcaagggcc
     2161 tgacccgcgc cctcgcaaac cgctgtcagg gggttttcgg cgatgagaac agggagcctg
     2221 ctgacatcct tgatgagaag gtcaaggttt ctgagcatgg cgccgccgcc gctgatgacg
     2281 atgcccttgt cgacgatgtc gcccgcgagt tccggcgggg tcctttcgag ggctatcctt
     2341 accgcttcca caatggcctt gacgggctcc tctatggcct gccgtacttc cgtcgatgag
     2401 atctcaaggg ttttcgggac accgcccaca aggtcccggc ccttgatctc catggtgcgc
     2461 tcatttccgt cggggtccgg ataggccgaa cctatgccta tcttgatcat ctccgccgtt
     2521 ctttctccga tgaggaggtt gtgtttcctc tttatgtact ggacgatcgc ctcatctatc
     2581 ttgtcgccgg cggccctgac ggaattgcag tacacgatgc cggcgaggct gatgacggca
     2641 acctcggtcg tgccgcctcc gatgtccacg atcatgttgc cgcttggttc cgtaacggga
     2701 aggcctacac cgatagccgc cgccatcggc tgctcgatga gatacacttc tctcgcaccc
     2761 cccgattcgg cggattcctt cacggcccgt ttttcaacgg gcgtgatacc cgaagggacg
     2821 gagataacga tccgcggccg tgcataggat ttcctgttat ggaccttctg gataaaatac
     2881 ttgagcattg cctcggtaat ttcaaaatca gcgatgacac cgtccctgag cggccgcgtc
     2941 gcgacgatgt tgcccggtgt cttgccgagc atgttcttcg cctccgcacc gacggcgatg
     3001 accttcgtcg ccccccggga atctctgtgg acagccacca ccgatggttc atccgaggtg
     3061 atccctttcc ctttgacata taccagcgtg tttgcggtgc cgagatctat tgccaggtct
     3121 ttcgagaaaa agcgaaaaat cgattcaaac attaatccct cttccggctc aggcgcatga
     3181 tcctttccgc aaatgacatt ctatatagag ctgccggttc ctgaatacat caatcacctg
     3241 gacctcattc tttgcagtgt attttcttcg gatgacatcg tctggctgaa atgcttacag
     3301 gtctccagac tccttccccg gacctactat agcaagatga gtgccatctc cggtccccgc
     3361 cggattgtat cgaaatagcc cgtttttcga caaagatccc cctgcgtcag accgacaggt
     3421 gaaggtattt acctgcacca accaacccgc agtgaaggat attccataca cgcagcctcc
     3481 cgtagcctct gtggacgacg tcgccggctc tcttttgatc aggcgtcccc agtgtttcca
     3541 tgcgtgtcac ggaaaatcgg agggacatcc ccttaccgcc tccattcatc acgcctcaag
     3601 tttaggtggt cttaagaagt tacttgtttt tcttggagcg tccccttgcc tctacccctt
     3661 acctctaccc cgtagtcctc ctggcttccg gcatattgct tgaattttct caataccgat
     3721 gataaaagat tgaagtacaa ggcatgagac atcggctgat attctccaaa ggaacactgt
     3781 aagggtcttt aatttcatct taaggaggtc gggcaatgaa aaagtgcgtc gttcttatga
     3841 tagcagttgc gtttgttttg ggcatggtca gtctgggatc tgctgcgtac gaattttacg
     3901 ggatagtaac aaacgttaag ggtacaaaaa tcacggtaaa gaattccgcg ggcaagacaa
     3961 agaccatcca gagtaatcga caatggaagg taggcgacaa ggttagggtg caaggcaaca
     4021 atgcgataga accctgggat ggcaaactgc ccccttccgt ccagcggaca cctatgggcg
     4081 caagagatcg ggagatccct cacaaatgat ttgacgcagg atttggcctt cggccgcatc
     4141 aaacccaatc gggaggatcc gggacgttct caagagatca gactatctca gcgtgtcctc
     4201 tagggatgtt gagatgttac gaatgataac gtgatttctg aagagcgtcc ccttgcctct
     4261 cttcaaatcc attagccaaa gcaaccaggc gtcgccaatt caggacagtt taaccctctc
     4321 cacctgcatg gctctcaaca accgctctac cagctgtgtt ttgcgcacat gcggaatgaa
     4381 caggtggtag cgggcgcggg tgaggccgac gtaggtcagg ttcttcatcg tctcttcggg
     4441 catcttttcg ctcaggaaat cgaggcccgc gaggaagacg caggcgtagt cgaggccttt
     4501 cgcgctgtgg atggtggata tggtcacgct gtccgttgtg atgtcgtagg acttctttgc
     4561 gcggtagtcc tcggatgccc aggtggagag gatgcctcgt gcgtcgagcg cggccatgag
     4621 ctcttcgggg aggtttatct cgcccttccc ggtcttcagc gttttcgacg tgtagagtat
     4681 ggctatctcc gaagaaggga aaccatcatt ccgcacgagg gactccacgg tgtcggccac
     4741 gtattcgatc atccgcgctt cgctggaaag tcccttgatc gaaggcctgg gcccgtggga
     4801 ctcgaaaagt gccggaaaga gaccgccgcc ttcgtcgacc ttcttctctg tgcgttcttc
     4861 tcccatgaag gcggcgacga atgcggttat ctcccgtgtg ttccggtaga cacgagtgat
     4921 gtggtgcacc tttcccttct tcacaatccc cacacccttg ccggggtaga tcccctgcgt
     4981 cgggtcgatg gctacggtga gattgtctgt cgccggattg aggaggcccg cgaccacccg
     5041 gtacatgtcg tcgctgaaat cctgaccctc gtcgatgagt atggcgtcgt acctgagg
//
LOCUS       DAOPOA010000072         5092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17868_length_5092_cov_3.338892, whole genome shotgun sequence.
ACCESSION   DAOPOA010000072 DAOPOA010000000
VERSION     DAOPOA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17868_length_5092_cov_3.338892"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKJ17_02480"
     CDS             <1..456
                     /locus_tag="PKJ17_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74864.1"
                     /translation="SGKKAHARDYIEEGLDTLSNMRMCGNMAGQLAIQTALGGYQSLQ
                     EMISPGGRLYEQREAAYDGFRAIPGITCVKPMGALYLFPKIDVKKYNIKDDQKFLLDF
                     LTEKKVLMVQGTGFNWPAPDHFRVVFLPSVEEIKSVAQRMAEFLKEYTQ"
     gene            complement(640..2535)
                     /locus_tag="PKJ17_02485"
     CDS             complement(640..2535)
                     /locus_tag="PKJ17_02485"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOD74865.1"
                     /translation="MAGIYSALSKKLADREGLYLVGSVEEQRLFLCEADRVMETYPAS
                     TSRLGVGIREDSLKTPPGLHRVCEKIGSGAPLGRVFRDRRDTGTDWDGVSREDNLILS
                     RILRLEGLDEGINRGPGVDSRERYIYIHGTNREDLVGTPFTHGCLTLRNTDMADLFER
                     VPEGTLVYIDPPALVVAERSCRKVHFTGIFGTGMSALAQYLRFEGIGVSGSDRLLESE
                     DTASVRRSLEGLGCRIASQDGTGVTEDADALCVSTAIEETNPDIAAARARGIPVIHRS
                     DLLASIVSSKKTVAVAGTSGKSTVTAMIFEFLSACGMSPSLVSGAPLVRLEAQGLIGN
                     AYSGGSDLLVVEADESDGTLVKYSPAIGVVLNISKDHKGIDEIQGLFATLISRSSWTA
                     SNADDPLLATLPATVRFGRDGAASWHPDGEELLAASVRLTRAGVDYHLPLPGEHNLEN
                     LRAALCVCEHLGCGGPALAEAVRGFSGVARRFSVTNTGQGITVVDDFAHNPAKITAAV
                     TAARGLAGRIIAVYQPHGFGPTRFLKDEYIAAFRTLFQERDSLYLLPIYYAGGTAQKD
                     ISSQDIIDGLGPVPFHAEAVQDRKQLLERLSADAKQGDCIIVMGARDPSLPALVRKII
                     EMFEGPF"
     gene            2874..3392
                     /locus_tag="PKJ17_02490"
     CDS             2874..3392
                     /locus_tag="PKJ17_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74866.1"
                     /translation="MRKTGVWIAVAIILVVASVMVAAAGDTRAPSARGPQWDSWNPRN
                     NEDMGSPAFRPAASNTMVLQYGFRGALVYEPNGNRAERLRMVTITRGKVEGFVNQVND
                     RLALAAGLHEIAVYDFSKHKWVVLERVTPDDSTGALKQNFILTPDYAMVKQLGGPFYK
                     YTTATGWQKQAR"
     gene            3473..3910
                     /locus_tag="PKJ17_02495"
     CDS             3473..3910
                     /locus_tag="PKJ17_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74867.1"
                     /translation="MALQEDIRQVFSAFPAEFSEKKGVVTAEYVVAERKSFLSKKKVT
                     YIAKYRIVEDAKEVRFTEMLKESGSGFSSSGLDDGPSGFGFKKTTYKTGMGGREGTIE
                     EQSSLLGSKYSYTFDFKATRAAVQKKVEDAGYAFKYQITPIGL"
     gene            4077..4730
                     /locus_tag="PKJ17_02500"
     CDS             4077..4730
                     /locus_tag="PKJ17_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74868.1"
                     /translation="MKRFMFLGVLAAFCFVLSIGSGVAAAQSVHDRADADKDGVITKE
                     DMKKALEERYRGEDTNKDGKVTVDEYQDARRKNFDDADANKDGMVTVEEWAIYWCGTD
                     QGAAKVKKPVKMSRNASRAKLMDTNKDGKLGKDECVVFWAGRFVDLDGNKDSKMSRQE
                     YLDRMKAMAKMMDIDGDGVITIEEYSVSWVGKDNAATKNEPAGKTKKGAAQKTPAKK"
     gene            4853..>5092
                     /locus_tag="PKJ17_02505"
     CDS             4853..>5092
                     /locus_tag="PKJ17_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74869.1"
                     /translation="MRDTKYMAIPPGAILPGSLPKFKIYVLSPDGRYILWARSGNEVN
                     RDRLVTLAETGQEEVYIDLDEEIKYEEYLENNLGII"
BASE COUNT         1171 a         1342 c         1577 g         1002 t
ORIGIN      
        1 agcggtaaga aggcacatgc ccgggattac atagaggaag ggctcgacac cctgtcgaac
       61 atgaggatgt gcggcaacat ggcgggccag ctcgccatcc agaccgccct tggcgggtat
      121 cagagcctcc aggagatgat atcccccggc ggccgtctct acgagcagag ggaggccgca
      181 tatgatggtt tccgcgcgat accggggata acctgcgtca agcccatggg tgccctatac
      241 ctctttccaa agatcgacgt taagaagtac aacataaagg acgaccagaa attcctcctc
      301 gatttcctga cggagaaaaa ggtcctcatg gtgcagggca cgggtttcaa ctggcccgcc
      361 ccggaccatt tccgcgtcgt cttcctcccc tccgtcgaag aaataaagag cgtggcgcag
      421 cggatggcgg aatttctcaa ggagtatacg caataaaaac agttttaggt tttaggtttt
      481 aagttttaag ttaagaaaaa ccgttttagg tgttaggttt taagttttag gtaaaagacc
      541 ggaaaagcca ggtcctcttc cggcaaatag gtgggcaccc aaccaccact ctgttcgcct
      601 tggtcttcca cttaaaactt aaaaccgtct tgttagcact taaaacggtc cttcaaacat
      661 ttctattatc ttcctgacaa gagctggcag ggatgggtcc cgggcgccca ttactatgat
      721 gcagtcgcct tgcttcgcgt cggcggagag cctttcgagc aactgtttgc ggtcttgaac
      781 tgcctcggcg tggaagggga cggggccgag tccgtcgatg atgtcctgcg aggagatgtc
      841 cttttgggcg gtgccgccgg cgtaataaat gggaaggagg tagaggctgt cacgttcctg
      901 gaagagggta cggaaggcgg cgatgtattc gtccttaagg aagcgtgtcg gtccgaaccc
      961 gtggggttgg tagacagcga tgatacgacc ggcgaggccc cgggcggcgg tcacggcggc
     1021 ggttatcttt gccgggttgt gggcgaaatc gtcgacgacg gtgatgccct gccccgtgtt
     1081 cgtcacggag aagcggcggg cgacgccact aaaccccctg actgcctcgg cgagggcagg
     1141 cccgccgcag ccgaggtgct cgcagacgca cagggccgct cggaggttct cgaggttgtg
     1201 ttcccccgga aggggcagat ggtagtcgac gcccgcgcgg gtgagcctga ccgacgcggc
     1261 aaggagttct tctccgtcgg gatgccacga ggctgctccg tcacgaccga acctcaccgt
     1321 tgccggaagg gtggcgagaa gagggtcatc ggcattgctc gcggtccagg aggaccgcga
     1381 tatgagtgtc gcgaaaagtc cctggatctc gtcgatgccc ttgtggtcct ttgagatatt
     1441 gaggacgacg ccgatcgcag gtgagtactt gacgagcgtc ccgtcgctct catccgcttc
     1501 gacgacgagc aggtccgaac cgccggagta cgcattgccg atgaggccct gcgcttcgag
     1561 cctaacgagc ggtgcaccgc tgaccagcga cggggacatc ccgcacgccg aaaggaattc
     1621 gaatatcatc gccgtcaccg tcgacttgcc gctcgtgccg gcgacggcga cggttttttt
     1681 tgaagagacg atcgacgcga gaaggtcgga gcgatgtatg acgggaatgc cccttgcccg
     1741 cgcggcggcg atgtcggggt tcgtctcttc gatcgccgtg gacacgcaga gggcatcggc
     1801 atcctccgtc acaccggtcc cgtcctggct cgcgatccta cagccgagcc cctcgaggga
     1861 ccgccgcacg gaggcagtat cttcactttc caacagacgg tccgatccgg aaacgccgat
     1921 cccctcaaag cgcaggtact gggcgagggc gctcatcccg gtgccgaaga tgccggtgaa
     1981 gtgaaccttc cggcaggatc tttccgcgac gaccagggcg ggagggtcga tgtagacaag
     2041 ggtgccctcg gggaccctct cgaagagatc tgccatgtcg gtgttgcgaa gggtcaggca
     2101 tccatgggtg aagggagtgc cgacgaggtc ttcgcggttg gtcccgtgga tgtagatgta
     2161 gcgctcgcgg gagtccacgc cggggcccct gttgatcccc tcgtcaagac cctcgagacg
     2221 gaggatacgg gacaggatga ggttgtcctc tcttgagacc ccgtcccagt cggtccctgt
     2281 atctctgcgg tctctgaaga ctcgtcccag gggtgcgccg gacccgatct tctcacagac
     2341 gcggtgaagg ccgggaggcg tcttgaggga atcctcgcgt atgccgacgc cgagtcgcga
     2401 cgtggaggca ggataggttt ccatgacgcg gtccgcctcg cagagaaaga gacgctgctc
     2461 ctccacagac ccgacgagat acaatccttc ccgatcggca agcttcttcg acagtgcact
     2521 ataaattccg gccaaccgaa atccgtccat cttgcccctt tgcatcttgc attattgatg
     2581 tggcaattat tgataccatc ggcggtgtgg ggattcaaga ggaacttcgc cgtgtcggaa
     2641 tgtcagatct tgtcctgccg gtggataaca tcgtgcacag agcatgtcaa aaccgctcaa
     2701 ggagcatcgt gacgcacaga aaacgggagg atgcaaatgt cttttgacat gttcctacct
     2761 ccctgctata tttgtcgaat catgtatgac caagcctttc actccgcggc cggagcatcg
     2821 aaggcggagg ataccgacat tgctatcaac acacaacgaa aaggaggagt ttcatgagga
     2881 aaacaggggt ttggatcgca gttgcaatta tccttgtggt tgccagcgtc atggttgccg
     2941 ccgcgggcga cacgagggcg ccttctgccc ggggtcccca gtgggacagc tggaatccca
     3001 gaaacaacga ggacatgggc agtccggctt tcagaccagc agccagcaat accatggtcc
     3061 tgcaatatgg ctttcgggga gcgctggtgt atgaaccgaa cgggaacagg gcggagaggc
     3121 tcaggatggt gacgatcacc cgcggcaagg tggaagggtt cgtcaaccag gtcaacgaca
     3181 ggctcgcgct cgcagccggc cttcacgaga tagccgtcta cgacttcagc aagcacaaat
     3241 gggtcgtcct cgagagggta acccccgatg attcgacagg agccctgaag cagaatttca
     3301 tcctcacccc cgactacgcc atggtcaaac agcttggcgg gccattctac aagtacacaa
     3361 cggcaacggg ctggcagaaa caggcgcggt aagggtgcac cgttcatcga caggcaggtc
     3421 ccggccaggc ttttcccgcc cggccgggcg aattcaagat aaggagacat ggatggcact
     3481 gcaggaagat atcaggcagg ttttttccgc cttcccggcg gaattctctg aaaagaaggg
     3541 tgttgtcacg gccgaatatg tcgttgccga acggaaatcc ttcctttcga aaaagaaggt
     3601 gacctacatc gcgaaatacc ggattgtgga ggacgcgaaa gaggtccgct tcacggagat
     3661 gctcaaggaa tcgggctcgg gcttttcgag cagcggtctc gacgacggtc cttcgggttt
     3721 cggtttcaag aaaacgacat acaagacggg tatgggcggc cgggagggca ccatagagga
     3781 gcagtcgagc cttctgggaa gcaagtacag ctacacgttc gatttcaagg cgacacgggc
     3841 cgccgtccag aagaaggtcg aggacgccgg ctacgccttc aagtaccaga taacgccgat
     3901 aggattgtaa ggaatatggt tttaagtttt aggttttaag ttttaggtga aagcttttcg
     3961 ctgtgccgga ttagagaagc tgttaccgtc cggggctcag cgagattgga acggtcgaat
     4021 gtttatacct aacacttaaa acctaaaact taaaaccgtc tttgaaagga gagattatga
     4081 aaagatttat gtttcttggt gttttggccg cattctgttt tgttctttcc atcgggtccg
     4141 gggtcgcggc ggcacaatct gttcatgaca gggctgatgc ggacaaggac ggggtgatca
     4201 cgaaagagga tatgaagaag gccctcgagg agcggtacag gggcgaggat accaacaagg
     4261 acgggaaggt gaccgtcgac gaataccagg atgcccgccg gaagaatttc gatgatgccg
     4321 atgcgaacaa ggacggcatg gtcaccgtcg aggaatgggc catctactgg tgcgggacgg
     4381 atcagggtgc cgccaaagtg aagaaaccgg tgaaaatgag caggaacgca tcgagggcaa
     4441 aattgatgga caccaacaag gatggcaagc tgggcaagga cgaatgcgtg gtgttctggg
     4501 cgggccgctt tgtcgatctt gacggcaaca aggacagcaa gatgtcccgc caggaatacc
     4561 tcgacaggat gaaggcaatg gccaagatga tggatatcga cggtgacggc gttatcacca
     4621 tcgaggaata ctctgtatcc tgggtgggga aggataacgc cgcgacaaag aacgagccgg
     4681 ccggaaagac gaagaaaggg gcggcgcaga agacgcccgc aaagaaatag aggggaggcc
     4741 gcaggacctt tcctctcctt taagctttca ttacgagatg cgataatcct gttgacctgc
     4801 gacggaactg ccatgtccgt ctcacgaatt acggtcaggg gagaagcatc tcatgcgcga
     4861 tacaaaatac atggccatac ctcccggcgc gatactgccg ggaagcctgc ctaagtttaa
     4921 gatatacgtc ctttcgcccg atgggcgcta catcctctgg gcgcgctcgg gcaacgaggt
     4981 gaacagggac cggctcgtca cgctggccga aacggggcag gaagaggtct acatcgacct
     5041 cgatgaagag atcaagtacg aggagtatct cgagaacaac ctggggatca tc
//
LOCUS       DAOPOA010000073         5086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17901_length_5086_cov_3.376267, whole genome shotgun sequence.
ACCESSION   DAOPOA010000073 DAOPOA010000000
VERSION     DAOPOA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17901_length_5086_cov_3.376267"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PKJ17_02510"
     CDS             complement(<1..613)
                     /locus_tag="PKJ17_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOD74870.1"
                     /translation="MMDPGNQVVLIDTHCHLNDASFTDVLDDVLDRARREGVVQFVVP
                     AYDRPSLSRTLELARRFPGVVFPAFGIHPWYLEDGGLEGLEGYLSLQETVAVGEVGLD
                     LAPGMAPVEAQEGAFVAQLDMAVERGLPVLVHCRKAHERMFNILGAYRGKVTVVLHSF
                     SGSAEVMERFLDLGCYISFSGSVTRQGAKKYHRCARLVPAERFL"
     gene            complement(849..1079)
                     /locus_tag="PKJ17_02515"
     CDS             complement(849..1079)
                     /locus_tag="PKJ17_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74871.1"
                     /translation="MDYDEDKIDDHTAKKRWNELPEQARKDIIESVWCPHCGTGVPME
                     LRDGKMEGRLLVLRGTCKKCGGEVARVVEPEG"
     gene            1367..1798
                     /locus_tag="PKJ17_02520"
     CDS             1367..1798
                     /locus_tag="PKJ17_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74872.1"
                     /translation="MAVWVLFFTASSADAVRLPEFYARMAEVSKIKATAVVTSVEIIE
                     RTEHSTFKKVVFALRHSFSDAVPTTFSGTCYSIDHSWQEPAAGGTIYHYPRKGEKVYV
                     TIGADGGSITSYTAVDETLEKMFIQNPGKIRYGMGKAYLEK"
     gene            complement(1810..2010)
                     /locus_tag="PKJ17_02525"
     CDS             complement(1810..2010)
                     /locus_tag="PKJ17_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74873.1"
                     /translation="MFLVSFYMSARVKAHYGQAFAAVIGPLVEVPVLVNLVHLAVRFK
                     KKYFPHAVETPTGICYYTCKDR"
     gene            complement(2209..2577)
                     /locus_tag="PKJ17_02530"
     CDS             complement(2209..2577)
                     /locus_tag="PKJ17_02530"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOD74874.1"
                     /translation="MKELLSVFKALSDETRLRIVKLLENGELCVCHIVAAVDMSQSRV
                     SFHLKILKDAGLVKDRREGKWMHYRLNESDLFKRLLFLSIVERVKEEDMAGDRQRLEA
                     FIASRPEEFAGISTACCGRV"
     gene            2721..3839
                     /gene="hgcA"
                     /locus_tag="PKJ17_02535"
     CDS             2721..3839
                     /gene="hgcA"
                     /locus_tag="PKJ17_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_252989029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2769..2771,aa:Sec)
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HOD74875.1"
                     /translation="MKPLYSSLVMLDNAPCUGPSEKRGPVRPPRLDQPFVTGVIKTAA
                     GPVPRVSKVFSGTDRIGAFKVRWGIRRTRYRVDAGLYAIGEPTAESPVFVTANYKLTF
                     DLLRRAIASIDGWILVLDTLGINVWCAAGKGTFGTDELVERIEASRLAGIVNHRKLIL
                     PQLGGPGVAGHEVKRRSGFQVVYGPVEAEEIPLFLEKGLKANERMRRKDFGFMERLAL
                     VPMEVVPAVRLGLPVAAGLFALCFLLGGLSLSRALECFAVPAGAIGTSILAGTVLTPV
                     LLPWLPGRAFSVKGLVTGILSALLLIPLVRGSGVLETAFLLVSIPAMSAYLAMNFTGS
                     STFTSLSGVKKEMRFALPLEIGGVGAGLLMWIIALLGG"
     gene            3859..4131
                     /gene="hgcB"
                     /locus_tag="PKJ17_02540"
     CDS             3859..4131
                     /gene="hgcB"
                     /locus_tag="PKJ17_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_250697394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HOD74876.1"
                     /translation="MVYLKDVVTLTHIPELCSGCGICLSVCPREVLQRLNGKVRIARR
                     DACIECGACQRNCPHGAVTVKAGVGCASALINQMLGRKKACCVVGD"
     gene            4295..4567
                     /locus_tag="PKJ17_02545"
     CDS             4295..4567
                     /locus_tag="PKJ17_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74877.1"
                     /translation="MSTRSLTMLLALAALMSVASCASFDCAYDVKARPEPVYLAAEEG
                     VFYGQNYTACFVIDGVVHKEYIPECAYRYYYDRQNQRRSAAPATRQ"
     gene            4857..>5086
                     /locus_tag="PKJ17_02550"
     CDS             4857..>5086
                     /locus_tag="PKJ17_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOD74878.1"
                     /translation="MKDPSGTTRELIEEIASLKERIRELERLEGRRTEEALRESERKF
                     RDLAEKSIAGIYVLRPDGVVRYVNARCAEIFG"
BASE COUNT         1073 a         1471 c         1441 g         1101 t
ORIGIN      
        1 ggagaaaccg ctccgccggc acgagccgtg cgcagcgatg gtacttcttc gccccctgac
       61 gcgtcacgga accggaaaag gagatataac atcccaggtc caaaaacctc tccatcacct
      121 cggcgctccc cgaaaaggaa tgcagcacca ccgtcacctt cccccgatag gcaccgagga
      181 tattgaacat cctctcatgc gccttcctgc agtgaacaag caccggcagc cccctctcaa
      241 ccgccatatc gagctgagcg acaaacgccc cctcctgcgc ctcgacgggc gccatccccg
      301 gcgcaagatc aagccccact tctcccactg ccactgtttc ctgcaggctc aggtaaccct
      361 ccagcccctc cagacctcca tcctccaggt accacggatg gatcccaaag gccggaaaga
      421 caacacctgg aaaacgccgc gcaagttcaa gcgtccggga cagcgaggga cgatcgtagg
      481 caggcacgac gaactgcacg acaccttcgc gtcgggcgcg gtcgaggaca tcgtcgagga
      541 cgtccgtaaa ggacgcgtcg ttgaggtgac aatgggtgtc gatgagaacg acctgattcc
      601 cgggatccat catgctgcaa gtgtactaaa gacggttgca ggtttcaagt ttcagaaaaa
      661 gggtggatgc gagttacgcg ctgcgggtta cgggttagaa gcgaaaggac ggtgcaggtt
      721 gggggctttg ttcgttttgg tgtaaggttg gtgcgggcaa tcgcgcgggg tgctggagaa
      781 atgcgcaacc tacggttcag caagggatgt ttttggacga ttcacttaag cctcttgatt
      841 cggcaacctc atccttctgg ttctaccact cgtgccactt caccaccaca tttcttgcat
      901 gtaccccgca gaacgagcaa ccgcccttcc atcttcccat cgcgcagttc cattggcaca
      961 cctgttccac aatgtgggca ccagaccgac tcgatgatgt cttttctcgc ttgctccggc
     1021 agttcgttcc atcgtttctt tgcggtgtga tcatctatct tgtcttcgtc gtagtccatt
     1081 caccctccca ggtctcagcc gagagccgcc agtcccttgc gatcgatgag gtcgagcagg
     1141 cgctgggcgg gaccgcttgg tttcttctgt ccctgttccc actgctgtac cgttgactca
     1201 caggtctcct taagaccagc tcaggatttt tgtagaacca ggatgaacat gctgttagac
     1261 ttgttcacgg ggaatctccc tcatgcttgc ttgccaagga gagaagcttt tgagacattt
     1321 catatcgaga atgggaacat cgacggtttt caggatggtt tcggttatgg ctgtgtgggt
     1381 cctgttcttc accgccagtt ccgccgacgc agtcaggttg ccggagttct acgcgcgtat
     1441 ggccgaggtg tcgaagatca aggcgactgc ggttgtgaca tccgtggaga tcatcgaacg
     1501 tacagaacac agcaccttca agaaagtggt ctttgcattg aggcactcct tcagcgatgc
     1561 cgtcccaacc acattcagcg gtacctgtta ttccatcgac cattcatggc aggagccggc
     1621 agcgggtgga acgatctatc actatcccag gaaaggtgag aaagtctacg tcacgatcgg
     1681 cgcggatggc ggaagcatca cgagctacac cgccgttgac gaaaccctgg agaaaatgtt
     1741 cattcagaat cccggaaaga tccgctatgg aatgggaaag gcgtatctgg agaaataacc
     1801 gggctcacat taccggtctt tgcaggtgta gtaacagata cccgtcgggg tttccacggc
     1861 gtgggggaag tatttctttt taaaccgaac ggcaaggtgg acaaggttga cgagaacggg
     1921 gacttcaacg aggggtccga tgaccgccgc gaaggcctga ccgtaatggg ctttcaccct
     1981 ggcgctcatg tagaacgaca cgaggaacat gacgacaaag tagatcagcc atgaggataa
     2041 gaccgatagc gatgggtatg tttgtggtcc cggactggaa ccggttccag aagtcaacca
     2101 cgccgggatt gaggtatccc caggcaacgc cggcgaacat cgccagaaag atccagaggg
     2161 tcagaaaacg atccaagaag gaaagtctta cagccggacc acccacgctc acaccctgcc
     2221 gcagcacgcg gtcgagatgc cggcgaactc ttccggccgt gacgcgatga atgcttccag
     2281 acgttgacga tcgcccgcca tatcctcctc tttcacccgt tcaacgatgg aaaggaagag
     2341 aaggcgcttg aacaggtccg actcgttgag gcggtaatgc atccatttgc cttcccgcct
     2401 gtccttcacg agccccgcgt ctttcaggat cttcaggtga aaggaaaccc tcgactgaga
     2461 catatcgaca gccgccacga tatgacatac acagagttcg ccgttctcga gaagcttcac
     2521 tatcctcagc cgcgtttcgt cggacaacgc cttgaacacc gacagaagct ctttcattcc
     2581 ggcctccctt catatcaagt attatagata tgtatatagt acgattgtcc tgatatgtca
     2641 acgtgttatt ttccaacact tcaacgtgtc ttgatatgat tgccttgaca ggagcctcca
     2701 catgctctat catggattat atgaaacctt tgtattcctc tctcgtcatg ctcgataacg
     2761 cgccttgctg aggcccgtcg gagaagcggg gtccggtgag accccctcgt ctcgaccagc
     2821 cttttgtcac tggcgtgatc aagaccgccg ccggtcccgt cccccgggta tcgaaggtct
     2881 tcagcggaac ggatcgcatc ggggccttca aggtgcgatg ggggatcagg cggacgcggt
     2941 acagggtcga cgcgggcctt tacgcgatag gtgaaccgac cgcggaatct cccgtctttg
     3001 tcacggcgaa ctacaagctg actttcgatc tcctccgaag ggcgatcgcg tcgatagatg
     3061 gatggatcct cgtactcgac acgctgggga tcaacgtgtg gtgtgccgcg gggaaaggca
     3121 ccttcgggac cgacgaactg gtggagcgca tcgaagcgag ccgtcttgcc ggtatcgtga
     3181 accaccggaa gctcatactt ccccagcttg ggggaccggg ggtcgcgggc cacgaggtga
     3241 aaaggcgctc cggctttcag gtggtctacg gacccgttga ggctgaagag atacctctat
     3301 tccttgaaaa aggcctcaag gcgaacgagc ggatgcggag gaaggacttc ggcttcatgg
     3361 aaagactggc gcttgttccg atggaggtcg taccggccgt gaggttgggg cttcccgttg
     3421 cggcaggtct cttcgccctt tgcttccttc ttggggggct ttcgctatcg agagctctcg
     3481 aatgttttgc ggtccccgcc ggggccatcg gaacttccat actcgcgggc acggtgctca
     3541 ctccggtcct gcttccctgg cttccgggcc gggccttttc ggttaaaggg cttgtgacgg
     3601 gcatcctgtc ggcacttctg ctcattcccc ttgttcgcgg ctcaggtgtg ctggagacag
     3661 catttcttct tgtctctatt cccgccatgt ctgcctatct cgccatgaat ttcaccggtt
     3721 cctccacatt cacttcgctt tccggcgtta aaaaggagat gcgcttcgcc cttcccctcg
     3781 agatcggggg cgtaggggca ggcctcctga tgtggatcat tgctctcctg ggagggtaga
     3841 gggacaagaa tgcgacccat ggtctatctc aaagacgttg tcacgctgac ccacataccg
     3901 gagttgtgca gtggctgcgg gatctgcctt tccgtgtgcc cccgtgaggt gcttcaacgc
     3961 ctcaacggaa aggtcaggat cgcccggcgt gacgcctgca tcgagtgcgg cgcctgccag
     4021 cgcaactgcc ctcacggagc ggtgaccgtc aaagcgggcg tcggctgcgc ctcggccctc
     4081 atcaaccaga tgctcggcag aaaaaaggcc tgttgcgtcg tcggagattg acggacagag
     4141 cgtggatgat caccgcgtcg ttcccccaac ctccatcccg ggcaaacgcg gccgcagttg
     4201 tccctgtcgc cccgtcatgg cttgttccgg gaaggccgtg gttacccctg catcggatat
     4261 ttgatagaat agagatatgt ccggaggtgg aaccatgtct accagatcat tgacaatgtt
     4321 gttggcgctg gcagccctga tgtctgtcgc gtcctgcgct tcattcgact gcgcgtacga
     4381 tgtcaaggcg cgtccggaac cggtttacct cgccgccgag gaaggcgtct tctacgggca
     4441 gaactacacg gcgtgttttg tgatagacgg cgtcgtccac aaggagtaca tacccgaatg
     4501 cgcgtaccgc tattactacg acagacagaa ccaacggcgg tccgccgcgc ctgcaacgcg
     4561 tcaatgacgg cggcgctttc gggaacaccc ctcccatctt cgcatccggc caccttttga
     4621 ggttctcgag aatcagagac cggggcagtt gtctttcggc cacctttccc cgcccctcat
     4681 gctccggggg catcgcgcga taatcataac ggagaggacg tcacgtcaac agccgaccgc
     4741 tgccgcgaca cgcgatcgtt ctttacggag atttgggaat tgacaacaac gctgtctatg
     4801 ataacatcgt gtatgccttt acatcagtgc atcgtcggac tcgtgaaacg ggacgcatga
     4861 aagacccgtc gggaacgacc cgggaattga tcgaagagat agcctcttta aaggagagga
     4921 tcagggaact ggagcgtctg gaaggcaggc gcaccgagga ggcgctcagg gaatcggagc
     4981 gcaagttccg ggaccttgcc gagaaatcca tcgccggtat ctatgtcctg cggccggatg
     5041 gggtggtgcg gtacgtcaac gcgcggtgtg ccgaaatatt cggcta
//
LOCUS       DAOPOA010000074         5074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_17959_length_5074_cov_4.577406, whole genome shotgun sequence.
ACCESSION   DAOPOA010000074 DAOPOA010000000
VERSION     DAOPOA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17959_length_5074_cov_4.577406"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2228
                     /locus_tag="PKJ17_02555"
     CDS             <1..2228
                     /locus_tag="PKJ17_02555"
                     /inference="COORDINATES: protein motif:HMM:NF009414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional dihydroorotate dehydrogenase B NAD
                     binding subunit/NADPH-dependent glutamate synthase"
                     /protein_id="HOD74879.1"
                     /translation="EKKVLAPSITMYKLYVPDIARKVKAGQFVVVRGDDMGERIPLTV
                     ADYDTKKGTITIIFQEVGTSTQKLAKFEAGMALLDVVGPLGKPSHIEKFGTVVCVGGG
                     VGVAPVLPIAKALKEAGNEVISIIGARTQSMLILEEEMKKASTTVHVTTDDGTYGHHG
                     FVTDVLKKIIEERGAANIALVVGIGPVIMMKAVTDVTRPFGVKTVVSLNTIMVDGTGM
                     CGCCRATIAGETKFVCVDGPEFDGHAVDFPELVLRQKMYNREERRALWDHKCKIDAQE
                     KAVKKARKRVKMPEQAPKQRIQNFAEVALGYTKENAMMEAQRCLGCKKPPCVEGCPVN
                     VQIPQFIAKIKEGDFMSAIHIIKETNSLPAVCGRVCPQESQCESKCILGKKSEPVAIG
                     RLERFAADYQLGLGDVRVPTLPKKTGKKVAIIGAGPAGLTVAGELSKKGHECTIYEAL
                     HKAGGVLVYGIPEFRLPKAIVQREVDYLEKLGAKVRVDSIVGQTVTLDELFAQGYDAA
                     FIGTGAGLPYFLNIPGENLNGVYSANEFLTRANLMKAYLFPEYDTPIRVGSKVAVIGG
                     GNVAMDSARVSKRLGADVYLVYRRSREEMPARAEEAHHAEEEGIDFRLLTNPIRVVGD
                     DQGWVKGLECVKMELGEPDASGRRRPVEIKGSNFILDVDVVIVAIGQGPNPILTSTTP
                     DLELKKSGNIVADEETGQTSRTGVFAGGDIVTGAATVILAMGAGRKAAKAIDEYLQTK
                     K"
     gene            complement(2520..>3032)
                     /locus_tag="PKJ17_02560"
     CDS             complement(2520..>3032)
                     /locus_tag="PKJ17_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74880.1"
                     /translation="RQRQEVLGRVLSVLDAKEGTDLEEYSRLPETLKDIALNMDTYRE
                     LEGIIEDVMNKLLSRQEAKPVAKRWFWEKVIVQGIELINDFCHGEACPPDCRRIHSEA
                     PEAAAAILRLIYPDRFTAGPEETAAEIMGRYLQIRHPANDEDAFIRSLFSRYLEEDPE
                     TVGPSQDRVG"
     assembly_gap    3033..3132
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3133..3461)
                     /locus_tag="PKJ17_02565"
     CDS             complement(<3133..3461)
                     /locus_tag="PKJ17_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74881.1"
                     /translation="MKLAYTVAKGEQGTQRPMHAQRRAERFFRSRILKDLKDQFLEFM
                     NDGFSARLIDEKTKRLVDDLTGSGGVRLDRLGSIRFFVEQEDLFRDISETLRKLPQKD
                     RIADLLA"
     gene            3926..4237
                     /locus_tag="PKJ17_02570"
     CDS             3926..4237
                     /locus_tag="PKJ17_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74882.1"
                     /translation="MRDVIQKIVAAETEAKQAVEEARAEADRIIAEARKKAQDISATA
                     HKEAAAEADRIIATAAAEAGKEKEERLARAAARIEREVGIDEARRKLFVEGVVRCVCK
                     P"
     gene            4257..>5074
                     /locus_tag="PKJ17_02575"
     CDS             4257..>5074
                     /locus_tag="PKJ17_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOD74883.1"
                     /translation="MTGNLDYFVARLHGKRSSMAEGDRLDSLTRLRSLQEFTRAIFPE
                     REFQRTTDFQRQLIEELAREFSGFPGHLDRAQKHVVDWMPARLEAENIKVLLRACITR
                     INLEDVMERLVPLPGQTPAVVESLVRAPVLTDFVKLLPFGPLRKGLEKAIGDHGEDAR
                     PFFLEAALDSAYLEELIARLALLSNRDRQESEGVICQEADIFHLMLVLRGKFNYGLAA
                     DQLLSFHVPGARISRALLAAMLDDHDPWIAVGRAVMRAIDPLPVEGALSESSSA"
BASE COUNT         1047 a         1489 c         1514 g          924 t
ORIGIN      
        1 tggagaagaa agttcttgct ccctcgatca ccatgtacaa gctgtatgtc ccggacatcg
       61 ccagaaaggt gaaggccggc cagttcgtcg tcgtccgcgg cgacgacatg ggcgagcgca
      121 taccgctcac cgttgccgat tatgacacca agaagggcac catcaccatc atattccagg
      181 aagtgggcac atccacccag aagctcgcga agttcgaggc gggcatggcc ctcctcgacg
      241 tcgtcggccc cctgggtaaa ccgagccaca tcgagaagtt cggcaccgtt gtctgcgtgg
      301 gcggcggcgt cggcgtggcg cctgtcctgc ccatcgccaa ggccctcaaa gaggcaggca
      361 acgaggtcat ctccatcatc ggcgcccgca cgcagagcat gctcatcctc gaggaagaga
      421 tgaagaaggc gagcaccacc gtgcatgtca ccaccgatga cggcacatac ggacaccacg
      481 gttttgtcac cgacgtcctg aagaagataa tcgaagagcg cggcgctgcc aacatcgccc
      541 tcgtcgtggg tatcggcccc gtcatcatga tgaaggccgt caccgacgtg acccgcccct
      601 tcggcgtcaa gaccgtcgtc agcctcaata cgatcatggt cgacggcaca ggtatgtgcg
      661 gctgctgccg cgccaccatc gccggcgaga cgaagttcgt ctgcgtcgac ggtcccgagt
      721 tcgacgggca tgccgtggac ttccccgaac tggtcctgcg ccagaagatg tacaaccgcg
      781 aagagcggcg cgccctctgg gaccacaagt gcaagatcga cgcacaggaa aaggccgtca
      841 agaaggcccg caaacgcgtg aagatgccgg agcaggcacc gaagcagcgg atccagaatt
      901 ttgcggaagt ggccctggga tacacgaaag agaacgccat gatggaagcc cagcgctgcc
      961 tcggctgcaa gaagccgccc tgcgtggaag gctgccccgt caacgtacag atcccccagt
     1021 tcatcgcgaa gatcaaggaa ggcgacttca tgagcgccat ccatatcatt aaagaaacga
     1081 acagccttcc cgccgtctgc ggcagggtct gtccccagga aagccagtgt gagtcgaaat
     1141 gtatcctggg caagaagtct gaacctgttg ccatcggccg cctcgagcgc tttgccgcgg
     1201 actatcagct cggtctcggc gatgtccgcg ttcccacgct gccgaagaag acgggcaaga
     1261 aggtcgccat catcggtgcc ggccccgccg gtctcaccgt cgcaggcgag ctgagcaaga
     1321 agggccacga atgcaccatc tacgaagcgc ttcacaaggc gggcggcgtg ctcgtttacg
     1381 gcatccccga gttccgtctc cccaaggcga tcgtgcagcg cgaggtcgac tacctggaga
     1441 agctcggcgc caaggtgagg gtcgactcca tagtcggcca gacggtcacc ctcgacgagc
     1501 tcttcgccca gggttacgat gccgcattca tcggcaccgg cgcaggcctc ccctatttcc
     1561 tcaacatccc cggcgagaac ctgaacggcg tctactccgc caacgagttc ctgaccaggg
     1621 ccaatctcat gaaggcatac ctcttccctg aatacgacac acccatcagg gtcggctcga
     1681 aggtggcggt catcggcggc ggcaacgtgg ccatggactc cgccagggtc tcgaagcgcc
     1741 tcggcgcgga tgtgtatctc gtctatcgcc gcagccgcga ggaaatgcct gctcgtgcgg
     1801 aagaagcgca ccacgcggag gaagagggca tcgatttcag gctgctgacg aaccccatca
     1861 gggtcgtcgg cgacgatcag ggctgggtca aggggctcga gtgcgtaaag atggaactcg
     1921 gcgaacccga cgcgtcaggc agaagaagac ctgtcgagat caagggctcc aatttcatcc
     1981 tcgatgtgga cgtcgtcatc gtcgccatcg gccagggccc gaaccccatc ctcacctcca
     2041 ccactccgga cctggaactc aaaaaatccg ggaacatcgt ggcagatgag gaaacgggac
     2101 agacaagccg cacgggcgtc ttcgccggcg gcgacatcgt caccggagcc gctaccgtca
     2161 tcctggccat gggcgcggga agaaaagcag cgaaggcaat agacgaatat cttcagacga
     2221 agaagtaagg cttgccaatg aaagtgaaaa ggcggggtgt ggccaccccg cctttttttt
     2281 gccggtgaag aagggttcag gacccgggat gcggggtccg gaggcaagcc gggacgcgaa
     2341 ggtctttgaa ccacaaccgg catggcgata ggggtgatcc tgcggaaggg gtgacggtga
     2401 cgcggagtac gcgcagagaa tgatccggcg ggtcccctcg atcatctgtc tgcaaaacga
     2461 agcgtttcat gacacggtga ttctagcgtc tcgaaaagaa caggagagaa agaatgatat
     2521 cagccgaccc tgtcctgtga cggaccgact gtttcggggt cttcttcaag gtaacgggag
     2581 aagaggctcc tgatgaaggc atcctcgtca ttggctgggt gccgtatctg gaggtaacgc
     2641 cccattatct cggcggcggt ctcttcgggg cctgccgtga agcggtcggg gtagatgagc
     2701 ctcaagatgg cggccgccgc ctccggtgcc tcgctgtgga tgcggcggca gtcgggcgga
     2761 cacgcctcac cgtgacagaa gtcgttgatg agctcgatac cctggacgat gaccttctcc
     2821 cagaaccacc gcttcgcgac gggttttgcc tcctgccggg agaggagctt gttcatgacg
     2881 tcttcaatga tgccctcgag ctcccggtag gtgtccatgt taagggcgat gtctttcaaa
     2941 gtctcgggaa ggcgcgagta ctcctcgagg tcggtccctt cctttgcatc gagcacggaa
     3001 aggacgcggc cgagcacttc ctgtctctgg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnctggcgag caggtcggct atccggtctt tttggggaag ttttcgaagt
     3181 gtctccgaga tgtcgcggaa gaggtcctcc tgttcgacga aaaagcggat ggaaccgagc
     3241 ctgtcgagac gcacgccgcc gctgcccgtg aggtcgtcga cgaggcgctt cgtcttctcg
     3301 tctatgagcc gcgccgaaaa accgtcattc atgaattcga ggaactggtc cttgaggtcc
     3361 ttcagtatac gactgcggaa gaacctctcg gcgcgccgct gggcgtgcat cggccgctga
     3421 gtcccctgtt cgcccttcgc gacggtgtat gccagtttca tctgagtcat cttaaccccg
     3481 gcgacgcggt gtctgcccct ttttcctgct ttctctcgcc gatagttgac atttttatca
     3541 caaatttccc ccgttgaaaa gaccgttttg gatacccctg cccgaaattt caccacttgt
     3601 gtttatgaaa gttgacgcta tacttgatat atataaactt tcggtccggc cagacagtaa
     3661 ggcgctctta tcactcctgc atccgatcag cgcggtcaat gaggaaaggg tgatgagaaa
     3721 tgtgggggca cttaagctat attgaaatat gttcttccct gatggatgcc caccccgggg
     3781 cgcgccggca accgttgcct aggagccgaa gacccgtcaa ctcatccact gccgtcttct
     3841 caacgctcaa gcggctcaag cagttcaagc gatgtgaacc gttttcacgg ttcacatcat
     3901 tcaagccgtc tttccggagg tctctatgcg cgacgtgatc cagaagatcg tggcggccga
     3961 gacagaggcg aaacaggccg tcgaggaggc gagggccgag gccgaccgca tcatcgctga
     4021 agcccggaag aaggcgcagg atatcagtgc cactgcccat aaggaggccg ccgccgaggc
     4081 cgaccgcatc attgcgacag cggcagccga ggccgggaag gaaaaggagg aaagattggc
     4141 aagggcggcg gcgaggatcg aaagagaggt cggtatcgac gaggcccgaa gaaagctttt
     4201 tgtagaaggt gtcgtccggt gcgtatgcaa gccgtgacga cgcttcaagg aggccaatga
     4261 caggcaacct cgactacttc gtggcccgcc ttcacgggaa gcgcagctca atggctgagg
     4321 gagacaggct cgattccctc acgcgcctcc gcagccttca ggagttcacg cgggccatct
     4381 ttcccgagcg ggagtttcag aggaccacgg actttcagcg tcaattgatc gaggagcttg
     4441 ccagggaatt ctccggtttc cccggccatc tggaccgggc ccagaagcat gtggtcgatt
     4501 ggatgccggc ccgtcttgag gccgagaaca tcaaagttct cctcagagcg tgcattacca
     4561 ggatcaacct tgaagacgtc atggaacgtc ttgtgcccct gccggggcag acgccggcag
     4621 tcgtggaatc attggtgagg gcgccggttc tcaccgattt cgtgaagctt cttcccttcg
     4681 gaccgctgcg caagggtctg gagaaggcga tcggagacca tggcgaagac gcccggccct
     4741 tctttttgga agcggccctg gacagcgctt atcttgagga gctcatcgcg cgcctcgcgc
     4801 tcctctccaa ccgggaccga caggagtccg aaggcgttat atgccaggag gcggacatct
     4861 tccacctcat gctcgtcctg cgcggaaagt tcaactatgg gctggcagcg gaccagctgc
     4921 tttcctttca cgtccccggg gcccggatat cccgtgccct tcttgcggcc atgctggacg
     4981 accacgatcc ctggatcgcg gtggggcggg ccgtcatgcg cgccatcgat cccctgccgg
     5041 tggaaggggc cctgagcgag tcatcctcgg ccat
//
LOCUS       DAOPOA010000075         5043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_18108_length_5043_cov_3.888933, whole genome shotgun sequence.
ACCESSION   DAOPOA010000075 DAOPOA010000000
VERSION     DAOPOA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18108_length_5043_cov_3.888933"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKJ17_02580"
     CDS             <1..291
                     /locus_tag="PKJ17_02580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOD74884.1"
                     /translation="EGKGPVPKMSDLVTIHYKARLVDGTEFESSYWNNKPAEIAVSKA
                     IKGWTEALTKMPVGSIWRLYIPPDLAYGEVGAGMMIGPNAVIICDLELLAVR"
     gene            358..1377
                     /locus_tag="PKJ17_02585"
     CDS             358..1377
                     /locus_tag="PKJ17_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOD74885.1"
                     /translation="MVLEKICDLKTEREPLRLVDMPVPEPAGGEVLVSVRACGVCHTE
                     LDEIEGRTPPPRFPVVPGHQVIGSVEKCGPGTGHLQKGDRVGVAWIYSSCGTCGFCRD
                     GDENLCAEFVATGRDADGGYAEYMVVPEAFAFKVPDTFSDLEAAPLLCAGTIGYRSLR
                     LTGMRDGENIGLVGFGASAHLVIQMVRHMYPSSKIFVFARSAGERDFSRELGAAWAGD
                     TADSSPEKLHRAIDTTPVWGTVVHALGNLERGGRLVINAIRKEEIDKEELLALSYPEH
                     LWLEKEIKSVANVASRDVREFLELAASIPIRPEVEIFSLDEANRALLELKERKIRGAK
                     VLSIV"
     gene            1769..3172
                     /locus_tag="PKJ17_02590"
     CDS             1769..3172
                     /locus_tag="PKJ17_02590"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD74886.1"
                     /translation="MARVLIVDDDENFSALLSIMVKRAGHDAVAVATIKEGMKVLRGA
                     CEVVFLDIHLPDGNGLEILQDIRNTPSSPEIIIITGFEDSGAAELALKSGVWDYIRKP
                     SSIKQMQLALLRALQYRKEKGYQGLPAALKMDGIIGKSPLVGACLNLVAKAARGEANV
                     LITGETGTGKELFALSIHNNGPRSNKSFVVVDCASLPQNLVESTLFGHKKGSFTGADR
                     ARDGLIRQAHGGTLFLDEIGELPLSIQKSFLRVLQMRSFRPIGSDTEITSDFRLISAT
                     NRNLDRMVEEGQFREDLLFRIRTLQIDLPPLREHPEDIRELLVHYVARLCEQYGVGNK
                     GFSPEFLAALEAHDWPGNVREFISTLERAISEAFHQPTLYPKHLPAEIRIRIKRSELD
                     MGSGSEPHRETAEPVPVLRTYREEAIAAAEKKYLERVIKISEGRLREICRLSGLSRPR
                     LYALLKKYNLTRQTVPS"
     gene            3996..4391
                     /locus_tag="PKJ17_02595"
     CDS             3996..4391
                     /locus_tag="PKJ17_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HOD74887.1"
                     /translation="MSKLGPLFQMEDGKRERHIPVIDCPDRVAAGKPFAINVTIGNEV
                     KHPNTTEHHIKWVQVFFRPDNDRFSYQIAHCDFAAHGDSEAGSGKGPVVTDHRVSVVV
                     SVNGTGRIYALSLCNIHGLWESGKDVEVV"
     gene            4474..4938
                     /locus_tag="PKJ17_02600"
     CDS             4474..4938
                     /locus_tag="PKJ17_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD74888.1"
                     /translation="MEDVRRILVASRSTAECKSAFHYGVALARALVAEVSILHIEYDP
                     FEKIGFRYLGSLGPFQQEYLVRRKEIREEIEGLVRDEETAGMAIRQMIREGEPVGETV
                     RAVIENNIDLLVMAAHEEGRIERMIYGRSNHEIVRRLPCSILFVKDSKGGAE"
BASE COUNT         1140 a         1319 c         1596 g          988 t
ORIGIN      
        1 gaaggcaagg gccccgtgcc gaagatgagc gacctcgtaa ccatccatta caaggccagg
       61 ctcgtcgacg gaaccgagtt cgagagttcc tactggaaca acaagccggc ggagatcgcg
      121 gtgagcaagg cgatcaaggg atggaccgaa gcgttgacaa agatgcccgt gggctccata
      181 tggcgtctct atatccctcc ggatctggca tacggagagg tcggcgccgg catgatgata
      241 ggccccaacg ccgtcatcat ctgcgacctc gaacttctgg cggtgagata agaccgtcgc
      301 acgtcacagg ccacagaaaa aggcacaagc tgcaaggaga gggtagccgt gaaagcgatg
      361 gtgttggaga agatatgcga cctgaagacc gagagggagc cattgaggct cgtggacatg
      421 cctgtccccg agccggcggg cggtgaggtc ctggtcagcg ttcgcgcctg tggtgtctgt
      481 cacacggaac tcgatgagat cgaaggcagg acacctcctc ccaggtttcc cgtcgttccc
      541 ggacaccagg tcatcggatc ggtggagaag tgcggtcccg gcacggggca cctgcaaaag
      601 ggtgacaggg tgggagttgc ctggatctac tcgtcctgcg ggacctgtgg attctgccgc
      661 gatggtgacg agaacctttg cgcggagttt gtggccaccg gcagggacgc cgatggcggg
      721 tacgccgagt acatggtggt tcccgaggct ttcgcgttca aggtgcccga taccttcagc
      781 gacctcgagg ccgccccgct cttgtgtgcc gggacgatag ggtacaggtc gcttcgcctg
      841 acggggatgc gcgatggcga gaacatcggc cttgtcggtt tcggtgcttc ggcgcacctt
      901 gtgatacaga tggtgcgcca catgtatcct tcgtcgaaga tatttgtctt tgcccgcagt
      961 gccggggaga gggatttttc ccgggagctg ggtgccgcct gggcgggcga taccgcggac
     1021 agctcaccgg agaaactgca tcgcgccatc gacaccaccc ctgtctgggg taccgtggtg
     1081 cacgcgctcg gaaacctgga acggggaggg cgtctcgtca tcaacgctat acgaaaggag
     1141 gagatcgaca aggaagagct cctcgcgctc tcctatccgg agcacctctg gctcgaaaaa
     1201 gagataaaga gcgtcgccaa cgtggcttcc cgcgacgtcc gggaattcct tgaactggcg
     1261 gcatccatac ccatccggcc cgaggtggag atcttctctc tcgatgaggc gaacagggcg
     1321 cttcttgaac tcaaggaaag aaagatacgg ggcgccaaag tcctcagtat tgtctgaaaa
     1381 ggtcatcgct tgtctcagaa catgggaaac ccaagagaga gcgtggcggg tgttcggccc
     1441 gcgcttcggg aagggggagg ttgacaaaag tgaagggctt ttgatatcgt ttaattgtag
     1501 gatggagagc gcctgagggc gcagttttcg gcgtaagggg tgctgcaggt taccggggga
     1561 cgcagacgga agacccctga aaggccggcc acggatgaac gccggcctct cgatgaatac
     1621 ggagagataa ggggaaggat ggcagttccc gctcggactc atgtcgggag ttctgctgta
     1681 gatcacaggt ctgcgtcaga cgaagcatca cattcgtgaa ccggtaaggc ttgcggcaga
     1741 agcctgcttc accgggaggc gacgggcgat ggctcgggtt ctgatcgtag acgatgacga
     1801 gaacttctcc gcgctgcttt ccatcatggt caagcgggcc gggcacgatg ccgtggcggt
     1861 ggcgaccata aaggaaggca tgaaggtcct caggggagcc tgcgaggtgg tctttctcga
     1921 tattcatcta cccgacggga acggcctcga gatcctgcag gacatcagga acaccccctc
     1981 atcaccggag atcatcatta taacgggttt cgaagattca ggagctgctg agcttgccct
     2041 gaagagcggg gtatgggatt acataagaaa accgtcgtcc atcaagcaaa tgcagctcgc
     2101 gctgctcagg gccctgcagt acaggaagga aaagggatac cagggtcttc ccgccgccct
     2161 caaaatggac ggtatcatcg ggaagagccc gctggtcggt gcctgtctga accttgtcgc
     2221 caaggcggcc cggggtgagg cgaacgtcct catcaccggt gaaacgggca cggggaagga
     2281 actttttgcc ttatccatcc acaacaacgg cccccggtcc aataagagct ttgtcgttgt
     2341 ggattgcgcg tctcttccgc aaaacctcgt ggaaagcacg ctcttcggtc acaaaaaggg
     2401 ctccttcacg ggagcggata gagcgcggga tggcctcatc agacaggccc acgggggcac
     2461 gctgtttctt gatgagatcg gggagcttcc cctttccatc cagaagagtt tcctcagggt
     2521 cctgcagatg cggagctttc gccccatagg ctccgacacg gagatcacga gcgacttcag
     2581 gctgatctcg gccacgaaca ggaatttgga caggatggtc gaagaggggc agttccggga
     2641 ggacctcctt tttcggatac gtacactgca gatcgatctg ccgccgctgc gcgagcaccc
     2701 cgaagatata agggaacttc tcgtgcacta cgtcgcccgt ctgtgtgagc aatacggggt
     2761 gggcaacaag ggtttttcac cggaattcct tgcggccctc gaggcgcatg actggccggg
     2821 taacgtcagg gagttcatca gcacgttgga aagggctatt tcggaggcgt ttcaccagcc
     2881 tacgctctac ccgaaacacc tgcctgccga gatacggatc aggatcaaaa gatccgaact
     2941 cgacatgggc tccggctccg aaccccatcg cgaaaccgcg gaacccgttc cagttctcag
     3001 gacataccgt gaggaagcga tcgctgccgc tgagaaaaag tatcttgaac gggtgatcaa
     3061 gatctccgaa ggcaggctca gggagatctg ccggctttcg ggattatcgc gcccccgtct
     3121 ctacgcgctt ctgaagaaat acaatctcac aagacaaacg gtcccatcgt aaagaaggtt
     3181 caagggttca aaagttcatg agtataaggc agaactcaga aaaggcggtt cgtcagatgg
     3241 aattgaatgc gctgttcacg tgcgctggtc ggtctacaac cgacttcctt ccatggtcct
     3301 tcccttgaac ccttgaatga gtgtcttgcg cggcgagaca cagtactttt tggcaagaca
     3361 tcatgttgca gcacgagaca aaagctttcc gtaatgagat ccgcagttgg ggtgttactc
     3421 cttgaaagat aaggctaata cgcgatcttc tctgcgtggt cataccgtgg catgcttttt
     3481 gatataactg taggagggtt gccgaacgcg gcaatgactt gccagggagt gagctcgcgg
     3541 aaaggcgggc atatttacct ccctagaaca tacgttgaag tagagggggc taccggctga
     3601 cagctttgac tcctgaatag aaccgaaaga ctggggtctc acggggatcg cggcgaagtt
     3661 ccgaacgcat ctgcccggga ttggtcgccg atgggaccga cgtcgagccc ggcaaaaccg
     3721 ctaacaaggg gtcatgacgg acatcgtgta aaccggaagg gggcctttct gaatccacgg
     3781 atgtttagca ccctggcgtg cctgctacag acacgcgtcg tatccggatc gccaatggca
     3841 tatcttgcgc gggtcctcgc ccgactaacg gctgcgacga atcacttcct gttttcatcc
     3901 ctggggacaa catgaagaag gcccgtatag ctgccgaatg gaggcgaccg aaggcgactg
     3961 aaggagtggt gagctttgcg cgaggcgcgg ggttgatgag caaattgggt ccattgtttc
     4021 agatggagga cggcaagagg gaaagacaca tcccggtgat cgattgcccc gacagggtag
     4081 ctgccggcaa gccgtttgcc atcaatgtga cgatcggcaa cgaggtcaag catccgaaca
     4141 cgacggagca tcacatcaag tgggtccagg tcttcttcag gcccgataac gacaggtttt
     4201 cctaccagat agcgcactgc gatttcgccg cccacggtga ttccgaggcg ggctccggca
     4261 agggccctgt cgtgaccgat cacagggtca gcgtggtcgt gtcggtgaac ggcacgggaa
     4321 ggatctacgc cctgagcctg tgcaacatcc acggtctgtg ggaaagcggc aaagacgtgg
     4381 aggttgtcta gagatctaca accggtcgac ggatgcgaca gtattttcct gccggtattg
     4441 tggacggaag ggccgctgaa aaggaggagt tgcatggagg acgtgaggcg tattcttgtg
     4501 gcaagcagat ccaccgcgga gtgcaagagc gcgtttcatt acggcgttgc gctggccagg
     4561 gccctcgtgg cagaggtctc catactgcat atcgaatacg atccatttga gaagatcgga
     4621 tttcgatacc ttggcagcct tggtcctttt cagcaggaat atctcgttcg ccgcaaggag
     4681 atacgcgagg agatagaggg ccttgtacgt gatgaagaaa cggcgggaat ggccatcagg
     4741 cagatgatcc gggaggggga accggtcggg gagaccgtgc gggcggtgat agagaacaac
     4801 atagatctcc ttgtcatggc cgcccatgag gaaggccgga tagaacgaat gatatacggc
     4861 agaagcaatc acgagatcgt cagaagactt ccctgttcga tcctgttcgt taaggattcg
     4921 aagggaggcg cggaatagcg tgcggggatc atcgagcagc gccgatgagt ccctgccgca
     4981 tccaggtcct gcgggaaagc ggcaagggcc tgcagatgtc ccgtgaggga gttaaagttt
     5041 cgg
//
LOCUS       DAOPOA010000076         5005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_18269_length_5005_cov_3.355354, whole genome shotgun sequence.
ACCESSION   DAOPOA010000076 DAOPOA010000000
VERSION     DAOPOA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18269_length_5005_cov_3.355354"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKJ17_02605"
     CDS             <1..492
                     /locus_tag="PKJ17_02605"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOD74889.1"
                     /translation="AVSDTIEGLAGVTATLKWPNDVLVRGRKISGTLIELSTEADMVR
                     FVVIGIGLNVNMGSEDMDRDIRATATSLLMETKKTYERPKVCGILLSNLERYYDLFKK
                     EGARAICDIWEERAQIRGKYLTINQMGEVYAGTAKGIDRDGAILLDIEGTTKKILAGD
                     VNF"
     gene            492..1274
                     /locus_tag="PKJ17_02610"
     CDS             492..1274
                     /locus_tag="PKJ17_02610"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOD74890.1"
                     /translation="MLLVIDIGNTNIVFGVYHDGKLLNHWRLSTALGKTVDEYAILLN
                     SLLYIEKIRLNEIHSAIVSCVVPPLLIPFEIVCRKYIGIMPIVVEPGIKTGMPVLYEN
                     ATELGADRIVNAVAGYDKYKKALIIVDFGTATTFEYITPKGEYAGGSIAPGILISLDA
                     LFERASKLPRVELVQPKTLISKNTVQAMQAGITYGYVSLVDGIVKRMKEEVRTDPLVI
                     ATGGLSSLIYKGSETIDVVDEFLTLEGLKILYEKNKDHHKFK"
     gene            complement(1454..2164)
                     /locus_tag="PKJ17_02615"
     CDS             complement(1454..2164)
                     /locus_tag="PKJ17_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOD74891.1"
                     /translation="MNYVLAVLAVGFIVLIHETGHFIAAKLAGIPIRTFSVGFGPRLY
                     ALKKGGTEYRLSLIPLGGYVMPDIDDEKAFFDLPVLRRVVLAAGGPAASMVLPLFCFA
                     LSDALRFGPGPGSLLFQPLEQTATAFIRIASIIPLLFTHHGELSGIAGIVSQGGRFIG
                     TDGHNLLSFTALMSINLAVLNLLPVPVLDGGKIVMYAMEKLSRKVVKLHYALSVAGWA
                     LMLAIMIYATVLDVGRMM"
     gene            2321..2989
                     /locus_tag="PKJ17_02620"
     CDS             2321..2989
                     /locus_tag="PKJ17_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOD74892.1"
                     /translation="MIEERDDGRNFREVDAMRRGMKRTGLLAATLAFALSAGCVVSVG
                     TGSGSDGLVPVPMPSGPYVVTQGRRLVVAVGPVNVPGYITRAATIMDSTMNSANISSA
                     DWKASVLEWQIPQVVTENVRRLFAPYGIEVAPHPQGRNADYRIAVDLRVFEVSTFDVL
                     ETRGRWVLYGPGGTSPMMERDIAFSTHLHEPGDAGTKDAMSRALADLTRGIVKDCGGV
                     LGIR"
     gene            3293..3478
                     /locus_tag="PKJ17_02625"
     CDS             3293..3478
                     /locus_tag="PKJ17_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74893.1"
                     /translation="MTVRLICAKYLQGQCRDENPEACEHHAPHRPSVVCNDPLIVCDD
                     GSPTRCIPILDDIDNLE"
     gene            3559..4938
                     /locus_tag="PKJ17_02630"
     CDS             3559..4938
                     /locus_tag="PKJ17_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD74894.1"
                     /translation="MDATASLKRNTLLAAVMSSFLGPFTASSVTVALPRIGTHFAMDA
                     ISLGWVATAYLLAAAAFLVPVGRASDIYGRKKIFLWGMVFINLSSLLAGFSSSGTELI
                     LWRIVQGIGSSMIFGTGVTIVSSVYPASERGKALGIVLTAVYVGVSSGPFIGGVLTDL
                     LGWRSLFFTNVAIGMVVIVTILWKIKEEWVEAKGESFDMTGSILYVVAFTAAMYGLTA
                     LPSAKGISFFSGGLVGLFLFGVWELKIEHPVLEMRLFMKSRVFLFSNLAALINYSATF
                     AVTFLISLYLQYIKRLSPQTAGLVLISQPLMQAVLSTFTGRLSDRVEPRVVSSAGMVS
                     LMIALLLFGFLDEGTRTWLVVLNLLFLGFGFALFVSPNTNAIMSSVDKKFYGVASGTM
                     ATMRVIGQTLSMGIVMFVFTLFMGRIQITEQYYGQFLRSTKTTFFIFAFLSFLGIFAS
                     LARGRIHNR"
BASE COUNT         1074 a         1388 c         1419 g         1124 t
ORIGIN      
        1 gcagtatcgg acaccatcga gggactcgcg ggtgtgacgg caactctcaa atggcccaac
       61 gatgtgctcg tgcggggaag gaagatatcg gggacactca tcgagctctc caccgaggcg
      121 gacatggtgc gcttcgtcgt catcgggatc ggcctcaacg tcaacatggg ctccgaggac
      181 atggacaggg atatcagggc gacggcgacc tcactgctca tggaaacaaa aaaaacatat
      241 gaaaggccga aggtgtgtgg tatccttttg tccaatcttg aaagatatta cgatcttttc
      301 aagaaagagg gtgcccgggc catctgcgat atctgggaag aaagggccca gatccgtggg
      361 aagtacctca cgataaacca gatgggcgag gtctacgccg ggacggcgaa aggcatcgac
      421 cgggatggtg cgatccttct cgacatagag ggcaccacga agaaaatcct tgcaggagat
      481 gtgaacttct aatgctgctt gtcatcgata tcgggaatac caacatcgtt tttggcgtct
      541 atcacgacgg caagctgctc aaccactgga ggctgtccac ggccctgggc aagacggtcg
      601 acgaatacgc catcctcctc aacagccttc tctacataga gaagatacgc ctgaacgaga
      661 tacacagcgc catcgtgtcc tgtgttgtgc cgccgctcct gatccccttt gagatcgtgt
      721 gcaggaaata catcggcatc atgcccatcg ttgtcgagcc cggcatcaag acgggaatgc
      781 ccgtacttta cgagaacgcc acggaactcg gtgccgacag gatcgtcaac gccgtggccg
      841 ggtatgacaa gtacaagaag gcgctcatca tagtcgattt cggcaccgcc accaccttcg
      901 aatacatcac ccccaaaggg gagtacgcgg ggggttccat cgccccgggt atcctcatct
      961 ccctcgacgc cctcttcgag cgggcatcca aacttccgcg cgtcgagctc gtccagccga
     1021 agacgctcat cagcaagaac accgtccagg cgatgcaggc cggcatcacc tacggctatg
     1081 tgagcctcgt cgacgggatc gtcaagagga tgaaggaaga ggtcaggacc gatcccctgg
     1141 tcatcgcgac gggcggtctc tcaagcctaa tctacaaggg ttccgaaacg atcgacgtcg
     1201 tcgacgagtt cctcacgctg gagggactga agatcctcta cgagaagaac aaggatcatc
     1261 acaaattcaa ataagtgaaa aaccgtttta agttttaagt tttaggtttt aagtgaaaag
     1321 aaaaggcccc acgggggcct tcctgcgttt cactcactat cttcccttgc cggagacatt
     1381 gcctccggca ctgtctttga cttaaaactt aagacttaaa acctaaaact taaaacttaa
     1441 aacggtcctt cacttacatc atcctcccca catccagcac ggtcgcgtag atcatgatgg
     1501 cgagcatgag ggcccagccg gctacggaga gggcatagtg gagcttcacc accttgcggc
     1561 tcagcttttc catggcgtac atgacgatct ttccgccgtc gaggacgggc acgggaagga
     1621 ggttgaggac ggcgaggttg atgctcatga gggccgtgaa ggacaggagg ttgtggccgt
     1681 ccgtcccgat gaagcggccg ccctgggata cgatcccggc aattcccgaa agctccccat
     1741 ggtgggtaaa aaggagggga atgatggagg cgatcctgat gaaggcggtg gccgtctgtt
     1801 ccaacggctg aaaaagaagg cttccgggac cgggtccgaa acgcagcgcg tctgacagcg
     1861 cgaaacagaa caagggcagg accatgctgg ccgcggggcc gccggcggcg aggactaccc
     1921 gtcgaaggac aggcaggtcg aagaacgcct tctcgtcgtc gatgtccggc atgacatatc
     1981 cgccgagggg aatgagggac agtctgtatt ccgtaccgcc tttcttcagc gcatagagtc
     2041 tcgggccgaa gccgacggaa aaggtccgga taggtatccc tgccagcttt gcggcgataa
     2101 aatggcctgt ttcatggatg agaacgataa acccgacggc aagcacggca agcacatagt
     2161 tcattgtctg cgctcctctt gtcttaagac cggtctcccg ggagaccttt cggcattttc
     2221 cgtattctat atcattatcc acccctctgg caaaagcata aaaagggggt ctcacgttac
     2281 aggagttcct ttcattgttt gcgcaaagga tatagaattc atgatagaag aaagagatga
     2341 cgggcggaac ttcagggagg ttgacgcaat gagacgtgga atgaagagga caggactgct
     2401 tgcggcaact ctcgcgttcg cgctgtcggc cggttgcgtg gtttccgtgg ggacgggttc
     2461 ggggtctgac ggtcttgtcc ccgtgccgat gccttccggc ccttacgtgg tgacacaggg
     2521 aagaagactt gtcgtggccg taggccccgt gaatgtcccc ggatatataa cccgtgccgc
     2581 cacgatcatg gattccacca tgaactcggc gaacatctca tcggcggact ggaaggcgag
     2641 cgtgctggaa tggcagatcc cgcaggtggt taccgagaac gtgaggcgcc tcttcgctcc
     2701 ttacggaatc gaagtggccc cgcatccgca ggggaggaat gccgattacc ggatagcggt
     2761 ggacctgagg gtcttcgagg tctcgacgtt cgacgttctg gagacgcggg gaagatgggt
     2821 cctctatggc ccgggcggta cgtctccgat gatggagagg gatatcgctt tttcgacaca
     2881 ccttcacgag cctggcgacg cgggaacgaa ggatgcgatg agccgcgctc ttgccgatct
     2941 cacgaggggg atcgtcaagg actgtggagg cgtcctcggg atacgatgaa gggccgacgc
     3001 cccccctggt tgaggttatt tctctgcctt ttccgtctta ttggtcttct ttcccttagg
     3061 ccgcgtcttg ccggaagagc cgcgccagat ctttcctctc ttggtccgtt tgtcaccttt
     3121 gcccatggat actgacccct ttctcaacag gttatgtatt tgtttcgaac gcaaacttag
     3181 cgaagaatcg gggaaaagtc aactgttttt cggatgttcc cgcagcgtcc ttcgaaaagg
     3241 cgacaggtct tggcaaagga aatgatctgc cgtctgtggt agaatgacgt tcatgacagt
     3301 ccggctcatc tgtgccaagt atctgcaggg tcaatgcagg gacgaaaacc ctgaggcatg
     3361 cgaacatcat gcgccccaca ggccttccgt ggtctgcaat gaccccctca tcgtgtgtga
     3421 cgacggatct cccaccaggt gcatcccgat ccttgacgat atcgacaatc ttgagtaggg
     3481 aacgggcgga cccgtcggcg aaaagtttct ctctgtcggg gacttctgtg gtatatatcg
     3541 tcaggtcgac aacactccat ggacgctacg gcaagcctca agagaaacac tctcctcgcc
     3601 gcggtaatga gctcctttct cggacctttc accgcttcct ccgttaccgt tgcccttcca
     3661 cggataggga cacactttgc aatggacgcc atctcgctgg gctgggtggc gacggcatat
     3721 ctgcttgcgg ccgcggcctt tctggtcccg gtgggaaggg cttcggacat atacggcagg
     3781 aagaagatct tcctctgggg aatggtcttc ataaacctct cttcccttct tgccggtttt
     3841 tccagttcgg ggaccgagtt gatcctctgg aggatcgtcc agggcatcgg cagttcgatg
     3901 atattcggga cgggcgtgac catcgtctcc tctgtctacc ccgcgagcga acgtggcaag
     3961 gccctcggca tcgttctgac ggctgtctac gtaggtgttt cttcggggcc tttcatcggg
     4021 ggcgtcctca cggaccttct cggctggaga agccttttct tcacgaacgt cgccataggc
     4081 atggtcgtca tcgtgaccat actctggaag atcaaagagg agtgggtcga ggcgaagggc
     4141 gaaagtttcg acatgacggg ttccatcctt tacgtcgtgg ccttcacggc cgccatgtac
     4201 ggtctgaccg ccctcccgtc ggcgaagggg atctccttct tttccggtgg tcttgtgggg
     4261 ctttttctct ttggcgtatg ggagctcaag atcgagcacc ccgttctgga aatgcgcctt
     4321 ttcatgaaaa gcagggtctt tctcttttcc aacctcgctg ccctgatcaa ctacagtgcc
     4381 acctttgccg tcacctttct catcagcctc tacctgcagt acatcaaaag gctcagcccc
     4441 cagacggcag gccttgtcct tatctcacag ccgctcatgc aggccgtcct gtcgaccttc
     4501 acgggaagat tgtcggaccg tgtggagccc cgtgtcgtgt cctccgcggg aatggtctcc
     4561 ctcatgatcg ccctccttct tttcggtttc cttgatgaag gcacccggac ctggcttgtc
     4621 gtcctcaacc ttcttttcct ggggttcggt ttcgcccttt ttgtctctcc caacacgaac
     4681 gccatcatga gctccgtgga caagaagttc tatggagtgg cctcgggcac catggcgacg
     4741 atgcgcgtca tagggcagac actgagcatg ggcatcgtca tgttcgtttt cacgcttttc
     4801 atggggagga tccagataac ggagcaatac tacgggcaat tcctgcgaag cacgaagacg
     4861 accttcttca tcttcgcctt cctgtccttc ctcggcatct tcgcgtcgct ggcaaggggg
     4921 aggatacata acagatgacc gcttgagggt ttgaatggct tgaatcgctt gacaggctgt
     4981 tgataaaccc attttctacg tcatt
//
LOCUS       DAOPOA010000077         5004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_18274_length_5004_cov_3.089715, whole genome shotgun sequence.
ACCESSION   DAOPOA010000077 DAOPOA010000000
VERSION     DAOPOA010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18274_length_5004_cov_3.089715"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKJ17_02635"
     CDS             <1..293
                     /locus_tag="PKJ17_02635"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOD74895.1"
                     /translation="GVSRVDIFFPENHSLKDKRQMLRKVIEKTRAKFNISMIEVDQSN
                     LWQRARIGFAVAGVRQDHVSKVIENVHEYMESLYLGEVIDTSSEIIVIGDEI"
     gene            290..640
                     /gene="rbfA"
                     /locus_tag="PKJ17_02640"
     CDS             290..640
                     /gene="rbfA"
                     /locus_tag="PKJ17_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOD74896.1"
                     /translation="MRYRRLRVQDLLREEISLIIQRELQDQGLGFITVVEVKMSEDLK
                     SAKVYVSIYGSEEEQKHTFEALKRSKGYIKFLLGKRVQLRYMPEITFLLDDTLERVQR
                     MEEIFRKEADEGKG"
     gene            624..1586
                     /locus_tag="PKJ17_02645"
     CDS             624..1586
                     /locus_tag="PKJ17_02645"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOD74897.1"
                     /translation="MKAKVRRLIEEGCSFLVTTHIDPDGDAIGSVFAFAMALGTLGKE
                     ATVYLRDRIPYRYEFLPAPGRVVHAVPEGKYDAVFVLDCGSLFRVGAGHDRIGAMAPI
                     VNIDHHDTNDHFGSVNLVDPGASSTGEIIYRLFRSLKVKVDPDMAVNLYTAIFTDTGS
                     LRYDNTSLEAFRICEEMVKAGVRPSWVAGMVYENHPKERFLLLGEVLCTLTTYDKKIA
                     VARVTSDMFERTGTNREFTDGFVEFIKEIRGVEVAVLIREIDEHHHKVSMRGKGAVDV
                     AAVCNSFGGGGHRNAAGCRIEGTMEETEEKLLRTLAPEGWVKDE"
     gene            1647..2531
                     /gene="truB"
                     /locus_tag="PKJ17_02650"
     CDS             1647..2531
                     /gene="truB"
                     /locus_tag="PKJ17_02650"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOD74898.1"
                     /translation="MNGYLIIDKKAGMSSYDVIRRLKRLHKFKKIGYIGTLDRNATGI
                     LPVALDEGVKLIPFLENVEKQYRARFLLGVTTETQDIEGKVLTETPTEPFDRQVIEDA
                     LESYLGRITQRVPAYSSKKVNRKPLYKWARSGVQVEQPVKEVEIFDITLLDYTHPHLD
                     VEVTCSKGTYIRALASDLGEKLGCGATLFTLKRTRHGEFSEGMGTDIDCFKMEQDLLN
                     YLLPLENILGSVRGMVVETALEKFLRHGMPVPIAGSAREWRHGEAARLFNRQGALIAV
                     GIADLGSKTIKIKRLINY"
     gene            2542..2811
                     /gene="rpsO"
                     /locus_tag="PKJ17_02655"
     CDS             2542..2811
                     /gene="rpsO"
                     /locus_tag="PKJ17_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOD74899.1"
                     /translation="MPLDTQRKKSIIEDFKTHDTDTGSPEVQIALLTERIRSLTEHFK
                     KFSKDHNSRRGLLVLVGSRRRLLNYLKDKNVDRYKKVVEKLGLRK"
     gene            2830..4908
                     /gene="pnp"
                     /locus_tag="PKJ17_02660"
     CDS             2830..4908
                     /gene="pnp"
                     /locus_tag="PKJ17_02660"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOD74900.1"
                     /translation="MEENITIEFAGRPLTISAGGLAKQADGSVMARYGDTVVLVTAVA
                     EKQARDKDFLPLTVNYQEMSYAAGKFPGGFFKREGRPTTREILTSRLIDRPLRPLFPK
                     NWRYETQVISTVLSADQDNDPGVLSLVGASCAVTISDIPHDGPFAGVRVGRIGGEFVV
                     NPTFPQMADSDIDIIVTATRDAIIMVEGEAHFVKGTDLIEAIEFGHQAMMPLIEMQER
                     LREKIGKQKRQIPVPENLDEKKKEVRAIVEKDLIESFAVRAKLERYERQDVILDDLKA
                     RYSEEEAGIISKVYEDVTRDLMREQLLSTGRRIDGRATDQIRPISCVVGALPRTHGSA
                     VFTRGETQALAVTTFGTSDDEQKIESLHEGETYKTFLLHYNFTPFSVGEVSMLRGPTR
                     REIGHGNLAERALTPILPEREAFPYTIRIVSEILESNGSSSMATVCGGCLSLMDAGVP
                     IKEPVAGIAMGLVKEGDRELILSDILGDEDHLGDMDFKIAGTREGVTAIQMDIKIKGI
                     SKDTMQKAVIQGQQGVGTILDIMTATIAGPRENLSPYAPRIYTMTIKPDKIREVIGPG
                     GKVIRSIIERTGVKIDIDDSGIVNIVSVDEESANAAIEIVKSIVKDVEVGAVYEGKVR
                     RVLDAGVIVELGPNLDGFCHVSQLDDKFVKKASDVAREGDDITVKVIGVEDNGRIKLS
                     RKAVLKDQRG"
BASE COUNT         1198 a         1328 c         1572 g          906 t
ORIGIN      
        1 ttggtgtatc ccgcgtcgac attttctttc ctgagaatca ctctctcaaa gacaagcggc
       61 agatgctgag gaaagtgatc gagaagacac gggcgaaatt caacatctca atgatagagg
      121 tggaccagtc gaacctgtgg cagcgcgcca ggatcggttt cgccgtcgcg ggcgtgaggc
      181 aggatcacgt gagcaaggtc atagagaacg tccatgagta tatggaatct ctgtatctgg
      241 gcgaggtgat cgatacttcg tcggagataa tcgttatcgg ggatgagata tgagataccg
      301 gcgcctgagg gtccaggacc tgctccgcga agagatatcg cttatcatcc agcgggagct
      361 gcaggaccag ggtctcggct tcatcaccgt cgtcgaggtg aagatgagcg aggacctgaa
      421 gtccgccaag gtctacgttt ccatatacgg gagcgaagag gagcagaagc acaccttcga
      481 ggccttgaag cgttccaagg gttacataaa gttcctcctt ggcaagaggg tgcagttgag
      541 gtacatgccg gagatcacct ttctcctcga tgacaccctg gaaagggtgc agaggatgga
      601 ggagatattc aggaaggagg ccgatgaagg caaaggttag gaggctcatc gaagagggtt
      661 gcagtttcct cgtgacgacc cacatcgatc cggacggaga tgccatcggg tccgttttcg
      721 cctttgccat ggccctgggc acgctcggca aggaggccac ggtctacctc agggacagga
      781 tcccgtacag gtacgagttt ctcccggcgc cgggcagggt ggtccatgcg gtacccgaag
      841 gcaagtacga cgccgtcttc gtcctcgact gcggcagcct tttccgggtc ggggcggggc
      901 acgaccggat cggcgcgatg gcaccgatcg tcaatatcga ccatcacgac acgaacgacc
      961 acttcgggtc tgtgaacctt gtcgatcccg gggcgtcgtc gacgggagag atcatttacc
     1021 ggctcttcag gtccctgaag gtcaaggtgg accccgacat ggccgtcaac ctctatacgg
     1081 ccatcttcac cgataccggt tcgcttcggt acgacaacac gagcctcgag gccttcagga
     1141 tatgcgaaga gatggtgaag gcgggcgtga ggccgtcatg ggtggcgggg atggtctacg
     1201 agaaccaccc caaggaaagg ttcctactcc tcggtgaggt cctgtgcacg ctcaccacgt
     1261 acgacaagaa gatagcggtg gcccgcgtga cgtcggacat gttcgagagg acggggacga
     1321 accgcgagtt caccgatggt tttgtcgagt tcataaagga gatacgcggt gtcgaggtcg
     1381 cggtgctgat acgggagatc gacgagcacc accacaaggt cagcatgcgg ggcaagggcg
     1441 ccgtggacgt tgccgccgtc tgcaattcct tcggcggcgg ggggcatcgc aatgccgccg
     1501 ggtgccgcat cgagggcacc atggaggaaa cggaagagaa actccttcgc accctcgcgc
     1561 ccgaaggctg ggtgaaggac gaatagaaga ccggtgccgc ccgaattggg cggcgggacg
     1621 aagaaggaag gaaacggata cagggaatga acggatactt gataatcgac aagaaggcgg
     1681 ggatgtcctc ctacgatgtg atacgacggc tgaagaggct ccacaagttc aagaaaatag
     1741 ggtacatcgg caccctcgac cgcaacgcga cgggcatcct gcctgtcgcc ctcgatgaag
     1801 gggtcaaact catacctttc ctcgagaacg tagagaagca gtaccgcgcg agattcctcc
     1861 tcggcgtgac gacggagacc caggacatcg agggcaaggt gctcacggaa acgccgacgg
     1921 agcctttcga ccggcaggtc atcgaggatg cgctcgagtc ttacctgggc aggataaccc
     1981 agcgggttcc cgcttattcc tcgaaaaagg tcaaccgcaa acccctctac aagtgggcgc
     2041 gaagcggagt gcaggtggag cagcccgtca aggaggtgga gatcttcgat atcacccttc
     2101 tcgattacac ccaccctcat ctcgatgtgg aggtcacctg ttccaagggt acctacatca
     2161 gggcgctggc gagcgacctc ggcgagaagc tcgggtgcgg ggccacgctc ttcactttga
     2221 aacggacgag acacggagag ttctcggagg gcatgggaac tgatattgac tgtttcaaaa
     2281 tggagcaaga cctgttaaac tatttgttac ctttggaaaa tatcctgggt tccgtgcggg
     2341 gcatggtggt ggagacggcc ctggagaagt tcctgagaca cgggatgccc gtgccgatag
     2401 cgggcagcgc gagggagtgg aggcacggcg aggccgcgcg cctcttcaac aggcaggggg
     2461 cgctgatcgc cgtgggtatc gcggacctgg ggtccaaaac gataaagata aaacgattga
     2521 tcaattatta aggaggaata tatgccactg gatactcaga ggaaaaagtc gatcattgag
     2581 gatttcaaga cccatgatac cgacaccggt tccccggaag tgcagatagc gcttctcacg
     2641 gagcggatac gatccctgac ggagcatttc aagaagttct ccaaggacca caactcgaga
     2701 agagggcttc tcgtccttgt cggaagcaga agacggcttc tcaactatct caaggacaag
     2761 aacgttgacc gctacaagaa ggtcgtggag aagctcggtc ttagaaaata aaggaataat
     2821 aggaaaaata tggaagaaaa cataactatc gaattcgcgg gccgaccgct gactatcagc
     2881 gccgggggtt tggcgaaaca ggccgatgga agcgtcatgg cgcgctacgg ggacacggtt
     2941 gtcctcgtca ccgccgttgc cgaaaaacag gcgagggaca aggactttct gccgctcacc
     3001 gtcaactatc aggagatgtc ttacgccgcc gggaagttcc ccgggggttt cttcaagagg
     3061 gaaggacgtc cgaccacccg ggagatactc acatcgcgcc tcatcgacag gccgttgagg
     3121 cctctgttcc cgaagaactg gcgatacgag acgcaggtga tctcgacggt gctttccgcc
     3181 gaccaggaca acgacccagg cgtcctgagc ctcgtggggg cgtcctgcgc ggtcaccata
     3241 tcggatatcc ctcatgacgg gcctttcgcg ggtgtcaggg tgggaaggat aggcggtgag
     3301 ttcgtcgtga acccgacctt tccccagatg gccgacagcg atatcgatat catcgtcacg
     3361 gcaacccgcg acgccatcat catggtggag ggcgaggcgc atttcgtgaa aggcaccgat
     3421 ctcatcgagg ccatcgagtt cggccaccag gccatgatgc ctctcatcga gatgcaggag
     3481 aggctccgcg aaaagatcgg aaagcagaag aggcagatac cggtccccga gaacctcgac
     3541 gagaagaaga aagaggttcg cgccatcgtg gagaaagacc tcatcgaaag ctttgccgtc
     3601 agggcgaaac tggaacggta cgaacgccag gacgtgatac tggacgacct caaggcccgt
     3661 tattccgagg aggaggcggg gatcatctcc aaggtttacg aagacgtgac acgggacctc
     3721 atgcgggagc agctcctgtc gacgggcagg cgcatcgacg ggcgggccac ggaccagatc
     3781 cggcccatct cgtgcgttgt cggcgccctt cccaggacgc acggctcagc ggtcttcacc
     3841 cggggtgaga cgcaggcgct ggcggtaacc accttcggga cgtccgatga cgagcagaag
     3901 atcgagtccc tccacgaggg agaaacgtac aagaccttcc tgctccatta caacttcacg
     3961 cctttttccg tcggtgaggt ctcgatgctg cggggaccga cacggcgcga gataggccac
     4021 ggcaatctcg ccgagagggc gctgacaccg atcctgcccg aaagggaagc attcccctac
     4081 acgatcagga tcgtctcgga gatcctcgaa tcgaacggtt cctcgtcgat ggccacggta
     4141 tgcggcggct gcctttcgct catggacgcc ggtgtgccca tcaaggagcc cgtcgccggc
     4201 atcgcgatgg gcctcgtgaa agagggcgac cgggagctca tactttcaga catcctcggc
     4261 gacgaggacc acctgggcga tatggatttc aagatcgccg gtacgcggga aggcgtcacg
     4321 gctatccaga tggatatcaa gataaaaggg atatcgaagg acaccatgca gaaggcggtc
     4381 attcaaggcc aacagggtgt cggcacgatt ctcgacatca tgacggcgac cattgcgggg
     4441 ccgagggaga acctttcgcc ctacgcaccc aggatctaca cgatgacgat caagcccgac
     4501 aagatacggg aggtcatagg cccgggtgga aaggtcatca ggagcatcat cgagagaacg
     4561 ggtgtcaaga tagacatcga cgactccggg atcgtcaata tcgtttccgt ggacgaggag
     4621 tccgccaacg ccgccatcga gatcgtcaag tccatcgtga aagacgtcga ggtgggtgcc
     4681 gtttatgaag gcaaggtgcg gcgggtgctc gatgcaggtg tcatcgtgga gctcgggccc
     4741 aacctggacg gtttctgcca cgtgagccaa ctcgatgaca agttcgtcaa gaaggcttcc
     4801 gatgtggcca gggaaggcga cgatatcacc gtcaaggtca tcggcgtgga agacaacggg
     4861 aggataaaac tgtcgagaaa ggctgtcctc aaggaccaga ggggatagca ttaattagat
     4921 gtataagaag accgtactcg ataacggtat caccgtcgtt acagaatcca tagcatacta
     4981 ttcgacggtt tcgataggta tgtg
//
LOCUS       DAOPOA010000078         4960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_18501_length_4960_cov_3.743119, whole genome shotgun sequence.
ACCESSION   DAOPOA010000078 DAOPOA010000000
VERSION     DAOPOA010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18501_length_4960_cov_3.743119"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PKJ17_02665"
     CDS             complement(<1..930)
                     /locus_tag="PKJ17_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOD74901.1"
                     /translation="MERKNILCIGAGYVGGPTMAMIAYKCPQYRVTVVDINHEKIERW
                     NSDDLPVYEPGLDEIIRATRGKNLFFETDIDKGIRESEIIFVGVNTPTKTFGAGAGKA
                     ADLQYWEKTARQIREASESPKIVVEKCTIPVKTALAMERILTSKDGDISFDVLSNPEF
                     LAEGTAMRDLEFPDRVLIGSRETPSGIEARKAVVDIYLNWVPRERIIESNIWSSELSK
                     LVANAFLAQRISSINAVSALCEKTEADVAEVSRAIGLDSRLGPKFLNASVGFGGSCFK
                     KDILNLAYICRSYGLEDVADYWESVVDINEYQKE"
     gene            complement(1022..2815)
                     /locus_tag="PKJ17_02670"
     CDS             complement(1022..2815)
                     /locus_tag="PKJ17_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD74902.1"
                     /translation="MKDGKKTKDQLIEELLSLRRRVADLEGSDAGGRSRPEVPSGLEE
                     RYRTLFEHASDAIIVADPEGNFLEVNRKAEDLLGYGKEELLRMKVTDIHPREELDRVV
                     LHFSGAVAGRIDSLHDTRLLRKDGTVVPADITGCFVEYEGKRLAQGIFRNIAERKEME
                     ETLRKSEEQLRVLSENLADGMVYQINSGADGRQRVFTYVSPAVERFHGVKVEDAMMDP
                     SLIYDQVGAEYRTALKESETRACATMSRFEIDVPMRLPSGDVRWRRLISFPHLHSDGS
                     VMWDGIELDVTERRKMEEELAAHRDHLGKLVEERTARISEEVARRKEKEEQYLSLVES
                     IKEWVWEMNADLVHTYVSPRIREILGYEPEEFIGRAPFDFMAPGEPERIAPLAKKILS
                     GKEQFTSLGTAALHKNGQVVYLEASGRPFFDDKGTFLGYRGSCHDITDQKKTMDALRE
                     RERELTAKSEALEEVNAALKVLLRQREEDREELEARLVSNIREMVLPYIHKMRKDKLD
                     LRHRTFLDIVAANLNEIMSPFPTTIKQLNFTPREIEVASLIKEGRTTKEIAEFLGIAT
                     SAVDSHRNNIRTKLGLNNKKINLRSHLLSLG"
     gene            3325..4347
                     /locus_tag="PKJ17_02675"
     CDS             3325..4347
                     /locus_tag="PKJ17_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOD74903.1"
                     /translation="MLFFSAAVGALAFIMATASICLHARAQGSTGEVPAAGAAAQRIA
                     LAYVSTTHAVLAGVALEEGYYRQEGLEVTARRHSFGRSALEDMLAGNADLATAADIPI
                     MMAIMKGERISIIATIQTSTRDNAIVARKDAGIVTAADLTGKKIARAPGTTSDFFMDA
                     FLCAHGIGDKNVKKIDMKPEMFLDALVSGRVDAVSAFQPFLVRVEKELGDRVLSFYDK
                     NIYTWSFNIVAREEYVRRNPEKVRKLLRALVRAERFVKENPGRAQEIVTRFSGLEAGM
                     VREIWPSINFQVVLDQFLVLALEDESRWAMSSGRITGREVPNYLDFIHFDGLGSVKPD
                     GVRILR"
     gene            4369..>4960
                     /locus_tag="PKJ17_02680"
     CDS             4369..>4960
                     /locus_tag="PKJ17_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74904.1"
                     /translation="MQVRKRLYINSVVLVAAAVIVALLLAVGLYRVNDAFRALDIADD
                     ILNNLFLRSTFQSDYLRTGSERAKEQLLAQHARTMALLGRASRRFRDPADRKLIYDLT
                     GDHRSTAELFADIVENREKARKGTPSADLSGEIENRLLTQLNIRSYSAALNVQSLRAS
                     ARRILAAELRRTGVSIAVLIIIVAAVAGLSSWVTGRT"
BASE COUNT          923 a         1496 c         1455 g         1086 t
ORIGIN      
        1 ctccttctgg tattcgttga tgtcgacgac gctctcccag taatcggcga cgtcctcgag
       61 gccgtaggac cggcatatgt aggcgaggtt gagaatgtcc ttcttgaagc aggaaccgcc
      121 gaaccccacg ctcgcgttga ggaacttggg tcccaggcgg ctgtcgagtc cgatcgcccg
      181 cgatacctcg gccacatcgg cctccgtctt ctcgcacagc gccgagacgg cgttgatgga
      241 tgatatcctc tgggcgagga aggcgttggc gacgagcttc gagagttcgc tgctccagat
      301 gttgctctcg atgatcctct cccgcggcac ccagttgaga tagatatcga caacggcctt
      361 gcgcgcctct attccgcttg gtgtctcacg ggagcctatg aggacgcggt cggggaactc
      421 gaggtcccgc atcgccgtcc cttccgccag gaactcaggg ttggagagga catcgaaact
      481 gatatcgcca tccttggatg tcaggatacg ctccatcgcg agggccgtct tgacgggaat
      541 cgtacatttc tcgacgacga tcttcggcga ctcggaggcc tccctgatct gccgcgccgt
      601 cttctcccag tactggaggt ccgcagcctt tcccgcgccg gcgccgaagg tcttggtagg
      661 agtgttcacc cccacgaaga tgatctccga ttccctgatg cctttatcga tgtcggtctc
      721 gaagaagagg ttcttccccc gcgtcgcccg tatgatctca tcaagcccgg gctcataaac
      781 cggcaggtca tcggagttcc agcgctcgat cttttcatgg ttgatatcga ccacggtgac
      841 cctgtactgc gggcatttgt aggcgatcat ggccatcgtc ggcccgccga cataaccggc
      901 accgatgcac agaatattct tacgctccaa ggagaccctc catcgatagt tttgggtatt
      961 ctaccacaaa cacggtgttg cggggaagaa actccgtgat atctttcccc ctttcgtccg
     1021 cttatccgag ggagaggagg tgcgagcgga ggttgatctt cttgttgttc aggccgagct
     1081 tggtccgtat gttgttcctg tgggaatcga cggcgcttgt cgctatcccg aggaattccg
     1141 caatctcctt ggtcgtcctg ccttccttga tgagcgaggc cacctcgatc tccctggggg
     1201 tgaagttcaa ctgctttatc gtcgtcggga agggtgacat tatctcgttc aggttcgccg
     1261 cgacgatatc gaggaacgtc ctgtgcctca agtccagctt gtccttccgc atcttgtgta
     1321 tgtagggcag gaccatttcc cggatgttgg ataccagtct cgcctccagt tcctcccggt
     1381 cctcctcgcg ctgcctcaac aggaccttga gggcggcatt cacctcctcg agggcctccg
     1441 atttggccgt cagttcccgt tcgcgttccc tgagggcatc catcgtcttc ttctggtcgg
     1501 tgatgtcatg gcaactcccc cggtatccga ggaaggtgcc tttgtcatcg aagaaagggc
     1561 gcccactcgc ctcaaggtag accacctgcc cgttcttgtg aagggccgcg gttcccaggg
     1621 acgtgaactg ttctttgcct gacaggatct ttttcgccag gggagcgatc ctttccggtt
     1681 cgccgggggc catgaaatcg aagggggctc tgcctatgaa ctcctctggc tcatagccga
     1741 ggatttctcg tatccggggg cttacgtacg tgtggacgag gtccgcgttc atttcccaca
     1801 cccattcctt tatcgattcc acgagggaga ggtattgctc ctctttctct ttcctcctgg
     1861 caacctcctc actgatccgg gcggtgcgtt cttccaccag tttgccgaga tggtcgcggt
     1921 gcgccgccaa ctcctcttcc atcttcctgc gctcggtgac gtcgagttca atgccgtccc
     1981 acatgacgct cccatcggaa tgaaggtggg gaaaggagat aagacgccgc cagcggacat
     2041 cgcccgaagg aaggcgcatc ggcacatcga tctcaaaccg tgacatcgtc gcgcaggcgc
     2101 gggtttcgga ttccttgagc gccgtccggt actcagcgcc cacctggtcg tagatgagtg
     2161 aagggtccat catggcatcc tccaccttca caccgtgaaa cctctccacc gccgggctca
     2221 cgtatgtgaa cacccgctgc cttccgtcgg caccgctgtt gatctggtag accatcccgt
     2281 cggcgaggtt ctcgctgagg acgcgcaatt gctcctcgct tttccgcagg gtttcctcca
     2341 tctccttgcg ctcggcgatg tttcggaaga tcccctgggc caaccgcttt ccttcatatt
     2401 ccacgaagca tcccgtgatg tccgcgggaa ccaccgtgcc gtctttccgc agtagcctgg
     2461 tgtcgtgaag agagtcgatc cggcccgcca ccgctcccga gaagtgaagg accaccctgt
     2521 cgagttcttc ccgcgggtga atatccgtca ctttcatgcg cagcagttct tccttcccgt
     2581 agcccagaag gtcctccgcc tttctgttga cctcaaggaa gttcccttcg gggtcggcaa
     2641 cgattatcgc gtcgctcgcg tgctcgaaga gggtgcgata tctttcctca agccccgagg
     2701 gcacctcagg gcgggacctc ccacccgcat ccgatccctc caggtcggca acccgtctgc
     2761 gaagggagag gagttcctcg atgagttggt ctttcgtctt ctttccgtct ttcatatgat
     2821 cgattttcgt gcggccctcg acactacgta ctatcactat acaatgatgc gggatcattt
     2881 gcacgctccg atttcagagc agggcccgct cgctccgtgt cccgggcgct ccatcgcgga
     2941 tacgcgggcc ggcccggtcg attcgccgcg tgatgtccgg ggccctggaa ttgacacgtc
     3001 agggggtaat ctgatagcat cgagtaatga tccggaacgt tcccgtcacg tggtcttcaa
     3061 aggatacccg gaaaggccgg aggcggtgcg gccacggaag gcaccgtgat tcgcaaaacc
     3121 gccgcacctt atggctccct acgggtatct ttaccgttgc gcggcccgca agacccatct
     3181 cccttctccg tcgatgctcc gcgagctcgc tctgccggcc ctcttgttta caacaggagt
     3241 agaaaccccg gggacgcaag tcggcaacga catgtggcgc aaccctggtt cggccccggg
     3301 taggcaaagt gttgaatgag gaaagtgctc tttttttccg ccgcggttgg agcccttgcc
     3361 tttatcatgg caacggcgtc gatatgcctt catgcgcgcg ctcagggctc gacgggtgag
     3421 gtcccggcgg caggcgccgc tgcccaaaga atagctctcg cctatgtttc cacaacccac
     3481 gcggtcctcg ccggagtcgc gcttgaagag ggctattacc ggcaggaggg gttggaggtc
     3541 acggcgcgcc ggcattcatt cggaaggtcc gccctggaag acatgctggc ggggaacgcc
     3601 gatcttgcga ccgccgccga tatacccatc atgatggcga tcatgaaagg cgagaggata
     3661 tcgataatcg cgaccatcca aacctccacc agggacaatg cgatcgttgc gagaaaggac
     3721 gcgggcattg tcaccgcggc ggacctgacg ggaaagaaga tagccagggc tccggggacc
     3781 acctcggatt tcttcatgga cgccttcctt tgtgcccacg ggatcggcga caagaacgtg
     3841 aagaagatag acatgaagcc ggagatgttc ctcgatgccc tggtgagcgg ccgcgtcgac
     3901 gccgtttcgg cgtttcagcc ctttcttgtc cgggtcgaga aggaactggg cgaccgggtg
     3961 ctctcgttct atgacaagaa catctatacc tggtccttca atatcgtcgc cagggaggaa
     4021 tatgttcgca ggaaccccga aaaggtgagg aaactgctcc gggccctggt gagagcggaa
     4081 agattcgtca aggagaaccc cggccgggcg caggagatcg tgacgcgttt cagcggcctg
     4141 gaggcaggaa tggtccggga gatatggccc tcgataaact tccaggtcgt tctcgatcaa
     4201 ttcctggtgc tcgcgctgga ggacgaatcg cgatgggcga tgagcagcgg gaggataacc
     4261 gggcgcgagg tccccaatta cctcgatttc atccacttcg acggtctggg atcggtcaag
     4321 ccggacggcg tacggatact cagatagaaa ggaagcggag gccgggccat gcaggtcaga
     4381 aagaggttgt acataaactc cgtcgttctg gtggcagcgg ccgtcattgt cgccctcctt
     4441 ctggccgtcg ggctctaccg cgtcaacgat gccttccggg cattggacat cgccgacgac
     4501 atcctcaaca acctgtttct aagaagcaca ttccaaagcg attacctcag gacggggagc
     4561 gaaagggcga aagaacagct gctggcccag cacgcgcgga ccatggcgct tctcggtagg
     4621 gcttcgcgga ggttcaggga tcccgccgac agaaagctta tttatgatct gacgggggat
     4681 cacaggtcga cggcggaact tttcgccgac attgtggaga acagggagaa ggcgcggaag
     4741 ggaacgccct cggccgacct ttccggggag atcgagaaca ggctgctcac ccaactcaat
     4801 atcaggtcgt acagcgcggc cttgaacgtt cagtccctgc gcgcgtcggc gaggaggatc
     4861 cttgcggccg agctgagacg gacaggggtg agtatcgccg ttctcatcat catcgtggcc
     4921 gcggtggcgg ggctcagctc atgggtcacg ggccgtactc
//
LOCUS       DAOPOA010000079         4941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_18602_length_4941_cov_3.482194, whole genome shotgun sequence.
ACCESSION   DAOPOA010000079 DAOPOA010000000
VERSION     DAOPOA010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18602_length_4941_cov_3.482194"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2238)
                     /locus_tag="PKJ17_02685"
     CDS             complement(<1..2238)
                     /locus_tag="PKJ17_02685"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOD74905.1"
                     /translation="MELWVGALNLGFLYAFVAIGIYITFRIHDFPDITVDGTFTTGAA
                     VTSVLVVAGTHPLLALLASFLACAAAGSVTALINTRLNVNSLLAGILVMTGLYSINLH
                     IMGRSNIPLLHTPEFFALLNRTNPGMPQEIWTALWLTVIMALSWLAVSSFFKTDFGLS
                     MRASGNNPVMARAAGINVDRVKIFGVAIANGLVGISGSLVAQYQGFADIGMGIGTVVT
                     GLASVIIGESILKKHSVYAWIVSAIIGSVIFRFMIAGALYAGMDPIDLKLLTAIFVLI
                     TLVVTAKIGKRTGRVKKKHLVTAAAAAAVILAVLAGIRYGNLASVMSQQRVKIGVFQV
                     SENPSLTITRDSFLEEMKRLGYEDGKNATFLLENANGDLATANAIVDKFLHEKVDIFV
                     PISTSCTQVAVKKVKEHPVVFATVADPFVTGAGKTDTDHLPNVTGVYGPVPMDRTMEI
                     VTKILPGRIRIGVIWDPSQANSEVNVNNLKKALEAYPRVTFVGGNIAGSSEVYQAASS
                     LVRRGIDAFVLPTDNIVYSAFDSVVKAARRKKVPIIFNDVGHPEGGALVVFGYAYETS
                     GRQAAHLVNRIIKGEEPATIPFERYKKTELGVNLKVAKESGLRIPEEILSAATIIVDE
                     RGGIHRRDGASSVEKMPGRGRKRLALFQFTDNVILDVCAKGVVDGLKKSGALEKEGIT
                     VDHKMAHGEFPTAQSIAQEMVSKNYDYIVTVSTISLQVTANHNRKIPHVFAAVTDPIK
                     AGVAKS"
     gene            complement(2242..3456)
                     /locus_tag="PKJ17_02690"
     CDS             complement(2242..3456)
                     /locus_tag="PKJ17_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD74906.1"
                     /translation="MNDPLSPEQAVDKLLLAGRIDSITCREIEALFRNAIMAGKRLIV
                     ADMTAVSYVSSAGLRVFLSVQKELKKAGGEVCFLACPSTVHSVFEISGFTKIFRFLSS
                     ENEITRLLTSSAGEVEIKEIPYDRAALRVLTRDVPTGSLTLIGSEEKLPSASYGEGDV
                     VSVRAADMRFAAGFAALGHSFEDYKGYFGETIVLDGNIFVYPALRRSAVDFIVHTEGE
                     MGTVYHFLHGLSFTGDFAHIVSFEPGDNLFTLDALVESAHTVSSSPVIGITAIFESKG
                     LFGMRLKKIPLEENRRPGEEDIFSGKSFPEWVDYSIEAEDIYNLVVIAGISARQRDRV
                     TGDADRAIPREGRAHVHAVVFDKKPFNRMPDNFPNELRRIVTGMEPQRVVHLMGRSNI
                     GPGLMGIVELGG"
     gene            complement(3684..3863)
                     /locus_tag="PKJ17_02695"
     CDS             complement(3684..3863)
                     /locus_tag="PKJ17_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74907.1"
                     /translation="MGDKNSLVLYIIAFAVMVGGFVMIFLTRSTTVLAAGAVLIFIGL
                     FVFLFGREKAKKAKA"
     gene            4142..4339
                     /locus_tag="PKJ17_02700"
     CDS             4142..4339
                     /locus_tag="PKJ17_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74908.1"
                     /translation="MDLKSARDYLTKGAGFPPRRNVASGSGVPVAGCLFRDMMKDRGL
                     DGCRNRTCDCPFAGKKRREPH"
     gene            4626..4928
                     /locus_tag="PKJ17_02705"
     CDS             4626..4928
                     /locus_tag="PKJ17_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74909.1"
                     /translation="MDPNAPTVSRRTLRFFDGTQVAVNNLHEIMSELHSVGKEPNRET
                     VDEIIAGLEAMGNYISPSEATRREYRNVLLEEYREFVETHDKAKNDAAPSSGGPER"
BASE COUNT         1066 a         1356 c         1482 g         1037 t
ORIGIN      
        1 gctctttgcg accccggcct ttatggggtc tgtgacagcc gcgaagacat ggggtatctt
       61 cctgttgtgg tttgccgtca cctggagcga tatcgtcgac accgtcacga tgtaatcgta
      121 gttcttgctc accatctcct gcgctatgga ctgtgccgtg ggaaattcac cgtgggccat
      181 cttgtggtcg acggtgatcc cttccttctc cagggccccg ctttttttca aaccgtccac
      241 gacgcctttc gcgcagacgt cgagaatgac attgtccgtg aactggaaaa gggcgaggcg
      301 cttcctgcct cgtcccggca tcttttccac ggaagaggcg ccgtcccttc gatgtatgcc
      361 gcctctctca tcgacgatga tggtggccgc gctcaggatc tcttcgggta tgcggagccc
      421 cgactccttc gctaccttga ggtttacgcc tagctctgtc ttcttgtacc gctcgaaggg
      481 gatcgtggcg ggttcttctc ccttgatgat acgattcacg agatgcgcgg cctgcctgcc
      541 gcttgtttcg taggcatatc cgaagaccac cagggcaccg ccctcgggat ggccgacatc
      601 attgaagatg atggggacct tcttgcgccg ggcagccttg accacggaat cgaaggccga
      661 atagacgatg ttgtccgtcg gcaggacgaa ggcgtcgatg cctcgccgga ccagcgacga
      721 ggctgcctga tacacctccg aagagcctgc gatgttgccg cccacgaagg tgacgcgggg
      781 ataggcttca agcgctttct tgagattgtt gacgttgacc tcggaatttg cctgcgaggg
      841 gtcccagatg accccgatcc tgatgcgccc cggcagtatc ttcgtaacga tctccatcgt
      901 cctgtccatg ggcaccgggc catagacccc cgtcacattg gggagatggt cggtatcggt
      961 ctttcccgct ccggtgacaa aggggtctgc cacggtggcg aagaccacgg ggtgttcttt
     1021 gaccttcttg accgcgacct gcgtgcatga agtggagatg ggaacaaata tgtccacctt
     1081 ctcatgaagg aatttgtcga cgatggcgtt cgccgtcgcg aggtccccgt tcgcgttctc
     1141 cagcaggaag gtcgcgttct ttccatcttc ataacccagc cgtttcattt cctccaggaa
     1201 agagtcccgg gtaatagtga ggctcgggtt ttcactgacc tggaagaccc ctattttgac
     1261 cctctgctgc gacatgacgg aggcgaggtt cccataccgt attcccgcga ggacggcgag
     1321 gataacggct gccgccgcag ccgcggtgac gagatgtttc ttcttcaccc ttcccgttct
     1381 tttgccgatc ttcgcggtta caacaagggt tatgagaacg aagatcgccg tcagaagctt
     1441 gaggtctatg ggatccatac ccgcgtagag cgcgccggcg atcatgaacc ggaagatcac
     1501 ggagccgatg atggcgctta ctatccaggc gtagacggag tgcttcttga ggatggattc
     1561 gccgatgatg acggaggcaa ggccggtgac aaccgtcccg atccccatcc cgatatcggc
     1621 gaaaccctga tactgggcga ccaggcttcc cgagatgccg acgagcccgt tggcgatggc
     1681 aacgccgaag atcttgacgc ggtccacgtt gatccccgcg gcgcgcgcca tgaccgggtt
     1741 gttgccgctc gccctcatgg aaaggccgaa atcggtcttg aaaaaactgg agacggcgag
     1801 ccaggataag gccatgatga cggtgagcca cagcgccgtc catatctcct ggggcatgcc
     1861 gggattcgtc ctgttgagca gcgcgaagaa ctccggcgtg tgaagcaatg gtatgttgga
     1921 gcggcccatg atatgcaggt tgatggaata aagccccgtc atgacgagga tgcccgcaag
     1981 caggctgttg acattgagac gcgtgttgat gagcgccgtg acgctccccg ccgcggcgca
     2041 ggcaaggaaa gatgccagaa gggccagaag aggatgggtg cctgccacta cgagaacgga
     2101 ggtaacggcc gcccctgtcg tgaacgtccc gtcaacggtg atgtcgggaa agtcatgaat
     2161 gcggaaggta atgtaaatgc cgatggcgac gaaggcgtag agaaagccga ggttgagggc
     2221 gcctacccag agctccatcg actaaccgcc gagctccacg atacccatga ggccgggacc
     2281 gatgttcgac ctgcccatga ggtggacgac cctctgcggt tccatgccgg tgacgatcct
     2341 cctgagttcg ttgggaaagt tgtccggcat cctgttgaag ggtttcttgt cgaaaacgac
     2401 ggcgtgcacg tgggcgcggc cctcccgcgg gatggccctg tcggcatctc cggtcacgcg
     2461 gtccctctga cgggccgaga tgcccgcgat gacgacgagg ttatagatat cctcggcctc
     2521 gatggaatag tcgacccact cgggaaagct ctttcccgaa aagatatctt cttcgccggg
     2581 gcggcggttc tcctcgaggg ggattttctt gaggcgcatg ccgaagaggc ccttgctctc
     2641 gaagatcgcc gtgatgccta tcacgggtga ggacgatacg gtgtgagcgc tttctacaag
     2701 agcgtcgagc gtgaagaggt tatcgccggg ttcgaaggag acgatgtggg cgaagtctcc
     2761 cgtgaaggac aggccgtgga gaaagtgata gaccgtgccc atctctccct cggtgtgcac
     2821 gatgaaatca acggcggacc ttctcaaagc ggggtagaca aagatattgc cgtcgaggac
     2881 gatggtctct ccgaaatagc ccttgtagtc ctcgaaactg tggcccagag ccgcgaatcc
     2941 ggccgcaaac cgcatgtctg ccgccctgac ggacacaaca tccccttcac cgtaggaggc
     3001 tgacggaagc ttttcttcgc tgcctatgag tgtgagtgac ccggtgggga catcccgggt
     3061 gaggacacgc agcgccgcgc ggtcgtacgg gatctccttt atctcgacct ctcctgcaga
     3121 ggatgtgagg agtcgtgtta tctcgttttc ggaggaaagg aaacgaaaga tcttcgtgaa
     3181 tccgcttatc tcaaatactg aatgaacggt ggacgggcat gccaggaagc agacctctcc
     3241 ccccgccttt ttcagttcct tctgaacgga caggaaaaca cgcagaccag cgctgctcac
     3301 atagctgacc gccgtcatgt cggcgacgat gagcctctta ccggccatga tggcgttcct
     3361 gaagagggcc tcgatctccc ggcaggttat gctgtcgatc ctgcctgcga gcagaagttt
     3421 gtcgacggcc tgttctgggg acaaaggatc gttcatctgg agggtctcac cagtaagcta
     3481 tcattggaaa tcccactttg caaatctttt gtgtctaacc gggcggaacg gggaaggagc
     3541 cggcatgcac ggtcctccga gaggagcaaa actcaattca aaggggacgt atcggatccg
     3601 ttaaaggcga caacgatcgg cggtatgaag gacgcaaacc gggatttcaa gagtgaggtg
     3661 ttacagggtc taacacgagc cggtcaggct ttggcctttt tggccttttc ccttccaaag
     3721 aggaaaacaa agagcccgat aaagatgagc acggcgccgg cggcgagcac tgttgtgctc
     3781 cgtgtcagga agatcatgac gaaaccaccg accatgaccg cgaaagcaat gatatacagg
     3841 accaaggaat tcttgtcgcc catgccaacc cccgtcgctt aggatatttt actatagcat
     3901 acattccgtt tttatcaaaa aaaatactgc atcatttcgg gccattcagg tcttttcggc
     3961 aaaaaagtat taagaggtca tcgctggcgc cgataatacg gttgtattgg cacgttggga
     4021 tggaagggtc tgttttgagt accagccgga aatgccgccc agggtgagcc cggatggcac
     4081 tatgacccgg tgcgcgaaca gcgtatgacg agagcaacag gcgaaaggca ggaaacggac
     4141 aatggatctt aagagtgcca gagactatct aacaaaggga gccggctttc cgccgcggcg
     4201 caacgtggcg tccgggtcag gtgttcccgt ggcagggtgc ctcttccgcg acatgatgaa
     4261 ggaccgcggg ctggatggtt gccggaacag gacctgcgat tgcccctttg ccgggaagaa
     4321 acgccgggag ccgcactgac ttccgacggg agccggaaag aaaaaaaaca gcttgacaat
     4381 catttttcgg tggtcctgat atatatgtgg agtaccgcat agtatggtgc gaaaaatggt
     4441 tgaggacgac atatccgtaa cttcctccgc gatggcagtg tgcctggcgg catgctcctc
     4501 catatccgca ctcgaggtcc gggtcacacc gcatcacgca ctgaagaccg gtccgtcaga
     4561 ccggccggga ataccaggga gagcgctgca tgtctatttt tcaggttata gggtgagtta
     4621 cctgagtgga tccgaacgcg ccgacagtta gccggcgtac actcaggttc tttgacggta
     4681 cacaggtcgc ggtcaataat ctccacgaga taatgtcgga gctgcactct gtcggcaaag
     4741 agccgaacag ggagaccgtg gatgagatca ttgccggact ggaggccatg ggcaattaca
     4801 tttctccctc cgaggccacc cgaagggaat acaggaacgt cctcctcgag gaatacagag
     4861 aatttgtcga gacacacgac aaggcaaaga acgacgccgc tccttcttcg ggggggcctg
     4921 aacggtgaag gacgtctctc g
//
LOCUS       DAOPOA010000080         4881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_18885_length_4881_cov_3.892043, whole genome shotgun sequence.
ACCESSION   DAOPOA010000080 DAOPOA010000000
VERSION     DAOPOA010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18885_length_4881_cov_3.892043"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1886
                     /locus_tag="PKJ17_02710"
     CDS             129..1886
                     /locus_tag="PKJ17_02710"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOD74910.1"
                     /translation="MDGMKGEVIKVSGPLVVARGLSGARMFEMVRVGELGLFGEIIEI
                     KGEEYSIQTYEETEGIGPGQPVVRTGEPLSVELGPGLIRSIYDGIQRPLDVLAQDFGE
                     YIVRGTQRPALDRSKKWDFVPLARVGDAVKGGDILGTVQESALVSHKIMVPPGKSGTV
                     GKIGPVTGNVDTEVAVIQSPGGPFGVTMVQRWPVRDPRPTKRKVPPTETMTTGQRIID
                     MFFPITKGGTACVPGPFGSGKTVVQHQLAKWADAQIVVYVGCGERGNEMTDVLMEFPH
                     LKDPETGEPLMERTVLIANTSNMPVAAREASVFTGIAIAEYFRDMGYSVALMADSTSR
                     WAEAMREISGRLEEMPGEEGYPAYLGSRIAGFYERAGSVICIGSDDRRGAVSIIGAVS
                     PPGGDLSEPVVQTTLRVVKVYWGLDDKLAFARHFPAINWLSSYSLYQDIVDADADEKI
                     DRMWSVNRKRAMTILQKEAELDELVRLVGIDALSLEDRLLMQAARMIREDFLHQNAFD
                     DRDTYTSLRKQFLLLNLILRYYDEIRTGLADGATLEALLKLNVLEDITRARLIPEEEL
                     ERFGSIEGDIVNSIRALIW"
     gene            1911..3296
                     /locus_tag="PKJ17_02715"
     CDS             1911..3296
                     /locus_tag="PKJ17_02715"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOD74911.1"
                     /translation="MGNIIREYRTVKEIAGPLMLVDDVEGVTYGELADIKLEDGSLRR
                     GRVLEVHGRKAMVQVFEGTRGLSPQDVKVKFLAKGMELGMSIDMLGRVFDGSGRPIDD
                     GPAIIPEKYLNVNGNQINPYARDYPNEFIQTGISTIDLLNTLVRGQKLPLFSGSGLPH
                     SRIAAQIARQATVLKTGEKFAVVFAAMGITFEEAEFFIADFRKTGAIERSVLFINLAD
                     DPPIERLTLPRMALTTAEFLAFEKDMHVLVILTDITNYCEALREISAARKEVPGRRGY
                     PGYLYTDLSTIYERAGRIKGKNGSITQIPVLTMPEDDKTHPIPDLTGYITEGQIIILR
                     PLHTQGIYPPVDVLPSLSRLKDKGIGAGKTREDHSDVMNQLYAAYAQGKNARELAVVL
                     GESALTEEDVLFVKFSDAFEDRFVRQGEYENRTIEESLRIGWELLGMLPPNLLKRVRD
                     EFIERYYPKTS"
     gene            3312..>3763
                     /locus_tag="PKJ17_02720"
     CDS             3312..>3763
                     /locus_tag="PKJ17_02720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOD74912.1"
                     /translation="MRLAVNPTRMELLKLRRRLVVARRGHKLLKDKLDGLIKEFMNIA
                     REYRGLRQIVDEELPYVMKLFVLAEITSSRSITENALESVMQDLELTVHPARLMSVRI
                     QKLEIAYGEATGRYSLVHTSAELDMAIAKLREFLPRLLSMAQAEDTVRR"
     assembly_gap    3764..3773
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4000..>4881)
                     /locus_tag="PKJ17_02725"
     CDS             complement(4000..>4881)
                     /locus_tag="PKJ17_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOD74913.1"
                     /translation="GLPIREIGGMVMAILTISRQFGGGARAIALQVARDLGYEYVDRS
                     TMFKDISSEGPRWGTYAKDFDEHYPNVWERNDWSFKAFVALIQNLMYGYAQRDKVIIA
                     GTGATFLLKGVPHVLRVRIESSMEDRINRVQGDQVINESTARWIIDKVDADMSRSMYV
                     IYGKEWDDPKEFDSVFNSSTQTQDEIVEEIKKGLAAKEALRTKEALDILNLRAIASKV
                     KAAILTDPSFTVSYLDVSPKEEGMPRYGLIVRGFVHEMDDTQSIKEKVRSIAGDLPLE
                     FDVQYQMLPRFGHFDFR"
BASE COUNT         1024 a         1516 c         1447 g          884 t
ORIGIN      
        1 cccatgtacg aagacagtcc gaggacaggg tacaacgaga tgagaaaggc gatcgaacgc
       61 tccatgggca tagacctttt caagaaggaa gaagatgaga ccagcgaaca ggaatgaggg
      121 atgacaggat ggatggaatg aagggagagg tgataaaggt ctcgggtcca ctggtggttg
      181 ccagaggcct gtcgggcgcg cgcatgttcg agatggtccg cgtcggggaa ctggggctct
      241 tcggcgagat catcgagatc aagggagagg agtactcgat acagacatac gaggagacgg
      301 aaggcatagg tcccggtcaa cccgtcgtac gcaccggtga gccgctgagc gtcgagctgg
      361 ggcccggcct tatccgttcc atctatgacg gcatacagag acctctcgat gttctcgcgc
      421 aggatttcgg agaatacatc gtgaggggga cccaacgccc ggccctcgac aggtcaaaga
      481 aatgggactt cgtgccgctc gcccgcgttg gcgatgccgt gaaggggggt gacatcctgg
      541 ggaccgtcca ggaaagcgcc cttgtcagcc acaagatcat ggtcccgccg ggcaaatcgg
      601 ggaccgtggg aaagataggc cccgtaacgg ggaacgtgga tacggaggtc gccgtcatcc
      661 agagccccgg cggccccttc ggcgtgacga tggtccagag atggcctgtc cgcgacccgc
      721 gcccgacgaa gcggaaggtc ccgccgacgg agacgatgac gacggggcag cgcatcattg
      781 acatgttctt ccccataaca aagggcggca ccgcctgcgt cccggggccc ttcggaagcg
      841 gcaagaccgt cgtccagcac caactcgcca agtgggccga cgcgcagatc gtcgtgtatg
      901 tcggctgcgg cgagcgcggc aacgagatga cggacgttct catggagttc ccgcacctta
      961 aagacccgga aacgggagaa cccctgatgg agcgcaccgt cctcatcgcc aacacgtcga
     1021 acatgcccgt cgcggcccgc gaggccagcg ttttcacagg catcgccatc gccgagtact
     1081 tccgcgacat gggatactcc gtcgccctca tggccgattc caccagccga tgggccgagg
     1141 ccatgcggga gatatccggg cgtctcgagg agatgcccgg ggaagaaggc tatcccgcct
     1201 acctcggatc gcggatcgcc ggcttttacg aacgggcggg ctccgttatc tgcataggct
     1261 ccgacgaccg ccgtggcgcg gtctccatca tcggggccgt ctcccctccc ggaggcgacc
     1321 tttcggaacc cgtcgtccag acaacgctcc gtgtcgtcaa ggtttattgg ggactcgacg
     1381 acaaactggc cttcgccagg cacttcccgg ccatcaactg gctgtcgtcc tactcgctct
     1441 accaggacat cgtcgacgcg gacgcggacg agaagataga caggatgtgg tccgtcaaca
     1501 ggaaacgggc catgaccatc cttcagaagg aagcggaact ggacgagctc gtcaggctcg
     1561 tcggcatcga cgccctgtcc ctggaggaca ggctcctgat gcaggctgcc aggatgatcc
     1621 gggaggattt tctccaccag aacgccttcg atgaccgcga cacctacacg tcgctcagga
     1681 agcagttcct gcttctcaat cttatcctgc gctactacga tgagatacgc acggggcttg
     1741 ccgacggagc cacgctggag gcccttctga agctcaacgt tctcgaggac ataacacggg
     1801 cgaggctcat tcccgaggag gaactggaaa ggttcggctc catcgaaggg gacatagtga
     1861 acagcattcg cgcgctgatc tggtaacgag attacgaggt agatgagacg atgggaaaca
     1921 tcattcggga gtacaggaca gtaaaggaga tcgccgggcc cctcatgctc gtcgacgacg
     1981 tggaaggcgt tacctacggg gagcttgccg acataaaact ggaggacggc agcctccgcc
     2041 ggggccgcgt cctggaggtg catggcagaa aggccatggt gcaggtcttc gaggggacac
     2101 ggggactctc cccgcaggat gtgaaggtga agttcctcgc caaggggatg gagctcggca
     2161 tgtccatcga catgctcggc cgcgtcttcg acggctccgg ccggcccata gacgacggcc
     2221 ccgccatcat cccagagaag tatctcaacg tcaacggcaa ccagatcaat ccctacgccc
     2281 gggactaccc gaacgagttc atccagacgg gcatttcgac gatcgacctt ctcaacacac
     2341 tcgtgagggg ccagaaactg cccctcttct ccggctcggg cctgccccat tcccggatag
     2401 ccgcccagat cgcgagacag gcgacggtcc tcaagacggg cgagaagttc gccgtcgtct
     2461 tcgccgccat gggcatcaca ttcgaggaag cggagttctt catcgccgac ttcaggaaga
     2521 cgggggccat cgagcgttcc gtccttttca taaaccttgc cgacgacccg cccatagaga
     2581 ggctcaccct cccccgcatg gcgctgacaa cggcagagtt ccttgccttt gagaaggaca
     2641 tgcatgtcct cgtcatcctc acggacataa ccaactattg cgaggccctc cgggagatct
     2701 cggcggcaag gaaggaagtg cccggacgcc gcggataccc cggttatctc tataccgacc
     2761 tttcgacgat ctatgaacgc gcgggccgca tcaagggaaa gaacgggtcc ataacgcaga
     2821 tccccgttct caccatgccc gaggacgata agacgcaccc catccccgac ctgacgggct
     2881 acatcaccga ggggcagatc atcatcctcc gccctctcca cacccagggc atctatccgc
     2941 ccgtcgacgt ccttccgtcc ctctcgcgac tcaaagacaa gggcatcggg gccggaaaga
     3001 cgcgggagga ccactcggac gtcatgaacc agctctacgc ggcctatgcc cagggaaaga
     3061 acgcgaggga gctcgccgtc gtgctcggag aatcggcgct gacggaagag gatgtccttt
     3121 tcgtgaaatt ctccgatgcc ttcgaggacc gcttcgtccg ccagggcgaa tacgagaacc
     3181 ggaccataga ggaaagcctg cgcatcggct gggagcttct cggcatgctc ccgcccaacc
     3241 tcttgaagcg tgttcgcgat gagttcatcg aacgctacta cccgaagacc tcgtgatccc
     3301 ggaggattgc cgtgagactg gccgttaacc cgacacgaat ggagcttctg aaactcaggc
     3361 gccgcctcgt cgtcgcccgc cggggacaca aactcctcaa ggacaagctc gacggcctga
     3421 taaaggagtt catgaacata gcccgtgagt accgcggcct ccgccagatc gtcgacgaag
     3481 agctccccta cgtcatgaag ctcttcgttc tcgccgagat cacttcgtcc cgctccatca
     3541 ccgagaatgc cctcgaatcc gtcatgcagg acctcgagct cacggtccat ccagcgcggc
     3601 tgatgagcgt aaggatccag aagctggaga tcgcctacgg cgaggcgacg gggcgctact
     3661 ccctggtgca cacgtcggcc gagctggaca tggccatcgc gaagctccgc gagttccttc
     3721 ccaggctcct ttccatggct caggccgagg atacggtccg ccgnnnnnnn nnnaaaacgc
     3781 gacggcgcgt gaacgccctc gaacactctt tcattcctcg cctgaccgag accatccgct
     3841 tcatcacgtc caaactcgag gagatggagc gctcgacgac aagctctctc atgaaggtaa
     3901 aggcccagcg gctggcccgc gaggcggcac gggaggcgtg acaaaaaaac tccccggcgg
     3961 ttgtctccac cggggagcag ggattcgctt tgtccaagcc tacctgaaat cgaagtgacc
     4021 gaaccgtgga agcatctgat actgtacgtc gaactcgagc ggcaggtcac cggcgatgct
     4081 tctgaccttt tcctttatgc tctgtgtgtc gtccatctca tgcacgaagc ccctcacgat
     4141 gagcccgtag cggggcattc cctcctcttt gggagaaacg tcgagatagg aaacggtaaa
     4201 ggaggggtcc gtcaggatcg cggcttttac cttcgacgca atcgcccgga ggttcaggat
     4261 gtccagggct tccttcgttc tcagggcttc cttcgccgca aggccctttt tgatctcttc
     4321 cacgatctca tcctgcgtct gtgtcgagct gttgaacacg ctgtcgaact ctttcgggtc
     4381 atcccattcc tttccgtaga tgacgtacat ggaacgggac atgtcggcgt cgaccttgtc
     4441 gatgatccac cgggcggtgc tctcgttgat cacctggtct ccctgcaccc tgtttatgcg
     4501 gtcctccatg gagctctcta tccggaccct gaggacgtga gggacccctt tcagcagaaa
     4561 ggtggcgccc gtgccggcga tgatcacctt gtccctttgc gcgtagccgt acatgaggtt
     4621 ctggatgagg gcgacgaagg ccttgaacga ccagtcgttg cgctcccaca cgttcgggta
     4681 atgctcgtcg aagtccttcg cgtacgtacc ccatcgcgga ccctccgacg agatgtcctt
     4741 gaacatggtg gagcggtcga cgtattcgta ccccaggtcc cgggcgacct ggagcgcgat
     4801 cgccctggct ccgccgccga actgccgtga aattgtcaat attgccatga ccataccccc
     4861 tatttcgcga atgggaagac c
//
LOCUS       DAOPOA010000081         4834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_19167_length_4834_cov_3.002929, whole genome shotgun sequence.
ACCESSION   DAOPOA010000081 DAOPOA010000000
VERSION     DAOPOA010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19167_length_4834_cov_3.002929"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..759)
                     /locus_tag="PKJ17_02730"
     CDS             complement(208..759)
                     /locus_tag="PKJ17_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOD74914.1"
                     /translation="MLEVRFHGRGGQGAVTSAEIIAQAAIKQGMNAQAFPSFGPERRG
                     APVQAFLRVSDKPIRLRSKVYKPDSVLILDPTLITSANPTAGLKKGGYIIINSNKSVE
                     ELKNTFPGQNIIAVDASKIAREELGVPITNTTMLGALVRATRVVELSTLEEPVRNRFG
                     VNGQKNINAYTRAYNEATVIEAE"
     gene            complement(773..2218)
                     /gene="cysS"
                     /locus_tag="PKJ17_02735"
     CDS             complement(773..2218)
                     /gene="cysS"
                     /locus_tag="PKJ17_02735"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOD74915.1"
                     /translation="MALKVYNTISGRKEDFTPVTEGKVKLYVCGVTVYDHCHIGHARS
                     AIVFDTIYRYLCSKGFDATYVRNFTDIDDKIIKRSHAENIPWQEVGETYIRSFYEDMD
                     ALGVLRPNHEPRATEHIADMIDLVQALIDKGHAYNIDGDVYFSVDSFRNYGQLSGRPL
                     EEMISGARVEVDEKKKNPLDFALWKASKEGEPSWDAPFGAGRPGWHIECSVMSTLYLG
                     NPFDIHGGGKDLVFPHHENEKAQTEAATGKKFVNYWVHNGFVNIDREKMSKSIGNTLL
                     IRDFLREYHPEVLRLFYLSNHYRSPVDYSGQSIEDINTALHRLYYTRERALEAQKGKT
                     FEERSYPQADDIARKFTEAMDDDFNTALALSHVFELSKEINRMVDEKDESAVALIASA
                     EAVYESLAATLGLLGDDLASLESREKVRHLARMGLDVSFVEDAINDRVEARKNKDFQK
                     ADEIRNMLADKGIMLLDTPKGTQWRIKTSVS"
     gene            complement(2272..3669)
                     /locus_tag="PKJ17_02740"
     CDS             complement(2272..3669)
                     /locus_tag="PKJ17_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOD74916.1"
                     /translation="MEIDDIIVGRRYREELGDMEDLKRSISELGLLQPIVIRKGTNEL
                     VAGYRRLKALTELGITSLVEGTHVNLIDIESLVLGEHDENICRKDFTISEKVAIFEAV
                     KEEQLRIGMERQILAGTRGPGAGDGAAGDHSGVDGTDDANAAGKLVNRTHGHARSHAA
                     KVVGLHQNTMRKASEIVAAAKKEPRRYGHLVREMDLSGKVDPVFQKYLQEKARPAAGA
                     HGLCPSLSRLLADREGSVTDALCSAMACIPVDRQEELARRFPVALHLVRPADLDQAAK
                     KRSGGGGLGDLPEDEVFALLDDTLGRVEAASREWVFMPQVRQVASIVMDVAKDQGAPT
                     LDKLDFFMIGLAGFVDMTCNYLQVARKDGIIEELPVDVLTRYLAVIDAFEMLYESKLP
                     RQIGALRIKECGLRPGEMPDTGTLRDHANAMKANEMITDAAEGSIPYYHERIANLIQS
                     FREGARQLTLAGRNA"
     gene            complement(3982..4347)
                     /locus_tag="PKJ17_02745"
     CDS             complement(3982..4347)
                     /locus_tag="PKJ17_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74917.1"
                     /translation="MKVEATKMVAKGGPGTADILLSYVDRYMAGLGPYVSSVFFILLL
                     KSDADRRKRGLIRSRGLSINEIADAAGISRRKAIDALKVLKGRWMIVQRKGRGRGNKN
                     HYYFLPMERWSNPGSLEKR"
     gene            complement(4344..>4834)
                     /locus_tag="PKJ17_02750"
     CDS             complement(4344..>4834)
                     /locus_tag="PKJ17_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74918.1"
                     /translation="VIDGVVRRILGRVDRGHYVGDQLNDYIQDATVVLMQLARQEKLD
                     LTKSAGEIASYICLWIEQRVKRMARKDQRWRFSLLETTDDIEGSAGDIAVEEGTANSW
                     NPGWTEAYHPPGPATEDTSAAGLWEKVCVAEKRISMTASGARKSERNVLSIFGPSRSE
                     KV"
BASE COUNT          911 a         1436 c         1333 g         1154 t
ORIGIN      
        1 ggttatgatg ccgttggcga tatggactgt tccaggaaag acggtctcgt tatggagatc
       61 gacgatatta ccggagatgg tcttcattga aagacccgaa gtatcgaata accaataacc
      121 aaattccagt gaccagggga aagacctttg ccgccggctg tctcttgact ggtcattgga
      181 ggttggtcat tggttattgt aagtatatca ctccgcctct atcactgtcg cttcgttgta
      241 tgccctggtg taggcgttga tgttcttctg gccgttgacg ccgaagcggt tgcgaaccgg
      301 ctcttccagc gtgctgagct cgacgacgcg cgttgcccga acgagggccc cgagcatcgt
      361 tgtgttcgtt atgggaacac ccagctcttc cctggcgatc ttcgacgcgt cgacggcaat
      421 gatgttctgc ccgggaaatg tgtttttcag ctcttccaca gacttgttgg aattgatgat
      481 gatataacca cccttcttga gacctgccgt cgggtttgcc gaggtgatga gggtggggtc
      541 caggatgaga acgctgtcag gtttgtagac ctttgacctc agcctgatgg gcttgtcgga
      601 tacacgcaaa aaagcctgga caggggctcc gcggcgctcg ggaccgaagc tcgggaatgc
      661 ctgcgcgttc atgccctgct tgatggcagc ctgtgcgatg atctcggcgg acgtaacggc
      721 tccctgcccg cctcttccgt gaaatctgac ctctaacata tactctctcc ttttaagaaa
      781 ctgaagtctt tatccgccac tgtgttcctt tgggtgtgtc aaggagcatg atccccttgt
      841 ccgccagcat gttcctgatc tcgtcggcct tttgaaagtc tttgttcttc cgtgcctcaa
      901 cacggtcgtt gatggcgtct tcgacgaaag aaacgtcaag gcccatgcgg gccagatgcc
      961 gcaccttctc acgggattcc agggatgcca gatcgtcgcc gagaagcccc agggtagcgg
     1021 ctagcgattc atagacggcc tccgcggagg cgatgagggc aacggcgctc tcatccttct
     1081 cgtcgaccat cctgttaatc tctttagaaa gctcaaaaac gtgcgaaagg gcaagggcgg
     1141 tgttgaaatc gtcgtccatc gcctcggtga attttcgggc gatgtcgtcg gcctgcggat
     1201 aggaccgctc ttcgaatgtc ttgcccttct gcgcctcgag cgcccgttcc cgggtataat
     1261 agagcctgtg cagtgccgtg ttgatgtcct cgatggactg cccgctgtag tctaccgggc
     1321 tgcggtagtg attggacagg tagaaaagcc gaaggacctc gggatggtat tccctgagga
     1381 agtccctgat aagcagcgta ttgcctatgg acttcgacat cttctcacgg tcgatgttga
     1441 cgaacccgtt gtgcacccag tagttgacga acttctttcc cgtcgccgcc tcggtctggg
     1501 ccttctcgtt ctcgtgatgg gggaagacaa ggtccttccc gcctccatgg atatcgaagg
     1561 ggttcccgag gtaaagggtg ctcatgacgg agcattctat gtgccaaccc ggcctgccag
     1621 caccgaaggg ggcatcccag gagggttcgc cctccttgga tgccttccac agagcgaagt
     1681 cgagggggtt cttcttcttt tcgtccacct cgaccctggc cccggagatc atttcctcga
     1741 gagggcgacc ggagagctgg ccgtagttgc ggaaactgtc cacggagaag tagacatccc
     1801 cgtcgatatt gtaggcatgg cctttgtcta tcagggcttg cacgaggtct atcatgtccg
     1861 cgatatgctc cgtggcacga ggctcgtggt tcggccggag cacccccagg gcatccatgt
     1921 cctcatagaa agagcgaatg tacgtctcgc ccacctcctg ccagggtatg ttctcggcat
     1981 gagatctctt gattatcttg tcgtcgatgt cggtgaagtt cctgacgtag gtagcgtcga
     2041 agcccttcga gcagagatac cggtatatgg tgtcaaagac gatggcgctc ctcgcgtgac
     2101 cgatatggca gtgatcgtac acggtgaccc cgcagacata gagcttcacc ttcccttccg
     2161 tgacgggggt gaaatcctct ttcctgcctg atatcgtatt gtaaaccttc agggccatag
     2221 tgttgaccgt atttttacca cagctgtcgc ggccttgtca aagcaattga atcaagcgtt
     2281 ccgccccgcg agagtgagct ggcgggcgcc ctcacggaag ctctggatga ggttcgcgat
     2341 ccgctcatgg tagtagggga tgctgccttc cgcggcgtcc gttatcattt cgttcgcctt
     2401 catcgcgttg gcatggtccc tcaaggtccc cgtatcgggc atctcgccgg ggcgaagccc
     2461 acactccttg atccgcaacg cgccgatctg ccgggggagc ttgctctcgt agagcatctc
     2521 gaaggcatcg atcaccgcaa ggtatctcgt gagaacgtca acggggagtt cctcgatgat
     2581 cccgtccttc ctggccacct ggagataatt gcacgtcata tcgacgaacc cggcaagacc
     2641 gatcataaag aaatccagtt tgtcgagggt cggggcccct tggtcctttg ccacgtccat
     2701 gacgatggaa gctacctgtc tcacctgcgg catgaagacc cattcgcgcg aagctgcctc
     2761 gacccgtccc agagtgtcat cgaggagcgc gaagacctca tcttccggga ggtctcccag
     2821 cccgccgcca ccggaccgct ttttagccgc ctgatcgaga tcggcgggcc tgacaaggtg
     2881 gagagcgacg ggaaaacggc gcgcaagctc ctcctgacgg tcaacgggga tgcatgccat
     2941 ggcgctgcac aaggcatcgg tgaccgaacc ctctcgatcc gcgagcaggc gggaaagaga
     3001 ggggcaaagc ccgtgcgctc cggccgcggg ccgcgctttt tcctgaagat atttctggaa
     3061 gactgggtcg accttccccg agaggtccat ctctctcaca agatgaccgt accgccgggg
     3121 ctcctttttc gcggcggcca cgatctccga ggcttttctc atcgtattct gatggagtcc
     3181 caccaccttc gccgcatggg acctcgcatg tccgtgtgtg cgattgacca gtttccccgc
     3241 tgcattcgcg tcatcggtcc catcgacccc ggagtggtcg ccggcagcac cgtcacctgc
     3301 acctggtccg cgggtaccgg ccaggatctg cctttccatt ccgatcctca attgctcttc
     3361 cttgaccgcc tcgaagatgg ccactttttc cgatatcgtg aagtccttcc tgcagatgtt
     3421 ctcgtcatgc tcgccaagta caaggctctc tatgtcgatg aggttgacgt gggtgccctc
     3481 gacgagggag gttatgccga gctccgtgag ggctttgagc ctccgatagc cggcaacaag
     3541 ctcgttcgtt ccctttcgga tcacaatcgg ctggaggagc cccagttccg atatggagcg
     3601 cttcaggtcc tccatgtctc cgagttcctc cctgtaacgc ctgccaacaa ttatatcatc
     3661 aatctccatc tgcccctccc tcaccggccg tttgttcccg attccgctgc gtatgatggt
     3721 catgtcgtgc actctatata atatatcctt ctcacgcgac gcaccgtcat ttcagggatc
     3781 tcttcgcgtg gcgggatgga acacccctgt acaattgggg caggggccac aagtggcccc
     3841 tatcccgtgg atcacggtgg tttcggggat tatcgcgcac cgccccaagg taaaggtgca
     3901 caggatgcat gttggagatg aatgccttcg ggtatccagg ccctttcccg cgggcatgga
     3961 cccggtagcg gtgctccagg ctcaccgttt ttcaagagat ccggggttgg accatctctc
     4021 catgggaaga aaatagtaat gattcttgtt cccccttcct cttcccttgc gttgcacgat
     4081 catccacctg cccttgagga ccttgagggc atcgatggcc ttcctccttg atattcccgc
     4141 ggcatcggcg atctcgttga tggacaggcc cctgcttctg atgagtcccc tcttccgtct
     4201 gtcggcgtcg gacttcagga gaagaatgaa gaaaacggag ctcacgtagg gcccgagccc
     4261 tgccatgtaa cggtccacat aggacaacag gatatccgcg gttccgggac caccctttgc
     4321 caccatcttc gtcgcctcga ccttcacacc ttctccgagc gcgagggacc gaatatggaa
     4381 aggacattgc gttcgctctt gcgcgcaccc gaggcggtca tcgaaatccg tttttcagcc
     4441 acgcagacct tctcccagag tccggcggcc gacgtgtctt ccgtggccgg tccgggcggg
     4501 tgatatgctt cagtccatcc cggattccaa ctgttagcgg tcccctcttc cacggcgatg
     4561 tcaccggcgg aaccctcgat atcatccgtg gtttccaaga gcgagaacct ccaacgttga
     4621 tcctttctgg ccattctctt gaccctctgc tctatccaca aacagatgta ggaagcgatc
     4681 tctccagcgg atttcgtgag gtcgagcttt tcctgtctgg ccaattgcat caggaccacc
     4741 gtcgcatcct gaatgtagtc attcagttga tcgccaacat aatgtcccct gtcgaccctg
     4801 ccgaggatcc tcctgacgac accatcaatg acct
//
LOCUS       DAOPOA010000082         4810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_19304_length_4810_cov_3.510200, whole genome shotgun sequence.
ACCESSION   DAOPOA010000082 DAOPOA010000000
VERSION     DAOPOA010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19304_length_4810_cov_3.510200"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PKJ17_02755"
     CDS             complement(<1..447)
                     /locus_tag="PKJ17_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOD74919.1"
                     /translation="MDYSIKIGGEAGQGIQTVGDALARVFSRLGCHVFTSQDYESRIR
                     GGHNFYQIRVSDRPVRAPRGPLDILVAFDRASITVHQGELVEQGIIVYDSSQLKESFS
                     GSQFLDVPFVKLATDAGAKPIAANTVATGAILGMLGFSIDPLYDIIT"
     gene            546..2687
                     /gene="rnr"
                     /locus_tag="PKJ17_02760"
     CDS             546..2687
                     /gene="rnr"
                     /locus_tag="PKJ17_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOD74920.1"
                     /translation="MRKKGTVKQGKRDAEPITDTQVLRVLSSARRPVGIDELGDVFGR
                     GRPGRRDIMRHLRSLIAKGRVTELKNKRFGLTREMDLLSGTLWCTRGGNAFVIPDSGD
                     EKDVFVSSRNLNHAVHGDRVTVRLDHYRGKLEGKVIRILERKSSIIIGFTKIADNMIY
                     VIPEDFRYQTKYLVSRPRREVRDGELVVARVTAFPGEKVEPECAITRVLGDLTSVAAI
                     GRFIEYKHSLSRRFPKHAEDEARSAVTSLTQKGRSDQRDLGFVTIDGKNARDFDDAVA
                     IERGRSGFTLHVAIADVSHFVKTGSLLDQEAGKRGMSVYFPDRVLPMLPKTLSNGICS
                     LNPLEDRYTVTATIEFDRNGVVLGASFRPSLIRSAMRLTYEAVEKAVVENDGKTRQKI
                     EPCLAALTDMAELAGLITCTREKRGSIDFDLPEPEVILDIKGGIRQIVRSRRLYSHRI
                     IEEFMIAANEAVARYLEAKNVPALFRVHEPPEREKLRDFERLLQGLGIGHRKTDTSQA
                     LQDVLSTVKDTNYEFIVNRVLLRSMKQARYFSRNKGHYGLASGSYLHFTSPIRRYPDL
                     VCHRILKAALDGRQPVYDEEQLAAMAGHLSERERLVMEAERELEDRIRILYMKDRVGD
                     VFEGVISHVTSFGFFVELSEVFVEGLVLISSLGDDYYRFEEERFRITGRRTRKTYRVG
                     DAVTIRVVMADVATNRLHFEVAAGGGADKKL"
     gene            2856..>4810
                     /locus_tag="PKJ17_02765"
     CDS             2856..>4810
                     /locus_tag="PKJ17_02765"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD74921.1"
                     /translation="MRRPGLVNVFDLEDIRDLQNTLAEAFGVASVILDQSGKAVTDHV
                     GFSSVLNTLTRKDGLTFLTCMEPSLVAGSIRKGEMVIEQCPRCGLLYGIIPITVGTHR
                     LASWLVGQVRDRDTDVEELVAGADAMGVDGEAYRRALADVPGMTREQFEKVCRAISLM
                     ARQLSLLAMQNLRQARDISAREEVEEALRMSELRYRQMFNNDPFPSLVYDIETLQVVD
                     VNDIALTSYGYSREEFLSLTVMDLVASESIPALLDILSRRPTSRCAASSRHVKKDGTI
                     IDVEVTGHLLDFPGKQYRMITVIDVTERKMTEEHLNFTQAVVDRVVDCIFWLDRYARI
                     IYVNEAACRTTGYSRDELLSMTVFDIDPTLTKEGWESHWKQKKACGSLLTESYHRSKD
                     GRVYPVEISGSYLAYGNREYNCTFVRDITERRKVDQQLLLTQFTVDKAAAIILWIGED
                     ARILYANDEACRSTGYTREDMLGLALPVIDPSFAPPVWNDHWNDVKERGTIIFESFQK
                     RKDGSLYPVEIKGNYMEYGGIGYICSIAFDITERKKTDELLRMTSFSMDQSAIPTFWL
                     DMDGNALRVNKAALISLGYTEEEMLGKNVRTWDEDFPHDLWDSHIFPKLKARHSLTME
                     SKCKRKNGSKFPVELNLNYMTYAGNEYV"
BASE COUNT         1097 a         1437 c         1380 g          896 t
ORIGIN      
        1 ggtgatgatg tcgtagagcg ggtcgatgga aaagcccagc attcccagga tggcccccgt
       61 ggccaccgtg ttggccgcga tcggcttcgc ccccgcatcg gttgcaagct tcacgaacgg
      121 aacatccaag aattgagacc cgctgaaact ctccttcaac tgggaggaat cgtatacgat
      181 gatcccctgt tccacgagct cgccctgatg gaccgtgatg gacgcccggt cgaaggccac
      241 caggatgtcg aggggcccgc gcggcgcgcg gacgggccgg tcgctcacgc ggatctgata
      301 gaagttgtgc cccccgcgga tgcgcgactc gtagtcctgg ctcgtgaaaa catggcatcc
      361 cagcctggag aacaccctcg ccagggcatc gcccaccgtc tggatcccct gtcccgcctc
      421 gccgcctatc ttgatcgaat agtccatacc gcccagaaca acattatagt gggttgcctg
      481 gcaaatatca accgctcctt cctgccgagg attggaaaca ttgaataaac gtgatataac
      541 ctatcatgcg gaaaaagggg actgttaaac agggaaagag ggatgccgag cccatcaccg
      601 atacccaggt cctccgggtg ttgtccagtg cgcgacgacc cgtaggcatc gatgagttgg
      661 gagatgtttt cgggcgtggc agacccggac gccgggatat catgcgtcac cttcgctctc
      721 ttatagcaaa gggccgcgtg acggagctga agaacaagag gttcggtctc accagggaga
      781 tggacctcct ttccggaacc ctgtggtgca cccgcggcgg caacgccttc gtcatacccg
      841 attcagggga tgagaaagac gtcttcgtgt cgtcccggaa cctcaatcac gccgttcatg
      901 gcgaccgcgt caccgtgaga ctcgaccact atcgggggaa gctggaagga aaggtcatca
      961 ggatactaga gagaaaatca tctattatca taggcttcac aaagatagca gacaatatga
     1021 tttacgttat tccggaagac ttccgatatc aaacaaaata tcttgtatca cgaccacgca
     1081 gggaagtccg ggacggggaa ctggtggtcg cccgggtaac agcctttccc ggcgaaaagg
     1141 tcgagcccga atgcgccatc accagggttc tcggcgacct cacgagcgtc gccgccatcg
     1201 ggaggttcat cgaatacaag cactcccttt cccgtcgctt cccgaaacat gccgaggacg
     1261 aggcgcgctc cgccgtgact tcccttactc agaaaggccg ctcggaccag agggaccttg
     1321 ggttcgtcac catcgacggg aagaacgcga gggatttcga tgacgccgtc gccatcgagc
     1381 gcgggagatc ggggttcact ctccatgtgg ccatcgccga tgtctcccac ttcgtgaaga
     1441 ccggctcatt gctggaccag gaggccggaa agcgagggat gagcgtctat tttcccgacc
     1501 gggtgctccc gatgcttccc aagactctct ccaatgggat ctgcagtctc aatcccctcg
     1561 aggacaggta taccgtcacg gccaccatcg agttcgacag gaacggcgtg gtgctcggcg
     1621 cctcctttcg accttccctg atacgcagcg ccatgcgcct cacctatgaa gccgtcgaga
     1681 aggccgtcgt cgagaatgac gggaagacgc ggcagaagat cgaaccctgc ctcgcggccc
     1741 tgaccgacat ggccgaactc gccgggctta tcacctgcac ccgggaaaag aggggaagca
     1801 tcgatttcga ccttcccgaa cccgaggtca tcctcgacat caagggcggc atccgccaga
     1861 tcgtccggtc ccggcgtctc tattcacacc gcatcatcga ggagttcatg atagcggcca
     1921 acgaggccgt ggcgcgctat ctcgaagcga agaacgtccc ggcgctcttc agggtccacg
     1981 aacccccgga aagggagaag ctccgcgact tcgaacgtct cctccagggg ctgggcatcg
     2041 ggcacaggaa aaccgacact tcccaggccc tgcaggacgt gctgagcacc gtgaaggaca
     2101 ccaattacga gttcatagtc aacagggtcc tgttgaggtc gatgaaacag gcccgttact
     2161 tctcccggaa caaggggcat tacggccttg cctccggcag ctacctccat ttcacatcgc
     2221 cgatacgcag gtaccccgac cttgtctgtc acaggatact gaaggctgcc ctggacggca
     2281 gacagcccgt ctacgacgaa gagcaacttg ccgcgatggc cggtcacctg tctgagcggg
     2341 agcgactggt catggaggcc gaacgggaac tcgaggacag gatccggata ctctacatga
     2401 aggacagggt cggtgatgtc ttcgaaggcg tcatctccca cgtcacttcc ttcgggttct
     2461 tcgtcgagct ttccgaggtg ttcgtggagg gtctcgtcct catctcgagc cttggcgacg
     2521 actattaccg tttcgaggag gagcgtttca ggatcacggg gagaaggacg aggaagacct
     2581 atcgcgtcgg tgacgccgtg acgatacggg tcgtcatggc ggatgtggcc accaacaggc
     2641 tccatttcga ggtggcagcc ggcgggggcg ccgacaaaaa gctttagctt atatcggatc
     2701 ctgatgataa tatagtacga caagaacatc cttgagagac gaccatgccc gcggaagaca
     2761 aacttagatc ctcaaagcac gcggggcgcc gcaagggacg acccgaaaaa aagccttccg
     2821 tcgcgtcggg caggaataca ccaaagacga cgaaaatgcg ccgcccaggc ctcgtcaatg
     2881 tcttcgatct cgaggatata cgggacctcc agaacactct cgcggaggcc ttcggcgtcg
     2941 cttcggttat cctggaccaa agcggcaagg cggtcaccga ccatgtcgga ttctccagcg
     3001 tcctcaacac cctcaccaga aaggacggcc tcaccttcct cacctgcatg gagccatcgc
     3061 tggtcgccgg cagcatacgg aagggagaga tggtcataga gcaatgcccg cgatgcgggc
     3121 tcctgtacgg tatcataccc atcaccgtgg gtactcaccg tcttgcaagc tggctcgtgg
     3181 gtcaggtgcg ggacagggat accgacgtgg aagagcttgt cgcgggggcg gacgcgatgg
     3241 gagtggatgg tgaggcgtat cgccgtgccc tcgcggatgt gcccggaatg acacgagagc
     3301 agttcgaaaa ggtatgccgc gccatttccc tcatggccag gcagctttcc ctcctcgcga
     3361 tgcagaacct ccgccaggcc cgcgacatct cggcccgcga agaagttgag gaagcgttgc
     3421 ggatgagcga gttgagatac cgtcaaatgt tcaacaacga ccctttcccc tctctcgtct
     3481 acgacatcga aaccctccag gtcgtcgacg tcaacgacat cgcgcttaca agttacggat
     3541 acagccgtga ggaattcctc tccctgacag tgatggacct cgtagcgtcc gagagtatcc
     3601 cggcgcttct cgacatcctc tccagacgtc ccaccagccg gtgcgcggct tcttcgcggc
     3661 acgtgaagaa ggatggcacc atcatcgatg tggaggtcac gggccatctg ctggacttcc
     3721 cggggaagca atatcgaatg ataaccgtta tcgatgtgac agagaggaaa atgacggagg
     3781 agcacctcaa tttcacccag gccgtggtcg accgcgtcgt ggactgcatc ttctggctcg
     3841 acagatatgc ccgcatcatc tacgtgaacg aagctgcgtg caggaccacg ggatactccc
     3901 gggatgagct cctctccatg accgtattcg acatcgatcc caccctgacg aaggaaggct
     3961 gggaatcgca ctggaaacag aagaaagcct gcgggtccct gctcaccgag tcctaccacc
     4021 gctcaaagga cggcagggtc tatcccgttg agatatcggg cagttatctt gcctatggaa
     4081 accgggaata caactgcacg ttcgtcaggg acatcacgga gcgcagaaaa gtggaccaac
     4141 aactgctgct cacgcagttc accgtggaca aggccgccgc catcatcctc tggatagggg
     4201 aagacgcccg gatactgtac gcgaacgacg aggcctgccg ctccacgggt tacacgcggg
     4261 aggatatgct gggcctcgct cttcccgtca tcgacccctc cttcgcgccc cccgtctgga
     4321 atgaccactg gaacgacgtt aaggagcggg gaaccatcat cttcgagtcg tttcagaagc
     4381 gcaaggacgg ctctctttac cccgtcgaga tcaagggaaa ctacatggaa tacggcggca
     4441 tcggctacat ctgctccatt gccttcgaca ttacggagag gaagaagacc gatgaacttc
     4501 tccgcatgac cagcttttcc atggaccaat cggcgatacc gacattctgg ctcgacatgg
     4561 acggcaatgc cttgcgcgtc aacaaggcgg cccttatttc cctcggatac acggaggagg
     4621 agatgctcgg caagaacgtc agaacctggg acgaggattt cccccatgac ctctgggaca
     4681 gccacatctt cccgaaactc aaagcaaggc attccctgac gatggaatcg aagtgcaaga
     4741 ggaagaacgg gagcaagttc cccgtggagc tgaacctgaa ctatatgaca tatgccggca
     4801 acgaatatgt
//
LOCUS       DAOPOA010000083         4789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_19410_length_4789_cov_3.565906, whole genome shotgun sequence.
ACCESSION   DAOPOA010000083 DAOPOA010000000
VERSION     DAOPOA010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19410_length_4789_cov_3.565906"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1262)
                     /locus_tag="PKJ17_02770"
     CDS             complement(<1..>1262)
                     /locus_tag="PKJ17_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOD74922.1"
                     /translation="QIITALALERSWTKEEILEAYINLVYYRGELQGVRAASKGLFGK
                     EPSGLTQLESVVLASLITSPNAPREKVAARAWRLALRAGMRITREEVGRKAADALASA
                     YRIAFDASLAPHVARMLLAGGRLKMATSLDGRLQRFALDALRDNLAALRGRNVADGAV
                     MVVDNATGEVLAYVGNSGSTSSAVWVDGIRAHRQAGSTLKPFLYGLAIEKGYLTASSL
                     LEDTPLSIMTPTGLYVPQNYDNQFRGTVSVRCALASSLNVPAVKTLLVTGLEPFVERL
                     KALGFASIKEEPEFYGYSIALGSADVELYELVGAYLALARGGRRTEMRLTFDGAASAG
                     TRVMDEKASYIVSRILSDRESRAVTFGLENPLATRYFSAVKTGTSKDMRDNWCIGFSR
                     RYTVGVWVGNFSGEPMRDVSGISGAAPL"
     assembly_gap    1263..1362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1363..1599)
                     /locus_tag="PKJ17_02775"
     CDS             complement(<1363..1599)
                     /locus_tag="PKJ17_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74923.1"
                     /translation="MFFRRLKGTGKTCAVAALVLLCLVAAGLLAVVRSVRNTDAGSFQ
                     ALKASYRTSDAVLLDRRGEVIHTLRIDDSGRRLEW"
     gene            complement(1604..>4789)
                     /locus_tag="PKJ17_02780"
     CDS             complement(1604..>4789)
                     /locus_tag="PKJ17_02780"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOD74924.1"
                     /translation="LDGNGTARTTIGDLPPSDVPREILSELEFRDPNGEIQTSSARIP
                     LYPSMLHAGIFTSGDVPADTLRYQVVVVDLKGKPQPGSEVSVRVFKRLTYSHRRRITG
                     GFYAFDNTTETKEIGEHCKGKTDANGILYCGGKAPATGRIVIEAEVKDSRGNVSSTNR
                     EVTVYGKDDTWYEARNDDRIDLIPRRRQVDPGETMSFEVRMPFRTATALVTVEREGVM
                     DAYITRVSRENPVIDIPVKPSYVPNVFVSALVVRGRLAGTQPTASFDPGKPAYRLGIA
                     EVSVGLKPHALKVKVDTDRKTYGVRQKVKARIAVRAPDGSAPPKGSEAALAVVDEGLL
                     ELKPNLSWGLLEAMMRRKGYQMSTATAQMMIIGKRHFGRKALPHGGGGGKQLTRELFD
                     TLVYWKSAVPLDDGGEADVAFDLNDSLTSFRVVAIASSGKDLFGTGAKTIRTTQDLMV
                     LSGLPGLVREGDRFTAGFTVRNTSARHMDVTVGLKLSGGKTSQPFAHLPLAIPAGQAR
                     EAAWQITVPTGAVKLDYEVSAKETSGESADTLKVAQKVVPAVSVRVIQATLTQVKDTL
                     KMDVERPAEAEPDRGGVAVTMRPRLAEGLDGVVDHMRRYPYVCLEQKTSRAVALQDRE
                     MWKDLMTQLPAYLDTDGLAKYFPLMMRGSDVLTSYLLSVSSESGYEIPQNVRARMVKG
                     LKGFVEGKVVRHSALQTADLSIRKMAAVAALARHGQSTAQLLTTMDIEPDLWPTSAVL
                     DWMDVLRRTPGVADVQVKLDKAARILRSRLNLQGTTMGLSTERSDQLWWLMVSTDLNC
                     ARTLIASMEIGTWGEDVPRIARGLVQRMKRGRWDTTTANAWGVLALQKFSRKYESAVV
                     KGTSSVTLGGKQDSLSWAENAKGGSLMLPWPGKRSVLTIDHRGEGRPWASVQSLAAVP
                     LKAPLASGYTIKKTITAVERKSPDVWTRGDVMRVQLQIDARSDMTWVVVSDPIPSGAA
                     ILGSGLGQDSALLSRQGRQRGWAWEAYRERSFEALRVYYEFVPRGKWTVEYSVRLNND
                     GTFRLPETRVEALYAPEMFGEIPNPPVEVRK"
BASE COUNT          809 a         1571 c         1392 g          917 t
ORIGIN      
        1 cacaggggcg ccgccccgct gataccgctg acatcacgca tcggctctcc cgagaagttg
       61 ccgacccaca ccccaacggt gtagcggcgg gagaaaccta tgcaccagtt gtcgcgcatg
      121 tccttgctgg tacctgtctt gaccgccgaa aagtagcgcg tggcgagagg gttctcgagg
      181 ccgaatgtga cggcacggga ctccctgtcg gacagtatgc gggagacgat gtaggatgcc
      241 ttctcgtcca tgacccgtgt cccggcactg gcggcgccat cgaaggtcag cctcatctcc
      301 gtccgccgtc caccccgcgc cagcgccaga taggccccga caagctcata gagttcgaca
      361 tccgccgacc cgagagcgat ggaatacccg tagaactcag gctcctcctt gatactcgcg
      421 aaccccagag ccttgagtct ttcgacgaag ggctcgaggc ccgtcacgag aagcgtcttg
      481 acggcgggca cgttgaggga cgatgcaagg gcgcacctca cactcacggt gccgcggaac
      541 tggttgtcat aattctgcgg cacgtagagc cctgtcggcg tcattatgga gaggggcgtg
      601 tcctcgagaa gggatgacgc ggtgagatag cccttctcga tggcaagccc gtaaaggaag
      661 ggtttcagcg tcgaaccagc ctgtctgtgt gcccggatgc cgtcgaccca cacagcgctc
      721 gatgtgctcc cgctattgcc gacgtaggca aggacctcgc ccgtggcatt gtcgacgacc
      781 atgacggcgc cgtcggccac attccgccca cgcaacgctg caaggttgtc cctgagcgca
      841 tcgagggcga acctctggag ccgcccatcg aggctggtcg ccatcttcag ccttccaccg
      901 gcgaggagca tgcgcgccac atggggcgcg agggacgcgt cgaaggctat gcggtatgcc
      961 gatgccaggg catccgccgc tttccttcca acctcctcgc gggtgatcct catgccggcc
     1021 ctcaacgcca gtcgccaggc cctcgcggcc accttttccc tgggcgcgtt gggcgatgtt
     1081 atgagagagg ccagaacgac ggactccaac tgggtgaggc ccgacggttc ctttccgaag
     1141 agacccttcg acgccgctct gacaccctgg agttctccgc ggtagtatac gagattgatg
     1201 taggcctcaa gtatctcctc cttcgtccag gagcgttcca gggcaagggc ggtgatgatc
     1261 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccactcca gccgcctgcc
     1381 gctgtcatcg atcctcagtg tgtggatcac ctcgccgcga cgatcgagaa ggacggcatc
     1441 ggaggtgcga tacgaagctt taagggcctg aaaggacccc gcgtcggtgt tcctgaccga
     1501 ccggaccacc gcgaggagcc cggcggcgac aaggcacagg aggacaagag ccgcgacggc
     1561 gcatgtcttc ccggtgcctt tcagccgtct gaagaacatc ctgctatttc ctcacctcca
     1621 cgggcggatt cgggatctct ccgaacatct ccggcgcgta gagcgcctcc acccgtgtct
     1681 cgggaagacg gaacgtaccg tcattgttga gcctcacgct gtactccacg gtccacttgc
     1741 cgcgggggac gaactcatag tacaccctca gggcctcgaa ggaccgttca cggtaagcct
     1801 cccacgccca tccccgctga cgtccctggc gggaaaggag ggcggaatcc tgcccgagac
     1861 cgcttcccag gatggcagcg cccgatggga tgggatcgct gacgaccacc caggtcatgt
     1921 ccgagcgggc atcgatctga agctgcacgc gcatcacatc cccgcgtgtc cacacatccg
     1981 gcgacttgcg ctccaccgcc gttatcgtct tctttatggt gtagcctgaa gccagcggcg
     2041 ccttcagcgg aaccgccgcc aggctttgca cgctagccca gggccgtccc tcgcccctgt
     2101 ggtcgatcgt aaggacgctc ctctttccgg gccagggcag catgaggctg cctcccttcg
     2161 cgttctcagc ccacgacagg ctgtcctgct tcccgccgag agttacggaa gatgtcccct
     2221 tcacgacagc ggactcgtac ttccgcgaga acttctgcag cgcgagaacg ccccaggcgt
     2281 ttgccgtcgt cgtgtcccag cggcccctct tcatcctctg taccaatccg cgggcaatgc
     2341 gcggtacgtc ctcaccccat gtgccgatct ccatggacgc gatgagggtc cgggcacagt
     2401 tgaggtccgt cgacaccatg agccaccaga gctggtccga tctctccgtg gagagcccca
     2461 tggtcgttcc ctgcaggttg aggcggctcc tgaggatccg ggccgccttg tccagcttca
     2521 cctgaacgtc ggctacaccc ggcgttcgcc tgaggacgtc catccagtca aggacggccg
     2581 acgtcggcca caggtcgggc tcgatatcca tcgtcgtgag aagctgcgcg gtggactgtc
     2641 catggcgcgc gagggccgcg accgccgcca ttttcctgat ggaaaggtcc gccgtctgga
     2701 gcgcggagtg tctcacgacc ttaccttcca cgaacccttt caggcccttt accattctcg
     2761 cgcggacatt ctgcggtatc tcgtatccgg actcgctgct caccgagaga aggtaggagg
     2821 tcaacacgtc actgccgcgc atcatcaggg ggaagtactt cgcgaggccg tcagtatcga
     2881 gataggcggg cagctgtgtc atgaggtctt tccacatctc gcggtcctgg agggcaacgg
     2941 ccctcgatgt cttctgttcg agacaaacgt agggatagcg gcgcatatgg tcgaccacgc
     3001 cgtcaagccc ctcggcgagc ctgggcctca tggtcacagc cacacccccg cggtcgggct
     3061 cggcttcggc ggggcgctcg acgtccattt tcagggtatc cttcacctgg gtcagcgtgg
     3121 cctgtattac cctcacgctt accgccggca ccaccttctg ggccactttg agcgtatcgg
     3181 ccgactctcc cgaggtctcc ttcgccgata cctcataatc gagcttcacg gcgcccgtcg
     3241 gcacggtgat ctgccacgcg gcctcccggg cctgccccgc cgggatggca agcgggagat
     3301 gtgcgaacgg ttgcgatgtc ttgccgccgc tcagttttaa tcccaccgtc acgtccatgt
     3361 gccgggccga cgtgttgcgc accgtgaatc ccgccgtaaa acggtcgccc tcccggacaa
     3421 gcccgggtag gcccgaaagg accatgaggt cctgggtggt ccttatcgtt ttcgccccgg
     3481 tgccgaaaag gtcctttccc gaactggcta tcgccacgac cctgaaggat gtaagagaat
     3541 cgttgagatc gaaggcaacg tcggcctcgc cgccatcgtc gagcggcacc gcgctcttcc
     3601 agtacacgag tgtatcgaag agttcccgcg tcaactgctt cccgccaccg cccccgtgag
     3661 ggagggcctt gcgcccgaaa tggcgcttgc cgatgatcat catctgcgcc gtggccgtcg
     3721 acatctggta gcccttccgg cgcatcatcg cctcgaggag tccccagctc aggttcggct
     3781 tgagttcgag gagcccctcg tcgacaacgg ccagggcggc ctcggaaccc ttcggcgggg
     3841 cggaaccgtc gggcgcccgc accgcaatgc gggccttcac cttttgcctt accccgtatg
     3901 tcttcctgtc cgtatcaacc ttgaccttga gggcgtgggg ctttaaaccc acgctcacct
     3961 cggctatgcc cagccggtag gccggtttcc cgggatcgaa ggatgcggtg ggttgcgtgc
     4021 ctgccagccg tccccggacc acgagcgccg acacgaagac gttcggcacg tagctaggct
     4081 tcacgggaat atctatgaca gggttctccc gggacactct tgttatgtaa gcgtccatca
     4141 ccccttcacg ttcgacggtc acaagggccg tcgccgtgcg gaaaggcatc cgcacctcga
     4201 agctcatcgt ttcgcccggg tcgacctggc ggcggcgggg gatgaggtcg atgcggtcgt
     4261 cgttgcgcgc ctcgtaccat gtatcatcct tgccgtaaac cgtgacctcc cggtttgtcg
     4321 aggacacgtt cccacgggag tccttcacct cggcctcgat cactatcctt cccgtcgcgg
     4381 gtgccttgcc cccgcagtag aggatgccgt tcgcgtcggt ctttcctttg cagtgctctc
     4441 ctatctcctt cgtttccgtt gtgttgtcga aggcatagaa accacccgtt atcctccgtc
     4501 tgtgggagta cgtcaaccgc ttgaagaccc tgacgctgac ctctgatccc ggttgaggct
     4561 ttcccttgag atcgacgacg accacctggt acctgagcgt atcggcaggc acatccccgc
     4621 tggtgaagat ccccgcgtga agcatcgaag ggtagagggg tatcctcgcc gacgacgtct
     4681 ggatctcccc gttgggatcc ctgaactcaa gttcgctcag tatctccctg ggaacgtccg
     4741 atggcggaag gtccccgatg gttgtccgcg cggtaccgtt cccgtcgag
//
LOCUS       DAOPOA010000084         4764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_19532_length_4764_cov_3.176258, whole genome shotgun sequence.
ACCESSION   DAOPOA010000084 DAOPOA010000000
VERSION     DAOPOA010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19532_length_4764_cov_3.176258"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="PKJ17_02785"
     CDS             complement(<1..145)
                     /locus_tag="PKJ17_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOD74925.1"
                     /translation="MSFSCGFIGLPNSGKSTIFNALTALAVPCQPYPFCTIDPNVGVV
                     PVPD"
     gene            complement(156..713)
                     /gene="pth"
                     /locus_tag="PKJ17_02790"
     CDS             complement(156..713)
                     /gene="pth"
                     /locus_tag="PKJ17_02790"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOD74926.1"
                     /translation="MHLLCGLGNKGSEYAFTRHNVGYLVIDRFSERFKIPVRKKDSGC
                     RIGSKDDLVLAKPDTYMNLSGGPIARLAGKLKVPPDRVIVIHDDMDMDFGKMRIRLGG
                     RDGGHKGVRSVIECLGGKDFWRLKIGIGRNPSMPAEEYVLSRFDGDEAGELADIIDIA
                     CDALKVFVFEGGSKAMSLFNRRGPE"
     gene            complement(720..1349)
                     /locus_tag="PKJ17_02795"
     CDS             complement(720..1349)
                     /locus_tag="PKJ17_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOD74927.1"
                     /translation="MEQVLIKADRRSATGKGVARKARAAGRIPAVLYGGGVDPVSITI
                     SSKDWDKITRHMKRNVILDMEIHGADSVESRPVMVKEVQRNGLGTEIMHIDFLQVSME
                     KTVEVEVSIHLKGKSKGEVLGGVIDVHLRSIKVECLPGQIPEEIVIDMTELDIGDSVH
                     VSDISLPGVKLIEHGDVAILSVVPPTVEEKRVATEEAAAEASEAKEKEE"
     gene            complement(1364..2299)
                     /locus_tag="PKJ17_02800"
     CDS             complement(1364..2299)
                     /locus_tag="PKJ17_02800"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOD74928.1"
                     /translation="MDKLRILTGNANPELAEKIGKYLGVKLARAKVNQFSDGEVQVSI
                     DESVRGMDTFVVQSTCPPVNHNVMELLVMLDALKRASAGRITVVIPYYGYARQDRKVL
                     PRTSISARLVANLITVAGASRILAMDLHAGQIQGFFDIPVDHLYALPVQFEYIKKIKG
                     DIVVVSPDAGGVERARELGKRINATIAIIDKRREKANESKVMHVIGDVKGRTAILIDD
                     MIDTGGTIVQAAQALMDNGAKVVYACCTHPVLSGEAIQRINNSPLKELIATNTIPLSP
                     AGKRSKKIKVLDVSPILGEAIKRIHSDASVSSLFI"
     gene            complement(2331..2402)
                     /locus_tag="PKJ17_02805"
     tRNA            complement(2331..2402)
                     /locus_tag="PKJ17_02805"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2368..2370),aa:Gln,seq:ttg)
     gene            complement(2411..2683)
                     /gene="spoVG"
                     /locus_tag="PKJ17_02810"
     CDS             complement(2411..2683)
                     /gene="spoVG"
                     /locus_tag="PKJ17_02810"
                     /inference="COORDINATES: protein motif:HMM:NF009749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOD74929.1"
                     /translation="MEITDVRIFPVDEKRVKAYASIVFDDCFIVRDLKVIQGDNKFFV
                     AMPSKKMKDGSFRDTVHPLNNATRQMIEESVLGAYELELSRAQEAL"
     gene            complement(2683..3540)
                     /gene="ispE"
                     /locus_tag="PKJ17_02815"
     CDS             complement(2683..3540)
                     /gene="ispE"
                     /locus_tag="PKJ17_02815"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOD74930.1"
                     /translation="MKTIVLSPAKVNLYLKVLSRRPDGYHDLRSLVDLISLHDTIHIE
                     DIAGDEIVVDDDRGILPRGEGNTVYRAVRMLREAAGVKKGVRVFIEKRIPIGSGLGGP
                     SSNAATVLRELAARWELGLSNEDLKGIGSRVGADVALFLHGGSCVMEGIGDVITPVRL
                     PSMWYVVVYPNVSVSTKAVYEGLRIVLTKKQNDIKLMENFSTPRQIAGILENDLEAVA
                     ITLCPQIQGIKDALKGTKTLGSLMSGSGSSVFAVFENEQDASQASLLSAIRKMGTVFV
                     AQSVQGGVN"
     gene            complement(3546..>4764)
                     /locus_tag="PKJ17_02820"
     CDS             complement(3546..>4764)
                     /locus_tag="PKJ17_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOD74931.1"
                     /translation="HFEVGERIEGVLEDGQELIVQVSREPIGQKGTRVTSKITLPGRL
                     LVLMPGTDHIGVSRKIEDEEERKRLATVMREICPKGFGLIGRTASEGKTADELATDLN
                     FLKRIWESIQEKAKATRAPAILHQELGIIFRVIRDLHSHSLKRIIVDDQYIHSRLEGF
                     LKEYLPEEGCEVIHFDERDPIFEVYGIEMEVAKLLQKKIWLKSGGYIVLDYTEALTVI
                     DVNTGRYLGKKDLEDTILRTNLEAVKEIAYQIRLRNVGGIIIVDFIDMERKESRETVF
                     QALMDTLKKDRIKTFAYPISELGLVQLTRKRTRHNIVNLLTETCPNCEGSGYVKSRHT
                     VCYEVLRELRSSCRKGEGRVFNIYLSPDVADLMYEEERTSLEHLETTYGTKVNLIANP
                     DFAIDKFQIEGIM"
BASE COUNT          941 a         1447 c         1220 g         1156 t
ORIGIN      
        1 cgtcgggaac ggggacaaca cccacgttgg gatcgatggt gcagaaagga tagggctgac
       61 acggcactgc cagtgcggta agtgcgttga atatggtcga tttccccgaa ttgggaagac
      121 ctatgaaacc gcacgaaaaa ctcatgtcct tcgactcact ccggtccgcg ccggttaaag
      181 aggctcatcg ccttggaccc gccttcgaag acgaagacct ttaaggcatc gcaggcgatg
      241 tcgatgatat ccgcgagctc cccggcctca tccccgtcga accgtgacaa cacgtattcc
      301 tcggcaggca tcgagggatt cctccctatg cctatcttga gccgccagaa gtccttgccc
      361 cccaggcact ctatcaccga ccgcactccc ttgtgaccac cgtctctgcc tccgagacgg
      421 atccgcatct tgccgaaatc catgtccatg tcgtcgtgga taacgatcac gcggtcgggc
      481 ggcaccttca gttttcctgc cagtctggcg atggggccgc ccgagaggtt catgtacgtg
      541 tcaggcttcg cgaggaccag atcatccttc gacccgatcc tgcaacctga atctttcttc
      601 cgaaccggta tcttgaaacg ttccgaaaaa cggtcgatga cgagataccc gacgttatgc
      661 cgcgtgaagg cgtactcgga gcctttgttg ccaagtccgc acaataaatg cacggacctt
      721 tactcctctt tctccttcgc ttcggaagcc tcggccgcgg cttcctctgt cgccaccctc
      781 ttctcttcca ctgtaggagg cacgacactg agaatggcca catctccatg ctcgatgagt
      841 tttacgccgg gaagggatat atcgctcacg tggacggaat cgccgatatc gagctccgtc
      901 atgtctatga cgatctcttc cggtatctgg ccgggaaggc actccacctt gatggacctc
      961 agatggacat cgataacgcc gccgaggacc tcgcccttgg acttgccctt caggtggatg
     1021 gagacttcca cctcgaccgt cttctccatg gagacctgga gaaaatcgat gtgcatgatc
     1081 tcagtaccca gaccgttcct ctgcacctct ttgaccataa cgggcctgct ctcgacgcta
     1141 tcggcaccat ggatctccat gtcgagaatg acgttcctct tcatgtgcct cgtgatcttg
     1201 tcccagtcct tcgatgagat cgttattgaa acgggatcaa caccgccccc gtagagcacc
     1261 gccggaatcc tccctgccgc cctggccttt cgtgcaacac cttttcctgt tgcgcttctt
     1321 ctgtctgcct tgatcaaaac ctgttccatg atgttcctcc caactatatg aacaacgaac
     1381 ttacggacgc gtcgctgtgt attctcttta tggcctctcc gagaataggc gatacatcca
     1441 acacttttat cttcttcgat ctcttcccag ccggagaaag gggaatggta ttcgtcgcta
     1501 tcagctcctt gaggggtgag ttattgatcc tctggatcgc ctctcccgag agcacgggat
     1561 gcgtgcagca tgcatagacg acctttgctc cgttatccat gagggcctgg gccgcctgga
     1621 cgatcgttcc gcctgtgtcg atcatgtcat cgatgaggat ggcagtcctg cccttcacgt
     1681 cgccgataac atgcataacc ttggattcgt tggccttttc cctccgcttg tcgatgatag
     1741 cgatggtggc gttgatcctc ttgcccagtt cgcgggcccg ttccactccg ccggcatcgg
     1801 gagaaaccac cacgatgtcc ccctttatct tctttatgta ctcgaactga acggggagtg
     1861 cgtaaagatg gtcgacggga atgtcaaaga acccctgtat ctggcccgcg tggaggtcca
     1921 tggccagtat cctcgaagct ccggccacgg ttatgaggtt cgccacgagc ctcgccgaga
     1981 tggacgtacg aggaagcacc tttcggtcct gcctggcata cccgtagtag ggtatcacca
     2041 cagttatcct gcccgcggag gccctcttaa gcgcgtccag catgacaaga agctccatca
     2101 cgttatggtt caccggcggg cacgtggact ggacgacaaa ggtgtccata ccgcggacac
     2161 tctcgtcgat ggagacctgg acctcaccgt cgctgaactg gttcaccttg gccctggcca
     2221 gcttgacccc aaggtatttt ccaatctttt ctgctagttc ggggtttgcg ttgcctgtca
     2281 atattctgag tttatccaat caaaaccctc ctgagagttg tatgcgtatt ctggggcggg
     2341 aggattcgaa cctccgaatg caggctccaa aggcctgtgt cttaccgctt gacgacaccc
     2401 caaatgattg tcacagtgct tcttgagccc tgctcaactc cagctcatag gcgccgagaa
     2461 cgctctcctc gatcatctgt cgggtggcgt tgttcagagg gtgaaccgta tcccgaaatg
     2521 agccgtcctt catcttcttg ctcggcatgg cgacgaagaa tttgttgtca ccctggatca
     2581 ccttaagatc gcggactatg aaacaatcgt caaagacgat cgatgcatac gccttcaccc
     2641 gcttttcgtc cacggggaat atccttacat ccgtaatctc cattagttta cccctccctg
     2701 cacgctctgc gcaacaaaaa cagtacccat cttccgtatt gccgataata atgatgcttg
     2761 acttgcgtcc tgttcattct cgaagacagc gaacaccgat gagccgcttc cactcatcag
     2821 cgaaccgagg gtctttgtcc ctttcagtgc atcctttata ccctggatct gcggacatag
     2881 cgttatggcg acagcttcca gatcgttttc cagaatgccc gcaatttgtc ttggagtact
     2941 aaaattttcc atcaatttaa tatcgttttg cttctttgtc aacacgattc tcaggccctc
     3001 gtagacggcc ttcgtcgata cactgacatt cggatagact acgacgtacc acatgctggg
     3061 aaggcgcacc ggagtgatca catcgcctat gccttccatg acgcaggacc ctccatggag
     3121 aaagagggcg acatccgcac ccacccgact gccgataccc ttgagatctt cgttgcttaa
     3181 ccccagttcc caccgcgcgg caagctccct caagaccgtc gcggcgttgc tgcttggacc
     3241 gccaagcccg ctgcctatgg gtatccgctt ctcgatgaag acacgcactc ccttcttcac
     3301 tcccgcggcc tcccgcagca tccgaacagc gcggtagacc gtgttcccct cgccccgggg
     3361 aaggatgccc ctgtcgtcgt caacgactat ctcgtccccg gcaatgtcct cgatgtgtat
     3421 ggtgtcgtgc agggagatga ggtcgacgag gcttcggaga tcatggtatc cgtccggtct
     3481 tcgggaaagg accttgagat agaggttcac cttggcggga gaaagaacaa tggtcttcac
     3541 ggctgctaca ttatcccttc tatctggaac ttgtcgatcg caaagtcggg attggcgatg
     3601 aggttcactt ttgtcccgta ggtggtctcc agatgctcga gggaagtcct ttcctcttca
     3661 tacatcaggt cggccacgtc gggagagaga tagatgttga agacccgccc ttcgcctttc
     3721 ctgcaggaac tcctcagttc tctgaggacc tcgtagcaga cggtgtggcg ggacttgacg
     3781 tatcccgacc cttcgcagtt gggacaggtc tcggtgagga gattgacgat gttgtggcgc
     3841 gtgcgctttc tcgtgagctg aacgagcccc aactcgctga tgggataggc aaaggtcttg
     3901 atcctgtcct tcttgagcgt gtccatcaac gcctgaaaga ccgtctcgcg ggattccttg
     3961 cgctccatgt cgatgaagtc gacgatgatg atgccaccca cgtttcgcag ccgtatctgg
     4021 taagctatct ccttgaccgc ctccaggttg gtcctcagga tggtgtcttc caggtccttc
     4081 ttgccgagat accgtcccgt gttcacatca atgacggtga gggcttcggt atagtcgaga
     4141 actatgtacc cccccgactt cagccatatc tttttctgga gaagcttggc cacctccatc
     4201 tcgatgccat agacctcgaa tatggggtca cgctcatcga aatggatcac ctcgcagccc
     4261 tcctcgggaa ggtattcctt gagaaacccc tccaggcggc tgtggatgta ctgatcgtcg
     4321 actatgatcc tcttgaggct gtgggaatgg agatcgcgga tgacccggaa gatgatgccg
     4381 agctcctggt ggaggatggc cggcgcgcgt gtggcctttg ccttttcctg gatgctctcc
     4441 cagatgcgct tcaggaagtt gaggtccgtg gcgagttcgt ccgccgtctt gccctccgac
     4501 gccgtcctgc ctatcaaccc gaaccccttc gggcatatct ccctcatcac cgtggcaagg
     4561 cgcttgcgtt cctcctcatc ctcgatcttc cgggagacgc cgatgtggtc cgtgccgggc
     4621 atgaggacca ggagccgtcc gggaagggtg atcttcgatg tgacacgcgt ccccttctgc
     4681 ccgataggct cgcgggagac ctgaacgata agctcctgcc cgtcctcgag gacgccctcg
     4741 atcctctccc ccacctcgaa gtga
//
LOCUS       DAOPOA010000085         4748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_19609_length_4748_cov_3.013424, whole genome shotgun sequence.
ACCESSION   DAOPOA010000085 DAOPOA010000000
VERSION     DAOPOA010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19609_length_4748_cov_3.013424"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1014
                     /locus_tag="PKJ17_02825"
     CDS             73..1014
                     /locus_tag="PKJ17_02825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOD74932.1"
                     /translation="MRFVLALSFLVSLFLAPYALCAQGENVGITAVSYILVEKDTFQI
                     ITGRDYHRRLPPASTTKVMTALVAMERLGGDEIIVPDKQVLQISPSKMSLVPGKKYRS
                     EDLMKGTMVKSANDAAYALATYVSGSEGAFARLMTQRAREIGAIDTNFENASGLPSDN
                     QYSTPYDLALIFRHAMANERFVELIATRYFLFEDSTRKVRYKNHNRLLFCFEPAIGGK
                     TGYTRAARHCYVGAFEKGGRVYILALLGSRSLWGDTVEILKTIYDDVPTDKELRQARA
                     SSVTLCSTTAPKKKAASRKKVVKHRTMKSKKKTVKKQ"
     gene            1011..2336
                     /locus_tag="PKJ17_02830"
     CDS             1011..2336
                     /locus_tag="PKJ17_02830"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOD74933.1"
                     /translation="MKRCVAIVTAFLFLAASAGAFALTNEEERQYGKEIYLEIARSAP
                     INNDPYISLYLNEIRVRLESKTTMPFPVVLTVIDSPTIDAFATIGGYVYIAAGLIANC
                     DDEEEVAGVMAHEFAHIKKRHIAKRMGKQKYLNATMIATMLAAMLVGDSAREAVLVTG
                     MGTAQSMALKYSREDEDEADREGSILANEAGYGGLGIADFLKKLRAGGGDKLYPQYLL
                     THPYHESRIINLEKMWTRKPPSVDTSLFPYIIARAKVVQDYRSRGLADDIWVRRYARD
                     SKDPVAAYGAALYHSLKGNFEEAVRIARTINSPYRSVFLGEIYVTASRFPEAAGVLKD
                     ATEKVGRYYLARAYEGMGRTDLAATTYRSLIPYAPSYPEIYYRLGMIEGRTGNEAAGH
                     ANLGRYYMYKGNYMLAKTNFEKAISRYGINSRESTELMRLLDSLEEKKK"
     gene            complement(2360..3280)
                     /locus_tag="PKJ17_02835"
     CDS             complement(2360..3280)
                     /locus_tag="PKJ17_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOD74934.1"
                     /translation="MQGIGSQPLAAMSTGDEVLVVHLGGLGDVCLSESVFLSLRGHFG
                     DRLVGLGNRRFFDLFGGYFLRTHGVESRHWLYLFSEKLTGPRWGRIAFIGKDRRGSIR
                     SRWKAHSVEEPLFIDMYPEGAFGSPSPGDGTAAAGETVHVEDYQLRQLSAVGIEPARK
                     EIVPKEARRVILYAEQGFSKEKWPVENFVVLREVLEEAGIDVRLMRPPELSLSTRGSI
                     FFEDLADVKDFFGIGGVFVSNDSGMAHLAGVCGLTTMTIFTAFDPAVWHPRGRNISLR
                     TGVDRTDVESLAGMIIGIMERWDGKGEGLS"
     gene            complement(3952..>4748)
                     /locus_tag="PKJ17_02840"
     CDS             complement(3952..>4748)
                     /locus_tag="PKJ17_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOD74935.1"
                     /translation="DVGIVATVGSDFDMEGISFLRDRSVDMSGIEVDHGKTFRWEGRY
                     GYDLNQAITLKTELNVIETFKPKVPEDYRNAEFLFLANIDPELQLHVIEQVNKPVALV
                     LDTMNFWIENKYEALMKVIGRVDMLVINEAELREITKEHNLLKGARKLIELGPVCIVV
                     KRGEYGVFMMNRDEVFLAPAYPLEDVFDPTGAGDTFAGGFMGYLANVGEATSETVKQA
                     IIMGTVMASFNVEDFSINRIKRLTMPEIKQRYHALKGCMRFGDISL"
BASE COUNT         1030 a         1423 c         1385 g          910 t
ORIGIN      
        1 acttaacact taaaacttaa aacggtttct acggttgtaa gggttgtcca aatagtgtat
       61 atataagcaa cgatgcgttt tgtacttgcc ctttccttcc tggtttccct tttcctcgca
      121 ccttatgccc tttgcgccca aggggagaac gtcggcataa cggcggtgtc ctacatactt
      181 gtcgagaagg acaccttcca gatcatcacg ggcagggatt atcaccgcag gcttcctcca
      241 gcgagcacga ccaaggtgat gacggcgctc gtcgccatgg aacgcttggg cggcgacgag
      301 atcatcgttc ccgacaagca ggttctccag atatccccgt ccaagatgag cctcgttccc
      361 ggaaagaagt accgttccga ggacctcatg aaggggacaa tggtgaaatc ggccaacgat
      421 gcggcatatg ccctcgcgac ctacgtcagc ggctccgaag gcgcctttgc ccggctgatg
      481 acacagcggg cacgggagat cggcgccatc gacacgaact tcgagaatgc ctcaggtttg
      541 ccttcggaca accagtacag caccccctac gacctggccc tcatattccg gcatgccatg
      601 gccaacgagc gcttcgtcga gctcatcgcc acgcggtact ttctgttcga ggactccacg
      661 cgcaaggtgc gctacaagaa ccacaacagg ctgctctttt gcttcgaacc cgccatcggc
      721 gggaagacgg ggtatacccg ggcggcgaga cactgttacg tgggggcctt cgagaagggc
      781 ggcagggtct atatcctggc cctgctgggg agccgcagcc tctggggcga caccgtggag
      841 atactgaaga caatatacga tgacgttccc accgataagg aactccgcca ggcgcgggcg
      901 tcctccgtca cgctctgttc cacgacggct ccgaagaaga aagcggcatc ccggaaaaag
      961 gtcgtcaagc accgtaccat gaagtcgaag aagaagacag taaagaaaca atgaaaagat
     1021 gcgtagccat cgtaacggca tttctcttcc ttgcagcgtc cgcgggtgct ttcgccctca
     1081 ccaacgagga ggaacgccag tacggcaagg agatctacct ggagatcgcc cggtcggcgc
     1141 cgatcaacaa cgatccctac atctcgctgt acctcaatga aattcgggta aggcttgaaa
     1201 gcaagacgac catgcccttc ccggtcgtcc tcaccgtgat cgattccccc accattgacg
     1261 cctttgccac gatcggcgga tatgtctaca tcgcggcggg tctcatcgcc aattgcgacg
     1321 acgaggaaga ggtcgccggc gtcatggccc atgagtttgc ccatatcaag aaaaggcata
     1381 tcgcgaagag gatgggcaaa cagaagtatc tcaacgccac catgatcgcc accatgctcg
     1441 ccgcgatgct cgtcggtgat tcggcacgtg aggccgtcct tgtgacgggc atgggcacgg
     1501 cgcagtcgat ggccctgaag tactcccgcg aggacgagga cgaggcggac cgcgagggtt
     1561 ccatccttgc caacgaggcg ggctacggcg gtctcggcat tgccgacttt ctcaagaagc
     1621 tccgggccgg cgggggcgac aagctctatc cccagtatct tctcacccac ccctatcacg
     1681 aaagccgcat catcaacctg gagaagatgt ggaccaggaa acccccttcc gtggacacct
     1741 ctctctttcc ctacatcatc gcgcgggcga aggtcgtgca agactaccgt tcccggggcc
     1801 ttgcggatga tatctgggtc aggcggtacg cccgggacag caaggatccc gtcgcggcat
     1861 acggcgcggc gctgtatcac tccctgaagg gcaattttga agaggcggtg cggatcgccc
     1921 ggaccatcaa ctctccttac aggagcgtct tcctgggaga gatctacgtc actgccagca
     1981 ggtttcccga ggccgccggg gtgctgaagg acgccacgga aaaggtgggc cgctactatc
     2041 tcgccagggc atacgagggc atgggcagga ccgatcttgc cgcaaccacc taccggtccc
     2101 tcatccccta cgccccatcc tatccggaga tatactaccg tctcggcatg atcgagggca
     2161 ggacgggcaa cgaggccgcg ggccacgcca acctgggccg gtactacatg tataagggca
     2221 actacatgct ggcgaaaacg aatttcgaaa aggccatctc ccgctacggc atcaattcac
     2281 gggagagcac ggaactcatg cgcctcctcg acagcctgga agagaagaaa aagtagaata
     2341 aggggtccgg gggcaggatt caggaaagac cctctccctt tccatcccat cgttccatta
     2401 tcccgatgat catcccggcg agcgactcga catcggtcct gtcgacgccg gttctcagcg
     2461 agatgttcct tcccctggga tgccacacgg cagggtcgaa ggcggtgaag atggtcatcg
     2521 tggtcaggcc gcagacaccg gcaagatggg ccatgccgga atcgttggac acgaagacgc
     2581 ctccgatgcc gaagaagtcc tttacgtcgg cgagatcttc gaagaaaatg gacccccggg
     2641 tggaaaggga cagctcgggc ggcctcatca gacgcacgtc aatgccggcc tcctcaagca
     2701 cctcccggag gacaacgaag ttctccacag gccacttctc cttcgaaaac ccttgctcgg
     2761 cgtagaggat gacccgtcgc gcctctttcg gaactatctc cttccgcgcc ggctctatcc
     2821 ccacggcaga gagctgccgc aactggtagt cttcaacgtg cacggtctcc ccggcggcgg
     2881 cagtcccgtc gcccggggaa ggtgagccga atgccccctc ggggtacatg tcgatgaaca
     2941 gcggctcctc gacggaatga gccttccagc ggctgcggat ggaaccccgg cggtccttgc
     3001 cgataaaggc gatgcgaccc cagcgcggcc ccgtgagctt ctcggagaag aggtagagcc
     3061 agtggcgtga ttccacccca tgggttctga gaaagtagcc tccaaagagg tcgaaaaaac
     3121 gtctgttgcc caggcccacg aggcggtccc cgaaatgccc gcgcagggag aggaagacgg
     3181 attctgagag acatacatcg ccaaggccgc cgagatggac gacgaggacc tcgtccccgg
     3241 tcgacattgc cgcgaggggt tgcgacccta ttccctgcat atcgctaacc aacggtccct
     3301 gtgatgctgc ggttcatatc gaagatggcg gccctggttt tcatattatg tgcaccattg
     3361 taacgggaaa agcgagggtc gataaaggaa aaagatcgcg cgaggcgggt gagtcagacc
     3421 cgccccgcgc ttgaacgtgc tcttcgcacg tgggggtagt cgcagcgcgc gtccaccaac
     3481 aatacgaact cccggcctcc ataggggcca atctcgcggg gaacggcacc acacggtgtc
     3541 gccgcgcttc ttcacgggcg cacacccggt ccgcggccgc cgcggacata cgcttgagag
     3601 tgcattgcct ggttttcggt ttgctaagta ataaccattt tcccggagcc aggtcaactg
     3661 caaagaacgg attgccagga aaggggacag atgagggcgg gggcaggata ggaccaatgg
     3721 gacgatcgta ggacgaatag gacatatagg gcccatagga cgtatgtgaa ggggtgggcg
     3781 ctacccggac aaacgagagg ggttgcggcg aggagcgggt tatgggtgaa aaaaagaacc
     3841 ccttatctta ccttgacagc gcaaagcaaa ataaggggat ctatgatcta ctttcttctc
     3901 tcgcccctaa ccctcaagcc attcaagcgg ctcaagcggt tcaggccgcc cttacagact
     3961 tatgtctccg aaccgcatgc agcccttcag ggcgtgatac ctctgtttga tctcgggcat
     4021 ggtgagcctc ttgatcctgt tgatgctgaa gtcctcgacg ttgaaggagg ccatgacggt
     4081 gcccatgatg atcgcctgct tcaccgtctc cgaggttgcc tcgccaacgt tggcgaggta
     4141 gcccatgaag cccccggcga aggtgtcgcc ggcgcccgtg gggtcgaaga cgtcctccag
     4201 cggatacgcg ggggcaagga agacctcgtc gcggttcatc atgaagacac cgtattcccc
     4261 gcgcttgacc acgatacaca cggggccgag ctcgatgagc ttccgggcgc ccttgaggag
     4321 gttgtgttcc tttgttattt ccctcagctc cgcctcgttg atgacaagca tgtccacccg
     4381 gcctatcact ttcatgaggg cctcgtactt gttctcgatc cagaagttca tggtatcgag
     4441 gacgagggcg acgggtttgt tgacctgttc gatgacgtgc aactggagct cggggtcgat
     4501 attcgcgagg aagaggaatt ccgcgttcct gtaatcctcg gggaccttcg gtttgaaggt
     4561 ctctatgacg ttgagctccg tcttgagcgt tatggcctgg ttgaggtcgt atccgtacct
     4621 gccctcccag cggaaggtct tcccgtggtc cacctctatc ccggacatgt cgacgctgcg
     4681 gtccctgagg aaggatatgc cctccatatc gaaatcgctg cccaccgtgg ccacgatgcc
     4741 gacgtcgg
//
LOCUS       DAOPOA010000086         4713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_19773_length_4713_cov_3.701374, whole genome shotgun sequence.
ACCESSION   DAOPOA010000086 DAOPOA010000000
VERSION     DAOPOA010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19773_length_4713_cov_3.701374"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PKJ17_02845"
     CDS             <1..797
                     /locus_tag="PKJ17_02845"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD74936.1"
                     /translation="RDLYIWKKDWDGAYDVEVKIKRHVKTDEETRRLVGIRYEKVCTL
                     FNERFSANADRIIDELKEIIGEDKRFIPAYILLGEAYKKTGKLNEAGRVYGRGYTKTG
                     HVEFLLKMEDLYIDRGDPEVILKIYRRVLDVSPEDHLISFLYARLCLRLEMIDEAIDT
                     LNTLIAEGEDFKGLHRAMAEAYIHRGELENAVEEFRGAFPIEQQVYIPFVCTKCQAIK
                     EEWSDFCESCYSWNTINVKKEEFLHADMNELRMLYDEAEWTRGEPS"
     gene            794..1990
                     /locus_tag="PKJ17_02850"
     CDS             794..1990
                     /locus_tag="PKJ17_02850"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOD74937.1"
                     /translation="MMDELLLSNDNKVIFLILDGLGDIPNPDFGLMTPLEAAAKPNMD
                     HLATKRGVLGRIIPVGTGITPGSGPGHLSLFGYDPVASEIGRGVLEVLGLNMDLRNGD
                     LAARANFCTIREDVVTDRRADRIPTEETERLCAMISASVKDIEGVEVIIRPGKSHRFA
                     AIFRGAGLSDKLTDADPHKDNKPFAYTTEKAKGAEFAARVVNGFIDRVRDLLKAEPVA
                     NGALLRGFSQKPDITPFAKRYRMKALAIATYPMYRGIAKVLGMDVKEEPGSYEEAVDI
                     LKKNYNDYQFFFFHVKETDLAGEDGNFKEKVKAIEDVDRFLPGISALDPQALVVTGDH
                     STPCPLKGHSWHPVPLLIVTKTGETDGLAFHEKNCIVGSAGTIYSKELMSLALAHGGR
                     LDKYGA"
     gene            1983..2675
                     /locus_tag="PKJ17_02855"
     CDS             1983..2675
                     /locus_tag="PKJ17_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOD74938.1"
                     /translation="MLSLFNCALALFYPESCVVCGADGSVLCPRCIDDLRTVKETEVC
                     PVCGRWLGRRIQCAVCSGAEMGFSRGIYGFYYERGLREAVHAFKFRGRKDVGRRLARL
                     AGEKVRPLRGAFDRIVPVPVSGRRLRERGFNQSYIISEEISSIAGGTVDHRALVKRGG
                     TRDQFTLSRRERKSNVRGAFHVRDRSSVEGKRVLLVDDLFTTGYTASEAARTLKAAGS
                     TDVTLFALARTP"
     gene            2675..>4713
                     /locus_tag="PKJ17_02860"
     CDS             2675..>4713
                     /locus_tag="PKJ17_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74939.1"
                     /translation="MKKVVVVLSVLVALVVLGIVIVTYNLPAVLSHLLTRSTGMSVRI
                     ERADLSFSDGTVALTLGNTRFKGPVSGKVGTLAARMWFSGGMILDRLTIKDFDVVVGK
                     VEMTKGGFSTTIGLLEVSNGTVIVGDRKLVIGSIVAENINTKKPLRFVASITDPDHAG
                     KVRVVGGSIVENGKHRVKGSVEVDAFGLEKIDPILNGVVNGKGEFVFYEGALTLTGKC
                     DSPKLTIRDTWLKKALVVNKVTARSTITTKGPDVHIAVYDTKYRDAPFTIDVSMKNLL
                     FSRLDITSGSIPMSAVREYLMMDEAGYDVWAYVRDGFLKIRKITYEKKKPFIAELELK
                     GLTGEYEGKRLTDISGVLEMIDNKGTLSEGKGFFKATSFHDLKGTMEFGKKPRIQLTG
                     KYVIDLQHVGEFVDMRGVVVQKGTAEGTVEVDSGQGKHPLKLGGSGRIKGAEISWKGQ
                     SFTVDGPFRLAGREMTFDPIVVTGRETQVTMQGKWGPQGLTTSLKGYIDPGLPGRFAG
                     KTVRASGKAVVDARVTVADGQIGVNGNANMDDVIFAVPGFIRKTKGVQSRAAVKFTRK
                     KTGEIVVDDLSCNLDTINVRASGKISGDGGIESRVTLRAKDTGRAASLFSLGEDIRGG
                     DVSIDLAVSDLRFPLTKLPWVVGSASMKKGFMKVPGIPKVLANIDLRAGFRGHEF"
BASE COUNT         1056 a         1317 c         1473 g          867 t
ORIGIN      
        1 ttcgggacct ctacatatgg aagaaggact gggacggcgc ctatgacgtg gaagtgaaga
       61 tcaagcgcca cgtcaagacc gatgaagaga cgagacggct tgtcggcatc cgctacgaga
      121 aggtgtgcac cctcttcaac gaaaggttct ccgccaatgc ggacaggatc atcgatgagc
      181 tcaaggagat cataggcgag gacaagcgct tcataccggc ctacatcctc ctcggggagg
      241 catacaagaa gacgggcaag ctcaacgagg ccggccgggt ctatggaagg ggatacacaa
      301 agacgggcca cgtggagttc ctgctgaaaa tggaagacct ctacatagac aggggcgatc
      361 cggaggtcat cctcaagatc tatcgcaggg tcctcgacgt gtctcccgag gaccacctga
      421 tctcctttct ctacgcgcgg ctctgcctgc gcctcgagat gatcgacgag gcgatcgata
      481 ccctcaacac cctcatagcc gagggcgaag acttcaaggg tcttcaccgg gcgatggcag
      541 aggcctatat ccaccgggga gaactggaga acgccgtcga ggagttccgg ggcgccttcc
      601 cgatagagca gcaggtctac atacctttcg tctgtaccaa gtgtcaggcc ataaaggagg
      661 agtggagcga cttctgcgag agctgttaca gctggaacac gataaatgtg aagaaggagg
      721 aattcctcca cgccgacatg aacgaactgc gcatgctcta cgatgaggcg gaatggaccc
      781 gaggtgaacc atcatgatgg acgagcttct cttatcgaac gacaacaagg tcatttttct
      841 catacttgac ggtctcggag atatcccgaa ccccgatttc ggtcttatga cccctctcga
      901 agccgctgcc aagccgaaca tggaccatct tgccacgaaa aggggcgtgc tcggcaggat
      961 catacccgtc ggcacgggta tcacgcccgg cagcggtccg gggcacctca gcctcttcgg
     1021 gtacgatccc gtggccagcg agatcggccg cggcgttctc gaagtcctcg gtctcaacat
     1081 ggacctgcgt aacggggacc ttgcggcacg ggccaacttc tgcaccatca gggaagacgt
     1141 ggtgaccgac cgcagggcgg acaggatccc gacggaggag acggaaaggc tctgcgccat
     1201 gatcagcgcc tcggtgaaag acatcgaagg cgtcgaggtc atcatcagac cggggaagtc
     1261 ccaccggttt gccgccatat tcaggggcgc gggtttgagc gacaagctta ccgacgccga
     1321 cccccacaag gacaacaagc ctttcgcata taccactgag aaggcgaaag gcgcggaatt
     1381 cgccgcccgt gtggtgaacg gtttcatcga cagggtcagg gacctgctca aggccgagcc
     1441 ggtggccaac ggggcgctgc tccgcggttt ctcccagaaa ccggacataa cacccttcgc
     1501 gaagagatac aggatgaagg cgctcgccat cgcgacgtac ccgatgtacc gggggatcgc
     1561 gaaggtcctc ggtatggatg tcaaggagga gccgggcagt tacgaggagg ccgtggacat
     1621 cctgaagaag aactacaacg actaccagtt ctttttcttc cacgtgaagg agacggacct
     1681 tgccggcgag gacggcaatt tcaaagagaa agtgaaggcg atcgaggacg tggacaggtt
     1741 ccttcccggg atatcggccc tcgatcccca ggcgctcgtc gtaacgggcg accactcgac
     1801 gccctgtccg cttaaaggac acagctggca tcccgttccc cttctcattg ttacgaaaac
     1861 gggtgagacc gacggacttg cctttcacga gaagaactgc attgtcggga gcgcgggaac
     1921 catatacagc aaggaactca tgtctctagc ccttgcccac ggagggcggc tcgacaagta
     1981 cggtgcttag ccttttcaac tgcgcgttag ccctcttcta ccccgaatcc tgtgttgtct
     2041 gcggcgcgga tggcagcgtc ctttgcccac gatgtatcga cgatctgagg accgtcaagg
     2101 agaccgaagt gtgccctgtg tgcggtagat ggctgggaag gcgcattcag tgcgcggtct
     2161 gcagcggggc ggagatgggg ttctcccggg gcatctacgg tttctactac gaaaggggcc
     2221 tgcgggaagc ggtccacgcc ttcaagttcc ggggcagaaa ggatgtcggc aggcggcttg
     2281 cccgactggc cggggagaag gtgcgcccct tgaggggcgc cttcgaccgt atcgtgcccg
     2341 ttcccgtttc cggcaggcgg cttcgcgaga gaggctttaa ccagtcctac atcatctccg
     2401 aggaaatatc gtccatcgcg ggtggaaccg tcgaccaccg ggcgctcgtc aagcgcggcg
     2461 gtacccggga ccagttcacc ctgtcccggc gggagcgcaa gagcaacgtg agaggcgcct
     2521 ttcacgtgcg cgacaggtcg tccgtcgaag ggaaacgcgt ccttctcgtc gacgacctct
     2581 tcacgacggg atacacggcc tccgaggccg ctcggaccct caaggcggcg ggctcgacgg
     2641 acgtcacgct tttcgccctg gcaaggacac cgtgatgaag aaggttgttg tcgtgctctc
     2701 cgtgttggtt gcgctcgtgg tcctcgggat cgtcatcgtg acctacaacc tgcccgcggt
     2761 cctgtcccat ctccttacgc ggtcgacggg aatgtccgtc aggatcgaaa gggcggacct
     2821 gtctttcagc gacggcacgg tggccctgac actggggaac acccggttca agggacccgt
     2881 ttcgggtaag gtcgggaccc tggcggcacg catgtggttc tcggggggga tgatcctcga
     2941 ccgcctgacg atcaaggatt tcgacgttgt cgtcggcaag gtcgaaatga ccaagggagg
     3001 tttctccacc accatcggac tccttgaggt ctcgaacggt accgtgatcg tgggagatcg
     3061 caaactggtc atcgggtcga tagtcgccga gaacatcaac acgaaaaaac ctttgcgttt
     3121 cgttgcgtcg atcaccgacc ccgaccatgc cggcaaggtc agagtcgtcg ggggcagcat
     3181 tgtcgagaac ggaaaacacc gcgtcaaagg gtccgtggag gtcgatgcct tcggcctcga
     3241 gaagatagac cctattctga acggcgtcgt caacgggaaa ggggagttcg tcttctacga
     3301 gggagccctt accctcaccg gtaaatgcga ttcaccgaag ctcacaatac gggacacctg
     3361 gcttaagaaa gccctcgtcg tcaacaaggt tacggcaagg tccacgatca cgacgaaagg
     3421 ccccgatgtt cacatagccg tgtacgacac gaaatacagg gatgccccct tcaccatcga
     3481 cgtgagcatg aaaaacctcc ttttctcacg tctcgatatc acgtcgggtt ccatacccat
     3541 gagcgccgtg agggagtatc tcatgatgga cgaagcaggg tatgacgtct gggcgtatgt
     3601 cagggatggg ttcctgaaga tcaggaagat aacctatgag aagaagaaac cttttatcgc
     3661 cgaactggaa ttgaagggtc tgacgggcga atacgaaggc aagaggctca cagatatttc
     3721 cggtgtgctt gagatgatag ataacaaggg gaccctgtcg gagggcaaag gtttcttcaa
     3781 agccacctcc ttccacgatc tcaaggggac catggagttc gggaagaagc cccgtataca
     3841 gctgacggga aagtacgtca ttgatctgca gcacgtcggc gagtttgtgg acatgagggg
     3901 tgtggtcgtc cagaaaggca ccgcggaggg caccgtcgag gtggacagcg gacagggaaa
     3961 gcacccgctg aagctcgggg gctcgggccg gataaaaggc gcggaaatct cctggaaggg
     4021 acaatctttt acggtggacg gtcccttcag gcttgccggc cgggaaatga ccttcgatcc
     4081 cattgtggtg accggccggg agacgcaggt gaccatgcag ggcaaatggg gaccccaggg
     4141 tctcacaacc tccctgaaag gctacatcga cccggggctt ccgggcaggt ttgcgggcaa
     4201 aaccgtccgg gcgtcgggga aggccgtcgt cgatgcccgt gtgacggtag ccgacgggca
     4261 gataggagtg aacgggaacg ccaatatgga tgatgtgatc ttcgccgtgc cgggcttcat
     4321 acgaaagacg aagggcgttc agagcagggc cgcggtgaaa ttcacgagga aaaagacggg
     4381 cgagatagtt gtggatgacc tctcgtgtaa cctcgacacg atcaacgtcc gtgcctcggg
     4441 aaagataagc ggcgacggcg ggatcgagag ccgggtcacc ctgcgcgcga aggatacggg
     4501 gagggcggca tcgctcttct ctctcggcga ggacataagg ggcggagacg tatccatcga
     4561 tcttgccgtc agcgaccttc gattccccct gacgaaactt ccctgggtcg tcggttccgc
     4621 gtcgatgaaa aaaggcttca tgaaggtgcc cggcattccg aaggtccttg ccaacatcga
     4681 cctcagggca ggtttccgcg gccacgagtt cga
//
LOCUS       DAOPOA010000087         4681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_19937_length_4681_cov_2.812797, whole genome shotgun sequence.
ACCESSION   DAOPOA010000087 DAOPOA010000000
VERSION     DAOPOA010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19937_length_4681_cov_2.812797"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKJ17_02865"
     CDS             <1..411
                     /locus_tag="PKJ17_02865"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOD74940.1"
                     /translation="HSPAPLFVAGPLPIDTAVGYDHIAGCVGASIASGAGADYLCYLT
                     PAEHLSLPTPDQVRAGLIAFKIAAHIGDSIKYGMDDEDLALARSRSRLDWDGQVAHAI
                     DPETARALAPEPGPCTMCGDFCAVALMKRLREQR"
     gene            612..860
                     /locus_tag="PKJ17_02870"
     CDS             612..860
                     /locus_tag="PKJ17_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74941.1"
                     /translation="MMMDISRLKYRVEVLGDHDDIKRYGPFNEQKAREFFEVEESFGG
                     TARIVRLEGDIQQKWKVLVECGDWDNYERGHREKEVIL"
     gene            complement(1261..1665)
                     /locus_tag="PKJ17_02875"
     CDS             complement(1261..1665)
                     /locus_tag="PKJ17_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74942.1"
                     /translation="MQKGSGKPEYLLKRFNPAEKPFYRTVLLVEFRIEPERSSAFRMF
                     PGSPVYRNITFDSSFPVVLTNFQGIVGCICGDDPVMIRHFGDLKCFESWLVEPGIMDI
                     CRGNYADKREPVPIDQSTQLVPVYLFIAIIAD"
     gene            complement(1646..1882)
                     /locus_tag="PKJ17_02880"
     CDS             complement(1646..1882)
                     /locus_tag="PKJ17_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74943.1"
                     /translation="MTDRKKNRNQGVIRLREKGKVAVRGKVPAGVMTAQQMAAISRIA
                     KEFGDGTIGLTARLNVELPGVDRRNAGEVAERLR"
     gene            complement(1938..2465)
                     /locus_tag="PKJ17_02885"
     CDS             complement(1938..2465)
                     /locus_tag="PKJ17_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOD74944.1"
                     /translation="MNTASSCNGSGGKQAAGSMDQSVFLDACVFYDCLEHPRCKQILD
                     RAHNLGFKICTSITVLGETLITMLRRDDADDHIAAFVSLLKEWDMLFVVPSDGVRIIC
                     YELGEDYTDTRMLGQATDRTHLAYAMAYGFKHFISTDPVIRTYSLPRKVLDMGFEKPE
                     VYNLIEFRNYVLNRR"
     gene            2835..3485
                     /locus_tag="PKJ17_02890"
     CDS             2835..3485
                     /locus_tag="PKJ17_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74945.1"
                     /translation="MASFCRQSYVLLWAGLLKGLQLALWPRLRPALGDYAYPAAYPVS
                     LLLFALATWYCGILRIPVALALLVFAALGTWGLRRGDYLIEGGHDLGDVIVGGRVYNG
                     TALPELAGRYVFGYWSDGFVPGDAILLVATPPPGWNATAFPDFAANLTPEDIGMWSVQ
                     RLNVTGPPAGEAFLLGFGEDEESELYALTTGVAGPDASTATGRVWKIVPAEAASGI"
     gene            3592..3909
                     /locus_tag="PKJ17_02895"
     CDS             3592..3909
                     /locus_tag="PKJ17_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOD74946.1"
                     /translation="MTLEAARAAERVVVAIAAENFGFNTETITVPTGAEVMIEFDNRD
                     DGVPHNVAIYTDSSAADAIFVGEIVTGPTRATYTLTAPETLGNYFFRCDVHPPMHGAF
                     IVT"
     gene            complement(4071..>4681)
                     /locus_tag="PKJ17_02900"
     CDS             complement(4071..>4681)
                     /locus_tag="PKJ17_02900"
                     /inference="COORDINATES: protein motif:HMM:NF009511.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOD74947.1"
                     /translation="PRTAFAVFLVLAGLAAALAATLPAPHNLYVLGIMGYSYMVEYFY
                     QTKKRDQKTPVAQLVGRTDFALFPVAGYLVHGSPDATALLFFLFFYPWTLAHLGANDI
                     ADIVNDRARGMATIPVLYDMKGATAWVLGFSVVHAGMALIFGLALGPIALAGFAVGIV
                     LLGMANYLVLRGKSADAAMRALPLMHATVIVYAVAIIASAAL"
BASE COUNT          935 a         1422 c         1373 g          951 t
ORIGIN      
        1 cactcccccg cgccgctgtt tgtcgcaggc cccctcccca ttgataccgc tgttgggtat
       61 gaccacatcg ccgggtgcgt cggtgcaagc atcgcgagcg gcgcaggcgc cgattacctc
      121 tgctacctca ccccggccga gcacctcagc ctcccgaccc ccgaccaggt cagggcgggg
      181 ctcatcgcct tcaagatcgc cgcccatatc ggcgactcga tcaagtacgg catggacgac
      241 gaggatctcg ccctcgcccg ttcccgttct cgcctcgact gggatgggca ggtcgcccat
      301 gccatcgatc ctgagactgc acgggctctc gcccccgaac cgggaccgtg cacgatgtgc
      361 ggggacttct gtgcggtcgc cctgatgaag cggctcaggg agcagaggtg aggattgtca
      421 ggctttttct gaccttcatt ccctgatccc ataactctgg catccctccg gtgccgcgtg
      481 cctggtgcca tgacctcatc aaccccggac ctgagcgcgg cggttcccct cccagggccc
      541 tctcacccac ctcgtatcgg tgggagatcc ccaaggttta tagatctgca ggtgattctc
      601 tggaccggtg aatgatgatg gacatctccc gtctgaagta cagggtagag gttctcggcg
      661 accatgacga catcaagcgc tatggtccgt tcaacgagca gaaggcacga gaattcttcg
      721 aggtcgagga gagtttcggc gggactgccc ggatcgtgcg gctggagggg gatatacagc
      781 agaagtggaa ggttctcgtc gagtgcgggg actgggacaa ctacgagaga gggcatcgtg
      841 agaaggaggt gatcctataa ttataagtcc cctcagataa aaccgggggt aatggtattc
      901 cgggaaattg gcgatgatct ctgggagatt gttcagaaat acctcccccc caacaaagcc
      961 acacatcggc cggccccggt gtgatccccg cggactgatc aacggtattt tgtacgttct
     1021 cactaccgga tgctcctggc tcgatgtacc ggcgaactac ggtaccaaat cgacggaaag
     1081 gagtgtacca ggcaatcttt ctcgacctgc tccagtcggg gtacgagatc aagaagatcg
     1141 atctctcgca ttgtgcgacc gacaccaagg atatcccggc aaaaaactac atgatgccca
     1201 caaggccaca tgcataccag atgaaaatat ctagagggcc attttcatac gaaaaaggga
     1261 tcaatcggct atgatggcta taaaaaggta aacgggaaca agctgagtgc tctggtcgat
     1321 cggaacgggc tcccgcttgt ctgcatagtt tcccctgcaa atgtccatga ttcccggctc
     1381 tacgagccaa ctctcgaagc atttgagatc cccaaagtgc cggatcatca cggggtcatc
     1441 tccgcagatg caacctacga tgccctggaa attcgtcagt acaaccggaa acgaggaatc
     1501 aaaagtaata ttccggtaaa ccggagatcc cggaaacatc cgaaacgcgg aagaccgttc
     1561 tggttcgatc cggaactcta caagaagcac agtgcggtag aacggttttt cagctggatt
     1621 gaagcgttta agaagatact ccggcttacc ggagcctttc tgcaacctcc cccgcgttcc
     1681 gccggtcgac gccggggagt tccacgttga gccgggccgt cagcccgatc gtgccgtccc
     1741 cgaactcctt tgcgatccgg gatattgccg ccatctgctg cgcggtcatc acgccggcgg
     1801 gaacctttcc ccggacggcg acctttccct tctcccggag gcggatgacc ccctggtttc
     1861 tgttcttctt cctgtcggtc atgttgtacc tgccttagga tatgggaaga attggtgtta
     1921 aatcataccc gctgccttta tcggcggttc aggacatagt ttctgaattc gatcaggttg
     1981 tagacttcag gtttttcaaa gcccatgtcg aggacctttc ttgggaggct gtatgtcctg
     2041 atgactggat ctgtagaaat aaagtgctta aacccgtatg ccatcgcata tgccagatgg
     2101 gtcctgtcgg tggcctgccc aagcattctt gtatcggtgt agtcctcccc aagttcataa
     2161 cagatgatcc tgacgccgtc actcgggaca acaaagagca tgtcccattc tttcagcagg
     2221 gaaacaaacg ctgcaatatg gtcgtcggca tcgtcccgtc tgagcattgt gatgagtgtc
     2281 tcgcccagaa ccgttatcga ggtgcagatc ttgaatccga ggttgtgagc ccggtcaagg
     2341 atctgtttac atcgtggatg ctcaagacaa tcataaaaga cgcaggcatc cagaaagacc
     2401 gattggtcca ttgaacctgc tgcttgttta ccgcctgagc cgttgcatga ggaggcggtg
     2461 ttcatcctcg cttatggcgt atgcaatctg cccgcggcaa tcaaccgcac caccatacgg
     2521 gagcataccc cgctcgtatt tttggccaac gttcactctc ggatcgcctt tctctgtcat
     2581 ttcaccacgc gcctggaagg tggtgcacaa tcatcttctt cctggtgaga gatgacaccg
     2641 taccccttaa gcgacacgcc cttcctatgt gaggtgtatc gatcaggtac attaaaaaac
     2701 gatctgtttt gtccgacctg ttagcctctc caggttcccc ggcgcaccag tctgtgggag
     2761 catctcccgg ttccattctc tcctcgccgg ccgagaacac aaaccgcata taccctcccc
     2821 cctgatacca gggtgtggcc tcattttgca ggcaatccta cgtcctgctc tgggccgggc
     2881 tcctgaaagg cctccagctc gccctctggc cccgtcttcg ccccgcgctc ggggattacg
     2941 cctaccccgc ggcatacccg gtctccctcc tcctcttcgc cctcgcgacc tggtactgcg
     3001 gcatcctccg tatcccggtc gctctcgctc tcctggtctt cgccgctctc gggacctggg
     3061 gcctgcggcg gggcgactac ctcatcgagg gcggccatga cctgggcgac gtcatcgtcg
     3121 gcggccgggt ctacaacggc acggcgctcc cggagcttgc gggacgctac gtcttcggct
     3181 actggagcga cggtttcgtc cccggtgacg ccattttgct cgtcgcgacg ccccctcccg
     3241 ggtggaacgc aacggccttt ccggacttcg cggcgaatct gacacctgag gacatcggga
     3301 tgtggagcgt gcagaggctc aacgtgaccg ggccacccgc gggagaggcc ttcctcctcg
     3361 gattcggtga ggatgaagag tcggagctct acgcgctcac gaccggcgtc gccggcccgg
     3421 atgcgtccac cgcgaccggc agggtctgga agatcgtccc ggcggaggca gcttccggga
     3481 tatagagggt gccggtaccg gccggggcaa cctttatggc tcccgctcac ctggagggcg
     3541 acacatggca cgaatagcaa caggcagggc atccgtcacg ggggtgcggg cgtgaccctg
     3601 gaagcggccc gcgccgccga gagggtggtc gtcgcgatcg cggccgagaa cttcggcttc
     3661 aatacggaga caataacggt ccccacaggc gcggaggtga tgatcgagtt cgacaaccgg
     3721 gatgacgggg ttccgcataa cgtcgcgatc tacacggact cttccgcggc cgatgcgatc
     3781 ttcgtcggcg agatcgtcac cggccccacc cgcgccacct acaccctcac ggcgcccgag
     3841 accctgggaa actacttctt ccggtgtgac gtccatcccc cgatgcatgg ggcgttcatc
     3901 gtgacgtagg agggcccacc ggtatacttc aggtttgcaa acatctcgtc gctgaacgag
     3961 gaaagtatcc ggttctcccg gcggaacggg tatgttgagt acgggcactt caggaacgtc
     4021 ggcacaaagc gcggggttct cttcgacgtc gtccagatgc gaaaggagat ctagagagcg
     4081 gcgctcgcga tgatcgccac ggcgtagacg atcaccgttg catgcatgag cgggagggcc
     4141 cgcatcgctg cgtccgcgct cttcccccgg aggacgaggt agtttgccat accaaggagc
     4201 acgatcccca ccgcgaaccc tgcgagtgcg atcggcccga gcgcgaggcc gaagatgagc
     4261 gccatacccg cgtgaacgac cgagaagccg agaacccagg cggtcgcgcc cttcatgtcg
     4321 tagaggaccg ggatcgtcgc catcccccgg gcgcggtcgt ttacgatatc ggcgatgtcg
     4381 tttgccccga ggtgcgcgag cgtccaggga tagaagaaga ggaagaagag gagagcggtc
     4441 gcgtccgggc tgccgtgcac caggtagccg gcgacgggga agagcgcgaa gtccgtccgc
     4501 ccgacgagct gggcgacggg ggtcttttga tcccgcttct ttgtctggta gaagtactcg
     4561 accatgtatg aataacccat gatcccgagg acgtagaggt tgtgcggcgc gggcagggtt
     4621 gcggcaagcg cggccgcgag gcctgcaagg acgaggaaga cggcgaatgc cgtccggggg
     4681 g
//
LOCUS       DAOPOA010000088         4679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_19947_length_4679_cov_3.254758, whole genome shotgun sequence.
ACCESSION   DAOPOA010000088 DAOPOA010000000
VERSION     DAOPOA010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19947_length_4679_cov_3.254758"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKJ17_02905"
     CDS             complement(<1..453)
                     /locus_tag="PKJ17_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74948.1"
                     /translation="MEKVIVLTARELGYQVVKALAAEGIGSIVIYGHEPDEIAQFSKH
                     VIETHEIPGYFDRQGVLTDFLLDRKDEWKGTLIIPTDDYGVKFLAANRDRLSAHYVIP
                     TPDLDVVNTVVDKKSLYAVARHIGIGVPAIHSPGSMEELRSLEGILTFP"
     gene            complement(441..1526)
                     /locus_tag="PKJ17_02910"
     CDS             complement(441..1526)
                     /locus_tag="PKJ17_02910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOD74949.1"
                     /translation="MIDCIFTGDIMPGARRVDEVPDPGLLGHFKEADLVVGNLECPIV
                     SEPPAVMDTRKIPLWSDAANLPILTSFGFTHVSMNNNHIFDLFEKGLDETQGLLDNAG
                     IGAFGIDYGVLSQYRRVSVKGITLGMAAVNWVETRFCGHLSRDLRDLDVGSLKKDCDF
                     LIMFLHWGDDHNIFINADQQEAGRRLIDQGADLVIGHHPHVTQGSEVYKGKHIFYSLG
                     NFIFTPREEYESLPYSIRYEDCRENVLFQRPECKIGMYVRVVFDRTGYEVAGVCPVYR
                     EETLPAVLPASLASFFDGMLEQMNSQVAGSAYELNEAGRRMILTRYTLPLILTHPLYW
                     PVFFRKLNVGKAVRFVRQGRVGKLWKK"
     gene            complement(1530..2207)
                     /locus_tag="PKJ17_02915"
     CDS             complement(1530..2207)
                     /locus_tag="PKJ17_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD74950.1"
                     /translation="MFSQKTMMLYTQSIKDVASMDIRCAEGLLCRELLPRDRGTFAKL
                     LGTQRKRETLFQPVFGMEDVEERVARGEHCFVCEDGERIAGYIWFSPREKFILEIKVT
                     LQLQDGDVYAYNAYVDEEYRGRNITPSILVAACRVLLPAGFTRLLLVTMDWNESTHRT
                     LKKAKFLCKGNLTAGYFATIRFMINTCGDIDTLNNAGPFELYGKLFRKVAAALSGKGA
                     TARPRMV"
     gene            complement(2290..3063)
                     /locus_tag="PKJ17_02920"
     CDS             complement(2290..3063)
                     /locus_tag="PKJ17_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74951.1"
                     /translation="MSTLRRIGRYIFSRTTMVFYEYHYNNPSPWSCPRNGFVFRPLAM
                     PSDHDSLSEILAIQEGTEHVFRPIDVEEAARRLEEGEACYIGEDAGRIVGYSWFAKGE
                     KYIPEIESTIRLRPSDLYLYNSYILKDYRRRNIVGGNVDAARKDLVPRGFTREITATM
                     KWNKAAGGSLLKAKFQVAGTVTAGYFLTFRYMINGCGNIAFRNETGMFGLYRKIFRRL
                     GFPSVRQVSSCPDRPDDGLPGRGAGMSEKPLAPRRELLT"
     gene            complement(3103..4185)
                     /locus_tag="PKJ17_02925"
     CDS             complement(3103..4185)
                     /locus_tag="PKJ17_02925"
                     /inference="COORDINATES: protein motif:HMM:NF025755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HOD74952.1"
                     /translation="MIDLLRSIHHYSSHFAQFPRLLQGIAQPHPGCAPPGKSRAAIAL
                     DHLYLFFVLKVVPVNYYLFEFQFKDRKHFREYMDETVAPLLKHRFYKCLWNDSYSSLV
                     NDKHLFHCLCRYHGISVPELYGMCARLPGDNDPCLVSVMDARGLDRVILKPRGGVQGR
                     GIYFARRNGRGIELKAAATPEVAPGGDFIVQEVIGQHPRMEGMNPFCLNTIRLITLLT
                     RDGEVEFLAAMLRTSSDGTAVDNFSLGGIVVGVDIETGKLKERGFQKTTHAVELRRHP
                     LTGVVFKGFEVPYWNEVKETAAKAQKVFSELKTIGWDLAVSPSGPVMIEGNIEWGTTG
                     IQATNGGLLTQKNRALFARYGLTFHT"
     gene            complement(4182..>4679)
                     /locus_tag="PKJ17_02930"
     CDS             complement(4182..>4679)
                     /locus_tag="PKJ17_02930"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD74953.1"
                     /translation="NFGDGTTSTEQNPAHNFLTAGIYPVTLTVTSASGSSAKTKSDYV
                     QSFSIDAIIVADNPYHYKPHFSGFGGFYDKTILYGNTGVTSSELKYARLFYGSCNSCN
                     YYAGIYHRGILICTTSDTDLYTALNYFQDYLSGMSDVEILTHLNTIQDTHEMLNFNLK
                     PPSLR"
BASE COUNT          919 a         1391 c         1358 g         1011 t
ORIGIN      
        1 ggggaacgtg agaatgccct ccagcgagcg gagctcctcc atcgaccccg gcgaatggat
       61 ggccggcacc ccgatgccga tgtgccgggc gacggcgtag agcgactttt tatccacgac
      121 ggtgttcacg acatcgaggt cgggggtggg gatgacgtag tgggccgaca acctgtccct
      181 gttggcggcg aggaatttga ccccgtagtc gtcggtgggg attatgagtg ttcccttcca
      241 ttcatccttc ctgtcgagga ggaagtcggt caggacgccc tgcctgtcaa agtagccggg
      301 tatctcgtgg gtctctatga cgtgcttcga gaactgggcg atctcgtcgg gctcgtgccc
      361 gtagatcacg atgctgccga taccctccgc ggcgagggcc tttacgacct ggtacccaag
      421 ttcccgcgct gtcagaacga tcactttttc catagctttc ccaccctccc ttgccttacg
      481 aagcgcacgg ccttccccac attgagcttt ctgaagaaaa cgggccagta aaggggatgc
      541 gtcaggataa ggggcagggt gtaccgggtg aggatcatcc tcctgcccgc ctcgttgagc
      601 tcatacgccg atcccgccac ctggctgttc atctgttcca gcataccgtc aaagaaggat
      661 gcgagagagg caggcagcac agccggaagt gtctcctccc ggtacacggg acacaccccg
      721 gcgacctcat aacccgtcct gtcgaagacc accctcacgt acatgccgat cttgcactcg
      781 ggacgctgga agaggacatt ctccctgcag tcctcgtagc gtatgctgta gggcaggctc
      841 tcgtactcct cccggggcgt gaagataaag ttccccaggg agtagaatat gtgcttcccc
      901 ttgtagacct cgctgccctg cgtcacgtgg gggtggtggc cgatgacgag gtcggcgccc
      961 tggtcgatga gccgtctgcc cgcctcctgc tggtcggcgt tgatgaagat gttgtggtcg
     1021 tcgccccagt ggaggaacat gatgagaaaa tcgcagtctt tcttcaggga ccccacgtcg
     1081 agatcacgga gatccctgga aaggtggccg cagaaccgcg tctccaccca gttgaccgcc
     1141 gccatcccca gggtgatccc cttgacgctc acgcggcgat actgggacag caccccgtag
     1201 tcgatgccga aggcgccgat acccgcgttg tcgagaagac cctgcgtttc atcgaggcct
     1261 ttttcgaaga ggtcgaagat gtggttgttg ttcatgctca catgggtaaa accaaagctc
     1321 gtgagtatcg gcaggttggc cgcgtccgac cacaggggta tcttcctcgt gtccatgaca
     1381 gcgggcggct ccgagacgat gggacattcc aggttgccga cgaccaggtc ggcctctttg
     1441 aagtgcccga ggagccccgg atcgggcact tcgtccaccc gtcttgcccc gggcattatg
     1501 tcgcctgtga agatgcagtc gatcattgat cacaccatcc tcgggcgcgc ggtcgcgccc
     1561 tttccggaaa gggccgccgc aaccttgcgg aaaagctttc cgtaaagctc gaaggggccc
     1621 gcgttgttca acgtgtcgat atcgccgcag gtattgatca tgaaccggat cgtggcaaaa
     1681 tatccagcgg tgagatttcc tttgcacagg aacttcgctt tcttcagggt cctgtgcgtg
     1741 ctttcattcc agtccatggt gacaagcaga agcctcgtga agcctgccgg cagaagtacc
     1801 cggcaggccg cgaccaggat cgacggggtg atgttcctgc ccctgtactc ctcgtcgacg
     1861 taggcgttgt aggcatacac atcgccgtcc tgcagttgca gtgtgacctt gatctcgagg
     1921 atgaactttt cgcggggact gaaccatata taccccgcga tccgttcccc gtcctcacag
     1981 acaaagcagt gctcccccct cgctacgcgc tcttcgacat cctccatacc gaatacgggc
     2041 tgaaaaaggg tttccctctt tctctgggtc ccgagcagct tcgcgaaggt cccgcggtcc
     2101 cggggcaaca gctctctgca caggagacct tccgcgcacc tgatatccat ggaggctacg
     2161 tctttgatcg attgcgtgta gagcatcatc gtcttttgtg agaacatata ccgtctcacc
     2221 ttaggcagaa aaccggccat gaaccctctt ttcggtttca cccctattca tggaatgtga
     2281 gaccgtcgct cacgtgagca actcccgtcg aggcgcaagg ggcttttccg acatgccggc
     2341 gccacgaccc ggtagaccat cgtcgggtcg atccgggcat gatgaaacct ggcgaaccga
     2401 cgggaacccg agcctgcgga agatctttcg atagaggccg aacatgccgg tctcgttccg
     2461 gaaggcaata ttcccgcaac cattgatcat gtacctgaag gtcaggaaat aacccgccgt
     2521 cacggtgccg gcgacctgga acttcgcttt caaaagggac cctcccgcgg ccttgttcca
     2581 cttcatggtg gcggtgatct cccgggtgaa accccggggg acgaggtctt tcctggccgc
     2641 gtcgacattg ccgccgacta tgttgcggcg gcggtaatcc ttcaatatgt atgaattgta
     2701 gagataaaga tccgacggcc tgaggcggat ggtcgattcg atctcgggaa tgtacttctc
     2761 ccccttcgca aaccaggaat acccgacgat cctgcccgcg tcttccccga tatagcaggc
     2821 ttcaccctcc tccagtcgcc tcgcggcttc ctccacgtca atgggcctga aaacgtgctc
     2881 tgtgccctct tggatggcga gtatctccga caggctgtca tggtcggagg gcatcgcgag
     2941 gggcctgaaa acgaacccgt tccgcgggca actccagggg ctcgggttat tgtagtgata
     3001 ctcgtaaaaa accatcgtgg tcctggaaaa gatataccgc ccgatcctgc gcagggtact
     3061 caccgctcct gtcgcgttat gaattgacat ggttgcacct ctttaagtgt gaaacgttag
     3121 cccgtaccgg gcaaaaagag ccctgttctt ctgcgtcagg agtcccccgt tcgtggcctg
     3181 gatgcccgtg gttccccatt cgatattccc ctcgatcatc accgggccgg aaggcgagac
     3241 cgccaggtcc caaccgatcg tcttgagctc gctgaaaacc ttttgcgcct ttgccgccgt
     3301 ttccttcacc tcgttccagt aggggacctc aaaacccttg aaaacgacac ctgtgagggg
     3361 atgtcttctc aactccaccg cgtgcgtggt cttctgaaag ccacgctctt tgagtttccc
     3421 cgtttcgatg tccaccccga ccacaatccc ccccagtgag aaattatcga ccgccgtccc
     3481 gtctgaactc gtccgcagca tcgcggccag aaactccacc tcaccgtcgc gggtgagaag
     3541 cgtgatgagg cgtatggtgt tcaggcaaaa agggttcatg ccctccatgc gcgggtgctg
     3601 cccgatcact tcctgaacga tgaaatcccc ccccggagcg acttccggcg tcgccgcggc
     3661 cttcaactcg atcccccgcc cgttgcgccg cgcgaaatag atccccctcc cctgtacccc
     3721 gccccggggt ttcaggatca ccctgtcgag accccgcgcg tccatcacac tgacgaggca
     3781 ggggtcgttg tcgcccggca accgggcaca cataccgtag agctccggga cagatatgcc
     3841 atgataccgg cacaggcaat ggaaaaggtg cttgtcgttc acgagggaac tgtagctgtc
     3901 gttccacagg catttgtaga acctgtgctt cagaagcggc gccacggtct cgtccatgta
     3961 ttccctgaaa tgcttccggt ctttgaactg gaactcgaaa aggtaatagt tgacgggcac
     4021 gaccttgagc acgaagaaga ggtacaggtg gtcgagggcg atggcggccc gggatttccc
     4081 cgggggggcg cagccgggat ggggctgcgc gatcccctgg agcagtctcg ggaactgtgc
     4141 aaaatgggac gaatagtgat ggatggatct cagcaggtct atcatcttag cgacgggggc
     4201 ttgaggttga agttgagcat ctcgtgggtg tcctgaatag tgttgaggtg ggtcaagatc
     4261 tcgacgtcgc tcatcccgct caggtaatcc tggaagtaat tgagggccgt atagagatcc
     4321 gtatcgctcg tggtgcagat caggatgccg cggtggtaga tccccgcgta gtagttacag
     4381 gagttgcagg aaccatagaa gaggcgggcg tacttgagct cgctcgaggt caccccggtg
     4441 ttgccgtaca ggatggtctt gtcatagaag ccgccgaagc ctgagaaatg aggcttgtag
     4501 tggtacgggt tgtccgccac gatgatggcg tctatggaga aactctggac atagtccgac
     4561 ttcgtctttg cgctgcttcc gctggcagat gtgaccgtga gggtcaccgg gtatattccg
     4621 gcggtcaaga agttatgggc aggattctgt tccgtgctgg tcgtgccgtc gccgaaatt
//
LOCUS       DAOPOA010000089         4676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_19969_length_4676_cov_3.058645, whole genome shotgun sequence.
ACCESSION   DAOPOA010000089 DAOPOA010000000
VERSION     DAOPOA010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19969_length_4676_cov_3.058645"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..853
                     /locus_tag="PKJ17_02935"
     CDS             191..853
                     /locus_tag="PKJ17_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOD74954.1"
                     /translation="MRSIVCLMAVLCLVLVSTAAVHGQGAAPGAGIRLPEPKYDGNVS
                     VEKALKERRSVRVYKDSPLSVAEVSQVLFAAQGITEPGRGLRTAPSARARYYLTVYLF
                     AGNVTGLKPGMYRYVPQGHSLALVAGGDAKGMLYKAAGQAPVDKAPAALVIAGSQARA
                     TNEGWMYLEAGHAAQNVYLQCVPLGLGTVVMAGFNADAVDKVIGLPAGERTLYIMPLG
                     RK"
     gene            916..1380
                     /gene="sixA"
                     /locus_tag="PKJ17_02940"
     CDS             916..1380
                     /gene="sixA"
                     /locus_tag="PKJ17_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HOD74955.1"
                     /translation="MLLYLVRHGEAKREDEDPERGLTDNGMRHARRMGEFLAFMDVSL
                     DAVIHSTKKRAAQTAQILTESIRPASGIREENGLSPNDDPAPWVERISAMTADTAIVG
                     HLPFLDRLLGLLVLGDGGRTIIDFRPAGTVCLKPSGDGRWLIAWVLSPEVIW"
     gene            1377..2741
                     /gene="rmuC"
                     /locus_tag="PKJ17_02945"
     CDS             1377..2741
                     /gene="rmuC"
                     /locus_tag="PKJ17_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOD74956.1"
                     /translation="MSYLSLVIAFLAGVALGGVVFSLVVASRARARQAELEARAGSAE
                     AVVTELRQQFQQGRDEALRLQAELDGEREARVQAMTRCETLDLRLKEEMERFEAVRKE
                     LSETFKALSLDALASNTNEFRKYADEFMKLAEEKLKSQTAEGKKELEGKKELIDRNID
                     SIGKTLTEVQRRVEEVGKVSGERITEVTTLIKKHEEVTAKLRDTTEHLGRALASSKKR
                     GEWGERMAEDIIRLVGMAEGINYVKQTTLENTAGRPDFTFFLPNDLKVNMDVKFPLDN
                     FAHYLDAESDHDRKRYRDELLKNAKVMIKQVTTREYINPAENTVDYVIVFIPNEQVYS
                     FIQESDASIMDEALRQKVILCSPFTLYAVLAVVRQTIENVKLERTASEILRLLAEFSK
                     QWKLYKDKFKTMGDRIDAARKEYEVLVTTRTNMLERPLRKIDELSAADALELGTGELS
                     QDES"
     gene            2731..3183
                     /locus_tag="PKJ17_02950"
     CDS             2731..3183
                     /locus_tag="PKJ17_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74957.1"
                     /translation="MKASLSSLSRLVLVLLLSAPCLVAGADGGKDLPGFLEGNYGLVG
                     RRPGTGETYSGTVTIKARDGGLEVIRCVGGSRHAGQGSIVLVTADRIPALSVRWGDGK
                     KEYRGRYDIHGDADNYARLSGPYVAMEDEREFGWELLYVDPGNGPVCR"
     gene            3225..3428
                     /locus_tag="PKJ17_02955"
     CDS             3225..3428
                     /locus_tag="PKJ17_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74958.1"
                     /translation="MEDEDRLRTVLKQLIQNNEYLAENYRKWGRVAATGGLDAVSKEI
                     DEAGQFIQIATQKLRGALGLMGE"
     gene            complement(3415..4185)
                     /locus_tag="PKJ17_02960"
     CDS             complement(3415..4185)
                     /locus_tag="PKJ17_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOD74959.1"
                     /translation="MGIITLLTDFGVKDPYVGVMKGIILGIDPGCRIVDMTHEVEPQD
                     MREAAFLIDDYWRHFPPGTVHVAVVDPTVGSARRPLIVSAKGHHFVGPDNGVFTLLVD
                     NAPDIHVIENRNFMLSPVSSTFHGRDVFSPAAARLSRGYSPQAFGRKITDPVLLSDIH
                     PVIEGRTLKGSVVRFDRFGNAITNIKGHALRYFAGEGPFEIRMGKHVFAAIGKTYCEG
                     GLACLEGSSGYLEFGVFKGDFRRETGTGKGDPVTVTLP"
     gene            complement(4198..>4676)
                     /locus_tag="PKJ17_02965"
     CDS             complement(4198..>4676)
                     /locus_tag="PKJ17_02965"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOD74960.1"
                     /translation="VIATETRPYMQGSRLTVWELMQEGIPVELVPENHAGLLCSRGAV
                     NKIITGADRIAANGDTANKIGTFMIALAADAYKVPFYIAAPVSTFDKAVEDGGRIEIE
                     ERSAREVKYLDDRLLTVRDAKARYFGFDVTPSRYISGFITEKGIIKKPFAKYIRML"
BASE COUNT          932 a         1362 c         1551 g          831 t
ORIGIN      
        1 accctcgacc tcttgactgc cagtcaagca ctctcccaac tgagctacag ccccgcaggt
       61 aagtgttata acagcatgag gatgaattgt caatggctat tgcgccgccc aaggaggtgc
      121 cttaagagtt gcaaaggtgc ggaaaactta ttaccatgtg agggtaacga taccgttcag
      181 gaggtgctcc atgaggtcca tcgtttgttt gatggcggtc ctttgtcttg ttctcgtgtc
      241 gacagcggcg gtgcacgggc agggcgcggc acccggtgcc gggatcaggc tgcccgagcc
      301 gaaatacgac gggaacgttt ccgtcgagaa ggcgctcaag gagaggcgat cggtgagggt
      361 ctacaaggat tctcccctgt cggtggccga ggtctcccag gtgctcttcg cggcccaggg
      421 tataacggag ccgggaaggg gcctgaggac ggcgccttcc gcgagggccc ggtactacct
      481 caccgtctat ctctttgcag gcaacgtgac gggacttaag ccgggcatgt accgctatgt
      541 gcctcagggg cattcgcttg ccctcgttgc cggcggggac gccaagggga tgctttacaa
      601 ggccgcgggc caggcgccgg tagacaaggc gccggcggcg ctcgtcatcg ccgggtcgca
      661 ggcaagggcg acgaatgagg gctggatgta tctcgaggcg ggccatgccg cgcagaacgt
      721 ctatctccag tgcgtgccgc tggggctggg tacggtcgtc atggcggggt tcaacgccga
      781 cgcggtggac aaggtcatcg gtcttcccgc cggcgaacgg accctttata taatgcctct
      841 cggcaggaaa tagattatac tgtattcccg gcggcgcggc ctgtcggaga caccacagaa
      901 caggaaagga caacgatgct gctctacctt gtgcgccacg gcgaagcgaa gcgcgaggac
      961 gaggaccccg agcggggcct cacggacaac gggatgaggc acgcccggag gatgggcgag
     1021 ttccttgcct tcatggacgt cagcctcgac gctgtcatcc acagcacgaa gaagagggct
     1081 gcccagacgg cgcagatcct cacggaatcg atacggcccg cttccggcat ccgggaggag
     1141 aatggcttgt ctcccaatga cgacccggcg ccatgggtag aaaggataag cgccatgacc
     1201 gccgacactg ccatcgtcgg tcatctccct ttcctcgaca ggctcctcgg ccttctcgtc
     1261 ctcggcgacg gggggaggac catcatagac ttcaggccgg cgggaacggt gtgcctcaag
     1321 ccgtcgggtg acggaaggtg gctcatcgcc tgggtgctta gccccgaggt gatctggtga
     1381 gttatctctc ccttgtcatc gccttcctcg cgggagtggc cctgggaggc gtcgtcttct
     1441 cccttgtcgt cgcctcccgc gcgcgcgccc ggcaggcgga gctcgaggcg agggccgggt
     1501 ccgcggaggc ggtcgtcacc gagttgaggc agcagttcca gcagggcagg gacgaagcac
     1561 tgcgcctcca ggcggagctt gacggagaga gagaggccag ggtgcaggcc atgacgaggt
     1621 gcgagaccct ggacctgcgc ctcaaggagg agatggagcg tttcgaggcc gtaaggaagg
     1681 aactctccga aaccttcaag gccctttccc tcgatgccct cgcgtccaat acgaacgagt
     1741 tcaggaaata cgccgacgag ttcatgaagc tcgccgagga gaagctcaaa tcccagacgg
     1801 cggaagggaa aaaggaactg gaaggcaaga aggagctcat agacaggaac atcgactcca
     1861 tcgggaagac ccttacggag gtccagaggc gcgtcgaaga ggtgggcaag gtgagcggcg
     1921 agcgcatcac cgaagtgacc accctgatca agaagcacga agaggtcacg gcgaagctca
     1981 gggacacgac ggagcacctc ggccgagcgt tggcaagctc gaagaaaagg ggcgaatggg
     2041 gcgagcggat ggccgaggat atcatccgtc tcgtggggat ggcggaaggt atcaactacg
     2101 tcaagcagac gacattggag aacacggcgg gcaggcccga tttcaccttc tttctcccca
     2161 atgacctcaa ggtcaacatg gatgtgaagt tccccctcga caacttcgcc cactatctcg
     2221 acgccgagag cgaccacgac cggaagcggt accgcgatga gctcctcaag aacgcgaagg
     2281 tgatgataaa gcaggtgacg acccgggagt acatcaaccc cgccgagaac accgtggact
     2341 acgtcatcgt cttcattccc aacgagcagg tctacagctt catccaggaa tcggacgcct
     2401 cgatcatgga cgaagcattg aggcagaagg tcatcctctg ctcgcccttc acgctctacg
     2461 cggtcctcgc cgtggtccgc cagaccatcg agaacgtgaa gctcgaacgg acggcgtcgg
     2521 agatactcag actccttgcc gagttctcga agcagtggaa actgtacaag gacaagttca
     2581 agaccatggg cgaccgcatc gacgcggcga ggaaggaata cgaggtcctc gtcacgacgc
     2641 ggacgaacat gctcgagagg ccgctgagga agatcgatga actgagcgcg gccgatgccc
     2701 tggaactggg gacgggggag ctctcgcagg atgaaagcta gtctgtcctc attaagccgc
     2761 ctcgtccttg tgcttctcct gtcggcgccg tgcctcgtcg ccggcgctga tggcggcaag
     2821 gaccttcccg gttttctcga agggaactac ggtcttgtcg gcaggcgtcc gggcacgggc
     2881 gagacctatt cggggacggt gacgataaag gcgagggatg gcggcctcga ggtcatacgg
     2941 tgcgtcggcg gatcgcgcca cgccggtcag ggttccatcg ttctcgtgac ggccgacagg
     3001 atacccgccc tcagcgtgcg ctggggcgac gggaagaagg aatacagggg ccgctacgac
     3061 atacacggag atgccgacaa ctacgccagg ctctcggggc cttatgtcgc catggaagat
     3121 gaacgggagt ttggctggga acttctctac gtcgatcccg ggaacggccc ggtctgcagg
     3181 taagcgaggc cctccgcgga gcggccggat taaggaggct tgtgatggaa gatgaggacc
     3241 gtttacgtac cgtgctgaaa cagctcatcc agaacaacga gtatctcgcc gagaattacc
     3301 ggaaatgggg cagggtcgcc gcgacgggcg gtctcgatgc cgtgtcgaag gagatcgacg
     3361 aggccggtca gttcatccag atagccacgc agaagctccg gggtgccctc ggccttatgg
     3421 gagagtgacc gtcaccgggt cgccctttcc cgtccccgtt tcgcgcctga agtccccctt
     3481 gaaaacaccg aattccaggt agccggagct gccctcgagg caggcgaggc caccctcgca
     3541 gtaggtcttg ccgatcgccg cgaagacgtg tttgcccatc cgtatctcga aagggccctc
     3601 cccggcgaag taccgcaggg cgtggccctt tatgttcgtg atggcgttgc cgaagcggtc
     3661 gaagcgcacc acgctccctt taagcgtcct gccctcgatc acgggatgga tgtcgctcaa
     3721 gagaacggga tccgttattt ttcgcccgaa cgcctggggg gagtagccgc gggacagcct
     3781 ggcggcggcg ggggagaaga cgtcccggcc gtggaaggtg gaactgacgg gggagagcat
     3841 gaagttgcgg ttctcgatga cgtggatatc gggggcgttg tccacgagga gggtgaagac
     3901 gccgttgtcg ggcccgacga aatggtgtcc cttcgccgac acgataagcg gccgcctcgc
     3961 gctccccacg gtggggtcga cgacggcgac atggaccgtc cccggcggga aatgcctcca
     4021 gtagtcgtcg atgaggaagg ccgcctcgcg catgtcctgg ggttccacct cgtgggtcat
     4081 gtcgacgatc cggcaccccg ggtcgatgcc gaggatgatc cccttcatga cgcccacgta
     4141 ggggtctttg acgccgaaat cggtgaggag ggtgatgatg cccatcggcc ctgccggtca
     4201 gagcatcctg atgtatttcg cgaaaggttt cttgatgatg cctttctcgg tgatgaagcc
     4261 gctgatgtac cgggacgggg tgacgtcgaa cccgaaataa cgagccttcg cgtcccttac
     4321 ggtgaggagc ctgtcgtcga ggtatttcac ctccctggcg cttcgctcct cgatctctat
     4381 gcggccgccg tcctcgacgg ccttatcgaa ggtggagacc ggggcggcga tgtagaaggg
     4441 gaccttgtac gcgtccgcgg cgagggctat catgaaggta ccgatcttgt tcgccgtgtc
     4501 cccgttcgcc gctatcctgt cggcgccggt gatgatcttg ttcacggccc cccgggagca
     4561 caggagcccg gcgtggttct cggggacgag ttcgacgggg atgccttcct gcatgagctc
     4621 ccacaccgtc aggcgcgacc cctgcatgta agggcgcgtt tccgtggcga tgacct
//
LOCUS       DAOPOA010000090         4656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_20056_length_4656_cov_3.084764, whole genome shotgun sequence.
ACCESSION   DAOPOA010000090 DAOPOA010000000
VERSION     DAOPOA010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20056_length_4656_cov_3.084764"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PKJ17_02970"
     CDS             <1..1245
                     /locus_tag="PKJ17_02970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD74961.1"
                     /translation="EQGHGKDGGPAMIGSYGGISPRGDLLLLRKLADHLAGKTFLHIN
                     STREGGGVAEILQRMIPIMSSLGVNARWDVITGDQRFFDMTKKVHNALQGYTETIDDR
                     MWEHHFEINEANASHMNLDADAVLIHDPQPLPLVTFRKEGVWIWRCHIDVSNPQRDVF
                     EKIGKYATKFDAAIYSVARFAGAMGINEFIIPPSIDPLSEKNRQLSDDEINETAQKLG
                     IPRDRPVILQVSRFDRFKDPTGVIMAYRMVKKYNDCVLVLAGSPATDDPEGAGVLEEV
                     RRFASDDPDIHILLLPPFSDRDINALQTIASVILQKSLKEGFGLTVAEGMWKGKPVIG
                     GAAGGIPLQIVHGVTGFLVHSIEGCAFRIRQLLNNTEMAASMGEQAREFVRQNFLITR
                     QVRDYLAVWYTLLTNKKSLVEL"
     gene            1295..3154
                     /gene="treZ"
                     /locus_tag="PKJ17_02975"
     CDS             1295..3154
                     /gene="treZ"
                     /locus_tag="PKJ17_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HOD74962.1"
                     /translation="MRVFDKTSAPAHLGAVPSADGSTSFRVWAPFARTVSVDMLLGPG
                     GRREPLTPSGGNYFEGKVDGVGDGDLYRYVIDGSSTFPDPASQSQPEGVHGPSRIVDH
                     ASFRWSDDGWRGRALEEYIIYELHVGTFTEQGTFEAIVGCLDYFKDVGINAIELMPVS
                     QFPGSRNWGYDGAFPFAPHNTYGGPRGLKTLVNACHEQGFAVVLDVVYNHLGPEGNYL
                     ANFGPYFTDRHRTPWGEALNFDGPFSDEVRNYFIRNALDWFVRYHVDALRLDAIHGIF
                     DFSARTFLQELSETVGSALSGNPPAYLIAESDLNDIRIITPRENGGIGLDAQWNDDFH
                     HALHTLLTGERQGYYQDFGDIENLAKSFAEGYVYTGEFSSYRVRKHGSPSKHRPATQF
                     IVFSQNHDQAGNRPGGDRLASLVGHDRLKVAATAVLLSPYIPLLFMGEEYGETSPFLY
                     FVDHGDERLIEAVRTGRTREFAWFPSIGEIPDPQREKTFRLSRIDKVSRFEEARRPLF
                     AFYRHLTSLRRSLRPWQMTGGRPLVRCWPDKGTLFVMMPLPGNDVSLFFNFSEENAHV
                     ASPLHEGSWAAVSDTLSTRWGGSGEIAPPRICGKTGTVLSLGPLNAVAYRREG"
     gene            3177..>4656
                     /locus_tag="PKJ17_02980"
     CDS             3177..>4656
                     /locus_tag="PKJ17_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOD74963.1"
                     /translation="MERPVFRNLRIPLATYRVQFNRDFRFTDAVKVIPYLRELGITDL
                     YASPYFAARPGSLHGYDITDPTRINPEIGTREDYDIFVTTLREHDMGQILDIVPNHMC
                     IDGENRYWMDLLENGPSSPYSGFFDIDWDPVKEELHGKILLPFLGDQYGVVLEDRQIR
                     LEYRDGSFFVLLYEIRLPLLPETYSDILSFGIDHLGERIGETAGYEELLSIITSAGKL
                     PSYVETDPERAAERTREKEIVKKRLNGLYRSDGDIRAFVDRNIAVFNGNEGDPKSFDL
                     LDGLLSRQIYRLSHWQVATEEINYRRFFDINSLAAIRVEDPTVFDETHRFVFELIEGG
                     CVTGLRVDHPDGLYDPSEYFDRLQRRCFAIAMRSHLEEVRENVDLPYGRSYIEPAISE
                     RYDEALRTHRYFKPFYIVAEKILGKGEIMPEEWPLFSTTGYVFLNSLNGLFVDGRNAK
                     AFDTLYRRFTGMQTDFQDVLYENKKLVMEVAMSSEVNTLGHRL"
BASE COUNT          988 a         1435 c         1365 g          868 t
ORIGIN      
        1 gagcagggac atggaaaaga cgggggtcct gccatgatag gatcgtacgg cggcatctct
       61 ccccggggcg acctcctgct tcttcgaaag ttggccgacc atctggcggg aaagactttc
      121 ctccacatca attcgacccg tgagggcggg ggagtggcgg agatactcca gaggatgatc
      181 cccatcatgt caagcctcgg ggtcaacgcg cggtgggacg tcataacagg cgaccaaagg
      241 ttcttcgaca tgacgaagaa ggtgcacaac gccctccagg gatacacgga aaccatcgat
      301 gaccggatgt gggagcatca tttcgagatc aatgaggcaa acgcctccca catgaacctc
      361 gacgcagacg cggtgctgat acatgacccc cagccccttc ctctggtcac cttcaggaag
      421 gaaggcgtct ggatctggcg gtgccacata gacgtctcca acccccagag agatgtgttc
      481 gaaaagatag ggaagtacgc cacgaagttc gacgcggcca tctactccgt tgcccgattt
      541 gcgggcgcca tgggcatcaa cgagttcatc atacccccgt cgatcgaccc cctgtccgag
      601 aagaacaggc agctcagcga cgacgagatc aacgagacag ctcagaagct cggcatcccc
      661 cgggaccgac cggtcatcct ccaggtatcc cgtttcgaca ggttcaagga ccccaccggc
      721 gtcatcatgg cctaccgcat ggtcaagaag tataacgact gcgtcctcgt gctggcaggc
      781 agcccggcaa cggacgaccc cgaaggagcg ggggtgctgg aggaggtccg gcggttcgct
      841 tcggacgatc ctgacatcca catcctcctt ctcccaccct tcagcgacag ggacatcaac
      901 gcactgcaga ccatcgccag cgtcatcctc cagaaatcgc tcaaagaggg tttcggcctc
      961 accgtcgctg aaggcatgtg gaaaggcaag cccgtcatcg gaggcgcggc aggaggcata
     1021 cccctgcaga tagttcacgg cgtgacgggc tttctcgttc attccatcga ggggtgcgcc
     1081 ttcaggatac gccagctcct caacaacacg gagatggccg ccagcatggg tgaacaggcc
     1141 cgcgagttcg tccgccagaa cttccttatc acgagacagg tgcgggatta cctggcggtg
     1201 tggtacacgc tcctcacgaa caagaagagc ctcgtggaac tgtagggacg ggccgcggct
     1261 cgcgcctcac gatcttctgc tatactggtg agagatgaga gtattcgaca agacgagcgc
     1321 gccggcgcat ctcggagcgg tgccctccgc ggacggatcg acatccttcc gggtgtgggc
     1381 gccttttgcg cggacggtgt ccgtggacat gctcctgggt cccggaggga ggcgggagcc
     1441 cctcacgcct tccgggggga actacttcga aggaaaggtg gacggcgtcg gcgatggcga
     1501 cctttaccgg tatgtcatcg acggaagctc cacttttccc gatcccgcat cccaatcgca
     1561 gccggagggg gtccacggcc cctcgcggat cgtggaccac gcgtcgttcc gatggagcga
     1621 cgacggatgg cggggccgcg cccttgagga gtacatcatc tacgagctcc acgtgggcac
     1681 cttcacggag cagggaacct tcgaggccat tgttggctgc cttgattatt tcaaggacgt
     1741 gggtatcaat gccatcgagc tcatgcccgt cagccagttc ccgggcagcc ggaactgggg
     1801 atatgacggc gcctttccct tcgcccccca caatacgtac ggagggcccc gggggctcaa
     1861 gaccctggtg aacgcctgtc acgaacaggg cttcgctgtc gttctcgatg tggtctacaa
     1921 ccatctcggc cccgaaggca attacctggc aaacttcggg ccctatttca cggaccgcca
     1981 tcggacgcca tggggcgagg ccctcaactt cgacggcccc ttcagcgacg aggtgcgcaa
     2041 ctatttcatt cgaaacgccc tcgactggtt cgtacggtac cacgtggacg ccctcaggct
     2101 cgacgccata catgggatat tcgacttcag cgccaggacc ttcctgcagg agctctcgga
     2161 gacggtgggg tccgcgctgt ccggcaatcc ccccgcctac ctcattgccg agagcgatct
     2221 caacgacatc cggatcataa caccccggga gaacggcggc atcggcctcg acgcccagtg
     2281 gaacgacgat ttccatcacg ccctccacac ccttctcacc ggggagagac agggctatta
     2341 ccaggatttc ggcgatatcg agaatctggc gaagtctttc gctgaagggt acgtatacac
     2401 gggcgagttc tcctcctacc gcgtaagaaa acacggaagc ccgtcgaaac acaggccggc
     2461 cacgcagttc atcgtcttct ctcagaatca cgaccaggcg ggaaacaggc ctggcggcga
     2521 ccgcctcgcc tccctcgtgg gtcacgacag gcttaaggtc gcggcgacag ccgtcctgct
     2581 gtcgccgtac atccctcttc ttttcatggg ggaagagtac ggcgagacct ctcccttcct
     2641 gtatttcgtg gaccacggtg acgaaaggct catcgaggcc gtaaggacag ggagaacacg
     2701 cgaattcgcc tggttcccgt ccatcggaga gatacccgac ccccaaagag agaaaacatt
     2761 ccgtctgtcg aggatcgaca aggtctcccg cttcgaggag gcccgtcgcc cactcttcgc
     2821 attttaccgg cacctgacat cgttgcggcg ctcgctcagg ccctggcaga tgacaggggg
     2881 ccgccctctc gtcagatgct ggccggacaa gggcaccctc tttgtcatga tgcctcttcc
     2941 agggaatgat gtttccctct ttttcaactt cagtgaagag aacgcgcacg tcgcatcgcc
     3001 gctccacgaa ggctcgtggg ccgcggtctc cgacaccctt tcgacccgtt ggggcggaag
     3061 cggggaaata gcaccgcccc gcatctgcgg gaagaccggg accgtccttt ccctggggcc
     3121 gctcaacgcc gtcgcgtaca ggagagaggg atgatggggc atgaggtgag ctagccatgg
     3181 aaaggcccgt ctttcgaaac ctccggatac ctcttgcgac ctaccgggtc cagttcaacc
     3241 gggatttccg gttcaccgac gcggtgaagg tgatccccta cctcagggaa ctgggtatca
     3301 ccgacctcta tgcatccccc tattttgctg cccgtcccgg gagccttcac ggatacgata
     3361 tcacggatcc caccaggatc aacccggaga tcggcacccg ggaagattac gatatcttcg
     3421 tgacgaccct gcgggagcac gatatgggac agatcctcga catcgttccc aatcacatgt
     3481 gcatcgacgg agagaaccgg tactggatgg atcttctgga gaacggaccg tcctccccgt
     3541 acagcggctt cttcgacatc gactgggacc cggtcaagga ggagctgcat ggcaagatcc
     3601 tcctgccctt tcttggcgac cagtacggcg tcgttctgga ggacaggcag atacggctgg
     3661 aatacaggga cggctcattc ttcgtcctcc tctacgagat caggctgcct cttcttccgg
     3721 agacgtacag cgacatcctc tccttcggga tcgatcacct cggggagagg atcggcgaaa
     3781 cggccggtta cgaagaactg ctcagcataa tcacgtcggc aggaaaactg ccttcgtacg
     3841 tcgaaacgga cccggaaagg gcagccgagc ggacccgcga gaaagagatc gtgaagaaac
     3901 ggctgaacgg tctctatcga agcgatgggg acatacgggc cttcgtggac aggaacatcg
     3961 cggtcttcaa tggcaacgag ggtgacccga agagcttcga ccttcttgac ggtttgctct
     4021 cgcggcagat ctaccggctg tcccactggc aggtggcgac ggaagagatc aactaccgga
     4081 ggttcttcga catcaacagc ctagccgcca tcagggtcga ggacccgacg gtctttgatg
     4141 agacgcaccg ctttgttttc gaactcatcg agggcgggtg tgtcacgggc ctgagggtgg
     4201 accaccccga tggcctctat gacccctccg agtatttcga ccgcctccag cggcgctgct
     4261 ttgcgatagc catgagatcg cacctcgagg aggtcaggga gaacgtcgat ctcccctacg
     4321 gcaggtctta tatagaaccc gccatctcgg aacgctacga cgaggcgttg aggacacatc
     4381 gatacttcaa gcccttttac atcgttgcag agaagatact cgggaagggt gagatcatgc
     4441 cggaggagtg gcctctattc agcaccacgg gatacgtgtt cctgaactcc ttaaacgggc
     4501 tcttcgtcga cggacggaac gcgaaggcct tcgacacgct ttacaggcgg ttcacgggaa
     4561 tgcagaccga tttccaggac gtgctctacg agaacaagaa gctcgtcatg gaagtcgcca
     4621 tgtcgagcga ggtcaacacc ctgggacacc gcctga
//
LOCUS       DAOPOA010000091         4571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_20551_length_4571_cov_3.964792, whole genome shotgun sequence.
ACCESSION   DAOPOA010000091 DAOPOA010000000
VERSION     DAOPOA010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20551_length_4571_cov_3.964792"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKJ17_02985"
     CDS             complement(<1..788)
                     /locus_tag="PKJ17_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOD74964.1"
                     /translation="MITLKIDGKEIKTEKGKTILEAARENDIHIPTLCYHENLLPIGS
                     CRLCIVEIEGYDKPVASCTTAAVEGISVTTRSEKLFAMRQEYPRFLLINHPLDCPICD
                     SGGECRLQDLVFEHKIESVDLAAEREPKKVKPYATALIRYSEDRCVLCLRCVHACREI
                     SGRRVLELTGSGIEAAMAPKVPEDCISCGECLNVCPVGALTEQESPLKSRKWQTKRQT
                     TTCPHCGFGCSLDLDVFEDRFVTKVVTRPERLPNKGSLCVMGRFG"
     gene            complement(802..2751)
                     /locus_tag="PKJ17_02990"
     CDS             complement(802..2751)
                     /locus_tag="PKJ17_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD74965.1"
                     /translation="MADVIFSSWQGEIIDNRGKARESFEAPKVKLPGELEKGLPVKAF
                     MGWDGIILCDESVDIVDMSVRYVEAVQKESCGRCVPCRIGSKVILDILKGIADGRGKK
                     EDLARIPTIAAGIKDGSKCQIGQTGFVPLLQALSFFGPEFDRSIAEGRKAKAGDYKVS
                     VTAPCMSVCPTQLDIPKYVEAISEGRFTESLATIRENTCLAGTLGRVCVRPCESNCRR
                     ANIDEAISIKSLKRFVADYELDKDVKRNVEAKIDKNKKIAVIGAGPAGLSCAYYLAQM
                     GYPVTVFEKLGEPGGMAAVGIPDYRLPRVILGGEGAFVESFGVTMRYGVALGKDITID
                     QIRKDFDAVFIGVGAHSSSPMGVKGEDMGYRGFIPGVKYLLDINNGEDPYPEGKKVVV
                     VGGGNVAMDCVRSSFRVGKPDVHLVYRRTKKEMPADPVEIHEAEEEGVEFHYLCNPTR
                     ILEKDGKVVGVECIRMELGEPDASGRRRPVPVPGSEFVIDTDILIPAIGQAVDFSFLE
                     KKEDFAITKWNTFQVDQETFETNVPGVFSAGDCETGPDVLVRACGNGKRAAWKIDEYL
                     RGEKPKARMSERFVKFFGDVKVYDKNENVGFLGDRARLLLRPMAPEVRKWTFDEVEEG
                     FRTDEAITEASRCLRCYRIGMIAVG"
     gene            complement(2761..3933)
                     /gene="nuoD"
                     /locus_tag="PKJ17_02995"
     CDS             complement(2761..3933)
                     /gene="nuoD"
                     /locus_tag="PKJ17_02995"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HOD74966.1"
                     /translation="MAETKYMTINMGPQHPATHGVLRVVLELDGETVVSSIPDIGYLH
                     RGIEKIFENRTYWQGQPLTDRLDYTSGMSNNLAYCLAVEKLLGIEIPKRAQYIRVMLS
                     ELQRIAAHLLWISTHALDMGAMTVLFYGFRERESCLKVLETVAGARLMPDYIRFGGLR
                     EELPEGFLKEAKAFIDDLPRRIDEYETLLTENVIWKRRLKGVCPLSAEDAIGYGVTGP
                     VLRASGVNYDVRKAYPYSSYEDFDFEIPLGTAGDVYDRYLVRLKEMRQSVRIVDQAIQ
                     KLPEGPIKADAPEYVPPEKEEVSKDMAALIRHFKIMADGIKPPKGEAYASIESSKGEL
                     GFYIVSDGSEKPYRVRIRPPSFLNLGSVSKMIEGSLVADVVAAIGSIDIVLGEIDK"
     gene            complement(3934..4425)
                     /locus_tag="PKJ17_03000"
     CDS             complement(3934..4425)
                     /locus_tag="PKJ17_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOD74967.1"
                     /translation="MSDNSEFLKILSDKVAGVKIVPTPPGEPSAMVDRESIHEALDHL
                     RYDRDLLFDMLVELFAVDYTGEEPRFEIVYILRSTKNNARIVVKTRTGDEGIETASHL
                     WPAANFLEREIYDMFGLPFANHPDLRRIYNPDDFEGHPLRKEFPLEGKDFDKPFVVDL
                     EKA"
BASE COUNT          853 a         1421 c         1267 g         1030 t
ORIGIN      
        1 ccgaagcgtc ccatcacgca gagggaaccc ttgttgggaa gacgttccgg acgggttacg
       61 accttcgtca cgaacctgtc ctcgaacacg tcgaggtcga gggaacagcc gaatccgcag
      121 tgcgggcagg tcgtcgtctg ccgcttcgtc tgccacttcc tgctcttcaa cgggctttcc
      181 tgctcggtaa gggcgccgac gggacacacg ttgagacatt caccgcagga gatgcagtcc
      241 tcgggcacct tcggcgccat ggccgcttct ataccgctcc cggtaagttc caggaccctg
      301 cgaccggata tctcacggca ggcatgaacg cagcgcagac agagcacgca tctgtcctcg
      361 gaatacctga tcagcgccgt ggcatagggt ttcacctttt tcggctccct ttcggcggca
      421 agatcgacgg attcgatctt gtgctcgaag acgaggtcct gcagacggca ctccccgccg
      481 gagtcacaga tgggacagtc gagggggtga ttgatgagaa ggaacctcgg gtactcctgg
      541 cgcatggcga agagcttctc cgatcgtgtc gtgaccgata tgccctcgac ggcggccgtg
      601 gtgcacgatg ccaccggttt gtcgtacccc tcgatctcaa cgatgcaaag cctgcacgag
      661 ccaatgggga gaaggttctc gtggtagcac aacgtgggaa tgtggatgtc gttctcccgt
      721 gccgcttcga gtatcgtctt acccttctcg gtctttatct ctttgccgtc tatcttgagt
      781 gttatcattc ccgttccctt ttcaaccaac cgcaatcatg cctattctgt aacagcgaag
      841 gcaccgtgac gcctccgtga tcgcctcatc ggtccggaaa ccctcttcca cctcgtcgaa
      901 ggtccacttc ctgacctcgg gggccatcgg ccgcagaaga agtcttgccc tgtcgcccag
      961 aaaaccaacg ttctcgttct tgtcgtagac cttgacgtca ccgaagaact tcacgaacct
     1021 ctcgctcatg cgggccttcg gtttctctcc cctcaggtat tcatcgatct tccaggcagc
     1081 gcgcttgccg ttgccgcagg cgcggacgag gacgtccggg ccggtttcgc agtcgccggc
     1141 ggagaaaaca cccggcacgt tggtctcgaa ggtctcctgg tcgacctgga aggtgttcca
     1201 tttcgttatg gcaaaatctt ccttcttctc aaggaaggag aagtcgacgg cctgaccgat
     1261 cgcggggatg agtatgtccg tatcgatcac gaactcggaa cccggaacgg gaaccggcct
     1321 tctgcgccca ctcgcgtccg gttctcccag ttccattcgg atgcactcca cgccgacgac
     1381 cttgccgtcc ttctcgagga tccgcgtggg attgcacagg tagtggaact caacgccctc
     1441 ttcctccgcc tcgtggatct ccacggggtc cgcgggcatc tctttcttcg tacgcctgta
     1501 gaccaggtga acgtccggct tgccgacgcg gaaggatgac ctcacacagt ccatggccac
     1561 gttgccgccg ccgacgacga cgaccttctt tccctcggga tacgggtctt ccccgttgtt
     1621 gatgtcgaga agatacttca cgccggggat gaagccgcga taccccatgt cctcgccctt
     1681 aacccccatg ggtgaagagc tgtgagcgcc gacaccgatg aagacggcat cgaaatcctt
     1741 ccgtatctgg tcgatggtta tgtctttgcc cagagccacg ccgtaccgca tggttacgcc
     1801 gaaggactcc acgaaggcgc cctcgccgcc aagaatgacc ctcggcaaac gatagtcggg
     1861 aatccccacc gccgccatgc cgcctggttc cccgagcttc tcgaagaccg tcacggggta
     1921 gcccatctgg gcgagataat aggcgcagga aagcccggca gggccggcac cgatgacggc
     1981 gatcttcttg ttcttatcta tcttcgcctc gacgttgcgt tttacgtcct tgtccaattc
     2041 gtagtccgca acgaaccgct tgaggctttt gatggagatc gcctcgtcga tgttcgctcg
     2101 cctgcagttg gactcgcacg gccggacaca gacccttccc agtgtcccgg cgaggcaggt
     2161 gttctcccgt atcgtcgcga gggattccgt gaaccgccct tcgcttatgg cctccacgta
     2221 cttcgggata tcgagctgag tggggcacac gctcatgcag ggggccgtga cggacacctt
     2281 gtagtcgccc gccttcgcct tgcgcccttc agctatcgac cggtcgaact cggggccgaa
     2341 gaacgaaagg gcctgaagga gaggcacgaa acctgtctgg ccaatctggc acttggagcc
     2401 gtcctttatg ccggcggcga tggtggggat cctcgcaaga tcttccttct tgccccgacc
     2461 gtcggcgatg cccttcagga tgtccagaat gaccttgctg cctatcctgc agggcacgca
     2521 ccgtccgcag gattctttct ggaccgcctc gacgtacctg acggacatgt cgacgatatc
     2581 cacactctca tcacagagga tgatgccatc ccaccccatg aaggccttga cgggaagacc
     2641 cttctctaac tctccgggga gttttacctt cggggcttcg aaagactcgc gggccttgcc
     2701 cctgttgtct attatctcac cctgccagga actgaagatc acgtctgcca tagtcaacct
     2761 ttacttgtct atttccccga gaactatgtc gatgcttcct atcgcagcga ccacgtcggc
     2821 caccagcgat ccctctatca tcttcgacac ggagccgagg ttgaggaatg agggcggacg
     2881 gatccttacc ctgtagggtt tctccgaacc gtcgctgacg atatagaacc cgagctcacc
     2941 ctttgaagac tcgatcgacg catacgcctc acccttcggc ggcttgatgc cgtcggccat
     3001 gatcttgaaa tggcggatga gggcggccat gtcctttgac acctcttcct tttccggtgg
     3061 cacgtattcc ggcgcatccg ccttgatggg cccctcgggg agcttctgga tggcctgatc
     3121 gacgatcctt acggactgtc tcatctcctt gagacggacg aggtaccggt cgtagacatc
     3181 acctgccgtg ccgagaggta tctcgaagtc gaaatcctca tagctcgaat agggataggc
     3241 tttacggacg tcgtaattaa cccccgaggc gcgaagcacg gggcccgtca cgccgtagcc
     3301 tatggcatcc tcggcgctca agggacagac acctttcagg cgcctcttcc atatgacatt
     3361 ctccgtaagc agtgtctcgt attcatcgat cctgcggggg agatcgtcga tgaaggcctt
     3421 cgcttccttc agaaaaccct cgggcagctc ttcccggagc ccgccgaagc gtatatagtc
     3481 cggcatgagc cttgcgcccg caactgtctc caacaccttc aggcaggatt ccctttcccg
     3541 gaacccgtag aaaagcaccg tcatggcgcc catatcgagg gcgtgcgtcg atatccagag
     3601 aaggtgggcg gcgatgcgct gcaattcact cagcatcaca cggatgtact gggccctttt
     3661 cggtatctct atgccgagca gtttctccac ggcaaggcag taggccaggt tgttggacat
     3721 gccgctcgtg taatcgagcc ggtccgtgag gggctggccc tgccagtagg tcctgttctc
     3781 gaagatcttc tcaatgccgc gatggagata cccgatgtca gggatgcttg acacgacggt
     3841 ctcgccgtca agctcgagca ccaccctcag caccccgtgt gtggcgggat gctgcggccc
     3901 catgttgatc gtcatgtact tggtctcagc catttatgcc ttctccaggt ccaccacgaa
     3961 gggtttgtcg aaatccttgc cttcgagggg aaactccttg cgcaggggat ggccctcaaa
     4021 atcatcgggg ttgtatatcc tgcgcagatc gggatgattc gcgaaaggca gaccgaacat
     4081 gtcgtagatc tccctttcga ggaaattggc agccggccac aggtggctcg ccgtttcgat
     4141 gccctcgtcg cccgttctcg tcttcaccac tatcctcgcg ttgttcttcg tggagcggag
     4201 gatgtagacg atctcgaagc gcggctcttc gcctgtgtag tcgactgcaa agagctcgac
     4261 aagcatgtcg aacaagaggt cccggtcata cctgagatgg tcgagggcct cgtggatgct
     4321 ttccctgtcg accatcgcgc tcggttcgcc ggggggcgtc ggcacgatct ttacgcctgc
     4381 caccttatcg gagagtattt tcagaaactc gctgttatcg ctcatgcact cttcccgggc
     4441 aatgttatca gaggtctgtc cttgatggac tctttctgga tcttcttctg aagctctatg
     4501 atgcccttca ggagggcttc cggccgggga ggacatccgg ggatgtacac gtccacgggg
     4561 attatctggt c
//
LOCUS       DAOPOA010000092         4570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_20559_length_4570_cov_3.610631, whole genome shotgun sequence.
ACCESSION   DAOPOA010000092 DAOPOA010000000
VERSION     DAOPOA010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20559_length_4570_cov_3.610631"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PKJ17_03005"
     CDS             <1..608
                     /locus_tag="PKJ17_03005"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOD74968.1"
                     /translation="LRGKDIRLRIFRKGHDTGGSRYDKDDVTMTAAAYDFIRKHDDGQ
                     FFGTKGMSEQKRSGKHIDIKPIDRFPGPNGKIIPGGLSLALLDTDYFKGIVEMRLAAE
                     PDKFGRFTFHSETREDFINHLTAEEKRRNQKTRKWEWVKVRSGNHWLDCVVIAFAMAD
                     DECLGGIRVLADPMEEITGEVIGPSDDTGDWITGGKGGSWL"
     gene            919..1194
                     /locus_tag="PKJ17_03010"
     CDS             919..1194
                     /locus_tag="PKJ17_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74969.1"
                     /translation="MARTKKPTHQKAKPMRQSINTTQADVEVRDPVLDASEPEIPETT
                     PETAPDLPRYQDGCPECHERSLVTELKIRSWRQCRCRECGWRGEMED"
     gene            1198..1431
                     /locus_tag="PKJ17_03015"
     CDS             1198..1431
                     /locus_tag="PKJ17_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74970.1"
                     /translation="MSTFTTWSALKTQLLNDLASRNFAVEEYVTPAGVKVKARSLAEI
                     KNFIEFCDVMIAAEAGESGTVAYAQFNKPGCGI"
     gene            1428..3044
                     /locus_tag="PKJ17_03020"
     CDS             1428..3044
                     /locus_tag="PKJ17_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOD74971.1"
                     /translation="MNDKGPTAFDRFISYFAPGYALKRYSAHLMFKALSGGDKGERSF
                     EAVSSSRLRGDWTNITQDADAALGTSGPALRNMVRDLSHRSGLVAGPLRRITNNVIGL
                     GIRPQARVKVDGDFDAPPGTEKITENTAKRFNYQAEKTYRRWTKKASSDLRTTMEELQ
                     ALAFRGMIGDGETLVVVRSSSKKGRIIPLALEVIEIDRLSTPPGEIKNPKVRNGILFD
                     DEGAPQTYYVLKRHPGSTNVQTIAGTNDYEEIAAYGSNGLKKVIHLYDILRPGQSRGY
                     SPFAAGLGDMQDLARYREAVIVAARVGACLAAFIERPNSFTNAQQNPSSTSRQLITKF
                     EPGMIKYTNPGEKVTPFTPSQPQNQFGEFTKQILMTAANAVDVPYEVFANDWAGLNYS
                     NARTVLLQAYLAFRIYQRFMINHLCIPVWENFVSDCVAVGKVEAPGFGVRKEDYFEVS
                     WVPPGWQWVDPLKESQAATNDIDNLITTLSDTLAGRGDDWEEKLEQRARELKRISELE
                     DKYEIKMKPDKSPAVKAGGDKEAGGKKDEE"
     gene            3034..3954
                     /locus_tag="PKJ17_03025"
     CDS             3034..3954
                     /locus_tag="PKJ17_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74972.1"
                     /translation="MKNDLFYRNVDIEERAAENSRAVDLSFSSEIGVQRWFGVEILCH
                     KPGAMKKDRLNTLLFAHNRERIVGPVSNVRVQGGVGRATVGFDETDEGNLALARVNSG
                     SLRTTSVGYQVHKFRKLGEGEEYQLASRTVKGTPDNNPTYIAENWSPMEISLEPTPAD
                     PSVGIGRCATRSLDGIDIEEPPVIETAVPAPATESRTGGNSEMDEKELQTRVDEALAR
                     QTEASRAAMKKVFDRAAAAGQEGLAFRMYVEGRTEDEITDAIIAAQAKERGTGGDAGE
                     GEGADKRTLDKIDDETLIRGLTSPELMVLE"
     gene            3966..>4570
                     /locus_tag="PKJ17_03030"
     CDS             3966..>4570
                     /locus_tag="PKJ17_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74973.1"
                     /translation="MTAVNKSPWIRSLVNAPATIFPGKVQAGSTQAIKKGELCTYDET
                     SGYFIPVDAVADCRYSLAIANEEQKADSLERYMEFLALCPWDVFEFALDASAQIVLGD
                     GLGLTASDSQKLTRDVDGNAVAFYVGTDNYPEVGTTMLYRTHGQVVFNPVHSYWMQRA
                     LKNNLKKVISTAASITLKPEDCGAVVLVTDTATITLPSGNVP"
BASE COUNT         1142 a         1228 c         1352 g          848 t
ORIGIN      
        1 agctcagggg caaggacatc cggctcagga tattccgcaa ggggcatgac acaggcggct
       61 cccggtacga caaggacgat gtgacaatga ccgccgcggc ctacgacttc attcggaaac
      121 acgatgacgg gcagttcttc ggcacaaagg ggatgtccga acagaagagg tcggggaagc
      181 acatcgacat taagcccata gaccgctttc ctggcccgaa cggcaagatc atccccgggg
      241 gcctttccct tgcccttctc gacacggact atttcaaggg aatagtggag atgcgcctcg
      301 ctgcggagcc tgacaagttc ggccgtttta ccttccattc cgagacgaga gaagatttta
      361 tcaaccattt gaccgcggag gagaaacgca ggaaccagaa aacgcgcaaa tgggagtggg
      421 tcaaggtcag gtcgggcaac cactggcttg actgcgtggt gattgccttc gccatggccg
      481 acgacgaatg cttgggaggc atcagggtct tggctgaccc catggaggag ataacgggcg
      541 aggtcatagg gccgagcgat gatacaggcg actggataac gggagggaag gggggatcat
      601 ggctgtagac cggttcaagg accttccaag taagagcctt ctgagacccg atgaagtggc
      661 tgagtttttc agcgtcagca agcgcacaat ctacctttgg gtggaaatgg ggaagcttga
      721 gggggtcaaa cccgtcagtg gcatacttcg aatattccgc gagtcagtgg taaaactagc
      781 aaaatcatag atatagaccc gttctgcatc aaaaaatagt gtgaaatccg tgcagttttg
      841 tgtcgtagcg tcttagacgg ggcgccgggg cttcggtaat ctctccccat agcatcaaat
      901 acctctcgag gagagatcat ggcacggaca aagaaaccga cccatcagaa agcgaagccg
      961 atgaggcaga gcatcaacac cactcaggcc gatgtggagg tgagggaccc cgttcttgat
     1021 gcctcagagc ctgaaatccc cgaaacgacc cccgaaacag cccccgatct tccccgctat
     1081 caggacggtt gcccggaatg ccatgaacgc tccctcgtaa cggaactgaa gatcagatca
     1141 tggcgccagt gccgttgccg tgaatgcgga tggcgcgggg aaatggagga ttaagtattg
     1201 tcaaccttca cgacatggtc ggccctgaaa acccaactcc taaatgacct cgcaagccgg
     1261 aatttcgcag tcgaggagta cgtcaccccc gcgggggtga aagtgaaggc cagaagtctc
     1321 gccgagatca agaacttcat cgagttctgc gacgtgatga tcgccgccga agcgggagaa
     1381 agcggaaccg ttgcctatgc ccagtttaac aagccggggt gcggaatatg aacgacaagg
     1441 gacctactgc ttttgaccgg ttcatttcct atttcgcccc gggttatgcc ctgaaaaggt
     1501 actcggctca cctcatgttc aaggcgctgt ccggcggcga taaaggtgag cggtcattcg
     1561 aagccgttag cagctcccgt ctcagggggg attggacgaa catcacccag gacgccgatg
     1621 ccgcgctcgg aacgagcgga cccgccctga ggaacatggt aagggacctc agccatcgca
     1681 gcggtctcgt tgccggtcct ctcagaagga taacaaacaa cgtcattggt ctcggaatca
     1741 ggccacaggc ccgcgtgaag gtcgacgggg actttgatgc tcctcctggt accgagaaga
     1801 tcaccgaaaa taccgccaaa aggttcaact atcaggctga gaagacttat cggcgatgga
     1861 ccaagaaagc atcttctgac ctgaggacca ccatggagga actccaggcg ttggcattca
     1921 gggggatgat cggcgatggt gaaacgctcg tcgttgtgag gtccagttcc aagaaggggc
     1981 ggataatacc gctggccctc gaggtgatcg agatcgacag gttgtcgacg cccccgggag
     2041 agataaagaa cccgaaagtc aggaatggca ttctctttga cgatgaagga gccccgcaaa
     2101 cctattacgt tctcaagcgc cacccggggt cgacgaacgt ccagacgatc gcgggtacga
     2161 atgactatga ggaaatagca gcctacgggt ccaacggcct caagaaggtc atccaccttt
     2221 acgacatcct gaggcccggg cagtccagag gctattctcc tttcgccgca ggtctcggag
     2281 atatgcagga ccttgcccgt tatcgtgagg cggttatcgt ggccgcgcgc gtcggggcct
     2341 gtcttgcggc cttcatcgag cgccccaatt ctttcacgaa cgcccaacag aacccgagca
     2401 gcacgagcag acagcttatc accaagttcg agcccggcat gatcaaatac acgaaccccg
     2461 gggaaaaagt gacacccttt accccgagtc agccgcagaa ccagttcggg gagttcacca
     2521 agcagatcct catgacggcc gcgaacgccg tggatgttcc ctacgaggtg tttgcgaatg
     2581 actgggcggg gctcaattac tccaatgcca ggaccgttct ccttcaagcc tatctggcct
     2641 ttagaatcta tcagcggttc atgatcaatc acctgtgcat ccccgtttgg gagaatttcg
     2701 tgtccgattg tgtggccgtg ggcaaggtgg aagcccctgg tttcggcgtc cgcaaagagg
     2761 actatttcga agtttcatgg gtgccacctg gctggcagtg ggtcgatccc ttaaaggaat
     2821 ctcaggcggc gacgaacgac atagacaacc tgataacaac cctttccgat acacttgccg
     2881 gccgcggtga cgactgggaa gagaaactcg agcagagagc gcgggagctc aagcgtatat
     2941 ccgagctcga ggacaaatac gagatcaaga tgaaaccgga taaatcaccc gcggtaaaag
     3001 caggcgggga taaagaagca ggaggcaaga aagatgaaga atgacctgtt ttaccggaac
     3061 gtggacatag aggaaagagc ggccgagaat tccagggccg tcgatctcag cttttcctct
     3121 gaaattgggg ttcagcgctg gttcggcgtt gagatactct gccacaagcc cggagccatg
     3181 aaaaaggacc gtttgaacac gcttctcttt gctcacaacc gggagaggat tgtcggtccg
     3241 gtctcaaacg tgcgtgtgca gggcggtgtc ggccgggcga cggtcggttt tgacgagacc
     3301 gacgagggca atctcgcact tgcccgtgtt aacagcggtt cactgaggac aacgtctgtc
     3361 ggctatcagg tccacaagtt ccgcaagctc ggtgagggcg aggaatatca actggcatcg
     3421 cggaccgtga agggcacgcc tgataacaac ccaacctata ttgccgagaa ctggtctccg
     3481 atggagatca gccttgagcc gactccggcc gatccgtctg tgggtatagg ccgttgcgcg
     3541 acccgttcat tggacgggat cgatatagag gaaccgcccg tcatcgagac ggcggtcccg
     3601 gcaccagcaa cagaatccag aacaggaggt aacagcgaga tggatgagaa agagcttcag
     3661 acaagagtgg atgaagccct tgcgagacag acggaagcga gtcgcgcagc aatgaagaaa
     3721 gtattcgacc gcgccgccgc cgcagggcag gaggggcttg ccttcaggat gtacgtggag
     3781 ggcaggaccg aagacgagat caccgacgcg atcattgcgg cacaggcgaa ggagagggga
     3841 acgggaggag acgctggaga aggcgaaggc gccgacaaga gaaccctcga caagatcgat
     3901 gacgagaccc tgatcagagg ccttacgagc cctgagctga tggtccttga gtaaggaggc
     3961 gcgacatgac agcagtcaac aaatcaccgt ggataagaag cctcgtcaat gcccctgcaa
     4021 ccatttttcc cggaaaagtg caggcaggca gcacgcaggc gataaagaaa ggcgaactct
     4081 gtacctatga cgaaacgagc ggatatttca tccccgttga tgcagttgcg gattgcaggt
     4141 actccctcgc gatcgccaac gaggagcaga aggccgacag tctcgaaaga tacatggagt
     4201 ttctggcgct ctgcccctgg gacgttttcg aatttgccct cgatgcctcg gctcagatag
     4261 ttctcggaga tggtcttgga ctgaccgctt ccgacagcca gaagctgaca agggacgtgg
     4321 acggcaacgc cgtggccttt tatgtcggca ctgacaatta cccggaagtc ggcacgacca
     4381 tgctctacag gacccacggg caggttgttt tcaaccccgt tcactcctac tggatgcagc
     4441 gggcgctcaa gaacaacctg aaaaaggtca tctccaccgc cgccagcatt accctgaaac
     4501 ccgaggattg cggggccgtg gttctcgtca ccgacacagc gactataacc cttccttccg
     4561 gcaatgttcc
//
LOCUS       DAOPOA010000093         4570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_20560_length_4570_cov_3.568771, whole genome shotgun sequence.
ACCESSION   DAOPOA010000093 DAOPOA010000000
VERSION     DAOPOA010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20560_length_4570_cov_3.568771"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PKJ17_03035"
     CDS             <1..642
                     /locus_tag="PKJ17_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD74974.1"
                     /translation="AVVAVIPIAYAIERFVIRKLYGVSIDYAIIATYAVVLIGVDSIK
                     WIWGASPIPLSDPIGKYVNIFSVTLPMYRVLIIILALVIFGVLTVFFKKTIVGKIVTA
                     SLEDKDAVKSLGINVDKYFSIVFVIGSCLAALGGVLYAPITSVHPYMGMMVLLISFAV
                     VLVGGLGNIKGTFVSAFVLGLIMAITGRYWGPAAETMVFVVMGIVLIFKPVEV"
     gene            999..2330
                     /locus_tag="PKJ17_03040"
     CDS             999..2330
                     /locus_tag="PKJ17_03040"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOD74975.1"
                     /translation="MTPEKRRDAIVDIFRAGVRAVDPARSVKEHMPYVLDTCRERGVT
                     EVEVIAFGKGAATMAKAAMEELNHRVHARGVVLTKYGHAHGVAFHGDVEVFEAGHPIP
                     DEAGHAAAGKVLKVLEDADETMLVLFLISGGGSALLTSPAEGMSLADKQAVTDLLLKS
                     GASIDELNRVRKHLSRVKGGRLAKAAYPAHGISLILSDVIGDRLDVIASGPTAPDPTT
                     FGDALAVLGKYDLMDKVPANVLKILHHGATGTLPDTPKRDDPVFANFENIIIAGNQKA
                     TAAAEARAAELGFNAVAPANNVEGEASKLGVRYARAIRSTQKWITNDPARQSMCFIYG
                     GEPTVTVKGNGKGGRNTELALAFAIAADGMEGVTFLSAGTDGTDGPTDAAGAIVDGGT
                     VKRGREKGLDAREYLERNDSYSFFKETGELLITGPTGTNVMDLEIVLIERP"
     gene            complement(2436..2774)
                     /locus_tag="PKJ17_03045"
     CDS             complement(2436..2774)
                     /locus_tag="PKJ17_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOD74976.1"
                     /translation="MFRFVSIALFAVSIVCMGATNASSARYDKLAGKATYDATCAMCH
                     KAGMMGAPKVGDKAAWAARIKQGMDVMVTRAINGFRGKKGMMPKKGGNPKLTDAQVGN
                     AVAYMVSRSK"
     gene            complement(3243..3752)
                     /locus_tag="PKJ17_03050"
     CDS             complement(3243..3752)
                     /locus_tag="PKJ17_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HOD74977.1"
                     /translation="MAKIAVIIADMFEDSEYTEPAKAFREAGHALVHIGLSAGKTVTG
                     KASGTAVKVEKAVKDVYADEFDALLIPGGYSPDKLRVDADAVRFAKEFVESGRPVFVI
                     CHGPQLLITANVLKGKKITGWKSIVQDIINAGADFEDRELVEDGNLISSRSPADLPAF
                     IKASLKKLA"
     gene            4107..>4570
                     /locus_tag="PKJ17_03055"
     CDS             4107..>4570
                     /locus_tag="PKJ17_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74978.1"
                     /translation="MKAGGKRDWIFIGAVIVLVTALHFLVLARHSPLVVLEELYYVPL
                     LLGALRFGLKGALVVYVLVSVLHIPFFFGEWTASPIGVADRVLHFVFSGIFAFTAGFL
                     VDRERRKREQAERERYLAGVGMAATTIVHDLRNQLHLILGYGLRIQKGKGDI"
BASE COUNT          969 a         1320 c         1297 g          984 t
ORIGIN      
        1 gcggtggtgg ccgtcatccc catcgcctac gcgatagagc gcttcgtcat ccgcaagctc
       61 tacggggtct ccatcgacta cgccatcata gccacctacg ccgtcgttct catcggcgtc
      121 gacagcatca agtggatatg gggcgcctcc cccatacccc tgtcggaccc catcgggaag
      181 tacgtgaaca tcttctccgt gacgctgccc atgtacaggg ttctcatcat catcctcgcc
      241 ctcgtcatct tcggcgtcct cacagtattc ttcaagaaga cgattgtcgg caagatcgta
      301 acggcatccc tggaggacaa ggacgcggtc aagagcctcg gcatcaacgt ggacaagtat
      361 ttctccatag tcttcgtcat aggcagctgt cttgcggccc taggcggggt gctctacgct
      421 cccatcacgt ccgtgcaccc ctacatgggc atgatggtcc tcctcataag ctttgccgtc
      481 gtccttgtcg gcggtctggg gaacatcaag ggcaccttcg tatccgcctt cgttctcggc
      541 ctcatcatgg ccataacagg ccgctactgg ggcccggctg cggagacgat ggtcttcgtc
      601 gtgatgggca ttgtcctgat attcaaaccc gtggaggtat gagcacgcaa agatacctcc
      661 agactgctcc ataacactac ccccccttcc gctggccacg ggacacccgc aagtcaagac
      721 tatccaaacc ccaacccgtg gtcagcggat ccctccagaa aagatggctt gaaccagaaa
      781 agacggcttg agccgcttaa accgcttgaa cgttgaagaa taggcaaata gctgctcgta
      841 tctttgacct ggtgcgaata cggttgcccc agcctgtcgg agatgtgggg gctcattttt
      901 ttgtttctca cgcttaagcc gctcaagcgg ttcaagccat tcaagccgtc ttatgtttat
      961 gtcttgaact tggtctttta gtgggtatac tagttactat gacaccagaa aagagaagag
     1021 acgcgatcgt tgatattttc agggcagggg ttcgggctgt tgatccggcg cggtcggtga
     1081 aggagcatat gccctacgtg cttgacacat gccgggaacg gggcgtgacg gaggtggagg
     1141 tcattgcctt cggcaagggt gccgccacca tggcgaaggc ggccatggag gaactcaacc
     1201 acagggttca cgcaaggggg gtcgtgctaa ccaagtacgg ccacgcgcat ggtgttgcct
     1261 ttcatgggga cgtggaggtc ttcgaagcgg gccacccgat accggacgaa gcgggccacg
     1321 cggcggcggg gaaggtcctc aaggttcttg aggatgccga cgagaccatg ctcgtcctct
     1381 tcctcatctc cggcggcggc tcggcgctcc tcacgtcccc cgcggagggt atgtcccttg
     1441 ccgacaagca ggccgtgacg gacctcctgc tgaaatccgg cgccagcata gacgagctca
     1501 acagggtacg caagcacctt tcacgcgtga aaggcggcag gctggcgaag gccgcctacc
     1561 ccgcccacgg aatatcgctc atcctctccg acgtgatagg cgaccgcctg gacgtcatag
     1621 cctcgggccc caccgccccc gaccccacaa cattcgggga tgcccttgcc gtcctgggaa
     1681 aatacgatct catggacaag gtccccgcca acgttctgaa aatactgcac cacggcgcca
     1741 ccggcaccct gcccgatacc ccgaagaggg acgaccccgt cttcgcgaac ttcgagaaca
     1801 tcataatagc cggcaaccag aaggccacag ccgccgcgga ggcccgcgcc gcggaactcg
     1861 gcttcaacgc cgtagccccc gccaacaacg tggagggcga agcaagcaag ctgggcgtcc
     1921 gctacgcccg cgccatcagg tccacccaga aatggataac caacgacccg gcccgccaga
     1981 gcatgtgctt catctacggc ggcgagccga cagtaacggt aaaagggaac ggaaaagggg
     2041 gacggaatac cgaactggcg ctggccttcg ccatcgccgc ggacgggatg gagggagtta
     2101 cgttcctgtc ggcagggacg gacgggacgg acggaccgac ggacgcagcc ggggcgatag
     2161 tggacggggg gacggtgaag agggggagag agaaggggct cgatgcgcgg gagtacctgg
     2221 aaaggaatga ttcgtacagc ttcttcaagg aaacggggga gctattgata acggggccga
     2281 cggggacgaa tgtgatggac ctggagattg tcctcataga aaggccatga ggacgcttga
     2341 gccgctcgaa ccgcttgaac gaagaaaaga tggcttgaat ggcagccgct tgagcgttaa
     2401 cagatagaaa gacttgcgga cttgtctcgc acttgttatt tgcttcggct caccatgtat
     2461 gctacggcgt tgccgacctg ggcatccgtc agcttggggt ttccgccctt cttcggcatc
     2521 atgcctttct tgccgcgaaa accgtttatg gcccttgtca ccattacatc catgccctgc
     2581 ttgatacgag cggcccaggc ggctttatcg cccacttttg gcgctcccat catgcccgct
     2641 ttatggcaca tggcacaggt tgcatcgtac gtggcctttc cggccagttt atcgtacctg
     2701 gcggatgagg cattggtcgc tcccatgcat acgatcgata ctgcaaacag cgcgatggaa
     2761 acaaaacgga acatggcatg accccctttc agtttgcatc gatttaaaac actatagaat
     2821 tatcaattcc gtatgtcaaa cgatacagaa atatgccaga ctgaatgccg gggggtcgtt
     2881 agaaagaaaa ggttttatgt ctcgcccgct ttgctcgagt ccgcggaggg cgtggagaaa
     2941 tgaataaaag atcgcggccg gatttcggag cccaccaaga tccggccgcc tttgcatccc
     3001 gtctgtgacg ggaggcaaaa catagggttt tctctgtgtc ctccgcggac ttgagcgacg
     3061 cgggcgagag gcaagtcttt gatattcttt atcccgtcgg acgtgtttgt gaggaaagtg
     3121 aacgctggag gggagtgaat gattggggct gttgtcctct ccgcgacgcg ccggtatcct
     3181 gtgaaaggac gtcaaatgcg agtgatggga aggaaatccc ttcggcccca cgtgatggac
     3241 gatcatgcaa gctttttcag cgatgccttg ataaaagccg ggaggtccgc ggggcttctg
     3301 cttgatatca ggttgccatc ctccacgagt tcacggtcct cgaaatcagc gcccgcgttg
     3361 atgatgtcct ggacgatcga tttccagccc gtgatctttt ttcccttaag gacgttcgcc
     3421 gtgataagca gctgtggtcc gtggcagatc acgaatacgg gcctgccgct ctccacgaat
     3481 tccttcgcga accggaccgc gtctgcgtcc accctcagct tgtcgggaga gtaaccgccg
     3541 ggaataagaa gcgcgtcaaa ctcgtctgca tacacgtcct tcaccgcctt ttcgaccttc
     3601 accgccgtcc cggacgcctt ccccgtgacg gtcttgccgg ccgaaagccc gatatgcacg
     3661 agcgcatgtc ccgcctccct gaatgccttc gccggctcgg tatactccga atcctcgaac
     3721 atatccgcga tgatcaccgc gatcttcgcc attgtcctcg cctcctgtct ttcgtttctt
     3781 tccaacctca gctaaacgta agcacagcgg tggtacccgt caaccatccc gggggatccg
     3841 gggaggacct acagtatgtt gtgccgagcc tgtcaagctc gccaataaac tccctgtcca
     3901 atgtgacggc catcttttcc ttgcccatct ctcctccaca atagtatgac atatatcata
     3961 ccagcgatgc ctgtcacgcc ggggcaactg accctctcag attggggaac tgaccctgga
     4021 agtgatacaa taaatgtgag ccggtcatga agaatgcgac ccgatttata tgtggctgaa
     4081 aatgtgaccg gcccctggat atgctgatga aagcaggagg aaaaagggat tggatattca
     4141 tcggcgccgt catcgtcctt gtcacggcgc tgcacttcct tgtgcttgca agacattcac
     4201 cgctcgttgt ccttgaggaa ctgtattatg taccgctgct cctcggagct ttgcgcttcg
     4261 gtttgaaagg agcccttgtg gtgtatgtcc ttgtttccgt gcttcatatc cctttctttt
     4321 ttggtgaatg gacggcttct ccgataggcg tggcagacag ggtgcttcat ttcgtttttt
     4381 cggggatatt tgccttcacg gccgggttcc tcgttgatcg cgagcggaga aagagagagc
     4441 aggcggaaag agaaaggtac cttgccgggg tcggaatggc ggccacaacg atcgtgcacg
     4501 acctgaggaa ccagcttcat cttattctcg ggtacgggtt gcgcatccag aagggcaagg
     4561 gagacatcga
//
LOCUS       DAOPOA010000094         4555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_20639_length_4555_cov_2.986889, whole genome shotgun sequence.
ACCESSION   DAOPOA010000094 DAOPOA010000000
VERSION     DAOPOA010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20639_length_4555_cov_2.986889"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1766)
                     /locus_tag="PKJ17_03060"
     CDS             complement(<1..1766)
                     /locus_tag="PKJ17_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOD74979.1"
                     /translation="MRYPAIMIVPFILGCLGPMAFASEDAITTRPLNEDPPPVTRAIQ
                     LETSPQAVRSVPSDASAAPQATPTAQQAAPAAQTTTPARVQAVPAAPQTTRSAPEANE
                     APASPSKPPAPSPPPGPGAAKATPTAQQTAPAVQPTPPRVGQPPRPQQPPSFQPPRPQ
                     QPQRVQPARPPAAGRISLNFDDADVFSVIQTVFGEILKVNYVVDQRVKGRVTFRSVAP
                     VTLDQVLPIMETILRINGVGVVEESGLYRVVPISDVAKEPAQVKFGREPEKVLIQGKS
                     IIQVVPLLHVHSSETIKFITPFLTATAVVIDVPNINYIILVDTDSNVKRLLTLISFFD
                     GEQTKQKKPKVSVYNIQNGKAKDVAAILQQVLLSAKPPQAPTATATSAPATGSATPGT
                     PPPAQPQAGRPSLQAAGTQGGDTLVSPITKIIADEGLNALIILSTQEDYEFIRAAIER
                     IDVIPRQVLLEGVVAEVSLKDELKLGISWALQFNPGGMRGVLGGVEGTVGFNVPGATP
                     TSTTGAGTFTFAGTVGGDFKTVIDMLATDSRAKLLAVPHVLVSDNKEARIQVGQQVPV
                     VSSETFGSGTVAPQRTIQYKDIG"
     gene            complement(1800..2225)
                     /gene="gspG"
                     /locus_tag="PKJ17_03065"
     CDS             complement(1800..2225)
                     /gene="gspG"
                     /locus_tag="PKJ17_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOD74980.1"
                     /translation="MRKILNRQNRKGFTLIELLVVMVIVGLLAALVAPRLFPKLGKGK
                     QAAAKAQIELLGQALDQMRLDVGRYPTTQEGLNMLAVNPGVEGWDGPYLKKALPNDPW
                     GKPYNYQSPGSHGEYDLISYGRDGSPGGEGEDADVTSWQ"
     gene            complement(2597..3970)
                     /locus_tag="PKJ17_03070"
     CDS             complement(2597..3970)
                     /locus_tag="PKJ17_03070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD74981.1"
                     /translation="MSAGKILVVDDERNLLELIQMGLEQAGYDVVTATDEVEALIQAR
                     EQVIDLAVLDLQLVRTDGITLMEQLHLISPRMPVIILTGYGSIESAVEAMKRGAYGYL
                     TKPFDSQVLLFEIGKMMENRRMSSEIERLQGLLESKYSFSNIIANSKKMQDVLETISR
                     IAKTDSTVLLLGESGTGKDLIAKAIHLASDRKEKSFVAINCAALPEPLLESNLFGHEK
                     GAFSGAIRETKGLFPQAHGGTIFLDEIGDMPLSIQAKILRVIQERKIRPVGSEKVIDV
                     DVRIVTATNKNLEEQVRLDLFREDLYYRIHVIPIQLPPLRERKEDIPPLVDFFLQKAG
                     ALMRKDIKGVTPEAMQKLMLYDWPGNIRELENVIEYAAAMTDREYITEDLVFQTKGVV
                     SQEPLRPLREAKEAFEKSYLIHLLEICKGNITEVAKCAGKYRADLYDLLRKHNLRPED
                     FKKRESA"
     gene            complement(3980..4429)
                     /locus_tag="PKJ17_03075"
     CDS             complement(3980..4429)
                     /locus_tag="PKJ17_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74982.1"
                     /translation="MPERAAEEPDRTSQAFSDARRDLDEAQILLARGNYQLSLMVLER
                     VLAGPAGRAHGDEVLFTMGLIFAHPGNQNRDYQKSAAFLRRLVKEYPSSSLRDQARTW
                     AEVLEENIKLRRASSEALQENQKLKQIIEQSKKVDIEIEEKKREEKR"
BASE COUNT          793 a         1379 c         1349 g         1034 t
ORIGIN      
        1 ccgatgtcct tgtactggat cgtccgctgg ggcgccaccg ttcccgaacc gaaggtctcc
       61 gatgagacga cgggcacctg ctgacccacc tggatacggg cctccttgtt atccgacacc
      121 aggacatggg ggacggcaag cagcttcgcg cgcgaatccg tggcgagcat gtcgatgacg
      181 gtcttgaaat ccccccccac ggtgcccgcg aaggtgaacg taccggcgcc ggtggtgctc
      241 gtcggcgtcg caccgggcac gttgaacccc accgtgccct caaccccgcc caggacgcct
      301 ctcatgccgc cggggttgaa ctggagcgcc caggatatgc ccagtttcag ttcatccttc
      361 aggctcacct ccgccaccac gccctcgaga agcacctgcc gcggtatcac gtcgatcctc
      421 tcgatcgccg cccggatgaa ctcgtagtct tcctgcgtgg acaggatgat gagagcgttg
      481 agcccttcgt cggcgatgat cttcgtgatc ggcgatacga gcgtatctcc cccctgcgtg
      541 ccggcggcct gcaatgaagg acgtcccgcc tgcggttggg ccggcggagg cgtgccgggg
      601 gtggccgaac ccgtcgccgg ggctgaagtg gccgtagcgg tgggggcctg aggaggtttc
      661 gcggataaaa ggacctgctg caggatggcc gccacatcct tcgccttacc gttctgaata
      721 ttgtacacgg agactttcgg ctttttctgc ttcgtctgct cgccatcgaa gaagctgata
      781 agcgtgagga gccgcttcac gttcgaatca gtgtccacca ggatgatgta attgatgttg
      841 ggtacgtcta tcaccacggc tgtcgccgtg agaaaggggg tgatgaattt gatggtctcc
      901 gacgagtgca cgtggagcaa cggcaccacc tgtatgatgg acttcccctg gatgagcact
      961 ttctccggtt ctcgtccgaa cttaacctgg gccggctcct tcgcgacatc gctgatgggc
     1021 accacccggt acaggccgct ctcctcaaca acaccgacgc cgttgatacg caggatcgtc
     1081 tccatgatgg gaaggacctg gtcaagggtt acgggggcaa cggagcggaa ggtcaccctc
     1141 cccttcaccc tctggtcgac aacataattc actttcagta tctctccaaa aacggtctgt
     1201 atcaccgaga aaacatcggc gtcgtcgaag ttgaggctga tccggcccgc cgcgggagga
     1261 cgcgcgggct gaaccctctg gggctgctgc ggccggggtg gctgaaaaga agggggctgc
     1321 tgcggccggg gtggctggcc caccctgggg ggggtcggtt gcacggcggg tgcggtctgt
     1381 tgcgcggtgg gggtggcctt cgccgccccg ggccccgggg gtggagacgg ggccgggggt
     1441 tttgaaggtg aagccggcgc ctcattggcc tcgggcgccg atcgggtggt ctgtggcgcg
     1501 gcggggacag cctgtacccg cgcgggggtc gttgtttgcg ccgcaggcgc agcctgctgt
     1561 gccgtcgggg tggcctgtgg cgcggcggat gcgtcagacg ggaccgatcg caccgcctgc
     1621 ggggatgtct cgagctggat agcccgcgtg accggaggcg ggtcctcgtt cagggggcgt
     1681 gtcgtgatgg cgtcctcgga ggcgaacgcc atcggtccga gacaccccag aatgaaagga
     1741 acgatcatta tcgccggata tctcatggtc cgggccttgt ccttgtgctc atatcacggc
     1801 tactgccagc tcgtcacgtc cgcgtcttcg ccttctccgc cgggcgaacc atccctcccg
     1861 taggagatga ggtcatactc cccgtggctc cccggggact gatagttgta tggtttaccc
     1921 caggggtcat tgggcagggc ctttttaaga tagggcccgt cccacccctc cactccgggg
     1981 tttacggcaa gcatgttgag accttcctgc gtcgttggat atcgtccgac atcaagcctc
     2041 atctggtcca gggcctgccc gagaagctct atctgggcct tcgcggcggc ctgttttcct
     2101 ttccccagct tgggaaatag cctcggcgcc accaaagccg cgagcagacc cacaatgacc
     2161 atgacgacaa gaagctcgat gagcgtgaaa cctttcctgt tctgcctgtt aagaatcttc
     2221 ctcatctggg acccccggta tggcctcccg ggcgaccaca ggtccgtccc ggaaaagagt
     2281 tcctgacgtg ccagcctata tctgcaggat acaaaatagc cctccttctg tatgtcataa
     2341 aaacgacaat tatcttgtag tgcatccacc atctcctgtg tcatcgtttt cgattacaat
     2401 acccgatgag agggccgcgg aggagcacga agaaggatag gacctgtagg agagatagga
     2461 catataggac ttttcaggcc ggagttggcc gaccggcacg tcctattggt cctatttcgt
     2521 cctattggtc ttatatgtcc tattggtcct atctctctta caggtcctat catctctttc
     2581 ccctctgcct tttcttctac gccgactccc tcttcttgaa atcctccggt ctcaggttgt
     2641 gcttcctgag gaggtcgtag aggtcggccc tgtacttgcc cgcgcacttc gccacttcgg
     2701 ttatgttgcc cttgcatatt tcgagaaggt ggatgagata gctcttctcg aaggcctcct
     2761 tggcctccct gagaggtctg agcggttcct gggaaacgac gccttttgtc tgaaagacga
     2821 ggtcctccgt gatatactcc ctgtcggtca tggcggccgc gtactcgatc acgttctcca
     2881 gttcccggat gtttcccggc cagtcgtaga gcatcagctt ttgcatggcc tcgggggtca
     2941 cgcccttgat atccttcctc atgagcgcac cggccttttg gagaaagaag tcgacgagag
     3001 ggggaatgtc ctctttcctt tcccgtaacg ggggcagctg gatggggatc acgtggatcc
     3061 tgtagtaaag gtcttcccgg aagagatcga gccgcacctg ttcctcgagg ttcttgttcg
     3121 ttgccgttac gatcctgaca tcgacgtcga tgaccttttc gctgccaacg ggacggatct
     3181 tcctctcctg gatgacgcgc agtatcttag cctgaatgga gagaggcatg tcgccgatct
     3241 catcgaggaa gatggtcccg ccatgggcct gcggaaagag ccccttcgtt tctcggatgg
     3301 ccccgctgaa ggcccccttt tcatgcccga agaggttgct ctccaggagg ggttcgggaa
     3361 gggcggcgca gttgatggcc acgaaagact tttccttcct gtcgctggcg agatggatcg
     3421 ccttcgcgat gaggtccttt ccggtgccgc tctcccccag gagcaggacg gtggagtccg
     3481 tcttcgcgat gcgcgatatc gtctcgagca cgtcctgcat cttcttgctg ttggcgatga
     3541 tgttggagaa gctgtatttc gattccagaa ggccctggag cctttcgatc tccgaggaca
     3601 tccgccggtt ttccatcatc ttccctatct cgaagagaag gacctgggaa tcgaatggtt
     3661 tggtgagata tccgtaagcc ccgcgtttca tcgcctccac ggcgctctcg atgctcccgt
     3721 aaccggtgag gatgatgacg ggcatccggg ggcttatgag atggagttgc tccatcagcg
     3781 ttatgccgtc ggtacgcacg agttgaaggt cgagaacggc gagatcgatc acctgctctc
     3841 tcgcctgtat cagagcctcc acctcatccg tcgcggtcac cacgtcgtag cccgcctgtt
     3901 caaggcccat ttggatgagt tccagaaggt tcctctcgtc atcgactacc agtatcttac
     3961 ccgcggacat aggtctctcc tatcttttct cttccctctt cttctcctcg atctcgatat
     4021 ccaccttctt cgactgctct atgatctgct tcagtttttg gttctcctga agggcctccg
     4081 aggacgcacg tctgagcttt atgttctcct caagcacctc cgcccaggtc cttgcctggt
     4141 ccctcaggga actggagggg tattccttga cgagccgcct gaggaaggcg gcggacttct
     4201 gatagtccct gttctggttc cctggatggg cgaagatgag gcccatggtg aagagcactt
     4261 cgtctccatg ggcacggccc gccggcccgg ccaggaccct ctccaggacc atcagggaca
     4321 actggtagtt gccccgcgcg aggagtatct gtgcctcatc gaggtcccga cgcgcgtcgg
     4381 agaacgcctg agaggtcctg tcgggctcct cggccgccct ctccggcacc gcgcaaccca
     4441 aggtcgcgag aaacatcaca agggcaatac aaagagaaac gtggttccct gtccgggttc
     4501 gctttccgcc cacaccttac ctccatgcgc tgtgatgata tgtttcacga aagcc
//
LOCUS       DAOPOA010000095         4550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_20665_length_4550_cov_3.768409, whole genome shotgun sequence.
ACCESSION   DAOPOA010000095 DAOPOA010000000
VERSION     DAOPOA010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20665_length_4550_cov_3.768409"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PKJ17_03080"
     CDS             complement(<1..269)
                     /locus_tag="PKJ17_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74983.1"
                     /translation="MFSGSGERGGRGPGAILSGIADIVQLAEDYLNEAVDASHGYDPQ
                     VVSQIRADLASLDAFLARLTEVRTMRDDTLFRKAAVELRSQVPAL"
     gene            complement(715..1434)
                     /locus_tag="PKJ17_03085"
     CDS             complement(715..1434)
                     /locus_tag="PKJ17_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOD74984.1"
                     /translation="MDIYERLKDELRLLAGDSMDEKVSVVSARALTAKEAIGETGRDD
                     YPLLKGKEAMVEALYKGVKGHAFTDMPGGFEGTVRDVIMLPLANNFERAVFIATINAV
                     MREAGLITGTVHCRDDEPGRCARELVSYVREKFGNPRIAFVGFQPGMIERLSEVFQMR
                     VLDLDEDNIGKKKFGIVIESPEATEDVLAWGDIILATGSTCVNGTITRFVGQKPVIFF
                     GVSGAAVTKLMGFERYCRYPH"
     gene            complement(1620..2480)
                     /gene="sppA"
                     /locus_tag="PKJ17_03090"
     CDS             complement(1620..2480)
                     /gene="sppA"
                     /locus_tag="PKJ17_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOD74985.1"
                     /translation="MAGRTGRKVLLIVTVVIAAVIFLASILPGIIGGAGGGKIGVVEV
                     EGTIADLKDVMADIVRFKEDKGILGVVVRINSPGGAVGPTQEAASELKKLKAVKKVYV
                     SMGSVCASGGYYIAAVGEKLYANPSTITGSIGVIMQQTVVEELMKKIGVQSNTIRAGA
                     MKDVGSPFRKMTDNERQYLQDIINNIHEQFIKDVAAGRKMPLDKVRSLSDGRIYTGLQ
                     AKEAGLIDGIGTFYDVVDDMKKAVGITGKPELVYGKRPFAPLKWLFSSMAREMLGREQ
                     ADPFQYKYAP"
     gene            complement(2550..3257)
                     /locus_tag="PKJ17_03095"
     CDS             complement(2550..3257)
                     /locus_tag="PKJ17_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOD74986.1"
                     /translation="MSLLVPALEVQLECYEGPMAVLITLIKRNKVSIWDIPIALITDR
                     FLQYVEIAKEMNLKIAEDFIEMASLLLFIKSKMLLPSNGATDEEDPREELVERIIEYE
                     RVRNMAVAIDDLPMLERDVYCIGRGSLEKETDFDLLCLCNLYFELMNVKEEPYFMVRD
                     VRPTLEEKIAALKDLLRSDGFFEWDIAAGEERNERVATILGMLELAKIRVATLTQRRP
                     FGRIIMKTRDAAALAEG"
     gene            complement(3254..4489)
                     /locus_tag="PKJ17_03100"
     CDS             complement(3254..4489)
                     /locus_tag="PKJ17_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74987.1"
                     /translation="MGNLADEMGFHAYLVGGFVRDLLLRIENYDIDIVIEGDGIAFAE
                     EMVRKFHTRMRSHREFATAKVLFPDGFKIDIATARLEYYRAPAALPTVEHGSLKLDLH
                     RRDFTINTLAISLNRNTFGELVDFFSAQRDIKEKTIRVLHSLSFVEDPTRVFRAIRFE
                     KRFGFKIGKQTLNLIKNAVKLNFLARMRGKRIWTELALILREDAPEAILARLAELDLL
                     RFISPDIVFNKDKEKLFAQMHGVYQWHEFLYQGKTVDKVQFYILAIVDNMKLSDIAEF
                     AARMEISERFRRHTMENVERLRLAMARFSQGVAGIKKSEVYRLLDGLSREALLFIMAR
                     TRSQEVKKAISNYITHHDSFRPFTTGNDLKRLGVPEGPLYKEILEDLKDAKIDMDLKT
                     KEAESHYLETYLGERGILT"
BASE COUNT          900 a         1389 c         1259 g         1002 t
ORIGIN      
        1 tggagcgccg ggacctgcga tctcaactcc accgcggcct tcctgaaaag ggtatcgtca
       61 cgcatcgtcc gcacctcggt caggcgcgcc aggaaggcgt cgagggacgc gaggtcggcg
      121 cggatctggc tgacgacctg aggatcatac ccatgactgg catcgacggc ctcgttgagg
      181 taatcctccg ccagctgaac gatatcggcg atccccgaca ggatcgcccc gggaccacgg
      241 ccgccccttt cgcctgatcc agagaacatg gcattcctcc tcttcagtga tcgtttccgc
      301 ggtacccgcg ctatactaaa atattaaccc tccgacctcc tcattgcaag gagcggaggt
      361 tgccggagtc gatgcggggg attgaaaaag actgatcgac ggccgtcaag ggtccgcgac
      421 ggccgtgccc gtcgcgccat catacacggg cacatcgata ttcaccagaa ggcctccgtc
      481 cacccgcgtt ccggacggtc acgcttcaga aagaaaacgc ctccagtgca catcccggaa
      541 gcgttgtctc aaggtatgcc gtcctcttcg aacaacatgg gacgaatgac atatcgctcc
      601 acttcgacgg aacggcggta acggtgctcg cgaacgggaa agacctcctg acaggcctct
      661 cgtgaagccc ggggtcgtcg ccgctcagct atggttccgg cggccctgcg ccgctcaatg
      721 aggataccgg caataacgtt caaaacccat cagcttcgtg acagccgctc cggagacccc
      781 gaagaaaatg acgggctttt gaccgacgaa ccgggtgatc gtcccgttga cacaggtcga
      841 acccgttgcc agaatgatgt cgccccaggc aaggacatct tcagtggcct cgggactctc
      901 gatgacgatg ccgaacttct tcttcccgat gttatcttcg tcgaggtcga ggacccgcat
      961 ctggaagacc tcggagagcc gctcgatcat gccgggctgg aagccgacga aggcgatgcg
     1021 ggggttcccg aacttctctc tcacgtagga aacaagctcc cgggcacatc gtcccggttc
     1081 gtcgtcccgg cagtggacgg tccccgttat cagtccggcc tcgcgcatga ccgcgttgat
     1141 ggtggcgata aagacggccc gctcgaagtt gttcgcgaga ggcagcatta tgacgtcgcg
     1201 cacggtcccc tcgaaaccgc ccggcatatc ggtaaaggcg tgtcccttta cacccttgta
     1261 aagagcctcc accatcgcct ccttgccctt cagaagcgga taatcatcgc gccccgtttc
     1321 acctatcgcc tctttcgcgg tcaacgcccg ggccgaaacc acgctcacct tttcgtccat
     1381 cgagtcaccg gccaggagcc tgagttcgtc cttaagcctt tcataaatgt ccaccaaaga
     1441 gtcaccccct ttacagttcg ttgtatagct tgttagctag cgtaatatag ccggattgga
     1501 ggtggattgt caacggggag aagagaaaga ccgctgcaac cgcttgaacg ttaagagaca
     1561 acatccaacc gtctcgaacg tttaagcggc tcaagcggtt taagcggttg cacccgtctt
     1621 cagggtgcgt acttgtactg gaaagggtcc gcctgttcgc ggccgagcat ttcgcgcgcc
     1681 atggaggaga agagccactt gaggggcgcg aaaggccttt tcccgtagac gagttccggt
     1741 ttgcccgtaa tgccgacggc cttcttcata tcatcgacga cgtcgtagaa ggtgcctatt
     1801 ccatctatga gacccgcttc cttcgcctgg aggcccgtgt atatcctgcc gtcggagagg
     1861 ctccgcacct tgtcgagggg cattttccgt ccggcggcca cgtccttgat gaactgctcg
     1921 tgaatgttgt tgataatatc ctggaggtac tggcgctcgt tgtctgtcat cttccggaag
     1981 gggcttccca cgtccttcat cgcccccgcc ctgatcgtat tgctctgcac tcctatcttc
     2041 ttcatcagtt cttccacgac ggtctgctgc atgatgacac cgatggaacc ggtgatggtg
     2101 gacgggttgg cgtacaactt ttcgccgaca gcggcgatgt agtaacctcc tgaagcgcag
     2161 acggacccca tcgagacgta gaccttcttg accgccttga gtttcttgag ttcgctcgcc
     2221 gcttcctgtg tggggcccac ggccccgccg ggcgagttga tccgcaccac gacaccgagg
     2281 atacctttat cctccttgaa cctgactata tcggccatga cgtccttaag gtcagcgatc
     2341 gtgccttcga cctctacgac accgatcttt cctccgccgg ctccgccgat gatgccgggc
     2401 agaatactcg cgagaaagat cacagccgct atcacgacgg taacaatgag caacaccttc
     2461 ctgcctgtgc gaccggccat ttcattcctc cttgcagcgc ccgtgccaga aaaccgctcc
     2521 cgctgctcct gtcagatcgg caccgcgact catccttcgg ccagcgccgc ggcgtcccgt
     2581 gtcttcatga taattctacc aaaaggtcgc cgttgtgtca gcgtcgccac ccgtatcttg
     2641 gccagctcca gcatcccgag gatggtggcc acgcgctcgt tcctttcctc cccggcggcg
     2701 atgtcccatt caaagaaccc atcggacctc agaaggtcct tgagcgccgc gatcttctcc
     2761 tccagcgtcg gtctcacatc acgcaccatg aaatagggtt cctctttgac gttcatgagc
     2821 tcgaagtaga gattgcacag gcacagaaga tcgaaatcgg tctccttttc caggctgccc
     2881 cgcccgatgc aatagacgtc gcgttccagc atgggcaagt cgtcgattgc gacagccatg
     2941 ttcctgacgc gttcatactc gatgatgcgc tccacgagtt cttcgcgggg gtcctcctcg
     3001 tccgtcgccc cgttggaagg aaggagcatc ttcgacttga tgaagagaag gagggaggcc
     3061 atctctatga agtcctcggc tatcttcagg ttcatctcct tcgctatctc gacgtactgg
     3121 aggaagcggt ccgtaatgag ggcaatgggg atgtcccaga tgctcacctt gttccgcttg
     3181 ataagagtga tgaggaccgc catgggcccc tcatagcatt caagctggac ttccagcgcg
     3241 ggaacgagaa gactcatgtg aggatccccc tttcgccgag ataggtctca aggtagtggg
     3301 actccgcttc cttggtcttg aggtccatgt cgatcttggc atccttgagg tcttcgagga
     3361 tctccttgta aagaggtcct tcaggcacac cgagcctctt gaggtcgttg ccggtcgtga
     3421 agggcctgaa cgagtcgtga tgggtgatgt agttcgagat cgccttcttt acctcctggg
     3481 aacgggtcct ggccatgatg aaaagcaggg cctcccggga aagtccatcc agcaggcgat
     3541 agacctcgct cttttttatg cccgccactc cctgggaaaa acgcgccatg gccagccgca
     3601 ggcgttccac gttctccatg gtgtgcctcc ggaaacgctc gcttatctcc atgcgcgccg
     3661 cgaactccgc gatgtcggag agcttcatgt tgtccacaat ggcaaggatg tagaactgga
     3721 ccttgtcgac tgtcttcccc tggtagagga actcgtgcca ttgatatacg ccgtgcatct
     3781 gcgcgaagag cttctccttg tccttgttga agacgatgtc cggcgagatg aaacgcagaa
     3841 ggtcgagttc cgcgagcctg gcgaggatgg cctcgggggc atcctccctc aggataaggg
     3901 ccagttccgt ccatattctc tttccacgca tcctagcgag aaaattcagc tttacggcat
     3961 tcttgatgag gttcagggtc tgcttgccga tcttgaaccc gaatcgcttc tcaaagcgga
     4021 tggcgcggaa cactctcgta gggtcctcca cgaagctcag gctgtggagg acacggatag
     4081 tcttttcctt gatgtcccgc tgggcactga agaagtccac gagttcgccg aaggtgttcc
     4141 tgttaaggga tatggcaagg gtgttgatcg tgaaatcgcg gcggtgcagg tccagtttca
     4201 gcgagccgtg ctcgacggtg ggcagcgcgg ccggtgccct gtagtactcg aggcgggccg
     4261 tcgcgatgtc gatcttgaac ccgtcgggaa agagcacctt tgccgtcgcg aactccctgt
     4321 gggaacgcat cctggtgtgg aacttcctga ccatctcctc ggcgaaggcg atgccgtcac
     4381 cctcaatgac gatatcgatg tcgtagttct cgatgcgaag aaggagatcg cggacgaaac
     4441 cgcccacgag gtaggcatga aaacccatct cgtcggcaag gttgcccatg tccacgagct
     4501 tcccattgtc gcctcatcca gtcgctcctc catgagcttg cggacgttct
//
LOCUS       DAOPOA010000096         4533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_20770_length_4533_cov_3.754578, whole genome shotgun sequence.
ACCESSION   DAOPOA010000096 DAOPOA010000000
VERSION     DAOPOA010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20770_length_4533_cov_3.754578"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKJ17_03105"
     CDS             complement(<1..242)
                     /locus_tag="PKJ17_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74988.1"
                     /translation="MNGNKLYVGNLSYSVTGERLRELFSGYGEVGEVRVIEGKGFGFV
                     EMATKEGAEKAKQALNGMDFEGRTLRIDDARPQKERP"
     gene            517..1248
                     /locus_tag="PKJ17_03110"
     CDS             517..1248
                     /locus_tag="PKJ17_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HOD74989.1"
                     /translation="MRKAVSRLSICFATIFCIFLFAPFGAFPLGPAPASCDDAVQARQ
                     LVDKARMTLESFAEAREMEGFRNLMKDAEGIFVSPQVLRGAFIFGASGGSGVFMVRDK
                     TKRDWAGPAFYTIGEASFGLQIGGDASEVVLLAMTQRGVMALLSSSVKLGADVGIAVG
                     PVGAGADAATANLSADIISFSRSKGLYGGVSLEGAVVAVRDGLNGAYYGRNSSPSDIL
                     IARSVKNDHAAGLRSMVTKIAGGKN"
     gene            1306..2097
                     /locus_tag="PKJ17_03115"
     CDS             1306..2097
                     /locus_tag="PKJ17_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOD74990.1"
                     /translation="MRNRSRIAIFTALTGLILLVSFPVHAGVDPAYQDGLNRLLGDLE
                     KQGFTSEELTGIFSDSRFELYPHIVGRTGKGMNYMSRRFGLLTKRSIKRGRNILATYR
                     DVLKNAEKDYGVDKEIIVAILRVETNFGSVTGTYPVLNSLATMAVIENRRSEWARGEL
                     VELLALCREQNRDPFSIRGSWAGAFGIAQFIPSSYVKFAVDGNSDGRIDLFTMSDAVA
                     SVANYLKSHGWEKGKPEGNRQAVYAYNHCNSYVKAVFAYAKALRR"
     gene            complement(2154..2960)
                     /locus_tag="PKJ17_03120"
     CDS             complement(2154..2960)
                     /locus_tag="PKJ17_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74991.1"
                     /translation="MRRTLLLATGGMIGFAAMVYTSAAAAQGCPPNSAPVSEKDNVVH
                     CRCIPGFENRGGVCVRVAEPAGGTKPEDVRFPPAAANLVNIMDKVTKELNRPKGGYHK
                     DLEPRLVCNLFFQGVGKELNRLGLPAGTGAWKKGLKAAQIKARIEADKSAWSEVQEED
                     VQQRANRGIIVVAVSNDHVAIAFPVPPDTQFSAKGPLFRDGNEHGPERQADRRLHASS
                     WGAIPARNMFGYEYSDKEKKQLKSPRFYVWVPSESPASTQSAYKRKVAPR"
     gene            complement(2961..3776)
                     /locus_tag="PKJ17_03125"
     CDS             complement(2961..3776)
                     /locus_tag="PKJ17_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74992.1"
                     /translation="MSKRSVHGQGERCGWGTAAVPGIAVLLILVLAAALPPDAHAGNG
                     GQAVSAQCWINGKLVGGFPLNKPCPGTEGGGGRAVIMGPPVIVWRRPSAEGSQMAGLA
                     PVDSKLAGQLGAIADTPITLRQKGEFAMRNGDLAQAKLYLEEANRRDPQDPQIQQSLA
                     KLRGLFDSGTQSLKVSRLKLPEQEFLKPMPAATVDRFMGNREVRALRQAEIISYNRLA
                     NADAALNAAKADAQAGKTTQKDVDQAQLKLNAAVEEFKKAETKLKEKIYVLDK"
     gene            complement(3791..4222)
                     /locus_tag="PKJ17_03130"
     CDS             complement(3791..4222)
                     /locus_tag="PKJ17_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74993.1"
                     /translation="MLGSLRALGVIALIACALLFTAGQTFAGPLDSIPVGPSDRVLLN
                     QNVNLNENGSASNLFVPQQATVFFDVAVQPGKKLLLMVITEDQWKAISSGEKPSGSPI
                     LRTHVSGVDSVSVSIPRGTYTVAMIPAQGATQVVMRARARY"
     gene            complement(4230..>4533)
                     /locus_tag="PKJ17_03135"
     CDS             complement(4230..>4533)
                     /locus_tag="PKJ17_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD74994.1"
                     /translation="VTVSANGAVGSDIHVSSRAFLSIDFAVQPGRQVTLLVITGAQKR
                     EMDAGKQITGRPQVRAPIDGAASQSLTVNQGGYYVAILNSNPQAVSVTYRVCARAF"
BASE COUNT          860 a         1323 c         1348 g         1002 t
ORIGIN      
        1 ggtctttctt tctgcggccg ggcatcgtcg atcctcaatg tcctgccctc gaaatccatt
       61 ccgttcagag cctgttttgc cttttccgcg ccttccttcg ttgccatttc aacgaatccg
      121 aaaccctttc cttcaatgac ccgcacttcc cccacttcgc catacccgga gaacaactcg
      181 cgcagcctct cacctgtcac ggaatagctc aggttgccta catacagctt gttgccgttc
      241 atggccgata cctccctttg gattggactg tatttcatat ttcaagcgag atagcgtgtc
      301 ccgaacggac tatcccgtga aacatgggag gagaaagaga caaataccag gtagcagaga
      361 ttgacccgtt cccttccggg aatccgatgt ataagccaat cataaaacgc tttacccggc
      421 attgacaagg aaaaagtatt cctttaccat tttacgcaaa ggcggtcggg cgcggctgaa
      481 gggactgtcg cgggaagacg ccggggagga tgttgcatga gaaaagcggt ctctcgtttg
      541 tcgatctgtt tcgccacgat cttctgtatc tttctgttcg cccccttcgg tgcgttccct
      601 ttggggcccg cgcccgcgtc ctgcgatgac gccgtgcagg cccggcagct tgtcgacaag
      661 gcccggatga cactcgagtc tttcgccgaa gcgagggaga tggagggatt tcggaatctc
      721 atgaaggatg ccgaggggat cttcgtctct ccccaggtgc tcaggggggc tttcattttc
      781 ggtgcttccg gcggcagcgg cgtcttcatg gtccgcgaca agaccaaaag agactgggcg
      841 ggaccggctt tctacaccat cggcgaggca agtttcggtc tccagatagg cggtgacgcg
      901 tcggaggtgg tccttctcgc gatgacgcaa cgaggggtga tggcgcttct ttcgagcagt
      961 gtaaagcttg gcgcggacgt gggcatagcc gtcgggcctg tcggcgcggg tgccgatgcg
     1021 gcgacggcaa acctaagcgc agacatcatc agtttttccc ggtccaaggg tctctacggg
     1081 ggcgtctccc tcgaaggcgc cgtcgtggcg gtgagggatg ggttgaacgg ggcctattac
     1141 ggaaggaaca gcagtccctc ggacatcctt atcgcgaggt cggtcaagaa cgatcacgcc
     1201 gcgggcttga ggtcgatggt cacgaagatc gcgggaggga agaattaagg cacgccgccc
     1261 gttctgtacc atctccctgg tcccgctttt ttgcaggggt atataatgag aaacaggtca
     1321 cggatcgcga tattcaccgc tctgacgggt ttgattctcc ttgtttcctt tccggttcac
     1381 gccggggttg atcctgccta tcaggacggc ctcaacaggc tccttggcga tcttgagaaa
     1441 caggggttta ccagcgagga gctcacgggc atattctcgg acagccgctt cgaactgtat
     1501 ccgcacatag tcggtcgaac gggaaaaggc atgaactata tgagccgcag gttcggcctt
     1561 ttgacgaagc ggtcgataaa acggggcagg aacatactgg caacgtaccg cgacgttctg
     1621 aagaacgccg agaaagatta cggtgtggac aaggagatca tagtggcgat ccttcgggtg
     1681 gaaacgaatt tcggttccgt gacgggcacc tatccggtcc tcaacagtct cgcgacgatg
     1741 gccgtcatcg agaaccggcg ctccgagtgg gcgcgcgggg aactcgtcga actccttgcc
     1801 ctgtgcaggg aacagaacag ggaccctttc tcgatacgcg gctcctgggc cggtgccttc
     1861 ggcatcgcac agttcatccc gtcttcctac gtgaagtttg ccgtggacgg caactccgac
     1921 ggcaggatcg acctcttcac gatgagtgac gccgtcgcca gcgttgccaa ctacctcaaa
     1981 agccacggct gggaaaaggg caaacccgaa gggaatcgtc aggccgtcta cgcctacaat
     2041 cactgcaaca gttatgtgaa ggccgtgttc gcctacgcga aggcattgcg cagataaaga
     2101 ctgttcaagg gttcaaaggg tgtaaggcag gaggcgaaag tgccggggca tgttcacctg
     2161 ggtgccacct tgcgtttgta cgcgctttgc gtgctcgccg gcgactccga aggcacccat
     2221 acatagaacc tgggactttt caattgcttc ttctctttgt ccgagtattc atatccaaac
     2281 atattgcggg caggtatcgc accccatgag cttgcgtgta aacggcggtc agcctgcctt
     2341 tccggaccat gctcgttgcc gtctctgaac agtggccctt tggccgagaa ctgtgtatcc
     2401 gggggcacgg ggaaggctat tgccacatgg tcgttcgaaa cggcaacgac aatgatcccc
     2461 cggttggccc gttgctggac gtcttcctcc tgaacctcgc tccacgcact cttgtcggct
     2521 tctattcttg cttttatctg tgcagctttc aggcccttct tccacgctcc cgtaccagcc
     2581 ggcagaccaa gtctgttgag ctcttttcct acgccctgaa aaaaaagatt acatactagc
     2641 ctgggttcca ggtccttatg atatccccct ttgggtcgat tgagctcctt ggtcaccttg
     2701 tccatgatat tgacgagatt cgcagcggcc gggggaaatc tcacatcctc gggttttgtc
     2761 cctccggccg gttccgccac gcgcacgcac acgccgcccc ggttctcgaa ccccgggata
     2821 cagcggcaat ggacgacgtt gtctttttcg gagacgggcg cggaattggg cgggcagccc
     2881 tgtgccgcgg ctgctgaagt gtacaccatc gcggcgaatc cgatcatgcc gcccgttgcc
     2941 aggagaaggg ttcttctcat ctatttgtcc agcacgtaga tcttctcttt cagtttggtc
     3001 tcggccttct tgaactcctc aactgccgcg ttgagcttca gctgggcctg atcgacgtcc
     3061 ttctgtgtcg tcttgccggc ctgcgcgtcg gccttcgccg cattgagcgc cgcgtccgca
     3121 ttcgcaagcc tgttgtagga gatgatctct gcctgcctga gcgccctgac ttcgcggttg
     3181 cccatgaacc tgtcgacggt ggctgccggc atgggtttca ggaactcctg ttcggggagc
     3241 ttcagacgag acaccttgag gctctgcgta ccgctgtcga aaagaccccg gagttttgcg
     3301 aggctctgct ggatctgggg gtcctgcggg tctctccggt tcgcctcttc gagatacagt
     3361 ttggcctgtg cgagatcacc gttcctcatg gcgaactcgc ctttctgcct cagagttatc
     3421 ggggtatcgg cgatcgcgcc cagctgaccg gcgagcttgc tgtcaaccgg ggccaatcct
     3481 gccatttgcg acccctcggc gctgggacgg cgccagacga tgacgggcgg gcccatgatg
     3541 acggcgcgtc cgcctccgcc ttctgttccg gggcacggtt tattgagggg aaaacctccg
     3601 accagcttgc cgttgatcca gcactgtgcg ctcaccgcct gtccgccatt accggcgtgg
     3661 gcatccggag ggagcgccgc ggcgagaacg agtatgagga ggaccgctat gcccggtacg
     3721 gcagcggtac cccacccgca ccgttcgcct tgaccgtgca cggacctttt ggacacggcc
     3781 cggcgcatcc ttaatacctg gcacgggcgc gcatgaccac ctgagtggcg ccctgggcgg
     3841 ggatcatggc gacggtgtat gtcccgcgcg gaatgctgac ggaaacggag tcaactccgc
     3901 tgacatgcgt gcggaggatc ggcgagccgg acggtttttc acctgaactg atggccttcc
     3961 attggtcttc cgtgatgacc atgaggagca gcttctttcc gggctgaacg gctacgtcga
     4021 agaagaccgt tgcctgctgg gggacaaaaa ggttcgaggc gctgccgttc tcgttgaggt
     4081 tcacgttctg gttgagaagc acgcgatccg aaggtcctac ggggatcgaa tcgaggggcc
     4141 cggcaaaggt ctggcccgct gtgaagagca gagcgcaggc aataagggcg atgactccga
     4201 gggcccgaag agacccgagc accgtgcggt cagaacgcgc gggcgcagac ccggtaggtg
     4261 acggacacag cctggggatt tgaattaaga attgcgacat agtaacctcc ctggttcacg
     4321 gtcagtgact ggcttgcagc cccatcaatg ggtgcacgga cctgcggccg cccggtgatc
     4381 tgttttcccg catccatttc cctcttctgc gcgcctgtta tgaccagcag ggtaacctgc
     4441 ctgcccggct gcacggcaaa atctatggag agaaaagcac ggctggagac atggatgtcc
     4501 gatccgacag caccgttcgc ggagaccgtg acg
//
LOCUS       DAOPOA010000097         4497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_20981_length_4497_cov_3.269923, whole genome shotgun sequence.
ACCESSION   DAOPOA010000097 DAOPOA010000000
VERSION     DAOPOA010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20981_length_4497_cov_3.269923"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PKJ17_03140"
     CDS             complement(<1..448)
                     /locus_tag="PKJ17_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD74995.1"
                     /translation="MTTEEDTPAIRTESLTKSFGDLKALDGITLEVHKGELFGLVGPD
                     GAGKSTLMRLLAAVMEPTSGNAWVASHPIGKGAERIKEKIAYMPQRFGLYEDLTVMEN
                     MVFYADIFDVPKAERAARMDRLLGFSGLVPFTGRLAGQLSGGMKQKL"
     gene            complement(445..1437)
                     /locus_tag="PKJ17_03145"
     CDS             complement(445..1437)
                     /locus_tag="PKJ17_03145"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD74996.1"
                     /translation="MISKKKIIPLAVIAAAVIVAVIVVMSFVRGRDDGTMKLSGNVEV
                     TECNVGFKVAGKIRALKVDEGARVKEGDLIAELSSGDVKALVDQNRAALEEARVKLAE
                     LKAGSRRQEIVKARADSASLEAELVKARKDFERAGTLYENGAISASRFDAAKSAYETR
                     LGQLKSARQQQSLVEEGPRREDIRAAELRVKQLEALVANTEEKLADTRLYAPISGVVF
                     RKNVELGEIVQPGAAVFTIGDLDRPWVKVYVKEDKLALVKLGQKARITVDTYKDRSYE
                     GTVTFISSDAEFTPKNVQTQEERVKLVFGVKVTVTNRDQELKPGMPADVRISLK"
     gene            complement(1434..2834)
                     /locus_tag="PKJ17_03150"
     CDS             complement(1434..2834)
                     /locus_tag="PKJ17_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD74997.1"
                     /translation="MTWIARLFIVPALAAIVLVASAAVAQEPFTLPSLLAYAAKHNPA
                     LRVTQRNVEIEGEGIRAAQADRMPRIDLTGGYTRYRYPAPLTPIVITSLPMLASDLPD
                     FEKNVYDGFATFSVPLFKGGRLMRGVTIAELKRSMARDFHTRNLHELTYNVTAVYYKL
                     AQLHELLRASDAQVKGLESHRRDVKLALKAGTAPKLDLLKADTELGAAIERKIQTRNA
                     VTNTRELLKTLIGMDDPGDPDIVFEDNPSMGGAVAKADLEGALASRPDYRAAQSKVRM
                     LEERVASAQGKRLPDIYGAGDYGGKAGDRMSFKENWSVGVRLFLPIFDFGRIGAEVDR
                     ERLELMKAKEEQRALRLTIVRELKEAETSIVDADERIAVSEKAVLSAKEQVRVEDLRY
                     RSGDNTSTDVINAEAALIRSRADLCQARFDRRVAVASLDKAMGTMDLPAQAQGKGNSP
                     GANGAAPGRPMTEEVK"
     gene            complement(2831..3475)
                     /locus_tag="PKJ17_03155"
     CDS             complement(2831..3475)
                     /locus_tag="PKJ17_03155"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HOD74998.1"
                     /translation="MEDRPNTRQRILEAAGEIFADSGFRQTTVRQISARAGVNVAAIN
                     YHFQSKDNLYLETLRYWKDVAFTKYPGEPGTSEADEPEKRLEGFIRAFVFRILDGGVE
                     SRFGRLMAREFAEPTAALDVIVEETARPIFHLITALVGRIIGRDPTSDTVLYCCASIV
                     GQCLYFLYARPVLRRLVGPERADSMDMADIAAHVCRFSLAALATIRHDPEGSSS"
     gene            3889..4455
                     /locus_tag="PKJ17_03160"
     CDS             3889..4455
                     /locus_tag="PKJ17_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD74999.1"
                     /translation="MDTINLTEKTDWAFIEEVKKESGQNPSLCYQCGNCTAGCPYTQY
                     FDYPVSMVMRLLQAGQKETILTSKAIWLCATCETCTTRCPCEVDVAHIMDTLRIIARR
                     EGKVSEKDVQLFYDSFLDSMKKHGRIFEMGILMGYKFKSGQFMGDAELGPKVMSKGKI
                     SLFPKTIKGADKVAQIFNRFQEKAKKHG"
BASE COUNT          830 a         1316 c         1358 g          993 t
ORIGIN      
        1 cgagcttctg cttcatgccg cccgagagct gtcctgccag cctgccggtg aaaggcacaa
       61 ggcccgagaa cccgaggagc ctgtccatcc gggccgcgcg ctcggccttc ggcacgtcga
      121 agatgtcggc gtagaagacc atgttctcca tgaccgtgag gtcttcatac aatccgaagc
      181 gctggggcat ataggctatc ttttccttga tcctctcggc acctttgcct atgggatgcg
      241 aggccaccca ggcgttgccg gacgtgggtt ccatgactgc cgcgaggagt ctcatgagtg
      301 tcgacttccc cgcgccgtca gggccgacga gcccgaagag ctcgcccttg tggacctcga
      361 gagtgatccc gtcgagcgcc ttgaggtcgc cgaagctctt ggtgagggat tccgtgcgta
      421 tcgccggggt atcttcttcc gtcgtcattt gagagagatc ctcacgtcgg cgggcatgcc
      481 gggtttgagc tcctggtccc tgttcgtgac ggtgaccttc acgccgaaga ccagcttgac
      541 gcgttcttcc tgggtctgga cgttcttcgg agtgaactcg gcgtcggagg agatgaatgt
      601 gaccgtgccc tcgtaggacc tgtccttgta tgtatcgacg gttatcctgg ccttctggcc
      661 gagtttgacg agggcgagct tgtcttcctt cacgtacacc ttgacccagg ggcgatcgag
      721 gtcgccgatg gtgaatacgg ccgcgccggg ttggacgatc tcaccgagct cgacgttctt
      781 cctgaagacc acccccgata tgggcgcgta gagccgggtg tcggcaagct tctcttccgt
      841 gttcgcgacg agggcctcga gctgcttcac ccgaagctcg gcggccctta tatcctccct
      901 gcgcgggcct tcctcgacga ggctctgctg ctgcctcgcg ctcttcagct gaccgaggcg
      961 cgtttcgtag gcgctctttg ccgcgtcgaa gcgcgacgcc gatatggccc cgttctcata
     1021 gagggtcccg gcgcgctcga aatccttgcg ggccttcacg agctccgcct cgagggacgc
     1081 cgagtccgcc cgggccttca cgatctcctg tcgccgcgag cccgctttca gctccgcaag
     1141 cttcaccctg gcctcctcga gggcggcgcg gttctgatcg acaagggcct tcacgtcgcc
     1201 gctcgagagt tcggcgatca gatcgccctc cttcacgcgg gcaccttcgt cgaccttgag
     1261 ggccctgatc ttgccggcga ccttgaagcc gacattgcac tccgtcacct ccacgttgcc
     1321 ggagagcttc atggtgccgt cgtcgcgtcc gcggacaaag ctcatgacga ctatgacggc
     1381 gacgatcacc gcggcggcga tgaccgccag gggaatgatt tttttcttgg agatcatttc
     1441 acttcctccg tcatgggtct tccgggggcg gcaccgtttg ccccgggact gttgcctttc
     1501 ccttgcgcct gtgccggcag gtccatcgtc cccatcgcct tgtcgaggga cgccacggcg
     1561 acgcggcggt cgaagcgcgc ctggcagagg tcggcccggg aacggatgag ggcggcctcg
     1621 gcattgatga cgtcggtgct cgtgttgtcg cccgacctgt accgcaggtc ctcgacgcgc
     1681 acctgttcct ttgcggaaag gaccgccttt tccgagacgg cgatgcgttc gtcggcgtcg
     1741 acgatggagg tctcggcctc cttgagctcc ctcactatcg tgaggcgcag ggccctttgc
     1801 tcctccttcg ccttcattaa ctccagacgc tcacggtcga cctccgcccc gatgcggccg
     1861 aaatcgaaga tgggtagaaa gaggcgcacg ccgacgctcc agttctcctt gaaggacatc
     1921 ctgtcgcccg ctttcccgcc ataatctccg gctccgtaaa tatcgggcag tctcttgccc
     1981 tgagccgagg cgacgcgttc ttccagcatc ctgaccttgc tttgcgccgc cctgtagtcc
     2041 ggccgtgagg cgagggcgcc ctccaggtcc gccttcgcga cggcgccgcc catggacggg
     2101 ttgtcctcaa agacaatgtc ggggtcgccg gggtcgtcca tgcctatcag ggtcttcagg
     2161 agttccctgg tgttcgttac ggcgttcctg gtctgtatct ttctttcaat ggccgcgccg
     2221 agctccgtgt cggccttgag gagatcgagc ttcggtgccg ttccggcctt gagagccaac
     2281 ttcacgtccc gcctgtgcga ctcgagaccc ttcacctgcg cgtcagaggc gcgaagcagt
     2341 tcgtggagct gggcgagctt gtagtagaca gcggtaacat tgtaggtcag ttcgtgaagg
     2401 ttgcgggtgt gaaagtcgcg ggccatggac ctcttgagct cggcgatggt gacgcccctc
     2461 atgaggcgcc cgcctttgaa aagggggacg gaaaaggtgg cgaagccgtc atagacgttc
     2521 ttctcgaagt cgggcagatc gctggccagc atgggcaggc tggttatcac gatgggcgtg
     2581 agtggggcgg ggtaacggta gcgcgtgtat cccccggtca ggtctatccg cggcatccgg
     2641 tcggcctgcg cggcccgtat gccctcgccc tctatctcca cgttccgctg cgtcaccctg
     2701 agggcggggt tgtgcttcgc ggcataggca aggagcgagg gaagggtgaa aggttcctgt
     2761 gccacggcgg cgctcgcgac aaggacgatg gccgcgaggg ccggaacgat gaacaggcgt
     2821 gcgatccatg tcatgacgat gatccctccg ggtcgtgtct tatggtggca agggccgcga
     2881 gtgaaaagcg gcagacatgc gcggctatgt cggccatatc catggagtcc gcccgctcgg
     2941 gtccgacgag cctcctgagg accggccggg cgtagagaaa atacaggcat tgcccgacga
     3001 tgctggcgca gcagtagagc accgtgtcgc ttgtcgggtc gcgtcctatg atgcggccga
     3061 caagagccgt gatgagatga aaaatgggcc gcgccgtctc ttccacgatg acgtcgaggg
     3121 ccgctgtcgg ttcggcgaat tcgcgggcca tgagccttcc gaaccgcgat tcgactcccc
     3181 cgtcgaggat gcggaagacg aaggcacgga tgaagccttc gagccgcttt tcgggttcat
     3241 cggcctcgga tgtccccggt tcccccggat atttcgtgaa ggccacatcc ttccagtacc
     3301 gcagcgtctc caggtagaga ttatccttgc tctgaaaatg gtaatttatt gctgcgacgt
     3361 tcacgccggc acgggcgctg atctgcctta cggtcgtctg gcggaacccg gagtcggcga
     3421 agatctcacc cgccgcctcg aggatgcgct gcctcgtgtt cggtctatct tccatcgccg
     3481 ttccaccgtt tcatattaaa cagtagtttc atacaactat tttaaactag cgttggagat
     3541 tgtcaagaaa tttttttcac aatagccttg tgaagacaat tacatactcc ggtcaaaaaa
     3601 gcccctgtgt tatcaacagg atgagggggg ttaacaaaag gagtattcat tattctgaat
     3661 ggttggcgtg gcaaatatgt attgacaagg aaaatccaat tagtgttaaa taccaacagg
     3721 ttttaagggg tatagttcat tgataagcag tctcgttctg atggttcact aatagtgttc
     3781 actatagtga atatattttc cggagcagtc attgacgagt tcacgggcac aattcgacgt
     3841 acggttaacg ggctcactgc tgccggctct aaaggttggt taatctgaat ggatacgatc
     3901 aatttaactg aaaaaaccga ttgggctttc attgaagagg tgaagaagga gagcggtcag
     3961 aacccatcgc tgtgctatca gtgcgggaac tgtaccgccg ggtgtcccta cacccagtac
     4021 ttcgactatc cggtcagcat ggtcatgcgc cttttgcagg ccggacagaa ggaaacgatc
     4081 ctcacctcca aggcgatttg gctctgcgcc acctgcgaga catgcaccac ccggtgtccc
     4141 tgcgaggtcg acgtggccca tatcatggat acgctgagga tcatcgcccg ccgcgagggc
     4201 aaggtctccg agaaggacgt tcagcttttc tacgattcat tcctcgattc gatgaagaaa
     4261 cacggcagga tcttcgaaat gggcatcctc atgggctaca agttcaaatc gggccagttc
     4321 atgggcgatg cggaactcgg tccgaaggtc atgagcaagg gcaagatcag cttgttcccc
     4381 aagacgataa aaggcgccga caaagtagct cagattttta atagattcca ggaaaaggcg
     4441 aaaaagcatg gctgagaaag aatacgcata tttcagtggt tgttcgctgg aaggtac
//
LOCUS       DAOPOA010000098         4475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_21119_length_4475_cov_3.157692, whole genome shotgun sequence.
ACCESSION   DAOPOA010000098 DAOPOA010000000
VERSION     DAOPOA010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21119_length_4475_cov_3.157692"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="PKJ17_03165"
     CDS             <1..1329
                     /locus_tag="PKJ17_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HOD75000.1"
                     /translation="MIVLPDSPMFIYAFLGSIKYGAWPIPVNTMLNEEDYRYMLENSQ
                     AKVLVTWSQSKDSPVRPEHFCYKLLDDNEFITLMDNASTEAEAHPAREDDIAFWLYSS
                     GSTGKPKGVPHKHIDMIHSADNYGTGVLGIREDDICFSVSKLFFAYGLGNGLHIPFRH
                     GASTVLLSSPPSPEAVMETVVTFRPTIFFGVPTQYNSVLKRMNGSGPDDFRSIRLCLS
                     AGEALPPEIFRKWQEKTGLEILDGIGSTEALHIFISNMAGDVRPGTSGRVVPGYEAKI
                     VDDLGNELPPGEAGHLVISGRSITPGYWMRPDDNAEKFIPGGWFKTGDMYTQEDGYFT
                     YQGRGDDMLKVGGIWVSPVEIENTLIQHDAVNEAAVVGHEIEGLSKAFGYISLNENYR
                     DKAADESLKDEILQFVNGRLPKYKSLRSVFFIDELPKTATGKIQRFRLRK"
     gene            1478..1699
                     /locus_tag="PKJ17_03170"
     CDS             1478..1699
                     /locus_tag="PKJ17_03170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD75001.1"
                     /translation="MSDEDHNIHDFDIALICEYFSSMDRQGPGSPEATLRALSFIGNM
                     REGSSIADLGCGTGGQTMVLAQKVTSCKT"
     gene            1783..2244
                     /locus_tag="PKJ17_03175"
     CDS             1783..2244
                     /locus_tag="PKJ17_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD75002.1"
                     /translation="MDVFADSHSWDMKADTLKADGDWPGLITHCRMWLEKYPEEIGAQ
                     ILLGEAYNHAGRYRESLDLYLKVAAKRPDDYTAWYTLGSIAVKAGKTQEAIDYLSMAV
                     YTNRDIAPAIKSAYPELYQKVKSKGGSNRILRLCVGMIILILFALAVWIFL"
     gene            complement(2241..3320)
                     /locus_tag="PKJ17_03180"
     CDS             complement(2241..3320)
                     /locus_tag="PKJ17_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75003.1"
                     /translation="MRDVKGVSSSGFKIDVFRPGDAVGVSGLFKEVYGDGYPVKIVYD
                     PEEFTRAVDRQEYIPVVARTPEGGIVGFSSFYRSAPNKRLYEMGLSMVSAGYRSTAVL
                     GLLVRRLVKVALASPCLDAFFGEVVCNHVLMQKSSGVFKAVETAIEIDLMPSEAYEKD
                     ENVSGRVSTVVSTRTIVPRPHTIRVPEVYGDYLAYIYNAFDDSRAFERSDERLPDDRS
                     TEFTVQIFEGAGVARITVNEAGSDFETVLDSEEKAARSRGVTVTQVWLNLSWPWVGGC
                     VEALRSRGYFFCGPFPRWFGEDGLLMEKVEGRPNWEGIILYSERARQILEFIKEDWAE
                     VTRKIEGGTEKRENVETRREPPKSS"
     gene            complement(3876..>4475)
                     /locus_tag="PKJ17_03185"
     CDS             complement(3876..>4475)
                     /locus_tag="PKJ17_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HOD75004.1"
                     /translation="YKNDLFYLSGIAAYGYNRYDTRRDIAFGTISRRAKGYYTGQTVG
                     GYVEAGHRVATPSVDIIPLGSLTGIHLMKDGFTERAAGALSLDVDPDRASSLVSSLGV
                     RLMKDYEVSPGILTPELRVTWDHELMNGDYSLDASFAGYPTSTFAVEGDRPDRDSLGV
                     GLSLRFQTEENLNLHLAYDGSFSGDNTQHAAIGGITYRW"
BASE COUNT         1070 a         1256 c         1209 g          940 t
ORIGIN      
        1 atgatcgtcc ttcccgattc acccatgttc atctacgcct tccttggaag catcaaatac
       61 ggagcctggc caattccggt caacaccatg ctgaatgaag aagattaccg gtacatgctc
      121 gagaacagcc aggcaaaggt tcttgtgacc tggagccaga gcaaggactc acccgtcagg
      181 ccggaacact tctgctacaa gctccttgac gacaacgaat tcattaccct tatggacaac
      241 gcatcaacgg aagccgaggc acatcctgca cgagaagacg acatcgcctt ctggctctac
      301 agttccggca gcaccggcaa acccaaaggc gtaccgcaca agcacataga catgatccac
      361 tctgccgaca attacggcac gggagtgctc ggcatacggg aagatgacat atgtttctcc
      421 gtgagcaaac tcttcttcgc gtacggtctc ggtaacggcc tccacattcc tttcaggcac
      481 ggggcttcca cggtactgct ttcttccccg ccaagccctg aggcagtgat ggaaaccgtc
      541 gtcacgttca ggcccaccat ctttttcggc gttcccacgc agtacaactc ggtattgaag
      601 agaatgaacg gctcaggccc tgacgatttc aggtccatcc gcttatgcct ttccgcgggt
      661 gaagcgctcc cgcccgagat cttcaggaaa tggcaggaga agacggggct ggagatactc
      721 gacggcatcg gctccacgga ggccctgcac atcttcatct ccaacatggc cggcgatgtg
      781 aggcccggaa cttcaggccg cgtggtcccg ggatacgagg caaagatcgt cgatgatctc
      841 ggcaatgagc tccccccggg tgaagcgggc catctggtga taagcgggag gagcattaca
      901 ccggggtact ggatgaggcc ggatgacaac gcggagaaat tcatccccgg gggatggttc
      961 aagacgggtg acatgtacac gcaagaagac ggttacttca catatcaggg aaggggcgac
     1021 gacatgctca aggtcggcgg gatatgggtt tcccccgtcg agatagagaa cacgctcatc
     1081 cagcacgacg ctgtaaacga ggccgccgtc gttggacacg aaattgaagg gctgagcaag
     1141 gccttcggct acatctcgct caacgagaac tacagggaca aggccgcgga cgaaagcctg
     1201 aaggacgaga tccttcaatt cgtcaacggg cggctgccca aatacaaatc gctccggagt
     1261 gtcttcttca tagacgaact tccaaagacc gccacgggga agatccagcg gttcagactg
     1321 agaaaataaa gcgccaatca ccggcgcgac tcccggtatg agtgcagggg cgggcaaccg
     1381 cccctcactt gtataacctg catcgatacc gggaaagatc gccctcatct gtaccacgtc
     1441 aacgcgctgt gctatactgg tctcggagga acacagggtg agcgatgaag accataatat
     1501 tcacgatttt gatattgcat tgatttgcga atatttttcc agtatggatc gtcaaggccc
     1561 gggaagcccg gaagcaacgc tgagggcgtt aagctttatt ggcaatatgc gcgagggatc
     1621 gagcattgcc gaccttggct gtggtactgg aggtcaaacc atggttttgg ctcaaaaggt
     1681 cacttcctgc aagacatgag aatgtaaaaa tgcaagggcc ccaaagtcat actgctttga
     1741 tgacgcggtc caaattcttg caggataagg gagataatgg ttatggatgt attcgctgat
     1801 tcccattcct gggatatgaa agcggacacg ctcaaagctg acggggattg gcccggcctt
     1861 atcacgcatt gccggatgtg gctggaaaaa taccctgaag agatcggggc ccagatcctt
     1921 ctcggggagg catataacca tgcaggccga taccgggagt ccctggacct ctatctcaag
     1981 gtcgctgcaa aaagacccga tgattacaca gcgtggtata cgcttggttc catagccgtc
     2041 aaagccggga agactcagga agcgatcgac tatctctcaa tggcggtgta cacgaaccgc
     2101 gacatagcgc ctgcgatcaa atcagcgtac ccggaactct atcaaaaagt aaaaagcaaa
     2161 ggcggttcga atagaatatt gcgactgtgc gtcggaatga tcatcctcat cctgttcgcg
     2221 cttgcggtct ggatattcct ctagctggat tttggaggct cccttcgcgt ttccacattc
     2281 tcccgtttct ctgtcccgcc ctctatcttc cgggtcactt ccgcccagtc ttccttgatg
     2341 aactcgagga tctgccgggc ccgctcggaa tagaggatga tcccctccca gttcggccgt
     2401 ccttcgacct tttccatgag aagaccgtct tcgccgaacc atctggggaa gggtccacag
     2461 aagaaatagc ccctcgagcg caaagcttcg acgcatcctc cgacccacgg ccaggaaagg
     2521 ttgagccata cctgcgtcac ggtaacgccc ctgcttcggg ccgccttttc ttccgagtcc
     2581 aggacggtct cgaaatcgga gcccgcctcg ttgacggtga tccttgccac ccctgcgccc
     2641 tcaaagatct ggacggtgaa ctcggtggac cggtcgtcag gcagcctttc gtccgaccgc
     2701 tcgaaggcgc ggctgtcatc aaatgcgttg tagatgtacg ccaggtaatc gccgtagacc
     2761 tcgggaacac ggatagtatg aggccgggga acaatggtcc ttgtggaaac gacagtcgaa
     2821 acccgtcctg aaacgttctc gtctttttca taggcctcag aaggcatgag atcgatctcg
     2881 atggcggtct cgacggcctt gaaaacgcct gagctttttt gcatcaggac atgattacac
     2941 accacctccc cgaagaaggc gtccagacaa ggcgatgcaa gagccacttt cacgaggcgg
     3001 cgcaccagga ggccaaggac agcggtgctc cgatacccgg ccgagaccat gctgagtccc
     3061 atctcgtaga gtctcttatt cggagcggac cggtagaaag acgagaagcc cacgataccc
     3121 ccctcaggag tacgggcgac cacagggata tattcctgtc tgtcgaccgc cctcgtaaac
     3181 tcttcagggt catagactat ctttaccgga tatccatcac catagacttc tttgaaaagc
     3241 ccggagaccc ccaccgcgtc gccagggcga aacacatcga tcttaaaccc ggagctgctc
     3301 acgcccttta catccctcat tattatcgat tcatcccgcg caattgtagc ccagacagaa
     3361 tcaataagca accttcgcca taaggcatga cgaaggacgc gccgggcttc aaccactttt
     3421 ctgcctcgct ttacgaccag ctcatcgcgg cggtggaaag acacggatgc cgaatatgaa
     3481 gatgtgaaga gtgtccctaa ggttctccaa tcaatcatat aaaacggata cgtttatcaa
     3541 aagattgtga cgtataaatc tatattcgat gacagagacg gtcttctgaa tatgataact
     3601 ttcccatatt gggacagagg attttggggc ccttcacatt ttcacattcg gcatccgtgc
     3661 aagaccaaag gaacatcgtg taagcatgga atcggaacat cgaaaggagg cgcggtcgtc
     3721 attgacattc tcgggagaga ggaaaaccat ctgcccttat accaccgata accatcattg
     3781 ctcgttcaga tccggtagaa gagagaatgt gaagatgtcc ctaaagttcc cctctgtcct
     3841 ttccgggggg attccggcga accgtgattc ccttgttacc acctgtatgt gatcccccct
     3901 atcgccgcgt gctgcgtgtt gtccccggag aagctcccgt cgtaggcgag gtgaaggtta
     3961 aggttctcct cggtctgaaa tctgagggag aggcccacgc cgagggaatc ccgatcgggc
     4021 ctgtcgccct ccaccgcgaa ggtggacgtc ggatagcccg cgaaggaggc gtcgagggaa
     4081 tagtctccgt tcatgagctc gtggtcccag gtgaccctca gttcaggggt gaggataccc
     4141 ggggatactt cgtagtcttt cataagtctc acgcccagtg aagacacgag tgaggaggct
     4201 cggtcggggt caacatcaag ggagagcgcc ccggccgccc tctcggtgaa accatctttc
     4261 atgaggtgga tgccggtgag ggatcccagg ggaatgatgt cgacggacgg ggtggccacg
     4321 cggtgccccg cttcgacgta tcccccaacc gtctgaccgg tgtagtaacc cttcgccctg
     4381 cgtgaaatgg tcccgaaggc gatgtccctt ctcgtatcgt agcggttgta cccgtaggcg
     4441 gctatgccgc tcaagtagaa aaggtcgttc ttgta
//
LOCUS       DAOPOA010000099         4459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_21229_length_4459_cov_3.023842, whole genome shotgun sequence.
ACCESSION   DAOPOA010000099 DAOPOA010000000
VERSION     DAOPOA010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21229_length_4459_cov_3.023842"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..772)
                     /locus_tag="PKJ17_03190"
     CDS             complement(374..772)
                     /locus_tag="PKJ17_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75005.1"
                     /translation="MGKAIPVNLLRMWELLSEDIQDALLITVEKNRAAHRNFRGTPVS
                     SCPHCGETDTMDCSTIDAVGDATVGLCLVCGYLWCLECDSSLLTGINCGHWQVCSSCT
                     EKKQSTGYCGTEPLECPRIREWLRKNHPVV"
     gene            complement(993..2114)
                     /locus_tag="PKJ17_03195"
     CDS             complement(993..2114)
                     /locus_tag="PKJ17_03195"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD75006.1"
                     /translation="MVLYFTQRWIFGPLIPSLMEAFGTDKTTLGIVGAGSLWGYMLTP
                     IIAGLISDRFGRRYVVLFGMFGLSAMTVVSGLAHSTGQLFLFRFVTGLIEGFFFIPMI
                     AFTMELFPERPGFYVTFMSSGTSFGWFTGPALAGWLLDLTGNWRIPFMTTGIAGMILA
                     AVLLAVWPEEKRERHPGGKLLDPSILTRSSLIMLVLVSCTAMFQISGEFGFAMWYPVF
                     LKTEMGMTASMAGLVAGFWGVGQFIGRPTMGHISDRLGYRKVGITGGIIMGLCLMLIL
                     KIDNPVARGSITFITGFVGCAVMGSLWTFTGLTFARTRGLALGVLTTWSYAIASLSPI
                     TIGWIGDHYRVSTGLFVICVPVVFLACATFAATNLVKKQ"
     gene            complement(2156..3358)
                     /locus_tag="PKJ17_03200"
     CDS             complement(2156..3358)
                     /locus_tag="PKJ17_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOD75007.1"
                     /translation="MFDSDTLDRYADVLLWGLTAARKGRFRKGDVIFLQYDPPATTLA
                     EVLYGKILDRGMYPLQRMGLTTFMEHAFYEKATDRHLGWLPPGDRELYEKVNGRIYLR
                     APESLTHLKDIDPMRISKVLVSRKVFRDILDKREEKGVYSWTLCTYPTAGLAKQAKMS
                     VAAYARQIVKACFLDEKDPVRRWDAVHAEATRIKKWLTGLKVRYLRVNSKNIDLVLTP
                     GENRKWAGISGHNIPSFEVFLSPDWRGTEGTYYANLPSFRSGNYIEGVRLVFRKGKVV
                     EADAKKGAEFLRKQVAMDKGASRVGEFSLTDKRFSLIDRFMADTLFDENFGGSEGNCH
                     LALGASYSDTFSGNPAKLTGEMKRKLGFNDSALHWDLVNTEAKTVTARLAKGREIVIY
                     EKGAFCLP"
     gene            complement(3351..3722)
                     /locus_tag="PKJ17_03205"
     CDS             complement(3351..3722)
                     /locus_tag="PKJ17_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75008.1"
                     /translation="MKISFVVPTGDIVNEERDLFLTHAMGGCWHEDGGGAPVTKFALK
                     GHICSKCGLFYTTRNDFSTLEDFLKLYEWARGDPGLEDFVAQFRTRDLVNEKRGPGAR
                     KRFADGLYRILTARGRETGNV"
     gene            complement(3761..>4459)
                     /gene="amrA"
                     /locus_tag="PKJ17_03210"
     CDS             complement(3761..>4459)
                     /gene="amrA"
                     /locus_tag="PKJ17_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOD75009.1"
                     /translation="PAVVASMMAATKMGAEAEVLHYTNSGDTAGDRTRVVGYGSLAFW
                     QPDGKAASSPLAAAERKRLLAIARKTLEEYVSSKTVPEIDVKDPRLLERRGVFVTLTI
                     HGQLRGCIGYIKPVAPLYRSVVDMTVAASSRDMRFRPVTKGELKDIEIEISVMSPLKL
                     ITSVDEIKVGIHGLYIVKGGNAGLLLPQVATQYKWGREEFLQNTCRKAGLPENSWKDK
                     ETKVYVFSAQIFSE"
BASE COUNT          914 a         1310 c         1298 g          937 t
ORIGIN      
        1 cgaaccgcgg tgggtcgaat actcctctcc gtggaacttc cgaagctccc agtctatctt
       61 ccctacccag tgaacgttcg gagctatttc aaatttcatg acacccctcc tctttcactc
      121 tgattgctgt acctgtgccc cattttactc cagatcatct tgtaagtcac ttgacttaag
      181 tcaagagccc cgggaaaaga cagttgaagc cgcttgaaca gcttgaaccg cttgaacgga
      241 aaaggccagg aaagacaaaa atggttgtaa ccgcttgaat gacgaagacg aaaaggatct
      301 ccggatcaaa accgggatga cgacagtgtc ccactttttt ttgcctctga cgttcaagcg
      361 gttcaatccg tcttcaaacc accggatggt ttttcctcag ccactccctg atgcgggggc
      421 attccagggg ttcggtgccg cagtagccgg tggactgctt cttctccgtg catgatgaac
      481 agacctgcca gtggccgcaa ttgataccgg tgaggaggga cgaatcgcat tccaggcacc
      541 acaggtaccc gcagacaagg cacaggccca cggtcgcgtc gccgacagcg tctatggtgg
      601 agcaatccat cgtgtccgtt tcaccgcagt gggggcagga agagaccggt gttccccgaa
      661 aattcctgtg tgccgcgcgg ttcttttcga cggtgatgag gagcgcgtcc tgaatgtctt
      721 cggagaggag ttcccacatt ctcaggagat tgaccggtat cgccttgccc atggctcgag
      781 actccttctg gggagatttg gagtggaata cgaacattat tgtatcacaa atggaacagg
      841 aaccaaatga gaacataatg accgattcac caatagccaa ttgaccaaag aataaggaca
      901 gagcggaccg ttttgattcc cccccgtctt ctgtctggtc attgattatt gatttattgg
      961 agattatctg gtcattggag tttggttatt gactattgtt ttttgacaag gtttgttgcc
     1021 gcgaaggtgg cgcaggcgag gaagaccacg gggacgcaga tgacgaaaag gcccgtggaa
     1081 acgcggtaat gatcgccgat ccagccgatg gttatggggg agagagaggc gatcgcgtag
     1141 gaccacgtcg tcagcacgcc aagggcaagg cccctcgtgc gggcgaaggt gaggcccgta
     1201 aatgtccaga gggagcccat cacggcgcag cccacaaaac ccgtgatgaa cgtgatggag
     1261 cctctggcga caggattgtc gatcttgagg atcagcataa ggcacaatcc catgatgatg
     1321 ccgccggtga tgccgacctt acggtacccc agcctgtcgg agatgtggcc catcgtcggg
     1381 cggccgatga actggccgac accccagaag cccgccacaa ggcctgccat cgaagccgtc
     1441 atgcccatct ccgttttcag aaagacgggg taccacatgg caaaaccgaa ctcccccgat
     1501 atctggaaca tcgcggtgca gctcacgaga acgagcatga taaggctcga ccgcgtgagt
     1561 atggacgggt cgaggagttt tccgccggga tgcctctccc gcttctcttc aggccatacg
     1621 gcaaggagca cggcggcgag gatcatgccg gctatgcccg tggtcatgaa ggggatgcgc
     1681 cagtttccgg taaggtcgag gagccaaccc gcgagggccg gtccggtgaa ccagccgaag
     1741 gacgtgcctg atgacatgaa ggtgacgtaa aacccgggcc tttcaggaaa gagttccatc
     1801 gtgaaagcga tcatggggat gaagaagaag ccttctatga ggcccgtcac gaaacggaac
     1861 agaaagagct gccccgtgga gtgggcgagg cccgagacga cagtcatcgc cgagagcccg
     1921 aacatgccga agaggaccac gtatctcctc ccgaacctgt cggatatgag accggcgatg
     1981 atcggtgtca gcatgtaacc ccacagcgag cccgcgccga cgatgccgag ggtggtcttg
     2041 tcggtgccga aggcttccat gagggagggg atcagcggcc cgaagatcca cctctgggtg
     2101 aagtagagga ccatccccac ccagccgaca aagaagaaga ggtgcttatg cgaccctatg
     2161 gaagacagaa ggcacccttc tcgtagatca ctatttccct gcccttcgca aggcgtgccg
     2221 tcacggtctt ggcctcggtg ttgacaaggt cccagtgaag cgcggagtcg ttgaagccga
     2281 gctttcgctt catctccccc gtcagtttgg ccgggttgcc gctgaaggtg tcgctgtagg
     2341 acgccccgag agcgaggtgg cagttcccct cggagccgcc gaagttctca tcgaagaggg
     2401 tgtcggccat gaagcggtcg atgagggaaa agcgcttgtc cgtaagggag aattccccga
     2461 cccgggaggc ccccttgtcc atggcgacct gtttccttag aaattcggcg cccttcttcg
     2521 cgtccgcctc gacgaccttt cccttcctga acacgaggcg cacgccctcg atgtagttgc
     2581 cgctccggaa ggatggcagg ttggcgtagt aggttccttc ggtgcctcgc cagtcgggtg
     2641 agaggaagac ctcgaagctg gggatgttgt gccccgagat gcccgcccat ttgcggttct
     2701 cccccggtgt gaggacgagg tcgatgttct tcgagttgac ccggagatac cgcaccttga
     2761 ggcccgtgag ccatttcttt atgcgtgtcg cctcggcgtg gacggcgtcc catctcctga
     2821 cggggtcctt ctcgtcgaga aagcaggcct tgacgatctg ccgggcatag gccgcgacgg
     2881 acatcttcgc ctgtttcgca agcccggcgg tgggataggt gcacagcgtc cagctgtaga
     2941 cccctttctc ctcccttttg tcgaggatgt cgcgaaagac ctttcgggat accaggacct
     3001 tgcttatccg catggggtcg atgtccttaa gatgggtgag ggactcgggg gcgcgcaggt
     3061 atatcctccc gttgaccttc tcgtatagtt ccctgtcccc cggcggcagc cagccgaggt
     3121 gccggtccgt tgccttctcg tagaaggcgt gctccatgaa ggtggtgagc cccatgcgct
     3181 gaagaggata cattccccgg tcgaggatct tcccgtagag gacctccgca agggttgttg
     3241 ccggggggtc gtactggaga aagatgacat cccctttcct gaaacgtccc ttccgggccg
     3301 cggtgagccc ccagaggagc acgtcggcat acctgtcgag tgtgtcgctg tcaaacattt
     3361 cccgtttccc tcccccgtgc cgtcagtatc cggtagagac cgtcggcgaa cctttttctt
     3421 gctcccggcc ctcgtttctc gttgacaagg tcccgggtcc gaaactgtgc gacgaaatcc
     3481 tcaaggcccg ggtcgccgcg cgcccactcg tagagtttca ggaaatcctc cagggtggag
     3541 aaatcatttc tcgttgtgta gaacaggccg cactttgagc agatgtggcc cttgagggcg
     3601 aacttcgtta caggtgcccc gcccccgtcc tcatgccagc agcctcccat ggcatgcgtg
     3661 agaaagaggt ccctctcttc gttgacgatg tcgcccgtgg gcacgacgaa actgatcttc
     3721 atgtgtgctc ccttaccagc ggttgaccgc tgaacatccc ttattccgag aatatctgcg
     3781 ccgaaaaaac gtagaccttc gtttccttgt ccttccacga gttctccggc aggccggcct
     3841 ttctacacgt attctgcagg aattcctccc ggccccactt gtactgggtg gccacctgcg
     3901 gcagcagcag gcccgcgttg ccccctttca cgatatacag accgtgaatc cccaccttga
     3961 tctcatccac cgaggtgatg agcttcaggg gtgacatcac ggagatctcg atctcgatat
     4021 ccttgagctc gccctttgtg acgggccgga acctcatatc cctcgacgac gccgcgacgg
     4081 tcatgtccac caccgacctg tagagaggag ccacgggctt gatgtagccg atgcatcccc
     4141 tgagctgccc gtgaatggtg agcgtcacga agacccctct cctttcgagg agcctggggt
     4201 ctttgacatc gatctcgggc acggtcttcg acgacacgta ttcctcgagg gtctttcgag
     4261 cgatggcaag gagccgcttc ctctcggccg cggcaagcgg tgaggaggcg gccttaccat
     4321 cgggttgcca gaaggccagg gaaccgtaac ccaccaccct cgtcctgtca cctgccgtat
     4381 cccccgagtt cgtgtagtga aggacctccg cctcagcccc catcttcgtc gccgccatca
     4441 tggaggcgac caccgccgg
//
LOCUS       DAOPOA010000100         4451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_21276_length_4451_cov_3.671292, whole genome shotgun sequence.
ACCESSION   DAOPOA010000100 DAOPOA010000000
VERSION     DAOPOA010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21276_length_4451_cov_3.671292"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /gene="phoU"
                     /locus_tag="PKJ17_03215"
     CDS             <1..499
                     /gene="phoU"
                     /locus_tag="PKJ17_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOD75010.1"
                     /translation="GAIAQFQPRARDLRVILMVYKMNSDLERMGDHVANIADSGLFLI
                     SHVPVKPLVDIPRLAGETTRSLNDSITAFLNEDASLAQQVCERDHIVDDLQNQIFREL
                     VTFMSSDPATIERSLHLIRVANNLERIADLSTNICEDVIFMVEGRVIKHHREERDEKQ
                     KGEKV"
     gene            complement(604..1821)
                     /locus_tag="PKJ17_03220"
     CDS             complement(604..1821)
                     /locus_tag="PKJ17_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75011.1"
                     /translation="MKKFLLALIAVALATPVLGNAGSVSSRYDVTFGGFVKYDFGYST
                     QNSHADPSYGYRSSTSDRAILADEYGNTYMSGAETRFNFLIKGPDLWGAKTSAFIEGD
                     FRGTTTGNQYGGFQLRHAWMKMKWNSSELLLGQAYQQWGMPYYAAQIGADDFKQYLKG
                     IRTPQVAFRHFFTKELSAMFGLTSATEWSGATRQYNDGYARSSWPGLQGEMAYWTDRC
                     GKIGSNNLKFGLGAYYGKDTETYTSGAEYKDSTVNAWVTAFRYSNGNFFIGQNAAGNN
                     WMGSSGPSNGSYLRPGNDAAAPTLFGLFSQVSWWMTDKLWVNGMYGYLKYNYSEWART
                     NNATARDRINMSQTFAANLLWEANPSIRLGLQWMRMFTHYNGVGQGTGAGTGLAASSG
                     VMDQYRFAAWYFF"
     gene            complement(2127..2396)
                     /locus_tag="PKJ17_03225"
     CDS             complement(2127..2396)
                     /locus_tag="PKJ17_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75012.1"
                     /translation="MKTGNVSQGSTLLGQTRSPRENRTARTKDSAPIEEKVDLSTKKA
                     PVIPSAATSYDDVLELLYGIDFSQFKNVEWISKDGSSKMMELLGA"
     gene            complement(2471..2740)
                     /locus_tag="PKJ17_03230"
     CDS             complement(2471..2740)
                     /locus_tag="PKJ17_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75013.1"
                     /translation="MGRKILDTTHLMTLPRTGQKSPFTPASAEHFADGQYLFVGLYEM
                     AVASFIDQAACEFQDYCNHLYCIDGRCDCPWSWKKSAGLALPASA"
     gene            3051..4202
                     /locus_tag="PKJ17_03235"
     CDS             3051..4202
                     /locus_tag="PKJ17_03235"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75014.1"
                     /translation="MIARKIQASVEESLKQFPVVGLIGPRQAGKTTLAKMILQKTETR
                     PLYLDLELSSDVNKLRDPEMYLRRYEDRLVIIDEVQRMPELFPLMRAMVDQKRSAGRF
                     LILGSASPALVRQASETLAGRAVYRELLPFSLDEIGCGNTDRLWLRGGYPLSYLAAGN
                     DESYSWREAFIRTYLEMDIPQLGIRVPALQLRRFWTMLAHSHGQLWNASKIAGSLGIS
                     APTAKHYLDILSDTFVVRQLQPFFANVKKRLIRSPKVYLRDAGLFHTLMRIKEKEDLD
                     AHPSAGASWEGFIIEQIIAAKPDDWEAYFYRTSAGAEIDLLLLDESGRPTAIEIKYSL
                     SPKPGRGFWSAIDDLACEKAFVIFPGDESYPLKENAFTLPVAQIQRIFH"
BASE COUNT          975 a         1221 c         1264 g          991 t
ORIGIN      
        1 gggggccata gcgcagttcc agccgagggc ccgggactta agggtcatcc tcatggtcta
       61 taagatgaac agcgacctgg agcgcatggg cgatcacgtg gcgaacatcg ccgacagcgg
      121 attgttcctc atatcccacg tgccggtgaa gcctcttgtc gatattcccc ggctcgcggg
      181 cgaaacgaca aggagcctca acgacagcat caccgcattc ctgaacgagg atgcgtccct
      241 cgcccagcag gtctgcgagc gggaccatat cgtcgacgac ctgcagaacc agatctttcg
      301 ggagctcgtg accttcatgt cctcggaccc ggcgacgatc gagaggtcgc tgcacctcat
      361 cagggtcgcg aacaacctcg agcgcatcgc cgatctttcc acgaacatct gcgaggacgt
      421 gatattcatg gtcgagggaa gggtcatcaa gcaccatcga gaggaaaggg acgagaagca
      481 gaaaggtgaa aaggtctaga cccgcaggcg tgacggcaaa gctcttcagg gattgcctcc
      541 cggggaacca ataaagaaaa ggggccttga aaaaggcccc ttttcatatc cgaacgggga
      601 ttcttagaag aaataccagg ccgcgaaccg gtactggtcc atgacgccgc tggaggcggc
      661 taaacccgtg cccgcgccgg taccctggcc cacgccgtta taatgggtga acatcctcat
      721 ccactggagg cccagcctga tcgacgggtt tgcctcccaa agaaggtttg ccgcaaaggt
      781 ctgggacatg tttatcctat cacgggccgt cgcgttgttc gtgcgcgccc actcgctata
      841 gttgtacttc aggtagccat acatgccgtt gacccagagc ttgtcggtca tccaccagga
      901 aacctgggag aagagcccga agagtgtcgg agcggccgcg tcgttgccgg gcctcaggta
      961 ggagccgttg ctcgggcccg aggagcccat ccagttgttc cctgccgcgt tctgcccgat
     1021 aaaaaagttt ccattggagt agcggaacgc cgtgacccag gcgttgaccg tgctgtcctt
     1081 gtactccgcg cccgaggtgt aggtctccgt gtccttgccg tagtaggctc caagaccgaa
     1141 cttgaggttg ttcgagccga tcttgccgca gcggtccgtc cagtatgcca tctctccctg
     1201 gagacccggc caggagcttc tggcatagcc gtcgttgtac tgccttgtcg cgccggacca
     1261 ctccgttgcg gaggtgagcc cgaacatggc gctcagctct ttggtgaaga aatggcggaa
     1321 ggccacctgg ggtgtcctga tacccttgag atactgcttg aagtcgtcgg ctcctatctg
     1381 ggcggcgtag tatggcatac cccactgctg gtaggcttga cctaagagca gttccgacga
     1441 gttccacttc attttcatcc acgcgtgcct gagctggaat ccgccgtact ggttgcccgt
     1501 cgtcgtgccg cggaaatcgc cctcgatgaa ggcgctcgtc ttcgcgcccc acaggtcggg
     1561 gcccttgata aggaaattga agcgggtctc ggcgccgctc atgtaggtgt tgccgtactc
     1621 gtcagccagg atcgcgcgat cggacgtgct gctcctgtac ccgtaggaag ggtccgcgtg
     1681 cgagttctgg gtcgaatagc cgaagtcgta tttcacgaaa ccgccgaatg taacgtcata
     1741 cctgctcgat acgctgccgg cattgccaag taccggagtt gccagggcga ccgcgatcag
     1801 ggccaataag aacttcttca ttcctgcctc cttagaaata gttgtggtgg tacggaacac
     1861 ggtgctgcct ttccctcctt tccgtcaatg tgggtttaac tgtcctgagg caagattaac
     1921 caaatttaat taagcgggcg ttaaatgcct gttaaattac cgttaagcgg agagaacccg
     1981 aaggaggccg tgaagggttc ttttcttgcc ccgtgaaggg ggacatgagg caaatggggg
     2041 gtggttgcgg gcggccggga tgccgggagg acgagagcgc gaagaacctc ggtcctccgt
     2101 tcggtcccgg tcgatcgcag ggtgtgtcag gccccgagga gctccatcat cttcgaggaa
     2161 ccgtccttgg agatccactc gacgttcttg aactgactga agtcgatgcc gtagagcagc
     2221 tccagcacgt catcgtatga ggttgcggcg ctgggaatga cgggtgcctt cttcgtcgag
     2281 aggtccacct tttcctcgat gggcgcgctg tccttcgtcc gggcggtcct gttctcgcgg
     2341 ggggaccgtg tctgtccgag cagggtgctg ccctgcgaaa cgtttcctgt tttcattgcc
     2401 acctcctgaa aaacatagcg aaatacatta tatcacaata gcggaattgt aaagaaaact
     2461 ttgtgatacc tcatgcggat gcaggcaggg cgaggcctgc cgacttcttc caggaccacg
     2521 ggcagtcgca cctgccatcg atgcagtaga gatggttgca gtaatcctga aactcgcacg
     2581 ccgcttggtc gataaaggat gcgacagcca tctcgtagag gccgacgaag aggtactggc
     2641 cgtcagcgaa gtgttccgcg gacgccgggg tgaaaggact cttttgcccc gtcctcggaa
     2701 gggtcatcag atgtgttgtg tccaggatct tccgtcccat gtttcctccg tacaccgcta
     2761 tagaatcgaa tcaggaacct tgaaagcaag aaccgtgcct ctgaccggaa ccagtgaatt
     2821 cggatagtta gtgcgaatgg atttgtcgcc tttggcccga aagcggtctt tgttgacgcc
     2881 ccggtgtccg atatctgaca tgcccggtct ccggcaggcg gcgttccttt cgcgtaacgt
     2941 gttgtaaccg ggagcggcgc gggaaggcac gacgatcagc atctcgtcga ctgctattgc
     3001 ggaagaatat tatccttgca aatcgaaacc tactctttta atatacaaga atgatagcga
     3061 gaaagataca ggcttcagtc gaagagtccc tgaagcaatt tcccgttgtg ggtcttatcg
     3121 gtccccgaca ggccggaaaa accactctgg ccaaaatgat cctccaaaaa acggaaacaa
     3181 ggccccttta ccttgatctg gagctttcct ccgacgtcaa taagcttcgc gatcccgaaa
     3241 tgtacctgcg aagatacgag gacaggctgg tcatcataga cgaagtccag cgcatgcccg
     3301 agcttttccc gctcatgagg gcgatggtag atcaaaagag aagcgccggc cggttcctta
     3361 tccttggttc ggcatcacca gcccttgtaa ggcaggcctc ggagacactc gcgggccgtg
     3421 ccgtatatcg cgagttgttg cctttctctc ttgacgagat cgggtgcggg aataccgacc
     3481 gactctggct tcgtggcggc tatcccttga gttatctcgc cgccggaaat gatgaaagct
     3541 attcatggcg cgaggcgttc atcaggacct atctcgaaat ggacataccc cagctcggga
     3601 taagggtgcc ggcattgcag ctcagacgat tctggaccat gctggcgcat agtcacggac
     3661 aattatggaa tgcatcaaag atcgcgggaa gcctgggaat ttccgctccc accgcaaagc
     3721 actaccttga cattctctca gacacctttg tcgtgcggca gctccagccc tttttcgcga
     3781 acgtcaaaaa acggttgata agatctccca aagtatactt gcgtgatgcc ggactgttcc
     3841 acacgttgat gcgcatcaag gaaaaagagg accttgacgc gcacccttcc gccggggcct
     3901 cctgggaagg gttcattatt gaacagatca ttgccgcaaa gccggacgac tgggaggctt
     3961 atttctatcg gacctccgca ggagcagaga tagatctttt gcttcttgat gaaagcggca
     4021 ggcccaccgc gatcgagatc aagtactcgt tgagtccaaa acccggcagg ggtttctgga
     4081 gcgcgatcga tgatcttgcc tgcgagaaag cgtttgtgat atttcccggc gatgaatcat
     4141 acccgctaaa ggaaaatgca ttcaccctgc ccgtggcgca gatacaaaga atatttcact
     4201 gaaacgaaca ccggtaacga aaaagccgct ctttcgagcg gctcatattt ttgaatggtg
     4261 gagctgaagg ggatcgaacc cttcaataca cactctaacc agtcgatata taaggctatt
     4321 acttatcctg ttttcttagt agggacactc tggggacagt ccctttcttc aaggtgaatt
     4381 gtttgagtac tcccctttgc gtttctaccg acaggtgagt gtagcgtttt gtcatctgat
     4441 cgtttgaatg t
//
LOCUS       DAOPOA010000101         4445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_21313_length_4445_cov_3.507289, whole genome shotgun sequence.
ACCESSION   DAOPOA010000101 DAOPOA010000000
VERSION     DAOPOA010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21313_length_4445_cov_3.507289"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /locus_tag="PKJ17_03240"
     CDS             complement(<1..1938)
                     /locus_tag="PKJ17_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOD75015.1"
                     /translation="MMERVCYHRETVESLCESLKSRSTGLGSDEAARRLDEYGPNELK
                     AKKKKTPVMMFLDQFKDFMILILLGAAVVAGVIGELTDTLIIIAIVIANAVIGFVQEF
                     RAEKAIEALKQMAAPMAAVLRDSNVRSIASRDIVPGDVVLLEAGVIVPADMRLIESAQ
                     MRVDEAALTGESVPVEKSVGELCDADVSIGDRVNMVYKGTIVTYGRGMGLVVGTGMET
                     ELGRIASMLQDEDEVRTPLQKRLTVFGKNLAIAILIICAVVFVIGLMRGERPLLMLLT
                     AISLAVAAIPEALPAVITISLALGAKKMVKKNALIRKLPAVETLGSVTYICSDKTGTL
                     TYNRMTVEELYARGEVVGSREDRARGLADLMRGIALNNDSREGTDGAFIGDPTEVALY
                     TAARDNGYERERIETDFPRVGEIPFDSDRKCMTTIHSVPGGGFVSYTKGAPDVLIERA
                     RTMWADGAQEPLNKEGILAANEDMAKRGLRVLCVAMRTWESLPGECSIDMIESDLTIL
                     GLVGMMDPPRDEARQAVDLCRTAGIRPVMITGDHPVTAAAIAKRIGILDETSDGVMTG
                     KELAALGDDVFASKVEHIQAYARVAPEQKLKIVKALQERHQFVAMTGDGVNDAPALKR
                     ADIGVAMGVTGTDVSKEAAHMI"
     gene            complement(2379..>4445)
                     /locus_tag="PKJ17_03245"
     CDS             complement(2379..>4445)
                     /locus_tag="PKJ17_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75016.1"
                     /translation="GYFNWTEPDGSTTQKYMYVVPLSQRTADGKRLSVFVTTYLDEFT
                     KPLREAQAIQQKTARNLISTTSYLLGSFRDNGLLFMGIGILLVSFVALAIGMYFSRGI
                     SRLSEATRRVNEGDFKVSVEPVFSGEIKTLMEDFNRMVARLDETTVSKGLLEESEKRL
                     LEANIDLRREIAVRSLAEKALAAEKERLAVTLRSIGDGVITVDTEGKVILINKAAETL
                     TGYSQQEADGRSFTEIFPLRDNGIDGADEDPVRALLKGTEPASLSTPAVLVGRDGTER
                     LVATTASLIRSEDATVIGAVIVFRDVTEQHRMEAELLKARKLESIGTLAGGIAHDFNN
                     LLAVILGNISFARMLLDDDQKAVKRLDEAEKATIRGKDLSYRLLTFARGGAPVKKLTV
                     LDDVIRDAAELTVSGSTSRCVYNFPEDLYRAQIDEGQIRQVVHNLVMNARESMPDGGT
                     ITIAAENVDLVHPEGTLTPGRYIRLTFQDKGCGISPEDLERVFDPYFTTKEMGSEKGM
                     GLGLAICYSIVRSHNGYISISSEPGAGTTVSVYVEAYEGDAESWPAPGVMRQGKGKKV
                     LYMDDEEQVRDLAGQILEHLGYNVEFARDGAEAIELFRNGVASGAPYDLVVLDLTVPG
                     GMGGREAMENILAINPMVKAVVSSGYVNDAVVRDYKKYGFSGVVAKPYSIEQFRRVIE
                     SVFNGK"
BASE COUNT          784 a         1343 c         1309 g         1009 t
ORIGIN      
        1 gatcatgtgg gcggcttcct tcgagacgtc cgtgccggtg acgcccatcg ccacgccgat
       61 atcggcccgc ttgagggcgg gcgcgtcgtt gacgccgtcg ccggtcatcg ccacgaactg
      121 gtgcctttcc tgcagggcct tgacgatctt cagcttctgc tcgggggcga cccgggcgta
      181 ggcctgaata tgttccacct ttgacgcgaa gacatcgtcg ccgagcgcgg caagttcctt
      241 tccggtcatg acgccgtcgc tcgtctcgtc gaggatgccg atgcgcttcg cgatggcggc
      301 ggccgtgacg ggatggtcgc cggttatcat gacgggcctg atgcccgcgg tcctgcaaag
      361 gtcgacggcc tgccttgctt catcgcgggg cgggtccatc attcccacaa ggccgagtat
      421 ggtgaggtcg ctctctatca tgtcgatgga gcattcgcca gggaggcttt cccaggtccg
      481 catggcgacg cacaggaccc gaagccccct ctttgccatg tcttcgttcg cggccagaat
      541 accttctttg ttcagaggct cctgtgctcc gtcggcccac atcgtccggg cccgttcgat
      601 gaggacatcg ggggcaccct tggtgtagga cacgaaaccg ccgcccggca cgctgtggat
      661 ggtggtcatg cacttgcgat cggaatcaaa ggggatctcg cccacgcggg ggaagtccgt
      721 ctcgatcctt tccctctcgt acccgttgtc gcgggccgcg gtgtagaggg cgacctccgt
      781 gggatcgcca atgaaggctc cgtcggtgcc ttccctggag tcgttgttca gggcgatgcc
      841 ccgcatgaga tccgcaagtc cgcgggccct gtcctccctg ctcccgacga cctctccccg
      901 ggcgtagagc tcttcgacgg tcatgcggtt gtacgtgagc gtccccgtct tgtcggagca
      961 gatgtacgtg acggacccga gcgtttccac cgcggggagc ttcctgataa gggcgttctt
     1021 tttcaccatc ttcttcgcgc ccagcgcgag ggagatggtg atgaccgccg gcagggcctc
     1081 tggtatggcc gcgacggcga gagaaatggc cgtcaggagc atgagcagcg gccgctctcc
     1141 ccgcatgagg ccgataacga agacgacggc gcatatgatg aggatggcta tggcaaggtt
     1201 ctttccgaag accgtcagcc gtttctgaag gggggttctg acctcgtcct catcctggag
     1261 catggaagct atccttccca gttccgtctc catgccggtg ccgacaacga gacccatgcc
     1321 tctgccgtag gtgacgatag ttcccttgta gaccatgttc accctgtcgc cgatggagac
     1381 atccgcgtca caaagctctc cgacggattt ctccacggga acggattccc ccgtgagggc
     1441 ggcctcgtcg acgcgcatct gggcgctttc gatgaggcgc atgtccgccg gaacgatgac
     1501 gcccgcttcg agaagaacga cgtcgcccgg aacgatgtcc cgcgaggcta tcgaccggac
     1561 gttgctgtct ctcaggacgg ccgccatggg agcggccatc tgtttcaagg cctcgatggc
     1621 cttttccgcc ctgaattcct ggacgaaacc gatgacggcg ttggcgatga cgatggctat
     1681 gatgatgagc gtgtccgtca gttccccgat gacgcccgcg acgacggcgg caccgaggag
     1741 gatgaggatc ataaagtcct tgaactggtc gaggaacatc atcaccggcg ttttcttctt
     1801 ctttgccttc agctcgttgg ggccatattc atcgagcctt ctcgcggcct cgtcgctccc
     1861 gaggcccgtg ctccggctct tcaagctttc gcaaagggat tccaccgttt ccctgtggta
     1921 acaaacacgc tccatcattc tctcctctcc tgtgatacga tcggccctgg tgcgggtcgg
     1981 tcgttctggc aaaggtctcg cttgttagaa tctctaaccg gcataccggt cggttttaag
     2041 gccgacgtat tgacgatggt gccgatgaag ctactcccct ttgatggcaa tccctgtcaa
     2101 cccatggagt attgtatacc tcacttcctc acgagctgca aagtcgggca tgtaccgccc
     2161 tccagatgtc tttcgcgccc gaagtggaca caaaaaaaga gacgctttaa catgaacata
     2221 accttcatgc caaaggtctc gctcgatcgg tcccttgcgg tgtgaccgaa gacagggcca
     2281 ggattctcac tccggattgt tgaccccgtc tcttatagct actccccttc agggctgggg
     2341 tattatactg cagtctgcgc aggaattcaa cggtcctgtc actttccgtt gaacacgctt
     2401 tcaatgaccc ttctgaattg ctctatggag taaggttttg ccacgacccc ggaaaagccg
     2461 tatttcttgt agtcgcgtac gacggcgtca ttcacgtatc cgctggacac gacggccttc
     2521 accatggggt tgatggcgag gatgttctcc atcgcctcgc ggccccccat gcccccgggc
     2581 acggtaaggt cgagcaccac aaggtcgtag ggggcacccg aggcaacccc gtttctgaag
     2641 agttctatcg cctcggcccc gtcccgggca aactcgacgt tatatcccag gtgttccagt
     2701 atctgtcccg ccaggtcccg gacctgttcc tcgtcatcca tgtagaggac cttctttcct
     2761 tttccctggc gcatgacacc gggggccggc catgattccg cgtccccttc ataggcctcg
     2821 acatagacgg acaccgttgt tccggctcct ggttcggaag agatggatat atagccgtta
     2881 tggctcctga ctatggaata gcagatcgcc agacctaatc ccattccctt ctcgctgccc
     2941 atttccttgg tcgtgaagta agggtcgaag acacgttcaa gatcctcggg ggagatgccg
     3001 catcccttgt cctgaaaggt cagacggata tatctgccgg gagtcagggt cccttcggga
     3061 tgaacgaggt ccacgttctc ggcggctatc gtgatcgtgc ccccgtcggg catggattcc
     3121 cgggcgttca tgaccagatt gtggacgacc tgcctgatct gtccttcgtc gatctgtgcc
     3181 ctgtacagat cttcgggaaa gttgtagacg catcttgaag tggagccgct cacggtaagt
     3241 tcggcggcat cgcgtataac gtcgtcgaga acggtgagct tcttcaccgg tgcgccgccc
     3301 cgggcgaagg tgaggagccg gtaggaaagg tccttacccc ggatcgttgc tttttccgcc
     3361 tcatccagcc tcttcactgc cttctgatcg tcgtcgagga gcatccgcgc gaaggatatg
     3421 tttcccagga tgactgcgag gaggttgttg aaatcgtggg cgatgccccc tgccagggtg
     3481 ccgatggact caagctttcg cgccttgagg agttcggctt ccatcctgtg ctgctcggta
     3541 acatcgcgga aaactatcac ggcgccgatg accgtggcat cttcgctgcg gatgagcgag
     3601 gccgttgtgg ccacgagccg ttccgtccca tcccgtccga caagaacggc gggtgtcgag
     3661 agagaggctg gctcggtgcc cttgaggagg gctctgactg ggtcctcgtc tgccccgtcg
     3721 atgccattgt ccctcagggg gaagatctcc gtgaaggacc tgccgtcggc ttcctgctgc
     3781 gagtagccgg tgagcgtttc agcggccttg ttgatgagta tgaccttgcc ttcggtatcg
     3841 acggttatga cgccgtcccc gatggacctg agcgtgacgg caaggcgctc cttctccgcg
     3901 gccagggcct tttccgccag ggagcggacg gcaatctccc gcctgaggtc gatgtttgcc
     3961 tcaaggagtc ttttttcgct ctcttcgagg agccccttgg agaccgtcgt ttcgtcgaga
     4021 cgagccacca tgcggttgaa atcctccatc agcgtcttga tctctcccga gaaaacgggc
     4081 tcgacggaca ccttgaagtc accttcattg accctcctgg tcgcttcgct caggcgcgag
     4141 atgccacgcg agaaatacat cccgatggcg agggcgacga aagagacaag gaggatccct
     4201 attcccatga ataggagtcc gttgtcacga aaagacccga ggagatacga tgtggtgctg
     4261 ataaggtttc tcgccgtctt ttgctgtatc gcctgggctt ccctcaaggg tttcgtgaac
     4321 tcgtccagat aggtggtcac gaacacgctg agacgtttgc cgtcagcggt gcgctgggac
     4381 aggggcacga catacatgta tttctgggtg gtggaaccgt caggttctgt ccagttgaaa
     4441 taccc
//
LOCUS       DAOPOA010000102         4440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_21340_length_4440_cov_3.836716, whole genome shotgun sequence.
ACCESSION   DAOPOA010000102 DAOPOA010000000
VERSION     DAOPOA010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21340_length_4440_cov_3.836716"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="PKJ17_03250"
     CDS             <1..1497
                     /locus_tag="PKJ17_03250"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOD75017.1"
                     /translation="VIEMVAGRILAPFVGVSIYTWTSIIGVILAGISIGAYIGGKLVD
                     RYPSRKTLGWLLFISGVMALTIIPLTYAIAAYRFPVSLMLRIFMVTSIIFFIPGCVLG
                     TISPVVVRLTLKNLENAGNVIGKIYAISTLGAIIGTFVTGFFLISWMGTRTIILSMGI
                     VLLVVALLAGSIFRKKTSMAIFVIIAVPCLFFINSYLYAIPASGKTYLYKESDYYTIK
                     LSKTMSSDRKTELEAMVLDNLIHSYVNLKDPKHIEYEYERIYADVLTWKFTEDTPFKS
                     LTIGGGGYTFPRYMETTYPRAKVDVVEIDPEVTKIVYDHLGLPKDTRIVSYNTDGRWF
                     VMNCKEKYDLVFTDAYNDISIPYHLTTKEFLRQIHDIMNPGAILMSNIIDNFQKGLFL
                     PSYIKTLRQVFGEKNVYLISVSPNFRKVRISTFIVIAGKGNFDIASFQSFIQHRLGSR
                     ATSSIVPEDLMDEFINRKDAIVIHDDHAPIDNLVAPVFEERFGYNRKN"
     gene            1691..1766
                     /locus_tag="PKJ17_03255"
     tRNA            1691..1766
                     /locus_tag="PKJ17_03255"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1724..1726,aa:Gly,seq:ccc)
     gene            2000..2731
                     /locus_tag="PKJ17_03260"
     CDS             2000..2731
                     /locus_tag="PKJ17_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75018.1"
                     /translation="MKGKDQAKGKQPGLKAFWIKAGLVVAVGLIAIFRPDVPPAVFTF
                     PLIGPVKVYHIFWALAVFTLVKRMIPRFNKKISSRKIFGRFYRQAENITPKRDEQLRS
                     LKARTDRGALKSAFYWLLLLGDMALWRMVGMLSDTWIYIIVLFFIFMDQFCVTVFCPF
                     KWLAGGKCCSTCRINNWGYLMAFSPLIFVQSFWTWSIVALSIIVAIQWEYLYHRFPER
                     FYETHNAALMCRNCTVECTGHRKLR"
     gene            2818..3741
                     /gene="trxB"
                     /locus_tag="PKJ17_03265"
     CDS             2818..3741
                     /gene="trxB"
                     /locus_tag="PKJ17_03265"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOD75019.1"
                     /translation="MSDQHEAIVIGGGPAGLTAGLYLMRGEIKAVLLEKVLPGGAPLN
                     TGRIENYPGFPEGISGRELMERFTTHAKAFGLPVREFAEVLSVTREEGLFTVTVNDGA
                     LTARGVIVASGTEPVRMGIPGENELVGRGISYCATCDGMFFRDLDVAVIGGGDSAIEE
                     ALSLANIVRKVYVVHRRDALRAQKILQERAFRNPKMEFLWNRRPVEIAGSQQVERLVI
                     EDTKTGRREDLPVSGVFVYVGSRANTGFLGDLVERDPAGFIITDEGLATATPGLYAAG
                     DIRKKTLRQVSTAVGDGATAAVGLERYILGA"
     gene            3788..4378
                     /locus_tag="PKJ17_03270"
     CDS             3788..4378
                     /locus_tag="PKJ17_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75020.1"
                     /translation="MGALLLCLAGLLLPAAVQPLRAAEHIVLSRAGAADDAMWNSLRK
                     YLAGKGFTMSVYDSPASMEKQVEIANKANKERATFMLAVELVPSDSTDAFVAVSNAKK
                     GKGLILNVDEVPGSHASRSGEMASSIAAAFQRKVKAIPLFMFLGIDMPGAFVRLEVQK
                     ARPADAFDKLHEGLLNYMKRGKDERERQGERRNTSS"
BASE COUNT          961 a         1313 c         1230 g          936 t
ORIGIN      
        1 gtgatcgaaa tggtcgcggg acgcatcctc gcgcctttcg tgggggtttc gatctacacg
       61 tggacgagca tcatcggcgt catccttgcc ggcatcagca tcggcgccta tatcgggggc
      121 aagctcgtcg accgctaccc ctccaggaag acgctgggat ggctcctttt catatcgggt
      181 gtgatggcgc tgacgatcat tcccctcacc tacgccatcg ccgcctaccg gttccccgtt
      241 tccctgatgc tgcggatctt catggtcaca tccatcatct tcttcatccc cggctgcgtc
      301 ctcggcacca tctcgcccgt cgtcgtgagg ctgaccctca agaacctcga gaacgcgggg
      361 aacgtcatcg ggaagatata cgccatctcg acgctggggg ccatcatcgg gacattcgtc
      421 acgggcttct tcctcatctc ctggatgggc acgcggacga tcatcctctc catgggcatc
      481 gtgctgctcg tcgtggccct tctggcggga tcgatcttcc ggaagaagac cagcatggcc
      541 atcttcgtca tcatcgcggt gccatgcctc ttcttcatca actcttacct ctacgccatc
      601 ccggcgagcg gcaagaccta cctttacaag gaaagtgact actacacgat caagctgtcg
      661 aagacgatga gctccgaccg taagacggag ctcgaggcga tggtcctcga caacctcata
      721 cactcctacg tgaacctcaa agacccgaaa cacatcgagt acgagtacga acgtatctat
      781 gccgacgtcc tgacgtggaa gttcaccgag gatacaccct tcaagagtct cacgatcgga
      841 ggaggcggat acaccttccc ccggtacatg gagaccacct accccagggc aaaggtcgac
      901 gttgtcgaga tagaccccga ggtgacgaag atcgtctatg accatctcgg tctgccgaaa
      961 gacacgagga tagtgagcta caacaccgac ggcaggtggt tcgtcatgaa ctgcaaggag
     1021 aagtacgacc tcgtcttcac cgatgcctac aacgacatct ccatacccta ccacctgacg
     1081 acgaaggaat tcctccggca gatccacgat atcatgaacc ccggggcgat cctgatgtcc
     1141 aacatcatcg acaactttca gaaggggctc ttcctgcctt cctacatcaa gaccttgaga
     1201 caggtcttcg gtgagaagaa cgtctatctc atctccgtga gccccaactt caggaaggtc
     1261 cggatcagca ctttcatcgt catcgccgga aaggggaatt tcgacatcgc cagcttccag
     1321 tccttcatcc agcaccgcct gggctcccgg gccacatcct ccatcgtgcc cgaagacctc
     1381 atggacgagt tcataaacag aaaagacgcc atcgtcatcc acgacgacca cgcccccatc
     1441 gacaacctcg tagcccccgt cttcgaggaa aggttcggat ataacagaaa gaactaggaa
     1501 agactgtttt aagttttaag ttttaggtaa aagacaaatg aaaagcggtg ggatgcgccg
     1561 gtatcccccg gaaacccccg ggttcttcct gcttttaacc taaaacctaa aacctaaaac
     1621 ttaaaacggg cttcaacacc tccttttcct ttgctttctt ccttcttttc ttttagaatt
     1681 atacttcggt gcgggcgtaa ctcagttggt agagtgcaag cttcccaagc ttgatgccgc
     1741 gggttcgagc cccgtcgccc gctccaattg aaaacaatgc ccaatttacc aataaccaat
     1801 tgaacaaaga gaggggcaca acggactgcc tgtatcgttt tcctggttct tggttattgt
     1861 ctgcttctct cgttattgga atttgtttat tggtcattat cgtttcgtcc ctcgttgaca
     1921 ttctgtcctt cctccagtta ttcttgcgga tgaaccgcgc ggaccccatt aaagcacgtg
     1981 cctgtctccg ggtagacctg tgaagggcaa ggatcaagcg aagggcaaac aaccggggct
     2041 caaggctttc tggataaagg ccggtctcgt cgtggcggtg ggtctcattg cgatcttccg
     2101 ccccgatgtc ccaccggccg tcttcacctt tcctctcatc ggtcccgtga aggtatatca
     2161 tatcttctgg gccctggccg tattcaccct cgtgaaaaga atgatccccc gtttcaacaa
     2221 aaagatctct tcgcggaaga tattcggccg gttctacagg caggcggaga acatcacacc
     2281 aaaaagggat gaacaactcc gctccctgaa ggcaaggacg gaccggggag ccctgaagtc
     2341 ggccttctac tggctgctgc ttctcggcga catggcactg tggcgcatgg tgggcatgct
     2401 gagcgacacc tggatctaca tcatcgttct cttcttcata ttcatggacc agttctgcgt
     2461 gaccgtcttc tgtcccttca agtggctcgc gggcggcaag tgctgcagca cctgccgcat
     2521 caacaactgg ggctacctca tggccttctc gcccctcatc ttcgtgcagt cgttctggac
     2581 gtggtccatc gtcgcccttt ccatcatcgt cgccattcaa tgggagtatc tctatcaccg
     2641 tttccccgag cggttctatg aaacacacaa tgccgccctg atgtgcagga actgcaccgt
     2701 ggagtgcacg gggcacagaa aattacgttg aatattgcgg ggggattcct tacaatgaga
     2761 gacacggcag gcggcctcgc aggaggtcgc cgcggtcctt tcgggaggtt ttcgaatatg
     2821 tcggatcagc acgaagcgat agtcatcggc ggcgggcccg cggggctcac ggccggtctc
     2881 tatcttatgc gaggcgagat aaaggccgtc cttctcgaga aggtccttcc cggcggggcg
     2941 cctctcaaca cggggcgcat cgagaactat ccggggtttc ccgaaggcat atccggcagg
     3001 gagttgatgg aaaggttcac cacccacgcg aaggccttcg gccttcccgt cagggagttc
     3061 gcggaggtcc tcagcgtcac gcgcgaggag ggactcttca ccgtgacggt gaacgacgga
     3121 gccctcacgg cccggggcgt catagtggcc agcggcaccg aaccggtcag gatggggatc
     3181 cccggcgaga atgagctcgt gggcaggggc atctcatatt gcgccacctg cgacggcatg
     3241 ttcttcaggg acctcgatgt ggccgtcata ggcggcggcg attcggccat cgaagaggcc
     3301 ctgtcgctcg ccaacatagt gaggaaggtg tacgtggtcc atcgcaggga cgcgttgagg
     3361 gcgcagaaga tactccagga gcgcgccttc aggaacccga agatggaatt cctctggaac
     3421 aggcgccccg tcgagatcgc cggcagtcag caggtggaac gccttgtcat cgaggatacg
     3481 aagacgggcc gccgtgaaga tctaccggta agcggggtct tcgtctacgt cggcagccgg
     3541 gccaataccg gctttctcgg cgaccttgtc gaacgcgacc cggccggctt catcatcacc
     3601 gacgaagggc tcgccacagc gacaccggga ttgtatgcgg caggagacat ccggaaaaaa
     3661 acgctccggc aggtctccac ggctgtcggc gacggggcga ccgcggctgt cggcctcgaa
     3721 aggtatatcc ttggcgcata gcggtaaacc cttcaattcg ctgaggccgc gaaggtggtc
     3781 gtccttgatg ggcgcgctgc ttttgtgtct cgcaggcctc cttctccctg cggccgtgca
     3841 acccctccgc gccgcggagc acattgtcct ttccagagcg ggcgccgcgg atgacgccat
     3901 gtggaacagc ctcaggaaat accttgccgg caaggggttc accatgagcg tctacgatag
     3961 ccccgcctca atggagaaac aggtcgagat cgcgaacaaa gcgaacaagg aaagggcgac
     4021 cttcatgctc gccgtcgagc tcgttccgtc ggacagcacg gacgccttcg tcgccgtgag
     4081 caacgcgaaa aaagggaagg gtctgatatt gaacgtggat gaggtgccgg ggtcgcacgc
     4141 ctcccggtcg ggagagatgg cgtcgtccat tgcggcggcg ttccagagga aggtgaaggc
     4201 gatccccctt ttcatgttcc tggggataga tatgccgggg gccttcgtga ggctcgaggt
     4261 tcagaaggcc cggcccgcag acgccttcga caaactgcat gaaggtttgc tcaattatat
     4321 gaaaagaggt aaggatgaga gagaacgcca gggcgagcga cgaaatacgt catcttaaaa
     4381 tagaaaaaag cttcctcagg tatcccgagg gttccgtgct cgtcgaaatg ggggcaacga
//
LOCUS       DAOPOA010000103         4398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_21628_length_4398_cov_2.530279, whole genome shotgun sequence.
ACCESSION   DAOPOA010000103 DAOPOA010000000
VERSION     DAOPOA010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21628_length_4398_cov_2.530279"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..843)
                     /locus_tag="PKJ17_03275"
     CDS             complement(277..843)
                     /locus_tag="PKJ17_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOD75021.1"
                     /translation="MKRIVVGMTGASGVIYGIRLLDTLRSHGAEPHLVISGAARKNIL
                     IETAFTVEQVEKMAHRVYDPDDLAAPISSGSFRTGGMVIAPCTVKTLSGVANSYTENL
                     IVRTADVTLKEKRKLVLVVRETPLHRGHLELMVKAAGLGAVILPPMPAFYHRPKSLDD
                     IIDHTVGKILDMLDIEHDLFRRWGTETD"
     gene            complement(840..1265)
                     /locus_tag="PKJ17_03280"
     CDS             complement(840..1265)
                     /locus_tag="PKJ17_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75022.1"
                     /translation="MDSEAHPGSARIQGINVNAFTLRQAIGPFAVEGHVVSMGKDLLV
                     ALCGGEGHIGAAGMADVRPSMRHPGRLSSTSSVFTYPGHKEDVVVKEISEFLSKEMDR
                     RVVVIAGLHWEDLNDDEIAAIIEACRALAREIVKRKGDI"
     gene            complement(1270..2154)
                     /locus_tag="PKJ17_03285"
     CDS             complement(1270..2154)
                     /locus_tag="PKJ17_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOD75023.1"
                     /translation="MEPIRTIRAKILHFTELVKFEHSVFALPYVLMSLFLVSNGSLDM
                     RVTLWVIACMVSARSAAMSFNRIADYHYDRKNVRTSGRPLQTGKVRLVEAWVFTVAMA
                     SVFVFSSWMLNMPSFMLSFVALSVLFGYSFTKRLTWLSHWVLGVCLGLGPVGVWVAVR
                     ADIEAVGILLGTGVALWVAGFDIIYATQDLDFDRKEGLKSIPSRFGIGKALIISLLCH
                     VAAVGTYLAAGIASGLGPYYYTGLFCIAICLFYEHVILKKHGLSRVNEAFFTVNGAVS
                     VLFFIFSVIDILFYRTSP"
     gene            complement(2133..3569)
                     /locus_tag="PKJ17_03290"
     CDS             complement(2133..3569)
                     /locus_tag="PKJ17_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOD75024.1"
                     /translation="MDLKKLVATWEEKGLLKRIGQPVSAELEVTEIVRRVFEKKGPAL
                     LFENVKNAGMPLLTNIFGTWQRVWDIFGVDSEDALISRVKPLLEIEPPKGLVDKARAL
                     WKIKGVTGNLPKEVGKARCQSRVLTGDGINLADLPVMKCWPDDAGRFITLPVVVTKDP
                     EKGVQNFGMYRMQVIDERTTAMHWHPGKGADIHYQRSKKLGKPLEAACVLGCEPAVIY
                     SATAPLPPDINELILAGMLMDKPVETVRCRTVDLTVPANSQIVLEGYVEGSETFLEGP
                     FGDHTGYDTPQRPFPVFHITALTTDNDPIYPSTVVGWPPFEDAILGTLTERIFLPFIK
                     FIIPDIKDMALPAAGLFHNFVFASIDKRYPGQAYKAMDSIWGLGQLSTSKVVVIFDSH
                     VDIHRQDEVLFHLGNNIDPLRDVVIKKGPLDILDHASSEEGFGGKMGIDATGKLPEEG
                     HKRPWPARALVDDETKKNVDALWSRLGL"
     gene            complement(3747..>4398)
                     /locus_tag="PKJ17_03295"
     CDS             complement(3747..>4398)
                     /locus_tag="PKJ17_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75025.1"
                     /translation="TKQGPSVIGIVMATMLEAAPFITGLSLVKEKDGPFPVYGNEQVR
                     LVISGIGKTCSAMATTYFIQACQPSCICNIGASGSTSGRHLPGEIYHIAKIIEPDRLD
                     LKAGTPHEHTPDILPGFPLATLSTRDSPLRDPGEREGAAAYAQLADMEGASVAQVCRH
                     FNVRCFLFKFVSDTPEHTLNSDIVRNIESHRDACAHFFRHSILPGLIAAKAAATQQ"
BASE COUNT          898 a         1262 c         1229 g         1009 t
ORIGIN      
        1 ctcacccgtg ccaacctcac cgcagccaac ctcaccgcag ccaacctcac ccgtgccaaa
       61 ctcaccggag ccgatctcac cggagccgat ctcaccggag ccgacctcac caacgtcaaa
      121 ctcacagggg tcatcctcac cggcaccaaa ctctgatctc tgattacagc gggtggtgcg
      181 tgtcgacata acgatcattc cagtgacacg tcgatgtcat gccatgtgtc cggtcctgtc
      241 ggcatgctct tggggtccat gtgcacaact gcgggatcag tcggtttccg tcccccatct
      301 cctgaagagg tcatgctcga tatccagcat atcaaggatc tttcccacgg tgtggtcgat
      361 aatgtcgtca agggattttg gcctgtggta aaaggccggc atcggaggaa gtatgacggc
      421 gccgagaccg gccgctttca ccataagttc gaggtgcccc ctgtgtagcg gggtctccct
      481 gacgacgagg acgagctttc ttttttcttt taaggtgaca tcggctgtcc tgacgatcag
      541 gttctcagtg tatgaatttg ctacaccgga cagcgtcttg acggtgcatg gcgctattac
      601 catgccgcca gtcctgaagg aaccgctcga tatcggagcg gccaggtcgt ccgggtcgta
      661 gacccggtgg gccatctttt ccacctgttc cacggtgaat gccgtttcga tcaggatgtt
      721 cttccttgcg gcgccggaaa tcacaaggtg gggctcggcc ccgtgcgacc tcagtgtgtc
      781 caggaggcgg ataccgtata tcacaccgct cgcacccgtc atacccacta cgatgcgctt
      841 catatgtcac ccttcctttt cacgatctct ctggcaaggg cccggcatgc ctctattatc
      901 gcggctatct catcatcgtt gaggtcttcc cagtggagcc cggcgataac aaccaccctg
      961 cggtccattt ctttcgaaag gaactccgag atctccttga cgaccacatc ttctttatgt
     1021 cccggatagg tgaagaccga gcttgtcgaa ctgagcctgc ccggatgccg catgctcggg
     1081 cgcacatccg ccatgcctgc cgcacctatg tgcccttcgc cgccacagag cgcaacaaga
     1141 aggtcttttc ccatggacac cacgtgccct tcgactgcga atgggccaat tgcctgtcgt
     1201 aacgtaaaag cgttcacgtt tatcccctgt atacgggcgc tgccgggatg ggcctcgctg
     1261 tccattgtgt catggggacg tcctgtagaa caagatgtca ataacggaaa aaatgaagaa
     1321 aaggacgctt actgccccat tcacggtaaa gaaggcttcg ttgacgcggg aaaggccgtg
     1381 ttttttcagt atcacgtgct cgtagaaaag acatattgcg atacagaaaa ggcctgtata
     1441 gtaataaggg cccaggccgc ttgcgatgcc ggcggcaagg tacgtgccga ctgcggcaac
     1501 gtggcacaat agcgagatga tgagcgcttt gccgatcccg aacctggacg gaattgactt
     1561 gagaccttct ttcctgtcga agtccagatc ctgggtggcg tatatgatgt caaacccggc
     1621 tacccacagg gccacgcccg ttccgagaag gatgccgacc gcttcgatat ccgcacggac
     1681 ggcaacccag accccgaccg gtcctaggcc caggcagacc cccagcaccc agtgcgaaag
     1741 ccatgtcagg cgtttcgtga aagaatagcc gaagagtacc gacagggcca cgaaagagag
     1801 catgaacgag ggcatattga gcatccatga tgaaaagacg aacaccgagg ccatggcgac
     1861 cgtgaaaacc cacgcttcga caagcctgac cttgccggtt tggagagggc gcccgcttgt
     1921 cctcacattt ttcctgtcgt aatggtaatc ggcgatcctg ttgaaggaca tggccgcgct
     1981 tcgcgcgctc accatgcagg cgatgaccca cagggtgaca cgcatgtcca agctgccgtt
     2041 ggagacgagg aaaagcgaca tcaacacgta gggcagcgcg aatacgctgt gctcgaactt
     2101 gacgagttcc gtaaaatgaa gtatcttcgc ccttatagtc ctaatcggct ccataatgcg
     2161 tccacgttct tttttgtctc gtcgtccacc agcgctctcg ccggccacgg ccgtttatgg
     2221 ccttcttcgg gcagttttcc ggtcgcgtca atccccatct tgccgccaaa gccttcttcg
     2281 gacgaagcat ggtcgagaat gtcaagcggc ccctttttga tcacaacgtc cctgagaggg
     2341 tctatattgt tgcccagatg gaacagtact tcatcctgcc ggtgaatgtc gacgtggctg
     2401 tcgaatataa cgaccacctt agaggtggag agttgcccca agccccatat ggaatccata
     2461 gctttgtagg cctggcccgg gtatcgcttg tctatcgatg caaaaacgaa attgtgaaaa
     2521 aggccggctg cgggcagggc catatccttt atgtcgggga tgatgaactt gatgaagggg
     2581 aggaatatcc tttccgtgag tgttcccaag atcgcatcct cgaaaggcgg ccacccgacg
     2641 acggtcgagg ggtagatcgg gtcattgtcg gtggtgaggg cggtgatatg gaaaacgggg
     2701 aatggcctct gcggcgtgtc gtaacctgtg tggtcaccga agggcccctc gaggaatgtc
     2761 tccgatccct ccacgtaccc ttcgagtact atctggctat ttgcggggac ggtcaggtcc
     2821 acggtcctgc atcgcaccgt ttccaccggc ttgtccataa gcatcccggc aaggatcagc
     2881 tcattgatgt cgggggggag cggagcggtg gcggaataga taacggcagg ctcgcatccg
     2941 agcacacagg ccgcttcaag gggcttgccg agcttcttcg atctctggta atggatgtcg
     3001 gcgccctttc cagggtgcca gtgcattgcg gtcgtcctct cgtcgatcac ctgcatcctg
     3061 tacatcccga aattctgaac gcccttttcg gggtcttttg tgacaactac cggcagggtt
     3121 atgaacctgc ccgcatcatc tggccagcat ttcatgaccg gaaggtctgc cagatttatc
     3181 ccgtctcccg taagaacgcg actctggcac cgggccttgc cgacctcttt cggcaggttt
     3241 cccgtgaccc cttttatctt ccacagggcc cgggccttgt caacgaggcc cttcggaggt
     3301 tcgatctcca ggaggggctt tacgcgtgat atcagggcat cctccgaatc aaccccgaag
     3361 atgtcccata cgcgttgcca cgtgccgaat atattggtaa gcaggggcat tcctgcattc
     3421 ttcacgttct cgaagagcaa tgcagggcct tttttttcga aaacacggcg tactatttcg
     3481 gtaacctcga gctcggcgct taccggctga ccgatcctct tgagaaggcc cttttcttcc
     3541 catgtggcga caagtttttt caggtccatt acgcatgccc tccggtcgtt cgatccttgt
     3601 acccccgcgt gtttttgcct ttgtcaaccg ggcgaagggt aaaagtgcct tgtgaaggaa
     3661 aaatacaata gcatattttt gtggccattt catgcttttc gtcctgtgtg gggtagccct
     3721 tttccggccg atcgcgtttc gttcttctat tgctgtgttg ctgccgcttt agcggcgatc
     3781 aggccgggaa gtatggagtg acggaagaaa tgtgcgcagg cgtcacgatg tgactcgata
     3841 tttcttacaa tgtccgagtt cagtgtatgc tccggggtgt cggaaacgaa cttgaataga
     3901 aagcacctga cgttgaaatg ccggcatacc tgtgccactg acgccccttc catatcggca
     3961 agctgggcgt acgccgcggc gccctctctt tccccggggt cgcgcagcgg gctgtcccgg
     4021 gtggacaggg tggcgagagg gaatcctggc aatatgtcgg gtgtgtgttc gtgcggggtg
     4081 ccggccttca ggtccagccg gtccggttcg atgatctttg caatatggta gatctctccc
     4141 ggcaagtgcc gcccgctggt ggagcccgag gcgccgatgt tgcatatgca ggaaggctgg
     4201 caggcctgaa tgaaatatgt ggttgccatg gctgaacacg tcttcccgat ccccgagatg
     4261 acgaggcgga cctgctcgtt gccgtagacg gggaaggggc cgtccttttc cttgacgagg
     4321 gaaaggcctg tgatgaaggg ggccgcctcc agcatggtcg ccatgacgat cccgatcaca
     4381 gaaggcccct gtttcgtg
//
LOCUS       DAOPOA010000104         4354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_21925_length_4354_cov_3.667132, whole genome shotgun sequence.
ACCESSION   DAOPOA010000104 DAOPOA010000000
VERSION     DAOPOA010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21925_length_4354_cov_3.667132"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKJ17_03300"
     CDS             complement(<1..233)
                     /locus_tag="PKJ17_03300"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOD75026.1"
                     /translation="MIDLTGMNITMASGKGGTGKTTVAVNFAWLLASAGQRVQYLDCD
                     VEEPNGHIFLKPSFTESSPSKVMVPVVDQEKCTS"
     gene            complement(501..1265)
                     /locus_tag="PKJ17_03305"
     CDS             complement(501..1265)
                     /locus_tag="PKJ17_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOD75027.1"
                     /translation="MPRPTCKRRIGFQPKAVFFKPAGIPLGVVQEIVIGHDEVEAMRL
                     KNLLGYPQEEAANQMGVSQPTFHRLINAAHQKITDAIINGKALRIDGGNVTINEETVG
                     PCHWRKHWGRGCSSAEAVASQVSLTQEERGGIPMKVAITSIDGTLDGMVDERFGRAKK
                     IVVVDDGTNSQEAVDNVTNMNAAQGAGIQTAQNVIQAGAKAVISGHLGPNAFRVLSTA
                     GVEVYTASGMTVRDALEAYKAGKLPKLTGADVEGHW"
     gene            complement(1427..1714)
                     /locus_tag="PKJ17_03310"
     CDS             complement(1427..1714)
                     /locus_tag="PKJ17_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75028.1"
                     /translation="MADKSPPSILASLRKTAGEFLYGMTVHELDLEIRKDKGHLNDLF
                     MLIVFGDLVGLPILPPFYAMRILPHIIPHLERWKKGICRERDITEIFTGDL"
     gene            complement(1745..2224)
                     /locus_tag="PKJ17_03315"
     CDS             complement(1745..2224)
                     /locus_tag="PKJ17_03315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOD75029.1"
                     /translation="MEEKAFQDYYAEDVSHCYGCGRFNEEGLHIKSYWDGETSVARFT
                     PRPCHMAAPGFVYGGVIASLVDCHGTGTAAAAAYRFEGRAMDTEPNLRFVTASLKVDY
                     LLPTPLGPELVARGTVKSVGKRKVVVAVEVLAEGELCARGEVVAVKMPGAMKKTRGN"
     gene            complement(2372..2590)
                     /locus_tag="PKJ17_03320"
     CDS             complement(2372..2590)
                     /locus_tag="PKJ17_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOD75030.1"
                     /translation="MSSIVDAKGLSCPQPVILTMNEIKKTGKGEITVLVDTVTSRENV
                     SRAAMAQGWTLKSVEEEGEVFRITITKD"
     gene            complement(2610..3704)
                     /gene="yedE"
                     /locus_tag="PKJ17_03325"
     CDS             complement(2610..3704)
                     /gene="yedE"
                     /locus_tag="PKJ17_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HOD75031.1"
                     /translation="MSRLVQIFATRWGIIAVGAFIGVFAQLLQKWGNPANMGICVACF
                     ERDIAGALGLHRAAVVQYMRPEIIGFVLGSLIAAYAFKEFRARAGSAPIVRFILGAFA
                     MIGALVFLGCPWRAALRLAGGDWNAIFGLLGLIGGVWVGTLFFRGGYNLGRSQTTHTS
                     VGWLLPLTMLGFLALMLVFPQVPQQDKSGVLLYSVKGPGSMHAPLFLSLAIGLVIGFL
                     AQRSRFCTMGAIRDLVLFRQTHLLSGFVSLVVFAFLTNLALGQFHPGFANQPVAHTMT
                     LWNFGGMVLAGLAFALAGGCPGRQLFLAGEGDGDAAVFVLGMIVGAGFSHNFGLASSP
                     QGVGPHGIAAVITGLLVCLFIGFTMRQKTA"
     gene            3997..>4354
                     /locus_tag="PKJ17_03330"
     CDS             3997..>4354
                     /locus_tag="PKJ17_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOD75032.1"
                     /translation="MENEYILTEIGNDHICRITLNRPENLNTFNTPMAMELDRSLRSA
                     DKDENIRVIVLKGVGKAFCAGIDVSEFPGKTAMEYSEWIGLMGAPMVTMNDLRKPVIA
                     QVRGIAAAIGAGLVAAA"
BASE COUNT          919 a         1264 c         1215 g          956 t
ORIGIN      
        1 gatgtgcact tttcctgatc cacgacgggg accatgacct tcgatggaga tgattcggtg
       61 aaggaaggct tgagaaagat gtgaccgttg ggctcttcaa catcgcaatc aagatattgc
      121 acgcgctggc cggccgaggc gagcaaccag gcaaaattga ccgccacggt cgtcttgccc
      181 gttcctccct ttccgctggc catggtaatg ttcatacctg taagatcgat catgacgctc
      241 tcaccatctt ggagccgcac tgagggcaga cctgttcata acagggtact ccgcgtgtgt
      301 ggggcgatct cgtgccgcat tgcgggcact gacattcgcc gccgaccccg aggccgcggc
      361 ctcccatccg gcctcttccc atgcctgact gaccggcacc gcggcctgtg ccctgaccca
      421 gaccaccttt tccggtcatt ggcccttttc ctgtcggacc cgttccatct tttcgtggca
      481 tgatgtatac ctcctttgct ttaccagtgc ccctcgacat ccgctccggt aagctttggc
      541 aacttcccgg ccttgtatgc ttcgagtgcg tcccggacgg tcatccctga ggctgtgtat
      601 acctcgacgc ctgccgtcga aagcacacgg aaggcgttcg gcccgagatg gccgcttatg
      661 accgccttgg ctcccgcctg aatgacgttc tgggctgtct ggatgccggc gccctgggcg
      721 gcgttcatgt tcgtcacgtt atcgacggct tcctgactgt tcgttccatc gtccacgacg
      781 acgatcttct ttgccctgcc gaaacgttca tccaccattc catcaagcgt cccgtcgatg
      841 cttgttatgg ctaccttcat gggaatacct cctcgctctt cttgtgtcag ggaaacctgg
      901 gatgccacgg cttccgccga agagcatccc cgtccccagt gctttctcca atggcacgga
      961 ccgaccgtct cttcattgat cgtcacgttg ccgccgtcta tcctgagggc cttgccgttt
     1021 attatcgcgt cggttatctt ctgatgcgcg gcgttgatca agcggtggaa ggtcggctgc
     1081 gaaacgccca tctggtttgc agcctcctcc tgggggtatc ccaggaggtt cttgagccgc
     1141 atggcctcca cctcgtcgtg accgatcacg atctcctgga ccactcccag aggtatgccg
     1201 gcgggtttga aaaataccgc tttcggttgg aaccctattc gtctcttgca ggttggtctc
     1261 ggcatcctta cctcatgtca ttggtctcgt gtttcacata atgaatatat attcagaata
     1321 gtgtgcttgt caagaggaac gaagaagaaa gtttaaaagt tcaagggttc aaaagttcaa
     1381 ggaaaaaggc gacgccgggg ttccgcggtt caagacgaaa ccaattctag aggtcgccgg
     1441 tgaagatctc ggtaatgtcc cgttcgcggc agatgccttt cttccacctc tcgagatggg
     1501 ggatgatgtg gggaaggatc cgcatggcgt agaatggggg gagtatggga agaccgacaa
     1561 ggtcgccgaa gacgatgagc atgaagagat cgttcagatg gcctttgtcc tttcgtattt
     1621 ccaggtccag ttcgtggacg gtcatgccat aaagaaactc accggccgtt ttgcgaagag
     1681 atgcgagaat cgatggaggg ctcttgtctg ccatatgaaa tgtccgtcca cgggacggag
     1741 atgactaatt cccccgtgtt tttttcatgg ctcccggcat tttgaccgcg acgacctcac
     1801 cgcgggcgca aagttctccc tcggcaagaa cctcgactgc cactaccacc ttacgttttc
     1861 cgacgctttt gactgtgccg cgagccacca attcgggtcc cagaggtgtg ggcaggagat
     1921 aatccacttt gagggaagcc gtgacgaatc ggaggttggg ctccgtgtcc atggcccttc
     1981 cttcgaagcg ataggccgca gccgccgcgg tacctgtgcc gtggcaatcg acgagtgacg
     2041 cgatcacacc gccgtacacg aaaccgggtg ccgccatgtg gcagggcctt ggggtgaaac
     2101 gggctaccga tgtttctcca tcccagtagc tcttgatgtg aaggccttcc tcgttgaagc
     2161 ggccacaccc gtagcaatga ctcacatctt ccgcgtagta atcctgaaat gccttttctt
     2221 ccatcggacc atccttcctg aacccgctaa ttgaaacgga gcacaggatt atatgtatca
     2281 ttttgccgga agaaatgcat ccccgtattt ggaaaaaggc ggtgagaccc gcgccgggag
     2341 ccaaacggct ggatctcacc atccttaccc gttagtcttt cgtgatggta atgcggaaga
     2401 cctctccctc ttcctcaacg ctcttgagag tccatccctg agccattgcg gcgcgggata
     2461 cgttctccct ggaggtgacg gtgtccacga ggacggttat ctcgcccttg ccggtttttt
     2521 tgatctcatt catggtcaag atgaccggct gcgggcacga aaggcctttt gcatcgacga
     2581 tagagctcat tcttgatcct ccttgctgtt caggccgtct tctgtctcat ggtgaacccg
     2641 atgaagagac agaccaggag gcctgtgatc acggcggcga taccgtgagg gcccacaccc
     2701 tgaggggagc ttgccaggcc gaagttgtgg gagaagccgg ccccgacgat catccccagg
     2761 acaaagacgg cggcatctcc gtcaccttcg cccgcaagaa agagctgtcg tcccgggcac
     2821 cctccggcca gggcgaatgc cagtccggcc aggaccatgc cgccgaaatt ccaaagcgtc
     2881 atcgtgtggg ccacaggttg gttcgcaaag ccggggtgga actgtccgag tgccagattc
     2941 gtcagaaagg cgaacacgac gagtgagacg aacccggata aaagatgggt ctgtctaaaa
     3001 agcacaagat ccctgatggc gcccatggtg cagaacctgc tcctctgggc caggaaccct
     3061 atgacgaggc ctatcgccag ggacaggaaa agtggagcgt gcatcgagcc gggacccttc
     3121 acgctgtaaa gcagaacgcc gctcttgtcc tgttgaggga cctggggaaa gaccagcatg
     3181 agggcgagaa aacccagcat tgtcaggggc agcagccagc cgaccgacgt gtgggtggtt
     3241 tgcgagcgcc cgaggttgta gccccccctg aagaagagag tgcccaccca tacgcccccg
     3301 atgagcccga gaagtccgaa tatcgcgttc caatcgcccc cggccaaacg cagcgccgcc
     3361 ctccacgggc atcccaggaa gacgagcgcc ccgatcatgg caaaggcccc gagaatgaac
     3421 cggacaatgg gggcggaccc cgcccgcgcc cggaattcct tgaaggcata ggcggcgatc
     3481 agcgagccca ggacaaatcc gatgatctcg ggccgcatgt actggacgac agccgcgcgg
     3541 tgaagcccca gggctcccgc tatgtccctc tcgaaacagg cgacgcagat gcccatgttt
     3601 gccggattcc cccatttctg caagagctgt gcgaaaacgc cgataaaagc tcccaccgcg
     3661 attattcccc accgcgtcgc gaagatctgt acaagtcttg acataagctt atcccctgta
     3721 ttctgtttag ttgttctcag tacagaacca tagcaataac taatttgtca aattaatatt
     3781 atcaataact agtttcgctg tcattcgggc tccctatcgg tttatccggt gctgtctctg
     3841 ttccttcaag ggcttatcgg ggccacgtcg tccggatcac atccgttttg acaacgggac
     3901 gcgcaatttg atataggact aaagaaaccg tgattgagag acggctcaga tcacgttcga
     3961 tggaaaaatg ctgagaacat cagaaaggag cgcgttatgg agaacgaata cattcttacg
     4021 gagatcggca atgaccatat ctgccggatc acgctgaacc ggcctgagaa cctgaacacc
     4081 ttcaataccc ccatggcgat ggagctcgac cgttcccttc ggtccgcgga caaggacgag
     4141 aatatccgcg ttatcgtgct gaagggtgtg ggcaaggcat tctgtgccgg gatcgatgtc
     4201 agcgaatttc ccggaaagac cgccatggaa tacagcgaat ggataggtct catgggtgct
     4261 ccgatggtga ctatgaacga tctcagaaaa ccggtgatcg cccaggtgcg ggggattgcc
     4321 gcggccatcg gggcggggct cgtggcggcc gcgg
//
LOCUS       DAOPOA010000105         4318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_22162_length_4318_cov_3.357026, whole genome shotgun sequence.
ACCESSION   DAOPOA010000105 DAOPOA010000000
VERSION     DAOPOA010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22162_length_4318_cov_3.357026"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKJ17_03335"
     CDS             <1..614
                     /locus_tag="PKJ17_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOD75033.1"
                     /translation="DHAGEKAFCDGVDVADHTPDKVLDMIEVFHRMFRLMEELDCTTV
                     ALVNGRSLGGGCELMAFCDIVIASEKAKIGQPEIAVGVFPPIAAAWFPKIIGLKNTYE
                     LLLTGKIIGAKEAQAMGLVNAVLPVENFKAEADKFMADFLKQSRPVAMWTKRAIKAGL
                     NVNFLEALRASEICYLDGCMKTKDAVEGISAFMDKRKPVWKDE"
     gene            624..1796
                     /locus_tag="PKJ17_03340"
     CDS             624..1796
                     /locus_tag="PKJ17_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOD75034.1"
                     /translation="MKYAPKEEIEARITKLKTLMEKASLDGAFFHYKIDYYYLSGTMQ
                     DSLLFVPLDSDPVLFVKREISRARRESPLRNIMSYRSIKDIPQHLKPMRRLGMQLDVI
                     PYNDAIRFKDLFSDVEFVDCSPLIKELRKQKSPFEIGLMEKAAEIGRKVYAKVPEFLR
                     EGATEIEVGGQMEAYAKSLGHEGLLRVRSLNYEAYTWHILSGRTGTIVSQSDSPMGGL
                     GLSPAFPVGASFKRIRRNEPILVDFGICYHGYQVDQTRMYAVGSMPDMFVRGYDACRE
                     IHDRVLDKVVEGYTSKELFEYSKELGDKLGYGDYYLGYKPHKVRFLGHGIGIELAELP
                     YIAATHTYPIEDGAVFAIEPKMVFPKKGCCGYENTIHYTNGKCRIITDTDDSIIIA"
     gene            1793..2590
                     /locus_tag="PKJ17_03345"
     CDS             1793..2590
                     /locus_tag="PKJ17_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOD75035.1"
                     /translation="MKYLFSTGCLYHLPIEEIFVLAREGGFDGCELVIDQRFMSDGYL
                     DRVQACLDILPVFTIHAPFLKMNKWGTKVDELKRTIDIARVLGAGVVNFHPPAWLTME
                     IGFARWLRSIHDFQKELDCADIALTIENMPRAGKHLMLTSYILNDINDLVAFGQEHNL
                     YFTFDTTHLASFGVDIIAPFIMVFRTGRLRNIHLSDSGNHESHLFLGRGDLPIARFLN
                     TVRRLGYDGTITFEVSPRELPRSREWLVRLMEYQLRLIKLHTGASVV"
     gene            2592..3932
                     /gene="radA"
                     /locus_tag="PKJ17_03350"
     CDS             2592..3932
                     /gene="radA"
                     /locus_tag="PKJ17_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOD75036.1"
                     /translation="MAKRTVFVCSSCGHETLKWLGKCPMCQGWDTFKEFAPAPKTDGR
                     EPRRPVILTDEELADEERIATGIGEMDRVLGGGMTIGSAILLGGDPGIGKTTLCFEVA
                     AKLTELGLKVLYVSGEESLKQLASRKKRLGLKAGFAFLTTNHLDDILTAINEERYALV
                     IVDSVQAVYNPKLPMLPGSMGQVKDVSTRLTMEMKALDTSLIFIGHVTKEGVIAGPKV
                     LEHMVDTVLYFEGDKMLPYRMLRTIKNRYGAVEEVGIFQMTGDGLISVENPSQFFMSQ
                     RGDIGEGSSLFPCITGSRPIVLEVQAITQKTNFSMPRRLSLGYDVNRLFILISVIEKT
                     VGKPFFDRDIYVNVTGGMKVNEPAIDLPVAASILSSYKDAALGRETAFFGEVGLTGEI
                     RKTVNMDLRIKECQRIGIKRVFCPADGARSDGIEIVSIGNLKDLSQKIRKKAGE"
     gene            complement(4080..>4318)
                     /locus_tag="PKJ17_03355"
     CDS             complement(4080..>4318)
                     /locus_tag="PKJ17_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD75037.1"
                     /translation="KILKNVDIDVKKAVRTGVAFVEIIDYAKAEGIDLIVMGTHGRSG
                     IEHILIGNVAEKVVRKSPCPVMTIRPKGKGFKMP"
BASE COUNT         1013 a         1216 c         1228 g          861 t
ORIGIN      
        1 tcgatcacgc cggagaaaag gcgttctgcg acggcgtcga cgtggccgac cacaccccgg
       61 acaaggtcct cgacatgatc gaggtcttcc accggatgtt ccggctcatg gaagagttgg
      121 actgcacgac ggtggcgctg gtgaacggca gatcgctcgg cggcggatgc gagctcatgg
      181 ccttctgtga catcgtcatc gcttcagaga aagcgaagat cggccagccc gagatcgcgg
      241 tgggcgtgtt ccctccgatc gcggcggcat ggttcccgaa gatcatcggc ctcaagaaca
      301 cctatgagct tctcctgaca ggcaagatca tcggcgcgaa agaggcgcag gcaatgggtc
      361 tcgtcaacgc tgtccttccc gtggagaact tcaaggcgga agcggataag ttcatggccg
      421 atttcctcaa gcagtcgagg ccggtggcca tgtggacgaa gcgcgccatc aaggcgggtc
      481 tcaacgtcaa tttcctcgag gccctgaggg cgtccgagat atgctacctc gacggctgca
      541 tgaagacgaa ggatgccgtc gaagggataa gcgccttcat ggacaagaga aaaccggtgt
      601 ggaaggacga gtaaccgggc cagatgaaat acgcgccgaa agaagaaata gaagcacgga
      661 tcacaaagct gaaaacgtta atggaaaagg cctctctgga tggggccttt tttcattaca
      721 aaattgacta ttactacctc tcgggcacga tgcaggactc cctgctcttc gtccccctcg
      781 actccgaccc ggtgctcttc gtcaagcgcg agatatcgag agcgcgtagg gagtctcccc
      841 tccggaacat tatgtcctat cggtccatca aggacatccc gcaacacctc aagcccatga
      901 ggcgactggg catgcagctt gacgtcatac cttacaacga cgcgatcagg ttcaaggacc
      961 tcttcagcga cgtggaattc gtcgactgct cgccgctcat aaaggaactg agaaagcaga
     1021 aatctccttt tgagataggc ctcatggaga aggccgcaga gataggcagg aaggtatatg
     1081 cgaaggtgcc ggagttcctt cgggaggggg caacggagat cgaggtgggc ggccagatgg
     1141 aggcctacgc gaagtcgctg ggccacgaag ggctcctgag ggtccggtcc cttaactacg
     1201 aggcctacac ctggcacatc ctgagcggac gcacgggcac catagtgagc cagtccgatt
     1261 cacccatggg cggcctcggc ctgtccccgg catttcccgt cggcgccagt ttcaaaagga
     1321 taaggaggaa cgagcccatc ctcgttgatt tcgggatctg ctatcacggc taccaggtag
     1381 accagaccag aatgtatgcc gtcggttcga tgccggacat gttcgtcagg ggctacgatg
     1441 cctgcaggga gatccatgac cgcgtgctcg acaaggtcgt cgaaggatac acgagcaagg
     1501 agcttttcga gtactccaaa gaactggggg acaagctggg atacggtgac tactatctcg
     1561 ggtacaagcc gcacaaggtg cggttcctgg gtcatgggat cggcatcgaa ctggcggagc
     1621 ttccttacat agccgccacc catacctatc ccatagagga cggggccgtc ttcgccatcg
     1681 agccgaaaat ggtcttcccg aagaaaggtt gctgcggata tgagaacacc atacactaca
     1741 cgaacggaaa gtgccggata ataaccgata ctgatgacag catcattatc gcatgaaata
     1801 ccttttttcc acaggatgcc tgtaccacct ccccattgaa gagatcttcg tcctcgcgcg
     1861 cgaaggcggg ttcgacggct gtgaactcgt catagaccag cgctttatga gcgacggcta
     1921 tctggacagg gttcaggcgt gcctagacat ccttcccgtt ttcaccatcc atgccccctt
     1981 tctcaaaatg aacaaatggg gcacaaaggt ggacgaactg aagaggacca tcgatatagc
     2041 ccgcgtcctc ggagcgggcg ttgtcaattt ccatcccccc gcgtggctga cgatggagat
     2101 cggcttcgcc cggtggctga gaagcattca cgacttccag aaggagctcg actgcgccga
     2161 tatcgccctt accatcgaga acatgccacg ggcaggcaag cacctgatgc tgacgtcgta
     2221 catcctcaac gatatcaatg acctcgtcgc cttcggccag gagcataacc tctatttcac
     2281 ctttgatacg acccatctcg ccagttttgg cgtggatatc atcgcccctt tcataatggt
     2341 cttcaggacg ggcaggctgc gaaacattca cctcagcgac tccggcaacc acgaaagcca
     2401 tctcttcctg ggaagggggg acctccccat tgccagattc ctgaacacgg tccgaaggct
     2461 tggctatgac gggacaataa cgttcgaggt gtccccccgg gagcttccca gatccaggga
     2521 gtggctcgtc aggctcatgg agtatcagct tcgcttgatc aagctgcata caggggcttc
     2581 ggtggtgtga tatggcaaaa aggaccgtct tcgtctgctc ttcctgcgga catgaaacgt
     2641 tgaaatggct ggggaaatgc cccatgtgtc agggatggga cacgttcaag gaatttgcgc
     2701 ccgcaccaaa gacggacggc agggaaccgc gccggcccgt catcctcaca gatgaagaac
     2761 tcgccgatga agagaggatc gcgacgggca taggagagat ggaccgcgtc ctcggcgggg
     2821 gtatgacgat agggtctgcc atcctcctcg gcggcgatcc cggaataggc aagacgacac
     2881 tctgtttcga ggttgccgcg aagcttacgg aactggggct caaggtcctc tatgtctccg
     2941 gggaggaatc cctgaaacag ctcgcatccc gcaagaagag gctggggctc aaggcgggat
     3001 ttgcctttct caccaccaat cacctggacg acatcctgac ggcgatcaac gaggaacgtt
     3061 acgcccttgt catcgtggat tccgtccagg cggtctacaa ccccaagctg cccatgctac
     3121 cgggaagcat gggccaggtc aaggatgtat ccacgcggct gacgatggaa atgaaggccc
     3181 tcgatacgag ccttattttc ataggtcacg tcacgaagga aggtgtcata gcaggcccca
     3241 aggtgctgga acatatggtc gatacggtcc tgtacttcga gggcgacaag atgctcccct
     3301 accgcatgct caggaccatc aagaacaggt acggcgccgt cgaagaggtg ggtatattcc
     3361 agatgaccgg ggatggcctt atcagcgtgg agaacccttc ccagttcttc atgtcccagc
     3421 ggggcgatat cggcgagggg agcagtcttt tcccctgtat cacgggatcg cggcccatcg
     3481 tcctcgaggt acaggccata acccagaaga ccaacttctc catgccgaga aggctttccc
     3541 tcggctacga cgtgaaccgc cttttcatcc tcatctccgt catcgagaag accgtgggga
     3601 agcctttctt cgacagggac atctacgtca acgtaacggg aggcatgaag gtaaacgagc
     3661 ctgccattga cctgcccgtg gccgcgtcga tactctcaag ctacaaggat gccgctctcg
     3721 gccgggagac agcctttttc ggcgaggtcg ggctcaccgg cgagatcagg aaaacggtga
     3781 acatggacct caggatcaag gaatgccagc gcatcggcat aaaaagggtc ttctgccccg
     3841 ccgacggcgc ccgctccgac ggaatagaga tagtaagtat cggaaatcta aaggacttat
     3901 ctcagaagat acggaagaag gcaggggaat aggacctata ggagaagata ggacgtataa
     3961 gacctctaga acccatcaag agagttcttc gagtcttaca gccggatggc catcacggga
     4021 ccacgaacaa actggtttct tctaaatatt caagcggttc aagcggttca agccgtattt
     4081 tacggcattt tgaacccttt ccccttcggc ctgatggtca tcacgggaca gggggacttc
     4141 cgtaccacct tctccgccac gttccctatg aggatatgct ctataccgct gcgcccgtgc
     4201 gtgcccatga cgatgaggtc tataccctcg gccttcgcgt agtcgatgat ctcgacgaag
     4261 gccacgccgg tccttacggc cttcttcaca tcgatgtcga catttttcag gattttcc
//
LOCUS       DAOPOA010000106         4280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_22398_length_4280_cov_3.471953, whole genome shotgun sequence.
ACCESSION   DAOPOA010000106 DAOPOA010000000
VERSION     DAOPOA010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22398_length_4280_cov_3.471953"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..266)
                     /locus_tag="PKJ17_03360"
     CDS             complement(132..266)
                     /locus_tag="PKJ17_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75038.1"
                     /translation="MKAERLREIVRALMSSRFYFDLDIRERHGLVVHVLRMMEARRAS
                     "
     gene            464..913
                     /locus_tag="PKJ17_03365"
     CDS             464..913
                     /locus_tag="PKJ17_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75039.1"
                     /translation="MPVFDENGNQINTEEEFPKMKDPEEGLPPPAKKPFPILPAVLGI
                     IALVLAVVAVVFFLKANTLEQEVEILKKAKTQLATTEAKLQDAMKENQKVKAELGQAK
                     AELDSARAKNQALEDQLAKKKAPPATKKSPSQTDKKSVPKKPAPRKP"
     gene            933..1547
                     /locus_tag="PKJ17_03370"
     CDS             933..1547
                     /locus_tag="PKJ17_03370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD75040.1"
                     /translation="MKKELLILSTLVFLAMTCLVSFAEEGNKEKKMKQSYPPLSAVIK
                     TAKGDINVKLFPDQAPLTVLSFVNLSKRGFYDNLTFHRVIRGFMIQGGCPLGTGTGDP
                     GYRFKDEFSPELRHNKPGVLSMANAGPNTNGSQFFITHVPTPHLDDRHSIFGEVKGPK
                     DQEIVDSIAVGDRINTIVIEGDYSRLAEDHKEDLQKWNGILDKK"
     gene            1759..2301
                     /locus_tag="PKJ17_03375"
     CDS             1759..2301
                     /locus_tag="PKJ17_03375"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HOD75041.1"
                     /translation="MYIASKMFLTKGVGQNKEKLVSFELALRNAKIAPFNIVKVSSIF
                     PPQCEIVPAKQGLKRLTPGQIVHAVLAEASTNEHHRLVAASVGVAIPKDRDRFGYLSE
                     YHSHGEDEKTAGDYAEDIAAQMLATILGLEFDVNESYNKRLDVWKLSDEIVQTRNISQ
                     TSVGKKGVWTTVVAAAILIP"
     gene            complement(2371..>4280)
                     /locus_tag="PKJ17_03380"
     CDS             complement(2371..>4280)
                     /locus_tag="PKJ17_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75042.1"
                     /translation="RSLPDGGHREFSVNGTTGIQMDVVAYRMPVPDTGYSLVHLCPAE
                     KIYGTLASRDRIATISLLVIALLAGVAFTVRSMMLKTSVDGLERRVKERTADLYRLNE
                     SLQQEISERELAMRALEKAERKYRSIFENAVEGMFQTTPDGRIISVNPSLASMHGYGS
                     PREMVRELAGMAKRLYVDEGRRAEFVRLMEEDGAALHFEAQTHRKEGTMNWVSINARN
                     VRDVKGKTLCYEGSVENITERKHLESQLLHSQKMEAIGTLAGGIAHDFNNILTAIVGY
                     GNLLQLKMEKDDPLRTYTDRILSSSQKAINLTRSLLAFGRKQPVELRPHRVGTLIEGS
                     GKLLKRLLTEDIALRMGRVDGDAVIMADRTQMDQVLMNLATNARDAMPEGGTLAVEAE
                     RVVMDNDFVRTHGYGSPGDYVLIRVADTGAGMDEGTMKKVFEPFFTTKAEGKGTGLGL
                     SIVYGIVKQHNGYITIDSEPGGGTTFNMYFPEAKTGETVDSEAAQVPLNIRVGSETIL
                     VAEDNAEARSFLTEVLSVSGYTVIEAVDGDEALRAFSDNKDRIALVVSDVVMPGRNGG
                     ELREEIQKIKPDMKILFTSGYARDVLLFKGIHDKAANYISKPLSPNDLLGKVREMLDG
                     HPAHPEGPTGS"
BASE COUNT          954 a         1244 c         1168 g          914 t
ORIGIN      
        1 acaagggtga tggccttctt ccatgacaga acgctgatcg gttcaaaggt cgtgttaagc
       61 aggagggtcc ggtccattta atcatttgaa aaataacccg tttcaacctc tcatgtcaag
      121 atttgagatg cctacgaagc ccgtctggct tccatcatcc tcaggacatg caccaccagg
      181 ccgtgcctct cccggatatc gaggtcgaaa tagaaccggc tgctcatgag cgcccttacg
      241 atctctctca gtctctcggc cttcaccaca tactcctgaa aaggattatc ggacgaaagg
      301 gcaaaaacta taaagttgcg ggttgcgggt tgccagagag acaatcttta acgcgcaacg
      361 cgcaacgcgc aacgcgcaac cgcctttgcc tttttctctg gtctttcgct atgttctgtg
      421 gtaagtatgg acaaaatacg taaagcaaaa ggagccagcg aaaatgcccg tgttcgatga
      481 gaatggaaat cagataaaca ccgaagaaga attccccaaa atgaaggatc ccgaggaagg
      541 attgcctccc cccgcgaaga aaccgtttcc cattcttccg gccgtcctgg gaataatcgc
      601 cctcgtcctc gctgtcgttg ccgtcgtttt cttcctgaag gcgaacaccc tggagcagga
      661 ggtggagatc ctgaagaagg ccaagaccca gctggcaaca acggaggcca aactccagga
      721 cgcgatgaaa gagaaccaaa aggtaaaggc tgaattgggc caggccaagg cggagctgga
      781 cagcgcaagg gcgaagaacc aggccctgga ggaccagctg gccaagaaga aggctccccc
      841 cgcgacgaag aaatctccct ctcagacgga caagaagtct gttccgaaga aacctgctcc
      901 caggaaaccg taacgataaa gaggtcgcag ccatgaagaa agagctattg atcctatcaa
      961 cactggtctt tctggccatg acgtgtctcg tctcgtttgc cgaagaagga aacaaggaga
     1021 agaaaatgaa acaatcatat ccaccgttaa gtgccgtaat aaagacggca aaaggcgaca
     1081 taaacgtaaa gcttttcccc gatcaggcac ccctcacggt gttgagcttc gtgaacctgt
     1141 ccaaacgggg cttttacgac aacctgacgt tccacagggt gatccgaggg ttcatgatcc
     1201 agggtggctg ccccctggga acgggcacgg gggaccccgg ctatcgtttt aaagatgaat
     1261 tctcgccgga gctgagacac aacaagccgg gggtcctgtc catggcgaac gcagggccga
     1321 acacgaacgg aagccagttc ttcatcaccc acgtcccgac accgcatctc gatgacaggc
     1381 actcgatatt cggcgaggtc aaaggcccca aggaccagga gatcgtcgac agcatagccg
     1441 tgggggacag gataaacacg atcgtcatcg aaggcgatta ctcccgtctc gccgaggacc
     1501 acaaggaaga cctgcagaaa tggaacggga tcctcgataa gaaataggcc ttgccccatc
     1561 tgcacaatat tgtgaaatat tgtgagagtt gccttgtgcg gagggcggta tcgtggtatg
     1621 ctgacgaccg taaagcgtaa ggcgtagggc acaagtcacc ggagcatctg atcggtcgac
     1681 gattgccttt caccccttac gccttgtgtt tcaggcctca cggcactgtc aagtcttttg
     1741 ggaaaggaga ttcagcacat gtatattgcc agcaagatgt tccttacaaa gggggtgggc
     1801 cagaacaagg aaaaactggt gagctttgag ttggccctcc gcaacgccaa gatagccccc
     1861 ttcaacatcg tcaaggtatc gagcatcttt cccccacaat gcgagatcgt gccggcaaag
     1921 caggggctca agcggctgac tcccggccag atagtgcacg ccgttctggc tgaggcctcc
     1981 accaatgagc atcatcgcct ggttgcggcc tccgtcggcg tagccattcc caaggaccgt
     2041 gaccgtttcg gatatctttc cgaataccac agccatggag aggacgagaa aacggctggt
     2101 gactacgccg aagatatcgc cgcccagatg ctggcaacca tcctgggcct cgaattcgac
     2161 gttaatgaaa gctacaacaa gcggctcgac gtgtggaagc tgagcgacga gatagttcag
     2221 acacgcaaca tcagccagac aagcgtcggc aagaaaggtg tctggacaac ggtggtcgcc
     2281 gcagccatcc ttatacccta agaaaccgtt ccgggttgcg cgttgcgggt tccgggttaa
     2341 taaacctgta gacaccgaaa aaggggagtg ctaactccct gttggtcctt ccggatgggc
     2401 ggggtgtccg tccagcattt ccctgacctt tcccagcaag tcattgggtg atagcggctt
     2461 tgaaatgtag ttggctgcct tgtcgtgtat tcccttgaag agcaggacat cccgggcgta
     2521 tccgctggtg aagaggatct tcatgtccgg cttgatcttc tgtatctcct cgcgaagctc
     2581 tccgccgttc ctgccgggca tgacgacatc ggaaacgaca agcgcgatcc tgtccttgtt
     2641 gtcgctgaat gcgcgaaggg cctcatcccc atccacggcc tctatgacgg tatatccgga
     2701 gacactgagg acctcagtca ggaagctccg cgcttccgcg ttgtcctcgg cgacaaggat
     2761 ggtctcgctc cccacgcgta tgttcagggg aacctgcgcc gcctcgctgt ccacggtctc
     2821 cccggtcttt gcctccggaa agtacatgtt gaatgtcgtt cccccgccag gttcgctgtc
     2881 gatggtgata taaccgttgt gctgtttgac tatgccatag acgatggaca gacccagacc
     2941 cgttcccttg ccttccgcct tggtggtgaa gaacggctcg aacaccttct tcatcgtccc
     3001 ttcgtccatg ccggctcccg tgtctgcaac acggatgagc acgtagtctc cgggggaacc
     3061 gtatccgtga gtgcgcacga aatcattatc catcaccact ctctcggcct cgacggcaag
     3121 cgtccctcct tcgggcatgg catcgcgtgc atttgtggcc aggttcatca gcacctggtc
     3181 catctgtgtt ctgtcggcca tgatcaccgc gtcaccgtca acgcggccca tcctgagcgc
     3241 tatgtcttcc gtgaggagtc ttttcagaag ctttcccgac ccttctatca gggtgcccac
     3301 cctgtgcggc cttagttcca ccggctgctt cctgccgaag gcaagcaggc tccgggtgag
     3361 gttgatcgcc ttctgcgatg aggagagtat cctgtccgtg tacgtcctca ggggatcgtc
     3421 cttttccatc ttgagctgga gaaggttgcc gtagccgacg atggccgtca ggatgttgtt
     3481 gaagtcgtgg gcgatcccgc cggcgagggt gcctatggcc tccatcttct gtgagtgcag
     3541 aagctgtgat tcgagatgct tccgttcggt gatgttttcg acggacccct cgtaacagag
     3601 tgtcttaccc ttcacgtccc gcacattcct ggcattgatg gagacccagt tcatggtccc
     3661 ttccttccgg tgggtctgcg cctcgaagtg cagcgcggcc ccgtcttcct ccatcaaccg
     3721 gacgaattcc gcccggcgcc cttcatcaac atagagccgc tttgccatac cggcgagttc
     3781 ccggaccatc tcccgcggtg agccgtatcc gtgcattgag gccagagagg ggttgacgct
     3841 gatgatgcgt ccgtccggcg tcgtctggaa catcccttcc acagcgttct caaagatgga
     3901 gcggtacttc ctttccgcct tctccagcgc ccgcatggcc agctcccgtt cggatatctc
     3961 ctgctggagg ctctcattga ggcggtagag atcggcggtc cgttccttga cccgcctctc
     4021 gagtccgtcg acactcgtct tgagcatcat ggacctcacg gtgaaggcga cccccgccaa
     4081 aagagctatc acgagcaggg atatggtggc gatacggtcg cggctcgcca gcgttccgta
     4141 gatcttttcc gcgggacaca gatggacgag cgaatagcct gtgtcgggga caggcattct
     4201 gtaggcgacc acgtccatct gaatgcccgt cgtcccattc acggaaaact cccggtgtcc
     4261 cccgtcaggc agcgacctca
//
LOCUS       DAOPOA010000107         4235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_22697_length_4235_cov_3.838278, whole genome shotgun sequence.
ACCESSION   DAOPOA010000107 DAOPOA010000000
VERSION     DAOPOA010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22697_length_4235_cov_3.838278"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2680)
                     /locus_tag="PKJ17_03385"
     CDS             complement(<1..2680)
                     /locus_tag="PKJ17_03385"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOD75043.1"
                     /translation="MSGGGSGSRRILAWGFLFTLLFAAIYAFMPNLFRQVDLMNYDLL
                     LRHFPDNHESGRVVIVDLDERTLKRYGQWPWPRYRLADLLDRIAAAEPAVVALDMVFA
                     EPDRTSARRVLDDLSAFHGREVAIGRLPVDLSDNDGVLAETLTRGPFVLGNQFHFGNS
                     GNSSEQCVLHPLKASFLRSGGRQEENRGIPEGAGLLCSLPMFSEKAAATGFLNFSPDG
                     DGVARRLQLLIRYRGKVYPSLALATVLKLKGTDSILLKEDGKSLQSLRFGTTSVAVDD
                     RGQLLVRFRGPKRTHAYVSAADVMDGGVPSGKLRGKVVLVGTSAAGMAEWLATPFGPT
                     FPGIETHAAVVDNLLTGDLVAEPGWSRGLVLLLVIIPGAALSLLFRFRGTAFCLVATA
                     LFLAALWLTMQQVFFHAGLFVGTTFPMASIVCGCAVLAALKHREEQRRAEDSLRESEA
                     RFRTLFAMAPISLANIARDGRVLEVNNSLTRVMGYTADDLPTVERVWDLCMSDPSIRD
                     GFAAQWRAALADSTARDPATESLECPVLCKDGTSRTMVIATESVRGGVIVAFFDITER
                     KRAEEDRQRLERQLLQARKMDAIGQLAGGVAHDFNNMLNVIIGNAEMALGKAAPGEAL
                     REELQDILTAGRRSADLTRQLLAFARKQIVSPRVLNLNDAVAGMLRMLRRLIGENINL
                     AWRPEQGLWLVRMDPSQVDQLLANLTVNARDAMNKTGKIIIETSNAICDELFQAAHPE
                     SAPGEYVLLTVTDDGCGMDKETLANIFEPFFTTKEEGMGTGLGLSTVYGIVRQNGGFI
                     DVYSEPGRGTTFRIYLPRYGADVRESVDDRAITKMQGGSETVLVVEDEQSVLNLTRAM
                     LEKLGYRVLAVKGAVHALRLAREYDGDIDLLLTDVVM"
     gene            complement(2757..3359)
                     /locus_tag="PKJ17_03390"
     CDS             complement(2757..3359)
                     /locus_tag="PKJ17_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD75044.1"
                     /translation="MNKLVIFLAVLSVFALLVGCAARSTVVLIPDPDGNVGQVIVANE
                     QGQQVLNEANQSVIVAGRNAAPGAVKTMTTDEIRSVFSDALAARPLPPARFILYFLMD
                     SDELTRESKAVVPEVLATIRKRSSVDIVISGHTDTVGTNDYNYRLGLDRTKAIYDILV
                     AKGVAASSITATSHGEGNPLIKTGDNIPEPRNRRVEIAVK"
     gene            complement(3426..3890)
                     /locus_tag="PKJ17_03395"
     CDS             complement(3426..3890)
                     /locus_tag="PKJ17_03395"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOD75045.1"
                     /translation="MRDINVIQKLCLLCLMMTVLSPCLVMGEGSSIGKARTGKGDIVV
                     VRAGQKILLKVGDRLYQKDTIRTGKDSAVGIIFEDNTVLSLGPNSEMVIDEYIFAPEK
                     GMLSMVARILKGTASYLSGIIGRQSPESVKFHTPDATIGIRGTQFLVKAEGG"
     gene            4035..>4235
                     /locus_tag="PKJ17_03400"
     CDS             4035..>4235
                     /locus_tag="PKJ17_03400"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOD75046.1"
                     /translation="MSYKFDSLLIILNRIDSGKRVTADSLMEELEVSKRTVHRYITTL
                     QTANFPIEYDRERRTYAFTGGYT"
BASE COUNT          861 a         1316 c         1198 g          860 t
ORIGIN      
        1 tcatcaccac gtcggtcagc aggaggtcta tgtcgccgtc atactctctg gccaggcgga
       61 gcgcatggac ggctccttta accgcgagaa ccctgtagcc cagtttctcg agcatcgccc
      121 tggtgaggtt caataccgac tgctcgtcct ccaccaccag gaccgtctcg ctgcccccct
      181 gcatcttcgt tatcgccctg tcatccacgg attcccggac atcggccccg tagcggggca
      241 gatagatcct gaatgtcgtg ccccggcccg gttcgctgta tacatcgatg aaaccgccgt
      301 tctgcctgac aatcccgtac accgtcgaca gaccgaggcc cgtccccata ccctcttcct
      361 tggtggtgaa gaagggttcg aagatgttgg ccagcgtttc cttgtccatg ccgcagccgt
      421 cgtcagtcac tgtgagcagg acgtactcac cgggagcgga ctcggggtgg gccgcctgaa
      481 acagttcatc acagatcgcg ttagacgtct cgataatgat ctttcccgtc ttgttcatgg
      541 catcgcgggc gttcaccgtc agattggcaa ggagctggtc cacctgggag gggtccattc
      601 tgacaagcca cagtccctgc tccggccgcc acgccagatt gatgttctcg cctatgagcc
      661 ggcggagcat tctgagcatt ccagccaccg cgtcattgag gttgagaacc ctggggctga
      721 cgatctgctt gcgggcaaac gccagcaact ggcgcgtcag gtccgcggac cgtctccccg
      781 cggtaaggat atcctgaagc tcctcgcgga gggcctcgcc gggagccgct ttgccgagcg
      841 ccatttctgc gttcccgatg atcacattga gcatgttgtt gaaatcgtgg gcgactccac
      901 ccgccagctg tccgatggcg tccatcttcc gggcctgaag caactggcgt tccagcctct
      961 gacgatcttc ctcggccctc ttgcgctcgg tgatgtcaaa aaaagccacg atgacgccgc
     1021 ctctcaccga ttccgtcgcg atgaccatgg tgcgcgacgt cccatccttg cacagcaccg
     1081 ggcactccaa ggactccgtg gccggatccc ttgccgtcga gtcggcgagc gccgcgcgcc
     1141 attgtgccgc aaacccgtcc ctgatgctgg ggtcggacat acaaaggtcc cacacgcgct
     1201 ccacggtggg gaggtcatcc gcggtgtacc ccattacccg cgtgaggctg ttgttcacct
     1261 ccagaaccct tccgtcccgg gctatgtttg ccagggaaat gggggccatg gcaaagaggg
     1321 tgcgaaaacg cgcctcgctc tccctcaggg agtcttccgc gcgtctctgc tcctcgcggt
     1381 gcttgagcgc ggcaaggacc gcgcagccgc agacgatgga cgccatgggg aatgtcgttc
     1441 ccacgaacag gccggcgtga aagaagacct gctgcattgt caaccacagg gcggcaagga
     1501 agagcgccgt ggcgacaagg cagaaggcgg tacccctgaa gcggaagagc aggctcagtg
     1561 ccgcgcccgg aatgatgacg agaaggagga cgagacctct cgaccaaccg ggctcggcaa
     1621 cgagatcgcc cgtcagcaga ttgtcgacca cggcggcgtg ggtctctatt ccgggaaagg
     1681 tggggccgaa aggcgtcgcc agccactcgg ccatgcccgc ggctgaggtg ccgacgagca
     1741 caacctttcc ccgcagtttc cccgatggaa cgccgccgtc catgacatcc gccgccgaca
     1801 cgtaggcgtg ggtccgcttc gggccccgga acctgacgag caattggccg cgatcgtcga
     1861 cggctacaga cgttgtcccg aaacgcagtg actgcaggct cttcccatcc tctttaagaa
     1921 ggatgctgtc tgtccctttc agtttcagga ccgtcgccag ggccaggctg gggtagactt
     1981 ttcccctgta ccggatgagc agctgcaggc gccttgccac accgtcgccg tcgggactga
     2041 aattcagaaa acctgtcgcg gcggcttttt ccgagaacat gggcagactg cacaggagac
     2101 cggcaccctc gggtatcccc cggttctcct cctgcctccc accgctgcgc agaaaggatg
     2161 ctttcagcgg atgcagaacg cattgttccg agctgttccc ggagttgccg aaatggaact
     2221 gattgcccag gacaaaaggt ccccgtgtca gtgtttccgc caggacgccg tcgttatcgc
     2281 tcaggtccac agggagacgg ccaatggcca cttcacgtcc gtggaaggcg ctgagatcgt
     2341 caagcaccct tctcgcggac gtcctgtcag gttcggcaaa gaccatgtcg agagctacga
     2401 ccgctggctc cgcggcagct atcctgtcga gcagatcggc caaccggtac cgcggccagg
     2461 gccattgacc gtatcgcttg agcgtcctct cgtcaagatc gacaatcacc accctgccgc
     2521 tttcgtgatt gtcggggaaa tgcctgagaa gcaggtcgta attcatgaga tcgacctgcc
     2581 ggaacaggtt gggcataaac gcgtagatgg cggcgaagag cagggtgaac agaaatcccc
     2641 aggcaaggat gcgccggctc cccgacccgc cgcccgacat gggcatcctg aaatcgtctg
     2701 ctggacgttt acccgatctc attttcatca atgacttcgc cgtgcgatat cagaagctac
     2761 tttaccgcta tctctactct ccggttcctc ggttccggaa tgttgtcgcc cgtcttgatg
     2821 agagggtttc cttcaccatg cgaggtggcg gtgatgcttg aagcggccac acctttcgcc
     2881 accaggatgt cgtagatggc ttttgtccgg tcaagaccga gcctgtaatt gtaatcattt
     2941 gtgccgaccg tgtccgtgtg tcccgatatg actatgtcga cagaactcct cttccggatg
     3001 gtcgcgagga cctcgggtac gactgccttc gattcacggg tcagctcgtc tgaatccatc
     3061 aggaaataga ggatgaaccg cgccggcggc agcggccggg ctgccagagc atcggaaaaa
     3121 acggagcgga tctcgtcggt cgtcatggtc ttcaccgcgc cgggcgctgc attcctgccc
     3181 gccacgatca ccgactgatt cgcctcgtta agaacctgct gtccctgctc gttcgccacg
     3241 atcacctgtc ccacattgcc atcgggatca gggatcaaca cgaccgtact tctggcagcg
     3301 caacccacca gaagcgcgaa aacactcaag actgcaagga agataacgag cttgttcacg
     3361 ttcccccgcc agataggacg gcacatcgcc agggttcacc gggctgcggt tcggaagctc
     3421 accccttatc cgccctccgc cttgaccagg aactgtgttc cacggatacc gatcgtggca
     3481 tcgggagtgt ggaactttac cgactccggt gactgcctgc ctatgatgcc tgacagatac
     3541 gaggcggtgc ctttcaggat acgggcaacc atggacagca ttcccttttc gggggcgaag
     3601 atgtattcgt cgatgaccat ttcgctgttc ggccccagcg acaggaccgt attgtcctcg
     3661 aaaatgatcc cgacggcgct gtccttcccc gtccgtatgg tatccttctg gtaaagccgg
     3721 tcgccgacct tgaggagaat cttctggccg gctcgcacga caacgatatc gccttttcct
     3781 gtcctggctt tccctatcga agaaccttct cccatgacga ggcacgggga gaggaccgtc
     3841 atcatgagac agaggagaca cagtttttgt ataacgttga tgtctctcat tgtattaata
     3901 taccgacaca acaataaaaa atacaagcat ttacttgccg aagcccgggg ttaggaataa
     3961 aaacatggtc tcagatagtg acattccgct gtcactcggc tattataaga tggggatggc
     4021 gaaagggggc atgcatgtcg tacaaattcg attcactatt gatcatcctg aacaggatag
     4081 acagcgggaa acgggtcacg gccgattcac tcatggaaga gctggaagtg agcaagcgaa
     4141 ccgtgcacag gtacatcacc actctgcaaa cagcgaactt ccccatcgag tatgaccgcg
     4201 agagaaggac ctacgccttc accggcggat acacc
//
LOCUS       DAOPOA010000108         4224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_22758_length_4224_cov_4.637563, whole genome shotgun sequence.
ACCESSION   DAOPOA010000108 DAOPOA010000000
VERSION     DAOPOA010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22758_length_4224_cov_4.637563"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            436..1200
                     /locus_tag="PKJ17_03405"
     CDS             436..1200
                     /locus_tag="PKJ17_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75047.1"
                     /translation="MSNDPRPEIRTVIVDDERLARKNLRDLLSEHPYVHVIGEAADAA
                     AAREAIVRERPDLVFLDVRMPGGSGFDLLDRLEERPSVIFVTAYDEFAVGAFTNKAFD
                     YLLKPVDPGRLNLSLERFLSSGHDRGQSSAIAQNDRICLNTGKKVVFIELMKIAAVMS
                     EKNYIRIFSVDGECFVMRSPLKEWKERFPADIFMGLDRSLLINRHHVRLLRKRNRNGD
                     IYMEGIEKPFHLGRIALQRFKALMGVTERQECKPLS"
     gene            complement(1304..2506)
                     /locus_tag="PKJ17_03410"
     CDS             complement(1304..2506)
                     /locus_tag="PKJ17_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOD75048.1"
                     /translation="MLKTLNIPDLTPKEEITARIDKLRKGMVEKGIELAVILQNVDIF
                     YFTGTLQRGVLVVSAEQGPQFFVEKSVYRAGMETPLDIIPIKRDKEVKDLLAEKKMLH
                     GRCAMEFDVVPVAICERWKSLLGKDRMEDLSPIIRDGRLVKSEFEVAQFRKSGEIVER
                     VFEKAKDIIKEGIREIDIEAELVAEGRKWGHQGFLRMRGLNQEMMNLYVTHGYSTTIT
                     SGADVPISGIGVTHAIAQGASVNTVKRGIPVIVDYGGGYNGYITDETRAYAVGPLKDL
                     FQKPFEVARDIINETMDTAKEGTDATEVFGRAMDRVKTAGLEEHFMGHGEGQVSFIGH
                     GLGLEINELPVITPRHRMILKEGMVFAFEPKFIFPGEGAVGIEVDFVVRKNGLERLTK
                     TPLDIVYV"
     gene            complement(2481..>4224)
                     /locus_tag="PKJ17_03415"
     CDS             complement(2481..>4224)
                     /locus_tag="PKJ17_03415"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOD75049.1"
                     /translation="RTHISVEGASMLFSKMFIPTVKEDPKEAEVASHRLMLRAGFIRR
                     LASGVYTWLPLGLRSLRKVEQIIREEMNRKGAQEIFMPAVQPKELWVESTRWEKYGKE
                     LLRFVDRNNRELCLGPTHEEVVTDLVRREVKSYKDLPVNLYQIQNKFRDEIRPRFGIM
                     RSREFSMKDAYSFDVDESGAEKSYKEMYEAYVSIFTRCGFRFRVVEADTGQIGGSFSH
                     EFMVLADTGEDIIISCDACGYAANLERAEVGFTDGPVSGKKGLHRRVETPNQRKIDEV
                     SSFLGITPDKLLKTIIYNTDKGTIGVLVRGDREINDTKLKNLLKLEEMELADERTIEE
                     ATGGPLGFSGPIGLSIPLYADMDLTFMEDFVIGGNERDVHVVDVNVGDLTVKGFYDIK
                     VAVAGDRCPRCESGKLVATRGIEVGHIFKLGLKYSKAMNATFLDSEGKDQFMVMGCYG
                     IGVGRTVAAAIEQGNDENGMILPLPIAPFEVDVLPVNTTHEESMKVARQIYEELLANG
                     VDAILDDRDERPGVKFKDCDLIGIPLRITIGERGLKEGIVEIKRRDSKEFEKVPAKEA
                     ARRVMNYVENAKHP"
BASE COUNT          793 a         1280 c         1183 g          968 t
ORIGIN      
        1 gtccccggtg ctctcgggga aaagagcgat ctgcggcatg tactgccgtt ccgttgcgtc
       61 ataggaaaaa tgggaaagat gctcccgatc gagaacgacc tggttctctc cgagaaaccc
      121 cttgacctcg tccaggaact gttcgaaaga catgatggtc aattatatcc gggcattccc
      181 gatggagcaa ggaaattaag gtgcagccgg tcttgtcgaa agagcggtcg gagaagaaag
      241 tccaaatacc gatgtcgcag caggacaggg tgcatcgaca gcctgttggg cacttgtcga
      301 gacacgttag taccaatcat gtttctccgc agccctcgta atgtgtacaa tggcgggtag
      361 ttgatgcttt tgcatccagt tactttatgg cagatcggat tgatccgaca aaccagccag
      421 aaaaagggga acgcgatgtc gaatgatcct agacctgaga tccggacggt gatcgtggac
      481 gacgaacgcc tggcccgcaa gaatctcaga gaccttctga gcgaacaccc ctacgttcat
      541 gtgatagggg aggcggcaga cgcggcggct gcccgggagg ccatcgtccg agagagaccg
      601 gaccttgtct tcctcgatgt aagaatgccc ggcgggtcgg ggtttgacct cctcgacagg
      661 ctggaagaga ggccgtccgt catattcgtg accgcctacg acgagttcgc cgtcggcgcc
      721 tttacgaaca aggccttcga ctatcttctg aagcctgttg acccgggccg tctcaacctt
      781 tcgctcgaaa ggttcctgtc gagcggccat gaccggggac agtcctccgc tatcgcgcag
      841 aacgaccgga tctgcctcaa caccgggaag aaggttgtct tcattgagct catgaagatc
      901 gccgccgtca tgtcggagaa gaactatatc aggatcttca gtgtcgatgg tgaatgcttc
      961 gtgatgcgct cccccctcaa ggagtggaag gagcgttttc ctgcggacat cttcatgggt
     1021 ctcgaccgct ctttactgat caacaggcat cacgtccgcc ttttgagaaa aaggaaccgc
     1081 aacggggata tctacatgga aggcattgag aaacccttcc acctgggccg gatagcgctc
     1141 cagcggttca aggctctcat gggcgtgacg gaaaggcagg agtgcaagcc cctgtcgtga
     1201 atgcttcccc cggtgatgaa acatgcggtc acaggtcaca tgttgcaggt cacaggtcag
     1261 aataacaaaa agatattcag tctggaacct ggaacgtgaa accctagacg tacactatgt
     1321 ccaacggtgt cttcgtcagc ctttccaggc cattctttct gacgacgaag tccacctcta
     1381 tgcctacggc gccttcaccg gggaagatga acttcggctc gaaggcgaag accatgccct
     1441 ctttgagtat catcctgtgc cgcggcgtga tgacgggaag ttcgttgatc tcgaggccga
     1501 ggccgtggcc gatgaagctt acctggcctt ccccatggcc catgaaatgt tcttccagtc
     1561 ccgcggtctt gacccggtcc atggcccttc cgaagacctc cgtcgcgtcg gtcccttcct
     1621 tcgcggtgtc cattgtctcg ttgatgatgt cccgggccac ctcgaagggt ttttgaaaga
     1681 ggtctttcaa gggccccacc gcgtacgccc gcgtctcatc ggtgatgtac ccgttgtacc
     1741 cgccaccgta atcgacgatt accgggatgc cccgtttcac agtgttgacg gaggccccct
     1801 gagcgatggc gtgcgtcacg cctatcccgg atatgggtac atcggccccg gaggtgatcg
     1861 tcgtggagta gccgtgggtg acgtagaggt tcatcatctc ctggttgaga ccgcgcatcc
     1921 tgaggaatcc ctggtgtccc catttcctcc cttccgcaac gagttcggcc tcaatatcga
     1981 tctcccggat cccttccttt atgatgtcct tcgccttttc aaagacgcgc tccacgatct
     2041 cgccggattt tcggaactgg gcgacctcga attcgctctt tacgagcctc ccgtccctga
     2101 tgatcggcga caggtcttcc atcctgtcct tccccaggag cgatttccat cgttcacaga
     2161 tcgcgacggg cacgacgtcg aactccatgg cgcaccgccc gtgaagcatt ttcttctccg
     2221 ccaggaggtc cttgacctct ttgtcccgct tgatggggat gatatcgagg ggtgtctcca
     2281 tcccggcgcg ataaacgctt ttttccacga aaaactgcgg tccctgctcc gccgacacca
     2341 cgaggacccc gcgctggagc gttcccgtga agtagaagat gtccacgttc tgcaggatga
     2401 cggcgagttc gatgcccttc tccaccatcc ccttgcggag cttgtcgatc cgtgccgtta
     2461 tctcttcctt cggtgtgaga tcagggatgt ttagcgtttt caacatagtt catgaccctc
     2521 cgtgccgctt cctttgcagg caccttttcg aattccttgc tgtcccgtcg ttttatctcc
     2581 acgatgcctt ccttgaggcc ccgttcccct atcgtgatcc gaaggggtat cccgatgagg
     2641 tcgcaatcct tgaacttgac acccggtcgc tcgtcgcggt cgtcaaggat ggcgtcgacg
     2701 ccgttcgcca ggagctcctc gtatatctga cgggcgacct tcatgctctc ctcgtgggtg
     2761 gtgttcacgg gcagcacatc cacctcgaag ggggcgatcg gcaggggcag tatcatgccg
     2821 ttctcgtcgt tgccctgttc gatggcggcg gccacggtcc tgccgacgcc gatcccgtag
     2881 catcccatga ccatgaactg gtccttgccc tcgctgtcga ggaaggtggc gttcatcgcc
     2941 ttgctgtact tgaggccgag tttgaagatg tgccccacct cgatgccccg cgtcgcgacg
     3001 agtttgccgc tctcgcaccg gggacagcgg tcgccggcca cggccacctt tatgtcgtag
     3061 aaccctttga ccgtgagatc acccacgttg acgtccacca cgtggacatc acgttcgttg
     3121 ccgcctatca cgaagtcctc catgaacgtg aggtccatgt cggcgtaaag gggaatggaa
     3181 aggccgatgg ggcccgagaa gcctaaaggc ccgcccgttg cctcctcgat ggtcctctcg
     3241 tcggcaagct ccatttcctc cagcttgagg aggttcttca gcttcgtgtc gtttatctcc
     3301 ctgtcacccc ggacgaggac gccgatcgtc cctttatccg tgttgtagat gatggtcttg
     3361 aggagcttgt ccggcgttat cccgaggaag gaggagacct cgtctatctt ccgctggtta
     3421 ggcgtctcca cgcgcctgtg gaggcccttc ttcccggaga cgggcccgtc cgtgaacccc
     3481 acctcggccc tttcgaggtt tgccgcatac ccgcaggcgt cacaggagat gatgatgtcc
     3541 tcgcccgtgt cggcgagcac catgaactcg tgggagaagc tcccccctat ctggccggta
     3601 tcggcctcga cgacgcggaa cctgaacccg caccgcgtga agatgctgac gtaggcttcg
     3661 tacatctcct tgtagctctt ctccgccccg gactcgtcga catcgaagct gtaggcgtcc
     3721 ttcatcgaga actcgcggga gcgcatgatc ccgaagcggg gcctgatctc gtcgcggaac
     3781 ttgttctgta tctggtagag gttgacgggg aggtccttgt aggatttgac ctccctccgc
     3841 acgaggtcgg tgaccacctc ctcgtgggtg ggcccgaggc acagctcgcg gttgttcctg
     3901 tcgacaaagc gcaggagctc cttgccgtac ttttcccacc gcgtgctctc gacccagagc
     3961 tccttcggct ggacggcggg catgaagatc tcctgggcgc ccttccggtt catctcctcg
     4021 cggatgatct gctccacctt tcggagcgac ctgagcccca gaggaagcca ggtatagaca
     4081 cccgatgcca ggcgcctgat gaaccctgcc ctcaacatga ggcgatggct cgcgacctcg
     4141 gcctctttcg ggtcttcctt gacggtgggg atgaacattt tcgagaacag catgcttgct
     4201 ccttcaactg agatatgtgt tcga
//
LOCUS       DAOPOA010000109         4216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_22815_length_4216_cov_4.090123, whole genome shotgun sequence.
ACCESSION   DAOPOA010000109 DAOPOA010000000
VERSION     DAOPOA010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22815_length_4216_cov_4.090123"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKJ17_03420"
     CDS             complement(<1..540)
                     /locus_tag="PKJ17_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD75050.1"
                     /translation="MEKQRRLRFTVLCENVVANLRGIGEHGFAVFVETETGTYLFDTG
                     VGIGILRNSLTFSRDLRSVQKILLSHGHYDHTGGLKDVLSVTGPIEVHGHPAIFDEKY
                     ALEHAGEDMTVRFIGMPERRESLEASGADFHLGSGFRDIAKGVYLTGEIPRLSSFETG
                     DKRLAVKKGETYDVDTVPDD"
     gene            850..1602
                     /locus_tag="PKJ17_03425"
     CDS             850..1602
                     /locus_tag="PKJ17_03425"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HOD75051.1"
                     /translation="MKLEGKNAIVTGGGRGVGRAISLAFAQEGANVVINYAGNQKAAD
                     EVVELIKGMGRKAVAVKGDVGQEADAQKIVETCGEAFGSVHIIINNAGISKPSMMHKM
                     SVETWDEVVNVQMRGPWLMAKAASKYFMQQNYGRIVNVTSVAGVVGTIGQINYSAAKG
                     GVISMTKSMARELAKFNVTANVISLGIVTTEMTHTISTDEKLKEIYTKRILLGRYADP
                     EDVAPAFVFFASDDARYITGQLLCVDGGYGMT"
     gene            1654..2763
                     /gene="had"
                     /locus_tag="PKJ17_03430"
     CDS             1654..2763
                     /gene="had"
                     /locus_tag="PKJ17_03430"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: protein motif:HMM:TIGR03201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HOD75052.1"
                     /translation="MADVPKTIKQWQMVQPTVFNRETKETTPGKLEKAEIPVPELKTG
                     EVLVEIAGCGVCHTDLGYFFDGVPTVSKPPLALGHEIAGTVIAGDPAWIGKEVIIPAV
                     MPCRQCILCKTGRGNRCLAQKMPGNSIGVYGGFASHIVVPTIDLCLVQDKKGYKLEQL
                     AVVADAVTTPYQACKRADLQPGDNVIIIGATGGVGVYMAQTAKALGAKTVIGIARNQE
                     KLQRALGYGCDYVISTLDKTNKDVVGEWRNICKANGLANTGWKIFEVTGSKAGQDLAL
                     DLLSFVGKLILVGFGMAKSEYMFSKLMAFDAEVIGTWGCLPEYYPIVLDMVLSKKIQI
                     DPFVEVRPMSTIAATFEEIHKAGSPAKRVVLTPDF"
     gene            2795..3940
                     /gene="oah"
                     /locus_tag="PKJ17_03435"
     CDS             2795..3940
                     /gene="oah"
                     /locus_tag="PKJ17_03435"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HOD75053.1"
                     /translation="MGLEWMPREHEKKDHFRFTDQHWGTEAPCVVYERKPLKDNKGNV
                     VPGLYVAWIRLNNPSQFNSYTTEMVKGVIAGFENSSVDREVVGVVFTGTGPFAFCTGG
                     NTKEYSEYYSRRPEEYGAYMELFNNMVDSILMCKKPVISRVNGMRVAGGQEIGTACDI
                     TLTSDLAIFGQAGPRHGSAPVGGASDFLPSYMSIEDAMWSCISCEMWSAYKMWWKGLL
                     TKAFPVLKDDKGNWVRNPQVITDKFIENGEIVYGENKAGQEFKDARAWVGEKQKAGDV
                     DFSLMDAEIDRIMWTYANLFPGCLMKSIDGIRQKKKFFWDTMKNEHRHWLAANMGIEA
                     FLGFGAFNTKKITGMDTIDFIKYRKGVADGVLMDDTFMAEVLGKPQK"
     gene            4005..>4216
                     /locus_tag="PKJ17_03440"
     CDS             4005..>4216
                     /locus_tag="PKJ17_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOD75054.1"
                     /translation="MAFTHITFQKKNKVATITLNEPPSNWLTILMMREIVEAIMDVKK
                     DPTIQLLVFDHAGEKAFCDGVDVADHT"
BASE COUNT         1045 a         1189 c         1177 g          805 t
ORIGIN      
        1 gtcatcgggc acggtgtcga catcataagt ctcgcccttc ttgacggcaa gccgtttgtc
       61 acctgtttcg aaggatgaaa gccgcggtat ctcgccggtg aggtacaccc ccttcgcgat
      121 gtcgcggaaa ccggagccca ggtggaaatc cgcgccgctc gcctccagcg actctctcct
      181 ctcgggcatg ccgatgaaac ggaccgtcat gtcctctccc gcgtgctcca gggcatattt
      241 ctcatcgaat atggcggggt ggccgtggac ctctattggt cccgtcacgc tgagaacatc
      301 cttcagcccc ccggtatgat cgtagtggcc gtggcttaac aggatctttt gaaccgaccg
      361 caggtccctc gaaaaggtga gggagtttct gaggatgcca ataccgacac cggtgtcgaa
      421 aagatacgtc ccggtctccg tttccacaaa aacggcaaaa ccgtgctcac cgatgccgcg
      481 gagattggca accacattct cgcaaagaac ggtgaaacga aggcgccttt gtttttccat
      541 ggcaaacaac ctttcaaaga aatcgggggt tttctctgtc ttttcaatga ttaaccatat
      601 cccggctttt ttcaagcgaa aaaggcccac cgtcacccct tccggggcca acaaaacacc
      661 atttccgaat tcccgttgac agggctttgt gaaaagccgg aacaccctta atatcaagac
      721 tttttatctt gacatagctc ccttctggaa gataatatta agtttataaa aattttcaca
      781 aatcaagctg cagcaccttt agaagttgga aaacagaagt tagaaaacga cgaaaaggag
      841 agggccgtta tgaagttaga gggtaagaac gcaattgtaa cgggtggcgg cagaggcgtg
      901 ggaagagcca tcagtctggc ctttgctcag gaaggcgcga atgtggtcat caactatgcc
      961 ggaaaccaga aggcagccga cgaggttgtc gagttgatca aggggatggg caggaaggca
     1021 gttgccgtga agggtgacgt cggtcaggag gcggatgccc agaagatcgt ggagacctgc
     1081 ggcgaggctt tcggatccgt ccacatcatc atcaataacg cgggtatctc caaaccttcg
     1141 atgatgcaca agatgagcgt ggagacctgg gacgaggtcg tgaacgtcca gatgaggggt
     1201 ccctggctca tggcgaaggc cgcgtcgaag tatttcatgc agcagaacta cggcaggatc
     1261 gtcaacgtga catccgttgc cggcgtggtc ggcaccatcg gtcagatcaa ttacagcgcc
     1321 gccaagggcg gggtgatctc catgaccaaa tcgatggcgc gggagctcgc gaaatttaat
     1381 gtcacagcca acgtcatatc actgggcata gtcaccaccg agatgaccca taccatatcg
     1441 acggacgaga agctcaaaga aatctacacg aaacgcatcc tcctcggacg atacgcggac
     1501 ccggaagatg tcgcaccggc cttcgtattt tttgcttccg atgacgcccg ctatattacg
     1561 ggtcaactgc tgtgcgtaga cggcggttac ggaatgacgt aagaactgag ggaggtgatc
     1621 tacgttcact caaatatata aggaggtgtt gtaatggctg atgtaccaaa aacaataaag
     1681 cagtggcaga tggtgcagcc caccgtcttc aacagagaga cgaaagagac gacccccggc
     1741 aagctcgaaa aggcagagat cccggttccc gaactgaaga caggcgaggt gctggtcgag
     1801 atcgccggct gcggcgtatg ccacacggac ctcggatact tctttgacgg tgttcccacc
     1861 gtgagcaaac cgcctctggc gctgggtcac gagatagccg gcaccgttat cgccggtgat
     1921 cccgcatgga tcggcaaaga ggtcatcatt cccgcagtta tgccctgcag gcagtgcata
     1981 ctctgcaaga ccggcagggg caacaggtgc ctggcccaga agatgcccgg caactcgata
     2041 ggcgtgtacg gcggcttcgc gagccacatc gtcgttccca cgatcgacct gtgccttgtc
     2101 caggacaaga aaggttataa actggaacag ctcgccgttg tggcggacgc agtgaccacc
     2161 ccgtaccagg cgtgcaagag ggccgacctt cagcccggtg acaatgtcat catcatcggc
     2221 gcgacgggcg gcgtgggcgt gtacatggcc cagaccgcga aggcgctcgg ggccaagacc
     2281 gtcatcggca tcgccaggaa ccaggagaaa ctccagaggg ccctgggcta cggctgcgac
     2341 tacgttatca gcaccctcga caagacgaac aaggatgtcg tcggcgagtg gaggaacatc
     2401 tgcaaagcca acggcctcgc caacacaggc tggaagatct ttgaggtcac cggctccaag
     2461 gccggccagg atctcgccct cgatcttctc tccttcgtgg gcaaactcat cctcgtcggc
     2521 ttcggcatgg cgaagagcga gtacatgttc tccaagctca tggccttcga cgccgaagtt
     2581 atcggcacct ggggctgtct gcccgagtac tacccgatcg tcctcgacat ggtcctcagc
     2641 aagaagatac agatcgaccc cttcgtcgaa gtgcggccga tgagcaccat cgcggcgacc
     2701 ttcgaggaga tccacaaggc aggttcacct gcgaaacgcg ttgtcctgac acctgatttt
     2761 taaggtttca aacaaaaact tttaggagga aagtatggga ttagaatgga tgccaagaga
     2821 acatgagaag aaagaccatt tcaggttcac cgatcagcac tggggaacgg aagccccctg
     2881 cgtagtgtac gaaagaaaac ccttgaagga caacaagggc aacgtcgtcc ccggcctgta
     2941 cgtagcatgg atcaggctca acaacccctc acagttcaac tcctacacga ccgagatggt
     3001 caagggcgtc atcgccggtt tcgagaactc atccgttgac cgcgaggtcg tcggcgttgt
     3061 cttcaccggc acgggcccct tcgcattctg caccggcggc aacacgaaag agtactcaga
     3121 gtactacagc agacggccgg aagaatacgg cgcgtacatg gaactcttca acaacatggt
     3181 cgactccatc ctcatgtgca agaagccggt catctcccgc gtgaacggca tgagggttgc
     3241 cggcggccag gagatcggca ccgcctgcga catcaccctt acctctgacc tcgccatttt
     3301 cggtcaggca ggccccaggc acggttccgc ccccgtcggc ggcgcctccg acttcctccc
     3361 ctcctacatg agcatcgaag atgcgatgtg gagctgcatc agctgcgaga tgtggtcggc
     3421 atacaagatg tggtggaagg gcctcctcac caaggcattc cccgtcctga aagacgacaa
     3481 gggcaactgg gtacggaacc cgcaggtcat caccgacaag ttcatcgaga acggcgagat
     3541 cgtctacggc gagaacaaag ccggtcagga gttcaaggat gcgcgggcgt gggtcggcga
     3601 aaaacagaaa gccggcgacg tcgacttctc cctcatggat gcggagatcg acaggatcat
     3661 gtggacctac gcgaacctct tcccgggctg cctcatgaaa tccatcgacg gcatccgcca
     3721 gaagaagaaa ttcttctggg ataccatgaa gaacgagcac cgtcactggc tcgcggccaa
     3781 catgggcatc gaggccttcc tcgggttcgg cgcgttcaac accaagaaga tcaccggaat
     3841 ggacaccatc gatttcatca agtaccgcaa gggcgtcgcg gacggcgttc tcatggacga
     3901 taccttcatg gcggaagttc tcggcaaacc tcagaagtaa ttacgaggca aactctaggg
     3961 gggagcacat cccccctatt ttttttgaaa gaaggagaac catcatggct ttcacgcaca
     4021 taacatttca aaagaagaac aaggtcgcaa cgataaccct caacgagccc ccctcgaact
     4081 ggctgaccat cctcatgatg cgggagatcg tcgaggcgat catggacgtc aagaaagacc
     4141 cgacgatcca gcttctcgtc ttcgatcacg ccggagaaaa ggcgttctgc gacggcgtcg
     4201 acgtggccga ccacac
//
LOCUS       DAOPOA010000110         4204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_22902_length_4204_cov_4.015184, whole genome shotgun sequence.
ACCESSION   DAOPOA010000110 DAOPOA010000000
VERSION     DAOPOA010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22902_length_4204_cov_4.015184"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..2025)
                     /locus_tag="PKJ17_03445"
     CDS             complement(112..2025)
                     /locus_tag="PKJ17_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD75055.1"
                     /translation="MYRKLIGTRDIGFVVLNDKNVVVDANTKYARLAGRRDIDHVLGK
                     SILDWTAQHDRDRLSRELAGCLKKGFARSVEIDHTDGEGGITPVEMNVTRIGAGSGKD
                     LVVFCRDVPKQTRTAGKLRYRAGGPAGLTEVAPFALRSTLFSVLENLPLGIILADRSG
                     VGTYVNPQFTGITGYTLDDVPTEKDWAVRAYPDLELRKTVVNAWKRNRSPGSIYESAE
                     RPIACKNGREKVISLRATRLEKLTVITLSDAAKQGQTGSALRHNGDRYRSIYENAIEG
                     IFRAAPDGRFLSANPSHARMLGYDSSEDMVRDIASIGRELFLDPENHRQFRETLQTRG
                     VVEKFETRLRRRDGGTIWVSLNARAARDSAGEIAYYEGTAEDITDRKAMEEEKRQLEA
                     RLRQSEKMEAIGKLAGGIAHDFNNILEVLLGHCGILKMQAPKDGPVQERVRQIANTIE
                     KASSFTRSLLAFSRKQDVVLKPVAINAMVRKSVRILEGLLPKTIALDMALHGGDATVL
                     IDVSKIDQVLLNLVANARDAMPRGGKITIRSRTVSLDEDFRRLYGYGTPGRYVLISVT
                     DTGQGIDDGVMHRIFEPFYTRKKPGKGTGLGLAIVYGIVKQHNGYINASSKPGKGTTF
                     NVYLPVATIRKPA"
     gene            complement(2107..2739)
                     /locus_tag="PKJ17_03450"
     CDS             complement(2107..2739)
                     /locus_tag="PKJ17_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD75056.1"
                     /translation="MSEDHYRSLVDLSPDIIYRLDGEGRILFISNAVRELGYDPEELT
                     GRLFEDIVHPDDRGKGPVHFVERRVGSRSMQRLEMRLIKKSGAVQNYSLAQVTVELTA
                     RGKWDVPDSDISNPRKNLLYSQGVARDVTERKRMEVERDKLILELKEALLRVRTLSGL
                     LPICSSCKKIRDDDGRWQPLENYVREHSEAEFTHTICPECARKTYPNYFK"
     gene            3236..4114
                     /locus_tag="PKJ17_03455"
     CDS             3236..4114
                     /locus_tag="PKJ17_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD75057.1"
                     /translation="MHDITENGRSKELLLKVRRFRAIFDHTFQFMGLLTPGGNVLEVN
                     GAALAFAGIREEEVVGRRFWLTPWWSHSRKLMNILRDAVKEAAGGRLMRFEATHLSSE
                     GRPHYFDFSLKPVPGEDGKVAFLIAEGRDVTDRKAVEEALRKREEELEFKTRNLEEAN
                     IALKALARHIEENRQEFERNIVLNVRTLVFPYIEKMQRSNLNIRQKMFMEVIEKNLGN
                     VISPFLKNISHFGLTPTEMHIAGLIRDGRKTKEIAESLHISRRAVEVHRYNIRKKLKM
                     NRSRQNLCAFLRSLGS"
BASE COUNT          793 a         1230 c         1242 g          939 t
ORIGIN      
        1 aacccctcag gaaaaggcag ctcgccgaag cggtgaggaa ggtcctcgat accagggaat
       61 gatccacggg gactcctccg gcaccgcccc gaacatgaac cgctcttccg gtcatgccgg
      121 ctttcggatc gttgccacgg gcaaatagac gttaaaagtc gttccttttc caggtttgct
      181 ggaggcgttg atgtacccgt tgtgctgttt cactattccg tagactatgg caaggccgag
      241 gcccgttccc tttcccggtt tcttcctggt atagaagggt tcgaatatcc tgtgcatcac
      301 tccgtcatca atgccttgtc ccgtatcggt aacggagatg aggacgtacc tgccgggggt
      361 gccgtagccg taaagcctcc ggaaatcttc gtcgaggctg accgttctcg accgtatggt
      421 gatcttgccg ccccggggca tggcgtcgcg ggcattggcg acgaggttca gcaatacctg
      481 gtcgatcttt gatacatcaa tgaggactgt cgcgtccccg ccgtggaggg ccatatcgag
      541 ggcgatggtc ttcggcagca gcccctcaag gatgcgcacg ctctttcgaa ccatcgcgtt
      601 gatcgccacg ggtttgagca cgacgtcctg tttcctgctg aacgcgagaa ggcttctcgt
      661 gaagctcgat gccttctcta tcgtgttggc gatctgcctc acgcgttcct gcactggacc
      721 gtccttcggc gcctgcatct tcagaatgcc gcagtggcca aggaggacct cgaggatgtt
      781 gttgaagtcg tgggcgatgc cccctgccag cttgccgatg gcctccatct tttccgattg
      841 gcgaagccgc gcttcgagct gtctcttctc ctcctccatc gccttccggt ccgtgatgtc
      901 ttcggcggtc ccttcgtagt aggcgatctc gcccgcgctg tcccgcgccg cgcgggcgtt
      961 gagggagacc catatggtgc cgccgtccct gcgacgcagc cgggtctcga acttttcgac
     1021 gacgccccgc gtttgcaggg tctcacggaa ttgccggtgg ttctcggggt ccaggaagag
     1081 ctctcgacct atgctggcaa tgtcccggac catgtcctcc gacgaatcgt atccgagcat
     1141 tcttgcatgc gacgggttcg cgcttaagaa acgcccatcc ggcgcagccc ggaagatgcc
     1201 ctcgatggcg ttctcgtata tgctgcggta cctgtctcca ttgtgcctga gggccgaccc
     1261 tgtctgtccc tgttttgctg cgtcgctcaa ggttatgacg gtgagtttct cgagacgggt
     1321 cgcccgcaac gatatgacct tttcccgtcc attcttgcag gcgatcgggc gctccgctga
     1381 ctcatagatg cttcccggcg aacgatttct cttccaggcg ttgaccaccg ttttcctgag
     1441 ttccaggtcg gggtacgccc ggacagccca atccttctcg gtgggcacgt catcgagggt
     1501 gtagccggtg atgccggtga actgcgggtt gacgtacgtg cccaccccgg aacgatcggc
     1561 caggatgatg ccgagaggaa ggttctcaag gacgctgaag agagtgctcc tcaacgcgaa
     1621 gggggcaacc tccgtgaggc ccgccggccc gccggcccgg tatcggagct ttccggccgt
     1681 acgtgtctgc ttggggacat cgcgacagaa cacgacaaga tccttcccgg agccggcacc
     1741 gatgcgcgtg acgttcatct cgacgggcgt gatgccgccc tccccgtcag tgtggtcgat
     1801 ctcgacgctt ctggcgaaac ctttcttcag gcatccggcg agctcccggg aaagcctgtc
     1861 ccggtcgtgc tgcgcggtcc agtcaaggat gctctttcca agcacatgat cgatgtcgcg
     1921 gcggcctgca aggcgggcgt atttcgtatt cgcgtccaca acgacgtttt tgtcgttgag
     1981 gaccacaaag cctatgtccc gtgttccgat cagcttccga tacagctcct cgctctgtat
     2041 aatggtccct ccctgcctct tgttcagact ccttcaacgt gaaacgcgat ggagaccgcg
     2101 accctctcac ttgaagtagt ttggatacgt ttttcttgca cattccggac aaatggtgtg
     2161 ggtgaactcg gcttcggaat gttcccgcac atagttttcc agcggctgcc agcgtccgtc
     2221 gtcgtctcgg atcttcttgc aggagctgca tatgggcaag aggccgctca aggtccgcac
     2281 cctgagcagg gcctctttga gttccaggat gagtttgtcc cgttccactt ccatgcgctt
     2341 gcgttccgtg acgtcgcggg cgacgccctg ggagtaaaga agattttttc gcggattcga
     2401 tatgtcggaa tcggggacgt cccatttccc cctggcggtg agctccacgg tgacctgtgc
     2461 aagtgaatag ttctggaccg ccccgctctt cttgatcagg cgcatctcca gcctttgcat
     2521 ggacctcgag ccgacgcgtc tttcgacgaa atggaccggc cccttgccgc ggtcgtcggg
     2581 gtggactatg tcctcgaaga gcctccccgt gagctcctcc gggtcgtagc cgagttccct
     2641 gacggcgttg gagatgaaga gaatccgtcc ttcgccgtcg aggcggtaga tgatgtcggg
     2701 gcttaagtcc acgagggaac ggtagtgatc ttcgctcaag agacaggtcc tcccgaacat
     2761 atgcgatagt ctgtttccat tgcgatgggt ttcatctgtt ctgtttgcgg gcgaacctgg
     2821 cgtgccaggg tgttctcgcc cccctggcat cactttgggt cgaacgggct ggacggcatg
     2881 acaaaaccgt tcttctcgtc ggtggtgtgg gactgcctgc cttcaactcc cccgatcaaa
     2941 aggaaccccc tgtccttccg acacccggcg ccttccgcgc gcccttaagg ttccctgccg
     3001 ttgtgattcg taattgtatg caaaaagaca gaagaatgct atagcaaata acttgctatg
     3061 gaatacgtac gcgttgctag tagtatggcg ccccggaaag ggggtcaggg gagtccgcgc
     3121 gctatcgccg ggcacgcccc ttttaagtaa aaccccataa tgttcgacta ttattctaat
     3181 cgggtataag gcgcatcatg atcacgtacg gaaacggagg gacgtacccc gggccatgca
     3241 tgatatcacc gaaaacggaa ggtcgaagga gctccttctc aaggtgcgaa gatttcgcgc
     3301 gatatttgac cacacgttcc agtttatggg cctgttgacg cccggcggca acgtgctgga
     3361 ggtgaacggg gcagcgctcg ccttcgccgg gatccgggaa gaggaggttg ttgggcgcag
     3421 gttctggctg acgccgtggt ggtcccattc cagaaagctg atgaacatac tccgggatgc
     3481 cgtgaaagag gcggcgggcg gaaggcttat gcgcttcgag gcgactcatc tctcgtcgga
     3541 aggccgcccc cactattttg atttttcgct gaaacccgtg ccgggtgagg atggaaaggt
     3601 tgcctttctc atcgccgaag gccgcgacgt aacggaccgc aaggcggtcg aggaagcact
     3661 gaggaagaga gaagaggagc ttgagttcaa gacgcggaac cttgaagagg cgaacatagc
     3721 cctgaaggcg ctcgcgaggc atatcgaaga gaacaggcag gagtttgaga ggaacatcgt
     3781 cctcaacgtc aggacgctcg ttttcccgta catcgagaag atgcagagat cgaacctcaa
     3841 catcaggcag aagatgttca tggaggtcat cgaaaagaac ctcggcaacg tgatctcgcc
     3901 cttcctgaag aacataagcc acttcggcct cacacccacg gagatgcata tcgcgggcct
     3961 gatcagggat ggaaggaaga cgaaggagat cgctgaatcg ctccatatat cccgccgggc
     4021 cgtcgaggtc caccgctaca atatcaggaa gaaactgaag atgaacagga gcaggcagaa
     4081 cctgtgcgct ttcctccgct ccctgggcag ttgatccgaa cggagacgcc gcatcggcgg
     4141 atcgctccgc tttcaccggt gacagcgggc tgtttccggt ttgccgcggc aggtcatcct
     4201 cccg
//
LOCUS       DAOPOA010000111         4190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_22989_length_4190_cov_3.902781, whole genome shotgun sequence.
ACCESSION   DAOPOA010000111 DAOPOA010000000
VERSION     DAOPOA010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22989_length_4190_cov_3.902781"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PKJ17_03460"
     CDS             complement(<1..459)
                     /locus_tag="PKJ17_03460"
                     /inference="COORDINATES: protein motif:HMM:NF017501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOD75058.1"
                     /translation="MDDKLIVAGREFGSRFFLGTGKFGDKDAMRKAIASSGSQLVTVA
                     LRRIDLDETDENILSFIPEGVTIMVNTSGARNAEEAVRIAHIAREAGYGDWIKIEVIN
                     DSRYLLPDNQETIKATKVLSGEGFVVLPYMHPDLYVAKALVDAGAAAVMPL"
     gene            complement(461..661)
                     /gene="thiS"
                     /locus_tag="PKJ17_03465"
     CDS             complement(461..661)
                     /gene="thiS"
                     /locus_tag="PKJ17_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOD75059.1"
                     /translation="MKITVNGRTMEIAEGMDLHRLVHSGKADPGRVILVLNDGVVKSD
                     RWSLTVLSEGDRVELVSFVGGG"
     gene            complement(658..1251)
                     /gene="thiE"
                     /locus_tag="PKJ17_03470"
     CDS             complement(658..1251)
                     /gene="thiE"
                     /locus_tag="PKJ17_03470"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOD75060.1"
                     /translation="MTRRKPFSNDLYVISSSVGELKRAVDDGAAIVQLRDKTGDEATV
                     LEKARELLEYRKIRPFVFILNDDPVLAVKVGADGVHVGQDMSTLEARAIVGEEMIIGK
                     TTHNLEQGRQAIKDGADYISTGPVYATPTKPGRAPVGLAYVREAAEHLDIPAVAIGGI
                     DLSNIDDVLAAGAKTIGVVRASSDAVELLKRIRKAAK"
     gene            1441..2277
                     /gene="nifU"
                     /locus_tag="PKJ17_03475"
     CDS             1441..2277
                     /gene="nifU"
                     /locus_tag="PKJ17_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HOD75061.1"
                     /translation="MWEYTDKVREHFLNPKNAGEIEDPDGVAEVGSIACGDALKLSFK
                     LDENKRIADAKFKTFGCASAIASASALTEIIKGKTIEEAMKITNQDIADYLGGLPQEK
                     MHCSVLGQEALEKAIDNYRGVSHKKPEEEIVCACFGVTDGEIERAIRQNHLTSVEEVT
                     YYTKAGGGCGNCHDKIQAILDRVLAAKRSEKEPEERPRLSNIQKIRMIEETIEREIRP
                     SLQHDGGDIDLIDVIGNRVLVATRGACATCQASQITLKSFVEWKLKEFVSPDLVVEEV
                     AK"
     gene            2274..3455
                     /gene="nifS"
                     /locus_tag="PKJ17_03480"
     CDS             2274..3455
                     /gene="nifS"
                     /locus_tag="PKJ17_03480"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOD75062.1"
                     /translation="MRTVYLDNNATTMVSREVLDEMLPFFHDRYGNPSSMHSFGGRVA
                     RELSDARHKVASLLGADPEEIIFTSCGTESDNTAIMSSLLVKPGKTHIVTSRVEHPAI
                     KVLCEHLAQKGYRVTMVGVDSDGRLDMDEYEASLTPDTAIASIMWANNETGVIFPVEE
                     AARLARDRGIVFHTDAVQIVGKIPIDMKGNAVDMLSLSGHKLHAPKGIGVLYVRKGTK
                     FSPFLIGGHQEKGRRGGTENVAGIVGLGKACELAAASMEKENTYVRALRDRLENSLLA
                     AIPQSRVNGVRDERLPNTTNISFEFVEGESILLLMDQHGICASSGSACTSGSLQPSHV
                     LRAMGVPYTMAHGSIRFSLSVYNTEEDIDLVIRELPPIIDKLRVLSPYWATERGACTT
                     V"
     gene            3468..>4190
                     /locus_tag="PKJ17_03485"
     CDS             3468..>4190
                     /locus_tag="PKJ17_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75063.1"
                     /translation="MQERENSRDLCREEIDATQKHRSRLPLLIDEVVAQYDKEGRLHH
                     IGPEPIPSRQAIINIIRKTIPILFPGFFAEKRLDEINARYYLGEQITELFDAISTQIQ
                     HAIRHDCIRFDRPCVHCEDLSQEITLRFIGSIPRLQETLSKDVRAAFQGDPASRHFDE
                     IIFCYPGLFAVAVYRVAHWLHVNEVPLIPRIMTEYAHSLTGIDIHPGVVIGESFFMDH
                     GTGVVIGETTTIGDRVRLYQGVT"
BASE COUNT          914 a         1276 c         1211 g          789 t
ORIGIN      
        1 gagaggcatg acggctgccg cccccgcgtc gacgagcgcc ttcgccacgt agaggtcggg
       61 atgcatgtag ggcagcacga cgaagccttc ccccgagagg accttcgttg cctttatcgt
      121 ctcctggttg tcgggcagca ggtaccggct gtcgtttatg acctcgatct tgatccagtc
      181 cccgtatccc gcctcccgcg ctatgtgtgc gatgcgcacg gcctcctccg cgttgcgcgc
      241 gcccgacgtg ttgaccatga tcgtcacgcc ctcgggaatg aaggagagga tgttctcatc
      301 cgtttcgtcg aggtcgatcc ggcgaagcgc gaccgttaca agctgagagc cggagctcgc
      361 gatcgccttg cgcatggcgt ccttgtcgcc gaactttcca gtgccgagga agaagcggct
      421 cccgaactcc cttccggcca cgatcagttt gtcatccata tcaccctcct cccacgaagg
      481 acaccagttc aacccggtca ccctcagaaa ggacggtcag ggaccaccgg tcgctcttca
      541 cgacgccatc attgaggaca aggatcaccc tgccggggtc cgccttgccg gaatggacaa
      601 gcctgtggag gtccatacct tccgcgatct ccatcgtcct gccgttgact gttatcttca
      661 tttcgctgcc ttccgtattc tcttcagcaa ctccaccgcg tccgatgagg ccctgacaac
      721 acctatcgtc ttcgcccccg cggccagcac gtcatcgatg ttggacaggt cgatgccgcc
      781 gatggcgacg gcagggatat cgagatgctc cgccgcttct ctcacgtagg caagccccac
      841 cggggccctg ccgggtttcg tcggagtggc gtagacgggc cccgtggata tgtaatcggc
      901 gccgtccttt attgcctgcc gcccctgctc caggttgtgc gtcgtcttgc cgatgatcat
      961 ctcctcaccg acgatggcgc gggcctcgag cgtagacatg tcctggccca cgtggacccc
     1021 gtccgccccg accttcacgg ccaggaccgg atcgtcgttg agaatgaaca cgaagggccg
     1081 gatcttcctg tattccagaa gctcccgggc cttttcaagg accgtcgcct cgtcgcccgt
     1141 cttgtcccgg agctgaacga tggcggcgcc gtcatccacc gccctcttca gttcgccgac
     1201 ggacgaggaa atgacataaa ggtcgttgct gaatggtttt ctcctcgtca tcgctgctat
     1261 taaattacat caaaatcgat gacaagtcca aggttctgtg caggtccggc cggaatgcat
     1321 gatgacgcct gaacacgacc ctttgcaagg aagagcaaaa gaggtagaat agaacatgag
     1381 cgtgccccct gagatgagag cggcccgcct taacaaagac ccggaggaaa ggagcgagcc
     1441 atgtgggaat atacggacaa ggtcagggag cattttctca atccgaagaa cgcgggtgag
     1501 atcgaggatc cggatggtgt cgccgaagtg ggttccatag cgtgcggcga cgctcttaaa
     1561 ctcagtttca aactcgatga gaacaaacgg atcgccgacg cgaagttcaa gaccttcggc
     1621 tgcgcgagcg ccatcgcctc cgcgtcggct ctgacggaga taatcaaggg aaagaccatc
     1681 gaggaagcga tgaagataac caaccaggac atcgccgact acctgggcgg tctaccccag
     1741 gaaaagatgc actgctccgt gctcggtcag gaggccctcg aaaaagcgat cgataattac
     1801 cgcggcgtat cgcacaagaa acccgaagaa gagatcgtct gcgcctgttt cggtgtcacc
     1861 gacggcgaga tcgagcgggc gatcaggcaa aaccacctca cgtccgtcga ggaggtgacg
     1921 tactacacga aggcaggggg cgggtgcggg aactgtcatg acaagatcca ggccatactc
     1981 gaccgcgttc tcgcggccaa gagatccgaa aaagaaccgg aggaaagacc aaggctttcc
     2041 aacatccaga agatccgcat gatcgaagaa acgatcgaac gcgagatccg cccttcgctg
     2101 cagcatgacg gcggtgatat agacctgatc gacgtcatcg ggaacagggt cctcgtggca
     2161 acgagggggg cctgcgcaac gtgccaggca tctcagataa ctctgaagag tttcgtcgag
     2221 tggaaactga aagagttcgt ctcgcccgac ctcgttgttg aggaggtggc aaaatgagga
     2281 cggtctacct tgacaacaac gcgacaacga tggtttcccg ggaggtcctt gacgaaatgc
     2341 tccctttttt ccatgatcgg tacgggaacc cctcgagcat gcactccttc ggcggccggg
     2401 tggcccgcga actttccgat gcgcgccaca aggttgcctc gctcctcggg gccgaccccg
     2461 aggagatcat cttcacgagc tgcggcactg aaagcgataa cacagcgatc atgtcctccc
     2521 ttctcgtgaa accggggaag acccacatcg tcacaagcag ggtcgaacac ccggcgatca
     2581 aggtcctgtg cgagcacctt gcccagaaag ggtaccgcgt gacgatggtg ggtgtggaca
     2641 gcgatggcag gctcgatatg gacgaatatg aggcaagtct gaccccggac acggcgattg
     2701 cgagcatcat gtgggccaat aacgaaacgg gcgtcatttt tccggtggaa gaggcggcgc
     2761 gtctggcccg cgaccggggg atcgtctttc ataccgacgc ggtccagatc gtgggcaaga
     2821 tacccatcga catgaagggc aacgcggtcg atatgctctc cctttcgggt cataagctgc
     2881 acgcgccaaa aggcatcggc gtcctttacg tacgaaaagg gacaaagttc tctccctttc
     2941 tcatcggcgg ccaccaggaa aaagggcgcc ggggcggtac ggagaacgtc gccggcatcg
     3001 tcggcctcgg gaaggcctgc gagctcgcgg ccgccagcat ggagaaggag aacacgtacg
     3061 tgagggccct tcgcgacagg ctggagaaca gccttcttgc cgcgatcccg caaagccggg
     3121 tgaacggcgt ccgcgatgag cgtcttccca acacgacgaa catcagcttc gagttcgtcg
     3181 agggtgaaag catcctcctg ctcatggacc agcacggcat ctgcgcttcc tcgggctcgg
     3241 cctgcacatc cggctcgctg cagccttccc atgtcctgcg ggccatgggt gttccctaca
     3301 caatggccca cggatcgata cggttcagcc tcagcgtcta caacaccgaa gaggacatcg
     3361 accttgtcat tagagaactg ccgccgatca tcgataaact ccgggtcctt tcaccttact
     3421 gggcgacgga acgaggcgcc tgtacgacgg tctgaggaga gagagatatg caagaaaggg
     3481 agaattcacg agacctatgc cgggaagaga tagacgcgac ccagaagcat cgctcgcggc
     3541 tgccgcttct catcgacgag gtggtggcac agtacgacaa ggaaggccgt cttcatcaca
     3601 tcggtcccga accgatacct tcacgacagg ctatcatcaa catcatacgc aagaccatcc
     3661 ccattctctt tcccggtttc ttcgcggaaa aacggctgga cgagatcaac gcccggtatt
     3721 acctggggga acagataacg gaactcttcg acgcgatctc gacacagata cagcatgcca
     3781 tacgccatga ctgcatcagg ttcgaccggc cctgtgtcca ctgcgaggat ctcagtcagg
     3841 agataaccct gaggttcatc gggagcattc cccggctgca ggagacgctc tcgaaggatg
     3901 ttcgcgccgc ctttcaaggc gacccggcct cgcggcattt cgacgagatc atattctgct
     3961 atcccggcct cttcgcggtt gccgtctacc gcgtcgcgca ctggctgcac gtcaacgaag
     4021 tgcccctcat cccgaggatc atgacggaat atgcccacag tctgacgggc atcgacatac
     4081 accccggggt tgtcatcggt gagagctttt tcatggacca cggtaccggc gtcgtcatcg
     4141 gggaaacgac caccatcggc gacagggtgc gcctctacca gggcgtaacc
//
LOCUS       DAOPOA010000112         4168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_23150_length_4168_cov_4.611719, whole genome shotgun sequence.
ACCESSION   DAOPOA010000112 DAOPOA010000000
VERSION     DAOPOA010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23150_length_4168_cov_4.611719"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKJ17_03490"
     CDS             <1..224
                     /locus_tag="PKJ17_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75064.1"
                     /translation="TKATVIRIARTIINAFFMLFSPPPLLLVTLSCMGTVYAVVVPLS
                     QQIDIKGKRFGVSFGEAAIIGNIRLRNAL"
     gene            complement(240..1712)
                     /locus_tag="PKJ17_03495"
     CDS             complement(240..1712)
                     /locus_tag="PKJ17_03495"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD75065.1"
                     /translation="MKDDTKGTILVVEDDRNQRGIIKTILSKEGFYVEAAECGKKAVD
                     ILKNGSFDVVLTDLKLPDIDGTEVLREVRALGRTCHVIIMTAFGSIPSAVEATKLGAF
                     YYLEKPLEKDQLLLVITNAMNQVKLLKDNIMLKDQLRDRFHIDNIVGAHGAMEELYKI
                     VRRVAPTNSTVLVHGESGTGKELFAKSIHYNSPRKGKPFFAINCAAIPESLLESELFG
                     YEKGAFTGALSRQIGLFEQANGSTLFLDEIGDLTQSTQAKLLRALQEKEIRRIGGKET
                     IKLDVRIIAATNKKLEKEIEAGRFREDLYYRLNVIAFTLPPLRERRTDIPLLVEHFLK
                     KLDGIHGGRKAVSDEALACLMQYSWPGNVRQLESVLERSYIMCEAGTIGTEYVPAEVR
                     HYSQPRAAIDGITSRLDELINAKLTAAEYRLYLYLTKLDPRDGASDHLLEPKEIAERL
                     GISRDTFFVGVSKLKELGLYDYKARVNELKHPPGRSPRKE"
     gene            complement(1702..3243)
                     /locus_tag="PKJ17_03500"
     CDS             complement(1702..3243)
                     /locus_tag="PKJ17_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75066.1"
                     /translation="MSTSSTDTKDSGRMEVGGRRGMTGKAGKVYALVKSVFRSLSLRT
                     QLLLILLFLLLISIGSLTIIYTRAEEALLDKVGENIDDITRAIQISVEELTYRGDSTA
                     RLKSYVDMLNKKGIKEISIVSEKSEVIASSDPKKIGTTQKLTDKKPAARKKDLMITAR
                     LGDEHRSGTQRLYNIIMPVAIKGQNIGYIHINMALDDYRLLQNRNQIKRILSMIFAFT
                     IGIIVCLVIAEKYTEPIKRIAKASRKIAMGELVKIRETDRKDEIGTLVTSFNEMVDKL
                     DERKELEEKLKKSEQLSLIGQLSSGIAHEIRNPLNFLTLSIGHIKERVTEEPISDKEE
                     LVGLLDSLLKEIYRVNELIHNFLFLGKSITLVREDVPPANLVDEALVMVKDKIRSGID
                     IELSCSDGAGTMYCDREYMRLCVINLVINAVQAIQGPGRVRIECGTEDSVTYLSVSDD
                     GEGIARDELDKIFEPYYSTKKLGIGLGLAITKRFVEEHGGTISAASEIGKGTTMTIRL
                     PRHEG"
     gene            complement(3212..>4168)
                     /locus_tag="PKJ17_03505"
     CDS             complement(3212..>4168)
                     /locus_tag="PKJ17_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOD75067.1"
                     /translation="DEGRPFTLRFRSDGRPITFRDGIRGELSFPRGHVDDLIILRRDG
                     TPTYNLAVVVDDMLMGITHVIRGGDHISNTPKQIALINALGGKPPEYAHHSLLIGPDR
                     KPLSKRHGATGVTDFRAMGIVSEALVNYLSTMGRSVAVELMDPDELVRTFSLDSVSGS
                     DNIFDMEKLLWFNREHMKRMTLSHLADRAGIDASRRERVSVVRENAATLTELREYMEM
                     FDRVEFRPEALRYLMKTDAAGGVAKTVRSRLAAGDSLTFDELTAVLGSPPGLKKRDLF
                     MILRALITGRSDGPPLRDVFPLIPAEVIAGRIDVYLEHGHEG"
BASE COUNT          752 a         1235 c         1190 g          991 t
ORIGIN      
        1 taacgaaggc aacggtgatc aggatcgcga gaacgatcat caatgctttc ttcatgctat
       61 tttcacctcc tccccttttg ttggtcacat tgagttgcat ggggactgtg tatgcagttg
      121 tcgtgccact atcgcaacag attgatatta aaggaaagag gtttggagtg tctttcgggg
      181 aagcggcgat aatcggaaat atccgactcc ggaatgccct ttgaatcgga aatatccgac
      241 tattccttcc tgggggaccg gcccgggggg tgcttgagtt cgttcaccct ggctttgtag
      301 tcgtagagcc cgagctcctt gagtttcgac acgccgacga aaaaggtgtc ccggcttata
      361 cccaggcgct ccgctatctc tttcggctcg aggagatggt cggacgctcc gtctcgggga
      421 tccagcttgg tgaggtacag gtagaggcga tactcggccg ccgtcagctt cgcattgata
      481 agctcgtcga gcctcgacgt tatcccgtcg atggccgcgc ggggctgcga gtaatgcctc
      541 acctcggcgg gaacatactc cgtgccgatg gtccccgcct cgcacattat ataggaacgt
      601 tccagcaccg attccagttg gcggacgtta cccggccagg aatactgcat gaggcacgcg
      661 agggcctcgt cgctcacagc cttcctgccc ccgtgaatac cgtcgagctt cttcaggaaa
      721 tgctccacga gaagaggtat gtcagtccgc ctttccctga gcggcggcag tgtaaaggcg
      781 atgacattga gcctgtaata gagatcttcc ctgaaccttc ctgcctcgat ctccttctcc
      841 agcttcttgt tggtcgccgc gatgatgcgc acatcgagct tgatcgtctc ttttccgccg
      901 atcctccgta tctccttctc ctgaagggcc ctcaagagct tcgcctgtgt gctctgggtg
      961 aggtctccta tctcgtcgag aaagagggtg ctgccgttgg cctgctcgaa gagccctatc
     1021 tgtcgcgaca gggccccggt gaatgcgcct ttctcgtatc cgaagagctc actctcaaga
     1081 agggactccg gtatggccgc gcagttgatg gcgaaaaagg gtttcccctt cctggggctg
     1141 ttatagtgaa tgctcttggc gaaaagctcc ttgcccgttc cgctttcacc gtggacgaga
     1201 acggtggagt tcgtcggggc caccctcctg acgatcttgt aaagctcctc catggcaccg
     1261 tgcgcgccga cgatgttgtc gatgtgaaag cggtcccgca gctggtcctt cagcatgatg
     1321 ttgtccttga ggagcttcac ctggttcatg gcattcgtga tgacgaggag aagttggtcc
     1381 ttctcgaggg gcttctcgag gtagtagaag gcgccaagct tcgtcgcctc cactgccgag
     1441 ggtatggagc cgaaggcggt catgatgatc acgtggcagg tcctccccag cgccctcacc
     1501 tccctcagca cctccgtgcc gtcgatgtcg gggagcttga ggtccgtgag aacgacatcg
     1561 aaactgccgt tcttgaggat atcaaccgct ttctttccac actcggcggc ctcgacgtag
     1621 aaaccctctt tcgagaggat cgtctttatg atcccgcgct ggttcctgtc gtcctcgacg
     1681 acaaggatgg tgcccttcgt gtcatccttc atggcgtgga agccttatgg tcattgtcgt
     1741 tccctttccg atctcgctcg ctgccgatat ggtcccgcca tgctcttcga cgaaccgctt
     1801 cgttatggcg agaccgaggc caatgcccag tttttttgta gagtaatagg gctcgaagat
     1861 cttgtcaagc tcatccctcg cgataccctc cccgtcatcg gagaccgaaa ggtacgtcac
     1921 ggaatcctcc gtcccgcact cgatgcgcac ccttccgggc ccctgtatgg cctgaacggc
     1981 gtttatcacg aggttgatga cgcagagccg catgtattcc ctgtcgcagt acattgtgcc
     2041 ggcgccgtcg ctgcaggaaa gctcgatgtc gatgccgctc cgtatcttgt ccttcaccat
     2101 gacgagcgcc tcgtcgacaa ggttcgccgg gggcacatcc tccctcacga gcgtgatcga
     2161 tttcccgagg aagaggaagt tgtggatgag ctcgttcacc cggtatatct ccttgagaag
     2221 actgtcgaga agcccgacaa gctcttcctt gtccgaaatg ggctcctccg tcaccctttc
     2281 cttgatgtgc cctatggaga gggtcaggaa attgaggggg ttccgtatct cgtgggcgat
     2341 gcccgaggag agctggccga tgagcgaaag ctgctccgat ttcttcagct tctcttccag
     2401 ttccttgcgc tcgtcgagct tgtccaccat ctcgttgaag ctcgtcacca gggtgccgat
     2461 ctcatccttg cggtccgtct cccttatctt gacaagctct cccatggcga tcttcctgct
     2521 tgccttggcg atcctcttga tcggttccgt gtatttctca gctatcacga ggcacacgat
     2581 gatgccgatg gtgaaggcga agatcatgct gaggatcctc ttgatctggt tgcggttctg
     2641 cagaagacgg tagtcgtcga gcgccatgtt gatatggatg tagccgatgt tctggccctt
     2701 gatcgcgaca ggcatgatga tgttgtagag ccgctgtgtg ccgctgcgat gctcgtcccc
     2761 gaggcgcgcc gtgatcataa ggtccttctt ccgcgcggcg ggcttcttgt ccgtcagttt
     2821 ctgggtggtg ccgatcttct tcgggtcgga actggcgatg acctccgact tctcgctgac
     2881 gatggagatc tccttgatcc ccttcttgtt gagcatatcc acatagctct tgagccgggc
     2941 cgtggaatca ccccggtatg tcagctcctc gacgcttatc tggatggccc tggtgatgtc
     3001 gtcgatgttt tcgcccacct tgtcgagaag cgcctcttcg gccctcgtgt agatgatggt
     3061 caacgagccg atggatatga gaaggagaaa cagaaggatg agaaggagct gcgtcctcag
     3121 cgacaggctg cgaaaaacgg atttcacgag tgcgtagact ttccccgcct ttcccgtcat
     3181 gccgcgcctg ccgcccactt ccatgcgccc gctatccttc gtgtccgtgc tcgaggtaga
     3241 catcgatgcg acctgcaatg acctccgcgg gaatgagagg gaagacgtcc cgcaggggag
     3301 gcccgtcaga ccttcccgtg atgagagccc tcaggatcat gaagaggtcc cttttcttca
     3361 ggccgggggg tgacccgagg acggcggtga gctcgtcgaa ggtcagactg tccccggccg
     3421 cgaggcggga ccgaacggtc tttgcgaccc ctccggccgc gtccgtcttc atgaggtaac
     3481 gaagcgcttc cggcctgaac tccaccctgt cgaacatctc catgtattct ctgagctcgg
     3541 tgagcgtggc cgcgttctcg cgcacgacgg acaccctctc cctacgggag gcatcgatcc
     3601 ccgccctgtc ggcgaggtgg gaaagcgtca tgcgcttcat atgctccctg ttgaaccaga
     3661 gaagcttctc catatcgaaa atattgtccg aaccggagac ggagtcaagg gaaaaggtcc
     3721 ggacaagctc gtccgggtcc atcagctcca ccgcgacgct ccgacccatc gtcgacaggt
     3781 agttcacaag cgcctcgctc actatcccca tggcacggaa atccgtaacg cccgtagcac
     3841 cgtgcctctt gctgaggggt ttgcggtcag gacctatgag gagagagtga tgtgcgtact
     3901 ccggcggctt gccccccagg gcgttgatga gggctatctg cttgggcgtg ttggagatgt
     3961 ggtcccctcc ccgtatgacg tgggtgatcc ccatgagcat gtcgtcgacg acgacggcca
     4021 ggttgtacgt cggtgtcccg tcccttctga ggattatgag gtcatccacg tgtcccctcg
     4081 ggaaagaaag ctccccccgt atgccgtccc ggaacgttat cggccttcca tccgaccgga
     4141 agcgcagggt gaagggccgc ccctcgtc
//
LOCUS       DAOPOA010000113         4164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_23184_length_4164_cov_3.052324, whole genome shotgun sequence.
ACCESSION   DAOPOA010000113 DAOPOA010000000
VERSION     DAOPOA010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23184_length_4164_cov_3.052324"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PKJ17_03510"
     CDS             <1..1089
                     /locus_tag="PKJ17_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD75068.1"
                     /translation="AAGVFTPAVRVDAVSVQHYYPAQSFTLLNASGYVVPQRKAAVAS
                     KVTARLEELFVEEGSRVKKDQVLARLENADLAAALRQAEANLNAARASVDQARAELHD
                     ARVNFEREKRLLAKEFTTRSVYDAAEARYKKAQASVAGAESSVRAQQAAVREARVSLE
                     YTYIRAPFDGVVLTKNADVGDIITPIGASSTAKAAVVTLADMSSLKVEADVSESNLGK
                     LKVGQPCQIQLDAIPDTRFDGTVHMIVPTADRTKATVMVKVNFNHLDPRILPEMSAKV
                     AFLSREVGGDERRPRTAVSGKAVVERKGGKYLFVVKDGRADMREVTTGEVFGDMVEIK
                     SGASSGDRVVLDPPERLKDGSRVKITGQ"
     gene            1090..1791
                     /locus_tag="PKJ17_03515"
     CDS             1090..1791
                     /locus_tag="PKJ17_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD75069.1"
                     /translation="MDAGTIVSIQKLCKSYHRGNQVIPVLSDISFDIAEGEFLALMGP
                     SGSGKSTLLNLIAGIDQPDSGSIVIGDVDVTTLAETELAKWRALNVGFIFQFYNLIPV
                     LTAFENVELPLLLTPLSRSERKAHVEMALRVVGLADRMDHYPSQLSGGQEQRVAIARA
                     IVTDPTILVADEPTGDLDRNSAEEIMDLMDRLNEESKKTIIMVTHDPRAAKKAHVIRH
                     LDKGILTNADPQDTL"
     gene            1766..2923
                     /locus_tag="PKJ17_03520"
     CDS             1766..2923
                     /locus_tag="PKJ17_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD75070.1"
                     /translation="MQILKILFKNAFRHKLRTGLTVLSITIALLAFGLLRTVINAWYS
                     GVEASSAYRLVTRNAVSIMFPLPYSYKDRIRQVDGVSEVSWGNWFGGIYIDEKNFFAN
                     FAVDARTYFDLYREYAIPDDERTAFFRDRKGFVAGRKLAERFGWKIGDTVVLKGTIYP
                     GNWEFVMRGIYRGREPNADETVFMFHWDYLNESLKKTAPRRADQVGFYMVGIEDPGRA
                     ISIAEAIDALFKNSFAETITETEKAFTLGFVAMSDTIITAIQLVSFVVIVIILAVVAN
                     TVAMSVRERTNEYAVFKTLGFGGWRIVGLILGESVVITTMGCAVGIAATYPAAALFGK
                     AMGAYFPVFNVEPDTIVLDIVFSLAVGVLAAVIPVYRTLKMKIADGLRMIE"
     gene            2920..4089
                     /locus_tag="PKJ17_03525"
     CDS             2920..4089
                     /locus_tag="PKJ17_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD75071.1"
                     /translation="MKGVPFSYSLRNLWTRRLTTVLTVSGMALVVFVFTTILMLAEGL
                     QKTLVETGSYDNAVIIRKGSGTEVQSWVDRAQAGIIETDPIVATGPGGKRLVAKELVV
                     LIGLPKKDTNKVSNVTIRGIGESSLALRPQVALVRGRMPRMGSSEVVVGSSVAKRFKN
                     ADLGGELRFGMRQWRIVGVFDAGNTGFTSEIWGDVEQLMPAFRRPVFSSMLLRMNDPS
                     RFDDFKKRIETDPRLTQEVKRETRYYLEQSEVMARFLRILGISLTLIFSLGAIIGAMI
                     TMYAAVANRIGEIGTLRALGFRRRSILLSFLVEALLLGLIGGAVGVTFASLMQLVEVS
                     TVNFQTFSELAFKFSLTPGITMRALVFAIVMGLAGGFLPALRAAHMNIVEALRSK"
BASE COUNT          827 a         1253 c         1297 g          787 t
ORIGIN      
        1 gcggccgggg tcttcacacc cgccgtcagg gtcgacgccg tcagcgtgca gcactactat
       61 cccgcccagt ccttcaccct cctcaatgcc agcggttacg tcgttcccca gcgaaaggcg
      121 gcggtcgcct cgaaggtcac ggcccgcctg gaggagctct tcgtggaaga gggcagcagg
      181 gtgaagaagg accaggtcct ggcgcgtctc gagaatgccg accttgccgc cgcgttgagg
      241 caggcagagg ccaatctcaa tgcggcgcgc gcatcggtag atcaggcacg ggccgaactg
      301 cacgatgcca gggtgaactt cgaacgggag aagcgcctcc ttgccaaaga gtttaccacc
      361 cgctccgtct atgacgccgc cgaggccagg tacaagaagg cccaggcttc cgtcgcgggc
      421 gccgaatcct cggtgagggc ccaacaggcc gctgtcaggg aggcccgggt ctctctcgaa
      481 tacacgtaca tacgggcgcc ttttgacggt gtggtgctca cgaagaatgc cgatgtcggg
      541 gacatcatca cccccatagg cgcctcgtcc accgccaagg cggcggtcgt caccctcgcg
      601 gacatgtcgt cgctgaaggt ggaggcggac gtttccgagt cgaacctggg aaaactgaag
      661 gtcggacagc cctgccagat ccagctcgac gcaattcccg atacgcgttt cgatggcacc
      721 gtccacatga tcgtccccac ggcggacagg acaaaggcga cggtcatggt gaaggtgaac
      781 ttcaaccacc tcgatccgag gatactcccc gagatgagcg cgaaggtcgc ctttctgtca
      841 cgggaggtcg gaggcgacga gaggagaccg cggacggcgg tgagcggcaa ggccgtcgtc
      901 gagaggaagg gcgggaaata tctcttcgtc gtgaaggatg ggcgcgccga catgagagaa
      961 gtgacaacgg gagaggtctt cggcgacatg gtggagatca aaagcggcgc ctcctcgggc
     1021 gacagggttg tcctcgatcc ccccgagcgc ctcaaggacg gctcccgggt gaagataaca
     1081 gggcagtaga tggacgccgg gacgatagtc tccatacaaa agctctgcaa gtcctatcac
     1141 cgggggaacc aggtcatccc ggtcttgagc gacatcagct tcgacatcgc cgaaggggag
     1201 ttcctggcgc tcatgggtcc ctcgggttcg ggcaagagca cgctcctcaa tctcattgcc
     1261 ggcatcgacc agcccgacag cggttccatc gtgatagggg acgtcgacgt cacgacgctc
     1321 gcggaaacgg agctggcgaa atggcgtgct ctcaacgtgg gtttcatctt ccagttctac
     1381 aacctcatcc ccgtgctgac ggccttcgag aacgtcgaac tgcccctcct gctgacaccc
     1441 ctttcacgtt ccgagcgcaa ggcgcatgtc gagatggcct tgagggtggt tggacttgcc
     1501 gaccggatgg accactaccc gtcgcaactc tcgggcgggc aggagcagcg cgtcgcgata
     1561 gcgagggcga tcgtcaccga ccccaccata ctcgttgccg acgaaccaac gggggacctc
     1621 gacaggaact ccgccgagga gatcatggac ctcatggacc gattgaacga ggagtcgaag
     1681 aagaccatca tcatggtcac ccacgatccg cgggcggcga agaaggcgca cgtcatcagg
     1741 cacctcgaca agggcatcct gaccaatgca gatcctcaag atactcttta agaacgcctt
     1801 caggcacaag ctccgcaccg gtcttaccgt tctttccatc accatcgccc tccttgcgtt
     1861 cgggctcctg aggaccgtca tcaacgcctg gtattcgggg gtcgaggcct cgagcgccta
     1921 caggctcgtg acgcgcaacg cggtctccat catgttcccc cttccctatt cttacaagga
     1981 caggatcagg caggtggacg gagtctcgga ggtgtcctgg gggaactggt tcggcgggat
     2041 ctatatcgac gagaagaact tctttgccaa ttttgcggtc gacgcaagga cgtatttcga
     2101 cctctaccgt gagtatgcta tccccgatga cgagaggacg gccttcttcc gggaccgcaa
     2161 ggggttcgtc gcgggacgga aacttgcgga gagattcgga tggaagatag gggacaccgt
     2221 cgtcctcaag ggcaccatct atcccggcaa ctgggaattc gttatgcgcg gcatatacag
     2281 ggggagggag ccgaacgcgg acgagacggt cttcatgttc cactgggact acctcaacga
     2341 gtccctgaag aagaccgccc cgaggagggc cgaccaggtg ggcttctaca tggtgggtat
     2401 cgaggacccc ggccgggcga tctccatcgc cgaggccatc gacgcccttt tcaagaactc
     2461 cttcgccgag accatcacgg aaacggaaaa ggccttcaca ctgggtttcg tggccatgtc
     2521 ggacacgatc ataacggcga tccagctcgt ttcctttgtc gtcatcgtca tcatccttgc
     2581 cgtcgtcgcg aacacggtgg ccatgtcggt cagggagcgc acgaacgagt acgccgtttt
     2641 caagacgctg ggtttcggcg gctggcggat agtgggactt atcctgggag agtccgtggt
     2701 catcacaacg atgggatgcg ccgtggggat cgcggccacg taccccgcgg ctgcgttatt
     2761 cggcaaggcg atgggggcct acttccccgt tttcaacgtc gaacccgata ccattgtcct
     2821 cgatatcgtt ttttccctgg ctgtgggcgt cctcgcggcc gtgatacccg tataccgcac
     2881 cctgaagatg aagatcgccg acgggttgag gatgatcgaa tgaaaggcgt tcccttttcc
     2941 tacagcttga ggaacctctg gacccgcagg ctcacgacgg tgctgaccgt ctcgggcatg
     3001 gccctcgtcg ttttcgtgtt cacgacgatc ctcatgctcg ccgagggcct ccagaagacc
     3061 ctggtcgaaa ccgggtcgta cgataatgcc gtcatcatac ggaaggggtc gggtacggag
     3121 gtgcagagct gggtggatcg tgcccaggcg ggcatcatcg aaacggatcc catcgtcgca
     3181 acggggcccg gtggaaagcg actcgtggcg aaggaactcg ttgtcctcat cggtcttccc
     3241 aagaaggaca cgaacaaggt ctccaacgtg acgatccggg ggatcgggga aagctccctt
     3301 gcccttcgtc cccaggtggc cctcgtgagg ggccggatgc cccggatggg ctcctcggag
     3361 gtcgtcgtcg gctcaagcgt cgcgaaacgc ttcaagaacg ctgacctggg aggcgagctg
     3421 cgcttcggga tgcggcaatg gcgcatcgta ggggttttcg acgcgggcaa cacgggtttc
     3481 acctccgaga tatgggggga cgtggaacaa ctcatgcccg ccttcagacg gcccgttttc
     3541 tcctccatgc tccttcggat gaacgacccc tcgcggttcg acgacttcaa gaaaaggatc
     3601 gagacggacc cccgtctcac acaggaggtg aaacgggaga cccgctacta cctggaacag
     3661 tcagaggtga tggccaggtt cctgcgcatc ctcggaatat cgctgacgct catcttctcc
     3721 ctcggggcca tcataggggc catgatcacc atgtacgccg ccgtggcgaa caggataggc
     3781 gagatcggca ccttgcgggc gctcggtttc cgccgcagga gcatcctcct gtccttcctg
     3841 gtggaggccc ttctcctcgg cctcatcggc ggggcggtgg gagtaacgtt cgcctctcta
     3901 atgcagctcg tggaggtgtc cacggtgaac ttccagacct tctcggagct tgccttcaag
     3961 ttctccctca cccccggcat cacgatgagg gcccttgtct tcgcgatcgt catgggcctt
     4021 gcgggaggct tcctgccggc cctgagggcg gcgcatatga acatcgtgga ggcgttgaga
     4081 agcaaatgaa gacggttcca ggttcagggt ttcagatttc aagcaatacg tagttgcggg
     4141 ttccgagttg cgcgtttcga gtca
//
LOCUS       DAOPOA010000114         4138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_23392_length_4138_cov_3.519226, whole genome shotgun sequence.
ACCESSION   DAOPOA010000114 DAOPOA010000000
VERSION     DAOPOA010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23392_length_4138_cov_3.519226"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..527
                     /locus_tag="PKJ17_03530"
     CDS             60..527
                     /locus_tag="PKJ17_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD75072.1"
                     /translation="MGTKDLSAIIELAISKEQEAYDFYTELLSMVDDRAARDALQIVA
                     GEEKKHKEFLVNYRDKGYGDSGLKMTTVVDYKIAEHLEAPEPRAGMESKDVFLIAAHR
                     EKGAHEFYLGLASIHPAGPTKDMLLQMAGEELKHKEKMEYYYANAAFPQTSGG"
     gene            585..1103
                     /locus_tag="PKJ17_03535"
     CDS             585..1103
                     /locus_tag="PKJ17_03535"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOD75073.1"
                     /translation="MGFIEFAIFDSWFGTISVVRRERRIVRLDLSENDIYQEHRAVLK
                     RFPDAVESEQSFKTVRTLLHRYLKGQPVDFRDVEIDISDLPEFTQTVLEELRKIPYGE
                     TRSYLDIARRAGHPLGGRAVGQAVKRNPIPIIVPCHRVIRHDGSLGGFGLGEKIKKRL
                     LFLEGVKKTWSV"
     gene            complement(1038..1568)
                     /locus_tag="PKJ17_03540"
     CDS             complement(1038..1568)
                     /locus_tag="PKJ17_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75074.1"
                     /translation="MADRLRTLRRLVPVALCLLSVLLLSSCGDLQAKGIWSAIAGTRQ
                     DDVWYIDQTLAYSTHDKVTTSRAVLKFVPGQGSVLNEEIRECLDLNGIVPKDLSYFVA
                     FVSVDCRRELLHFSDMAFFDSEDNVIWRQQYEETYHIVPTADTSAGVISGYLCLNRPG
                     FLDTLKKKKPFLYLFP"
     gene            complement(1660..>4138)
                     /gene="uvrA"
                     /locus_tag="PKJ17_03545"
     CDS             complement(1660..>4138)
                     /gene="uvrA"
                     /locus_tag="PKJ17_03545"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOD75075.1"
                     /translation="IVDAILGLGKGENLTILAPIVRGRKGEYRKEIDELRQHGFTKVR
                     IDGAVMDLADDIELDKNKKHEIEVVVDRITIREGVERRLFEAVELALSRAGGLVKVET
                     GSQARVFSEKFACPDCGISYPEIAPRMFSFNNPYGACPACTGLGVKEYFDPALIVPNQ
                     DLSLRDGAVVPWAEKNPVHFLPFLEALVKHYRIDIRKPFRSLPKDVREAILYGSKGEE
                     IEFFHDHGGRRSFSRRPFEGVIGELTRDYEDADFYEREKLERFINLIPCPECNGARLR
                     KEMLWVRVGGLNIFEVSRMTIEECATFFDAIELPEKEQEIARTILKEVTSRLKFLLDV
                     GLDYISLSRNSATLSSGESQRIRLATQIGSGLTGVLYVLDEPSIGLHQRDNDRLLQTL
                     RRLRDLDNTVIVVEHDYDAIVSSDYVVDLGPGAGEKGGHLVFQGTPAELKMHPESVTG
                     LYVSGRRSIERPAGRRQPRGSLTVKGARANNLKNIDVAIPLGVFTAVTGVSGSGKSTL
                     VVDTLYPALKQKLYRSKERVGESDGILGFEAVDRVIDIDQSPIGRTPRSNPATYTGVF
                     THIRELFTRLPESRMRGYKEGRFSFNVKGGRCETCAGEGFVKIEMQFLPDVYIVCEAC
                     KGKRYNRDTLEILYRGKSIADVLEMTVTQAMEFFDAHPHIKSKLKVLYEVGLGYIRLG
                     QAATTLSGGEAQRIKLSRELSKRDTGKTFYILDEPTTGLHFVDIEKLLHVLNELVERG
                     NTVVVIEHNLDVIKSADYVIDLGPEGGGRGGTIIARGTPEDVMSAENSYTGLFLKRYL
                     EADRGSAAGERVGAKSASGSRSSRVPG"
BASE COUNT          855 a         1222 c         1214 g          847 t
ORIGIN      
        1 gaccttgcgc gcccgggcgg agtgtgccaa tatataagtg caagcgaaaa ggagggttta
       61 tgggaacaaa agatctctcg gccatcatcg agttggcgat atcaaaagag caggaagcct
      121 acgatttcta cacggaactt ctgagcatgg tcgacgacag ggccgccaga gacgccctgc
      181 agatcgttgc cggcgaggaa aagaaacaca aggagttcct cgtgaattac cgcgacaagg
      241 gctacgggga ctcgggcctc aaaatgacaa ccgtcgtcga ttacaagata gccgaacacc
      301 ttgaggcccc cgagcccagg gccggtatgg aaagcaagga tgtcttcctt atagcggccc
      361 atcgggaaaa gggcgcccac gagttctacc tgggacttgc ctccattcac cctgcggggc
      421 caacgaagga catgctcctg caaatggccg gggaagaatt gaagcacaag gagaaaatgg
      481 agtattatta cgcgaacgcc gctttcccgc agacatccgg cgggtgacag cacgagggga
      541 tacatctctc ctgagagcgc cgcgtgcaca ccgcgaggaa gtctttgggg ttcattgaat
      601 ttgccatatt cgactcctgg ttcggaacca tctccgttgt aaggagagag cgccggatcg
      661 ttcgtctcga tctttcggag aacgacatat accaggagca ccgggccgta ctgaaacgtt
      721 ttcccgatgc tgtcgagtcg gagcagtcct tcaagacagt gcgcaccctt ctccaccggt
      781 acctgaaagg acagccggtg gatttccgtg atgtggagat cgatatatcg gacctgccgg
      841 agttcacgca gacggtcctg gaagagctgc gaaagatccc ctacggcgaa acgagaagct
      901 atctcgatat cgcgagacgc gccggacacc ccttgggggg acgggccgtc ggtcaggcgg
      961 tgaagaggaa ccctatcccc atcatcgtcc cctgccacag ggtcatccgc cacgacgggt
     1021 ccctgggggg attcggtcta ggggaaaaga taaagaaaag gcttcttttt cttgagggtg
     1081 tcaagaaaac ctggtcggtt taggcagaga tagccggaga tgacgccggc ggacgtatcc
     1141 gccgtgggca cgatatgata cgtctcttcg tactgctgcc gccagatcac attgtcctcg
     1201 gaatcgaaga aggccatatc tgaaaaatgg agcagttccc tccggcagtc gacggaaaca
     1261 aacgctacga aataggaaag gtccttcggg acgatcccgt tgaggtcaag gcattccctg
     1321 atctcctcat tgaggacgct tccctgaccg gggacgaatt tcagcacggc cctcgaagtg
     1381 gtcactttgt catgggtcga gtatgcgagg gtctgatcga tataccagac atcatcctgc
     1441 ctggtcccgg caatggccga ccatatcccc ttcgcctgca gatctccgca tgaagacaac
     1501 agcagcaccg acaaaaggca gagcgcgacg gggacaagcc gccgcagtgt tcgcagtcga
     1561 tcggccatat ttcgtttatc gcacctttgc cgtggagcct aaacagactg ttgacaaagt
     1621 gacttcccag ggcggttcgt gctctttcgt cgggtgcact taccctggaa ccctagaact
     1681 ccgagatcct gaagcgcttt tcgcccccac cctctccccg gccgccgatc cccggtcggc
     1741 ctcaagatac cgcttcagaa acagcccggt gtaactattc tctgcggaca tgacgtcttc
     1801 cggggtgcca cgggcgatga tcgttcctcc ccggccgccc ccctcgggac cgaggtcgat
     1861 cacataatcg gcactcttga taacgtcgag attgtgctcg atgacgacga cggtgttgcc
     1921 cctctcgacg agttcgttga gcacgtgaag gagcttctcg atgtcgacga aatggaggcc
     1981 tgtcgtcggt tcgtcgagaa tatagaaggt cttacccgtg tcccgtttgg agagttcacg
     2041 ggagagtttg atcctctgtg cctctccgcc ggatagggta gtcgccgcct gacccaggcg
     2101 gatatacccc agaccaacct cgtagaggac cttgagcttg ctcttgatgt ggggatgggc
     2161 gtcgaagaac tccattgcct gcgtgaccgt catttccagg acatctgcga tactcttgcc
     2221 gcggtagagg atctccagcg tgtcgcggtt gtaacgcttc cctttgcacg cctcgcagac
     2281 tatgtaaaca tcggggagaa actgcatctc tatcttcacg aagccctctc cggcgcaggt
     2341 ctcgcacctg ccccccttca cgttgaagct gaaccttccc tccttgtatc ccctcatccg
     2401 cgactccgga aggcgcgtga agagctcccg gatatgggtg aaaacgccgg tgtaggtggc
     2461 ggggttggac ctcggggtcc gcccgatggg cgactggtcg atatcgatga cgcggtcaac
     2521 ggcttcgaac ccgagtatac cgtcggattc gccaacgcgc tcctttgatc tgtacagttt
     2581 ctgtttcaat gcgggataga gtgtatcgac gacgagggtg ctcttgcctg accccgatac
     2641 gccggtgacg gcggtgaaga caccgagggg tatggcgacg tcgatgttct tcaggttgtt
     2701 cgcccgcgcc cccttgaccg tcagagatcc ccgtggctgc ctcctgccgg ccggcctttc
     2761 gatagagcga cgtcccgaca cgtacaggcc cgtcaccgat tcaggatgca tcttcagttc
     2821 cgcgggcgtt ccctggaaca cgaggtgacc gcccttctca ccggccccgg ggccgaggtc
     2881 gacaacgtaa tcggaagaga cgatcgcgtc gtagtcgtgc tcgacgacaa taacggtatt
     2941 gtcgagatcg cgaagccgac gcagcgtttg caggagcctg tcgttgtccc tctggtggag
     3001 cccgatgctg ggttcatcga gaacgtagag cacccccgtc aggcccgagc ctatctgcgt
     3061 ggcaaggcgg atcctctgcg attcaccgga cgagagggtc gcggagttgc ggctgagact
     3121 gatatagtcc agaccgacgt cgagaaggaa cttgagccgt gatgtgacct ccttgagtat
     3181 cgtccgggcg atctcctgct ccttctcggg aagctcgatc gcgtcaaaaa aggtggcgca
     3241 ttcctcgatc gtcatccggg atacctcaaa gatattgagg ccgcccaccc tgacccagag
     3301 catctccttc ctgagccggg caccgttaca ctccggacag gggatgaggt tgatgaaccg
     3361 ctccagcttc tcccgttcgt agaagtcggc gtcctcataa tcccgggtaa gctccccgat
     3421 gaccccctcg aagggcctcc ggctgaagct cctgcgcccg ccgtggtcgt ggaagaactc
     3481 tatctcctcg cctttcgagc catacaggat ggcctcccgc acgtccttcg ggaggctcct
     3541 gaagggtttc ctgatatcga tcctgtaatg cttgacgagg gcctcgagaa aggggaggaa
     3601 gtggacgggg ttcttttcgg cccagggcac gaccgcgccg tcacggagcg aaaggtcctg
     3661 gttaggaacg atcagggcgg ggtcgaagta ctccttgacg cccagccccg tgcacgccgg
     3721 gcacgccccg tagggattgt tgaaagagaa catccgcggg gctatctccg ggtagctgat
     3781 gccgcagtcg ggacaggcaa acttctcgct gaaaacgcgc gcctgactgc cggtctcgac
     3841 cttcacgagg ccgcctgccc gggaaagggc cagctccacg gcctcaaaaa gccgccgttc
     3901 cacgccctcc cggatggtga tgcggtcgac gaccacctcg atctcatgct ttttgttctt
     3961 gtcgagctcg atatcgtcgg caaggtccat gacggcgccg tcgatcctga ccttggtgaa
     4021 accgtgctgc ctgagctcgt cgatctcctt gcggtattcc ccttttcggc cccggacgat
     4081 cggggcaagg atggtgaggt tctctccttt gccgagaccg agaatggcgt cgacgatc
//
LOCUS       DAOPOA010000115         4105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_23627_length_4105_cov_3.070123, whole genome shotgun sequence.
ACCESSION   DAOPOA010000115 DAOPOA010000000
VERSION     DAOPOA010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23627_length_4105_cov_3.070123"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PKJ17_03550"
     CDS             complement(<1..630)
                     /locus_tag="PKJ17_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOD75076.1"
                     /translation="MKTSDVLVRQLEEEGVEHIFGLPGEENLSFLESLRTSRIRVHIT
                     RHEQAAAFRAATYGRLTGKAGICFSTLGPGATNLVTGIAHAQLVGAPLIAITGQKALR
                     DNRQARFQLIDVVSLMSPITKYSTSIADSGTVPTVVRNAFKLAQAERPGPVHIELPED
                     VAESETDAALQLPGASRRPAPDVKAVALAAGLINGAHHPLILLSGGANRN"
     gene            complement(643..>2013)
                     /locus_tag="PKJ17_03555"
     CDS             complement(643..>2013)
                     /locus_tag="PKJ17_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOD75077.1"
                     /translation="PLGKMQSINPYTEEIIEEFPLMTKTELNEAVEACRAAFHTWRHA
                     PVEERAAHVKRMGAHLRDEKRKYAELITREMGKPIREALAEIEKCAWLCDHFSENAAL
                     LLMPEEIKTEAYRSYVAFEPLGLVLAIMPWNFPFWQVFRFGIPAICAGNCVVLKHAPS
                     VPATALAIEEAFMTVGFPQGVLRTVLVDPPEALALLDEDKVDAVTLTGSVRAGSQVAQ
                     AAGRNIRNAVLELGGSDPFIVLDDADIEKAARTGAMARVINTGQSCIAAKRFIVLRQV
                     ADEFQKLFVEHLEALKIGDPMDEATDLGPLSRKDVLENLKEQLKDARAKGGEIIQRDL
                     SFQKGFFFAPAVVLHPKETMKVLNEEVFGPVAPIIVVDSEEEAVRAANATEYGLGASI
                     WTRDLSRGQRVAGQIDAGAIAINDFVRSDPRLPFGGVKRSGIGRELSHYGLKEFVNIK
                     SIVVIK"
     assembly_gap    2014..2023
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2054..3292)
                     /locus_tag="PKJ17_03560"
     CDS             complement(2054..3292)
                     /locus_tag="PKJ17_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOD75078.1"
                     /translation="MDDTTEVKTSEGDKKTGKTVRVKNIGLRGVTVADTKISRIDGQN
                     GVLIYRGFRIEELARDSTFEETAYLLHHDELPGREELRKFTELLVEARTMPRFVFDVL
                     KGLPKESHPMDVLQAAVPVLAMADPEPGNETREANMAKAVRLISRIPVIVAGWQRIRQ
                     GASPLPSKDALSHAANFLWQLTGKEPDEGIARDLDIALLLHADHSFNASTFACREVTS
                     TGAHMYASVAAGVGALSGKLHGGANTEVVKMLLELKGEKDIPGWIRNRLETGLKIMGL
                     GHAVYKTLDPRAPFLRDMCFALGEKLHQDDWCQLLARIEQSATAELAKRGKETVRPNV
                     DFWSASVYQFMGIPLDLMTAVFAVARVAGWTAHVIEEKFADAQEKPALYRPLSEYVGH
                     YCGKTGCIYEPIEKRGTAKG"
     gene            complement(3285..>4105)
                     /locus_tag="PKJ17_03565"
     CDS             complement(3285..>4105)
                     /locus_tag="PKJ17_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75079.1"
                     /translation="RNFPGRSGTRDDQVYLCSPETATASALKGVITDPRDLASEMAYP
                     RIRDAERYLTDDRSIIFPSEELKYTVVIRGPNIKPLPELTPLPDSIAAEVVLRVGDNI
                     STDGIMPAGNQVLPYRSNIQEISKHVFEQIRPDFAAACQARGVVVVVGGQNYGQGSSR
                     EHAALAPRYLGVCAKIVKSFARIHKSNLCNFGILPLTLADPEDYPLFEEGTKVIFPDV
                     RKRIERGDREIPVEVSGKSIMTLLDVSQRQREHLLAGGTLNFVKNEFQNRGDHG"
BASE COUNT          795 a         1220 c         1178 g          902 t
ORIGIN      
        1 gttccggttt gcacccccgg agagtaggat aagcgggtgg tgtgcgccgt tgatcagtcc
       61 ggcggcgagg gccacggcct tcacgtccgg ggcgggccgc cgggaagcgc ccggcaattg
      121 taaagccgcg tctgtttcgc tctccgcaac gtcttcgggc aactcgatat gcaccgggcc
      181 cggccgttca gcttgagcca gcttaaaggc attgcgcact actgtaggga ccgtgcctga
      241 gtcggcgatg gaggtggaat acttggtgat cggggacata agactcacga cgtcgatgag
      301 ttgaaagcgt gcctgccgat tgtcgcgaag ggccttctgc cccgtgatgg ctatgagcgg
      361 cgcgccaacg agctgtgcat gggcaatgcc ggtgacaagg ttcgtcgcgc ccgggccgag
      421 ggtcgaaaaa cagatgccgg ctttcccggt cagccgccca tacgtggcgg cccggaaagc
      481 tgcggcctgt tcatggcggg taatatgcac cctgatccgg gaggtgcgca gtgattcgag
      541 gaaggagagg ttctcctcgc cgggcaggcc aaagatatgc tccacgccct cttcttcgag
      601 ttgtctcacc agcacatcgg atgtcttcat gccttcgccc tttcacttga tcacaacaat
      661 gctcttgata ttcacgaatt ctttgaggcc ataatgtgac aactccctgc cgatgccgga
      721 ccgcttcacc ccgccaaagg gcagccgggg atccgatctc acgaagtcat tgatggcaat
      781 agctcctgca tcgatctgcc ccgcgacccg ctggcctctg cttaagtccc tcgtccagat
      841 cgatgccccg agtccgtact ccgtcgcgtt tgcagcccgg acggcctctt cctcgctgtc
      901 caccacgata attggcgcaa caggaccgaa gacctcctcg ttgaggacct tcatagtttc
      961 cttcgggtgg aggaccacgg caggggcgaa gaagaatcct ttttgaaacg aaaggtcccg
     1021 ctggataatc tccccacctt ttgccctggc gtccttaagc tgttccttaa ggttttccag
     1081 gacatccttt cgggaaagag gaccaaggtc ggtggcttca tccatgggat cgccgatctt
     1141 cagcgcttcg aggtgttcca cgaagagctt ttggaactcg tcggccacct gcctgaggac
     1201 gatgaaacgc ttggccgcta tgcagctctg gccggtgttt atcacccggg ccatagctcc
     1261 cgtccgtgcc gctttttcga tgtccgcgtc atcgaggacg atgaaaggat cggaaccgcc
     1321 cagttcaagg acggcattcc tgatgtttct ccccgccgcc tgggccacct gtgacccggc
     1381 cctgacgctg cccgtaaggg taacggcgtc caccttatcc tcatcaagga gcgcgagggc
     1441 ctcgggagga tcgacaagaa ccgtccggag aacaccctga ggaaagccca cggtcatgaa
     1501 tgcttcctcg atggcaaggg cggtggccgg aacggagggt gcgtgcttca ggaccacaca
     1561 attccccgcg cagatcgcgg gtataccaaa ccggaagacc tgccagaacg ggaagttcca
     1621 gggcataatg gccagcacca ggcccagcgg ttcgaaggcg acatagctcc tgtacgcttc
     1681 ggtcttgatc tcctcaggca tgagcaaaag cgctgcgttt tccgaaaagt gatcgcacag
     1741 ccaggcacat ttctcgatct cggccagggc ctcccggatg ggcttgccca tctcccgggt
     1801 gatgagttcc gcatactttc gcttctcgtc acggaggtgg gcgcccatac gcttcacgtg
     1861 cgccgcccgc tcctccacgg gtgcgtgtcg ccaagtgtga aacgcagcgc ggcatgcttc
     1921 caccgcctca ttcaactcgg tttttgtcat gagggggaat tcttctatga tctcctcggt
     1981 ataggggttg atcgactgca tcttccccag gggnnnnnnn nnngagtgtt tgaacgttga
     2041 tcgatcatgt ttgtcatcct ttcgctgtgc ccctcttttc gataggctca tagatacatc
     2101 cggtcttgcc gcagtaatgg cccacatatt ccgacaaagg ccggtagagg gcgggctttt
     2161 cctgtgcatc ggcaaacttc tcctcgataa cgtgggctgt ccagccagca actcgtgcca
     2221 ccgcaaagac cgccgtcata aggtccaggg gaattcccat aaactgatac acggaagcgc
     2281 tccagaaatc aacgttgggc ctgaccgttt cctttcctcg cttagcgagc tcggcggttg
     2341 cagattgctc tatccgtgcc aggagttggc accaatcatc ctgatggagt ttctccccca
     2401 gggcaaagca catatcccgc aaaaacgggg cacggggatc aagggtctta tataccgcgt
     2461 ggccgaggcc catgatcttt aagcccgttt caagccggtt ccttatccac ccgggaatgt
     2521 ccttctcgcc cttaagctcg aggagcatct tcacgacctc agtgttggcc ccgccgtgca
     2581 gcttccccga aagggctcct acacctgccg ccacgctagc atacatgtgg gcccccgtgg
     2641 aggtgacctc acggcacgcg aaggtggacg cattgaagga atggtcggcg tggagcaaga
     2701 gtgcgatgtc gaggtcccgg gcaatcccct cgtcaggttc tttgccggtg agctgccaca
     2761 ggaaattggc ggcgtgggaa agagcatcct tcgacggcag aggtgaagca ccctggcgta
     2821 tcctctgcca accggctacg atcacgggaa tgcggctgat gaggcggacc gccttggcca
     2881 tgtttgcctc tcgcgtttcg ttgcccggtt ccgggtctgc catcgccagg accgggaccg
     2941 ctgcctggag aacgtccatg ggatgagact cttttggcaa ccccttcagc acatcgaaaa
     3001 cgaaccgggg catggtccgt gcttcgacga ggagttccgt gaatttccgg agctcttccc
     3061 tgccggggag ctcatcgtgg tgaagcaggt aggcggtctc ttcaaacgtc gaatcccggg
     3121 ccaattcttc gatccggaaa ccccggtaga tgagaacacc gttttggccg tcgatacgac
     3181 tgatctttgt atcggccacc gtgacgcccc tcaagccgat gttcttcacc ctgacggtct
     3241 ttcccgtttt cttatcccct tcggatgtct ttacctcggt agtgtcatcc atggtctccc
     3301 cggttctgga attcgttttt cacgaaattc aaggtccctc ccgccagaag gtgctcccgc
     3361 tggcgctgtg aaacatcgag gagggtcatg atgctttttc ccgaaacttc gaccgggatc
     3421 tccctgtcac cccgttcgat ccttttgcgc acatcgggga agattacctt cgttccttcc
     3481 tcgaacagag gatagtcttc cgggtctgcc agtgtaaggg gcaagatgcc gaagttgcag
     3541 aggttcgatt tgtggatgcg ggcgaagctc ttcactattt tggcgcacac gccaaggtac
     3601 cggggggcca gagctgcatg ctcgcggctc gatccctggc cgtagttctg acctcccacg
     3661 acaacgacaa cacccctcgc ctggcaggca gcggcaaaat ctggccggat ctgctcgaag
     3721 acatgcttcg agatctcctg tatgttcgaa cgatagggaa gtacctggtt tccggcgggc
     3781 atgatcccat cggtggagat gttatcaccg acccggagaa ctacttcagc cgcaatggaa
     3841 tcgggaagag gcgtaagctc gggcaagggt ttgatgttcg gaccccgtat gaccacggta
     3901 tacttcaatt cttcggaggg aaatatgatg gagcggtcgt cggttagata cctctcggca
     3961 tcccggatgc gcgggtaggc catctctgag gctaagtcgc gtggatcagt gataacaccc
     4021 ttcagggcag aagctgtcgc cgtctccggc gaacagagat agacctggtc atcccttgta
     4081 ccggagcgtc cgggaaagtt tctcg
//
LOCUS       DAOPOA010000116         4101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_23649_length_4101_cov_3.472071, whole genome shotgun sequence.
ACCESSION   DAOPOA010000116 DAOPOA010000000
VERSION     DAOPOA010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23649_length_4101_cov_3.472071"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKJ17_03570"
     CDS             <1..215
                     /locus_tag="PKJ17_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOD75080.1"
                     /translation="LIMGVTQVIQQKMTPTSTDPMQQKMMMFMPIVFTFIFWGFPSGL
                     VLYWLVYNVASIAQQYYINRKTKVAR"
     gene            229..1029
                     /gene="jag"
                     /locus_tag="PKJ17_03575"
     CDS             229..1029
                     /gene="jag"
                     /locus_tag="PKJ17_03575"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOD75081.1"
                     /translation="METLEFEGKTYEEAVKKASQELNVDERDLDIEVKEVDTKGILGL
                     LGTKKIRIMVKLRAKEEGNGEGSTITPTSAETPSGETPLRETAEEFGIAFLKDFGKHV
                     GLDFQVMATDISEKTDRILFLVQCDDGDVLIGKDGEILEALQHLLRLAVAKRFKHNLK
                     LLIDINGYRERRKKALTIMAKRLADKAKRSGKKFKTDPLNPYERRIIHTLFKHNKGIT
                     TKSEGEGHIKRVVLTPTGIPFGGDRGGARGGPRGGARGGFRGNARGRR"
     gene            1004..2386
                     /gene="mnmE"
                     /locus_tag="PKJ17_03580"
     CDS             1004..2386
                     /gene="mnmE"
                     /locus_tag="PKJ17_03580"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOD75082.1"
                     /translation="MATRAGDADTICAVSTPPGEGGISIIRISGLKAHPILEKIFRPR
                     GRKPRKRSYGRLRLGHIVDPADAREVDEVLAVLMAAPATYTREDIAEIHSHGGFAAQK
                     AVMDLVMRQGARLAEPGEFTRRAFLNGRIDLVQAESVLDLIRSETDEELRYALTCLKG
                     GLSAKMGLFRETLKQALVAVEATIDFPDEDLRIDLSGPFASLRKAKKGIDGLVSSYYE
                     GKGLKQGFEVLILGRTNVGKSSLLNALALRERAIVTALPGTTRDLIEETLYLKGAKVR
                     LIDTAGIRTPENVVEEEGIRRVRDKAAEADLIIWVLDGSAPYSDDDQRVLEDIRPYNK
                     IVVANKCDLTQYLEYKLLVSQGISDILHISAQKGIGLDHLRSSLFSALAGKERKNSLL
                     ITTLRHRDILMKVSANITAAIDGLEAGAPPEILAFELQEALHHLGEITGETCPEEILD
                     TIFSQFCIGK"
     gene            complement(2560..3111)
                     /locus_tag="PKJ17_03585"
     CDS             complement(2560..3111)
                     /locus_tag="PKJ17_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75083.1"
                     /translation="MKGRGAFLMGLIAVFIVLAVSITCAAQTAQPQPQPPTASGEQVK
                     KEVPKGEPQWRLYMGNETRRYYFDSNSIERLDKRVVRVWERITQPDKDGNEVEKVQTL
                     MELDCGSSRYRVIAEKEFGSARAGEQPEARMQNGPWTYFEQDVILGILQDNVCFPLST
                     PAGVVPPKPVGSEAPPKPPTFKN"
     gene            complement(3141..>4101)
                     /locus_tag="PKJ17_03590"
     CDS             complement(3141..>4101)
                     /locus_tag="PKJ17_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOD75084.1"
                     /translation="VRSLKVEGALVSLDVNLGYVYAIVGGRDFEKSPYNRATVAKVQA
                     GSAFKPFIYVTALKKGYTPDSVLHDEPKSYSTGMGRSWTPRNYDGKYQGDISMRDAIA
                     YSKNAATVRLLEEIGIGPVKETIRDIGIEADIEDNLSIALGSSNITLLELVKGFAAFA
                     NGGYRIKPIFVRRIVDQDGTVLEENAALKERALPEEVASSMNILLKGPVQYGTAKGAS
                     SIGYPVAGKTGTTSNYYDALFVGYSPHVATGVWVGFDARASLGKGESGGRVCLPIWMS
                     FMGSVLGRFPGGDFPEVSWTAPSGVTGEGGTQYNTNTIEGMGR"
BASE COUNT          945 a         1213 c         1094 g          849 t
ORIGIN      
        1 cgctcatcat gggtgtgacc caggtcatac agcagaaaat gacacccaca agtaccgatc
       61 cgatgcagca gaaaatgatg atgttcatgc ccatcgtctt taccttcatc ttctggggct
      121 tcccgtcggg actcgtacta tactggctcg tctacaacgt cgcctccatc gctcagcagt
      181 attacatcaa caggaagacc aaggtggccc ggtaaggaga aaagacacat ggaaacgctc
      241 gagttcgaag gaaagacata tgaggaggcc gtcaagaagg cctcccagga gttgaatgtc
      301 gatgaaaggg acctcgacat cgaggtaaaa gaggtggaca cgaagggtat tctcggcctc
      361 ctgggcacga aaaagatacg tatcatggtg aagttgaggg caaaggagga gggcaatggt
      421 gaaggaagca ccatcacccc cacctccgct gagacccctt ccggcgagac ccctctccgg
      481 gagacggccg aagaattcgg gatagccttc ctgaaagact tcggcaaaca cgtcgggctc
      541 gatttccagg tcatggcgac ggacataagc gaaaaaacag acaggatact ctttcttgtc
      601 cagtgcgacg acggagatgt cctcatcggc aaggacgggg agatcctgga ggccctgcag
      661 catctcctga ggttggcggt agcaaaaaga ttcaaacata accttaaatt acttatagat
      721 atcaatggtt acagagaaag acgaaaaaaa gctttaacaa ttatggcaaa acgtcttgcc
      781 gataaagcaa aaaggtcagg aaagaagttc aagacggacc cattgaaccc ctacgagcgg
      841 cggattatcc acacactctt caagcacaac aagggcatca ccacgaagag cgaaggagag
      901 gggcacatca agagggtcgt cctcaccccg acgggaattc ctttcggcgg cgaccggggc
      961 ggtgcccggg gaggaccccg gggaggcgcc cggggaggtt tccgtggcaa cgcgcgcggg
     1021 agacgctgac accatatgtg ccgtttcgac acctcccggt gaaggcggca tcagcataat
     1081 acgcatcagc ggacttaagg cgcaccccat cctcgaaaag atcttcaggc cccgcggcag
     1141 gaaaccgcgc aaacgatcat acggcaggct tcgactggga cacatcgtgg accccgcgga
     1201 cgcgcgggag gtggatgagg tcctggccgt cctgatggcc gcccccgcca cctacacacg
     1261 cgaagatata gcggagatcc attcccatgg cggttttgcc gcgcagaagg ccgtcatgga
     1321 cctcgtcatg aggcagggcg cccgcctcgc cgagcccggt gaattcacgc gcagagcctt
     1381 cctgaacggc aggatcgacc tcgtgcaggc tgaatcggtc ctcgacctca tacgaagcga
     1441 aacggacgag gagctgcgat acgccctcac ctgtctcaag ggtggccttt cggcgaagat
     1501 gggtcttttc agagagacat tgaagcaggc cctcgtggcc gtggaggcaa cgatcgactt
     1561 ccccgatgaa gacctccgca tagatctctc aggacccttc gcgtccctca gaaaggcaaa
     1621 gaaggggatc gacgggcttg tatcctcata ctatgaagga aaagggctca agcagggctt
     1681 cgaagtcctt atactgggcc gcacgaacgt ggggaaatcg agcctcctca acgccctcgc
     1741 tctgagagag cgcgccatag tgaccgccct ccccggtacc acgcgcgacc tcatcgagga
     1801 aaccctctat ctgaaagggg cgaaggtccg tcttatcgac acggccggca tacgaacacc
     1861 cgagaacgtc gtggaagaag aaggcatacg ccgggtcagg gacaaggcgg cggaggcgga
     1921 cctcatcatc tgggtcctcg atggttcagc cccctattcc gacgacgatc agcgtgtttt
     1981 ggaagatatt cgtccatata ataaaatagt tgtagcgaat aaatgcgacc ttacacaata
     2041 tttggaatat aagctattgg tttcacaagg tataagtgat atactccata tttctgcaca
     2101 gaaagggatc ggtctcgatc acctcaggtc atcgctgttc tccgcgcttg cagggaagga
     2161 gcgaaagaat tctctcctca tcactaccct tcgtcacagg gatatcctca tgaaggtctc
     2221 cgccaacata accgccgcaa tcgatggtct cgaagcggga gctcctccgg agatcctcgc
     2281 gttcgagctc caagaagcgc tccaccacct cggggagatc acaggcgaaa cctgccccga
     2341 agagatcctc gacacaattt tcagccagtt ctgtataggg aagtgaaaaa cggcttgagt
     2401 cgcttgagtc gcttgaatcg cttgagcgtt ggaaagacct cccggaagta caacgccggg
     2461 aatagtatct agtctttttc ttttactctc aagccattca agcgactcaa gctattcaag
     2521 ccgttttctt attccttttg ttcgaaggcg ctgcaggcgc tagtttttga atgtcggtgg
     2581 ttttggaggc gcttctgacc ccaccggctt cgggggaacc acgcctgccg gagtgctcag
     2641 cgggaagcac acgttgtcct gcaggatccc aagaatgaca tcctgctcga agtacgtcca
     2701 cggaccattc tgcatcctgg cttccggctg ctccccggcc ctggcggagc cgaattcctt
     2761 ttcggcgatg acgcggtatc tgctggaacc acagtcgagt tccataaggg tctggacctt
     2821 ctccacctca ttgccgtctt tgtcgggttg tgttatccgc tcccacaccc tcacgacgcg
     2881 cttgtcgagt ctctcgatgc tgttggagtc gaaataatac ctcctcgtct cgtttcccat
     2941 gtagagcctc cactgcggtt cgcccttcgg cacctccttc ttcacctgtt ccccggatgc
     3001 tgtcgggggt tgaggctgag gctgcgccgt ctgcgccgca caggtgatag acactgccag
     3061 aacgatgaac actgcgatga ggcccatcaa aaatgctccc cttcctttca tggtcactcc
     3121 ttgtatgttc tcttgtgcca ctatcttccc atcccctcga tggtgttcgt attatactgc
     3181 gttccgccct cgcccgtcac gcccgagggc gccgtccagg agacctcggg gaaatcaccg
     3241 ccggggaaac gaccaaggac ggaccccatg aagctcatcc atatcggcaa acacacccgt
     3301 cccccgcttt ctcccttccc cagtgaggcc ctcgcgtcga acccgaccca tacgcctgtc
     3361 gcgacgtggg gagaataacc cacaaacaag gcatcatagt aattgctcgt cgttcccgtc
     3421 ttgcccgcca cgggataccc gatactggag gctcccttcg cggtcccata ctgcacggga
     3481 cccttcaaaa ggatattcat tgacgaggcc acttcctcgg gcagggccct ctccttcagc
     3541 gcggcattct cttcgagcac ggtgccgtcc tggtcgacta tcctcctgac gaatatgggc
     3601 tttatgcgat agcccccgtt cgcgaacgcg gcaaagccct tgacgagctc cagaagggtg
     3661 atattcgacg aacccagggc aatggagaga ttgtcctcga tgtcggcctc tatgccgatg
     3721 tccctgatcg tctccttgac tgggcctatg cctatctcct ccaatagcct gaccgtcgcc
     3781 gcgttcttcg agtaggcaat ggcatcccgc atcgatatgt cgccctggta cttgccgtca
     3841 taattccttg gtgtccagga cctccccatt cccgtggaat agctctttgg ttcatcatgg
     3901 agaacggaat cgggagtgta tcccttcttt agcgccgtca catagatgaa aggcttgaag
     3961 gcgctgccgg cctgcacttt tgccaccgtc gccctgttat aaggactctt ctcgaaatcc
     4021 cttcccccga cgatggcgta gacatacccc agattgacgt cgaggctcac aagggcgccc
     4081 tcgaccttca gcgagcgaac g
//
LOCUS       DAOPOA010000117         4098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_23674_length_4098_cov_3.380411, whole genome shotgun sequence.
ACCESSION   DAOPOA010000117 DAOPOA010000000
VERSION     DAOPOA010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23674_length_4098_cov_3.380411"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1948
                     /locus_tag="PKJ17_03595"
     CDS             <1..1948
                     /locus_tag="PKJ17_03595"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOD75085.1"
                     /translation="RSHYVPANETVVITGDFDSALGHRLITEWFSKDPSVAPPAERHV
                     PKDGSKGEKEKVLTRDLREKYIAVAYPIPRRTSTDVPALDILGVILGDGESSRLQEAL
                     KNKKGVVNGIETYVFTPKEEGLFVIYANHSANDRDSVLREIDREIGRIQKQGIKDWEL
                     VKARNMLRAYYVYDAETAQGRARQIGDAKTMTGDPHFIEKFLKDVDRVSADDVRKAAV
                     KYLRPDTRRIVVMGPAKAVKNPYRKTLGNGLTCLVNRNTSSPTFSFRIGFTGGVKAEQ
                     PGRNGEFNLLARMLLKGTKNKKSSEIARQIDLLAGTLVPYNGRNLFGLSGRFLSKDVK
                     EVLRLLKELLMETSMTERELTRVKREVLSEIRQRDDDPISFTFLRFNEAIFAGHPYSR
                     DPMGSEKDVEALSLTDLEGVYRKYVTPHGAVLALSGDIDEKEVFSWFEELFGRWEGPE
                     SPLARVEAGRAGKEVAVEKDIQQTHMVFGFVGPGLTDKDRYAAEVLDAVLSGMGGRIH
                     RVLREENPYAYAVTFFNQMAYETGAMGIYIGTSEKFAKDVIRIAREEIEKIRREGISE
                     KEVQDGKNYLIGNHYIRMQSNGAIVSSMCFDALYGLDPDLFKTWPGFIEKVTKEDVDR
                     VARRYLTLDSMVRLVVGKIKDNNK"
     gene            complement(2022..3155)
                     /locus_tag="PKJ17_03600"
     CDS             complement(2022..3155)
                     /locus_tag="PKJ17_03600"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD75086.1"
                     /translation="MNIPPINLKAQFKGVRKDIFKGIREILDEQKLILGQYCQELEGQ
                     VSSMTGGAHAISCANGTDALILSLMALGIGQGDEVVTTPYSFFSTASSIALVGARPVF
                     ADIQDSDMNIDPAKVEKAITPKTKAIIVVHLFGKICDMKRILGIAEKRGISVVEDMAQ
                     ALGAKRGGTAVGTFGDIAATSFYPTKNLGGIGEGGMVLTRRADLGEMARKLRVHGMGE
                     KPYIHEVIGFNSRLDEIKALALCVKLRKLASWNKTRLETAGYYNMHMRDLPLVLPTID
                     DETSHIFHHYVIRTVRRDALQLHLREKGIMTGIYYPLPLHLQPCFSYLGYKEGDLPIA
                     EEAARTSLAIPVFPELKKREKDYIIKGIRSFFEKTDEGSENGQ"
     gene            3502..>4098
                     /locus_tag="PKJ17_03605"
     CDS             3502..>4098
                     /locus_tag="PKJ17_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOD75087.1"
                     /translation="MKEAVIVSCARTAIGQFGQSLKDIPVVTLGGTAIREAIKRAGIR
                     PSKSKDKEFAPAVFEGKTDTELEAKFYDYDSGLKEVVIDEVIMGNVLQAGLGQNSARQ
                     ASIRGGVPKETAAYTINKVCSSGLRAIIAGAGSIMLGENEVVVAGGMENMSLAPFSLP
                     GLRFGARMFDTKAVDLMVLDGVWEIFYGYHMGFTAENIA"
BASE COUNT          921 a         1116 c         1214 g          847 t
ORIGIN      
        1 ccggtcccat tatgtgcctg ccaacgagac tgtcgtcata accggggact ttgacagtgc
       61 cctggggcac aggttgataa ccgaatggtt ctccaaggac ccatcggtcg cgccccctgc
      121 cgaaaggcat gttcctaagg acggatcgaa gggagagaaa gagaaggttc tgacaaggga
      181 cctgagggag aaatacattg ccgtcgccta tccgatcccc aggaggacaa gcaccgacgt
      241 cccggccctc gacattctcg gcgtcatcct gggagacggg gagagttcca ggctccagga
      301 ggccttgaag aataagaagg gtgtcgtcaa cggaatagag acatatgtct tcacccccaa
      361 ggaagagggg cttttcgtca tctacgccaa ccattcggcc aatgacaggg acagcgtgct
      421 ccgggagata gacagggaga tcgggaggat acagaagcag ggcatcaagg actgggaact
      481 cgtcaaggca aggaacatgc tgagggcgta ctacgtctat gatgccgaga cagctcaggg
      541 cagggcccgt caaataggcg acgccaagac catgacggga gatccccatt ttatagagaa
      601 gttcctcaag gatgtcgaca gggtatcggc ggatgacgtc cgaaaggcgg cggtgaaata
      661 tcttcggccc gataccaggc ggatcgttgt catggggccc gccaaggcgg tgaagaaccc
      721 gtaccgaaag acattgggaa atggcctgac atgcctcgtg aacaggaaca cctcgtcacc
      781 gaccttctcc ttcaggatcg gtttcaccgg cggtgtcaag gcggagcaac cgggaaggaa
      841 cggtgagttc aatctccttg cacggatgct cctgaaggga accaagaaca agaaatcctc
      901 ggagatagcc aggcagatag acctcctcgc gggcacactg gtgccctaca acgggcgaaa
      961 tctcttcggc ctgtcgggaa ggttcctgag caaggacgtg aaagaggtcc tccgactcct
     1021 gaaggaactc ctgatggaga cgtccatgac cgagcgggaa ctcacccggg tgaagagaga
     1081 ggtcctctct gagatacgcc agcgggatga cgatccgatc tccttcacct tcctgcgctt
     1141 caacgaggcg atattcgccg gccatcctta cagccgcgac cccatgggga gcgaaaagga
     1201 cgtcgaagcg ctgtccctca cggaccttga gggcgtgtac cgcaaatacg tcacgcccca
     1261 cggtgccgtc cttgccctat cgggagacat cgacgaaaaa gaggtctttt catggttcga
     1321 agagttgttc ggccgctggg agggaccaga aagtcccctt gccagggtgg aagccggacg
     1381 ggccggcaag gaggtcgccg tcgagaagga catccagcag acccacatgg tcttcggctt
     1441 tgtcggtcca gggctcacgg acaaggaccg gtacgcggca gaggtcctcg acgcggtgct
     1501 cagcggtatg ggcggcagga tacaccgggt cctcagggag gagaaccctt acgcctatgc
     1561 cgtcaccttc ttcaaccaga tggcctacga gacgggggcc atggggatct acataggaac
     1621 gagcgagaag ttcgcaaagg atgtgatccg tatcgcccgc gaggagatcg aaaagatccg
     1681 ccgggagggt atttccgaaa aagaggtcca ggacgggaag aactacctca tcggcaacca
     1741 ttacatcagg atgcagtcga acggcgccat cgtttcgtcc atgtgcttcg atgcgcttta
     1801 cggccttgac ccggaccttt tcaagacatg gccggggttc atcgaaaaag tgacgaagga
     1861 ggatgtcgac cgggtggccc gcagatacct cacccttgac tccatggtca gactggtggt
     1921 ggggaagata aaggacaata acaaatgacc ggggatggcc gggcctttcc catgacccat
     1981 cacctataac ccatatcctg cgttattacc ccttcttctg gtcattgacc attctcacta
     2041 ccttcatccg ttttctcaaa aaaggacctt attcctttga ttatatagtc cttttccctc
     2101 ttcttcaatt ccgggaaaac ggggatggcg aggcttgttc tggccgcctc ttcggcgatg
     2161 ggaagatcgc cttccttgta tcccagatac gaaaaacagg gctgcagatg aagggggaga
     2221 ggatagtata tgccggtcat gatccccttt tccctgaggt ggagttggag ggcgtcgcgc
     2281 cgcaccgttc ggatcacata atgatggaag atgtgtgatg tctcgtcgtc gatggtggga
     2341 aggacgaggg gaaggtccct catgtgcata ttgtagtaac cggcggtctc gagacgggtc
     2401 ttgttccagg aggccagttt ccttaatttg acgcagagcg ccagggcctt gatctcgtcg
     2461 aggcggctgt tgaacccgat cacctcatgg atatagggct tctcgcccat tccgtggaca
     2521 cggagtttgc gcgccatctc accgaggtcg gcacggcggg tgagcaccat gcccccctcg
     2581 ccgatacccc cgaggttctt cgtgggatag aaactggtcg ccgcaatatc gccaaaggtg
     2641 cccaccgccg tcccgccgcg cttcgccccc aaggcctgcg ccatatcctc caccaccgag
     2701 ataccacgtt tttccgcgat gccgaggatc cttttcatgt cgcagatctt cccgaagaga
     2761 tgcacgacga tgatcgcctt cgttttcggg gtgatcgcct tctcgacctt cgccgggtcg
     2821 atattcatgt cgctgtcctg gatgtcggca aaaacgggcc gcgcacccac gagcgcgatg
     2881 gaacttgccg tcgagaagaa gctgtatggc gtggtcacga cctcgtcccc ctgtccgatg
     2941 ccgagggcca tgagggaaag gatgagggca tcggtcccgt tggcgcacga gatcgcatgc
     3001 gccccgcctg tcatggagga gacctggcct tcgagctcct gacagtactg ccccaggatc
     3061 agtttctgct cgtccagtat ctcccgtatg cctttgaaga tgtccttcct gactcccttg
     3121 aactgcgcct tcaggttgat ggggggaatg ttcatattgc ggcatccttt cattatggga
     3181 tgtgatcggt tggggatcgg gttatgttag catattactt cccggcaact caatatttca
     3241 ttccctccac cacccggagc cgccgcgcgg taggcgttct cgcggacccg gcatcgtctt
     3301 tctggaatac gtcggcctgt ttagccccgg ctgcggtacg ctggtttggc ttctccctta
     3361 tgttacgagg gagccgccgc cggggcgtcg aaaaggacgg gaacgatgtg tcctcacaac
     3421 cattaaaacc gttgacaaat acttgcaatt gcgataaaaa cattgtgaaa tttttttcaa
     3481 ctgaactgaa aggagacctg tatgaaagag gcagttattg tttcgtgcgc cagaactgcg
     3541 atcggccagt tcggtcagtc actgaaggac atccccgttg taacgcttgg tggaacagcg
     3601 atcagggaag ccattaagag ggcggggatc aggccttcga agagcaaaga caaggagttt
     3661 gcccctgcgg tcttcgaggg gaagacggac accgaactag aagcaaagtt ctatgattac
     3721 gacagcggtc tgaaagaggt cgtgatcgat gaagtcatca tgggtaatgt tcttcaggcg
     3781 ggactcggtc agaactcggc gcgccaggcc agcatccggg gcggcgttcc gaaagaaaca
     3841 gctgcttata ctatcaataa ggtgtgctcg tcgggattga gggcgatcat cgccggcgct
     3901 ggctccatca tgctcggcga gaacgaggtt gtcgtagcgg gcggtatgga gaatatgagc
     3961 cttgccccct tttcactccc gggactgaga tttggagcac ggatgttcga caccaaggcc
     4021 gtcgacctca tggtcctcga cggcgtttgg gagatcttct acggttacca catgggcttt
     4081 accgcggaga acatcgcc
//
LOCUS       DAOPOA010000118         4089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_23740_length_4089_cov_3.787556, whole genome shotgun sequence.
ACCESSION   DAOPOA010000118 DAOPOA010000000
VERSION     DAOPOA010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23740_length_4089_cov_3.787556"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PKJ17_03610"
     CDS             complement(<1..232)
                     /locus_tag="PKJ17_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOD75088.1"
                     /translation="MEKMLRIGLCQMNSTVGDMTGNGERVVEWVEKAKAEEVDILALP
                     ELVITGYPPEDLLLKKGFVDANIRVITEIASRI"
     gene            complement(373..1602)
                     /locus_tag="PKJ17_03615"
     CDS             complement(373..1602)
                     /locus_tag="PKJ17_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HOD75089.1"
                     /translation="MASTVSQKLEAKEQELKILHEVAKDISDNLDLSELLHRIVGTVR
                     DFVKADSCLVYLYDEDNDELVLSASSDPKAESVGSISLKLGEGVTGWAAKEKQPVYLA
                     KEAYRDKRFKAFTSLKEDKYEALLSIPILAKNRIVGVMNLQNKKEHVYPEAQIKLLFT
                     IGRYLGSAIQNAITYDEVVKKAKQLDLLSEVSRTIVSDHYIKEILHLIVTMTAKVMDS
                     KICSVMLLDEKKEELVIAATQSLSNEYVNKPNLKVGQSISGKVVLEKRPLNVLDVTKE
                     PGYMFPDVARKEGFVSLLSVPMMIKDQVVGVINSYTTKEHVFTKEEIDILQAVANQAA
                     VAIENTNLSHEILAAKEALESRKLVERAKGILMRELGLNEDEAYRKIHKKSMDMRKTM
                     KEVAEAIILAFDIQKRT"
     gene            complement(1965..2636)
                     /locus_tag="PKJ17_03620"
     CDS             complement(1965..2636)
                     /locus_tag="PKJ17_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOD75090.1"
                     /translation="MKRYADNLKSVNLFADLKDTELETISRILYVNTYHRGQLIFQEG
                     EDGNALFVVLKGRVKVCLYDEEGREYVLDVIGKDGFFGELALIDELPRSANAIAMEGS
                     DLLIVRRADFTKLLMENPSISIHILKVLAGRLRVADERIKWLAFLNVEGRILKYLLEV
                     GARLGIKMKDYVIIERGPSQIEIANSCGCSRETVSRMISSLVRKGVISVRRRQYTLYP
                     GTRTF"
     gene            complement(2665..>4089)
                     /locus_tag="PKJ17_03625"
     CDS             complement(2665..>4089)
                     /locus_tag="PKJ17_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOD75091.1"
                     /translation="ITTDMALEDGLVLDREGFEKALSEQKERSKTGSKIKGEEWNEGH
                     LTILGRGLTGTFTGYGTLKDEGVIGAILVGNDTVNEISEGEEGELFLDTTPFYAESGG
                     QVDDSGTITLAGGGATARVTAVTKVKEDLFAHRVVVEKGSFHGGDRVSLAVDEEKRKG
                     TSRNHTATHLLQYALRRVLGDHVKQSGSLVEAGRMRFDFTHFEAMKEEQIRAVEDIVN
                     EKIMECVPVVVNVKSREDAIREGATALFEEKYGETVRVITIGDFSRELCGGTHVANTG
                     QIGSLYILGESSLASGVRRIEATTGRGALTYKRRMEGTLKAIAKRTNTEFDRVEDRVE
                     SLLGEIALKEKELDRFKQDIIAHRVDGAIAEAIDREGMKIVTLFVENASADDLRKVTD
                     VVRSKVKDCVVVVGTTGDEDKGLVVASVSKDLQKRYNAGKIIKALTAQYGGKGGGGPN
                     IAQGGIPGAKVPESLKKVEQFIDT"
BASE COUNT          738 a         1245 c         1137 g          969 t
ORIGIN      
        1 cgatacgcga cgcaatttcg gtgatgaccc tgatgttggc atcgacgaaa ccttttttca
       61 ggagcaggtc ctcgggggga tagcccgtta tcacgagttc cggcagcgcg aggatgtcca
      121 cctcctcagc ctttgccttc tccacccact ctacgaccct ttctccgttt cccgtcatgt
      181 cgcccaccgt tgaattcatc tggcataaac cgatccgcag catcttttcc atatatgcac
      241 cacccctcca aatgtaagaa gggcgtccaa agcagtttta agcgccctgg acgcccttgt
      301 ccaatcattc ttagcagctc tgctaaaaat ccaaataatc tgtcaatatg atagtgtgcg
      361 ggcacaccgt tgtcaagttc ttttctggat atcgaaggca aggatgatcg cctcagccac
      421 ttccttcatg gtctttcgca tatccatgct ctttttgtgg atcttccggt aggcctcgtc
      481 ctcgttgagt cccagctccc gcatcagtat gcccttggcg cgctcgacga gcttgcgcga
      541 ttccagagcc tccttggcgg caagtatctc gtggctgagg ttcgtgttct cgatggcaac
      601 ggccgcctgg ttcgccaccg cctgaagtat gtcgatctcc tccttcgtga agacatgctc
      661 cttcgtcgtg tagctgttga tgacgcccac gacctggtcc ttgatcatca tcggaacgga
      721 caggagggaa acgaagccct ccttcctcgc cacatcgggg aacatgtatc ccggttcctt
      781 cgtcacgtcg aggacgttga ggggtctctt ctccaggacc accttgccgc ttatggactg
      841 tccgaccttc aggttcggct tgttcacgta ctcgttgctc aggctctgtg tggcggcaat
      901 gacaagctcc tcctttttct cgtcgaggag catgacggaa cagatcttcg aatccatgac
      961 ctttgccgtc atggtcacga tgagatggag gatctccttg atatagtggt cggagacgat
     1021 cgtccgggag acctcggaga gaaggtcgag ctgctttgcc ttcttcacta cctcgtcgta
     1081 ggtgatggcg ttctgtatcg cgctgccgag gtacctgccg atggtgaaga ggagctttat
     1141 ctgggcctct ggatagacat gctccttctt gttctggagg ttcatgacac ccacgatcct
     1201 gttcttcgcg aggatgggta tggaaaggag cgcttcgtat ttgtcctcct tgagggaggt
     1261 gaaggccttg aaccgcttgt cgcggtatgc ctcctttgcc aggtagacgg gctgcttctc
     1321 cttggccgcc cagccggtga caccttcgcc cagcttcagg cttatcgacc ccaccgattc
     1381 cgccttcggg tcgctcgacg cggaaaggac gagttcgtca ttgtcttcgt catagaggta
     1441 gacaaggcag gaatcggcct tgacaaagtc cctgaccgta ccgacgatgc ggtgcaaaag
     1501 ctccgagagg tcgaggttgt cgctgatatc cttcgccacc tcgtggagga tcttcagctc
     1561 ctgctccttc gcctcaagtt tctgactgac cgtggaagcc attaatggat agcctttttt
     1621 tagatgttat cttagaggag aagacggccg ttgtcaataa aagagggaga taacaagaca
     1681 cgtggtcgaa gaatggtaaa cgatcccgtc tgtcctgctt cagatcgggg atgatgccga
     1741 ggcgacgagg aggggctccc ggaggcgtac gaccgtacgt cgaggacagt cccgacgaag
     1801 gcaacgaagg cagcgccccg gaggatggaa ggatcttctc tgtcctgctt cagatcgggg
     1861 atgatgccga ggcgacgagg aggggctccc ggaggcgtac gaccgtacgt cgaggacagt
     1921 cccgacgaag gcaacgaagg cagcgacccg atatgaagca ggacctagaa ggtgcgagtg
     1981 cccgggtata gcgtatactg ccttctcctc acgcttatca cgcccttcct caccagcgac
     2041 gagatcatgc gggagaccgt ttcccgggag cagccgcagg agttggctat ctctatctgt
     2101 gaaggccctc tctcgatgat gacatagtcc ttcatcttga tgccaaggcg cgccccgacc
     2161 tcaaggaggt acttgaggat gcggccttcg acgttcagga aggcgagcca cttgatcctt
     2221 tcatcggcaa cgcgcagcct tcccgcgagg accttcagta tgtggatcga aatggagggg
     2281 ttctccatga ggagcttcgt gaagtccgcc ctgcgcacga taagcaggtc cgacccctcc
     2341 atggcaatgg cgttcgccga acggggcaat tcgtcgatga gggcgagttc accgaagaaa
     2401 ccgtcctttc ctatgacatc gaggacgtat tcccgtccct cctcatcgta gaggcagacc
     2461 ttcacgcgcc ctttcaggac aacgaagagg gcattgccat cctctccctc ctggaagata
     2521 agctggcccc gatgataggt gttgacgtag aggatccggg agatcgtctc cagttctgtg
     2581 tccttgagat ccgcaaagag gttgacgctc ttaagattgt ccgcgtaccg cttcatctct
     2641 cccccgtcgt tttttcacat gatactaggt gtcgatgaat tgttcaacct ttttgaggga
     2701 ttccgggacc tttgcccccg gtatgccgcc ctgcgcgata ttgggtcccc cgccgccctt
     2761 tccgccgtac tgcgcggtaa gggccttgat gatcttcccc gcgttgtagc gcttctgcag
     2821 gtccttgctg acagaggcaa cgacgagccc cttgtcctcg tcccctgtcg tcccgacgac
     2881 gacaacgcag tccttcacct tggaccgcac gacatctgtc accttcctca ggtcgtcggc
     2941 ggaggcgttc tccacgaaga gggtgacgat cttcatcccc tctctgtcga tcgcttccgc
     3001 gatggcgccg tcgaccctgt gggcaatgat gtcctgcttg aagcggtcga gctccttttc
     3061 cttgagggcg atctccccga ggaggctctc gacacggtcc tcgacacggt cgaactcggt
     3121 gttcgtccgt ttcgcgatgg ccttcagggt cccctccatc ctgcgcttgt acgtaagggc
     3181 cccccggccc gttgtcgcct cgatcctgcg gacgcctgat gcgagagagc tctcaccgag
     3241 gatgtagagg ctgccgatct ggcccgtatt ggccacgtgc gtgccgccgc agagttcccg
     3301 ggaaaaatcg ccgatggtga tgacccgcac cgtctcgccg tatttctcct cgaacagcgc
     3361 cgtcgctccc tcccgtatgg cgtcctcacg gctcttcacg ttgacgacga cgggcacgca
     3421 ttccatgatc ttctcgttga cgatgtcctc caccgcccgt atctgctcct ccttcattgc
     3481 ctcgaagtgg gtgaagtcga agcgcatcct gccagcctcg acgagggagc ccgactgctt
     3541 gacgtggtcg ccgaggaccc ttctcaatgc atactggagg aggtgcgtcg ccgtgtgatt
     3601 gcgcgacgtg cccttgcgtt tctcttcatc gacggcgaga gataccctgt ctcccccgtg
     3661 gaacgacccc ttttcgacga cgacgcggtg ggcaaagagg tcttccttta ccttggtcac
     3721 cgccgtcacc cgcgccgtgg cgccgccgcc ggcaagggtg atcgtccccg agtcgtcgac
     3781 ctggccgccg ctttcggcgt agaagggcgt cgtgtcgaga aaaagctcgc cctcttcccc
     3841 ttcggatatc tcatttaccg tgtcgttgcc gacgaggatg gccccgatga cgccttcgtc
     3901 cttcagggtg ccgtacccgg tgaaggtgcc ggtgagaccc cggccgagta tggtgaggtg
     3961 gccctcgttc cattcctcgc ccttgatctt cgagcccgtc ttggagcgct ccttctgctc
     4021 cgagagggcc ttctcgaatc cctccctgtc gaggacgagg ccgtcttcca gggccatatc
     4081 cgtcgttat
//
LOCUS       DAOPOA010000119         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_23828_length_4077_cov_2.495276, whole genome shotgun sequence.
ACCESSION   DAOPOA010000119 DAOPOA010000000
VERSION     DAOPOA010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23828_length_4077_cov_2.495276"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKJ17_03630"
     CDS             <1..449
                     /locus_tag="PKJ17_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75092.1"
                     /translation="GEDVEKWGLAGLLHDIDIEITDADLSRHCLEAQKILTAEGIDPE
                     TVEAVVMHNEAVTGGRKRETRFQHALAAAETITGLIVATTLVYPDKKLASVKAKSVVK
                     RMKEKAFAASVNRDIILECEVIGIPLNEFAELSIAAMLGISGRLGL"
     gene            457..1743
                     /locus_tag="PKJ17_03635"
     CDS             457..1743
                     /locus_tag="PKJ17_03635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD75093.1"
                     /translation="MSHGGVKTGVLEKIRNSAYLQAFTNRRMAVMILLGFGSGLPLPL
                     TAGTLQAWLTVAGIDIKTIGIFSLVGVPYTLKFLWSPFMDRFVPPWLGRRRGWIVSMQ
                     FLLLLGIAAMPLTSPGSMPLVLAFIALGVAFFSASQDIVIDAYRTDVLPESERGIGVA
                     VFIFGYRIAMLVGGALALIMSDHIGWQKTYLFMALFMIFGMLGAFVGREPDHHIVPPR
                     TMQEAVWGPLKDFFSRKAAVVIILFIILYKLGDAYAGALSTAFLIRGVNFTPTDVGTI
                     NKGMGLVATIVGALFGGTLMVKMGLFRSLWYFGILQMVSNLSFMVLAIVGKSYPAIIV
                     AVAFENLSGGMGSTAFVAFVMALCNKRFSATQFALLSSLAVLGRVVISPTSGYIVTYI
                     GWANFFLFTTLAALPGLFLVLHLKGEITALDSRKTT"
     gene            1955..2155
                     /locus_tag="PKJ17_03640"
     CDS             1955..2155
                     /locus_tag="PKJ17_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOD75094.1"
                     /translation="MAKGTVKWFNESKGFGFITQDDGVDVFVHYSAIQDSGFKTLAEG
                     QEVNFDVVKGPKGLQAANVTKL"
     gene            complement(2249..2980)
                     /locus_tag="PKJ17_03645"
     CDS             complement(2249..2980)
                     /locus_tag="PKJ17_03645"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HOD75095.1"
                     /translation="MRTKIVLGKWSRKRIDEMLAEASKVDDVGERIAFLSRHFLDTPY
                     GESTLAGGPGTPEELVVNLAALDCFTFIDYVEAMRMTSSFEEFLEVLTRIRYRDGVVS
                     YAGRNHFLTDWKEHNGLFVRDVTGLIGRSRARKTLKTLNVRSDGSPFLEGIPARVRSV
                     EYIPSPMIDGDMLGELHTGDYAGIYTDIDGLDVSHVGIIIKEKRWVYLRHASSATASR
                     RVVDQLFGDYIREKPGVVVLRPLPE"
     gene            complement(3038..>4077)
                     /locus_tag="PKJ17_03650"
     CDS             complement(3038..>4077)
                     /locus_tag="PKJ17_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HOD75096.1"
                     /translation="AVVSIALLITVPFHQALAAGADRMAECAAIENDSARLKCYDTLS
                     GRAPGKSSVSPDTPAAKAPPVTGSANAEKIEDGSPPAEQSVLAKHWDLDERSRARRFV
                     IRPYRSNYVLPLAYNNSPNMDASLDLDPEARSQNAEAKFQISFKVKLWEDILGNDMDL
                     WFAYTQLAFWQIYNKQFSSPFRETNYEPELLLNFRTDYDLLGLKGRMINVGLNHQSNG
                     RSQPLSRSWNRVVATFGFERDRFNLLARTWYRIPENEKDDDNPDIDKFMGYGELWGFY
                     YWKKHRFAMMVRNNLRPSDNRGALQLEWSIPLIERVNGYLQYFNGYGESLIDYDKSVN
                     RFSIGFMLVDW"
BASE COUNT          814 a         1145 c         1158 g          960 t
ORIGIN      
        1 tcggtgaaga cgtcgagaaa tggggccttg caggtcttct gcacgatatc gacatcgaga
       61 tcaccgacgc cgatctctcc cggcactgcc tcgaggcgca aaagatactg accgccgaag
      121 gcatcgatcc tgaaacggtt gaagccgttg tgatgcacaa cgaggcggta acggggggca
      181 ggaagcgcga gacccgcttt cagcacgccc ttgccgcggc cgagacgatt acgggtctca
      241 tcgttgccac gaccctcgtg tatcccgaca agaagcttgc cagcgtgaag gcaaagtccg
      301 tggtcaagcg catgaaggaa aaggccttcg cggcttccgt gaaccgcgat atcatcctgg
      361 aatgcgaggt catcggcatt ccccttaacg agttcgccga actgtccatc gccgcgatgc
      421 tcgggatcag cggccggctg ggattgtgag agcgcggtga gccatggcgg cgtaaaaacg
      481 ggtgtgctgg agaagatcag gaattctgcc tacctgcagg cgttcacgaa ccgccgcatg
      541 gcggtgatga tccttctcgg attcggttcg ggcctgccct tgcccctcac ggcgggaact
      601 ctccaggcat ggctgaccgt ggcgggcatc gacatcaaga ccataggcat cttctcgctc
      661 gtgggggttc cgtacacgct caagttcctc tggtcaccct tcatggacag gttcgttccg
      721 ccgtggctgg gcaggcggcg tgggtggata gtctccatgc agttccttct gctcctcggc
      781 atcgccgcga tgcccctgac ctctccgggc agcatgcccc ttgtcctcgc cttcatcgcg
      841 ctgggggttg ctttcttctc ggcctcccag gacatagtca tagacgccta tcgcacggac
      901 gtcctgcccg agagtgagcg gggcatcggt gttgccgtct tcattttcgg atacaggata
      961 gccatgcttg tcggaggggc gctggccctc atcatgtcgg accatatcgg ttggcagaag
     1021 acgtatctct tcatggccct tttcatgatc ttcgggatgc tgggagcctt cgtcggacgc
     1081 gagcccgacc accacatcgt accgccgcgg acgatgcagg aagccgtctg gggacccctc
     1141 aaggatttct tctcgcgcaa ggcggccgtc gtgatcatcc ttttcattat tctgtacaaa
     1201 ctcggcgatg cttacgcggg cgcgctttca acggccttcc tcatacgggg cgtgaacttc
     1261 actcccaccg acgtggggac gataaacaag gggatgggtc ttgtggccac catcgtgggg
     1321 gccctcttcg gagggaccct catggtgaag atggggcttt tcagatcgtt gtggtacttc
     1381 gggatcctcc agatggtgtc gaacctatcc ttcatggttc tcgccatcgt cggaaagagc
     1441 tatcccgcga taatcgttgc cgtcgccttt gagaaccttt ccgggggcat gggatcgacg
     1501 gccttcgtcg ccttcgtcat ggccctgtgc aacaagcgtt tcagcgccac ccagtttgcc
     1561 ctgctgtcgt ctctcgcggt gctgggacgc gttgtcatat ctccgacgtc gggatacata
     1621 gtgacctata tagggtgggc aaacttcttt ctttttacta ctctggcagc cttgccgggc
     1681 cttttcctcg tcctgcatct gaaaggagag atcacggccc tcgattcgcg aaagaccacc
     1741 tgatcggaag acccccggta ggcgcggttc ctcggggatc ctttccatta aaatttgttc
     1801 ataaatttca ttgacagctt tttgatactg tgggatactg acaacgaagt ttcggaagtg
     1861 gctgtcaata gtgatgaaac gcgttttttg cctgaccatc cccgtgacac agctccgtaa
     1921 tcttcaaaaa aacacatagg agggctttag taagatggca aaaggaactg tgaagtggtt
     1981 caatgagtcc aaaggttttg gtttcattac gcaggatgac ggtgtggatg tttttgtcca
     2041 ctactcggca atccaggact ccggtttcaa gacccttgcg gaaggccagg aagtcaactt
     2101 cgacgttgtg aaaggtccga aaggactgca ggcggccaac gtcaccaaac tataaaagac
     2161 aaaaaaaggg ggtccgatcg tatccaggcc gggcccttta taatatcttc aaggcagcat
     2221 tttcctgcgg taagctctta aggggggttt attccgggag gggtcttaat acaacaacgc
     2281 ccggtttttc tctgatgtag tccccgaaca attgatcgac caccctgcgg gaggccgtcg
     2341 ccgacgaggc gtgacgcagg tatacccatc ttttttcttt aatgattata cccacatgag
     2401 atacgtcgag cccatcgata tccgtataga tgccggcata gtcgccagtg tgcaattcgc
     2461 ccagcatatc cccgtcgatc atgggggagg ggatgtactc cacggacctt acccgggccg
     2521 ggatcccttc caggaatgga cttccgtcgg acctcacgtt gagggtcttc agggttttac
     2581 gcgcccggct cctgcctatg aggcccgtga catcgcggac gaagagcccg ttgtgttcct
     2641 tccagtcagt gaggaaatgg ttgcgcccgg cataggagac aaccccgtcc ctgtaccgga
     2701 tccttgtcag gacctcaagg aattcctcaa aggaagatgt catgcgcatc gcctcgacgt
     2761 agtctatgaa cgtgaaacaa tcaagggcgg cgaggttgac gacaagctct tccggtgtcc
     2821 cgggacctcc cgccagggtg gattctccgt agggtgtgtc gaggaagtgt cgtgagagaa
     2881 aagctatccg ctcgcccacg tcgtcgacct tcgaggcctc ggccagcatt tcgtcaatgc
     2941 gtttccgtga ccattttccc agcactatct ttgttctcat gcctgtaaac tcaccatgtc
     3001 ttcccttgaa cacctgaagg ccggacgtct ttggccccta ccagtcgacg agcatgaaac
     3061 ctatgctgaa acggttgacg ctcttgtcgt aatcgatgag gctctccccg tagccgttga
     3121 aatactgcag atagccgttg acacgctcga tgaggggaat gctccactcc agttgcaacg
     3181 cgcccctgtt atcggaaggc ctcaggttgt tgcgcaccat catggcgaac ctgtgtttct
     3241 tccagtaata gaaaccccac agttccccat accccatgaa cttgtcgatg tcggggttgt
     3301 cgtcgtcctt ttcattctcg gggatcctgt accatgtcct ggcgagaagg ttgaacctgt
     3361 cccgctcgaa accgaaagtc gctaccaccc ggttccaact gcgcgacaat ggttgggatc
     3421 tgccgttcga ctggtggttg agcccaacgt tgatcatcct tcctttcaga cccagaaggt
     3481 cgtaatcggt gcggaagttg agaagcagtt cgggttcgta attcgtttcg cgaaaagggg
     3541 atgagaactg cttgttgtaa atctgccaga atgcaagctg ggtgtaggcg aaccacaggt
     3601 ccatatcatt cccgagaatg tcttcccaga gcttcacctt gaaactgatc tggaacttgg
     3661 cctcggcgtt ttgcgacctt gcctcggggt cgagatcgag gcttgcgtcc atgttgggag
     3721 aattgttgta cgcgaggggg aggacatagt tggagcgata gggcctgatg acgaatcgtc
     3781 ttgcccggct tcgctcatcg aggtcccagt gtttcgccag aaccgactgc tcagccggcg
     3841 gtgatccatc ttcgatcttc tccgcattcg cagatcccgt caccggtggg gctttggctg
     3901 caggagtatc gggtgatacg gacgatttcc ccggtgcccg gcccgataac gtgtcgtagc
     3961 acttcaaccg ggcgctgtcg ttctctatgg cggcgcattc ggccatcctg tcggcgccgg
     4021 cggcgagggc ctggtggaac gggacggtaa tcagcagggc gatggacact accgcta
//
LOCUS       DAOPOA010000120         4068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_23887_length_4068_cov_3.750311, whole genome shotgun sequence.
ACCESSION   DAOPOA010000120 DAOPOA010000000
VERSION     DAOPOA010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23887_length_4068_cov_3.750311"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2147
                     /locus_tag="PKJ17_03655"
     CDS             <1..2147
                     /locus_tag="PKJ17_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOD75097.1"
                     /translation="MKGPFVLARFGERGAGRMEEIKAERGSNRSTRSTKTAFLPLWNG
                     DLVFANREPAIGIVRSNGQTLFYKDATALWFVGDDGLLASTDGTEVRFNVPGKEKRTI
                     RFSLRDRRLDEIRGNDQGEMKLFKTDPGVPAIRLTDWRSSESPKLNNRPIKLRRGEHS
                     VARAVAADGRSFALGTNLAIYLFGADGSEIWRQPFPGVRGVSIPPGGRTVIAALSDGT
                     IHWHGIDDGRVLLTLFPSVDMKRWVVWTHSGYYDAAPGAEELVGWHVNRNGRLAADFF
                     PVSRFRSIYYRPDVVAKTLEMGDEKKALAAADRGSGRATQDIALKKVLPPVVSVIHPA
                     DGAEVREREIDVRFTVRSPSGDPVTGIRVLVDGRPVQRIRGVAAEGEETRDVTITIPE
                     KDSEISVLASNKNAVSEPSTVRVRWSGPAAGTKDASSLQAQYEKAQKNVTQKAGNPPG
                     PASRDASAATPGEFVVKPKLYILAVGVSSYRDKELKLGFAAKDAEDFAGIMSRQRGEL
                     YRDIVVRRLIDEKATKDEILDGLDWITKETTSKDVAMVFLAGHGINDPNGIYYFLPVN
                     TDLDRLKRTGLAFSDIKNTVISLAGKTVLFVDTCHSGNIMGSRRAVADINALVNELAS
                     AENGAVVFSSSSGRQFSVEDASWGNGAFTKALVEGIGGKADFLGKGRITINMLDLYLS
                     ERVKELTKGRQTPTTAKPTTVPDFPIAVLEKR"
     gene            complement(2365..>4068)
                     /locus_tag="PKJ17_03660"
     CDS             complement(2365..>4068)
                     /locus_tag="PKJ17_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75098.1"
                     /translation="GMSVYFQDITEHRQMEQRFEAHRLLLEGIMDQMPAGVSIMKGND
                     LRFIYVNPAYQSFARDRKMVGRTLREVWPEVYRRLERIFRDVLETGQRHAEVDQLYLA
                     RRSPGDPVEERYFTWSLARITMPDGDPGLLNTAWDTTETKKLGEEVRRSRDELELRVR
                     ERTAELEKAYDLLTKETAEREQAEQQLRRGQKLEALGTLAGGIAHDFNNVLTGVIGFA
                     EMVLERLTPGSKEHRRLELALKGAYRGRDLVKQILAFSRKGEQERKPLALARTVDEAL
                     ALLRPALPSTIETVWKSPMEGCRVLADPVQMQQVIMNLCTNAAHAMREEGGVLEIAIS
                     EVLVTPEGPPPFPEMEPGEYVVLEVGDTGCGMSPDTLEQIFDPFFTTKKEGEGTGLGL
                     SVTYGIVRSHGGHIAVESEPGKGTRFDVYLPKLTEAGAIEDVRAPSSMGGNERILLVD
                     DEDLLVELNGERLKDLGYDVVSTTASIEALRIFQEEPDRFDLVLLDQTMPNLTGVDLA
                     AKLLARRPDIPIILCTGRADSVSLEALRQAGIRDLLLKPFNKQELAGAVRRVLDAKIS
                     A"
BASE COUNT          795 a         1288 c         1232 g          753 t
ORIGIN      
        1 gcatgaaggg gcccttcgtc ctggcgcggt tcggtgaaag aggcgcgggg cggatggagg
       61 agataaaggc cgagcgaggc tccaaccgct cgacgcgaag cacaaagacc gccttcttgc
      121 ccctctggaa cggcgacctc gtcttcgcca acagggagcc cgccataggc atcgtgagga
      181 gcaacggtca gacccttttc tacaaggacg cgacggcgct ctggttcgtg ggtgatgacg
      241 gtctactcgc ctcgaccgac ggtactgagg tccggttcaa tgtccccggc aaggagaaga
      301 gaacgatccg tttctccctt cgtgacaggc gccttgacga gatacgtggg aacgatcagg
      361 gcgaaatgaa gcttttcaaa accgatcccg gcgtaccagc catccgcctc acggactggc
      421 ggtcttcgga gtcgcccaag ctgaacaaca ggccaataaa gcttcgccgc ggggaacact
      481 ccgtcgcgag agcggtggcc gcggacggca ggagctttgc cctgggcacg aacttagcca
      541 tatatctttt cggcgccgat ggctccgaga tctggaggca gccctttccc ggagtacgcg
      601 gcgtcagcat tcctccgggc ggacgcaccg tcatcgccgc gttatcggac ggcaccatcc
      661 actggcacgg catcgatgac ggaagggtcc ttctcacgct ctttccctcc gtcgacatga
      721 aacgctgggt cgtctggaca cattcaggct actatgatgc cgcgccgggc gccgaagaac
      781 tggtgggctg gcatgtcaac cgcaacggca ggctcgccgc cgatttcttc cccgtctccc
      841 gtttcaggtc catttactac cggcccgacg tcgtggcaaa gaccctggag atgggcgatg
      901 aaaagaaggc cctcgcggcg gccgacaggg ggtcgggccg cgccacacag gacatcgccc
      961 tcaagaaggt cctgcccccc gtggtatccg tcatccaccc cgccgacggg gccgaggtca
     1021 gggaaaggga gatagacgtc cggttcaccg tgcggtcacc ttcgggagac cccgtcaccg
     1081 gcatcagggt gctcgtcgac ggcaggcccg ttcagcgcat ccgcggggtg gcggcagagg
     1141 gcgaggagac acgcgacgtg acgatcacga taccggaaaa ggactcggag atctccgtcc
     1201 tggcctccaa caagaatgcc gtgagcgaac cctccacggt gcgcgtcagg tggtccggcc
     1261 ctgccgcggg gacaaaggat gcctcgtccc tgcaggcgca gtacgaaaag gcccaaaaaa
     1321 atgtgacgca aaaggccggg aaccccccgg gaccggcttc ccgggacgcc tccgccgcga
     1381 cgcccggtga attcgtcgtc aagcctaagc tttacatcct cgctgtcggc gtctccagct
     1441 accgggacaa ggagctcaag ctcggtttcg ccgcgaagga tgccgaggac ttcgccggga
     1501 tcatgtcgcg ccagaggggc gagctctacc gggacatcgt cgtcaggagg ctcatcgacg
     1561 agaaggcgac gaaggacgag atcctcgacg gcctcgactg gatcacgaag gaaacgacaa
     1621 gcaaggacgt ggcgatggtg ttcctcgccg gtcacggcat caatgacccc aacggcatct
     1681 actacttcct gcccgtgaac acggacctcg acaggctcaa acggacgggc ctcgccttct
     1741 cggacataaa gaacaccgtc atctcgctgg cgggcaaaac ggtcctcttc gtcgacacgt
     1801 gccactcggg caacatcatg ggctcgcggc gcgccgtggc ggacatcaac gccctcgtca
     1861 acgaactggc aagcgcagag aacggggccg tcgtcttcag ctcctcctcc gggcgccaat
     1921 tctccgtgga agacgcctcc tggggcaacg gcgctttcac aaaggccctc gtcgaaggca
     1981 taggcggaaa agcggacttc ctgggcaaag gccgcatcac catcaacatg ctcgacctct
     2041 acctgtcgga gcgggtgaaa gagctgacga aaggcaggca aacacccacg acggcgaaac
     2101 ccaccacggt gccggacttt cccatagcgg tgctggagaa aagatagtcg cagccggttg
     2161 aatcgcttga caggctgttg acaaagtgat ttctgaagac ggttcgtgct ctttcgtcat
     2221 tccagcgaag gacggaatcc aggaaaaacg aggggttaga aaaacctatg gatttaaggc
     2281 cttcgccgga atgacataga aaatgggttt atcaacagcc tgttgagggt tgaagacggg
     2341 attcgaaacc ggagccaggg tggctcaggc gcttatcttt gcatcgagga cgcggcggac
     2401 cgctcccgcc agttcctgct tgttgaaggg cttcagcagg aggtcacgga tgcccgcttg
     2461 ccgcagcgcc tctaacgaga cactgtcggc ccgacccgtg cagagaatga tcgggatgtc
     2521 gggccgcctc gcgaggagtt tcgccgccag gtccaccccc gtcaggttcg gcatggtctg
     2581 gtccaggagg acgaggtcaa agcggtccgg ctcttcctga aagatccgca gtgcctcaat
     2641 gctggccgtg gtcgagacga catcataacc gaggtctttc aatctctctc cgttcagctc
     2701 gacgaggagg tcctcatcgt ccacgaggag aatgcgctcg ttcccgccca tggaggatgg
     2761 agcacgtaca tcttcgatgg ctccggcctc cgtgagcttc ggcagataga catcgaaccg
     2821 cgtccctttt cccggctcgc tctccaccgc gatgtggcct ccatggcttc tcacgatgcc
     2881 ataggtgacg gacagaccga ggcccgttcc ctctccttcc ttcttcgtgg tgaaaaaggg
     2941 atcgaatatc tgttccaggg tgtcggggct cattccgcag ccggtgtcgc ccacctccag
     3001 gaccacgtat tcccccggct ccatctcggg gaaagggggc ggaccctcgg gcgtcacaag
     3061 cacttccgag atggctatct ccaggacgcc cccttcctcc cgcatcgcgt gggcagcgtt
     3121 ggtgcagagg ttcatgataa cctgctgcat ctgcacgggg tcggcaagca cccggcaacc
     3181 ttccatggga ctcttccata cggtctcgat cgtggacgga agcgcgggcc tcagcagggc
     3241 aagcgcctca tcaacggtcc gggccagcgc cagcggtttc ctttcctgct cgcccttgcg
     3301 gctgaaagcg aggatctgct ttaccaggtc gcggcccctg taggcccctt tgagcgccag
     3361 ctccagcctt ctgtgctcct tgcttccggg cgtcagcctt tcgagcacca tctccgcgaa
     3421 acctatgaca ccggtgagga cattgttgaa atcgtgggca atgccgcccg ccagggtccc
     3481 cagggcttcc agtttctgcc cccggcggag ctgctgctcc gcctgttcgc gttcggcggt
     3541 ctccttcgtg agcagatcat aggctttctc cagctcggcg gtgcgctccc tgacgcgcag
     3601 ctcgagttcg tcgcgggacc tgcgcacctc ctcaccgagt ttcttggtct ctgtggtatc
     3661 ccatgcggta ttgagcaaac ccgggtctcc gtcaggcatg gtgatccggg ccagggacca
     3721 cgtaaaatag cgttcctcga cggggtcgcc gggagaacgt ctcgcgaggt aaagctggtc
     3781 gacctcagca tgacgttgcc cggtctcgag gacgtcgcgg aatatccttt ccagacgccg
     3841 atagacttcc ggccacacct cccgcagggt tcttcccacc atcttcctgt ctcgtgcaaa
     3901 ggactggtag gcagggttga catatatgaa ccgcaggtcg ttccccttca tgatgctgac
     3961 cccggcgggc atctggtcca tgatgccttc gaggaggaga cggtgggcct cgaaccgctg
     4021 ctccatctgt ctgtgctccg tgatgtcctg gaaatagacg gacatccc
//
LOCUS       DAOPOA010000121         4040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_24113_length_4040_cov_3.599498, whole genome shotgun sequence.
ACCESSION   DAOPOA010000121 DAOPOA010000000
VERSION     DAOPOA010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24113_length_4040_cov_3.599498"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="PKJ17_03665"
     CDS             <1..1882
                     /locus_tag="PKJ17_03665"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOD75099.1"
                     /translation="TTTEGPVLVIAGAGSGKTRVIEYRSLHLIQKDIEPESILLLTFT
                     RRSAQEMIERASRNDPRCSRIDGGTFHSFASKVLRVYSRNLGLSNSFTIYDEGDAEEA
                     IRRCCNKMGYDRDKEFPKKNELKNVFSLCVNKGMDVEDAVLRKYVDYEDRLGEIRHVQ
                     KEYAEYKLRNNCVDYDDLLVYLRVVLGIDEVRERLSSRYRYIMVDEFQDTNGIQADIV
                     RLLAKRHNNVMVVGDDAQSIYGFRGANHTNILRFPGEFEGCRVIMLEQNYRSTQSILD
                     VGNAVLNTMTRKFEKRLVAANGEVGEKPVVLRFEDIYEEAAWVGRKVQNLRAKGIALR
                     DIAVLFRTAFTSMPLQSELVRQGIRFKLFGGRKFYEMANVRDVVSYLRLIANPADELA
                     WHRVLLLLPGIGARTVERLLEMITHYTDPKDIIERVFAPLCTGQKNSDVFASFARLLQ
                     TLLTPSMTPSRQVDAVMEHYGPIMESKYKKDPLKVRELAMLGQMARRYTNLKDFLADV
                     SVDPDKDTNDDDVEYLTLSTVHSAKGLEWGRVFLIGLVDGVFPSSRSFDEGDGGDIEE
                     EKRLLYVAVTRAKEELFMSYYTKKGVNERSKAALCRFLDRANVQKTFEARTVKPSTRT
                     SR"
     gene            complement(1933..3123)
                     /locus_tag="PKJ17_03670"
     CDS             complement(1933..3123)
                     /locus_tag="PKJ17_03670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HOD75100.1"
                     /translation="MKKTLQELFGALGRNSPDIPFAVRFWDGQTETYGNGPTAFTITF
                     KTGTAARRCLGNGTLGFREEYVAGNIDVDGSLQQLVRLGVDPRFRNAKLSPGARLAIL
                     VQHIRSLNTTTRSPGNITHHYDLGDEFYRYYLDESMTYSCAYFRNEEDTLEQAQQQKY
                     EHICRKLQLKEGETLIDIGCGWGGMLLYAARHYGVKGLGCTLSPHQAEYARKRAAAKG
                     LEKDITISLEDYRDVKGRFDKFVSIGMFEHVGKGFIPQFMDEAKELLKPGGIGLIHTI
                     GKELDTAEDSWTMKYIFPGGYLPMLDHVIRTMGERGLSPVDIENLRLHYARTLDEWSK
                     RFEANAREIREMFDDGFVRMWRMYLNGSSAAFRWGDTRLYQILFTNGLNNSLPMTREH
                     LYRT"
     gene            3313..3654
                     /locus_tag="PKJ17_03675"
     CDS             3313..3654
                     /locus_tag="PKJ17_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75101.1"
                     /translation="MDENKRRGCATQRDLTVMMMEIKLEPSLTHCIETTAKREYERVL
                     GQLLKGKEEDSQLAEELELLRIFLESADFGQLRSRCDDSLTAGKGVEARLTSSSSAPG
                     YEIEIVEIDGA"
BASE COUNT          904 a         1043 c         1204 g          889 t
ORIGIN      
        1 taccaccacg gaggggcctg ttcttgtcat cgccggtgcg gggagcggga agaccagggt
       61 catagaatac cgctcgcttc accttataca aaaggatatt gagccggagt cgatcctcct
      121 tttgacgttt acccgcagaa gcgctcagga gatgatcgaa cgggcttccc ggaacgatcc
      181 gagatgttcg aggatagacg gcggcacctt tcactcgttt gcgagcaagg tccttcgggt
      241 gtacagcagg aatcttggcc tgtcgaattc cttcaccata tatgacgaag gtgacgcgga
      301 ggaggcgatc cggcgttgct gcaacaaaat gggctacgat cgcgacaagg agtttcccaa
      361 gaagaatgaa ctgaagaatg tcttcagcct ctgtgtcaat aagggaatgg acgtcgaaga
      421 cgcggtcctg aggaagtacg ttgactacga ggatcgcctc ggcgagatca ggcatgttca
      481 gaaggaatat gccgagtata agctcaggaa caactgtgtc gattatgatg acctcctggt
      541 ctatctcagg gttgtcctcg ggatcgacga ggtcagggag agactctcat cgcgataccg
      601 gtacatcatg gtcgatgaat tccaggacac gaacggcatc caggcggata tcgtccgtct
      661 tctcgcgaaa agacacaaca atgtgatggt cgtgggtgac gacgcgcaga gcatctatgg
      721 gttccgggga gccaaccaca ccaacattct gcgatttccc ggggagttcg aaggctgcag
      781 ggtgatcatg ctcgagcaga attaccgaag cacccagtct attctggatg tgggcaacgc
      841 cgtcctcaac accatgacgc gtaaattcga gaagcgcctg gtcgcggcga acggcgaggt
      901 tggcgaaaaa ccggtggtgc tgcgatttga ggatatctac gaggaggcgg cctgggtggg
      961 gcgcaaagtg cagaacctca gggcgaaggg catcgctttg agagatatcg ccgtgctgtt
     1021 cagaacggca ttcacctcga tgccccttca gtcggaattg gtaaggcagg gcatacgctt
     1081 caagctcttc ggcggccgca agttctatga aatggccaac gtgcgggacg tcgtctcgta
     1141 cctccggttg atcgcgaacc ccgccgacga gctggcctgg caccgcgtcc tgctcctttt
     1201 gccgggtatc ggcgcgagga ccgtggaaag gctcctcgag atgatcaccc actacaccga
     1261 tccgaaggac atcattgaaa gggttttcgc cccgctatgc acgggccaga agaactcgga
     1321 cgtcttcgcc tcgttcgcga gacttctcca gacgctcctt accccatcca tgacgccgtc
     1381 gcggcaggtg gacgccgtca tggaacacta tggtcccata atggagagca aatacaaaaa
     1441 agaccccctc aaggtcaggg agttggccat gttggggcag atggcccgaa ggtatacgaa
     1501 cttgaaggat tttcttgccg atgtgtccgt tgatcccgac aaggatacga atgacgatga
     1561 cgtcgaatac ctcaccctct cgacggtgca ttcggcaaag ggtctcgaat ggggcagggt
     1621 ctttcttatc ggcctcgtgg acggcgtttt cccttcgagc aggtcattcg atgaaggcga
     1681 cggtggggat atcgaggagg agaaacggct tttgtacgtg gcagttacaa gggcgaaaga
     1741 agagctcttc atgagctact acaccaaaaa aggcgtgaat gaacggtcca aggcagcgct
     1801 gtgccggttc ctcgatcgtg ccaatgttca aaaaacgttt gaggccagga cggtaaagcc
     1861 gtcaaccagg acatcccgct gatcacatta cgtttcccca gggcctcaaa gtctgtgccc
     1921 tgaagctccg atctaggtcc ggtagagatg ctcccgcgtc attggcaggg agttgttcag
     1981 tccgttggtg aagaggatct gatagagcct tgtgtctccc cagcggaagg ctgccgagct
     2041 gccgttgagg tacatgcgcc acatgcggac aaaaccgtcg tcgaacatct cccgtatctc
     2101 ccgcgcgttc gcttcgaacc gcttgctcca ttcgtccagg gttctcgcgt agtggaggcg
     2161 aaggttttcg atatcgaccg gggagaggcc ccgctcgccc atggtgcgga taacatgatc
     2221 aagcatgggg aggtaacccc cggggaagat gtatttcatg gtccaagaat cctcggcggt
     2281 atcaagctcc ttgccgatgg tgtggatcag gccgatgccg ccgggtttca gcaattcctt
     2341 tgcctcgtcc atgaattgag ggatgaatcc ttttcccacg tgctcgaaca tgccgatgga
     2401 gacgaactta tcgaagcggc ctttcacgtc gcgatagtct tcgagggaaa tggtgatgtc
     2461 cttttcgagt cctttcgctg ccgccctttt ccttgcgtac tcagcctgat gaggggacag
     2521 ggtacacccc aggcccttga cgccgtaatg gcgcgcggca tagagaagca tgccgcccca
     2581 gccgcacccg atatctatca gcgtttcccc ttccttcagt tgaagtttcc tgcagatgtg
     2641 ttcgtacttt tgctgctgcg cctgttccag ggtgtcctct tcgttcctga aataggcaca
     2701 agaataggtc atgctctcgt caaggtaata ccggtagaac tcgtctccca ggtcgtagtg
     2761 atgagtaatg ttcccgggtg accttgttgt ggtgttgagt gatctgatgt gctgcacgag
     2821 aatggccaat ctcgcgccgg gtgagagttt cgcattccgg aaacgggggt caacgccgag
     2881 acgaacgagc tgctgcaggc tgccatcgac atcgatgttg ccggcgacgt attcttccct
     2941 gaacccgagc gtgccgttgc caagacagcg tctggccgcc gttccggtct tgaacgtgat
     3001 agtgaaggcc gtcgggccat tcccgtaggt ttcggtttga ccatcccaga agcgcactgc
     3061 aaagggaata tcgggagaat tacgacccag cgctccgaac aattcttgaa gcgttttttt
     3121 cataggttcc tccagggtga gccgccgccc caggtgcggt tactaaagtc tatcaaattc
     3181 gggaggggga ggacagaaac atactctctt cgcgtcattc tgcggtgcgt cgagccgggc
     3241 agcacatcga ctgcttcttc aggcagcgag cggacgctct tgcaatgttg acatctttgc
     3301 ggtatcaagt gtttggatga aaacaagcgc aggggttgcg cgacacagag agacctgacg
     3361 gtgatgatga tggagatcaa gctggaaccg agtcttacgc attgcatcga aacgacggca
     3421 aagagggaat atgagagggt gctgggtcag cttctcaagg gaaaggaaga agatagccag
     3481 ctggccgagg aactggaact attgaggatc ttcctggaat cggcagactt cgggcagttg
     3541 aggagccgtt gtgatgattc cctgacagca ggcaaagggg tcgaggccag actgacgtcc
     3601 tcaagctccg caccgggata tgagatcgag atcgttgaga tcgacggagc gtagcacatg
     3661 gcctggatag cgggtattcc acaatgaggg acagggagac cttatgaaaa gtgatgtcag
     3721 cctccgccgc atcgatcccc gggaggtgcc tgtcgttggc acgctgatcc gggataccat
     3781 gagggcgtcc tatccggtct tctatccccc gcggggtact gggagggcgt caaggcgctc
     3841 ggtgagaagc acgacgatta ggtcctatcc tgttgatatc tcttcctttt attgaggttt
     3901 tgtggcatca taatggcagg agatcaagac gttcccgtga ctctgggggc gaaaggagag
     3961 aaggggttgt tttcgatatt tcactcgccg tcaaaagaca ttggcattga cctcggaacc
     4021 gcgaatacgc tgttctacct
//
LOCUS       DAOPOA010000122         4024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_24230_length_4024_cov_2.831192, whole genome shotgun sequence.
ACCESSION   DAOPOA010000122 DAOPOA010000000
VERSION     DAOPOA010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24230_length_4024_cov_2.831192"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1649)
                     /locus_tag="PKJ17_03680"
     CDS             complement(138..1649)
                     /locus_tag="PKJ17_03680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD75102.1"
                     /translation="MESTMLEGSNLTVPNDLGYLPAIQAFVSEVMERRGYSKRDTTMF
                     LIALEEAIVNVVKHAFEPGEKASYQVIVDPITAGIRIIIKDKGLPYSPHLVPQYVPPS
                     DIETAGQPGLGSHLIKQTVDEIHFQNMGREGKELHLVKYLPYRSIEEIRTESELAPFP
                     EPVKTVAPPEKKDFSIRRIRPSEVYDVSKLFYRAYGYSYGIDTIYYPEKLAERHADGT
                     IISVVTVTPDERVVGHAALVRDDASSKTAEAAMAVVEPGFRGQGCQSIMITKLVEEAR
                     AVGLAGIYSKAVTNHIYAQKAGQKAGFKRCAVVAGLIPADRSFKGIQAALSQRESVAY
                     GYRVVDDPENMEIFPPAWHRDIIERIYDSTGVRRIFCESPPGAMHDLAGEAEVMVTVV
                     PTYQRAVIEVKRYGEHTVSQVSAILKDLCYQKMEQITLYLNLEDPITGIMCRRFEELG
                     FFFAGILPFSHVGDALLLQYLNNVPIDYSKIKIVDEMGQEILSYVESHDPSRK"
     gene            1957..>4024
                     /locus_tag="PKJ17_03685"
     CDS             1957..>4024
                     /locus_tag="PKJ17_03685"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOD75103.1"
                     /translation="MDREDADAQDVKLLSAEDVRTGLDADTMRRAILENLNFIQARIP
                     VLATRNDWYMALAYTVRDRMLENWVRSTEGLRRTPHAVAYLSAEFLMGPHLGNAMVNL
                     GIREHTAEAVAALGLDLDELLEQEAEPGLGNGGLGRLAACFLDSLATEKIHALGYGIR
                     YEFGIFDQEIVDGWQREITDKWLALGNPWEIARPEITYYVQFGGHTQQYQDEDGRYRV
                     RWVPERVVKGMAYDTPIPGYRVEIVNILRLWKSEACESFDFQAFNTGDYDGAVEEKIA
                     SEMISKVLYPNDEAAAGKRLRLAQQNFFVSCSLQDMIRIHAFVGGKPETLHERFAVQL
                     NDTHPSIAVAELMRLLVDQYRIDWDTAWDATRRTFSYTNHTLLPEALEKWPVPLFQNL
                     LPRHLEIIYEINRRFLDEVRAAYPGDEERARRLSLIDETGDRYVRMAHLATVGSHTVN
                     GVAVLHTELLKRTVMRDFHELTPEKFRNVTNGVTPRRWMVLSNPAMSKLITDAIGDGW
                     VCDCQNNIRRLEPFAGDAGFREAWSEAKRRNKSFLARLIRERTGVVVDPESMFDIQVK
                     RIHEYKRQHLNALHVVSHYLRLKRGHKAEVAPRTVIFGGKAAPGYYMAKLIIKLITSI
                     AEVVNGDPETREKLKVVFLPDFNVKLGQRVYPAADLSEQISTAGKEASGTGNMKFSMN
                     GALTLGT"
BASE COUNT          823 a         1152 c         1200 g          849 t
ORIGIN      
        1 ggtccgtcac ctcgggtttc actcatgcgt accttaccgg agcgttgtgt tgtctcctgg
       61 cgctcgtctt ctcgcttctg ggtagaaagg gcggcggtga tggcggcccc ggatcgaaaa
      121 aagcataacg ttgagcgtta cttcctgctc gggtcgtggg attcgacgta tgaaaggatc
      181 tcctgaccca tctcgtcaac gatctttatc ttactgtaat cgatgggcac gttgttgaga
      241 tactggagga gcaaggcgtc tcccacgtgg gaaaacggga ggatcccggc gaagaagaag
      301 cccagttctt cgaacctccg gcacatgatc ccggtgatgg ggtcctcgag gttaaggtag
      361 agagtgatct gttccatctt ctgataacag agatctttca agatggcgct cacctgcgat
      421 actgtgtgtt ccccgtaccg tttgacctcg atgacggccc tctgatacgt gggaacgacc
      481 gtcaccatca cctccgcctc tcccgcaagg tcatgcatcg ctcccggagg ggattcacaa
      541 aagatcctcc tcacacctgt agagtcgtat atcctttcta ttatgtctct gtgccaggcg
      601 gggggaaata tctccatgtt ttccgggtca tcgaccaccc tgtacccgta cgcgacggac
      661 tcccgctgtg aaagtgccgc ctgtatgccc ttgaaggatc ggtcggcggg gataaggccc
      721 gcgaccaccg cgcaccgctt gaagccggct ttctgaccgg ccttctgggc gtatatgtgg
      781 ttcgtgaccg ccttgctgta tatgcccgcc agtccgacgg cacgcgcctc ttcaacgagc
      841 tttgtaatca tgatgctctg gcatccctga cccctgaagc ccggctcgac gacggccatt
      901 gccgcctcgg cggtcttgct cgacgcgtcg tccctgacga gggccgcatg gccgacgaca
      961 cgctcatcgg gtgtcactgt caccacggaa atgatggtgc cgtcagcatg gcgttcggcc
     1021 agcttctcgg ggtagtagat cgtgtcgatg ccataggaat aaccataggc cctgtagaag
     1081 agtttcgaca catcgtagac ctcggagggc cgtatgcgcc ggatggagaa gtccttcttc
     1141 tccggaggtg ccaccgtctt gacgggctcc gggaacgggg ccagttcgga ttctgtccgt
     1201 atctcctcta tgcttctgta ggggaggtac ttgacgaggt gcagttcctt tccttcgcgg
     1261 cccatgttct ggaagtgtat ctcgtcgacg gtttgcttga tgagatgcga gccgagtccg
     1321 ggctgcccgg ccgtctcgat gtcggaaggc ggaacatact ggggcacgag atggggcgaa
     1381 tagggcagtc ccttgtcctt gatgatgatc ctgatccccg cggtgatggg atcgacgatg
     1441 acctgatagc tcgccttctc gccgggttcg aacgcgtgct tgaccacgtt cacgatggcc
     1501 tcttcgaggg cgatgaggaa catggtcgtg tcccgtttac tgtagccgcg gcgttccatc
     1561 acctccgata caaaggcctg gatcgccgga agatatccga ggtcgttggg aacggtaaga
     1621 ttggagcctt caagcatcgt tgattccatg atcatcccct gttagatagt cgcgaacccg
     1681 atagatcaag tgagagtcat tatactccac agagtctccc atcttcatca aaaaaaacgc
     1741 cgcatggtac caacaaggcg catccctccg gaccacaccc ttcattttcc ctccagtccc
     1801 gaagccattc aagcgatttg acctgcctct cccgtttttc gcctttgaac gttcaagcag
     1861 ctcaagctgt tcaagcggct ccagccgtcc gtattgacac caccacgaga aatcgggtta
     1921 ctatgttgat agcggtgtag accagaggag gttgccatgg atcgggaaga cgccgatgct
     1981 caggatgtga agcttctgtc agcggaagat gtccggacgg gtctcgacgc cgacaccatg
     2041 aggcgcgcaa ttctggagaa tttgaatttc atccaggccc gcattccggt gctggcgacc
     2101 aggaacgact ggtacatggc ccttgcgtac acggtgcggg acaggatgct ggagaattgg
     2161 gtccgttcga cggaagggtt gcgccgcact ccccacgcgg tggcctatct ctcggcggag
     2221 ttcctcatgg gcccccacct gggaaacgcc atggtcaacc tggggatccg ggagcacacg
     2281 gcagaggcgg tggccgcgct cggtctcgat ctcgacgaac ttctggaaca ggaggctgag
     2341 ccgggtcttg gaaacggggg gctggggagg cttgccgcct gtttcctcga ttccctggcg
     2401 acggagaaga tacacgccct cggttacggg atacgctacg aattcggcat cttcgaccag
     2461 gagatcgtgg acggctggca gcgggagata acggacaagt ggctggccct cggcaatccc
     2521 tgggagatcg cccggcccga gatcacttac tacgtgcaat tcgggggtca cacccagcaa
     2581 taccaggacg aggatggacg ctatcgggtc cgctgggttc ccgaaagggt tgtgaagggt
     2641 atggcgtatg atacgcccat tccgggatat cgtgtcgaga tagtcaacat ccttcgtctg
     2701 tggaagtcgg aagcctgcga atcgttcgat tttcaggcct tcaatacggg cgattacgac
     2761 ggggctgtcg aggagaagat cgcctccgaa atgatctcca aggttcttta tcccaacgac
     2821 gaggcggcag cgggcaagag gcttaggctg gcgcagcaga atttcttcgt ttcatgctcc
     2881 ctgcaggaca tgatccgcat ccacgccttc gtggggggga agcccgagac ccttcatgag
     2941 aggtttgccg ttcagctcaa cgacacccat ccgtccatag ccgtcgcgga actcatgcgg
     3001 cttctcgtcg accagtaccg cattgactgg gatacggcat gggacgcgac gagaaggacc
     3061 ttctcgtaca cgaaccacac gcttcttccg gaagccctcg agaagtggcc ggtaccgctc
     3121 ttccagaacc tgctgccccg gcacctcgag atcatatacg agatcaacag acgctttctg
     3181 gacgaagtca gggcggccta tccgggagat gaggagcggg cgcgccgcct gtcgcttata
     3241 gatgagaccg gggacaggta tgtgcgcatg gcgcacctcg ccaccgtggg aagccacacg
     3301 gtgaacggag tggcggtgct tcacacggaa ctcctgaaga ggacggtgat gcgggacttt
     3361 cacgaactga caccggaaaa gttccgcaac gtcacgaacg gggtgacgcc ccgtcgatgg
     3421 atggtgctgt cgaatcccgc catgtcgaag cttatcaccg atgccatcgg cgacggctgg
     3481 gtctgcgatt gtcagaacaa catccgacgc ctcgaaccat tcgccggtga cgccggtttc
     3541 cgggaagcct ggtcagaggc aaaacgccgg aataagagtt tcctggcgcg gctcatcagg
     3601 gagcggacgg gggtagtcgt ggatccggag agtatgttcg atatccaggt caagcggatc
     3661 cacgagtaca aacggcagca tctcaacgcg ctccacgttg tctctcatta cctcaggctc
     3721 aagaggggtc ataaagctga agtcgccccc cgcaccgtca tattcggcgg caaggccgcg
     3781 ccgggctatt acatggcaaa actcatcata aagctgatca cctccatcgc ggaggtggtg
     3841 aacggtgatc ccgagacgcg cgagaagctc aaggtggtgt tccttcccga tttcaacgtc
     3901 aaactcggcc agcgcgtata cccggcggca gacctgtcgg agcagatctc caccgcgggc
     3961 aaggaggcat cgggtacggg gaacatgaag ttttccatga acggcgccct gacgctaggg
     4021 accc
//
LOCUS       DAOPOA010000123         4017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_24275_length_4017_cov_3.948258, whole genome shotgun sequence.
ACCESSION   DAOPOA010000123 DAOPOA010000000
VERSION     DAOPOA010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24275_length_4017_cov_3.948258"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PKJ17_03690"
     CDS             <1..459
                     /locus_tag="PKJ17_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOD75104.1"
                     /translation="DRTKALFARGGSPEVLTVDNHADGPYVYLRLLREDPRRAAEVLE
                     LLRMNEGNSSGVGIACIDEKGNVHADQFWRHHSFGNVRERSFSTIWEDLADPVMAKLK
                     EKKKYVVGRCAACRWLDVCAGNFRVRAEAATGNLWAEDPQCYLTDEEIKR"
     gene            697..1683
                     /gene="hemB"
                     /locus_tag="PKJ17_03695"
     CDS             697..1683
                     /gene="hemB"
                     /locus_tag="PKJ17_03695"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOD75105.1"
                     /translation="MRYPTYRPRRMRKNEKLRNMVRETTLSPDCLVYPVFVKEMSERE
                     VPIDAMPGINQFSVEGVLGEVEKVLEAGVPAILVFGIPEKKDETGSGAYDKNGIVQQA
                     VRAIKARFGEDILVITDVCMCEYTNHGHCGVVKDGYVDNDETLKLLAKSALSHVVAGA
                     DMVAPSDMMDGRVRAIREMIDLHGDYNVPVMSYAAKYASCLYGPFREAAESAPQFGDR
                     KSYQMDPPNSREALREIALDIEEGADIIIVKPALFYLDVLSQARERFNIPLAAYSVSG
                     EYSMIRFAAAQGSLDLKRAVIESTTSIRRAGADIIITYFAKEIGTWAKEHTL"
     gene            1659..2678
                     /locus_tag="PKJ17_03700"
     CDS             1659..2678
                     /locus_tag="PKJ17_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD75106.1"
                     /translation="MGERTYPLRMVAWELTRNCNLSCMHCRASAASGPYEGELSTEEC
                     KAVIDDILSFSEPTVILTGGEPLMRRDIFDIIEYGNDRGLRMVIAVNGTLVDEGVAKR
                     LEASGIKRVSLSIDARDGTGHDKFRGVDGSFRAVTGAAGTLKLTGLPFQINTTVTRLN
                     VDDLDDIYALVRSLGAVAWHVFLLVPVGRGTGLKGKELSAEAYEEVLHRLRAIEKRGE
                     LEMKVTCAPHYYRIVKEDGETPKSAGCLAGKSFMFISHRGIAQPCGYLEIDSGDVRRD
                     GVRTVWEGSEVFRRLRDLTLYRGKCSTCRYVRICGGCRARAYEETGDYLQEEPLCSYR
                     EKNNQ"
     gene            complement(2787..>4017)
                     /locus_tag="PKJ17_03705"
     CDS             complement(2787..>4017)
                     /locus_tag="PKJ17_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOD75107.1"
                     /translation="GYAMNLIFEDAQYSFQTLRATAATAGGAADIGECLKTAYRITEG
                     DDESWYREWFRTAEQRRKEADDLAAQGRMMSAHQEYLKASNYYRTAEFFLHNNPEDPR
                     ILATWREGRESFLKAARLSRAPIIPVEIPFEGTTLPGYRCLVDDRKVKRPLLVIQTGF
                     DGTAEELYYTVAVFALARGYNCLLFEGPGQGRVIREQKIPFRPNWETVITPVVDFALG
                     QEETDPRHIAIMGISFGGYFVPRAMAFEKRIPFCIVNGGVYDFHRTARLTPELEKMLD
                     SAEGSGAIDREVYERMEKSPSLRWAVGNGMFTFHAKTPSEWFRMTRAYTLEGVTDRIT
                     CTMLVVDSEDDRDMPGQSRMLYDALRSPRDYMLFTREEGAEEHCQVGASRISNARILD
                     WLDNAMKAKPMMEGSGK"
BASE COUNT          832 a         1146 c         1231 g          808 t
ORIGIN      
        1 gacaggacga aggccctctt tgccagggga ggatcgccgg aggtccttac cgtcgacaac
       61 cacgccgacg gtccatacgt gtatctgagg cttctcaggg aggacccgcg aagggcggcg
      121 gaggtgttgg aactgctcag gatgaacgaa gggaactctt cgggcgtcgg catcgcctgc
      181 atcgatgaaa agggaaatgt ccacgcggac cagttctgga ggcaccacag cttcggcaac
      241 gtccgggagc ggtctttcag cacgatctgg gaggaccttg cggacccggt catggcgaag
      301 ctcaaggaaa agaagaagta cgtggtcggc cgctgcgcag cgtgcagatg gctcgacgtc
      361 tgtgccggca acttccgcgt ccgcgccgaa gcggcaacgg gcaacctctg ggccgaggac
      421 ccgcagtgtt atctgacgga tgaagagata aaacgataat gatcaatgat caaattcaaa
      481 taaccaaaca atagccaatc gagcaataat caatctccag ccagaaacga cagacgagac
      541 gacccattgg ccacgcacgt gactgttgtc tggtcggctg gaatctggta gatttgtcat
      601 tatcagttcg tggtcactgg cgcctggtct cttggtggtt ttctttccct ggttgctgga
      661 atgtggtcat tggtcattat cttctaagga ggcttcatgc gttacccgac gtaccggccg
      721 aggagaatga ggaagaacga gaaactgagg aacatggtcc gcgaaaccac gctctcgccc
      781 gattgcctcg tctatcccgt ctttgtgaag gagatgagcg aacgcgaggt tcccatcgac
      841 gccatgcccg ggatcaacca gttctccgtc gagggggtgc tcggcgaggt ggagaaggtt
      901 cttgaggcgg gtgtgcctgc catcctcgtc ttcggcatac ccgagaagaa ggacgaaacg
      961 ggcagcggtg cctacgacaa gaacggtatc gtccaacagg cggtgcgggc catcaaggcg
     1021 cgcttcggcg aagacatcct cgtcatcacc gacgtctgca tgtgcgagta cacgaatcac
     1081 ggccactgcg gcgtcgtgaa ggacggctat gtcgacaacg acgagacctt gaagctcctc
     1141 gcgaaaagcg cgctcagtca tgtcgtggcc ggtgccgaca tggtcgcgcc ttcggacatg
     1201 atggacggtc gtgtgcgtgc catcagggag atgatcgacc ttcacgggga ctacaatgtg
     1261 cccgtaatga gctatgccgc gaagtacgca tcctgtctct acggcccctt ccgggaggcg
     1321 gcggagtcgg ccccgcagtt cggtgaccga aagtcctacc agatggatcc ccccaacagc
     1381 cgtgaggccc tgcgggagat cgctctcgac attgaggagg gggcggacat aatcatagtg
     1441 aagccggcgc tcttctacct tgacgtcttg agtcaggcga gagaaaggtt caacatacct
     1501 ctcgccgcgt acagcgtcag cggcgagtac tccatgataa ggttcgccgc ggcgcagggc
     1561 tccctcgatc tgaaaagggc ggtcatcgaa tccacgacga gcatccggcg cgccggagcg
     1621 gacatcatca tcacctactt cgcgaaagag atcggcacat gggcgaaaga acataccctt
     1681 tgaggatggt cgcctgggag ctgacccgca actgcaacct ttcctgcatg cactgccgcg
     1741 cgtcagcggc gtcgggtccc tacgagggcg agctctcaac ggaagagtgc aaggccgtca
     1801 ttgatgacat cctgtccttc tcggagccga cggtcatttt gaccggcggg gaaccgctga
     1861 tgagacgcga tatcttcgac atcatcgaat atggcaatga caggggattg agaatggtca
     1921 tcgccgtgaa cggtaccctt gtcgacgaag gggtggcgaa aaggctcgag gcctcgggta
     1981 taaagagggt gagcctcagc atcgacgcaa gggacggaac cggtcacgac aagtttcgcg
     2041 gtgtcgacgg gtccttccgg gccgtgaccg gggcggcagg gaccctcaag ctgacgggtc
     2101 tgcctttcca gataaatacc acggtgacga ggctcaatgt cgatgacctc gacgatatct
     2161 atgcgttggt caggtccctc ggggctgtcg cctggcacgt ttttctcctc gtgcctgtcg
     2221 gcaggggaac gggtctcaag ggaaaggagc tctccgccga ggcctatgag gaggtgctcc
     2281 accggcttcg ggcaattgaa aagaggggcg agctggagat gaaggtgacc tgtgccccgc
     2341 actattaccg catcgtgaag gaagatgggg agacgccgaa aagcgcaggg tgccttgccg
     2401 ggaagagctt catgttcatc tctcaccgag gcatcgccca gccctgcggt taccttgaga
     2461 tcgattcggg cgatgtgcga agggacggtg tgaggactgt ctgggagggc tccgaggtgt
     2521 tccggcgcct gcgcgatctc acgttatatc gtggaaaatg ctcgacctgc cgctatgtcc
     2581 gcatctgcgg cggctgccgc gcccgcgcct acgaggagac gggagattac ctccaggaag
     2641 agccgctgtg ttcgtacagg gaaaagaata accaatgatc aaatcccaat aaccagacaa
     2701 gagcgaattg gccgaagatc gacagtcgaa cgaaaagcgt caaagctccc atccggcagg
     2761 atacggtgca gttgttcatt gagccttcat ttcccgcttc cctccatcat cggttttgct
     2821 ttcattgcgt tgtccagcca gtcgaggatg cgcgcgttcg atatacggga ggcgcccacc
     2881 tggcagtgtt cctccgcccc ttcttccctg gtgaagagca tgtaatcccg gggagacctg
     2941 agcgcgtcgt acagcatccg tgactggccc ggcatgtccc tgtcatcctc ggagtcgacg
     3001 acgagcatcg tgcaggtgat cctgtctgtc accccttcaa gcgtgtaggc ccgtgtcatc
     3061 ctgaaccact ccgacggcgt cttcgcgtgg aaggtgaaca tgccgttgcc gaccgcccat
     3121 cggagggaag gactcttttc catcctctcg taaacctccc tgtcgatggc cccggagccc
     3181 tccgccgagt cgagcatctt ctccagctcg ggggtgaggc gggcagtcct gtgaaagtcg
     3241 tagacgccgc cgttcactat gcagaagggg atccttttct cgaaggccat ggcccgcggc
     3301 acgaaatacc ccccgaagct gatccccatt atcgcgatgt gccgggggtc cgtttcctcc
     3361 tgtcccagag cgaaatcaac aacaggtgtg atcacggttt cccagttcgg gcggaatggt
     3421 atcttctgtt cacgaatgac gcgtccctga cccggcccct cgaacagcag acagttgtat
     3481 cctctcgcga gggcgaatac ggccacggta taatagagct cctccgcggt tccgtcgaaa
     3541 cctgtctgga tgacgagaag cggtcgcttt accttccggt catccacgag acagcgatac
     3601 cccgggaggg tcgtgccttc gaaagggatc tccacgggaa ttatcggtgc ccgggaaagt
     3661 cgcgcggcct tgagaaagct ctcgcggcct tctcgccagg tggcaagtat tcgggggtct
     3721 tcagggttgt tgtggaggaa gaactcggcg gtcctgtaat agttggacgc cttcaggtat
     3781 tcctggtgtg cgctcatcat tctgccctgt gcggcgagat catcggcttc cttccttcgc
     3841 tgctcggccg tcctgaacca ttcgcggtac cagctctcgt catcgccttc cgtgatgcgg
     3901 taggcggttt tgaggcattc accgatgtcg gcggcgcccc cagccgtcgc cgctgttgcc
     3961 ctcaaggtct ggaaagagta ctgcgcgtcc tcgaagataa ggttcatggc atacccc
//
LOCUS       DAOPOA010000124         4012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_24313_length_4012_cov_2.688400, whole genome shotgun sequence.
ACCESSION   DAOPOA010000124 DAOPOA010000000
VERSION     DAOPOA010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24313_length_4012_cov_2.688400"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKJ17_03710"
     CDS             complement(<1..669)
                     /locus_tag="PKJ17_03710"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HOD75108.1"
                     /translation="MEGHEELERPFDFRQCISIIKSTGEKAADLKELREVIARVSDDS
                     IFHHTYQYFLKEHILEYTNDLAHWAGESLEERALAEQLSNIDPYSFGSIADLREALVG
                     VIDSYLERFPEPRYALPGDEFFFNETVTFVFPVGVYAKNLAEFLLALRFVDSGSIYYH
                     FYEARMRVPAGTDDFSRWVDEALGKRELAEKIRAIDPFMHNTEEIRAYIAGIVMEEVS
                     RDMEK"
     gene            complement(712..972)
                     /locus_tag="PKJ17_03715"
     CDS             complement(712..972)
                     /locus_tag="PKJ17_03715"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOD75109.1"
                     /translation="MRFLILIACIVIVVAIFSTQNSQPVSVSFFVWNFQASLAIVVFL
                     SVICGLVLGVLASFFLGLTRKRKAAAGKGPGTTPAEPDGTGS"
     gene            complement(1013..2224)
                     /locus_tag="PKJ17_03720"
     CDS             complement(1013..2224)
                     /locus_tag="PKJ17_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75110.1"
                     /translation="MSDFYQTGVVPTLHRLGKDNLEEIESRLRRYAQDRPIALVLPSL
                     YSELSGAALGGIVEELKKVQYIKEIVVTLGPASDSEFQNARRFFSALPQKVCVIWNNG
                     KRMKKLYRAIEAEDLTTGLEGKGRSVWMAYGYVLARREFNAIALHDCDILTYSRDMLA
                     RLCYPVTNPSLDYDFCKGFYTRVTDKMHGRVTRLLVTPLIRALVKIIGFHPLLVFFDS
                     FRYILAGEFSMDVNLARINKIPGDWGLEVGTLAEVYKNTSINRVCQVDIAENYEHKHQ
                     ELSPEDASKGLNKMCVDICKSIFRTLTSEGIVFSDGFFKTLFATYARTAQDHLKRYED
                     DASINGLRFDRHEESLSVETFTKGIKTAAEVIMKDPLGVPLISSWDRVTAALPDILDR
                     IREAVEEDNRS"
     gene            2408..3235
                     /locus_tag="PKJ17_03725"
     CDS             2408..3235
                     /locus_tag="PKJ17_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOD75111.1"
                     /translation="MPVRRAVVFTDLDGTLLDARTYSFDPALPALGMLRERHVPVVIC
                     SSKTRAEIEHYRAKLRNADPFISENGGAVFVPHGYFRADAVSPARPEGGDEGPHLVLR
                     LGARYQDLRRALRELRGEGFDVRGFGDMTAEEIAAATGLPLNEAVMAAKREFDEPFLF
                     GGDAVMGDKLRASIERKGFRVTQGLFHHILGESDKGKAVSILTGMFRREYGEVTTVAL
                     GDSPNDLPMLQAVDRPIIVEKHGGGYDRVLVAEDFDRARGAGPDGWNSAIMRLLKDL"
     gene            complement(3259..>4012)
                     /locus_tag="PKJ17_03730"
     CDS             complement(3259..>4012)
                     /locus_tag="PKJ17_03730"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOD75112.1"
                     /translation="LIMAFLYAAYIIGRTFLQPSMAPDYEVPRSDPVSIVKELLVYVM
                     PLTSVVALVLGPIYMGIATPTEAASLGCVGALTLSIVYGKLTFSIFSEALAATLRTSS
                     MIFLIIAGSVGYSQIISFSGAGAGLISAVVSLNLPDVLLITILLSMLLLLGCFMDQIA
                     MMMVTVPLFMPIVYQANIDPLWFGIMMLIALDIGFTSPPFGLLLFVMKGITSEDITML
                     DIYKATIPFIACNLFTLTLILAFPRIVMVFLK"
BASE COUNT          851 a         1107 c         1232 g          822 t
ORIGIN      
        1 cttttccatg tccctgctca cttcctccat gacgatcccg gcgatgtagg cccgtatctc
       61 ctctgtattg tgcatgaaag ggtcgatggc cctgatcttt tccgccaact ccctctttcc
      121 cagcgcctca tcgacccacc gggagaaatc atcggtgccg gcgggaacgc gcatgcgcgc
      181 ctcgtaaaaa tggtagtaga tgctgccgct gtcaacgaat ctcagggcaa gaaggaattc
      241 ggccaggttt ttcgcgtaga ccccgacggg gaagacgaag gtgaccgtct cgttgaagaa
      301 gaactcgtct cctggaaggg catagcgagg ctcggggaac ctttccaggt aggaatcgat
      361 cacccccacg agggcttcgc gaaggtccgc gatggaccca aaggaataag ggtcgatgtt
      421 cgacagctgc tccgccagcg ccctctcctc gaggctttcc ccggcccaat gtgcgagatc
      481 gttggtgtac tccaggatgt gctccttcag gaaatactgg tacgtgtgat ggaatatgga
      541 atcatcgctt accctggcga tcacctcccg caattccttg aggtccgccg ccttctcgcc
      601 cgttgacttt attatgctta tgcactgcct gaagtcaaaa gggcgctcca attcttcgtg
      661 tccttccata cgtttcacct caccgatgag cgcggtcccg gagacccgcg atcacgaccc
      721 ggtcccgtcc ggttccgccg gggttgttcc gggccccttc ccggccgccg cttttcgttt
      781 cctggtcagc cccaggaaga atgacgccag cacaccgagg accaggccgc atatgaccga
      841 gagaaagacc acgatggcca gagaggcctg gaagttccag acgaagaagg agacggagac
      901 cggctgcgag ttctgggtgg agaaaatggc cacgacaatg acgatgcagg cgataagtat
      961 gagaaaacgc atcttgcctc cctcggcccc ttcccggccg gcctcgcgtt cttcaggacc
     1021 ggttatcctc ctcgaccgcc tcccgtatcc tgtcgagtat gtcgggcaat gccgccgtca
     1081 ccctgtccca gctcgagatg aggggaactc ccagggggtc tttcatgatc acctcagcgg
     1141 cagtcttgat gcctttcgtg aaggtttcca cggagagact ttcctcgtga cggtcgaaac
     1201 gcaggccgtt gatggaggcg tcatcctcgt acctcttcag gtggtcctgt gcggtgcgtg
     1261 cataggtggc aaagagtgtc ttgaaaaaac cgtcggaaaa gactattccc tcggaggtca
     1321 gggtccggaa tatggacttg cagatatcca cgcacatctt gttgaggcct ttcgaggcgt
     1381 cctcggggga aagctcctgg tgcttgtgtt cgtagttctc ggcgatgtcg acctggcaga
     1441 cgcggtttat ggaggtattt ttgtagacct ccgcgagtgt tcccacctca aggccccagt
     1501 cgccgggaat cttgttgatg cgggcgaggt tcacgtccat ggagaactcg cccgccagga
     1561 tatagcggaa actgtcgaag aagaccagga gagggtgaaa tccgatgatc ttgacaaggg
     1621 cgcgtatgag gggggtcacg aggagccggg tgaccctccc gtgcatcttg tccgtgactc
     1681 tcgtatagaa gcccttgcag aaatcgtagt cgaggctggg gttcgtgacg ggatagcaga
     1741 gcctcgcgag catgtcgcgc gaataggtga ggatgtcgca atcgtgcagg gcaatggcgt
     1801 tgaactccct ccgcgccaaa acgtagccgt aagccatcca cacggagcgg cccttgcctt
     1861 cgaggcccgt ggtaaggtct tccgcctcga tggcccggta cagtttcttc atcctctttc
     1921 cattgttcca tatgacgcac accttctggg ggagggcgga gaagaaacgc cgggcgttct
     1981 gaaactcgct gtccgacgcg ggacccaggg tcacgacgat ctccttgata tactgaacct
     2041 ttttcagttc ttcaacgatc ccaccgagtg cagcgccgga aagctcgctg tacagggagg
     2101 gaaggacgag tgcgatgggc ctgtcttgag cgtatcgcct gagcctcgat tcgatctcct
     2161 cgagattgtc ctttcccagt ctgtgcagcg tggggacaac gcctgtctgg taaaagtcgg
     2221 acatgctcac ctcctggaat gtagtctagc actctatccg agaagcggtg gaaaatctgt
     2281 aaactatttt acactctcct gaaaccgcta tatggtagca tatcataagt atgataatac
     2341 gtcacaaaaa agggtcgcgg gctcacatgt gccgttcgcc ttcgggcggg ggtgccggaa
     2401 aggctgtgtg ccggtgagaa gagccgtcgt gttcaccgac ctcgatggaa ccctcctcga
     2461 cgcgcggacc tattcctttg atccggccct gcccgccctc ggcatgctca gggagagaca
     2521 tgtccccgta gtgatctgtt ccagcaagac acgggccgag atcgagcatt atcgcgcaaa
     2581 actgcgtaac gccgaccctt tcatctcgga gaacggaggc gcggtcttcg tgccacacgg
     2641 gtatttcagg gccgacgcgg tgtcgccggc gaggcccgaa ggaggcgacg aggggccgca
     2701 tcttgtcctc cggcttggcg ccaggtatca ggacctgcgc agggccctga gagagcttcg
     2761 cggcgagggg tttgacgttc gcggattcgg agatatgacc gccgaagaga tcgccgccgc
     2821 gaccggcctg cccctcaacg aagcggtcat ggcggcaaag cgggaattcg acgaaccttt
     2881 cctcttcggg ggagacgcgg tgatggggga taagctgcgg gcctccatag aaaggaaggg
     2941 cttcagggtc acgcaggggc ttttccatca tatcctgggc gaaagtgaca agggtaaggc
     3001 tgtttccatc ctcaccggaa tgttccgcag agaatatggc gaggtgacga cggtggccct
     3061 gggggacagc cccaatgacc tgccgatgct ccaggctgtc gaccgcccga tcatcgtcga
     3121 aaaacacggc gggggctacg accgggtgct tgtagcggag gatttcgacc gggcccgggg
     3181 agcgggaccg gatggttgga acagtgcgat aatgagactc ctcaaggacc tgtgaatcct
     3241 gtcccacggt ccttcacgtc atttcagaaa aaccatcact atgcgcggga acgcgaggat
     3301 gagagtgagg gtgaagagat tgcaggcaat gaaggggatt gtcgccttgt aaatgtccag
     3361 catggtgata tcttccgagg ttatgccttt catcacgaaa aggaggaggc cgaagggcgg
     3421 cgaggtgaag ccgatgtcga gggcgatgag catcatgatc ccgaaccaga gcgggtctat
     3481 gttcgcctgg tagacgatgg gcatgaagag ggggaccgtt accatcatca tggctatctg
     3541 gtccatgaaa cagccgagca gaagcagcat cgaaagcagg atcgtgatga gaaggacgtc
     3601 gggcaggttg agggagacga cagcggagat gaggcccgct ccggcgccgc tgaaggaaat
     3661 gatctggctg tacccgacgg aaccggctat tatgaggaag atcatcgaac tcgttctcag
     3721 cgtggccgcc agggcttccg agaatatcga gaatgtgagc tttccgtaca cgatcgaaag
     3781 ggtcagggct cccacgcatc ccagggaggc ggcctcggtg ggtgtggcta tccccatgta
     3841 tatcgggccc aggacgaggg ctacgacgct tgtcaaaggc atgacgtaaa cgagaagctc
     3901 cttcacgatg ctcacggggt ccgatctcgg gacctcgtaa tccggggcca tcgagggctg
     3961 gaggaaagtc ctccctatga tataagcggc gtagagaaag gccatgatga gt
//
LOCUS       DAOPOA010000125         4002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_24390_length_4002_cov_2.844692, whole genome shotgun sequence.
ACCESSION   DAOPOA010000125 DAOPOA010000000
VERSION     DAOPOA010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24390_length_4002_cov_2.844692"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PKJ17_03735"
     CDS             complement(<1..364)
                     /locus_tag="PKJ17_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75113.1"
                     /translation="MEDTKKTKQQQTGAGQKGGKGKAERETNPFIDSMTLINKSYVYE
                     FASEAYCRDHGRTRREVVGNSVTQIWGESNFKFIKTHLDRCFAGNVVRYENWLEFPGE
                     EPKFYRVTYSPYYNPAGYV"
     gene            complement(693..1520)
                     /locus_tag="PKJ17_03740"
     CDS             complement(693..1520)
                     /locus_tag="PKJ17_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOD75114.1"
                     /translation="MDGKDYYEVLEISVEATDRQVKEAYRRLAFQYHPDRNGSDPGAV
                     ERMKSINEAYAVLSDPAKRQRYDNLRQSYGTSAYDRFREGYSENDIFRGSDINQIFEE
                     ISRSFGFRGFEDIFREAYGSGYRTFEFRNGGVFGKGFIFTGRMGEGAFGPGAKQGIGS
                     RVLGKLAGYALKKMTGIGTDNGGDRYDSLRLTTEQAKNGGKVPYADKGSSRNILINVP
                     PGVSQGQTIRLKGMGSGGGPFTAPGDLYLHVEIAKPFMRTIRNLLRKKTDGLKPSGR"
     gene            complement(1607..1948)
                     /locus_tag="PKJ17_03745"
     CDS             complement(1607..1948)
                     /locus_tag="PKJ17_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75115.1"
                     /translation="MEIRIRRVGSRTLTGNVSGRVNTYRVDQDGREFLLTRTSNTLAS
                     NIGIAGQRGILYVETEDNKVHHQVVAPSGACGLNTDDEVVEGLSPLALRAVLMVDKCD
                     EPGEITITREE"
     gene            complement(2008..2181)
                     /locus_tag="PKJ17_03750"
     CDS             complement(2008..2181)
                     /locus_tag="PKJ17_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75116.1"
                     /translation="MMFGVEYECRDCGRAFRVDEEEKREPRCPGCEGGNVTIRQARPL
                     PQWIMRKKEAAPT"
     gene            complement(2276..3835)
                     /locus_tag="PKJ17_03755"
     CDS             complement(2276..3835)
                     /locus_tag="PKJ17_03755"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD75117.1"
                     /translation="MEQTDQTLTDFLLDPRNYPERTRAVSHVETHISHVFIGDRFVYK
                     VKKAVNFGFLDFSTLKKRRFFCEQEVVLNSRLAKDIYLGVQPFYRSHAGFSFARKGRG
                     LPVEYAVRMKRIPLERLLFKLIGEGRPLYGSLEDVGRTLARFHEDIVPYRGRGFGGVE
                     SIGAATEENFEQIRPFTALTVGRKTLASIARYTRAFIVDHRARFAERRRKGFIREGHG
                     DLHCQHVCLEHPPVIFDCIEFNRGFRVIDILEDIAFLFMDLEYRGRFDLSARLHKAYF
                     AHQPKALDEELLRFYKVYRAVVRGKVEGFAARGVEDRSQREGAVARARDYFGLADHYI
                     NHFQKPFNPVVFMGLSGSGKSTIARDFSPNAVILRSDEMRKEITGVRKGRHAYSSFGE
                     GIYTPELTNRIYRLMLDRATEHAGMGKKVIVDATYLRAEQRRDFHETCIERGLNPFFI
                     HCFASEAVLKERIRKRMEAGTDISDADIAVLDHQLEHLEDPEELPRYRTLRVNTEDAI
                     HNIVHALREFL"
BASE COUNT          767 a         1261 c         1051 g          923 t
ORIGIN      
        1 tcacatatcc tgccgggttg tagtacggag agtacgtgac gcggtaaaat ttcggctcct
       61 cgccgggaaa ttccagccag ttctcatagc gcacgacatt gccggcaaaa caccggtcca
      121 ggtgcgtctt gatgaacttg aagtttgatt cgccccagat ctgggtaact gaattcccga
      181 ccacctccct cctggtcctc ccatggtccc ggcaataagc ctcactggca aattcgtaca
      241 cgtaggactt gttgatgagt gtcatggaat cgatgaaagg atttgtttcc cgctcagcct
      301 tgccttttcc ccccttctgg cccgctcccg tttgctgctg tttcgttttc ttcgtgtctt
      361 ccattcctcc ccctttttgc ttttatatag acgtactgtt gaaggcacac gaaagcaccg
      421 caatgcaacc agcgacggaa taagtgaaat cccagggaac gaactgcgca gactacacga
      481 ccactggcgt cttctctacc ccccttttct ttgaatagtg aatgatacag tgcccttatg
      541 acttatccgg cattgtattt ttttgaggat accaccccgt ccggaaaaaa cacaacacaa
      601 aaaatggggg ccgatccctt accggcgcca ggaaaggggc atcatgcgaa aaagaccgct
      661 cggaacggct gcgtgtaccc gtgaagaacc ggtcagcgac ccgacggctt caatccatcc
      721 gtcttcttcc tgagaaggtt cctgatcgtc cgcataaaag gtttggcgat ctccacgtgc
      781 agatagagat ctccgggtgc cgtgaacggt ccgcccccgg atcccatccc cttaaggcgg
      841 atggtctgtc cctgggagac gccgggaggg acgttgatga gaatgtttcg cgagcttccc
      901 ttgtccgcat acggcacttt gccgccattc ttcgcctgct cggtggtgag ccgcagggag
      961 tcgtaccggt cgccgccgtt gtcggtcccg atgcccgtca tctttttcag ggcgtaaccc
     1021 gcaagctttc ccaggacgcg ggagccgatt ccctgcttgg cgccgggccc gaaggcgccc
     1081 tcgcccatgc ggcccgtgaa tatgaaacct ttgccgaaaa caccgccgtt cctgaactcg
     1141 aaggtccggt agccgctgcc gtacgcctct ctgaagatgt cttcaaaacc cctgaacccg
     1201 aaggaccgtg agatctcctc gaagatctgg ttgatgtcgg acccgcggaa gatatcgttt
     1261 tcggagtaac cctcgcggaa cctgtcgtag gcggaggtgc cgtagctttg cctcagattg
     1321 tcataccgct gtctcttggc cgggtcggac agaacggcat acgcttcgtt tatgctcttc
     1381 atccgctcca cggcacccgg gtccgacccg tttctgtccg ggtgatactg gaacgcaagc
     1441 ctgcgatatg cctccttgac ctgcctgtcc gtggcctcga cgcttatttc gaggacctcg
     1501 taatagtctt ttccgtccat attccttccc tctgcagggc ctgccatgcc ccgatcgggg
     1561 actcgagggc aggtgctgtt tccccgtgtc cgatcggaac gccctgttat tcttcccttg
     1621 ttatcgtgat ctcgcccggc tcatcgcact tgtcgaccat gagcaccgcg cgaagcgcga
     1681 ggggcgagag cccttcaacc acctcgtcat cggtgttgag tccgcaggcc ccactcgggg
     1741 ccaccacttg atggtggacc ttattatcct cggtctcaac gtagagaatg cccctctgcc
     1801 cggcaatgcc gatattgctc gcaagcgtgt tggaggtccg ggtaagcaga aattccctgc
     1861 cgtcctgatc gaccctgtat gtgttcaccc ttcccgacac gttgcccgtc agggttctcg
     1921 aacccactct cctgatcctt atttccatgc gagacgcgtc ctgatcaaag atcttcacga
     1981 atggcaacgc caccctcttt tctactttca ggtcggcgcg gcctctttct tgcgcatgat
     2041 ccactgcggc agcggacggg cctgtcttat cgtcacattc ccgccttcgc agcccgggca
     2101 cctcggctcc cttttctcct cttcatcgac ccggaaggcc cggccgcagt ccctgcattc
     2161 gtactcgacg ccaaacatca tatgtcacct ccatgaagcg gagcttatgt gagtatagta
     2221 cttccgcggc cctttttcca gacccccggc cttcaccccc ggggaggcgc tgttcctaca
     2281 ggaactctct cagcgcgtgg actatgttgt ggatggcgtc ctccgtgttg acgcggagag
     2341 tgcggtagcg aggaagctct tccgggtctt caaggtgttc gagctggtga tcgagcacgg
     2401 cgatgtccgc gtcggagata tccgtacccg cctccatcct ctttctgatc ctctccttca
     2461 ggacggcctc gctggcgaag cagtgaatga agaaaggatt aagccccctc tcgatacagg
     2521 tctcatggaa atcccgtctt tgctccgccc tgaggtaggt cgcgtcgacg atcaccttct
     2581 ttcccatccc ggcgtgctcg gtcgccctgt ccagcatcag gcggtagatc cggttggtga
     2641 gctcgggagt gtagatccct tctccaaagg aactgtacgc gtgcctgccc ttcctcacgc
     2701 ccgttatctc cttcctcatc tcgtccgacc tgaggatcac ggcgttgggt gagaaatccc
     2761 tcgcgatcgt cgatttgccc gatcccgaca gacccatgaa gaccaccgga ttgaatggct
     2821 tctggaagtg gttgatgtaa tgatcggcaa ggccgaagta gtcccgggcc ctcgccacag
     2881 ccccttccct ttgcgacctg tcttccacgc ccctggcggc gaatccctcc accttgcccc
     2941 gcacgacggc gcggtacacc ttgtagaacc ggagcagttc ctcatcgagg gccttcggct
     3001 ggtgtgcgaa ataggccttg tggagcctcg ccgacaggtc gaaccttccc cggtattcga
     3061 ggtccatgaa gagaaaagcg atgtcctcca ggatatcgat gaccctgaaa cccctgttga
     3121 actcgatgca gtcgaatatg acgggaggat gctccagaca gacatgctga cagtggaggt
     3181 ccccgtggcc ttcccggatg aaccccttcc tccttctctc agcaaacctg gccctgtggt
     3241 cgactatgaa cgcgcgggta taccgcgcga tcgaagcgag ggtcttcctg ccgacggtca
     3301 gtgccgtgaa gggccttatc tgctcgaaat tctcctccgt ggcggcgcct atggattcga
     3361 cacctccgaa cccgcgcccc ctgtacggca cgatgtcctc gtggaagcgg gccagcgtcc
     3421 tgcccacgtc ttccagcgag ccatacaggg gccgcccttc gccgatcagc ttgaaaagca
     3481 ggcgctccag ggggatgcgc ttcattctta ccgcgtattc cacaggcaga ccccgtccct
     3541 tccgggcaaa actgaacccg gcatggctcc tgtaaaaggg ctgaacgccg agataaatgt
     3601 cctttgcaag acgggaattg agcaccacct cctgttcgca gaagaatcgg cgcttcttca
     3661 acgtcgagaa atcgagaaaa ccgaaattca ccgctttctt cactttgtaa acaaacctgt
     3721 caccgatgaa cacgtgtgat atgtgcgtct ccacgtgcga gacggcacgc gttctctcgg
     3781 gatagtttcg gggatcaagg agaaaatcgg tgagtgtctg atccgtctgt tccatgtgac
     3841 gctctcggct atactctatg ctagtatcta ttagccgcga aagcaaggcc ctgccacggc
     3901 ggcccgacaa gatcccgggc acgaaaaaaa ggccggctcg tgaagccggc cttcaagttg
     3961 atctttacgg gtcccgccgg ggaaggatgc agacagcctt cc
//
LOCUS       DAOPOA010000126         3997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_24435_length_3997_cov_2.976662, whole genome shotgun sequence.
ACCESSION   DAOPOA010000126 DAOPOA010000000
VERSION     DAOPOA010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24435_length_3997_cov_2.976662"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(559..1047)
                     /locus_tag="PKJ17_03760"
     CDS             complement(559..1047)
                     /locus_tag="PKJ17_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75118.1"
                     /translation="MEEERNDVLEEIHAEATDVIGGLVLAETWLTGGWDGTLEKTTHV
                     MPRLAGAVSAARQAMERILELAGDGRQKLREGTIMEQQSREGERGKTVYSVLAEIENQ
                     LKLIRRVTYDHFLEDDIDTERRDEVLGVHNTLQDIEMKIAMLIKGDELEAIKAPAALQ
                     IW"
     gene            complement(1194..1628)
                     /gene="tsaA"
                     /locus_tag="PKJ17_03765"
     CDS             complement(1194..1628)
                     /gene="tsaA"
                     /locus_tag="PKJ17_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOD75119.1"
                     /translation="MNETIFEIRRIGLVRSRLSSREEAPHQGSEGAPDAWIDVDPAFA
                     EGLQGIAVGGDIIVVTWFHQARRDLLKVHPRGDRKRPTTGVFCTRSPDRPNPLGLHRV
                     KVLEITGNRIKVAPLEAIDGTPVVDIKPVLPGQADTIADEDK"
     gene            2162..2476
                     /locus_tag="PKJ17_03770"
     CDS             2162..2476
                     /locus_tag="PKJ17_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75120.1"
                     /translation="MGYGLGTLNKNADPGHLHSGDYYDFVQIAYGYEKLLSGKFNILV
                     EPFVAIVNRPQSGLDGGLTLNGRHYFGHENYTGLFVTAGFGGAYTSVDLEDQGTHGLF
                     IL"
     gene            complement(2698..>3997)
                     /locus_tag="PKJ17_03775"
     CDS             complement(2698..>3997)
                     /locus_tag="PKJ17_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75121.1"
                     /translation="SGCRGEYRLITSGGREITTSVSACPIQLQGVECLCLVISDLTEQ
                     RSFEQQLRRAQKLEALGTLAGGIAHDFNNVLAGIIGFAEMVLEDTSPDRPEHRRLTMV
                     LKAAHRGRDLVKQILLFSRRGEQDRKPLALVEVVEDTLKLLGPAFPSTIEILWKGTAD
                     DCRILADAAQMHQVVANLCTNAVHAMRETGGILQIVASRAIVAEGTPPPLPEMAPGEY
                     VVLEVSDSGCGMDPETMEQMFDPFFTTKGEGEGTGLGLSVSHGIVKGHGGYIAVESEP
                     GIGSRFLVYLPGFAEGADIQVESASSSPLGGRERILIVDDEDILVELNRQRLSGLGYD
                     VTATTSSLDALQMFGRAQDSFDLVITDETMPHMTGMDLAAELLKAKPGIPIILCTGHS
                     DKVSPERAWEAGIQKLLMKPFDKRELAEAVRQLLDKDKKS"
BASE COUNT          797 a         1174 c         1044 g          982 t
ORIGIN      
        1 gttggttaaa tacacctgtc gcaatgtcat gactccctcc tcctatatgt cctggccaca
       61 ccggtaagca ctgggtaagc agaatatttc caattctatc atacaccata gggattagtc
      121 tatctgtaac tacctatact gtcaagcttt atattgctta cccatacggg gtacaacacc
      181 atcatacccc aataaaaggg acttaaaatc cctcgctcct cgcgagtgta cgggttcgat
      241 tcccgtccca ggcattgata gtaaagggag acagtgccct ttcgattttt acattctctt
      301 ttccaccagg cttgaccgag taatgatggt cgctagacgg tgttggccag atgctactcc
      361 gcttcatccc caaataatac gcaatgatac tgaactcctt gatgtttaat caaagccgcg
      421 caataatggc tggatgtgga gcttgtacct ggatgaattg atcgtgggtt cgtggcacgg
      481 agcgtggctg cataacagag agaccccacg caccgggccg cgttgaactg taacgcataa
      541 gatgtcaacg tacgaccctc accatatctg tagggccgcc ggggccttta tagcttcgag
      601 ttcgtcgccc tttatgagca tggcgatctt catttcgata tcctgaaggg tgttgtggac
      661 tcccaggact tcgtccctgc gctcggtgtc gatgtcgtct tcgaggaaat ggtcgtaggt
      721 gacgcgccgt atcagcttca actggttctc gatctccgcc aggaccgagt agacggtttt
      781 gcctctttcc ccttctcgcg actgctgttc catgatggtc ccctcacgca gcttctgccg
      841 cccgtcgccg gcaagctcca ggatcctctc catcgcctgt ctcgccgcgc taacagcgcc
      901 ggccagtctg ggcataacgt gcgttgtctt ctccagggtc ccgtcccaac ctcccgtcag
      961 ccatgtctcg gccaggacaa ggccgcctat cacatccgtt gcttcggcgt ggatctcttc
     1021 cagaacgtcg ttcctttcct cttccatggt ctcctccttt ccgttgccgc tggctcgctg
     1081 atcgcgtcct gcaggtcaac gagcatatcg cgcgggatgc ggtgcgtgac gccatgacct
     1141 tgatctgtcg ctaaagtctc ataactctca aaacggtcag cgagaaagag cagttatttg
     1201 tcctcgtcgg cgatcgtgtc cgcttgtccc ggcagcacgg gcttgatgtc gacgaccggt
     1261 gttccgtcaa tggcttccag gggcgccacc ttgatcctgt tcccggtgat ctccaggact
     1321 ttgacgcgat gaaggccaag cgggttcggc ctgtcgggag agcgcgtaca gaagaccccg
     1381 gtcgtgggtc tcttcctgtc cccgcgggga tgcaccttca atagatcgcg acgggcctgg
     1441 tgaaaccacg tcaccacgat aatgtcacca cccactgcga taccctgaag gccctccgca
     1501 aaggcagggt ccacgtcgat ccacgcgtcg ggggcgcctt cgcttccctg gtggggggct
     1561 tcctcccggc ttgacagcct ggagcgcacc agcccgatcc gtcttatctc aaaaatggtc
     1621 tcgttcattc tctcggagtg ccgccgccat cactttggat gggcagactt tagattattc
     1681 tcttacaact cttgttgttt atcaatataa ttagccgcgt cgggaaatgc tgaccggaaa
     1741 caccctgtct gccccgaaac actcatccac ttatcccggc ctgtggacat agcgtggtcg
     1801 cgagaggtgt ccgccagggg atcgtgacgg ttcccgatgc tttttcagca gcgctctaac
     1861 tggtggacat cacgttgttt tcatgacata ttcgaccgct gggcctttgt cctggtcact
     1921 ggctcatgat actattacaa aagtgaccgc aagggtaagg agcaggatct tcgggaagag
     1981 acgggaacga tacctgccgg gagattggcc atgccgataa gagcaccctt accctgaggt
     2041 attgtgggag acaaatatgc gtattaacgt ccgttcgcgc tttctcattc ttgttttact
     2101 cctggtcatt ccgctatcgt tcttaccctc caccgctctc gctgatcaac aggagatctc
     2161 cgtgggatac ggcctcggga cccttaataa gaatgcggac cccggtcacc tccacagcgg
     2221 tgattactat gacttcgtgc agatcgcgta tggttacgaa aagttgcttt ccggcaaatt
     2281 caacatcctt gtcgagccct ttgttgccat cgtcaaccga ccacagagtg gtcttgacgg
     2341 cggtttgacg ttgaatggga ggcattattt cggtcacgag aactacacgg gccttttcgt
     2401 cacggcgggt ttcggtggtg cttatacgag cgttgatctt gaagatcagg gaactcacgg
     2461 gttatttatt ctttagtgcg gtatcggtta tacgtggcgg aggcttttcg tggaatacag
     2521 gttcaaacac tattcaaacg gcggaaccgc aaaccccaat aactctgtga attcgagcgt
     2581 ggtgggtatg ggttttgcct tctgatgggc cgggtagatg acaaggatct caaggaactg
     2641 gtacctttct cctggcggag gcccaaaggg gagatggagg aaacgacggc ttgcctgtca
     2701 gcttttctta tccttgtcga gcaactggcg gaccgcctct gccagttcgc gcttgtcgaa
     2761 gggtttcatg aggagctttt ggattcctgc ctcccatgca cgttccggtg agaccttgtc
     2821 gctgtgaccc gtgcaaagaa tgatcggaat gcccggttta gctttcagca gttccgccgc
     2881 aaggtccatc cccgtcatat gcggcatggt ctcgtccgtt atgacgaggt cgaagctgtc
     2941 ctgtgcccgc ccgaacatct gcagggcgtc caggctgctt gtcgtcgcgg tcacatcata
     3001 gcctaaaccg ctcaatcttt gtcggttcaa ctcaaccagg atgtcctcgt cgtcgacgat
     3061 gaggatgcgc tccctcccgc ccaatgggga agaagacgcg ctctcaacct ggatgtccgc
     3121 accctccgcg aacccgggca aatacaccag gaacctcgac ccgattcccg gttcgctctc
     3181 aaccgctata tatccgccat gacccttcac gatgccgtgg gagaccgaca gtcccagacc
     3241 cgtcccctcc ccttctccct tcgtagtgaa gaagggatcg aacatctgct ccatggtttc
     3301 ggggtccatt ccgcaaccgc tgtcgctcac ttccagcacc acgtattcac ccggcgccat
     3361 ttcagggaga gggggcggcg ttccctccgc aacgatagcc cgtgaagcga ctatctgtaa
     3421 tatgcccccc gtctcacgca tggcatgaac cgcattcgtg cacaaatttg caaccacctg
     3481 atgcatttgc gcagcatcgg cgagtattcg gcaatcatcc gctgttccct tccagagaat
     3541 ctctatggtg gacgggaatg ccggtccgag cagtttgaga gtatcttcaa cgacctctac
     3601 caatgccagc ggtttcctgt cctgttctcc gcgacgactg aaaaggagta tctgtttcac
     3661 caggtcacgc cccctgtggg ccgctttcaa taccattgtg agccttctgt gttccggcct
     3721 gtcaggactg gtatcttcaa ggaccatttc ggcgaaaccg ataatgcctg cgaggacatt
     3781 gttgaaatca tgggcgatgc cgccggcaag ggtgccgagg gcctccagct tctgggcccg
     3841 gcggagctgc tgctcgaagc tgcgctgttc cgtaagatcg ctgataacga gacagagaca
     3901 ctccacgccc tgaagctgga tggggcacgc cgatacggaa gtcgttatct cgcgtccccc
     3961 actggtgatg aggcgatact ctccccggca gcctgac
//
LOCUS       DAOPOA010000127         3981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_24554_length_3981_cov_4.160978, whole genome shotgun sequence.
ACCESSION   DAOPOA010000127 DAOPOA010000000
VERSION     DAOPOA010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24554_length_3981_cov_4.160978"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PKJ17_03780"
     CDS             complement(<1..898)
                     /locus_tag="PKJ17_03780"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOD75122.1"
                     /translation="MKGVPVIVVIFAVICSILFLPGLSHTQKPEDGDKTLSPYFFVKS
                     DDPKLDQLPLKATSAVANISGVIADVVVTQVYKNEGKKALEAIYVFPASTRAAVYGMK
                     MVIGKRVIEAKIKKRDEARKDYEDAKKQGKSASLLEQQRPNVFQMNVANIMPGDEIRV
                     ELKYTEVLVPTDRIYEFVYPTVVGPRYSNKPADGALPSDKWVQNPYLREGEKPTSTFD
                     MTVTVAGGMPIKELGSTSHKVNTAFNGPANAKVTLDRFEKSGGNKDYILRWRLAGDRI
                     QSGLLLYEGEKENFFLCMLQPPK"
     gene            complement(1164..1703)
                     /locus_tag="PKJ17_03785"
     CDS             complement(1164..1703)
                     /locus_tag="PKJ17_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD75123.1"
                     /translation="MAIRKGKTQKALIRCAGSLLLVALFFLYACNVVYLEKFAQFDAH
                     QGNYEEAVQDLTKAIRYAPRDPVPRAARAYLYQKMGRHDEAIADYTKAIELDPADDEL
                     YLMRGLAYSLKGDQDKAILDYSTAMEIDPHNSDACFNRAQAWETKGNLEQAVKDYQAA
                     ARLGNPRAQGILMARKIAW"
     gene            complement(1901..2782)
                     /locus_tag="PKJ17_03790"
     CDS             complement(1901..2782)
                     /locus_tag="PKJ17_03790"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOD75124.1"
                     /translation="MIADFAKKLETVINFIPPIPVVIVELLKALNDENANMNDIARII
                     AKDPPMSMNVLKVANSAFYRLTNKVATVDHAVRMLGVKELGMICVSCGAYQALKPPAD
                     SETFDINEFWKHSVATGVIAKKICAELSVGDKNTIYFGGLLHDIGKVVLDRFAHEVYK
                     IVIQVTHEECLPMIEAEKRIIGESHDTIGGMIMERWKFPFALADVARYHHAIALCPEE
                     TRPTVAVCALADELARVRYFGFGGDRSGIVLEETEAFRSLHTISPTIANVDVFKFLCD
                     LETVDEEIVEMEKILTT"
     gene            2963..3655
                     /locus_tag="PKJ17_03795"
     CDS             2963..3655
                     /locus_tag="PKJ17_03795"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD75125.1"
                     /translation="METIALIWHNIISLIARDGTKPLAGLSHTKEGRHENRDEDAQYV
                     ALCQGGEPAAFEVLVRRHQKRMFNIAYRMTGNYEDAGDVVQEAFLSAYRTIKHFRGEA
                     MFSTWLYGIVVNHARNRMRQTGSRSYHEPVSLDDPPAGGEGGPAFDPPSRQTPALDVL
                     VRKETQEKVQGCINGLEKEHREIIVLRDIQGFSYEEITGMLGIPDGTVKSRLSRARNA
                     LRESLKKVLGDL"
     gene            3652..>3981
                     /locus_tag="PKJ17_03800"
     CDS             3652..>3981
                     /locus_tag="PKJ17_03800"
                     /inference="COORDINATES: protein motif:HMM:NF021550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2275 domain-containing protein"
                     /protein_id="HOD75126.1"
                     /translation="MRKCADIEDRFTAYLDGYLDTAEREVIEEHLSTCPDCLRKLREL
                     ESARAILAGLDEVDPPPGLTEKIMARIHEEGRVKAGFFRRLFFPLHIKVPVQALATVF
                     VVVLAVYV"
BASE COUNT          808 a         1124 c         1175 g          874 t
ORIGIN      
        1 tcttcggggg ctgcagcatg cagaggaaga aattctcctt ttcgccctca tacagaagaa
       61 gcccggactg tatcctgtcc ccggcgaggc gccagcggag tatgtagtcc ttgttgccgc
      121 ctgacttctc gaatctgtcg agggtcactt tcgcgttcgc gggaccgttg aaagcggtat
      181 tcaccttgtg ggaggtggaa ccaagttcct tgatgggcat accgcccgcg acggtgaccg
      241 tcatgtcgaa ggtgctcgtc ggtttctctc cttcgcgcag ataggggttc tgtacccatt
      301 tgtcggaagg cagcgcgccg tcggcgggtt tgttcgaata acggggaccg accaccgtgg
      361 gatagacgaa ctcgtatatc ctgtccgtcg ggacgaggac ctccgtgtac ttcagctcca
      421 cccgtatctc gtcaccgggc atgatattgg cgacgttcat ctggaagacg ttcggccttt
      481 gctgctcgag aagggacgcg ctcttgccct gctttttcgc gtcctcgtag tccttgcggg
      541 cctcgtcgcg cttcttgatc ttcgcctcga tgacacgttt gccgatgacc atcttcatgc
      601 cgtacacggc ggcccgggtg gaggcgggga aaacatagat cgcctcgagg gccttcttgc
      661 cctcattctt atacacctgg gtcaccacaa cgtccgcgat gacaccggag atgttcgcta
      721 ccgcggatgt ggccttgagc ggcagctggt cgagcttggg atcgtcgctt ttcacgaaga
      781 aataggggga cagcgtcttg tcgccgtcct ccggtttctg cgtgtgcgaa agccccggca
      841 gaaacaggat cgagcagatg acggcaaaga taacaacaat gaccggaaca cctttcatgg
      901 acaacctcct tgagatagct gttttcattc tttttgatga gacacgggag ggcgggaaaa
      961 agttccaaat ggcgcttgcg cgtcggcact tcgtgccaac gggtcggcgc ctgaggcgcc
     1021 taacgagtgg acgggtctgc ccttcgggca aacgggcgga cggggtaaag ataaagaaaa
     1081 acacgtgcac gaagcatttc cgtatgcccg ttaggtgcgt cagcaccgac ccgttggcac
     1141 gaagtgccga cccgtatgcg cacttaccag gctatctttc tcgccatgag gatcccctgc
     1201 gcgcggggat tgccgaggcg ggccgccgcc tgataatcct tcacggcctg ctccaagttc
     1261 cctttggttt cccaggcctg ggccctgttg aaacaggcgt cgctgttgtg cgggtctatc
     1321 tccatagccg tcgagtagtc cagtatcgcc ttgtcctgat cccctttgag ggagtacgca
     1381 aggccccgca tgagatagag ctcgtcatcc gctgggtcga gctcgatggc cttcgtgtaa
     1441 tcggcaatgg cctcatcatg tctgcccatc ttctgataaa gatacgcgcg cgccgcccgg
     1501 ggcaccggat ctctcggcgc gtagcggatg gccttcgtga ggtcctgcac ggcctcctcg
     1561 tagttcccct gatgggcgtc gaactgggca aacttctcga gatagacaac attgcaggca
     1621 taaagaaaaa agagggcgac cagcagaaga gaaccggcac agcggatcag ggccttctgc
     1681 gtctttcctt ttcggatagc catggtgtct tctcctgtct gatggtattt gagcctcatt
     1741 ctatcacgcg gtcgcaggtt tttcaccagc aaaagggaag gggtaggctg gatgggacgt
     1801 atagcgcctg taggtcctat tggtcctggt cgtactatca gctgctccct gtgagggtct
     1861 ttagcctgga acctggaact ttgaaccttt gaaccttttt tcaggttgtc agtatctttt
     1921 ccatctcgac gatctcctca tcgacggttt cgaggtcgca caggaacttg aagacgtcca
     1981 cgttcgcgat ggtgggcgag atggtgtgca gggaccggaa ggcctcggtt tcttcgagaa
     2041 cgatgccgct cctgtcaccg ccgaaaccga agtacctcac ccgcgccagt tcgtcggcga
     2101 gggcacacac ggcgactgtc gggcgcgtct cttcggggca cagggcaatg gcgtgatgat
     2161 accgcgccac gtcggcgagg gcgaagggga atttccacct ctccataatc atcccgccga
     2221 tggtgtcgtg cgactcgccg atgatcctct tctcggcctc gatcatggga agacattctt
     2281 cgtgcgtgac ctggatgacg atcttgtaga cctcatgggc aaagcggtcg aggaccacct
     2341 tgccgatatc gtggaggaga ccgccgaagt atatcgtgtt tttgtcgccg acggacaact
     2401 ccgcgcagat cttcttcgcg atcacacccg tcgcgacgga gtgtttccag aactcgttga
     2461 tgtcgaaggt ctccgagtcc gccggcggct tgagggcctg atacgccccg caggagacac
     2521 agatcattcc cagctccttc acgccaagca tccgcacggc atggtccacg gtggcaacct
     2581 tgttcgtgag gcggtagaat gcggaattgg ccaccttgag aacgttcatg ctcatcggtg
     2641 ggtccttggc gattatccgc gcgatgtcgt tcatgttcgc gttctcatcg ttgagcgcct
     2701 tcaggagttc gacgatgacg accggaatgg gagggatgaa gttgatcacc gtctcgagct
     2761 tcttggcgaa atccgctatc acagatccca gacctccatg gattcccgca ggaccatctc
     2821 tgccggtaat agtatcatcg gataaaggca tcatctctta aggtctcccg gcctggcggc
     2881 ctggcggcaa ccttttttct ttgcaatcga cgtcacggtg ctgtaaaaca tctaaaaatc
     2941 tacctcatcg cgtcgcggcc gaatggaaac tatagccctc atatggcaca acattatatc
     3001 cctgatagcc agggacggaa caaaaccact cgccgggctg tctcatacga aggaagggcg
     3061 gcatgagaac agggacgaag acgctcagta cgtcgcgctg tgccagggag gcgaaccggc
     3121 ggctttcgag gtcctggtga ggagacacca gaagaggatg ttcaacatag cgtatcggat
     3181 gacgggaaat tacgaagacg caggcgacgt cgtgcaggag gcctttctct ccgcatacag
     3241 gacgataaag cattttcgcg gtgaggccat gttctcgacc tggctctacg gcatcgtggt
     3301 aaaccatgcc aggaaccgca tgcgtcagac agggagcagg tcgtaccatg aaccggtgag
     3361 cctcgacgat ccgcctgccg gaggagaagg cggtcccgct ttcgatccgc cctcgcggca
     3421 gacccccgcc ctcgatgtgc tcgtgcggaa ggagacccag gagaaggtgc agggctgcat
     3481 caacggcctc gaaaaggaac accgggagat catcgtcctc agggacatcc aggggttctc
     3541 ctacgaagag atcaccggga tgctcggcat acccgacggc acggtcaagt cccggctttc
     3601 gagagcgcgc aatgccctca gggaaagcct gaagaaagtg ctcggtgacc tatgagaaaa
     3661 tgcgccgaca tagaggatcg tttcaccgcc tatcttgacg gttatcttga cacagcggag
     3721 cgcgaggtga tcgaggaaca tctttccaca tgccccgatt gtcttcgcaa gttgagggag
     3781 cttgaaagtg cgcgggcgat acttgccgga ctcgatgagg tcgatccgcc cccgggactg
     3841 acagaaaaga taatggcacg gatccatgaa gaggggcggg taaaggcggg cttcttccgc
     3901 aggcttttct ttcctctcca cataaaggtc cccgtgcagg cgctggcgac ggtattcgtc
     3961 gtggtcctcg ccgtttacgt t
//
LOCUS       DAOPOA010000128         3910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_25165_length_3910_cov_3.882750, whole genome shotgun sequence.
ACCESSION   DAOPOA010000128 DAOPOA010000000
VERSION     DAOPOA010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25165_length_3910_cov_3.882750"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKJ17_03805"
     CDS             complement(<1..222)
                     /locus_tag="PKJ17_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75127.1"
                     /translation="MTTSGNMTLVGVDVDGTRMTAIVIGTPGKASYLRMAEHVELLFN
                     ESAVSIGKMIEGQMSLNNQLDCTIERLVTG"
     gene            563..1153
                     /locus_tag="PKJ17_03810"
     CDS             563..1153
                     /locus_tag="PKJ17_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75128.1"
                     /translation="MRYHQLIAWIRDEIASTKVVLSEEVVGYEETLEPNPEMYWSEST
                     DLPTTRDRQHVIIKTPIVEVVERTVPDRERRSHAAEALAAFVLSDERYLYLAEELMRR
                     DPLYLPHWERLLLHGRGIRTRKAALRHLGRAPWQIFIRAVGAVAAVFGSLCAVCALIC
                     FYAAIVYCIWPDSKVGVWMASVVKRILAEATRMIYD"
     gene            1163..3367
                     /locus_tag="PKJ17_03815"
     CDS             1163..3367
                     /locus_tag="PKJ17_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOD75129.1"
                     /translation="MKELKVSDKAGKIRRASDDVVREALADLVPGGRTTHRIGGHTDW
                     YHGAAVSPDGSRLITANRDGTARLWDIASGQCLMVFRGHTGEVVRAAFMNHTDRAVTT
                     DDDGTLRLWDCATGACILVLKGHHGRVGGLAVSPDDRYIASSDSFWALRVWSLPEGTC
                     EHSAAGINNVMSILVTRDGNTMLTLDSQHDILRWDFPGLGDPSLVANGDYWDIALSGY
                     GEFVAAGRDGLSCFDVRTGVLKRLYSLEPHKPYKVTTASDETRFVACCSPRETVLVVD
                     RKTGAVEKQFAGPRLVTDMSLSPDGKTLYTVGFDRTCNIWDIGTGECLQAIGSTRSVH
                     HLSLSGNGMYLAACDKGDTVKLWSLTDGSQRAKFPGATSVAFLESARLVVTGHDDGRV
                     RFWDIDSGKEKPGTVLQAYSSAVACVLPLESGAFFATFARHGVPKLWNTEKRNCLRIR
                     NRAGNIDIDTVAIDEDTQRVAMRRQGKNHILVCDLRSGDILATLTDHAYKYGNLSVII
                     TGIRFLPRGRGLVSCGRDGLIILWDLSTFKAARSIHVGNSILSMAVENDGRHVVIGTY
                     AGGVERWDLESGQREICRPPEGSPVAGLALSPDESRLFVSFNDGTVRIIDRQSTALVC
                     SLWNVDDGFFWFTPPDEHAPDGWVWTDREDLLHLVEEGSDGKVLGAVPLADERRGTYI
                     MTRNNAAIVQARLKGLDEYARVVGRYSRALADGQNRGEARPRPMLTGKDNDA"
     gene            3357..>3910
                     /locus_tag="PKJ17_03820"
     CDS             3357..>3910
                     /locus_tag="PKJ17_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75130.1"
                     /translation="MMRKTGEIPPSSSVVSFRPGDLPARAVTGVLKMSAPESRLTHDP
                     GFHKGQIAALAGAFGGTHLLTGTSRGEVRLWDMASGRCVRTFSCGGPTLSGIFTTPGT
                     NRMVTAFLFGTKKVWDLETGDCLATLSDHPGDPVHCNDDHLFCKQNWNVSSLVRGKGP
                     DSKRSEALIYDVNTGKRVRTLRIEG"
BASE COUNT          778 a         1174 c         1196 g          762 t
ORIGIN      
        1 ccccgtcaca agcctctcga tggtgcaatc aagctggttg ttgaggctca tctgcccctc
       61 gatcatcttt cctatcgaca ccgccgactc attgaacagg agctcgacat gctcagccat
      121 acgcaggtac gaggcctttc ccggcgtccc gatcacgatc gccgtcatcc gggtcccgtc
      181 cacatcgacg cccaccagcg tcatgttccc cgatgtcgtc atctcagata tcttcccgcg
      241 caaggtgttc atgaccctca attcgttcga cgcttcactg aaacactcgt tatatatata
      301 cgcacatacc gcctacgaaa gtcaacacaa cctcaaacgc ggcgggccga ctgcggaaag
      361 acgttatctc gcccgcttcg ctccccctca ctgttcgctc tttgtgcaaa atcacccttc
      421 tcttttgcca ttgcctgcac ggtgtattga ctgatatccc atacgtgatg cgatgcagct
      481 atttggagtg aggtccttgg gacgggagat cgggaagttg gggagcggat cgaaggatgc
      541 gcaacaagag gagcggtcgt acatgagata ccatcagctc atcgcctgga tacgggacga
      601 gatcgcgtcc acaaaggtgg ttctcagcga ggaagtggtc ggctatgaag agaccctgga
      661 gcccaacccc gagatgtact ggtcggagag caccgatctt ccgacgacgc gggacaggca
      721 gcatgtcatt atcaagacgc cgatcgtgga ggtcgtggag cggaccgtac cggacaggga
      781 acgtagatcc cacgctgccg aagccctcgc ggcctttgtc ctctccgacg agcggtatct
      841 gtacctggcg gaggaactga tgaggcggga ccccttgtat ctgccgcact gggagcgcct
      901 tttgctgcac ggcagaggca tcaggacgag aaaagccgcc cttcgtcacc ttggcagggc
      961 cccgtggcag atctttataa gggcagtcgg tgcggtggcg gccgtcttcg ggtccctctg
     1021 cgctgtatgc gccctgatct gtttctatgc cgccatcgtt tattgcatct ggccggactc
     1081 gaaagtgggg gtctggatgg cctcggtggt aaagagaatc ctcgcggaag cgacaagaat
     1141 gatatacgat tagaggaagg ccatgaagga actgaaggtt tcggacaagg cggggaagat
     1201 acggcgggca tccgatgacg tcgtgcggga ggcgctggcg gacctcgtcc ccgggggaag
     1261 gaccacccat cgcatagggg gacatacgga ctggtatcac ggagcggcgg tctcccccga
     1321 cgggtcgcgg ctgatcactg ccaaccgcga cggaacggcc cgcctctggg acattgccag
     1381 cggccaatgc ctcatggtgt ttcgggggca caccggcgag gtggtgcggg ctgcttttat
     1441 gaaccacacg gaccgggccg tcacaaccga cgatgacggc accctgcgcc tttgggactg
     1501 cgcgacgggc gcctgcatcc tcgtcctgaa aggtcatcat ggacgtgtcg gcggcctggc
     1561 ggtgagcccc gatgacaggt atatcgcaag cagtgacagc ttctgggcac tcagggtatg
     1621 gtcccttccc gagggtactt gcgaacattc cgcggcaggg atcaacaatg tcatgagtat
     1681 ccttgtgacc cgggacggca acacgatgct cacgctggac tcacagcacg atatccttcg
     1741 atgggacttc cccggccttg gtgatccctc tcttgttgcg aacggtgatt actgggacat
     1801 cgctctttcg gggtatggag agtttgtggc cgccggaagg gacggccttt cctgcttcga
     1861 tgttcgcacc ggcgtcctga agaggctcta ttccctggaa ccgcacaaac cctacaaggt
     1921 gaccaccgct tccgacgaaa cacggttcgt ggcctgctgc agccccaggg agactgtcct
     1981 cgtggtggac cgcaaaacgg gcgctgttga gaaacagttt gccggtccga ggttggtgac
     2041 cgacatgagc ctctcgccgg atggaaaaac cctctatacg gtgggctttg accggacatg
     2101 caacatctgg gatatcggca cgggcgagtg cctgcaagcc atcggttcca cccggagcgt
     2161 gcaccatctc tccctttccg gcaacggcat gtatctcgcc gcctgtgaca aaggcgacac
     2221 ggtcaagctc tggagcctca cggatggttc ccagcgggcg aagttcccgg gggcaacgtc
     2281 cgtcgccttt ctcgaaagcg cccgtcttgt tgtcaccggg catgatgacg gccgggttcg
     2341 attctgggac atcgatagcg gcaaagagaa gcccgggacg gtcttgcagg cctactcatc
     2401 cgcggtcgcc tgcgtcctgc cgctggaatc aggcgccttt ttcgcaacct tcgcccggca
     2461 cggagtgccg aagctctgga acacggaaaa acgcaattgt ctcaggatcc gtaacagggc
     2521 cggaaatatc gacatcgaca ccgtcgcgat agatgaagac acacaacgcg tggcgatgcg
     2581 tcgccagggg aagaaccata tacttgtctg cgatcttcgg tccggcgaca ttcttgccac
     2641 gctgacggac cacgcataca agtacggcaa cctctccgtc atcatcacgg gcattcgttt
     2701 tctcccccgg ggaaggggtc ttgtttcctg cggccgtgac ggccttatca tcctctggga
     2761 cctttccacg ttcaaggccg cgcgttctat ccatgtaggt aattcgatcc tctccatggc
     2821 cgtcgagaac gacgggcgtc atgtggtcat cggcacctat gcgggagggg tggaacgatg
     2881 ggacctcgaa agcgggcaac gcgagatctg ccggcctcct gaagggagtc cggtggcggg
     2941 gctcgcgctc tctcccgacg aaagccggct cttcgtgtcc ttcaacgacg ggaccgtgag
     3001 gatcatagac agacagagca cggccctcgt ctgctccctc tggaacgtgg atgacggctt
     3061 cttctggttc acaccgcccg acgagcacgc cccggacggc tgggtgtgga ctgacaggga
     3121 agacctcctc catctcgtcg aggagggcag cgatggcaag gtccttggtg ccgtacccct
     3181 ggctgacgaa agacgaggaa cctatatcat gacccgcaac aatgcggcaa tcgtgcaggc
     3241 gcgccttaag ggcctcgatg aatacgcgcg cgtcgtgggt cgctatagcc gtgcccttgc
     3301 ggacgggcag aacaggggcg aggcccgccc gcgaccgatg ctgaccggga aagataatga
     3361 tgcgtaagac aggggaaata ccgccgtctt cctcggtcgt gtcctttcgc cccggggacc
     3421 ttccggccag ggcggtgacc ggggtcctca agatgtccgc gccggagagc cgtctgaccc
     3481 acgatcccgg gttccacaaa ggacagatcg ccgcgcttgc gggggcattt ggcggcaccc
     3541 acctgctcac gggaacctcg aggggtgagg ttcgcctgtg ggacatggcc tccggaagat
     3601 gcgtacggac gttctcctgc ggaggcccca ccctttcggg catcttcacg acacccggga
     3661 cgaaccgcat ggtaacggcc tttctcttcg gtaccaagaa ggtctgggac ctcgaaacag
     3721 gcgattgcct ggcgaccctg tccgaccatc ccggcgaccc cgtgcactgc aacgacgatc
     3781 atctcttctg caagcagaac tggaatgtct cgagtctcgt gaggggaaag ggtccggact
     3841 cgaaaaggtc cgaggccctg atatacgacg tgaacacggg gaagagggtc cggacgttac
     3901 ggatcgaggg
//
LOCUS       DAOPOA010000129         3903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_25223_length_3903_cov_3.715177, whole genome shotgun sequence.
ACCESSION   DAOPOA010000129 DAOPOA010000000
VERSION     DAOPOA010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25223_length_3903_cov_3.715177"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PKJ17_03825"
     CDS             <1..916
                     /locus_tag="PKJ17_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOD75131.1"
                     /translation="SLLQIVMQYPYLRKFGIRYHFSLDLGHPAVATAKRLFTGALIAT
                     SIVPINSFVGRIIASYLPHGEVASLSYAFRIFILPFSLFAVPVYTVMFSKLSKLFHDN
                     DMGDFRAHIDSSVVLICVTLIPATILLCATGDLCVKLLYERGAFTATDTAMTNRALFG
                     YSVGLVFYALSISFVRIFNAMHDMKTPALIGITSIALNAIIASVLMVPYRNLGISLAT
                     SIVSLYNFLFLYVILRHRTGYRLARPAVRDIIKSLLAGILLILLIVLTRRLLPGSLLP
                     PLFASALLTVAVYGIVFKNYYRRLLRRR"
     gene            921..1772
                     /locus_tag="PKJ17_03830"
     CDS             921..1772
                     /locus_tag="PKJ17_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75132.1"
                     /translation="MPVLSRNELTPENVLKHIGKIHDENYGNHGIPLISRVFKDVVDL
                     FNGEWPGYHRCDTQYHDLYHTMQTVPPFVEIISGWNKSGASPRISESFFSYGTIAVLL
                     HDTGYIKELGDDEGTGAKYTLTHVQRSINFANVYLRQVGLPVTEIRHILNIIRCTGVT
                     IDLNVRFRNEQERVVGCALGTADLLGQMSSSDYIDTLPILFDEFAESYGFEGVRKLHK
                     KGSTLYADADELIRSTPTFYEKVALKRFEMMGSMEKYIPRHYGRKRNPYVAAVEKNIR
                     TIRKKWK"
     gene            2031..>3903
                     /locus_tag="PKJ17_03835"
     CDS             2031..>3903
                     /locus_tag="PKJ17_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOD75133.1"
                     /translation="MTKTIREFHVSRKARDTYHFSDALFALSGNVIFTNFLAARTFAQ
                     KMNDKRDLILYPESAIRAGALIAMGLIDEILHYVVSVYAESVRRDVMAGALSHLEATL
                     GPAELERTIRQFSDEFPPLAVYQRKTTLDEYLSGQTGETPNRWIVLEEMLLLWLANMN
                     PAFSPFTELFDDSSLRRETSFLKTIAELRVFFDSQPPFGPEGQNLIDLLRSPAVAVPH
                     SLSGQLEYIMERWGYLLGSRFISRMLKSLDLFKEEEKMPFLGPGKAQVYRYTGLEFEP
                     EQFSADKDWMPRLILIAKNVYVWLDQLSRTHGRSIHRLDQIPDEELDILRKRGFTGIW
                     LIGVWERSAASQRIKQMCGNPEAVPSAYSLHDYIIARDLGGEEAFLQLKDRATRRGIR
                     LASDMVPNHVGIYSRWVIEHPDWFVSIGHNPFPWYSFGGPNLSHDDRVSIQIEDHYYD
                     RTDAAVVFKRRDNWTGDEKFVYHGNDGTSMPWNDTAQLNYLNPEVREAMIRTIIHVAR
                     KFPVIRFDAAMTLTKKHYQRLWFPEPGTGGAIPTRSEHSLTYDDFSKAMPAEFWREVV
                     DRVAAEAPDTLLLAEAFWLLEGYFVRTLGMHRVYNSAFMNMLRDEENSKYRLVMKNTL
                     "
BASE COUNT          833 a         1247 c          994 g          829 t
ORIGIN      
        1 gtccctgctg cagatcgtca tgcagtatcc ttatctcagg aaattcggca tccgctacca
       61 cttctccctt gatctcggcc acccagccgt tgccacggcc aaacgtcttt tcaccggcgc
      121 gctcatcgcg acgtccatcg tgcccatcaa cagcttcgtc gggaggatca tagcctccta
      181 cctcccccac ggggaggtgg cgtccctctc ctacgccttc aggatattca tccttccctt
      241 cagcctcttc gcggttcccg tgtacacggt gatgttctca aagctgtcaa agctcttcca
      301 cgacaacgac atgggcgatt tcagggccca catagacagc tccgtcgttc tcatctgcgt
      361 gaccctcatc ccggcgacaa ttttgctgtg cgccacgggc gacctgtgcg tgaagctcct
      421 ctacgaacgc ggagcattta ccgcaacaga cacggcaatg acgaaccggg ccctcttcgg
      481 ctacagcgtg gggctcgtct tctacgccct ttccatctcc ttcgtgcgaa tattcaacgc
      541 catgcacgat atgaaaacgc cggcccttat cggcatcacc tccatcgcgc tcaacgccat
      601 catcgcctcc gtcctcatgg tcccttacag gaacctcggg atctccctgg ccacgtccat
      661 cgtgtccctc tacaacttcc tcttcctcta tgtcatcttg aggcacagga cggggtacag
      721 gctggcccgc cccgccgtgc gggacatcat caagtccctt cttgccggta tactcctcat
      781 ccttctcatc gtcctcacac gccggctcct gccgggaagc cttcttcccc cgctttttgc
      841 aagcgctttg ttgaccgtcg ccgtttatgg tatagtgttt aaaaattatt accgaagact
      901 cttgcgcagg agatgatgcc atgcccgtat tgtcaaggaa cgagctgacg cctgagaacg
      961 tcctgaagca catcgggaag atccatgacg agaattacgg caatcatggc atacctctga
     1021 tctcgcgggt cttcaaggac gttgtcgacc tcttcaacgg cgaatggccc ggctatcacc
     1081 ggtgcgacac gcaataccat gacctctacc acaccatgca gaccgtgccg cctttcgtgg
     1141 agatcatcag cggctggaac aagagcggag cctccccgag gatctcggag agtttctttt
     1201 cttacggtac catcgcggtc cttcttcacg atacggggta catcaaggag cttggcgacg
     1261 atgaaggcac gggtgccaaa tataccctca cccatgtcca gcgcagcatc aatttcgcca
     1321 acgtctacct ccgtcaggta ggcctgcccg tcaccgaaat acgccacatc ctcaacatca
     1381 tcaggtgcac cggcgtcacc atcgacctca atgtccgctt ccgcaacgag caggaacgcg
     1441 tggtcggctg cgccctgggc actgccgatc tgttgggtca gatgtcatct tccgattaca
     1501 tcgacaccct ccctatcctc ttcgacgaat tcgccgaatc ctacggtttc gaaggggtca
     1561 ggaagcttca caagaagggc tccaccctgt acgccgatgc cgacgaactc atcaggtcga
     1621 cgcccacatt ctatgaaaaa gtggccctca agcgttttga aatgatgggt tccatggaaa
     1681 agtacatccc ccgccactat ggccgaaaaa ggaacccata cgtggccgct gtggaaaaga
     1741 acatccgaac catcaggaag aaatggaaat aaacagagtt ccgggtcgcg ggttgcgtgt
     1801 tccgggttga ggcaaagcct caggaagtga aaacgctttc aggtctcacc gaaaacttcc
     1861 cctctcaagg ttccaatctt cccggacaat cggtccctcc ttggtttttg acccgaaacg
     1921 cgcaacccgg aacccgcaac tgtcttttgc ctgaaacgtg aaacctcaat tgacggaatc
     1981 cttttactgt gttagcattc cttctacgta agggcaaatg gtgtagacct atgacaaaga
     2041 ccatcagaga gttccacgta tcacggaagg ccagggacac ctatcatttc agcgacgccc
     2101 tcttcgcctt gagcggcaac gttatattta ccaactttct cgcggcccgc accttcgcgc
     2161 agaagatgaa cgacaaacgg gaccttatcc tctaccccga gagcgcgata cgcgcgggtg
     2221 cgctcatcgc catgggcctc atcgacgaga tccttcatta cgtggtcagc gtgtacgcgg
     2281 aatccgttcg gagggacgtc atggccggtg ccctctccca ccttgaggcc actcttggcc
     2341 ccgcggaact ggaaaggacg atacgccagt tcagtgacga atttccgccc cttgccgtct
     2401 accagaggaa gacgacgctc gatgagtacc tcagcggcca gacgggtgaa acccccaacc
     2461 ggtggatagt cctcgaagag atgctccttc tctggctcgc gaacatgaac cccgccttct
     2521 cccccttcac cgaacttttc gacgactctt ccttgaggag agagacctcc tttctcaaga
     2581 ccatcgccga gttgagggtc tttttcgact cccagccccc cttcggcccg gagggccaga
     2641 acctgataga cctgctccgc agcccggccg tcgccgtacc ccattcgttg tcggggcagc
     2701 tggaatacat catggaacgg tggggatacc tcctcggatc caggttcatt tcccgcatgc
     2761 tcaagagcct cgaccttttc aaggaagagg agaagatgcc ttttctcgga cccggcaagg
     2821 cccaggtgta tcgctatacc ggtctcgagt tcgaaccgga acagttcagc gccgacaaag
     2881 actggatgcc gcgtcttatc ctcattgcca agaacgtcta cgtgtggctc gaccagttgt
     2941 cacgaaccca tggcaggtcg atacacaggc tcgaccagat acccgacgag gagctcgaca
     3001 tcctcaggaa acggggtttt accggaatat ggctcatcgg ggtctgggaa cgcagcgccg
     3061 cctcgcagcg catcaaacag atgtgcggta acccggaggc ggtcccttcg gcctattccc
     3121 tgcacgatta catcatagcc cgggaccttg gcggcgagga ggccttcctt cagctcaagg
     3181 accgcgccac gcgaagaggc atccgcctgg ccagcgacat ggtgccgaac catgtcggca
     3241 tctattcgcg ctgggtcatc gagcaccccg actggttcgt ttccattgga cacaaccctt
     3301 ttccctggta ttccttcgga ggacccaacc tctcccacga tgaccgcgtt tccatccaga
     3361 tagaagacca ttactacgac cgcaccgatg cggcggtggt cttcaagcgg cgtgacaact
     3421 ggacgggcga cgaaaagttc gtctatcacg ggaatgatgg cacgagcatg ccctggaacg
     3481 acaccgcgca gttgaactat ctcaatcccg aggtgcgcga ggcgatgatc cgcaccatca
     3541 tccacgtggc ccggaagttt ccggtcatcc gcttcgatgc cgccatgacg ctcacgaaga
     3601 agcattacca gaggctctgg tttcccgaac cgggtacggg cggggcaata ccgactcgaa
     3661 gtgagcacag cctgacctat gacgacttct cgaaggcgat gcccgcagag ttctggcgcg
     3721 aggtcgtgga ccgtgtcgcc gcggaggcgc cggacacgct cctgctggcc gaggcgttct
     3781 ggctgctgga ggggtacttc gtccggacgc tcgggatgca tcgtgtttac aacagcgcct
     3841 ttatgaacat gctgcgcgac gaggagaact ccaagtaccg tctggtcatg aagaacaccc
     3901 tgg
//
LOCUS       DAOPOA010000130         3882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_25399_length_3882_cov_2.818918, whole genome shotgun sequence.
ACCESSION   DAOPOA010000130 DAOPOA010000000
VERSION     DAOPOA010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25399_length_3882_cov_2.818918"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKJ17_03840"
     CDS             complement(<1..238)
                     /locus_tag="PKJ17_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75134.1"
                     /translation="MKSACLAIAVLLVLCFFFAGCGEKEVPRTVKVYPLDNMEGVLTQ
                     SNVAFDRAVSADGRGSLRIDAPSPMTVRLYEVKDI"
     gene            432..566
                     /locus_tag="PKJ17_03845"
     CDS             432..566
                     /locus_tag="PKJ17_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75135.1"
                     /translation="MMKQARTILSSLGKKISVLRRGSKIVLVLCAWLLVISMLLAFGL
                     "
     gene            704..1516
                     /locus_tag="PKJ17_03850"
     CDS             704..1516
                     /locus_tag="PKJ17_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75136.1"
                     /translation="MMGGIEESCYAILGLPVGAPQEELEEAFRRLKRESEGDWDRLKE
                     ITWAYETLAGSRAGAGKEGNGGTGLTGPGDENETTLIGLLFPPQVKVNPFYFWGRCLV
                     FMLLLIAGVRLMVNPPSSELAGHSFFHNISLPFHEAGHIVFRILGDFMGVLGGSLMQV
                     LIPVICLAAFLKRGDAFAASFALWWTGQNFIDMAPYINDARAQQLMLLGGVTGQDVPG
                     YHDWNNILGRLGLLNLDHGLANASHIVGSLLMTAAFIWAAIILWMQWKNLDR"
     gene            complement(1662..2219)
                     /locus_tag="PKJ17_03855"
     CDS             complement(1662..2219)
                     /locus_tag="PKJ17_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HOD75137.1"
                     /translation="MEEKLANPAPLGLMGFGMTTVLLNIHNAGFFPVGSMILAMGAFY
                     GGLAQIIAGILEYKKGNTFGVTAFTSYGLFWWTLVFILVFKGLPGNPEAFMGWYLFMW
                     GLFTFFMWFGTWGKNRAIQFVFLSLTILFWLLAIRDWSGSAAIGTLAGWEGIICGLSA
                     VYLAMAEVLNEVHGRTVLPIGEIKK"
     gene            complement(2398..3033)
                     /locus_tag="PKJ17_03860"
     CDS             complement(2398..3033)
                     /locus_tag="PKJ17_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOD75138.1"
                     /translation="MLENLAIIGTIAFAHLLGAMSPGPDFAMSVRNSLAYSRRTGIFT
                     AVGFGLGVAVHVGYCIAGIAVVIAKSIVVFSTIKYLGAAYLLYIGIRSVFSRENHVEP
                     DVVVKRDDISPFAAVRMGFFTNILNPKATLFFLGLFTVVISPAAPPPRHILVIAGLVM
                     VVDTMLWFSFVAVFLNERHVRSFFERFQGYLNRAFGGLLILLSITIAFSRR"
     gene            complement(3224..3499)
                     /locus_tag="PKJ17_03865"
     CDS             complement(3224..3499)
                     /locus_tag="PKJ17_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOD75139.1"
                     /translation="MILTAEKILPGGDCLARIDGKAVFLSGALPGEVVDAARRGGTPV
                     ESAEEISHTALDRGSTDNITVIVLNLRKYTSCLKNRKMVIRKVYDRA"
BASE COUNT          917 a         1065 c         1061 g          839 t
ORIGIN      
        1 cgatgtcctt gacctcatag agccggaccg tcatgggcga cggggcatcg atcctcaggg
       61 aacccctgcc gtccgccgac acagcgcgat cgaaagcgac attcgactgc gtcaggaccc
      121 cctccatgtt gtcgagggga tagaccttga cagtccgggg cacttccttc tcgccgcagc
      181 ccgcaaaaaa gaagcagaga accagaagga ctgctatcgc aagacatgcg cttttcatgg
      241 gatggtctcc ttccgcagca tttcaaagga ctcctgacgt cgtcctatag cattatccta
      301 tcacaacagg ccgccagggt gaagagatgt tcgcggcaaa gatccgggaa agaggcgcag
      361 gagcggctgg tatttaataa taccgggaac tttccgataa cctgaaatag catcagcacc
      421 gaggtgaaga catgatgaaa caagctcgta cgatactctc ctcccttggg aagaagatct
      481 ctgtcctcag gaggggctcc aagatcgtac ttgtgctgtg cgcgtggctg ctcgtgatca
      541 gcatgctgct ggcgttcgga ctgtagaggc ggtcccaggt tccaaggttt catgtttcag
      601 gccagaacga gaaggcgtcc ccttccttga gctgaagcat gtaacttgaa acctgaaact
      661 tgaaaccctg tttgctccct gtcggttgac atggtaggtt agaatgatgg gtggtatcga
      721 ggagagctgt tatgccattc tcggccttcc cgtgggggca ccacaggaag agctggagga
      781 ggctttcaga cgcctgaaga gggaatcgga gggcgactgg gacaggctca aggagatcac
      841 ctgggcctac gagacgctgg cgggatcccg ggccggcgcg gggaaagaag gaaacggggg
      901 aacgggcctc accggaccgg gtgatgaaaa cgaaaccact ctcatcggtc ttctcttccc
      961 tccccaggtg aaggtaaacc ccttctattt ttggggaaga tgcctggtat tcatgcttct
     1021 tctcatagcc ggcgtgaggc tcatggtcaa tccgccgtca agcgagctgg cgggccattc
     1081 gttcttccac aatatcagtc tccccttcca cgaggccgga cacattgtct tccgcatcct
     1141 cggcgacttc atgggcgtgc tgggaggaag cctcatgcag gtgctcatac ccgtcatctg
     1201 tctcgccgcc ttcctgaaac ggggcgacgc ctttgcggcg tccttcgccc tgtggtggac
     1261 gggacagaat ttcatcgata tggcccccta catcaacgac gcccgagctc agcagctgat
     1321 gctcctcgga ggcgtgacgg gccaggatgt ccccggatac cacgactgga acaacatcct
     1381 cggccgcctc ggcctcctca acctcgacca cggcctggcc aacgcatccc acatcgtcgg
     1441 ctccctcctg atgacagccg ccttcatctg ggcagccatc atactctgga tgcagtggaa
     1501 gaatttggac agataaaagc ggcttgagta gcttgaatcg cttgaaaagc ttgagcgtga
     1561 aagatcaaga aacggcttga ggggttcgaa cgatcgagag ttggcctctt tgtcttctaa
     1621 ctctcaaacg attcaagccc ctcaagcgat tcaatccgtc tttacttctt tatctccccg
     1681 atgggaagta cagtccttcc gtggacttca ttgaggacct ctgccatggc caggtagacg
     1741 gcggagaggc cgcagatgat gccttcccat ccggcaaggg tgccgatggc ggcgctgccg
     1801 ctccagtcac ggatggcaag aagccagaag agaatggtga ggctcaggaa cacgaactgg
     1861 atggccctgt tcttgcccca tgtgccgaac cacatgaaga aggtgaagag gccccacatg
     1921 aaaagatacc atcccatgaa agcctcggga tttccgggaa ggcccttgaa aacgagaatg
     1981 aacacaaggg tccaccagaa gagaccgtac gaggtgaacg ccgtcacgcc gaaggtgttc
     2041 cctttcttgt attccaggat gccggcaatg atctgggcaa gaccgccgta aaaggctccc
     2101 atcgcgagta tcatcgatcc cacggggaaa aatcccgcgt tgtggatgtt cagaagcacg
     2161 gtggtcatac cgaatcccat gaggccaagt ggtgcggggt tagcgagctt ttcttccata
     2221 gtgaactcct ccttgtgaat ggttagtaga ttattccacc cctgggataa ccgtgatgct
     2281 tccctgtgcg ccttgtgcgt ttcgaaaacc cttgaacccc gaaaaaggtc cgcgtctact
     2341 atagcatgtc gaaggacctc tgtcatcacc tttgctccgg ttcttccgcc gttgcggcta
     2401 acggcgtgag aaggctatcg tgatgctcaa gaggatgaga aggcccccga aggccctgtt
     2461 gagataaccc tgaaaccttt cgaagaagga cctcacatgc ctctcgttga gaaagaccgc
     2521 cacgaagctg aaccacagca tggtgtccac gaccatgaca aggcccgcga tgacaagaat
     2581 atgacggggc gggggcgcgg cgggggatat gacgacggtg aagaggccaa ggaagaagag
     2641 cgtggccttt gggttcagga tgttcgtgaa aaatcccatt cggaccgctg cgaagggcga
     2701 gatgtcgtcc cttttgacaa ctacgtctgg ctccacgtgg ttctcccggc taaagaccga
     2761 gcgtatccct atgtacagaa gatatgccgc ccccaggtac tttatcgtgc tgaagacaac
     2821 gatggatttg gcgatcacga cggctatccc ggctatgcag taaccgacat gaacggcgac
     2881 tcccagtccg aatcccacgg ccgtgaatat ccccgtcctt cgggagtagg ccagggaatt
     2941 gcgcaccgac atggcaaaat ccggcccagg gctcatggcg ccgaggaggt gggcgaatgc
     3001 gatcgtcccg attatggcaa ggttttcgag catatcaccc tgttgaagat aatgaccaat
     3061 gatcaaattc caatgaccaa acaataacca atataccaat aaccacagga aaaaacgaga
     3121 acaggtggac gccgcccggg ttactcgaac acgggccttt cacgtttggt cattatcttc
     3181 ttcattggtc attgggattt gatcattggt cattgtcatt ttttcacgct ctgtcatata
     3241 ccttccggat aaccatcttc cggtttttca ggcaggatgt gtatttcctg aggttcagca
     3301 cgatcacggt gatattgtcc gtactgcccc tgtcgagagc cgtgtgcgat atctcttccg
     3361 cgctctccac gggggtcccg ccccgccttg ccgcgtcgac gacctcgccg ggaagcgccc
     3421 cggacaggaa taccgccttt ccgtctattc gggcaaggca gtctccaccg ggcaggatct
     3481 tttcggcagt aagtatcatt attgtatgaa tatagcaaat aagtcggatt aaatcccgta
     3541 tttatatggt caaagggcgt tctcccgaga cttgcttgac aactacgttg acacgccccg
     3601 cgtttctgta gaaaatggat aaaagatgtt aatacgttac actaaggatg aacgcggcaa
     3661 aacggttcgc caggcattgg tctacacaag acgcgagcac ggaatcgatt cgcgcttaat
     3721 cgacccaaac gcgttacggg taatatcaca cctgaaaagc tttggctttg acgcttatat
     3781 cgtcggggga gcggtacgcg acctcattat cggaaaaaat cccaaggatt tcgatatcgt
     3841 caccaacgcc gaacctgccc ggatcaagcg actttttagg aa
//
LOCUS       DAOPOA010000131         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_25990_length_3817_cov_3.947103, whole genome shotgun sequence.
ACCESSION   DAOPOA010000131 DAOPOA010000000
VERSION     DAOPOA010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25990_length_3817_cov_3.947103"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKJ17_03870"
     CDS             <1..430
                     /locus_tag="PKJ17_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD75140.1"
                     /translation="SALYPDLTLAGVTGLFMPVIIPRLAYCEYHCTLCGQVCPTGAIP
                     RLPLGQKQKSIIGTAVIDKNICLPYAKKLNCIVCEEHCPIPEKAIRFETMDDIDRQGR
                     KVTLKRPYIVDNLCNGCGICENKCPLEGRSAVEVFAGKKG"
     gene            complement(689..3736)
                     /locus_tag="PKJ17_03875"
     CDS             complement(689..3736)
                     /locus_tag="PKJ17_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD75141.1"
                     /translation="MNDYDPEKEQVVSSGGDDGVRVFFDTPPDAAFIMDSQGTLITLN
                     ENLARRFNHDVDEMVGSNIFDYFSPDVARSRKALMERIITGRRPLSCTDFRDGVWLES
                     CYYPVLDRSGSVAKIAVFSRDVTAVRRAEEALRESEERYRTAIENSNDGVAILKGNIH
                     LYVNGKFVEIFGYDSPADIIGKPQSLTVHPDDYRRVVDFTQRRQRGEIIPERYEFKGV
                     KRNGDLVYIEVSATGTTYRGENVALVYMRDVTARRTSEEELWLARFSIEHASESIFWI
                     RPDGRFVYVNEAACNKLGYRKEELLALRVVDVDPDHTKRELSAIGRCMAEEGSITFRS
                     HHQAKNGRTFPVEVIANHVAYSGEDFIFCFARDVSDKELAEERLNAERQKFQILIEHA
                     PFGMAMFDREDRYLYVNPKFTEIFGYTLAEIPDRASWFERAYPDGAVRESVTDAWHHD
                     DGTKDVGEKMPRTFPVVCKDGATKLINFITVRLENGDYIVSFEDITERRLAEQALANE
                     KERLAVTLRSIGDGVIATDSDGRIVLMNAVAEELTGWKQEEATGTDLVDVFHIINERT
                     RARCESPVDKVLRMGTVVGLANHTALISKDGRELVIADSGAPIWDRDGNIAGVVLVFR
                     DVTEKKRMDEELQKMSKLESVGILAGGIAHDFNNILAAILGNVSLARMYAEPADERVL
                     KRLEDAEQAVLRAKDLTLQLLTFSKGGSPILKTTSIENVVRETASFALTGSGVRCDFM
                     FAEDLFPVDIDEGQMSQVINNLVINSQQAMSGGGVITIEAVNLAAPDEMVEHGVLLQP
                     GSYVRISVKDRGTGIAPEHVHKVFDPYFTTKDKGSGLGLATSYSIIKNHDGHITLDSE
                     PGSGTTFFIYLKASRRRAPSVVRSVRDNRITTVRARILHMDDEAMILKTTKEMLEGLG
                     YDVACAADGREAIARFREAAAAGTPFDVVLLDITVPGGLGGKGTMRELVAIDPGVRVI
                     VSSGYSNDPVLADHRKHGFKGVLAKPCLMSELDEIIRRVLEEG"
BASE COUNT          757 a         1192 c         1024 g          844 t
ORIGIN      
        1 cagcgccctc taccccgatc tcacgctggc cggtgtcacc ggcctcttca tgcccgtcat
       61 cattccccgg ctggcctatt gcgagtatca ttgcacgctc tgcggacagg tctgccccac
      121 cggcgccata ccccgtctcc ctcttggcca gaagcaaaaa agcatcatcg gcaccgccgt
      181 catagacaag aacatctgcc ttccctacgc gaaaaagctc aactgcatcg tctgtgaaga
      241 acactgcccc atcccggaaa aggcgatccg cttcgaaacg atggacgaca tcgaccgcca
      301 gggcagaaag gtcaccctca agcggcccta catcgtcgat aacctctgca atggctgcgg
      361 catatgcgag aacaaatgcc cgcttgaagg ccgctcggcc gtggaggtct tcgcgggaaa
      421 aaaaggctga actcccgaac caaaggggtt tgaccgttag gggccgctcg gccgtagagg
      481 ttcgtgcgac aaagaaaggt cgaatcccga acaggaaggg gtttgaccgt tggacgcgtt
      541 agcgccgttg gaacgataga aacaagaaag tcattgcttc tgcatgatgg ggtggttgag
      601 gtttctccaa tgcccaagag gctcaagtta ttgctaaccg tctctcggtt ccaacggctc
      661 taacggtgct aacgattcca acggtttctc agccttcttc cagcacccgc cttatgatct
      721 cgtccagttc cgacatgagg cagggtttgg cgaggacgcc tttgaaaccg tgcttccgat
      781 ggtctgccag aacggggtcg ttggagtagc cgctggagac tatgaccctg accccggggt
      841 ctatggcgac cagttccctc atcgtgccct ttccccccaa ccctccgggg acggtgatgt
      901 cgagaaggac gacatcaaag ggagtacccg cggcggcggc ttcacggaac ctggcaatcg
      961 cctcccggcc atccgccgcg caggccacat cgtaaccgag gccctccagc atctccttcg
     1021 tggtcttgag tatcatcgcc tcgtcgtcca tgtggaggat ccttgctctg accgtggtga
     1081 tcctgttgtc acggacactc ctcacgacgg aaggggcgcg ccgccgggac gccttaaggt
     1141 agataaagaa ggtcgttccc gaaccgggtt cggaatcaag cgtgatatgg ccgtcgtgat
     1201 tcttgatgat agaataggac gtcgcgagtc cgagaccgct gcccttgtcc ttcgtcgtaa
     1261 agtagggatc gaataccttg tgcacgtgtt ccggggctat ccccgtaccg cggtctttca
     1321 ccgatatccg gacatagctg ccgggctgaa gtaaaacacc gtgctccacc atttcatcgg
     1381 gggcagcaag gttcaccgcc tcgatcgtga tcaccccgcc accggacatg gcctgttggg
     1441 agttgatgac gagattattt atgacctggc tcatttgacc ctcgtctata tccacgggga
     1501 aaaggtcttc ggcaaacatg aagtcgcacc tgaccccgga gcccgtgaga gcgaaactgg
     1561 cggtttccct gaccacattt tcgattgagg tcgtcttcag gatgggagaa ccgcccttcg
     1621 agaaggtcag aagctggagg gtcaggtcct tggccctcaa taccgcctgt tcggcgtcct
     1681 cgagacgttt cagcaccctc tcatctgcag gttccgcgta cattcttgcc agagacacgt
     1741 tccccagtat agccgcgagg atgttgttga aatcgtgagc tattcccccc gccaggatac
     1801 ccactgactc aagcttgctc atcttctgca gttcctcgtc cattctcttc ttttccgtca
     1861 cgtcacgaaa gacaagcacc acaccggcga tattcccgtc cctgtcccat atcggggcgc
     1921 cgctgtcggc tatgacaagt tcccgcccgt ccttcgagat gagggccgtg tggtttgcga
     1981 ggcctacgac cgtccccatc cgaagtacct tgtcgacggg actttcgcat cgtgccctag
     2041 tcctctcgtt gatgatgtgg aaaacatcga caagatccgt tcctgtggcc tcctcctgtt
     2101 tccatcccgt cagttcttca gcaacggcat tcataagaac gatccttccg tcggagtcgg
     2161 tggcgatcac gccatctccg atggaacgca gcgtgaccgc gagtcgttcc ttttcattcg
     2221 ccagtgcctg ttcggcaaga cggcgctccg ttatgtcctc gaaggagact atgtagtcgc
     2281 cgttctcgag gcggacggtg atgaaattga tgagctttgt cgccccgtcc ttgcacacca
     2341 ccggaaaggt cctcggcatt ttctccccga catccttggt gccgtcatca tgatgccagg
     2401 cgtcggtcac gctctccctc acggccccgt cgggataggc acgctcgaac catgaggccc
     2461 ggtccggtat ctcggcaagc gtgtacccga aaatctccgt gaactttggg ttcacataga
     2521 gatatctgtc ctccctgtcg aacattgcca tgccgaacgg ggcgtgttct atgaggatct
     2581 ggaacttctg ccgctccgcg ttcaatcttt cctcggccag ttccttgtcg gagacgtccc
     2641 gggcgaaaca aaagatgaaa tcctccccgc tgtaagcaac atgattcgcg atgacctcga
     2701 cggggaaggt gcggccattc ttggcctggt gatgggacct gaaagtgatg gagccctcct
     2761 ccgccatgca cctgcctatg gcggagagtt ccctcttcgt gtggtcggga tcgacatcta
     2821 cgacccgcaa agcgagaagc tcctccttcc tgtacccaag cttgttgcac gcggcctcgt
     2881 tcacgtacac gaacctgccg tcgggtctga tccagaagat cgattccgag gcgtgttcga
     2941 tggagaaccg cgcgagccag agctcttcct cgctcgtccg cctggccgtc acgtccctca
     3001 tgtacaccag ggcgacgttc tcccccctgt aggtggtccc cgtcgcggaa acctctatat
     3061 agacgaggtc gccgttgcgc ttgaccccct tgaactcata ccgctccggt attatctcgc
     3121 cgcgctgcct gcgctgtgtg aaatcgacca ccctccggta gtcgtcggga tggacggtga
     3181 gggattgcgg tttcccgatg atgtcggcgg ggctgtcata gccgaagatc tcgacgaact
     3241 tcccgttcac gtaaagatgg atgttccctt tcagtattgc cacgccgtcg ttggaatttt
     3301 cgatggccgt ccggtaccgc tcctccgact ccctcagggc ctcctcggcc cggcggacgg
     3361 ccgtcacatc gcgactgaac accgcgatct tcgccaccga ccctgaccgg tcgagaacgg
     3421 gatagtaaca gctctcgagc cagacaccat cgcggaaatc ggtgcaggaa aggggccttc
     3481 gccccgtgat tatcctctcc atcagggcct tcctgctacg ggccacatcg ggtgagaaat
     3541 aatcgaatat gttgctgccc accatttcgt ccacatcgtg gttgaaacgg cgcgccaggt
     3601 tctcattgag cgtaatgagc gtcccttggc tgtccatgat gaaagccgcg tcgggtggag
     3661 tatcgaaaaa gacgcggaca ccatcatctc ccccggagga tacaacctgt tccttctccg
     3721 gatcataatc gttcataggc acctcacaac aggggtaccg tgccccaact ccaaggggga
     3781 cggacacacc cgcctcgttc gtatctttcc tgaatcc
//
LOCUS       DAOPOA010000132         3811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_26057_length_3811_cov_2.597710, whole genome shotgun sequence.
ACCESSION   DAOPOA010000132 DAOPOA010000000
VERSION     DAOPOA010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26057_length_3811_cov_2.597710"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1990
                     /locus_tag="PKJ17_03880"
     CDS             <1..1990
                     /locus_tag="PKJ17_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOD75142.1"
                     /translation="VPYLSFPPIEPGTVFTYEFPIRQSGTYWYHSHTGTQQQVGVYGA
                     MVIEPIQDASKPDHDHVVMLSDWTDEEPHEVSRTLKRGSEWYALKKGSAQSIFGAASL
                     GRLGDYFARELQRMPAMDIADVAYDRFLVNGKPETMLPAAPGETVRLRIIDGSSTTYF
                     HLEFAGGPMTIVAADGQDVEPIQKTRFLIGAAETYDVLVHVPSAGMHEFRATAHDGSG
                     YASVWIGAGERNPAPDLPKPNLYHGMGHHGLKQIFSLTPAGTMGMPDHDVEAGKFDQP
                     GMMGMKGVHDSGDPSGMKMDGMDHGTHSMPVPTQTRPMDKEHGACSGLLGEDVCASKN
                     LAVDGVDPSRPWPPYAGLRSASQTVFPRGRPVREIRLTLDGDMERYVWFLNNKPLSES
                     DSIHIREGEVVRFIMINRTMMHHPMHLHGHFFRVINDQGDHAPLKHTVDVAPMSTTVI
                     EFDANEFGDWFFHCHLLYHMEAGMARVVHYEGFTVTPHLAAIRPELYRDPWYFWGQAD
                     VLSNMTKGFLTLSNTRNILTAEWEVGWHNVDDTRSEVLFTWDRYINRFLSVFAGADVL
                     GERSETDTRGIVGLRYLLPLNLESRAWIDTDGGARFNLGRTFQLTPRLGLFGEARYDT
                     HDDWEGVAGLSYTIDKIFSLIGQWHSDYGFGGGLQVRF"
     gene            2246..2575
                     /locus_tag="PKJ17_03885"
     CDS             2246..2575
                     /locus_tag="PKJ17_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75143.1"
                     /translation="MKRNVVVLSIVFLLVAAVCFAADYKKDIVGSWTYTFKGADSKID
                     HNADGTFVLVTKNITVKGTYGVEGNKLTLTTPEGKKIGYTIQSYSGKKMTIKRDADGR
                     EIVYNKK"
     gene            complement(2690..>3811)
                     /locus_tag="PKJ17_03890"
     CDS             complement(2690..>3811)
                     /locus_tag="PKJ17_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75144.1"
                     /translation="KMESIGTLAGGIAHDFNNILSALMGYASLMQIKMEKDNPLRPYL
                     DQILSASRKAADLTQSLLAFSRKQPVTLLPLDINSTIGSTKKLLKRLLTEDIDLSTSL
                     TRDDTVVMADRSQVDQILFNLVTNARDAMPRGGTLTIKTDIAEIDERFTRTHGFGSPG
                     QYVVITILDTGEGMDEATRRKIFDPFFTTKETGKGTGLGLATVYGIVKQHNGYITVQS
                     EPGRGSAFRIYLPAVRAKTDHEQEAAIPNDPGTGTILIAEDNQEVRRFIREALREYGY
                     TTIEAVDGEDAVSKFREHGDIDLVIVDSVMPKKNGREVYEEVHRMDPRARVLFMSGYT
                     KDVILEKGIEDGGLDFIAKPLTLDRLLKKVRELLAGQTG"
BASE COUNT          794 a         1107 c         1068 g          842 t
ORIGIN      
        1 cgttccctac ctcagctttc cgcccatcga acccggtacc gtctttacgt atgagttccc
       61 catccgccag agcgggacct actggtatca ttcccatacc ggtacgcagc agcaggtcgg
      121 tgtttacggc gccatggtca tcgagccgat ccaggacgcc tctaagcctg atcatgatca
      181 tgttgtcatg ttgtccgatt ggaccgacga ggagccgcac gaggtcagtc gaacacttaa
      241 acgcggcagt gagtggtatg ctcttaaaaa aggaagtgct cagagtatct ttggcgccgc
      301 cagcctcggc aggctcggcg actatttcgc acgtgaactc cagcgcatgc cagccatgga
      361 cattgcggac gtagcctacg accgcttcct cgtaaacggc aagcccgaaa ctatgctccc
      421 tgcggcgccg ggtgaaaccg tgcggctgcg cattattgat ggttcttcaa ccacctattt
      481 ccaccttgaa tttgccggcg gcccgatgac catcgtcgcc gcggatggtc aggacgtgga
      541 gccgatccag aagacacggt ttctcattgg tgctgccgag acctatgatg tgctcgttca
      601 cgtcccctcg gccggcatgc atgaattccg ggccacggcc cacgacggct cgggttatgc
      661 atcggtatgg ataggagccg gcgaacgcaa cccggctccc gatctgccta agcccaacct
      721 ttatcacggc atgggtcatc acggcctgaa acagatcttt tccctcaccc ctgcaggcac
      781 tatgggcatg cccgatcacg atgtcgaagc cggaaagttc gaccaaccag gaatgatggg
      841 catgaaaggc gtgcatgaca gcggtgatcc ttctggaatg aagatggacg gcatggacca
      901 cggtacccac tccatgccgg tccccacaca gacccgtccg atggacaagg aacatggcgc
      961 ctgctccggc ctcctcgggg aggacgtctg tgcgtcgaag aatctggctg ttgatggagt
     1021 ggacccgagc cgaccatggc cgccgtatgc tggactgcgg tccgcttcac aaactgtttt
     1081 tccaagggga agacccgtcc gcgaaatccg cttgacgctt gatggagata tggagcgcta
     1141 cgtctggttc ctcaacaaca agccgctttc ggaaagcgac tccatccata tccgcgaagg
     1201 cgaggtcgtg cgctttatca tgatcaaccg caccatgatg caccatccca tgcatctgca
     1261 cggccatttc ttccgcgtga tcaacgatca gggagaccac gcgccgctca aacacaccgt
     1321 cgacgtcgcc ccgatgtcca ccacagtgat cgaattcgac gccaacgagt tcggcgactg
     1381 gttttttcac tgccatctgc tctatcacat ggaggccggt atggccagag tcgttcatta
     1441 tgagggtttt accgttaccc cccaccttgc cgcgatccgg cccgagctct atagggaccc
     1501 ctggtatttc tggggccagg cagatgttct cagcaacatg accaagggct ttctgactct
     1561 ttccaacacc cgcaatatcc tgactgctga gtgggaagtg gggtggcaca atgtagatga
     1621 caccaggtcg gaagttcttt tcacctggga ccgctacatc aatcgcttcc tcagcgtatt
     1681 tgccggtgcc gatgtcctgg gcgagaggag cgaaacggac acccggggga tagtgggact
     1741 tcgttatctt ctgccgctca atctggaatc tcgcgcgtgg atcgataccg acggaggcgc
     1801 gaggttcaac ctgggtagaa cttttcaact aacaccgcgt ctcgggttgt ttggggaggc
     1861 ccgatacgat acccacgatg actgggaggg agttgccggt ctatcgtaca caatcgacaa
     1921 gatcttctcc cttatcggtc aatggcactc cgactatggt ttcggtggcg gcctgcaggt
     1981 ccggttctga gatggccatt ccatgaatac caggggaagc tttgagacgc cctttcattc
     2041 tcgcatgcag gtctgagcgg caaggagtcc actgggatgc ccctctggtt ccgttcgcca
     2101 taggcaccgg aaagctcctc gtcatacagg aaaggggcga tccgctgtca gaaggagaca
     2161 aagaaatatg ttaagaattg gcctgcaggt gacgatattt ctctgtggca tcggtggtac
     2221 aaaaaaacca acaggagagt gcataatgaa gagaaatgtc gttgtcttgt cgatcgtttt
     2281 cctcctcgtc gctgctgtct gctttgcggc cgactacaaa aaggatatcg tcgggagttg
     2341 gacgtacacc tttaaggggg ccgactcaaa gatcgatcac aatgccgacg ggaccttcgt
     2401 cctcgtaacc aagaacatca ccgtcaaggg aacctacggg gtggagggta acaagctgac
     2461 gctgacaacc ccggaaggca aaaagatcgg ttacacgatc cagtcataca gcggcaagaa
     2521 gatgacgatc aaacgcgacg ccgacggccg ggagatcgtt tataacaaga aatagaggaa
     2581 gcggacaccc cgcagcggtc ggtcctgcct tccggcaacg gacaagccgc tgcgggatcc
     2641 cactttgcgg cacagagtga gctgagcgca gcgggtgtgg accgctgttt cacccggttt
     2701 gcccggccag gagttcgcgg accttcttga gaagcctgtc gagtgtcagg ggcttggcga
     2761 tgaaatcgag cccgccgtcc tctatccctt tttccaggat gacatccttc gtgtatccgc
     2821 tcataaagag cacccgcgca cgggggtcca ttcggtgtac ctcttcgtag acctcccgtc
     2881 cgttcttttt cggcatcacg gaatcgacga ttacgaggtc aatatcccca tgttctctga
     2941 acttgctgac cgcgtcctcg ccgtccacgg cctcgatggt agtatacccg tattcgcgga
     3001 gggcctcccg tatgaaccgt ctgacctcct ggttgtcttc ggcgatgagg atcgtgccgg
     3061 tccccggatc gttgggaata gccgcttcct gttcgtggtc cgtcttggcc cgcaccgcgg
     3121 gaagatagat gcggaaggcg ctgccgcggc ccggctcgct ttgcacggtg atatacccgt
     3181 tgtgctgttt cacgataccg tacacggtgg caaggccgag acccgtgcct ttgcccgtct
     3241 ccttggtggt aaagaagggg tcgaagatct tccgccgggt cgcctcatcc atgccctccc
     3301 ctgtatccaa aatggttatc accacgtatt gtccgggcga gccaaaccca tgggttctcg
     3361 taaacctctc gtcgatctcc gcgatatccg tctttatggt gagcgtaccc ccgcgcggca
     3421 tggcatccct agcgttggtg acgagattga agaggatctg gtccacctgc gacctgtccg
     3481 ccattaccac cgtatcatcc cgggtaaggg acgtgctcag atcgatgtcc tcggtcagaa
     3541 gcctcttgag gagcttcttt gtcgatccta tcgtactgtt aatgtccagg ggaagaagag
     3601 tcacgggttg cttcctgctg aaggccagaa gactctgggt aaggtcggcc gccttccggg
     3661 aggccgacag gatctggtcc aggtatggcc gaagaggatt gtccttttcc atctttatct
     3721 gcatgagaga ggcgtatccc atcagggccg agaggatatt gttaaagtcg tgtgcgatgc
     3781 cccccgccag ggtgcctatc gattccatct t
//
LOCUS       DAOPOA010000133         3805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_26118_length_3805_cov_3.357333, whole genome shotgun sequence.
ACCESSION   DAOPOA010000133 DAOPOA010000000
VERSION     DAOPOA010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26118_length_3805_cov_3.357333"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /gene="murB"
                     /locus_tag="PKJ17_03895"
     CDS             <1..196
                     /gene="murB"
                     /locus_tag="PKJ17_03895"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="catalyzes the reduction of UDP-N-acetylglucosamine
                     enolpyruvate to form UDP-N-acetylmuramate in peptidoglycan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOD75145.1"
                     /translation="EKAGLRGYRMGDACVSEKHTNFIVNMGHARATDIAALIRKVKEE
                     VFSKLGVTLEEEVELWGFNG"
     gene            189..1097
                     /locus_tag="PKJ17_03900"
     CDS             189..1097
                     /locus_tag="PKJ17_03900"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOD75146.1"
                     /translation="MDRTGLKKLKIGVLLGGRSSEREISLKSGAAVLQSLVRSGYDAV
                     AIDARDRLVEKLKKQKVDAAFIVLHGRWGEDGTVQGLLEIMGIPYTGPGVLGSSAAMD
                     KAVMKFILEATGIPTPAYAIAGAGERITMKPPFVVKPANEGSTIGISMVHRRKDTAAA
                     MELALRYDRKIVIEEFVPGQEITVAVVNGKVLPVVEVRPLSGFYDFESKYTKGKTEYI
                     VPAEIPRKVAKKAEAIAMDVYRRFDLSGCVRTDMLVRDGMPLVIDINTSPGMTETSLV
                     PKAWACQGGSFDELIEEILGGASLKT"
     gene            1094..1795
                     /locus_tag="PKJ17_03905"
     CDS             1094..1795
                     /locus_tag="PKJ17_03905"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOD75147.1"
                     /translation="MKKLLYILFIGPVCILSMLSIAYLFSREEPLFFLQNIKVNGISQ
                     LGDKETMAKIAPHLTESIFKVDVAKVRDALTSHPFVKEVSVKRVYPFSIVIDVKEKIP
                     SALWVAGDGTVHILDEKGEPYRGLGKDEVKGLYLISTRERSDAKKVFSETNVFITEGI
                     LKKGQLSEVSYRDGDLTVFGLDDGVEIILGKEDHKKRLKRALAVLKDAGKRGLVIKCI
                     DARFEKGAIIQERKG"
     gene            1792..3045
                     /gene="ftsA"
                     /locus_tag="PKJ17_03910"
     CDS             1792..3045
                     /gene="ftsA"
                     /locus_tag="PKJ17_03910"
                     /inference="COORDINATES: protein motif:HMM:NF007009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOD75148.1"
                     /translation="MIRVKDDEQLLVGLDIGTTKICVVVGKASENQVNIIGIGSHPST
                     GLRKGVVVNMDSTVNSIKKAVEEAELMAGIKIDSCLAGIGGAHIKSFNSNGVVAVKDR
                     EVRPDDIDRAIDAAKAVAIPADRELIHVIPQEFIVDDQDGIRDPVGITGVRLEVKVHI
                     VTGSISSAQNIIKCCRLAGLNVDDVILCQLASAEAVLTPEEKEIGVAVVDIGGGTSDI
                     AVYANGAIKHTSVLPFGGNNITNDIAIGLRTPIDDAEKIKKKFGCAMASLVGQNETIE
                     VPSVGGRKPRTLQRKTLADIIEPRVEEVASLIYEEIKKSGLEKLLASGVVLTGGCSNL
                     DGLPEIAENIFNLPARKGDPIGVGGLIDVVNNPAYATAVGLLLFGFKEGRGKKRGFDA
                     TRSMKRLFSNQKLLTKMKDWFKEIF"
     gene            3065..>3805
                     /gene="ftsZ"
                     /locus_tag="PKJ17_03915"
     CDS             3065..>3805
                     /gene="ftsZ"
                     /locus_tag="PKJ17_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOD75149.1"
                     /translation="MFYMDESNGFSAKLIVVGVGGGGCNALNNMVEAGVQGVEFIAVN
                     TDIKSLSTCKAPVKIQIGTKLTEGLGAGANPEVGKKAALEDVDKLKDHLKGAHMVFIT
                     CGLGGGTGTGASPVIAEIARELGALTVAIATKPFAFEGKDRMNQSEGGVAQLKSRVDS
                     LITIPNQRLLSIGGKHMTIREAFLKADEVLLNAVRSISDLIVGSGHVVVDFADVKTIM
                     SERGMAIMGIGESAGENRARDAAQKAISS"
BASE COUNT          885 a         1084 c         1218 g          618 t
ORIGIN      
        1 ggaaaaggcg ggcctgagag gataccgcat gggcgatgcc tgcgtctcgg agaagcacac
       61 gaacttcatc gtgaacatgg gccacgcccg ggcaaccgac atcgcggccc ttatcaggaa
      121 ggtaaaggag gaggtcttct cgaagctcgg ggtcacgctc gaggaagagg tggaactctg
      181 ggggttcaat ggatagaacg ggtctcaaga agctgaagat aggcgtcctc ctgggaggaa
      241 ggtcgtcgga acgcgaaata tccctgaaga gcggcgcggc cgtccttcaa agccttgtca
      301 ggtcgggata cgacgcggtg gccatcgacg ccagggaccg ccttgtcgaa aaactgaaaa
      361 agcagaaggt cgacgcggcc ttcatcgtcc tccacggcag atggggagag gacggcacgg
      421 tccagggcct cctcgagatc atgggaatac cctacacggg gcccggcgtc ctcggctcgt
      481 cggctgcgat ggacaaggcc gtcatgaaat tcatcctcga agcgacgggg atcccgacac
      541 cggcctacgc aatagcggga gcgggcgaaa ggatcaccat gaagcccccc ttcgtggtga
      601 aacccgccaa tgagggatcg acgatcggca tatcgatggt ccacaggagg aaggacaccg
      661 ccgcggccat ggaactcgcc ctcagatacg acaggaagat cgttatcgag gagttcgtgc
      721 ccgggcagga gatcaccgtg gccgtcgtca acggaaaggt cctccccgtt gtggaggtca
      781 ggccgctgtc gggcttttac gatttcgaat cgaagtacac gaaggggaag acggaataca
      841 tcgtccccgc ggagataccg cggaaggtgg ccaagaaggc tgaggccatc gccatggacg
      901 tctaccgcag gttcgacctc tccggatgcg tgcggacgga catgctcgta agggacggga
      961 tgccccttgt catcgacatc aacacgtccc cgggcatgac ggagacatcc ctcgtcccca
     1021 aggcctgggc ctgtcaggga ggctcgttcg atgagctcat cgaggagata cttggagggg
     1081 cgtcgctgaa gacatgaaga agcttcttta catactcttc atcgggccgg tgtgcatcct
     1141 ttcgatgctc tccatcgcct acctgttctc ccgggaggag cccctgttct tcctgcagaa
     1201 cataaaggtg aacggcatct cccagctcgg cgacaaggag acgatggcca agatcgcacc
     1261 ccatctcacg gagagcatct tcaaggtgga tgtggcaaag gtccgggacg ccctcacatc
     1321 ccatcctttc gtaaaggaag tgagcgtgaa gagggtctat cccttctcca tcgtcatcga
     1381 cgtgaaggag aagataccgt cggccctctg ggtggcgggc gacggcaccg tccatatcct
     1441 cgacgaaaag ggcgagccct accgtggcct cgggaaggac gaggtaaagg gcctctatct
     1501 tataagcacc cgggagagat cggatgcgaa gaaggtcttc agcgagacga acgtcttcat
     1561 caccgagggt atcctgaaga aggggcagct ctcggaggtc tcctaccgcg acggcgacct
     1621 caccgtcttc ggtctcgacg acggcgtgga gatcattctc ggcaaggaag accacaagaa
     1681 gagactgaaa agggccctcg ccgtccttaa ggacgcaggc aagcggggtc ttgtcataaa
     1741 atgcatcgac gcgcgctttg aaaagggcgc cataatccag gaaaggaagg ggtgatccgg
     1801 gtgaaggacg acgaacagct cctcgttggg ctcgacattg gaacgacaaa gatctgcgtg
     1861 gtcgtcggaa aggcgtcgga gaatcaggtc aacatcatcg gcatcggttc ccacccgtcg
     1921 acgggccttc gcaagggagt tgtggtcaac atggacagca ccgtcaactc catcaagaag
     1981 gcagtcgagg aagcggaact catggccggc atcaagatcg actcgtgcct cgccggcatc
     2041 gggggtgcgc acataaagag cttcaactcc aacggcgtcg tcgccgtcaa ggacagggag
     2101 gtccgtcccg acgacatcga cagggccatc gacgccgcga aggcggtggc gatccccgcc
     2161 gacagggaac tcatccacgt catcccccag gagttcatcg tcgacgacca ggacgggatc
     2221 agggaccccg tggggataac gggggtaagg ctcgaggtga aggtccacat cgtcacgggc
     2281 agcatctcct cggcccagaa catcatcaag tgctgcagac tggcggggct caacgtcgac
     2341 gacgtcatac tctgccagct tgcctccgca gaggcggtcc tcacacccga ggagaaggag
     2401 ataggcgtcg ccgtcgtgga catcgggggc gggacgagcg acatcgccgt gtacgcgaac
     2461 ggcgcgataa agcacacgtc cgtcctgcct ttcggcggga acaacataac aaacgatatc
     2521 gccataggtc tgagaacccc catcgacgac gcggagaaga taaagaagaa attcggctgc
     2581 gccatggcga gcctcgtcgg ccagaacgag accatcgagg tgccaagcgt cggcgggcgc
     2641 aaaccccgga cgctccagcg caagacgctg gccgacatca tagagcccag ggtcgaggag
     2701 gtggcctcgc tgatctacga ggagatcaag aaatcgggcc tcgagaagct ccttgcctcc
     2761 ggcgtcgtgc tgacgggagg gtgctcgaac ctcgacggtc tgcccgagat agcggagaac
     2821 atatttaacc tcccggcgcg gaagggagac cccataggcg tcggaggcct tatcgacgtc
     2881 gtcaacaacc ccgcctacgc gacggcggtg gggcttctgc ttttcggctt caaggaggga
     2941 cggggaaaga aacgtgggtt tgacgcgaca cggtcgatga agagactctt cagcaaccag
     3001 aagctgctca cgaagatgaa ggattggttc aaagaaatat tctaggaggt gcgaggtgag
     3061 tggaatgttc tacatggacg agagcaacgg attttcagca aaactcatcg tggtcggcgt
     3121 tggaggaggt ggctgcaacg cgctcaacaa catggtggaa gcaggcgttc agggcgttga
     3181 gttcatcgcc gtcaacaccg acatcaagtc gttgagcacg tgcaaggctc ccgtcaagat
     3241 ccagatcggc acgaagctga cggagggcct gggagccggg gccaatcccg aggtgggcaa
     3301 gaaggccgcc ctcgaggacg tggacaagct caaggaccac ctgaaaggcg cccacatggt
     3361 cttcatcacc tgcggccttg gcggcgggac gggaacgggt gcatcccccg tcatcgccga
     3421 gatagccagg gagctggggg ccctcaccgt ggccatcgcc acgaaaccct tcgctttcga
     3481 ggggaaggac aggatgaacc aatccgaggg cggcgtggcc cagctcaagt cacgcgtcga
     3541 ctcgctgatc accatcccga accagaggct cctttccatc ggcggcaagc acatgacgat
     3601 aagggaggca ttcctcaagg ccgacgaggt cctgctcaac gcggtgagaa gcatctccga
     3661 cctcatcgtc ggctccggcc atgtcgtcgt cgactttgcc gacgtgaaga cgatcatgag
     3721 cgaacggggc atggcgatca tgggcatcgg cgagtcggcc ggcgagaaca gggcccgcga
     3781 tgccgcccag aaggccatat cgagc
//
LOCUS       DAOPOA010000134         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_26141_length_3803_cov_3.414621, whole genome shotgun sequence.
ACCESSION   DAOPOA010000134 DAOPOA010000000
VERSION     DAOPOA010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26141_length_3803_cov_3.414621"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PKJ17_03920"
     CDS             <1..433
                     /locus_tag="PKJ17_03920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD75150.1"
                     /translation="PVIGHLSDRTLKSRKKILVWSSALNVICFACAVLFFDGLGLVAL
                     YVLFFFMGITISSVGTVAIVTTKELFPDEIAGTSMGTINLFPFIGAIIFQPLMGYVLD
                     RAGMTGGSYPPSAYKQMLWIYLITSILALFSIMKCKETFKG"
     gene            731..2467
                     /locus_tag="PKJ17_03925"
     CDS             731..2467
                     /locus_tag="PKJ17_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HOD75151.1"
                     /translation="MIDKTLQDLKALVAVASAEREPDVIIEGGEVFDVFTGEVFPGDL
                     WICGNWIAYVGEKRTKTGKDTQVVDARGLVAVPGYLDAHGHADIFYNPATFSDVAVTK
                     GTTTVFSDSHDLMSCLGPKGFAEVLRKARYFNIKYLWGVPATYPPYPEVEGGEMFSIY
                     DIWDLFSRHPECASVSEVSSYLRIIRNEDEILERMLLARSLGRNVEGHTLGASYDRLN
                     ALVAAGITSCHESIRPADLKNRIRLGLYTMVRHSSIRTDLEELGPAINTLPNDSIILV
                     SDGIFADDLMFRGYMDHIVGEAIRFGVEPQNAIRMATINPARYFRVDSEVGSLTPGRI
                     ADVLLLEGLDRPVPVKVFERGTLAAEGGTLTRRPSPELEVGVEFYPYAFSSVGASEFV
                     VERPDVKTVPVIDIVDRTVTRRMDVALSRHGNIILPDRKGDVRKIMYTRREQKKWGQG
                     FLHGFGADCGGIASTVAHETHGLLVHGFDDDDMALAANTVLAMKGGIAVVDAGKVLHA
                     LRLPLGAIMSRVPLPELDRALRKLVDLLHSLGNTLDDPLWTMGFLPFTSIVELRITVS
                     GTYDVRKGSIVF"
     gene            2731..3803
                     /locus_tag="PKJ17_03930"
                     /pseudo
     CDS             2731..3803
                     /locus_tag="PKJ17_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
BASE COUNT          852 a         1077 c         1126 g          748 t
ORIGIN      
        1 ccccgtcatc ggccaccttt cggacaggac gttgaagagc aggaagaaga tcctcgtatg
       61 gtcctcggcc ctcaatgtta tctgttttgc ctgtgccgtt cttttcttcg acggtctcgg
      121 gctggtcgcg ctctatgtgc tcttcttctt catggggatc acgatcagct ccgtcgggac
      181 cgtcgccatc gtcaccacaa aagagctttt ccccgatgag atagcaggca cgtccatggg
      241 caccatcaac ctgttcccct tcatcggggc catcatcttc cagccgctta tgggctacgt
      301 tctcgacagg gcgggcatga cgggcggctc ctacccgccg tccgcgtaca agcagatgct
      361 ctggatatac ctcatcacca gcattctggc ccttttcagc attatgaaat gcaaggaaac
      421 cttcaagggc taaaggcgtt caaggatgaa gaaggttcaa gagttcaaaa gttcaagagt
      481 tccagggtca agaacaggaa cagaatgtcg cgcaggtcgc gaagtgtctt gacaaaaaag
      541 gcaccggcgc cttcggccgg tgtacgaatt cccttggcag ccgacccccc tcgcggtctt
      601 tcccttgaac ccttgaatcc ttgaaccctt gaacccttga accattttcg aatctcttgc
      661 catcgaaggg ggttttgggc tacattgaaa cgtactccaa gggaaagatg accggcagga
      721 cagaaccata atgatcgaca agacactcca ggacctgaag gccctcgtgg ccgttgccag
      781 cgccgagcgg gagccggatg tcatcataga aggcggcgag gtgttcgatg tcttcacggg
      841 agaggtcttt cccggcgacc tgtggatctg cggcaactgg atcgcctatg tgggggaaaa
      901 gagaacgaag acagggaagg acacccaggt cgtcgacgcg cggggtcttg tcgccgtacc
      961 cggctacctg gacgcccacg gacacgcgga catattctac aacccggcga cgttttccga
     1021 cgttgccgtg acgaagggga caacgaccgt cttttccgac agtcatgacc tgatgagctg
     1081 tctcgggccc aaagggttcg ccgaggtcct cagaaaggcg cggtatttca acatcaaata
     1141 tctctggggc gttccggcga cctatccacc ctatccggag gtggagggcg gcgagatgtt
     1201 ctccatctac gatatctggg accttttttc gcgccacccc gagtgcgctt ccgtgagcga
     1261 ggtctcatca tacctgagga taataaggaa cgaggacgag atactggaaa ggatgctcct
     1321 tgcccggtcc ctggggagga acgtcgaggg tcatacgctt ggcgcctcct atgaccggct
     1381 gaacgccctt gtcgcggcgg gcatcacatc ctgccacgaa tcgatccgcc ccgcggacct
     1441 gaagaaccgg atacggctgg ggctttacac gatggtccgc cacagctcca tcaggaccga
     1501 cctcgaggag ttgggccccg ccataaacac ccttcccaac gattccatca tcctcgtgtc
     1561 cgacggcatc ttcgccgacg atctgatgtt caggggatac atggaccata tcgtgggcga
     1621 ggccatccgt ttcggggtcg agccgcagaa cgccatccga atggccacca tcaatccggc
     1681 caggtacttc agggtggatt cggaggtggg cagcctgacg ccgggcagga tagcggacgt
     1741 gctccttctc gagggcctcg acagacctgt ccccgtcaag gtcttcgagc gcgggaccct
     1801 tgccgcggag ggagggacgc tcacgcgtcg gccgtcaccg gagctcgagg tcggggtaga
     1861 gttctaccct tacgcgttct cgtccgttgg ggcctccgag ttcgtcgtgg agcggccgga
     1921 cgttaagacc gttcccgtca tagatattgt cgaccgcacc gtgacgaggc ggatggatgt
     1981 cgcgctgtcc cggcacggca atatcattct ccccgacagg aaaggcgacg tacggaagat
     2041 catgtacacg cggagggagc agaagaagtg gggccagggg ttccttcatg gcttcggggc
     2101 cgactgcggg ggtattgcct caacggtggc ccatgaaacg catggtctcc tcgttcacgg
     2161 tttcgatgac gatgacatgg ctctggcggc gaacacggtg ctcgccatga aaggggggat
     2221 agccgtcgtt gacgccggca aggttcttca tgccctgcgg ctgccgctcg gcgccatcat
     2281 gtcccgcgtc cccttgcccg aactggaccg tgcccttcga aagctcgttg accttctcca
     2341 ctctctcggg aacacccttg atgaccccct ctggaccatg ggctttctgc ctttcacctc
     2401 catcgtggag ctgaggatca ccgtttcggg aacctatgat gtcagaaaag gttcgatagt
     2461 gttttgaaga aggacaagaa gaaaaaaaag aagaagaaga gttcgacggg aaagatctac
     2521 aagtatctgc tggtcctgct cgtcgcgtcg gcggtgctct tcgtcgcggc cttcatcttc
     2581 tttattatca acgatatacc ctccgtcgtg gcgctgaagg acctcaagaa caagcccgtc
     2641 accagcgttt acgacgtcaa tgacgaactc acctacctgt ttgtacccga caacagggtc
     2701 ttcgttacct acaaacggat cccaaagcat gtgagggccg cctttttggc ggcggaggac
     2761 gccgatttct tcaagcacgg gggcgtcgac ccgatgggca tcctaagggc attcataaga
     2821 acgtggcata cggccggatg gcccaggggg gcagcaccat cacacagcag gtcatcaagt
     2881 caatgatact gggtcctgaa aagagcctga acaggaagat acgcgaggcc atcctggcat
     2941 acaagctgga gaactacctg agcaagaacg agatcctcaa tctttacctc aacaacatct
     3001 acatgggaca cggtgtctat ggcgtggagg ccgcgtccca ggtgtacttc ggaaagcatg
     3061 tctggaacat aacgcccgcc gaggcggccc tgctggcagg tatcgtgcag gcgccctccc
     3121 ggtatacacc caaaaggcat ccgggaaatg cccggatgag acaggaatac gttatcgacc
     3181 agatggtgga gaagaagttc atcagcgaga aggcaaagca gcagatgctc aaggaaaaga
     3241 tcgatatcag ggaggacgac ggcgtctttg ccgacagcta ctacaaggac cacatactcc
     3301 ggtatgtgga ggcccgctac ggcaaaggcg ttctgtccag gaagggactc aagatctacg
     3361 cggccgtgga cagacggatg caaaagaccg ccgaggacgc gataaagagg ggtctcgagg
     3421 tctatgaacg cagaaagggc gaatacacgg tcctctacca cctggggcag aagaagtggg
     3481 atgacctgaa gcgggcggaa gagcgcgacc gcaaactggt gcccgtcagc aagggacgga
     3541 cctgcagtct cctggtatcg gaaaggataa aggaagggta cgccgtcttt gtaggcgagc
     3601 agaaggccgt cctcaagatg gacacccacc ctttcaaacc cggcgatgtg gtgaaagggt
     3661 tttgcaccgg tgaggacagg aagaagacgc cggtcttcga ggccgttcgc tcgctgaagg
     3721 tcgagggcgc ccttgtgagc ctcgacgtca atctgggata cgtctacgcc atagtcggag
     3781 gaagggactt cgagaggagc ccc
//
LOCUS       DAOPOA010000135         3795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_26219_length_3795_cov_3.972460, whole genome shotgun sequence.
ACCESSION   DAOPOA010000135 DAOPOA010000000
VERSION     DAOPOA010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26219_length_3795_cov_3.972460"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKJ17_03935"
     CDS             <1..291
                     /locus_tag="PKJ17_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75152.1"
                     /translation="FVSGGPRAVKVERGPFRPFPREGYCLRLLCRRSFLLPPSVQGER
                     APVVIGPGNDNVSFELLPEDGKVFGLLLVMASSSFGVSEEECPGSVVIKVAS"
     gene            complement(210..428)
                     /locus_tag="PKJ17_03940"
                     /pseudo
     CDS             complement(210..428)
                     /locus_tag="PKJ17_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612386.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(813..1658)
                     /locus_tag="PKJ17_03945"
     CDS             complement(813..1658)
                     /locus_tag="PKJ17_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD75153.1"
                     /translation="MRQIAIYGKGGIGKSTIASNLSAALHEMGHTVMQVGCDPKRDST
                     RILAGGKFIPAVLEEHREQLRVGKDEYSIKLDNIVYRSPGGIYLVEAGGPEPGVGCAG
                     RGVLTALQILKDLNAFSTYDIDVAIYDVLGDVVCGGFSMPIREGFAREIYLVCSGGFM
                     SIYAANNIARAVQRLAKRGDTGLAGIICNSNGDEEFEHRVLPAFAAMLGTSFIQYVPR
                     SPVIQACELERKPVVEHAPSSREAGVFRDLARAVMENDSRVIPTPVSDLAELEDMYRQ
                     HVQKR"
     gene            complement(1667..3025)
                     /locus_tag="PKJ17_03950"
     CDS             complement(1667..3025)
                     /locus_tag="PKJ17_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOD75154.1"
                     /translation="MEQRPDKGPAGTAAGSLDQCTDPYLRCAFNGTAQTALGVSRSAL
                     LAHSPQGCDYLVNNAFASQQCDYMETVTLCTKLCVDEIVHGGEDLLARTIREARDLPI
                     SVLFVISACGPEIVGDDIVAVCEAVQPEVKFRLLPIECAGFRGSQYDGIDIALDTMLR
                     KLVRGSGKKVPGSVCLIAPHANANPTWPADLAWVRQVLCRMGVPVVATLTHRTPLSEI
                     ENAASAEASIVLSHDAGQKAADYLHATYGIEQVCRGIPLPIGMTNTQRWLTALGERFD
                     ALKIAEEMVSKGERMVMETCRRKWPMARFLYRVPAAIVADATVGIPLVRFVTEEMEMT
                     PQLVALYSSQRQVRELLDRELESLGLSPKVALGTDVYKTRKCLADARPRVVFGSTIER
                     HASEGLSIPYIVEVVRMMRQFRLIDREYFGYGGVLNLFECVQNEWTMAWRSKEKRYQA
                     KW"
     gene            complement(3006..>3795)
                     /locus_tag="PKJ17_03955"
     CDS             complement(3006..>3795)
                     /locus_tag="PKJ17_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOD75155.1"
                     /translation="LSDIGIRVNAVIAGGCTVEQIRHAREVELNASWCYDWGQKLGDL
                     MQERFGVPYSRTGQPYGPTATKEWLLGVARPLGMEEKALEVIEREMREVEPDLAYMRQ
                     ALEGRTAVIEISEFPGPIRALSLARMAEEFGAHPVVINVHPYTIKERMPSIKYILETG
                     VNPEIILTKGLFHLGSFRSSKETEDELEEIVRGYDNVIFFGNSGRFPPCPVVNLTLMN
                     PAFQPHYGFRGIKNIAALIRQALENENLPRSRLFRGMLYGTTAR"
BASE COUNT          664 a         1246 c         1086 g          799 t
ORIGIN      
        1 tttgtgtccg gcggcccgcg cgcggtcaaa gtagagcggg gaccgtttcg tcccttcccc
       61 agggagggct attgtcttcg gcttctctgc cggcggagtt ttctccttcc accttccgta
      121 cagggtgagc gggcgcccgt ggtcatcggc ccagggaatg acaatgtatc cttcgagctt
      181 cttccagagg acggcaaagt cttcggcctc cttctcgtca tggcctcctc gtcttttggc
      241 gtatcggaag aggaatgccc tggatcagta gtgataaaag tcgcatcctg aatcacaccc
      301 tgtttaatct ttaagccctt cgcatcgagt tggtgttgaa gttgatccca gatctcctgt
      361 tctttccctg cctcgatcag ccgttctctg aaatatcata ttgttgtgta cacgctcgag
      421 ggcacaagcc gaaagctgat ggatctgctt cccatgttct ggtaggtgag gcacttcaga
      481 gcgaggaggg gggcgtcata cgggtacgtt atcctcgctc tgtgaagaag gacttccagg
      541 gcagggaaac cagcggtcga gaccagcgta cctttttttc gtattttcaa agacctgttc
      601 ggatttattc ccgccatgaa cgggatatcg gcgaggttcg ataatacccc tggtaataca
      661 gaccctgccc aacatttttc atgaactggc ggtagaggta taccgctggt ccgaccggca
      721 aacagcttcc tgcccgacac cttttcccag cgatgtccac tccacggcct gccgatggac
      781 acaggtcact gtaccgcacc cgtccgggtt cgtcacctct tttgcacgtg ctgccggtac
      841 atatcctcga gttccgcaag gtcgctcacc ggcgttggga tcacccgcga atcgttctcc
      901 atgaccgccc ttgcaaggtc ccggaacacg ccggcttccc tggaagaggg agcgtgctct
      961 accacgggtt ttctctctag ttcgcacgcc tggatcacgg ggctgcgcgg gacgtactgg
     1021 atgaacgacg tccccagcat cgcggcaaac gccgggagga ccctgtgctc gaactcctcg
     1081 tcgccgttgc tgttgcatat gatcccggca agcccggtgt ccccacgctt cgccaggcgc
     1141 tgcacggccc ttgcgatgtt gttcgctgca tagatgctca tgaacccgcc cgagcagaca
     1201 aggtagatct ccctcgcgaa cccctcgcgg atgggcattg agaacccgcc gcacacgacg
     1261 tctcccagca cgtcgtagat cgccacgtcg atgtcgtagg tcgaaaaagc gttcaggtcc
     1321 ttgaggatct gcagggctgt cagcacgccc cggccggcac acccgacacc tggctcaggc
     1381 ccgcccgcct cgacgaggta aatgccgccg ggcgacctgt acacgatgtt gtcgagcttg
     1441 atcgaatact cgtccttccc aactctcagc tgctcccggt gctcttccag cactgcgggg
     1501 atgaactttc cccctgcaag gatcctcgtc gagtccctct tggggtcgca gccgacctgc
     1561 atgaccgtgt gccccatctc gtgcagggcc gccgacaggt tcgaggcaat ggtactcttc
     1621 ccgatgcccc ccttgccgta aatcgcgatc tgtctcataa ctcctttcac cacttcgcct
     1681 ggtaccgttt ctctttcgaa cgccatgcca tcgtccactc gttctggacg cactcgaaca
     1741 ggttcaggac cccgccataa ccgaaatact cccggtcgat cagcctgaac tgccgcatca
     1801 tccttacgac ctcgacgatg taggggatac taagcccttc gctcgcgtgc cgctcgatgg
     1861 tgctgccaaa cacgacccgc ggcctcgcat ccgcaaggca tttcctcgtc ttgtaaacgt
     1921 ccgttccgag cgcgactttc ggagaaagac cgaggctttc cagttcccgg tccagcagct
     1981 cccgcacctg ccgctgggag ctgtacagtg cgacgagttg cggggtcatc tccatctcct
     2041 cggtgacgaa gcggaccagg gggatgccga ccgtggcatc cgcaacgatc gcggcaggta
     2101 cccggtacag gaaccgggcc atcggccact tcctccggca tgtctccatc accatccgct
     2161 cgcccttcga gaccatctct tccgcgatct tcagtgcatc gaaccgctcg ccaagcgcgg
     2221 tgagccagcg ttgcgtgttg gtcattccga tgggcagggg tatccccctg cagacctgct
     2281 cgatgccgta cgtcgcatga aggtaatctg ccgctttctg cccggcatcg tggctcagaa
     2341 cgattgacgc ctcggcggac gcggcgttct cgatctccga taggggggtg cggtgcgtca
     2401 gggtcgcgac gacagggacc cccatccggc agaggacctg gcgcacccac gcaaggtcgg
     2461 ctggccatgt cgggttcgcg ttcgcgtgcg gggcgatgag gcacaccgag cccggcacct
     2521 tcttaccgct ccccctgacc agtttccgga gcatcgtgtc cagggcgata tcgatcccgt
     2581 cgtactgcga ccccctgaac ccggcgcatt cgatcgggag gagccggaac ttcacctcgg
     2641 gctgcacggc ctcgcagact gcgacgatat cgtcgcccac gatctccggt ccgcaagcgc
     2701 tgatcacgaa caagacggaa atgggcagat cccttgcctc ccgtatggtc cgggcgagga
     2761 ggtcctcgcc gccgtgcacg atctcgtcca cgcacagttt cgtgcagagc gtcactgtct
     2821 ccatgtagtc gcattgctgc gatgcgaacg cgttgttgac gaggtaatca cagccctggg
     2881 gcgaatgggc gagcagggca gacctggaga ccccgagcgc cgtctgggcg gtgccgttaa
     2941 aggcgcaccg gaggtacggg tcggtgcact ggtccaggga gccggcggcc gtcccggcag
     3001 gtcctttatc gggccgttgt tccatagagc attccccgga acaggcgcga gcgcgggagg
     3061 ttctcgttct cgagcgcctg ccggatgagg gccgcgatgt tctttatccc ccggaagccg
     3121 tagtggggct ggaaggccgg gttcatgagc gtcaagttca ccacgggaca gggggggaac
     3181 cgccccgagt tgccgaagaa gatgacgttg tcgtacccgc ggacgatctc ttccagctcg
     3241 tcctccgtct ccttcgatga ccggaaactc cccaggtgga agagtccctt cgtgaggatg
     3301 atctccgggt tcacccccgt ctcgagtatg tacttgatgc tgggcatcct ctccttgatc
     3361 gtgtaggggt ggacgtttat cacgaccggg tgggcaccga actcctctgc catcctcgcc
     3421 agcgagaggg cccgtatcgg gccggggaac tctgatatct cgatgactgc agtcctgccc
     3481 tcgagggcct gcctcatgta ggcgaggtcc ggctccacct cccgcatctc ccgctcgatc
     3541 acctccagcg ccttctcctc catgccaagc ggcctggcca cccccaggag ccactccttc
     3601 gttgcagtcg gcccgtaagg ctgccctgtt cggctgtatg ggacgccgaa ccgctcctgc
     3661 atcaggtcgc cgagcttctg cccccaatca tagcaccacg aggcattgag ctcgacctcc
     3721 cgggcatgcc tgatctgctc gactgtgcag ccacccgcga tgacggcatt gacccgtatc
     3781 ccgatatcgg aaagg
//
LOCUS       DAOPOA010000136         3789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_26269_length_3789_cov_3.833958, whole genome shotgun sequence.
ACCESSION   DAOPOA010000136 DAOPOA010000000
VERSION     DAOPOA010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26269_length_3789_cov_3.833958"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..812
                     /locus_tag="PKJ17_03960"
     CDS             306..812
                     /locus_tag="PKJ17_03960"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOD75156.1"
                     /translation="MESVFTETKAEDIGRNPFRLIGADWMLITAGTKDSFNTMTGAWG
                     GFGIVWDKRVAICLVRPTRYTYEFMERSWSFSLCFFDEQYRDALTYCGTTSGRDVNKV
                     AEAGLTPVFGEDTTYFAEARLVIECRKIYRQDIEPQNFLAPEIDGFYPKKDYHRMYVG
                     EIIRCLSR"
     gene            985..1344
                     /locus_tag="PKJ17_03965"
     CDS             985..1344
                     /locus_tag="PKJ17_03965"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOD75157.1"
                     /translation="MRLEEEMEVFELQAEVCLALGNPRRLQILGLLKEGERSVAQMLE
                     RMGINKANLSQHLAVLRQKGLVISRREGGAVYYRLASPRITEACSIMREVLLETLRSR
                     ERISRSMLEGMGTVGEG"
     gene            1409..2101
                     /locus_tag="PKJ17_03970"
     CDS             1409..2101
                     /locus_tag="PKJ17_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD75158.1"
                     /translation="MGGEMGKGSAREGLERAKRSRRAKQVVLGTVFLALLVGGWFYSL
                     IGYFIPLCMVAGVGLASVRGRKWCNWMCPRGSFADTYMKLISPERKIPGWLRGTPVRL
                     GVLAFLMAMLAFQIVRLWPDPYAIGRFFVILLTVTTVVGVFMALFLHQRAWCSICPIG
                     SLSSWVGKDRYQLNMEKDACVNCGLCAKTCPMQLAPQEMKENEAMAFKGDCLKCGLCV
                     KACPKQALRFPG"
     gene            2364..>3789
                     /locus_tag="PKJ17_03975"
     CDS             2364..>3789
                     /locus_tag="PKJ17_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOD75159.1"
                     /translation="MNTVRDLKSEHCRGDSRVEVNNRKCRRCFYCVQLCPAKAIKLEK
                     ESIRIIPERCILCGNCITGCPHRAMTYHSDASIVRNLIEANENVVACLDPAFPAILDN
                     VSPWGFSYALKALGFKEVWEGAVGAELISGPYRKLLTETEKPVISSFCPVIVSYIEKY
                     LPQLIGNLAPIVSPMIAAGRAIKKAKGEGWRVVYIAPCLARMGELDYEENADAIDHVI
                     TFRDVRFMFAEAGINPRHVDEAPFDGPVPALGRIVPVIGGLNRSLGHSFDVLVGETSV
                     AYGRRRVMAALNQLAKGAIQARFFDLVFCDGCVDGSFVERELSVVGRRQIVSRYAKSQ
                     FSPQTASVLQRTLRDFADVKITKEHKANVQKLPDPTEEQIRAVLKRINKLPPHNNLDC
                     RACGYNTCREKAVAVVQGISEAEYCLPYLLEGSKRVYEQLEKSHRELQRSHQELEQAQ
                     FQLIRTEKLAALGQLAAGVAHEINN"
BASE COUNT          832 a         1014 c         1154 g          789 t
ORIGIN      
        1 aatcgccggg aaacaagacg acccgcttcc cgtaagattc ctcgcggact atcatttctc
       61 attgccacct ttacctccac cctgcaggag cccccggaac tgtacccctg cacactagta
      121 atcggctgac cggcgggaag cttaaacggc gaaaataatg ttttaggttt caagttttag
      181 gttttaagta aaaggagccg ggatggcaga ggaagacagc cgtggccttg caatcatgac
      241 caccgcgtaa tagtattgaa cttaaaacgt aacacctaaa actcaaaaca tctttcggag
      301 gttccatgga gagcgtgttt acggagacga aggcggagga tatcgggcgc aaccctttca
      361 gacttatcgg ggcggactgg atgctgatca cggcgggtac gaaggattcc ttcaacacga
      421 tgaccggcgc ctggggcggt ttcggcattg tgtgggacaa acgcgtcgcc atatgtctcg
      481 ttcgtccgac ccgctatacc tatgaattca tggagcgttc atggtcgttc agcctgtgct
      541 tcttcgatga gcagtaccgt gacgccctga cctactgcgg gacaacgtcg ggcagggacg
      601 tgaacaaggt cgccgaagcg ggtctcaccc ccgtcttcgg cgaggacacg acctacttcg
      661 ctgaggcgcg cctcgtcatc gagtgcagga agatctaccg ccaggacata gagcctcaga
      721 acttcctcgc accggagata gacggcttct acccgaaaaa ggactaccac aggatgtacg
      781 tgggcgagat catccgctgc ctgtcgaggt aggagaaacg ggaatctccc cgtgtcctat
      841 ccggtcttag agccctcacc acttcacccc ggtctgccct gccctcccaa tccccgaccc
      901 agccgggtct tgacagcagg cacaggttgt attatagttg cgatatcatg aaactacgca
      961 attaggaaat tgaaggagat ggtcatgagg cttgaggagg aaatggaggt gttcgagttg
     1021 caggctgagg tgtgcctggc gctggggaat ccgcggcggc tgcagatact gggcctgctg
     1081 aaggaggggg aaaggtccgt ggcgcagatg ctggaaagga tggggataaa caaagcgaac
     1141 ctgtcccagc atctcgcggt tctgagacag aaagggctcg tcatatccag gagggagggc
     1201 ggggcggtgt actacaggct cgcaagtccg aggataactg aggcttgctc gataatgcgc
     1261 gaggtgctgc tggaaacctt gcggagtaga gagcgcattt cccgaagcat gcttgaaggg
     1321 atgggaactg tgggagaggg ataggacgta taggagttga taggaggacc gataggacat
     1381 atgggaccta taggacttgg agggagttat gggtggtgag atggggaagg gttcggcgag
     1441 ggaaggactt gaaagggcga aacggtctag gcgtgccaaa caggttgttc tgggaaccgt
     1501 ctttcttgcg cttcttgtcg gagggtggtt ctattctctg atcgggtatt tcattccgct
     1561 gtgcatggtg gctggtgtgg gattggcctc cgttcggggc aggaagtggt gcaactggat
     1621 gtgcccccgg gggtccttcg ccgataccta tatgaagctc atcagtccgg agcggaagat
     1681 accaggctgg ctgaggggca caccggtgag gttgggcgtt ctggcattcc tcatggcgat
     1741 gcttgccttc cagatcgtgc ggctgtggcc ggacccgtac gcgataggga ggttttttgt
     1801 catactactc accgtcacga ccgtcgtggg ggtcttcatg gcgcttttcc ttcaccagcg
     1861 cgcctggtgt tccatctgcc ccatagggag cctatcgagc tgggtcggga aggaccgcta
     1921 ccagctgaac atggagaagg acgcctgcgt gaactgcggt ctctgcgcga agacctgccc
     1981 tatgcagctt gccccccagg aaatgaagga aaacgaggcc atggccttca agggcgattg
     2041 cctcaagtgc ggcctctgcg taaaagcctg cccgaaacag gcccttcgct ttccgggata
     2101 aggacggttc aagggttcaa aagttcaaga gttctaaggg taaaagacaa aaaaagcacc
     2161 gcaggccttg ggttagtgtg cggatcgtct tgacagcaca atcactcagc ggggcacgtc
     2221 atggttcttc acttgaaccc ttgaactttt gaacccttga accttctttg aggcccttgg
     2281 gccagccctg tttccattca ttgacaaagc ctcccaattc ctgtaagatt tgtggtaatt
     2341 tattaaattt tctgtgtatt tggatgaata ccgtacgcga tctcaagtca gaacactgcc
     2401 ggggagattc cagggtagag gtcaacaacc gcaagtgccg cagatgcttt tactgtgtgc
     2461 agctctgtcc cgcgaaggcc atcaaactcg aaaaggagtc gatccgcatc atcccggagc
     2521 gatgcatact ctgcggcaac tgcatcaccg gatgtccgca ccgtgcgatg acatatcaca
     2581 gcgacgcgag catcgtcagg aacctcatag aggcgaatga gaatgtcgtc gcctgcctcg
     2641 accccgcctt tccggccatt ctcgacaatg tgagcccctg gggtttcagt tacgccctca
     2701 aggccctcgg gttcaaggag gtctgggaag gtgccgtcgg tgcggagctg ataagcggac
     2761 cttaccggaa acttctgacg gaaacggaaa aacccgtcat atcttccttc tgccccgtca
     2821 tcgtctccta catcgagaag tatcttcccc agcttatcgg taatctcgcg cccatagtct
     2881 cgccgatgat agctgctggg agggccatca agaaggcgaa aggggagggg tggcgcgttg
     2941 tctacatcgc cccgtgcctt gcccggatgg gggagttgga ctacgaggag aacgcggatg
     3001 ccatcgatca tgtcatcacc tttcgcgacg tgcggttcat gttcgccgag gccgggatca
     3061 acccgagaca cgtggacgag gcgcccttcg acgggcccgt tccggcactg ggccggatcg
     3121 tccccgtcat aggcgggctc aaccggagcc tggggcacag tttcgacgtt ctcgtcgggg
     3181 agaccagcgt tgcctacggg cgaaggcggg tcatggccgc gctcaaccag cttgcgaagg
     3241 gggccattca ggcccgtttc ttcgatcttg ttttctgcga tggctgcgtg gacggttcct
     3301 tcgtggagcg agagctttcc gtggtgggaa gaaggcagat cgtatcgcgg tacgccaagt
     3361 cccagttcag cccccagacg gcgtcggtgc ttcagcgtac cctcagggac tttgctgatg
     3421 tgaagatcac gaaggagcac aaggccaacg ttcagaagct ccccgatccg acggaggagc
     3481 agatacgggc cgtcctgaag aggataaaca agctgcctcc ccacaacaac ctcgactgcc
     3541 gggcctgcgg ctacaatacg tgccgagaga aggcggttgc cgtcgtgcag ggcatatcgg
     3601 aagccgagta ttgcctgccc taccttctgg aaggctcgaa gcgcgtctac gagcagctcg
     3661 aaaaatccca cagggagctg cagcgctccc atcaggaact ggagcaggcg cagttccagc
     3721 tcatcaggac ggagaagctc gcggccctgg gacagctcgc cgccggcgtc gcccacgaga
     3781 taaacaacc
//
LOCUS       DAOPOA010000137         3757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_26582_length_3757_cov_2.950027, whole genome shotgun sequence.
ACCESSION   DAOPOA010000137 DAOPOA010000000
VERSION     DAOPOA010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26582_length_3757_cov_2.950027"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PKJ17_03980"
     CDS             complement(<1..801)
                     /locus_tag="PKJ17_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOD75160.1"
                     /translation="MSFKNMKIGIRLGIGFGIMMVLLAVLTFMGIRSMHSINAGMETI
                     VKESNVKIKCARDAMKAIDKTAEGILLTASMNDAGIRAEAKKDIEEGRNAYKENVGLL
                     EKIDTSQKGKQLIEKAKAEIKPAAEANNKTLQLAAANKFQEAVQSYVQGRPITERVKD
                     SFAELAKYQEEQAMARYNEAAATYNSTRTMLFIIGLVAVALGIVVAYLMTRSITKPLG
                     EAVHISDALAKGDLTMKVETIFRDETGQLLTAMKNMVEKLREVVEGVSS"
     gene            complement(1065..2582)
                     /locus_tag="PKJ17_03985"
     CDS             complement(1065..2582)
                     /locus_tag="PKJ17_03985"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HOD75161.1"
                     /translation="MRNTLFIMIFVLVVVGSAGADRGPVTWQENVSLSQESQKAIILH
                     NGTEEVLILGTELKASSEIDLLEFIPLPSDPEVGLAKGDPFAAVSRLISGKGLVFLAD
                     EFTTKGGPGGSSTVPVEIRFSEKVGIHDVTTVKINDIDGFSRWLEDFFRKKGIQTGRK
                     RLSGVLDTARDYMDRGYNYFVFDLVKVTERTRFIEPLMYRFRTGRIYYPLKTSNLIGG
                     KGAVEMVLVLPGSVTDDLWQGADKVFHMGAGAAIELSSSSKLNRADVQAIYPDRGFFG
                     PGSRIYLQVLRYRGQYRFKDDFIYDVGRLTPYAYRYESDTRFGAAKEFTPAFTGEERR
                     DLRDFFCPKSGDPQYIFRITDYRLDCWNFIPGEEYDVYAALFRGGLGGIPSGRVELEK
                     STTAKQLRGNRIKVDKYLAADFNGKNKVSCTLENAFPDDGLPTVRIRDGRSDEIARAQ
                     GGTFVSRVGFSRDGTLALVHVDHIAGPRSGVAYFVLLGKKGGRWEITDSFPEAIY"
     gene            2705..>3757
                     /locus_tag="PKJ17_03990"
     CDS             2705..>3757
                     /locus_tag="PKJ17_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75162.1"
                     /translation="MITVALPGGTGEITYRTGTLAVTPTEHIGKDPSTNHVPKNLRGV
                     ILAFVLSSLLVLASLFLSPPSFAQTVDDYDEAETDFEESIEFESPSGARDIIDFDIFE
                     DIARARARLEKMSCGFTVVRSKTVKKVRTGKKGKKWKQVVRYGKPELGSFTILLAVEN
                     VKTRDIRVIRVHPRLGGRTESAIIEPGKANGVNTKFTIISPEHHVVLALKRPVRHGTT
                     FREVVYTPYSEGLDIPSVRKAGLEYLQGVIDNAKNDLIARRVRPASCNSFIDNDISVV
                     LAIIEHIDPQKFTSGRYTPEKLINETLVILGTNKHNAYRYSKSKAGAVGLFQFIPATY
                     KRVQNLYPQAGLIRDFS"
BASE COUNT          764 a         1163 c          971 g          859 t
ORIGIN      
        1 ggagctgaca ccctccacga cctcgcggag cttctccacc atgttcttca tggccgtgag
       61 cagttgaccc gtctcgtccc tgaagattgt ctccaccttc atggtcaggt ctccttttgc
      121 gagcgcgtcg gagatatgca cggcttcacc aagaggtttg gtgatgcttc tcgtcatgag
      181 ataggccact acgatgccga gggcgaccgc cacgagccct atgatgaaga gcatcgtcct
      241 cgtcgagttg tacgttgccg cggcctcatt gtaccgtgcc atcgcctgct cttcctggta
      301 cttcgcaagc tcggcaaagg agtccttgac cctttcggtg ataggccgcc cctgtacata
      361 tgactgcacg gcctcctgga acttgttcgc cgccgccagc tgcagggtct tgttgttcgc
      421 ctccgcggcg ggctttatct ccgctttggc cttctctatg agttgctttc ctttttgtga
      481 tgtatctatc ttttcgagga gccccacatt ctccttatag gcgttgcgcc cctcctcgat
      541 gtccttcttt gcctccgccc ggataccggc atcattcatt gacgccgtga gcagtatccc
      601 ctcggcggtc ttgtcgatcg ccttcatggc atcgcgggcg cacttgatct tcacgttgct
      661 ctccttgacg atcgtttcca tgcccgcgtt tatggaatgc atgctcctta tccccatgaa
      721 cgttaggacg gcaagcaaaa ccatcatgat accgaatccg attcccagcc gtataccgat
      781 cttcatgttc ttgaaactca tctgtgatcc ctccttgtgt tgtgcgtgaa ctttgagtct
      841 ctcttttcag gaagtgctgt ttccatggcc tcgtgtccgc gggactgatg acgaacgatc
      901 tccgcaggcg tctttcattc gttcgatgcg ttgcggcgat cgtatcctct tttgtgtaat
      961 cgcaggatat gccagatcgc ttaaaccaaa aagcacaaga gattttcaac ctgccgaaat
     1021 gacagggaat tgtgatatca atgggatatg gggtgttgcg ggtgtcagta gatggcttcc
     1081 ggaaaggagt cggtgatctc ccatctgccc cctttctttc cgagcaaaac gaaatacgcc
     1141 actcccgacc ggggccccgc gatatggtcc acgtgcacca gcgccagggt cccgtctctc
     1201 gaaaaaccca cgcgggagac aaaggtcccc ccctgagcgc gggctatctc atcgctcctg
     1261 ccatctctta tcctgaccgt aggcaggccg tcgtcgggga aagcgttctc gagggtgcag
     1321 cttaccttgt tcttgccgtt gaaatccgcc gcaagatact tgtcgacctt gatcctgttg
     1381 ccccggagct gctttgcggt ggtgctcttc tcaagctcga cccggcccga tgggatgccg
     1441 cccaatcccc cgcggaacag cgcggcatac acgtcatatt cctcgccggg aatgaagttc
     1501 cagcagtcaa gccggtagtc ggtgatccgg aagatgtact gcgggtcgcc gctcttcgga
     1561 cagaaaaaat ccctgaggtc ccgcctctct tcccccgtga aggcaggggt gaactccttc
     1621 gccgcgccaa accgggtgtc gctctcgtag cggtacgcgt agggcgtcag tctcccgacg
     1681 tcgtagatga agtcgtcctt gaatctgtac tggcccctgt acctcagcac ctgcaggtag
     1741 atcctggacc ccggtccgaa gaaaccccgg tcggggtaga tggcctgcac atccgcccga
     1801 ttgagtttcg atgatgagct gagctcgatc gctgctccgg cacccatatg gaaaaccttg
     1861 tccgcaccct gccacaggtc gtccgtcacg gacccgggca acacgaggac catctccacg
     1921 gcgcccttcc ctcctatgag gttggacgtc ttaaggggat aatagatcct gcccgtcctg
     1981 aaccggtaca tcagcggctc gatgaagcgc gtcctctcgg ttaccttgac gagatcgaaa
     2041 acgaaatagt tgtatcccct gtccatgtag tcccgcgcgg tgtcaagcac gcccgacagt
     2101 ctcttcctgc cggtttggat accctttttc ctgaaaaagt cctcgagcca tcgggaaaac
     2161 ccgtcgatat cgttgatctt caccgtggtg acgtcatgga tcccaacctt ctccgaaaag
     2221 cggatctcga cggggaccgt cgaggaaccc cctggcccgc ccttcgtcgt gaactcgtcc
     2281 gctagaaaga cgagaccctt tccagagatc agccgtgaaa ctgccgcgaa gggatccccc
     2341 ttcgcaagtc ctacctcggg atcggaaggc agaggtatga actccaggag atcgatctcc
     2401 gagcttgctt tcaactccgt cccgaggatc agcacctctt ccgtgccgtt atggaggatg
     2461 atcgcctttt gggattcctg gctcaaactg acgttctcct gccacgtcac ggggcccctg
     2521 tcggcaccgg ctgaacccac gaccacaagc acaaagatca ttatgaacag ggtattcctc
     2581 atcgcctcac tccttcagcc tcgttacagg ccgcattctc cagcattctc cagattcaac
     2641 accatccacc cgcgcgatgt ccacactttc tttaatactt ttgttcctcc ggtcaggatg
     2701 tgctatgatt acagtcgcac tccccggcgg caccggggag atcacctatc ggacaggtac
     2761 attggcggtt acaccaacgg agcacatagg caaagaccct tcgacgaacc acgtcccgaa
     2821 gaaccttcgc ggcgtcatcc tcgctttcgt cctctccagt cttcttgtcc tcgcttctct
     2881 tttcctttct cctccatcct ttgcccaaac cgtggatgac tatgacgaag cggagaccga
     2941 tttcgaggaa tccatcgagt tcgagtcccc atcgggagcg cgtgatatca tagatttcga
     3001 catcttcgaa gatatcgccc gggcaagggc acgccttgaa aagatgagct gcggcttcac
     3061 tgtcgtccgg tcgaagaccg tgaagaaggt caggacgggc aagaaaggaa agaaatggaa
     3121 acaggtggtg cgttacggaa agcccgaact gggatccttc accatccttc tggccgtcga
     3181 gaacgtgaag acaagggaca tacgggtcat aagggtccac cccaggctgg gcggccgcac
     3241 tgaaagcgcc atcatcgagc cgggcaaggc gaacggggtc aacacgaaat tcaccatcat
     3301 ctcccccgag catcacgtcg tgctcgccct caagaggccc gttcgtcacg ggacgacgtt
     3361 cagggaggtc gtatacaccc cctattcgga aggccttgat atcccctccg ttcgaaaggc
     3421 aggattggag taccttcagg gcgtgatcga caatgcaaag aatgacctca tcgccagacg
     3481 ggtgaggccc gcttcctgca acagcttcat cgacaacgac atttccgtgg tgcttgccat
     3541 catagagcat atcgatcccc agaagttcac gagcgggcgg tacacgccgg aaaagctcat
     3601 caacgagacg ctcgtcatac tggggacgaa caagcacaat gcctaccggt actcaaagtc
     3661 gaaggcgggg gcggtgggtc tcttccagtt catcccggca acctacaaga gggtccagaa
     3721 cctgtacccg caggcgggcc tcatacggga cttctcc
//
LOCUS       DAOPOA010000138         3731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_26801_length_3731_cov_3.388466, whole genome shotgun sequence.
ACCESSION   DAOPOA010000138 DAOPOA010000000
VERSION     DAOPOA010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26801_length_3731_cov_3.388466"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..620)
                     /locus_tag="PKJ17_03995"
     CDS             complement(105..620)
                     /locus_tag="PKJ17_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOD75163.1"
                     /translation="MVIFMTSCLKVLSMLMIFTMLGAIGFATGDLTADKLLVEKKARR
                     LTLLSKGQILRTYKIALGGNPEGPKEREGDGKTPEGSYIIDGRNTRSGYHLSLHISYP
                     NEKDRKRAKELGVSPGAQIMIHGMKNGLGWIGPFHTLYNWTQGCIAVTDAEIDEIAPL
                     VPNGTPIEIRP"
     gene            1105..1794
                     /locus_tag="PKJ17_04000"
     CDS             1105..1794
                     /locus_tag="PKJ17_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOD75164.1"
                     /translation="MMMTTKVSRQRLSNQVYDILKEMIADYRFSPGARINVEQIAKEV
                     GASRTPVWEAVHRLIQEGLLENIPNRGVFMVALRPEKAIELYEVRNALEGLAAGLAAV
                     NIDAKTLKKMKRSLEEQMKVVEAGDLVAYSQLDYEFHALVYEASRNKTLKEMLEEVKS
                     KMRPIGMHITPVLADLYQDHVDLLDSLERHDAHGAEVAFTRHNSRMIELIEKSVGSDT
                     WKGAHENTDKK"
     gene            1841..3091
                     /locus_tag="PKJ17_04005"
     CDS             1841..3091
                     /locus_tag="PKJ17_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD75165.1"
                     /translation="MKIKVFAVVVLTVLFAAGSLCVIPDASAQDKVRVGLIFGLTGAA
                     SPIGPLQLDGAKLAIKEINAAGGVKLGGKKVPIEFYVKDDESKPDVAIRRFRELQDEN
                     KVNVVVGQTFAPISAALNKEAKKTSIAYFPVNVVAVGMFEKKEMAETTFAIHGSAYSI
                     GYAGASYIVDKLGLKNIIFFGPAYAFGRDQWQGAKDAFEARKVKVEYLESPVGTSDYT
                     SYLLKIKEKKPQIVMLAHWGVDAINVLKQTYEVGIKKNSKVWFNWMTNAFGSGVPPEA
                     LEGVYSLMGWYYDMKGFQDAAIVKASDAFAKKFQAEYKYPPDPYSAMAYEGVREAIRG
                     IELANSLEGKAISKAIMANPTFDSMKGKGTWRADHQPLFKYGAYVTIGKGSKERKNAK
                     WDLVKIIGAYTGEDYLPNLKALGY"
     gene            3162..>3731
                     /locus_tag="PKJ17_04010"
     CDS             3162..>3731
                     /locus_tag="PKJ17_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD75166.1"
                     /translation="MSANPIIETKKATIRFNGLTAVDTVDFRMEAGEAVGVIGPNGAG
                     KTTFFNLLTGLYTPTEGAILFSGKDITKVPPHRRVALGIIRTFQLVSVFDSLPVIDNL
                     ALSTIRFGENYGGKGKFFFANVHSQRIMKACMEKLEVLGISDKADLMTSELSYGDKRK
                     LEIAVALSLNPKILLLDEPFAGLSDSEIGE"
BASE COUNT          852 a         1062 c         1104 g          713 t
ORIGIN      
        1 tagagaaacc tctggattta aggccttcgc cggaatgacg tagaaaatgg gtttaccaac
       61 agcctgtcaa gcggtttaag cggctcaagc ggttcaagcc gtctttatgg ccgtatctcg
      121 atgggcgtgc cgttggggac gaggggggcg atctcgtcta tctccgcgtc ggtgacggcg
      181 atgcagccct gggtccagtt gtagagggtg tggaaggggc caatccatcc gagaccgttc
      241 ttcatgccgt ggatcatgat ctgtgcgccg ggggaaacgc caagctcctt cgcgcgcttc
      301 ctgtccttct cgtttgggta ggagatgtgg agggacaggt ggtacccgct gcgggtgttc
      361 cgcccgtcga tgatgtagct cccttcaggc gtcttcccgt cgccctccct ttccttcggc
      421 ccctcggggt tcccgccgag ggctatctta tacgtcctca ggatttggcc tttcgataac
      481 agcgtcaggc gcctcgcctt tttctcgacc aggagcttgt cggccgtgag gtccccggta
      541 gcgaacccga tggcccccag catggtgaag atcatcagca tggacaggac tttgaggcag
      601 ctcgtcatga agatgaccat accatttcct ggggaccttt accagcgacc gggtggggaa
      661 agactttatc tcacgcccgc ttcggtagat agcgcagaga aaggcaaatg agagtgtgcg
      721 gaaaaagatt gcggcagatc gcggagcctt gtacgatctg accgccttcg catcccgtct
      781 gcgacgggag ggaaaacagg accgcgggga tggaattata ggacctctcg gacatatagg
      841 acttataaga cctatggcag cagcagaaaa cgggaaaccc ctttcccagg ataagaaaac
      901 gatattctct tctcatcctc tgcgccctcg agcgaagcgg gcgtgagata acgcctcggc
      961 ctttgacccc catattcgtt cctgtcaaaa tagcctctta gtcttttgtt tcagtacctt
     1021 gtcatactag caatttcatg aaatatgaaa aatatttcgt gaaagagctt gacaggattc
     1081 ccatgcgggg gatagaatta ccctatgatg atgacaacga aggtttccag gcaacggctc
     1141 agcaaccagg tctatgacat tttgaaggag atgatcgcgg actaccgatt cagtcccgga
     1201 gccaggatca acgtggagca gattgccaag gaggtgggcg cctccaggac ccctgtctgg
     1261 gaggccgtgc accggctgat ccaggaaggg ctcctcgaga acatccccaa caggggtgtc
     1321 ttcatggtcg ccctgaggcc cgagaaggcc atcgagctct acgaggtcag gaacgcgttg
     1381 gagggcctcg ccgccggcct cgccgccgtg aatatcgacg cgaagacgct gaaaaagatg
     1441 aagaggtccc tcgaggaaca gatgaaggtc gtagaggcgg gagaccttgt agcctactcc
     1501 cagctcgact acgaattcca cgccctcgtc tacgaggcaa gccggaacaa gacgcttaag
     1561 gagatgcttg aggaggtgaa gagcaagatg cggcccatcg gcatgcacat cacccccgtc
     1621 ctggcggacc tctaccagga ccacgtggat ctcctcgact ccctggagag gcacgacgcg
     1681 cacggcgccg aggtggcctt caccaggcac aactcccgca tgatcgagct catcgagaaa
     1741 agcgtcggct ccgacacctg gaagggagcc cacgagaata cggacaagaa atgatagtcg
     1801 aagggatctt cacatcatga aaggaggaat cgcaatgtcc atgaagatca aggtttttgc
     1861 agtcgttgtt ctgacggtac ttttcgcggc ggggtcgctg tgcgtgatac ccgacgcctc
     1921 cgcgcaggac aaggtaaggg tcggtctcat attcggactc accggagcgg catcacccat
     1981 cggccccctg cagcttgacg gggccaagct ggcgataaag gagatcaacg cggcgggcgg
     2041 cgtgaaactg ggcggcaaga aggtccccat cgaattctac gtcaaggatg acgagtcgaa
     2101 gcccgacgtc gccatcaggc gcttcaggga actccaggat gagaacaagg tgaacgtcgt
     2161 cgtcggccag accttcgcgc ccatctccgc ggccctcaac aaggaggcca agaagacctc
     2221 catcgcctat ttccccgtga acgtggtggc tgtcggcatg ttcgagaaga aggagatggc
     2281 ggagacgacc ttcgcgatcc acggcagcgc ctattcgatc ggctacgcgg gagcctccta
     2341 catcgtcgac aagctgggtc tcaagaacat catcttcttc gggcctgcct acgcctttgg
     2401 ccgcgaccag tggcagggtg cgaaggacgc attcgaggcc cgcaaggtca aggtggagta
     2461 cctggaatca cctgtgggaa cgagcgacta cacgtcatac ctcctgaaga tcaaggaaaa
     2521 gaaaccgcag atcgtcatgc tcgcccactg gggtgtcgac gcgatcaacg tcctcaagca
     2581 gacatacgag gtcggcatca agaagaactc caaggtgtgg ttcaattgga tgacgaacgc
     2641 cttcggaagc ggcgtaccgc cggaagcgct ggagggcgtc tactccctca tgggctggta
     2701 ctatgacatg aagggtttcc aggacgcggc gatcgtcaag gcgtccgacg ccttcgcgaa
     2761 gaaattccag gcggaataca agtatccccc cgatccctat tcggcaatgg cctacgaggg
     2821 cgtaagggaa gccatccgcg gcatcgagct tgccaattca ctcgagggca aagcgatatc
     2881 gaaggccatc atggccaacc ccaccttcga ttccatgaag ggaaagggaa cgtggcgggc
     2941 cgaccatcag ccgctgttca agtacggtgc ctatgtcacc atcggcaagg ggtccaagga
     3001 gcggaagaat gccaagtggg acctcgtgaa gatcatcggg gcctatacgg gcgaggatta
     3061 ccttccgaac ctgaaggctc tcggatacta gcataggaca cgacggacgg ggcggatacg
     3121 cgtccgcccc gtccccgaga acagcacgac gggagtttca gatgtcagcg aacccgataa
     3181 tcgagacgaa aaaggcgact atacgcttca acggactcac ggcggtggat actgtcgatt
     3241 tccggatgga agccggagag gcggtgggcg tcatcggccc caacggcgcg gggaaaacga
     3301 cgttcttcaa cctcctcacg ggcctgtata ccccgacgga gggcgccatc ctcttctcgg
     3361 gcaaggatat aacaaaagtc ccccctcaca ggagggtggc cctggggatc atacggacct
     3421 ttcagctcgt ctccgtcttc gattccctcc ccgttatcga caatttggct ctttccacca
     3481 tccgtttcgg cgagaactac ggggggaagg gcaagttctt cttcgccaac gtccacagtc
     3541 agcgcatcat gaaggcctgc atggagaagc tcgaggtcct gggcatctcc gacaaggcgg
     3601 acctcatgac atcggagctt tcctacgggg acaagcgcaa gctcgagatc gccgtcgcgc
     3661 tgtccctcaa cccgaagata ctccttctcg acgagccctt cgcgggcctc tccgacagcg
     3721 agatcggcga g
//
LOCUS       DAOPOA010000139         3728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_26829_length_3728_cov_2.877212, whole genome shotgun sequence.
ACCESSION   DAOPOA010000139 DAOPOA010000000
VERSION     DAOPOA010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26829_length_3728_cov_2.877212"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3574
                     /locus_tag="PKJ17_04015"
     CDS             <1..3574
                     /locus_tag="PKJ17_04015"
                     /inference="COORDINATES: protein motif:HMM:NF025128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SwmB domain-containing protein"
                     /protein_id="HOD75167.1"
                     /translation="RKEYGPNETVRICSTIENPSDADFSIVVNAQLADARGHIVDVAA
                     HETGPAVKLGAGASESVTLLWNTGMSQPGAYSVRFHVADAASGSVVDDRKLAFVVTPS
                     VVVDDLDLVVSPSNLNQDEKRTVDMCLTLQNRSNTDAFLTAELLVKDPEGAVVHEGRV
                     DLELPLTTAEGVLELPPLTHVFDRCGQYPVEVSVRSGEVLCGQVRTTIRVAAATRIDV
                     VKTVDPATVTPDGDKRVRVAIRLEGVGATVNPSFLSAGTNTGGDRITIAFDKAMAEPG
                     GGVPLFTVTADGAPCPVTEVLIGESDITRIDLVLAAAVARGQEILVSYAGSILTCVEG
                     KPLMPFDDEAVANRVSPPIFNQDGYGFSGSIAPNPLAPRTVMTGYGQWPRGFYKNVLS
                     FAGAVFDGQAIWMVPANADSVVRVDTGTGEMTAFNRWPEGFSKGGHAFAGGIFDGECL
                     WMIPSYADSVVKLDTGTGEMTRYNAWPKGYAKGGYAFAGGIYDGRHIWMVPANADSVV
                     IVDTRTGEMSAYSGWPEGLGKVEYAFAGGIYDGRHIWLVPYYADRVVKVDCQTGEMTG
                     HHRRPEGLGKVEYAFAGGVFDGQGIWMIPLNADRVIRIDKDTAEVTEYAEWPQGFMKG
                     VNAFAGGVFDGESIWMVPSYADRVVRISSFSSLSLSAAITASDSFSAYISQDESGEGR
                     LAGRGKGWSSVHTLNAALVPGLTNYLHVKCVDTAGPVAAFMADLVLNDESFHFADGSR
                     HLVTSESGWRVYTDRFGGTEGSVTAVCRNGEGSWSTRFGIDLDANWIWTGRGMDKGAR
                     FFSIPIYYSPVPVAPMEDVVIIDTIAGGKVAVDEGSFTREPSRVEQGGDGTVIEWRLR
                     EFRVGQREDIYFNVMLRDTTPGERRLVNHSLDVAYKDASGAMARRELGPCHVLVLSSP
                     FDCSIGTERQVYRMGEDVVIFCFIRNLSDLPRTLDVRVMMEDAQGVLIDQTLIRDVRC
                     APGEDMGIDGLVFKLVATNAGPHRLRLLHHEGEEETDLAEAGFMVEDVPATGIPTQGV
                     SVADVEERAGRVGAVNVSEESGADECGRAEGVLGPVPEGLPEGALEGLEGTISAQPSP
                     IFQGLSETIYYTVTQGDGGDLTGLSVNVVIADGETGKARQSFSAPRAHFKGRSFAGSF
                     TFSTGALEPGEYRVKLMIPPGSGSEAREIATTSFVIRRIDMVVT"
BASE COUNT          711 a         1020 c         1294 g          703 t
ORIGIN      
        1 cagaaaggag tacggtccga acgagacggt gcgcatatgc tccaccatcg agaacccgtc
       61 ggacgcggac ttcagcatcg ttgtcaacgc tcagctggcg gatgcccgcg gccatatagt
      121 ggatgtggcg gcccacgaga ccggtcccgc ggtgaaactc ggcgcagggg cctccgagtc
      181 cgtgacgctc ctgtggaaca ccggcatgag ccagcccggc gcctacagtg tgcgtttcca
      241 tgtcgctgac gctgccagcg ggagcgtcgt cgacgacagg aagctagcat ttgtcgtcac
      301 cccttctgtc gtcgtcgacg atctcgatct ggttgtgtcc ccttcgaatc tcaaccagga
      361 tgaaaagaga acggtggaca tgtgtctcac gttgcagaac aggtccaata cggacgcatt
      421 tttgaccgcg gagctgctgg tgaaggaccc ggagggagcg gtggtccatg aaggacgggt
      481 cgatcttgaa ctgccgttga cgacggccga gggggttctg gagctgcctc ccctgaccca
      541 tgtctttgac cgctgcgggc aatacccggt ggaggtgtcg gtccgttcgg gagaggtcct
      601 atgcggacag gtgaggacca ccatacgggt cgccgcggcg acgaggatcg acgtcgtgaa
      661 gaccgtggat cccgcgacgg tcacaccgga cggggacaag cgggtgcggg tggccatccg
      721 gctggaaggt gtcggtgcga cggtcaaccc ctccttcctg tccgccggca cgaatacggg
      781 cggcgacagg ataactatcg ccttcgataa ggccatggcg gagccgggcg gtggggtccc
      841 attgttcacg gtcaccgccg atggagcgcc ctgtcccgtc acggaggtgc ttatcgggga
      901 atcggacatt accaggatcg accttgttct cgccgcggcg gtggcacggg ggcaggagat
      961 cctggtgagc tatgcgggct cgatccttac gtgcgtggaa ggcaagcccc tcatgccttt
     1021 tgacgatgaa gcggtcgcga acagggtgtc gccgcccata ttcaaccagg acggatacgg
     1081 tttcagcggc tccatcgcgc cgaacccgct ggccccgagg accgtcatga ccggatacgg
     1141 ccagtggccc cgggggtttt ataagaacgt gctctccttt gccggcgccg tcttcgatgg
     1201 acaggcgatc tggatggtcc cggcgaacgc ggacagcgtc gtgagagtgg acaccgggac
     1261 gggggagatg actgccttca acaggtggcc ggaggggttc tcgaaaggcg gccacgcctt
     1321 cgcggggggc atcttcgacg gcgagtgcct gtggatgatc ccctcctacg cggacagcgt
     1381 cgtgaaactg gataccggca ctggcgagat gacgaggtac aacgcgtggc cgaaggggta
     1441 tgcaaagggt gggtacgcct tcgcgggggg catctatgac ggccgtcaca tctggatggt
     1501 cccggcgaac gccgacagcg tcgtgatagt ggacacgcgc acgggcgaaa tgagcgcgta
     1561 cagcgggtgg cccgagggac tcgggaaggt cgagtacgcc ttcgcggggg gcatctatga
     1621 cggccgtcac atatggctcg tgccctacta cgcggaccgg gtcgtaaagg tggactgtca
     1681 gacgggtgag atgaccggtc atcaccgcag gcccgaggga ctcgggaagg tcgagtacgc
     1741 ctttgcgggc ggcgtcttcg acggtcaggg tatctggatg atccccttga acgccgacag
     1801 ggtcatcagg atagacaagg atacggcaga ggtgacggag tacgcggaat ggccccaggg
     1861 cttcatgaag ggggtgaatg cctttgcggg cggcgtcttc gacggtgaat ccatctggat
     1921 ggttccctcg tacgcggaca gggtggttcg catctcctcc ttttcgtcgc tatccctttc
     1981 ggcggccatc accgcgagtg attccttctc agcatacata agtcaggacg agtccgggga
     2041 aggcaggctg gcaggccggg gaaagggatg gtcctcagtt cacaccctca acgctgcgct
     2101 cgttccaggt ctcaccaact acctgcatgt gaagtgcgtc gataccgcgg gcccggtggc
     2161 ggcattcatg gccgacctcg tcctcaacga tgagagcttt cactttgcgg acgggtccag
     2221 gcaccttgtg acctcggaga gtggctggcg ggtctacacg gacaggtttg ggggcaccga
     2281 aggttctgtt acggctgttt gcaggaacgg cgaaggctcg tggtcgaccc gtttcggcat
     2341 cgatctcgac gcgaactgga tatggacggg cagaggcatg gacaaggggg cacggttctt
     2401 ctccatcccg atatactatt cgcccgttcc cgtcgctccc atggaggatg tggtcatcat
     2461 cgacacgata gcaggcggga aggtggcagt ggatgaaggt tcctttacgc gcgagccttc
     2521 ccgggtcgaa cagggagggg acggaacggt cattgagtgg cgcctcaggg aattcagggt
     2581 gggtcagcgg gaggatatct attttaacgt tatgctgagg gacacgaccc ccggcgagag
     2641 gcgtctcgtc aaccattccc tcgatgtggc ctacaaggat gcgtcagggg cgatggcgcg
     2701 cagggagttg ggtccctgtc acgttcttgt cctttcctct ccctttgact gctccatagg
     2761 aacggagcgg caggtctaca ggatgggaga ggacgtggtc atcttctgct tcatcaggaa
     2821 tttgagtgat cttcccagga ccctcgacgt cagggtgatg atggaggatg cccagggggt
     2881 gctcatcgat cagaccctca tacgggatgt gcggtgcgcg ccgggtgagg acatgggtat
     2941 tgacgggctt gtcttcaagc ttgtcgcgac gaatgccggg ccccacaggc tccgcctgct
     3001 gcaccatgaa ggcgaggagg agacagacct ggccgaggcc ggcttcatgg ttgaggatgt
     3061 gcccgcaacg ggaattccca cccagggcgt ctccgtggcg gatgtagagg agcgtgctgg
     3121 aagggttggg gctgtgaatg tgtccgaaga gagcggcgcc gatgagtgcg gccgggcgga
     3181 gggggttctt gggcccgtgc cggaaggcct gcctgaggga gcgctggagg gtctggaggg
     3241 aacgatcagc gcccagccga gccccatatt ccaggggctg tcggagacca tttactacac
     3301 ggtgacgcag ggtgacggtg gcgacctgac gggactcagc gtgaacgtcg tgatcgcgga
     3361 cggcgaaacg ggaaaggcca gacagtcctt ctccgccccc cgggcccact tcaagggcag
     3421 gtcctttgcc ggaagcttca ccttttcgac gggcgcgctt gaacccgggg agtatagagt
     3481 gaagctgatg atcccgccgg gttccggaag cgaagcgcgg gagatcgcaa ccacgagttt
     3541 tgtgatccgc aggatagaca tggtagtgac ctgacgcggg gcggcggcac gccgtcagtg
     3601 gcagaacgag catgaaagga ggtgcttatg ccgaacgatt tcattgggga tctttcaaaa
     3661 acaaggctcc tcgatctgat atcaccgctt ttgagcagaa agaaatcggg catgatccag
     3721 atcaaggg
//
LOCUS       DAOPOA010000140         3705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_27048_length_3705_cov_3.469589, whole genome shotgun sequence.
ACCESSION   DAOPOA010000140 DAOPOA010000000
VERSION     DAOPOA010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27048_length_3705_cov_3.469589"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..361)
                     /locus_tag="PKJ17_04020"
     CDS             complement(143..361)
                     /locus_tag="PKJ17_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75168.1"
                     /translation="MKARIDRMSGTQKTRGDINESVRECFHLLAKSCERYGEMERSEA
                     PEVVMDMEKLLIWKRIMFLFNADPDRRN"
     gene            506..847
                     /locus_tag="PKJ17_04025"
     CDS             506..847
                     /locus_tag="PKJ17_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75169.1"
                     /translation="MKKQKMGTPDLRDKLVTETVKKVMTLLKSKGQEPDKAAVERTTN
                     EAIDDFVAQWYEVSLEAIELRDGEADFVTDDELDDPELIHEAKLRVIKELYEEPPEDF
                     YETIAQSAITS"
     gene            complement(844..3270)
                     /locus_tag="PKJ17_04030"
     CDS             complement(844..3270)
                     /locus_tag="PKJ17_04030"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75170.1"
                     /translation="MITKLKPDELYKKCDPDSFAFAGTDAVVELPQTIGQEKALNSLD
                     FGLNMDSAGFNIFAIGESGTGKMSTVMYMLRKKAANEEAPADWCYVHNFKDPDSSIAL
                     SFGPGRGAVFQKELEEMIKILKLEIPKAFESKEYETQKSKIVDEFQQKQQEYLARLDS
                     EAKERGFTVKRGPTGVLIVPVKENGELMSKEEFEALDAKARKKLEETGRLFQEKLNDV
                     VRILREAEKIVGEMVMRLDRMVALDIVGPMVDAIQKKYGDQEKVVKYLEDVKEDILTH
                     LDDFKATEEAPSPLPFLKIPKAETSFARYAVNVIVNNGESKGAPVVYESNPTYLNLFG
                     RMEYKVQYGMATTDFTMIKAGSLHKANGGYLVIDALELLRNAFSYEALKRALKNGEIR
                     IEDVLEQYRLISTAGLKPEAIHLNVKVVLTGNPYLYYVLHAYDEDSRELFKVKADFDS
                     RMERNRENTDKYALYVAQCQKDENLLPFDRTAVARLVEMGSRFADHQDKLSTRFGDIA
                     DLIRESHYWAKKEGMDVVSASHVTRAVKEKIYRVNRIEERLQEMMLEDTLIVNTAGEK
                     VGQVNGLAVLDIGDYAFGKPSRITAKTYMGRAGIVNLERETKMSGKIHEKAILIITSY
                     LGGKYAVNKPISLSASITFEQLYEMVEGDSATCAELYALLSSISGVPLKQSFAVTGSM
                     DQNGDVQPIGGVNQKIEGFFHLCKMRGLDGSHGVMIPKRNARNLVLSDDVIEAVEKGL
                     FSIYTIDRMEEGLELLTGMPAGELGADGSYPEGTLNRLVEKRLTEISEAMEKKKGNGE
                     EDREKKDGYY"
     gene            complement(3476..>3705)
                     /locus_tag="PKJ17_04035"
     CDS             complement(3476..>3705)
                     /locus_tag="PKJ17_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOD75171.1"
                     /translation="PHRITANTIAPSFIFNEFIPHIYPQDEIDRMFDEIPYPRKGTPE
                     DVANAALFLASDEGEYMTGQTLCVTGGSWMR"
BASE COUNT          740 a         1086 c          996 g          883 t
ORIGIN      
        1 aagcatcgcc tattgtaacg tgaaggcgcc tgcaaaagca acgatgcaac tgcagcaggc
       61 tttggtccgg cgcatctctc gagggcacag accgcgcgac ggcgatacct cctgccggtc
      121 atcccgtgtc agtattctga catcagtttc ggcggtcggg atccgcgttg aagaggaaca
      181 tgatccgttt ccagatgagc agcttttcca tgtccatgac gacttccggc gcctcggacc
      241 tttccatctc gccgtatctc tcgcagctct tggcgagaag gtgaaagcat tcccggacgg
      301 attcgttgat gtccccacgg gtcttttgtg ttcctgacat cctgtcaatt cgagctttca
      361 tgccagagac ttctgcaagc tctgtgccaa accacctcca caaaaactat tttactccgc
      421 gccgtgtttt gtgtaagaat gaatgaaagc agccacgatt acggatacaa cgacccatgg
      481 ggcgtctctt cggagggttt gcgcgatgaa aaaacagaag atgggaaccc cggacctgcg
      541 ggacaaactc gtgaccgaga ccgtcaagaa ggtgatgacc cttctcaaga gcaagggaca
      601 ggaaccggac aaagccgcag tcgaaagaac gacgaacgag gccatagatg atttcgtcgc
      661 acagtggtac gaggtttcac tcgaggccat cgaactcagg gacggcgagg ccgatttcgt
      721 taccgatgac gagcttgacg accccgaact catccatgag gcgaagctca gggtcataaa
      781 agaactctac gaagaacccc ccgaagattt ctacgagacc atagcacaga gtgccattac
      841 ttcctaataa tagccgtctt tcttctcgcg gtcttcctct ccgttgcctt tctttttctc
      901 catcgcttcc gagatctccg taaggcgctt ttccacgaga cggttgaggg tgccctcagg
      961 ataactcccg tcggccccga gttcacctgc cggcatgccg gtcaggagtt caagcccctc
     1021 ctccatgcgg tcaatcgtgt aaatggagaa aaggcctttc tccacggcct cgatcacgtc
     1081 gtcgctcagc accaggttcc gtgcgttgcg tttcgggatc atcactccat gtgatccgtc
     1141 gagaccacgc atcttgcaga ggtggaagaa gccctctatc ttctggttca ccccgcctat
     1201 gggttgcacg tccccgttct ggtccatgga gcctgtcacg gcgaaggact gcttcagcgg
     1261 aacgccgctg atggatgaaa gcagcgcgta cagctcggca caggtagcgc tgtcaccttc
     1321 gaccatctcg tagagctgct cgaaggtgat cgaagcggag aggcttatgg gcttattgac
     1381 cgcgtatttg ccgccgaggt agctcgtgat gatgaggatg gccttctcgt ggatcttgcc
     1441 gctcatcttc gtctcgcgct ccaggttgac gatcccggcc ctgcccatgt aggtctttgc
     1501 ggtgatacgg gatggtttgc cgaaggcgta gtcgccgata tcaaggacgg cgaggccatt
     1561 cacctgtccc accttctcgc ctgcggtgtt cacgataaga gtatcctcaa gcatcatctc
     1621 ctgcagtctt tcctcgatgc ggttcaccct gtagatcttc tccttgacgg cccgcgtcac
     1681 gtgcgaggcg ctcacgacat ccatcccttc cttctttgcc cagtaatgag attcccgtat
     1741 gaggtcggca atgtcgccga agcgtgtcga gagcttatcc tggtggtcgg caaaacgcga
     1801 gcccatctcc acgagccgcg ccacagccgt cctgtcgaag gggagaaggt tctcatcttt
     1861 ctgacactgg gctacgtaca gcgcgtactt gtccgtgttt tcccggttcc tttccatccg
     1921 gctgtcgaaa tcggccttca ccttgaaaag ctcccgggag tcctcgtcgt aggcatggag
     1981 aacgtagtag agataggggt tgcccgtcag gacgaccttc acattgaggt ggatcgcctc
     2041 gggcttcaaa cctgccgtgc tgatgaggcg gtattgctcc agaacgtcct cgatgcggat
     2101 ctccccgttc ttgagggccc gcttcaatgc ctcgtaagag aaggcgtttc tcaggagttc
     2161 aagggcgtcg atgacgagat agcccccgtt cgccttgtgc agtgagccgg cctttatcat
     2221 cgtgaagtcc gtcgtcgcca tgccgtactg tactttgtac tccatcctgc cgaagaggtt
     2281 gagataggtc gggttgctct cgtacaccac gggggctccc ttgctctccc cgttgttgac
     2341 tatgacattg acggcatacc tggcgaaaga ggtctccgcc ttcgggatct tgaggaaggg
     2401 cagcggcgaa ggagcctcct cggtggcctt gaaatcatcg agatgagtca atatgtcctc
     2461 tttcacgtcc tcgaggtact tgacgacctt ctcctggtcc ccgtacttct tctggatggc
     2521 atcgaccatg gggccgacga tatcgagggc gaccatcctg tcaaggcgca tcaccatttc
     2581 gccgacgatc ttctccgcct ccctgaggat acggaccaca tcgttcagtt tctcctggaa
     2641 gagacgcccc gtttcttcga gcttcttcct cgccttggcg tcgagggcct cgaactcctc
     2701 cttcgacatc agttccccgt tctccttgac gggaacgatg aggacccccg tcggcccccg
     2761 ttttaccgta aagcccctct cctttgcctc gctgtcgaga cgggccaaat actcctgctg
     2821 tttctgctgg aactcgtcga cgatcttgct cttctgcgtc tcgtattcct ttgattcaaa
     2881 ggccttcggt atctcgagct tgaggatctt gatcatctcc tcgagctcct tctggaacac
     2941 ggcacccctg cccggcccga aggaaagggc tatggagctg tcggggtcct tgaagttgtg
     3001 gacgtaacac cagtcagccg gggcttcttc gttggccgcc ttcttcctga gcatgtacat
     3061 caccgtggac atcttcccgg tgccgctctc gccgatggcg aagatgttga aaccggcact
     3121 gtccatgttg agaccgaaat ccagggaatt gagggccttt tcctggccga tcgtctgcgg
     3181 caattcgacg acggcatcgg tcccggcaaa ggcaaaggaa tcgggatcgc acttcttgta
     3241 gagttcatcg ggcttcaact ttgtgatcac gggctgcctc ctgatcgtgt actgtcgaca
     3301 aaggttccgg tcctgaaacg ttacataaaa aataacacct cacagcgaat aatcaacccc
     3361 gaaccaaaaa acgttcaaga gttcagaagt tcaagcgttc aagagtggga aaaacaagcg
     3421 taccccgccc ttcagcccct tgaacctttg aacgtttgaa cccttgaacc gttttctacc
     3481 tcatccagct tccgccggtg acgcacaggg tctggcccgt catgtattct ccctcgtcgg
     3541 aggcaaggaa gagcgcggcg ttcgcgacgt cttccggggt ccccttgcgg gggtagggta
     3601 tctcgtcgaa catcctgtcg atctcatcct gtggatagat gtgcgggatg aactcgttga
     3661 agatgaaact gggcgctatg gtgttggccg tgatgcggtg ggggg
//
LOCUS       DAOPOA010000141         3656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_27517_length_3656_cov_2.827270, whole genome shotgun sequence.
ACCESSION   DAOPOA010000141 DAOPOA010000000
VERSION     DAOPOA010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27517_length_3656_cov_2.827270"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PKJ17_04040"
     CDS             <1..460
                     /locus_tag="PKJ17_04040"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOD75172.1"
                     /translation="DMITLVPEKRQELTTEGGLDVIKFAGKIEKVIAAVKKRGIAVSL
                     FIDPVEPQIRASAGIGADMIEIHTGSYAEARTARMTKRELDKVIRAAVLGKELGLGVN
                     AGHGLHYHNAREIAVIPQIDELSIGHSIIARALFVGLDRAVKDMIEVIKK"
     gene            577..900
                     /gene="acpS"
                     /locus_tag="PKJ17_04045"
     CDS             577..900
                     /gene="acpS"
                     /locus_tag="PKJ17_04045"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOD75173.1"
                     /translation="MIGIDIVDIARIGGVLERHGERFLEKVFSASEIARAHGTKRLQE
                     YLAGRFAAKEAFMKARGGRLPWRDIEVLSEKGRPFIVYRGRRYDGVSISHERSYAVSV
                     VICGD"
     gene            911..2494
                     /locus_tag="PKJ17_04050"
     CDS             911..2494
                     /locus_tag="PKJ17_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOD75174.1"
                     /translation="MKHMKVLSPKRMARYDEYAIKTWGIPSAVLMENAGRNTYRLMKE
                     RYLAGGETIAVICGRGNNGGDGFVIARYALIGGFRVRAYLIGRKSDLKGDAALNMGLF
                     ESLSGEVIECVEKTKPVRTGIGEADIIVDAIFGTGLSKPVGGMEKTVIDAINASGKPV
                     IAVDIPSGIDGATGKALGSAVHGLHTFTYAYPKPGQVLSPGADHCGSLTVIDISIPSF
                     VEKEVGFDGEITDGDMMRGFLKRRLPSSHKGSFGHAVVIAGSPGKTGAAHMASLAALK
                     IGAGLVTLVIPETLNAILEAKTTEVMTYPVADDGRGFFIPGSFDEIATFVEDKDVVIM
                     GPGLSQNEGVMELVRRIYREIDKPFVIDADGINAFRGHTRLLKKKGGEAVLTPHPGEL
                     ARLIDKTPKEVNDDRMGTALACARAWGVSILLKGAASVLATPDGQMFINPTGNPSLAK
                     GGSGDILTGFIGGLASQGYSLTRSTLLGAYLHGYMADTWVENHSDMDLLALDLVTGLG
                     DAIEEIRNGTDRVYIERSL"
     gene            2457..2885
                     /gene="tsaE"
                     /locus_tag="PKJ17_04055"
     CDS             2457..2885
                     /gene="tsaE"
                     /locus_tag="PKJ17_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOD75175.1"
                     /translation="MGRTEYISKDPSETIDIGEMIGRSASAGEVYAIYGDLGAGKTQF
                     VKGIARGLGVPDWEYVVSPSFTLMNVYEGAMTLCHVDLYRLEEADLGDLNIEEFLAGG
                     IVAVEWAERSRWWEGVIEVRIEISGEMERKIVIIKHENQG"
     gene            2962..>3656
                     /locus_tag="PKJ17_04060"
     CDS             2962..>3656
                     /locus_tag="PKJ17_04060"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOD75176.1"
                     /translation="MLVVQKYGGTSVRDVQRIQNVARRAIEYKGRGMDVIVVVSAMSG
                     ETDRLLNLAHEIAKFPDEREVDVLISTGEQVTSALLAITLREMGHKAVSLLADQIRII
                     SDSSYGTARIASIEDAKLREEIKGGNIIVVPGFQGVDDGGNITTLGRGGSDTTAVALA
                     AALKADLCEIYTDVDGVYTTDPNICEKARRLDKISYDEMLEMASLGAKVLQIRSVELA
                     QKYNVPILVKSSLV"
BASE COUNT          830 a         1053 c         1137 g          636 t
ORIGIN      
        1 cgacatgatc accctcgtcc ccgagaagcg ccaggaactc acgaccgagg gcggcctcga
       61 cgtcatcaag ttcgccggga agatcgagaa ggtgatagcc gcggtcaaga agcggggcat
      121 agccgtttcc ctcttcatag accctgtgga gcctcagatc agggcatcgg ccgggatcgg
      181 cgccgacatg atcgagatcc acacgggttc ctacgccgag gcccgcaccg cgagaatgac
      241 gaagagagag ctcgacaagg tcatccgggc ggcagtgctc ggtaaagaac tcggtctcgg
      301 cgtcaacgcc ggccacgggc tccactacca caacgcgagg gagatcgccg tgatccccca
      361 aatagacgaa ctgtcgatcg gccacagcat aatagcccgc gccctcttcg tcggcctcga
      421 cagagcggta aaagacatga tagaggtgat aaaaaaatga ccaataacca aatcacaatt
      481 gaccaaacgt aacgacagtt cttccccttt ggagaccaag tccggttacc gatcattggt
      541 caattggtta ttggaatttg gtcattggtt attcagatga ttgggatcga tatcgttgat
      601 atagcgcgga ttggcggggt gctcgaaagg cacggggaga gatttctcga gaaggtgttc
      661 tcggcctcgg agatcgcgcg cgcgcatgga acgaaacgct tgcaggaata ccttgccggg
      721 aggtttgccg cgaaggaggc cttcatgaag gcgcggggcg ggcggctccc ctggcgggat
      781 atcgaggtgc tgagcgaaaa aggcaggcct ttcatcgtat accggggcag gcgttatgac
      841 ggggtcagca tctcccacga gagatcgtac gcggtatccg tcgtcatctg cggcgactga
      901 caaagggggc atgaaacaca tgaaggtcct ttcaccgaag aggatggcac gctatgacga
      961 atacgccatc aagacctggg gcatcccctc ggcggtgctc atggagaacg ccggaaggaa
     1021 cacgtaccgg ctcatgaagg aacgctacct cgccggcggc gagaccattg ccgtcatctg
     1081 cggccgcggc aacaacggcg gagacgggtt cgtcatcgcc cgctatgccc tgatcggcgg
     1141 tttccgggtg cgtgcatacc tcatcgggag gaagtcggac ttgaaaggtg acgcggccct
     1201 gaacatgggg cttttcgaat ccctctcggg agaggtcatc gaatgcgtgg aaaagacgaa
     1261 acccgtcagg acaggtatcg gtgaggcgga catcatcgtc gacgccatct tcggaacggg
     1321 cctttcgaaa cccgtgggcg gaatggaaaa gaccgtcatc gacgcgatca acgcctcggg
     1381 aaagcccgtc atcgctgtcg acataccctc cggcatcgac ggggccacgg gcaaggccct
     1441 gggaagcgcc gtccacgggc tccacacctt cacctacgcg tacccgaagc cggggcaggt
     1501 cttaagtccc ggcgccgacc actgcggcag cctcacggtc atcgacatat ccatcccttc
     1561 cttcgtcgag aaggaggtcg gcttcgacgg ggagataacc gacggcgaca tgatgcgggg
     1621 ctttctcaaa aggcgcctcc cctcgtccca caaggggagc ttcggccacg ccgtcgtcat
     1681 tgccggttcg ccggggaaaa caggggcggc gcacatggca tcgctcgccg ccctcaagat
     1741 cggcgccggg cttgtcacac tggtcatccc cgagacattg aacgccatac tggaggcaaa
     1801 gacgacggag gtcatgacat atcccgtcgc ggatgacggc aggggtttct tcatccccgg
     1861 gtccttcgac gagatagcca ccttcgtgga agataaggac gtggtgatca tgggtcccgg
     1921 cctttcccag aacgaggggg tcatggagct cgtacgaagg atataccggg agatcgacaa
     1981 acccttcgtc atagacgccg acggcatcaa cgcgttccgg ggacacacgc ggctcctgaa
     2041 gaagaaagga ggcgaggccg tcctgacgcc gcatccgggg gagttggccc gcctcatcga
     2101 caagaccccg aaagaggtga acgacgatcg gatgggcacg gcgctcgcct gtgcccgcgc
     2161 atggggcgtg agcatcctcc tcaaaggagc cgccagcgtg ctcgccacgc ccgatggcca
     2221 aatgttcatc aaccccacgg gaaatccttc cctcgcgaaa ggaggaagcg gggatatcct
     2281 gacgggcttt atcggcggtc ttgcctccca gggctacagc ctgacccgga gcacccttct
     2341 tggggcgtac ctccacgggt acatggccga cacctgggtg gagaaccatt ccgacatgga
     2401 ccttctcgcc cttgacctcg tgacgggatt gggcgacgct atcgaggaga tccgtaatgg
     2461 gacggacaga gtatatatcg aaagatccct ctgaaactat agacatcggg gaaatgatag
     2521 gccgctcggc ctcggccggc gaggtctacg cgatctacgg cgacctcgga gcgggaaaga
     2581 cccagttcgt caagggcatc gcgcggggcc ttggcgtccc cgactgggaa tacgtggtga
     2641 gcccctcgtt tacgctcatg aacgtgtacg aaggcgcgat gacgctctgt catgtggacc
     2701 tctatcgcct tgaagaagcg gaccttggcg acctcaatat cgaagagttc cttgccggag
     2761 ggatcgtcgc cgtcgagtgg gcggaaaggt cccgctggtg ggagggagtg atagaggtgc
     2821 gcatagaaat tagtggagaa atggaacgga aaattgttat tatcaagcat gaaaatcagg
     2881 gttgagtacc gcggtaaccg cggcgggaac ctctcttgat tcccggtacc atacccttac
     2941 accctatacc tggaggatac aatgctcgtt gtccagaaat atggcggcac gtcggtaagg
     3001 gacgtgcaga ggatacaaaa tgtggccagg cgtgccatcg agtacaaggg gcgcgggatg
     3061 gacgtcatcg tggtcgtctc agccatgtcc ggcgagacgg acaggcttct caacctggcc
     3121 cacgagatag cgaaattccc cgatgaaagg gaagtggatg tgctcatctc cacgggagag
     3181 caggtcacgt ccgcccttct cgcgatcacc ctcagggaga tgggccacaa ggccgtgtca
     3241 ttgctggcgg accagataag gatcatcagc gactcgagct acgggaccgc ccgcatcgcc
     3301 agcatcgagg acgcgaagct ccgggaggag atcaagggag gcaacatcat cgtcgtgccc
     3361 gggttccagg gcgtcgatga cggaggaaac atcaccacac tgggaagggg cggctcggac
     3421 accaccgccg tcgcccttgc ggcggccctc aaggccgacc tgtgcgagat ctacaccgac
     3481 gtggacggcg tctacactac tgatcccaac atctgcgaaa aggcgagacg ccttgacaag
     3541 atatcctacg acgagatgct cgagatggca tccctggggg cgaaggtact ccagatacgg
     3601 tccgtcgaac tcgcacagaa gtacaacgtg cccatcctcg tcaaatcatc gctcgt
//
LOCUS       DAOPOA010000142         3643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_27630_length_3643_cov_3.444259, whole genome shotgun sequence.
ACCESSION   DAOPOA010000142 DAOPOA010000000
VERSION     DAOPOA010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27630_length_3643_cov_3.444259"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PKJ17_04065"
     CDS             <1..1087
                     /locus_tag="PKJ17_04065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD75177.1"
                     /translation="LEFHCAERHKGGSLTMQQDLSIRRYAVGDEPGILKLFAEVFGRN
                     RTAGEWYWQYNDNPWGGGWPVVGVTGNEIVAHYGVARGDLCLRGRRIEAGQSGDAMVR
                     SGMRRNGYCARLMDYCYQLTAADGLKATYGVPNRLSYPGLVRYSGWCKIVNLSYFFFR
                     IGFGKLWGSGPDRVFKCLGGVSSALRMRARRLRHRGDFEISVSSSLPDGLEDLLRNKR
                     TYEVLALWKDPQYLRWRYESHPDVTYLFHTVRTGGIPRALLITRDCGNAIAICDLIHR
                     DNDEVPSLLLLDHIVNYYARSSAQKIEFYGHDAGFFRFVFTSCGFRSIPFSPLVFGGR
                     VFSDPDLDKVFLLPDSWNIAYGDTDVI"
     gene            1127..2161
                     /locus_tag="PKJ17_04070"
     CDS             1127..2161
                     /locus_tag="PKJ17_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HOD75178.1"
                     /translation="MTVHFYNESDRDDWDRYVVDHNSASCYHLTGWKDVIEESFGHRT
                     FYLLSRDEAGAIDGILPLVHLNSLLFGNFAVSLPFFNYGGICADSDGIRDGLFEEAAR
                     FARTRGMAHLELRHSEDLLPGVPVKTSKVSMRLALPGTKEELNASFPSKLRSQVRRAV
                     KEGMVARMGGQEELGSFHSVFSRNMRDLGTPVYSRRFFRAILERFPDAARICTVYTRE
                     GMPVASGMIVGFRDTLEIPWASSLKDFNRYGPNMLLYATVLGFACDAGYRVFDFGRST
                     PDEGTYRFKEQWGAKPAQLYWHYWLRNGRPMPEINPRNPKYGLAIRIWQHLPLGLTRL
                     IGPPLVKNLP"
     gene            2203..3462
                     /locus_tag="PKJ17_04075"
     CDS             2203..3462
                     /locus_tag="PKJ17_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOD75179.1"
                     /translation="MRIRRTLSPTAAPLRPADLWRGLKGFFAGEKYLRRLKEEITGYF
                     GVRHAWFTSSGKAALYVILRALRSLEPDRTEVLIPAYTCFSVPSAIVRAGLEVALCDI
                     DASSLDLNGTLLGKSVNEKTLCVVPDHLFGIPSAMGSIVDLCRDRGVFVLEDAAQAMG
                     GRCDGRFLGTIGDVGLFSLGRGKPVACGSGGIIITASDVIAGAIEREFSSLPFPGRAT
                     QAGEYLKAAAMSLFIHPLLYSLPAALPFLHLGQTVFDSRFPVMRFSSMQAGLCMTWRR
                     RLEAAMSVRKRQAAMLCRMLDAARIAGDVSSAGYLRLPFMTESRERRQRVCRTAKKLG
                     LGITTMYPSPVNEIPQIAHRFAGSTFPAARLVADRLITLPLHRYVRDGDREEMARLLK
                     PVRSIGVRSGVLSVDMTVPGMTGKERV"
BASE COUNT          667 a         1092 c         1147 g          737 t
ORIGIN      
        1 gctggaattt cattgcgctg aaagacataa gggaggatca ctgacgatgc aacaggacct
       61 gtccataaga cggtatgccg ttggtgacga gccgggcatc ctgaagctct tcgccgaggt
      121 cttcggaagg aacaggacgg ctggtgagtg gtactggcaa tacaatgaca atccctgggg
      181 cggcggatgg cccgtcgtgg gtgtgacggg aaacgagatc gtcgcgcatt acggtgttgc
      241 gcggggggac ctgtgcctga gggggcggcg gatcgaggcc ggccagtcgg gggacgcgat
      301 ggtgcgctcc ggcatgcgac ggaacgggta ttgtgcccgc ctgatggact actgctatca
      361 gctcaccgct gccgatggcc tcaaggcgac gtacggtgtt cccaacaggc tgtcatatcc
      421 cgggctcgtc aggtactcgg gatggtgcaa gatcgtgaac ctgagttatt tcttcttccg
      481 gatcgggttc gggaagctct ggggttccgg tccggaccgg gtgttcaaat gtctgggcgg
      541 cgtctcgtcg gcgttgcgga tgcgcgcgcg aaggttgcgc caccgcgggg actttgaaat
      601 atccgtgtcg tcttcgttgc ccgatggcct ggaagatctt ttgcgcaaca agaggacata
      661 cgaggtcctg gccctgtgga aggaccccca atacctcaga tggcggtacg agagccatcc
      721 ggatgtcacg tatctcttcc acacggtgag aacaggcggg ataccccggg cccttctgat
      781 aacgcgggat tgcggcaacg ccatcgccat ctgtgacctt atccacaggg acaacgatga
      841 agtgccgtcc ctcttgctgc tggatcatat cgtcaattat tacgcgcgct catcggcgca
      901 gaagatcgag ttctacgggc acgacgccgg tttctttcgc ttcgtcttca cgtcctgcgg
      961 gttcaggagc atacccttct cgcccctcgt gttcggaggg cgggtgttct cggacccgga
     1021 ccttgacaag gtctttcttc tgcccgacag ctggaacatt gcctatggag acacggacgt
     1081 gatctgagcg cccgccgctt ccttccgaga cgcatcgagg agagacatga cagttcactt
     1141 ttataacgaa tccgaccgtg acgactggga ccgttacgtc gtggaccaca acagcgcgag
     1201 ctgctaccac ctgaccggtt ggaaggacgt catcgaagaa agcttcgggc acaggacctt
     1261 ctatctcctg tcgagggatg aggcgggcgc gatcgatgga atactgcccc tggtccacct
     1321 gaacagcctc cttttcggca acttcgcggt ctccctgccc ttcttcaact acggcggcat
     1381 atgtgccgac agcgacggca tccgtgacgg tcttttcgaa gaggccgcgc gcttcgcccg
     1441 gacgcgggga atggcccacc tggagctgag gcattccgag gacctcctcc ccggggttcc
     1501 cgtcaagaca tcgaaggtgt ccatgaggct cgcgcttccc gggacgaagg aggagctcaa
     1561 tgcctctttc ccttcaaaac tgaggagcca ggtgcgaaga gcggtaaagg aggggatggt
     1621 tgcccgcatg ggcgggcagg aagaactcgg gagtttccac agcgttttct cgcggaacat
     1681 gagggacctg gggacgccgg tctattccag gaggttcttc cgcgccatac tcgaaaggtt
     1741 ccctgacgcg gcccggatat gcacggtgta cacgagggag ggaatgccgg tggcctcggg
     1801 gatgatcgtc gggttcaggg atacgctgga gatcccctgg gcgtcgtcgc ttaaggactt
     1861 caaccgttac ggtcccaaca tgctcctgta tgccaccgtg ctggggtttg cctgcgacgc
     1921 gggctacagg gtctttgact tcggccgttc cactcccgat gaaggcacct acaggttcaa
     1981 agagcagtgg ggcgcaaaac cagcgcagct gtactggcat tactggttga gaaacggccg
     2041 gccgatgccg gagatcaacc cgcgcaatcc gaagtacgga ttggcgatca ggatatggca
     2101 gcatcttccc ctggggctca cgcggctcat aggcccgccc ctcgtgaaga accttcccta
     2161 gcgtaccatg acggactatc cctgccacgg aagacacagc tgatgaggat ccggcgaacc
     2221 ctatccccga ccgcagctcc cctccgcccc gcggacctct ggagggggct caaaggtttc
     2281 ttcgcagggg agaaatacct cagaaggctc aaggaggaga tcacgggata cttcggtgtg
     2341 agacacgcct ggttcacctc gtccggcaag gccgccctgt acgtcatcct ccgcgcgttg
     2401 cgatcgcttg agcccgacag gacggaggtc ctcatacccg cgtacacgtg cttttccgtt
     2461 ccatccgcca tcgtccgcgc ggggctcgag gtcgccctgt gcgacatcga cgcctcgtcg
     2521 ctcgatctca acggcacact tctcgggaaa agcgtcaacg agaagacgct ctgtgtcgtc
     2581 cccgatcatc tcttcggtat cccttcagcc atggggagta tcgtggatct ttgcagggac
     2641 aggggtgtct tcgttctgga ggacgcggcc caggcaatgg gcggcaggtg tgacggcagg
     2701 ttcctgggaa ccatcggcga cgtcggtctt ttcagcctgg gacgcggaaa gcctgtcgcc
     2761 tgcggatcgg gggggatcat catcaccgcc tccgacgtga tagccggagc gatagagagg
     2821 gaattctcgt cgctgccatt ccccggcagg gcgacgcagg ctggagaata cctgaaagcg
     2881 gcggcgatga gcctctttat ccatcctctc ctgtactccc tgcccgcggc ccttcctttc
     2941 ctgcacctcg gccagaccgt cttcgacagc cggttcccgg tcatgaggtt ctcttccatg
     3001 caggccgggt tgtgcatgac ctggcgccgc cgtctggagg ccgccatgtc cgtccggaaa
     3061 aggcaggcgg cgatgctctg ccggatgctc gatgccgccc ggatcgcggg cgacgtgtcg
     3121 tccgccggct acctgaggct tcccttcatg acggaaagcc gggagcgccg gcaaagggta
     3181 tgcaggaccg cgaagaaatt gggcctgggg ataaccacga tgtacccctc gccggtaaac
     3241 gagatccccc agatagcgca ccgtttcgcc ggatcgacct ttcccgcggc ccgccttgtc
     3301 gcggaccgcc tcataacgct gcccctgcac cgttatgtca gggatgggga ccgggaggag
     3361 atggcccgcc ttctcaagcc ggtgcggtct ataggggtcc gaagcggcgt cctcagtgtc
     3421 gatatgaccg tgcccggcat gacggggaag gaaagggtgt gaccatggat gttccggcat
     3481 acgtaaccac ggttttctgg gcggccgttc tcatcgttct ttacgcctac gcgggctatc
     3541 ccgttgtcct cgcggtcctc ggtgctttca gggcgagccg gcccgtctct tcgggcacgg
     3601 atggtcttcc gaaggtgagc ctcctcattt ccgcctacaa cga
//
LOCUS       DAOPOA010000143         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_27826_length_3623_cov_3.464406, whole genome shotgun sequence.
ACCESSION   DAOPOA010000143 DAOPOA010000000
VERSION     DAOPOA010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27826_length_3623_cov_3.464406"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PKJ17_04080"
     CDS             <1..615
                     /locus_tag="PKJ17_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOD75180.1"
                     /translation="GLLFTNSSRAVILIYLSPFVVAAGAHLFLGERLNLLKWMGLVLA
                     FAGLVSVFYGKPSSYNKLMLVGDMLEILAAFLWGATTLYIKKYLAEEMHPINTFLYQL
                     VFSIPVLFLCAWFIEPVWLKDPGTMAISSLVFQSVVIAFASYLVWFKLIHDYPVAKLS
                     VFTFLTPVFGVAFGVLFHGEELTTGLVAGLVMVSAGIYVTNYKK"
     gene            612..1577
                     /locus_tag="PKJ17_04085"
     CDS             612..1577
                     /locus_tag="PKJ17_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOD75181.1"
                     /translation="MNKHRIITAAGFVISIVLLYFSLRGIEYRQLAAAIARADLAYAF
                     LPVCCIFLCLTLCSFRWSMITGNAVRFRDAFIALVIGLFINNVLPARIGEVARGYAIS
                     KRRGIPFTYAVSTVFIDRVFDLLGLLSITFLFFPRQALPPSVSKALYVLVAIFVVCVI
                     VLVAVSRERTARKMARFLEGFKRPFLLRLATRVLEIQENLRRISTPGQIALFIVLSVA
                     NWLSMSSALYFSLKTLGVPLPFVYAPFVTALLNMGLAVPSSPGYIGVYQFLLVYLLAI
                     FGVPKAQSFAVSLFFHASWYIPYNIMGFIFILKEQVHIKDIRKAA"
     gene            complement(1734..2756)
                     /gene="argC"
                     /locus_tag="PKJ17_04090"
     CDS             complement(1734..2756)
                     /gene="argC"
                     /locus_tag="PKJ17_04090"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOD75182.1"
                     /translation="MLNIGIVGATGYTGVVLMSLLLTHPDAKIKLITSNTYRGRKISD
                     IFPLFRGRLEDVLQPTDEDHRGSCDVYFLCLPHGTAMDKAQELFDGKALIVDLSADFR
                     FEDLAIYEGAYVPHTAKELAAQAVFGLPELYREMIKGAKLIGNPGCYPTSAILPLYPL
                     VKHGMLEGDIFIDSKSGVSGAGREGKVGSLFCEVSEGFRAYNVGVHRHEPEIQKEVNK
                     YSPQRISFVPHLVPMSRGILTTVYGRLAKAAKTAEVLDVFRETFKGEPFVRIMDENVY
                     PDTRFVRFSNYCDIGLKVLDDGRIIVISTIDNLVKGASGQALQNMNVALGLPETAGLS
                     GVPQYP"
     gene            complement(2761..3153)
                     /gene="rpsI"
                     /locus_tag="PKJ17_04095"
     CDS             complement(2761..3153)
                     /gene="rpsI"
                     /locus_tag="PKJ17_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOD75183.1"
                     /translation="MPEKRYYATGKRKTAVARVWIKQGSGEFLINGRPFEDYFPVEEL
                     RLTVSKPFVLTNNVGKFDVVANIYGGGIPAQAWALGHGIAKALLEVNSGLRVTLKKQG
                     LITRDPRSKERKKYGKKGARASFQFSKR"
     gene            complement(3146..3592)
                     /gene="rplM"
                     /locus_tag="PKJ17_04100"
     CDS             complement(3146..3592)
                     /gene="rplM"
                     /locus_tag="PKJ17_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOD75184.1"
                     /translation="MKTYFPKEGEVSNDWYVMNADGMVLGRLAARVAMILRGKTKPVF
                     TPHADTGDFVIVVNADKVKLTGAKLLQKTYQKHSNYPGGLRVTNAKTMLEKKPEEVIY
                     QAVKGMLPKNTLGRKILKKLKVYRGSEHPHKAQMPREISLKEVQGA"
BASE COUNT          655 a         1110 c          975 g          883 t
ORIGIN      
        1 ggcctcctct tcaccaattc gtcacgggcc gtcatcctca tctatctgtc gcctttcgtg
       61 gtggccgccg gcgcccatct cttcctgggg gagcggctga acctcctcaa gtggatgggt
      121 ctcgtcctcg cctttgcggg actcgtcagc gtcttctacg ggaagccgtc aagctacaac
      181 aagctgatgc ttgtcggcga catgctggag atcctcgccg ccttcctgtg gggcgccacc
      241 acgctctaca tcaagaaata cctggcagag gagatgcatc ccataaacac gttcctctac
      301 cagctcgtct tttccatccc cgtcctcttc ctttgcgcct ggttcatcga gcctgtctgg
      361 ctcaaggacc ctgggacgat ggcgatatcg tcgctcgtct tccagtccgt ggtcatcgcc
      421 ttcgcgtcat acctggtctg gttcaagctc atccatgatt accctgtcgc gaagctttcc
      481 gtcttcactt tcctcacgcc tgttttcggc gtggcgttcg gagtcctctt tcacggggag
      541 gagctgacaa cgggcctcgt cgccgggctt gtcatggtaa gcgcggggat atacgtaacg
      601 aactataaga aatgaacaag cacaggatca taacggcagc cggtttcgtc atcagcatcg
      661 tccttctcta tttctccctg agggggatag aatacaggca gctcgccgcg gcgatcgcca
      721 gggccgacct tgcgtacgcc ttcctgcccg tatgttgcat cttcctttgc cttaccctgt
      781 gctctttcag atggtccatg ataacgggga acgcggtcag gttccgcgac gcattcatcg
      841 ccctcgtgat cggccttttc atcaacaacg tccttcccgc ccgaatcggg gaggtcgcac
      901 gcggctacgc catctcgaag cgaaggggca tccctttcac gtacgcggtg tcgacggtgt
      961 tcatcgacag ggtctttgac ctgctgggcc ttttgtccat caccttcctc ttcttccccc
     1021 gccaggccct ccccccgtcg gtgtccaagg ccctctacgt cctggttgcc atcttcgtgg
     1081 tatgcgtcat agtgctcgtt gccgtgagcc gggagaggac ggcccgcaag atggcccggt
     1141 tcctcgaagg attcaaacgc cccttcctct tgcgccttgc cacgagggtg ctcgagatac
     1201 aggagaacct ccgccgcatc agcacgccgg gccagatcgc gctcttcatc gtcctgtccg
     1261 tcgccaactg gctctccatg agctcggccc tgtacttctc cctcaagacc ctcggcgtcc
     1321 cccttccctt cgtctacgcc ccctttgtca cggccctcct caacatgggc ctcgccgtgc
     1381 cctcttcccc gggatacatc ggcgtctacc agttccttct cgtctacctc ctcgccatct
     1441 tcggcgtccc caaggcgcaa tccttcgccg tatccctctt cttccacgcc tcttggtaca
     1501 tcccttacaa catcatgggc ttcatcttca ttctcaagga acaggtacat ataaaggaca
     1561 tacgaaaggc ggcttgagtc gcttgaatag cttgagagtg aaaagaagaa agctccgggt
     1621 ggttgggttg cagaaaactg tggtcccggc ttttgccgga gaattagccg tcaaatacct
     1681 tgaggttttt aaccctcaat agattcaagc tattcaagcg actcaagccg cctttagggg
     1741 tattggggta ccccggaaag ccctgctgtt tcgggcaggc cgagggccac gttcatgttc
     1801 tggagggcct ggcccgaggc acccttgacg aggttgtcga tggtggagat gacgatgatg
     1861 cggccgtcgt cgaggacctt gagaccgatg tcgcagtaat tggagaacct gacgaaccgg
     1921 gtgtcggggt aaacgttctc gtccatgatc cggacaaaag gctcgccctt gaaggtctcc
     1981 cgaaagacgt cgaggacctc ggcggtcttt gccgccttgg ccagtctacc gtagaccgtc
     2041 gtcaggatac ctcgcgacat gggaacgagg tgggggacaa aggatatcct ctggggtgag
     2101 tacttgttga cttccttctg tatctccggc tcgtgccggt ggaccccgac gttgtaggcg
     2161 cggaatccct cggagacctc gcagaagaga ctgccgacct ttccctcccg gccggctccg
     2221 ctgacccccg actttgagtc gatgaagata tcgccttcga gcattccgtg cttgacgaga
     2281 ggatacaggg gaagaatggc ggacgtcgga tagcaacccg gattgccgat gagcttcgct
     2341 ccctttatca tctccctgta gagctcgggg agaccgaaga ccgcctgggc ggctagttcc
     2401 ttcgccgtgt ggggcacgta ggcgccttcg tagatcgcca ggtcctcgaa acggaagtcg
     2461 gcgctgaggt ccacgatgag ggcctttccg tcgaacaatt cctgggcctt gtccatggcg
     2521 gtcccgtggg ggagacacag gaaatagacg tcgcaggaac cgcgatggtc ttcgtccgtc
     2581 ggctgaagaa cgtcctccag ccggccccgg aaaaggggaa agatatccga tatcttgcgg
     2641 ccccgatacg tgttggatgt gatgagcttt atcttcgcat cgggatgagt aaggagcaac
     2701 gacatcagga caacgcctgt atagcccgtt gctcctacga ttccgatatt gagcatcgat
     2761 ctatctcttg gagaactgga agctggcgcg ggcgcctttc tttccgtatt tcttccgttc
     2821 cttggatctc gggtccctcg tgatgaggcc ctgtttcttc agggtgaccc tgaggccgga
     2881 gttcacttcg aggagggcct tggcgatgcc gtgacccagt gcccatgcct gggcgggaat
     2941 cccgccgccg tatatgttcg cgacgacatc gaacttgccg acgttgttcg tcagcacgaa
     3001 aggtttgctt accgtgagcc ggagctcttc aacgggaaag taatcctcga acggcctgcc
     3061 gttgataagg aactccccgg aaccctgctt tatccagacc cgcgccacgg cggtctttct
     3121 cttgccagtt gcgtagtacc tcttttcagg caccttgtac ctccttcaac gatatctccc
     3181 tgggcatctg agctttgtgc ggatgttcgc tgccccgata tactttcagc ttcttgagga
     3241 tcttcctgcc gagcgtgttc ttgggaagca tgcccttcac agcctggtag atcacctctt
     3301 caggtttctt ttcaagcatc gtcttcgcgt tcgtcacgcg cagaccgccg gggtagttgc
     3361 tgtgcttctg ataggtcttc tgcaggagct tcgcacccgt gagcttcacc ttgtcggcat
     3421 tgacgacgat gacaaagtca cccgtgtcgg catgaggtgt gaaaaccggc ttcgtcttac
     3481 cgcggaggat catggcaacc ctggcagcga gccttcccag caccatgccg tcggcgttca
     3541 ttacgtacca gtcattcgaa acttcgcctt cttttggaaa atatgtcttc ataaccgttt
     3601 tcacccttaa aaaaatttgt cta
//
LOCUS       DAOPOA010000144         3621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_27848_length_3621_cov_3.442232, whole genome shotgun sequence.
ACCESSION   DAOPOA010000144 DAOPOA010000000
VERSION     DAOPOA010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27848_length_3621_cov_3.442232"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PKJ17_04105"
     CDS             <1..265
                     /locus_tag="PKJ17_04105"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOD75185.1"
                     /translation="PWEERKATFKAYVALYLPEKDRCYVFYGELKGYIGFEEHGSGGF
                     GYDPVFYSPELGKYLAELSTEEKNTLSHRGKAIAALKRFIGNG"
     gene            complement(347..419)
                     /locus_tag="PKJ17_04110"
     tRNA            complement(347..419)
                     /locus_tag="PKJ17_04110"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(383..385),aa:Glu,seq:ctc)
     gene            537..755
                     /gene="tatA"
                     /locus_tag="PKJ17_04115"
     CDS             537..755
                     /gene="tatA"
                     /locus_tag="PKJ17_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOD75186.1"
                     /translation="MFGLGMPELIIILVIVILIFGAGRISEIGGAIGKGIKGFKKSVN
                     EPDSIDVTPDQKDKQEKDKDPQKPIEPK"
     gene            837..1829
                     /locus_tag="PKJ17_04120"
     CDS             837..1829
                     /locus_tag="PKJ17_04120"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOD75187.1"
                     /translation="MEILNGKERASILRAVEALEAGDIVAFPTETVYGLGAVAENPYA
                     VAKIFEVKRRPHFDPLIVHLDGPGRLKGYVSDVPGEARRLMKMFWPGPLTMILRKRPA
                     IPDIVTAGLPTVGVRVPSHPVALELIGALDKPIAAPSANPFGYMSPTRVRHVARSFAD
                     RVPIVLDGGNSTYGIESTIISFREGGVFVHRHGAISVEELSEHVRIVEEVRAADICES
                     PGELPYHYAPMKPLRLVTGPSDARHEASSYLAFRQPVEEVRSRHIKVLSPSGDLREAA
                     SNFFSALIELDRDDVDIIYAEKVPEKGIGRAVMERLRKAARKHPPEAAHNPLNI"
     gene            2097..2330
                     /locus_tag="PKJ17_04125"
     CDS             2097..2330
                     /locus_tag="PKJ17_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75188.1"
                     /translation="MKKVTGKPQPLMENSLISMNESEVRKKLGEPTMVSLTPENKIIW
                     TYRPEWKIMPDNKGTVYVEFDNGKVTKVIKAEQ"
     gene            2327..2590
                     /locus_tag="PKJ17_04130"
     CDS             2327..2590
                     /locus_tag="PKJ17_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75189.1"
                     /translation="MTMLCETCSKEFERTTCPGCKEDVFRFGAYCYLCGGSLAIEAPV
                     EEQTGEDDDFSRRILCSDGTCIGVIGENGVCKVCGKPYAPESE"
     gene            2557..3120
                     /locus_tag="PKJ17_04135"
     CDS             2557..3120
                     /locus_tag="PKJ17_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75190.1"
                     /translation="MRKAVRAGIGIVLFACLFCSCTTYGLYSRNVMVGKNLISEKKYD
                     EAALYLTEAAGYNVDGAAFTYLAVAAYRQGELDRALGYITSAEKSPPDMLTSLRMYGY
                     KALILIGLKDPGAMKALKEYTDRYDSFFPLDSINDVKAMWRTGAVDLPRLEAIIEEQL
                     QTHEEDLELYIYNNVGFYARDNREGAY"
     gene            3132..>3621
                     /locus_tag="PKJ17_04140"
     CDS             3132..>3621
                     /locus_tag="PKJ17_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOD75191.1"
                     /translation="MGFFEKIKNGLTKTRDQLKTKIEWMVAGKPIEEDAFDEIEEALI
                     LADAGLETTELLVDALKDKWKRGLIRTTEDIRTSMKEEVENLLTPIEAPLTANGSKPF
                     VILALGVNGVGKTTTIAKMAGIYLKEGRKVLLGACDTFRAAAIEQLDVWAKRLNVEMV
                     RHA"
BASE COUNT          911 a         1016 c         1012 g          682 t
ORIGIN      
        1 gccctgggaa gagcgcaagg cgaccttcaa ggcctacgtg gccctctacc tgccggaaaa
       61 agaccgctgt tacgtcttct atggggaact caaaggatac atcggcttcg aggaacacgg
      121 ctccggcggt ttcgggtacg accccgtctt ctattccccg gaactcggaa aataccttgc
      181 cgaactctcc accgaagaaa agaacaccct gagccaccgc ggaaaggcga tagcagcact
      241 gaaaagattc atagggaatg gatgagggtt caagggttca aggcgccggt gcctttttgt
      301 ctctcactct tgaactcttg aacttttgaa ccgtcttttg atcatgtggt cccaacgaga
      361 atcgaactcg tgttttcggc gtgagaggcc gacgtcctgg gccgctagac gatgggaccg
      421 ccaagcatga atagtatcag aaatggattc tcatttccac taaaaaaccg acaaaaatct
      481 atacattttg gttgtttcct attataatgg gaagacatca tcataaggag gaaacaatgt
      541 ttggcctcgg aatgccggaa ctgatcatca tacttgtcat cgtgatactc attttcggcg
      601 ccggcaggat ctcggagata ggcggggcga tagggaaagg gatcaagggc ttcaagaaat
      661 ccgtcaacga gcccgattcc atcgatgtca cgccggacca gaaagacaag caggaaaaag
      721 acaaggaccc ccagaaaccg atcgaaccga agtaagacca tcctatcttc ccgtacgtca
      781 agaacaggca acagacccct gccctccggc ttcatgccga ccgaacgcga gaacccatgg
      841 agatcctgaa cggcaaggag agagcgtcga tcttaagggc ggtggaggcc ctcgaggcgg
      901 gcgacatcgt cgccttcccc acggaaacgg tgtacgggct cggggccgtc gccgagaatc
      961 cctatgccgt ggcaaagatc ttcgaggtca agagaaggcc ccatttcgac cccctaatag
     1021 tgcaccttga cggaccgggg cgattgaagg gctacgtgag cgacgtcccc ggagaggcgc
     1081 gacggctcat gaaaatgttc tggcccggcc ccctcaccat gatcctcagg aaaaggcccg
     1141 ccattcccga catcgttacc gccggccttc ccacggtggg tgtacgcgtc ccttcccatc
     1201 ccgtcgcgtt ggaactcatc ggtgccctgg acaagcccat cgccgcgccg agcgccaatc
     1261 ccttcggcta catgagcccc acccgggtac ggcacgttgc caggtccttc gcggaccgcg
     1321 tacctattgt tctcgacggc gggaactcca cctacggcat cgagtcgacc ataatatcct
     1381 tccgcgaggg aggagtgttc gtccaccgcc acggggccat aagcgtcgag gagctgtcgg
     1441 agcacgtgcg aatagtcgag gaggtacggg cagccgacat atgcgagtca cccggcgagc
     1501 ttccctatca ttacgcccct atgaaaccgc tgcggctcgt caccggccct tccgacgcac
     1561 ggcacgaggc atcctcctac cttgccttcc ggcagcccgt ggaagaggtg cggtcgcggc
     1621 acatcaaggt cctctccccg tcgggcgatc tcagggaagc ggcctccaat ttcttttccg
     1681 ccctcatcga actcgatcgt gacgacgtgg acatcatcta tgccgaaaag gtgccggaaa
     1741 agggcatcgg ccgggcggtc atggaacggc tcaggaaggc ggcccgcaag caccctccgg
     1801 aggcggccca caacccgttg aatatttaaa tgaaccgtga taaggtatca ccggaagcgc
     1861 gatcggagca aaacgggaag caatgacgaa agccattgat aagattgaaa aaacattttt
     1921 gcacgatctt tgggcaaagg ggaaatagca tgagtataat caataggtta caagtgtctt
     1981 tatacaattt gtcaacagct tttccacaaa aattgtggaa aagtataaaa agtccccgct
     2041 tttcagggac ctgcatcgta cttctgtcct gttttgttct gctttcctgt tcatacatga
     2101 agaaggttac gggaaaaccg cagcccctca tggagaactc cctcatctca atgaacgaga
     2161 gcgaggtccg caagaaactg ggcgaaccca ccatggtaag tctgacgccg gagaacaaga
     2221 tcatctggac atatcgcccc gagtggaaga tcatgcctga caacaagggc acggtctacg
     2281 tggaattcga caacggcaag gtaacaaaag tgatcaaggc ggagcaatga ccatgctatg
     2341 cgagacgtgt tcgaaggaat ttgaaagaac aacctgcccg ggctgcaagg aagacgtctt
     2401 ccggtttggc gcttactgct atctttgcgg aggctcactg gcgatcgagg cgcccgtgga
     2461 ggaacagacc ggggaagatg acgacttttc gaggaggatc ctgtgcagcg acggaacctg
     2521 catcggtgtg ataggcgaga atggcgtctg caaggtatgc ggaaagccgt acgcgccgga
     2581 atcggaatag tactctttgc ctgcctcttc tgttcctgca cgacctacgg cctctattcc
     2641 cgcaacgtta tggtcgggaa gaaccttatt tccgaaaaga agtacgacga agcagcactg
     2701 tatctcacgg aagccgcagg gtataatgtg gacggcgcag cgttcacata cctcgccgtc
     2761 gcagcctacc gccagggaga actggacagg gccctcggct acatcacaag cgcggagaaa
     2821 tcgccgcccg atatgctaac gtcgcttcgc atgtacggct acaaggcgct catcctgatc
     2881 ggactgaagg acccgggcgc catgaaggcg ctcaaggaat acacagaccg ctatgattcg
     2941 ttcttccccc ttgactccat caacgacgta aaggcgatgt ggcggacggg cgctgtcgat
     3001 ctgccccgcc tcgaggccat catcgaagag caattgcaga cacacgaaga ggacctggaa
     3061 ctctatatat ataataatgt aggtttctat gcccgggata accgggaagg agcatactga
     3121 aggacaacac catgggtttt tttgaaaaga taaagaacgg cctgacgaag acccgggacc
     3181 agctcaagac gaagatcgaa tggatggtcg cgggaaaacc gatcgaagag gatgccttcg
     3241 acgagattga ggaggcgctg atactggccg atgccggact cgagacgacg gagctcctgg
     3301 tggacgccct caaggacaaa tggaagcgcg gactcatccg caccaccgaa gacataagga
     3361 cctcaatgaa ggaagaggtg gagaatctcc tcacccccat cgaggccccg ctcaccgcga
     3421 acggcagtaa gcctttcgtc attcttgccc tgggcgtgaa cggcgtcggc aagaccacga
     3481 ccatcgccaa gatggccgga atctacctga aggagggcag aaaggtcctc ctcggcgcct
     3541 gcgacacctt ccgggcggcc gccatcgagc agctcgacgt ctgggcgaaa cggctcaacg
     3601 tggaaatggt acggcatgcc g
//
LOCUS       DAOPOA010000145         3602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_28037_length_3602_cov_3.373555, whole genome shotgun sequence.
ACCESSION   DAOPOA010000145 DAOPOA010000000
VERSION     DAOPOA010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28037_length_3602_cov_3.373555"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKJ17_04145"
     CDS             <1..295
                     /locus_tag="PKJ17_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOD75192.1"
                     /translation="RMSGDVELLFNEAAVSIGKMIGGQMSLNNQLDCTIERLVTGEIL
                     TQVILSFRGEHLTSLTTTMSARRLDLKAGDRVTAFIKTNEVMLREPDEAGNGN"
     gene            285..1181
                     /locus_tag="PKJ17_04150"
     CDS             285..1181
                     /locus_tag="PKJ17_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD75193.1"
                     /translation="MEIELSVRKKLFTAAGEFPFTADLAIASGEMVSFFGPSGVGKTT
                     MLRIIAGLTDADEGFVRVGGQVWYDSTGGTNLPPQRRKVGFVFQEYALFPNMSVRENL
                     RFAQPKTDKRHIDELLDIFELSTLQHRKPGFLSGGQNQRVALARALARKPDLLLLDEP
                     LSALDHEMRNTLQDEILEVHRLWGITTILVSHDLPEIFKLCNRVIAFRDGRVVDDGNP
                     YDIFGRSKVSGKLQFVAEVLKVEKEDIVDVLTLLIGNSPVKVAVCNNRQETFMPGDKA
                     LVVSKAFNPIIQKLNSNGKGGN"
     gene            1187..1933
                     /gene="modA"
                     /locus_tag="PKJ17_04155"
     CDS             1187..1933
                     /gene="modA"
                     /locus_tag="PKJ17_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOD75194.1"
                     /translation="MKGKPIIVGTALCLLLLFLGVSLASAQTLMIASAAGYKKPVSEA
                     IRSFEKATGIKTDAAFGNIQMVSSQAKQTGEISCIIGDRKFLTALGSTVAFVSYEPIG
                     KGVLVLAYRKGVSLDKVEDMASDKVKTVFMPQDKKAIYGIAGTEALKSYGYTSKLAGK
                     ITQVATVPQVVSYLIAGEADAGFINLTEAMANKERLGGYLVVPEDRYTEILIVAGVVK
                     GFENKPETKKFTDYLKTREAKGIFGKYGVK"
     gene            1935..2633
                     /gene="modB"
                     /locus_tag="PKJ17_04160"
     CDS             1935..2633
                     /gene="modB"
                     /locus_tag="PKJ17_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOD75195.1"
                     /translation="MPHTLATDYLTHPAIALTLKVTLVALILQLAAGLPLGLYLAGKK
                     SPAKTVVEIITTLPMIFPPMALGFFLLLVLGKNGPLGHLLMEVFAFKIIFTPSGVLVA
                     VFVVGLPFMVKSVQAARQQMDGTLIEAAATLGKTRAQTLTKVVLPNIKGGIVTGLLLA
                     FGRSVGEVGISLMLGGNIVGRTETLSLAIYNAVFEGEFLRAAIFSIILTVIAIAVLLV
                     LNTVNRKRIPGLGM"
     gene            complement(2651..>3602)
                     /locus_tag="PKJ17_04165"
     CDS             complement(2651..>3602)
                     /locus_tag="PKJ17_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75196.1"
                     /translation="AGPCRACAGWTACRGRFAAAAAEGGCSSFFREMAQIALTRARQA
                     EARACLAPAAAPAPPSETENREEGEEGPTQPLQGTGGPTWIDLRGGPAQALIVSGVLK
                     QVVELHPGRRYALVARTGLTRILEKHPAIEMSGHPPKGARVLTIRAGSFPGPQEGEYQ
                     RLAASFGLATPVEERLWVPWGFEDERNLMALIPRKEKNILICTSRAPHNVIPIERWEA
                     LAAMLGREGMGVVQTGGRKDPSVRGAYNILGLLTERQFISLPRHFDAVVTSDALMTCA
                     ARLCRTPVVILGDSLGPDSTGLSLAAVRRAVARAVEGQQG"
BASE COUNT          726 a         1059 c         1148 g          669 t
ORIGIN      
        1 gcgcatgtcg ggagatgttg agctcctgtt caatgaagcg gcggtttcga taggaaagat
       61 gatcggggga cagatgagcc tcaacaacca gcttgattgc accatcgaga ggcttgtgac
      121 gggggagata ttgacgcagg tgatactgtc cttcaggggt gaacacctga cctccctgac
      181 cacgacgatg tcggcgagga ggctggacct taaggcgggc gaccgcgtga cggccttcat
      241 caagacgaac gaagtgatgc tgagggagcc ggacgaggcg gggaatggaa attgaactgt
      301 cggtgaggaa gaagctgttc acggccgcag gggagttccc tttcacggcc gacctcgcca
      361 tcgcctccgg tgagatggtg tccttcttcg gcccctcggg cgtgggcaag acgacgatgc
      421 tgcgcatcat cgcgggcctg accgacgccg acgaagggtt cgtgagggtc ggcggtcagg
      481 tgtggtacga ctcgacgggc ggaacgaacc tgccgcccca gaggcgcaag gtcgggttcg
      541 tgttccagga gtatgctctt ttcccgaaca tgagcgtccg cgagaacctg cgcttcgcgc
      601 agccgaagac ggacaagagg catatcgacg agctgctcga catcttcgag ctgtccactc
      661 tgcagcaccg caagccgggc tttctttccg gcggacagaa tcagagggtg gccctggcga
      721 gggccctggc gcggaagccg gacctgctgc tcctcgacga gcccctttcc gcgctggacc
      781 atgagatgag gaacaccctt caggacgaga tcctcgaggt ccacaggctg tgggggataa
      841 cgacgatcct tgtcagccac gacctgccgg agatattcaa attgtgcaac cgggtgatag
      901 cattcaggga cgggcgtgtc gtcgatgacg gcaatcccta cgatatcttc ggtcgaagca
      961 aggtcagcgg caaactgcag ttcgtcgcgg aagtgctcaa ggtcgagaag gaggacatcg
     1021 tggacgtact gaccctcctt atcgggaact cgcccgtgaa ggtcgccgtc tgcaacaacc
     1081 ggcaggagac gttcatgccc ggtgacaagg ccctcgtggt ctccaaggcc ttcaacccca
     1141 tcattcaaaa actgaattcc aacggcaaag gaggcaattg aggcatatga aaggaaaacc
     1201 cattattgtc ggaacggcac tgtgtcttct ccttctcttc ctgggtgtgt ccctggcgtc
     1261 ggcccagacc ctgatgatag cgtcggcagc gggatataag aagcctgttt cggaggcgat
     1321 aagatccttc gaaaaggcca cggggataaa gaccgacgcc gctttcggga acatccagat
     1381 ggtctccagt caggcaaagc agaccggtga gataagctgc atcatcggcg acaggaagtt
     1441 cctgaccgcc ctcggttcca cggtggcatt cgtctcctat gaaccgatag gcaagggtgt
     1501 ccttgtcctc gcctatcgga agggagtgag ccttgacaag gttgaggaca tggcatccga
     1561 caaggtgaag acggtattca tgccgcagga caaaaaggcc atctacggca ttgcggggac
     1621 ggaagcgctg aagtcctatg gttatacatc aaagcttgcc ggcaagataa cacaggtggc
     1681 caccgtgccc caggttgtct cctaccttat cgccggagaa gccgacgcgg ggttcatcaa
     1741 cctcaccgag gccatggcga acaaggaacg gctgggcggc tatctcgtcg tgccggaaga
     1801 caggtacacc gagatactta ttgtcgccgg ggtggtgaag ggttttgaga acaagccgga
     1861 aacgaagaag ttcaccgatt atctcaagac gagggaagcg aagggaatat tcgggaaata
     1921 cggcgtgaaa taacatgccc catacgctgg caacagacta tctgacgcac ccggccatcg
     1981 cgctcacgct caaggtgacc ctggtggcgc tcatcctgca gctcgcggcc ggtctgcccc
     2041 ttgggttgta cctcgcgggg aagaaaagtc cggcgaagac cgtcgtcgag atcataacca
     2101 ccctgccgat gatcttcccg ccgatggcgc tggggttctt cctgctcctt gtcctcggca
     2161 agaacggacc tctcgggcat cttctgatgg aggtgttcgc gttcaagata atattcacac
     2221 cctcgggggt gctcgtcgcc gtcttcgtgg tgggcctgcc tttcatggtg aaaagcgtgc
     2281 aggcggcgcg gcagcagatg gacggcacgc tgatcgaggc ggccgccacc cttgggaaga
     2341 cgagggccca gacgctcacc aaggtggtcc ttcccaacat caagggcggc attgtgacgg
     2401 gcctcctgct cgccttcgga cgctccgtgg gggaggtcgg cataagcctc atgctcggcg
     2461 gcaacatcgt cgggcgcacg gagacactgt cgcttgccat ctacaacgcc gtcttcgaag
     2521 gagagttcct cagggcggcg atattttcca tcatactcac cgtaatagct atcgccgtgc
     2581 tccttgtcct caacacggtg aacagaaaga ggataccggg cctgggcatg taatgccggc
     2641 gggagaaact ctacccttgt tgcccttcga ccgcccgggc cacggcgcgg cggacggccg
     2701 cgagcgagag gcccgtgctg tcggggccaa gcgagtctcc gaggattacg acaggggtcc
     2761 ggcagaggcg ggcggcacac gtcatgaggg catcagaggt gaccaccgcg tcaaaatggc
     2821 gcggcaggga tatgaactgc ctttcggtga gaaggccgag gatgttgtag gcgccgcgga
     2881 cagaggggtc tttccttcct cccgtctgca cgactcccat tccctccctg ccgagcatcg
     2941 ccgccagagc ctcccagcgc tcgatgggga tcacgttgtg cggggcgcgg gaggtgcaga
     3001 tgaggatatt cttctccttc cgggggataa gggccatgag gttccgctca tcctcgaacc
     3061 cccaggggac ccagagacgc tcctcaacgg gtgtcgccag gccgaaagag gcggcgagcc
     3121 tttgatattc cccttcctgt ggacccggaa agctcccggc ccggatcgta aggacccggg
     3181 cgcccttcgg cggatgcccg ctcatctcta tcgccgggtg tttctcgagg atccgtgtga
     3241 gcccggtccg tgcgacgaga gcataccgcc ttccggggtg cagttccacc acctgcttga
     3301 gcacccccga cacgataagg gcctgggcgg gaccgccgcg gaggtctatc caggtgggac
     3361 ctccggttcc ctgcaaaggt tgggtgggcc cttcctctcc ttcctcgcgg ttttccgtct
     3421 ccgaaggtgg ggccggtgct gcggcaggcg cgaggcaggc gcgggcctcg gcctgcctcg
     3481 cgcgggtcag cgcgatctgt gccatctccc ggaagaatga ggaacacccc ccttccgcgg
     3541 ctgcggcggc aaatcttccg cggcatgccg tccatcccgc gcaggccctg cacggaccgg
     3601 ca
//
LOCUS       DAOPOA010000146         3592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_28147_length_3592_cov_3.860616, whole genome shotgun sequence.
ACCESSION   DAOPOA010000146 DAOPOA010000000
VERSION     DAOPOA010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28147_length_3592_cov_3.860616"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PKJ17_04170"
     CDS             <1..401
                     /locus_tag="PKJ17_04170"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75197.1"
                     /translation="FKPAKDQYRVFHVRSDQTMDDVAMITEVLTRRVKNTDLGPLPDL
                     FIIDGGKGQLAAAHRVLKANNVDRDIISIAKGERRRKMEDVIYVPARKNPLALPRASA
                     VFREIVKMRDEAHRFAVASHRRWRRKENLK"
     gene            complement(545..2476)
                     /locus_tag="PKJ17_04175"
     CDS             complement(545..2476)
                     /locus_tag="PKJ17_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75198.1"
                     /translation="MKLKDIHTESMKLQWNFYERTINNYSIIMRIMQSAEKDLLISHV
                     PRVFVEESFFDTCKIMVDEDGIVGEGFYSIDENLNSLDYATVAAFNGSSATASLVDDV
                     FGYGLLYIYPLRRELRTIGYLVLGKRYWIDVELRLLRELEIVCDIYNRSLLLRSGVAR
                     RKTGVHSAATFEKVLEVFPDPLVLIDRKGYICYANRNAKAQFETKKGFFLGERADRAI
                     PGLPEDLVKKNCPFRGEIRYKLQDDYRMFRVESFAVRNADENGEWRAIIFKDVVKMKV
                     NEEEHLLRKRTESVGMLAGGIAHDFNNMLTGILGYAALMRKLLSDAKLSRYAEAIEHS
                     AQRASKLTKHLLNFSRRQKRSTGLVDVNALLDDVLFLLKQSFFDIRIERDFDTELPNI
                     KGDEAELQNVFLNLFINARDAMEGDGLLRIATSRQDKGYVCIEIQDTGKGIDEALQHK
                     IFEPYFSTKESVSNLGMGLYLVDKVVKDHGGFIEIVSEKEKGTIFYLYLPAPSLRVSE
                     AKPREAPLHGQNLSDRTILVVDDEYLVRELLKGILSETGAKILEADNGEDALEIYSRH
                     QDEIDLVVLDVIMPGMKGDEVLRRIRKTREDMKVVISSGFMSEQQRAKLKEYRVDGFL
                     DKPFKDDDALSLIAEVLSE"
     gene            complement(2481..3278)
                     /locus_tag="PKJ17_04180"
     CDS             complement(2481..3278)
                     /locus_tag="PKJ17_04180"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOD75199.1"
                     /translation="MPREQLINRASEIKVIPTLDSIVNRVFTVLGNNNSSFNDLSEVV
                     KYDQAISSKIISIANSAYYSRGIEIFNLQRAMLTIGFEEVRSIVSCILLMEGIIKMLK
                     LKEEDLLDLWKHSIEVASAARVLAERLRIEDPQKAYTASLLHDIGKIVFYLAVPDYGE
                     MLKETKSAGDIVALERERFGIDHQETGYIIAVKWKFPHDFARVIRGHHGNQSPDNPDS
                     LLRVVNASDRFFTRTLSGQSTEGFILDKERDAITLEVEKIMEFLQLG"
BASE COUNT          764 a         1009 c          975 g          844 t
ORIGIN      
        1 atttcaagcc agcgaaggac cagtaccgcg ttttccacgt ccggtcagac cagaccatgg
       61 atgacgtggc catgataacg gaggtgctca cgcgccgggt gaagaacacc gatctcggcc
      121 ccctgccgga ccttttcata atagacgggg gcaagggaca gctcgcggcg gcccaccggg
      181 ttctcaaggc caacaacgtc gacagggaca tcatctccat agcaaaagga gagcgccgca
      241 gaaaaatgga agacgtcatc tacgtcccgg ccagaaagaa ccccctcgcc ctgccccggg
      301 catcagccgt cttcagggaa atagtgaaga tgcgcgacga agcacaccgc ttcgccgtgg
      361 cctcgcacag aaggtggaga aggaaggaga atttgaaata gggaaaagac gcacacgggt
      421 ctgcacttcg tgcaaacggg tcggcgctca cgcgcctaac gagtgaacgg gcaaaagaag
      481 aagaaaaaga ggtacgatct tccccgtgtg cccgtgtgcc cgaatggcag acccgtatgc
      541 ccgtctactc ggacagcact tccgctatca gcgagagtgc gtcgtcgtcc ttgaagggtt
      601 tgtcgaggaa accgtcgacc ctgtattcct tcagctttgc ccgttgctgt tcgctcatga
      661 atccacttga tatcacaacc ttcatgtcct cgcgggtctt ccggatacgc cggagcacct
      721 cgtccccttt cattcccggc atgatcacgt cgaggaccac aagatcgatc tcgtcttgat
      781 gccgcgaata tatctcgagt gcatcttcgc cgttatcggc ctcgaggatc ttggcgcccg
      841 tttcggaaag gatgcccttg agcagttcac ggaccaggta ttcatcgtcc acgacaagga
      901 tggtcctgtc gctcaagttc tggccgtgaa gcggcgcctc cctcggcttt gcctcggaca
      961 cccgcaggga gggggccggc aggtaaaggt agaagatggt tcccttttcc ttctcgctga
     1021 cgatttcgat gaagccgccg tggtccttta cgaccttgtc gacgaggtac aggcccatcc
     1081 ccaggttcga gacactctcc ttggtggaga aatagggctc gaatatcttg tgctgcagcg
     1141 cctcgtcgat ccctttgccg gtgtcctgta tctcgataca gacatagccc ttgtcctgcc
     1201 gcgaagtcgc aatgcgcaag agaccatctc cctccatcgc gtctctcgcg ttgatgaaga
     1261 ggttcaggaa gacgttctgc aattcggcct cgtccccctt tatgttgggt agctcggtgt
     1321 caaagtccct ttcgatgcgg atgtcgaaga aactctgctt cagcaggaag agcacgtcat
     1381 cgagaagggc gttgacatcg acaaggcccg tcgacctctt ctgccggcgg gagaaattga
     1441 gaagatgttt cgtcagcttg gatgcgcgct gggctgagtg ctcgatcgcc tccgcgtagc
     1501 gggacagctt tgcgtcggaa agaagctttc tcatcagtgc cgcgtatccg agaatgcccg
     1561 tgagcatgtt gttgaagtcg tgggcgatgc ctcccgccag catccccacg ctctcagtcc
     1621 gtttcctcag gaggtgctct tcttcgttga ccttcatctt caccacatcc ttgaagataa
     1681 tggcccgcca ttccccattc tcgtccgcgt tcctgacggc aaagctctcg acacggaaca
     1741 tcctgtaatc atcctgcagc ttgtatcgta tctccccgcg gaaggggcag ttcttcttga
     1801 cgaggtcctc cggcaggccc gggatggccc tgtccgcacg ttcgccgagg aaaaaacctt
     1861 ttttcgtctc gaattgcgct ttcgcgttcc tgttcgcgta gcagatgtag cccttcctgt
     1921 cgataaggac cagcgggtcg gggaagacct cgagcacctt ctcgaaggtg gccgccgaat
     1981 ggacccctgt tttgcggcgg gcaaccccgc tgcggaggag cagagatctg ttgtatatgt
     2041 cgcagacgat ctccagctcc ctgagaagcc tgagttcgac gtctatccag tacctcttgc
     2101 cgaggacaag atatccgatc gtcctgagtt ccctgcggag cggataaatg tagagaaggc
     2161 cgtacccgaa gacgtcatcg accagcgacg ccgtggcgga gctgccgttg aaagcggcca
     2221 cggtcgcgta atcgagactg ttcaggttct cgtcgatgct gtagaacccc tcgcccacga
     2281 tgccgtcctc gtcgaccatg atcttgcatg tgtcgaagaa ggactcttcc acgaagaccc
     2341 tgggaacatg gcttatgagc aggtccttct ccgcgctttg cattatgcgc attatgatgg
     2401 aataattgtt tatggtcctc tcgtagaagt tccactggag cttcatcgac tcggtatgta
     2461 tgtccttcag tttcatctcg tcaacccaat tggagaaatt ccattatctt ctcaacttcc
     2521 agcgttatgg cgtccctttc cttatccagt atgaaacctt ccgtcgattg accgcttaat
     2581 gtccgcgtaa agaaacggtc cgacgcgttc accacccgga gcagggaatc cggattgtcc
     2641 gggctctgat tgccatggtg gccgcgaata acacgggcaa agtcgtgggg gaatttccac
     2701 tttacggcta tgatgtatcc cgtttcctgg tggtcgatgc caaaacgctc gcgttcgagg
     2761 gcaacgatat ccccggcgct cttcgtctct ttcagcattt ccccgtagtc gggaacggcg
     2821 agataaaaga cgatcttgcc gatatcatgc aacagtgacg cggtgtaggc cttctggggg
     2881 tcctcgatcc gaagccgttc ggcaagcacg cgcgccgccg aggccacctc gatggaatgc
     2941 ttccacaggt ccagaaggtc ttcctccttg agcttgagca tctttatgat gccttccata
     3001 agcaggatgc agctgacaat gctgcgaacc tcctcaaaac ctatggtcag catggcccgc
     3061 tgcaggttga atatctcgat gcccctgctg taatatgccg aattggcgat gctgatgatc
     3121 tttgaagaaa tggcctggtc gtacttgacc acctcggaaa gatcgttgaa ggaagagttg
     3181 ttgttgccga gcacggtgaa caccctgttc acgatgctgt ccagcgtcgg gatcaccttg
     3241 atctcgctgg cccgattgat gagttgctct cttggcattg catgagtcat agtcgtataa
     3301 tcgggttttc ggtgaaaaag acaatacatt aagctttttc ttatattttt cccgggaggg
     3361 tcaggagccc tccgaaaagc gcgtctgacg gaattcatct tgactttcgg ggagtttttg
     3421 actatgatgt tacaattttt tggaggtgtc caccatggga ttaggtctta taggcaagaa
     3481 actaggcatg tcgcagatat tcgacgaaca cgggaacgtt gtccccgtta cggtcataaa
     3541 agccggtccg tgctacgtgg tccagaagaa gaccgccgat aaggaaggtt ac
//
LOCUS       DAOPOA010000147         3589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_28171_length_3589_cov_3.440294, whole genome shotgun sequence.
ACCESSION   DAOPOA010000147 DAOPOA010000000
VERSION     DAOPOA010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28171_length_3589_cov_3.440294"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1193)
                     /locus_tag="PKJ17_04185"
     CDS             complement(12..1193)
                     /locus_tag="PKJ17_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75200.1"
                     /translation="MAKDVAVIGVGQTYFVRGYEGSIRELAFEAFREAMQDANLTNKD
                     VDASIFCSAPEYDKQRSPAGVLAEYLGLVPQPTFYVETVCSSSTVGVKIGYSMIKAGL
                     HDIVVVLGFQKMSEITSAESQERMGRGGDIQWEAPFGTMMPAYYALYAQAYLAKNGLT
                     LDDLRDVRVKAATYGQINERAVYRKGVKPEDFDPNNPDAKMAGPVASPLRVGDCCANA
                     DGASCIILANAEKAKVFSKKPVWILGIGAASEVVNMAARPDFSKGIQVAKAAAGQAYK
                     MAGLGPKDIDVAEVHDCFTIAEIMAYEGLGFAPEGDGKQLIREKATYKEGRIPVNVDG
                     GLLSKGHPIGATGGSQIRTVVLQLRDEAGPMQVKDPQIGLNHNIGGVGLYGNVTIFGR
                     D"
     gene            complement(1208..2206)
                     /gene="mltG"
                     /locus_tag="PKJ17_04190"
     CDS             complement(1208..2206)
                     /gene="mltG"
                     /locus_tag="PKJ17_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOD75201.1"
                     /translation="MLAARDKKTIVIAIIIFIFSQGLIFACIPRDDDRSIRPVVVKQG
                     TGVAEIANALKKEGIIRSSALFMIGSLMYRGHLIAGEYELTRDMSTFEIIRKMGQGKR
                     KIYVLKIIEGNNIYTIAESVGRSGIMTREEFLELSTDKKYLESMGIEADSMEGYLSSN
                     TYYYSKEIDREKFLKKIALRTLKLFSNEDIRRKMASLGMNTHQTLTLASMIEKESGAK
                     DEKPLVSAVFHNRILKGMSLDCDPTVIYGTKGFGSPIRKIDLATRTPYNTYMLKGYPR
                     GPICTPSKDSIMAALNPAPVDYLYFVSKNDGTHVFSRDMAEHNRYVEMYQRNRTTQ"
     gene            complement(2187..2603)
                     /gene="ruvX"
                     /locus_tag="PKJ17_04195"
     CDS             complement(2187..2603)
                     /gene="ruvX"
                     /locus_tag="PKJ17_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOD75202.1"
                     /translation="MRILGLDVGDKKIGVSLSDPTLSIAQGLKLYRRASLEEDVEELK
                     RIVEEHEVGEIVVGLPRNLNGTIGTRAEKVMGLADRIRESVTIPVTLWDERFSTNEAH
                     RVFDMAEVRHKKRKPYLDIMASQIILQGYLDARSAR"
     gene            2852..3298
                     /locus_tag="PKJ17_04200"
     CDS             2852..3298
                     /locus_tag="PKJ17_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD75203.1"
                     /translation="MEFTTGPVKLKVLTLKDLDAVTDIDESLLGTRRRDYWEVRLERA
                     EVSGVPSLAAELDGRVIGFILGSASGWEYGIPENVAWIDTLGVRKEYQKQGIARLLFQ
                     EMFSMFKKVGVDTIYVFVNWRDWDLLRFFDRMGFKRGDMINLEMKI"
BASE COUNT          713 a         1008 c         1013 g          855 t
ORIGIN      
        1 cgaatcccat cttagtccct cccaaaaatt gtgacgtttc cgtagaggcc cacaccgccg
       61 atattgtgat tgaggccgat ctgaggatct ttgacctgca tggggccggc ttcgtcccgg
      121 agctgaagga caacggtccg gatctgtgac ccaccggtgg ctccgatcgg atggcctttt
      181 gagaggaggc cgccatccac gttcacgggg atcctgcctt ctttgtaggt tgctttctcg
      241 cggatgagct gcttgccgtc gccctcgggc gcgaatccga ggccttcgta ggccatgatc
      301 tccgcgatgg tgaaacagtc atggacttcg gcgacatcga tatctttggg tcccagcccc
      361 gccatcttgt atgcctggcc tgccgccgcc ttcgcaacct ggatgccctt ggaaaaatca
      421 ggtctggcag ccatattgac aacctctgac gcggccccta tgccaaggat ccataccggc
      481 ttcttcgaga ataccttggc cttctcggcg tttgcgagaa tgatgcatga cgcgccatcc
      541 gcatttgcgc agcagtcgcc tacgcgaagc ggcgaggcga cgggtcccgc catcttcgca
      601 tcggggttgt tgggatcgaa atcctcgggt ttcacgccct tgcggtagac ggccctttcg
      661 ttgatctggc cgtaggtggc cgcctttacc ctgacatccc tcaagtcgtc gagggtgagg
      721 ccgttcttgg caagatacgc ctgggcgtag agcgcgtagt aggcgggcat catcgtgcca
      781 aagggggctt cccactggat gtcgccgccg cggcccattc tctcctggga ctcggcggag
      841 gtgatctcgg acatcttctg gaagccgaga acgacgacga tgtcatggag accggccttg
      901 atcatgctgt agccgatctt gacgccgacc gtgctggatg aacacaccgt ctcgacgtag
      961 aatgtcggct gggggacaag gccgaggtac tcggcgagca cgcccgccgg cgatctctgc
     1021 ttgtcatact ccggggcaga gcagaagatg gaggcgtcca cgtccttgtt ggtgaggttc
     1081 gcgtcctgca tcgcttctct gaaggcttcg aaggccagtt ccctgatgga gccctcatag
     1141 cccctgacga agtacgtctg gccaacgccg ataactgcaa catcttttgc catacattcc
     1201 tcctttatta ttgtgttgtc ctgtttctct ggtacatttc gacgtatctg ttatgctctg
     1261 ccatgtcccg ggaaaagaca tgggtaccat cattctttga gacaaagtaa aggtaatcga
     1321 cgggtgccgg gttgagcgcg gccataatgg agtctttcga cggggtgcat atcggccccc
     1381 gggggtagcc tttgagcatg tatgtgttgt aaggagtgcg cgtcgcgagg tcgatcttcc
     1441 gtatggggga tccgaagccc ttcgttccgt aaatgaccgt ggggtcgcag tcgagcgaca
     1501 tccccttgag tatcctgttg tggaagacgg cggagacgag aggtttctcg tccttggctc
     1561 ccgattcctt ctcgatcatc gaagcgaggg tgagtgtctg gtgcgtgttc atcccgaggg
     1621 aagccatctt ccgcctgata tcttcgttgc taaagagctt gagggtcctc agcgcgatct
     1681 ttttgaggaa cttctccctg tcgatctcct tactgtaata atatgtgttt gaagaaagat
     1741 atccctccat agaatcggct tcgatgccca tgctttccag gtacttcttg tccgtggaga
     1801 gttcgaggaa ttcttctctc gtcatgatgc cgcttctgcc gacggattcc gctatcgtgt
     1861 atatgttgtt gccctcgatg atcttcagga cgtagatctt ccgcttgccc tgacccatct
     1921 tccgtatgat ctcgaacgtg gacatatccc tcgtgagttc gtactcgccg gcgatgaggt
     1981 gacccctgta cataagagat ccgatcatga agagggccga tgaacgtatg atgccttcct
     2041 tcttgagggc gttcgctatc tccgccacgc cggttccctg tttcacgacg acaggccgta
     2101 tcgaccggtc gtcgtccctg gggatgcagg cgaatatcaa gccctgcgaa aagatgaaga
     2161 taatgatggc aatgacgatg gtcttcttat cgcgcgctgc gagcatcaag gtatccctgc
     2221 agaattatct gtgatgccat gatatccaga tacggttttc tcttcttgtg gcgcacctcg
     2281 gccatatcaa agaccctgtg cgcctcattc gtgctgaaac gctcatccca gagggtaacg
     2341 ggtatggtca ccgactcgcg gatcctgtcg gcaagcccca tgaccttctc cgcccgtgtt
     2401 ccgattgtgc cgttgaggtt cctgggaagg cccacaacga tctccccgac ctcgtgttcc
     2461 tccacgatac gcttcaactc ttccacatcc tcttcaaggg aagcgcggcg atagagtttc
     2521 aggccctggg cgatcgacaa ggtgggatcg gacaaagaca ccccgatctt cttgtccccc
     2581 acatccagcc cgagtatacg catcaactat tatagcagtt tttgctttca tttcaagaac
     2641 aaaactaccc ggcaggatgt cgccggacga cgcgctctgc aggcaaagac cctcgcaggg
     2701 aacaggaaag atcagaggca aaggcgggga taggaccaat gagacctatt ggtcctgctc
     2761 ttccctctct ccccgtcctg tgttgacaag tcccctttct ctgctatact ccacagtaag
     2821 aaagtaagtt gcgcggggga ggcatagagc catggaattc accaccggac ccgtgaagct
     2881 gaaagtgctc accttgaagg acctcgatgc cgtcaccgac atcgacgaat cactcctcgg
     2941 gacaagaaga agggattact gggaggtaag gcttgagagg gccgaagtgt cgggggtgcc
     3001 atcgcttgcg gccgagcttg acggtcgggt catcggtttc attctcggca gcgcgagcgg
     3061 ctgggaatat ggtatacccg agaatgtcgc gtggatagac actctcgggg tcaggaagga
     3121 gtaccagaag caggggatcg cgaggcttct cttccaggag atgttctcca tgttcaagaa
     3181 ggtcggcgtc gacacgatct atgtcttcgt gaactggaga gactgggacc ttctcaggtt
     3241 cttcgaccgg atgggtttta agaggggaga catgatcaac ctggagatga agatctgaaa
     3301 gagtctttaa cgggccgact ctcgccggcc cagcggtata gaccgcagaa gaccggggcc
     3361 aaaagggatc caccagcccc ggccttcttg tccttaagcc catatcggac caatcccttc
     3421 gttgtcttcg tcgtcggcat cctcgacgta cggacgtacg cctccggcgc cttctcctcg
     3481 ccgcctcggt attgccccga tctgggctta aggacaagag aggtggcttg aatggcttga
     3541 cagcctgttg ataaactctg tctcacggca tgatcccgct cggtggaga
//
LOCUS       DAOPOA010000148         3569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_28383_length_3569_cov_3.960159, whole genome shotgun sequence.
ACCESSION   DAOPOA010000148 DAOPOA010000000
VERSION     DAOPOA010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28383_length_3569_cov_3.960159"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PKJ17_04205"
     CDS             complement(<1..1292)
                     /locus_tag="PKJ17_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOD75204.1"
                     /translation="MRPYFEKMQEWNKPVKVNAKNAEEIKAVEKDIEERVLKARNAGL
                     AQETLNKRGEWTVHQRLEYIVDPGTWAPLHLLFDPMDEESGTTGVVDGLGRINGKWCV
                     IIGFDNKVMAGAWIAGQPLNILRVTDTARRLHCPLVWLVNCSGVKLPEQETMYGNRHG
                     SGTTFFRHAELNKLGIPVLAAIYGTNPAGGGYQGISPTLLLAHKDANIAVGGAGIVSG
                     MAPKGFFDEEMADAIIDATKKFKSIPPGRVEIHHDVTGFFREVHPSEESLLDSLKSWV
                     GKLPYYDPSFFRVAAPAEPKYPTEDLYSVVQFNQKMVYDMEGVMARLVDNSEHMEFRP
                     EIGPELYTGLVKVDGFLIGIVGNRQGMLPKGYPSYAPYPGIGGKFYREGLIKVNEFVT
                     LCGRDRVPMIWIQDTSGIDVGDIAEKAELLGLGQSLIYS"
     gene            complement(1591..2877)
                     /locus_tag="PKJ17_04210"
     CDS             complement(1591..2877)
                     /locus_tag="PKJ17_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75205.1"
                     /translation="MEDIKGMYKKIVKDNFPETIRIDLGGQVLVYRKKVWNIFDADEK
                     CDVERGLRYGENPDQPSAMYELTNGNITLGDVRFFDVETGLVSRVTEKDMLQVGKHPG
                     KINLTDVDNALNILKFLDQKPACAIMKHNNPSGAAYGATTAEAFEKAFWCDRIAAFGG
                     AVVFTKTLDVETAKAMVPYYFEVVCAPDFEGGAIDVLKKWKNLRLMQIKEIERLSAFK
                     TRRTIDFKSLMDGGLIIQESQPFRIRGKEDLKAAEAVYKGETYRIKRMPTDDEYRDML
                     FGWYVESGVSSNSALFVKDEATVAIGTGEQDRVGVVEIAVFKAYKKFADTEVFREFGV
                     PYAVFKLEAERGQRDMKDLDAIDAYTREMNAGLRGSVIVSDGFFPFRDGVDAAIKEGA
                     RGVIQPGGSERDYEVIEACNEQGVTMVFAGQRLFKH"
     gene            complement(3057..>3569)
                     /locus_tag="PKJ17_04215"
     CDS             complement(3057..>3569)
                     /locus_tag="PKJ17_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOD75206.1"
                     /translation="IRYVAAVRGNFFIGMGRNRWPVMYRNEGEPYFAGDYAFEYGKMD
                     LMADGTDGAIITYGGLVSKALGIREKLAGKGKSFAVVNMPCVDEVDETVMAKLATLPS
                     VVTYEDHNVRTGIAPVITASLLKLGFTGTFLSFGATSYGASGETDEVLASQGLDVETV
                     TAATLKALKG"
BASE COUNT          658 a         1076 c          974 g          861 t
ORIGIN      
        1 gagtagatga gtgactggcc gaggccgaga agttcggctt tctcggcgat gtcgccaacg
       61 tcgatacccg acgtatcctg gatccagatc atcgggaccc tgtccctgcc gcagagggta
      121 acgaattcgt taaccttgat gaggccttcg cggtagaact taccgccgat gccgggatat
      181 ggtgcatagc tcggatagcc cttcggaagc ataccctgcc tattgccaac gataccgatc
      241 aggaagccgt caaccttgac aagaccggtg taaagctcgg gcccgatctc cggacggaat
      301 tccatgtgct cgctgttgtc gacgaggcgc gccatgacgc cttccatatc gtaaaccatt
      361 ttctggttga actgaacgac gctgtagagg tcttccgtcg ggtatttcgg ctctgccggc
      421 gctgcaaccc tgaagaagct cggatcgtaa tagggaagct tgcctaccca ggatttcagt
      481 gaatcgagga ggctttcctc tgacggatgg acttcgcgga agaaaccggt cacgtcgtgg
      541 tggatctcaa cgcgtcccgg aggaattgac ttgaatttct tggtggcgtc gatgatggca
      601 tcggccatct cttcgtcgaa aaagcccttc ggtgccatgc cgctgacgat gccggcgccg
      661 ccgactgcga tgttcgcgtc cttatgggcg agaaggaggg tcggggagat gccctggtag
      721 ccgccgcccg cggggtttgt cccgtaaatg gccgcgagga ccgggatacc gagtttgttc
      781 agttcggcgt gacggaagaa tgtcgtaccg ctgccgtgcc tgtttccgta catcgtttcc
      841 tgctcgggaa gcttgacgcc ggagcagttg acgagccata cgagcgggca atggagcctt
      901 ctcgcggtat cggtcaccct gaggatgttc agcggctgac ccgcgatcca tgcgcctgcc
      961 atgaccttgt tgtcaaaacc gatgatcacg caccatttgc cgtttattct tccgagaccg
     1021 tcgacaacac ccgtagtccc cgattcttca tccatcgggt cgaacagcag gtgaagaggt
     1081 gcccaggtgc ccgggtcaac gatatactcc aatctctggt gaacggtcca ttcaccgcgc
     1141 ttgttcaggg tctcctgggc aaggccggcg ttcctggcct tcaggacgcg ttcttcgatg
     1201 tctttctcta cggctttgat ctcttcggca ttctttgcat tgactttaac cggcttgttc
     1261 cactcctgca ttttttcaaa atacggtctc atttattacc tccgatggct gtaggaattt
     1321 gttactgttt tcacaatttt aatttttttt ccagcacttt gtcaacacaa aaggcgggga
     1381 aaggagggtc agggggcagg gaatagggag cagggacgat cacctgcatc ccgactcccg
     1441 acctctaacc gtcagcccct gcatccttac cttttgtcta aaaacggtcc ttcatcttca
     1501 ccctgatgat gacatacacc acgatgatca cgccgattat caggagatac ttcatgcgat
     1561 gcccctcttt tcccgctccc tcgcaccggt ctagtgcttg aagagccgct ggcccgcgaa
     1621 gaccatcgtg acgccctgtt cgttgcaggc ctcgatgacc tcgtagtcgc gttccgaacc
     1681 acccggctgt atgacgcccc gggccccttc ctttattgcc gcgtccactc cgtccctgaa
     1741 ggggaagaaa ccgtcggaga cgatcacgga accccgtagg ccggcgttca tctcccgcgt
     1801 gtacgcgtct atcgcgtcaa ggtccttcat gtcccgctgg ccccgctcgg cctcgagctt
     1861 gaagacggcg tagggcaccc cgaactccct gaagacctcg gtgtcggcaa acttcttgta
     1921 ggccttgaaa acggctatct ccacaacgcc cacccggtcc tgctcgcccg tgccgatggc
     1981 caccgtcgcc tcgtccttca cgaaaagggc ggagttggag ctgacccccg actccacgta
     2041 ccagccgaag agcatgtcgc ggtattcgtc gtccgtgggc atccgtttta tcctgtacgt
     2101 ctcgcccttg tagaccgcct ccgctgcttt gaggtcctct tttccccgta tcctgaaggg
     2161 ctgcgattcc tggatgatca gcccgccgtc catgagggac ttgaagtcta tcgtccttct
     2221 cgtcttgaag gcgctcaggc gctctatttc cttgatctgc atcagccgca ggttcttcca
     2281 tttcttcagg acgtcgatgg cgccgccctc gaagtccggg gcgcaaacga cctcgaagta
     2341 atagggcacc atcgccttcg ccgtttccac atcgagggtc ttcgtgaaga cgaccgcccc
     2401 gccgaaggcc gctatcctgt cgcaccagaa ggccttttcg aaggcctccg ccgtcgtcgc
     2461 gccgtacgcc gccccgctgg ggttgttgtg tttcatgatg gcgcaggcag gcttctgatc
     2521 gagaaacttg aggatgttca gggcattgtc cacatcggtc aggttgatct tccccgggtg
     2581 ttttcccacc tggagcatgt ccttttccgt gaccctgctc acgaggcccg tctcgacgtc
     2641 gaagaaccgc acgtcaccga gggtgatgtt gccgttcgtg agctcgtaca tcgccgacgg
     2701 ctggtcgggg ttctcgccgt accggaggcc ccgctcgacg tcgcatttct cgtcggcgtc
     2761 gaatatgttc cagaccttct tcctgtaaac gagcacctgt ccgccgaggt cgatcctgat
     2821 ggtctcggga aaattatcct tgacgatctt cttgtacatc cccttgatgt cttccacgaa
     2881 gcaccccttt tttagttaat gaccaatgac caatgcacca aaccccaatg accggaagat
     2941 gatcgggaac cgaacaaaga gccaatagca tgattcaggc tttttattcg tttggttaat
     3001 tggttattga tcattgtctc tttactggtt attggaattt tgtcattggt cattgttcat
     3061 cctttcaagg ccttcaacgt tgcggctgtt accgtttcca cgtccagtcc ctggctggcc
     3121 aggacctcgt ccgtttcgcc cgatgcgccg taggaggtgg cgccgaagga aaggaaggtt
     3181 cccgtgaagc cgagtttgag cagggacgcg gttatgacgg gggcgatgcc ggtcctgacg
     3241 ttgtggtcct cgtaggtgac gaccgagggc agggttgcca gctttgccat cacggtctcg
     3301 tcgacctcgt ccacgcaggg catattcacc accgcgaagg acttcccctt cccggccagc
     3361 ttctccctga tgcccagggc cttcgagaca agccctccat aggtgatgat ggcgccgtcc
     3421 gtgccgtcgg ccatgaggtc catcttcccg tattcgaagg cgtaatcgcc ggcgaaatat
     3481 ggttcgcctt cgttcctgta catcacgggc cagcggttgc ggcccatgcc gatgaagaag
     3541 ttgccccgta cggccgccac gtagcggat
//
LOCUS       DAOPOA010000149         3567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_28410_length_3567_cov_2.857916, whole genome shotgun sequence.
ACCESSION   DAOPOA010000149 DAOPOA010000000
VERSION     DAOPOA010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28410_length_3567_cov_2.857916"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PKJ17_04220"
     CDS             <1..723
                     /locus_tag="PKJ17_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD75207.1"
                     /translation="RGKTAVRGICGAMLFDGDKALKKIGVLSGGERSRVLLGKLLAAP
                     ANLLLLDEPTNHLDMESIDSLIEAINEFDGAVMIVTHSEMILDAVAERLIVFDGNRVG
                     IFDGSYEDFLSRVGWEDEAEGRAGESEGVAAPAKSAIRRESKRLKADIVNERSRAMTP
                     VKTRINDIESAITETERQLARDNEALIAASQKGDGKAIISLSMSIHEARQRIDGLFDE
                     LEVLTLEHDALAREFEQRLNEL"
     gene            complement(852..1997)
                     /locus_tag="PKJ17_04225"
     CDS             complement(852..1997)
                     /locus_tag="PKJ17_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD75208.1"
                     /translation="MMDPYINNDLKDVESAVSEFCAKELDQDHLHKLEDEHRYPRELY
                     EKIGNLGFIGVGFAEDIGGSGYGALGHVMAVKELCRCLPGMGMALSLGYIPGFVVDIY
                     GSDEQREKYVRPMIEGRYVSAISVTEPDSGSDLMSLKTRMEDRGDRYVVNGSKVFTTN
                     AGYADFYALLAKDEDRQSIMIIDRELIEKNGPGRFEINDLGRKMGINTTTSGELVFND
                     FEIPKNNVLGQRGKGLRMVLDGFELGRIVIAAQAFGAMLYGYEKTVGYAKKRKQFGQS
                     IIRFQSVEHDLVDMYVEIEKCRNLLYKAAWLYDNKDPESKKYSSLVKLAIPECAIKVL
                     NRTIDILGGYGYMRDQGLEGALRDIRITSIYEGTSNIQKNTLSRMIF"
     gene            2224..2805
                     /locus_tag="PKJ17_04230"
     CDS             2224..2805
                     /locus_tag="PKJ17_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75209.1"
                     /translation="MPLAFHSLSHGEVAFGFFNIDTDMLLLDVHFFFADDFSAAVSGL
                     ASFEADGPARVNLDAYTLDRGRVGNLMGAIHGIEFTGFIGDVYKRFPFPAEEEKFRQQ
                     PEGFKTRRVIEEMVKPYAGKRTIPIVSDDRRLTVNIGGYLFDGAGFQDLILYVWRGGY
                     PRWRDGLRPAYVMEMMQAVERSRNPLLRGLKRP"
     gene            3044..3235
                     /locus_tag="PKJ17_04235"
     CDS             3044..3235
                     /locus_tag="PKJ17_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HOD75210.1"
                     /translation="MEEKKSKEERFNEARILHKSLDEKLQMLQAKPYLTEDEELEMKL
                     LKKKKLYFKDMMEKIRNEE"
     gene            complement(3219..>3567)
                     /locus_tag="PKJ17_04240"
     CDS             complement(3219..>3567)
                     /locus_tag="PKJ17_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOD75211.1"
                     /translation="LLIFLTGRARRLLRRHRTAADVYRALSGWSKRSGIRRGGSETTR
                     EFSSRLSGAFPSLADEIGSITEAFNRELYGQMTITGEQMALLKANRRRLASPSLWPAR
                     LRTLIAGQVTPRS"
BASE COUNT          731 a         1039 c         1007 g          790 t
ORIGIN      
        1 cggggcaaga cggccgtcag gggtatctgc ggcgccatgc tctttgatgg ggacaaggcc
       61 ctcaagaaga taggtgtgct ctccgggggc gagaggagca gggtgctcct gggcaagctc
      121 ctcgccgccc cggcgaacct cctgcttctt gacgagccca caaaccatct cgacatggag
      181 tccatcgact ccctcatcga ggccatcaac gaattcgacg gcgcggtgat gatcgtgacc
      241 cacagcgaga tgatcctcga cgccgtcgcg gagcggctca tcgtcttcga cgggaacagg
      301 gtcggcatct tcgacggcag ctacgaggac ttcctctccc gcgtcggctg ggaggacgag
      361 gccgagggca gggcagggga gagcgagggc gtcgcggccc cggcgaagag cgccatcaga
      421 agagaatcga aaaggctcaa ggccgacatc gtcaacgagc gttcccgcgc catgacgccc
      481 gtgaagacgc gcatcaacga catcgagtcc gccatcaccg aaacagagag gcagctcgcc
      541 cgcgataacg aggccctcat cgcggcctca cagaagggcg acggcaaggc catcatatcc
      601 ctctcgatgt ccatccacga agccagacag cggatagacg gcctcttcga cgaactcgag
      661 gtcctcaccc tggaacacga cgccctggca cgggagttcg aacagcggct gaacgaactg
      721 tagagatgaa gaaggttcaa gggttcaaga gtaaaagaca aacaggacac cggtgccttc
      781 ggccggtgtg cgaattgtgt tgacagccga cctcccttcg cggtcttacc cttgaaccct
      841 tgaaccgtcc tttagaagat cattctcgac agtgtgttct tctgtatgtt gctcgtccct
      901 tcgtagatcg atgttatccg tatgtccctc agggcgccct cgaggccctg gtcgcgcatg
      961 tagccgtagc ctcccaggat gtcgatggtc ctgttgagga cctttatggc gcattccggt
     1021 atcgccagct tcacgaggga ggagtacttc ttcgattccg ggtccttgtt gtcgtagagc
     1081 caggcggcct tgtacaggag gttgcggcac ttttcgatct ccacgtacat gtccacgagg
     1141 tcgtgctcga cggactgaaa acggattatg gactggccga actgcttgcg tttcttcgcg
     1201 tagccgacgg tcttctcgta gccgtagagc atggccccga aggcctgggc ggctataact
     1261 atcctgccca gttcgaaccc gtcgaggacc atcctcaagc ccttgccgcg ctggccgagg
     1321 acgttgtttt tcgggatctc gaaatcgttg aagacgagtt ctcccgacgt ggtggtgttg
     1381 atgcccatct tcctgccgag gtcgttgatc tcgaaccggc cgggaccgtt cttctcgata
     1441 agttccctgt cgatgatcat tatgctctga cggtcctcgt ccttcgcgag gagggcgtag
     1501 aagtcggcgt accccgcgtt cgtcgtgaag accttcgagc cgttcaccac atagcggtct
     1561 cccctgtcct ccatccgtgt cttgaggctc atgaggtcgc ttccgctgtc gggctctgtg
     1621 acgcttatgg cggagacgta cctcccctct atcatgggcc gcacgtactt ttcccgctgc
     1681 tcgtcgctgc cgtaaatatc gacgacgaac ccggggatgt atccgaggct cagggccatc
     1741 cccatgcctg gcaggcaccg gcacagctcc ttgacggcca tgacgtggcc gagcgccccg
     1801 tatcccgaac cgccgatgtc ctcggcgaaa ccgactccta tgaaccccag gttcccgatc
     1861 ttctcgtaga gttccctggg gtacctgtgc tcatcctcga gtttgtggag gtggtcctga
     1921 tcgagttcct tcgcacagaa ttcgctcacc gcgctctcga cgtccttgag gtcgttattg
     1981 atataagggt ccatcatcct tcaccacctg tcctcgcgat cgatgttttt gactgcctta
     2041 attctcctgc ggatgaccat gttttgcaag gggaaaagga atgttcaaga gttcaagtgt
     2101 tcaaaggttc aaggggaacg accaaaagag agcatctcaa tgcaattact gacaagagca
     2161 actgaccagg cgttatatga tatactggat tgaactcttg aaccatcttt gcccggaggt
     2221 cctatgcccc ttgcctttca ttccttgagc catggggagg tagcgtttgg ttttttcaat
     2281 atcgatacgg acatgctgct cctcgatgtc cacttcttct tcgccgatga cttctcggcg
     2341 gccgtttccg gactggcgtc gttcgaggcg gatggcccgg cgagggtcaa cctcgatgcc
     2401 tacacccttg accggggccg cgtcgggaac ctgatgggag cgattcacgg catcgagttt
     2461 acggggttca tcggggatgt ttacaagcgg tttcccttcc cggccgaaga ggagaagttc
     2521 aggcagcagc ctgagggttt taagacccgc cgcgtcatcg aagagatggt aaagccctat
     2581 gcagggaagc gcaccattcc catcgtcagc gacgaccgcc gtctcacagt gaacatcggc
     2641 ggctacctct ttgacggagc cggttttcag gaccttatcc tctatgtctg gcgcggaggc
     2701 tatccgcgct ggcgcgacgg cctgcgcccc gcctacgtga tggagatgat gcaggctgtc
     2761 gaacgatccc ggaaccccct cctccgtggc ttaaagcgcc cctgattccc gtgaaatgac
     2821 ggatggataa agggaatgac caataaccaa attccaatga ccagacaaac agacaataac
     2881 caataaccaa aaaaacaaca caggcggccc gttctgccct ttttctttgg ttactggaat
     2941 ttggtcaatt ggttatttct gtttcctgat tatctttgga tcgatctatc tatctattct
     3001 ttgttcttta ctccttcccc ctcaccgctt acactatagg cctatggaag aaaaaaagag
     3061 caaggaagag cgtttcaacg aagcccgtat tctccacaag tcgctcgatg aaaagcttca
     3121 gatgcttcag gcgaagccct acctgaccga ggatgaagag ctcgagatga agcttctcaa
     3181 gaagaagaag ctctacttca aggacatgat ggagaaaatc aggaacgagg agtgacctgt
     3241 cccgcgatga gggtcctgag tcttgccggc cagagggagg gactggcgag cctgcgccgg
     3301 ttggccttca ggagggccat ctgttcgccg gtgatcgtca tctgtccgta aagttccctg
     3361 ttaaaggcct ccgtgatgga tcctatctcg tctgccaggg aagggaatgc ccccgaaagc
     3421 cgtgaggaga actcccgggt cgtttcgctt ccgccccgtc gtatgccgct tcgctttgac
     3481 catcccgaga gcgcccggta gacgtctgcc gccgtcctgt gtcgcctgag gagcctgcgg
     3541 gcccgtccgg tgagaaagat gaggagg
//
LOCUS       DAOPOA010000150         3563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_28457_length_3563_cov_2.734322, whole genome shotgun sequence.
ACCESSION   DAOPOA010000150 DAOPOA010000000
VERSION     DAOPOA010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28457_length_3563_cov_2.734322"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..691)
                     /locus_tag="PKJ17_04245"
     CDS             complement(65..691)
                     /locus_tag="PKJ17_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75212.1"
                     /translation="MSYLLLAVTFIVSLLLPASALHAGPVVAVKDFQGSGPSSQLGSG
                     LSSMVETELANSEGLKKCGGSVVEWKRRKDVVDEIERQQGPGFDPKSRAKKGKLLEPT
                     VFVVGDVITSDTDVRWSIKLVDPATGRTIGRHDGSVPADKDSVFEAPREIAENLGEQI
                     CRQGMEYTSPAGRSGNAGTSDSPAGDPSGVLDKVNGVVDSIKGFKKLF"
     gene            920..1417
                     /locus_tag="PKJ17_04250"
     CDS             920..1417
                     /locus_tag="PKJ17_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75213.1"
                     /translation="MTVKVYMRTVLLAALVTMSLGGFMLHLRVHPFSQHSSILVNIVA
                     SLLSILVVPVLFSIRKTVHYGYVLNGFITIVATITMGHFSIHYLPDPLSIGGIFLGTT
                     LPDILIVWGKFFVGKALFDLEVMGYNADVEKKGIAWRYPNLGWWLVHLIAVSAVYWAG
                     NVLWR"
     gene            complement(1663..2958)
                     /locus_tag="PKJ17_04255"
     CDS             complement(1663..2958)
                     /locus_tag="PKJ17_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75214.1"
                     /translation="MTGSSAPRGFRDMLSFPLVEAILGRRSRRFFMGADIPDGVFAYR
                     SERKALPLTDMEKMLVVSACGGNTSWHHMIYRAARYAPHLSNYAGSAGGRVFPSAAGF
                     HTSEIFFTDDDGVYVLQMRDAPACDDRADDGSLSLDAFVDNVRKRVRKVHDSRLGLPS
                     EVPYTEAHNTWVFNKPSTLVVIPVGDLSQHVLLNICYMLQNGLVYYDDINRRPIPGID
                     RFKDIVDANNVWPITFVEQWSLSELTVELGTSCYAGTLMLQAMGLGGWMFNGVDPFAM
                     LGASGKPGVPGLGFRYDEDERWPYPNPTGLTGVMEGYCPPHHHDMRAAVDALCERKFG
                     PGGPFHPDTPGPWKDSRKVRSAAQSHDERFRECVALQAQYVYDTFGKFPGTVPSMFVI
                     MYLQAHHLDLEFYDRFYDPGAYLKTHAMHMADWHPDDPD"
     gene            complement(2972..3538)
                     /locus_tag="PKJ17_04260"
     CDS             complement(2972..3538)
                     /locus_tag="PKJ17_04260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOD75215.1"
                     /translation="MADNKITNGIDVTRLFETIEAIKANPSIAQFKFRATNTWINGTH
                     CRATIRDFYGALKEDDSRPAVDYDMDEPPILLGNNEGRNPVEYLLVALSGCLTTSLIA
                     HASARGIAIRGVRSRYEGDIDLRGFLGVSEDVPVGYQSIRVYFEIDADASREQKEEMV
                     RNARKYSPVFNTLTRSVPVSVQLDKESL"
BASE COUNT          696 a         1050 c         1027 g          790 t
ORIGIN      
        1 gcggaacgga ccgggggatc cagccggttg ttcccgttga gcaggcagac cgcaaaccac
       61 acacctagaa cagttttttg aatcccttga tcgagtccac taccccattc accttgtcca
      121 gcaccccgga agggtcacct gccggggagt ccgatgtccc ggcgttgccc gatctgcccg
      181 ccggggacgt gtactccatg ccttgacgac agatctgctc ccccaggttc tctgctattt
      241 cacggggcgc ctcgaacacg ctgtccttgt ccgcgggaac acttccgtcg tgacgcccga
      301 tggtgcgccc cgtggccggg tcaaccagct tgatcgacca gcgcacgtct gtgtcactgg
      361 tgatgacatc ccctacgaca aaaacagtcg gctccaggag cttccccttt ttggcgcgac
      421 tcttcggatc aaatccgggt ccctgctgac gctcgatctc atcaaccacg tccttgcgtc
      481 tcttccactc aaccaccgag ccaccgcatt ttttcagccc ttctgaattc gcgagttcgg
      541 tctccaccat ggaagagagt cccgatccca gctgggacga aggcccgctt ccctgaaagt
      601 ccttcacggc caccaccggt cctgcatgga gcgccgacgc cggcaacagg agactgacta
      661 tgaatgtgac cgctagtaac aagtagctca tgctctcacc tcattctgaa gttaaactga
      721 tcggagtaga ccaattaatg tcgcttatac aaaagaggta tgtcaaggaa aagggggctg
      781 ccgcacaagg gttcccacat accccggttg acaggagggt ctggaatgct tctattggtt
      841 gtatgctgga taacccgaca gggaacagtc gggtgaggaa cgcggtgggc cgtgatggag
      901 accgtgggga ggagctgtga tgacggtaaa ggtttacatg aggactgtcc ttctcgcggc
      961 cctcgtcacg atgagcctcg gtggttttat gcttcatctt cgcgtacatc cattcagcca
     1021 gcacagctcg attcttgtca acatcgtggc cagcctcctg agcatccttg tcgttccggt
     1081 gcttttttcc atcaggaaga cggtgcatta cgggtatgtg ctgaacggtt tcatcacgat
     1141 agttgccacg atcaccatgg gccatttctc gatccactac ctgcctgacc cgctgagcat
     1201 cggcggcatt tttctcggga cgacccttcc cgatatcctg atagtctggg gaaagttctt
     1261 cgtgggcaag gcgctgttcg atctggaggt catgggatat aatgccgacg tcgagaagaa
     1321 aggtatcgcc tggcggtatc cgaacctcgg ctggtggctg gtccatctta ttgccgtttc
     1381 ggccgtgtac tgggccggca acgtgctgtg gagatgacat gaacgctttc agggcttttt
     1441 ccctcggctg gtggatactt cacgtcatag ccatcgcctt tttcctgtgg ctcggccatg
     1501 ccaccacgtt ctgaaacagc ttgggacacc ttctcattct cacattcgtg acacgcgggg
     1561 cagagcgacc ctccggcgaa tctttcctga tcgtgcgtct tgcgccaggc aatgggggcg
     1621 cagggctcgc gaaggggcag atggaaaggg ccgcggcgtt attcagtcgg ggtcgtccgg
     1681 gtgccagtca gccatgtgca tggcatgcgt cttcagataa gcccccgggt cgtagaacct
     1741 gtcatagaac tccaggtcga ggtgatgcgc ctgcaggtac atgatgacga acatcgaggg
     1801 caccgtgccg gggaacttgc caaaggtatc ataaacgtat tgggcctgca gggcgacgca
     1861 ctccctgaat ctttcgtcgt ggctttgtgc cgctgaacgc accttgcggc tgtccttcca
     1921 cggtccgggg gtgtcgggat gaaagggccc ccccggaccg aacttgcgct cgcacagtgc
     1981 atccacggcg gcccgcatat catggtggtg gggcgggcag tagccttcca tcacccccgt
     2041 gagtcccgtg gggttgggat agggccagcg ttcgtcctca tcgtaccgga atcccagacc
     2101 gggcacgccg ggtttgccgc ttgcgccgag catggcgaag ggatcgactc cgttgaacat
     2161 ccacccgccg aggcccatgg cctggagcat cagagtgccg gcatagcagc ttgtcccgag
     2221 ttccacggta agctccgaca gcgaccattg ctccacaaag gtgatgggcc agacgttgtt
     2281 tgcatccacg atgtccttga accggtcaat gcccgggatg ggacgcctgt tgatgtcatc
     2341 gtagtatacc agcccgttct gaagcatgta gcagatgttg agcaggacgt gctgtgaaag
     2401 gtccccgacg gggatcacga ccagggtgga cggcttgttg aacacccagg tgttgtgcgc
     2461 ctcggtgtaa ggcacctcgg atggaagccc aagccgcgaa tcatgcactt tgcgcacccg
     2521 tttcctgacg ttgtcaacga acgcgtcgag gctcagcgat ccatcatcgg cgcggtcgtc
     2581 acaagccggc gcgtcgcgca tctgaagcac atacacgccg tcgtcatcgg tgaagaaaat
     2641 ctcgctcgtg tgaaagccgg ccgctgaagg aaacacccgc ccgccggcag acccggcgta
     2701 attggaaagg tgcggggcat atcgggccgc ccggtagatc atatgatgcc aggatgtgtt
     2761 tccgccgcag gcggatacca ccagcatttt ctccatatcg gtcaacggca gcgccttccg
     2821 ctccgacctg tacgcgaata ctccgtccgg gatgtccgct cccatgaaga acctccggga
     2881 ccggcggccc aaaatggctt ccacgagggg aaaagacagc atgtcccgaa aaccgcgagg
     2941 ggctgatgat cctgtcatga atctctcctg atcaaagact ctctttatcg agttgcacgg
     3001 aaacgggaac ggacctcgtg agcgtattga acaccggtga atacttccgg gcgttgcgga
     3061 ccatctcctc cttctgttcc ctggaagcgt ccgcgtcgat ctcaaaatag acccggatgc
     3121 tttgataccc gaccggcacg tcttccgata cccccagaaa accgcggagg tctatgtcgc
     3181 cttcatatct ggaccgcacc cctcgtattg cgatccctct ggcagaggcg tgggcgatca
     3241 ggcttgtggt gagacaaccg gagagggcga cgaggagata ttcgacgggg tttctcccct
     3301 cgttgtttcc caggaggatc gggggctcat ccatgtcgta atccacagcg ggccgggagt
     3361 cgtcctcctt cagggccccg tagaagtccc tgatcgttgc ccggcaatgg gttccgttga
     3421 tccatgtgtt ggtcgcgcga aacttgaatt gcgcaatgga ggggttggcc ttgatggctt
     3481 cgatcgtctc gaataatcgg gttacatcga taccgttcgt tattttattg tccgccattt
     3541 cgattgcctc cttttttttc agg
//
LOCUS       DAOPOA010000151         3552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_28571_length_3552_cov_3.983986, whole genome shotgun sequence.
ACCESSION   DAOPOA010000151 DAOPOA010000000
VERSION     DAOPOA010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28571_length_3552_cov_3.983986"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1460)
                     /gene="glgC"
                     /locus_tag="PKJ17_04265"
     CDS             complement(231..1460)
                     /gene="glgC"
                     /locus_tag="PKJ17_04265"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOD75216.1"
                     /translation="MRSRIMHNAVAFVLAGGAGERLHPLTRDRAKPAVVFGGKYRIID
                     FTLSNCVNSGMRKIFVLPQYKSHSLIEHTRDAWSILNPELGEFVSNLSPQMRVGEDWY
                     RGTADAVYQNLYHLRQVDADHVVVLSADHVYKMDYSHFVRYHQSKRSDMTISGIEVDI
                     SEASRFGVIHTGSDSRVIGFEEKPAEPRPMPGVPDKAFVSMGVYVFKRDVLIDMLTRD
                     ARNEGSFHDFARDLIPFMYPRNRVFVYRFGVGKGRDSAYWRDIGTIEAYWQANMDLAS
                     VTPEFNLYDRDWPIRTYEGQYPPAKTVFADETGGRAGKALDSIICSGVIISGGTVRNS
                     VLSPGVRVNSYAEVSQSILFHNVVVGMRAKVRRAIIDKGVRIPDGMRIGYDLEKDRER
                     FFVSDEGIVVIPKGEVL"
     gene            complement(1528..2211)
                     /gene="rsmI"
                     /locus_tag="PKJ17_04270"
     CDS             complement(1528..2211)
                     /gene="rsmI"
                     /locus_tag="PKJ17_04270"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOD75217.1"
                     /translation="MDSQGILYVIATPIGNLGDITLRAIDVLKEVDLVVAESTSRALK
                     LFNHYGIKNAILGISSYNEERRSAGIIRSLKAGRKVAIITAAGTPCISDPGSILVSKC
                     VAEDIEVRAIPGASAVSALVSVSGLFADRFIFYGFLPLKKGKRKRALEELSSFPYPIV
                     FYESPRRIEETLGYVLDIMGDRRMVAVKEMTKLFERTYRGTVSEVIEELSGEEKRGEY
                     AFMVAGSER"
     gene            2276..2851
                     /locus_tag="PKJ17_04275"
     CDS             2276..2851
                     /locus_tag="PKJ17_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOD75218.1"
                     /translation="MSIKVALIAGLCLLPLLAYPAIYTYVDDSGTSHFTNILPVGKKF
                     RVVISERIRHVVARVFNNTTYDGIIERHAQTHGIDPSLVKAVMKAESNFNPNAMSHKG
                     AQGLMQLMPDTARLMKVDNPFDPEENIRGGTKYLKYLGETFGGNLDLVLAAYNAGPAR
                     VKEYNMSIPPYDETRTYVQRVKTYYNSFKKN"
     gene            2851..3405
                     /gene="pgsA"
                     /locus_tag="PKJ17_04280"
     CDS             2851..3405
                     /gene="pgsA"
                     /locus_tag="PKJ17_04280"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOD75219.1"
                     /translation="MKAKDEQLPLWTLPNRLCILRILFIPLIIIFIEKGFFYVAFVLF
                     ILAGITDGLDGFVARRFKLTSKLGLYLDPIADKLLVSSVLITLTYHNLVPLWVTILLV
                     CREFIINGLRSFYALEGITIYPSFAGKLKTTLQIVAISFLLFKHPLAQQIGLYVIYAA
                     LLFSMYSAVKYITAIFRPKKIVDG"
BASE COUNT          827 a         1015 c          907 g          803 t
ORIGIN      
        1 ggacatacct ctcggtccgg gacaacctgg caacaagcaa aggcacggta gtggccaacc
       61 tcgtaaggct ctacaaagcc ctgggtggcg gctggacccc aaatagccca cagccggaag
      121 ccgcgcagac gacgaaaaca gagggatgag cttgaaccgc ttgaaccgtt tgagctgctt
      181 gaacgtgaga aactaaaaga cctccgtatc gaattagagg gttatacagg ctagaggacc
      241 tctcctttgg ggatcaccac tattccttcg tcggagacga aaaacctctc gcggtccttt
      301 tccaggtcgt agcctatccg catgccgtcg ggtatgcgaa cgcccttgtc tatgatggcc
      361 cttcttacct tcgccctcat gccgacgacc acattgtgga agagaatgga ctgactgacc
      421 tcggcgtagc tgttgacgcg aacaccgggt gaaagaacgg aattgcgcac tgtgccaccg
      481 ctgatgatga ctccgctgca gatgatcgaa tcgagagctt tgcccgccct gccccctgtc
      541 tcgtctgcaa aaacggtctt cgccgggggg tactgcccct cgtaggtcct gataggccaa
      601 tcccggtcgt acagattgaa ttccggggta accgaagcaa ggtccatatt agcctgccag
      661 tacgcctcga tcgtaccgat atccctccag tatgcactgt cgcggccctt cccgacgccg
      721 aaccggtaga caaaaacgcg gttacgggga tacatgaagg gtatgaggtc ccgcgcaaag
      781 tcatggaaag atccctcatt ccgggcgtcc ctcgtgagca tgtcgataag gacgtcacgc
      841 ttgaagacat agacccccat ggaaacgaag gccttgtcgg gaactccggg catggggcgc
      901 ggttccgccg gcttttcctc gaaacctatg acccgcgaat cggagcctgt gtggatgacg
      961 ccgaaccgcg aggcctccga gatatcgacc tcgataccgg agatggtcat gtccgatctc
     1021 ttgctctggt gatatctcac gaaatggctg taatccatct tatacacgtg atctgcggaa
     1081 aggacgacca catggtcggc atccacctgc ctcaggtgat agaggttctg atataccgcg
     1141 tcggcggtgc ccctgtacca atcctctccc accctcatct ggggcgagag gttggagacg
     1201 aactcgccca actcgggatt aaggatgctc caggcgtcgc gtgtgtgctc aatgagggaa
     1261 tgcgatttgt actggggaag cacgaatatc tttctcatgc ccgaattgac gcagttgctc
     1321 agcgtgaagt cgatgatgcg gtatttgccg ccgaatacca cggcgggttt cgccctgtcc
     1381 cgcgtgagcg gatgcaacct ctcgcccgca ccgccggcga gaacgaaggc gaccgcgtta
     1441 tgcatgatcc tcgatctcat gggcctgtca acattatgat gggctgaagg cccgtattca
     1501 acaaaaatcg aaaaggatcc gtgcggatca tctttcgctt ccggcgacca taaaggcgta
     1561 ctctcccctt ttctcctctc ccgagagttc ttcgatgacc tcactcacgg tgccccgata
     1621 ggttctctcg aagagctttg tcatctcttt caccgcgacc atcctcctgt ctcccatgat
     1681 gtcgagaaca tatccgaggg tctcctcgat gcggcgcggc gactcgtaga agacaatcgg
     1741 gtacggaaac gaagaaagct cttcgagggc cctcttccgt tttcctttct tgagaggaag
     1801 gaaaccgtag aagatgaagc ggtccgcaaa aagacccgac acagagacca gcgccgaaac
     1861 cgctgaagct ccgggtatgg cacggacctc gatgtcctcc gcgacgcact tgctgacaag
     1921 aatactgcca gggtcggaga tgcagggagt cccggcagcc gttatgatcg cgaccttcct
     1981 gccagccttg aggctgcgga ttattcccgc ggatctcctc tcctcgttgt agctgctgat
     2041 acccaggatg gcgttcttga tgccataatg attgaacagc ttcaaagcgc gcgatgtgct
     2101 ttccgccacg acgagatcga cctctttcag aacatctatc gccctgaggg tgatgtcccc
     2161 gaggttgccg atgggagttg cgatgacata gagaatccct tgcgaatcca tgactatcaa
     2221 gcatattaca acccaaaact cttgacaagg ccaaaaaagt ttaagagaat taaatatgag
     2281 tatcaaggtt gcgctcatag cgggtttgtg cctgttgccg cttctggcat acccggcgat
     2341 ctatacctac gtggatgata gcggcacctc tcacttcacg aacattcttc ccgtcggtaa
     2401 gaaattcagg gtcgtgatct ctgaacgcat aagacacgtc gttgcgcggg tattcaacaa
     2461 caccacctat gacgggatca tcgaacgcca cgcgcagacg cacgggatag atccctcgct
     2521 cgtcaaggcg gttatgaagg ccgaatcgaa tttcaacccc aatgccatgt cgcataaagg
     2581 cgctcagggc ctgatgcagc ttatgccgga cacggcacgt ttgatgaagg tagataaccc
     2641 cttcgatccc gaagagaaca tccggggcgg gacgaaatac ctgaaatatc tgggcgagac
     2701 gttcgggggc aatctcgacc tcgtgctggc ggcatacaat gccggtccgg cgcgggtcaa
     2761 ggagtacaac atgagcatcc cgccttacga tgaaacacgc acatacgttc agcgggtcaa
     2821 aacctactat aactcgttca agaagaattg atgaaagcca aagacgaaca gctccccctc
     2881 tggacgctac ccaaccggtt atgcatcctc aggatacttt ttatccccct gatcataatc
     2941 ttcatagaga aagggttctt ctacgtcgcc ttcgtccttt ttatactggc cggcataacg
     3001 gacgggctgg acggtttcgt ggcgcgaagg ttcaagctca cgtcaaagct cggtctctac
     3061 ctcgatccca tcgccgacaa gctgctcgtt tcatcggtgc tcatcacgct cacctatcat
     3121 aacctggtgc ccctatgggt gaccatcctc ctcgtgtgca gagagttcat catcaacggg
     3181 ctgaggtcct tctacgccct tgaaggcatc accatctacc cttcttttgc cggaaagctc
     3241 aagaccacgc tccagatcgt ggccatatcc ttcctcctct tcaagcatcc cctcgctcaa
     3301 cagataggcc tctacgtaat ctatgccgca ttgctcttca gtatgtactc ggcagtgaag
     3361 tatataacgg caatattcag accgaagaag atagttgatg ggtgatgccg gtctcaagtc
     3421 tcagctttca aatttcaagc cttgagttcg acactacaaa ttccgtacta tatcttttca
     3481 tagtgcctaa atgatgtatt tttgtgcgat ggatgtgttc gtcgtctttc aaccttgaac
     3541 tcttgaaccc tg
//
LOCUS       DAOPOA010000152         3552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_28575_length_3552_cov_3.090935, whole genome shotgun sequence.
ACCESSION   DAOPOA010000152 DAOPOA010000000
VERSION     DAOPOA010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28575_length_3552_cov_3.090935"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..511
                     /locus_tag="PKJ17_04285"
     CDS             122..511
                     /locus_tag="PKJ17_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOD75220.1"
                     /translation="MDTRPIKVLVAKPGLDGHDRGAKVVAHALKEAGMEVIYTGLHRT
                     VDQIIRIAIQEDVDVIGLSIMSGAHIPITEKLMKKAREEGIDDKMVVVGGSYPAGTSR
                     SSGSWGPEGCSPVGHRSRRSRASSMKT"
     gene            534..2162
                     /locus_tag="PKJ17_04290"
     CDS             534..2162
                     /locus_tag="PKJ17_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOD75221.1"
                     /translation="MGVAKYTKDEKDRITDVTLQSGIHVKACYGPADLESVGFSYEKD
                     LAEPGQYPFTRGIHAEGFRSRAWTTRQYTGFGTPEESNERFKLMISHGQTGLNVAFDL
                     PTQMGYDSDDPMAEGEVGRVGMAIDSLRDFEIAFKGINLEKIGSGLTINAVASIMLAM
                     YQAVAEKYGFDPKKISATPQNDILKEMIGRGSWIFPVEPAVNLIGDTIEYSMNVLPRT
                     NPVSICGYHIRESGATPAQEIGYAILIANAYIDNVLKRGYDVDDFVGRFSFNLNVFGN
                     IWEQVAKFRAARKLWARNLKEKYGVKKDSNMYLRGLFGGGGYGLTKAQPENNIMRGAY
                     YALSAALAGAQTIALCSYDEAYTIPTPHSAIISLRTLQLLMDEIGLRDTVDPLAGSYF
                     IETITKEMEAKIEEEMGKIEKVGGIVQAVATGYVQRLVSQQAYEYEKGLQSGELKKVG
                     LNIYTEGEPMEVELHEYTGESAEKQIEALKQLRRERNDREVTRTLKELETATRAGKNV
                     MPCLVEACKAYATVGEMARVFRDVFGEFEEPGLF"
     gene            2301..>3552
                     /locus_tag="PKJ17_04295"
     CDS             2301..>3552
                     /locus_tag="PKJ17_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOD75222.1"
                     /translation="MQDRYYRLGCDIGGTFTDFVLLDDQTGEIRTGKCLTTPRDPSDA
                     VEEGIRGLEKTTPDFVGKLDELIHGTTLVINSIIERKGAKTGLITTKGFRDILEIGRE
                     IRYAPYDIFAEFPQPLIPRRFRLEVDERVRSDGTILKPLDPEDAKRTVRALLDMGVES
                     IAVCLINSFENPVHERMIEEIIHAEAPEVSTSISYHVLPQIKEYERTSTTVTNAYVKP
                     LTGRYLSKLSGRLESIGFAGKLFIMLSSGGVTSVDTAAQFPVRIIESGPTAAVIAGQY
                     YGKHFNIPEMFCFDMGGTTAKSCLIQKGVAGVVPTFEVGRVQRFMKGSGLTIQVPVVD
                     LMEIGAGGGSIAKVSKLGTLQVGPESSGADPGPICYGRGGQDPCVTDADLLLGYLDEN
                     YFLGGTMKLDKEGARRGVEERIAKP"
BASE COUNT          822 a          950 c         1150 g          630 t
ORIGIN      
        1 agggttgagg cgctggtcag gaagcacatc ctgcggggga tggatatcat aattcaggag
       61 ctcgacgcgg gcaagtcggt gtagcggcga aagagcggaa gaaacaagca aggaggtaca
      121 attggacacg aggcccatca aagtcttagt tgcgaagccg ggactggacg gacacgaccg
      181 cggggcaaag gtggttgccc acgcgctcaa ggaggccggc atggaggtga tctacaccgg
      241 gcttcaccgg acggtggatc agatcatcag gatagccatc caggaggatg tggatgtcat
      301 cgggctgtcc atcatgagcg gagctcacat tcccatcacg gagaagttga tgaagaaggc
      361 ccgtgaggaa gggatcgacg acaagatggt cgttgtcggg gggtcatacc cggcagggac
      421 atcccgaagc tcagggagct gggggccaga ggggtgttcc ccggtgggac accgttctcg
      481 gagatcacga gcttcatcaa tgaaaacgtg aagaagtcct gaggaggcat gcaatgggag
      541 tggcgaaata taccaaggat gagaaggaca ggatcaccga cgtgaccctg cagtcgggga
      601 tacatgtcaa ggcgtgctat ggtcctgcag acctcgagag cgtgggtttt tcctacgaga
      661 aagaccttgc cgagccgggg cagtacccgt tcacccgggg gatacatgcc gagggtttcc
      721 gcagcagggc gtggacgacg cggcagtata ccgggttcgg cacacccgag gaatcgaacg
      781 agcgtttcaa gctcatgata tcccatggcc agacgggcct caatgtcgcc ttcgaccttc
      841 ccacgcagat ggggtacgat tccgacgacc ccatggcgga gggcgaggtg ggcagggtcg
      901 gcatggccat cgattctctc agggatttcg agatagcctt caaggggatc aacctcgaaa
      961 agatcggttc cggtcttacg atcaacgccg tcgcctcgat catgcttgcg atgtaccagg
     1021 ccgtggcgga gaaatacggt ttcgacccga agaagatctc ggcgacgccg cagaacgaca
     1081 tcctgaaaga gatgatcgga aggggctcct ggatctttcc cgtcgagccc gccgtgaacc
     1141 tcatcggtga taccatcgaa tattccatga acgttctgcc ccgcacgaac cccgtgagca
     1201 tctgcggtta ccacatcagg gagtcggggg cgacgcccgc ccaggagata ggctatgcca
     1261 tcctcatcgc caacgcctac atagacaacg ttctgaaacg ggggtacgac gttgacgatt
     1321 tcgtcggccg tttttccttc aacctcaacg tcttcggcaa catctgggag caggtcgcaa
     1381 agttcagggc ggcgcgcaag ctctgggcgc ggaacctcaa ggagaaatac ggggtcaaga
     1441 aggactcgaa catgtacctc aggggtctct tcggaggagg cggctacggt ctcaccaagg
     1501 cgcagcccga gaacaacatc atgcgcggcg cctattacgc cctttccgcc gctctcgccg
     1561 gggcccagac gatcgccctg tgcagctacg acgaggcgta caccatcccg acgccccatt
     1621 cggcgatcat atcccttcgc accctccagc ttctcatgga cgagatcggc ctgagggata
     1681 ccgtagaccc gctggcgggc agctatttca tcgagaccat cacgaaggag atggaggcga
     1741 agatagaaga ggagatgggg aagatcgaga aggtcggcgg tatcgtgcag gcggttgcca
     1801 ccggctacgt gcagcgactt gtatcacagc aggcgtacga atatgaaaag ggattgcagt
     1861 ccggggaact gaaaaaggtg ggactcaaca tctacaccga gggcgaaccg atggaagtgg
     1921 agcttcacga gtacacggga gagtccgcgg aaaaacagat cgaagccctg aaacagcttc
     1981 gccgggagcg caacgatcgg gaagtgacac gaaccctcaa ggaactggaa acggcgacga
     2041 gagccggaaa gaacgtcatg ccctgtctcg tggaggcgtg caaggcgtac gcgacggtgg
     2101 gtgagatggc cagggtcttc agggatgtat tcggagaatt cgaggagccg ggtctctttt
     2161 agggcaaggg ggcaatggaa aagggagccc ggaccgttca cggaggtccg ggcattcccg
     2221 gtgagttccg tatctgattt tgtttttcaa acggttcaaa cggttcaaat ggtttgaacg
     2281 gttctcaaaa gggggatcac atgcaagaca ggtattaccg gctgggttgc gatatcggag
     2341 gcaccttcac tgattttgtc ctgctcgatg accaaacggg agagataagg accggcaagt
     2401 gcctgaccac gccccgggac ccgtcggatg ccgtggaaga ggggatacgg ggcctcgaga
     2461 agacgacccc cgatttcgtc gggaaactgg acgaactcat tcacgggacg acgcttgtca
     2521 tcaattccat aatcgagaga aagggggcga agacgggcct catcacgacg aaggggttca
     2581 gggatattct ggagataggc cgcgagatcc gctacgcgcc gtacgatata ttcgcggaat
     2641 tccctcagcc gctgatcccc aggaggttcc gtctggaggt ggatgagagg gtacgcagcg
     2701 acgggaccat cttgaagccc ctcgacccgg aagacgccaa aaggacggtg agggcgcttc
     2761 tcgatatggg tgtcgagtcc atcgccgtgt gccttatcaa ctcctttgag aacccggtcc
     2821 acgagcgcat gatcgaggag atcatccacg cggaggcccc ggaggtctcc acgtccattt
     2881 cctaccacgt ccttccccag ataaaggagt atgagaggac gagcaccacc gtgaccaacg
     2941 cgtacgtgaa acccctcacg ggacgatacc tgtcgaaact ttcgggacgg cttgagtcca
     3001 taggtttcgc gggtaagctt ttcatcatgc tgtcaagcgg aggtgtcacc tccgtcgata
     3061 cggccgcgca gtttcccgtt cgcatcatcg agtcggggcc gacagccgcg gtcatcgccg
     3121 gccagtacta cgggaagcat ttcaacatcc ccgagatgtt ctgcttcgat atgggcggta
     3181 cgacggcgaa atcgtgcctc atccagaaag gggtggccgg tgtcgttccc accttcgagg
     3241 tgggaagggt ccagaggttc atgaagggga gcgggctcac catccaggtc cccgttgtcg
     3301 acctcatgga gataggggcc ggtggcggca gcatcgcgaa ggtgagcaag ctggggacgc
     3361 tgcaggtggg gcctgaaagc tccggcgcgg accccggacc tatctgctac ggcaggggag
     3421 gacaggaccc ctgtgtgacc gatgccgacc tcctgctggg gtacctcgac gagaactact
     3481 ttctcggggg cacgatgaag ctcgacaagg agggggcccg ccgcggtgta gaggagagga
     3541 tcgcgaagcc gc
//
LOCUS       DAOPOA010000153         3550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_28595_length_3550_cov_3.688412, whole genome shotgun sequence.
ACCESSION   DAOPOA010000153 DAOPOA010000000
VERSION     DAOPOA010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28595_length_3550_cov_3.688412"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..735)
                     /locus_tag="PKJ17_04300"
     CDS             complement(55..735)
                     /locus_tag="PKJ17_04300"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOD75223.1"
                     /translation="MENNGTAWVDLSISPAYRSRYPGVAFGSTLISDCLPLSDAAGFD
                     QHRRGLLRKMRKRETLAGISTRIDLYDRFFRSFGFECPLPGHLKRTVLSGFPRYNLMV
                     DAHFMAEMCAGILVAVTDHDRIEGRLTLDVARRGEVCAGMGGRRFTTIEGEIVLRDER
                     DIICVLCQGADEKTRVRDDTRNVLFYSYAVPGVEGVYLREGLSVAAEVMSRFGGGNVK
                     GLMVFEGT"
     gene            1017..1331
                     /locus_tag="PKJ17_04305"
     CDS             1017..1331
                     /locus_tag="PKJ17_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75224.1"
                     /translation="MDMIRKAVFMGLGAISMTKDKAEELVDDLIKRGEVASAERFKTV
                     DKLLKEADKQEKEFQHKVSAAVQKVIVEMGLPTRKDLEEIGKTLKKIEAKISSTEKKD
                     AG"
     gene            1335..3014
                     /locus_tag="PKJ17_04310"
     CDS             1335..3014
                     /locus_tag="PKJ17_04310"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOD75225.1"
                     /translation="MPIFRARETYRDLKRYSQIASVLAHHGFTEVLDRLKARYRAPRK
                     RKPLIKERVAEGPPIPVRLRLVFEELGPTFIKLGQILSSRPDILPPDYIKELSKLQDD
                     VPPFPFEEARTLLETQLRHPLRELFSSFEEKPVAAASLAQVHRARTPAGEEVAVKIQR
                     IGIEEVIETDIRILREFAGLAERHVPESRYFGPLELVEGFARTIRHELDFVREGRNID
                     RFKAHFISTPTVYIPKVHWDLTSSKVLTIEFITGIKISDVDRLTAAGLDRKTIALNGA
                     NLTLEEIFELRRFHADPHPGNIFVLDNNVIAPVDFGMTGTIDEETSEQMTGIFVAVMQ
                     KDVDTLVGLLRTIGIVGEPYDDRAFKRELHDLFDRYYGVPLNRLDIGTITRELMTIVH
                     RHKLRLPSEFAMIGKALLISEGVGRLLDPEFNIIETARPYARRILLRRFDPARQLRGL
                     SRVADEVIALLRALPADTREILTMMRKGKLAMRFEHRGLELLTDELDRSSNRISFALV
                     IAALIIGSSLIFQTGVGPTVFGYPVLGLGGFLLASILGVWLLIGIMRSKRL"
     gene            complement(3113..3361)
                     /locus_tag="PKJ17_04315"
     CDS             complement(3113..3361)
                     /locus_tag="PKJ17_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75226.1"
                     /translation="MAIFRVTYRNALNSEGEPFELPADKLDTNGARIDCYGYVEREIR
                     EGDNFSRDVYEYDIPDNDADRFIEGLKRTPNVMDYKRQ"
BASE COUNT          736 a         1069 c         1010 g          735 t
ORIGIN      
        1 ggagcaggtc tcgacgatgt cctgtcccat cagggctccc tggggaagcc catctcaggt
       61 cccttcgaac accataaggc ccttcacgtt cccgccacca aagcgggaca tgacctccgc
      121 ggccacgctc agtccttccc tgagatagac cccctctacg ccgggcaccg cataggaata
      181 gaaaagaacg ttgcgcgtat cgtcccgaac ccttgtcttc tcatccgccc cctggcaaag
      241 gacacagatg atgtcccttt catcccggag cacgatctcc ccctcgatcg tcgtgaaccg
      301 gcggcctccc attccggcgc acacctcccc gcgccgggcc acatcgagcg tgagcctccc
      361 ttcgatacga tcgtggtccg tcactgccac gaggatgccg gcgcacatct ccgccatgaa
      421 gtgagcgtcc accatcaggt tgtatctcgg gaagccgctc aagaccgttc tcttgaggtg
      481 cccgggcagg gggcactcaa agccgaagct ccggaagaat ctgtcgtaga ggtcgatgcg
      541 cgtcgatatg ccggcaagtg tctcgcgctt tctcattttt ctgaggagac cccgcctgtg
      601 ctggtcaaac ccggcggcgt cgctcaaagg caggcagtcg gatatgagcg tcgatccgaa
      661 agcgacaccc gggtacctgc tccgataagc gggggagatc gaaaggtcca cccacgccgt
      721 cccgttgttt tccacttccc acctcctatg gtcttcgatc ccttcccatc gacaacgaat
      781 tgtcacgccc ggaacgtgcc tacattatac cctgttttcg accacttacc acaaagatcc
      841 gctgatcggt cagacgcaat gacctggagc gtcacgttcc gtgacttgaa atcggccgtt
      901 cggtggccta atatattctt taagctgagg agccgcgcgc gctgaccgga gattccgcag
      961 ttcccgcaga agaaggggcg tttccagccg gcggcgcctt cagcaaaggg ggtattatgg
     1021 atatgatacg caaagcagtg ttcatgggac ttggcgccat cagcatgaca aaggacaaag
     1081 ccgaagagtt ggtcgacgat ctcatcaagc gcggcgaagt ggcaagcgcg gagaggttca
     1141 agaccgtcga caagcttctc aaggaagcgg acaaacagga gaaggaattt cagcacaagg
     1201 tgtccgcggc cgtacagaag gtgatcgtcg agatgggtct tcccacgcgc aaagacctgg
     1261 aggagatcgg caagacgttg aagaagatcg aggcgaagat atcatccacg gaaaagaagg
     1321 atgcaggtta gcaggtgccc atctttcgag ctcgtgaaac gtaccgggac ctgaagcggt
     1381 acagccagat cgcctctgtc cttgcccacc atggcttcac ggaggtcctg gacagactga
     1441 aggcgcgata ccgcgctcca cggaagcgca aaccgctcat caaagagcgt gttgccgaag
     1501 gacctcccat cccggtgcgg ctccgtctcg tcttcgagga actgggtccc accttcataa
     1561 agctgggtca gatactgagc agccgccccg atatactgcc gcccgactac ataaaggagt
     1621 tgagcaagct tcaggatgat gtccctccct tccctttcga ggaagccaga actctcctgg
     1681 agacgcagtt gaggcacccg ctccgtgagc ttttttcatc cttcgaagag aaaccggtcg
     1741 cggcggcttc cctcgcgcag gtccaccgtg ccaggacacc ggcaggtgaa gaagtggcgg
     1801 tcaagatcca gcggatcggt atagaggagg tcatcgagac cgacatccgc atactcaggg
     1861 agttcgccgg cctcgccgag cggcacgtcc cggagagccg gtatttcggc cccctggagc
     1921 ttgtggaagg ttttgccagg accatccggc acgaactcga tttcgtcagg gaaggacgga
     1981 acatcgaccg cttcaaggcc catttcatca gtacgccgac ggtctatatt ccaaaagtgc
     2041 actgggacct gacgtcgtcc aaggttctga cgatagagtt catcacggga ataaagatat
     2101 ccgacgtcga ccgtctcacg gccgccggcc tcgacaggaa gaccattgcc ctgaacgggg
     2161 ccaatctcac cctcgaggag atattcgagc tccgtcgttt tcacgccgat cctcaccccg
     2221 gcaacatctt cgtcctcgac aacaacgtga tagcgcccgt tgacttcggc atgacgggga
     2281 ccatcgatga agagacctcc gagcagatga ccggcatatt cgtggccgtc atgcagaagg
     2341 atgtggacac tctcgtcggg ctgttgcgaa cgatcggcat cgtcggagaa ccgtatgatg
     2401 accgcgcctt taaacgtgag ctgcacgacc tcttcgaccg ttattatggc gtgcctctga
     2461 acaggctcga tatcgggacc atcaccaggg aactcatgac catcgttcac cgccacaaat
     2521 tgaggctacc gtcggaattt gcgatgatag gcaaggcgct tctcatcagc gagggcgtcg
     2581 ggaggcttct cgaccctgaa ttcaacatca tcgagacggc gcggccctat gcgagaagga
     2641 tcctcttgcg cagattcgac ccggcgaggc agttgcgcgg tctttccagg gttgccgacg
     2701 aggtcatcgc gttattgagg gctttgccgg cggacacgag agagatactc accatgatgc
     2761 ggaaggggaa gctggccatg cgcttcgaac atcgggggct ggagcttctc actgacgaac
     2821 tcgaccggtc gagcaaccgc atcagttttg ctttggtgat agcggccctc attatcgggt
     2881 cctctctcat cttccaaaca ggggtgggtc cgaccgtctt cggttacccc gttctgggcc
     2941 tcggcggctt tctcctcgcc agcatcctcg gggtgtggct gctcatcggc atcatgaggt
     3001 caaagaggct ttgaaggcgg cttgaggggc ttgaattgct tgagtcgctt gacggtcggc
     3061 gggtacgtac gccgggtctc aaggtccctt cagaagaccg ggatcgctcc cgctattgtc
     3121 ttttgtagtc catgacgttg ggtgttctct tgagaccctc gatgaaacgg tcggcgtcgt
     3181 tgtcggggat gtcgtattcg taaacatcac gggaaaagtt gtcgccttcc ctgatctccc
     3241 gttccacgta tccgtagcag tcgatcctcg cgccgttcgt gtcgagtttg tcggcgggga
     3301 gttcgaaggg ttcaccctcg ctgttgagag cgttcctgta tgttaccctg aatatggcca
     3361 tctgaacctt ccctccttac ccgtaatgac cgcccgcccc tatggcaact ccctcagaag
     3421 ttccaccgcc tgggcaagaa gggtcacggt ttgttccatg tagcctccga cgccgggcgg
     3481 agtggcggtg tcgctcgccg ttctcttgac gctatcgctt accgcctgga gcgccgggac
     3541 ctgcgatctc
//
LOCUS       DAOPOA010000154         3529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_28798_length_3529_cov_3.086644, whole genome shotgun sequence.
ACCESSION   DAOPOA010000154 DAOPOA010000000
VERSION     DAOPOA010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28798_length_3529_cov_3.086644"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PKJ17_04320"
     CDS             complement(<1..307)
                     /locus_tag="PKJ17_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOD75227.1"
                     /translation="MFQALIDNIPSPVYYKDARGVYLICNRAFREYFSVTDEQFIGKD
                     VFQLPLRRDEAEVHHAMDQQLLAEPGVRSYELRSVRQDGTVRYDLNKKATLTADDGSV
                     "
     gene            complement(468..1385)
                     /locus_tag="PKJ17_04325"
     CDS             complement(468..1385)
                     /locus_tag="PKJ17_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOD75228.1"
                     /translation="MKCAAIDIGTNTILLVVAERDDENEAFRDVVDMSTIVRLGEGLA
                     NSGRLSGPAMSRTMDGLRRYTDIAASHGVERLFCVGTAALREAANSGEFLASVKDAFG
                     FDVEVISARDEARYTYLSVRDDHTVRAPDMTIIDIGGGSTEVISGTDRDFGGYVSLPM
                     GSVRLTDAFVSHDPPIGEEMDRVVAYIREHLPAVTCRDGSTLVGTAGTVTNIAAMVAG
                     IRTYDKEIVHGFVIGLGQLKALIASLTGLTSDERRRVTGMEPGREDIIVQGAVILREI
                     MEHGGFDRVKVSAAGVRYGVIYERCGFAT"
     gene            1423..2163
                     /locus_tag="PKJ17_04330"
     CDS             1423..2163
                     /locus_tag="PKJ17_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOD75229.1"
                     /translation="MESGVLSPINVSGLAAILSGFLSFFTPCILPLVPSYLIYISGIT
                     VGDVAAPTKAHRRRVFFHSLSFVLGFSFVFVALGVSTSLLGGFFAEHQTLIIRLGGII
                     LILLGLFYLDIIKISFFNRQYMMQLEQKPVGLAGSFVVGLTFSLGWTPCVGPALSSIL
                     IVASMAGKTSHGTYLLSLYSLGLAIPFVISSLLFDKLFILLKRFGFIARYAVKVLGVL
                     LILLGLLMVSSYYNTLNLWLGTLLPPGA"
     gene            complement(2191..3090)
                     /locus_tag="PKJ17_04335"
     CDS             complement(2191..3090)
                     /locus_tag="PKJ17_04335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HOD75230.1"
                     /translation="MDDENILEKQPARERVEDSSLPTTFDPLRKYLSEVSRYSVLTRE
                     EEFEVARRVYEDKDRDAAQRLVMSNLKLVVKISLEYYNTYLNVLDLIQEGNVGLLHAV
                     KKFNPYKGTKFSTYASFWIRAYILKYIMDSWSLVKVGTTQSQRKLFYRLNKEKQKLEA
                     LGMFPAPALLANNLDVKEEEIEEMQKRLAFTDVSLDSPIHDESDDTIMDMIKGGDDVE
                     EVVSGKEKREILAQRVKEFKKDLNPKEVFIFEQRIMAEEPLTLQEIGQQFKISRERAR
                     QIENRVIKKFRDRFQNEFQELDL"
     gene            complement(3104..>3529)
                     /locus_tag="PKJ17_04340"
     CDS             complement(3104..>3529)
                     /locus_tag="PKJ17_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOD75231.1"
                     /translation="VSQYIARMANMVDFNVVVIDDREEYANTERFPDAGEIVVRDFVE
                     AFDSLNFTGHEFVVIVTRGHSHDADVLRAALSRNTRYVGMIGSRRKVQMIFDLMRQSG
                     YSDEVISRVYAPIGLSIHAETPQEIAVSIVSQLIQVRAE"
BASE COUNT          674 a         1105 c          896 g          854 t
ORIGIN      
        1 ccacacttcc gtcatcggcc gtgagtgtcg ccttcttgtt gaggtcataa cgcaccgtgc
       61 catcctgccg aacagatctc aattcgtagg acctgacccc cggctcggcg aggagctgct
      121 ggtccatcgc gtgatggacc tcagcctcgt cccgcctcag gggcagctgg aagacatcct
      181 tgccgatgaa ctgttcgtcg gtgacgctga agtattcgcg aaaggccctg ttgcaaataa
      241 ggtagacccc cctcgcatcc ttgtaataga caggactggg tatgttgtcg ataagcgcct
      301 gaaacatgtg aggcgttttg gaaaggatct ctacgagcgc ttccagtgta aactctcaga
      361 ttcaaaagaa tctttcaacg gcattatatg gttcttatca cagaaggacc acagcggtcc
      421 atagaaaaaa atgggtgtcc ggaggcctgc gggcggaagg cagccgacta cgtggcaaac
      481 ccgcaccttt cgtatatgac gccatacctg actcctgcgg cactgacctt gaccctgtcg
      541 aacccgccgt gttccatgat ctccctcaga ataaccgccc cctggacaat tatatcctct
      601 ctgcccggct ccatgcccgt gacgcgcctt ctctcgtcgc tcgtcaatcc cgtcaacgat
      661 gctatcagtg ccttcaactg cccgaggccg atgacaaagc catgcacgat ctccttgtcg
      721 tacgtcctga tcccggccac catggcggca atgttcgtca ccgtgcctgc cgtcccgaca
      781 agggtgctcc cgtcgcgaca ggtcaccgcg ggaagatgtt cccgtatgta cgcgacgaca
      841 cgatccattt cttccccgat gggagggtcg tgggagacga aggcgtcggt gagtctgacg
      901 cttcccatcg gcagcgagac gtatcctcca aaatcccggt cggtcccgct gatcacctcc
      961 gtgcttcctc ccccgatatc gattatggtc atgtccggtg cccgaacggt atggtcgtca
     1021 cgcacggata gataggtgta gcgcgcctcg tcccgggcgc ttatgacctc gacatcgaaa
     1081 ccgaaggcgt ccttcacgga ggcgaggaac tcgccgctgt tcgcggcctc cctcagcgcg
     1141 gcggtgccca cgcagaaaag ccgttcaaca ccatgtgacg cagcgatatc cgtataacgt
     1201 ctgagtccgt ccatcgtccg ggacatcgcg ggaccgctca ggcgaccgga gttggccagc
     1261 ccctccccga gcctcacgat ggtcgacata tcgacaacat cgcgaaacgc ttcgttctca
     1321 tcgtcccttt ccgccacgac gaggaggatc gtgttcgtac cgatatcaat tgcggcacat
     1381 ttcatttgac atcctcacga gaaaacccat aagatacgac tcatggaatc cggcgtcctg
     1441 tcaccgatca acgtgagcgg gctcgccgcc atcctgtccg ggttcctgtc cttcttcacg
     1501 ccctgcatcc tcccccttgt cccatcgtac ctcatctata tcagcgggat aacggtcggt
     1561 gatgtcgccg cccccacgaa ggcgcacagg agacgggtat tcttccattc cctgagtttc
     1621 gtcctcggat tctctttcgt cttcgtcgcc ctgggcgtct ccacgtcatt gctgggtggc
     1681 ttctttgccg agcatcagac gctcatcata cgcctcgggg ggatcatcct cattctcctc
     1741 ggcctcttct acctcgacat tataaagata tccttcttca accgtcagta catgatgcag
     1801 ctggaacaaa agcccgtcgg actcgcaggc tccttcgtcg taggcctgac cttttccctt
     1861 ggctggacac cctgcgtcgg cccggcgctc tcttcgatcc tcatcgtcgc gtccatggcc
     1921 gggaagacct ctcatgggac ctatctgctt tcgctgtatt cgctcggact ggcgataccc
     1981 tttgtcatct cctcactcct gttcgacaaa ctgttcattc tgctgaagag attcggtttc
     2041 atcgcgcggt acgccgtgaa ggtgctcggc gtgcttctca tcctcctcgg ccttctcatg
     2101 gtcagctctt actacaacac tctgaatctc tggttaggga cattgcttcc gccgggggcg
     2161 tgaccctcgg ggtcggggat gacggagggg ctagaggtcg agctcctgaa actcattctg
     2221 gaaacggtcc ctgaacttct ttatgaccct gttctcgatc tgccgcgccc tttcgcgtga
     2281 gatcttgaac tgctggccga tctcctgcag ggtcagcggc tcttccgcca tgatcctctg
     2341 ttcgaagata aagacctcct tcgggttcag gtccttcttg aattccttca ccctctgtgc
     2401 cagtatctcc cgtttttcct tgcccgagac cacctcttcg acatcgtccc ctcccttgat
     2461 catatccatg atggtatcgt cactctcatc gtggatgggc gaatcgaggg agacgtcggt
     2521 aaaggccagt ctcttctgca tctcctctat ctcttcttcc ttcacatcga ggttgttcgc
     2581 gaggagcgcc ggggccggaa acattccgag ggcctccagc ttctgctttt ccttgttcag
     2641 acggtagaag agcttcctct ggctctgcgt tgtccccacc ttgacgaggc tccaggaatc
     2701 catgatgtac ttcaatatgt aggcccgtat ccagaacgac gcgtacgtgg agaacttggt
     2761 tcccttgtag ggattgaact ttttcacggc gtgaaggaga ccgacattcc cctcctggat
     2821 gagatcgagg acattgagat atgtgttgta atattccagc gagatcttca ccaccagctt
     2881 gaggttcgac atgacgagtc tctgggcggc atccctgtcc ttgtcctcgt acaccctcct
     2941 ggcaacctcg aactcctcct cgcgtgtcag gacgctatac cgcgaaacct cggagagata
     3001 cttcctcagg gggtcgaagg tggtggggag tgaactgtct tcaacgcgct ccctcgccgg
     3061 ctgtttctcg aggatattct cgtcatccat tctaaacaaa cctctattcg gcccggacct
     3121 gtatcaactg actgacgatg ctcaccgcta tctcctgggg cgtttcggca tgtatggaaa
     3181 gaccgatggg ggcgtacacc cgggaaatga cctcgtccga atagcctgat tgccgcatga
     3241 ggtcgaagat catctgtacc tttctcctgc tcccgatcat gccgacgtac ctggtgttcc
     3301 ttgagagggc cgcccgcagc acatccgcgt catgagaatg accccgggtg acgataacga
     3361 caaattcgtg ccctgtgaag ttcagtgaat cgaaggcttc gacaaagtcc cgcacaacta
     3421 tctctccggc gtcggggaac cgctcggtat tggcgtattc ctccctgtcg tcgatgacga
     3481 cgacgttgaa gtcaaccatg ttcgccatgc gcgcgatgta ttgcgacac
//
LOCUS       DAOPOA010000155         3525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_28843_length_3525_cov_3.263401, whole genome shotgun sequence.
ACCESSION   DAOPOA010000155 DAOPOA010000000
VERSION     DAOPOA010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28843_length_3525_cov_3.263401"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKJ17_04345"
     CDS             complement(<1..562)
                     /locus_tag="PKJ17_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOD75232.1"
                     /translation="MGPLMLSVDAVTFLEEEGFPVLRSLLAASEDEAAVKAAEIGFPV
                     TLKLSSPDVVHKTEAGGIRVSLRDAAEVRQAFTEMVSAFTSGNPGKRLDGVMVQEQGW
                     GAEVIVGMLTDPQFGPVLMCGLGGVFVEVMKDVSFRLVPIERRDAREMLEELKGYGAL
                     RNPRKGPIDLAEVEDFLLGVSAFLRDH"
     gene            complement(550..1962)
                     /locus_tag="PKJ17_04350"
     CDS             complement(550..1962)
                     /locus_tag="PKJ17_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOD75233.1"
                     /translation="MKEKLARLFSPGSVAVVGASNSFDKLGYHVMKSLVGNYRGRIFP
                     VNPKGEKVWGLLSYPSLEAIPGDVDLAVVAVPAGMVGETLHACGRKGTSGVVLITAGF
                     KEIEDPVGASLEAEIGGVAGQYGLPIIGPNTFGFVNLSCDVNASFTSEFSRIQKGGVA
                     LVSQSGGICHLCGFLAIEQRVAMSKFMSLGNRCNVDFPELLEYLVEEDDSTKVIALYI
                     EGLDEPRRLLDVAKSLKGRKPIIALKAGKSEKGDSASRFHTGSLAGNQAIWRGALRQA
                     GILEVATAEELLDTAKAIDSCPLPPGARVAVLSGQAGPGMIASDALEPHGLSLSRFSE
                     ETQRRINDLLPPIAIRTNPVDMGPAWYNPGNIIEILRAVLEDEGTDGVIFLAMYASAN
                     LKLAEGMVEYMKAVDPFRKPVIACFTAPPGIWDGPIDALDRKKGIAILPTPERAAQAM
                     ANLWKVSALMNAEGVGTWDR"
     gene            complement(2207..3073)
                     /locus_tag="PKJ17_04355"
     CDS             complement(2207..3073)
                     /locus_tag="PKJ17_04355"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOD75234.1"
                     /translation="MKIGIIGAGKVGISLGYVLRGNGADVTVISDRLPSALETARGFL
                     GTDMAYTGDVMEVVRSCSVVAIATQDREIAGIARDIFKAAGDLTGKLFFHTSGADPSS
                     ILSPLDEKGAHLGSLHPLQTFPDIESAIEVLPDTSIFIEGDEKALAVLRVIAGHLGAS
                     IHTIAGKDKVFYHLAAVFVCNLLSALMHSGTGVMDRIDVSLEPFLPIIRATMKNIEAK
                     GPLAALTGPVVRGDDKTIRSHLAAMEDMPLHRDIYLALSKMALEMVRERKALSEAEIE
                     ILRRVLEGGEKT"
     gene            complement(3070..>3525)
                     /locus_tag="PKJ17_04360"
     CDS             complement(3070..>3525)
                     /locus_tag="PKJ17_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HOD75235.1"
                     /translation="RWKLLSKIFALLNVYDLVRGKIDFGKDGLVYTKMGASFTINKGI
                     YHTNNFLLDSPSMVITGAGDIDISKETINATLEVSPLVALDRTIDKIPIVRSILKNKN
                     KGFLYVTYSVSGSFDDPDISTNYVGTVGTKSLEILRNILVFPKEVFERK"
BASE COUNT          683 a         1110 c         1003 g          729 t
ORIGIN      
        1 gatggtctct gagaaaagcg gagaccccga gcaggaagtc ctcgacctcc gcaaggtcga
       61 tggggccttt tcggggattc ctgagcgctc cgtacccctt cagctcttcc agcatctccc
      121 ttgcgtcccg gcgctctatc ggaacgagcc tgaaggacac gtccttcatt acttccacga
      181 agacgcctcc gagtccgcac atgagaacgg gaccgaactg cgggtcggtg agcatgccca
      241 cgatcacctc ggcaccccat ccctgctcct gcaccataac cccgtcgagt ctctttccgg
      301 ggttgcccga ggtgaaagcg ctcaccatct ccgtgaaagc ctgtctcacc tcggcggcgt
      361 ccctgaggga gacccgtatg ccgccggcct ccgtcttgtg caccacgtcc ggagatgaga
      421 gcttgagagt aacaggaaag cctatctccg ccgccttcac agcggcttcg tcctcgcttg
      481 ccgccagaag gctcctgagg acagggaacc cctcctcctc aaggaatgtc acggcatcca
      541 cggagagcat caacggtccc atgtcccaac cccctcagcg ttcataagcg cgctcacctt
      601 ccacaggttg gccatggcct gagcggcccg ctccggcgtg ggaaggatcg cgatcccctt
      661 cttcctgtcc agcgcatcta tcggtccgtc ccatatgccg ggaggagccg taaaacaggc
      721 aatgacgggc ttccggaaag ggtcgaccgc cttcatgtac tcgaccatcc cttccgccag
      781 cttcaggttt gccgacgcgt acatggcgag gaaaatgacg ccgtccgtgc cttcgtcttc
      841 gaggacggct ctcagtatct cgatgatatt cccgggattg taccacgccg gccccatatc
      901 aaccggattg gtgcggatgg cgatgggcgg caggaggtca ttgatccgcc gctgggtctc
      961 ctccgagaag cgcgacaggc tgagcccgtg gggctcgagg gcgtcggagg cgatcatccc
     1021 gggacccgcc tggccggaga gaacggcaac gcgtgctccc ggaggcagag gacacgagtc
     1081 gatggccttt gccgtatcga gaagctcctc cgccgtggca acctccagaa tgcccgcctg
     1141 gcgcagggcg cctctccaga tcgcctggtt gccggcgagc gaaccggtat ggaaccggct
     1201 cgcgctgtcg cccttttcgc tcttgcccgc cttcagggca atgatgggct tccttccctt
     1261 aagcgacttc gcgacatcga gaagccttct cggctcatca agcccttcaa tatacagcgc
     1321 gatcaccttc gttgagtcgt cctcttccac aaggtattcg agaagctccg ggaaatccac
     1381 gttgcagcgg ttgccgaggc tcatgaactt cgacatggcc accctctgct cgatggcgag
     1441 aaagccgcag aggtgacaga ttcccccgct ctggctgacg agtgcgacgc cgcctttctg
     1501 tatccgcgag aattccgacg tgaatgaggc gttcacgtcg cagctcaggt tgacgaagcc
     1561 gaaggtgttg ggcccgatga tcggcagacc atactgcccg gcaacacccc ctatctctgc
     1621 ctcgagggag gccccgaccg ggtcttcgat ctccttgaag cccgcggtga tgagcaccac
     1681 gccgcttgtc cccttgcgcc cgcacgcgtg cagggtctca cccaccatac ccgcaggcac
     1741 ggcgacgacc gcaaggtcca cgtcgccggg tatcgcctcc agggaaggat acgacaagag
     1801 accccagacc ttctcgcctt tgggattcac cgggaatatc ctcccccggt aattgccgac
     1861 gaggcttttc atcacgtgat aaccgagctt gtcgaaactg ttggatgccc cgacgaccgc
     1921 aacggagccg ggagaaaaaa gccgggcaag cttctccttc atcacaaacc ccccgtgtca
     1981 acacattgaa tgaattccct tttactctgt agcacgacac caaagaaaaa acaacccacg
     2041 aagacctgtc gtgtcatctc ccgccgggac ggcgggacga gatcgccgcc ggatcctggg
     2101 ggctccgaga tccggcggcg atcttttctc tcttttctcc tctctttgcg acctctggga
     2161 gcttgagaga ggagcgaacg cgacgaacgg gcgtgagata aagtagttaa gtcttttccc
     2221 cgccttcaag cacgcgccgg aggatttcga tttcggcttc gctcaaggcc ttcctctccc
     2281 tcaccatctc gagggccatc ttcgagagcg ccagatagat atccctgtgg agcggcatgt
     2341 cctccatggc ggcgaggtgg gagcggatgg tcttgtcgtc tccacgcacg acgggacccg
     2401 tgagtgcagc gagggggccc ttcgcctcta tgttcttcat cgttgcccgt atgatgggga
     2461 ggaagggttc gagggacacg tctatccggt ccatcacacc tgttccggag tgcatgaggg
     2521 ccgaaaggag attgcagacg aagacggccg cgagatggta gaagaccttg tcctttcccg
     2581 cgatggtgtg aatactggcc cccaggtgcc cggcgatgac ccggagcacg gcgagggcct
     2641 tctcgtcgcc ttcgatgaat atgctcgtgt cgggaaggac ctcaatggcg ctttcgatat
     2701 cgggaaaggt ctgcaggggg tggagcgatc ccaggtgggc acctttttca tcgagaggag
     2761 agaggatcga cgagggatcg gcgccgctcg tatgaaagaa gagctttccc gtcaggtcgc
     2821 cggcggcttt gaatatgtcc ctcgcaatgc cggctatctc cctgtcctgc gtggcgatgg
     2881 ccacgacgct gcaactcctc acaacctcca tcacgtcccc cgtgtaggcc atgtcggtcc
     2941 ccaggaaacc acgcgccgtc tccagagccg agggaagcct gtcggagatg actgtaacgt
     3001 cggccccgtt gcccctgaga acgtaaccca gggagatgcc cacttttccc gctcctatga
     3061 ttccgatctt cattttctct caaagacctc tttcggaaaa acgagtatgt tcctcaggat
     3121 ctccagcgat ttcgtgccca ctgttcccac gtagttcgtg ctgatatcgg gatcgtcaaa
     3181 tgaccctgag acgctgtatg tgacgtagag aaaacccttg ttcttgttct tgaggatgct
     3241 cctcacgatg gggatcttgt cgatcgtcct gtcgagcgca acgagcgggg agacctcaag
     3301 cgttgcattg atcgtttcct tgctgatgtc gatgtcgccc gcccccgtta tgaccatcga
     3361 ggggctgtcg aggagaaagt tgttggtatg gtagatcccc ttgttgatcg tgaaggatgc
     3421 acccatcttg gtgtacacca gaccgtcctt gccgaaatcg atcttgcccc tcaccaggtc
     3481 gtacacgttg agaagggcga atatcttgga gaggagcttc catct
//
LOCUS       DAOPOA010000156         3497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_29163_length_3497_cov_3.217606, whole genome shotgun sequence.
ACCESSION   DAOPOA010000156 DAOPOA010000000
VERSION     DAOPOA010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29163_length_3497_cov_3.217606"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKJ17_04365"
     CDS             complement(<1..638)
                     /locus_tag="PKJ17_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75236.1"
                     /translation="MSVLRSIPFASDIIEEIAGIIGNESSTGEGTVIVFPGKRPSLYL
                     KARLATLAGPRAFFPPRCFSLDQFIDEIARRQNPGYADIDHGDAAFILFGLIRSLGAF
                     ERHALAGKGFGDFLHWGRHLLSFIDRLDMEGIENSALVNVERNASIGYDVPQSINELL
                     SNISLIRSEFHRVLGEGRWFTRGTKRIAAVEETGVRVPDDLRRVVFAGLFGLT"
     gene            complement(635..>3497)
                     /locus_tag="PKJ17_04370"
     CDS             complement(635..>3497)
                     /locus_tag="PKJ17_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOD75237.1"
                     /translation="LKASAMQLNLPPDFEVSTETKELIDLALRECLQRTGEDGRARAI
                     FDAFLESYIELEGDRAGWVPRDLLGAAISSLWNEETKENRQFVLPAGGAHAGAAALRA
                     SVADSASRLLTALADDTGLKPLSHLLAALKRCADPRENALSKSVYFERSLEDCLKKGS
                     DPPGPEERELWDTVVRLRQLYAGALAVSKYAPYLQVYSFFKEVFQREITYYRRVIPIE
                     ELNRLLQDIVSRRDFVPEIYYTLSERYIHFLLDEFQDTSLLQWKNVEVLAEEALSRGG
                     SLFLVGDRKQAIYRWRGGRPELVDDIAERYQDLYRVEELGLDTNYRSSEHIVAFNNAV
                     FDAENLAVLAGSVLGGHPPEAAREAVKPYRTSRQHFLEGKKGMGLVSIERLTAEGDDE
                     NGQDVFTGDEARQLVEERLKTLVQGIMARGVFRQGDLAVLVRTREEAGAVAAVLLSMG
                     LSVESEHTVSIRNNPLIREVICFLKFLDKPDDDLSFASFITGRIFAAKNGDWAARMPA
                     WLSERYLTDRDRFLYLQFKSDHGAVFEEGFAAFLSRANYLPLYDLVVNFYRHWEVLVR
                     FPDDVPYLLHFLELVKERERSDMSSLAGFIDFLEESPRGVSYGASNDDKAFLLATPDS
                     LNAVKVLTIHKAKGLQFPVVILPFVTLTSFSASSGRDKQRCFQCDDDGLRLFHLKKEY
                     TDVSPELAALYRTKEREYLSDELNNLYVAFTRPEEELYVLLTDKKRQRNHLIDNIFGM
                     EAFRPFANDRSITLGEPRKAARPGKDAATPRPGPAASFDDFTGEPAWAVKLQGSIADP
                     GQLSRRAVRARRKGDAIHRALSFIASLPVDDETIASLARAAAIHEGIPDAAREVERSL
                     KAFFMNRRFRAFFETGPGRSFYNERAIVDARGSTFKMDRVIVDPGLVQVIDYKTGEIR
                     SDTHVEQVSRYGRLLSEVYPDREVRGFLLYLDDGEVVSI"
BASE COUNT          658 a         1047 c         1125 g          667 t
ORIGIN      
        1 gtgaggccga agagcccggc gaagacgacg cgccgaaggt cgtcagggac acgcacacct
       61 gtctcctcga ccgcggcgat gcgcttcgtg cccctcgtga accaccggcc ctcgcccaga
      121 acccggtgaa actcgctcct gatcagagaa atattggaca gaagctcgtt gatgctttga
      181 ggcacatcgt agccgatgga cgcgttcctc tccacgttga cgagggcgct gttctcgatc
      241 ccttccatat cgaggcggtc aatgaaactg agaaggtgtc taccccagtg cagaaagtct
      301 ccgaacccct tgccggcgag agcatgccgc tcgaaggccc cgagggaccg tatgaggccg
      361 aagaggataa aggccgcgtc gccatgatcg atatcggcgt atccggggtt ttgcctccgg
      421 gctatctcgt cgatgaactg gtccagggag aagcaccggg gagggaagaa ggccctgggg
      481 cccgcgaggg ttgccagccg cgccttgaga tacagggaag ggcgcttccc cggaaagacg
      541 atgaccgtcc cttcgccggt tgaggactcg ttcccgatga tgccggcgat ctcctcgatg
      601 atgtccgagg cgaagggtat ggagcggagg acactcatat gctgacgacc tcgccgtcat
      661 cgaggtagag gaggaagcct ctaacttccc ggtcgggata tacctcggag aggagtctgc
      721 cgtaccggct cacctgttca acgtgggtgt cggagcggat ctcgccggtc ttgtaatcga
      781 tgacctgcac caggccggga tccactatca cccggtccat cttgaaggtg ctgcccctgg
      841 cgtccactat agccctttcg ttgtaaaaag aacggccggg gcctgtctcg aagaaggccc
      901 ggaagcgccg gttcatgaag aaggccttca gtgatcgctc gacctcgcgg gccgcgtcgg
      961 gtataccttc atggatggcg gcagccctgg caagggacgc gatggtctca tcgtcaacgg
     1021 gcagggacgc gatgaaggag agcgcccggt gtatggcgtc acccttcctg cgcgccctga
     1081 ccgcgcgtct cgagagttgc cccgggtcgg ctatactccc ttgaagcttc acggcccacg
     1141 caggctcgcc tgtgaaatcg tcgaaagagg ccgccgggcc gggccggggt gtggcggcat
     1201 ccttgcccgg tcgggcagcc ttcctcggct ctcccagggt tatcgacctg tcgttcgcga
     1261 aaggccggaa agcctccatc ccgaagatat tgtcgatgag gtggttcctc tgcctcttct
     1321 tgtccgtgag aaggacgtac agctcctcct cgggccgggt aaaggcgaca tagaggttgt
     1381 tgagctcgtc ggagagatat tcccgttcct ttgtccggta gagggcggca agctcggggg
     1441 acacatcggt gtactccttc ttcaggtgga agagcctcaa cccatcgtcg tcgcactgga
     1501 agcacctctg cttgtcccgg cccgatgacg cggagaagga cgtcagggtc acgaagggga
     1561 ggatgaccac gggaaactgg agccctttcg ccttgtgtat cgtgagcacc ttcacggcat
     1621 tgagcgagtc cggggtggcg aggagaaagg ccttgtcgtc gttggaggca ccgtatgaaa
     1681 ctccgcgggg gctttcctcc aggaaatcga tgaagccggc aaggctgctc atatcggagc
     1741 gctccctttc cttgacgagt tccaggaaat gcaggagata gggcacatcg tcggggaatc
     1801 ggacgagcac ctcccaatgc cggtagaaat tgacaacaag gtcgtaaagg ggaagataat
     1861 tggcccggga aaggaaagcg gcgaaccctt cctcgaagac agccccgtga tcggacttga
     1921 actggaggta caggaagcgg tcccggtccg taagatatct ctccgacagc cacgcgggca
     1981 tccgggccgc ccagtcgccg ttcttcgccg cgaatatccg tcccgttatg aatgacgcga
     2041 aggaaaggtc gtcatcgggt ttgtcgagga acttgagaaa gcagatgacc tcccggatga
     2101 gagggttgtt cctgatgctg acggtgtgtt ccgactcgac gctgagcccc atggacagga
     2161 ggaccgccgc gacggccccg gcctcctccc gcgtcctcac cagaacggcg aggtcgccct
     2221 gtcgaaagac gccccgcgcc attatcccct gaacgagcgt tttaagcctc tcctccacga
     2281 gctgccgggc ctcatcgccg gtgaagacgt cctggccatt ctcgtcatct ccctccgcgg
     2341 tgagccgttc gatggacacg agccccatgc ccttcttccc ctccaggaag tgctgacgcg
     2401 atgtccggta gggtttcacc gcctcccggg cggcctcggg ggggtgtccg ccgaggaccg
     2461 agcccgcgag gacggcaagg ttctcggcgt cgaagaccgc gttgttgaac gcgacgatgt
     2521 gctccgaact gcggtagttg gtgtcgagac cgagttcctc cacgcggtag aggtcctggt
     2581 aacgctcggc aatgtcgtcg acaagctccg gtcgccctcc gcgccagcga tatatcgcct
     2641 gtttcctgtc gcccacgagg aaaaggcttc cgccccggct caaggcctcc tccgcgagga
     2701 cctcgacgtt cttccattgc aggaggctcg tgtcctggaa ttcgtcgaga aggaaatgga
     2761 tgtatcgctc cgaaagggtg tagtagatct cgggaacgaa atccctgcgg ctgacaatgt
     2821 cctgaagcag gcggttgagc tcctcgatgg ggatgacgcg cctgtagtat gtgatctccc
     2881 gctggaatac ctccttgaag aaactgtaga cctgaaggta gggggcgtat ttcgaaaccg
     2941 ccagggcccc ggcataaagc tggcgcaggc ggaccacggt gtcccagagt tccctttcct
     3001 ccgggccggg cggatcgctg ccctttttca ggcaatcttc cagggagcgc tcaaagtaaa
     3061 cgcttttcga aagggcgttc tcccgcggat cggcgcacct cttaagcgcc gcaagaaggt
     3121 gggagagggg ttttaggccg gtgtcgtccg ccagggccgt aaggaggcgc gacgcgctgt
     3181 cggcgacgct ggcgcgcagg gcggcggcgc cggcatgggc gccgccggcg ggcaggacga
     3241 attgccggtt ctccttggtc tcttcattcc agagggagga gatggcggcc ccgaggaggt
     3301 cccgggggac ccagcccgcg cggtcacctt cgagttcgat gtagctctcg aggaacgcgt
     3361 cgaagattgc ccgggctcgt ccatcctccc ctgtcctctg cagacattcc ctgagggcga
     3421 ggtcgatgag ttcctttgtt tccgtcgaca cctcgaagtc cggcggcagg ttgagctgca
     3481 tcgcggaggc cttgagc
//
LOCUS       DAOPOA010000157         3486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_29304_length_3486_cov_2.832119, whole genome shotgun sequence.
ACCESSION   DAOPOA010000157 DAOPOA010000000
VERSION     DAOPOA010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29304_length_3486_cov_2.832119"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PKJ17_04375"
     CDS             <1..1092
                     /locus_tag="PKJ17_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HOD75238.1"
                     /translation="DLSYIMYTGGTTGFPKGVPGNHTGEVTYINDIMEDVIKDFVDPG
                     NDTVLMINPLFHIMAKGFSIAFGFNFANTVVLMPVPEVDPTLSAIERYRIKWMLGVPA
                     LYRMMLESDRIDQYDLSSLRYCYCGGDVLPVEVFKTWQEKYSIPLYQVYGSTEIGHVT
                     YSLLTQEPSPTVIGTPLKTRKSIVVDRDTLEKLPPGELGELLVTSPYTLKEYVNKPEE
                     TAYSYVQLNGDIYYRMGDYVKMNERGELEFVERTADIIKYKAYRVSASEVEAALQDHP
                     TVIGACVIGIPDPKVGERIKAIVVLKEDAKGIGSTELLKWCRDRLAPYKVPGYIEFRD
                     MLPKSKVGKLLRREIRDEEKRKQEKEKRR"
     gene            1450..2697
                     /locus_tag="PKJ17_04380"
     CDS             1450..2697
                     /locus_tag="PKJ17_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOD75239.1"
                     /translation="MKRSHAAYGMMVLAVVVCLVFLASPGRDTRKPVFGDDAPKNLRK
                     VSGTVRKGDVLSRLFQRNNLHVADLSRVKEAADGVYDLGKLSSGRPYSMTIDTDDRLN
                     SLTYWIDDDTYLSLSRDGESLTAAIEKVEYDKRQLSFSGIIEDNLVASLGPGRDAVLL
                     ALDLSDIFAWDIDFNTDLRKGDTFRIVVEGLFSGNTFRKFGDILSAEFINDGQRYLAY
                     RYDVKGHAGYYDVEGRPLKRAFLKAPLNFRRISSYYSGRRLHPILKVYRPHKGIDYVA
                     AAGTPVSALGDGVVHFAGCRGGYGRLVIIRHRNGYSTWYGHLSRITKGIRSGSRVAQG
                     DIIGRVGSTGLATGPHLHFEMRVAGRAVNPLAIRSVRAEPLGAGHRTRFRKYVASMER
                     RLAEAMPLTGTSIPSMLASVERE"
     gene            2698..>3486
                     /locus_tag="PKJ17_04385"
     CDS             2698..>3486
                     /locus_tag="PKJ17_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75240.1"
                     /translation="MEERERSPAAERYICVHCHFYQPPRENPWLESVEVQDSAYPFHD
                     WNERITSECYAPNLASRIVNGYGRITDIVSNYSRISFNFGPTLLAWMRTFAPAVYEGI
                     LQADRLSVSLRSGHGNALAQVYNHIIMPLANARDKRTQVIWGIRDFQKRFGREPEGMW
                     LAETAVDLETLEHLATGGILFTILAPHQAMKVRKIGSGKWKDVTGGRIDPTRPYIVRL
                     PSGRRITVFFYDGPISNAIAFEGLLKRGEDLAMRLVSGFRSDRKW"
BASE COUNT          804 a          917 c         1027 g          738 t
ORIGIN      
        1 gatctttcct atatcatgta caccggaggc acgacgggat ttcccaaggg agttccgggc
       61 aatcacaccg gagaggtaac ctatataaac gacatcatgg aggacgttat aaaggatttt
      121 gtcgatccgg ggaacgatac cgttctgatg atcaatcctc ttttccacat catggcgaag
      181 ggtttttcca tcgctttcgg atttaatttt gccaacacgg tggtactcat gcccgtgccg
      241 gaggtggacc ccacgctatc cgcgatcgag aggtacagga tcaagtggat gctgggtgtg
      301 ccggcactct acaggatgat gctggagagc gacaggatcg accagtacga tctcagttcg
      361 ctgaggtact gctactgcgg tggagacgtt cttcccgtgg aggtcttcaa gacgtggcag
      421 gaaaaatact cgataccatt gtatcaggtc tacggttcaa cggagatagg gcatgtcacc
      481 tacagcctgc tcacgcagga gccttccccc accgtcattg gaacgcccct gaagaccagg
      541 aaatcaatag tcgtcgacag ggacacactg gagaaactac cgcctggtga gctgggagag
      601 ctgcttgtaa cgtctccgta caccttgaag gagtatgtca acaaaccgga ggaaacggcc
      661 tattcctacg tgcagctgaa cggtgacatc tattaccgta tgggcgatta cgtcaagatg
      721 aacgaaagag gagaactgga gttcgtcgag aggaccgccg acatcataaa atacaaggcg
      781 taccgggtat cggcctccga ggtggaggct gcccttcagg accatccgac agtcattggc
      841 gcatgcgtca tcggcattcc ggacccgaag gtcggagaga ggatcaaggc gatcgtcgtt
      901 ctgaaggagg acgcaaaggg gatcggaagc accgagcttt tgaagtggtg cagggaccgg
      961 ctcgctcctt acaaggtccc tggttacata gagttccggg atatgctgcc gaaatcgaag
     1021 gtcggcaagc ttctccggcg tgaaatacgc gatgaagaaa agcgcaagca ggagaaggaa
     1081 aagaggaggt agaaggaaag aagaccgttt caaccaaaac ccttagtttc aaagctccat
     1141 gaagtggttt taaggcttca aagattcaag aaagggacaa tttcacttgc tttgccatgc
     1201 aagggggacc taccccccct ccttgatggc aagagggggg caagggagat cgtcccttcc
     1261 tcttttctga tatttttcct tgcgatttca tcaatttggt tttaatcctt taattaaaac
     1321 cacaggaacg tgtaaaatag tttacagaac ttcaggtata ttttgaatag agtctaatac
     1381 aaaagactgt aaggaggtcg gtattctgaa gcgatacgaa aaaagcgccg gtccactgcg
     1441 gggcgggtca tgaaaaggag ccacgcggcc tatggaatga tggtcctggc ggtcgtcgtc
     1501 tgtctcgtgt tccttgcttc gccgggacgc gatacgcgca agcccgtctt tggggatgac
     1561 gcgcccaaga atctccgaaa ggtatcgggg acggttcgga agggagacgt cctctcgcga
     1621 ctcttccagc gcaacaacct ccatgttgcc gatctcagtc gcgtgaaaga ggccgccgac
     1681 ggtgtctatg acctcggtaa gctttcctcg ggacgtccat acagcatgac catagacacc
     1741 gacgacaggc tgaactcgct tacctactgg atcgatgatg acacgtacct cagtctttcg
     1801 cgggatgggg aatcgctgac agcggccatt gagaaggtgg agtacgataa gcggcagctc
     1861 tccttcagcg gtatcatcga ggataacctg gtggcctccc tgggccccgg cagggacgcg
     1921 gtgcttctcg ccctggatct gtcggacatc ttcgcttggg acatagactt caataccgac
     1981 ttgaggaaag gggacacgtt caggatagtc gtcgaaggac tcttttcggg gaataccttt
     2041 agaaagttcg gcgatatcct gtcggccgag ttcatcaacg acgggcagag atatctggcc
     2101 taccggtatg acgtaaaagg acacgcgggc tactatgacg tcgaggggag gcccctgaaa
     2161 cgggcttttc tcaaggcgcc cctcaatttc cggcgcatca gctcctatta ttccggcagg
     2221 cgcctgcacc ccatactcaa ggtttaccgg cctcacaagg gcattgacta tgtcgcggcg
     2281 gcagggaccc cggtctccgc cctgggtgac ggcgttgttc atttcgccgg ctgccgcggg
     2341 ggttacggaa gactcgttat catacggcac cggaacggtt acagcacctg gtatggtcac
     2401 ctgtcgcgca tcacaaaagg catcaggagc gggtcgagag tggcgcaggg tgacatcata
     2461 ggccgggtcg gttccaccgg ccttgccacg ggcccccatc tccacttcga gatgcgcgtg
     2521 gccggaaggg ccgttaatcc tctcgcgatc cgctccgttc gcgcggaacc ccttggcgcg
     2581 gggcacagaa ctcgtttcag aaagtacgta gcttctatgg aacggcgcct tgccgaagcg
     2641 atgccgttga cggggacatc gataccgtcg atgctggcgt cagtggagag ggaataaatg
     2701 gaggaacgtg agcgctcgcc cgccgcggaa cgatacattt gcgtccattg ccatttctat
     2761 cagccgccgc gggaaaatcc ctggcttgaa tccgtcgagg tgcaggactc ggcataccct
     2821 tttcacgact ggaacgagcg gatcacttcc gaatgttacg cgccgaatct tgcttcacga
     2881 attgtcaacg gctatggccg aatcaccgat atcgtcagca attattccag gataagcttc
     2941 aatttcgggc cgacactgct ggcgtggatg cgcaccttcg cgcctgccgt gtacgaaggg
     3001 atcctccagg cggacaggtt gagcgtttcc ctgcgttcag gccacgggaa cgccctggcc
     3061 caggtgtaca atcatatcat catgccgctg gcaaatgccc gtgacaagcg gacgcaggtg
     3121 atctggggga tcagggattt ccaaaaaagg ttcggacgcg agccggaggg aatgtggctt
     3181 gcggagaccg cggtggacct cgagacgctg gagcacctgg ctaccggcgg tatccttttc
     3241 accattctgg cgccacacca agccatgaag gtcagaaaga tcggctcggg gaagtggaag
     3301 gatgtgaccg gggggcgcat cgatccgacc cgaccctaca tcgtgcggct tccctcgggt
     3361 cggcggataa ccgttttctt ctacgacggg cccatatcga atgcgatcgc cttcgagggc
     3421 ctcctgaaaa ggggtgagga cctggctatg aggcttgtct cgggtttccg cagtgaccgg
     3481 aagtgg
//
LOCUS       DAOPOA010000158         3481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_29343_length_3481_cov_3.998541, whole genome shotgun sequence.
ACCESSION   DAOPOA010000158 DAOPOA010000000
VERSION     DAOPOA010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29343_length_3481_cov_3.998541"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..819)
                     /gene="queC"
                     /locus_tag="PKJ17_04390"
     CDS             complement(115..819)
                     /gene="queC"
                     /locus_tag="PKJ17_04390"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOD75241.1"
                     /translation="MKETTTRKAVVLLSGGVDSTTTLAIARNEGFEAYALTFRYGQRH
                     TCEIDAARETARYIGVARHVILDIDLRSFGGSALTDDIEVPKGRGLTDIAADIPVTYV
                     PARNTIFLSFALAFAETLGAQDIYIGVNALDYSGYPDCRPEYIAAFERMANLATKAGV
                     EGRSFRIHAPLIAMTKAEIIRTGVRLGVDYSLTTTCYDPTDDGKACGRCDACLLRLEG
                     FRQAGIEDPVPYACEG"
     gene            complement(829..1032)
                     /locus_tag="PKJ17_04395"
     CDS             complement(829..1032)
                     /locus_tag="PKJ17_04395"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOD75242.1"
                     /translation="MGKTLIILGVILIVIGLAFTYGPKIPWLGKLPGDISIKKDNFSF
                     YFPITTSIIISIILTILFTIFRK"
     gene            complement(1041..1586)
                     /locus_tag="PKJ17_04400"
     CDS             complement(1041..1586)
                     /locus_tag="PKJ17_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOD75243.1"
                     /translation="MDLEGKHIFLHICCAPCSIYTLKELRTEGARVSGYFYNPNIHPY
                     TEFSKRLSTLKEYAKIALLPLIVDGSYELEGFLAGALALGKDRCLSCYRMRLERAFQK
                     AAGGGFDALTTTLLYSRYQRHDDIKRIGEELCAAYGVPFLYRDFRTGWREGIEESKKL
                     GMYRQQYCGCIFSEKERYGGK"
     gene            1842..2213
                     /locus_tag="PKJ17_04405"
     CDS             1842..2213
                     /locus_tag="PKJ17_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75244.1"
                     /translation="MIKYILYVLRWTVLAIPGALFFNKVREFLGIENVYLAMIISQAL
                     MGAMVYFVDRLIFTSGAVPVPWEIKPRGVCVDCGHVGKCYRMPGERCCETGPRTEDSS
                     FRCEKCALKRLKETGTRDGSS"
     gene            2495..3295
                     /locus_tag="PKJ17_04410"
     CDS             2495..3295
                     /locus_tag="PKJ17_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75245.1"
                     /translation="MADHNSLKTTGILKYLPAVLVVAGQASVILSLRFEEAMPWLLAL
                     GMALAATGLGLRARSVGRRVVSWVIMGIVFPLLVPLVGFAFMVIKGKKGEAVSPSGAK
                     PVSIPAHAATAFILFLLAALFMDSLSLALISFVIAVIWVSTRKEDRYKGRVRLAVIGV
                     YALMVVMVFAMKGINYRVSQANARVIIEACERYKGRTGEYPAGLRDLVPAYLPEVPVA
                     RYTVMSGGFYYHRNLGPSGSDYRLVFVYESPFARSVYSSVDRGWRSID"
BASE COUNT          692 a          972 c         1057 g          760 t
ORIGIN      
        1 gccggaccag aggttgcagc ccgagaacct caggaacacc gccgggcggc ccgcgttgat
       61 gccctcaccc tgaagggaac ggaagatctc tttgatgagg tatgccatgg gatcttaacc
      121 ttcacatgca taggggacag ggtcctcgat gccggcctgc ctgaacccct cgagcctgag
      181 caggcaggcg tcgcagcgcc cgcaggcctt tccgtcgtcc gtcgggtcgt agcaggtggt
      241 cgtcagggag tagtcaacgc cgaggcgcac ccccgtgcgg atgatctcag cctttgtcat
      301 ggcgataagg ggcgcgtgga tccggaagga cctcccctcc accccggcct tcgtggcgag
      361 gttcgccatg cgctcgaagg ccgcgatgta ttcgggcctg cagtcgggat aaccgctgta
      421 atcgagggcg ttgacgccga tgtagatgtc ctgggccccg agcgtttcag cgaaggcgag
      481 cgcgaaggac aggaagatcg tgttccgcgc gggaacgtag gtgacgggga tatcagcggc
      541 gatgtccgtg aggcccctcc ccttggggac ctcgatgtcg tccgtcagtg ccgaaccgcc
      601 gaagctcctg aggtcgatgt cgaggatgac gtgccgggcg acgccgatgt accgcgccgt
      661 ttccctcgcg gcatcgatct cgcaggtgtg ccgctggccg taacggaagg tgagggcgta
      721 agcctcgaac ccctcgttcc tggcgatggc gagcgttgtc gtcgagtcca cgccgccgct
      781 taagagaacc accgcttttc ttgtcgtcgt ctccttcatg tcaggtccct actttcggaa
      841 tatggtaaag agtatcgtca ggatgatgct gattattatg ctcgtcgtga tgggaaagta
      901 aaagctgaaa ttgtccttct taatggagat gtcgccggga agctttccga gccagggtat
      961 tttcggcccg tacgtgaagg cgaggccgat gacgatgaga atgaccccaa ggataatgag
     1021 cgtttttccc atggatggca tcattttccc ccgtagcgct ccttttcgct gaatatgcat
     1081 ccgcagtatt gctggcggta catgccgagc ttcttcgatt cctcgatacc ttcccgccag
     1141 ccggtgcgga aatcgcggta gaggaagggg acgccatagg ccgcgcagag ttcttcgcct
     1201 atcctcttta tgtcgtcgtg ccgctggtac ctgctgtaaa gaagggtggt ggtgagcgcg
     1261 tcgaaaccgc cgcccgcggc tttctggaag gccctctcga gacgcatgcg gtagcaggag
     1321 aggcagcggt ccttgccaag ggcgagggct cccgcaagaa aaccctccaa ctcgtacgag
     1381 ccgtccacga taaggggtaa gagcgctatc tttgcgtact ccttcagtgt ggaaaggcgc
     1441 ttcgagaact ccgtataagg atggatgttg gggttgtaaa aataccccga gaccctggcc
     1501 ccttcagtgc gcagttcctt gagggtgtat atgctgcagg gcgcgcagca gatgtggaga
     1561 aatatgtgct ttccttcgag atccattggt cggtcttccc aattgcccgt aatttcttat
     1621 aatttaccac cttccgtccc tattttccac atcggcggag aaagatcgct gcctccgggg
     1681 gtccatcttc cgggccagga cagggaaagc gccttcctcc cccggcatcc cgggcgcagg
     1741 acccgaaacg gtcgttgccg ccggccacct cttcctgttg catatcggcc ctgcctgtct
     1801 cataataatg atgacatagt cgcttgcggc ggggaggcag tatgatcaag tatatcctct
     1861 acgtcctgcg atggaccgtt cttgccatcc cgggcgctct ttttttcaac aaggtcaggg
     1921 aattcctggg catcgaaaac gtttatctcg cgatgataat cagccaggcc ctgatggggg
     1981 cgatggtcta cttcgtggac aggctcatct tcacatcggg cgccgtaccg gtcccctggg
     2041 agataaagcc gcggggcgtc tgcgtggact gtggacacgt gggtaaatgc taccggatgc
     2101 cgggggaaag gtgttgcgag acgggcccga ggacggagga tagttctttc aggtgcgaaa
     2161 aatgtgccct gaagagactc aaagagacag ggacccggga cggaagcagc tgacccccgc
     2221 gtgccgtcat agccgggtct gacggacctt cacgccacag acggaacgat tcgctctccc
     2281 gttaccatcc ccttctggat ctatatgcat tatcgacatc gcgaggaagg atctcgcgga
     2341 caacaataag atgatatggc ttcttgttgt tgtcttgtta tatactctgg gggtggcgct
     2401 ttacgtcgcc atcggcagga agcaaagggt cggaaggacg ggttgagcac catccgtcgc
     2461 gagacaaggt cttcaaggaa acccgggtgt acccatggca gatcataatt ccctcaagac
     2521 cacaggcatt ctgaaatacc tccccgccgt cctcgtcgtg gcgggtcagg cttcggtcat
     2581 actttccctg cgattcgaag aggcaatgcc gtggcttctt gccctcggca tggccctcgc
     2641 cgcgacgggt ctgggactgc gtgcccgaag cgtgggccgc cgcgttgtct cctgggtcat
     2701 catggggatc gtcttccctc tccttgtccc cctcgtcggc tttgccttca tggtgatcaa
     2761 agggaaaaag ggagaggccg tcagcccttc cggggcaaag cccgtgagta tcccggccca
     2821 tgcggcgacg gcattcatcc tgttcctcct cgccgccctc ttcatggact ccctgagcct
     2881 tgcgctcatc tcttttgtca tagccgtcat atgggtctca acccgcaaag aggaccggta
     2941 taaaggcagg gtgcggctgg cggtgatcgg tgtgtacgct ttgatggtcg tgatggtctt
     3001 cgcgatgaag ggcatcaact atcgcgtatc ccaggccaac gcgagggtga tcatagaggc
     3061 gtgcgaaaga tacaagggca ggaccggcga gtacccggcc ggattgaggg acctcgtgcc
     3121 tgcctacctt ccggaggtcc ccgttgcgcg ctacaccgtc atgtccggcg ggttctatta
     3181 tcaccggaat ctcggcccgt cggggagcga ctacaggctg gtgttcgtct acgagtcccc
     3241 ttttgccagg agcgtttaca gttccgtgga caggggctgg cgcagcattg actagatgga
     3301 tattttgctg aattgacaga ttttcacaaa aaggcaggaa caatagcgac cgtaacggtg
     3361 tcgtagtgat atataagatt gcgatccact cagcacacaa acgaaaaagg aggtatgtta
     3421 tggattcgac gaacaggaga aaggatcaga tgagcagcat ggcaagaagg acggtgcctt
     3481 c
//
LOCUS       DAOPOA010000159         3470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_29460_length_3470_cov_3.020791, whole genome shotgun sequence.
ACCESSION   DAOPOA010000159 DAOPOA010000000
VERSION     DAOPOA010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29460_length_3470_cov_3.020791"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKJ17_04415"
     CDS             complement(<1..442)
                     /locus_tag="PKJ17_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75246.1"
                     /translation="MPFKRKSIGELSVVIKGAGEMASGIAHRLHRAGIRRVLMLDMPR
                     PLCVRRFVSFCEAIHDGVAEVEGVMGQLADDASRADAIWGADRIAVVVDPEWKSIPAL
                     SPDIVVDAVMAKRNLGTKRDEAPFVIGVGPGFTAPRDVHAVVESN"
     gene            complement(470..1084)
                     /locus_tag="PKJ17_04420"
     CDS             complement(470..1084)
                     /locus_tag="PKJ17_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOD75247.1"
                     /translation="MTGIYSVILAAGSSKRLGFNKLTLLMDSEPVIRRTAIPFVEAGL
                     GGEVIVVAGSDTVPVAAALEGLNVRVVRNEDHRQGMSSSIRAALPWIAEATAVFFHLG
                     DKPLVKKELLIAMVDRYRETRRGIIVPVHDGMKGHPVLMSHGPYREEMGSLDGDKGLR
                     EVIEKHSADVLFIEGDEGVLFDIDTGEDLEALRRRGYRVEKGAF"
     gene            complement(1100..1312)
                     /locus_tag="PKJ17_04425"
     CDS             complement(1100..1312)
                     /locus_tag="PKJ17_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOD75248.1"
                     /translation="MIDKRTSIREVLAKHPETARVFEEFGLGCAGCEAALFESVEQGA
                     QVHGVDVDSLIEGLRETMGEGWNSER"
     gene            complement(1358..1891)
                     /gene="amrA"
                     /locus_tag="PKJ17_04430"
     CDS             complement(1358..1891)
                     /gene="amrA"
                     /locus_tag="PKJ17_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOD75249.1"
                     /translation="MALSAEEKQKLKTLVKDAIEGVLFGREAIPVEVTESLKEKCGAF
                     VTVRNCGNLRGCIGYVRGYLPLHETVREMAIQAAFNDPRFDPVTRDEWKDVDFEISVL
                     TPMKRIEDVSEIEVGVHGLYIEKGVHSGLLLPQVATEQKWDRTEFLEYTCYKAGLPKD
                     AWKSKDADIFIFSADVF"
     gene            complement(1909..2739)
                     /gene="amrB"
                     /locus_tag="PKJ17_04435"
     CDS             complement(1909..2739)
                     /gene="amrB"
                     /locus_tag="PKJ17_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOD75250.1"
                     /translation="MTTVRLPSVSGMFYPEDPVLLRKDIEAYLKGAEVEPISGRVHAI
                     VSPHAGYVYSGQVAAFAYKAVSALEVDSVIVIAPSHRAYFEGIALWETGSFRTPLGDI
                     RIDEKGAGELLETRDVFTANRNVHKAEHSLEVQLPFLQCVFKDVLLLPLLMGAATEEL
                     YEKASDVLKEMILASPSRYLIVASTDLSHYYPYASAVKIDAVTVEHLRNFDIGAMVRD
                     TRSEKAQACGAGPMITAMMAARKLGAGTGRVLKYANSGDVSGDRSGVVGYVSAIFFEK
                     "
     gene            2835..3365
                     /locus_tag="PKJ17_04440"
     CDS             2835..3365
                     /locus_tag="PKJ17_04440"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HOD75251.1"
                     /translation="MTAKDILREISNHASCFIIPALLFLTCAFPDHTLGEPDKPVIGG
                     EEYVRILPYGFTVPARIDTGAATTSLDARNIRVEESTVTFALPPKWGGATIKARIVDW
                     KYVRTSKSREKRPVIELELCIASKQLRARVNLNDRSRMRYPLLIGRNVITGNFLVDTS
                     QSFWNSPETEDTEVDR"
BASE COUNT          710 a         1158 c          875 g          727 t
ORIGIN      
        1 ggttgctctc caccacggca tggacatccc ggggagcggt gaagcccggg cccacgccta
       61 tgacaaaggg agcttcgtcc cttttcgttc cgagattcct cttggccatg acggcgtcaa
      121 cgacgatatc gggagagagc gctggaatgc tcttccattc cgggtcaacg acaacggcga
      181 tccggtccgc cccccagatg gcatccgccc gggatgcatc atccgcgagc tgacccataa
      241 cgccctcgac ctcagcgaca ccatcatgga tggcctcgca aaaagagacg aaccgccgga
      301 cacacagggg cctcggcata tcgagcatga ggacacgccg tatcccggcc cggtgaagcc
      361 tgtgggcgat acccgaggcc atctcaccgg ccccctttat gaccacggac agctcgccaa
      421 tactctttcg cttgaacggc accgccttga acctcctctg tcggctgtct cagaacgcgc
      481 ctttttcgac cctgtatccc cgtcgtctca gggcctccag atcctctccc gtatcgatat
      541 cgaaaaggac gccttcatcg ccttctataa atagcacatc tgcgctgtgc ttttctataa
      601 cttcacggag gcctttgtct ccatcgagag atcccatctc ctccctgtag ggcccgtggc
      661 tcataaggac cgggtggccc ttcattccgt catggacggg aacgatgatg cccctgcgcg
      721 tttcacgata ccggtcgacc atggcgatca ggagttcctt cttcaccaaa ggcttatccc
      781 cgaggtgaaa gaagaccgcc gtcgcctccg ctatccacgg cagggccgcc ctgatggagc
      841 tcgacatccc ctgacggtgg tcttcattcc gcaccaccct cacattcaag ccttcgaggg
      901 ccgccgcgac agggacggta tcgctcccgg caacaactat gacctcaccg ccaagaccag
      961 cttccacgaa agggatcgcg gtcctgcgga tgactggctc ggaatccatg agcagggtca
     1021 gcttgttgaa gccgagcctc ttcgatgagc ccgcggcgag tatcacggag tagatgcctg
     1081 tcatgagata tgaattccgt caacgctcac tgttccagcc ttcccccatt gtctcgcgga
     1141 ggccctcgat gagactatcc acgtcaacac cgtggacctg cgctccctgc tcaacactct
     1201 cgaagagcgc ggcctcgcat ccggcacatc ccaggccgaa ctcctcgaat accctggcag
     1261 tctccggatg cttcgcgagg acctcccgaa tggacgtcct tttgtcgatc attccttccc
     1321 tccggccctg tctcggaaat cacgctggtt ccgctgttca aaaaacatcc gcggaaaaga
     1381 tgaagatatc ggcgtccttc gatttccacg cgtctttcgg aagacccgcc ttgtagcacg
     1441 tgtactccag gaactccgtc ctgtcccatt tctgttccgt cgccacctgg ggaagcaaga
     1501 gccccgaatg cacgcccttc tctatgtaaa ggccatggac gccgacctct atctcgctca
     1561 cgtcctctat cctcttcatc ggtgtcagga ccgagatctc aaaatcgacg tccttccact
     1621 cgtcgcgggt gacgggatcg aacctgggat cgttgaaggc cgcctggatc gccatctccc
     1681 tgacggtctc gtgaagggga agataacccc tcacgtaacc tatgcatccc ctcagattgc
     1741 cgcagttcct gacagttacg aaggcgccgc acttctcctt cagggattcc gtcacctcaa
     1801 cggggatcgc ttccctgcca aagagaacac cttcgatcgc gtccttgacg agcgtcttga
     1861 gtttctgctt ttcttctgct gaaagtgcca ttcatccatc cccggcagtc atttttcgaa
     1921 gaatatcgcc gacacatacc ccacgacacc gctgcggtct cccgacacgt cgcccgaatt
     1981 ggcgtacttc agtaccctgc cagtcccggc cccaagcttc cgggcagcca tcatggcggt
     2041 gatcatcggt cccgccccgc acgcctgcgc tttctcagac cgcgtgtccc tgaccatcgc
     2101 cccgatatcg aagttcctca gatgctccac cgtgacggcg tcgatcttca cggccgaggc
     2161 ataggggtaa taatgggaaa ggtccgtgct cgccacgatg agatagcgcg acggggaagc
     2221 caggatcatc tccttgagga catcggacgc cttctcgtac aactcttccg tcgccgcccc
     2281 catgagcaag gggaggagca gaacatcttt gaagacgcac tgcaggaaag gcagctggac
     2341 ctccagggag tgctccgctt tgtggacatt cctgttggcc gtaaagacat ccctcgtttc
     2401 aaggagttcc ccggcgcctt tctcgtcgat ccttatgtcc cccagcggcg ttctgaagct
     2461 gcccgtctcc cataaggcaa tgccttcaaa atacgccctg tggctcgggg cgatgacaat
     2521 gacactgtcg acctcaagag cggataccgc cttgtacgca aaggccgcca cctgccccga
     2581 atagacatac cccgcatgcg gagagactat cgcgtggacc cgaccggaga tgggctcaac
     2641 ctccgcccct ttcaggtagg cttcgatatc ctttctaaga aggacgggat cttcgggata
     2701 gaacatcccg ctcactgaag gtaagcgtac cgttgtcatc tttcgacccg taccagtcca
     2761 ttatatcata tgtgaccgcc gggagcgcgg cttttttgtc tatcccgcga aggctatgtg
     2821 ctataataca tgctgtgaca gccaaggaca tcctccggga aataagcaac cacgcctcct
     2881 gcttcatcat cccggcactg ttgttcctca cctgcgcctt tcccgaccat accctgggcg
     2941 agccggataa acccgtcata ggaggcgaag aatatgtcag gatactgccc tacgggttca
     3001 cggtcccggc gcgcatcgat acgggagccg caacaacatc cctggacgcc aggaacatac
     3061 gcgtggagga gtcgaccgtc accttcgctc ttcctcccaa atggggaggc gccaccatca
     3121 aggcccgtat cgtcgactgg aagtacgtcc gcacctccaa gtcgcgcgag aagcggccgg
     3181 tcatcgaact ggaactgtgc atcgcctcaa agcagctgcg ggcccgggtc aatctcaacg
     3241 accgctcgcg catgagatat cctttgctca tcggcagaaa cgtcatcaca ggcaatttcc
     3301 tcgttgatac ctcgcaatcc ttctggaatt ctccggaaac agaggataca gaggtcgaca
     3361 gatgaaacga ccggccctgg cggcagcgct ggcgctcatt gccatatctc ttatcattgt
     3421 cacctaccgc atcgtctcgc tgggttatcc gctcaccccc gcccggcccg
//
LOCUS       DAOPOA010000160         3467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_29489_length_3467_cov_4.566823, whole genome shotgun sequence.
ACCESSION   DAOPOA010000160 DAOPOA010000000
VERSION     DAOPOA010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29489_length_3467_cov_4.566823"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..2665)
                     /gene="infB"
                     /locus_tag="PKJ17_04445"
     CDS             complement(68..2665)
                     /gene="infB"
                     /locus_tag="PKJ17_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOD75252.1"
                     /translation="MTKIKITALTDKASDDEILAKLKKIGVKIKDKPKEGEAAHEAVK
                     EQTSATGETLVETRVASTIIRRRVQPPPKAKEEPIEEKPGAEEEKKAEAAGTKVPTRK
                     RAPKAPRLEEEIAEETPRKIPEDLHVHIEERQPEEAPPPEIPEAVILTPEDKAPKGDI
                     EHIAEEKEKEITKALEEAYKQDLEKDFTAVEGEGEEEGPERQKKKTEKLLKKIEEQEI
                     EESKDKKKGGVLKRKVVIKEEDLYSFRRQRPKTGSFRKDRRDRGGDRRVEEERKAEPK
                     VAKRVVKVSDEITVGELAKRMNVKAQDIIIKLLPMGVMATVNKTIDYDTAYLAATEFG
                     YDVEKIVSIEEEFLAREEEEKDNLEDLKPRSPVVTIMGHVDHGKTLLLDTIRDTNVAE
                     REAGGITQHIGAYKVNVDGKEIIFVDTPGHEAFTAMRARGALVTDIVILVVAADDGVM
                     PQTVEAINHARSAEVPIIVAINKIDKQNANIDKVTKELAEQNLIPEDWGGTTLFAKIS
                     AKKKEGIKELLELIVLQSEMLELKANPDKLARGTVIESELDRGHGPVGTVIIQDGTLK
                     IQDPFISGMTFGRVRAMIDDKGARIQVAPPATPVLVVGFQDVPHGGDRFIVTTEERYA
                     KELSKFRQEKLREREALKSARTTLEDLYSKIGETEKVFLNVIVKGDVRGTIDAIVEAL
                     KKMSTDAVEVQITHSGVGAITETDVNLAMASGSIIIGFNVRPVAKAQALAEQEKIEVR
                     QYSIIYDMIDDVKKAMEGMLAPKIVETTIGKADVRKTYSVSRIGIIAGSYVTEGKATR
                     NSLIRVVRGGQIIHTGKIASLKRFKDDVKEVQSGYECGISLEDYNDIQEGDVFEFFIE
                     EKEKQTLDG"
     gene            complement(2697..>3467)
                     /locus_tag="PKJ17_04450"
     CDS             complement(2697..>3467)
                     /locus_tag="PKJ17_04450"
                     /inference="COORDINATES: protein motif:HMM:NF019275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOD75253.1"
                     /translation="PEIQEGIIKIIGAAREPGERAKISVYSEDPDIDPIGACVGVKGS
                     RVQSIVQELRGEKIDIIPYSRDIAKFVCNTLAPARVSKVYINEEEHSMEIIVNDDQLS
                     LAIGKKGQNVRLASKLTSWKIDISSESEVEKTSKKIIDELVENLRVSEILARILHDEY
                     LRDPKDIAKLTPEEMNKITSISVEDCRRIIDEAKAIMTKKALEAEEAKAAEEAAAEKA
                     PQLTETEEKEGGAEEPAQEARQELEAKPDEETTTPAGE"
BASE COUNT          493 a         1112 c          922 g          940 t
ORIGIN      
        1 ttgtctttga gagagtgatt ctcaggaaag aaaatgtcga cgcgggatac accaactacc
       61 ataaccttca accatcaagg gtctgcttct ccttttcctc gataaaaaat tcgaagacat
      121 ccccttcctg tatgtcgttg taatcctcga gggaaatacc gcattcgtat cccgactgga
      181 cttccttgac gtcatcctta aagcgcttca gggaggcgat cttccccgta tggatgatct
      241 gtccgccccg gacgaccctg atcagcgagt tgcgcgtcgc cttgccctcg gtcacgtagg
      301 aaccggcaat gatgccgatg cgggacacgg agtatgtctt ccgcacgtcc gccttgccta
      361 tcgtggtctc cacgatcttc ggggccagca tgccttccat tgccttcttc acatcgtcta
      421 tcatgtcata gatgatggag tactgcctga cctctatctt ctcctgctcc gccagcgcct
      481 gcgccttcgc gacgggccgc acgttgaacc ctatgatgat ggagcccgac gccatggcaa
      541 ggttcacgtc ggtctccgtg atcgccccga ccccgctgtg ggttatctgg acctcgacgg
      601 catccgtgga catctttttc aacgcctcga cgatcgcgtc gatcgtaccg cgcacatcac
      661 ccttgacgat gacattgaga aacaccttct ccgtctcgcc gatcttcgaa tagagatctt
      721 ccagggttgt cctggcgctc tttaaggcct ccctctcgcg gagcttctcc tgcctgaact
      781 tcgagagctc cttggcgtag cgctcctccg tggtgacgat gaagcggtcc ccgccgtggg
      841 gcacgtcctg gaaaccaacg acgagcacgg gggtcgccgg cggggcgacc tggatcctgg
      901 ctcccttgtc gtcgatcatg gcccgcacgc ggccgaaggt cattcctgag atgaaggggt
      961 cctgtatctt gagggtgccg tcctggatga tgacggtccc caccggtccg tggcccctgt
     1021 cgagctcgct ctcgatgacg gttccccggg ccagcttgtc cgggttcgcc ttgagctcca
     1081 gcatctccga ctgcaggacg ataagctcca gcaactcttt gatgccctct ttcttcttgg
     1141 ccgatatctt cgcgaagagc gtcgtgccgc cccagtcctc gggaatgagg ttctgctcgg
     1201 caagctcttt cgtgaccttg tcgatgttgg cgttctgttt gtctatcttg tttatcgcga
     1261 cgatgatggg aacctcggca ctgcgggcgt ggttgatggc ctccacggtc tgcggcatga
     1321 cgccgtcatc ggccgcgacg acgaggatga cgatgtccgt gacgagcgca ccgcgagcgc
     1381 gcatggcggt gaaggcctcg tggcccggcg tatcgacaaa gatgatctcc ttgccgtcaa
     1441 cattgacctt gtacgcaccg atatgctggg tgatgccgcc ggcctcgcgc tccgccacgt
     1501 tcgtatcccg tatggtgtcg agaagaaggg tcttgccgtg gtcgacgtgg cccatgatgg
     1561 tgacgacggg cgaacgcggc ttcaggtctt ccaggttgtc cttttcctcc tcttcgcggg
     1621 cgaggaactc ctcctcgatg gagacgatct tctcaacgtc gtagccgaat tccgtcgccg
     1681 ccaggtaggc ggtgtcgtag tcgatggtct tgttcaccgt cgccatcaca cccatcggaa
     1741 ggagttttat gatgatgtcc tgtgccttga cgttcattct cttcgcgagc tcgccgacgg
     1801 tgatctcgtc gctcaccttc accacccgtt tcgcgacctt cggttcggcc tttctttctt
     1861 cctcaacgcg cctgtcgcct ccgcggtccc tcctgtcctt tctgaaggaa cctgtcttgg
     1921 gcctctgtct cctgaaggag tacaggtctt cttccttgat gaccactttc ctcttcagga
     1981 caccgccctt cttcttgtcc ttggactcct ctatctcctg ctcctcgatc ttcttgagga
     2041 gcttttccgt cttcttcttc tgacgctccg gcccttcttc ctcgccctca ccttccacag
     2101 cggtgaagtc cttttcgagg tcctgcttgt acgcctcttc gagggccttc gttatctcct
     2161 tctccttctc ttccgcgata tgttctatat cgcccttggg ggccttgtcc tcgggggtaa
     2221 gtatcacggc ctcgggaatc tccggaggag gcgcttcctc gggctgccgt tcctctatat
     2281 gaacatggag gtcctcaggg atcttcctgg gtgtctcctc ggcgatctcc tcttccagtc
     2341 gcggggcttt cggtgccctt tttcttgtcg gaaccttcgt gccggcggcc tcggccttct
     2401 tctcctcttc ggccccgggc ttctcctcta tcggttcttc cttggctttc gggggcggct
     2461 gcaccctccg cctgatgatc gtggaagcga cccttgtttc aacgagggtc tcccccgtcg
     2521 cggaggtctg ttccttcacc gcttcgtgcg ctgcctcgcc ctccttcggt ttgtccttga
     2581 tcttgacacc gatcttcttg agtttggcga gtatctcatc gtcgctggcc ttgtcggtaa
     2641 gggccgtaat cttgattttc gtcattgact gatccttcct tgctcccccg aggcggttat
     2701 tcgccggccg gtgtggtcgt ttcctcgtcc ggcttcgcct ctaattcctg ccgcgcttcc
     2761 tgcgccggct cttcggcacc tccctctttc tcctcggtct ctgtaagctg cggagccttt
     2821 tcggcggcgg cctcctcggc cgctttcgcc tcctcggcct cgagcgcttt tttggtcatg
     2881 atcgccttcg cctcatcgat gatgcgcctg caatcctcca cggatatgct ggtgatcttg
     2941 ttcatctctt cgggcgtcag cttcgcgatg tccttgggat cgcgcaggta ttcgtcgtgg
     3001 aggatccggg caaggatctc ggacaccctg aggttctcca cgagctcgtc gatgatcttc
     3061 ttcgatgtct tctcgacctc ggactcgctg cttatgtcga tcttccagct cgtcagcttc
     3121 gaggcaaggc ggacattctg ccctttcttg ccgatggcca gtgaaagctg gtcgtcgttg
     3181 acgatgatct ccatcgaatg ctcttcctca tttatataca ccttcgacac cctggcaggt
     3241 gccagcgtgt tgcagacgaa cttcgcgatg tctctgctgt aagggatgat atcgatcttc
     3301 tctccgcgga gttcctggac gatgctctgg acccgggacc ccttgacgcc gacgcaggcc
     3361 ccgatggggt cgatgtcggg gtcttcgctg tagacggata tcttggcacg ctcccccggt
     3421 tcccgggcgg cgcctatgat cttgatgatc ccttcctgta tctccgg
//
LOCUS       DAOPOA010000161         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_29516_length_3464_cov_3.016134, whole genome shotgun sequence.
ACCESSION   DAOPOA010000161 DAOPOA010000000
VERSION     DAOPOA010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29516_length_3464_cov_3.016134"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PKJ17_04455"
     CDS             <1..774
                     /locus_tag="PKJ17_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOD75254.1"
                     /translation="GDGHAEELIGRAIRGKRDRIFLATKCGLDIYDRSYRRDLSPAYI
                     EKDLHGSLKRLGTDCVDLYQCHWPDPQVPVEETMAALTRFRDEGKIRHIGVSNFNGPQ
                     LLEALKFVALFSLQPHYSLLERSAGEDLLPICIGKNINVFPYGSLGAGMLTGKYRECP
                     RFKKGDARSFFYRYFKPAYWPRVRRLLDEVEAIAARGGTTSGAVALSWLLRQPGVTSV
                     IVGARSAEQVRGNIADVPVDLSDEDMRALDTLSSLIYES"
     gene            1122..1880
                     /locus_tag="PKJ17_04460"
     CDS             1122..1880
                     /locus_tag="PKJ17_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DtxR family transcriptional regulator"
                     /protein_id="HOD75255.1"
                     /translation="MMAADALTASLEDYLEAIFHIIAEKHAVKPRDIAKRLKVSYASV
                     TGALRSLSEKKLINYSPYDLITLTDEGEVAAKDVVRRHEILHDFFVEVLAVPEADADA
                     AACQMEHSIPRDIVERFVRFVEFVETCPRGGQKWIKGFGYQCDHSNTQENCEKCIASC
                     LEEVRGRIKEGGARAMVSSRLRDVKPGQKCKVVKVRGRSEAGRRIVEMGVTPGSVIEV
                     ERIAPLGDPIDVKVKGYHLSLRKDEAEGIDVELI"
     gene            1867..2130
                     /locus_tag="PKJ17_04465"
     CDS             1867..2130
                     /locus_tag="PKJ17_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOD75256.1"
                     /translation="MSLSEREKSIPGEGQGRPLSAIESGKKVYIVAVDSGRGLQGRLA
                     SMGLVPGVPVDVILNSPRGPFIVAVKGSRIMLGRGMAGKITVV"
     gene            2217..>3464
                     /gene="feoB"
                     /locus_tag="PKJ17_04470"
     CDS             2217..>3464
                     /gene="feoB"
                     /locus_tag="PKJ17_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOD75257.1"
                     /translation="MGSKMVVALAGNPNSGKTTIFNNLTGSRQHVGNYPGVTVERKEG
                     NIRYDGHEIVFVDLPGTYSLTAYSAEELVARNFIVDEKPDVVVDIIDSSNLERNLYLT
                     VQLMETGAPLVLAFNMSDEARTRGYEFDLDRFSRFFNAPVVRTVGHKGEGMDELMKQI
                     VTVALQEKEPRDTPSVNYGEELEEEIGKMIRLLDEEPLLAEKYGARWLSVKLLENDRD
                     VLKKVSSDAVRAQLAKSSSHVEGILGEGPETIIASSRYGFISGACQEAVHSTVEVLHT
                     ISDRIDTVITNRVLGIPIFLGLMYLVFYLTFTLGDPPMGWIEGLFGWLGGKIGALWPE
                     GSESLLRSLIVDGIIGGVGGVVVFLPNILLLFFAIAILEDSGYMARAAFIMDRLMHRI
                     GLHGKSFIPMLIGFGCSVPAIMAT"
BASE COUNT          750 a          884 c         1095 g          735 t
ORIGIN      
        1 ggagacggcc atgccgagga gcttatcgga agggctatcc gcggcaagag ggacaggatc
       61 tttcttgcca caaagtgcgg cctcgacatc tatgaccgtt cgtacaggcg tgacctttcc
      121 cctgcttaca tcgagaagga ccttcacggc tccctgaagc gtctcggcac cgactgcgtc
      181 gacctgtacc agtgccactg gccggaccca caggtccccg tggaggaaac gatggcggcg
      241 ctcacgaggt tcagagatga ggggaagatc aggcacatcg gtgtatcaaa tttcaacggc
      301 ccccaactcc tggaggcctt gaaattcgtt gcccttttct ccttgcagcc ccactattct
      361 ttgctggaga ggagcgccgg ggaagatctt ctccccatct gcatagggaa gaacattaat
      421 gtctttccct atggctccct gggtgccggc atgttgacgg gaaaataccg tgaatgtccc
      481 cggttcaaga agggtgacgc ccgctccttt ttctaccgtt acttcaagcc cgcgtactgg
      541 ccgagggtgc ggcggctcct ggatgaggtg gaagcgatag ccgcccgcgg gggaacgacc
      601 tccggtgctg tggcgctctc gtggcttctc agacagcccg gagtcacatc tgtgatcgtc
      661 ggtgcacgtt cggccgaaca agtcagggga aacatagccg acgtgccggt ggacttgagc
      721 gatgaggaca tgcgcgcgct cgacacgttg tccagcctaa tctacgagtc ctgaggagac
      781 cgggagggga ccgtcgatgc tttgacccca ggcttcggac catcatcagg aaggtgcagg
      841 tccgcgagcc cttgacccca ttaattcgga tgcgctccgc tgtccccggg gcgcattgtt
      901 tccggcaggg atggcacggg caggttcttc cgctctggcc gcccgtattg cttccatgag
      961 ttcaacgagg accctctggc cttcgacaaa ggccggacgg gggcgcctgg gaccttgaag
     1021 cggggaaaac ggacagggac gcgccgaaac atttttcttg caatgccatt aggaatagtg
     1081 taaagaatta gggcaaccta aacttattag tgggggtgtt tatgatggcg gctgacgcat
     1141 tgacggcaag cctggaggac tacctggagg ccatttttca tatcatcgct gagaagcatg
     1201 ccgtgaaacc gcgggacatc gcgaaacgtc ttaaggtgag ctatgcgtcg gtaacgggtg
     1261 cccttcgttc cctgtctgaa aagaaactga tcaattattc gccttatgac cttataacgc
     1321 tgacggatga aggggaggtc gcggcgaagg atgttgtccg caggcacgag atactgcacg
     1381 atttcttcgt cgaggtactc gccgtcccgg aggcggacgc ggacgctgcg gcatgccaga
     1441 tggaacactc catccccagg gacatagtgg agaggttcgt gagattcgtg gagtttgtcg
     1501 agacctgccc tcggggcggt cagaaatgga tcaagggttt cgggtaccag tgcgaccaca
     1561 gcaatactca ggagaactgc gagaagtgta tcgcctcgtg cctggaagag gtgaggggac
     1621 gtataaaaga aggaggtgcc agggcaatgg tatcgtcacg gttaagagat gtaaagccag
     1681 gtcagaagtg caaggtcgtg aaggtccgcg gacggagtga ggcgggaaga aggatcgttg
     1741 agatgggtgt tacccccggt tccgtgattg aggttgagcg catagcgcct ctgggtgatc
     1801 cgatagacgt gaaggtcaag ggttaccacc tgtcgttgag gaaagacgag gctgagggga
     1861 tagacgttga gcttatctga gcgcgaaaag agtattccgg gggagggcca gggaaggccg
     1921 ctcagtgcca tcgagtccgg aaagaaggtg tacatagtcg ccgtggattc cggacggggt
     1981 ctgcagggga gactggcatc gatgggattg gtccccggag tcccggtgga tgtgattctc
     2041 aactcaccgc ggggaccctt catagttgcc gtcaagggga gcaggatcat gcttggccgg
     2101 ggaatggcgg ggaagatcac ggttgtgtaa aaaaatttgc actcatagtt aggcaagcct
     2161 aacttcattg gtggtaacta attacggcag gacagaacag gaggatgata gagcgtatgg
     2221 gatcaaagat ggttgtggct ctcgcgggaa accctaactc ggggaaaacc accatattca
     2281 acaatctgac gggatcgaga cagcatgtgg ggaactaccc cggggtgacg gtggagagga
     2341 aagaaggcaa tatccggtac gacggtcatg agatcgtgtt cgtcgatctt cccggtacat
     2401 acagtcttac cgcctattct gccgaggagt tggtggcgag gaatttcatc gtcgacgaga
     2461 agccggatgt ggtggtggac atcatcgatt cctccaatct ggaacggaat ctgtatctta
     2521 ccgttcagct catggaaacg ggggcgcctc tcgtgctggc cttcaacatg agcgacgagg
     2581 cccgcacccg gggatatgag ttcgacctcg acagattttc ccggttcttc aacgctccgg
     2641 ttgtgaggac cgtcggtcac aagggcgagg gcatggatga gttgatgaag cagatcgtca
     2701 ccgtcgcgct gcaggaaaaa gagccccgtg acacgccttc tgtcaactac ggggaggaac
     2761 tcgaggaaga gatcggcaag atgatccgat tgcttgacga agagcctctt cttgcggaga
     2821 agtacggagc ccgatggttg tcggtgaagc tcctcgaaaa cgacagggat gtcctcaaaa
     2881 aggtttcatc tgatgctgtg cgcgcccaac ttgcgaagag ttcttcccac gtcgaaggca
     2941 tcctgggcga gggtccggaa acgatcatcg ccagcagcag atacgggttc atctcgggag
     3001 cttgtcagga ggccgtgcac tccaccgtcg aggtcctcca taccatttcg gataggatcg
     3061 atacggtcat cacgaaccgt gtccttggca tccccatctt cctggggctc atgtacctgg
     3121 tcttctacct gacattcacc ctgggggatc ctcccatggg gtggatagaa ggtcttttcg
     3181 gatggctggg gggtaagatc ggtgccctgt ggccggaagg ttcggagagc ctgctcagat
     3241 ccctgatcgt tgacggcatc atcggcggcg tcggcggcgt cgtggtcttc ctgcccaata
     3301 ttctcttgtt gttcttcgcg atcgcgatcc tcgaggattc cggctacatg gcgcgggcgg
     3361 cgttcatcat ggaccgcctg atgcaccgga taggacttca cggcaagagc ttcatcccca
     3421 tgcttatcgg tttcggctgc tccgttccag ccataatggc gacg
//
LOCUS       DAOPOA010000162         3462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_29539_length_3462_cov_3.488993, whole genome shotgun sequence.
ACCESSION   DAOPOA010000162 DAOPOA010000000
VERSION     DAOPOA010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29539_length_3462_cov_3.488993"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..290
                     /locus_tag="PKJ17_04475"
     tRNA            227..290
                     /locus_tag="PKJ17_04475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:273..275,aa:Ser,seq:cga)
     gene            complement(460..903)
                     /locus_tag="PKJ17_04480"
     CDS             complement(460..903)
                     /locus_tag="PKJ17_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD75258.1"
                     /translation="MVKPKRILVPTDFSQHSARAYQRALDVAKSSGAEIVLFHVIDQD
                     IPACTKEYCLEEEEVTRQEKTLLEGAKKRLQKEIETFPVSDLKVSTVVREGVPYEEIL
                     KYQDLNDVDLIVIASHGRSAVAKYFLGSVSSNVLKGAKCEVLLVK"
     gene            1248..1913
                     /locus_tag="PKJ17_04485"
     CDS             1248..1913
                     /locus_tag="PKJ17_04485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HOD75259.1"
                     /translation="MEKTGTRTIHLREQLGITLGIALRVQLRGSKDTWNASLVGMVEG
                     RYLIAEVPPIPGLWVKLHQMNNIIVRYLHDGKAYGFYSTLIAALNEPFRLAFLSYPEK
                     IETVNLRKFQRVPCLIPGAVSMSGVSRRGVFTDVSEGGCSFLLDQSGSAPDLGVNIGD
                     EIVFSVRLIGSSDERSVKAIVRNIRTAEKGTSIGTQFHEPGDDLLDSIRTYMGKVGNL
                     DVP"
     gene            2068..>3462
                     /locus_tag="PKJ17_04490"
     CDS             2068..>3462
                     /locus_tag="PKJ17_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD75260.1"
                     /translation="MKRWLFLTSVLVFVIACFYLFFTTYQQVRNDAIEELNKQQTAHA
                     RQAKAAIEGFFQQYFKMLEHLARHDEIINFNKVGKHTIEEFYEASRNDIRSITRIDAA
                     GRFLYTVPDNEKYRGTSVSHRDFFQAMKLDSKPVISDILTTVKGEERSMALHYPVIRK
                     GRFDGSVAVVVSVDDLAGKYLKPIRIGQSGYAWVLNKSGQELYCPVPGHIGVSIYETS
                     GRFPSVIAMAEEMMKGKAGTTTYDYDYIAEKETKKIVKHAVYMPIKIGDTFWSIVVAT
                     PEDEALAAMKGFRDRWFAIVGLLVTGLLVFVFYTVKSWAVLKEAHRRMEAEEASLESE
                     ARYRSIFENAVEGIYQSTPEGRFISVNPAFARIAGYDSPGELIQAVTDISRDVYADPE
                     ERRVFEATLERQGIVTNQEARFRRRDGKVIWVSLNGRAVKDKDGKVLHYEGTVEDITD
                     RKIAQIMIRDEMEFN"
BASE COUNT          777 a          917 c          998 g          770 t
ORIGIN      
        1 cggcataata ggtgatataa tatgaccgat caccggactt catggccctg gtgaacaccc
       61 cttgatactt tgtcttttca tatccttctg gcatataatt tttacccgta ttacccccgt
      121 gtagtcaaaa acagcatcac gtgcggtaaa ttagattttg tacagaaacg ctgtaaacac
      181 tatattttat gcggtaatac gaagtcaagg caaatgaggg aaaacagcgg aaaatgggtc
      241 aaaaaggact tctaatccgt aggtcgcacg ttcgagtcgt gccaggcgcg cttctatccc
      301 gctaatatac cggttttttt caacgcttgt ctgcaatctg cttgcggtca cgggcccgga
      361 gttttcggga cgtcgatacc cgcgttgcat cggcttcggc tcgggaggat cacagccggg
      421 atcatgtttg aaaggcaccc gggcatgggc cgttgatcct cattttacca ggagcacctc
      481 gcatttggcc cctttcagga cgttggaact gacgctcccg aggaaatact tcgcgacggc
      541 ggacctgcca tgggaggcta tgacgatgag atcgacatcg ttgaggtcct ggtatttcag
      601 gatctcctcg taaggcactc cttcccggac caccgtggag acctttaggt cgctgacagg
      661 aaacgtttcg atctccttct gaaggcgttt cttcgcgccc tcgagaagtg tcttctcctg
      721 cctggtcacc tcctcttcct caaggcagta ttccttggtg caggcgggaa tgtcctggtc
      781 gataacgtgg aagagcacga tctccgcgcc ggagcttttt gccacgtcca gagccctttg
      841 gtaggccctt gctgaatgct gtgaaaaatc cgtgggcacc agaatgcgtt ttggttttac
      901 cataactcct ccttatgcgt tttcttgcac gctgaaaaga ccctatatcc gctggatacc
      961 cttgtcaatg ctgcatccct gtcaggcgct ggcatgattg gcgattcggc ggccgtataa
     1021 tggccctttt ttttgcggga tgtcgggggc ttgccccgca tgctcccgtg gaaagcggca
     1081 attgcgcgag cgggttgacg gttgcccgcg gatgccctgg tcacgcgcgc taccctgaca
     1141 gcctccgcgc cgcatcccgg cacaggacag gtcgcttaat gaaatcggag gaatggtcga
     1201 tatactcttc aaggaacccc ggaagaccgg ggggctggat tttcgccatg gagaagacag
     1261 gaacccgaac aattcatctc agggagcaac tcggcatcac gctcggcatt gccctgcgcg
     1321 ttcagctgag agggtcgaag gacacgtgga acgcttccct cgtgggcatg gtggagggcc
     1381 ggtatctcat cgccgaggtg cctcccatac cgggtctctg ggtgaagctc caccagatga
     1441 acaatatcat agtccgctat ctccatgacg gaaaggccta cggtttctat tccacgctca
     1501 ttgccgcctt gaacgaaccc ttccgcctcg ctttcctgtc gtacccggag aagatcgaga
     1561 cggtcaacct gagaaagttc cagagagtgc cctgtcttat ccccggcgcg gtttcgatga
     1621 gcggagtatc gcgacgcggt gttttcaccg acgtgagcga gggcgggtgc agcttcctcc
     1681 tggaccagtc ggggtccgcg ccggacctgg gcgtgaacat cggtgatgag atagtctttt
     1741 cggtgcgcct catcggttcg tcggatgaaa ggtctgtcaa ggccatagtg aggaacatca
     1801 gaacggccga gaaagggaca tccatcggca cgcagtttca cgagcccggc gatgaccttc
     1861 tcgattccat ccgcacatac atgggaaagg tgggaaacct tgatgtgccg tgatggaaaa
     1921 tccacggagg gcgtcgaatg tgattggctt ttcgcgaaaa gttatttaga attcttgtag
     1981 tatcccacac caactgcgtg ccctggaata gcgaagttat ggattggatt tccgcagggg
     2041 ttgtatgcgg ggaaggagga aaaggctttg aaacgctggt tgttcctgac ctccgttctg
     2101 gtcttcgtga tcgcctgttt ttatctcttt tttaccacct accaacaggt caggaatgac
     2161 gccatagagg agctcaacaa acaacagacg gcccatgcca ggcaggccaa ggcggctatc
     2221 gaaggttttt tccagcaata cttcaagatg cttgagcatc tcgcccggca cgacgagatc
     2281 atcaatttca acaaggtcgg caagcacacc attgaggaat tctacgaggc tagcaggaac
     2341 gatatacggt ccatcacccg catcgatgcc gcgggcaggt tcttgtacac tgtgcctgac
     2401 aacgagaaat accggggaac gagcgtatcc catcgggatt tctttcaggc aatgaagctt
     2461 gactcgaaac cggtcatcag cgatatcctc accaccgtca agggggagga gaggtcgatg
     2521 gccctgcatt accccgtgat caggaagggg aggttcgatg ggtccgtggc ggtcgttgtc
     2581 tccgtcgatg accttgccgg gaaatatctc aagccgatcc gtatcgggca aagcggttac
     2641 gcctgggtct tgaacaagag cggccaggag ctgtactgtc ccgtgcccgg acatatcggt
     2701 gtgtccatat acgaaacgtc gggccgcttt ccgagcgtca tcgccatggc ggaggagatg
     2761 atgaagggaa aggcggggac caccacctac gactatgatt atatcgctga aaaggaaacg
     2821 aagaagatcg tgaagcacgc ggtctacatg ccgatcaaga taggtgacac cttctggtcc
     2881 attgtcgtgg ccactcccga agacgaagcc ctggccgcca tgaaagggtt ccgtgacagg
     2941 tggttcgcca ttgtcgggct cctcgtcacg ggccttctag tgtttgtgtt ctatacggtc
     3001 aagtcctggg ccgtcctcaa ggaagcgcac aggcggatgg aggccgagga ggcgtcgctg
     3061 gagagcgagg ccaggtaccg gtcaatcttc gagaacgccg tggagggaat ataccagtcg
     3121 acgccggaag ggcgcttcat aagcgtcaac ccggcgtttg cgaggatcgc cggctacgac
     3181 tccccgggag aattgataca ggccgtcact gacatcagca gggacgttta cgccgatcct
     3241 gaagagcgac gcgtctttga agccaccctc gaaagacagg ggatcgtgac aaaccaggag
     3301 gccaggttca ggcgacgcga cgggaaggtc atatgggtct ccctgaacgg gcgcgcggtg
     3361 aaggacaagg atggaaaggt actccattac gaaggtaccg ttgaagatat aacggaccgc
     3421 aagatagccc agatcatgat ccgggacgag atggagttca ac
//
LOCUS       DAOPOA010000163         3458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_29593_length_3458_cov_3.752277, whole genome shotgun sequence.
ACCESSION   DAOPOA010000163 DAOPOA010000000
VERSION     DAOPOA010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29593_length_3458_cov_3.752277"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PKJ17_04495"
     CDS             complement(<1..504)
                     /locus_tag="PKJ17_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75261.1"
                     /translation="MTAEIRTNYAEFEREWTHSTKAIDKAASSAVKTVAYKTMQDLRR
                     DIRQGRAGSNVFAPLREISKHPAMRQAFWNAGGGKFTRVPRNKPLWTLSKVTRYQVKE
                     SRDTLFAYEVGFVGPSISGNWKEIAMRQQAGNTYTVDSALRKKLFSIGAALKKRKDPA
                     YRYFFVQA"
     gene            complement(501..842)
                     /locus_tag="PKJ17_04500"
     CDS             complement(501..842)
                     /locus_tag="PKJ17_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75262.1"
                     /translation="MTTLSATISSDLAVLLNTDEFAQEITYEGATIKAIVHYGPRRIR
                     GTDQAVSCDAWLEGVKVSDVAVPAYRDTVVIGSTTYHVLIEDAAQPEGDGYTWVIDLR
                     RDERPIPGGRR"
     gene            complement(845..1294)
                     /locus_tag="PKJ17_04505"
     CDS             complement(845..1294)
                     /locus_tag="PKJ17_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75263.1"
                     /translation="MKKLILFAIILLILAPVMAMGAGTATYTITQPDQKTAIVAIAVT
                     DDTAGTTLSLTGLEGYFLCSMETNPGTTAPTDDYDIVINTAGGADLLGGAGANRDTAN
                     TEIAYPTVDSTSGQKGCVPINSTLSFALSGNSVSSATVAVKLFLMRP"
     gene            complement(1291..2268)
                     /locus_tag="PKJ17_04510"
     CDS             complement(1291..2268)
                     /locus_tag="PKJ17_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75264.1"
                     /translation="MVLLGLFGLAFVNWGDNVLRGQTYEFIVLGLMAVSLLLGDAVLS
                     ILGIYLSCWFAYLMWAGFYGAIPAEILFQSLDTMTFIFAGMGVYVLVRRGRMPTERFM
                     NWICGLTVAFCLLALTQMGLFKMDPVATLGNRNFFAAWLAIAMPLFFGKRWLWFIPLI
                     VICLLCAKTSTAIAAALMATAFYLWGWKGAGLAVIPGVAYYLVFKLPVHNLSLGTRLE
                     YWTDALSKMANSWQTILFGVGPGVYWRWGNEFHSEFVYILFNLGIVGLMIMAAYTFRS
                     FRKPCDRKLQAAFLAALIDSFGNHVMHVALTALLTIVILALKDRTEGAT"
     gene            complement(2324..2926)
                     /locus_tag="PKJ17_04515"
     CDS             complement(2324..2926)
                     /locus_tag="PKJ17_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75265.1"
                     /translation="MKKTLFVIAFILLAMATVCHAQTVSQFPNGISTGKVYDYDTSSG
                     WVGSYYMDVPTLSANDTFVGATTTQTLTNKTLTSPTMTSPTISGTTTVTGGTFSGSTL
                     SGAFLSISSKVLSLADWYLSAADKLITFWTLTSGSNGTNYMIACSDTEGRLRIIRNGT
                     NGSVVIKEAGQTGVTIATGKTAVVIHNGTDYVRVSGDATH"
     gene            complement(2954..>3458)
                     /locus_tag="PKJ17_04520"
     CDS             complement(2954..>3458)
                     /locus_tag="PKJ17_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75266.1"
                     /translation="PSGTRVANNAFVDETDLENARVRLAAMKNNRGKRITVPQSEIMV
                     LCPDAIVGKVLKVINSEYVPGVENEVSNWGPRGKWRIPPERVISSPKLDDLSASAWYY
                     GAPRRQFMRKWKLRMEYVTLGADTEAYLKRRVAFQARIAWDVEVGATDYVYWVQNLSA
                     TTSPKDE"
BASE COUNT          738 a          960 c         1022 g          738 t
ORIGIN      
        1 ggcctgaaca aagaagtaac ggtatgccgg gtctttcctt ttcttgaggg ctgcgccgat
       61 ggagaacaac ttttttctca aggcagagtc gacggtgtag gtattgcccg cctgttgcct
      121 catcgctatt tccttccagt tgccggagat ggacggaccg acaaaaccga cctcataggc
      181 gaaaagggtg tccctgctct ccttgacctg ataccgcgtt accttgctca gggtccacag
      241 gggtttgttt ctggggacgc gggtgaactt accgccgcct gcgttccaaa acgcctgccg
      301 catcgcggga tgcttcgata tctcgcggag aggagcaaac acgttcgacc ctgccctgcc
      361 ctgcctgata tccctgcgca agtcctgcat ggttttgtaa gcaacggtct tgacagcgga
      421 tgaggccgcc ttgtcgatgg ccttggtcga atgggtccat tccctctcaa actcggcata
      481 gttggtacga atctcggctg tcatctgcgt cccccgggaa taggtctctc atcccttctc
      541 aggtcgatga cccatgtgta accgtcgccc tcgggctgtg cggcatcctc aatgagcacg
      601 tggtaggtcg tagagccgat gactaccgtg tcacgatagg cggggaccgc cacatcggag
      661 accttcacgc cctcaagcca tgcgtcacag gacacagcct gatcggttcc cctgatgcgg
      721 cggggaccgt aatggacaat agcctttatc gtcgcccctt cataggtgat ctcctgcgcg
      781 aactcgtcgg tgttgaggag aaccgcgagg tcggaggaga tcgttgcaga aagcgtggtc
      841 atagttacgg cctcatcaga aacagcttca ccgcgaccgt ggcactgctc accgaattgc
      901 cggacagggc aaaagagagc gtcgagttaa tcggcacaca ccctttctgg ccggacgttg
      961 aatcgaccgt gggataagcg atctctgtat ttgcggtgtc cctgttcgcg ccggcgcctc
     1021 cgaggaggtc cgcgccgccc gctgtgttga tgacgatgtc gtaatcgtcc gttggcgccg
     1081 tggtgccggg gttggtctcc atcgagcaaa ggaagtagcc ctcgagaccc gtaagactga
     1141 gggtcgtgcc agccgtatcg tcggtcacgg caatagccac aatcgccgtt ttctggtccg
     1201 gttgtgtgat tgtatatgtt gctgttcccg cccccatggc catgaccggg gcaaggatga
     1261 gcaggataat tgcgaaaagg atgagttttt tcatgttgcg ccctccgttc tatctttcag
     1321 ggcaaggata acaatggtca gcagtgccgt cagggccacg tgcatcacgt ggttgccgaa
     1381 ggaatcaatg agtgcggcga ggaaggccgc ctgcaatttc ctgtcgcaag gctttcggaa
     1441 tgacctgaat gtataggcgg ccatgatcat caggccgacg atgccgaggt tgaaaaggat
     1501 gtagacgaac tcggagtgga attcgttgcc ccatctccag taaactcccg ggcctacccc
     1561 gaaaaggatt gtctgccagg agttggccat cttcgaaagc gcgtccgtcc agtattccag
     1621 gcgggtcccc agggaaaggt tgtggacggg gagtttgaac acgagataat aggccacacc
     1681 ggggatgacc gccaggccgg cgcctttcca tccccaaagg taaaaggcgg tcgccataag
     1741 tgccgccgct attgccgtgc tggtttttgc gcagagcaaa cagatgacga tcaaagggat
     1801 gaaccacagc cacctcttcc cgaagaagag gggcatcgct attgcgagcc acgccgcgaa
     1861 gaaattacgg ttccccaggg tggccacggg gtccattttg aaaaggccca tctgcgtgag
     1921 cgcgaggagg caaaaggcaa ccgtcagccc gcatatccag ttcatgaaac gttccgtcgg
     1981 catccggccc ctacgaacga ggacatagac gcccattccc gcaaagatga aagtcatggt
     2041 gtcgagcgac tggaagagga tctccgcggg gatagccccg taaaatccgg cccacataag
     2101 gtaggcgaac cagcaggaga gatagatccc gagaatggac aggaccgcgt ctcccaggag
     2161 gagagacaca gccatcaggc cgagaacgat aaactcgtag gtctggcccc tgaggacatt
     2221 gtcgccccaa ttcacgaagg caagcccaaa gagacccaaa aggaccaagc tctccttgtg
     2281 gctgaaaagg gagagaaggg aggctttcgc cccccttatt gttttaatga gtcgcgtcac
     2341 cggacaccct cacgtaatcg gtgccgttgt ggatgaccac agcggttttg cccgtggcga
     2401 tggtaacgcc cgtctggccg gcttccttga tgacgacaga gccgttcgtg ccgttgcgga
     2461 tgatgcgcag cctgccttcg gtgtccgaac aggcgatcat atagtttgtc ccattgctgc
     2521 cggacgtcag ggtccagaag gtgatgagtt tgtccgctgc ggagaggtac cagtccgcca
     2581 gagaaaggac cttgctgctt atggacagaa aagcgccgga aagcgtggac ccgctgaaag
     2641 tgccgccggt gacagtggta gtacccgaga ttgtagggct tgtcattgtc ggacttgtca
     2701 gcgtcttatt ggtaagagtc tgcgttgtgg tggcgccaac aaacgtgtcg tttgctgaga
     2761 gcgtcggaac gtccatgtaa taggagccga cccatccgct ggaagtatca tagtcataaa
     2821 ccttgcccgt ggagatcccg ttggggaact ggctgacggt ctgtgcgtga cagaccgtcg
     2881 ccatagcaag gagaatgaag gcaattacaa aaagagtctt cttcatgatc gtaccctcct
     2941 tccctttacg gggttattcg tccttcgggg aagtcgtggc cgaaaggttc tggacccagt
     3001 agacgtaatc ggtggcgccg acttcgacgt cccatgcgat acgcgcctga aaggcaacgc
     3061 gcctcttaag ataggcttcc gtgtccgctc cgagggtcac atactccatg cggagcttcc
     3121 atttccgcat gaactgacgc ctgggggcac cgtagtacca ggccgacgcc gacagatcgt
     3181 caagcttcgg ggagctgatg accctttccg ggggaatccg ccatttaccg cgggggcccc
     3241 agttggaaac ctcattctcg acgcccggaa catattcgct attgatgacc ttgaggacct
     3301 tgccgacgat ggcatcaggg cagagaacca ttatctcgct ctgaggcacg gttatccttt
     3361 tgccccggtt gttcttcatt gcagcgagtc tcacgcgggc gttctcgagg tcggtctcgt
     3421 ccacaaaggc gttgttcgct accctcgtgc cggacggg
//
LOCUS       DAOPOA010000164         3454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_29649_length_3454_cov_2.641365, whole genome shotgun sequence.
ACCESSION   DAOPOA010000164 DAOPOA010000000
VERSION     DAOPOA010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29649_length_3454_cov_2.641365"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1416
                     /locus_tag="PKJ17_04525"
     CDS             139..1416
                     /locus_tag="PKJ17_04525"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOD75267.1"
                     /translation="MKTLIRGGRLVDPKNSIDGRYDVLVDGPVVKEVSKSIRKVDDDT
                     RIIDASKRIVAPGLIDVHCHLREPGFEYKETIRTGTTAATRGGFTTVVCMANTNPVND
                     SRSVTEFIVDKARAEGVCKVLPCGAVTRGLRSEELSEIGEMFEAGIVAISDDGRSVRA
                     AGLLRKALEYAKIFKLPVISHCEDETLSGGCVHEGRASLLSGLDGIPAIAEELIAGRD
                     IAVARYVDAPLHLTHISTAGSVEIIERAKRTFRKITCDTCPHYFTLTDEATLTYDTNT
                     KVNPPLRSQDDVNAIKEGLRKGTIDIIATDHAPHEFTSKDVEFDLATFGISGFETALA
                     LSLGLVREDILDLKGLLMKMTANPAKLLGLEAGVISEGAPADIIVFDPDMEWTVDRKS
                     FASKGKNTPFHGMTLRGRNLFTMVDGKIVYNEL"
     gene            1702..2556
                     /gene="motA"
                     /locus_tag="PKJ17_04530"
     CDS             1702..2556
                     /gene="motA"
                     /locus_tag="PKJ17_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HOD75268.1"
                     /translation="MFVIIGAIVVLGAVFGGFVLEGGPLKLIVQPVEFLIIGGAAVGA
                     MVISARKKLLMKIVAKVMASLKGGAINRELYLDLLRLMYEIFQVTRKDGLIALESHIE
                     HPDQSKIFTKYPNITRHHHIISFMTDTFRLIVLGGIAPHDIESLMDLDIDTHHQEGAQ
                     PGMILQKIGDSMPGLGIVAAVLGIVITMQAIGGPAEEVGHKVAVALVGTFLGIFLSYG
                     FIQPMATNLDLAAEEESKVFEAIKSGIISLAKGFNPIVSVEFARRSIPNDYRPTFQEM
                     ESFVKGVK"
     gene            2559..3410
                     /locus_tag="PKJ17_04535"
     CDS             2559..3410
                     /locus_tag="PKJ17_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOD75269.1"
                     /translation="MDDHQTPVRIIVKKKRGHGGHHGGAWKVAYADFVTAMMALFIVL
                     WIVGQSEAVKQAVSAYFKDPGVFESGRGGSVLQGSTGSAPNPTTDVSSEIERLKVEAK
                     KLEAEIRSNPEFDKFRDKIEIAVTKEGLRIELLENSNGLFFDVGSAKLKPETVKLLKL
                     IAVEVGHLKNKVMIEGYTDARPYVHPDYTNWELSADRANAARRILEQSGVGKDQIVQV
                     RGFADRNLKHPDKPLDFGNRRVSILVATTEPRPSDAITPAAPAVTPQKETVAPAAPEV
                     APKIETK"
BASE COUNT          769 a         1017 c         1017 g          651 t
ORIGIN      
        1 gcacccgggg cccatgaacc ggggtatcga gatctccgac gaggtcgctg acggcccatc
       61 ctcggtgatc ctggatcagg tggagaacgg tgtggccgtg cggatggccg tcctttatct
      121 cctcatcgga ggtgaagcgt gaagaccctg atacggggag ggcgccttgt cgatccgaag
      181 aactccatcg acggcagata cgatgtcctt gtcgacggac cggtcgtcaa ggaggtttcg
      241 aagagcatcc gcaaggtcga tgacgacacg aggatcatcg acgcatcgaa gcgcatcgtg
      301 gcgccgggcc ttatcgatgt ccactgccat ctgcgtgagc ccggtttcga gtacaaggag
      361 accatacgga ctggaacgac cgcggcgacc cgcggcgggt tcacgaccgt ggtctgcatg
      421 gcgaacacca atcccgtgaa tgactcgcgg agcgtcaccg aattcatcgt cgacaaagcc
      481 cgggcggagg gtgtctgcaa ggtccttccc tgcggcgcgg tcaccagggg gctaaggagc
      541 gaggaactgt ccgagatcgg cgagatgttc gaagccggca tcgtggcgat ctccgacgat
      601 gggaggtccg tgcgcgcagc cgggctcctg cgcaaggccc tggagtacgc gaagatattc
      661 aagctgcccg tgatatcgca ctgcgaggac gagaccctct ccgggggttg cgttcatgag
      721 ggacgggcct cgcttctttc ggggctcgac gggatccccg ccatcgccga ggaactcatt
      781 gcgggcaggg acatcgcggt agcgcgatac gtcgacgcgc ccctccacct tacccacatt
      841 tcgactgccg gcagcgtgga gatcatcgag cgcgcgaaac ggacgttcag gaagatcacc
      901 tgcgacacct gtccccacta tttcacgctc accgatgagg caaccctcac ctatgacacg
      961 aacacgaagg tgaatcctcc cttgaggtct caggacgacg tcaatgccat caaggaaggg
     1021 ctccgcaagg ggaccatcga tatcatcgcc accgaccacg cgcctcacga attcacctcc
     1081 aaggatgtcg agttcgacct ggcgaccttc ggtatctcgg gcttcgagac ggcccttgcg
     1141 ctgtcgctgg gcctcgtccg ggaggacatc ctcgacctga agggccttct catgaagatg
     1201 accgccaatc cggcgaaatt gctgggcctc gaggccggcg tcataagcga aggagccccg
     1261 gccgatatca tcgtcttcga ccccgatatg gaatggacgg tcgacagaaa gtccttcgcc
     1321 tcgaagggaa agaacacccc cttccacggc atgacgctgc gcggcaggaa cctcttcacc
     1381 atggtcgacg gaaagatcgt ctacaacgaa ctctagagac ggtttcaagt tttaggtttt
     1441 aagttttagg ttgaaaacag aaaaaaaccg gctctattcc cttgaaatac aggcgcatcc
     1501 cgctattctg cctaccctgg tctttcactt aaaacttaaa acctaaaacg taaaaccgta
     1561 tcttaatctt ttcttccctc ttccgataac ctttataccc ggaccgatgg acagcatgat
     1621 atcagcatac tggtccgtaa agggtgatgg accctgtaca ccccgcgaga gagcggggct
     1681 tttgagggca cggaagacac aatgtttgtc attatcggtg cgattgtagt tctgggtgcg
     1741 gtattcgggg gtttcgttct ggagggcggc cccttgaagc ttatcgtgca gcccgtcgag
     1801 ttcctcatca taggcggggc ggccgttggg gcgatggtga tatcggcccg caagaaactc
     1861 ctcatgaaga tcgttgccaa ggtaatggcc tccctcaaag gaggggccat taaccgcgag
     1921 ctctatctcg acctcctccg gctcatgtac gagatcttcc aggtgacgcg caaggacggc
     1981 ctcattgccc tggaatctca tatcgagcac cccgatcaga gcaaaatatt caccaagtac
     2041 cccaacatca cgcggcatca ccatattatc tccttcatga ccgacacctt caggctcatc
     2101 gtcctcgggg ggatcgcccc ccacgacatc gagagtctca tggatctcga tatcgacaca
     2161 catcaccagg aaggcgcgca gccgggcatg atccttcaga agataggtga ctccatgccg
     2221 ggtctcggca tcgtcgccgc cgtcctcggc atcgtcatca ccatgcaggc catcggcgga
     2281 ccggcggaag aggtcggcca caaggtggcg gtcgccctcg tcggcacctt cctgggtatc
     2341 ttcctctcct acggcttcat ccagcccatg gccacgaacc tcgaccttgc ggcggaagag
     2401 gaatcaaagg tttttgaggc tatcaagtcg ggtatcatat cccttgccaa gggtttcaac
     2461 cccatagtct ccgtggaatt cgcccgaagg tcgatcccca atgattatcg gcccaccttc
     2521 caggagatgg agtccttcgt aaaaggggtc aagtagccat ggatgaccat cagactcccg
     2581 taaggatcat agtcaagaag aagagggggc acggcgggca ccacgggggc gcgtggaagg
     2641 ttgcctacgc ggacttcgtc accgccatga tggcactctt catcgttctg tggatcgtgg
     2701 gacagagcga ggcggtcaag caggcggtct cggcctactt caaggacccc ggagtcttcg
     2761 agagcggccg cggagggagc gttcttcagg gttccacggg ttccgcaccg aatcccacga
     2821 cggacgtctc ttcggagatc gagaggctca aggtcgaggc aaagaagctg gaggcggaga
     2881 tcaggtccaa tcctgagttc gacaagttca gggacaagat agagatcgcg gtgacaaaag
     2941 aaggtctgcg catcgaactg ctggagaatt ccaacggcct gttcttcgat gtcggcagcg
     3001 cgaagctgaa gccggagacg gtaaagctcc tcaagctcat cgctgtcgag gtgggacacc
     3061 tcaagaacaa ggtcatgatc gagggttaca cggacgcccg accatacgtg caccccgatt
     3121 ataccaactg ggaactgagc gcggaccggg caaacgcggc caggcggatc ctcgaacaga
     3181 gcggggtcgg caaggaccag atcgtgcagg tgcgcggttt tgccgaccgg aacctcaagc
     3241 accccgacaa acccctcgat ttcggaaaca ggagggtgag catacttgtc gcgacgacag
     3301 aaccaagacc ttcggacgct atcacgcctg cggcgcccgc cgtcacgcct cagaaggaaa
     3361 cggtggcacc ggcggcaccg gaggtggcgc cgaagataga gaccaaatga tggagaaccc
     3421 cgtggtgcgc atccttctcg tggacgatga cggc
//
LOCUS       DAOPOA010000165         3447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_29731_length_3447_cov_4.021816, whole genome shotgun sequence.
ACCESSION   DAOPOA010000165 DAOPOA010000000
VERSION     DAOPOA010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29731_length_3447_cov_4.021816"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="PKJ17_04540"
     CDS             <1..1187
                     /locus_tag="PKJ17_04540"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOD75270.1"
                     /translation="VEPGILEKIKHAKREIVIHFPVKMNDGTVQIFEGYRVQHNDTRG
                     PFKGGLRFHADVHLDEMKALAMWMTFKAAIVNVPFGGAKGGVKCDPRTMTSGELEKVT
                     RRYTWDIAPFIGPEVDILAPDLATNPRVMAWVMDTYSIREGHPVPGVVTGKPLELGGS
                     RGRLEATARGLLIVAERAARLKGMAMKDAKVAIQGAGNVGGTAARLFSRAGARVIAIS
                     DIKGGIHNSRGLDIAKALQCKVSNNCFTKGEIDGDEISNEELLELECDILVPAAIENQ
                     ITEENAGKLRCRMIVEGANGPTTPAADEVLRQKDIYLVPDILANAGGIVVSYFEWVQN
                     LQELLWSEEEVYQKLRRILVRAFDETGALSERHKVDMRTAATILSIGRVAEATRLRGI
                     FP"
     gene            1293..2327
                     /locus_tag="PKJ17_04545"
     CDS             1293..2327
                     /locus_tag="PKJ17_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HOD75271.1"
                     /translation="MDLTDAREKARERMKGACAVYKVCDGRADRLCQGIKYGKALGMG
                     GAGKGASFTANVEALDRLHLKTRLICEHAEPQMKTSFLGQEVGFPIFCSSVSGVKISM
                     GGAIGELEFFRAVIQGCKSAGTIAFVGDTAETFEDRPGVQAIREAGGWGVQIFKPREQ
                     KVLLRVIQEAQAAGAIAVGVDLDGAGSVAMGLKGQPVFRKSLNELKELVESTSLPFIL
                     KGITSEEDAAAALETGCKVVCVSNHGGRTLDSMPGVAEVLPRLVPILKGKVVVTADGG
                     VRTGFDAFKMLALGADFVMAGREFIRAAIGGGAEGVVAEADFLFQDLRKAMIMTGCNS
                     LSDIDGHVFT"
     gene            2349..>3447
                     /locus_tag="PKJ17_04550"
     CDS             2349..>3447
                     /locus_tag="PKJ17_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOD75272.1"
                     /translation="MKRNLVEKILVDHLVEGELKAGGEIGLKIDHTLLQDATGTLAML
                     EFEALGLPAVRAELSVQYVDHNILHTDFKNADDHKFLQTACARYGIIFSGAGNGVSHQ
                     VHMERFGVPGKTLLGADSHTPGAAGVSMLGIGAGGLDVALAMAGFPYYLLNPRVLGVK
                     LLGSLPDWVSAKDVILEMLRRFGVKGCTGKIVEYYGPGVKTLSATDRETIGNMGTELG
                     ATSSIFPSDERTREYLEAQGRGESWRVLSADEGATYDEDLTLDLGAIEPLIAKPSSPG
                     NVVPVREVAGTKVDQAIVGSSVNSSFRDLMVSAKILEGRHVHRTVSFHVNPGSRQVLE
                     NVARESGMLAFLRAGTRVHEPGCHGCIGMGQA"
BASE COUNT          749 a          912 c         1122 g          664 t
ORIGIN      
        1 acgtggagcc cggcattctc gaaaagatca agcatgcaaa acgtgagatc gttattcatt
       61 tcccggtgaa gatgaacgat gggaccgttc agatcttcga gggataccgt gtgcagcata
      121 acgacacgcg gggcccgttc aaaggtgggc tgcggttcca tgccgacgta cacctggatg
      181 aaatgaaggc tctggccatg tggatgacgt tcaaggccgc gatagtcaac gttcctttcg
      241 gcggtgcaaa aggaggcgtg aaatgtgacc cgcgcaccat gacgtccggg gagttggaga
      301 aggtgacccg tcggtacacc tgggacatag cgccctttat cggtcccgag gtggatatac
      361 tggcccctga cctggctacc aatccccggg tcatggcctg ggtaatggat acgtacagca
      421 taagggaggg ccacccggtg ccgggtgtcg tgacaggaaa acctttggaa ctcggtgggt
      481 cccgggggag attggaagca actgcccggg gcctcttgat cgtcgccgag cgagcggcac
      541 gcctgaaggg tatggcaatg aaggatgcaa aggtagcgat acagggtgcg ggcaacgttg
      601 gcggaacggc agcccgtttg ttcagccggg ccggggccag ggtgatcgcc atctccgata
      661 tcaagggcgg gatccataat tcccggggcc tcgacatagc aaaggccctt cagtgcaagg
      721 taagcaacaa ttgtttcacg aaaggcgaga tcgacggtga tgagataagc aatgaggaac
      781 tgcttgaact ggaatgcgat attctcgtgc cggctgccat agagaatcag atcacggagg
      841 agaacgcagg caaactccgg tgccgcatga tcgtcgaggg ggctaacggg ccgacgaccc
      901 ccgctgccga cgaggtgctc aggcagaagg acatttacct tgttcccgac attctggcga
      961 atgccggcgg gatcgtcgtg tcctatttcg agtgggtgca aaatcttcag gaattgctct
     1021 ggagcgagga ggaggtctac cagaagttaa ggagaatact cgtgagggcc ttcgacgaga
     1081 ccggggcatt gtcggaaaga cataaggtag atatgcgaac ggcggcaact atcctcagca
     1141 tcggtcgcgt cgcagaggcc accaggctgc gtgggatatt tccttgagag gcagaccgat
     1201 ggatttgaca gcaacacccc gaagcattaa gttcggctta gcacctcgaa tagggaaagc
     1261 taaaggtcac atgagcagct tcggaggtcc tcatggatct gactgatgcc cgggaaaaag
     1321 caagagaacg gatgaaaggg gcctgtgccg tttacaaggt ctgtgatggc cgtgcggacc
     1381 gtctctgtca gggcatcaaa tacggaaagg cgctggggat gggaggggcc gggaaaggcg
     1441 cttctttcac cgccaacgtc gaggcgctgg accgccttca tctgaaaacc cgtctgatct
     1501 gtgagcacgc tgagccgcag atgaaaactt cttttctcgg gcaggaggtt ggcttcccga
     1561 tcttttgttc ctccgtgagc ggggtgaaga tcagcatggg gggtgcgatc ggcgagctgg
     1621 agttttttcg ggccgtaata cagggatgca agtcggcggg aaccatcgct ttcgtcggag
     1681 ataccgccga aacttttgaa gaccggcctg gagtacaggc aatccgggaa gccggcggct
     1741 ggggagtgca gatcttcaag ccccgggaac aaaaggtttt gttgcgcgtc attcaggagg
     1801 ctcaagcagc cggggcgatc gcagtggggg tcgatctcga cggcgcaggc tccgtggcca
     1861 tgggcctcaa ggggcagccc gtgttccgga agtctttgaa cgagctgaaa gaactcgtcg
     1921 aatccacatc actgcccttt atactcaagg ggatcacgag cgaagaagac gccgcagccg
     1981 cacttgagac cggctgcaaa gtggtctgtg tttccaacca cggcgggcgc accctcgatt
     2041 ccatgccggg ggtcgccgaa gtactgccgc ggttggtccc cattctgaag ggaaaggtgg
     2101 tcgtcactgc ggacggaggc gtccgtaccg gtttcgatgc gttcaaaatg ctcgccctgg
     2161 gagcggactt cgttatggcc gggcgcgaat tcatacgcgc ggccattggc ggtggagcgg
     2221 aaggggtggt ggctgaagcg gacttccttt tccaggactt gaggaaagcg atgatcatga
     2281 cgggctgcaa ctctttatct gatatagacg gccatgtctt cacgtaaggg gtattcaggg
     2341 tacctaccat gaaacgaaac cttgtcgaga aaatactcgt cgatcacctc gttgagggtg
     2401 agctcaaggc cggcggggag atcggcctta agatcgacca caccctgctt caagatgcca
     2461 caggcaccct cgccatgctc gagttcgaag ctctggggct tcccgcggtc agggcggagc
     2521 tgagcgtcca gtacgtggac cacaacatcc tgcacacgga cttcaagaat gccgacgatc
     2581 acaagttcct ccagacagcc tgcgcacggt acggcatcat cttcagcggg gcaggcaacg
     2641 gcgtatccca tcaggtccat atggagcgtt tcggcgtacc cggcaagacc ttgctcggtg
     2701 cggacagcca cacgccggga gccgccggag tctcgatgct cggcatcggt gcgggcgggc
     2761 tggacgttgc gctcgccatg gcaggctttc cgtattatct tctcaatccc cgtgtgctcg
     2821 gcgtgaagct tctgggaagt cttcccgact gggtgagcgc caaggatgtc atcctcgaaa
     2881 tgctgcgtcg tttcggcgtc aagggttgta cgggcaagat cgttgaatac tacggcccgg
     2941 gagtcaagac cctttccgcg acagaccggg agaccattgg caatatgggg accgaactgg
     3001 gcgctacgag cagcattttc ccgtccgatg aacggacgcg ggaatatctc gaagcgcaag
     3061 gccgtggcga gtcatggcgt gtactttcag ccgacgaggg cgcaacgtac gacgaagacc
     3121 ttaccctgga tctcggggct atcgagccgc tcatcgcgaa gccctcctcg cccggcaatg
     3181 tcgtccccgt acgggaagtg gccggtacaa aggtggacca ggcgatcgtg ggcagcagcg
     3241 tcaattcctc cttccgggac ctgatggttt cagcgaagat cctggagggc aggcatgtgc
     3301 atcggacagt ttctttccac gtgaacccgg gcagccgtca ggtgctggaa aacgtggcca
     3361 gggagagcgg gatgctggca ttcttgaggg ccggcacccg ggtacacgag cccggctgcc
     3421 acgggtgcat cggaatgggc caggccc
//
LOCUS       DAOPOA010000166         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_29768_length_3444_cov_3.203895, whole genome shotgun sequence.
ACCESSION   DAOPOA010000166 DAOPOA010000000
VERSION     DAOPOA010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29768_length_3444_cov_3.203895"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1964
                     /locus_tag="PKJ17_04555"
     CDS             <1..1964
                     /locus_tag="PKJ17_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HOD75273.1"
                     /translation="YSGGTTVSEGTLQVGTGNSLPAGTNVILGNAAGAILDLNNFNVT
                     IGSLAGGGTTGGNVTLGTGTLTVGGASGGTYAGVISGTGGLTKTGTGTLVLSGVNTYT
                     GTTTLDGGSLAVTGSISSPVVINPSGVLTSNSNIVGNVTNYGKVAPGNSIGVVTITGN
                     YTHNPGSVYEVETDPDGQSDKLVVTGTATLNGGTVSVLTGSGVYEMRTYYTILTAGNV
                     TGTFANVTSDLACLTPSLSYDANRVYLFLARKFTDFSGVAVTGNQKTVASALDRISTS
                     ARGDMEDVVNAVLKLSAPGARNACEQMGGLSHTALTATTFSCFRGYMGTMTERSRTFI
                     TGQPSSAFAARPVMLAFKGGDRASDAGNTLLAALGRTGGTGSPYRGLWVQGYGGTGDK
                     GGGDAASRYGYDMAGVVAGFDRQVADPFILGISLGYSSTRTSMSDLSDKATVSSYQAG
                     LYGIFRHDPWYLSSAAAFGYNRYDTQRDISFGEIVRSARARYDGHSLGGYLETGYRVE
                     TRGADIIPMVSLMGGRLMRDGFRERNAGAVSLDVDGERSSFLLGALGVRLRKDYAFSS
                     SIFTPEITVRWDHDFFGDDYVLNGRFAGQPLSIFSTSGERGDRDSLAPGFGLTLRTKE
                     NVHFQLTYDGSFTSDSTQHRATLGLRYAW"
     gene            complement(2014..2092)
                     /locus_tag="PKJ17_04560"
     tRNA            complement(2014..2092)
                     /locus_tag="PKJ17_04560"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2054..2056),aa:Pro,seq:cgg)
     gene            2189..>3444
                     /locus_tag="PKJ17_04565"
     CDS             2189..>3444
                     /locus_tag="PKJ17_04565"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOD75274.1"
                     /translation="MEKKPVRITDLTLRDGHQSLFATRMRTEDMLPIAEKMDSVGFYS
                     MEVWGGATFDVMTRFLDEDPWERVRALKEKMPNTKLQMLLRGQNLVGYRNYADDVVDA
                     FVEKSCEVGIDIFRVFDALNDERNVIAAFRAIKRCGGHIQGAISYSLTEGRLGGPIFN
                     IGYYLDKAKKIEDMGADSLCIKDMAGIISPYDAYELVSRLKETLSIPVHLHTHYTSGM
                     ASMSLLKAIEAGADGIDTCLAPFAYRSSHSPSEAFAVALKDTPYDPKLDLGKMSEVGD
                     YLEEIIPPKLRSFSDRTRYAVIDIGVLIHQIPGGMISNLVSQLKQAKALDRLEEVYEE
                     IPRTRQDMGFPPLVTPTSQIVGVQAVFNVIAGRYKMISNEVKDYFYGLYGRPPAPVSE
                     EIKAKALKGYSRGTTPIDVRPADILEP"
BASE COUNT          734 a         1009 c         1049 g          652 t
ORIGIN      
        1 cgtattccgg agggacgacg gtgagcgaag gaacactgca ggttgggacc ggcaacagcc
       61 ttcccgctgg aacgaatgtt atcctgggga acgcggcggg ggcgatcctc gacctcaaca
      121 acttcaacgt gaccatcggt tccctcgcgg ggggcggcac gacgggcggc aacgtcaccc
      181 ttggcaccgg gaccctcacc gttggcggcg cgtcaggcgg cacatatgcg ggtgtcatct
      241 ccgggacggg cggtctcaca aagaccggca caggcacgct cgtcctctcc ggggtgaaca
      301 cctataccgg gacgacaacg ctcgatgggg ggtcgctggc ggtgacgggc agcatatcga
      361 gtcccgtggt catcaacccc tcgggggttc ttacgagcaa cagcaacatc gtcggcaacg
      421 tcacgaacta cggcaaggtc gcgccgggga actccatcgg tgttgtcacc atcaccggca
      481 actacaccca taatccgggt tccgtttacg aggtggagac agaccctgac ggacagtcgg
      541 acaagctcgt agttaccggt accgccacgc ttaacggcgg taccgtatcg gtcctcacgg
      601 gatcgggcgt ctacgagatg aggacatatt acacgattct cacggcgggg aacgtgacgg
      661 gaaccttcgc gaacgtcacg agcgacctcg cttgcctcac gccttccctg agctacgatg
      721 cgaaccgtgt ctatctcttt ctcgcccgca agttcacgga tttctcaggc gtggcagtga
      781 cgggaaacca gaagacggtg gcctccgcgc tggaccgcat atcgacatcg gcaagaggcg
      841 acatggagga tgtcgtcaac gccgtcctga aactttccgc gccgggggca aggaacgcct
      901 gcgagcagat gggcggactg tcccacaccg cccttacggc gacgacgttc tcgtgttttc
      961 gcgggtacat ggggaccatg acggaaagat cgaggacctt cataacagga cagccgtcat
     1021 cggccttcgc cgcccgcccc gtcatgctcg ctttcaaggg cggcgacagg gccagtgatg
     1081 ccggtaatac gctcctcgcg gccctcggca ggaccggcgg caccggttca ccctacaggg
     1141 gcctgtgggt gcaagggtac ggcggaacag gcgacaaagg cggcggcgat gcggcgtctc
     1201 gctacggata cgacatggca ggcgtcgttg ccgggttcga caggcaagtg gccgatccct
     1261 tcattctcgg catctccctg ggatactcca gtacgaggac gtccatgagc gatctttcag
     1321 acaaggccac ggtatcgagc taccaggccg gtctctatgg catattcagg cacgacccgt
     1381 ggtacctgag ctccgccgca gccttcgggt acaaccgcta cgacacgcag agggacatat
     1441 ccttcggaga gatcgtaagg tcggcaaggg cacgctacga cggccattcg ctcgggggat
     1501 acctggagac gggttaccgg gtagagacga ggggtgcgga catcataccc atggtgtccc
     1561 tgatgggtgg ccgcctcatg cgggacggct tccgggaaag aaatgccggg gccgtgagcc
     1621 tcgacgtgga cggggagcgc tcctccttcc ttcttggcgc cctgggcgtg agactcagaa
     1681 aggattacgc cttctcttcg agcatcttta caccggagat cacggtgcgc tgggaccacg
     1741 atttcttcgg cgatgactac gttctcaacg gccgctttgc cggacagcca ctttcaatct
     1801 tcagcaccag tggtgagagg ggcgacaggg acagcctcgc cccgggattc gggctcaccc
     1861 tgcggacgaa ggagaacgtg catttccagc tgacctacga cgggagcttc accagtgaca
     1921 gcacgcagca ccgggcaact ctgggcctca gatacgcatg gtaaaacctt tcattgaaaa
     1981 gtggacgttt acgggatatt tccaaggtta caatggtcgg gatgactgga tttgaaccag
     2041 cggcctcttg ctcccgaagc aagcgctcta caccatgctg agctacatcc cgtaggcatt
     2101 aaaataccag agttgccgga aaatatccat tgatttttca cggttgccgt gatactagtt
     2161 ctgactttac tgaagggggt ttgaagccat ggagaagaag ccggtcagga taacggacct
     2221 gacattgaga gacggtcacc aatccctgtt tgccaccagg atgagaacgg aggacatgct
     2281 gcccatagcc gaaaagatgg attccgtcgg gttctactcg atggaggtct ggggcggggc
     2341 aaccttcgac gtgatgaccc ggttcctcga cgaggacccg tgggaaaggg tccgggccct
     2401 gaaggagaag atgcccaaca cgaagttaca gatgctcctt cggggacaga acctcgtcgg
     2461 ctaccggaac tacgccgatg acgtcgtcga tgccttcgtc gagaagagct gcgaggtggg
     2521 catagacata ttccgggtct ttgacgccct taacgacgaa cgcaatgtca tcgccgcctt
     2581 cagggccatc aagcgctgcg gcgggcatat ccagggggct atatcctatt cactgacgga
     2641 agggagactt ggagggccga tattcaatat cgggtattac ctcgacaagg cgaagaagat
     2701 agaggacatg ggcgccgact ctctgtgcat caaggacatg gcggggatca tctcgcccta
     2761 tgacgcctat gagcttgtgt cgcgcctcaa ggagaccctt tcaatacccg ttcatcttca
     2821 cacgcactat acgagcggga tggcctcgat gtcgctcctc aaggccattg aagccggcgc
     2881 cgacggcatc gatacctgtc ttgccccttt tgcgtaccgg tcctcccatt cgccttccga
     2941 ggcctttgcc gtcgcgctca aggacacccc ctacgatccg aaactggacc tcgggaagat
     3001 gtcggaggta ggcgattatc tcgaggagat cattccgccg aagctgcgat ctttttcaga
     3061 ccggacccgt tatgccgtta ttgatatcgg ggtcctcatc catcagatcc ccggtggcat
     3121 gatctcgaac ctggtcagtc agctcaagca ggcaaaagcg ctcgacaggc tcgaggaggt
     3181 ctacgaagag ataccgagaa cgaggcagga tatgggcttt ccgccgctcg tcacgcccac
     3241 gagccagatc gtcggagtgc aggctgtctt caacgtcatc gcgggccggt ataagatgat
     3301 cagcaacgag gtgaaggact atttctacgg tctctacggc cgtccccccg cgcccgtgag
     3361 cgaggagata aaggcgaagg cattgaaggg ctattcccgg ggaacgacgc ccatcgatgt
     3421 gcgcccggcg gacatcctcg agcc
//
LOCUS       DAOPOA010000167         3426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_29952_length_3426_cov_3.732424, whole genome shotgun sequence.
ACCESSION   DAOPOA010000167 DAOPOA010000000
VERSION     DAOPOA010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29952_length_3426_cov_3.732424"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..925
                     /locus_tag="PKJ17_04570"
     CDS             146..925
                     /locus_tag="PKJ17_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOD75275.1"
                     /translation="MNILVFTKRVAATQEEELRIRDEGASVDLSKVPFKINDWDNYSI
                     EEAVRIVEKTGGTVTAISIGDAESDEVLRRAIAMGAKDGFLVNSESVFHDPGVRSRLI
                     QNFIQKENIPFDVIFTGVQSEDDQFGCVGGILGAVLGLPYSSMVIGIDAVEKDHMIIR
                     RELEGGLQERIKITVPCVLSIQSGINEPRYVSIMGIRKASKVERKVFKAADYAAEAGP
                     GIEVVQWAYPEKKGVATMLTGELDVVCGQLLGILKEKGVFQ"
     gene            922..1848
                     /locus_tag="PKJ17_04575"
     CDS             922..1848
                     /locus_tag="PKJ17_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOD75276.1"
                     /translation="MSYALIIAEVRRGVFEERNLDSAGIAALLGKEAVMLLPDGAYQT
                     GNIADTIVKVKVGEEVFLNPLNLKNILEKVFTARGNPDVIIFTHSSFGMESAAYTAGA
                     FGFPVVTDVSGYDKERGVFLKSYYSDKIFGEFKANGGPVVLSVRSGSFKEFAAEKAAA
                     RVEEIEGVAPVEGRTFVEYVEEEKADVDITKADFLLSVGRGIGNKDEIPAYEDLAGKL
                     KAVLSGSRPVIDKMWLPKPRQVGTSGKTVKPKVYLAMGISGAFQHIAGMKDSECIIAV
                     NKDTEAPIFQYAHFGIVADIHKVRDKLKEIMG"
     gene            1848..3152
                     /locus_tag="PKJ17_04580"
     CDS             1848..3152
                     /locus_tag="PKJ17_04580"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOD75277.1"
                     /translation="MDNSGKNVLVVGGGIGGITAALELASCGINVSMVESGPSIGGRM
                     IQLDKTFPTLDCSTCTLSPKMVEVSLNHNIDLMSVSQITHMARKGPGFLVGIKKNPRY
                     VDPKKCTACGTCSEACVMKDRIPHEFNMMLGKRAAIYMPFPQAIPQKYLVDPERCLFV
                     SRGKCKKACMDACETGAIDFEDKGEELKAYFDSIVLAPGFDVYDPTEKQELGYGTLPG
                     VVTGLEFERICSVTGPTGGDMLLNGKEPKRFYFIQCVGSRDRQSGARYCSRVCCMYTA
                     KHASIVKDRVKDAEVYVSYIDVRAYGKGYDEFYKTTQENGALYIRGIPGEVVEGDGCL
                     VVRVEDMLTGEMREIETDMVVLATGVRPRKATAELCDVLSIECDDYGFIKVDAVAPSQ
                     TNVDGIFVCGMGSGPKDVPDTVASGGEAASRCMEYIVKGTGS"
     gene            3149..>3426
                     /locus_tag="PKJ17_04585"
     CDS             3149..>3426
                     /locus_tag="PKJ17_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide reductase"
                     /protein_id="HOD75278.1"
                     /translation="MKTGIFICHCGHNIKHTVDVAGLSEYFRKYPQVTVSEDYPFLCS
                     EPGQNMITDSIEKHGLDRVIVASCAPSLHGELFKDLLRKKDVNPFLLRR"
BASE COUNT          791 a          870 c         1099 g          666 t
ORIGIN      
        1 aggtccgcgt gatcgatgcc gggaagcggg gggcacaggt aatagccgcc gcgtgtccct
       61 tctgtgtcat gacgctcgag gacccggcga ccgagaaagg attaattgta aaggagctgt
      121 cagaaatttt gatggaggtc atataatgaa tatccttgtg tttacgaaaa gggttgctgc
      181 aacccaggaa gaggagctga ggatccgcga cgagggtgcc tctgtcgatc tctccaaggt
      241 gccgttcaag atcaacgact gggataatta ttcgatcgaa gaggccgtga ggatcgtcga
      301 aaagacaggc ggaacggtga ccgcgatatc catcggcgac gccgaatcgg atgaggtctt
      361 aagaagggcc atcgcgatgg gtgcgaagga tggctttctc gtgaattccg agagcgtctt
      421 tcacgacccc ggggtgcgca gccgcctcat ccagaatttt atccagaagg agaacatacc
      481 attcgacgtc atcttcaccg gtgtccagtc ggaggatgac caattcggct gcgtcggcgg
      541 gatcctggga gctgtcctcg ggctgccgta ttcctcgatg gtgatcggga tagacgcagt
      601 cgagaaggac cacatgatca tccggaggga actcgaaggc ggactgcagg agaggataaa
      661 gattacggtc ccgtgcgtgc tgtcgataca gagcggtatc aacgagccgc ggtatgtctc
      721 gatcatgggc atccgcaagg cgagcaaggt cgaaagaaag gttttcaagg ccgcggatta
      781 cgcggctgaa gcaggacctg ggatcgaggt cgtgcagtgg gcgtacccgg agaagaaagg
      841 cgtcgcgacg atgcttaccg gcgaactcga tgtcgtatgc ggccagcttc tcggtatact
      901 caaggagaag ggggtgttcc aatgagttac gcattgataa tagcagaggt gcgacgcggc
      961 gtgttcgagg agaggaacct cgattcggcg ggcattgcgg cccttctcgg aaaagaagcg
     1021 gtcatgctgc tccctgacgg ggcgtaccag accgggaaca tcgcagatac gatagtgaag
     1081 gttaaggtcg gtgaagaggt gttcctgaac ccccttaacc tgaaaaacat actggagaag
     1141 gtttttacgg cccgcggcaa cccggatgta atcatattca cccattcctc gttcggcatg
     1201 gagtccgccg cgtacacggc gggcgccttc gggttcccgg tcgtcacgga tgtcagcgga
     1261 tatgacaagg aaagaggcgt ttttttgaaa agctattact ccgacaagat cttcggcgag
     1321 ttcaaggcga acggcgggcc tgtcgtcttg agcgtgcgga gcgggagctt caaggagttt
     1381 gccgcggaaa aggcagcggc cagagtcgag gaaatagagg gtgttgcccc tgtagagggc
     1441 cggaccttcg ttgaatacgt cgaggaagag aaagcggacg tcgacatcac gaaggcggat
     1501 ttccttctct ctgtcggcag gggcatcggg aacaaggatg agataccggc atacgaagac
     1561 ctcgccggca agctcaaggc cgtgctgtcc gggtcgcgcc cggtcattga caagatgtgg
     1621 cttccgaagc cgcggcaggt cggaacgtcg ggaaagaccg tcaagcccaa ggtatacctc
     1681 gccatgggta tatccggtgc attccagcat atcgccggaa tgaaggattc cgaatgcatc
     1741 attgcggtca acaaggacac ggaggccccg atcttccagt acgcgcattt cgggatcgtg
     1801 gccgacattc acaaggttcg tgacaaactg aaagagatca tgggttgatg gacaacagcg
     1861 gcaaaaatgt tctggtagtg ggcggaggga tcggcgggat taccgccgcc ctcgagctcg
     1921 catcgtgcgg catcaacgtg tcgatggtcg agtcgggccc gtcgatcggc ggaaggatga
     1981 tccagctcga caagacgttc ccgacgctcg actgctcgac gtgcaccctt tcgccgaaaa
     2041 tggtcgaggt ctccctaaat cacaacatcg acctcatgag cgtgtcgcag atcacgcaca
     2101 tggcgcggaa agggccgggg ttcctggtcg ggataaagaa aaacccgcgt tacgtcgacc
     2161 caaagaagtg cacggcatgc gggacgtgtt ccgaggcctg tgtcatgaaa gacaggatac
     2221 ctcacgagtt caatatgatg ctcgggaagc gggccgcgat atatatgccc tttccccagg
     2281 ccatacccca gaaatatctc gttgaccccg agcggtgcct gttcgtatct cgcgggaaat
     2341 gtaaaaaggc ctgcatggac gcgtgcgaaa cgggtgctat cgatttcgag gataaaggcg
     2401 aagaactgaa ggcgtacttc gacagcatcg tcctcgcccc gggcttcgat gtatatgacc
     2461 ctacggagaa acaggaactg ggctacggga cgctccccgg ggtcgtgacg ggcctcgaat
     2521 tcgaaaggat ctgctcggtg acggggccga ccggtggcga catgctgctc aacgggaaag
     2581 agccgaaacg cttttacttt atccagtgcg tcggctcgcg ggaccgtcag agcggcgcgc
     2641 gttattgctc cagggtttgc tgcatgtaca ccgcgaagca cgcctcgatc gtaaaggacc
     2701 gggtaaagga cgccgaggtc tatgtctcgt acatcgatgt cagggcgtac ggcaaggggt
     2761 acgacgagtt ctacaagaca acgcaggaga acggggccct gtacatccgg ggcatccccg
     2821 gagaggtcgt agaaggcgac gggtgcctcg tcgtacgggt tgaagacatg ctgacgggcg
     2881 agatgcggga gatcgagacg gacatggtcg tccttgcgac gggggtgcgg ccccgcaagg
     2941 caaccgcgga actgtgcgac gtcctttcca tcgagtgcga tgactacggg tttatcaagg
     3001 ttgatgccgt agccccgtca caaacgaacg ttgatggcat cttcgtatgc ggcatggggt
     3061 cgggcccgaa ggacgttccc gataccgtgg cctcgggagg tgaagctgca tcgcggtgta
     3121 tggaatatat tgtgaaggga accggttcat gaagacaggg atatttatct gccattgcgg
     3181 gcataatatc aagcacacgg tggacgttgc aggtttgagt gaatatttca ggaagtaccc
     3241 tcaggtcacc gtttccgagg actatccgtt cctgtgctcg gagcccgggc agaacatgat
     3301 cacggacagc atcgagaaac acggactcga ccgggtcatt gtcgcgtcgt gcgccccgtc
     3361 cctgcacggc gagctcttca aggacctcct gagaaagaag gacgtgaacc cgttccttct
     3421 gcgccg
//
LOCUS       DAOPOA010000168         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_30000_length_3423_cov_4.067399, whole genome shotgun sequence.
ACCESSION   DAOPOA010000168 DAOPOA010000000
VERSION     DAOPOA010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30000_length_3423_cov_4.067399"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKJ17_04590"
     CDS             complement(<1..502)
                     /locus_tag="PKJ17_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOD75279.1"
                     /translation="MRKGILAATTLAIFLVFSFGAAWSQTSGEAGRDTFRLTHIESFE
                     PFAVVENGTSKGLTVDILTEALARVGFKVVFAAQPQEKEQEMVLKGEADGLSFFAINA
                     ERKKSFDFSDPYLMTGGALFVKSPAPPCNSLKGLDGRTVATPLKGPLAGYIKKNFPEV
                     RVFTDVK"
     gene            793..>3423
                     /locus_tag="PKJ17_04595"
     CDS             793..>3423
                     /locus_tag="PKJ17_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOD75280.1"
                     /translation="MRALRCFITPLLAIILITAVFLSAASVCRSDDDVAVLARQIDDL
                     KKDGRVREAIPLAARLVSIRESTHGPDHPATAVALMGLGNLYRMAGLFTDAEPLLKRA
                     LSIQERAPRGPDRPAVAQNLMLLAIVYTSTGRYAEAEPLLKRALDIRQRTQMQRPGAI
                     AFNQVLLADVYHHVGREAEAESLLEDALNGWDKDQDLPPEAIAFSLTLIAKIYRTSGR
                     YAEAEPLIQRSLAICEKGLGPDHPTVAFALNNLAMIYRRTGRNADVESLYKRSLAIFR
                     KSFGDNHPAVSASLNNLAFYYGTTGGHQQAHSCFTSSLGIQDSLRESVFTLLTEKQKL
                     SYMKTQEMTIHGYVSHTLSFPRSSAATVVDAFNAWLRWKGSVIEAQGRYLDALGASDD
                     PKVRETFKELTALKRDIARLQLAGTGKTAPGDYARKMSELVKKKESVEPELMAASREF
                     MLQKKAQTADVGTLSSALSRDSAYLDYARITLFDFRKRTWTEPHYLLFLLTPGETVEV
                     QLIDTGASENVDRHINAYLGEMARAGREGVLPSEKKLKAEASVLYDILVKPVEPSLKG
                     RKRLLVSPDGNLNLIPFEALVEPAGTYLIEDFLVSYVGAGRDMLKFAKAKLAGGESLI
                     IADPDYDLGQKEVEKARSSAGLRSAGTKGHVSRDAAGMRFDRLPDTREEADTIEKILH
                     DRFGQKVFNEQDKRALELVLFRVSSPKVLHLATHGYFLAEEETKQSEKAAELPLTGDP
                     LQDTPITIENPMLRSGIVLAGANASLKEGKDEGMVTAEKVLGLNLGGTDLVVLSACQT
                     GVGDIQSGEGVFGLKRAFILSGAKTLVMSLWSVPSKETVDLMTAFYALLSEGKTPSEA
                     LSQAKLTLMKQKPHPFFWAAF"
BASE COUNT          729 a         1081 c          975 g          638 t
ORIGIN      
        1 ccttgacatc ggtgaacacc ctcacctcgg ggaaattctt cttgatgtag cccgccagag
       61 ggcccttcag cggggttgcc acggtcctgc catcgagtcc cttgagagaa ttgcagggcg
      121 gagcgggcga cttcacgaaa agggcccctc ccgtcatgag atagggatcg ctgaagtcaa
      181 aactcttctt tcgttcggcg ttgatcgcga agaaggagag gccgtccgcc tcccccttca
      241 gcaccatttc ctgctctttc tcctggggct gcgccgcgaa gacgaccttg aacccgaccc
      301 tcgccagcgc ctccgtaagg atgtccacgg tcaacccctt ggacgtgccg ttctcgacaa
      361 cggcaaaggg ctcgaaggac tcgatatggg tcagcctgaa agtatccctg cccgcctcac
      421 cgctcgtctg ggaccatgca gcgccgaagc tgaagacaag gaaaattgcc agagttgttg
      481 ccgccagaat acccttcctc atgaagctcc cccttttcga tcttattgtg ttcctttcga
      541 ccgcacttcg atcaggtgtc gacgatgcaa agggaccggc gatcctccac gcgatgaagg
      601 ctctcggacc ggaacgctcc gtcccattgt cactcttgtc gataacctta ttgtatccca
      661 tcgtggggga gttgttaaca atttgttcgt ttcgctgccc catgcggctt gaaaaggagg
      721 ggaggagatg ctagaatcga cgcgagcccc agaaactggc cctttctatc cgagatgatg
      781 gagatcagac ccgtgcgagc cctccgttgc tttatcaccc ctctcctggc catcattctt
      841 ataacggcag tgttcctctc cgcggcctcc gtctgccggt cggacgacga tgtcgctgtg
      901 ctcgcgaggc agatagacga tctcaaaaag gatggcaggg tccgggaggc tatccctctc
      961 gcggcgaggc ttgtttccat cagggagtcg acgcacggcc cggaccatcc cgccaccgcc
     1021 gtggcgctga tgggcctcgg caacctctac cggatggcag ggcttttcac ggacgcggag
     1081 ccgctcctca agcgcgctct cagcatacag gaaagggccc cccgcggccc ggaccggccg
     1141 gccgtcgccc agaacctcat gctcctcgcc atcgtctaca ccagcaccgg gcgatacgcc
     1201 gaggcagagc ccctcctcaa gcgcgctctc gacatccgcc aaaggacaca gatgcagcgc
     1261 cccggggcca ttgccttcaa ccaggtgctt ctcgccgatg tctaccacca cgttggccgt
     1321 gaggccgagg cggagtccct gttagaggat gcgcttaacg gctgggacaa ggaccaggac
     1381 cttcctcccg aggcgatcgc cttctccctc accctgatag caaagatcta ccgcacgagc
     1441 ggccgttacg ccgaggcgga accacttatc caacgctccc tcgcgatatg tgagaagggg
     1501 cttggcccgg accatcccac cgtcgccttc gccctcaaca acctcgccat gatctatcga
     1561 cgcaccggcc ggaatgccga tgtggaatcc ctgtacaagc gctccctcgc gatattccgg
     1621 aagagcttcg gggacaacca tcctgccgtc agcgcatcgc tcaacaatct tgccttctac
     1681 tacgggacga cgggcggaca tcagcaggcc cattcctgtt tcaccagctc ccttggcata
     1741 caggattcgc tccgggaatc cgtcttcaca cttctcacgg aaaagcaaaa actctcctat
     1801 atgaaaaccc aggagatgac gatccatgga tacgtgagcc acaccttgag ttttccccgc
     1861 agcagcgccg caaccgtagt cgatgccttc aatgcctggc ttaggtggaa gggctccgtc
     1921 atagaggccc aggggcgtta cctcgacgcc ctgggcgcgt ccgacgatcc caaggtgcgg
     1981 gagacgttca aggaactgac ggcgctgaag cgggatatcg caaggctcca gcttgccggg
     2041 acgggaaaga cggcgcccgg ggattacgcg aggaagatgt ccgagctggt gaaaaagaag
     2101 gaatctgtcg aacctgagct catggccgcg agcagggagt tcatgctgca aaagaaggcg
     2161 cagacggcgg atgtcgggac cctttcatca gccctttccc gggactctgc ctatctcgac
     2221 tacgcaagga tcaccctctt cgatttcagg aagagaacat ggacggaacc ccattacctg
     2281 ctcttcctcc tcaccccggg ggagaccgtg gaggtacagc tcatcgacac gggggcgagc
     2341 gaaaacgtgg acaggcacat aaacgcgtac ctcggggaga tggcacgtgc cggaagggaa
     2401 ggcgtgctgc cgagcgaaaa gaagcttaaa gccgaggcat cggtcctcta tgacattctc
     2461 gtcaagcccg tcgaaccttc cctgaaaggg agaaagcgcc ttctcgttag tcccgacggc
     2521 aatctcaacc tcatcccctt tgaggccctt gtggagccgg caggcaccta tctcatcgaa
     2581 gatttcctgg tcagttacgt tggagcgggc agagacatgc tcaaattcgc gaaggcaaag
     2641 cttgcaggcg gtgaatcgct cataatagca gaccccgact acgatctggg acagaaggag
     2701 gtcgagaagg cgcggtcatc ggccggtctc aggtcggcgg gcacgaaagg ccatgtctcg
     2761 agggacgcgg caggcatgcg tttcgaccgt cttcccgaca ccagggaaga ggccgacacc
     2821 attgaaaaga tcctgcatga caggttcggg cagaaggtgt tcaacgaaca ggacaagagg
     2881 gcgctggagc ttgtcctgtt ccgcgtgtcc tccccaaagg tccttcacct ggccacgcac
     2941 gggtacttcc tcgccgaaga ggagacaaag cagtccgaga aggcggcaga gctcccgttg
     3001 acgggcgacc cgctgcagga cactccaatt accatagaga acccgatgct gcgctcgggt
     3061 atagtccttg ccggggccaa cgcgtctctc aaggagggaa aggacgaggg catggtcacg
     3121 gccgagaagg tcctcgggct caacctcggg ggcaccgacc tcgtcgtcct gtccgcctgc
     3181 cagacaggcg tcggcgacat ccagagcggc gaaggcgtct tcgggttgaa aagggccttc
     3241 atcctttccg gggcaaagac cctcgtcatg agtctctgga gtgttccctc aaaagaaacg
     3301 gtggacctga tgaccgcctt ctacgccctc ctttccgagg ggaagacccc gtcagaagcg
     3361 ctcagccagg caaagctcac cctcatgaag caaaagcccc accccttctt ctgggcagcc
     3421 ttc
//
LOCUS       DAOPOA010000169         3415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_30084_length_3415_cov_2.517262, whole genome shotgun sequence.
ACCESSION   DAOPOA010000169 DAOPOA010000000
VERSION     DAOPOA010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30084_length_3415_cov_2.517262"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKJ17_04600"
     CDS             <1..462
                     /locus_tag="PKJ17_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75281.1"
                     /translation="HNNQLVGRTDRSGRLFIPGLTSYVDNHISIDDRDIPVEYSLKEV
                     GKYVSPPFRSGALLAFDAVRTRAVTGRMETGPRGKPVPVALGQAAFTLSGKEVSFITG
                     RKGEFYLENIPAGRYTGTVRDARRACSFTLVVSPSDDAITNLGGILAENCH"
     gene            443..931
                     /locus_tag="PKJ17_04605"
     CDS             443..931
                     /locus_tag="PKJ17_04605"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HOD75282.1"
                     /translation="MLKTAIDPKTTPARPLRLALYLCLLLLPLAGHAQGIDNCDVISA
                     RITCPEYDAFNTGETYVDGWITVACGTAATVTIAIGPGASGDVVNRTMTMTGANPLKY
                     NLYTDPARNQLWGDGTSGATVTVTVSANTEVHVPVYGRIPDRQQIASGGYSDSPVITL
                     TW"
     gene            1000..1695
                     /locus_tag="PKJ17_04610"
     CDS             1000..1695
                     /locus_tag="PKJ17_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOD75283.1"
                     /translation="MFAILGGTSLLGSRIFSGWPDETVETPFGTVTVKKGDGAVFLQR
                     HGSPPMPPHRIRHRANIAALRELGVRRIVAVNSVGSLTFSLEPGTLLVPDDFVSPWHV
                     ETFFDDEMRFMIPVMDGNFAHEVFVACREHTMAVRMGGTYVQTIGPRLETKAEINILK
                     GFGDIVGMTLASEATLAMEMDLPYASICSIDNYCHGIVEVPLTMDEIHANMSRNLATI
                     ESVLNTLITRGLS"
     gene            1692..2948
                     /locus_tag="PKJ17_04615"
     CDS             1692..2948
                     /locus_tag="PKJ17_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOD75284.1"
                     /translation="MTILIKGVLLDGAVRDIFIDNGVILEISPSCTRPADRTIDGTGK
                     AALPSLINGHTHAAMTLFRGYADDMPLKSWLEEKIWVLESKLTEEDVYRGARLACLEM
                     IKNGVTAFNDMYWHWEATARAAADMKVRGFISAVFIDMFDDARSREQIALNHDLYTLS
                     KKYEPYATFTFGPHALYTVSKASLEWMRDFSVDKDILIHMHLAETADETRFSHEKYGM
                     SPVEFLDSIGLLSRRYIGCHGCALDEADVRVLSERGARLVHVPVSNLKLAVGRVFPHR
                     LIGDAGIPYCFGTDGCASNNHLDVLETMKFASLLAKFHTSDPTMLPARQTFDMATRTA
                     ADIFRLGEWSIREGLRPDIILIDLARPEFVPNFDIYSDMVYAANGSAVDTVICMGEVL
                     MEGRVVPGEEEIMAKAAETARALVTR"
     gene            2945..3400
                     /locus_tag="PKJ17_04620"
     CDS             2945..3400
                     /locus_tag="PKJ17_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOD75285.1"
                     /translation="MIFVCDVMLGKLARYLRTLGFDARYVRQGEDPASFPAPSCQCVF
                     LTRRHDRTGRPHTVVIRDNDPAKQILEIKEIIDRHVDPKAFMSRCLECNIPLIDTPKE
                     EIEPFVPEYIYHHHDRFKTCPSCKKVYWEGSHAEEMREWLEKLIDGAAR"
BASE COUNT          718 a         1130 c          961 g          606 t
ORIGIN      
        1 cacaacaacc agctggtggg ccgcacggac cgttccggcc gcctcttcat accgggcctg
       61 acctcctacg tggacaacca catctccatc gacgacaggg acataccggt ggagtactcc
      121 ctgaaggagg tgggaaaata tgtctcgccc ccattcagaa gcggcgcact cctggccttc
      181 gacgccgtga gaacccgggc cgtcaccggc cggatggaaa caggaccgcg agggaaaccc
      241 gttcccgtgg ccctcgggca ggccgcattc acgctgagcg gaaaagaggt ctctttcatc
      301 accggcagga agggtgagtt ctacctcgag aacatccccg ccggccgata caccggcacg
      361 gtccgggacg ctcgccgggc atgctcattc accctcgtcg tttcgccctc cgacgacgcg
      421 atcacaaacc tgggaggtat tcttgctgaa aactgccatt gacccgaaga ccacaccggc
      481 gagacccttg aggcttgccc tttacctgtg ccttctcttg ctacccctcg ccgggcacgc
      541 ccagggcatc gataactgtg atgtgatctc ggcccgcatc acctgccccg agtacgacgc
      601 cttcaatacc ggcgaaacct atgttgacgg atggataacc gtggcatgcg gcacagccgc
      661 aacggtgacc atcgccatcg gccccggtgc atcgggggat gtggtcaaca gaacgatgac
      721 gatgacagga gcgaaccccc tgaaatacaa cctttacacg gacccggcgc gtaaccagct
      781 gtggggtgac ggcaccagcg gcgcaacggt cacggtcact gtgagcgcga acacggaggt
      841 gcacgtcccc gtctacggta gaataccgga ccgtcagcag atcgcgagcg gcggatacag
      901 cgactcgccc gtcatcaccc tgacatggtg agcgggagcg acccccaaca attctattga
      961 ctcaacgcgc cattccctgt agaagaatat ctggaaacca tgttcgcgat actcggagga
     1021 acatcactgc ttggctcccg gatcttctcc ggctggcccg atgaaacggt agaaacccct
     1081 ttcggcaccg tcaccgtgaa gaaaggggac ggggcggttt tcctccagag acacggcagc
     1141 cccccgatgc cccctcacag gatacggcac cgggcgaaca tcgcggccct cagggaactg
     1201 ggggtgcgca ggatagtggc cgtcaactcc gtggggagcc tcacgttctc ccttgagccc
     1261 ggcaccctcc tcgttcccga cgatttcgtc tccccctggc acgtcgagac cttcttcgac
     1321 gatgagatgc gtttcatgat acccgtaatg gacgggaact tcgcccacga ggtgttcgtc
     1381 gcgtgcaggg agcacacaat ggcggtgcgg atgggcggca cctacgttca gaccatcggg
     1441 ccgaggctgg agacgaaggc cgagatcaac atactcaagg gcttcggaga tatcgtcggg
     1501 atgaccctcg cctccgaggc gaccctcgcc atggagatgg acctgcccta cgccagcatc
     1561 tgttccatcg ataattattg ccacggcata gtcgaggtgc ccctgaccat ggacgagatc
     1621 catgcgaata tgtcacggaa cctcgcaacc atcgagagcg tcctcaacac cctcatcacg
     1681 cggggcctct cgtgacgatc ctcatcaagg gtgtcctcct cgacggcgcc gtcagggaca
     1741 tcttcatcga caacggcgtc atcctcgaga tctccccgtc atgcacccgt cctgccgaca
     1801 gaacgataga cggcacgggc aaggccgccc tgccctccct catcaacggg cacacccatg
     1861 ccgccatgac cctcttccgg gggtatgccg acgatatgcc cctgaaatcc tggctcgagg
     1921 agaagatatg ggtcctcgaa tccaagctca ccgaagaaga tgtataccgg ggggcgcgcc
     1981 tggcctgcct ggagatgatc aagaacggcg tcaccgcctt caacgacatg tactggcact
     2041 gggaggcaac cgcccgggcg gccgccgata tgaaggtgcg gggtttcatc agcgccgtct
     2101 tcatcgacat gttcgatgat gccaggtccc gggagcagat agcactcaac cacgacctct
     2161 acacgctctc gaagaagtat gaaccctacg caacctttac ctttggcccc cacgcgctgt
     2221 acacggtatc gaaggcgagc ctcgaatgga tgcgcgactt ctccgtcgac aaggacatcc
     2281 tcatccacat gcacctggcg gagaccgccg acgagacgcg cttctcacat gagaagtatg
     2341 gaatgtcccc cgtggagttc cttgactcca tcggtcttct ttccaggcgc tacatcggct
     2401 gccacggctg cgccctcgac gaggctgacg tccgcgtgct cagcgagcgc ggcgcccggc
     2461 ttgtgcacgt gcccgtctcc aatctgaagc tcgccgttgg acgggtcttc cctcaccgtc
     2521 tcatcggcga cgcggggata ccctactgtt tcgggaccga cggatgcgcc tccaacaacc
     2581 atctcgacgt tctggaaacg atgaagttcg cctccctgct cgccaaattc cacacatcgg
     2641 acccgacgat gctgccggcc cggcaaacat tcgacatggc caccaggacc gccgcggaca
     2701 tcttccgcct cggcgagtgg tcgatcaggg aaggcttacg tcccgacatc atccttatcg
     2761 acctcgcgag gcctgagttc gtccccaatt tcgacatcta ttcagatatg gtctacgccg
     2821 cgaacggctc cgccgtcgac acggtcatct gcatggggga ggtcctcatg gagggacgtg
     2881 tcgttcccgg cgaagaggag ataatggcaa aggccgccga gacggcccgc gccctcgtca
     2941 cgagatgata ttcgtctgcg acgtcatgct gggaaagctt gcccggtact tgaggaccct
     3001 gggtttcgac gcccgctatg tgcgccaggg agaagacccg gcgtcctttc ccgcgccttc
     3061 ctgccagtgc gtcttcctca caaggaggca cgacaggacg ggtcgtcccc acacggtcgt
     3121 catccgtgac aacgaccccg cgaagcagat cctcgagata aaagagatca tagacagaca
     3181 tgtcgacccg aaggcattca tgtcgcgctg cctcgaatgc aacataccgc tcatcgacac
     3241 gcccaaagag gagatcgagc ccttcgtccc ggaatacata taccaccacc acgaccggtt
     3301 caagacctgc ccgtcgtgca agaaggtcta ctgggagggc tcacacgcgg aagaaatgcg
     3361 cgagtggctc gagaagctca tcgacggtgc cgcccggtga gccgacggac ccatg
//
LOCUS       DAOPOA010000170         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_30211_length_3404_cov_3.227232, whole genome shotgun sequence.
ACCESSION   DAOPOA010000170 DAOPOA010000000
VERSION     DAOPOA010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30211_length_3404_cov_3.227232"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PKJ17_04625"
     CDS             <1..1272
                     /locus_tag="PKJ17_04625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOD75286.1"
                     /translation="ATEYLLFIPRTIPMLAGMATLERFIRAARSTGAGTVYSDFHDDT
                     GKGKTLHPLIDYQPGSVRDDFNFGALALLDASIVRDTLQRYGRTPPLRHGALYDIRLK
                     MSIDHGIHHIAEPLYSVVVHGDTPGADAHFAYVDPRNQALQREMETVFTDHLKRINAY
                     LPPDRLKTMEEPAEPFPVRASVIIPVKNRRKTIADALQSALSQETDFTFNILVVDNHS
                     TDGTTEVIADMAGRNPAIRHIIPRRKDLGIGGCWNAAIMDDACGRYAVQLDSDDLYEN
                     HLVLETITDIMRDGRYAMVVGSYTIVNERLEQIPPGLIDHREWTADNGHNNALRVNGL
                     GAPRAFSTTAIRRIGFLNVSYGEDYAAALRMSREYRIGRIYESLYLCRRWQGNTDAGL
                     SLEAANRNDAFKDSLRTAELVERQKLAGESP"
     gene            1273..2250
                     /locus_tag="PKJ17_04630"
     CDS             1273..2250
                     /locus_tag="PKJ17_04630"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOD75287.1"
                     /translation="MTRALRDRTYATFEGGKSASSLEGLCKGLLAEQRASWPELRQAY
                     DSLRGTRYRDMACNGFSVRLLFNPGRAVNTTAAVSPEEIRERPCFLCVANLPAGQRYI
                     LYRREFLILGNPRPVVPFHLTIAHLDHQPQAIAGHMEAFLRIAADLGPGFTTLYNGPR
                     CGASAPDHLHFQAVPSGQMPIEKEIDGTKGSTLPSLTAGVSVFRMAGLGREIILIEGG
                     DARPVADAFEHYVRALKDGLAPPGQPGEEPMLNAAASFDGKTWRLLVFPRRAHRPAAF
                     YRKDDGKVLVSPAIMEMAGVIVTPAERDFDRLDAGAIESLYREVSLSHD"
     gene            2411..>3404
                     /locus_tag="PKJ17_04635"
     CDS             2411..>3404
                     /locus_tag="PKJ17_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOD75288.1"
                     /translation="MQELTREISLLKDKIRELEKSEAHHKKVVEELGSSLQQLRLLVD
                     AGPDFFFFKDLDLTYRLVNLANAKFFGLTEAEIIGKNDYDLMPGEAAVACQQSDRLAI
                     TEKRTIITTEPVGGRFYETYKFPFLVRGEVAGVAGIVRDITERKLAEEELRKSRALLS
                     DMIENSGALICVKDRDGRYEMVNRKWQEVTGISRENTIGRTDRELFPGLTGIVFRRND
                     LEVMESGSVMEKEEVLESDQGNRFFISIKFPLRDQDGSVSGMCAMITEITARKSVEEQ
                     LAISRDRLSRAEIISRSGNWEFDMETKEVFMSPGARRIFGAGDGVLTIPAMKEIP"
BASE COUNT          740 a         1073 c          995 g          596 t
ORIGIN      
        1 gcgacggagt atcttctgtt catcccccgg accatcccga tgctcgccgg catggcaaca
       61 ctggagaggt ttatccgcgc ggcccggtcc acgggagccg gcaccgtcta ttccgacttc
      121 catgacgaca cgggaaaggg gaagacactc catcccctca tcgattatca gcccggcagc
      181 gtgcgggatg atttcaattt cggcgccctc gcccttcttg acgcctcgat cgtccgcgac
      241 acgcttcaaa ggtacggacg cacaccgccc ctgcgccacg gggccctgta cgatattcgg
      301 ctgaagatgt ccatagacca tgggatccac catatcgcgg aaccgctcta cagtgtcgtg
      361 gtccacggtg acactcccgg ggcagatgca cacttcgcat acgtcgaccc ccgcaaccag
      421 gcccttcaga gggaaatgga gacggtcttc accgatcacc tcaagaggat caacgcgtac
      481 cttcctccgg acaggctcaa gacaatggaa gaaccggcgg agccctttcc cgtgagagcc
      541 tccgtgataa tccccgtgaa gaaccgtaga aagaccatag cggacgccct ccagagcgcc
      601 ctctcacagg aaacggactt caccttcaac atccttgtcg tcgacaatca ttcgacggac
      661 gggaccacgg aggtcatcgc cgacatggca ggcaggaacc cggccatacg gcacatcatc
      721 ccccgccgga aagacctggg gatagggggg tgctggaacg cggccatcat ggacgacgcc
      781 tgcggccgct atgccgtcca gctcgactcc gacgacctgt acgagaacca ccttgttctc
      841 gagaccatca cggatatcat gcgcgacggg agatacgcaa tggtcgtcgg atcctatacc
      901 atcgtcaacg agcggctgga gcagatacct cccggactga tagaccatcg tgaatggacg
      961 gccgacaacg gacacaacaa cgccctcaga gtcaacggcc tcggggctcc gcgggcattc
     1021 tccacgacgg ccatccggcg gataggtttc ctcaacgtga gctacggaga ggactatgcc
     1081 gccgcgctga ggatgtcccg ggaatacagg atcggaagga tctatgaaag tctctacctg
     1141 tgccgccgct ggcagggcaa caccgatgcc gggctttccc tcgaagcggc aaacaggaac
     1201 gacgccttca aggactccct ccgaaccgcc gagctcgtcg agcggcagaa gctggcaggg
     1261 gagtccccct gagtgacacg cgcgttgaga gaccggacct acgcgacctt cgaaggggga
     1321 aaaagcgctt cctcactcga gggattgtgc aaggggcttc ttgccgaaca gcgggcatcc
     1381 tggccggagc tccggcaggc ctatgattcc cttcgcggga cgcggtaccg ggacatggcg
     1441 tgcaacggat tctcggtccg tctcctgttc aatcccggca gggccgtcaa cacaacggcc
     1501 gccgtaagcc ccgaagagat cagggaacga ccctgcttcc tctgcgtcgc caacctgccc
     1561 gcaggacaga gatacatcct ctaccggcgc gagttcctga tcctgggaaa cccccggccc
     1621 gtggtaccct ttcacctcac catcgctcac ctcgatcatc aaccccaggc cattgccggt
     1681 cacatggagg cctttctgcg catagcggcc gacctcggtc ccggttttac aaccctgtac
     1741 aacgggcctc gctgcggggc ttcagcgccc gaccatctcc atttccaggc ggtaccctcc
     1801 gggcagatgc ccatagaaaa ggagatcgac gggacgaagg gctccactct cccgtcgctg
     1861 actgccggcg tatccgtgtt caggatggcc ggcctcgggc gcgagatcat cctcatcgaa
     1921 gggggcgacg cccggcctgt ggcagacgcc ttcgagcact acgtgcgcgc cctcaaagac
     1981 gggttggcac cccctggtca gccgggcgag gaacccatgc tgaacgctgc tgcttctttt
     2041 gatggaaaga cgtggcgcct tctcgttttt ccccgccgcg cccaccgccc ggccgccttc
     2101 tacaggaagg atgacgggaa agtcctcgtc agcccggcga tcatggaaat ggcgggcgtc
     2161 atcgtcaccc ccgcggagcg tgatttcgac cgcttggacg ccggggcgat agagtccctg
     2221 tatcgcgagg tctctctcag ccacgactga acacggccta aacctctgat cccgtagtgg
     2281 cgataagata tctggaggtc agcacgatac caggcgcggc actcaaggaa caccgccgcg
     2341 gattccatat tgatataccc gcgtcatatc tattattatt gttcaaggga gaggacaaac
     2401 gttgaagacg atgcaggaat tgacccggga gatctccctc ttaaaggaca agatccggga
     2461 actggagaag tccgaggcac accacaagaa ggtcgtggag gaactggggt catccctgca
     2521 gcagttgcgc ctcctcgtcg atgcgggacc ggacttcttc ttcttcaagg atctcgacct
     2581 cacctaccgg cttgtcaacc tggcaaacgc gaagttcttc gggctcaccg aggccgagat
     2641 cataggcaag aacgattacg atctcatgcc tggcgaagca gcggtcgcct gccagcagag
     2701 cgatcggttg gccatcaccg aaaaaaggac gatcatcacc accgaacccg tcgggggcag
     2761 gttctatgag acctacaagt tccccttcct cgtcaggggc gaggtggcag gtgtcgcggg
     2821 gatcgtccgc gacatcacgg agcgcaagct cgccgaggag gaactgcgca aaagccgtgc
     2881 cctcctgtcg gacatgatcg agaacagcgg cgccctcatc tgcgtcaagg accgtgacgg
     2941 ccgttatgaa atggtgaacc gcaagtggca ggaagtgacc ggcatcagcc gggaaaatac
     3001 catcggcaga acggaccggg aactctttcc cggactgacg gggattgttt ttcgccggaa
     3061 tgacctggag gtcatggaat caggatcggt gatggagaag gaggaggtcc tggaaagcga
     3121 ccagggaaac cggttcttta tctccatcaa gttccccctg cgcgatcagg atggttccgt
     3181 gagcggcatg tgcgccatga tcaccgagat cacggcacgc aaatccgtgg aggaacagct
     3241 tgccatcagc agagaccgcc tgtcccgggc ggagatcata tcccgctccg gcaactggga
     3301 gttcgacatg gagacgaagg aagtcttcat gtccccgggg gcccgcagga tcttcggtgc
     3361 cggagatgga gtattgacga tcccggccat gaaggaaata cccc
//
LOCUS       DAOPOA010000171         3391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_30367_length_3391_cov_3.381894, whole genome shotgun sequence.
ACCESSION   DAOPOA010000171 DAOPOA010000000
VERSION     DAOPOA010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30367_length_3391_cov_3.381894"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PKJ17_04640"
     CDS             <1..441
                     /locus_tag="PKJ17_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOD75289.1"
                     /translation="GDPLLFRVLQPLLVGDIPADHDLVLHPFAYAGWVGLFVTSLNLL
                     PVGQLDGGHVIYAIFGEKSTWVYRAVILGLGVLAALTNIGWLTLVILLLIFGMRHPRP
                     FDDETELDRRRKKLAIAVLAIFILSFIPSPFPEMNLTKIIEKGF"
     gene            complement(554..1174)
                     /locus_tag="PKJ17_04645"
     CDS             complement(554..1174)
                     /locus_tag="PKJ17_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75290.1"
                     /translation="MRNIIITAVFLAFILCSCTPGPVYVAKGESDSVKRSYNDRHVQS
                     IFDKNAGLFKDIYNRYSSDNVGIYQDGIGITTLEDFDKQQLHYLMVYVRPKEASFDGN
                     STKPEERIAKIIRDFVPKYVKRVRPADLDGDHIEGLAFGVYWAARDYSQCDTYGGHIE
                     YLYIFFPEDAAKAYLEGRLSLVEAIEQSEVMSSTELKPAQPIRPVF"
     gene            complement(1171..1497)
                     /locus_tag="PKJ17_04650"
     CDS             complement(1171..1497)
                     /locus_tag="PKJ17_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOD75291.1"
                     /translation="MNGREIPDRVFYRIKEVCTLTGLKPHVLRYWEQEFKDIKPVKSP
                     TGQRLYKKKDLEIIFTIKKLLHEKRFTIDGAKQYLANHKRILNEIREELTELVAMLKQ
                     EEKEKK"
     gene            complement(1494..1784)
                     /locus_tag="PKJ17_04655"
     CDS             complement(1494..1784)
                     /locus_tag="PKJ17_04655"
                     /inference="COORDINATES: protein motif:HMM:NF001401.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HOD75292.1"
                     /translation="MTKIDIVTDLYEKLGFSKRECAHIVDRFFEIIKDTLATDENVKI
                     SGFGNFFVRKKKARRGRNPQTGEEIKITERKVLSFRLSQVLKDEINDSRRHE"
     gene            complement(1794..>3391)
                     /gene="pheT"
                     /locus_tag="PKJ17_04660"
     CDS             complement(1794..>3391)
                     /gene="pheT"
                     /locus_tag="PKJ17_04660"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOD75293.1"
                     /translation="GQPLHAFDSERLDESRIDVKVAGGPSTFRTLDSIDRPIEAGDIL
                     ICDGSGPVAVAGIMGGENSEITGTTRNMALEAAYFNPYSIRATARRLGIRSEASLRFE
                     KGIDIDNVGYAAERAMYLMHKLGGGQVVRGVREIFEKKEPRTIYITYSNINGLLGTHI
                     SRDAIARALKSIDLLIMKEDENGFVVSVPNFRHDIGEAADVIEEISRVHGFEHIPDTS
                     PVTVLKPQKVTRKERFLSMIKEYCLGTGFYEIVNFSFFSTKDIENFRIPEDDERARYV
                     SIMNPISRDWGVMRTMMTPSVLRCMAYNINRGTKNLRFFEKGKVFFQEDERIREHVAL
                     CFALTGREKEYSWKEKHAEYDFFDIKGVIEGLMERLGASFEVVPTTEPFLDRGRSADI
                     MVDGAKIGWAGAVRDDVLGLYEIEQTIYCAELRFDIILEKGNLTLQYKPIPRYPQVTR
                     DFSFFVDDRVPIATLVEKIKGISPLIVSVGIFDMFRKEMRSVAIRVVFQSHEDTLTDE
                     RVNALQERIIGELTNIDGISLRA"
BASE COUNT          637 a          982 c          899 g          873 t
ORIGIN      
        1 ggggaccccc tccttttcag ggtgcttcag ccacttctcg ttggcgatat ccccgctgac
       61 cacgatctcg tcctccatcc ttttgcctac gcaggctggg tggggctttt cgtcacgtcc
      121 ctcaacctcc tgcccgtcgg ccagcttgat ggaggccacg tcatctatgc catcttcggg
      181 gaaaagagca catgggtcta ccgcgccgtc atcctgggcc tcggggtcct cgcggccctt
      241 acgaacatcg gctggcttac cctggtgatc ctcctcctca tcttcggcat gcgccacccc
      301 aggccgttcg acgacgaaac tgagctcgac aggcggcgca agaagctggc gatcgcggtc
      361 ctcgccatct tcatcctctc cttcatcccc tctcccttcc ccgaaatgaa cctcacaaag
      421 atcatagaaa aagggttcta ggccgttctt cgccgcgtcc gcccgttagg cgctttggcg
      481 ccgacccgtg tgcccgtcca cgcaataaaa aagccgcagg gtaacctgcg gcttttttat
      541 tgcggttctc tctctaaaag accggccgta tcggttgtgc cggtttcagt tccgtgctcg
      601 acatgacctc ggactgctcg atcgcctcga cgaggctgag cctgccttca agataggcct
      661 ttgcggcgtc ttcggggaag aagatgtaga gatactcaat atgtccacca tatgtgtcgc
      721 actgggaata gtccctggcc gcccagtaaa caccgaacgc gaggccttcg atgtggtctc
      781 cgtcgaggtc ggcgggcctg accctcttga cgtatttggg cacgaagtcg cggattatct
      841 ttgcgatgcg ctcctcgggt ttcgtcgaat tgccgtcaaa ggaggcttcc ttgggcctca
      901 cgtagaccat gaggtagtgc aactgctgct tgtcgaagtc ttcaagggtc gtgatgccta
      961 tgccgtcctg gtatatcccc acgttgtcgg agctgtagcg gttgtaaatg tccttgaaga
     1021 ggccggcgtt cttgtcaaag atggactgga cgtgtctgtc gttgtagctt ctcttcacgc
     1081 tgtctgattc gcccttggcc acgtagaccg gccccggcgt gcatgaacac aggatgaagg
     1141 ccaaaaacac tgcggtgatg atgatgtttc tcatttcttt tctttctcct cttgtttaag
     1201 catagcgacg agttcggtaa gttcttcccg aatctcattg agtattcgtt tgtgatttgc
     1261 gagatactgc tttgccccgt caatggtgaa tcgcttctcg tggaggagct ttttgatggt
     1321 gaagatgatc tcgaggtctt tcttcttgta gaggcgctgc ccggtgggac tctttacggg
     1381 cttgatgtcc ttgaattcct gctcccagta gcgcaggacg tgtggcttga gtcccgtcag
     1441 cgtgcagact tccttgatac ggtaaaagac cctgtcgggt atttctctgc cgttcattcg
     1501 tgccttctac tgtcgttgat ttcgtccttt aatacctggc tcagcctgaa actcagcacc
     1561 ttgcgctctg ttatcttgat ctcttcgccg gtctgcgggt tccgtccgcg gcgggccttc
     1621 ttctttctga cgaagaaatt gccgaaaccg gaaatcttta cgttctcatc ggtggcgaga
     1681 gtgtccttta tgatctcgaa gaaccggtcg acgatatgcg cgcactcccg tttggaaaat
     1741 ccgagcttct cgtaaagatc ggttacaata tctattttcg tcataactcc tccttatgcc
     1801 cttaacgaaa ttccgtcaat attggtcagc tcgccgatga ttctttcctg cagggcattg
     1861 acacgttcgt cggtaagagt gtcttcgtgg gattggaaca ccacgcgaat ggctacactt
     1921 cgcatctcct tccggaacat gtcaaatatc cccaccgaaa cgataagggg tgagataccc
     1981 ttgatcttct caacaagcgt ggctatgggc acgcggtcat ccacaaagaa cgagaaatcc
     2041 cgtgtaacct gcggataacg gggtatcggc ttgtattgaa gtgtcaagtt ccctttttct
     2101 aagattatat caaatcgtaa ctcagcgcaa taaatagttt gttcgatctc gtagagtcca
     2161 agaacgtcgt cacggaccgc tccggcccaa ccgatttttg ctccgtcgac catgatgtcg
     2221 gcgctccgac cgcggtcgag aaaaggttcg gtggtcggga cgacctcaaa gctcgccccg
     2281 agcctctcca tcaaaccctc gatcactccc ttgatgtcga agaaatcata ctctgcatgc
     2341 ttttccttcc aggaatactc cttttccctg cccgtcaggg cgaagcagag ggcgacgtgc
     2401 tcacggatgc gctcgtcctc ctggaagaag acctttcctt tctcgaagaa cctcaggttc
     2461 ttcgtgcccc ggttgatatt gtacgccatg cacctcagga cggagggcgt catcatggtc
     2521 ctcatgaccc cccagtcgcg ggagatgggg ttcatgatgg acacgtacct cgcgcgttcg
     2581 tcatcctcgg gaatacggaa attctcgatg tccttcgtgg agaagaagga gaagttgacg
     2641 atctcgtaga agcccgttcc caggcaatac tcctttatca tcgacagaaa gcgctctttt
     2701 ctggtgacct tctggggctt gaggaccgtc acggggctgg tgtcgggaat gtgttcgaac
     2761 ccgtggaccc gggatatctc ctcgatgaca tcggccgcct cgcctatgtc atgcctgaag
     2821 ttgggcacgg acacgacgaa cccgttctcg tcctctttca tgataagaag atcgatggat
     2881 ttgagggccc gcgcgatggc gtcccggctg atgtgggtgc ccaggagtcc gttgatgttg
     2941 ctgtatgtga tgtagatcgt ccggggctcc ttcttctcga agatctctct gactcccctc
     3001 accacctggc cgccgccaag cttgtgcatg aggtacatcg ccctctcggc ggcatacccg
     3061 acattgtcga tatcgatgcc cttctcgaac ctgagcgacg cctcggacct gatcccaagc
     3121 cttctggcgg tggcccgtat ggagtaggga ttgaagtagg cagcctcaag ggccatgttc
     3181 ctcgtcgtgc ccgtgatctc cgagttctcg ccgcccatga tgcccgcgac cgccacgggt
     3241 ccggagccgt cacagatgag gatgtccccg gcctctatgg gcctgtcgat actgtcaagg
     3301 gtacggaatg tcgatggtcc gccggccact ttcacgtcta tcctcgactc gtcgaggcgc
     3361 tcggagtcga aggcgtggag cggttgtccg a
//
LOCUS       DAOPOA010000172         3374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_30568_length_3374_cov_3.353420, whole genome shotgun sequence.
ACCESSION   DAOPOA010000172 DAOPOA010000000
VERSION     DAOPOA010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30568_length_3374_cov_3.353420"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PKJ17_04665"
     CDS             <1..1026
                     /locus_tag="PKJ17_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD75294.1"
                     /translation="EPGFETPGTIRQEAEKALAEGKTFYTHSLGIPELRQAVSRHYRD
                     RYDLDISPERVIITNGSSGAFILLFAALLERGRTLDIADPGYPCYRNIALLTDCAVRA
                     LPVSAETNYEVLPEQLGGSPAPDVLVIANPSNPTGTIYRAGALNAIHKRLSSRGGVLV
                     VDEIYSGLVYDAPFSSSAAISEDVIVVNGFSKAFAMTGWRLGWMVVPPGLVRPIQKLA
                     QNLFIAPPSISQYAALAAFDDADGLARMCGLYRERRDFLLPRLRGLGFDIPVTPDGAF
                     YIYAGIERFGMDSMLFVERALAEAKVAITPGYDFGRFGAASHVRFSYADRLENLVEGC
                     DRLERWL"
     gene            1110..1406
                     /locus_tag="PKJ17_04670"
     CDS             1110..1406
                     /locus_tag="PKJ17_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75295.1"
                     /translation="MPKIAPGSLEPGMKTAKPVVNTSGMVLLGENTELTPELIDRIRN
                     MEVDGVWIQGLSKPSVPMEEVVAELDRRFRLVGGQPHMDIIRKAVQRHIEGLYE"
     gene            1399..2235
                     /locus_tag="PKJ17_04675"
     CDS             1399..2235
                     /locus_tag="PKJ17_04675"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOD75296.1"
                     /translation="MNSMDVRAVREKIEHINALPTIPKVLNRLLALIENPRVSLNEIS
                     NFISSDPALTTKVLRMVNSPVYGFPGRISSVNQAVILLGLTVVKGLLLGVSVFELMQK
                     TMIGLWEHSIGTALFSRIIAVRAGHKEPDEVSVDGLLHDIGKVFLVLQFPSEYQRALT
                     QTKEDGIIIHEAEKNHFSTTHASVGSWMARKWRFPAKLIDVIEYHHKPHLTKSTAVES
                     AIVHIADILVRARGFGFPGDDLMTPVQPEAWDLLKLTERDILEILEEAEDSLQATEDL
                     TL"
     gene            2232..3167
                     /locus_tag="PKJ17_04680"
     CDS             2232..3167
                     /locus_tag="PKJ17_04680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOD75297.1"
                     /translation="MNEKKTVVVISQDVVLSGIIDRILSRDYGVFLFRNIQSAIDYIY
                     NSPPNLIILDTSEEDIYSMDVINNLKSDPIFYQLPVLVVIDDIPSVPVWKDLFVEDYL
                     KREDIEQEGVTRVALAILRSERIVEVNPLTKLPGNISISRQIQARIEAGDVFALAYAD
                     LDHFKPFNDHYGFTRGDEVIRITGRLILNIVKSKEPQTAFVGHIGGDDFVFITSVDVV
                     EEASADIIDAFDRITPSLYDRKDRDAGFIETLDRQGNTRKFPVIALSIGIATNRTRSF
                     THFGEMTEVASEMKKLAKQSWGSCYLTDRRGADPA"
BASE COUNT          696 a         1115 c          948 g          615 t
ORIGIN      
        1 gaaccgggct tcgagacgcc gggcacgata agacaggagg ccgagaaggc cctcgccgag
       61 ggcaagactt tctacaccca cagcctcggc atccccgagc tgaggcaagc agtctcccgc
      121 cactaccggg accgctacga cctcgacata tctcccgagc gcgtgatcat caccaacggc
      181 tcctcgggcg cattcatcct cctcttcgcg gcactcctcg agcggggccg gacccttgac
      241 atagccgacc ccgggtatcc ctgctaccgg aacatcgccc ttctcaccga ttgcgccgtg
      301 cgtgccctcc ctgtttcggc cgagaccaat tacgaggtcc ttcccgagca gcttggcggc
      361 tcgccggcac ccgatgtgct cgtcatcgcc aacccctcga acccgacggg aacgatctac
      421 cgcgccggcg ccctgaacgc cattcacaag aggctctctt cgcgcggggg cgtccttgtc
      481 gtcgacgaga tatactccgg cctcgtttat gatgcaccgt tctcctccag cgcggcaatc
      541 tccgaggacg tcatcgtcgt gaacggcttc tccaaggcct tcgccatgac tgggtggcgc
      601 cttggctgga tggtggtccc cccgggcctc gtccgcccca tccagaagct ggcgcagaac
      661 cttttcatcg cgccgccgtc catctcgcag tacgcggccc tcgcggcctt tgacgatgcc
      721 gacggtctcg cccgcatgtg cggtctgtac agggagagga gggacttcct cctgccgcgc
      781 cttcggggcc ttggattcga cattcccgtc acgcccgacg gggcctttta catttacgcc
      841 ggcatcgagc gcttcggcat ggacagcatg ctcttcgtgg aaagggcact tgccgaggca
      901 aaggtggcca tcaccccggg gtatgatttc ggccgcttcg gggccgcttc ccacgtccgt
      961 ttctcttacg cggacaggct cgagaacctc gtggaaggct gcgacaggct ggaaaggtgg
     1021 ctgtaaccgg gacgagcgcc ccgagaggtc gggcgaccgg gtattaggtt ttccggcttt
     1081 tggccgatag tattccaagg aggattgtgg tgccgaagat cgcccccggc agtctcgaac
     1141 cgggaatgaa gaccgcgaag cccgtcgtga acacaagcgg catggtcctt ctcggggaga
     1201 atacggagct caccccggag ctcatcgaca ggataaggaa catggaggtg gacggggtgt
     1261 ggatccaggg cctgtcgaag ccgtcggtcc cgatggagga ggtcgtcgcg gaacttgaca
     1321 ggcgcttccg cctcgtcggc ggccagcccc acatggatat cataaggaag gcggtccaaa
     1381 ggcacatcga gggcctctat gaatagcatg gacgtcaggg cggtcaggga aaagatcgaa
     1441 cacataaacg ctctccccac cattcccaag gtcctcaaca ggctcctcgc gctcatcgag
     1501 aacccccggg tctccctaaa cgagatctcg aacttcatct cctccgaccc cgccctcacc
     1561 acgaaggtgc tcaggatggt caactctccc gtatacggct tccccggaag gatctcttcc
     1621 gtgaaccagg ccgtcatact tctcggactc accgtcgtga aaggcctcct ccttggcgtt
     1681 tccgtcttcg agctcatgca gaagaccatg atcggcctct gggagcattc catagggacg
     1741 gccctgttct cacgcataat tgccgtgcgg gcgggccaca aggaacccga cgaggtctcc
     1801 gtcgacggcc ttctccacga tatcggcaag gtcttcctcg tgctccagtt ccccagcgag
     1861 taccagaggg ccctgacgca gacgaaagag gacggcatca tcatccacga ggccgaaaag
     1921 aaccatttca gcacgacaca cgccagcgtg ggcagctgga tggccaggaa atggcgcttc
     1981 ccggcaaagc tcatcgacgt catagagtac catcacaagc cacacctgac gaagagcacc
     2041 gccgtcgagt ccgccatcgt ccatattgcc gacatcctcg tgagggcccg gggtttcggc
     2101 tttcccggcg acgacctcat gacgcccgtc cagcccgagg catgggacct gctcaagctc
     2161 accgagaggg acatcctcga gatactcgag gaagccgagg actccctgca ggccacggaa
     2221 gacctgaccc tatgaacgag aagaagaccg tcgtcgtcat atcgcaggac gttgtcctct
     2281 cgggcataat agaccgcatc ctttcccggg actacggcgt cttcctcttc aggaacatcc
     2341 agtccgccat cgactacatc tacaattcgc cgccgaacct catcatcctc gacacctccg
     2401 aagaggacat ctactccatg gacgtcatca acaacctcaa gagcgacccc atcttctacc
     2461 agttgcccgt tctcgtcgtc atcgacgaca tcccgagcgt tcccgtctgg aaggaccttt
     2521 tcgtcgaaga ttacctgaag agggaggata tcgagcagga aggcgtcacc cgcgttgccc
     2581 tggcgatact gcgctcggag cgcatcgtgg aagtgaaccc tctcaccaag ctgccgggca
     2641 acatctccat aagcaggcag atacaggcaa ggatcgaggc gggcgatgtc ttcgccctcg
     2701 cctacgcgga cctggatcac ttcaagccct tcaatgatca ctacggcttt acaaggggcg
     2761 acgaggtgat acgcataacg ggccggctta tcctcaacat cgtcaagagc aaagagcccc
     2821 agaccgcctt cgtcgggcac ataggcggcg acgatttcgt tttcattaca tccgtcgacg
     2881 tcgtcgagga ggcctcggcc gacatcatcg acgccttcga caggatcacc cccagcctgt
     2941 acgacaggaa ggacagggac gcgggcttca tagagaccct cgaccgccag gggaacacca
     3001 gaaagttccc cgtaatcgcc ctttccatag gcatcgccac gaacaggacg cggtccttta
     3061 cccacttcgg ggagatgacc gaggtggctt cggagatgaa gaagctcgcc aagcagtcct
     3121 ggggaagctg ctacctgaca gaccgcaggg gagccgatcc ggcgtaggcc ggattgacgt
     3181 ggacgggcac acgggtctgc gcgctccgca cgcagaccct cattcccctt gcccaacccg
     3241 gcgatctggg gtaaaacctt cttttagatc gccagcaggg agagtatatg gtcggcatcg
     3301 tcaaagatcc tctctatctc gagcatgacc cgggcaacta ccacccggaa agtccggacc
     3361 gcctggggca catc
//
LOCUS       DAOPOA010000173         3367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_30632_length_3367_cov_4.071256, whole genome shotgun sequence.
ACCESSION   DAOPOA010000173 DAOPOA010000000
VERSION     DAOPOA010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30632_length_3367_cov_4.071256"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /gene="yedF"
                     /locus_tag="PKJ17_04685"
     CDS             <1..490
                     /gene="yedF"
                     /locus_tag="PKJ17_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOD75298.1"
                     /translation="VVVDNPTASENVRRFLGSRGYSTTVLQEGDVYRIEGCLQEGGTT
                     AQQAGAQKRVLVYVDTESMGRGDDGLGKILMRSFLATLKELEVRPWRVVFLNAGVRNV
                     TAGSEYLEILKELEGLGVEIISCGTCLDYFHLKDKVAVGRTSNMFEILTSFNEATNVI
                     RP"
     gene            520..1659
                     /locus_tag="PKJ17_04690"
     CDS             520..1659
                     /locus_tag="PKJ17_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOD75299.1"
                     /translation="MIYLDNAATSFPKPAETIRYLNDFVTHIGGNPGRSGHALSLEAA
                     RVIFEAREKLTAFIGMTDSERLIFTQNGTESLNMALLGLLGEGDHVVTTRMEHNSVMR
                     PLEFLSKERGVSYTAVPCSPEGFLDIGDIRAALKRNTAAVVINHGSNVTGTVQPLEGI
                     KDAVGKALLIVDACQTIGSLPIDMEVMKADILCFSGHKSLFSIQGVGALYVRSGIELT
                     PLKFGGTGSKSESIQHPTFLPDRYECGTPNTPGIASLLGGLTFIEQEGLGTIMDRKRA
                     LRRMLVEGLRAIRGVEVYGHKGPKDASFLATAAFNIKGLLPSDVGYELNRRSMYVRIG
                     LHCAPMAHQTVGTFPNGSLRASPGYFTQEDDIRAFIEAVSEIAEG"
     gene            2116..2562
                     /locus_tag="PKJ17_04695"
     CDS             2116..2562
                     /locus_tag="PKJ17_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD75300.1"
                     /translation="MLWTDGFGMFGRGLDPWSEFQRIENEMNRVLSRFVSPSTGDFPA
                     VNIWSDGEETLVTTEIPGIESGAIDISVAGKTLILKGAREPDQVEEGSSYHRRERWYG
                     QFSKAIELPFTIDADRVAADFSNGILTIRLPRSEAEKPRKITIKSA"
     gene            2574..2978
                     /locus_tag="PKJ17_04700"
     CDS             2574..2978
                     /locus_tag="PKJ17_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD75301.1"
                     /translation="MADKTKDIPKKEAAETDVVERTRAGKIYNPDVDIMEGKDKIIVI
                     ADMPGVNENSVEVTLDDNVLTIYGRVDWKAPEKMKLIHAEYGVGDFQRIFTISGEINR
                     EKIQARVKNGVLRLVMPKNDAPKMRKIAVKAG"
     gene            3011..>3367
                     /locus_tag="PKJ17_04705"
     CDS             3011..>3367
                     /locus_tag="PKJ17_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD75302.1"
                     /translation="MTTRRVSSSVKRARGAARLSEENPLLSLRSNIDRLFDNFLSGLN
                     ISPFTATASMFNPSIDIADSGKELRVTVELPGMDENDIDVSVTQSSITIRGEKRDETE
                     EKGNSYHRMERLYGSFT"
BASE COUNT          823 a          901 c          993 g          650 t
ORIGIN      
        1 ggtcgtcgtg gacaatccca ctgccagcga gaatgtgcga cgtttcctgg gatcacgggg
       61 ttattcgacg accgtcttac aggaaggcga cgtttacagg atcgagggct gtctgcagga
      121 aggtggcacg acggcacagc aagccggggc gcagaagaga gtcctggtct acgtcgacac
      181 ggagagcatg ggaagagggg atgacgggct cggcaagatc ctgatgcggt cctttttggc
      241 cacgctcaag gagttggagg tccgcccctg gagggttgtg ttcctgaacg cgggcgtcag
      301 gaacgttacg gccggctccg aatacctgga gatcctgaag gagttagagg gactcggcgt
      361 ggagatcatc tcatgcggga cctgcctcga ctattttcat ctcaaggata aggttgcggt
      421 ggggaggaca agcaacatgt tcgagatcct gacctcattc aacgaggcca ccaacgtgat
      481 caggccctag ccggcacgtc accagggcag gggtcatcca tgatctatct ggacaacgcg
      541 gcgacatcgt tccccaaacc agcggaaacc atccggtatc tcaatgattt cgtaacacat
      601 atcggcggca acccgggcag gagcggccat gcactctctc ttgaggcggc gagggtgatc
      661 ttcgaggcac gggagaagct caccgccttc atcgggatga ccgactcgga gcgcctcatt
      721 ttcacccaga acggaacgga atcgctgaat atggcccttc tcggcctttt gggggagggt
      781 gaccatgtcg tgaccaccag gatggagcat aactctgtca tgaggcccct ggagttcttg
      841 agtaaagaaa gaggagtatc gtacaccgcc gttccctgtt cgcccgaggg attcctggac
      901 atcggcgaca tccgcgccgc ccttaaaagg aatactgcag ccgttgtgat caatcacgga
      961 tccaatgtca ccggtaccgt tcagcccctc gagggaatca aggatgccgt cggtaaggcc
     1021 ttgctcatcg tggatgcctg ccagaccatc ggctccctgc ccatcgacat ggaagtgatg
     1081 aaggcggata tcctctgttt ttccggtcac aagtcgctct tctccatcca gggagtggga
     1141 gctctctacg tgcggtccgg catcgaactc acacctctga aattcggggg aacgggcagc
     1201 aagtcggaat cgatccagca tcccacgttc ctgcccgaca gatacgagtg cgggacaccg
     1261 aacacaccgg gcatcgcgtc gctcctcggc gggctgacat tcatcgaaca ggaggggctc
     1321 gggacgatca tggataggaa gcgcgcactt cggcgcatgc tcgttgaggg cttgagggct
     1381 atccgaggtg tcgaggtgta cggtcacaag ggtccgaaag acgcatcgtt cctggcaacc
     1441 gcggcattca acatcaaagg tctgctccct tccgacgttg ggtacgaact caacaggagg
     1501 tcgatgtacg ttcgcatcgg ccttcactgc gcgccgatgg cgcaccagac ggtcggcacc
     1561 ttcccgaacg ggtccctccg ggcatcaccg gggtatttca cccaggagga cgacataagg
     1621 gcatttatcg aggcggtcag cgaaattgcc gaaggctaag ggcttcattc tcttatttcg
     1681 ttcgatccac gatgtcctgc gagcggaaaa aatgctcaaa aagcaggacg tgatacacga
     1741 actcgtaccg gtaccaagaa atttgagttc cgactgcggc atgtgtatca ggcttgacag
     1801 cgatgcggca gatatacaac ggtgcctggg tgatattgag atcgacagga gctttgtctt
     1861 cgacggcgag agctaccaac aggtcgaact atgatagccg cggtcgtcga cgcctgcccc
     1921 gtggtccctg tatccctgcc gcttgccctc caggcgggaa acgggcagca ccgaacgtgg
     1981 ccacctcctt tccagctgta ccaataacct attagattca tccacttaga acccatccac
     2041 gccacttgac aaaagatatc cgagtgaaca gattaatttc acaaagagga aatatgcaac
     2101 aaggaggctg ataccatgtt gtggacagac ggttttggca tgtttggcag agggcttgac
     2161 ccgtggtccg agtttcaacg gatcgagaac gagatgaacc gcgtcttatc caggtttgtc
     2221 tcgccctcga caggtgactt tcccgccgtc aacatatgga gcgatggcga ggagaccttg
     2281 gtgacaacgg agatcccggg tatcgaatca ggcgcgatag acatctcggt cgcaggcaag
     2341 acccttatac tcaagggtgc cagggagcct gaccaggtgg aggagggtag ttcctatcat
     2401 cgccgcgagc gatggtacgg ccagttcagc aaggccatcg aacttccctt cacgatcgac
     2461 gccgaccggg tcgcggccga cttctccaat ggcatcctca cgatcagatt gccgcgatcg
     2521 gaggcggaga aaccgagaaa gatcactatc aaatccgcgt aaggaggcgc acgatggctg
     2581 acaaaaccaa agacatacca aagaaagagg ccgccgaaac ggatgtggtg gagaggaccc
     2641 gagcgggcaa gatctacaac cccgatgtcg acatcatgga aggtaaggac aagatcatcg
     2701 tgatcgccga catgcccgga gtcaacgaga actccgtgga ggttacactg gacgacaatg
     2761 tcctgaccat ctacggaagg gttgattgga aggcgcctga aaagatgaaa cttatccacg
     2821 cggaatacgg agtgggcgac ttccagagga tcttcaccat atcgggcgag atcaacaggg
     2881 aaaagatcca ggcaagagtg aagaacggtg ttctcaggct ggtcatgccc aagaatgacg
     2941 ctccgaagat gaggaagatc gctgtcaagg ctggttgaac aatgacgtga gacacacagg
     3001 aggtacgatc atgacgacaa gaagagtgtc atcgtcggtg aagagagcac ggggcgccgc
     3061 ccggctgtcg gaagagaatc cccttctgtc gttgaggagc aacatcgaca ggctgttcga
     3121 taatttcctc tcagggttga atatcagtcc tttcaccgca acagcatcga tgttcaaccc
     3181 gagcatcgat atcgcggaca gcggcaagga actcagggtg accgtcgaac tgcccggcat
     3241 ggacgaaaat gacatcgatg tatctgtcac ccagtcctct atcaccattc ggggagagaa
     3301 aagggacgag acagaggaga aaggcaatag ctaccatcgt atggaacggc tctacggctc
     3361 ttttacg
//
LOCUS       DAOPOA010000174         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_30702_length_3362_cov_3.911702, whole genome shotgun sequence.
ACCESSION   DAOPOA010000174 DAOPOA010000000
VERSION     DAOPOA010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30702_length_3362_cov_3.911702"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="PKJ17_04710"
     CDS             complement(<1..1413)
                     /locus_tag="PKJ17_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD75303.1"
                     /translation="MDRTGKVLVIGGGIAGMEASLNLVEAGFKVYLADRKPNIGGNMA
                     QLDKIFPTNDCSMCVMAPKLVEVGKNPNIELVMNSEVFRLEGGPGHFRVTLRTRPRRV
                     DPGRCTSCASCAAACPLEVGNVYNEEMSLRSAAFINFPQAIPSTYMIDRQLSPCVYTC
                     PINLNARDYIGLIAEGRFLEALDLIREKLPFPGIIGRICAHPCESACLRGKEVDQPLA
                     ICALKRFVADYEVGRREAPLPVTQTEKEQKVAIIGAGPAGFACAVELRKAGFCVTVFD
                     ANDKPGGMLYAGVPAYRLPRDVLAREFCIVERMGAKLTMNTRIGRDIPLEEVLSSHDA
                     TFIASGAHGRRKLGLENEDARGVTSALEFLKDVNTGKEASPGRRVFVVGGGNVAMDTA
                     ITARRLGAAEVHIVALEKWDDMPAHRWEIDQAIEEDIVFHNAWGPLRITAEEGRLTGL
                     ELARCVRVFNEEGLFDPLYDT"
     gene            complement(1455..1838)
                     /locus_tag="PKJ17_04715"
     CDS             complement(1455..1838)
                     /locus_tag="PKJ17_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOD75304.1"
                     /translation="MSRSGNDHGPDAGRIGTLEYVRRHDPDGAEKLQRLLERKDVLTA
                     GNSYGERMTERQFSLAFDPLLTRAVERSQILESLGTGPASISDLARTLGMTARTVFDH
                     LKELSRRDLVEISGYTDRCALYRRK"
     gene            complement(1835..2707)
                     /locus_tag="PKJ17_04720"
     CDS             complement(1835..2707)
                     /locus_tag="PKJ17_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOD75305.1"
                     /translation="MITLSYFPGCSLTSSSSFYDRSLRRVLAFYEVGLRELEDWSCCG
                     AAAAPAMRQELSDVLNARNIAIAEKSGLHLLAPCSACYARTRASALKIAGDRSTRDMV
                     NEALSPMSVKGSLEVRNIIETFLQYVGVEKIAASVAYGIGSMRVVPYYGCLLTRIMGA
                     PSSDDVEDPSGMDVLLDALGAVVVPWQFKTECCGAGSTATDRDRTTRLSGRIMAMAKK
                     AGANAVVTTCPLCQLNLDLTTHARGDLAGIPVLFLTEVFELALFGSTPDSKHHLISMA
                     DIEGSVEILGGDSA"
     gene            complement(2704..3240)
                     /locus_tag="PKJ17_04725"
     CDS             complement(2704..3240)
                     /locus_tag="PKJ17_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD75306.1"
                     /translation="MPLPSFLDEIETRSGVTVSVCYQCCRCTNGCPVAGEMEIVPHRV
                     IGYILSGEREKVLASTSPWKCLQCATCSLRCPNGIDVARVFETLRQISLASGFAAKND
                     VAHFDDLIIDSVQRHGRFAELETIMRYRLSKGQFMKDAAMGLDMLRKGRIGLSSHNIR
                     GRKELGAIIRRMRGHRDR"
BASE COUNT          602 a         1024 c         1050 g          686 t
ORIGIN      
        1 cgtgtcgtaa agaggatcga aaagaccctc ctcgttgaag acgcggacgc agcgggcgag
       61 ttccaaccct gtgagcctgc cctcctcggc ggtgatcctc aaagggcccc aggcgttgtg
      121 aaagacgatg tcctcctcga tcgcctggtc gatctcccat cggtgggcgg gcatatcgtc
      181 ccatttttcg agggccacga tatggacctc ggcggccccc agcctgcgcg ccgtgatggc
      241 cgtgtccatg gcgacgttcc cgcctccgac gacgaagacc ctcctgcccg gcgaggcctc
      301 tttccccgtg tttacgtctt taaggaactc gagagcgctc gtgacccccc tcgcgtcctc
      361 gttttcgagc cccagcttcc gcctgccgtg ggcaccggag gcgatgaagg tcgcgtcgtg
      421 ggacgaaagg acctcctcga gggggatatc cctgcctatc cgtgtgttca tggtgagctt
      481 tgcgcccatc cgctccacaa tgcaaaactc ccgggcaagg acgtccctcg gaagcctgta
      541 ggcgggcacg ccggcataga gcatcccgcc cggcttgtcg ttggcgtcga agaccgtcac
      601 gcagaagccg gccttcctca gctctaccgc gcaggcgaag ccggcgggtc cggcgcctat
      661 gatcgcgacc ttctgctcct tctcggtctg ggtcacggga agaggcgcct cgcgcctgcc
      721 cacctcgtag tcggcgacga agcgcttgag ggcgcagatg gcaagaggct ggtccacctc
      781 tttgcccctt aagcacgcgc tctcgcaggg atgcgcgcag atcctcccga tgatgccggg
      841 aaagggcagc ttttcccgga tgaggtcaag ggcctcgagg aacctgcctt ccgcgatgag
      901 cccgatgtag tcgcgggcgt tgagattgat gggacaggtg tagacgcagg gggagagctg
      961 tctgtcgatc atgtatgtcg aaggtatggc ctgcgggaaa ttgatgaatg ctgcgctgcg
     1021 aagggacatc tcttcgttgt acacgttgcc gacctccagc gggcacgccg ccgcgcagga
     1081 agcgcaggac gtgcagcgcc ccgggtcgac gcgtctcggc cgcgtgcgga gggtgacccg
     1141 gaagtgcccc gggccccctt ccaggcggaa gacctcggag ttcatgacga gttcgatgtt
     1201 ggggttcttg cccacttcga cgagcttcgg cgccatgacg cacatggaac agtcattggt
     1261 gggaaatatc ttgtcgagct gggccatgtt cccgccgatg ttcggcttgc ggtcagcgag
     1321 atagaccttg aagccggcct cgacgaggtt aagggacgcc tccatgccgg caatgcctcc
     1381 ccctatgacg aggacctttc ccgtcctatc catgtggccc ctcgcccggt tgcgtgaacg
     1441 tccgtatgcc gcggtcattt tctcctgtac aacgcgcatc ggtctgtgta tcccgagatc
     1501 tcgacgagat ccctgcgcga gagctccttg aggtgatcga acacggtccg cgccgtcatc
     1561 cccagtgttc gtgccaggtc cgatatggat gccgggcccg tcccgaggct ttccagtatc
     1621 tgcgaacgct ccacggcgcg ggtgaggagg ggatcgaagg cgagggagaa ttgacgctcc
     1681 gtcatgcgtt cgccgtagga gtttcccgcc gtgagaacgt ccttcctctc aaggagcctt
     1741 tggagtttct cggccccatc ggggtcatga cgcctcacgt attccagtgt cccgatacgc
     1801 cccgcgtcgg gtccatggtc attaccggat ctgctcatgc gctgtcgcct ccgagtattt
     1861 ccaccgatcc ttctatgtcg gccatggata tcagatggtg tttgctgtcc ggggttgaac
     1921 cgaagagagc gagttcgaag acctccgtca ggaagaggac gggaatgccg gcgaggtcgc
     1981 cccttgcgtg cgtggtcagg tcgagattga gctggcacag cggacaggtg gtgacaactg
     2041 catttgcccc ggcttttttc gccattgcca tgatgcgccc ggaaagccgc gtcgtccggt
     2101 ccctgtccgt cgccgttgac cccgctccgc agcactccgt tttgaattgc cagggaacga
     2161 cgacggcccc gagggcatcg agaaggacat ccatgccgga agggtcttcg acgtcgtcgg
     2221 aggagggcgc gcccatgatg cgggtgagaa gacacccgta gtaaggcacg acgcgcatgg
     2281 agccgatgcc gtacgcgacg gaggcggcga tcttctcgac cccgacgtac tggagaaatg
     2341 tctcgatgat gttcctgacc tccagggaac ccttcacgga cattggggag agggcctcat
     2401 tgaccatgtc ccgcgtcgac ctgtcgccgg ctatcttgag cgcgctcgcc cgcgtgcggg
     2461 cgtagcaggc ggagcagggg gcaaggaggt gaaggccgga tttctcagct atggcgatgt
     2521 tccgggcatt caacacgtcg gacagttcct ggcgcatggc gggcgccgcg gcggcgccgc
     2581 agcacgacca atcctcaagc tccctgagtc ccacctcgta aaaggccagg accctcctga
     2641 gggaacggtc gtagaaagag ctggacgatg tcagggaaca gcccggaaag taggataggg
     2701 tgatcatctg tcccgatgtc cccgcatgcg tcgaattatt gcgcccagtt ctttcctgcc
     2761 gcggatgttg tgggaagaca ggccgatccg tcccttcctc agcatgtcga gccccatggc
     2821 ggcatctttc atgaactgtc ctttcgagag gcggtagcgc atgatcgttt ccaactcggc
     2881 gaagcgcccg tgcctttgta cactgtcgat gataaggtcg tcgaaatgcg cgacgtcgtt
     2941 ctttgcggca aagccggagg cgagggagat ctgccggagg gtttcgaaga cgcgggcgac
     3001 gtcgatgcca ttggggcatc gaaggctgca ggtggcgcac tgaagacact tccatgggga
     3061 tgtcgacgca aggacctttt cgcgctcgcc gctcaggata tacccgataa ccctgtgggg
     3121 gacgatctcc atctccccgg caacggggca tccgttggtg cagcggcaac actggtaaca
     3181 caccgagact gtcacccctg agcgtgtttc tatctcgtcg aggaaagaag ggagcggcat
     3241 atcagacttt tattcttaaa ttttatctcc atgatgtcaa atggattttc cttcccgccg
     3301 ccgccccctt gtcttccctc ttcattcccg ttgtggtata tctaagagtc atggaaagac
     3361 cg
//
LOCUS       DAOPOA010000175         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_30778_length_3355_cov_3.610606, whole genome shotgun sequence.
ACCESSION   DAOPOA010000175 DAOPOA010000000
VERSION     DAOPOA010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30778_length_3355_cov_3.610606"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1359
                     /locus_tag="PKJ17_04730"
     CDS             115..1359
                     /locus_tag="PKJ17_04730"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOD75307.1"
                     /translation="MRPEKLAQKAKGASDTLAHASTDEKNRVLERLAEALGKNQDYLY
                     EENMKDVRAAEKEGMAKSLVDRLRIDDKLIREMQGSLRDVAALPDPVGEIVKVWKRPN
                     NLLVGRMRIPIGVILIIYESRPNVTIEAFSLCLKSGNCVILKGGSEAYHSNRALYRLI
                     VETLKGSKISPDAVQFVDTADRDYIYTLLEREEEIDLVIPRGGEALIRNIVERSRIPV
                     LKHYKGVCHIYVDDEADLTMAVDVAVNAKVQKPATCNALETLLVHEKVAKSFLPKVHR
                     ELSRQGVTMKGCPKTVAILKDIGRATKEDWPMEYLDLTLAVTVVADMDEAVDHIRKYG
                     SSHTEAIVTANYERAWKFLREVNSSLVLVNASTRLNDGFQLGLGAEMGISTTRLHAFG
                     PMGLEELTVTKFIAFGDGQLRT"
     gene            1370..>1631
                     /locus_tag="PKJ17_04735"
     CDS             1370..>1631
                     /locus_tag="PKJ17_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOD75308.1"
                     /translation="MATGVFGGTFNPVHVGHLRVAEEIREDFHLDSVYFIPSHVPPHK
                     EAAGGLPGERLRMLKAAVRGNASFKVSDMEIRRGGVSYNIDTL"
     assembly_gap    1632..1641
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1642..2013
                     /locus_tag="PKJ17_04740"
     CDS             <1642..2013
                     /locus_tag="PKJ17_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75309.1"
                     /translation="RFDDIHFIIGVDAFRQIDTWHHYEELFYHTGFIVMTRPASLPAD
                     IPEMVPEAVRVKLTALGDGVYRHESGKKIYLRAVTQMDISSTKVRDLLRGERSIRYLV
                     PPGVEKIIIERGLYRSSDETR"
     gene            2015..2374
                     /gene="rsfS"
                     /locus_tag="PKJ17_04745"
     CDS             2015..2374
                     /gene="rsfS"
                     /locus_tag="PKJ17_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOD75310.1"
                     /translation="MVEACGRLADDKKALDVVVMDLTGITDIADHFVIAGGTSERHVR
                     TIADGIREGMKQEGARPLAVEGYDEGRWVILDYQSVVVHIFLEQLRELYDLESLWIEA
                     KRHRIKNENKNTEVGNE"
     gene            2367..2720
                     /locus_tag="PKJ17_04750"
     CDS             2367..2720
                     /locus_tag="PKJ17_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOD75311.1"
                     /translation="MNKEKVEFYRKTLLKMRENILNKAKKLKEDSYNLGTDGIQDMAD
                     AASNTYNADILMSISDNDLTILKDIDNSLDKLSKGSYGICEECEEKISEKRLEANPVA
                     RYCITCKRQMEEKGL"
     gene            2724..>3355
                     /locus_tag="PKJ17_04755"
     CDS             2724..>3355
                     /locus_tag="PKJ17_04755"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD75312.1"
                     /translation="MRYKIFFFVLALFLVFYFYIAHLNPENVKFYYGGARPIELSLAE
                     FIIASFCIGVIISIIISFFYDVKTTITSWMARRSERKSEEYLGLLEKARHYDLKGDRE
                     KAIEHLDRLSRRNPGREETYIALADIYVSMEDYEKARETLDLAETTLGKTENVLFKKV
                     KVNMVSREYTSNEGILKEILRLNESSLKAARLLRDLYIWKKDWDGAYDVEV"
BASE COUNT          855 a          896 c          991 g          603 t
ORIGIN      
        1 ctactcctcc tccgagatgg acaggataaa aggcctgaaa agtcttgata tagagaagga
       61 gcttggatat aaatacacgg aagaaatggt ccacagggac aacatggtgg tgatatgaga
      121 ccggaaaagc ttgcccagaa ggcgaaaggc gcatcagata ccctggcgca cgcatccacg
      181 gacgagaaga acagggtgct cgaacgcctt gccgaggcgc tcgggaagaa ccaggattac
      241 ctgtacgaag agaacatgaa ggacgtgcgc gccgccgaga aggaaggcat ggcaaagagt
      301 ctcgtcgacc gcctccgcat cgatgacaaa ctcatcaggg agatgcaggg gagcttgaga
      361 gatgtggcgg ccctcccgga ccccgtcggg gagatcgtca aggtctggaa aaggcccaac
      421 aatcttctcg tgggcaggat gaggatcccc atcggcgtca tcctcatcat ctacgagtcc
      481 cgtcccaacg tgaccataga agccttttcg ctgtgtctca agagcggcaa ctgcgtcatc
      541 ctgaagggag ggtcggaggc gtaccattcc aacagggccc tctaccggct catcgtggag
      601 actctgaagg ggtcgaagat atcccccgat gccgtccagt tcgtggacac cgcggaccgc
      661 gactacatct acacccttct cgaaagggag gaggaaatag acctcgtcat acccaggggg
      721 ggcgaggccc ttatccgcaa catagtggaa cgttcccgca tacccgttct caaacattac
      781 aagggggtat gccacatata cgtcgacgac gaggccgatc tcacgatggc cgtcgacgtc
      841 gccgtcaacg ccaaggtcca gaagccggcc acatgcaacg ccctcgagac ccttctcgtt
      901 cacgagaagg tcgcgaagtc cttcctcccg aaggtgcaca gggagctctc ccggcagggc
      961 gtcacgatga agggttgccc gaagacggtg gccatcctaa aggatatcgg cagggccacg
     1021 aaggaagact ggcccatgga gtacctcgac ctcacgctgg ccgtcacggt ggtcgcggac
     1081 atggacgagg ccgtcgacca tatcaggaag tacgggtcga gccataccga ggccatcgta
     1141 acagcgaact acgaaagggc ctggaagttc ctccgcgagg tcaactcctc gctggtgctc
     1201 gtcaacgcct cgacacggct caacgacggt ttccagctcg gcctcggcgc ggagatgggc
     1261 atcagcacaa cgaggctcca tgccttcggg cccatgggct tagaggagct cacagtgacg
     1321 aaattcatcg ccttcggtga tgggcaattg cggacgtaag gacggtacaa tggcaactgg
     1381 tgtctttggc ggaaccttca acccggtgca cgtggggcac ttgcgcgtcg ccgaggagat
     1441 cagggaggat ttccacctgg acagcgtcta tttcatccca tcccatgttc ctccccacaa
     1501 ggaagccgcg ggcgggctcc ccggcgagcg cctgaggatg ctgaaggccg ccgtcagggg
     1561 caacgcctcc tttaaggtct ccgacatgga gatacggcgg ggaggggtat catacaacat
     1621 cgacacgctg annnnnnnnn ncgtttcgat gacatccatt tcatcatcgg cgtcgacgcc
     1681 ttccggcaga tagacacgtg gcaccattac gaagagctct tctatcatac cggtttcatc
     1741 gtgatgacga ggcccgcctc gctaccggcc gatataccgg aaatggtgcc cgaggcggtg
     1801 cgcgtgaaac tgaccgccct cggagatggt gtttaccgcc acgaatcggg caagaagata
     1861 taccttcgag cggtgacaca gatggatatc tcctcgacga aggttaggga tctcctcagg
     1921 ggcgaacggt ccatcaggta tctcgtgcca cccggcgtgg agaagataat aattgaaagg
     1981 gggttgtaca ggtcctcaga tgaaacccgg tgatatggta gaagcgtgcg gaaggctggc
     2041 agacgacaag aaggcccttg acgttgtcgt tatggacctg acaggtatca cggacatcgc
     2101 tgatcacttc gttatagccg gggggaccag cgaacgacat gtgaggacca tcgcggacgg
     2161 gatacgggaa ggcatgaaac aggagggagc gaggccgctc gccgtcgaag ggtacgacga
     2221 aggccgctgg gtcatcctcg attatcagag cgtcgtcgtc cacatctttc tcgaacagct
     2281 cagggagctc tacgatctgg aaagcctgtg gatcgaagcg aaaaggcata ggatcaagaa
     2341 cgaaaataag aacacagagg tggggaatga ataaggaaaa agttgaattc taccgcaaga
     2401 cacttctcaa gatgcgcgag aacatactca acaaggcgaa gaagctgaaa gaggattcat
     2461 ataacctcgg gacggacggg atccaggaca tggccgacgc ggcaagcaac acctataacg
     2521 cggacatcct gatgagcata agcgacaacg atctcaccat actcaaggac atcgacaact
     2581 ccctcgacaa actgagcaag ggctcctacg gcatatgcga ggaatgcgag gagaagatca
     2641 gcgagaaaag acttgaagca aatcctgtgg cacggtattg tataacctgt aagcgacaaa
     2701 tggaggaaaa gggtttatag cggatgcggt acaagatatt ctttttcgtt ctggcgcttt
     2761 ttcttgtctt ctatttctac atcgcgcatc tgaaccccga gaatgtgaag ttctattacg
     2821 gaggggcacg gccgatagag ctctcgctgg ccgagttcat catagcctcc ttctgtatcg
     2881 gcgtcatcat atcgatcatc atcagttttt tctatgatgt gaagaccacg ataacatcgt
     2941 ggatggccag gcgctccgag cggaagagcg aggagtacct ggggctcctg gaaaaggccc
     3001 ggcactacga cctcaagggc gaccgggaaa aggccatcga acatctcgac aggctctcgc
     3061 gccgcaaccc gggccgcgag gaaacataca tcgcccttgc cgatatctac gtttccatgg
     3121 aagattacga gaaggcgagg gagaccctcg atctcgcgga gacgaccctg gggaagacgg
     3181 agaacgtcct tttcaagaag gtcaaggtga acatggtctc cagggaatac accagcaacg
     3241 agggcatact gaaggagatc ctgcggctga acgaatcgag cctcaaggca gcgcggctgc
     3301 ttcgggacct ctacatatgg aagaaggact gggacggcgc ctatgacgtg gaagt
//
LOCUS       DAOPOA010000176         3326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_31166_length_3326_cov_3.986243, whole genome shotgun sequence.
ACCESSION   DAOPOA010000176 DAOPOA010000000
VERSION     DAOPOA010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31166_length_3326_cov_3.986243"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKJ17_04760"
     CDS             <1..473
                     /locus_tag="PKJ17_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD75313.1"
                     /translation="DLIFLPDYYNKVALISRQIREKGIRSALLGGDGWDSPDLLKIGG
                     NAIMGGYFVNHYSPDRKDAVADAFIKKYKARHGIIPDAFAALAYDAAGILLKALDAAK
                     KPVSEEILKALAATKNYKGVTGTISFDAKGDAIKSAVILKVDKTGFTYVTTVNP"
     gene            546..1448
                     /locus_tag="PKJ17_04765"
     CDS             546..1448
                     /locus_tag="PKJ17_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD75314.1"
                     /translation="MEHASYIISQVINGLQLGAVYALIALGYTMVYGILRLINFAHGD
                     IFMLGAFFAYFLISRFGTPIYLVFFATMAMCALAGYFMEKIAYKPLRNAPRISLLITA
                     VGVSLFLEYFCSLKAIFTPDYIGFPRPFEVSAWNFSLGGIDFSFTNVQVLIFAITFFT
                     LALLYLLVYRTKQGTAMRAVSHDQETAALMGINIDHTVSLTFMVGAALAGVGGILYGI
                     AYPQINVFMGIMPGIKSFIAAVLGGIGIIHGAVVGGLIVGVSEVIVSAFLSSTYRDGV
                     IFVILLIVLLVKPSGLFGKRIDKV"
     gene            1445..2389
                     /locus_tag="PKJ17_04770"
     CDS             1445..2389
                     /locus_tag="PKJ17_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD75315.1"
                     /translation="MKFFLESRRLILLAAGIYIVLKLLFSFGIVSEYIQQITIYVFIV
                     VLAATGLNVIYGYTGQFSLGHAAFYGIGAYTSAYLVKTLGLDSVFALPATLLAGGIMA
                     GLVACVIGIPILRLRDDFLGIATLGFGILVRVFFDNSDRISETLGGSRGFTGIAKLTN
                     LEITFVVTVFLLIVTRNLISSRYGLFWRCIKDDETASASVGIDTTRMKLMAFSYGCFL
                     AGVAGALYAHLYTFLHPSNFDFLRSIDFLIIVILGGMGNIAGTIWAGVLWVGFIEGLR
                     IVLPSNVLDLRWVFIPLLLIMLMMWRPYGLMSKKKGNG"
     gene            2419..3189
                     /locus_tag="PKJ17_04775"
     CDS             2419..3189
                     /locus_tag="PKJ17_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD75316.1"
                     /translation="MTAMLEVERLEKSFGGVRAVDGFSFHVDRGETLGIIGPNGAGKT
                     TVFNLITRFYTPDGGRVRFMGKLITHLPPYRLARLGIARTFQNLRLFKDLTVLENITS
                     AHLTGHSYSPFAGIFRTRRHLRIEREARENAMMLLNFLGLGEKEQAPAGSLPYGEQRR
                     LELARALAIRPKLLLIDEPGAGMNPREIQDLVITLREVKERFDLTIVMIEHQMGLVMN
                     ISDRILVMDFGEKIMEGTPSEVRKDRRVIEAYLGEEPV"
BASE COUNT          649 a         1051 c          921 g          705 t
ORIGIN      
        1 cggacctcat cttcctgccc gactattata acaaggtcgc cctcatctcc aggcagatac
       61 gtgagaaagg cataagatcg gcccttctcg gcggtgacgg ctgggattcg cccgacctcc
      121 tcaagatagg gggcaacgcc atcatgggtg gatatttcgt gaaccactat tcacccgacc
      181 gcaaggacgc cgtcgccgac gccttcatca agaaatacaa ggcccgccac ggcatcatcc
      241 ccgacgcctt cgcggccctg gcttacgacg cggcagggat cctcctgaag gccctcgacg
      301 cggccaaaaa accggtctcc gaggagatcc tcaaggccct cgcggcaacg aagaactaca
      361 agggcgtcac gggcaccatc agcttcgacg ccaagggcga cgccatcaag tcggcggtca
      421 tcctgaaggt ggacaagacc ggcttcacct acgtgacgac ggtgaatcca taaggacgag
      481 gggcgccccg ggccccccgg gcccttttcg tcccctctcc ggatcttagg acacagtatc
      541 gcacaatgga gcatgcgagc tacataatct cccaggtcat caacggcctc cagctgggcg
      601 ccgtgtacgc cctcatcgcc ctcggctaca ccatggtcta cgggatcctg cgtctcataa
      661 acttcgcgca cggggacatc ttcatgctcg gcgccttctt cgcctatttc ctcatctccc
      721 gtttcggcac gcccatctac ctcgtcttct tcgcgacgat ggccatgtgc gccctggccg
      781 ggtacttcat ggaaaagata gcctacaaac cccttcggaa cgcgccgagg atatcccttc
      841 tgatcaccgc cgtcggcgtg tcgctcttcc ttgaatactt ctgcagcctg aaggccatct
      901 tcacaccgga ctacatcggc tttccccgcc ccttcgaggt gtcggcctgg aacttttcgc
      961 tcggcgggat cgatttctcc ttcacaaacg tgcaggtgct catcttcgcc atcacgttct
     1021 tcactctcgc gctcctgtac cttctcgttt acagaacaaa acagggcacg gccatgcgcg
     1081 ccgtgtccca tgaccaggag acggcggcgc tcatggggat caacatcgac cacaccgttt
     1141 ccctcacttt catggtggga gcggccctcg ccggcgttgg gggcattctt tacggaatag
     1201 cctatcccca gatcaatgtc ttcatgggga tcatgccggg gataaagtcc ttcatcgcag
     1261 ccgtgcttgg gggcatcggc atcatccacg gggccgtcgt cggaggcctg atcgtcggtg
     1321 tgtccgaggt catcgtgtcg gccttcctct cctcgacgta tcgggacggg gtcatcttcg
     1381 tgatccttct catcgtcctt ctcgtcaagc cgagcggcct cttcggcaaa aggatcgaca
     1441 aggtatgaag ttcttcctgg agtccaggag actgatcctt cttgccgcgg gcatctatat
     1501 cgtccttaag ctcctcttca gcttcggcat cgtctcagag tacatccagc agataaccat
     1561 ctatgtcttc atcgtggtgc tcgcggcgac gggcctcaac gtcatctacg gctataccgg
     1621 tcagttctcc ctgggccacg cggcgttcta cgggatcggg gcgtacacct cggcctatct
     1681 cgtcaagacc ctggggctcg acagcgtctt cgccctcccc gccacactcc tcgccggggg
     1741 catcatggcc gggctcgtcg cctgcgtcat cgggataccg atcctgaggc tccgggacga
     1801 cttcctcggg atcgccaccc tgggtttcgg tatactggtg cgggtcttct tcgacaattc
     1861 cgacaggatc tcggagacac tggggggttc acggggcttc acgggcatcg caaaactcac
     1921 caacctggag atcaccttcg tcgtcaccgt cttccttctc atcgtcacga ggaacctcat
     1981 ctcttcgcgg tacgggctct tctggcggtg cataaaggat gacgagacgg cgagcgcctc
     2041 ggtcggcatc gacaccacgc gcatgaagct catggcgttc tcttacggct gctttctcgc
     2101 cggcgtcgcc ggggccctct atgcccatct ctacaccttt ctccacccct ccaatttcga
     2161 tttcctcaga tccatcgatt tcctgatcat cgtcatcctc gggggcatgg ggaacatagc
     2221 gggcacgatc tgggcgggcg tcctctgggt gggtttcata gaaggcctga ggatagtcct
     2281 accgtcgaac gtgctcgatc tcaggtgggt cttcattcct cttcttctca tcatgctgat
     2341 gatgtggagg ccttacgggc taatgagcaa aaagaagggt aatggttgat gggtcacggg
     2401 tcatggggag aagacgggat gacggcaatg cttgaggtgg aaagattgga gaagagcttc
     2461 ggcggggtcc gggctgtgga cgggttctcc tttcatgtcg acagagggga aactctgggt
     2521 atcatcggtc ccaacggggc gggaaagacg acggtcttca acctcatcac gcggttctat
     2581 acacccgatg gcggccgcgt acgcttcatg ggaaaactca tcacacacct tcccccctac
     2641 agactggcac gcctggggat cgcccggaca tttcagaatc tcaggctttt caaggacctc
     2701 accgtcctcg agaacatcac atccgcccac ctgaccgggc acagctacag tcccttcgcc
     2761 ggcattttca ggacccgaag gcacctccgt atcgaaagag aggcacggga gaatgcgatg
     2821 atgcttctta atttcctcgg cctcggggaa aaggagcagg cccccgccgg cagcctgcca
     2881 tacggcgagc agcgcaggct ggaactggcc agggcgctgg ccatacggcc gaaactgctc
     2941 cttatagacg aacccggcgc gggtatgaac ccccgcgaga tacaggacct cgtcatcacc
     3001 ttgcgggagg tgaaggagag gttcgatctc accatcgtca tgatcgagca ccagatgggt
     3061 ctcgtcatga acatatccga caggatcctc gtgatggact ttggagagaa gatcatggag
     3121 ggcaccccct cggaggtcag gaaggaccgg cgcgtcatag aggcttacct gggagaggag
     3181 cccgtatgac aggacattca aaccaaaacg ggcccgcact tctttcggtc cgcggcctct
     3241 gcgtctccta cggtataatc ggagccctcg atgaggtgaa catcgaggtg cgccggggcg
     3301 agatactgtc catcatcggg gccaac
//
LOCUS       DAOPOA010000177         3317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_31294_length_3317_cov_2.738811, whole genome shotgun sequence.
ACCESSION   DAOPOA010000177 DAOPOA010000000
VERSION     DAOPOA010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31294_length_3317_cov_2.738811"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="PKJ17_04780"
     CDS             <1..1532
                     /locus_tag="PKJ17_04780"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3488 and DUF4129 domain-containing
                     transglutaminase family protein"
                     /protein_id="HOD75317.1"
                     /translation="FYSQDAEMELTNSTVRTIIARCLWIPLLAIPLSAAMFVILPRTS
                     YPILTFLNRPDKARAGFTDNVRLGQVSDIQEDERIIFRATMDRIDETSLYWRGITLNH
                     FDGVSWRGTRRGVYAPARTRVPVGKMISQTIYLEPYDNSSLFALDKPVHIELRRVRKN
                     DDHTFTMPRFIERRIRYTALSALSNVIPEENISLKTHLQLPPDLSPRIVSLAKSLAVR
                     GDRNATVNRILSHLASDRYGYSLKDLPVSKNPLESFLFDSPKGNCEYFASAMAVLLRV
                     NEIPSRLVGGYQGGYYNEMGNYYIVPQKNAHVWVEAHIPGKGWVRFDPTPAGAGLSSL
                     SGRGALSKLRMFLDTINYYWYGIILTYNLERQISITRTIVSGLRRPSSFSFSPLMKKT
                     AQYSALFAAAIGLILLAWFLISSAKRREMVMLDRFLKRLKTAGYEKQTSEGLEEFIRR
                     IGDDGLRTASMEFARAFEEIYYGDRRFTADDLKRLNHLVGKIDDVAGQEDRMADLTPG
                     R"
     gene            complement(1536..>3317)
                     /locus_tag="PKJ17_04785"
     CDS             complement(1536..>3317)
                     /locus_tag="PKJ17_04785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD75318.1"
                     /translation="GARLAIVTNAGGVGIIATDTLIELEGDVAELSPESVNRLDALLP
                     ADWSGGNPVDIVGDADLSRYTGSIQICMSDEGVDGVLVVYTPRAEVEPVELSEALCDL
                     SRRSKKPLIAVWMAGVVAEKGRDLLIENNIPVYRTPEEAVKAYLYMHQYRKNIEMLYE
                     IPEDISPGHSRLNNHQKAIIRNCLREGTNSLSMEQSVSLMKTYDIPFPPTSFPRGMDE
                     AVREAEAMGFPVTLKVAGGESAGPGELFPGIASRQTLLEVLGGIRERSSRRPDPTDMP
                     EMMIQKTVRGKTAGWFLNTRRDIDFGSTIFLSAAREDGPSGDRSVVALPPLNQTLAKI
                     FLEDAGVQEALRKEDRQGLQSLLRQFEALCVNFACLIVDFPEILTIEAGPIVVDDGGV
                     YMTDAVIDLDGGFSRQPARYYSHLVITPYPTQYVTPWTLRDGTDIVMRPIRPEDEPLS
                     REMLARTSEEALRVRFFSSVNVQEIPHSTLMKFCNVDYDREVALVAEVREAEEKRIVG
                     GGRLIIEPDLKRAQFAVLVHDGLHGKGLGEKFVDMLIGIAQERGLEGIYGIVLTENEK
                     MLRLCKKMGFVSKRLPDGITRVEMKLN"
BASE COUNT          695 a         1028 c          907 g          687 t
ORIGIN      
        1 ccttctattc gcaggacgcg gagatggaac tcacgaacag cacggtgcgg acaatcatcg
       61 cgaggtgcct gtggataccg ctgcttgcca ttcccctttc cgccgcgatg ttcgtcatcc
      121 ttccccgcac cagctatccc atcctcacct ttctcaacag gcccgacaaa gcgagggctg
      181 gcttcacgga caacgtcagg ctgggccagg tatccgatat ccaggaggac gaaaggatca
      241 ttttcagggc caccatggac aggatagatg aaaccagcct ctactggagg ggtatcaccc
      301 tcaaccactt cgacggggta tcctggagag gcaccaggcg tggcgtctac gcgccggcca
      361 ggacccgggt gcccgtcggc aagatgataa gccagacgat atatctcgaa ccttacgaca
      421 attcctccct ctttgccctc gacaaaccgg tccacatcga gctcagaagg gtaaggaaga
      481 acgacgacca cacattcacc atgccgcgct tcatagagag gcggatccgt tataccgccc
      541 tgtcggctct ctcgaacgtc atacccgagg agaacatctc cctgaagacc cacctccagc
      601 ttccccccga cctctcgccg cgcatcgtct ccctcgcgaa gagccttgcc gttcggggtg
      661 acaggaacgc caccgtgaat cgcatcctga gccacctcgc ctccgacagg tacggctatt
      721 ccctcaaaga ccttcccgtc tcgaagaacc ctctcgaaag ctttctcttc gattccccaa
      781 agggaaactg cgagtacttc gcttcggcaa tggccgttct cctgcgtgtc aacgagatcc
      841 cttcccgcct tgttggaggg tatcagggcg gatactacaa tgaaatgggc aattactata
      901 ttgtgcccca gaagaatgcc cacgtgtggg tggaggcaca catacccgga aaggggtggg
      961 tccgattcga tccgacacca gccggggcgg ggctttcgtc cctctcgggg aggggggccc
     1021 tgtcgaagct ccggatgttc ctcgacacca taaactacta ctggtacggg atcatcctca
     1081 cctacaacct tgaaaggcag atatccataa cgaggaccat cgtgtcgggc cttagaaggc
     1141 cgtcgtcctt ctccttttct cccctcatga agaagacggc gcaatattcg gcgctctttg
     1201 cggcagccat cggcctcatt ctcctggcct ggttcctcat ctcctcggcg aagcgcaggg
     1261 agatggtgat gcttgaccgt ttcctcaagc ggctcaagac agcgggatac gaaaaacaga
     1321 catcggaggg cctcgaggaa ttcatccggc gcatcggcga tgacggcctc aggacggcct
     1381 ccatggaatt tgcccgggct ttcgaggaga tctattacgg ggacaggcgg ttcacggcgg
     1441 atgatctcaa gagactgaac catcttgtcg gcaagatcga cgatgttgca ggacaggagg
     1501 acaggatggc cgatctgacg ccggggcggt aagggtcaat tcaatttcat ctccacccgc
     1561 gtgatcccat cgggcagccg ctttgataca aagcccattt tcttgcagag ccggagcatc
     1621 ttctcgttct cggtgagaac tatcccgtaa atgccctcaa gccccctctc ctgggcgatc
     1681 cctatgagca tatcgacgaa cttctcgccc agacctttgc cgtggagacc gtcatggacg
     1741 aggaccgcga attgcgccct cttgagatcg ggctctatga tgagccgtcc gcctccgaca
     1801 atgcgctttt cctcggcctc cctgacctcc gccaccaggg caacctcccg gtcgtaatcg
     1861 acattgcaga acttcataag ggtgctgtgc ggtatttcct gaacgttgac ggaagagaag
     1921 aaccgcaccc tcaaggcctc ctcggaggtc cgggcaagca tctcacgact cagaggctcg
     1981 tcctcggggc gtattgggcg catcacgatg tccgttccgt ccctcaatgt ccagggcgtc
     2041 acgtactggg tgggataggg ggttatgacg agatgggaat agtagcgcgc aggttgcctc
     2101 gagaacccgc cgtcaagatc gatcactgcg tcagtcatat agacgccgcc gtcatcgacc
     2161 acgatgggac cggcctcgat cgtgaggatc tccggaaaat cgacgatgag gcacgcgaaa
     2221 ttgacacata gggcctcgaa ttgtctcaac agcgactgga gcccttgccg gtcctctttc
     2281 cgaagggctt cctgcactcc cgcgtcctcc aggaatatct ttgcaagcgt ctggttcagg
     2341 ggaggcaagg ccacgacgga cctatcgccg gacggaccat cctcgcgagc ggctgagaga
     2401 aagatggtgg acccgaagtc aatgtccctc ctggtgttca ggaaccatcc cgccgtcttc
     2461 ccacgaaccg ttttctggat catcatttcc ggcatgtccg tcgggtccgg tcttcggctc
     2521 gatctctccc tgatcccgcc caggacctcg agaagggtct gccgggaagc aataccggga
     2581 aacaattcgc ccggtcccgc gctttccccg cccgcgacct tgagcgttac gggaaaaccc
     2641 atcgcttccg cctcgcgaac agcttcgtcc atacccctgg ggaatgacgt cggcggaaaa
     2701 ggaatatcat aggtcttcat cagagaaacg gactgctcca tggaaaggga gttggtccct
     2761 tccctgagac agttccggat aatggccttc tggtggttgt tcaaccgtga atggccgggc
     2821 gagatgtctt cgggtatctc gtagagcatt tctatgttct tccggtactg gtgcatgtat
     2881 agataggcct tgaccgcctc ttcgggggtc cggtaaacgg gtatgttgtt ctcgatgagc
     2941 aggtcccttc ccttttcagc cacgacgccc gccatccata ctgcgatcaa tggctttttg
     3001 gaccggcggg acaggtcgca caaagcttcc gaaagttcca cgggctcgac ttccgcgcgg
     3061 ggggtgtaga ccacgagcac accatcgacc ccctcatcgg acatgcagat ctgtatggaa
     3121 cccgtatacc gggaaaggtc ggcatcgccc acgatgtcga cagggtttcc cccgctccaa
     3181 tccgcaggca gcagggcgtc gagcctgttc acgctctccg gggacaattc cgcgacatct
     3241 ccctcgagtt ctataagggt gtcggtagca atgatgccga caccccccgc attggtgaca
     3301 atggcaaggc gcgcacc
//
LOCUS       DAOPOA010000178         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_31310_length_3315_cov_3.072086, whole genome shotgun sequence.
ACCESSION   DAOPOA010000178 DAOPOA010000000
VERSION     DAOPOA010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31310_length_3315_cov_3.072086"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKJ17_04790"
     CDS             <1..495
                     /locus_tag="PKJ17_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75319.1"
                     /translation="GFPGGLLVGHPGNPPGRHDDAERKKPDRKGCRGGNRAFFASESP
                     KRVLFGVFCAQECRKERHDLLPGCGDIAVHDHPDLIRVHAEVCVNQNIPKTYHLFPGN
                     AGRNASKVIRETGGCFPDDLEMMHHPDLHQFVLKKRIPAPDPYRSSLRSLPGCRGAAR
                     SRPS"
     gene            complement(490..783)
                     /locus_tag="PKJ17_04795"
     CDS             complement(490..783)
                     /locus_tag="PKJ17_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOD75320.1"
                     /translation="MSVTHDLIQKKRSRILAIAGRHGATNLRIFGSVARGEAGPESDL
                     DLLVDLEPGRSLLDHIALIQDLEEALGCRVDVVTETALKERYKKRILKEAVPL"
     gene            complement(904..1149)
                     /locus_tag="PKJ17_04800"
     CDS             complement(904..1149)
                     /locus_tag="PKJ17_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HOD75321.1"
                     /translation="MIPERCTFCKGTLQEGKTEFIARVGDGIIVIRDVPALVCDRCGE
                     AYYSTEVSRKIDAVMADAHSGRLCCRPLAAGEVELTG"
     gene            complement(1146..1460)
                     /locus_tag="PKJ17_04805"
     CDS             complement(1146..1460)
                     /locus_tag="PKJ17_04805"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HOD75322.1"
                     /translation="MNRGRDEIAELVDRDSVIISHHARVRMFERNISTDALLSVIRVG
                     EIIESYPDDEPCPSVLILGFIRADAYHVVLGICDDHLRVITAYMPDVEDWIDTRTRRG
                     RE"
     gene            1899..2210
                     /locus_tag="PKJ17_04810"
     CDS             1899..2210
                     /locus_tag="PKJ17_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75323.1"
                     /translation="MEFFDDDAGKRIGTASLQSPSIAAYEANAAEVLANTALATAFAG
                     YAERLFENETYYCQLKCHDPSGDDYYVTFTNRAVQISSYQDDAIKDAVETWADAVGAL
                     E"
     gene            complement(2289..2663)
                     /locus_tag="PKJ17_04815"
     CDS             complement(2289..2663)
                     /locus_tag="PKJ17_04815"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HOD75324.1"
                     /translation="MGGGRPGAIEIREARKSGWLRVVEPSDKALVRLLEQELHAGEAE
                     SIALALEIRPDVLLLDETEARRVAGLYGLPVTDIIGLLIQARREGRVSSLAEEMDRLR
                     EEGGFWIQDALYRRVLEMENDE"
     gene            complement(2768..3028)
                     /locus_tag="PKJ17_04820"
     CDS             complement(2768..3028)
                     /locus_tag="PKJ17_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HOD75325.1"
                     /translation="MSDVTIKVPRDIVQALRLPPDVVAVELQRELAVALYQRGILSSG
                     KAAALAGMNRWEWEELLGARKISRHYAEEDLDQDIGYADCGE"
BASE COUNT          699 a         1054 c          908 g          654 t
ORIGIN      
        1 gggttcccgg gtggtcttct cgttggtcat cctggtaacc ctcccgggag acacgacgac
       61 gcagaaagga aaaagcctga ccgtaaaggt tgtcgcgggg gaaatcgcgc ttttttcgct
      121 tcagaaagtc caaaaagggt gttgttcggc gtcttctgcg cccaggagtg tagaaaggag
      181 cgccacgacc tcctccctgg gtgcggggat atcgcggtcc acgaccaccc agatctcatc
      241 agggtccacg cggaagtatg cgtgaaccag aacattccga agacctatca cctgtttcca
      301 gggaacgccg ggagaaacgc atcaaaggtc atcagggaga cgggcggctg cttccccgat
      361 gatctggaga tgatgcacca tccagacctg caccagttcg tcctgaaaaa acgcatccct
      421 gccccggatc catatcgctc atctcttcga tcgcttccag gatgtcgagg agccgctcgt
      481 tcccgtccct cataacggca ctgcctcttt gagaatcctc tttttgtagc gttctttgag
      541 cgcagtctcc gtcaccacgt ccacccggca cccgagagcc tcctccaggt cctggatgag
      601 tgcgatgtgg tcgaggaggc tcctgcccgg ctccagatcc accaggagat ccaggtcgct
      661 ctccggcccc gcctcgccgc gggcgacaga cccgaagatc cgcaggttcg tggcgccgtg
      721 ccgtccggct attgcgagaa tcctggaacg cttcttctgg atcagatcgt gcgtgacgga
      781 catggctatt ccaatcagtc ttatcggaaa ctggagatat aaatttgccc ctggtgaccg
      841 catcgctaaa attgcggcat agagggcagc cttcacgacg ggaacggccg tccgcccctt
      901 tccttatccg gtgagttcaa cctcgcccgc cgcgagaggc cggcagcaga gcctgccgct
      961 gtgggcatcc gccatcacgg cgtcgatctt ccttgaaacc tctgtgctgt agtatgcctc
     1021 gccgcaccgg tcgcagacga gcgccgggac atccctgatg acgatgatcc cgtccccgac
     1081 ccgtgcgatg aactcggtct tcccttcctg cagggttcct ttacagaacg tacatctttc
     1141 tggaatcatt ctcttcccct cctcgttcgc gtatcgatcc aatcttcaac gtctggcatg
     1201 taagcggtga tgacccggag atgatcgtca cagatcccga gtactacatg gtatgcatcg
     1261 gcacggataa agccgagtat gagcactgag gggcagggct catcgtcggg atatgattcg
     1321 atgatctccc ccaccctgat cactgagagg agagcgtcgg tggagatgtt acgctcgaac
     1381 atcctgaccc gggcgtggtg cgaaataatc actgaatccc gatccaccag ttcggcgatc
     1441 tcatccctgc cgcgattcat cttttccctt ccttaacagt tctatcaccg atgcactaaa
     1501 acatttccag ccggcagccc gtcacggaaa aggccccctt cgcttattat acggcgcctt
     1561 tcccgtgcaa tctaccccgc accgcccccg ataaagtcag gctaatcccc ccgtccaccc
     1621 aatagttgcc tgtcatagtc ccgggaggat gcaggccatc aggcacaacc aggaagcgtt
     1681 gtgcctcagg agaaaaaacc acggcagact tcgtgcagaa gaccgtaaac aaaacgacga
     1741 tccggaattt tgccgtcccg atcgccaccg taacgccttt aacaacctca tcgagatcgt
     1801 catcgacgac aacccctccg ggtgcgttgg gtataccggc tccgacgggg tccccgttcc
     1861 gggggtcatc cggaaccgcg agtactacac cttgatgagt ggagttcttc gacgacgacg
     1921 ccggcaaacg catcgggacc gcctccctgc agtccccctc gatcgccgca tatgaggcga
     1981 acgccgcgga ggtcctcgcg aacaccgccc tcgcaaccgc gtttgccggc tatgccgagc
     2041 gcctcttcga gaacgagacc tactactgtc agctcaagtg ccacgacccg tccggtgacg
     2101 actactatgt caccttcacc aaccgggccg tccagatctc gtcctaccag gacgacgcca
     2161 tcaaggacgc ggtcgagacc tgggccgacg ccgtcggcgc gctggagtga gggggctctt
     2221 gtcctcttta tcgttccacc tacatgcgac ggaactgact ggccccattc ctgatcgaaa
     2281 atttcggctt actcgtcatt ctccatttca agaacgcgcc ggtagagggc atcctgaatc
     2341 cagaagccgc cctcttcccg taaccggtcc atctcctctg caagtgaaga aacccgccct
     2401 tcacgcctcg cttgaatcaa tagtccgata atgtcggtga ctggaagacc gtagagcccc
     2461 gctacgcggc gggcctcggt ctcatcgagg agcagcacat cgggccggat ctcaagagca
     2521 agggcaatac tctctgcttc gcccgcgtgg agttcctgct caagcaggcg caccagagcc
     2581 ttgtcggagg gctcgaccac acgcagccac cccgacttac gggcttcccg gatctcgatg
     2641 gcccccgggc ggccgccgcc caactctacg acctcctgcc agaccgcggg agggatgcac
     2701 actgatgaaa agcggcgaag aaggtcgata tgcccgacca tcgagaggtg aatcagcggc
     2761 gaagaattta ctctccgcaa tcggcatagc caatgtcctg gtcgaggtct tcttcggcat
     2821 agtgccggga gatcttccgt gctccgagta attcctccca ctcccaccgg ttcatccctg
     2881 caagagccgc cgccttcccg gaagagagga tgccccgctg gtagagcgcc acggcaagct
     2941 cccgttgcaa ctcgacggca acgacatcgg gagggagccg aagtgcctgg acgatatctc
     3001 ggggaacctt gatggtgaca tcggacatag ctggtttcac ctgatgaata aaagataggg
     3061 gggagaaccg ataaacgtgg cggtgagttg aggcgccgca gaattacctc tcggtcagaa
     3121 tgttctattg ccccggaaag caggattctt cgatcccata cccgaggagc ggacgcttcg
     3181 gaagatcatc gtccgacaga catgaaacca ccactatatg atcagcgtcg agtggggggt
     3241 ggggatgacc ccggagccgt gggtgtcgcc gtagaccgag gaggatgagg cggcgacgac
     3301 ggcgggcacc ccgca
//
LOCUS       DAOPOA010000179         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_31445_length_3304_cov_2.663281, whole genome shotgun sequence.
ACCESSION   DAOPOA010000179 DAOPOA010000000
VERSION     DAOPOA010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31445_length_3304_cov_2.663281"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PKJ17_04825"
     CDS             <1..432
                     /locus_tag="PKJ17_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOD75326.1"
                     /translation="LFWIVTYVIVGGFTTHYIISLKDIRSIPGQLSYYIHGLFQGSTN
                     PFVPSPESRFNALQKLAYSFIMIVAMPIIIITGIIFGNIMDFYGIIRSTGGIRVLDAI
                     HVIVGYVFLIYLIVHLYMATLGRTPFSHTKAMFTGHEEEFK"
     gene            547..1491
                     /locus_tag="PKJ17_04830"
     CDS             547..1491
                     /locus_tag="PKJ17_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOD75327.1"
                     /translation="MGLAGIDGSGVKLIMVGGKGGVGKTTCASAIALKMAEEGRKVLI
                     ITSDPAPSLSDIFEKEIGDRETRIDDALELYGLEVSSDIVLARWKERFGPEIYEVVSS
                     FASVDYDFVEYIGTAPGIEEEYMLNFIMELVEGGSYDVVVWDTAPAGHTLRLLRLPQL
                     FLSHMEAATKFYMNMYGYIEKVKDTIRLKESKKTLLQIIAGWEALSERIVEFIRDGSC
                     VKYLIVTIPEALGVKMTNRVIGELEENRLTVENIVINNVVREADCEFHRLRKTMQEHY
                     IEELRETYRGKTLTLLYLAAQEIKGMDRIAGVTRSLFG"
     gene            1497..2072
                     /gene="rsmD"
                     /locus_tag="PKJ17_04835"
     CDS             1497..2072
                     /gene="rsmD"
                     /locus_tag="PKJ17_04835"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOD75328.1"
                     /translation="MATLRITGGRLKGMSIRVLPGDVARYTSSKVRQALFNMIGSVDG
                     LSVLDLFAGSGSFSIEALSRGAASATLVEANRRVTDLIGKNLARTGLNKYCQVLHMDV
                     RYAVPLLYGRKCMYDVIFMDPPYEMGYVTRTMTLLDEHPLRNSDTITIAEYSRRESDC
                     LSLFEGLKGARTRKYGDTMISLFRDPLNPGA"
     gene            2082..2570
                     /gene="coaD"
                     /locus_tag="PKJ17_04840"
     CDS             2082..2570
                     /gene="coaD"
                     /locus_tag="PKJ17_04840"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOD75329.1"
                     /translation="MKETLAVYPGSFDPVTFGHLDILARGLELFDKVIIAIAYNIEKQ
                     GLFTFEERKDLIQQSINGNNNVIIDSFDGLLIDYVKKVNARFVIRGLRAMSDFEYEFQ
                     MASINRTLNPGMDTLFMMTSKDYFFISSRTIKEVAAFHGSVSSFVPAPVERRLKEIFE
                     KR"
     gene            2567..>3304
                     /locus_tag="PKJ17_04845"
     CDS             2567..>3304
                     /locus_tag="PKJ17_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75330.1"
                     /translation="MKRLCFIIALIGAVVLFAPFSHAKDVYTVRIQGAINPAVAGFLG
                     ECIEKAGKADAEALVVLLDTPGGLDSSMRDIVKEIMDAPVPVIVYVAPSGARAASAGA
                     IILLASHVAAMAPGTNAGAAHPVSIGKEKTDEVMMKKVVSDAEAYSKSIAARRGRNVD
                     FAAKAVTESISITARDALAQKVIDVVADSTDDLLVQMDGRTVETRKGKVVLRTKGAKK
                     TEIAMPFKFRFLAYVSDPNVAYILMMIG"
BASE COUNT          765 a          904 c          935 g          700 t
ORIGIN      
        1 ctgttctgga tagttacata cgttatcgtg ggcggcttca cgacccatta tatcatttct
       61 ctcaaggaca tacggtccat ccccggtcag ctgtcctact acatccacgg tctttttcag
      121 ggcagcacga accctttcgt gccctccccg gaatccaggt tcaacgcctt gcagaaactt
      181 gcctattcct tcatcatgat cgtcgcgatg ccgatcatca tcatcacggg gataatcttc
      241 ggcaacataa tggatttcta cggcatcatc cgctccacgg gcggtatccg cgtcctcgat
      301 gccatccacg tgatcgttgg atatgtcttc ctgatctatc tcattgtcca cctctacatg
      361 gcaaccctgg gcaggacacc cttctcccac accaaggcga tgttcacggg ccacgaggaa
      421 gaatttaagt gaaagaaatt atgacgtttt tgcctaaaac ctaaaacgta aaacctaaaa
      481 cgtaaaacct gtttcctgtc cgtcttccct gtctttcccg cgtcaaagat ggtatgatgt
      541 tcgtctatgg gacttgcagg catcgatgga tccggggtca agctcatcat ggtcggcggc
      601 aagggtggcg tgggcaagac gacgtgcgcg tcggcgatcg ctctgaagat ggccgaggag
      661 gggcgaaagg tccttatcat cacgagcgat ccggccccct ccctttcgga tatcttcgag
      721 aaagagatcg gtgaccggga gacccggata gatgatgctc tcgaactcta cggtctggag
      781 gtctcctccg atatcgtcct ggcgcgctgg aaagagcgtt ttggaccgga gatctacgag
      841 gtcgtctctt cctttgccag cgtcgattac gatttcgtcg agtacatagg aaccgccccg
      901 gggatagaag aggaatacat gctgaatttc atcatggagc tcgtggaagg cggcagctac
      961 gacgtggtcg tgtgggacac ggcccccgcg gggcataccc tgaggctcct caggctgccc
     1021 cagctcttcc tgtcgcacat ggaggcggcg acgaagttct acatgaacat gtacggatac
     1081 atcgagaagg tgaaggacac gatacggctc aaggaatcga agaagacgct cctccagata
     1141 attgcgggct gggaggccct ttcagagcgc atcgtggagt tcatcaggga cggttcatgc
     1201 gtgaagtacc tcatcgtcac catccccgag gccctgggcg tgaagatgac gaaccgggtc
     1261 atcggcgagc tcgaggagaa ccgtctcacc gttgagaata tcgtcatcaa caacgtggtg
     1321 cgggaggccg actgcgagtt ccaccgcctc cgcaagacca tgcaggaaca ctatatcgaa
     1381 gagctgagag agacgtacag gggcaagacc ctgaccctcc tgtacctcgc cgcccaggag
     1441 ataaaaggca tggacagaat agcgggcgtg accaggtcgc tcttcgggta ggacccatgg
     1501 caacgctgcg cataacggga ggaaggctca agggcatgtc gatccgtgtc ctgccgggtg
     1561 acgtggcccg gtatacctct tccaaggtga ggcaggctct tttcaacatg atcggaagcg
     1621 tggacggcct gtccgtcctc gatcttttcg ccggttccgg ttccttctcc atagaggcac
     1681 tgagccgggg tgcggcatcg gcgacactcg tcgaggcgaa caggagggtg acggacctta
     1741 tcgggaagaa cctcgccagg acgggattaa ataagtattg ccaagtgtta catatggatg
     1801 taagatatgc agtccctttg ctctatggca ggaagtgcat gtacgatgtg atcttcatgg
     1861 accctcctta cgaaatggga tatgtgacgc gcacaatgac acttcttgat gaacatccac
     1921 tccggaattc cgatacaatc accatagccg agtactcaag gcgcgaaagc gactgtctgt
     1981 ccctcttcga ggggctcaaa ggcgcaagga cgaggaaata cggcgacacg atgatctcgc
     2041 tgttcaggga cccactaaat cccggtgctt aaggagaaac catgaaagag acgctcgcag
     2101 tgtatcccgg ttcattcgac cccgtgacct tcggccatct cgacatcctc gcgagggggc
     2161 ttgaactttt cgacaaggtc ataatcgcca tcgcctacaa tatcgaaaag caggggctct
     2221 ttacatttga agagcggaag gacctcatcc agcagtccat aaacgggaac aacaatgtca
     2281 ttatcgatag tttcgacggt ctgctcatcg actacgtgaa aaaggtgaat gcccgtttcg
     2341 tcatcagggg cctgcgcgcc atgagtgact ttgagtacga gtttcagatg gcctccatca
     2401 acaggaccct caatcccggg atggataccc tcttcatgat gaccagcaag gattatttct
     2461 tcatcagttc gagaacgatc aaggaagtcg ccgcattcca tggctccgtc tccagtttcg
     2521 tgcccgctcc cgtcgaaagg cgactcaagg agatctttga aaagcgatga agagactgtg
     2581 cttcattatt gcgcttatcg gcgcagtcgt tcttttcgca ccattctccc acgcaaaaga
     2641 tgtgtacacg gtccgcatac agggcgccat caatccggcc gtggcgggct tcctggggga
     2701 atgtattgag aaggccggca aggcggatgc cgaggccctt gtggtcctcc tcgatacgcc
     2761 ggggggattg gattcgtcca tgcgggacat cgtgaaggag atcatggacg cgccggttcc
     2821 cgtcatcgtc tatgtcgccc catcgggggc gagggcggcc tcggcgggcg ccatcatcct
     2881 tctggcctcc cacgtggcgg cgatggcccc cgggaccaac gcgggcgccg ctcaccctgt
     2941 cagcatcggc aaggaaaaga ccgacgaagt gatgatgaaa aaggtggtca gcgacgcgga
     3001 agcctattcg aagagcattg ccgccaggcg gggaagaaac gttgatttcg ctgcgaaggc
     3061 ggtcacggaa agcatctcga taacggcgag ggatgccctg gcgcaaaagg tcatcgatgt
     3121 cgtggccgat tccacagacg acctcctggt acagatggac ggcaggaccg tggagacaag
     3181 gaaaggaaag gtcgtactgc gcacgaaggg tgcgaagaag acagagatcg caatgccctt
     3241 caagttccgc ttcctcgcct acgtgagcga tcccaacgtg gcgtacatac tcatgatgat
     3301 cggg
//
LOCUS       DAOPOA010000180         3297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_31527_length_3297_cov_3.116595, whole genome shotgun sequence.
ACCESSION   DAOPOA010000180 DAOPOA010000000
VERSION     DAOPOA010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31527_length_3297_cov_3.116595"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PKJ17_04850"
     CDS             <1..543
                     /locus_tag="PKJ17_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOD75331.1"
                     /translation="LLTSLTIAREWERGTMEQLVSTPVKPAELVVGKLAPYFAIGIID
                     TVMSIVIVVALFGVPLKGSVLLLLFISSLFLFGGLSLGILLSIVTRSQLAASQVAVIT
                     SYLPALLLSGFMFSIVNMPVPLQAITYLIPARYFVSAIKGIFLKANTAGMLLAEMGLL
                     ALFGAIVFTLALKRFKKKVA"
     gene            545..1666
                     /locus_tag="PKJ17_04855"
     CDS             545..1666
                     /locus_tag="PKJ17_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD75332.1"
                     /translation="MRDTLRRIKHMVVKEFIQIFRDRKMKPIIFLTPVLQLIVFGYAV
                     TMDVTNIRMAVHDLDKTSVTREIARRFEASGYFRIVRFAASEQEVRDLLDRGKATVVL
                     NFDRGFTSDLEARRGASVQILIDGADSNTALVAAGYAGAIISRYGRDIATRMASWTPG
                     SLDVRPRAWYNADLRSRNYNVPGVIAIMVLLVCLLLTSMAIVREREIGTMEQLMVTPI
                     RPSELILGKTIPFAVIGFFDMFLVTVVGVAWFDVPIRGSIPLLVLATAVYLLSVLGFG
                     LFISTISRTQQQAMMATFLFFAPAILLSGLMFPIENMPRAVQFITYLNPLRYFLVIIR
                     GIFLKGSGMAILWPQFLALFLLGASVVATSSLRFKKRLG"
     gene            2163..2945
                     /locus_tag="PKJ17_04860"
     CDS             2163..2945
                     /locus_tag="PKJ17_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75333.1"
                     /translation="MYDTKPFNPEADRLPPMYTGHNPELLYNGIEMRKQRAMRGTGET
                     EEQHRARMAREIALPLMGFLDFDSVYAVRVTPAEVRRDGKDGLIHVSCRLCAAFEAGR
                     EDPGKKAFVVRYLPHLDNRYTIARKDGTRVTIEEIKFSEYAVVIANARDLPIQRTVGP
                     QAREDGRRQKEGDEKPEGERIEEAIELTADQARRMEGGIMALLIGKLAPPYTSHEEIG
                     RKPTAERPGTYLGRYHYLYMDLMTIWFYDVTTGKVLKKMRFS"
     gene            complement(2974..>3297)
                     /locus_tag="PKJ17_04865"
     CDS             complement(2974..>3297)
                     /locus_tag="PKJ17_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75334.1"
                     /translation="RDGEEWLTQCLAYLESNRGLLEHFLGERSPSLSMSRMEATYLAW
                     IDCRESRIPGNPFRFFLEEGRVALNDGREYGKGGEGFVRLNFACPRGSLTEALERMAD
                     AMQRL"
BASE COUNT          651 a         1034 c          952 g          660 t
ORIGIN      
        1 ctgctcacct ccctcaccat agccagggaa tgggagcgtg ggacgatgga acagctcgtc
       61 tcgacgcccg tgaaaccggc ggaactcgtc gtcgggaagc tggcccccta cttcgccatc
      121 ggcatcatcg acaccgtcat gtccatcgtg atcgtcgtgg cgctcttcgg ggtgcccctc
      181 aaggggagtg tccttctcct cctgttcatc tcgtccctct tcctcttcgg gggactcagc
      241 ctgggtatcc tcctgtcgat cgtgacgaga tcgcagctgg cggcaagcca ggtcgccgtc
      301 atcacgtcct atctgccggc gctcctgctg tcgggtttca tgttctccat tgtgaacatg
      361 cccgtgcccc tgcaggccat cacctatctc atacccgccc ggtacttcgt ctccgccatc
      421 aaggggatat tcctcaaggc aaacaccgcc gggatgcttc tcgccgagat ggggctcctt
      481 gcgcttttcg gcgctatcgt cttcaccctg gcgctgaaaa ggttcaagaa aaaggtggcc
      541 tgacatgaga gacacgctcc ggcgcatcaa gcacatggtc gtcaaggaat tcatccagat
      601 attccgtgac aggaagatga agcccatcat cttcctgacc cccgtccttc agctcatcgt
      661 cttcggctat gccgtgacga tggatgtcac caatatccgc atggccgtcc atgacctcga
      721 caagacgtcg gtcacccgcg agatcgcccg gcgtttcgag gcgtcgggat acttcaggat
      781 cgtgcgcttc gccgcgtccg aacaggaggt aagggacctc ctggaccgcg gaaaggccac
      841 agtggtcctc aatttcgacc ggggcttcac gtcggacctc gaggcacgtc gcggggcctc
      901 cgtccagata ctcatcgacg gcgcggactc gaacaccgcc cttgtcgcgg caggatacgc
      961 cggcgccatc atctcccggt acggccggga catcgccaca cggatggcct catggacgcc
     1021 gggctccctg gacgtgcgcc cccgcgcctg gtacaacgcg gacttgagga gcaggaacta
     1081 caacgtgccg ggggtcatcg caatcatggt cctcctcgtg tgccttttgc tcacatccat
     1141 ggccatcgtc cgggaacggg agatcggcac catggaacag ctcatggtca cgcccatccg
     1201 cccctccgag ctcattctcg gaaaaaccat ccccttcgcc gtcatcgggt tcttcgacat
     1261 gttccttgtc accgtcgtcg gcgtcgcctg gttcgacgtg cccatacggg gctccattcc
     1321 ccttctcgtc ctcgcgactg ccgtgtacct cctttccgtc ctcggcttcg gcctcttcat
     1381 ctccaccatc tcccggaccc agcagcaggc gatgatggcg acgttcctct tcttcgctcc
     1441 ggcgatcctc ctgtcgggcc tcatgttccc catagaaaac atgccccggg ccgtccagtt
     1501 catcacctac ctcaatccac ttcggtattt cctcgtcatc atccgtggta tcttcctgaa
     1561 gggcagcggc atggccatcc tctggcccca gtttctcgcc ctcttccttc tcggggcatc
     1621 cgtcgtcgcc acgagttcgc tgaggttcaa gaagcggttg ggatgaaaga cggcttgaat
     1681 ggcttgaatg gcttgaatcg cttgacaggc tgttgacaaa gtgatttctg aagacgattc
     1741 gtgctctttc gtcattccgg cgaaggccgg aatccaggag aaacgagggg ttagagaaac
     1801 ctctggattt aaggccttcg ccggaatgac gtagaaaatg ggtttatcaa cagcctgttg
     1861 agggtttgca cattccagag gcaagctgaa gagtatccgg cggggtggac ggcgtttccc
     1921 cgacagtgtc ggctcgacgg ccgaaccgcc ttttggtgtt gacaatcggc gacaggcgat
     1981 accagaatga taggcaaaag gatgaaaggg agaatatgag atatcgccgg gggttcttca
     2041 cttccgttgc cgcattactg gcatgtagcg ttcttttctt ggccggttgt tcggggtcgg
     2101 gcaagggggg atccggcggg ggacatcggc cgggtgggcc gggaaacggc tttctctcca
     2161 cggtgtacga cacgaagcct ttcaacccgg aggcggacag gctccctccc atgtacacgg
     2221 gacacaaccc ggaactcctc tacaacggca ttgagatgag aaaacaaaga gcgatgcggg
     2281 gaacggggga gacagaggag cagcacaggg ccaggatggc ccgggagata gcccttcccc
     2341 tcatgggttt cctggatttc gacagcgtct atgccgtgag ggtcaccccc gccgaggtcc
     2401 gccgtgacgg gaaggacgga ctcatccacg tgagctgccg gctctgtgcc gcgttcgagg
     2461 cgggccggga ggaccctggg aaaaaagcct tcgtcgtcag gtacctgccc cacctcgaca
     2521 acaggtacac catagccagg aaggatggca cccgggtgac catcgaggag ataaagttca
     2581 gcgaatacgc ggtggtcatc gccaacgcga gggacctccc gatacagagg accgtagggc
     2641 cgcaggcacg cgaggacggc aggagacaga aggagggcga cgaaaagccg gagggcgaaa
     2701 ggatcgaaga ggcgatagag ttgacggccg accaggcacg acgcatggag ggcggtatca
     2761 tggcgcttct catcggcaag ctcgcgccgc cgtacacctc gcacgaggag atcggccgca
     2821 agccgacggc ggaaagacca ggcacctatc ttgggagata tcattacctg tacatggacc
     2881 tcatgacaat atggttctac gacgtgacaa cagggaaggt cctcaagaag atgcggttct
     2941 cttagccgtt ccgaccgccc cttctttcgg cgactacaac ctttgcatgg catcggccat
     3001 gcgctcgagc gcctcggtga ggctccctct cggacaggca aagttgagcc tgacaaaccc
     3061 ctctccgccc ttcccgtatt cccggccgtc gttcagggcc accctcccct cctcgagaaa
     3121 gaagcgaaac gggttccccg gtatccgcga ctccctgcaa tcgatccagg caagataggt
     3181 cgcctccatg cggctcatcg aaagggaagg cgatctctcg cccaggaagt gctccagaag
     3241 acccctgttg ctctcgagat aggcaagaca ttgggtgagc cactcctcgc cgtccct
//
LOCUS       DAOPOA010000181         3294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_31559_length_3294_cov_4.121334, whole genome shotgun sequence.
ACCESSION   DAOPOA010000181 DAOPOA010000000
VERSION     DAOPOA010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31559_length_3294_cov_4.121334"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKJ17_04870"
     CDS             <1..581
                     /locus_tag="PKJ17_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75335.1"
                     /translation="RIFDPFFTTKDLGIGSGLGLASAYGIIKNHQGAITVDSKPAKGT
                     RFTIYIPASQKPVKEEEPAAAEVVRGAGTVLLVEDQPAVAETAREMLEALGYDVHVAG
                     SGGEALELFARHRNGVSLVILDMIMPGLSGSETYDRLKEMDPGIKVILCSGYSIEGQA
                     ARILERGCNSFIQKPFTISELSEKLMEVLGRS"
     gene            complement(578..820)
                     /locus_tag="PKJ17_04875"
     CDS             complement(578..820)
                     /locus_tag="PKJ17_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75336.1"
                     /translation="MTTEEEKLVIRYADQAFRGTTIRQEYPVCECGKIFSEKNLCDAP
                     GVFFKSVDVFGKTYTLIEPVCPICKRKIPASFNILN"
     gene            1180..2592
                     /locus_tag="PKJ17_04880"
     CDS             1180..2592
                     /locus_tag="PKJ17_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOD75337.1"
                     /translation="MSGVFGIVSKKNCASNLLYGTDYHSHMGTEYGGMAVLGDRFHRS
                     IHDISKSQFKSKFFEEYKEMKGNSGIGVISDRDAQPLIIGSKFGTFAIVSSGIVENTN
                     ELAKELLDSGETFSEMSGGGINSVELIAKLITQGKTLIDGIRGVYERIEGSASILLLT
                     ENGVWAARDRLGRTPLVVGEKDGDYAVATEDCSFLNLGFRRVRFLDPGEIVSIRPSGL
                     QSELPGTDRNQICAFLWIYTGYPASSYEGINVEVVRERSGRFLAKGDKVDADLVAGVP
                     DSGVGHAIGYSMESGLPYRRPLVKYTPGYGRSYTPPSQEIRDLVATMKLIPVRDVIAG
                     NRIVLCEDSIVRGTQLKNFTVVKLWDSGAKEIHIRPACPPLMYHCKFALSTRSVDELA
                     ARRAINDIEGRRTDDITEYLDDTSEKYRKMVDWIAREINVTTLKFQRIDDMIEAIGLP
                     REKLCLHCWTGEGGSACCAR"
     gene            2611..>3294
                     /locus_tag="PKJ17_04885"
     CDS             2611..>3294
                     /locus_tag="PKJ17_04885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD75338.1"
                     /translation="MRGDELFRRLAVREPFTGMHPSVGAFFRDYLSGEKAVRFGDRFV
                     INTHFPPFPSGAFDNLAEHFGALGQLDNRRLYSVTFGVTNRCGYRCWHCYNAGRQQQD
                     MTLDEVRRIARQLSDLGSVMVTLSGGEPLLRKDLEEITGSFGDRSCLMLNTTGAGLTE
                     TRARSLKKAGLFAVGVSLDSLDPEEHDRKRGKKGAFRTALRALTLASETGLYPYIVTV
                     ASRELLAPER"
BASE COUNT          706 a          974 c          988 g          626 t
ORIGIN      
        1 cccgcatatt cgacccgttc ttcacgacca aggacctcgg tatagggtcc ggcctcggcc
       61 tggcatcggc gtacggtatc ataaagaacc accagggggc cataaccgtc gacagcaaac
      121 ccgccaaagg gacccggttc accatctaca taccggcatc acagaaaccc gtgaaagagg
      181 aagagcctgc cgccgccgag gtggtccggg gagcgggaac ggtgcttctc gtcgaagacc
      241 agccggcggt ggccgaaacg gcgcgggaga tgctcgaggc cctgggctat gacgtccacg
      301 tggcgggaag cggcggtgaa gccctggaac tcttcgccag gcacaggaac ggggtgagcc
      361 tcgtgatcct cgacatgatc atgcccgggc taagtggaag cgagacctat gacaggctga
      421 aagagatgga cccgggcatc aaggtgatcc tttgcagcgg gtacagtata gaaggacagg
      481 cggcaaggat actcgaaagg ggctgcaact ctttcatcca gaaacccttc accatctccg
      541 aactatcgga gaaactgatg gaagtcctgg gcaggagcta gttgaggatg ttgaagcttg
      601 ccgggatctt acgcttgcat atggggcaga cgggctcgat gagagtgtat gtcttcccga
      661 aaacgtcgac gctcttgaag aatactcccg gagcgtcgca gaggttcttt tcactgaaga
      721 tcttgccgca ttcacacacg gggtattcct gccgtatcgt cgttcccctg aatgcctgat
      781 cagcgtacct gatgaccaat ttttcttctt ccgtcgtcat ggctttaata taatctcctt
      841 atccctcttt ggcaaccttg cccctgatgc ttcggcattc tcgacactga ctcacgggcg
      901 ccgtcctaca gatatgttcg gtatggggga gggaaactta aagaagacag ttgcgggttg
      961 cgggaaaaaa gacggtgaaa gccggcgcac gtcaacacgg ctgacctgtc agggtctcaa
     1021 cgcttcgtcg tttcggcgag gggaggacgt aaaaggcctg ccgggatacg ggcgggatgg
     1081 tgtgcattcc tgtggaattt tccggggaat ccatatatca tatccttgcc atttaaacgc
     1141 gttatcgaca actgatcagg atgccaccag ggagggcgta tgagtggagt tttcggcata
     1201 gtatcgaaga agaattgcgc gagcaacctc ctctacggta ccgattatca ctcgcatatg
     1261 ggaacggaat atggggggat ggccgtactg ggagaccggt tccatcgcag cattcacgat
     1321 atcagcaagt cgcagttcaa gtcgaaattt ttcgaggaat acaaggaaat gaagggcaat
     1381 tcgggcatcg gggtcatcag cgaccgggac gcccagcccc ttatcatcgg ctccaagttc
     1441 ggcaccttcg ccatcgtttc ctcaggcatc gtggagaaca cgaacgagct cgccaaagag
     1501 cttctcgaca gcggcgagac attcagcgag atgtccggcg gcggcataaa ctccgtcgag
     1561 ctcatcgcga agctgatcac ccagggcaag accctcatcg acggtatcag gggcgtatac
     1621 gaacgcattg aaggttccgc ttccattctg ctcctcacgg aaaacggggt ctgggcggcc
     1681 agggaccgcc tgggcagaac gccgctcgtc gttggtgaga aggacggcga ctatgccgtc
     1741 gccaccgagg actgctcctt tctgaacctg gggttccgga gggtcaggtt cctcgacccc
     1801 ggagagatag tgagcatccg cccgtcgggt cttcagagtg agctcccggg cacggaccgg
     1861 aaccagatat gcgccttcct ctggatatac acgggatatc ccgcctccag ttacgaaggg
     1921 atcaacgtgg aggtggtccg ggaacgctcg ggaaggttcc tggcaaaggg cgacaaggtg
     1981 gatgccgatc tcgtcgccgg cgtccccgat tcgggcgtcg gccatgccat cggttactcc
     2041 atggagtccg ggctccccta ccgcaggccc ctggtcaagt acacaccggg gtatggcagg
     2101 agctacacgc caccctccca ggagatacgg gatctcgtgg ccaccatgaa gctcataccc
     2161 gtgcgcgacg tcatcgcggg caaccgcatc gtcctgtgcg aggactccat cgtgcggggc
     2221 acacagctca agaatttcac cgtcgtcaag ctctgggatt cgggcgcgaa ggagatccac
     2281 atccgcccgg cgtgcccgcc tctcatgtat cactgtaagt tcgccctctc gacccggtcc
     2341 gtcgacgagc tcgccgcgcg ccgcgccata aacgacatcg aaggcaggcg tacggacgat
     2401 atcaccgaat atctcgacga cacctcggaa aaataccgga agatggtcga ctggatcgca
     2461 cgggagataa acgtcaccac tctcaagttc cagaggatcg acgatatgat agaggccatc
     2521 ggcctgcccc gggagaagct ctgcctccac tgctggaccg gggaaggcgg ctcggcctgc
     2581 tgcgcgcgat aacgcttcac acgaggtgat atgaggggag acgaactctt tcggcggctt
     2641 gccgtgcggg aacccttcac cggcatgcat ccgtccgtgg gggccttctt ccgggactac
     2701 ctttccggtg agaaggcggt ccgcttcggg gaccgcttcg tcataaacac ccactttcct
     2761 ccctttccga gcggcgcctt cgacaacctc gccgaacact tcggcgcctt ggggcagctg
     2821 gacaaccgca gactctattc ggtcaccttc ggcgtcacca accgctgcgg gtatcgctgc
     2881 tggcattgct acaatgccgg cagacaacag caggacatga ccctcgacga agtgaggcgg
     2941 atcgcccgcc agctgtccga tctcggaagc gtgatggtca ccctctcagg gggtgagccg
     3001 ctcctgagga aggacctcga agagataacc ggttctttcg gcgacaggtc ctgcctcatg
     3061 ctcaacacga ccggcgcggg actgacggaa acgcgtgcac gctccctgaa gaaggcgggg
     3121 ctttttgccg tcggcgtcag cctcgattcc ctcgaccccg aagagcatga ccgcaagcgt
     3181 ggcaagaaag gggcgttccg cacggccctt cgcgccctca cgctcgcgtc ggagaccggc
     3241 ctctatccct atatcgtcac ggtggccagc cgggagctgc tcgcaccgga gagg
//
LOCUS       DAOPOA010000182         3291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_31600_length_3291_cov_3.125773, whole genome shotgun sequence.
ACCESSION   DAOPOA010000182 DAOPOA010000000
VERSION     DAOPOA010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31600_length_3291_cov_3.125773"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKJ17_04890"
     CDS             <1..237
                     /locus_tag="PKJ17_04890"
                     /inference="COORDINATES: protein motif:HMM:NF021455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOD75339.1"
                     /translation="REGKTMSSGEFRDLVMMGRQPALVLFGTGWGLTEEVVELCDRML
                     EPISGAGDYNHLSLRVALGIILDRIFSERGGHHE"
     gene            230..577
                     /gene="rplS"
                     /locus_tag="PKJ17_04895"
     CDS             230..577
                     /gene="rplS"
                     /locus_tag="PKJ17_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOD75340.1"
                     /translation="MNEMVDLLEKEHMRLDLPEVGIGDNVKVYTRIFEGEKERIQVFE
                     GVVIRKRGGNTRASFTVRKVSYGVGVEKTFPMHSPLIDRIERTTRSKIRRSKLYYLRN
                     LRGKAAKLKEKRD"
     gene            588..1172
                     /locus_tag="PKJ17_04900"
     CDS             588..1172
                     /locus_tag="PKJ17_04900"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOD75341.1"
                     /translation="MICRLTGLVGGIDEAGRGPLAGPVVSACVIWERAPEDKNGVTDS
                     KLLTQKERVGLFSWIMDNALSVGIGMASREEIDEMNILKASLLSMERALLATGMEPDV
                     VLVDGNRGLRNYPNARPVVRGDRRCFFMASASIVAKVIRDRAMDAYHRIYPNYNFKQN
                     KGYPTREHKAAIREFGITPIHRVTFKGVREYCAG"
     gene            1162..1518
                     /locus_tag="PKJ17_04905"
     CDS             1162..1518
                     /locus_tag="PKJ17_04905"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOD75342.1"
                     /translation="MPDKLTTGREGEDRAAAVLIREGYRILDTNFRSPFGEIDIVAEE
                     SGCLVFVEVKRRRGRGFGTSFDAIDARKKTHIIRSAQVYLKKNRCVDRRIRFDVVGID
                     GDTVRVIRHAFGEEKG"
     gene            1709..2452
                     /gene="larB"
                     /locus_tag="PKJ17_04910"
     CDS             1709..2452
                     /gene="larB"
                     /locus_tag="PKJ17_04910"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOD75343.1"
                     /translation="MIDEKVMELLAAVRDGRISVEDAYERFRDVPFEDLEHTRVDHHR
                     VLRKGVPEVVFGEGKTLPQIYDIVGSFRQKDLDVLVTRIDAAIGEALTKRFPEGTYSG
                     DARCFWVRRERPAEGKGMVLVMSAGTSDAKVAEEAYVASTFFGNDTEKLYDVGVAGIH
                     RLFQNLPTLRRARVIIVAAGMEGALPSIVAGIVGVPVIAVPTSVGYGASFSGLTALLA
                     MLNSCSTVAVFNIDNGFGAAYFATLVNRL"
     gene            2449..>3291
                     /locus_tag="PKJ17_04915"
     CDS             2449..>3291
                     /locus_tag="PKJ17_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HOD75344.1"
                     /translation="MKILYIDPVFGISGDMMIGSLIDSGVPFDALEALVRKISPDAPS
                     MKPVRLTEGVISGIHLEIGQSDRFYTISEMEQVMGSIDVEKKVRDDALGMLSLIVAAE
                     SKIHGMSRDELHLHELSHIDTVIDLLCVAYGVRFLGADRVFCGPVPCGSGTITTSHGS
                     IPNPPPVTLEILRGHPLVFYGESLELTTPTGAAIVAHYTEPVGRVPAFRTIREGYGVG
                     TYKSSRPDVLRIFVGEADEPAYDHEVEVIEADIDDMEMEYMGAVADRLRAVGALDVLF
                     FPVSM"
BASE COUNT          699 a          902 c         1043 g          647 t
ORIGIN      
        1 agggagggta agacgatgtc ctccggcgaa ttccgcgacc tcgtcatgat gggacgtcag
       61 ccggccctgg tgctttttgg aacgggctgg ggcctgacgg aggaggtcgt ggagctttgc
      121 gacagaatgc ttgaacccat aagcggcgcg ggtgattaca atcatctttc ccttcgcgtc
      181 gcgctgggta ttatattgga tagaattttc agtgaaagag gaggacacca tgaatgagat
      241 ggtcgatctg cttgagaaag agcatatgag actcgacctc ccggaggtcg ggataggcga
      301 taacgttaag gtctacacga ggatcttcga gggagagaag gaaaggatcc aggtcttcga
      361 aggcgttgtc atccgcaaga gaggcgggaa cacacgggcg tcattcacgg tccggaaagt
      421 ctcctatggt gtcggcgtcg aaaagacgtt tcccatgcat tcgcccctca tcgacaggat
      481 cgaaaggacc acgaggagca agatcaggag atcgaaactg tactatctgc gcaacctcag
      541 ggggaaagcg gcgaagctca aagagaaaag ggattagcag aaggacgatg atctgccggc
      601 tgacaggtct cgtgggtggc atcgatgagg ccggacgggg cccgctggcc ggcccggtgg
      661 tttcagcctg cgtgatctgg gagagagcgc cggaagacaa gaacggtgtg accgactcga
      721 agcttttgac acagaaagag cgagtcggtc ttttttcatg gataatggac aacgcgctca
      781 gtgtcggtat aggcatggcg agccgggaag agatcgacga gatgaatatc ctgaaggcca
      841 gcctgctttc catggagcgt gccttgctgg caacggggat ggaacccgat gtggtgctcg
      901 tggacggcaa ccggggtctc aggaattacc ccaatgcccg gcccgttgtg cgcggggacc
      961 gcaggtgttt cttcatggct agcgcctcca ttgtcgccaa ggtcatccgt gacagggcca
     1021 tggacgcgta ccaccgtatc tatccgaact acaatttcaa gcagaacaag ggctacccga
     1081 cgcgggagca caaggccgcg atcagggaat ttgggataac acccatccat cgcgtgacct
     1141 tcaagggggt gagggagtac tgtgccggat aaactgacga cagggcggga aggagaggac
     1201 cgagcggcgg ccgttctgat aagggagggc taccgcatcc tcgacacgaa cttccgaagc
     1261 cccttcggag agatcgacat cgtcgccgaa gagagcggct gtcttgtctt tgtcgaggtc
     1321 aaaagacgca ggggccgcgg tttcggcacg tctttcgatg ccatagacgc ccgaaaaaaa
     1381 acgcacatca tacgttccgc ccaggtctat ctgaagaaga accggtgcgt cgaccggcgt
     1441 atccgtttcg acgtcgtcgg catcgacggt gacacggtcc gcgtgatacg ccatgctttc
     1501 ggagaagaga aaggatgata gcaacagagt ggtttcctcc ataccttcct ggaagctcat
     1561 ccgtcatccc ggcggagacc tcttcgactt tcttcccgat caattcatcc atactggttc
     1621 aacaggactt cctggattcc ggccttcgcc ggaatgacgg attggaactg gttgtcggct
     1681 ttcataagcc tggacctctg aggtcgacat gatcgacgag aaggtcatgg agcttctggc
     1741 ggccgtgagg gatggacgga tctccgtcga ggacgcatac gagcgcttcc gggacgtgcc
     1801 tttcgaggac ctggagcaca ccagggtgga ccatcaccgg gtgctccgca agggtgttcc
     1861 cgaggtcgtc ttcggcgagg ggaagaccct tccccagatc tacgacattg tcggctcctt
     1921 cagacagaag gacctcgatg tcctcgtgac gcgcatcgat gccgccatcg gtgaagccct
     1981 gacgaagagg ttccccgaag ggacctattc cggcgacgcc cggtgtttct gggtgcgcag
     2041 ggaacgccct gcggaaggca agggcatggt ccttgtgatg tcggcaggaa cgagtgacgc
     2101 gaaagtggcc gaggaggcct acgtggcgtc gacattcttc ggcaatgata cggaaaagct
     2161 ttacgatgtg ggggtggcgg gcatccaccg gctcttccag aacctgccca ccttaaggcg
     2221 ggcccgggtc atcattgtgg cggcgggcat ggaaggggcc ctcccgtcga tcgttgccgg
     2281 catcgtgggc gtgcccgtca tcgccgtgcc cacgagcgtg gggtacgggg cgagcttctc
     2341 gggcctcacg gcgcttctcg cgatgctcaa ctcctgctcg accgtggccg tcttcaacat
     2401 agacaacggc tttggcgcgg cctatttcgc gacactggta aaccggttat gaagatactt
     2461 tacatagatc ccgtattcgg aataagcggc gacatgatga tcggctctct catcgattcc
     2521 ggcgtcccct tcgacgccct tgaggcgctc gtcagaaaga tatcgcctga cgcgccctcg
     2581 atgaagcccg tccggctcac tgagggcgtc ataagcggca tccatctgga gatagggcag
     2641 tcggacaggt tctacacgat atccgagatg gaacaggtca tggggagcat cgatgtcgag
     2701 aagaaggtga gggacgatgc gctgggcatg ctttctctca tcgtggccgc tgaatcgaag
     2761 atacacggca tgtcgcgcga cgaactccac ctccacgagc tttcccacat agacacggtc
     2821 atagatctcc tgtgtgtcgc gtacggcgtc aggttccttg gagctgacag ggtgttctgc
     2881 gggccggtgc cctgcggcag cggcaccatc accacgtccc atggctccat tcccaacccg
     2941 ccgccggtga cgctggagat cctccggggc cacccgcttg tattttacgg tgagagcctg
     3001 gaactgacga cccccacggg ggccgccatc gtcgctcatt acacggaacc cgtcggcagg
     3061 gtgccggcct tcaggaccat ccgcgagggt tatggggtcg ggacgtacaa atcctcgcgg
     3121 cccgatgtcc tcaggatctt tgtgggagag gcggacgaac ccgcctatga ccacgaggtc
     3181 gaggtgatcg aggcggatat cgacgacatg gaaatggaat acatgggggc tgtggccgac
     3241 agactccgcg ccgtcggcgc actcgatgta ctcttcttcc ccgtctcgat g
//
LOCUS       DAOPOA010000183         3279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_31760_length_3279_cov_2.476427, whole genome shotgun sequence.
ACCESSION   DAOPOA010000183 DAOPOA010000000
VERSION     DAOPOA010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31760_length_3279_cov_2.476427"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PKJ17_04920"
     CDS             <1..946
                     /locus_tag="PKJ17_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75345.1"
                     /translation="GTISVDRSSLGGARFLMTFPALQNEPSSSQEDSDMMPSMKVVIL
                     GGSAAGPKVASKVFRLMPNADITILDRGQVLSYAGCGLPYYISGLVDDIEEIGEEIEL
                     IAVNARVKSAHTGVEGAPLGVIAEAIWHLSIDATSQKTTISELLREVVTATEGLQAAV
                     REGTGDGGSDSGSIIGELSGLLNELKGMNDGVVGLVKEIEGGSRSLSGLIEETIESIS
                     VHKDLRREVSGLSALFDEVIAAARRAVSHEELEKARGLSLEYIASNYTMQRERIIHQS
                     VASNVIPFTGRDRGDVTPPAPEEGGACDDDLGDNVELF"
     gene            999..1364
                     /locus_tag="PKJ17_04925"
     CDS             999..1364
                     /locus_tag="PKJ17_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75346.1"
                     /translation="MTKRIMTVDDSVSVRQMVSFTLKNAGYETVEASDGQDALSKLGG
                     SGIHMIVTDLNMPNLDGIGLIKEVRNKAEYKFIPIIMLTTESQDSKKQEGKSAGATGW
                     IVKPFKPDQLLAVVKKVLG"
     gene            1377..2291
                     /locus_tag="PKJ17_04930"
     CDS             1377..2291
                     /locus_tag="PKJ17_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOD75347.1"
                     /translation="MALPEDSRSRNADYGSIRLSNRLFEELTVFIRDQVGIKITPVKK
                     VMLEGRLQKRLRKLGMRSFDEYCKYLFSDEGRGGELTSMIDEVTTNKTDFFREPAHFN
                     YLTSRVLPTLLRGGGFTVSNRLTIWSAGCSSGEEPYTIAMVVQDFADTAGAERLPFHV
                     VATDISRRVLEKGQKAVYEDDKVAPIPVAIKKKFLLKSRNPEAGLFRIAPEIRSRVSF
                     RRLNFMDGDFGFREKIDIIFCRNVLIYFDKIVQERLLNKFCRCLKPDGYIFMGHSETL
                     FGMDLPLEQVAPTVYRKTGTIATIRKTA"
     gene            2311..>3279
                     /locus_tag="PKJ17_04935"
     CDS             2311..>3279
                     /locus_tag="PKJ17_04935"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOD75348.1"
                     /translation="MMAEKIKVLIVDDSAVVRQVLEEVFQSDGALQVVGSARDPFVAA
                     DIMRRVVPDVITLDVEMPRMDGITFLHKIMSQRPIPVVMCSSLTEKNSETAMKALEYG
                     AVDIIEKPRMGVKQYLEESKVIICDTVKAAAEARLGRAKKNNGFIKVEPKLTADAVIE
                     KPNSRAMIQTTERVVVVGASTGGTEALKSFLEALPLDAPGMVIVQHMPEHFTAAFAKR
                     LDGICRVSVKEARNDDTVVRGRVLIAPGNKHTLLKRSGARYYVEVKDGPLVSRHRPSV
                     DVLFRSAARYAGKNAVGVIMTGMGDDGAKGMLEMKDAGAYTVAQDEA"
BASE COUNT          749 a          892 c         1032 g          606 t
ORIGIN      
        1 cgggaccata tccgtggatc gttcttccct gggcggtgcc cggttcctca tgacctttcc
       61 ggcgctgcaa aacgagccta gttccagcca ggaagattct gacatgatgc cgtctatgaa
      121 agtggttatc ctcggcggct cagccgccgg ccccaaagtg gcctccaagg tatttcggct
      181 gatgcccaac gcagacatca ccattctgga tcgcggccag gtactctcct acgccggctg
      241 cggactgccg tactacatct ccggcctcgt ggacgacata gaagagatag gcgaggagat
      301 agagctcatc gccgtcaatg cccgcgtcaa gtccgcgcac accggggttg agggcgcacc
      361 gctcggggtc atagcggagg cgatatggca cctttccata gatgccacgt cacagaaaac
      421 gaccatatcc gagctcctca gggaggtcgt gacggccaca gagggacttc aggccgccgt
      481 gcgagagggg accggcgacg gcggctctga cagcggcagc attatcggcg aactgtcggg
      541 gctgctcaac gagctcaagg gcatgaacga cggggtcgtc ggtctggtga aggagataga
      601 ggggggcagc agaagcctgt ccggcctcat cgaggaaacg atcgagagca tctccgtcca
      661 caaagacctc agacgtgagg tgtcgggcct ctcggcgctc ttcgatgagg tcatcgccgc
      721 ggcaagacgc gccgtgtccc atgaggaact cgagaaggcc cgcggccttt ccctggagta
      781 catcgccagc aattacacga tgcagaggga gaggatcata caccagagcg tggcatcgaa
      841 tgttattccc tttacgggaa gggatagggg agacgtaacg cccccggccc cggaagaggg
      901 tggtgcctgc gacgatgatc ttggtgacaa cgtggagctc ttttgatgat gaacaccgtg
      961 aatctataca tgcattttga tccacaagga ggagtgcggt gacgaaacgt atcatgactg
     1021 tcgatgattc tgtaagcgtc cgccagatgg tcagtttcac cctcaagaac gcggggtatg
     1081 agacggttga ggcaagcgac ggacaggacg cactgtcgaa gctgggcggc tcggggatcc
     1141 atatgatcgt gaccgacctc aacatgccga accttgacgg gataggactc atcaaggaag
     1201 ttcgaaacaa ggccgagtac aagttcatac cgatcatcat gctgacgacg gaatcgcagg
     1261 acagcaagaa gcaggaaggc aagagcgccg gcgccactgg ttggatagtg aaacctttca
     1321 agccggacca gctcctggcg gtcgtgaaga aagtcctggg ataggaggta agggtgatgg
     1381 cgctccctga ggattcgcga agccggaacg ccgactacgg ctccatcagg ctgtcgaaca
     1441 ggctcttcga ggaactgacc gtcttcattc gcgaccaggt ggggatcaag atcacccccg
     1501 tcaagaaggt catgctcgag ggaagacttc agaagaggct gcgcaagctg gggatgagat
     1561 cgttcgacga gtattgcaag tacctgttca gcgacgaggg gcgaggcggc gagctcacga
     1621 gcatgatcga cgaggtgacg acgaacaaga cggatttctt ccgcgaaccc gctcacttca
     1681 actacctcac ctcgagggtc ctgcccacgc tcctcagggg cgggggattc accgtctcga
     1741 acaggctgac catctggagc gcaggctgtt cgagtggcga agagccctac accatcgcca
     1801 tggtggtgca ggacttcgcg gatacagcgg gcgcggagcg cctgcctttt cacgtggtcg
     1861 cgaccgatat ttcccggcgt gttctggaga aaggccagaa agccgtctac gaggatgaca
     1921 aggtggctcc cataccggtg gcgatcaaga agaagtttct gttaaagagc aggaaccccg
     1981 aggcggggct cttcaggata gcccccgaga tacggtcccg ggtctccttc aggaggctca
     2041 atttcatgga tggcgatttc ggcttccggg aaaagataga catcatcttc tgcaggaatg
     2101 tgctcatcta tttcgacaag atcgttcagg aaaggcttct caataagttc tgccggtgtc
     2161 tgaagcctga tgggtacatc ttcatgggcc attccgagac gctcttcggc atggaccttc
     2221 ccctcgaaca ggtagcgccg acggtgtaca ggaagaccgg cacaatagcc accattagaa
     2281 aaacagcatg aacagggcgt aagcggaggc atgatggcag agaagatcaa agtcctgatc
     2341 gttgacgatt cagcagtggt gagacaggtg cttgaagagg ttttccaatc cgacggagcg
     2401 ctgcaggtgg tcggctccgc cagggacccc tttgttgccg cggacatcat gagacgtgtc
     2461 gtccctgatg tcatcacgct cgatgtggaa atgccgcgga tggacggcat caccttcctt
     2521 cacaagatca tgtcccagag gcccatccct gttgtcatgt gctcgagcct cacggaaaag
     2581 aattccgaga cggccatgaa ggcgctggag tatggggcgg tggacatcat agagaagccc
     2641 cggatgggtg tgaaacaata cctcgaggaa tcgaaggtga tcatctgcga tacggtgaag
     2701 gcggcagccg aggcacgcct cgggcgcgcg aagaagaaca acggtttcat aaaggtggaa
     2761 ccgaagctca ccgctgatgc cgttatcgag aagcccaact cccgcgccat gatccagaca
     2821 acggagcgtg tcgtcgtcgt gggtgcgtcg acaggcggaa cggaggcgct caagtcgttt
     2881 ttggaggccc ttcctcttga cgctccggga atggtcatcg tccagcacat gccggaacac
     2941 ttcacggccg cctttgccaa gcggctcgat ggcatttgcc gggtttccgt caaggaagcc
     3001 cggaacgacg acactgtggt ccggggacgg gtcctcatcg ctccgggcaa caagcacacc
     3061 ctcctcaaga gaagcggcgc caggtactat gtcgaggtga aggacgggcc gctggtatcg
     3121 cgtcacaggc cttccgtcga tgtcctgttc cggtcggcgg cgcgctatgc cgggaagaac
     3181 gccgttggcg tcatcatgac agggatgggt gacgacggag ccaaggggat gctcgaaatg
     3241 aaggatgccg gcgcttacac ggtggcccag gacgaagcg
//
LOCUS       DAOPOA010000184         3266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_31919_length_3266_cov_3.911865, whole genome shotgun sequence.
ACCESSION   DAOPOA010000184 DAOPOA010000000
VERSION     DAOPOA010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31919_length_3266_cov_3.911865"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PKJ17_04940"
     CDS             complement(<1..920)
                     /locus_tag="PKJ17_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD75349.1"
                     /translation="MGLVRIHDVSLSFGGPRLLDCATIQIESGERIGLLGRNGSGKST
                     FMKLIAGEVHPDSGEVVYGGDVRVALLPQDVPDDLPGTVYDVVASGGFEHARLLSKYH
                     DLTMRIARGQDDGLLKTLEQVQHLIEASGAWHYHQRINAVIERTALDENAPFRTLSAG
                     TKRRVFLAKALVADPDLLLLDEPTNHLDVATILWLEEFLLRYGKTVIFVTHDRAFLKR
                     LATRIVEIDRGRLTSYTCDYPAYLERRQALLEAQEKQWSEFDKKLAREEVWIRQGVKA
                     RRTRDEGRVRALMRMREERAARQEQTGLSR"
     gene            complement(975..2090)
                     /locus_tag="PKJ17_04945"
     CDS             complement(975..2090)
                     /locus_tag="PKJ17_04945"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOD75350.1"
                     /translation="MKRSPAFIIPFAVITAILFLFLAADCPAAAPELTPGKIDEVVTK
                     IMKEKNIPGLSLAISTPGRTIERSYGIANMEYNIPVEKDTIFEIGSITKTFTAIGILI
                     LQQEGKLSVTDRITKYFPEYPQWHDITLKHLLQHTSGIREMTEIEPFKSNQAKDWNPR
                     EVIARMAEEPLDFQPGQNAAYSNIGCIILGVVIEKVTGVSYNDFLNDRILKPLGMART
                     MFGSTSAIIPKRASGYAFAGKHVNAPFASLVLPHASGGMTSTASDLVKLAKVFTAKAL
                     LTEKSVKEMFAPARLNNGTEFLLPGPVINMTFGYSLDSVRGKKVIPAKTGGISGFNSY
                     FAYFPESQAMVALTANLDNSLESLVIITDALLGLKGR"
     gene            complement(2134..2607)
                     /locus_tag="PKJ17_04950"
     CDS             complement(2134..2607)
                     /locus_tag="PKJ17_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75351.1"
                     /translation="MKKTRAIMIAAIMLLALAGAAMAVDIDTLDRVSVLMPKSEVIAI
                     LGPPDDVLDVGAGLKAEVYKVEGIEPMMGTGCIYDGDRLAGQSFMFAGEMTAGAAERL
                     KKHGFALLEEKNAAYRLLGNDDDTGRVFMVHVFSEMGMTIVMTFEKEFYDRHNKQ"
     gene            complement(2622..>3266)
                     /locus_tag="PKJ17_04955"
     CDS             complement(2622..>3266)
                     /locus_tag="PKJ17_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOD75352.1"
                     /translation="PVSTNPMGARILADGKPAGQTPGTVSLERNKDHILTIVKENYQQ
                     VDVVINRQYQSNKVLMKAVQSGVNSGLFFKNAGMGMNSGFNSISNQEATGEAYVLIPP
                     SVAVTLIPKQGQAVRPPQGSPYPAAPSAAPPARPAANTDIPTKDLVQAGVIAGAAVGA
                     SQAKPIEKKWETSSSSRTYREPDGTVVTKKSSTSVSVGVNPAGLIGALDTLFSK"
BASE COUNT          559 a          955 c         1023 g          729 t
ORIGIN      
        1 aaccgggaca ggcccgtttg ctcctgccgc gccgcccgtt cctcccgcat ccgcatgagc
       61 gcacggaccc tgccttcatc acgggtgcgg cgggccttga cgccctgtct gatccacacc
      121 tcttcccggg ccagcttctt gtcgaattcg gaccattgtt tctcctgggc ttcaagaagg
      181 gcctgcctgc gttcgagata tgcggggtaa tcgcaggtgt acgatgtcag gcgtccgcgg
      241 tcgatctcta ctatcctcgt cgccaggcgc ttgagaaacg cccggtcgtg ggtcacgaag
      301 attacggtct ttccatatct caggaggaac tcctcgagcc agagtatggt ggccacatcg
      361 aggtggttcg tcggttcgtc aaggaggaga aggtccgggt ccgcgacgag ggcttttgcc
      421 aggaacacac gccgcttcgt gcccgccgaa agtgtcctga aaggcgcgtt ctcatcgagc
      481 gcggtccgtt cgatcaccgc attgatcctc tggtggtaat gccaggcccc ggatgcttca
      541 atgaggtgct gcacctgttc gagcgttttt aagagcccgt cgtcctgccc gcgggcgatg
      601 cgcatcgtga ggtcgtggta tttgctgaga agccgggcgt gctcgaaccc gcccgaggcg
      661 acgacgtcat agacggtccc ggggaggtcg tcgggaacat cctggggaag gagcgcgacg
      721 cggacatcgc cgccgtagac gacctctccc gaatcgggat ggacctcgcc ggcgatgagc
      781 ttcatgaagg tcgacttgcc cgacccgttg cgccccagaa gacctatcct ttcaccagat
      841 tcgatctgaa tggtggcgca gtcgaggagc cgcggcccgc cgaagctcaa ggaaacatca
      901 tgtatgcgca caagtcccat gtcgctctca aggtcttcaa tatatcacga cggaagacgc
      961 aggtataccg gcccctacct gcccttcagc ccgaggaggg cgtcggtgat gatgacaagg
     1021 ctttcaagcg agttatcgag gtttgccgtc agggccacca tggcctgaga ttcggggaag
     1081 taggcgaagt aggagttgaa ccccgatatc ccgcctgtct tggcggggat caccttcttg
     1141 cccctcaccg agtcaaggct gtagccgaag gtcatgttga tgacgggtcc gggaagcagg
     1201 aactccgtcc cgttgttaag gcgggcgggg gcgaacatct ccttcacgga cttttcggtg
     1261 agaagggcct ttgccgtgaa gacctttgcg agcttgacga ggtctgaggc ggtggaggtc
     1321 atgccgccgc tcgcatgggg caggacgagg ctcgcaaagg gggcgttcac gtgcttgccg
     1381 gcgaaggcgt agcccgacgc ccgtttcggg atgatggcgc tcgtgctgcc gaacatcgtg
     1441 cgggccattc ccaggggctt caagatcctg tcgttcagga agtcgttgta ggaaacaccc
     1501 gtcaccttct cgatgaccac ccccaggatg atgcacccta tgttgctgta agccgcgttc
     1561 tgtccgggct ggaaatcgag gggctcttca gccatgcggg cgatgacctc ccgggggttc
     1621 cagtccttcg cctggttgct cttgaagggc tcgatctcgg tcatttcccg tatgcccgag
     1681 gtatgctgga ggaggtgttt gagggtgatg tcgtgccact gggggtactc ggggaagtac
     1741 ttcgttatcc tgtcggtgac gctgagcttt ccctcctgct ggaggatcag tatccctatg
     1801 gccgtgaagg tcttggtgat ggagcctatc tcgaagatgg tgtccttctc cacggggatg
     1861 ttgtattcca tgttggcgat gccgtagctt ctctcgatcg tccttccggg cgtggatatg
     1921 gcaagggaca gccccggtat gttcttctcc ttcatgatct tcgtgacgac ctcgtcgatc
     1981 ttccccgggg tgagctcggg agccgccgcg gggcagtcgg cggcgaggaa cagaaaaagg
     2041 atggcggtga tcaccgcgaa gggtatgatg aatgccgggc ttctcttcat aatcgttcct
     2101 cctggggcct gcatctttct tccggtaagg ccgtcactgc ttgttgtgcc tgtcgtagaa
     2161 ctccttctcg aaagtcatga cgatcgtcat tcccatctcg ctgaagacat ggaccatgaa
     2221 gaccctgccc gtgtcgtcat cgttcccgag aagccgatag gcggcgttct tctcctcgag
     2281 gagggcgaag ccgtgctttt tcaatctctc ggcggccccg gcggtcatct ccccggcgaa
     2341 catgaaggac tggccggcaa gacggtcgcc gtcatagatg cagcctgttc ccatcatcgg
     2401 ctcaatgccc tccactttgt acacctcggc cttgaggccc gcgccgacgt caagcacatc
     2461 gtcgggagga ccgagtatgg caatgacctc ggatttcggc atgaggacgc tgaccctgtc
     2521 gagggtgtct atgtctacgg ccattgcagc cccggcaagc gcgaggagca tgatcgccgc
     2581 gatcatgata gcacgtgtct ttttcatcgt tccatctcct tttatttcga gaacagcgtg
     2641 tcgagggcgc ctatgagccc tgcggggttg acgccgacgc tgacggatgt gctggatttc
     2701 ttcgtgacca ccgttccatc aggctccctg taggtccttg atgacgacga ggtctcccac
     2761 ttcttctcga tgggtttagc ctggctcgcc ccgacagcag cgcccgcgat aacgccggcc
     2821 tggacgaggt ccttcgtggg tatgtcggtg ttcgccgcgg gccgtgcggg aggggcggct
     2881 gaaggcgcgg cgggatacgg gctcccctga ggggggcgga ccgcctggcc ctgtttgggg
     2941 ataagggtca cggccaccga aggcgggatg aggacatagg cctcccccgt tgcctcctgg
     3001 ttggaaatgg agttgaaccc gctgttcatt cccatccccg cgttcttgaa gaaaagcccc
     3061 gagttgaccc ctgactggac ggccttcatg agcaccttgt tcgactggta ctgcctgttg
     3121 atcacaacat ccacctgctg gtaattctcc ttgacgatgg tcaggatgtg gtccttgttc
     3181 ctctcaaggg agacggtgcc gggtgtctgg cccgcgggct tcccgtctgc cagtattcgc
     3241 gctcccatgg ggttcgtgga aacggg
//
LOCUS       DAOPOA010000185         3256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_32056_length_3256_cov_3.440175, whole genome shotgun sequence.
ACCESSION   DAOPOA010000185 DAOPOA010000000
VERSION     DAOPOA010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32056_length_3256_cov_3.440175"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKJ17_04960"
     CDS             <1..601
                     /locus_tag="PKJ17_04960"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOD75353.1"
                     /translation="VHCVAMLFSRGELKAHVVYRQLRDLPFTGGQATLRVSVDNPVAV
                     GHLRRLLERLAWHGICQADFIVDEATGIPYLIDINPRLWGSLVQGIASGVDFPYLLYT
                     LARDGDTSRVDGFTKGVVTRWVWGDLRTFPEALKSAGDKMAFLREYFRLFNRKTAFDD
                     LSLRDPLPFLTFGLDFLAKMVGQKTIHPRSHDSLEGVWE"
     gene            602..1219
                     /locus_tag="PKJ17_04965"
     CDS             602..1219
                     /locus_tag="PKJ17_04965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOD75354.1"
                     /translation="MRPAEFLNKLIHRSPLQAASAVFRFARGYVISCLNFERRALVSV
                     RGGLRIVRRDGTISVGDMTDLWPGVKLSCQGRGGVDRARIRIGRNCSIGDRTEIHAGR
                     LIDIGHGSIIAWDCVIMDRDYHSTDNGPELVRPVTIGNGVWIGCRAIILKGVTIGEGA
                     VVAAGAVVTKDVSPFTLVAGNPARAVKRVAGWRKGRPSLTISRKT"
     gene            1231..2376
                     /locus_tag="PKJ17_04970"
     CDS             1231..2376
                     /locus_tag="PKJ17_04970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD75355.1"
                     /translation="MTGPITVLHLIAPAHFGGAERVVLSLADAIDRSRFNVLIGSFVN
                     ARFPDNEFLGQVREKGISGTVFWLRRTLDIENIPRLVRFIRSEGVAIVHTHGYRSDIV
                     GLIAARLSSRPVIATIHGFVPIDSRLRVYEQCDRFALRFFDLVLPVSDQIERGLCASG
                     VRRERMTTIRNAIAPGGGDGPKRSASPLPLLKRDGDFLIGVVGRLSPEKNIPGFLEAA
                     RDLSARYGHLRFVVVGEGPEREALKGLTTRLGLDGKVRFTGFVEEMEEVYSLLDMLVI
                     SSSTEGVPLTVLEAMRHGVPVVSTGVGGIPEVIEDGVDGLIVESGDTGALCRAVETLV
                     VDGNRYMAISRNAQKKVLRDFDRSSWARRIERCYLSMLNKREESSNG"
     gene            2369..>3256
                     /locus_tag="PKJ17_04975"
     CDS             2369..>3256
                     /locus_tag="PKJ17_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOD75356.1"
                     /translation="MDKLRVGIVGCGTIFHHHQAFIRSYPGATLCAVADRDEKALKKA
                     SDVYGIANCYSDLEEMARAEATDVIHITTPPQTHAALAEKAMNLKNHVFVEKPLTLDH
                     RSAVRLYDIAAHNGVKLCVDHNHLFDPWMLKAKEVLKDLRPGDVTYVESYYGINPRIP
                     EIMGYREAGEISWIFSLPGGLFHDFLSHPLYLMLEYTGRPVKMQTMAGSCGALIQDLS
                     DELHIMVEGEKAMGKLTISFNARPFQHFLKIYHKKAIVTVDFNNMTMVANRLTGLPGA
                     VTKIAGNLGAARALTTQTFS"
BASE COUNT          644 a          944 c         1033 g          635 t
ORIGIN      
        1 ggtgcattgc gtggcgatgc tcttcagccg cggagagttg aaagcccatg tagtctacag
       61 gcagttgagg gacctcccct tcaccggcgg acaggcgacg ctgagggtca gcgtcgataa
      121 ccccgtcgcg gtgggccatt tgaggagatt actggagagg ctcgcctggc acggtatatg
      181 ccaggcggat ttcatcgtcg atgaggcaac gggcataccc tacctcatcg acatcaaccc
      241 gcgtctctgg ggctccctgg ttcagggtat cgcgagcggc gtggacttcc catacctgct
      301 ctacaccctg gcccgcgacg gcgacacgag ccgtgtcgat ggtttcacca agggtgtcgt
      361 gacgcgatgg gtgtggggag acctcaggac ctttcccgag gccttaaaga gcgccggaga
      421 caagatggca tttctgagag agtacttccg gctcttcaac aggaagaccg ccttcgacga
      481 tctcagtctc agggaccccc tccccttctt gaccttcggc ctcgatttcc tggcaaagat
      541 ggtgggacag aaaacgatcc atccccggtc ccacgattcg ctggaaggtg tctgggaata
      601 gatgagaccc gcagagttcc tgaacaaact gatacacagg tccccccttc aggccgcgtc
      661 ggctgttttt cgcttcgcgc gggggtatgt catctcgtgc ctgaacttcg agagacgcgc
      721 gctggtgtcg gtgcggggcg ggctccgcat agtgaggcgc gacgggacca tatcggtggg
      781 cgacatgacc gacctctggc cgggggtgaa actctcctgc caggggcgcg gcggtgttga
      841 cagggcccgc atcaggatag gcaggaactg ttccatcggc gacaggaccg agatacatgc
      901 cggcaggctc atcgatatcg gccatggctc gatcatagcc tgggactgtg tcatcatgga
      961 ccgggactac cattcgaccg acaatggtcc cgagctggtc aggcccgtga cgatcgggaa
     1021 cggggtctgg atcggttgcc gggccatcat cctcaaaggg gtgaccatag gcgagggggc
     1081 ggtcgtggca gcgggcgccg tggtgacaaa ggatgtctct cccttcaccc tggtcgcggg
     1141 caacccggcg cgggccgtaa aaagggtggc gggttggcgc aagggccggc cctccctcac
     1201 tatctcacgg aagacatgac aggagaaccg ttgacaggac cgatcaccgt cttgcacctt
     1261 atagccccgg cgcatttcgg aggggcagaa agggtcgtcc tcagccttgc ggacgccata
     1321 gaccgttcgc ggttcaatgt tctcatcggt tccttcgtga atgcccgttt tcccgacaat
     1381 gagttcctgg ggcaggtgcg tgagaagggg atctccggaa ccgtcttctg gttgaggcga
     1441 accctggaca tcgagaacat tccccgtctc gtgaggttca tccgttccga aggggttgcc
     1501 atcgttcata cccacgggta ccgctccgat attgtcggac ttatagccgc caggctgtcg
     1561 tcccgccccg tgatcgcgac gatccacggc ttcgtgccca tcgactcgcg gttgcgcgtc
     1621 tacgagcaat gtgatcgctt cgcgctgcgc ttcttcgatc ttgtgctacc cgtctcggac
     1681 cagatagagc gggggttgtg cgcaagcggt gtgcgcaggg agaggatgac cacgatccgt
     1741 aacgccatcg cacccggggg cggtgacggg ccgaagcgtt cggcctcccc gcttcccctt
     1801 ctcaagcgtg acggagactt tctgataggc gttgtcggaa ggctgagccc cgagaagaac
     1861 ataccggggt ttctcgaagc ggccagggac ctgtcggcaa ggtacggtca tctcaggttc
     1921 gtcgttgtcg gcgaaggacc ggaacgggag gccctcaagg gcctgacgac gaggttgggt
     1981 ctcgacggaa aggtgagatt caccgggttc gtcgaggaga tggaagaggt ctattcgttg
     2041 ctggacatgc tcgtcatctc ctcgtcaacg gaaggggttc cgctcacagt cctcgaggcg
     2101 atgaggcacg gtgtaccggt ggtttccacc ggggtgggcg gaataccgga ggtcatcgag
     2161 gacggcgtgg acggcctgat cgtggagtcg ggagacacgg gggcgctctg ccgggccgta
     2221 gaaacgctcg tcgtcgacgg caacaggtac atggcgatat cccgcaacgc tcaaaagaag
     2281 gtccttcgcg attttgaccg ttcttcatgg gcgcggagga tcgaaagatg ttatctgtct
     2341 atgctcaata aaagggagga atcgtcgaat ggataagctc agagtcggta ttgtcggttg
     2401 tggaacgatc tttcaccacc accaggcctt cattcgctcc tatcccggag ccaccctgtg
     2461 cgcggtggcg gacagggacg agaaggcgct gaagaaggcg agcgacgtgt atggcatcgc
     2521 gaactgctac agtgacctcg aggagatggc ccgcgcggaa gcgacggatg tcatccacat
     2581 caccacgccc cctcagacac acgccgcgct ggccgagaag gccatgaacc tgaagaacca
     2641 cgtcttcgtc gagaagccgc tgacactcga ccacaggagc gccgtgaggc tttacgacat
     2701 cgcggcgcac aacggtgtga agctgtgcgt cgaccacaac catctcttcg atccctggat
     2761 gctgaaggcg aaggaggtat tgaaagacct ccgtcccggg gacgtcacct acgtggaaag
     2821 ctactacggc ataaaccccc gcatccccga gatcatgggt taccgggagg cgggcgagat
     2881 atcctggatc ttttccctgc cgggaggcct tttccatgat ttcctttccc atcccctcta
     2941 cctcatgctg gagtacacgg gcaggcccgt gaagatgcag acgatggccg gctcgtgcgg
     3001 ggcccttatc caggacctgt ccgacgagct ccacatcatg gtggagggtg aaaaggccat
     3061 gggcaagctg accatctcct tcaacgcgag gcctttccag catttcctga agatctacca
     3121 caagaaggcc attgtcaccg tcgacttcaa caatatgacc atggtagcca acaggctgac
     3181 ggggttgccg ggggcggtta cgaagatagc aggcaatctc ggcgcggccc gcgcgctgac
     3241 cacgcagacg ttctcc
//
LOCUS       DAOPOA010000186         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_32088_length_3254_cov_2.606127, whole genome shotgun sequence.
ACCESSION   DAOPOA010000186 DAOPOA010000000
VERSION     DAOPOA010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32088_length_3254_cov_2.606127"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..>3254)
                     /locus_tag="PKJ17_04980"
     CDS             complement(324..>3254)
                     /locus_tag="PKJ17_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOD75357.1"
                     /translation="LPGQGIVARKMGDVLIQTGDNGQAARVLERALGILKGGADPVEE
                     GNVYRSLGNLAMRQRDHKTALGFFELALARYTTAGDKKGEADIYRGFGETALRTGDSE
                     TAGEEYSKALALYDTTGCHVGQGYARKGLADIAFFSGKNEEARRLYDMALESFTAAGY
                     PLGRADVLRRMGQLGLRRGNIPEARASYEKALPIYRKVREPVGQADVYKGLGDIGYYG
                     RNFSSALEMYDKALPYYVHAGEPLGQGNVHRATGDVYFYAGDYTKAMEYYEKALSFYM
                     KANSPMGQANTYRTMGETYLRVGKADHALAMFTSAMALYKKVNEPIGQGDIYKTLGEI
                     YLERGNRMGALDMFREALNYVTAAHSVVDQGHAYQGIGDVFLSAGDLGRSLENYDTAL
                     ELYRRMQDKESEAFVLIKKAGLAGRKGDVKEATGLFEAGLGKLEEVRSQAMFPEMKKS
                     YMEKVYGHYEDAAIFMLEANENEKAFRSIEAMKARVFLDQLAEARVDLEKGIDPAVKT
                     ERSTLTNELTIAGRKLGEESRKDAPDKEVVEKLKREFSLIQERLDALKRDIRYKNPLY
                     ASVQYPEPVAVKFLQEKVLKEGEVLAEYFLSKKGVYCLVVSRKDFNVVKLSVNHEDLL
                     SKVKAFLTNIRGYQQKERFRDALAEDLYAILVKPLETFLDGKTLIVAPQGILAYLPFE
                     ALMTEEDGKKVFMIEKYPVTYVQSGTVLSVLRSQYEREGSAGGFVGFGDPVYDYDEYL
                     SETPEARDKKAEKKNGAVSHSAGDEPPAVSPGAAFTRNSYLRAGGTLTRLQGSGEEIE
                     GIRKIYEEQGGPARSFLRIEAREENARSPEMGRYAFIHFSTHGILEPGFQAIALSQIP
                     GDREDGFLTLGEIMNSRFNARLVVLSACETGLGELSYSEGVTGLTRAVMYAGSAAAVV
                     SLWSVADEGTRDLMIRFYDGLVKKSISKVEALRAAKIELLKDTKRNAFTHPFFWSAFV
                     MYGE"
BASE COUNT          592 a          992 c          924 g          746 t
ORIGIN      
        1 aaggagatca ccgaggtggc gaagacgaaa agcaagggct gggtggacta caagtggata
       61 aaccccgtga cgggcaagat cctggaaaag accacctact gccagcgagt aggcgacgtc
      121 tacatcgggt gcggaatcta taagtaaaga cggcttgagt cgcttgaata gcgtgagtgt
      181 tagaagcctc ttttaacgag agccttgtct ttggccctta acaacaggtt atcggagctg
      241 aagagcctcg tccggaaaag ctattggctt taactctcaa gcgattcaag ccattcaagc
      301 cattcaagcc attcaagccg ttttcactct ccatacatca cgaacgccga ccagaagaag
      361 gggtgggtga aggcgttcct tttcgtgtcc ttgaggagct cgatcttcgc ggcccttagg
      421 gcttcgacct ttgatatgct cttttttacc agaccgtcat agaagcggat catcaggtct
      481 ctcgtgcctt cgtcggcgac gctccagagg cttacgaccg ccgcggcgct gccggcgtac
      541 atcacggctc tcgtgagccc cgtgacgccc tcggaatagc tcaactcgcc aaggccggtc
      601 tcacaggccg acaggaccac gagacgggcg ttgaaacggc tgttcatgat ctctcccaaa
      661 gtcaggaacc cgtcttccct gtcccccggt atctggctca acgctatggc ctggaacccg
      721 ggttcgagga tgccgtgggt ggagaagtga atgaaggcat accggcccat ctcgggagac
      781 ctggcgttct cctcccgggc ctcgatcctc aggaatgaac gggcagggcc gccctgttct
      841 tcgtatatct tccggatccc ttctatctcc tccccgctcc cctgaagccg tgtcagcgtg
      901 cctccggcac ggagatagct gttcctcgtg aatgccgcgc ccgggctcac ggcggggggt
      961 tcatctccgg cgctgtggct cacggcaccg ttctttttct ccgctttctt gtcccgcgct
     1021 tcaggggtct ccgacaggta ctcatcgtag tcgtaaacgg ggtctccgaa accgacgaag
     1081 ccgcccgctg atccctcgcg ttcgtactgg ctgcgcaata ccgagaggac cgttcccgac
     1141 tggacataag tgacgggata cttctcgatc atgaatacct tcttcccatc ttcctcagtc
     1201 ataagggcct caaaggggag ataagcaagg atcccctggg gcgccacgat gagcgtcttg
     1261 ccgtcgagga aggtctcgag aggtttgacg aggatggcat acaggtcctc tgcaagggca
     1321 tcgcggaaac gctctttctg ctgataaccc ctgatattgg tgaggaacgc ctttactttg
     1381 gacaggaggt cttcgtggtt cacggagagc ttcaccacgt tgaaatcctt cctgctcacg
     1441 acaaggcagt agaccccctt cttcgagagg aagtactcgg cgaggacttc gccttcctta
     1501 aggacctttt cctggaggaa ttttaccgcg actggctccg ggtactggac cgaggcatac
     1561 agggggttct tgtaccggat gtccctcttc agggcatcga gtctttcctg gatgagagag
     1621 aactccctct tcagtttctc cacgacctcc ttgtcgggtg cgtcctttcg ggattcctcc
     1681 ccgagtttcc tgcccgcgat cgtcagttcg ttcgtgaggg tgctgcgctc cgtctttacg
     1741 gccgggtcga tgcctttctc gaggtcgact cgcgcctccg cgagctggtc gaggaatacg
     1801 cgggccttca tcgcttcgat ggagcggaag gccttctcgt tctcgttggc ctcaagcatg
     1861 aagatggccg cgtcctcgta atggccatag accttttcca tgtaactctt cttcatttcg
     1921 gggaacatgg cctgagagcg aacctcttcg agcttcccga ggccggcctc gaaaaggccc
     1981 gtcgcctcct tcacgtcccc cttgcggcct gcgagaccgg ccttcttgat aaggacaaag
     2041 gcttccgatt ccttgtcctg catccggcgg taaagctcaa gggccgtgtc atagttctcg
     2101 agagacctcc cgaggtcgcc cgccgacaga aagacgtcgc cgatgccctg gtacgcgtgt
     2161 ccctggtcca cgacggaatg ggcggccgtg acgtagttca gggcctcccg gaacatgtcg
     2221 agagcgccca tcctgttgcc cctctcgagg tagatctcgc cgagggtctt gtatatatcg
     2281 ccctgcccga tgggttcgtt gaccttcttg tagagggcca ttgcggacgt gaacatggca
     2341 agagcgtggt cggccttgcc gactctcaga taggtctcgc ccatggtgcg gtaggtgttt
     2401 gcctggccca tcggggaatt tgccttcata tagaaggaga gggccttctc gtagtactcc
     2461 atggccttcg tataatcacc ggcgtaaaag tagacgtctc ccgtggcgcg atggacattg
     2521 ccctgcccca ggggttcgcc cgcatggaca tagtaaggca gggccttatc gtacatctcg
     2581 agggcgctgg agaagttcct gccgtaatag cctatgtcgc cgagcccctt gtacacgtct
     2641 gcctgcccca cgggttccct gaccttcctg tatatgggaa gggctttttc gtatgatgcg
     2701 cgggcctctg gtatgttgcc cctgcgcaga ccgagctggc ccatccttcg gaggacatcg
     2761 gcccgcccca aagggtatcc cgctgccgtg aagctctcaa gggccatgtc gtagagtctg
     2821 cgggcctctt cgttctttcc ggaaaagaag gcgatatcgg ccagaccctt gcgggcgtat
     2881 ccctgcccga cgtggcaccc cgtcgtatcg tagagggcaa gtgctttgga atactcctcg
     2941 ccggccgttt cactgtcccc cgtcctgagg gctgtctcgc cgaagccccg gtagatgtcg
     3001 gcctcgccct tcttgtcgcc ggcggtcgta tatcgcgcca gggccagctc aaaaaaaccc
     3061 agggccgtct tgtggtcgcg ctgcctcatt gcaaggttgc cgaggctgcg atacacgttt
     3121 ccctcctcga cagggtcagc ccccccttta aggatgccaa gggccctttc gaggacccgt
     3181 gctgcctgcc cgttgtctcc cgtctgtatc agcacatccc ccatcttgcg tgcgacgatg
     3241 ccctggccgg gcag
//
LOCUS       DAOPOA010000187         3253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_32094_length_3253_cov_3.641338, whole genome shotgun sequence.
ACCESSION   DAOPOA010000187 DAOPOA010000000
VERSION     DAOPOA010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32094_length_3253_cov_3.641338"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            475..942
                     /locus_tag="PKJ17_04985"
     CDS             475..942
                     /locus_tag="PKJ17_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75358.1"
                     /translation="MKAFEKFSRSMMTYNRIFALVLALVLMVPPAFAGSSECSVNTAP
                     GEEAPTGDAGICAFYSIQQGETNHHSANVPPASSTLFVNLNWFIPQQSLTLKIISPSG
                     STYAYYHDTDLGGGKDAQIRTRISSPEAGMWRFQVYGEEVQDTQSYFFEAATE"
     gene            983..1729
                     /locus_tag="PKJ17_04990"
     CDS             983..1729
                     /locus_tag="PKJ17_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOD75359.1"
                     /translation="MVNQPALARFALFLIVFTLLITPGSATSTILSEGEFPEMVPDDP
                     TLEYVWNIPLKLILLDFVFMTAPLLFLPVQLLISVSVWLVLGQRRISRKNALEHDTRR
                     AAYLCIRENPGINHATLSRRLGVSVGTLRYHIEILCETGQIVSENDHGFLRYYTNSRA
                     AREGKRGAYPLNETRKRMLDLLGRNPGMMRKEVASALGISGASVTWHMGLLIQSGAVR
                     SEKDGRMVRYFPGHDVVRHSRADRSVDATG"
     gene            complement(1823..2509)
                     /locus_tag="PKJ17_04995"
     CDS             complement(1823..2509)
                     /locus_tag="PKJ17_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75360.1"
                     /translation="MRRPILAVIVGVITVYACMHFFILPEAPDVTRIENITHLESPAY
                     HPAILDLWEIAVESTGVENESARLLRLETDIAGDGAIRVIWLFFYGDRDGEQHGYEAY
                     VGPGGTVSAKSQKFNFSVQGVHPLELLREVDAIALEDIPFRDRGMRLLVSVNAYGDHY
                     NETRGRLYEVSKGAFLPVKEATFGPDASWYTITITPHRESPPSGEELPDCVIAFTRQD
                     LAAADSVIYG"
     gene            2881..>3253
                     /locus_tag="PKJ17_05000"
     CDS             2881..>3253
                     /locus_tag="PKJ17_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOD75361.1"
                     /translation="MRAERVFIVAKKEFADQITGWRFLVILALFLAIALVGTYSGVGS
                     YERELDWYSQQLATMDDRFDEPGRMMMPSKPPVEGVYSSMFRTLVSYGGLLALALGFD
                     LVSKEKESRSLKSLLSHPVYRD"
BASE COUNT          693 a          950 c          953 g          657 t
ORIGIN      
        1 taccttcaac cagaggaaga gcatacgttc ccattgcctc cgggcatgcg ggacgttcac
       61 ccatccgatt cgctcttggt cgggtggacc cccgcttcgg gataccggtt ccggtatccc
      121 acgattgaga gctcaacgtt cctctataaa cgatttctgt cgaacctctc tacacatcga
      181 ggcgccctga acagtttttg ctcccagaag cgtgccgcgg ttgaacaggg atctatgcta
      241 cgccggaaat aggaaacccg cgcggcagaa atgggattca taaccccgga tcgcacacgc
      301 cctcggaatt ccgacaattg aaacccggta tttgcaggaa acgcggcaca agcgccagca
      361 ttcgtgcaag gggttcctct gccatcaccc gccccgaccc caagacggcg tgtaccgggc
      421 agccgatctg tttcagaccc gagaacgtgt agagaggatc gacagaaaac ttaagtgaag
      481 gcatttgaga agttctcccg tagtatgatg acatacaacc gcatcttcgc actggtgctt
      541 gcgctggtcc tgatggtgcc gccggctttt gccgggagca gcgaatgtag cgtgaacacc
      601 gccccgggag aagaggcccc gacaggcgac gccggtattt gtgccttcta ttctatacag
      661 cagggggaga cgaaccatca cagcgcgaat gtgcctcccg cgagttcaac gctgtttgtg
      721 aacctgaact ggttcattcc ccagcagtcg ttgacgctga agatcatctc gccgagcgga
      781 agtacctacg cctactatca cgacaccgat ctgggtggtg gtaaagacgc acagatccgc
      841 acacggatct cctctccgga ggccgggatg tggcggttcc aggtttatgg ggaagaagtc
      901 caggatacgc agagttattt ctttgaagcc gcgacggaat aaatcaaatc ttttttatat
      961 ctacggccgt ctaattctct tcatggtgaa ccaacccgcc ctcgcgaggt tcgcactgtt
     1021 cctcatcgtc ttcacgttgc tcatcacccc tgggagcgcc accagcacca tactgagcga
     1081 gggtgagttt ccagagatgg taccggacga tccgaccctg gagtacgtct ggaatatacc
     1141 gttgaaactg atcctgctcg acttcgtctt catgaccgca cccctgctct tcctcccggt
     1201 gcaactcctc atatcggtgt ccgtatggct cgttctcggt cagaggagga tctccagaaa
     1261 gaatgccctc gaacacgaca ctcgccgtgc ggcgtacctc tgcatccggg agaaccccgg
     1321 aatcaaccat gctacgctct cccgcaggtt gggggttagc gttggaacgc tccggtacca
     1381 cattgagatc ctctgcgaga cggggcagat agtatcggag aacgaccacg ggtttttgcg
     1441 gtactataca aacagcaggg cggcccgcga aggcaagaga ggcgcctatc ccctcaacga
     1501 gacgagaaaa agaatgctcg atcttctcgg ccggaatccg gggatgatgc gaaaagaagt
     1561 cgcatccgcg ctcggtatca gcggtgcatc ggtgacctgg cacatggggt tgctcatcca
     1621 gagtggtgcc gtccggagcg agaaggacgg aaggatggtg cgatacttcc ccgggcatga
     1681 tgttgtgcgg cattccaggg cggacaggag cgtggacgca accggctaga ccctcgtcac
     1741 ccaggggtgg gctgaacaac ccgggggcgc ccgggctgga gacggtgaac gagtcccggc
     1801 tctgtcggtt tcgcacgccc atctatccat agatcacaga gtcggccgcc gcgaggtctt
     1861 gccgggtgaa cgcaataaca cagtccggaa gttcttcccc ggacggcggg gactcccggt
     1921 ggggagtgat cgtgatggtg taccaggatg cgtccggacc aaacgtcgcc tctttcaccg
     1981 gcaggaacgc ccccttcgag acctcgtaga gcctcccccg ggtctcgttg tagtggtccc
     2041 cgtacgcgtt cacgctcaca agaagcctca tacccctgtc ccgaaaggga atatcctcca
     2101 gggcgatagc gtcgacctcc cgcaagagtt cgagggggtg caccccctgc acggaaaaat
     2161 tgaatttctg cgatttagcc gataccgttc cgcccggccc cacatacgcc tcgtagccgt
     2221 gctgctcgcc gtccctatcg ccgtagaaga agagccatat tacccggatc gcgccgtccc
     2281 cggcgatgtc ggtctccagt cgcagcagtc tcgccgactc gttctcgacg ccggtgctct
     2341 cgacggcgat ctcccagagg tccaggatgg ccggatggta tgccggggac tcaagatggg
     2401 tgatattctc gatacgggtg acgtccgggg cctccggtaa gatgaagaag tgcatgcagg
     2461 cgtagacagt tattactccg acgataacgg caaggatcgg tcgtctcatg gtagttccgg
     2521 gccgatggaa ccggcgtggt taaagtcagt atgggaccgg ccgggcttga agtttccgaa
     2581 aagtgaccgg tgcagagtat cgtcagttta ccacggccac tggtcggatc cactcctctc
     2641 acgccccgac gtgtagaggg atgtgagaga aaaggtatat ggagtaacga tccagtctca
     2701 agacggcaac acccgttgaa gtgcatctga gtaacccttg cccgttccgg gcggcggaaa
     2761 aacctcggtt gtcacttgag gggcgatacc gcatattgtc gcgggcctgc ggtactcttg
     2821 cgaccacgga cggagaacga gcatgaagat agatgtatgt acactgaccg gtgataacac
     2881 atgagggcgg aacgggtctt catcgtggcc aaaaaagagt tcgccgacca gatcacgggc
     2941 tggcggttcc tggtgatcct cgctctcttc ctcgcaatcg ccctggtggg aacctacagc
     3001 ggggtcggga gctacgagag agaactggac tggtattccc agcaactggc gacgatggat
     3061 gaccggttcg atgaaccggg gcggatgatg atgccctcga aaccgccggt cgagggcgtt
     3121 tactcctcga tgttccggac gctggtctcc tacgggggcc tccttgcgct tgcgctcggg
     3181 ttcgacctgg tctcgaagga gaaggagtcc cgatcgctca aaagccttct ctcccacccg
     3241 gtctaccgcg acg
//
LOCUS       DAOPOA010000188         3238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_32288_length_3238_cov_2.743010, whole genome shotgun sequence.
ACCESSION   DAOPOA010000188 DAOPOA010000000
VERSION     DAOPOA010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32288_length_3238_cov_2.743010"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKJ17_05005"
     CDS             <1..205
                     /locus_tag="PKJ17_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD75362.1"
                     /translation="DEGVVELVDVLYHTNNIVEAEKWIAFADREGISTPRLHFLKGMV
                     LAKRGRLDEAVLAFETAKNSTPD"
     gene            394..1320
                     /locus_tag="PKJ17_05010"
     CDS             394..1320
                     /locus_tag="PKJ17_05010"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOD75363.1"
                     /translation="MGYKYDTNVVTKGDGPMVESISGQEDSAFNFGARIGYTAPFSFR
                     TPFSFSAYYSVYADRYFGKTYTRSDGSRGNLTEYNNMTNTVSAVPGYAFGRFAVSLPL
                     TYSYVSLQGVKGNSFFNELNWWNQTRYLETKAVTPTLRFLTTASSFGEVFFSYMRKKY
                     FDTELHPEPPPEEERSGERMTGGLGWTYTFKENKAFLTLRYAYARDNTIGRNWVNDEN
                     RFGADLFYPILGPLRGQVSFDATYVKYSYDNTYFERKRRDDIYAVSCALLYGIVRNAD
                     IIVQYNYYRNQSNIALYDYTREVYGLGVEYRF"
     gene            1384..2994
                     /locus_tag="PKJ17_05015"
     CDS             1384..2994
                     /locus_tag="PKJ17_05015"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOD75364.1"
                     /translation="MKKTSPAGFLFAVFLVLIAAVAVDAAPIGKITRMEGRVDVLKTG
                     LRVVKSVSLGDAVDVGDIYRAKTNSRAEITFFNRNILRIAPATRVQISKYSDDGMRSN
                     QVMKLERGKVQAVSSEEFAKKVSSFAEGNTFEVHTPNAVAGIRGSGMTVGFAQMITGL
                     FFSTGKGYFYNPSAPARVVNVSAGFVSFVVGVGGAPSQPVRGNVSYVGGTGTAPGTSG
                     PGGTGTSNGSSGSALNTEVASLTSVVNTGYTFTPPPVIPNVFVGSVECLSGTYSDPLE
                     TVNISLNNVKFYGPTATGMPQSWKADSVTGTYSSVGEGSSFKGTTLSGGGMTANLILT
                     SEVDTGSDSGVWTANIASGSAPDGVGGYTGTFTFSGTASGTWSGALVGNTHQGTIGGS
                     AKGEVPSVLTQEPVAAPNTVVGSIPSLSGSYGAGALDVTLSDVKFYGSSAAVKPQIWQ
                     ATSVSGNFTGGNLAAFVNQPVTVTNGSNSAVFSVYSADGSNWRASVTNGSAPGGVGSY
                     ASPLAFTGSAYGTNTSTSLSGGASGKVQ"
BASE COUNT          688 a          901 c         1000 g          649 t
ORIGIN      
        1 tgacgaaggg gttgttgagc ttgtcgacgt gctctaccac accaacaata tcgtcgaagc
       61 ggagaagtgg atcgccttcg cggacaggga agggatcagt acgccccgtc tgcattttct
      121 caagggcatg gtgctggcaa agagggggag gctcgatgag gccgttcttg ccttcgaaac
      181 ggcaaaaaac tcgaccccgg actgacgcag caggccgagc tccagatagc cggcatctac
      241 acgcaacagg gaagatacaa ggacgcccag gagcgcttaa gggcgaccat caccctcgat
      301 cctgcgagcg acctggccct ctatgccagg gactacgaga agatcgtcgc cgacaaggcc
      361 gaacgggaga ggccctggcg gttcagcgtc gggatggggt acaagtacga caccaacgtc
      421 gtcaccaagg gtgacggtcc tatggtggag tccatatccg ggcaggagga cagcgccttc
      481 aatttcggcg cccgcatcgg ctacacggcc cctttctcct tcagaacacc cttcagcttc
      541 tccgcgtact actccgtgta cgcggacagg tatttcggga agacctacac gcgctccgac
      601 gggagccggg gcaacctgac ggagtacaac aacatgacga acacggtgag tgccgtcccc
      661 ggttatgcct ttggtcgttt tgccgtcagc ctgccgctca cctattccta cgtcagcctc
      721 cagggtgtga aggggaacag cttcttcaac gagctcaact ggtggaacca gacccgctat
      781 ctcgagacga aggcggtgac gccgaccctt cgcttcctga ccacggcgag cagtttcggc
      841 gaggtcttct tcagctacat gcgcaagaaa tatttcgata cggagcttca tcccgagccc
      901 cctcccgagg aggagcgcag cggcgaaagg atgacgggcg gtctgggatg gacgtacacc
      961 ttcaaggaga acaaggcatt tctcacgctg cggtacgcct atgcccggga caacacgata
     1021 gggcgcaact gggtcaacga cgagaaccgt ttcggcgcgg atctctttta ccccatcctc
     1081 ggtcccttaa gaggtcaggt ttcgtttgat gcgacttacg tgaaatactc ttacgacaat
     1141 acttatttcg agcggaagag gagggatgat atctatgccg tgtcctgcgc gctgctctac
     1201 ggcatcgtca ggaacgctga catcatcgtc cagtacaact attacaggaa ccagtccaat
     1261 attgctcttt acgactacac aagggaggtg tatggcctgg gagtggaata caggttctag
     1321 gtatccgttg agcacggctc ctaacagagt tatccgcggt cccgggaaga acggggcgat
     1381 gtcatgaaga aaacatctcc ggcaggtttt cttttcgcgg tcttcctcgt gttgattgcc
     1441 gccgtcgcgg tcgatgcggc accgatcggc aagatcaccc ggatggaagg cagggtcgat
     1501 gtcctcaaga caggtctgag ggttgtgaag agcgtgtccc tcggagatgc agtggacgtt
     1561 ggcgacatat accgggcgaa gacgaattca agggccgaga tcaccttctt caacaggaac
     1621 atcctcagga tagctcccgc gacccgtgtg cagatcagca agtattccga cgatggcatg
     1681 aggagcaacc aggtaatgaa gctcgagcgg ggtaaggtcc aggcggtatc gagcgaggag
     1741 ttcgccaaga aggtctcatc attcgccgag ggcaacactt tcgaggttca cacccccaat
     1801 gctgtcgccg gcatccgcgg ttccggaatg accgtgggat ttgcccagat gataacgggt
     1861 ctcttctttt ccaccgggaa aggctacttc tataacccga gtgctccggc aagggtcgtc
     1921 aatgtttccg ctgggtttgt ctcctttgtt gtgggggtgg gaggcgctcc ctctcagccc
     1981 gtacggggca atgtttccta tgtcggcgga acgggcacgg cgccgggcac gtcaggtccg
     2041 ggcggaacgg gcacctcgaa cggctcgtcg ggaagcgccc tcaatacgga ggtggccagc
     2101 ctgacgtcgg tggtcaacac gggttacaca ttcacaccac cccccgtcat ccccaacgtt
     2161 tttgtgggaa gtgtggagtg cttgagcggg acatacagcg accccctgga aaccgtcaac
     2221 atatcgctca acaacgtgaa gttctatggt cccaccgcga cggggatgcc gcagtcctgg
     2281 aaggccgatt ccgttacggg tacttattcg tcggtgggag aaggttcgag cttcaaggga
     2341 acgaccctgt ccgggggtgg gatgacagcc aatctcatcc ttaccagtga agtggatacc
     2401 gggagtgact cgggagtctg gacggcgaat atcgcaagcg gcagcgcgcc cgacggcgtc
     2461 ggagggtata ccggcacatt caccttcagc gggacggcgt cagggacatg gagtggggca
     2521 ctcgtgggga acacccacca ggggacgata ggcggctccg ccaaaggtga agttcccagc
     2581 gtgctcaccc aggaaccggt tgcggccccg aacacagtcg tggggtccat accgtcgctt
     2641 tccggcagct acggggcggg ggcactggac gtcacgctct ccgatgtgaa attctatggc
     2701 agcagcgcgg ccgtaaaacc ccagatatgg caggccacgt cggtgtcggg caactttacc
     2761 gggggtaatt tggcggcgtt cgtcaaccaa ccggtcactg ttaccaacgg gtccaactcc
     2821 gccgtttttt ccgtttacag cgcggacggt tcaaactggc gtgcatccgt caccaacggc
     2881 agcgcgccgg gcggagtggg aagttacgcg agccccctgg cattcacggg gagtgcctat
     2941 gggaccaaca cgagcaccag cttgagcggt ggtgcctcag ggaaggtgca gtaggccgaa
     3001 gtgcctgccg ggggatcggg atacgcggga tgctggaggt gtcgttgtcg ggtccccgaa
     3061 agaagcaccg attgttcatg atccggagtc acgattcctc tccgagcagc ttttgaccaa
     3121 agggagacga cattatgatg aagagatcgg ttttgggata ttgttcggta gtgctggttt
     3181 tttttcttgc cctgcctttt gttgtgaatg cggcccccat tggtacggtg acccggat
//
LOCUS       DAOPOA010000189         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_32413_length_3227_cov_3.405422, whole genome shotgun sequence.
ACCESSION   DAOPOA010000189 DAOPOA010000000
VERSION     DAOPOA010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32413_length_3227_cov_3.405422"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKJ17_05020"
     CDS             complement(<1..196)
                     /locus_tag="PKJ17_05020"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD75365.1"
                     /translation="MARSGEMSIGERIKVLRQAKNLTQEELATRAGLTKGFISQVERN
                     LTSLSVESLIGILDALDEKPS"
     gene            315..1910
                     /locus_tag="PKJ17_05025"
     CDS             315..1910
                     /locus_tag="PKJ17_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOD75366.1"
                     /translation="MQGKQLIRTFFHEADVSHVFQLPGLHTLSLNAELFSQQSIKTIT
                     PRHEANLAFMADGYARASGKPAVIVVTPGPGLGNIVSGCMEAYSDDVPLLIVHIDTDR
                     KDMGKGILHELQSPEDIFRHFIKASFRADEPSILSSHLHDALKICNTPRKGPVLVSIP
                     FSFLEREISEQPRVVQRTPAGPSASDRVQPFLADLKHILGDKRRPVLLGGKALMEPRI
                     GTLVDRICGRGLPFLTTTGGKGVTSDRNGFSFGNVMARGVVRDILASADVVIAAGTRL
                     RDVDAKRRGVRIENLVHIDVDDRWMGKNYRTALHCAGNVEEALEGIARIVQKREFDWD
                     IAVLKRQLDLERKGMRKEPGYVLVDLVRNTVPGDTVIVCDLNMPSYWAEYYLPVYAQR
                     SFLMPRGISPIFYSLAAALGAKTARPDLPCLAVCGDGGGLPQMAELATVVKYGIPVTI
                     LVHNNNSFSILENAMRNRHDIDGSMGLVNPDFVKLARSFGIRAKRTGTLGGLKGIFLR
                     DVRWDEPFLIEFTGSVPFPPWNG"
     gene            complement(1957..2739)
                     /locus_tag="PKJ17_05030"
     CDS             complement(1957..2739)
                     /locus_tag="PKJ17_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOD75367.1"
                     /translation="MKNGRSEEKGGALFSAEEMERYSRQMMIPSIGQEGQERLRKAKV
                     CIAGAGGLGSVSAYYLAAAGVGRIRIVDRDRVEMSNLNRQILHSTGDLGEPKVLSALD
                     KLERLNPHCRVEAIRADIDEGNVFELIGGCQVVVDALDNIKTRRALNLASVRSRIPLI
                     YGGVEAFSGMASTFISGETPCFQCLFSHISDEEKTRGVVGPLPAIIGSIQALEVMRIL
                     LGERPSLAGTLLYFSGTNMTFQNITVERNPLCPVCGDPVRQE"
     gene            complement(2750..>3227)
                     /locus_tag="PKJ17_05035"
     CDS             complement(2750..>3227)
                     /locus_tag="PKJ17_05035"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOD75368.1"
                     /translation="AKRNHINQFHRAVSSYLYEELEERHIEECLALQERWCLLRRCEE
                     DLNLQGEWEATKEILMHHRSLGVSGAVIVIDSGVKGFTLGESLGGDMAVIHIEKADPE
                     IPGLYQFINQQFCARRWSGVKFVNREQDLGIRGLRDAKLSYGPDHFVKKYRIMLKG"
BASE COUNT          687 a          986 c          852 g          702 t
ORIGIN      
        1 tggaaggctt ttcgtcgaga gcgtcgagta tgccgataag actctcaacg gatagggacg
       61 tgaggtttcg ctcgacttgc gagataaaac ccttcgtcaa tcccgccctc gtcgccaact
      121 cctcctgggt aaggttcttc gcctgcctca acaccttgat cctttcccct atgctcattt
      181 ctcccgatct tgccataaca gtatatacgc acaaaaagtt tacagtatta aactagattc
      241 gcgttttttt tgtcaaggta atttttattt aactttcagg ttcagtatgc taaactatgc
      301 catctggaca tattatgcaa ggaaaacaac taattagaac gttttttcac gaagcggacg
      361 tttcccatgt cttccaatta cccggtttgc atacacttag cctgaacgct gagctttttt
      421 ctcaacaaag catcaaaaca ataacccctc gccatgaggc caatcttgcc ttcatggccg
      481 acggatacgc gagggcctcg ggcaaaccgg cggtcatcgt cgtcacaccc gggcccggtc
      541 tcggcaatat cgtgtcgggt tgcatggaag cctactcgga cgacgtgccc cttctcatcg
      601 tccacatcga tactgacaga aaagacatgg gcaaaggcat cctccacgaa cttcaatccc
      661 cggaggacat attcagacac ttcattaagg cctctttcag ggccgacgaa ccgtcgatcc
      721 tgtcgtctca ccttcatgat gccctgaaga tctgcaacac gccacgaaag ggaccggtcc
      781 ttgtttcgat ccccttcagt ttcctggaga gggagatctc cgaacagccc cgggtggtgc
      841 agcgcacccc ggcagggcca tcagcttccg acagggtgca gccttttctc gcggacctca
      901 agcatatcct gggagacaag cgcaggcccg ttctcctggg aggaaaggca ctcatggaac
      961 cccgcatagg gaccctcgtg gacaggatct gcgggcgcgg cctgcccttc ctgaccacaa
     1021 cggggggaaa gggcgtaacg agcgaccgca acgggttctc cttcgggaac gtcatggcaa
     1081 ggggtgtcgt ccgggacatc cttgcctcgg cggatgttgt catcgcagca ggcacacggc
     1141 tgcgtgacgt cgatgcaaag cgccggggtg tcaggatcga aaacctcgtg catatcgatg
     1201 tcgatgaccg gtggatggga aagaactacc gcaccgcact tcattgcgcg ggaaatgtcg
     1261 aagaggccct cgaagggatc gcccgaatcg ttcagaaaag agagttcgac tgggatatcg
     1321 cagtgttaaa gagacaactg gacctggaga ggaagggcat gaggaaagaa ccgggctatg
     1381 tccttgtcga ccttgtgcgc aacaccgtcc ccggggacac cgtcatcgtc tgcgatctca
     1441 acatgccgtc ctactgggcc gagtattatc tgccggtata cgctcagcgg agcttcctga
     1501 tgccccgcgg catatcgccc attttctact ccctcgcggc tgcccttggc gcgaagaccg
     1561 cgaggcccga ccttccctgt ctcgccgtgt gcggcgacgg cggaggcctc ccgcaaatgg
     1621 ccgaactggc aacggtcgtc aagtacggca tacccgtcac cattctcgtt cacaacaaca
     1681 acagcttctc catcctcgag aacgccatga gaaaccgtca tgatatcgat ggatccatgg
     1741 ggctcgtcaa ccccgatttc gtgaaactcg cccggtcctt cggcataagg gcgaagagga
     1801 caggcaccct cggcggcctc aagggcatct ttcttcggga tgtgcgctgg gatgagcctt
     1861 ttctcatcga gttcaccgga agcgttccct tcccgccctg gaacggatag ctacccgccg
     1921 tttcgcgaag gcatcgaggg cgcagtcttt cccgttctat tcctgccgca caggatctcc
     1981 gcagacggga cacaagggat tcctctcaac agtgatgttc tgaaaggtca tgttcgttcc
     2041 cgagaagtac agcaacgtgc cggcaaggga cgggcgctct cccaggagga tcctcatgac
     2101 ctccagggcc tgtatggacc ctatgatagc cggaaggggc cccaccacgc ctctcgtctt
     2161 ctcctcgtcc gaaatgtggg aaaaaagaca ttgaaaacaa ggagtttctc cggagatgaa
     2221 ggtggatgcc atccctgaaa aagcttcgac gcccccgtat atgaggggta tccggcttct
     2281 cacggatgcg agattgaggg cccttcgcgt tttgatattg tcaagggcgt cgacaacgac
     2341 ctgacaaccc ccgatcaact cgaaaacatt gccctcatcg atgtccgccc ggatggcttc
     2401 cacccggcag tgggggttca gcctctccag tttgtcgagc gcggagagga ccttcggctc
     2461 accaaggtcg cccgtggagt ggaggatctg cctgttcagg ttcgacatct cgaccctgtc
     2521 acgatcgacg atccggatcc gccccacccc ggcggcggcg aggtagtatg ccgagaccga
     2581 ccccaaccct ccggcaccgg cgatgcagac cttcgccttc ctgagacgtt cctgaccctc
     2641 ctgtccgatc gaggggatca tcatctgccg ggaataccgc tccatctcct cggcggaaaa
     2701 aagggccccg cccttttcct ctgatcttcc gttcttcatt ctccacccgt catcctttca
     2761 acattatcct gtacttcttg acgaaatggt cgggaccgta ggaaagcttc gcgtcccgga
     2821 gcccccgtat cccgaggtcc tgttcccggt tgacgaactt cacgccgctc cagcgcctcg
     2881 cgcagaactg ctgattgatg aactgataga ggccgggtat ctcaggatcg gccttttcta
     2941 tatggatcac cgccatgtca cctcccaggg actcaccgag tgtaaagccc ttgaccccgg
     3001 agtcgatgac gatgacggcc cccgacacac ccagggaacg gtggtgcata aggatctctt
     3061 tcgtcgcctc ccattcgccc tggagattga ggtcctcttc gcacctcctg aggagacacc
     3121 acctttcctg gagcgcgagg cactcctcaa tgtgtcgctc ttcaagctct tcatacaggt
     3181 atgaggagac agcgcggtgg aactggttga tgtggttccg cttggcc
//
LOCUS       DAOPOA010000190         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_32456_length_3224_cov_3.800252, whole genome shotgun sequence.
ACCESSION   DAOPOA010000190 DAOPOA010000000
VERSION     DAOPOA010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32456_length_3224_cov_3.800252"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1558)
                     /locus_tag="PKJ17_05040"
     CDS             complement(176..1558)
                     /locus_tag="PKJ17_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOD75369.1"
                     /translation="MTEKRHQPIDFTNIRDLLSVIFKRKYTILAVFVVVFGTVALYAF
                     LAPRIYEAKSILLVKLGREFMRTSEGANSSPGLSVQPETIMKSEISILTSKDLLTRVI
                     NTLGIGRIYPAIAKRSATQTDAEQAAIASFEENLRVTNIPGSGLIQVAFSHGNPSTSA
                     TVVNTLVDAFKDKHLDVFGGKTTAFLERQEKAFQERLKESENNLSAFKLKNKVFSFEE
                     QKTNLIDLLGTLDEKLKTAQNEVTELEQRAAFVRGSRWTADVPVETRNQLVALQQKEQ
                     GLLERYTENSRMVGTVRQELNAMKDAIGKATEQARSVEVARIEGQLAGARARAGSIRA
                     QMRQVEGELNALDGHGRALQGLKREAAQLEQNHQIYSRKLEESLIMDDMDRQKMVAVS
                     VVQKATVPAFPKKQKLNRSQMVGAGFFGGLAAGIALAIILELMTPGMPTPASAEKSLG
                     VPVLITVTKK"
     gene            complement(1582..2355)
                     /locus_tag="PKJ17_05045"
     CDS             complement(1582..2355)
                     /locus_tag="PKJ17_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOD75370.1"
                     /translation="MPLSALVLAAAVLLCACAPNSVINPASISQMDQQSRAYPQKEYV
                     IAAGDVLDIKFVGGAEYNETGVTVRPDGRISLALAPEMRVAGLTPGEMANQLRQKYAS
                     ELKRPEVVITVRGFSDQKVFVDGEVLLPGVVELKGPTTVMRAIAQARGLRETARLSNV
                     IIIRKDFEGKPMAANLDLGKVINGTDLSQDIYLMPYDIVYVPKSNIARLNKFVDEYIN
                     RVVPGGFPGFQQFTNPYRNSLGGFTNVYPDSGASVTVAP"
     gene            complement(2382..2633)
                     /locus_tag="PKJ17_05050"
     CDS             complement(2382..2633)
                     /locus_tag="PKJ17_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75371.1"
                     /translation="MRERCMIGTVAVLGLSLAVSGMWLAQSSRCVILEKEGSRVLVSC
                     NGKPARYIDLGGRADMYRVGDSIDDRTFPGASDRKVKEK"
     gene            complement(2835..>3224)
                     /locus_tag="PKJ17_05055"
     CDS             complement(2835..>3224)
                     /locus_tag="PKJ17_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75372.1"
                     /translation="RPGAEKRYDTIFYAKNREDLPRKLRSVLEPLLFDEHIELFRSLA
                     SLKQRLCLPAEGRVMAIIFVSDREDLMDILSMRYFLCNVEIVLILPSRKKDIVALGHE
                     LRPRFMTYKDADFGEMLSVIKKMTGGW"
BASE COUNT          575 a          987 c          903 g          759 t
ORIGIN      
        1 cccttcttct cctctatgcc gtaaatgatg gcggcaagcg cctccttgtg actcgggctg
       61 agatagagga actcggggtc cggcgttatg tggaagggct gcttccggag attgtaatag
      121 ttgagataca tgtttcacca tcccctcata ggttttttgg tcagccggcg tgctgtcatt
      181 tctttgtcac cgtgatgagg acgggcacgc ccaggctctt ctccgcgctg gcgggcgtcg
      241 gcatgccggg cgtcataagc tcgagtatga tggcaagagc tatacccgcc gcgaggccgc
      301 cgaagaagcc cgcgcctacc atctggcttc tgttgagttt ctgttttttc gggaaggcgg
      361 ggaccgtcgc tttctggaca acgctgacag ccaccatctt ctgccgatcc atgtcgtcca
      421 tgatgaggga ttcctccagt ttccgggaat agatctggtg gttctgttcc agctgggcgg
      481 cctcgcgctt gaggccctgc aatgccctgc cgtgcccgtc aagggcgttg agttcgccct
      541 cgacctgcct catctgcgcc cgtatgctgc ctgccctcgc cctcgcgccc gccagttgcc
      601 cctcgatcct ggccacttcg acactccttg cctgctcggt ggccttgccg atggcatcct
      661 tcatcgcatt gagctcctgg cgaacggtcc caaccatcct ggagttctcc gtgtacctct
      721 cgaggagccc ctgttccttc tgctgcaagg caacgagctg attgcgcgtc tccacgggaa
      781 catcggcggt ccacctggaa ccgcgcacga aggccgccct ctgttcaagc tcggtcactt
      841 cattctgggc cgttttcagc ttttcatcga gggtcccgag gagatctatc aggttcgtct
      901 tctgctcctc gaaggaaaag accttgttct tcagtttgaa ggcggacagg ttgttctccg
      961 attccttcag cctctcctga aaggccttct cctgacgctc gaggaaggcg gtggtcttgc
     1021 ccccgaagac atcgaggtgt ttgtccttga aagcgtccac gagggtgttg acgactgtcg
     1081 cggatgtgga agggtttccg tggctgaagg caacctggat gaggcccgaa ccgggaatgt
     1141 tcgtcaccct gagattctct tcgaaagagg cgatggccgc ctgctccgcg tcggtctgcg
     1201 tggcggacct cttcgcgatc gccgggtata tccgtccgat ccccagcgtg ttgatgaccc
     1261 gtgtcaagag gtccttgctt gtcagaatgc ttatctcgct cttcatgatc gtctcgggct
     1321 ggacggaaag cccggggctc gagttcgccc cctccgatgt cctcatgaac tctcttccca
     1381 gcttgacgag aagaatgctc ttcgcctcgt agatccgggg agcgagaaac gcatacaggg
     1441 caaccgttcc gaagacaacg acaaatacgg cgaggatcgt gtatttgcgt ttgaagataa
     1501 cggagagaag gtcccgtatg ttggtgaagt caatgggttg atgccgcttt tcggtcatga
     1561 ttcgtcaacc tccctttgtt gtcacggcgc cacggtcaca gaggcgcctg agtcgggata
     1621 gacattggta aaaccgccga gtgaattcct gtaggggttg gtgaattgct ggaacccggg
     1681 gaaaccgccc gggactaccc tgttgatgta ttcgtccacg aacttgttga gcctcgctat
     1741 gttcgacttg ggaacgtaga cgatgtcgta aggcatgagg tagatatcct ggctcaggtc
     1801 cgtcccgttg atgacctttc ccaggtccag gttggcggcc atgggcttgc cttcaaagtc
     1861 cttgcggata atgatcacgt tggagagccg tgccgtctcg cggagtcccc gcgcctgggc
     1921 tatcgccctc atgaccgttg tcggaccctt gagctcgacc acgccgggga gaaggacctc
     1981 gccgtcaaca aagaccttct ggtcggaaaa accacgcacc gtgatgacga cctccggcct
     2041 tttcagttcc gacgcgtact tctgtctcag ctggttcgcc atttcacccg gcgtcaggcc
     2101 tgcgactctc atctccgggg ccagggcgag ggatatccgt ccgtcgggtc tgaccgtgac
     2161 ccccgtttcg ttgtactcgg caccgcccac gaacttgatg tccagaacat ctcccgcagc
     2221 gatcacatat tccttctggg gataagcccg cgactgctgg tccatctggc tgatggatgc
     2281 gggattgatg acggaattgg gcgcgcacgc gcaaaggagc acggccgccg cgagaacaag
     2341 agctgataat ggcatccgag gtttcattat ctccacctcc ttcatttttc cttcactttc
     2401 cggtcgctcg ctcccggaaa cgtcctgtcg tcgatgctgt cgcccacgcg gtacatgtcg
     2461 gccctgccgc cgagatcgat gtatcgcgca ggtttgccgt tgcagctcac cagcaccctc
     2521 gagccctcct tttcaaggat gacgcaacgg gaagactggg cgagccacat accggagacc
     2581 gcaagggaca acccgagaac ggccactgtc cctatcatgc atcgctctct catcttcacc
     2641 tcctgggaga agttgctgcc cccttgaagg caggaaaagg atcgtttcgg ggcaggctac
     2701 cgccattcaa cctcccggct gtctcgtgca tcgtcgtccc gcgacgggga catgttcctt
     2761 tcctgcatcc attcgctgtt caagggcggt gccgcgaggg ctcatccctc acgccgcgcc
     2821 gccccgctat tccttcacca gccgccggtc atcttcttga tgaccgacag catttcgccg
     2881 aaatcggcat ccttgtacgt catgaaccgg ggcctcaact cgtgccccag tgccacgata
     2941 tccttcttcc tgctcggcag tatcagcact atttcgacat tgcacaggaa atatctcatc
     3001 gagagaatgt ccatgaggtc ctccctgtcg gaaacgaata tgatggccat caccctgcct
     3061 tcggcgggaa ggcacagccg ctgcttcagg ctcgcgaggc tcctgaaaag ctcgatgtgc
     3121 tcgtcaaaga gaaggggttc cagcacgctc ctgagcttcc tgggaaggtc ctcccgattc
     3181 ttggcgtaga agatggtgtc gtaccgtttc tctgcccccg gtcg
//
LOCUS       DAOPOA010000191         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_32574_length_3215_cov_3.418987, whole genome shotgun sequence.
ACCESSION   DAOPOA010000191 DAOPOA010000000
VERSION     DAOPOA010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32574_length_3215_cov_3.418987"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PKJ17_05060"
     CDS             complement(<1..428)
                     /locus_tag="PKJ17_05060"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOD75373.1"
                     /translation="MKRFQNWSISTKVMSISVLTIIIILAGMIFYLIPMVKGKLMEEK
                     KFALQHVTDVMVSLMASYEKRVQAGEMTRDDAQKRIISNITNVRYDGSEYFFVLNTDG
                     KLLAHGVKQELVGKNASDDLFRDPSGKYFAKDLVTVAKTK"
     gene            complement(544..2676)
                     /locus_tag="PKJ17_05065"
     CDS             complement(544..2676)
                     /locus_tag="PKJ17_05065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOD75374.1"
                     /translation="MMDNHAQTFIEEAFELLSDLEAALLELEENPSDPELIGRVFRSL
                     HTIKGSGAMFGFENVAQFTHTIETVFDDVREGKLPVTEELVSLTLKARDLIRLMIEAP
                     SDEASLPQDAQTLIDSFRRIADGGNLQQTPTQNPKTDSHGQEASSSPHHRPECTYRIR
                     FAPSADIFLTGTNPLLLIGELQFLGECNVFANLDHVPPLDEIDPERCYVSWDVILTTD
                     RGVDAIKDVFIFMDDSSMVDITIIDSEDSSLMAEEETKKIGEILVERHDIKGDELART
                     LSEKKKIGEILVDKGLVSKAKVESALIEQEHLKKVKKAPKREEEAASSIRVPAEKLDT
                     LVNLVGELVTVQARLTQMASIFDNPELVSIAEEVERLTGDLRDNTLNIRMLPIGTTFG
                     RFKRLVRDLSQELGREVEMTTEGAETELDKTVIERLNDPLVHLIRNSIDHGIEPPDVR
                     ESVGKPRAGMLHIAARHSGAYVLIEITDDGAGIDKDVIRRKAVEKGLIGADAELQDKE
                     IFSLIFAPGFSTSEKITNVSGRGVGMDVVKRTIDNLRGTVEISSEKGEGTTVTLKLPL
                     TLAIIEGLLVEIDKQFFILPLTIVQECVELSRADVERSHGRDIANIRGEIVPYIRLRE
                     SFAIEGKRPDIEQIVITEVERNRIGLVVDNVIGEHQTVIKSLGKVYRDIEGVSGATIL
                     GDGTVALIMDVAKLTDAAHAAAGFAMAS"
     gene            complement(2673..3026)
                     /locus_tag="PKJ17_05070"
     CDS             complement(2673..3026)
                     /locus_tag="PKJ17_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD75375.1"
                     /translation="MDSKVEHTDNGIMLTLYGDATIQSAVDLKGVLAREIGGGDSLTV
                     DLAYVTDCDVSLFQLLCAAHKRAVSDGKAMRVSNCSPAVSRVVSSGGFRRDIGCVAKA
                     QDGCFWQRKDGEVTR"
BASE COUNT          562 a          943 c          951 g          759 t
ORIGIN      
        1 tccttcgttt tggcaaccgt cacgagatcc ttcgcaaagt acttgccgct ggggtctctg
       61 aaaaggtcgt ccgaggcgtt cttgccgaca agctcctgct ttaccccatg ggccagcagt
      121 ttgccgtccg tgttgaggac aaagaaatac tcgctgccat catagcgcac gttcgttata
      181 ttcgagatta tcctcttctg agcgtcgtcg cgcgtcattt ctcccgcctg gacccgcttc
      241 tcgtatgaag ccataagact gaccatgacg tccgtgacat gctggagggc gaacttcttt
      301 tcttccatca gcttgccctt gaccatgggg atgagataga agatcatgcc ggctagtatg
      361 ataatgatcg tcagtaccga gatgctcatg accttggtgg atattgacca gttctgaaac
      421 cgtttcatgg aaaaacctcc ttctggatgc gggtccgtga accgcatgcc aggattaaaa
      481 tgggtatctc aataacccgg aagatcgcct gtcgcgatgc aaacacgggg tacatatgcg
      541 tgatcagctc gccatcgcga acccggccgc ggcatgggcg gcatccgtta atttggcaac
      601 gtccatgatg agggcgaccg taccgtcgcc gaggatcgtg gctcccgata ctccctcgat
      661 gtccctgtag accttgccga gggacttgat gaccgtctgg tgctccccga tgacgttgtc
      721 gacgacgagt cctatcctgt tgcgctccac ctcggttatg acgatctgct cgatgtccgg
      781 tctctttccc tcgatggcga aggactccct caggcggatg taggggacga tctctccccg
      841 tatgttcgcg atgtccctgc cgtgggacct ctccacatcg gcgcgtgaga gctcgacgca
      901 ctcctggacg atggtgaggg gaaggatgaa gaactgtttg tctatctcca cgaggaggcc
      961 ctcgatgatc gcaagcgtga gggggagctt cagggtgacc gtcgtccctt cgcccttctc
     1021 ggagcttatc tcgacggtgc ccctcaggtt gtcgatggtc ctcttgacca cgtccatccc
     1081 gacgcccctg ccggagacgt tcgttatctt ctccgaggtt gagaagccgg gggcgaagat
     1141 gagggagaat atctccttgt cctgcagctc cgcgtcggca ccgatgaggc ccttctccac
     1201 ggccttcctg cggatcacgt ccttgtcgat gcccgccccg tcgtcggtta tctcgatgag
     1261 gacgtaggcg ccggagtggc gcgccgctat gtggagcatg cccgccctgg gcttgcccac
     1321 ggactcgcgc acgtcggggg gctcgatgcc gtggtctatg gagttgcgga tgaggtggac
     1381 gagcgggtcg ttgagcctct cgatgacggt cttgtcgagc tccgtctcgg ccccttccgt
     1441 cgtcatctcc acctccctgc ccagttcctg ggagaggtcg cgcacgagcc tcttgaaccg
     1501 tccgaaggtc gtcccgatgg ggagcatcct gatgttgagg gtgttgtccc tgaggtcgcc
     1561 ggtcaggcgc tcgacctcct cggctatcga taccagctcg gggttgtcga agatcgacgc
     1621 catctgggtg agccgcgcct ggacggtcac cagctcgccg acgaggttga cgagcgtgtc
     1681 gagcttctcc gctgggacgc ggatgctcga tgccgcctcc tcctccctct tcggggcctt
     1741 cttcaccttc ttcaggtgct cctgctcgat gagggccgac tccaccttcg ccttcgacac
     1801 gaggcccttg tcgacgagga tctcgccgat cttcttcttc tccgagaggg tccgcgcgag
     1861 ctcgtcgccc tttatatcgt gtctctcgac gaggatctcg ccgatcttct tcgtctcctc
     1921 ctccgccatg agggaggagt cctcgctgtc gattatggtg atgtcgacca tgctggagtc
     1981 gtccatgaag atgaagacat cctttatggc atcgacgccc ctgtccgtcg tgaggatgac
     2041 gtcccaggag acgtagcatc tctcgggatc gatctcatcg agggggggga cgtggtcgag
     2101 gttcgcgaag acgttgcact caccgaggaa ctggagctct ccgatgagaa gcagggggtt
     2161 cgtcccggtg aggaatatgt cagccgacgg agcgaaccgt atcctgtagg tgcactccgg
     2221 tctgtggtgg ggtgacgacg aagcctcttg cccgtgtgaa tcggttttcg ggttttgggt
     2281 gggggtttgc tggaggttcc ccccgtcggc aatgcggcgg aaggaatcga tcagggtctg
     2341 tgcgtcctgc ggcaaagagg cttcgtcgga gggggcttct atcatgagac gtatgaggtc
     2401 cctggccttg agcgtgaggc ttacgagctc ttcggtcacg gggagcttcc cttcccgtac
     2461 gtcgtcgaag accgtttcta tcgtgtgggt gaactgggcc acgttctcga agccgaacat
     2521 ggcgccggac ccctttatcg tgtgcagcga ccggaacacc ctgcctatga gctccggatc
     2581 ggaggggttc tcttcaagct ccaggagtgc cgcctcgagg tcggagagca gctcgaaggc
     2641 ttcttcaatg aatgtctgcg cgtgattgtc catcatctcg tcacctcacc gtccttgcgc
     2701 tgccagaaac aaccgtcctg cgcttttgcg acgcatccga tgtcgcgcct gaagccaccc
     2761 gatgagacga cccgggagac cgcgggcgag cagttgctta cccgcatcgc ctttccgtcg
     2821 ctgaccgctc tcttgtgggc agcgcagagg agctggaaga gggaaacgtc gcagtccgtg
     2881 acgtaggcaa gatcgaccgt gaggctgtca ccgccgccga tctcccgcgc gaggacgccc
     2941 ttgagatcca ccgcgctctg gatggtcgcg tcgccgtaga gcgtcagcat aatgccattg
     3001 tccgtatgtt cgaccttgct atccattctt cgattcctcc cggcgtagtt ttactgacat
     3061 actccttttt tcgccagtta ccagtttcgg tttacctgtt tttcgctgat tcgagcgtag
     3121 gatatgtctc ataatggtgt aagagtattc ctacaaagta aagaccgttg caaccgcttg
     3181 agcgttaagg aataaagaaa gaacataaaa gtacg
//
LOCUS       DAOPOA010000192         3213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_32600_length_3213_cov_3.450602, whole genome shotgun sequence.
ACCESSION   DAOPOA010000192 DAOPOA010000000
VERSION     DAOPOA010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32600_length_3213_cov_3.450602"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PKJ17_05075"
     CDS             <1..300
                     /locus_tag="PKJ17_05075"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOD75376.1"
                     /translation="KLDVLDSFDTIKICTHYKIGKRLYEEPPMALQDFYRCEPQYEEM
                     PGWKTSIKDARRLEDLPEQTLRYIRRIEELVGVPAAIVSTGPDRESTIILRNPFA"
     gene            431..1126
                     /locus_tag="PKJ17_05080"
     CDS             431..1126
                     /locus_tag="PKJ17_05080"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HOD75377.1"
                     /translation="MATNLDITQTVSMLQYLQNGRKRADNFIVNTDATGFQDVLLRSL
                     TTGRAAEFSSAQAGPGTSSTSAVDDKETKFRKCLDFVLQKEGSRLVKEDGERGASRYG
                     ILQSTARAFGYKGSIRNITGEQVEGIYRKLWEASGSSSLPYPLCVVHFDTYVNSPAAA
                     RKILQRSQGNIGDYLAMREQRYVRLASAKPQVYGKYLKGWKNRINSLRTVVAEFERDT
                     MYARNGLTTVRKG"
     gene            1275..1484
                     /gene="rpsU"
                     /locus_tag="PKJ17_05085"
     CDS             1275..1484
                     /gene="rpsU"
                     /locus_tag="PKJ17_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOD75378.1"
                     /translation="MPAVIIREGESFESALKRFKKQCEKTGILSEIKKREHYEKPSVK
                     RKKKILAAKKRALKKLKRTMDKGLY"
     gene            1491..1937
                     /locus_tag="PKJ17_05090"
     CDS             1491..1937
                     /locus_tag="PKJ17_05090"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOD75379.1"
                     /translation="MDYKSKIEEGLKEALKKRDNIRVSVLRMLLASIKNKEVEKIRPL
                     SDEEFFGLVKTSIKQHNDSIESFRKGGRLELAEKEEKELEILKEFQPSQLSEEEILRE
                     IEEAVAAVGATGRKEMGKVIKFLMDKFPGRIDGKVLSAMVLKRLES"
     gene            1959..2468
                     /locus_tag="PKJ17_05095"
     CDS             1959..2468
                     /locus_tag="PKJ17_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75380.1"
                     /translation="MIDKTLSYLDYLKLRDIILQFSATPFSKDALGNLRPLDDIEEIA
                     RRHDRIEAVLDVIKWDGRVPFSDVPDITGVLARTAIRDSVLEPSEFLSLTNFLRACED
                     VGGFLSKVFKKSAFTDELVAGIDRLTALARRIARSVNSEGYLGQRVLRVVTHTGRSLH
                     EQGEDQETA"
     gene            2437..>3213
                     /locus_tag="PKJ17_05100"
     CDS             2437..>3213
                     /locus_tag="PKJ17_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75381.1"
                     /translation="MNRERIRKQLERIMGREAMRPIIQDDYISLRNNRYVIPLKPNFN
                     EAVQGIVHDYSHSLKTSFVEPVECVELNNSINILINEEKEEEKKVLRELTDFTRGFRE
                     ALERNVRCLAELDFFHAMALFCHEFRCVRPEVTLSGSLEVKKAVNPFIAMSRKSEAVP
                     IDIVMKESEKVMIISGPNAGGKTAALKTIGLLSLMAKTGLFIPATERPVVPLFSNVFA
                     LIGDEQDISLELSSFTAHMYAIKDLYLNAGGDELVLIDEIG"
BASE COUNT          839 a          833 c          912 g          629 t
ORIGIN      
        1 aagctcgatg tgctggactc attcgacacg atcaagatat gcactcatta caagatcggc
       61 aaacggctct acgaggagcc gcccatggcc cttcaggatt tctaccggtg cgaacctcaa
      121 tacgaggaga tgccggggtg gaagacgtcg ataaaagatg cccggaggct cgaagacctg
      181 ccggagcaaa ccctgagata cataagacgc atagaggagc ttgtcggtgt tcctgccgcc
      241 atcgtatcca cggggcccga cagggaaagc accatcatcc tcaggaatcc cttcgcctga
      301 gccggcgggg aacgctcgct gtcataaatc cgacgccatg gccggccaac ggaagcgtct
      361 gtcgaggggt ctcctgtgta acccgtcaga atctaagagg tggcatagtt gttgcaaaaa
      421 attcaccact atggctacca atctcgatat cacacagaca gtatcgatgc tgcagtacct
      481 ccagaacggt cgtaagaggg cggacaactt catcgtcaat accgatgcca cgggctttca
      541 ggacgtcctg cttcgttccc tgacgacggg ccgggctgcc gagttcagtt ccgctcaggc
      601 gggtccgggc acgtcttcca cgtcggccgt cgacgacaag gaaaccaaat tccggaagtg
      661 tctcgacttt gtccttcaga aggaaggaag tcggctcgtc aaagaggatg gggaacgggg
      721 tgcgtcccgt tacggcatcc tgcagtccac cgcgcgggcc tttgggtaca agggaagcat
      781 caggaacatc accggggagc aggtggaagg gatctacagg aagctgtggg aggcatcggg
      841 ttcatcttca ctgccctatc ctctgtgcgt cgttcacttt gacacctatg tcaacagtcc
      901 ggcggctgcc aggaagatcc tccagagatc gcagggaaat atcggagatt atcttgcaat
      961 gagagagcag cgctacgtga ggctcgcttc ggccaagccg caggtgtatg gaaagtacct
     1021 gaaggggtgg aagaacagaa taaacagcct gcgaaccgtt gtggccgaat tcgagaggga
     1081 caccatgtac gcccggaacg gcctcacgac cgtcaggaaa ggatagggcg ccgccgctgc
     1141 cggggctacc cgtagccccg cttcaaaccg tcgattgtca atgccatacc gctaaaatct
     1201 gttgactttg gctgtttttt cgggttttaa taaggagctt aaactaacgg accaatggag
     1261 ggagggaaga atggatgcca gcagttatta taagagaagg tgaatccttc gagagcgccc
     1321 tcaagagatt taagaagcag tgtgagaaaa ccgggattct ctccgagata aaaaaaagag
     1381 aacactacga aaaacccagc gtcaagagaa agaaaaaaat actcgctgcg aaaaagagag
     1441 ctctgaaaaa actcaagaga actatggata aaggtcttta ctaggagtaa atggactata
     1501 aatccaaaat tgaagagggt ttgaaagagg ctttaaagaa gagggacaac ataagggtgt
     1561 ccgtgctccg gatgctcctt gcctccataa agaacaagga agtcgagaaa ataagacctc
     1621 tgtcggacga ggagttcttt ggcctcgtca agacgtcgat caaacaacac aacgattcca
     1681 tcgagagctt caggaaaggc gggcgtctcg agctcgccga aaaggaagag aaagaactgg
     1741 agatcctcaa ggagtttcaa ccctcacagc tgtcggagga agagatcctc agggagatag
     1801 aagaggcggt cgcagccgtc ggtgcgacgg gccggaagga gatggggaag gtgataaagt
     1861 tcctcatgga caaattcccg ggaaggatag acggcaaagt gttgagtgca atggtgctta
     1921 aaagactgga gtcgtgatga caatagtcga aagcccctat gatcgataag acactatcat
     1981 atctcgatta cctcaaactc agggatatta tactccaatt ttctgcgacg cccttctcca
     2041 aggacgccct tggtaatctt cggcctcttg acgatatcga agagatcgcc aggcgccatg
     2101 accggataga agccgtcctg gatgtcataa agtgggacgg cagggtcccc ttctccgacg
     2161 tccccgatat aaccggagtg ctggcgagga ccgccatcag ggattccgtc ctcgagccct
     2221 cggaattcct gtccctcaca aatttcctga gggcctgcga agacgttggg ggttttctct
     2281 cgaaggtgtt caaaaagagc gccttcaccg acgagctcgt tgccgggatc gacaggctga
     2341 ccgcactcgc gaggcgtatc gcgcgttccg tgaattccga gggttacctc ggacagcgcg
     2401 tcctacgagt tgtcacgcat acgggccgat ctcttcatga acagggagag gatcaggaaa
     2461 cagcttgaac gcataatggg ccgggaggcc atgcgtccca tcatccagga tgactatata
     2521 tccctgcgga acaacaggta cgtcattccc ctgaagccga acttcaacga ggccgtccag
     2581 ggcatcgtgc acgactattc ccattcgctc aagacctcct ttgtcgagcc cgtggagtgc
     2641 gtggaactca acaacagcat caacatcctc atcaacgagg aaaaggagga ggaaaagaag
     2701 gtcctccgcg agcttaccga tttcacgcgg ggcttcaggg aggccctgga aaggaacgta
     2761 cggtgcctcg ccgagctcga cttctttcac gccatggcgc tcttctgcca tgaattcagg
     2821 tgtgtcaggc ccgaggtgac gctgtcggga agccttgagg tgaagaaggc cgtcaatcca
     2881 ttcatagcga tgtcccgcaa gagcgaagcg gtgcccatcg acatcgttat gaaggagagc
     2941 gagaaggtga tgatcatcag cggtcccaac gccgggggca agaccgcagc gctcaagacg
     3001 atcggcctgc tctcgctaat ggcgaagaca ggccttttca ttcccgccac cgaaagaccg
     3061 gtggttcccc tcttctcgaa tgtcttcgcg ctgatcggcg acgagcagga catatcgctg
     3121 gaactgagca gcttcactgc ccacatgtat gccattaagg acctctatct caacgccggg
     3181 ggtgacgaac tcgttctcat tgacgagata ggg
//
LOCUS       DAOPOA010000193         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_32642_length_3210_cov_3.984469, whole genome shotgun sequence.
ACCESSION   DAOPOA010000193 DAOPOA010000000
VERSION     DAOPOA010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32642_length_3210_cov_3.984469"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1125)
                     /locus_tag="PKJ17_05105"
     CDS             complement(100..1125)
                     /locus_tag="PKJ17_05105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOD75382.1"
                     /translation="MPMFEKNWQELIRPTKIEIEKKESTYVKFSTEPFERGYGVTIGN
                     SLRRVLLSSIMGAAITSVWIDSVDHEFSAVRGVKEDVTEMILNLKKVVIRMTDDKPAL
                     LKIDVKGKREVKAGDITHDGGVDVINPDLHIATLSSDAKLYMEMKARLGRGYQPSEQN
                     VDDADPIGTIPIDAIFSPIRKVSYNVTQARVGRRTDYDKLSMEIWTDGSVDPLDALSF
                     AAKIVKEQMRIFINFDEVEEAEAGEERGQEKPDFNENLYRSVDELELSVRSANCLKNA
                     DIKYIGELVQKTEQEILMTKNFGRKSLNEIKEILSCMGLRLGSKLDSFPAREELDKMV
                     LKKKDHM"
     gene            complement(1147..1767)
                     /gene="rpsD"
                     /locus_tag="PKJ17_05110"
     CDS             complement(1147..1767)
                     /gene="rpsD"
                     /locus_tag="PKJ17_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOD75383.1"
                     /translation="MSRYVGPSCKLCRREGIKLFLKGERCYTEKCAIEKRNYPPGQHV
                     DTKGKVMEYGMRLREKQRARRIYGLSEKQFRRFFVMAERKEGITGTNFLVFLERRIDN
                     MVFRLGFATSRAEARQLVSHKHIAVNGKTVNSSSYLVRVGDVVEVRHKSLPAVVNALE
                     SVVRRGVPSWIDLDKDNMKGTIRLFPTRDDISLPIKEQLIVEYYSR"
     gene            complement(1780..2169)
                     /gene="rpsK"
                     /locus_tag="PKJ17_05115"
     CDS             complement(1780..2169)
                     /gene="rpsK"
                     /locus_tag="PKJ17_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOD75384.1"
                     /translation="MAKVRRSGKKKVKKNIPVGGAYIQSSFNNTIITITDPQGNVVSW
                     SSGGVVGFKGSRKSTPFAAQLAAENAAKKAMEHGLRTVDVYVKGPGAGREAALRALQS
                     AGLKIHLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(2188..2553)
                     /gene="rpsM"
                     /locus_tag="PKJ17_05120"
     CDS             complement(2188..2553)
                     /gene="rpsM"
                     /locus_tag="PKJ17_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOD75385.1"
                     /translation="MARIAGVDIPRDKRIEVALTYIYGIGRTLSVKILNQAGIDPNKR
                     THALLDEEIAKIRDILENEYKVEGDLRKEVSMNIKRLMDIGCYRGLRHRRSLPVHGQR
                     TRTNSRTRKGPRKTSMKRK"
     gene            complement(2565..2678)
                     /gene="rpmJ"
                     /locus_tag="PKJ17_05125"
     CDS             complement(2565..2678)
                     /gene="rpmJ"
                     /locus_tag="PKJ17_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOD75386.1"
                     /translation="MKVKPSVKKRCDKCKIIKRKGVVRIICENPKHKQKQG"
     gene            complement(2688..2906)
                     /gene="infA"
                     /locus_tag="PKJ17_05130"
     CDS             complement(2688..2906)
                     /gene="infA"
                     /locus_tag="PKJ17_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOD75387.1"
                     /translation="MPKGEGIEIEGTVVEPLPNAMFRVELPNGHRVLAHVSGKMRMHY
                     IKILRGDKVVVELSPYDLTRGRIIYRVK"
     gene            complement(2916..>3210)
                     /locus_tag="PKJ17_05135"
     CDS             complement(2916..>3210)
                     /locus_tag="PKJ17_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOD75388.1"
                     /translation="ECVEGERFSVVREFVGHGIGRSLHEEPQVPNYGTKGTGLRLKAG
                     MVLAIEPMVNMGKSEVYIKENGWTAATKDGSLSAHFEHTVAVTGDGPVILSRA"
BASE COUNT          575 a          972 c          762 g          901 t
ORIGIN      
        1 cgaacagggc gttggcgagg ttcatgaaca acgcccgctt gtggctcttg gttcgattca
       61 ttttcttcac tctgttctga tgcctcatta tatcaacccc tacatgtgat cttttttctt
      121 gagaaccatc ttgtccagtt cttcccttgc cggaaagctg tctaattttg aaccaagccg
      181 aaggcccatg caagaaagaa tctccttgat ctcgttcaaa gacttccttc cgaagttctt
      241 tgtcatgagg atctcctgct ccgtcttctg gacaagttct cctatgtact tgatatccgc
      301 gttcttcagg cagttcgcgc ttctcacgga gagttcgagt tcgtcgacgc tgcgatacag
      361 gttttcgttg aaatcgggtt tttcctgtcc tctttcctcg cccgcctcag cttcctcgac
      421 ctcgtcgaag ttgatgaaga tccgcatctg ttccttgacg atctttgccg cgaaggagag
      481 ggcatcgagg ggatcgacgc tgccgtcggt ccagatctcc atggacagtt tgtcataatc
      541 cgtccgccgt cccacccggg cctgggtcac gttatagctc accttcctga tgggcgagaa
      601 aatggcgtct atgggaattg tgccgatggg atcggcgtca tcgacgttct gttccgaagg
      661 ctgataaccg cggccaagcc ttgccttcat ctccatatac agcttggcgt cgcttgacag
      721 tgtggctata tgcagatcgg gattgatgac gtccaccccg ccgtcatgtg tgatgtctcc
      781 cgctttcact tccctcttgc ctttgacatc gatcttcaaa agggcaggtt tgtcgtcggt
      841 catcctgata acgaccttct tcaggttcag gatcatctcc gtaacatctt ccttgacgcc
      901 ccggaccgca gagaattcgt ggtcaacgga gtctatccag accgacgtta tagcggcacc
      961 catgatcgag gacaggagca cccgccggag agagttaccg atcgtgacgc cgtaccctct
     1021 ctcgaagggt tccgtggaga acttcacgta ggtgctctcc tttttctcta tctcgatctt
     1081 ggttggcctg ataagctcct gccagttctt ttcaaacata ggcatgtacc ctccttgggg
     1141 taataactac cttgaatagt actctacgat cagctgttcc ttgatcggca acgaaatatc
     1201 gtcacgggtc ggaaagagcc tgatggtacc cttcatgtta tccttgtcga ggtctatcca
     1261 tgagggcacg ccccttcgaa cgaccgattc cagcgcattc acgacggcag gcaggctctt
     1321 gtgcctgacc tctacgacat cgcccactct cacgaggtag gacgaggagt tcactgtctt
     1381 gccgttcacg gcgatgtgct tgtgggacac aagctggcgc gcctcggcac gggacgtggc
     1441 gaagcccagg cggaagacca tgttgtcgat ccttctttca agaaaaacga ggaagttggt
     1501 gcccgtgatg ccttccttgc gctcggccat gacgaagaac cgtctgaact gcttctcgct
     1561 gagcccgtat atcctgcgcg cgcgctgttt ctcccgcaga cgcatcccgt attccatgac
     1621 cttacccttg gtgtccacgt gctgtcccgg ggggtagttc ctcttttcga tggcacactt
     1681 ctccgtgtag catctttctc cctttaggaa aagctttatg ccttctcgcc ggcataactt
     1741 gcatgatggt ccaacatatc gtgacaattc gtgcctcctt taaacccttc tgcgtttcgg
     1801 cggtctgcag ccgttgtggg ggatgggcgt aacgtcgcgg atgagatgga tcttcagtcc
     1861 cgcgctctgc aaagcccgta acgccgcctc gcgtcccgca cccgggccct tcacgtagac
     1921 atccaccgtc ctgagcccat gctccatggc cttcttcgcg gcgttctctg cggcaagctg
     1981 cgcggcaaaa ggagtgctct tgcgcgagcc cttgaaaccc acaacccctc cgcttgacca
     2041 cgacacgacg ttgccctgcg gatcggtgat cgtgatgatc gtgttgttga agctcgactg
     2101 tatatatgct ccaccgacgg ggatattctt ttttaccttc ttctttccgc ttcttctgac
     2161 cttagccatg tacgcaccac ctcgtcgtta ttttcgtttc atcgaagtct tccgcgggcc
     2221 tttgcgggtc cgggagttcg tcctcgtcct ctgcccgtgc actggaaggc tccgtctatg
     2281 tctcaggccg cgatagcatc cgatgtccat gagccttttt atgttcatgc tcacttcttt
     2341 tctcagatca ccttcgacct tgtattcgtt ttccaggatg tcccttatct tcgcgatctc
     2401 ttcgtcgagg agggcatgcg tcctcttgtt cggatcgatc ccggcctggt tcaggatctt
     2461 cacggaaagc gttctcccga tgccgtaaat gtaggtcagg gccacttcga tccttttgtc
     2521 cctgggtatg tcgacgcctg caattcgtgc caccttttgc ctccttagcc ctgtttctgt
     2581 ttatgcttgg ggttctcaca gatgatcctg acaacaccct ttcgcttgat gatcttgcat
     2641 ttgtcacatc tcttcttcac cgaaggcttt accttcatgc tttcctccta cttgacgcga
     2701 taaatgatcc tgccccgcgt gaggtcgtat ggcgacaact cgaccacgac cttgtctccc
     2761 ctgagtatct ttatgtagtg catccgcatc tttccagaca cgtgggccaa taccctgtgg
     2821 ccgttgggga gctcaactct gaacatcgcg ttgggcaatg gttctacgac cgttccttca
     2881 atctctatac cttcgccttt tggcatttta cctctctagg cccggctcaa tataacgggt
     2941 ccgtcgccgg tcacggccac cgtgtgttca aaatgagccg acaggcttcc gtccttcgtg
     3001 gccgctgtcc aaccgttctc cttgatatac acttcactct ttcccatgtt caccatcggt
     3061 tctatggcaa gtaccattcc agccttgagc ctgagacccg ttcccttcgt accgtaattg
     3121 ggcacctgcg gttcttcatg caggctccgt cctattccgt gaccgacaaa ctcccttacg
     3181 acggagaacc tctccccctc gacacactcc
//
LOCUS       DAOPOA010000194         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_33047_length_3182_cov_2.996482, whole genome shotgun sequence.
ACCESSION   DAOPOA010000194 DAOPOA010000000
VERSION     DAOPOA010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33047_length_3182_cov_2.996482"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PKJ17_05140"
     CDS             complement(<1..898)
                     /locus_tag="PKJ17_05140"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HOD75389.1"
                     /translation="MNGAELVLKTAVLNGIDICFANAGTTEIPLVAALDGQAGIKTVL
                     GLFEGVCTGAADGFGRVLDRPAMALLHLGPGFANGIACLHNARRAKAPVVNVIGEHAS
                     WHRDADPPLCMDIAAMVGAVSGWKRTCRSARGLSRDMAAVIAASSRGQVASLIIPNDH
                     QWAECGGIPIVTASPSFEPVDHEVVDRAAKALKTGGRTAVVLGGRALRHEGLMTAARI
                     RAATGCDLLANSFPGYMDRGGSLPVVTRIPYFPEPALELMTRYDAVVLAGAWEPVTFF
                     GYEGVPSHVIPGGTMKIAIHGPG"
     gene            1337..1774
                     /locus_tag="PKJ17_05145"
     CDS             1337..1774
                     /locus_tag="PKJ17_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD75390.1"
                     /translation="MSQRQNTREELLAEIAGLRQRIVELETTQTEFSQIEATLRKTEE
                     KYRSIFEDAMEGIFQTSVDGRFLSANPSLSRVHGYDSPQDLIESITNISKQLYVNPED
                     RARTMEKIEREGSVRNFETQMYSKDGSIHWISMNIQPSAISST"
     gene            1771..>3182
                     /locus_tag="PKJ17_05150"
     CDS             1771..>3182
                     /locus_tag="PKJ17_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD75391.1"
                     /translation="MIRYYEGTMLDITERKKAEAALQESEERYRTAIENSNDGVAIIQ
                     GYTLQYVNRRFLEMFEFPSTEEAIGESVLTTIHPDDLSRVRDINQRRQRGEPVPSRYE
                     FKGITRNGRTIYVEVSAATIPYRNATVYLVYLRDVTERKEAEEALIQSRNALESLNRA
                     KTKAVNHISHELKTPLSVIQGSMRLIRRKLEPTAQYVSIRDLVDSIERNLQRLFNISR
                     ETDQIFRLSQELEAVMLVGDLDRLWQRMESLSEVPADVRGHWNSLREWIGQYFAASTQ
                     FFQSIDLYTFVVSVVERMKARLPGRDLDIRVDGANDLFISMDPQILRDIAEGLIKNAI
                     ENTPDGGSIRIGLEQKDDRIVLSVTDTGVGITEENKRFLLDGLHHAKETDLYSSRKPL
                     EFGAGGKGLDLLRIKYYSLRFGFDISFESNRCVHIPTDRDLCPGNISKCPAIRGTEEC
                     LASGGTTFTVSFPVRKRSASV"
BASE COUNT          724 a          992 c          879 g          587 t
ORIGIN      
        1 gcccgggacc gtggatcgcg atcttcatgg tccccccggg aatgacgtga ctggggacgc
       61 cctcatagcc gaaaaaagtg acgggctccc aggcacccgc caggacgacc gcgtcatacc
      121 gcgtcatcag ttccagcgcc ggttccggaa agtaggggat cctcgtcacc acgggcaggg
      181 acccgccgcg gtccatgtac cccggaaaac tgttggccag aaggtcgcat cccgttgccg
      241 cgcggatgcg cgcagccgtc atcaacccct cgtgcctcaa cgccctccct ccgaggacca
      301 ctgccgttct cccgcccgtc ttcaaggcct ttgccgcccg gtccacaacc tcatggtcga
      361 caggctcgaa ggagggggag gccgtgacga tgggaatacc cccgcattcg gcccactgat
      421 ggtcattggg gatgatgagg ctcgccacct gtccgcgaga gcttgccgct atgacagccg
      481 ccatgtcgcg ggagaggccc cgggcgctcc tgcacgtcct cttccatccc gaaacggccc
      541 ccaccatcgc cgctatatcc atgcagagcg gcgggtccgc gtcacgatgc caggacgcgt
      601 gctcgccgat gacgttgacg acaggcgcct tcgcgcgccg cgcgttgtga aggcaggcta
      661 tcccgttggc aaagcccgga cccaggtgga gaagggccat cgccggcctg tcgaggaccc
      721 gcccaaaacc atcggccgcg cccgtgcaga ccccctcgaa tagaccgagg acggtcttta
      781 tgcccgcctg accgtcaagg gccgcgacaa ggggtatctc cgtggtccca gcgttggcga
      841 aacagatatc gataccattc aacacagccg tcttcaacac aagttcagcg ccgttcatag
      901 accccccgat gaaacagatg atggtgaaac aggtgatagg tcatgggtga tgggaaagcc
      961 ttgacggccg tacctattac ccatgaccta taacccataa cctgttctat tcttctcagt
     1021 atatatggaa ccgggaggat ctgacaaggc atatcggagt catgatgagg caggttatgg
     1081 tgaaacaggc aatgggtaat gggaaagcct tgaaggccgt acctattacc catgacctat
     1141 aacccataac ctgttttttt gcccctggca cccattttcg tttcttctat ccctgtcgtc
     1201 ttgttatccc cgtccaaaca acttataata gagttgattc aggagacggt aacaggtaaa
     1261 taggtgagag gtgaccgatc atcaccgcct gtcctttccc gtcacccatg agctgtttca
     1321 tcgagaccga ggttccatgt cacaacggca aaacaccaga gaagagcttc ttgcggagat
     1381 cgcgggtctc cggcagcgca ttgttgagct tgagacgacc cagacagagt tcagccagat
     1441 cgaagcgacc ctgcgcaaga cagaggagaa gtatcgcagc atattcgagg atgccatgga
     1501 aggcatcttt cagacaagcg tggacggacg attcttgagc gccaaccctt cgctctcccg
     1561 cgttcacgga tacgattctc cccaggacct gatcgaatcc atcacgaaca tctcgaagca
     1621 actctacgtc aaccccgaag atcgcgcccg gaccatggaa aaaatagaac gggaggggtc
     1681 ggtgcggaac ttcgaaacac agatgtacag caaggacggc agcatccact ggatctccat
     1741 gaacattcag ccgtccgcga taagttcaac atgatccgct actacgaagg cacgatgctg
     1801 gacatcacgg agcgcaagaa ggcggaggcc gccctccagg aaagcgaaga gcgctaccgc
     1861 acggctatcg agaactccaa cgacggtgtg gccatcattc agggctacac cctccaatat
     1921 gtgaacaggc gctttctcga gatgttcgaa ttcccttcca ccgaagaggc cataggcgag
     1981 tcggtcctta ccaccatcca tccagacgat ctctcgagag tgcgggacat caaccagcgc
     2041 cgccagcgcg gggagccggt gccgtcgcga tacgagttca agggcatcac aaggaatggc
     2101 aggacgatct atgtcgaggt ttcagccgcc accattccct atcgcaatgc caccgtctac
     2161 ctcgtctatc tccgggatgt gacggagcgc aaggaggccg aggaggccct catccagtcc
     2221 cgcaacgccc tggagagcct gaaccgcgcc aagacgaagg cggtcaatca catctcccac
     2281 gaactgaaga cgcccctgtc cgtcattcag gggagcatga ggcttataag acgaaaactc
     2341 gaacccacag cccagtacgt ctccatacgg gacctggtcg actccatcga gcgcaatctc
     2401 cagagactct tcaacatctc ccgcgagacg gaccagatat tcaggctttc ccaggaactc
     2461 gaggcggtca tgctcgttgg tgacctcgac cggctgtggc aaaggatgga gagcctctcc
     2521 gaggtgcctg ccgacgtgcg cggccactgg aactccctga gagaatggat cggccagtat
     2581 ttcgcggcta gcacccagtt cttccagtcc atcgaccttt acacctttgt cgtttccgtt
     2641 gttgagcgta tgaaagcccg gctgcccggg cgagatctcg acatcagggt ggacggggcg
     2701 aatgacctct ttatctccat ggacccccag atcctccggg acatcgccga aggcctcatc
     2761 aagaacgcca tcgagaacac gcctgacggg ggtagcataa ggatcggcct cgagcagaag
     2821 gatgaccgga tcgtattgag tgtgacggac accggcgtcg ggatcaccga agagaacaaa
     2881 cgcttcctcc tcgatggttt gcaccacgcg aaggagacgg acctctactc ctcaaggaaa
     2941 ccgctggaat tcggcgccgg cgggaaaggg ctcgacctct tgaggatcaa gtactactcc
     3001 ctgcggttcg gcttcgacat ctccttcgaa agcaaccgct gcgtgcatat ccccacggac
     3061 cgggaccttt gcccggggaa catctcaaaa tgccccgcca tcaggggcac ggaggagtgt
     3121 ctcgcgtcgg gtgggaccac cttcactgtc tccttccccg tcaggaagag atcggcctcc
     3181 gt
//
LOCUS       DAOPOA010000195         3168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_33255_length_3168_cov_4.139415, whole genome shotgun sequence.
ACCESSION   DAOPOA010000195 DAOPOA010000000
VERSION     DAOPOA010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33255_length_3168_cov_4.139415"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKJ17_05155"
     CDS             <1..489
                     /locus_tag="PKJ17_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOD75392.1"
                     /translation="GVTGDVREIGLFATTIDTVDNLCVVVGNNKILSDNIINYTRNPF
                     RRVDLKAQLAHGVDPREAADRLMDRIKAIPNVIDALSPALRSWSSIWPGLSLPSGPSA
                     TTRTTGRSSSTPTKPYRRSAQPRAIRHHRQTRSSTTNPWRHKHPRAADGKKLKRGSRR
                     PA"
     gene            complement(557..766)
                     /locus_tag="PKJ17_05160"
     CDS             complement(557..766)
                     /locus_tag="PKJ17_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75393.1"
                     /translation="MLEMKSDTITVQKGKEKWEIARDPSTKVTGDVKVGSKVTIEYTI
                     KAATIEVKDAGKTAKPKKGESAPGK"
     gene            complement(878..1339)
                     /locus_tag="PKJ17_05165"
     CDS             complement(878..1339)
                     /locus_tag="PKJ17_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75394.1"
                     /translation="MLDRNVCGRIILAIVLIMLPLAAGAGDISGDVEKVLRQILQDRP
                     VPSLDYLQKAEAINTGCAYYRGSYRGIAITVETHPDSDRVASLLLEIPGGDWTREIFP
                     AVRRVIGPERYKRPKQSEYGWEWPDFRAASVHYVKGEKPGEGLTVVSLFYR"
     gene            complement(1397..1708)
                     /locus_tag="PKJ17_05170"
     CDS             complement(1397..1708)
                     /locus_tag="PKJ17_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOD75395.1"
                     /translation="MKDIHETLTILKKEFPRVYEAHEALGKEIHEGSGPIPERTRWLI
                     KIAISGAASHRLSLETHIAKGREAGLTDGEIKQALLLLIPTVGFPTFMEAYSVYLADG
                     G"
     gene            1849..2652
                     /locus_tag="PKJ17_05175"
     CDS             1849..2652
                     /locus_tag="PKJ17_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOD75396.1"
                     /translation="MKRQVMKIGRHLAVVIGVLALLVLPGGFLAWADTNGLDQALLEA
                     SAKGDLAVVRSLLEKGADVNARTDMGETALHLASSEEVVRVLIEHGADVNATDAEYGM
                     TPLFNQRIAVARLLVEKGADVNARSKKGVTPLMWAVYWDAVDKAGFLVEKGADVNARD
                     EHMRTPLHVAANWNKKELADFLLSHRADVNARDASGWTPLHWAAFEGGPEVAEVLLSR
                     GADTTVRSTRDRMIFPAGSTPVDVAKRAKNGGMADYLLNVDRTKEGTTR"
     gene            complement(2704..>3168)
                     /locus_tag="PKJ17_05180"
     CDS             complement(2704..>3168)
                     /locus_tag="PKJ17_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75397.1"
                     /translation="RDSVRSALREAGARGFRSVAFPLIGAGTGGGSEADVEGFIEEEC
                     RRSDWDGEVVIVRYRNGKWPPGRCHCDLWKKNPGTLAGQGIPEGFCGLCDVCGEPGHI
                     LHFPGSVPVTRAWCRKHYYRAMILHPGGSIGIFLWALAALAAIGLTVVLVKG"
BASE COUNT          630 a         1011 c          921 g          606 t
ORIGIN      
        1 ggggtcacgg gggacgtgcg cgagatcggg cttttcgcga cgacgatcga caccgtggac
       61 aatctctgcg ttgtggtggg caacaacaag atcctctccg acaacatcat caattatacc
      121 cgcaacccct tccggcgcgt ggacctcaag gcgcaacttg cccacggcgt cgacccccgc
      181 gaggcggcgg accgtctcat ggacaggata aaggctattc ccaacgtgat cgatgccctg
      241 agcccagcgt tgagatcctg gagttcaatc tggccgggcc tgtccttgcc gtcaggccct
      301 tctgcaacaa caaggactac tggcaggtct tcttcgacac caacaaagcc atacaggagg
      361 tctgcgcaac cgcgggctat ccggcaccat cgacaaacca ggtcatctac cacaaacccc
      421 tggcgacata agcacccccg cgccgccgac gggaagaagc ttaagcgggg ctcaaggcgc
      481 cccgcttaag ccagacccct tcagcctgac aatctctatt tcaggcatat cgatctcctg
      541 atcgaggaaa ccgcgactat tttcctggag cggattcgcc ctttttcggt tttgccgtct
      601 tgcccgcgtc cttgacctcg atggtcgccg ccttgatcgt gtactcgatg gtgaccttcg
      661 atcccacctt cacgtcgccc gtcaccttcg ttgatggatc cctggcgatc tcccactttt
      721 ccttaccctt ctggaccgtg atggtgtcgg acttcatctc caacaccggc ccggtcacct
      781 ggtacgtctt gggaccttca caggcccaat tccgggaagc gcttgaggga acgaagtatt
      841 taagataggc aaacatcagg tggtgtccga ggccgcacta cctgtagaaa agagaaacga
      901 ccgtgagccc ttctccgggt ttctcaccct tgacgtagtg aaccgaagca gcacggaagt
      961 cgggccactc ccagccgtat tcggactgtt tcggcctctt gtagcgctcg gggccaatga
     1021 cgcgcctcac ggcggggaat atctccctgg tccagtcccc acccgggatc tcaagcaaga
     1081 gagatgccac gcggtcactg tccggatgcg tctccacggt gatcgcgatg ccccggtagc
     1141 tcccccggta ataagcacag ccggtattga tggcctcggc cttctgcagg tagtcgagtg
     1201 agggtacagg cctgtcctga aggatctgac gaagcacctt ctccacgtcg ccggagatgt
     1261 ccccggcacc ggccgcgaga ggcaacatta tcagaacgat ggcaagaatt atgcgtccac
     1321 agacatttcg atccagcatt gcgcaactcc aggtattgtg atctgccgcc tctacagcct
     1381 catgggatgc cccccgttac ccgccatccg cgagatacac ggagtaggcc tccataaagg
     1441 tggggaagcc caccgtcggt ataagaagga gaagggcctg cttgatctcc ccgtcggtaa
     1501 gaccggcttc cctgcccttt gcgatgtgcg tctccagcga tagacggtgg ctggcggccc
     1561 cggagatggc gatcttgatg agccatcttg tcctctccgg tatggggccg gacccctcgt
     1621 ggatctcttt ccccagtgcc tcgtgggcct cgtatacccg gggaaactct ttcttcagga
     1681 tggtcagggt ctcgtggata tctttcatgg cggtcacctc ctgtacggac ttgaaacggt
     1741 tgacatacac tattctttag acggcgcgac cccttttgtc aagcgcaagc cgtccggccg
     1801 ggcatccttc cctgccggtc ttgacaaaaa ccctggagag gcttcacaat gaagagacag
     1861 gttatgaaga tcggcagaca tttggccgtt gtcatcggcg tcctcgccct cctggtcttg
     1921 ccagggggtt tccttgcgtg ggcggacacg aacggtctcg atcaagccct gctggaagct
     1981 tccgcaaagg gagatctggc ggtagtgagg agccttctgg agaagggcgc ggacgtgaac
     2041 gcgcgcacgg acatgggtga gacggcgctt cacctggcaa gctccgagga agtggtccgc
     2101 gtcctcatcg aacacggcgc ggacgtgaac gcgacggacg ccgagtatgg gatgacgcct
     2161 cttttcaacc agcgaatcgc cgttgcccgg cttctcgtgg agaagggcgc cgacgtgaac
     2221 gcgcgaagca agaagggggt cacgcccctc atgtgggccg tctactggga tgccgtggat
     2281 aaagccgggt tcctcgtcga gaagggcgcc gacgtgaacg cgcgggacga acacatgaga
     2341 accccgctcc atgtcgcggc caactggaac aagaaggaac tggccgattt cctcctgtcc
     2401 cacagggctg acgtgaacgc ccgcgatgcc tccggctgga cccccctcca ctgggcggcc
     2461 ttcgaaggcg ggcccgaggt cgctgaggtc ctcctgtcgc gcggcgcgga cacgacagtg
     2521 cgtagcaccc gggaccggat gatctttccc gctgggtcta cccctgtcga cgtcgcgaaa
     2581 agggcaaaga acgggggaat ggcagactat ctcttgaacg ttgacaggac gaaggagggc
     2641 acgacgaggt gagccgcgag atctccggcc gccgcaggcg gcactcccca gccggaagtt
     2701 ttgctatccc ttgacgagca ctactgtgag cccgatcgcg gcaagcgcgg caagcgccca
     2761 caggaagatg cctatcgacc cgccgggatg gagtatcatg gccctgtagt agtgtttcct
     2821 gcaccaggcg cgagtgaccg ggacagagcc gggaaagtgc aggatgtggc ccggctcacc
     2881 gcagacgtca cagagcccgc aaaatccttc gggaatgccc tggcctgcca gcgtccccgg
     2941 gttctttttc cacaggtcgc agtggcagcg ccccggtggc cactttccat tcctgtagcg
     3001 cacgatgacc acctccccat cccagtccga gcgacggcat tcctcctcta tgaacccctc
     3061 aacatcggcc tcggaacctc caccggtgcc cgccccgatc aagggaaaag cgactgagcg
     3121 gaatcctctc gcccccgcct ccctcaacgc ggaacggacg gaatcccg
//
LOCUS       DAOPOA010000196         3161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_33357_length_3161_cov_3.040245, whole genome shotgun sequence.
ACCESSION   DAOPOA010000196 DAOPOA010000000
VERSION     DAOPOA010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33357_length_3161_cov_3.040245"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PKJ17_05185"
     CDS             complement(<1..356)
                     /locus_tag="PKJ17_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75398.1"
                     /translation="MTPDELIARLRPHADALRKGAKSSIAGLGKVLGFNVLEGVIIPK
                     KCLCAAIGKGHVSVACGTRFLSRPRVVGFRRFSFEEGRYPRPEELASAVLSARDAFRA
                     RDAAMILSVPRPWVLT"
     gene            complement(353..1309)
                     /locus_tag="PKJ17_05190"
     CDS             complement(353..1309)
                     /locus_tag="PKJ17_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOD75399.1"
                     /translation="MIRSRKGIALLMVLWVITMLMALVMSFSLLTRTEARSTIFFKEG
                     TQKKFLAEAGVERAVMEIYHRQTFRNQAVAIEGNEVVRIDGRQYSARIGQGSYNFRLL
                     SESGKIDINKMTDLTGIVLKNLLVNTGMTDEEAAVIVDSILDWADKDNLRRLNGAEDE
                     YYLSLPVPYRAKNRPFDAIEELLLVKGMSVDILLGTGERRGIIHYLTIYGTSSKVNIN
                     AAPKEVLIALPGLTEETVTRILDQREAAEFTSVQEIQAITGLNFPTLAQYIDMSETDV
                     YTIESVGFQGDSKDGYGIRAIVSVPGTVPRFLYYKTPSEMHR"
     gene            complement(1306..1938)
                     /locus_tag="PKJ17_05195"
     CDS             complement(1306..1938)
                     /locus_tag="PKJ17_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD75400.1"
                     /translation="MTCRRLRHDLEGRGQGRGFTLLEVMISITLISILVVIIAGAMRL
                     ANRSVERGERRSEELERFKVSFLVMDAQIQSAIPLVRADDEAGRLFFEGSGDTVKFAS
                     NYSLTGGYRGYVTVTYRVRPNGEGRNSLYIEENTIGLENTREMRLLDGLDEIRFEYFR
                     KENDADDTTGEWVETWTDELLFPRRIRVHVARAGQKIQLTVPVRARKAAS"
     gene            complement(1935..2342)
                     /locus_tag="PKJ17_05200"
     CDS             complement(1935..2342)
                     /locus_tag="PKJ17_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOD75401.1"
                     /translation="MENLLGKERGFTLLEVIVAFSLLAVTVTVILQLFSSNLRVLSTS
                     EDYASAVVRAEAKMRDVLAGEDITEGVSSETTPEGYRVETTVSKVYERRTRDLPFEVL
                     EIDVRLTWKSLVAETSMTLRTLKTVKRQQAGRI"
     gene            complement(2378..2821)
                     /locus_tag="PKJ17_05205"
     CDS             complement(2378..2821)
                     /locus_tag="PKJ17_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD75402.1"
                     /translation="MGFPSAGGFTLLEIMMVMVLVLIMLGISTVLFSSMLPSQNLNAA
                     GRELSAMLRFARLLATNTGEPRTVFVDLETGRYGIQGVQARRIPQGITVKVTDVLEGE
                     ITRGRYSIIFNEAGGVPWGRITLSGGKRVLTIELDPVVGAVVLKQ"
     gene            complement(2848..>3161)
                     /locus_tag="PKJ17_05210"
     CDS             complement(2848..>3161)
                     /locus_tag="PKJ17_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOD75403.1"
                     /translation="IIAGSVESIARNVREGRGIAGPLMEAGVFPQLALSMIKVGEETG
                     QLDSMLLKVAATYEKALRDAVKRFMSLLEPAIILSMGIIIAFIVVSMLMAVFSILELP
                     F"
BASE COUNT          600 a         1015 c          869 g          677 t
ORIGIN      
        1 ctcgtcagga cccatggccg ggggacggag aggatcatcg ccgcgtcacg cgccctgaaa
       61 gcgtcccggg cggagaggac cgccgatgcc agctcttccg ggcggggata ccttccttcc
      121 tcgaaactga agcgtctgaa ccccacgact ctgggtcgcg acaggaatcg tgttccgcag
      181 gcgaccgaga catgaccctt cccgatggcg gcgcagagac actttttcgg gatgatgacg
      241 ccctcgagga cattgaagcc aaggaccttt ccgaggcccg cgatagacga cttcgccccc
      301 tttctcaggg cgtcggcgtg cggcctcaac cgtgcaatca attcatcggg cgtcatcggt
      361 gcatctccga aggcgtcttg tagtacagga accggggtac cgtgcccggc acggacacga
      421 tggcccggat cccgtaccca tctttcgaat ctccctgaaa acccacggat tctatcgtgt
      481 agacatctgt ctcgctcatg tctatgtatt gcgcaagggt ggggaaattg agtcccgtta
      541 tggcctggat ctcctggacg gacgtgaact cggccgcctc ccgctggtcc aggatcctcg
      601 tgacggtctc ctccgtcagg cccggcaggg ctatgaggac ctctttcggg gccgcattga
      661 tgttcacctt cgaggacgtc ccgtatatcg tcaggtaatg gatgatcccc ctgcgttccc
      721 cggtcccgag gaggatatcg acggacatac ccttcaccag caggagctcc tcgatggcgt
      781 cgaagggcct gttcttggcc ctgtagggaa cggggagaga aaggtagtac tcgtcttccg
      841 ctccgttcag gcgccggagg ttgtccttgt ccgcccaatc gagaatggag tcgacgatga
      901 cggcggcctc ctcgtcggtc attcccgtgt tgactagaag gttcttcaaa acgatgccgg
      961 tgagatcggt catcttgttg atgtcgatct tgcccgattc ggaaaggagc ctgaaattgt
     1021 agctgccctg cccgatccgg gcgctgtact gcctgccgtc gatcctcacc acctcgttcc
     1081 cttcgatggc cacggcctgg ttccggaagg tctgcctgtg atagatctcc atgacggccc
     1141 tctccacccc ggcctcggca aggaacttct tctgcgtccc ttccttgaag aagatcgttg
     1201 aacgggcctc agtcctcgtc aggagagaga aggacatcac aagggccatg agcatcgtga
     1261 tcacccacag gaccatgaga agggctatgc ctttccggga gcgtatcatg aggccgcctt
     1321 cctggccctc accggcacgg tcagctggat cttctgacct gcccgggcga cgtggacccg
     1381 gatcctccgg gggaagagca gttcgtcggt ccacgtttcg acccattccc ccgtggtatc
     1441 gtcggcgtcg ttctccttgc ggaaatactc gaagcgtatc tcatccagcc catcgagaag
     1501 cctcatctcc ctggtatttt ccagccctat ggtgttctcc tcgatgtaca gggaattcct
     1561 gccttccccg ttcggcctca cccggtaggt cacggtgaca taccccctgt atcctcccgt
     1621 cagcgaatag ttcgaggcaa atttcacagt gtctcccgac ccttcgaaaa acaggcggcc
     1681 cgcttcatcg tcagccctga cgaggggtat tgccgattgt atctgcgcat ccatgacgag
     1741 gaaagagacc ttgaaccgtt cgagttcctc cgacctgcgc tcgccccttt cgacggaacg
     1801 gttggcgagg cgcatggccc cggcaatgat caccacgagg atgctgataa gggtgatgga
     1861 gatcatgacc tcgaggagtg tgaagccacg accctgcccc ctgccttcca ggtcatgtct
     1921 cagacgccga caggtcatat ccttcccgcc tgctgtcttt tgacggtctt caaggtcctc
     1981 aacgtcatgg atgtctccgc cacgaggctt ttccaggtca gtctcacgtc tatctccaac
     2041 acctcgaagg gcagatcccg tgtccgtctc tcgtagacct tcgacaccgt ggtttccacg
     2101 cgatatcctt cgggcgtcgt ttcactcgac acaccctcgg tgatgtcctc cccggcgaga
     2161 acgtcccgca tcttcgcttc agcccgcacc accgccgacg cgtagtcctc cgatgtcgag
     2221 agcaccctca gattggagga gaaaagctgc agtatcaccg ttacggtcac cgcgagaaga
     2281 gagaaggcga cgatgacctc cagaagtgtg aaaccccgtt cttttccgag cagattctcc
     2341 atttcccctt tctgtcccgt ctggcttccc tcatggttca ttgcttcagg accaccgccc
     2401 ccacaacggg gtcgagttcg atggtgagga cccttttgcc cccggacagg gttatgcgtc
     2461 cccacggcac cccaccggcc tcgttgaaga tgatcgagta cctcccccgg gttatctcgc
     2521 cttccaggac atcggtgacc ttcaccgtga tgccctgcgg tatgcgcctt gcctgcacgc
     2581 cctggatacc gtagcggccg gtttcgagat caacgaagac cgtcctgggc tcgccggtgt
     2641 tcgtcgccag caatctcgcg aaacgcagca tcgccgacag ctccctcccc gcggcgttca
     2701 ggttctggga ggggagcatg ctggagaaga gcacggtcga gataccgagc atgatgagaa
     2761 cgaggaccat caccatcatg atctcgagga gcgtgaaccc gccggcggac gggaacccca
     2821 ttcttccgtt gctgtgcgcc atagctctca aaagggcaac tccaggatgc tgaacacggc
     2881 catcagcatc gatacgacga tgaaggcgat gatgataccc atgctcagga ttattgccgg
     2941 ttcgagaagg ctcatgaacc tcttcacggc atccctgagc gccttttcat aggtggcggc
     3001 caccttgagc agcatggagt cgagctgccc cgtctcctcc ccgaccttga tcatggaaag
     3061 agcaagctga ggaaagacac cggcctccat gagcggaccg gctattcccc tgccttccct
     3121 gacgttcctt gcgatagatt ccacggaccc cgctatgatc c
//
LOCUS       DAOPOA010000197         3156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_33427_length_3156_cov_3.909384, whole genome shotgun sequence.
ACCESSION   DAOPOA010000197 DAOPOA010000000
VERSION     DAOPOA010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33427_length_3156_cov_3.909384"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(389..1411)
                     /locus_tag="PKJ17_05215"
     CDS             complement(389..1411)
                     /locus_tag="PKJ17_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HOD75404.1"
                     /translation="MREDIIPRSCVAEVFFLPSVEISCIFTRNGKTRKAHCIVLFDDM
                     ESALRLQNRLSKVGNISSDGRPILGLDAKRLLAMVIDDCPRALFIPAHVWTPHFSVLG
                     SASGFDSLEECFEELTPRVHAVETGLSSDPPMNWRVSPLDRLTLVSNSDAHSPQKIGR
                     EANILNTDLSFEGIARAVTTREGFDGTIEFFPEEGKYHYDGHRPCKVRMTPRQAADAE
                     YRCPVCGKKLTLGVLNRVEALADREAGFRPKAAKPFTSLIPLREIIADTLRAGAQTKK
                     VDKVYFDLLEKLGPELAILMDAPLTDITRTGHPDIADAIARVRAGDVTIEPGYDGEYG
                     RVAIRR"
     gene            complement(1408..1617)
                     /locus_tag="PKJ17_05220"
     CDS             complement(1408..1617)
                     /locus_tag="PKJ17_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75405.1"
                     /translation="MPFIADLHMHSHYSIATSRDMTPEGLWKWAQLKGVTVIGTGDAI
                     HPGWFRELARKLDPAGNGLLRCAPT"
     gene            complement(1737..2207)
                     /locus_tag="PKJ17_05225"
     CDS             complement(1737..2207)
                     /locus_tag="PKJ17_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOD75406.1"
                     /translation="MRATKKEIRDPAVLAGLLRHCPVGRLGTVGADGSPMIKPLSFVY
                     LDGDIYFHSAREGEKMDDVKRDARVCFEIDLPAGYVRSDASPCRAGYRYQSVIVKGRA
                     LVVEDRETRLRALRALMEKYQPEGGYGPFPEDKLALTAVVRIAIDHISGKQDLR"
     gene            2616..>3156
                     /locus_tag="PKJ17_05230"
     CDS             2616..>3156
                     /locus_tag="PKJ17_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD75407.1"
                     /translation="MSKDEKKEEKVSGISRRNFITGTGVAMAGALATGALLPGKVEAV
                     PPPKKWGRTTDVLIIGTGYSGLAAAIEAHDAGCKVAIIEKAVVIGGNSAIASGIYNCS
                     EPDVQAKYGIKDSPEQHYQQTLAAGDFRGDPEKVKYMTYHALEGRKWLEKQGVQFDEK
                     PYTAVGALWARSFDPVNKGR"
BASE COUNT          638 a          840 c         1048 g          630 t
ORIGIN      
        1 atccggccgt ctttgcatcc cgtctgcgac gggaggcaaa agaaaaggat tcttttcagg
       61 tgggagttat ggggagggga tgggatggga atcttaaggg agtaaggtag tatagggggt
      121 ctggaggatg gttataggac ctatgggaca tataggactt ataggaccta tgactacagg
      181 ggagatgagg ggggattact ccttcaggat aaaaaaacag tatattcttt ctctcccgcc
      241 ggcacggcgg gacgagaagg ccgccggatc ttgggaggct ccgagatccg gcggcagtct
      301 tttctctctt tcctcctctc tctgcgtcct ctgtgagctt gagagaggaa cgcaagcgac
      361 gaacgggcga gagataatgc ctttgcctct aacgccgtat cgcgacccgg ccgtattccc
      421 cgtcgtagcc gggttcgatg gtgacgtcgc cggcccggac gcgggcgatc gcgtcggcta
      481 tatcggggtg gccggtgcgt gtgatgtcgg ttagtggtgc gtccatgagg atggcaagct
      541 cgggcccgag cttctctaaa aggtcgaagt aaaccttgtc caccttcttc gtctgggctc
      601 ccgctcgcag tgtgtcggcg atgatctccc tgagagggat gagagaggtg aaaggtttcg
      661 cagctttcgg gcggaagccg gcctccctgt cggcgagggc ctcgacgcgg ttgagaacgc
      721 ccaacgtgag cttcttcccg cagacagggc agcggtactc ggcgtctgct gcctggcggg
      781 gtgtcatcct caccttgcag gggcgatggc cgtcgtagtg gtacttccct tcctcgggaa
      841 agaactcgat ggtgccgtcg aagccttccc tggtcgtgac tgcgcgagct atcccctcga
      901 acgacaggtc ggtgtttaag atgttcgcct ctcggccgat cttctgcggg gaatgggcgt
      961 cggaattgga gacgagggta aggcggtcga ggggcgagac gcgccagttc atcggagggt
     1021 ccgaagagag gcccgtttcg acggcatgaa cgcggggtgt gagttcctcg aagcattcct
     1081 cgagagagtc aaagcccgag gccgagccaa ggacggagaa gtggggtgtc cacacatggg
     1141 cggggatgaa gagcgcccgg ggacaatcgt cgatcaccat ggcaaggagc ctcttggcgt
     1201 cgaggccgag gataggcctt ccgtcggaag agatgttccc caccttcgaa agccggttct
     1261 ggagacgcag cgcgctctcc atgtcgtcga agaggacaat gcagtgggcc tttcgcgtct
     1321 ttccgttcct ggtgaagatg cagcttatct ccacgctcgg gagaaagaag acctctgcca
     1381 cgcaggagcg gggtataata tcctccctca tgtcggcgcg cagcgcaaca gaccgttgcc
     1441 ggcagggtcg agcttgcgtg caagctccct gaaccagccc gggtgtatcg cgtctcccgt
     1501 gccgatgacg gtcactcctt taagctgcgc ccacttccag agcccctcgg gtgtcatgtc
     1561 ccggctcgtc gcgatggagt aatgggaatg catatgaagg tcggcgataa atggcatcga
     1621 tatcctttcg gtgtcatacg atagcatcga acgggggtgt ttgcaaggcg gtgtcgcggt
     1681 acaggaagga cgtcgggcgg acaggcggcg gatggcattc acgtttcagg agatgctcag
     1741 cggaggtcct gtttgccgga tatgtggtcg atggctatcc tgacgacggc ggtgagcgcc
     1801 agcttgtctt cggggaacgg cccgtagccg ccttcgggct gatacttctc catgagagct
     1861 cggagggcgc gcaatctcgt ctcacggtcc tcaacgacaa gcgctcttcc cttgacgatg
     1921 acgctctggt aacgataacc ggcccggcac gggctcgcgt cgctcctgac atatccggcg
     1981 gggaggtcga tctcgaagca gacccgcgcg tctctcttca cgtcgtccat cttctctccc
     2041 tcgcgggcgg agtgaaagta tatgtcgccg tcgagataga cgaagctgag aggtttgatc
     2101 atgggagacc cgtcggcgcc gacggtgccg agccttccca cggggcagtg cctcaagagg
     2161 ccggcgagga cggccggatc ccttatctcc tttttagttg cgcgcatgtc tgttatctcc
     2221 ctttccttcc tctcttatac cataaccggg cagtgtcatg gagtgtggtg gagacggatg
     2281 ttgttgttaa gtgccccggc catcactatg gggatagttt gtcaaggggg tgcagcgggt
     2341 gatgatggcc ggggccggta ggaccaggaa ctgcgtctgt ctcagtgatc gaaacaaaaa
     2401 gcgtctcaag aatggatccc acccttgaga cgcccctgtc ttacccgcac gtcaacgtgt
     2461 tgaggtgcgg gtgaacccta cggactgtac aagtggatgg aggcccctgc tggtaacagg
     2521 gacgcagcga atcttcgcga aaccgccttg ggcgtgctat aatgaatgtt gctccacaac
     2581 ggaaagacaa catcgatcag caaaaggagg ctctcatgag caaggatgaa aagaaagaag
     2641 agaaagtatc gggcatcagc aggcgtaact tcatcacggg aacaggcgtg gccatggcgg
     2701 gagccctggc gacgggagcg ttgctgccgg gcaaggtgga ggcggtgccg cctccgaaga
     2761 aatggggcag gacaacggat gtcctcatta tcggaacagg ctactccggt cttgccgcgg
     2821 ccatcgaggc ccacgatgcc gggtgcaagg tcgccatcat cgagaaggcg gtcgtgatcg
     2881 gcggcaattc cgcgatcgcg agcgggatct acaactgttc ggagcccgac gtgcaggcga
     2941 agtacgggat aaaggattcc ccggagcagc actatcagca gacgctggcg gccggcgact
     3001 tcaggggcga cccggagaag gtgaaataca tgacgtatca cgccctcgag ggacgcaaat
     3061 ggctggaaaa gcagggggtg cagttcgacg agaaacccta tacggccgtc ggagccctct
     3121 gggcgaggag cttcgacccc gtcaacaagg gaaggg
//
LOCUS       DAOPOA010000198         3153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_33464_length_3153_cov_3.133957, whole genome shotgun sequence.
ACCESSION   DAOPOA010000198 DAOPOA010000000
VERSION     DAOPOA010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33464_length_3153_cov_3.133957"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PKJ17_05235"
     CDS             complement(<1..603)
                     /locus_tag="PKJ17_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD75408.1"
                     /translation="MMDAQKGRGVIGRLRAMSRRKKVVAAGIVLVCVVVIVYVATRKK
                     PAPPPERVAVTAAKAVAKDMPVELREIGTIEGYRSVPIYAQITGQLVKTHFKEGQDVK
                     KGQPLFTIDPNQYQAKVRQAEAQLARDTAQMKFARDEAKRYKYLYEKGAVALSDYENK
                     QSVADAQESLVVADRASLQDARLNLAHCFISAPFDGRMGAY"
     gene            complement(600..1898)
                     /locus_tag="PKJ17_05240"
     CDS             complement(600..1898)
                     /locus_tag="PKJ17_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD75409.1"
                     /translation="MRRYLFCVLAVFLMIVGSDLFAQEAPIRPGEPLNLERAIDIGLK
                     MNPAIMGFQYTMKAREAQLGQARAGYLPKVDGSAGFTRNFEVNNTRDPYYGALLDQYN
                     QNVANVSLKQMIFDFWRTPTSVNIGRLNVESSRRDIDNSMNTVANTVKSSYYGVLKAK
                     RSRDVNLEVVGQYAKHYRVAVVFFEAGKKPKYDVTKAELDLSNAKLNLIGAENDLKVA
                     WVNLNNAMGIDGDADYAIHDSLDFDRYEISLEDALKTAYETRPDLKSLEAQRESAEKA
                     VARAKQEYYPQINGNAQYNFAGSQYPLGQGWYAGVALSVNLFDGLSTTNKVAEAQANK
                     QSVDAKISAMKLQITLDVKQAWLALIKARQTIETTNVQIRQATENLEIANLRYDAGLA
                     TPLEVTDATVTYSQAKLANISALFDYKVARANMEKAMGSR"
     gene            2165..2926
                     /locus_tag="PKJ17_05245"
     CDS             2165..2926
                     /locus_tag="PKJ17_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOD75410.1"
                     /translation="MDMRFKERFITLWEKYFPGAPLPVIFFYSDDEAGATVVKGPKSL
                     EEHRCVLADITRAARGRSLAFNVDSIGCGGGRKYLGFTEVLRPNFEYFLSCGIPGELE
                     GERYKKSPELVAEVMKKMPKFSAPARYIVFKRWDGIDKNDNPDAVIFFATPDVISGLF
                     TLANYDGVDPNGVFCPFGAGCSTIVTYPYLERNARRPKSVIGMFDVSARPCVPANTLT
                     FATPFKRFVTMVDNMDESFLITESWKKVKRRIKKG"
BASE COUNT          614 a          938 c          895 g          706 t
ORIGIN      
        1 ataggcgccc atgcgcccat cgaagggagc ggatatgaaa cagtgggcca ggttgagccg
       61 ggcatcctgc aaagaggccc tgtccgcgac aacgagggac tcctgcgcat cggcgacgga
      121 ctgcttgttc tcgtagtcgg aaagagcgac cgcgcccttt tcgtagaggt acttgtaccg
      181 cttcgcctcg tcgcgggcga atttcatctg cgccgtgtcc cgcgccagct gcgcctcggc
      241 ctggcgcacc tttgcctggt actggttggg atcgatcgtg aaaagaggct ggcccttttt
      301 gacgtcctga ccttctttga aatgggtctt caccagttgc cccgttatct gggcgtagat
      361 gggaacgctg cgataacctt cgatggtccc tatttccctc agctccacgg gcatatcctt
      421 cgcaacagcc ttcgccgcgg tcacggccac ccgctccggc ggcggcgccg gcttctttct
      481 cgtcgcgaca tatacgatga cgaccacgca gacgaggacg atccccgccg ctacgacctt
      541 tttcctccgg gacatcgccc gaagcctgcc gatgactccc ctgcccttct gtgcgtccat
      601 catctgctcc ccatggcctt ctccatgttg gccctggcaa ccttgtaatc gaagagggcg
      661 cttatgttgg ccagcttcgc ctggctgtag gtcaccgtgg cgtctgtgac ctcgagaggg
      721 gtcgcgagtc cggcgtcata gcgcaggttc gcgatctcca ggttctccgt cgcctgccgt
      781 atctggacat ttgtggtctc tatcgtctgc cgtgccttga taagcgccag ccacgcctgc
      841 ttcacatcga gcgtgatctg gagcttcatg gcgctgatct tcgcgtcgac ggactgcttg
      901 ttcgcctggg cctcggcgac cttgttcgtc gtcgacagac cgtcaaagag gttgaccgac
      961 agagcgaccc cggcatacca gccctgcccg agggggtact ggctgccggc aaagttgtac
     1021 tgggcgttac cattgatctg cggatagtat tcctgcttcg cccgggcaac ggccttttcg
     1081 gcggactccc tctgggcctc cagggacttg aggtcgggcc gcgtctcgta ggcggttttc
     1141 agtgcatcct ccagggagat ctcgtacctg tcgaagtcaa gactgtcgtg gatggcatag
     1201 tccgcgtcgc cgtctatgcc catggcgttg ttgaggttca cccaggcgac cttgaggtca
     1261 ttctcggctc cgatgaggtt cagtttcgcg ttggaaaggt cgagttcggc cttcgtaaca
     1321 tcgtacttcg gcttcttgcc cgcctcgaaa aagacgacgg ctacgcggta atgcttcgcg
     1381 tattgcccga cgacctccag gttgacgtcc cgcgaacgct tggccttcag gaccccgtag
     1441 taggaggact tcaccgtgtt ggcaacggtg ttcatgctgt tgtcgatatc gcggcgggac
     1501 gattccacgt tgagcctgcc gatattcaca ctcgttggcg tcctccagaa atcgaagatc
     1561 atctgcttga ggctcacgtt ggcgacattc tggttgtact ggtcgagaag ggccccgtaa
     1621 taagggtcac gggtattgtt cacctcgaag ttgcgcgtga accccgccga gccgtcgacc
     1681 ttcgggagat aacctgcccg cgcctgcccg agctgggcct cgcgcgcctt catcgtatac
     1741 tggaagccca tgatggctgg gttcatctta agaccgatgt ctatggccct ctccaggttg
     1801 aggggctcgc ccggcctgat gggcgcctcc tgggcaaaga ggtcactccc gacgatcatc
     1861 agaaacaccg ccagtacaca gaacaggtat cttctcattt ccagtctact ctcgcctctg
     1921 accggctctt tctcaagcga aaaggggtct gtttgatgaa caggtacgtg acatattaga
     1981 taagtggacc accgttgtca agaaggactt tggacaccgg aagaaacgtg atagaatgga
     2041 aaacggtttt aagttttaag tgttaagttt taagtgccag gatctgcatg aatcaccctt
     2101 gcttcggctt aaaacctaaa acctaaaact taaaaccgtc ttctaaaaaa ggaggaggtc
     2161 attcatggac atgcgattca aggagagatt catcacgttg tgggagaaat actttcccgg
     2221 ggcaccactg ccggtgatct tcttttacag tgatgacgag gccggtgcaa ccgtggtgaa
     2281 agggccgaag agcctggagg agcaccgctg cgtcctcgcc gacatcaccc gggcggcacg
     2341 gggccggtcg ctcgccttca acgtcgattc catcggctgc ggcggcggca ggaaatatct
     2401 cggcttcaca gaggtcctcc gccccaattt cgagtacttc ctctcctgcg gaatacctgg
     2461 agagctcgaa ggcgagagat ataagaagtc ccccgagctg gttgcagagg tgatgaagaa
     2521 gatgccgaaa ttcagcgctc ccgcccgcta catcgtcttc aagcgttggg acgggatcga
     2581 caagaacgac aatcccgacg ccgtcatctt cttcgccact cctgacgtta tttcggggct
     2641 cttcaccctc gcgaactacg acggcgtcga tcccaacggc gtcttctgtc ccttcggtgc
     2701 cggctgctcc accatcgtca cctaccctta cctggaaagg aacgcccgcc ggcccaagtc
     2761 cgtcatcggc atgttcgacg tctccgcccg tccctgcgtc ccggcaaaca ccctcacctt
     2821 cgccacacca ttcaagaggt tcgtaacgat ggtggacaac atggacgaaa gcttcctcat
     2881 aacggagagc tggaagaagg tgaaaaggag aataaagaaa ggctgaacgc gcgcacgggt
     2941 cggcgcttcg cgcgcacggg tcggcgctca cgcgcctaac gagtgaacgg gtataaggat
     3001 aaagaataag gagagataat gatatcgttt actcgtgtgc ccgtttgccc ggagggcaga
     3061 cccgtatgcc cgttaggcgc gtgagcgccg acccgtgtgc gtctctattg aaccccgatg
     3121 tggagcagga ggatcctgcg gacctcttct att
//
LOCUS       DAOPOA010000199         3138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_33681_length_3138_cov_3.217970, whole genome shotgun sequence.
ACCESSION   DAOPOA010000199 DAOPOA010000000
VERSION     DAOPOA010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33681_length_3138_cov_3.217970"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PKJ17_05250"
     CDS             complement(<1..1108)
                     /locus_tag="PKJ17_05250"
                     /inference="COORDINATES: protein motif:HMM:NF017321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOD75411.1"
                     /translation="MELFSDDTSFTGRSQKPLADRVRPRSLDDVVGQEHLLGQGKLLR
                     VLIERGDVPSFIFWGPSGVGKTTIGWLIGRIMKLPYVFLSAVSIGVKEVKEVIQKARN
                     QRIILFVDEFHRFNKLQQDTFLPHVENGTIVLIGATTENPSFEIISPLLSRMRVLTLK
                     PLVTTDLVKILTRALNDDEELKKGNISIDARTIEEIAYLADGDARRALNLLEVCWKMV
                     REGSSRNPVIDHDIVQEAYQKKTARYDKTGEMHYDLISALHKSMRGSDADASLYWLAR
                     MIEGGEDPLYIMRRLVRFASEDVGNADPHALTLTISATEAFKFIGPPEGYLALAQAAV
                     YLSLCEKSNAVYTAYGQAARDARDLPEYPVPMHIR"
     gene            complement(1182..1400)
                     /locus_tag="PKJ17_05255"
     CDS             complement(1182..1400)
                     /locus_tag="PKJ17_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HOD75412.1"
                     /translation="MIIGEIQKMGTDRIIVTVKEFKGKTYIDVRNFFENDEGEMVPTK
                     KGVSLTPENLDELIRLLGEAKKQLPTEK"
     gene            1708..2958
                     /locus_tag="PKJ17_05260"
     CDS             1708..2958
                     /locus_tag="PKJ17_05260"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD75413.1"
                     /translation="MMNGAGASKSQGLHWAWIILAVCFVNLFINYGIRLGYGVILPEM
                     IRTMGITRKEGGEIFNAYFYAYICFSPFVGYITDRLGARVVIPVFGVVLGVGTFLMGL
                     SNGFWQAAFTFCFVGIGAAAMWTPIITLVQRWFAIHKRGMALGILSAGFGLGFANMGR
                     LYPVIVAHWSWRYCWYILGIAALIMIVINVILLRSKPEDRGLAPWGTRPEEPAPAAPA
                     APAGKQRSCYGEILSAKRFWMIAASYFAIGATLYMITTFMVDYARYQLGFAYDRASLL
                     ATMHGFGQVVGVLTIPAISDRFGRKGTILTSNLLIALIIIWIILSGSNATMLYTSIFI
                     FGIFYGATFPLYGACGGDYFRKEIIGTVIGAFTPFYAFGAIAAHWFGGYVRDVTGSFS
                     IPFMVAIGLAALSAVLMGFVKKEE"
BASE COUNT          579 a          893 c          936 g          730 t
ORIGIN      
        1 tgcggatatg catggggacg gggtattccg ggaggtcccg ggcgtcccgt gccgcctgac
       61 catacgcggt gtagacggcg ttgctctttt cgcagagaga caggtacacg gccgcctgcg
      121 cgagggccag ataaccctcg ggggggccga tgaacttgaa tgcctccgtg gcgctgatgg
      181 tgagcgtgag agcgtgaggg tcagcattgc cgacatcctc cgaggcgaag cgcacgaggc
      241 ggcgcatgat gtaaaggggg tcctcgcctc cttctatcat ccgggcaagc cagtacaggg
      301 acgcgtcggc gtcggagccg cgcatgctct tgtggagcgc gctgatgagg tcgtagtgca
      361 tctcgcccgt cttgtcgtac cgggcggtct tcttctggta ggcttcctga actatgtcgt
      421 gatcgatgac ggggtttcgc gaagagcctt ccctgaccat tttccagcac acctccagga
      481 gattcagagc gcgtcgcgcg tcgccgtcgg cgaggtacgc gatctcctct atcgtccgcg
      541 cgtcgatgga gatgttcccc tttttgagtt cttcgtcatc gttcagggcc ctcgtcagga
      601 tcttcacgag gtcggtggtg acgaggggtt tcagggtgag gaccctcatg cgggacagga
      661 ggggagagat gatctcgaag gaggggtttt ccgttgtggc gccgatgagg acgatggtgc
      721 cgttctccac atggggaagg aacgtgtcct gctggagctt gttgaagcgg tggaattcat
      781 cgacgaagag gatgatcctc tggttccggg ccttttgaat gacctccttg acctccttga
      841 cgcctatgga gacggcggaa agaaagacgt aggggagctt cattatcctg ccgatgagcc
      901 agccgatggt ggtcttgccg acgccgctcg gcccccagaa gatgaaggag gggacatcac
      961 ctctctcgat gaggaccctc aagagctttc cctgtccgag taggtgctcc tggccgacga
     1021 catcgtcgag cgacctgggg cgcaccctgt cggcgagagg tttttgggac ctacctgtga
     1081 aagacgtgtc gtccgaaaaa agctccacta acctgtcctt ttgtcccggg gtcccgaaac
     1141 gggccgggac tcggttgtgt tcgagatgta ccgccggggg atcacttctc tgtcggaagt
     1201 tgtttcttcg cttcacccag gagccgtata agctcgtcga ggttctccgg cgtcagcgac
     1261 acccccttct tcgtgggcac catctccccc tcgtcgttct cgaagaagtt cctaacgtcg
     1321 atgtaggtct ttcccttgaa ctccttcacc gtcacgatga tcctgtccgt acccatcttt
     1381 tgtatctcgc cgattatcat ggcgccctcc attcagcttt tattcactat agcacgttcg
     1441 gacactggag ggaagaaggc ttgaaccgct tgagccgctc gaacagcttg agcgggaaag
     1501 gcaaaagaca acagagacaa cagctcggcc cggagcccgg gtgataaccg ggagtcgggc
     1561 cgtactctca tgtcctttct tcgtttctca acgttcaagc ggctcgagag gctcaagcgg
     1621 tcttttatga ccgtcttttc tctccttgca ttccatcctt ccttgtccta gaatgtctct
     1681 acaccagaaa aacacgcagg gggagttatg atgaatggag ccggagcttc gaagtcgcag
     1741 ggtcttcact gggcgtggat cattctcgcg gtatgcttcg tcaacctctt catcaattac
     1801 ggcattcgcc tcgggtacgg tgtcattctg ccggagatga tacggaccat gggaataacc
     1861 cggaaggaag gtggggagat cttcaacgcc tacttctatg cctacatctg tttttcgccc
     1921 ttcgtgggat atatcacgga ccgtctgggt gcccgtgtcg tgatacccgt cttcggcgtg
     1981 gtcctggggg tgggaacatt cctgatgggg ctctccaacg gtttctggca ggcggctttc
     2041 accttctgct ttgtcggtat cggggcggcg gccatgtgga cgcccatcat caccctcgtg
     2101 cagagatggt ttgccatcca caaaaggggt atggcgctcg ggatactgtc ggccgggttc
     2161 gggctcggtt tcgctaacat gggacggctg tatccggtca tagtggccca ctggagctgg
     2221 aggtattgct ggtacattct cggtatcgca gccctcatca tgatcgtgat caacgtgatc
     2281 ctcctgagaa gcaaacccga ggataggggc ctggctccgt gggggacgag gcctgaagaa
     2341 cccgcgccgg ccgcgccggc ggcacccgcc ggcaaacagc gttcatgcta cggggagatc
     2401 ctctccgcga aacgcttctg gatgatcgcg gcctcctatt tcgcgatagg cgcgacactc
     2461 tacatgataa ccactttcat ggtcgactat gcgcggtacc aactgggctt tgcctacgac
     2521 cgggcatctc ttcttgcgac gatgcacggg ttcggccagg tggtgggggt cctcaccata
     2581 ccggcgatct ccgatcgttt tggccgaaag gggacgatac tcacgtcgaa ccttctcatc
     2641 gcgctgatca tcatctggat aatcctttcc ggttccaacg cgacgatgct ttacacgagc
     2701 atattcatct tcggcatatt ctacggcgcg acctttcccc tgtacggcgc ctgcggcggc
     2761 gactatttca gaaaggagat catcgggacg gtgatcggcg ccttcacacc tttttatgcc
     2821 ttcggagcca ttgccgccca ctggttcggc ggctacgtcc gcgacgtgac aggctccttc
     2881 tccatcccct tcatggtcgc cataggcctc gccgcactat cagcggtgct gatggggttc
     2941 gtgaagaagg aagagtagag gtcttacctc tcgcccgagc tgtgagccgg cgaagagctt
     3001 gaagaccttg cctctcgccc gcttcgctca agcccgcaga gatcgcagag aaaagattcg
     3061 gccggatttc gaagccttcc aaaatccggc cgtctttgca tcccgtctgc gacgggaggc
     3121 aaaagaaaag gattcttt
//
LOCUS       DAOPOA010000200         3128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_33813_length_3128_cov_6.425317, whole genome shotgun sequence.
ACCESSION   DAOPOA010000200 DAOPOA010000000
VERSION     DAOPOA010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33813_length_3128_cov_6.425317"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKJ17_05265"
     CDS             <1..665
                     /locus_tag="PKJ17_05265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOD75414.1"
                     /translation="EGRKIYDNIRKFIRYLLTTNTGEIMTIFFAQILGFPIPLLPIHI
                     LWINLVTDSLPALALSVEPAEDDVMQRPPRDPRESIFSGGLGIHTIWVGALMAFLVLS
                     VQYFSMASFNGEWQTMVFTVLCFTQVAHVLAIRSDRKSLFKQGLMSNKALLGAIVLSI
                     LMQLAVIYVGPLNAVFRTQPLTLGELAACVGISSIVFFAVETEKLIYRVRARRAAGPV
                     AS"
     gene            complement(689..1867)
                     /locus_tag="PKJ17_05270"
     CDS             complement(689..1867)
                     /locus_tag="PKJ17_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HOD75415.1"
                     /translation="MGPRSKREYTEAVFLRYKKASRKGKTAILDEFCATLGCHRKHAI
                     RVLKGFRRFTAPKQKKRGRRPVYDADVIGVPLRRIWLAANLPCSKRLRAILPLWLPGY
                     AERFGPLPLETVKALKRISSATIDRLLKPVRARYTQHGRSTTKPGTLLRRHIPVLTSQ
                     WNESRPGYLEADTVAHCGQSMSGMFAFTIDLVDIATGWTEQRAVWGKGETSIVEQIMD
                     VERSLPFDLLGFDCDNGSEFLNHHLYRYLTGRRSPVAFTRSRAYHKDDNAHVEQKNWT
                     HVRQWLGYERLDHPGVVELLNELYRTEWRLFHNFFCPSVKLVSKERIGSKTIRRHDSP
                     KTPYQRILESPYIELRIKKDLSRQFETLNPFTLRKAMEKRLHKISQLCYNSPSHKVTI
                     "
     gene            2034..3017
                     /locus_tag="PKJ17_05275"
     CDS             2034..3017
                     /locus_tag="PKJ17_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75416.1"
                     /translation="MEGSSANNGYVAYFDVLGFTSRFLSGDLRNRYEKLVAAIDERGD
                     KDVTYFLFSDSIVVISKIFAKVRETARSVYTWGILNDFWLRGGIAHGGLTPLDFSSFV
                     SGNRVILPFLGEGYLKAYKLESTLNISGIALDGIFFEPGKECAALKRRVDFVEYEEYL
                     PKRGYEDTKLLLLPEEGSLRQIVDTMYFEEMLKSHINDIDKYINTFIFFIETLLRTAD
                     HPTVVKFLDNLLKQLELHGSHVLIPSKVIIIFVAVIEGLIRRFRSGAFDDIPGNPRER
                     LETYVGDILGALKTQGYLPTFVDYVLEYDKTRHTTVYKDINRLRADWSDFH"
BASE COUNT          644 a          926 c          858 g          700 t
ORIGIN      
        1 gggaaggaag gaagatatac gacaatatcc gcaagttcat acgatacctc ctcacaacga
       61 acaccgggga gatcatgacg atattcttcg cccagatcct cggtttcccc attcccctct
      121 tgccgataca catcctctgg atcaacctag tcacggacag tctccccgct cttgcccttt
      181 cggtggaacc ggcggaggac gacgtcatgc agcgcccgcc gagagacccc cgcgaaagta
      241 tcttctcagg cgggctcggc atccacacga tttgggtcgg ggccctcatg gcctttctcg
      301 tcctctccgt gcaatacttc tccatggcat cctttaatgg ggaatggcag acaatggtct
      361 tcacggtact ctgtttcacc caggttgcac acgttctggc gatacgctct gacaggaagt
      421 ccctcttcaa gcaaggcctc atgtccaata aggcccttct cggcgcgatc gtcctgtcca
      481 tactgatgca gctcgccgtc atctacgtgg gtcccctcaa cgcggtcttc aggacccagc
      541 ccttgaccct cggggagctg gcagcctgcg tggggatctc atcgatcgtc ttctttgccg
      601 tcgagacaga gaagctcatc tatcgcgtga gggcgcgacg cgcggcgggc ccagttgcct
      661 cataattaaa tgttaccgag gaaggtcatc agatcgtgac cttatgactg ggactgttat
      721 agcagagctg cgagatttta tgaagccttt tctccatggc tttcctcagg gtaaaagggt
      781 tcagggtctc gaactgtcgc gagaggtctt tcttaatgcg gagctctatg tacggtgact
      841 ccaggatccg ctggtacggg gtcttcggtg aatcatggcg ccgtatcgtc ttcgatccga
      901 tcctttcctt cgagacaagc tttacggaag ggcagaagaa gttatggaac agtctccatt
      961 ccgtgcggta cagctcgttg agcagctcaa cgacgccggg gtgatcgagc ctctcgtatc
     1021 cgagccattg cctcacgtgg gtccagttct tctgctcgac atgagcgttg tcgtccttgt
     1081 ggtaggcccg gctgcgggtg aaagcgacag gactcctcct tccggtcagg tacctgtaga
     1141 ggtgatggtt gaggaactcg gacccgttgt cgcagtcgaa accgagaaga tcgaagggca
     1201 gggatcgttc gacatccatg atctgctcca cgatggaggt ctcccccttc ccccatacgg
     1261 cccgctgttc cgtccagccc gtggcgatat cgacgaggtc tatggtgaag gcaaacatgc
     1321 ccgacatcga ctgcccgcag tgggccaccg tgtccgcctc gagatagccc ggtctcgact
     1381 cgttccactg gctggtgagg acggggatgt gccgcctcag gagggtgccc ggttttgtcg
     1441 ttgacctccc gtgctgcgtg taccgcgcgc ggacgggctt caggagcctg tcgatggtgg
     1501 cggaggatat cctcttcaag gccttgacgg tctccagggg cagaggaccg aacctctcgg
     1561 catatcccgg aagccacaga ggcaggatcg ccctgaggcg ctttgaacag ggaagattcg
     1621 cggcgagcca tatcctcctg aggggtacgc ctatgacatc ggcgtcgtat acgggacggc
     1681 gcccccgctt cttctgtttg ggtgcggtga agcgcctgaa ccccttgaga acccgtatgg
     1741 catgcttgcg gtggcagccg agggtggcgc agaactcatc cagtatggcc gtcttccctt
     1801 tgcgggaggc cttcttgtac cgtaagaaga cggcttccgt atattccctc ttcgatcgcg
     1861 gacccataca caacccctcc tttcggggct attatttcgg taacatcctt tatgagtcaa
     1921 cgatctttct ctcaccaccc cttgggtaac attttttatg aggcaattcg gggcaacgca
     1981 aagggttgat ttttcataat gatgcccctt ataatgtgaa ccaggtcatg tcaatggaag
     2041 gatcctcggc gaacaacggg tatgtcgcgt atttcgacgt gctgggattc acgtcccggt
     2101 tcctgtcggg cgatctcagg aaccgctacg agaagctcgt tgccgccatc gacgaacggg
     2161 gggacaagga cgtaacctat ttccttttct cagacagcat cgtcgtcatc tcgaagatct
     2221 ttgcaaaggt gcgggagacg gcacgctccg tctacacctg gggcatcctg aacgatttct
     2281 ggctccgcgg cggcatcgcc catggcggtc tgacgccgct cgacttctcc agtttcgtga
     2341 gcggcaacag ggtcatcctt cccttcctcg gcgagggcta cctcaaggcc tacaagctcg
     2401 aatcgacgct gaatatctcg ggcatcgccc tcgacggcat cttcttcgaa cccggaaagg
     2461 aatgcgctgc cctgaagaga cgggtcgact tcgtcgagta cgaggaatac ctgcccaagc
     2521 gcggatacga ggacacgaag ctccttctgc ttcccgagga ggggtccttg aggcagatag
     2581 tggacacgat gtatttcgag gagatgctca agtcgcacat caacgacatc gacaagtaca
     2641 tcaacacctt catcttcttc atcgaaacgc tcctgaggac ggcggaccac ccgacggtcg
     2701 tcaagttcct ggacaacctg ctgaagcagc tggaactcca cggcagccac gtgctcatcc
     2761 ccagcaaggt catcatcatc ttcgtggccg ttatcgaagg cctcatcagg cgtttcagat
     2821 caggcgcctt cgacgacatt cccggaaacc cccgggaaag gctcgaaacc tacgtcgggg
     2881 acatcctcgg tgccctcaag acccaggggt acctccccac gttcgtggac tacgtcctgg
     2941 aatacgacaa gacacgccac acaacggtct acaaggacat caacaggctc cgcgccgact
     3001 ggtcagactt ccactagaaa aaaccgtttc agccgcttga gctgtttgaa cagcttgaac
     3061 gggaaaaagt gactgacccc tgatgtcaat aatggacatc aacgatctca tgcggcggtt
     3121 ctttccct
//
LOCUS       DAOPOA010000201         3126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_33851_length_3126_cov_3.881797, whole genome shotgun sequence.
ACCESSION   DAOPOA010000201 DAOPOA010000000
VERSION     DAOPOA010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33851_length_3126_cov_3.881797"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKJ17_05280"
     CDS             complement(<1..395)
                     /locus_tag="PKJ17_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOD75417.1"
                     /translation="MFNSITDIPGIKVGHATDLTGRTGCTAILCEEGAVAGIDVRGSA
                     SGTRQVDALNVGHIVERVHAILLCGGSSFGLDAASGVSRYLEEKGVGFDVGITRIPIV
                     PTAVIFDLLFGDHRIRPTAQMGYEACLNAG"
     gene            complement(426..926)
                     /gene="purE"
                     /locus_tag="PKJ17_05285"
     CDS             complement(426..926)
                     /gene="purE"
                     /locus_tag="PKJ17_05285"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOD75418.1"
                     /translation="MEKPKVLVLLGSDSDLGIIEDGLAFLKKAEVPYAIDISSAHRDP
                     EKTVNYARSAREEGIEVIIAVAGMAAHLPGVIASHTTLPVIGVPASGGILKGKDALYS
                     IVQMPKGIPVASVGIDAGTNAAILACEILSIKYDSVAKALIELKKDLKRENEQKSLNI
                     RNKLGN"
     gene            1088..1750
                     /locus_tag="PKJ17_05290"
     CDS             1088..1750
                     /locus_tag="PKJ17_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOD75419.1"
                     /translation="MAAVPLFKGLSEKQIDDLARAFPRRSYAAGETIFREGEPAEGFY
                     ILLSGRLKIYKLSMEGKEQILHIIEAGEPFAEVALFSETTFPAYAEAIRESEVMFFSR
                     KAFQQLVRNDPSIIMNMLAILSQRLKYFTRLVEDLSLKEVPQRLAAYLLFLADREGSR
                     QVSLGISKGQLASLLGTIPETLSRITARMAGGGLIEMQGRRITIIDRDGLEALAEGMR
                     GL"
     gene            2211..>3126
                     /locus_tag="PKJ17_05295"
     CDS             2211..>3126
                     /locus_tag="PKJ17_05295"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD75420.1"
                     /translation="MRSDPENSPGPFDFFVQMHLTERCNLACTHCYQEGRPGKEMTLE
                     EISRSLADISDTIRNWSETYEIPFAPSFNVTGGEPFLRNDLPDILRGISRCGFTCYLL
                     TNGTLVDRRRARMLAELPVGGVQVSVEGPEGIHEAIRGKGSFTSAMRGIGHLLDAGVT
                     VTMNVTLSELNGDLFPDIVALAAATGVQRLGFSRLVPYGRGSALTGKMLGRERLRKLY
                     EEILSLAVPGLEIATGDPLATQLGDGAAGMDPEAPALGGCAAGVSGITLLPDGTLTPC
                     RRIGIPIGNILKEGLREVWANSEVLAALR"
BASE COUNT          660 a          887 c          940 g          639 t
ORIGIN      
        1 ccggcgttga ggcaggcctc gtagcccatc tgggctgttg gacggatcct gtgatcgccg
       61 aagaggaggt cgaagatgac agccgtgggg acgatgggaa tgcgggttat ccccacatcg
      121 aagccgacgc ctttctcctc caggtagcgc gacacgcccg aggccgcgtc gaggccaaaa
      181 gagcttccgc cgcacaggag gatcgcgtgg accctctcga cgatgtggcc cacgttcagc
      241 gcgtccacct gccgtgtccc tgaagcgctc ccgcgaacat cgatcccggc aacggccccc
      301 tcttcacaga gtatggcggt acaccccgtc cggcccgtga ggtcggtggc gtgtcccacc
      361 ttgatgcccg gaatatcagt tatggaattg aacatagacg atcacctttc aatgacgaga
      421 ttgtgtcaat tacccaattt gttccgaata ttcagggatt tctgctcgtt ttcccgcttg
      481 aggtccttct tcagctctat cagtgccttc gcaacggagt cgtatttgat ggagagtatc
      541 tcgcaggcca gtatggccgc gtttgtgccg gcatcgatcc cgacggaggc caccggaata
      601 cccttgggca tctgcacgat ggagtagagc gcatcctttc ccttgaggat gccgccgctg
      661 gccggaacgc ctatgacggg cagtgtcgtg tgggacgcaa tgacgcccgg aaggtgggca
      721 gccatcccgg caacagcgat gatcacctcg atgccttcct ctcgggcgct cctggcgtaa
      781 ttcaccgttt tttcggggtc ccggtgcgcc gacgaaatat cgatcgcgta gggaacctcc
      841 gccttcttga ggaaggcgag cccgtcttcg atgatgccga ggtcgctgtc gctgcctagc
      901 agcactaaaa cctttggttt ctccataccc gcccctccag ccccggttgg tttggtcaac
      961 acagtctgta actaatacca cattttagca cgtgaataca tccgttttgt agcctctggc
     1021 gcggcggtgt tgtcttttca ccgcaaatat gcgaatcaat atagcataaa tgttaagaat
     1081 ggacgccatg gcagcggtgc cgcttttcaa gggcctttcg gagaagcaga tcgacgatct
     1141 ggcgcgcgcc tttccgcgcc gctcctacgc ggcgggggag accattttcc gggaaggaga
     1201 gcccgctgaa ggcttctaca tactcctttc gggaaggctg aagatctaca agctctcgat
     1261 ggaaggaaag gagcagatac tgcacatcat cgaagccggc gagcccttcg cggaggtggc
     1321 tctcttttcg gagacaacct ttcccgccta tgccgaagcg atcagggaga gcgaggtgat
     1381 gttcttttcc cgaaaggcct tccagcagct tgtcaggaac gatccctcca tcataatgaa
     1441 catgctcgcc atcctgtccc agaggctcaa gtacttcacc cgcctcgtgg aagacctgtc
     1501 cctcaaggag gttccccaga ggctcgccgc gtatctcctg ttcctcgctg acagggaagg
     1561 ctcgcggcag gtctcgctcg gcatatcgaa gggccaactg gcaagcctcc tcgggaccat
     1621 accggagacc ctctcgcgca tcacggccag gatggccgga gggggactca tagaaatgca
     1681 ggggcgccgc atcaccataa tcgaccgcga cggcctcgag gctctcgccg aggggatgag
     1741 ggggctgtaa aaaggcggtt caaggtttca ggttccaggt cgcaggttaa agacaaaacg
     1801 ggaagcttga aagagtgccg gtgccggcag ctgacgacct gagacctgaa acgagaaacc
     1861 ttgagaccgt aacttcccat tatatcgctt agttacatgc aaggaaacgg gagtgtgggt
     1921 tgacctgtgt tgatagcctg ctataattaa ttccatgcat gtgattcgcg attcttatcc
     1981 gatgaggaaa gggaggtgag aggcacatga ggaaggacag tgaaagaaag accgtcgaaa
     2041 aggaggcctt gttctggtac ggcatcaaga aggcccttgt gaaggacacc acgaagagat
     2101 gccacaagtg aaagagctga aagggttgac gttgcaggtc tgcaacgtga acgcgaaagg
     2161 cagtgagggt caggatgctg tccccactgc ctttcttttg ggtgattgcc atgagatcag
     2221 atcctgagaa ctcgcccggc ccctttgatt tctttgtcca gatgcacctg acggagaggt
     2281 gcaacctggc gtgcacccac tgctaccagg agggcagacc cgggaaggag atgacactcg
     2341 aggagataag ccggagcctt gccgatattt ccgataccat ccggaactgg tcagaaacgt
     2401 atgaaatacc attcgccccg agcttcaacg tgaccggcgg cgaacccttc ctcagaaatg
     2461 accttcccga catcctgcgg gggatctccc gatgcggttt tacctgctat ctgctcacca
     2521 acgggaccct cgtcgacagg agaagggcgc ggatgcttgc cgagcttcct gtcggtggag
     2581 tgcaggtgag cgtggagggg cctgaaggga tacatgaggc gatccgtgga aaaggcagct
     2641 tcacctccgc catgcggggg atcggtcacc ttctcgacgc gggcgtaacg gtcacgatga
     2701 acgtgaccct ttcggaactg aacggggacc tctttcccga tatcgtggcc cttgccgccg
     2761 ccaccggtgt gcagcgcctc ggtttctcca gactcgtgcc ttacgggaga ggttcggccc
     2821 tgacgggaaa gatgctcgga agggaaaggc tcaggaagct ttacgaagag atactttcac
     2881 ttgccgtccc ggggctcgag atcgcaacgg gagatcccct ggcgacgcag ctcggtgacg
     2941 gcgctgccgg gatggacccc gaggcgcccg ccctgggggg atgcgccgcg ggagtatcgg
     3001 ggataacgtt gctccctgac gggacgctca cgccctgcag aaggatcggc atccccatcg
     3061 gtaacatcct gaaggagggt ctcagggagg tgtgggccaa ttctgaggtc cttgccgccc
     3121 ttcgtg
//
LOCUS       DAOPOA010000202         3126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_33852_length_3126_cov_3.749919, whole genome shotgun sequence.
ACCESSION   DAOPOA010000202 DAOPOA010000000
VERSION     DAOPOA010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33852_length_3126_cov_3.749919"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PKJ17_05300"
     CDS             <1..908
                     /locus_tag="PKJ17_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOD75421.1"
                     /translation="QFAGIYQGGWSREVVRGQVESSDCLLSLGAWITDLDTGLFSMNL
                     DHRRMVFAERGEVRIGNHYYHHIQLKDLIRTLTLAARPRSFLSSHPAEALHPRKPFVA
                     EPSQALTASRLYECLDDFLDDGMVLLSEPGDAFCAAPEFHIEEAENFIVQNYYSSIGY
                     CTPAALGVAMARPEKRPVVLTGDGAFQMTAQEISTLIRKRCPALIVLINNDGYLVERL
                     LHEDGPYNDIQMWQYSRLPGVLGDGSGAVGLRVATEGELAEAMSVAAREKDRFVLIEA
                     CLRKGDCSEGLSRLGESFRRAQVKK"
     gene            1680..3014
                     /locus_tag="PKJ17_05305"
     CDS             1680..3014
                     /locus_tag="PKJ17_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75422.1"
                     /translation="MSYTPSWKGCAHPRAFLFLLIFVLFLLFTIPARGQVVMTEKGKE
                     FEPKVLHVPYAFYNQSFGAAGGFVCGLTGVGQKQMSILGTVIAGTNDALALYIVTRDV
                     QVPFLTRLFIDSDLALSTFGKLQSYTDGNRDYTGERAGSNDSSYNNYIEGPGNDNFAR
                     VKFRYLLPLGNGRDEVVSSVVLDRGLLSQGSVGGDSWNPIVSGKTFLEMKPYWRSQSV
                     RSDDRRVDRQTNGVEFSVFRENTDFPRNPSKGSTLRARYTRDWGLFDSTNPYDVLDVE
                     FSKYFSLGPSKMFRQRVIAFDVWSANVFSWDDHNTRNSGQAVYHRPPPYAGAMLGGLW
                     RMRAYPTSRFNDQAAVYYALEYRVIPEWNPFEKIDWVRKHLDIAWWQWVPFVEAGRVA
                     PQWTVDELHTKMQWDVGLGIRAMAKGLTVRIDTAVSNENWGVQMMIGHPFQF"
BASE COUNT          619 a          938 c          950 g          619 t
ORIGIN      
        1 cccagtttgc gggcatctac caggggggat ggagccggga ggtcgtccgg gggcaggtgg
       61 agtcgtccga ctgtctcctt tccctcggcg cctggataac ggacctcgac accggccttt
      121 tcagcatgaa cctcgatcat cgccgcatgg tctttgctga gaggggtgag gtccgcatcg
      181 gcaaccatta ttatcaccac atacagttga aggacctcat ccgcacgttg acgcttgcgg
      241 cccgcccccg ctcgttcctg agctcacatc cggccgaagc cctccacccc aggaaaccct
      301 tcgtggcgga gccgtcgcag gccctgacgg cgtctcggct gtacgaatgc ctcgatgatt
      361 tcctcgacga cgggatggtc ctcctgtcgg agccgggcga cgccttctgt gcggcgccgg
      421 agttccacat cgaggaggca gagaacttca tcgtccagaa ctactattcc tccatcggct
      481 actgtacgcc tgccgccctg ggtgtcgcca tggcgcgtcc cgaaaaacgc cccgttgtcc
      541 tcaccggcga cggcgccttc cagatgacgg cacaggagat ctcgacgctc atccgaaagc
      601 gctgccccgc cctgatagtc ctcatcaaca atgacggcta tctcgtcgag cggctcctcc
      661 atgaggacgg cccttacaac gacatccaga tgtggcagta ttccaggctt cccggggtgc
      721 tcggcgacgg gtccggcgcg gtcggtctca gggtggccac ggagggggaa ttggccgagg
      781 ccatgagcgt cgccgcgagg gaaaaggaca ggttcgttct tatcgaagcc tgtctgcgga
      841 agggcgattg ctccgagggc ttaagccgcc tcggcgaatc attccgaaga gcgcaggtga
      901 aaaaatagcg ggagatggtc gcatatgaac gagatgccgc tcagagatat cagggtcgtg
      961 aacctggaac tccttccggc cttcggtcct tgccagggta cggcctggac tggacaacgc
     1021 tgcacagccg ctacccgagg ctttgccagg tttccatcgt cggataccct tcaccccatc
     1081 aggaaagatc gggccacgac ctgacgtacc aggcccagac cggtgttctc tgcccgccct
     1141 ctctcccgcg gacattcctc gctgaagacg ggaccgcaag acggttgaag ttctagagtc
     1201 gtttgacagg gctttcatgg aatccggtcc cggtccgttt tgtgttccgt catcccgccc
     1261 ttcacagcct cagtccatca ccccttcaca gcctcaatgc gtcatcccgg cgaaggccgg
     1321 gatcgaggaa aaccctgttg cagaggaaag cgcacaaaac gtacccgaag agagtgatat
     1381 gaagaagcgc cttcgggttt ttcactgttc agtggtcgtt tgctggcatt cctttctgga
     1441 tcccggcctt ccgccgggat gacgggatga cgggatgacg gaaaatgagg gatgaaatga
     1501 tggggaagtt atttccgaca agaggtcgtt ggaccttcca gcacccaaac atcgtcgtgt
     1561 cgctcccgtc gtttgtgctt gcaaatttgg tgaaattgca ggagaatgag gcggcgagac
     1621 cgcagggaaa gattccccgc ggaacaaccg tatcaaacca acaacgagga gtctgctcta
     1681 tgtcatatac gccgtcgtgg aaggggtgtg cgcacccccg cgcgtttctg ttcctcctca
     1741 tcttcgttct tttcctcctt ttcaccatcc cggcacgggg tcaggtcgtc atgacggaaa
     1801 agggcaagga attcgagccc aaggtgcttc acgttcctta tgccttctac aaccagtcct
     1861 ttggagcggc gggaggattc gtctgcgggt tgaccggggt gggccagaag cagatgagca
     1921 tcctgggcac agtgatcgcg ggcaccaacg acgcccttgc cctgtacatc gtgacgcggg
     1981 acgtccaggt gcctttcctc acgagactct tcatcgactc cgaccttgcg ctgagcacct
     2041 tcggcaagct ccagagctat acggacggga acagggacta caccggtgag cgggcgggca
     2101 gcaacgattc gtcctataac aactacatag aaggaccggg gaacgacaat ttcgcccggg
     2161 tgaagttcag gtacctgctt cccctgggca acggcaggga cgaggtggtg agttccgtcg
     2221 tcctcgacag gggcctactg tcgcagggga gcgtgggagg ggattcctgg aaccctattg
     2281 tgagcggcaa gacgtttctt gaaatgaagc cgtactggag gagccagtcc gtccgctccg
     2341 acgaccggcg tgtcgacagg cagacgaacg gcgtcgagtt ctcggtgttc cgggagaaca
     2401 cggattttcc caggaacccc tcgaagggca gcaccctgcg cgcccggtac acgagggact
     2461 ggggcctctt cgacagcacg aacccctacg acgtcctcga cgtggagttc agcaagtatt
     2521 tctccctggg gccgtcgaag atgttccggc aacgggtgat cgccttcgac gtctggtcgg
     2581 cgaacgtctt ctcctgggac gaccacaaca cgagaaacag cggccaggcc gtgtaccaca
     2641 ggcctccccc ctatgccggg gctatgctgg gcggcctgtg gcgcatgcgg gcgtacccga
     2701 catcgcgttt caacgaccag gctgcggttt actacgcttt ggagtacagg gtcatccccg
     2761 agtggaaccc cttcgagaag atcgactggg tgcgaaagca cctcgatatc gcgtggtggc
     2821 aatgggttcc cttcgtggag gcgggcaggg tggcgcccca gtggacggtg gatgagcttc
     2881 atacgaagat gcagtgggac gtgggcctcg gcatccgcgc catggccaag ggcctcacgg
     2941 tgcgcatcga cacggccgtc tcgaacgaga actggggcgt ccagatgatg atcggccacc
     3001 ccttccagtt ctagaggcgg tttaaaccgc tcggcagcct gttgacaaac ttcttttcca
     3061 tgtcatcccg gcgaaggccg ggatccagaa gggactggca gtacttgact agcgaacagt
     3121 gaaatc
//
LOCUS       DAOPOA010000203         3126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_33853_length_3126_cov_3.419407, whole genome shotgun sequence.
ACCESSION   DAOPOA010000203 DAOPOA010000000
VERSION     DAOPOA010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33853_length_3126_cov_3.419407"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /locus_tag="PKJ17_05310"
     CDS             <1..1828
                     /locus_tag="PKJ17_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOD75423.1"
                     /translation="VKKLEGTFCEIRGEIVSAHPGKKLIDGWRRYLAGKVDASGTRHA
                     PVVKALTIGDTASLDDRTKTLFLETGTSHILSISGSHFAVVAGFFFFIARLAFRASSR
                     MRQKGTDRAWAALLTIPFTILFMLVSGSSLPTIRATIMIIIYMLALFFERKGHSVNAL
                     FLSALIILVIFPHSLFSPSFQLTFVSVLFIILTSRAIHSLLAKTGRAVRWLLSLTAVG
                     LAATIGTLPVVLYHFHGFNPLGFLYNLAAVPLMCLVSTPLALAGLVAPFGAYLLRLSG
                     EVIEITIALLDTLNRGYIYPVIRPNLPETLIYFATLISLLFIRRRPVRFAFVAIVLPL
                     LIVTGFVTWQKRFHNNDLCVSYIDVGLGDAILVEAPRGVRLLIDGGGFHGTGFDTGRS
                     VIAPLLLGKKIATLDYVVNSHPHEDHIGGLRFILGHFGVRAYMGLTGPEAQPSMKRVT
                     DILRERCIPSVHLAAGDIIPLPSGSGIAVLTPSGTVSPGNLNDASLVLKMTLGDRDFL
                     FTGDIGEDVEKALILSRAPLRSSVLKVPHHGSRFSSTTHFIRAVKPDLAVLSVGPGIR
                     GIPSPETVARYTALSIPLYRTDRDGCITVCTDGKKLTVEKEN"
     gene            complement(1853..>3126)
                     /locus_tag="PKJ17_05315"
     CDS             complement(1853..>3126)
                     /locus_tag="PKJ17_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75424.1"
                     /translation="RSVRQDGTVRYDLNKKATLTADDGSVGGIVGVIIDISEQKRMEE
                     DILKAKNLQSLGTLAGGIAHDFNNLLMAIVGNLSLARMHTPDNGRLMDYLNEAERIAF
                     LGKALTQQLLTFSRGGNPIRSMVHPGILVRSVAERALRGFPIDCVCDISPDLFPIEAD
                     EDQIRQVIENILRNARESMPAGGKITITARNVSISPEDRLPLMTEDHVRISIADEGTG
                     IAPEDIPKIFDPYYTTKGMGSEKGVGLGLAISYAIVRKHNGSISIESTKGQGSVFHVN
                     LPAYKHEAAQEAAAARRPAGTREGRVLLLDDEELVLEIGKELLEYLGYTVTTVQGGEE
                     AVALYKQAMELKSPFDAVILDLAIPGSLGGKEVLRELLRIDPGVKAIISSGYLNDPVV
                     ADYREYGFQDVLTKPYDSSELDEKLKKVIEG"
BASE COUNT          572 a         1012 c          816 g          726 t
ORIGIN      
        1 ggtgaagaaa cttgaaggga ccttctgcga gatccgtgga gagatagtgt ccgcccaccc
       61 gggaaaaaaa ctgatagacg gctggagaag atatctcgcc gggaaggtcg atgcaagcgg
      121 tacccggcat gccccggtcg tgaaggccct gaccatcggc gatacggcaa gccttgacga
      181 caggacgaag accctcttcc ttgaaacggg aacatctcat atcctgtcca tatccgggtc
      241 acacttcgct gtcgtggccg gctttttctt ctttatcgca cggctcgcct tccgcgcatc
      301 ttcccgaatg cgtcaaaaag ggaccgatcg cgcatgggcc gccctcctga ccataccttt
      361 cacaatcctc ttcatgcttg tctccggttc aagccttccc accatacgcg cgacgatcat
      421 gatcatcatc tatatgcttg cgctcttttt cgaacgcaag gggcacagcg tgaacgcgtt
      481 gttcctcagc gccctcatca tactcgtcat attcccccac tcgctgttct ccccgtcctt
      541 ccagttgacg ttcgtcagcg ttctcttcat tattctcacg agccgcgcca tccattcctt
      601 gctcgcgaag accggtcgcg cggtccgatg gctcctgtct ctgacggccg tgggcctcgc
      661 ggccacgatc gggaccctgc ctgtcgtcct ctatcatttt cacggattca accccctggg
      721 ctttctctat aaccttgccg ccgtaccgct catgtgcctt gtctccacac cgctcgccct
      781 cgcgggcctc gtcgcgcctt tcggcgcgta cctcctgcgg ttgtcgggag aggtcatcga
      841 aatcaccatt gccctgctgg acacactgaa ccgcgggtat atctatcccg tgatacgtcc
      901 gaaccttccc gaaacgctca tctatttcgc caccctcatc tcgctcctct tcatccgccg
      961 cagaccggtg cgtttcgcct ttgtcgccat cgtcctcccg ctgcttatcg tcacgggttt
     1021 cgtcacgtgg cagaagcgtt tccacaacaa cgacctgtgc gtcagctaca tcgacgtggg
     1081 gttgggcgat gccatactcg tcgaggctcc ccgcggcgtc cgcctgctca tcgacggagg
     1141 cggtttccat ggcaccgggt tcgatacggg aaggtccgtc atcgctcctc tcctcctcgg
     1201 aaagaagata gcgaccctgg actacgtcgt gaacagtcat ccccacgagg accacatcgg
     1261 agggctgcgt ttcattctcg gtcatttcgg cgtgagggcc tacatgggcc tgacaggtcc
     1321 cgaagcgcag ccgtcgatga agcgcgtcac ggatattctc agagagcggt gcataccctc
     1381 cgtgcacctg gcagccggcg atatcattcc cctcccctca gggtcaggca ttgccgtgct
     1441 tacaccttcc ggcacggttt cgccgggcaa tcttaatgac gcgtccctcg tcctgaagat
     1501 gacactgggc gacagagact ttctcttcac cggggacatc ggggaagatg tcgagaaggc
     1561 actgatcctg tcgcgggcac cgcttaggtc ctctgtcctg aaggtcccgc accacggaag
     1621 ccgcttttcg agcacgaccc actttatccg cgctgtcaag cccgatctgg ccgtgttgag
     1681 cgtgggcccg ggcatccggg ggataccgtc tccggagacg gtcgcccggt acaccgccct
     1741 ctcgatccct ctttatcgga ccgatcgcga cggctgcatc actgtttgta ctgatggaaa
     1801 gaaattgact gtggagaaag agaactgaaa gtccttcccc gaaccccggt atctatcctt
     1861 cgatgacttt cttcagtttt tcgtcgagct cgctcgaatc gtagggtttt gtcagtacat
     1921 cctgaaaacc atactcccga tagtcggcga cgacggggtc gttgagatat ccgctcgata
     1981 tgatcgcctt gaccccgggg tcgatccgca ggagctcccg caggacctcc ttgcccccga
     2041 ggcttcccgg gatggcaagg tcgagaatga ccgcatcgaa gggacttttc agttccatcg
     2101 cctgtttgta gagagccacc gcttcctccc cgccttgcac ggtggtcacc gtgtagccaa
     2161 ggtactcgag aagttccttc ccgatctcga ggacgagttc ctcgtcgtca agaagaagga
     2221 ccctgccctc acgtgtgccg gccggcctgc gcgccgccgc tgcctcctgt gccgcctcat
     2281 gcttgtacgc ggggagattt acgtgaaaca ccgagccctg tccctttgtt gactcgatgg
     2341 aaatactgcc gttgtgtttc ctgactatcg cgtaggagat ggcaaggcca agcccgaccc
     2401 ccttttccga ccccatgccc ttcgtcgtgt agtaggggtc gaatatcttc ggaatgtcct
     2461 cgggtgctat gcctgtcccc tcgtcggcaa tggatatcct cacatggtcc tctgtcatca
     2521 gcggaagtct gtcctcgggg gaaatactca cattcctcgc ggttatggtg atcttaccgc
     2581 ctgccggcat ggactctctc gcgtttctga gaatgttctc gatgacctgc ctgatctggt
     2641 cctcatcagc ctcgatggga aagaggtcgg gagatatgtc gcagacgcaa tcgatgggaa
     2701 aaccgcgcaa tgcccgctcc gcgacagacc tcacaaggat gccggggtgc accatgctcc
     2761 ggatgggatt ccctccccgg gaaaacgtca aaagctgctg cgtcagggcc tttcccagaa
     2821 aagcgatcct ctccgcctcg ttaagatagt ccatcagccg tccattgtcg ggcgtgtgca
     2881 tcctggcgag cgacaggttg cccacgatgg ccatgagaag attgttgaaa tcatgggcga
     2941 tgccacccgc cagagtcccc agcgactgga ggttcttcgc cttcagaata tcctcttcca
     3001 tcctcttttg ttcgctgata tctatgatga cgccgacaat cccgcccaca cttccgtcat
     3061 cggccgtgag cgtcgccttc ttgttgaggt cataacgcac cgtcccatcc tgccgaacgg
     3121 atctca
//
LOCUS       DAOPOA010000204         3124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_33885_length_3124_cov_2.987292, whole genome shotgun sequence.
ACCESSION   DAOPOA010000204 DAOPOA010000000
VERSION     DAOPOA010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33885_length_3124_cov_2.987292"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3124
                     /locus_tag="PKJ17_05320"
     CDS             <1..>3124
                     /locus_tag="PKJ17_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOD75425.1"
                     /translation="IDVTADGYVTCTRTEKIESGTYHIELQKEGILSGLVKDSSGRPV
                     QGVEVRLNPGGRRQPREDGEEGEDGRVARPLITSTAGDGSYSFKKLKQGPSLVTFRKE
                     GYIAETLRVENVRSGETVNLPLVMYRPARISGKVLVEGLGIPAPDMDVAVQGAFNYST
                     VTYHDGTYSIGDIKPGTYYVRLNHRGFHALERSGVAIGEGDELRGNDFIVKPKDASIE
                     VYTSRYTFVPGQPVVFDLKTLRLEKVRVILYGVPIREMLHGQRSAAVDDPAKRGFKVV
                     RQWEESIKDFKPYEYRSQSIEVMKAVPLGSYCIEVTDGDKVRNRKFFSVTSIGVVVKS
                     SSDNILVYATDLIKNRPMPGVSITAFERTPSKPVSEHQLQSAEGIALMNSVARGKTDK
                     DGVYRAKVDSGRNLTVLATSNDAGYAFCDSGAPGGRASEKKRLFIYTDRPVYRAGDKV
                     HYKVVGKSSERTLVPMKGESIHYQIHNMDAEKIVDSGEFTLDEWGTYGGSVTLAAGMG
                     LGEYEIKAGPAPDSLFASGRFYVEQYRKPEFRIDIAPSKRFFVNRDTLEFKVDAKYFF
                     GSPLKGALVRYRFFEARLRDAGTRYWWEEDSPATPASTGRLKLEGEKYLDANGVAVLK
                     LSAGNFPYDREITLEATIVDGSNASVSSRSTVRVGRGEFYLKIDPVRQFFGEGEKKTI
                     KIVSLAHDGTPVSAAVNIKVYRYIWKASQMVYLHDSRPVMERQINTDKQGRGTLELPD
                     TLQYYGEYDIVAESKDQQDNMITASRVVWIYGRGSSRVDSRFKNLELAVSDPRMEKPG
                     EVTCLIKSRFADAHVLLTIEGKDIHESRVVKMHGNMIPVKLDIRSTYAPNVFIVGTLQ
                     KGRALYSSSVSVSLPAADTSLSIKMTPDKAKYLPGERVTMNISVANDAGRPVAADLSL
                     GVVDEAIYQIRPDHTPNIREFFYSKISNWVLTNYSYTFTVLAGAAKEGKVKIGEKFAD
                     TAFWKADIRTNERGQAFFSFNVPDNLTTWRLTARGHDLEGRAGERKGEILVTQDLIAR
                     IGKPRFFTRGD"
BASE COUNT          781 a          784 c          938 g          621 t
ORIGIN      
        1 gatagatgta acggccgatg ggtatgtcac gtgtacccgt actgaaaaga tcgaatcggg
       61 cacatatcat attgaattgc agaaggaagg gatcctgtcg gggctcgtca aagactcatc
      121 gggcagaccg gtacagggcg tcgaggtgag attgaacccc gggggacgcc gccaacccag
      181 agaagatggc gaggaaggcg aagacggcag agtggccaga cctctgatca catcaaccgc
      241 tggagacggt tcctattcct tcaaaaagct gaaacagggc cccagtctcg tgacgttcag
      301 aaaagagggc tacattgcgg aaaccctgcg ggtggagaac gtgagatcgg gagagacggt
      361 gaatctcccg ctggtcatgt atagacccgc gcgaatatcc ggaaaggttc tggtggaggg
      421 cctcggaatt ccggcacccg atatggatgt ggcggtgcag ggggcattca actattccac
      481 cgtgacatac catgacggga catacagtat cggtgacata aagcccggca cctattatgt
      541 gaggctcaac catcgcggtt ttcacgcact ggaaagaagc ggtgttgcga tcggggaagg
      601 agacgagctc aggggcaacg attttatagt gaagcccaag gacgcgtcaa ttgaggtcta
      661 cacgagccgt tataccttcg tgccggggca acccgtcgtt ttcgacctga agaccttgag
      721 gcttgaaaag gtcagggtaa tcctctacgg tgttcccatc agggaaatgt tacatggcca
      781 acggagcgcc gctgtggacg atccggcgaa gagaggtttc aaggttgtcc gtcagtggga
      841 agagtccata aaggatttca agccctacga gtatcgaagc cagtccatcg aggtcatgaa
      901 ggccgtacct ttgggcagct actgcataga ggttaccgac ggcgacaagg tccgtaacag
      961 aaagttcttc agcgtgacca gcatcggtgt cgtggtcaag agttcgtctg acaacatcct
     1021 tgtatatgcg accgacctga tcaagaaccg gcccatgccg ggtgtctcga taaccgcatt
     1081 tgaaagaacg ccgtcgaaac ctgtgtcgga gcatcaactt caatccgccg aaggtatagc
     1141 cctcatgaat tccgtcgcgc ggggcaaaac ggacaaagat ggcgtatacc gagccaaagt
     1201 ggattccggg cgcaatttga cggtcctcgc gaccagcaat gacgccggtt acgctttttg
     1261 cgacagtggc gcgccaggcg gccgggcaag cgagaagaag aggctgttca tatacacgga
     1321 ccgaccggtc tacagagccg gtgacaaggt tcattacaag gtggtcggaa aatcatccga
     1381 aagaaccctg gttccgatga agggcgagag tattcactat cagatccaca atatggatgc
     1441 cgagaagatc gtggattcag gcgagttcac cctggatgag tggggaacat atggcggttc
     1501 cgtcaccctg gctgccggga tgggtctcgg cgaatacgag atcaaagccg gcccagcccc
     1561 tgatagtctt ttcgcttccg ggaggttcta tgtagaacag taccggaagc ctgagttcag
     1621 gatcgacatc gcgccttcca agaggttctt tgtcaacagg gatacacttg aattcaaggt
     1681 ggatgcaaag tacttcttcg gctctcccct caagggggcg cttgtcaggt accggttctt
     1741 cgaggcgaga ctccgtgacg ccggcacgag gtactggtgg gaagaagatt ctccggcaac
     1801 tccggcatcc acgggccgtt tgaagcttga aggagagaag tatctcgatg cgaacggcgt
     1861 ggccgtcctg aagctgtcgg caggtaattt tccctacgac agggagatca cgctggaggc
     1921 gacgatagtc gacgggtcca atgccagcgt atcgtcgcgg tcgaccgtaa gggtcggcag
     1981 gggagagttc tacctgaaga tagatcctgt ccggcagttc ttcggggaag gcgagaagaa
     2041 gacgataaag atcgtatccc tggcgcatga cggcacaccg gtgtccgccg cggtgaacat
     2101 aaaggtgtac agatatatct ggaaggcctc ccaaatggtc tatctgcacg acagcaggcc
     2161 tgtgatggaa cgacagatca ataccgacaa acaaggccgg ggaacactgg aactgcccga
     2221 cacgctccag tattacggcg aatatgacat tgttgccgaa agcaaggacc aacaggacaa
     2281 catgatcacc gcctcccgtg tcgtctggat ctatggccgt ggttcctccc gggtggattc
     2341 gcgtttcaag aatctcgagc ttgccgtcag cgatccccgg atggagaagc cgggggaggt
     2401 cacctgcctg atcaagagca ggttcgccga tgcccacgtg ctccttacca tagagggcaa
     2461 ggacatacac gagagcaggg tggtgaaaat gcatggaaac atgatccccg tgaagctcga
     2521 catcaggagc acctatgcgc ccaatgtctt tatcgttgga acgctgcaaa aaggccgtgc
     2581 gctctattcg agcagtgttt ctgtgagcct gcccgctgcc gatacgtccc tttcgatcaa
     2641 gatgacgcct gataaggcaa aatatcttcc cggagagcgc gttaccatga atatatccgt
     2701 cgcgaacgac gcgggcagac ccgttgcagc agacctttcc ctgggcgttg ttgatgaggc
     2761 gatctatcag atacggcccg atcatactcc gaatatcagg gagttcttct attccaagat
     2821 atccaactgg gtccttacga actattccta cactttcacc gttctggcgg gtgcagcgaa
     2881 agagggaaag gtgaagatag gggagaagtt tgcggacacg gcgttctgga aggcggatat
     2941 aaggacgaac gagagagggc aggcattttt ctctttcaat gtgccggaca atctcacgac
     3001 ctggcgactg accgctcgcg gacacgacct cgagggcaga gcaggggaaa gaaagggtga
     3061 gatcctggta acgcaggatc tgatagcgag aataggcaag cccaggttct ttacccgggg
     3121 agac
//
LOCUS       DAOPOA010000205         3116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_33988_length_3116_cov_4.324077, whole genome shotgun sequence.
ACCESSION   DAOPOA010000205 DAOPOA010000000
VERSION     DAOPOA010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33988_length_3116_cov_4.324077"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1063)
                     /gene="trpS"
                     /locus_tag="PKJ17_05325"
     CDS             complement(80..1063)
                     /gene="trpS"
                     /locus_tag="PKJ17_05325"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOD75426.1"
                     /translation="MKRIFSGIQPTGEIHLGNYVGAIRNWVYLQDEYDCIFSVVDYHA
                     ITIEYPVEEMTKRTLDTAIILLACGLTPERSKIFVQSHVPEHTELAWIFNTVTPIGEV
                     ERMTQFKDKARQHRANINMGLMDYPVLQAADILLYKAGYVPVGEDQVQHIELCREVAR
                     KFNSRFTPIFPEPEELLSMAPRILGVDGQSKMSKSLGNYIGVLEEDDAVWEKLRTAVT
                     DVNRVRRKDPGNPEVCNIYTIHRAFSDNAMLIEIDKGCTSAGIGCIDCKKMLFENVKK
                     ELTPVREKARELENNMDYVVDVLKRGAAACREIADATMDEVHATMGLYPSR"
     gene            complement(1368..1856)
                     /locus_tag="PKJ17_05330"
     CDS             complement(1368..1856)
                     /locus_tag="PKJ17_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75427.1"
                     /translation="MAESILDGKKILAVDDEPDVLKVLEEEIMDACPKCAFDTATSFE
                     AASALLDSKSYDIVILDIMGVRGFDLLDMAVKKNLKVAMLTAHALSPEALKKSYEMKA
                     RAYLPKDKLGEIVPFLEDVLTTDFQSGWTRLFEKLHTFFTERFESDWEKKTGLTWREW
                     VK"
     gene            complement(1887..2621)
                     /locus_tag="PKJ17_05335"
     CDS             complement(1887..2621)
                     /locus_tag="PKJ17_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOD75428.1"
                     /translation="MTRKDPLVDLLIHDLTGPLSIVLTSVNNLLNKEDKCEPYNEYQR
                     DTLKRAQRNAEKAKSLLLEIIEVYRSEEGLFRKDRFLISDILREALVDAVEVAAPAVG
                     EELAHAEVCAYTEILARNGITIEVAGRYANTPFTHDPKKIRQILRNLISNALKYRKSR
                     MKLAITGDLDLVVAVEDDGEGIPPEKQDYIFKRFFHVNRKKDEPDGLGFGLSCVKNLV
                     EKMRGEISLKSGEGAGSCFTVRIPPL"
     gene            complement(2637..>3116)
                     /locus_tag="PKJ17_05340"
     CDS             complement(2637..>3116)
                     /locus_tag="PKJ17_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75429.1"
                     /translation="VVRDMMTGLLKNAVENTPDGGLVEVSVKEDERNVTVAVRDYGIG
                     ITEQNKEHVFEGFFHTQDTDLYGSKKAYDFGAGGKGLDLFQMKVYGRRFGFDISMTSS
                     RCDHIPTDRDLCPGRISECRFVSRREACLEAGGTTFYLTFPAAKEYLRTSTRDESDP"
BASE COUNT          600 a          870 c          883 g          763 t
ORIGIN      
        1 caggggtatt gcctcaggag cacagaggtg gtaagtatgt ccataccacg tgcctgagag
       61 gcgggcgccg gaaaaagcct taccgggaag ggtacaggcc catggtcgcg tggacctcgt
      121 ccatggtggc gtcggcgatc tccctgcagg cggcggcccc cctcttgagg acgtcgacaa
      181 cataatccat gttgttctcg agctcgcggg ctttctcgcg cacgggcgtc agttctttct
      241 tcacgttctc gaaaagcatt ttcttgcagt cgatgcaccc gatgcccgcc gacgtgcagc
      301 ccttgtcgat ctcgataagc atggcgttgt cggaaaaggc gcggtgaatg gtgtagatgt
      361 tgcacacctc ggggttgccg gggtccttcc tgcgcacgcg gttcacatcc gtcacggccg
      421 tgcggagctt ttcccagacc gcatcatcct cttcaagcac gccgatgtaa ttccccaggc
      481 tcttcgacat cttcgactgg ccgtcgacgc cgagtattct cggggccatg gagagcaact
      541 cctcgggttc gggaaatatg ggcgtaaagc gggagttgaa tttccgggcc acctcgcggc
      601 agagttctat gtgctgcacc tggtcttctc ccacgggaac gtaacctgcc ttgtagagca
      661 ggatgtcggc cgcctggagc acgggataat ccatgagccc catgttgatg ttcgcccggt
      721 gctgcctggc cttgtccttg aactgcgtca ttcgttcaac ctcgccaatg ggggtgacgg
      781 tattgaatat ccaggccagc tcggtatgct ccgggacatg ggactggacg aagatcttcg
      841 atctctccgg cgtgagcccg caggcaagga ggatgatggc cgtgtcaagg gtccgcttcg
      901 tcatctcctc cacggggtac tcgatcgtta tggcgtggta atcgacgaca ctgaagatgc
      961 agtcgtattc gtcctggagg tatacccagt tcctgatggc gccgacgtag ttgccgagat
     1021 gtatctcacc cgttggctgt atcccgctga agatccgctt catctatcct ccataaagaa
     1081 cggcaatccc tttctccgcg atttttatcg tgtttaatgt acatcaaacg gagggacaaa
     1141 tcaaatgact gtagaaaacg gttgaagccg cttgagccgt ttgaacagct tgaacgtaaa
     1201 gaggaagaga ccaaccgggc acggatgccg ggtgtcaccg gatttctttc aggctctcga
     1261 gcgacttgtg cgagtgcaca agcctttttg cctggaactt ttgaactctg gaacgagaag
     1321 aaaagcgggc cggaagcccg cttttcttct cttcgattga accttgttta cttcacccat
     1381 tccctccagg tgaggccagt ctttttttcc cagtccgatt cgaacctttc ggtgaagaag
     1441 gtgtggagct tctcgaaaag gcgggtccac cctgactgga aatcggtggt gaggacgtct
     1501 tcgaggaagg ggactatctc gccgagcttg tccttcggga ggtacgctct cgccttcatc
     1561 tcgtaggatt tcttgagggc ctcggggctc aaggcatgcg ccgtcagcat ggcgaccttg
     1621 aggttcttct tcacggccat gtcgaggagg tcgaagccgc gaacgcccat gatgtcgaga
     1681 attacaatat cgtagctctt cgagtcgaga agggccgatg ccgcttcgaa actcgttgcc
     1741 gtgtcgaagg cgcacttggg gcaggcgtcc atgatctcct cttccaggac cttgagaaca
     1801 tcaggttcgt cgtcgactgc cagtattttc tttccatcga gaatcgattc tgccattgtt
     1861 acctccttgt atttttagtt ccgacatcac agtggaggta tgcgcacggt gaagcatgag
     1921 cccgcgccct ctccgctttt cagtgatatc tcccccctca tcttctcgac aaggttcttt
     1981 acgcaggaaa gaccgaagcc caacccgtcg ggctcatcct tctttctgtt cacgtggaag
     2041 aagcgcttga atatgtagtc ctgtttctcc gggggtattc cctcgccgtc gtcctcaacg
     2101 gccacgacga ggtcgaggtc tcccgttatg gcaagcttca tcctgctttt ccggtacttg
     2161 agggcgttgg agatgaggtt cctgaggatc tggcgtatct ttttggggtc gtgggtgaag
     2221 ggggtgttcg cgtacctgcc ggcgacctcg atggtgatgc catttcgcgc gaggatctcg
     2281 gtgtaggcgc atacttccgc atgtgccagt tcctcaccga cggcgggggc cgcgacctcc
     2341 acggcatcaa cgagcgcctc cctcaggatg tcggagataa ggaatctgtc ctttctgaaa
     2401 aggccctctt cggagcggta gacctcgatg atctccagga ggagtgactt tgccttttcg
     2461 gcgttgcgtt gcgccctctt cagggtgtcc cgctgatatt cattgtacgg ttcgcactta
     2521 tcctctttgt tcagcaggtt gttgacactt gtcagcacga tcgacaaagg accggtgaga
     2581 tcatggataa gcaggtcaac gagggggtct ttacgggtca tttaccaggc cttggattaa
     2641 ggatctgact cgtccctagt actggttcta agatattctt tcgcggcggg aaaagtcaaa
     2701 taaaaagtag ttccgccggc ttccaggcag gcctcccggc gcgagacgaa acggcattcg
     2761 gagatccttc cgggacacag gtccctgtcg gtggggatgt gatcgcagcg ggaactcgtc
     2821 atcgagatgt cgaagccgaa acgccgccca tacaccttca tctggaaaag gtcgagtccc
     2881 ttaccgccgg ctccgaagtc ataagccttc ttcgaaccgt aaaggtcggt gtcctgggtg
     2941 tggaagaaac cctcgaagac gtgttccttg ttctgttcag tgatcccgat cccgtagtcc
     3001 ctgacggcga cggtgacgtt cctctcgtcc tccttgacgg acacctcgac gaggcccccg
     3061 tcaggcgtgt tctccacggc attcttgaga agccccgtca tcatgtcccg caccac
//
LOCUS       DAOPOA010000206         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_34073_length_3111_cov_2.760798, whole genome shotgun sequence.
ACCESSION   DAOPOA010000206 DAOPOA010000000
VERSION     DAOPOA010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34073_length_3111_cov_2.760798"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="PKJ17_05345"
     CDS             complement(<1..1635)
                     /locus_tag="PKJ17_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOD75430.1"
                     /translation="MRGFSLKTKMATVVSVLFLVIFAAGAFMFERVFEDHFKKTIADQ
                     QFELVSKIAYDVDLSIHEALKVLIDVSKLIPVADVNSYPAMQKHLERITETRFFLKGY
                     FDHGIVLYSRTGRVIGQVPHAPELSGKDFSDREYFQYTMKTKAPYISKPFRSIRPPYD
                     PIVMFSVPVFDKDGEISAVLGGAVSLLKDVTFGGIAGIKIGRTGYLYLFNTDRTIIVH
                     PDKTRILKQDLPRGANHLFDAAIDNWFEGSGETAPSKDVPGIATFKRFRNTDWILAAH
                     YPIKDAYAPIYRERWYIVAYTAIGIGLCVLIILIVMRRNLSPLSELASQAEGIGRTGD
                     PLGPVHVDTGGEIGALASSFNAMLERLAEREASLKETMEELRKLHNAVEHSSAVVVIT
                     DGKGNIEYVNPKFVEVTGYERSEVSGKTPRILKSGELPPEIYDDLWSTITSGEEWQGV
                     FHNRKKSGELYWAAASISPLKDATGTITHFVGVQEDITAVKLFEQELKGAKEAAETAN
                     QAKSEFLANMSHEIRTPMNAIIGMAELLLEGPLDEEQ"
     gene            complement(1635..2690)
                     /locus_tag="PKJ17_05350"
     CDS             complement(1635..2690)
                     /locus_tag="PKJ17_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOD75431.1"
                     /translation="MRSSRTVSLLLIVAATLLASSCGTSGAGPSLWGFALDGYPITPG
                     RIAGVEAATGLRPDMVVFFLQWPPVEDPSGGEFPLESLEAIAASGALPCITWEPMFLK
                     GDREVAISHTDVLGGRYDGFIRRFAEKARSWKKPVLMRFAHEMNLKRYHWGTAEDEYG
                     PESPAIYRRMYRHIVEIFRRSGAGNVLWVFCPNAESVPNGTYDPSAAWNVLSAYYPGD
                     DCVDVLGVDGYNWGTTRKKDVHGWESRWRSFQEIFEAAVADLRGLSSATSRKPLVVFE
                     TATVGTGGNKNEWIRDAMSAASTLGIDGIVWFQSDKEVDWRMETGSDGSHVPAVKGRR
                     SPGHDRLTELLEKGERP"
     gene            complement(2687..>3111)
                     /locus_tag="PKJ17_05355"
     CDS             complement(2687..>3111)
                     /locus_tag="PKJ17_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75432.1"
                     /translation="LLIFVPYIVITLTTFYWTLKQRSYTIRDLMSGQLLTFITFPVFM
                     KASVLALLGFKGTFGITPKGMSRALPLRAMWPQLLLWAICLCGAVWGVNRLVYEREPF
                     AGILVNVFWCLYHAAILSSIFYFNTPVEGAGEAGEEEG"
BASE COUNT          586 a          908 c          950 g          667 t
ORIGIN      
        1 ctgctcctcg tcgaggggcc cctccagaag cagctcggcc atgccgatga tggcgttcat
       61 cggcgtgcgt atctcatggc tcatgttggc gaggaactcg ctcttggcct ggttggcggt
      121 ctcggccgcc tctttggctc ccttgagctc ctgctcgaag agcttcacgg cggtgatgtc
      181 ctcctggacc ccgacgaaat gcgtaatggt gcctgtcgcg tccttgaggg gagagatgga
      241 tgccgcggcc cagtagagct ccccgctctt cttcctgttg tgaaagacac cctgccattc
      301 ctcaccggag gtgatggtcg accagagatc atcatagatc tcgggcggca gctctcccga
      361 tttgaggata cgcggcgtct tgcctgaaac ctcgcttctt tcataaccgg taacctcgac
      421 gaacttggga ttgacgtatt cgatgttgcc cttcccgtcg gtgatgacga caacggccga
      481 actgtgttcg acggcattgt ggagcttgcg gagctcttcc atggtctcct tcagagacgc
      541 ttccctttcg gcgagacgtt cgagcatggc attgaaggaa gaagccagcg cccctatctc
      601 cccaccggtg tccacgtgca cggggccaag gggatctccc gtccttccta tcccttcggc
      661 ctgcgaggcc agctccgaaa ggggcgacag gttcctgcgc atcacgatga ggatgatgag
      721 cacgcacagc ccgatgccga tggcagtgta ggccacgatg taccaccgct ccctgtagat
      781 gggcgcatag gcgtccttga tcggataatg tgccgccagt atccagtcgg tgttccgaaa
      841 gcgcttgaag gtcgctatgc ccggcacgtc ttttgagggc gccgtttccc cgcttccctc
      901 gaaccagttg tcgatggcgg cgtcgaaaag gtggttcgct ccccggggga ggtcctgttt
      961 aagtatccgt gtcttgtcgg gatggacgat gatcgtgcgg tccgtgttga agaggtagag
     1021 gtaacccgtc cggcctatct tgatgccggc tatgcccccg aaggtcacat ctttgagaag
     1081 ggacacggca ccgcccagta cggcggagat ttccccgtcc ttatcgaaga cggggacgga
     1141 gaacatgact atggggtcgt agggcggcct tatcgacctg aagggcttcg agatgtatgg
     1201 agccttggtc ttcatcgtgt actggaaata ctccctgtcg gagaagtctt ttccggacag
     1261 ttcgggggca tggggcacct ggccgatgac ccgtcctgtc cttgaataga ggacgatgcc
     1321 atggtcgaaa tatcccttca gaaagaaacg ggtctccgtt atcctctcca ggtgtttctg
     1381 catggcggga tagctgttga cgtccgcgac ggggattagc ttcgagacat cgatgagcac
     1441 cttcaaggcc tcgtggatgc tgaggtccac atcgtacgct atctttgaaa cgagttcgaa
     1501 ctgctggtcc gctatggtct tcttgaaatg gtcctcaaaa acacgctcga acatgaaggc
     1561 gcccgcggcg aagatgacga ggaagagtac ggagaccacc gtggccatct tcgttttgag
     1621 gctgaacccg cgcatcacgg ccgttctcct ttttcaagaa gctctgtgag gcggtcatgc
     1681 ccggggctgc gtcgcccctt cactgcgggg acgtggcttc catcggagcc ggtctccatc
     1741 cgccagtcga cctccttgtc ggactggaac cagacgatgc cgtcgatacc cagcgtgctt
     1801 gcggctgaca tggcgtcccg gatccattcg ttcttgttcc ctcccgtgcc gacggtggcg
     1861 gtctcgaaga cgacaagagg cttgcgcgag gtggcggagc ttaaacccct gagatccgcg
     1921 acggccgcct cgaatatctc ctggaaggac ctccagcggc tttcccatcc gtggacgtcc
     1981 ttcttcctgg tcgttcccca gttgtatccg tccacgccga ggacatcgac acaatcgtct
     2041 ccagggtagt aggcggacag gacattccat gctgcggagg ggtcataggt gccattcgga
     2101 acggactccg cgttcgggca gaagacccac aggacgttgc cggcgccgga ccgcctgaag
     2161 atctcgacga tatgccggta catcctccgg tagatggcag ggctttccgg gccgtattca
     2221 tcctccgccg tgccccagtg ataccgcttg aggttcattt cgtgggcgaa gcgcatgagc
     2281 acgggcttct tccaggagcg cgccttttcg gcgaaccgtc ttatgaaccc atcataccgg
     2341 ccgccgagca cgtcggtgtg ggagatggcg acctccctgt ctcccttcag gaacatgggc
     2401 tcccacgtga tgcagggcag ggcaccgctc gccgcgatgg cttcgaggct ttcgagaggg
     2461 aattcgccgc cggatgggtc ttcgaccggc ggccactgga gaaagaagac gaccatgtcc
     2521 gggcgaagtc cggtcgcggc ttcgacaccc gcgatcctcc cgggggttat ggggtacccg
     2581 tcaagggcaa aaccccacag cgaaggtccc gctccggagg tcccgcagga tgatgcgaga
     2641 agggtcgcgg cgacgatgag aagaagtgag acggtccggc tcgatctcac ccttcttcct
     2701 cccctgcttc cccggctcct tccacgggtg tgttgaagta gaatatcgac gagagtatcg
     2761 cggcgtgata aaggcaccag aaaacgttga cgagtattcc ggcgaagggt tccctttcgt
     2821 atacgagcct gttaacaccc cataccgccc cgcaaaggca tatggcccag aggagaagct
     2881 ggggccacat tgccctgagc ggcagggccc tgctcattcc tttcggcgtg atgccgaagg
     2941 tccccttgaa tcccagaagg gcgaggacgc tcgccttcat gaacacgggg aaggtgatga
     3001 aggtcagcaa ttgtcccgac atgaggtccc gtatcgtgta actgcgttgt ttcaacgtcc
     3061 agtagaaggt ggtaagggtg atgacgatgt aggggacgaa gatgaggagg t
//
LOCUS       DAOPOA010000207         3109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_34104_length_3109_cov_3.102161, whole genome shotgun sequence.
ACCESSION   DAOPOA010000207 DAOPOA010000000
VERSION     DAOPOA010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34104_length_3109_cov_3.102161"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKJ17_05360"
     CDS             complement(<1..182)
                     /locus_tag="PKJ17_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75433.1"
                     /translation="MQLEQTFMNFLLRHQERHNRTYHFLILMDAIISAAKRIEHYYIT
                     GALKGNLGCTDRVNVQG"
     gene            378..1607
                     /locus_tag="PKJ17_05365"
     CDS             378..1607
                     /locus_tag="PKJ17_05365"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOD75434.1"
                     /translation="MMKKGQAPFMLCCFVCIAVLLILTAGDVRAQRVLYEKPKMTKGP
                     GSLEVVLEPYLDKYDLPALGAAVVRDGKILAMGVVGTRAAGQQIPVTTGDRFHIGSDT
                     KAMTALLAAIYVDAGKLRWNSTVEEVFPELKKNLAAGWGQVSLEGLLSHTSGIPADDE
                     RFGKLLEGSLEQGDLDLRELRYWMLSRFLTQPVAAAPRKQFAYSNMGYMIAGAMIERA
                     VGRSWEELILEKVFVPLRLRSAGFGPQSTVGTVDAPLPHKIVDGKAKPMLAGPNADNP
                     LVLGPAGTVHMSLQDFARWAIWNLGKGKRGPGLVRSETLAKLTTPVIDIPPGKDAAPG
                     TPSGGRYALGWGEREYSWARGPFLTHAGSNGMNLAHIILDLKTDFGMVLMTNIGGKKA
                     DEALRGLAEELYRRYGK"
     gene            complement(1716..2609)
                     /locus_tag="PKJ17_05370"
     CDS             complement(1716..2609)
                     /locus_tag="PKJ17_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75435.1"
                     /translation="MKRSGIFCFCLLMLAAVIISETADAQPVKVSPVPKIARKKLTAD
                     LVVSNVRITPEHPRARKDMITIEVTVKNAGPGALPKVCSLAMDLGNMDTHPDYTNRII
                     PWYANNIPALAPGAEVKISKTITIPYPGTYKLGIIIITEGLQAGDENPVNNRHQMTFV
                     ASKPPDASDLVLDSVALNQEGRIVLRMHNSDDPIPDQDFHSSYVRVKVDNGLNKTIFL
                     RDMDPSGILKKGSTLPSGGAFPGGGTTHLIFAWPNSGPDGIKLDPGHAYIVEVTLDYN
                     GRIRDSNRSNNKRTVTLTYNP"
     gene            2922..>3109
                     /locus_tag="PKJ17_05375"
     CDS             2922..>3109
                     /locus_tag="PKJ17_05375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD75436.1"
                     /translation="MQETRFEKLHLSKMVQKALDTMGFSELSPIQEQSIPLVLEGMDI
                     IGQAQTGTGKTAAFGIPL"
BASE COUNT          645 a          809 c          936 g          719 t
ORIGIN      
        1 ccctggacgt tcaccctgtc ggtgcacccg aggtttccct tcagggcacc cgtgatatag
       61 taatgctcga tacgctttgc cgcgctgatg atggcgtcca taaggatgag gaagtgatag
      121 gtccgattgt gcctttcctg gtggcgaagc agaaaattca tgaatgtctg ttccagttgc
      181 atgcgtacct ctttttgccg taatttgccg gatgcggcag atacctgtga agtataagtg
      241 ctttcgggcg gtcaagtcaa aagataacga agcagaaaaa ccgtctttat ccttgaaccc
      301 ttggtcatag ttggctatta tcgttctgtc cggaagagtg gtcaccggaa ttcgtcccaa
      361 cgaaaggggg ttgttcgatg atgaaaaagg gtcaggcacc gtttatgctg tgttgcttcg
      421 tttgcatcgc cgttcttttg atcctgacgg ccggggacgt tcgtgcccag agggtcctgt
      481 atgagaagcc gaagatgacg aaaggccctg gctcactgga agtcgttctc gaaccgtacc
      541 tcgacaaata cgatctgccg gcgcttggcg ctgccgttgt cagggacggg aagatcctgg
      601 cgatgggggt ggtcggcaca agggcggcag gacaacagat cccggtgacg acgggggacc
      661 gttttcacat cggctccgat acgaaggcaa tgacggctct ccttgcggcg atatacgtgg
      721 acgcgggcaa gcttcgctgg aattcgacgg tggaggaggt attcccggag ttgaaaaaaa
      781 acctcgctgc cggctggggc caggtgtcgc tggagggact cctttcccat accagcggga
      841 tacctgccga tgatgaaaga ttcggcaagc tcctcgaagg gtcccttgaa cagggggatc
      901 tggacctcag ggaactgcgc tactggatgt tgtcgaggtt cctgacgcaa cctgtggcgg
      961 ccgcgccccg gaagcagttc gcgtattcca acatggggta catgatagcc ggggccatga
     1021 tagaacgtgc ggtcggcaga tcgtgggaag agcttatact cgagaaggta ttcgtcccct
     1081 tgaggcttcg cagcgccggt ttcggtcctc agtcgacggt gggaacggtg gacgccccgc
     1141 tcccgcacaa gatcgttgac ggcaaggcaa agcccatgct tgccgggccg aacgccgaca
     1201 accctctcgt tctgggaccg gccggcacag tgcacatgtc cctgcaggat tttgccaggt
     1261 gggccatctg gaacctggga aaaggcaaac gcggccccgg tcttgtccgt tccgaaacgc
     1321 tcgccaaatt gactaccccc gtaatcgaca ttcccccggg gaaggacgcc gcccccggga
     1381 cgccctcggg gggacgttac gcgcttggct ggggggaaag ggaatattcc tgggcaagag
     1441 gcccctttct cactcacgcc ggatcgaacg gcatgaacct tgcccatatc atccttgatc
     1501 taaagaccga tttcgggatg gtccttatga cgaacatcgg cggcaagaag gccgacgagg
     1561 ccttaagagg cctggccgag gaactctaca ggagatacgg gaagtaggca accgaaagac
     1621 cgttgcgggt tccgggttgc gcgttgcggg tcaaagacca gaggaagaag gagggtgcag
     1681 gtaccggccc tttccgcgaa acccgggagc tgttcttacg ggttataggt cagcgttacc
     1741 gttctcttgt tgtttgatct gttcgagtcc ctgatgcggc cgttgtaatc aagggtgacc
     1801 tccactatgt aggcgtgccc gggatcaagc ttgatcccgt cgggtcccga gtttggccag
     1861 gcgaagatga gatgagtcgt acctccaccc gggaatgctc cgcccgaagg aagggtgctg
     1921 cctttcttca agatacccga ggggtccatg tccctgagga atatcgtttt gttcagtccg
     1981 ttgtcgacct ttacccggac ataggagctg tggaaatcct gatcaggaat ggggtcatcg
     2041 ctgttgtgca tcctgagcac gatccgccct tcctggttca gcgccacgct gtcaaggaca
     2101 aggtccgaag cgtcaggggg tttcgaggcc acaaaggtca tttgatgccg gttgttgacg
     2161 ggattctcat cacccgcctg caagccttcg gtgatgatga taataccaag cttataggtg
     2221 cccggatagg gtatcgtgat ggtctttgat atcttcactt ccgcgccggg agccagggct
     2281 ggaatgttgt ttgcgtacca cggtattatg cggttggtgt agtcgggatg tgtgtccatg
     2341 ttcccgaggt ccatggcgag agaacagacc ttcgggagtg ctccgggacc ggcattcttt
     2401 acggtgacct caatggtgat catgtctttc ctggctctcg gatgctccgg ggttatcctg
     2461 acattggaca ccaccaggtc ggccgtcagt ttcttccgcg ctatcttcgg gaccggtgac
     2521 accttgaccg gctgcgcatc agcggtctcg gaaataatga cggcggcgag catcaagagg
     2581 cagaagcaga agattccgga tcgtttcata tcgtccccct ctgttcttgt tcttagtgtt
     2641 cttgttctca attctcaccg tcttttgtgt cgccgtcaag tcctttcagg agaggacagg
     2701 agccggggga ttgagaaggg cctgcgccct tgctttcaat ccctgtgctc tccgggcctt
     2761 cagttccttc ctgttgaaat tagaggtgtt tttttcaatg tttttgtggt agtatgctga
     2821 aagctactta aatcctctca gctttgccat gatccaacaa gaaaagaatg gtcgaatgcc
     2881 gggcgggcag aagcggcgca atattatttt acggagagta catgcaggaa acgagatttg
     2941 aaaagttaca tctgtccaaa atggtgcaaa aggccctgga tacgatgggg ttcagcgaac
     3001 tgagcccgat ccaggagcaa tcgatacccc ttgtcctcga aggcatggac atcatcggcc
     3061 aggcgcagac cggcacgggg aagacggccg ccttcggcat tcccctcat
//
LOCUS       DAOPOA010000208         3108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_34115_length_3108_cov_3.825090, whole genome shotgun sequence.
ACCESSION   DAOPOA010000208 DAOPOA010000000
VERSION     DAOPOA010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34115_length_3108_cov_3.825090"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKJ17_05380"
     CDS             complement(<1..231)
                     /locus_tag="PKJ17_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOD75437.1"
                     /translation="MFESLFGFISKDLAIDLGTANTLVYVKSRGIVSNEPSVVAVHKD
                     SRGTKKVIAVGEEAKKMLGKTPGNIIAIRPLKD"
     gene            335..1738
                     /locus_tag="PKJ17_05385"
     CDS             335..1738
                     /locus_tag="PKJ17_05385"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOD75438.1"
                     /translation="MLKFMRKYARSYLIKIIFGVIIIVFVFYFGAGSIRERETVIAEV
                     GGFDVREEEYRVSYNQEVEMFRQLYRDKMDDKLLAELKEKVLSDIINKYILLREAKKL
                     GISVSDQEFADLIGGVEAFRKDGKFDKETYLAVLKQNRTDAEQFEKSQKTMLLLRKVV
                     SIIRDTGAPVSDAAIWAGYVREKGKVDLAYSRFDPAAYRDRVSVTDKELNDLYEKEKD
                     RFRGENTFRLKYIVIDGAGNLKDDQAYMDLIKAKDIDAYAKQKGLAVSDTGDVRQSEL
                     FRRFKSLKIETWVRDLRKGDISLPARDDGKSYIFQLVTREDGKPMEKSAALASLKARI
                     TSEKAKEMARLAAEDALKAKSPGARTTTGFVSRTATSIPKLGEIPKDAMDLLGLTKEK
                     PVYGKPVEMNGDYYVFSFAGEQDPDRKEWEKNKEGFSRYYAARYREQFLRSFIEESKQ
                     AMLKDGKIKILKPAKEL"
     gene            1753..2748
                     /gene="tsaD"
                     /locus_tag="PKJ17_05390"
     CDS             1753..2748
                     /gene="tsaD"
                     /locus_tag="PKJ17_05390"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOD75439.1"
                     /translation="MLILGIDTSCDDTSVALMEEGRKIRSHIVSSQADLHSRFGGVVP
                     EIASRKHVEQIDVIYEEVLARAGASAEDVDLVGVTAGPGLIGSVLVGLCFAKGFCIAL
                     DRPLVGVNHIEAHAMSIFLEREVDFPFVALVVSGGHTIILLMEAPCRFRVLGSTRDDA
                     AGEAFDKIAKFLGIGYPGGRIIEEMARSGMRDYVAFPRPMMDDNTCDFSFSGLKTAFL
                     TYAKKHRIDDSTINHVLASFQEAICDVLVSKTVRAARLASVERIVVGGGVAANGRLRE
                     AFLERSRQEGLEVMISSPEFCTDNGAMVASLASFHAAAGMYSGLDIKGYSRMAIK"
     gene            2759..>3108
                     /locus_tag="PKJ17_05395"
     CDS             2759..>3108
                     /locus_tag="PKJ17_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOD75440.1"
                     /translation="MLKRRFSQNLIKDGNILRKMVSLAGITAKDTVVEIGAGQGDLTR
                     AIASAAARVIAVELDREMIPHLEAACLAFPNIEIVSGDILGVDIAGLARGRTVKAMGN
                     IPYGITGPIIFKLIG"
BASE COUNT          711 a          872 c          934 g          591 t
ORIGIN      
        1 gtccttgagc ggccggatgg cgatgatgtt tcccggggtc ttgccgagca tcttctttgc
       61 ctcttcgccc acggctatga cctttttcgt cccccgggaa tccttgtgaa cggccaccac
      121 ggagggctca ttggagacga tcccccggct tttcacgtag acgagcgtgt ttgccgtccc
      181 caggtctatt gccaggtctt ttgatataaa tccaaagagt gattcgaaca tcgactctcc
      241 cctcaaccgc gaattttaaa agttattgaa aaaacagcct taatatacta ttttaaagtt
      301 tcaaaatcaa taatctttag aggtaataga accaatgctc aagttcatga gaaaatatgc
      361 gagaagctac ctgatcaaga tcatcttcgg cgttatcatc attgtcttcg tcttctactt
      421 cggggcgggg tccataaggg agagggagac ggttatagcc gaggtcggtg gtttcgatgt
      481 cagggaggag gagtaccggg tgtcatacaa ccaggaggtt gagatgttcc ggcagctcta
      541 ccgcgacaag atggacgata agctcctggc cgaactgaag gaaaaggtct tgagcgacat
      601 catcaacaag tacatccttc tcagggaggc caaaaagctt ggcatctccg tgagcgacca
      661 ggaattcgcc gacctcatag ggggtgttga agcctttcgg aaggacggga agtttgacaa
      721 ggaaacgtat cttgccgtat tgaagcagaa caggaccgac gcggaacagt tcgagaagtc
      781 acagaagacc atgctccttt tgcgcaaggt ggtcagcatc atcagggaca cgggggcgcc
      841 ggtctccgac gccgccatct gggccgggta tgtgagggag aagggaaaag tggacctcgc
      901 gtactcgcgc ttcgaccccg cggcatacag ggacagggtc tcggtcacgg acaaggaact
      961 gaatgacctg tacgagaaag aaaaggaccg ctttcgcggt gagaacacct tccgcctgaa
     1021 atacatagtc atcgacggtg cgggcaacct taaggacgac caggcctaca tggacctcat
     1081 caaggcgaag gacatcgacg cctacgccaa acagaagggc cttgccgtgt cggacacggg
     1141 agacgtgcgc cagagcgaac tcttcaggag attcaagtcc ttgaagatcg agacgtgggt
     1201 cagggacctg aggaagggcg acatttccct tcccgcgcgc gacgatggga agtcctacat
     1261 tttccagctc gttacgcgcg aggatggaaa gcccatggag aagagcgcgg ccctggccag
     1321 cctgaaagcc aggataacga gcgagaaagc aaaggaaatg gcccggcttg ctgccgagga
     1381 cgccctcaag gccaagtccc cgggggcaag gaccaccacg ggtttcgttt cgcgaacggc
     1441 cacgtcgatc ccgaagctgg gagagatccc gaaggatgcc atggatctcc tgggtctcac
     1501 gaaagagaag cccgtctatg gcaagcctgt ggagatgaac ggtgattact acgtattctc
     1561 ctttgccggc gaacaggacc cggacaggaa ggaatgggaa aagaacaagg aaggtttttc
     1621 ccggtactac gcggcaaggt acagggaaca attcctgagg tccttcatcg aggaatcgaa
     1681 acaggccatg ctcaaagacg ggaagatcaa gatactcaag cccgcgaaag agctctgaca
     1741 gaatcctcgc ccatgctgat cctcggcatc gacacctcct gcgacgacac gtcggtggca
     1801 ctcatggaag agggccggaa gatccggtcc cacatcgtct ccagccaggc ggacctgcac
     1861 agtcgcttcg gcggggtggt tcccgaaata gcgtcccgaa aacacgtgga gcagatagat
     1921 gtgatatacg aagaggtcct cgcccgggcc ggggcctcgg cggaagacgt ggacctcgtc
     1981 ggtgtcaccg cgggaccggg gctcatcgga agtgtcctcg tcggcctctg cttcgccaag
     2041 gggttctgca ttgccctcga caggcccctc gtcggggtga accacataga ggcccacgcc
     2101 atgagcatct ttctcgagcg cgaggtggac ttccccttcg ttgctctcgt cgtctccggg
     2161 gggcacacga tcatcctcct catggaggcg ccctgccgtt tcagggtgct cggctccacc
     2221 cgcgacgacg ctgcgggaga ggccttcgac aagatcgcga agttcctcgg catcggttat
     2281 cccggcggga gaataataga ggagatggcc cgaagcggta tgagggacta cgtcgccttt
     2341 ccccgtccca tgatggacga caacacctgc gatttcagtt tcagcgggct caagacggcc
     2401 tttctcacct acgcgaagaa gcacaggatc gacgactcca ccatcaacca cgtcctggcc
     2461 tcctttcagg aagccatatg cgacgtcctg gtgtccaaaa cggtgagggc cgcgaggctt
     2521 gccagtgtgg aacggatcgt cgtcggagga ggcgtcgctg ccaacggcag gctccgcgag
     2581 gcctttctgg agcgctcccg gcaggagggc ctcgaggtca tgatctcgtc ccccgagttc
     2641 tgcaccgaca acggggccat ggtggcgtcg ctggcctcct tccatgccgc ggcaggcatg
     2701 tactccggtc tcgatatcaa ggggtattcg cggatggcga taaagtgatc cgacgaccat
     2761 gctgaaaagg cgtttctcgc agaacctcat aaaggacggc aacatcctgc gcaagatggt
     2821 gtccctcgcc gggatcaccg ccaaagacac ggtggtggag ataggtgccg gacagggcga
     2881 cctgacacgg gcgatcgcct cggcggcggc gcgggtcatc gccgtcgagc tcgaccggga
     2941 gatgatcccc cacctcgagg cggcgtgcct cgcttttccc aatatcgaga tcgtttccgg
     3001 cgacatactg ggcgtcgaca tcgccggtct tgcacggggc cggaccgtca aggcgatggg
     3061 gaacatcccc tacgggatca cgggacccat cattttcaag ctcatcgg
//
LOCUS       DAOPOA010000209         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_34181_length_3103_cov_2.842848, whole genome shotgun sequence.
ACCESSION   DAOPOA010000209 DAOPOA010000000
VERSION     DAOPOA010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34181_length_3103_cov_2.842848"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PKJ17_05400"
     CDS             complement(<1..399)
                     /locus_tag="PKJ17_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOD75441.1"
                     /translation="MSNEVRKEHDLLGEREIPADAYYGVQTLRATENFRITGIPLSKY
                     PAFVSSLAYVKKAAALANGELGLLPPDVADAIRRACDDVLEGKHREHFVVDMIQGGAG
                     TSTNMNANEVIANIALEKLGYEKGRYDVVHP"
     gene            complement(396..1664)
                     /locus_tag="PKJ17_05405"
     CDS             complement(396..1664)
                     /locus_tag="PKJ17_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOD75442.1"
                     /translation="MKKRWRLSQTHLIFIGMLAGLIIGFAFPALGTKMEALSTIFIRL
                     VKTIIVPIIFATLVVGIASHADLKAVGRMGVKAIVYFEIVTTIALFLGLLVVNVTKPG
                     VGIDLGDAGLPAGLGQAKHMTIKEIVINIFPENFFEAAARGDVLEVVVFTIIFAIALS
                     LIKEKKKPIIEFCEALAETMFKYTNIVMRFAPVGVGAAIAVVVASKGMSVLLNLCLLI
                     ASFYGAIVVFMLAVLLPVALLFKVPVRRFIAAVKEPAVIAFSTSSSEAALPKAMENME
                     ALGVPRRVVSFVIPTGYSFNLDGTTIYLSLASIFAAQAAGINLGLSEQLLIGFTLIFA
                     SKGAAGVPRASLVVLAGTLASFGLPIQAVAVILGVDTVMDMGRTAVNVTGNCLATVIV
                     AKWEKVFDKKEETPGEGSLDDGKTLSEASL"
     gene            1846..1919
                     /locus_tag="PKJ17_05410"
     tRNA            1846..1919
                     /locus_tag="PKJ17_05410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1880..1882,aa:Arg,seq:acg)
     gene            2325..2939
                     /locus_tag="PKJ17_05415"
     CDS             2325..2939
                     /locus_tag="PKJ17_05415"
                     /inference="COORDINATES: protein motif:HMM:NF037897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75443.1"
                     /translation="MKDPRIEIQSRTGEVVYIVDDPGQIKEVPELEYSVENPSFKEWF
                     ANSKVVDQQGNPLKVFHGTTVKSIDTFHIPAFFTDDRNGAEYFLYDEPGASIVEAYLS
                     FQNPFYACHSREESMAFIGLARRAGVEIALTESEYGWLFEAPDIEKFSPYDGTNLNHL
                     IYIPAVREQLTKEGYDSLITSDVLENGEIETYVALSSSQIWTIS"
BASE COUNT          726 a          823 c          870 g          684 t
ORIGIN      
        1 ggggtggaca acgtcgtacc gtcccttttc gtatcccagc ttctcgaggg cgatgttggc
       61 gatgacctcg ttggcgttca tgttcgtgga tgtgcctgcc ccgccctgta tcatgtcaac
      121 gacgaaatgt tccctgtgtt tcccctcgag cacatcatcg caggcccggc gtatcgcgtc
      181 cgccacgtca gggggcagga gcccgagttc tccgttcgca agggcggcgg ccttcttcac
      241 ataggccagc gaacttacaa aggcgggata tttggacagg ggaatgcccg tgatccgaaa
      301 gttctccgtt gcccgcagtg tctggacgcc atagtacgca tcggcgggaa tctcccgctc
      361 gccgagaaga tcgtgttcct tcctcacttc attgctcata gcgacgcctc cgatagtgtc
      421 tttccatcat ccagggaccc ctccccgggg gtctcctcct tcttgtcgaa taccttctcc
      481 cacttcgcga cgatgacggt ggcaaggcaa ttgcccgtga cgttcaccgc cgttcgtccc
      541 atgtccatca ccgtatccac gccgaggatg accgccacgg cctgaatggg caggccgaac
      601 gacgccaggg tgcccgcgag gacgacaagg gacgccctgg ggacgccggc ggctcccttg
      661 ctggcaaaga tcagggtaaa gcctatgagg agctgctccg acaggccaag gttgatgccg
      721 gccgcctgcg cggcaaagat ggacgcgaga gacagataga tggttgtgcc atccaggttg
      781 aagctgtatc ccgtgggaat gacgaaggag acaacccgtc tcggcacccc gagggcctcc
      841 atgttctcca tcgccttcgg cagggctgcc tccgaactgg aggtggaaaa ggcgatcacg
      901 gctggttcct tgaccgccgc gatgaacctc ctcacgggga ccttgaaaag gagcgccacg
      961 ggcaggagca cggccagcat gaagaccacg atggccccgt agaagcttgc tatgaggagg
     1021 cacaggttga ggagcacgct catgcccttg ctggcgacaa cgacggctat ggccgccccg
     1081 acgccgacgg gcgcgaagcg catgacgata ttggtgtact tgaacatcgt ctccgccagg
     1141 gcctcgcaga actcgatgat gggcttcttc ttttccttga tgaggctcag ggcgatggcg
     1201 aagatgatgg tgaagacgac cacctccaga acgtcgcccc gggcagcggc ttcgaaaaag
     1261 ttttcgggga atatgtttat gacgatctcc ttgatggtca tgtgcttcgc ctgacccaac
     1321 ccggctggca accctgcgtc accgaggtcg atgccgacgc cgggcttcgt cacgttgacg
     1381 acgaggaggc ccagaaagag cgctatggtc gtgacgatct cgaaatacac gatcgccttg
     1441 acgcccatcc tgcccaccgc cttgaggtcc gcgtggctcg cgatgcccac gacaagggtc
     1501 gcgaatatga tgggaacgat gatcgttttc acgagacgga tgaagatcgt gctgagtgcc
     1561 tccatcttcg ttcccagggc cggaaaggcg aatccgatta tcagtccggc gagcataccg
     1621 atgaagatga gatgagtctg cgagagtctc cagcgttttt tcaaaccttt cccccgtaca
     1681 aatttagtgc agtgaagcgt agcggtgcgt tgatagcggt actccgtaaa ataaatctct
     1741 tttggcgcga aaaagcaacg aaaatgcagg ccccgcgccc cttactgtgt tatcggcaaa
     1801 aggcagttga aaatacaggg caaaatcgct ataattacac accacgcgcc cgtagctcaa
     1861 ttggatagag cgttggacta cgaatccaaa ggttagaggt tcgatccctc tcgggcgcgc
     1921 ttaatatttc cagcaatatc aacaggttgc aaaggtgacc atttcccggt gacctgttga
     1981 aacccgcata attggtaaca agtggtaaca ggatgattcc cgctatccgg ttggtcccgg
     2041 aaaaatcctt gttcatagaa accgatcagt ataagatatt tctaacttat aagtaagcat
     2101 tttacgaccg ctgaggccgc taagtacctc ggtgtgacag cttcccgcgt tcgacagttc
     2161 attgtagggg agcggctcga atccgagaag cacggccccg atcatttgat cagtgagaca
     2221 gaactggagc ggtttgccag gaatggcaag aagaaacgcg gccgccccag gaaggtcaaa
     2281 taaaatgctt gcgcatgtgt tatacccggc tggtaagggc gttcttgaag gatccgagaa
     2341 tcgagattca aagcaggacg ggcgaagttg tgtatattgt cgacgatccg ggacagataa
     2401 aggaggtacc ggaattggaa tactccgttg agaatcccag ttttaaggaa tggttcgcta
     2461 atagtaaagt cgtggatcaa caggggaacc ctctgaaagt gttccacggg acgaccgtga
     2521 agagcataga cacattccac attccggctt tctttaccga cgatagaaac ggagccgagt
     2581 attttcttta tgatgagccc ggcgcctcta tagtggaagc atacctgtct ttccagaatc
     2641 ccttttatgc gtgtcactcc agagaggaat cgatggcatt cattggcctt gcccgaaggg
     2701 ctggggttga gattgcgctg acggaaagcg aatatggttg gttattcgag gccccggata
     2761 tcgagaaatt ttcgccgtat gacgggacga acctcaatca ccttatatat atcccggcag
     2821 tgcgagagca gcttacaaaa gaaggatatg attcgctgat aaccagcgat gttctggaga
     2881 atggagaaat cgagacgtat gttgccttat ccagcagtca gatatggacg atttcctgaa
     2941 atgccgactg gcgacatgtt gttgcagata attccgtttc tactgttgcc tgtcccgaac
     3001 tagcttcacg aatgaaccaa catcgagaag ccactgtctt tgttctacag tataggtgtc
     3061 gtgtaatgac ttcgggataa caagctgtaa actgttagcc ttc
//
LOCUS       DAOPOA010000210         3102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_34192_length_3102_cov_3.806695, whole genome shotgun sequence.
ACCESSION   DAOPOA010000210 DAOPOA010000000
VERSION     DAOPOA010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34192_length_3102_cov_3.806695"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>294
                     /locus_tag="PKJ17_05420"
     CDS             <1..>294
                     /locus_tag="PKJ17_05420"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75444.1"
                     /translation="WKVTVNVAMSGQREVTSVTAGSAEGSHYAIAVDIGMTTVAAQLL
                     ACDGGEGKRIILASSSDYNRQISFGEDVISRIMYAGKRNGLKKLQEVVVSTVN"
     assembly_gap    295..304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <305..1367
                     /locus_tag="PKJ17_05425"
     CDS             <305..1367
                     /locus_tag="PKJ17_05425"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOD75445.1"
                     /translation="DRDDVSHVVVAGNTTMMHLFYGIEPKHIMLAPYTPLTTVFPPVG
                     CGEVGLETAPHTRLFSVPGVSSYVGGDIVSGVALSGMTEREGVSLFMDIGTNGEIVLG
                     NGQWLLCASCSAGPAFEGGGIQFGTRAAPGAIEKVRINPATLEPMILTVGRKKPLGIC
                     GSGLIDIVAELLLAGVIDRSGKFSADRKTKRVRDGSNGREYVLCFASETNIERDIVIT
                     EIDLDNIMRTKAAVYAGCSVLLENGGLAFGDLDMVIIAGGFGHSIDIEKAIVMGLLPE
                     LPAERFRFMGNASLGGARIAAVSRGFFEKAGQVARSMTNIELSNNAQFMDEFMAAMFL
                     PHTDSRAFPETMKSLKDRQ"
     gene            1522..2292
                     /locus_tag="PKJ17_05430"
     CDS             1522..2292
                     /locus_tag="PKJ17_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD75446.1"
                     /translation="MPRVIAVAGKGGVGKTTIGGMLVRYLVEKVGKGPVLAVDADPNS
                     NLNEVLGVSVHATIGEAREKLKEDVPAGMSKDNWLEYKVQEAVIEAKGFDLLVMGRPE
                     GPGCYCAANSMAKKYIDALKGNYAFVVVDNEAGMEHMSRLVTQDVDILYVISDPTPRG
                     ILTVSRILSIIGELGLHIRKTVVIVNRVSGKDGGMLEKAARERGVEIGGIVRDDEDLV
                     RADAEGKSVFELPSASGSLVDAWAIFEGTMGPDGSAGL"
     gene            2306..>3102
                     /locus_tag="PKJ17_05435"
     CDS             2306..>3102
                     /locus_tag="PKJ17_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOD75447.1"
                     /translation="MTARIISGTEVAKQVRERIAREVLVLRERTGVTPGLVTILVGDN
                     PASVSYVTGKQRTARELGFHSVQEDQPAGISEERLLELIAKYNNDPAIHGILVQLPLP
                     KHIDETRVLNAIDPDKDVDGLHPMNLGRLMIGEARFYPCTPFGIRELLAMAGVQTKGA
                     EVVVVGRSNLVGKPISMMLVQKEANADATVTMCHTSTRDLASHTRRADILIVAAGRAR
                     AITADMVKDGAVVIDVGVNRIGVTPEGKAKLCGDVDFEAVKEKASVIT"
BASE COUNT          694 a          834 c         1004 g          560 t
ORIGIN      
        1 actggaaggt caccgtcaac gtggctatga gcggtcagag agaggtcaca tccgtcaccg
       61 cgggcagcgc cgaggggtca cattacgcga tcgccgtcga tatcgggatg acgaccgttg
      121 ccgcccaact cctcgcctgc gacggtggtg aaggaaagag gatcatactg gcatcctcct
      181 cggactacaa cagacagatc agtttcggag aggacgtcat cagcaggatc atgtatgcgg
      241 gaaagcgcaa cggactcaag aagcttcagg aggtcgtcgt ctccaccgtc aaccnnnnnn
      301 nnnntgacag ggacgacgta agccacgtcg tcgtggccgg gaacacaacg atgatgcacc
      361 tcttttacgg gatagaaccg aaacacatca tgcttgcccc ctataccccg ctcacgacgg
      421 tctttcctcc ggtcggctgc ggcgaagttg gcctcgagac tgcgccccac acgcgccttt
      481 tttccgtacc cggcgtctcg tcctacgttg ggggagacat cgtatccggt gtggcgcttt
      541 cgggcatgac ggaaagggaa ggagtttcgc tcttcatgga tatcgggacc aatggcgaga
      601 tcgttctcgg gaacggtcag tggctcctgt gcgccagctg ttcggccggt cccgcttttg
      661 agggtggagg cattcagttc ggcacgcggg cggccccggg ggccatcgaa aaggtgcgca
      721 tcaatcccgc caccctggaa ccgatgatcc tcaccgtagg caggaagaaa ccgctcggga
      781 tctgtggttc cggactcatc gacatcgtgg cggagcttct tctcgcaggt gttatcgacc
      841 gcagcgggaa gttctcggct gatagaaaga cgaagagggt gagagacggc tcgaacggcc
      901 gggaatacgt cctgtgtttc gcctcggaaa cgaatatcga acgtgacatc gtgatcaccg
      961 agatagacct ggacaatatc atgcgaacga aggccgcggt gtacgcgggc tgctcggtgc
     1021 tcctcgagaa tggaggcctt gcatttggcg atctcgatat ggttattatt gcagggggct
     1081 tcggtcactc catagacatc gaaaaggcga tcgtgatggg tcttctgccc gaactgccgg
     1141 cagagcgttt ccggttcatg gggaacgcgt ccctgggagg cgccaggata gcggccgtct
     1201 cccgcgggtt cttcgagaag gcgggacagg ttgcccggtc gatgacgaac atcgaattgt
     1261 cgaacaacgc gcagttcatg gatgagttca tggcggcaat gtttctcccc cacacggaca
     1321 gcagggcctt tccggagacg atgaagtccc taaaggacag gcaataagtg acaggtcata
     1381 ggtaataggt aataggaaga cagatgaaga cagacaatgg actacggcac acgcgccaag
     1441 cggaggttgg agaaagtttt ctcccatcac ctataaccca tcacccatca cctgcctccg
     1501 caggaggcgc aggaggcctc catgcctaga gtgatcgcgg tggcgggtaa aggcggggtc
     1561 gggaagacga ccataggcgg aatgcttgtc agatatctcg tggaaaaggt agggaagggg
     1621 ccggtgcttg ccgtggatgc cgatcccaac tccaatctca acgaggtgct gggcgtcagc
     1681 gtccacgcca ccataggaga ggccagggag aaactcaagg aggatgtccc cgcgggaatg
     1741 agcaaggaca actggttgga gtacaaggtc caggaggcgg tcatcgaggc gaagggcttc
     1801 gatcttctcg tcatgggaag gccggagggc cccgggtgtt actgcgcggc gaacagcatg
     1861 gcgaagaagt atatcgatgc cctgaagggc aactacgcct tcgtggtcgt cgacaacgag
     1921 gcgggtatgg agcacatgag ccggctcgtg acgcaggatg tggacatcct ttacgtgata
     1981 tcggacccga cgccgcgggg gatcctcaca gtgtcgcgga tcctgtcgat cataggcgag
     2041 ctcggtcttc atataagaaa gaccgttgtc atagtcaacc gcgtgagcgg gaaggacgga
     2101 ggtatgctgg agaaggccgc ccgggaaaga ggtgtcgaga taggcggcat cgtcagggac
     2161 gatgaggacc tcgttcgcgc cgacgcggaa ggaaagagcg ttttcgagct cccatccgca
     2221 tcgggttcgc ttgtggacgc ctgggccatc ttcgaaggga cgatgggtcc cgacgggagc
     2281 gctggtctgt gacgaaggag cgtatatgac ggcaaggatc atcagtggaa cggaggtggc
     2341 gaaacaggtc cgggagcgga tagcccggga ggtgctcgtg cttcgtgaaa gaacaggtgt
     2401 cacgccgggt ctcgtgacca tcctcgttgg tgacaacccc gcgtcggtga gctacgtcac
     2461 cggaaagcag agaacggcca gagagcttgg cttccactcc gttcaggaag accagccggc
     2521 aggcatatcc gaggaaagac tcctcgagct catcgcgaag tacaacaatg accccgccat
     2581 ccacggcatc ctcgtgcagc tgcccctgcc gaagcatatc gacgagacaa gggtcttgaa
     2641 cgccatagac cccgacaagg atgttgacgg tctgcatccc atgaacctgg gcaggctcat
     2701 gataggcgag gcgcgtttct atccctgcac gcccttcggc atcagggagc ttcttgccat
     2761 ggcaggtgtt cagacgaaag gtgccgaggt cgtcgtcgtc gggagaagca acctcgtcgg
     2821 caagcccatc tccatgatgc tggtccagaa ggaggcgaac gccgacgcca cagtgacgat
     2881 gtgccacacg tcgacacggg accttgcgtc acacacaagg agggcggaca tcctcatagt
     2941 cgcggcgggc agggcaagag cgataaccgc cgatatggtc aaggatggtg ccgtcgtgat
     3001 agatgtcggc gtcaacagga taggcgtgac ccccgagggc aaggcgaagc tctgcggcga
     3061 cgtcgatttc gaggcggtga aggagaaggc ctccgtcatc ac
//
LOCUS       DAOPOA010000211         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_34262_length_3098_cov_3.157739, whole genome shotgun sequence.
ACCESSION   DAOPOA010000211 DAOPOA010000000
VERSION     DAOPOA010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34262_length_3098_cov_3.157739"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            637..>3098
                     /locus_tag="PKJ17_05440"
     CDS             637..>3098
                     /locus_tag="PKJ17_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD75448.1"
                     /translation="MSITSGAAGNDLQRKEKRRVANPAGTAILAVFSFLLFAGACGAF
                     TTERHKTALFLHSYHRTDWTETIMEGYISALQGRDDLGVHVEYMDTKKTETAEYLRKL
                     RDIYAMKFGAIRFDVILVSDDNAFRFVLSHHKDLFSYAPIVFCGVNRSSRDPVAGRNN
                     VVGVLEGDDFEETLAFAFRVRPKTRTVYVVHDRTKENLKRLEELSRIIRAGYPRVTVE
                     TLGDLSYSRILEAMPTLPRDSIVFFISLWQDAEGRPVSAREASRILRLSPVPVFGRSG
                     SLVGKGLVGGKCVSGFSQGRAAGLLARQILDGKVPGDLPRVIESPNRFMFDHAELVEH
                     GIAENVLPPGSVILNKPPSFFEQNRSTILTAIAVIIVLLAFIAVLVIGLARLKRTSLK
                     LRSREQELLASQETLSGILSASPSGIVKIRGRVFEWVNDAMCSITGYEEDELVGHDSR
                     FLYPDDEEYERVGREIYRKGQEESRWVRKDGTVIELLFQVAKATGDSYIAIITDITER
                     IRTARALRESEKLYRNIIEKIQDVFYRFDINGILIMINPAGARMFRYDSVEDMLGLPM
                     EAFWNDPRDLYRMIEELKDRDRVNDYEALLRRKDGTTLYVSITTHYFLDDEGRIAGRE
                     GILRDITERKMAAEALRESEVRYRALFEFSPDAVLVLKDGVYMDCNSRTLELMKCAKE
                     DILGKGPYSFSPEIQPDGTSSIDKANEKMRLAMSGKPQFFEWRNIVLDGSGFFDVEVN
                     LSRFDVNNEVYLLVIVRDITERKRAEAEIRRLVTAIEQAAEDIFITDAEGVIEYVNPA
                     FESVTGYSRGEVLGRTPAFLTSG"
BASE COUNT          670 a          862 c          930 g          636 t
ORIGIN      
        1 gcactgcttc tatgcatcat ggtgtcgtct gccgaagcca tgggccagaa tggtgacaag
       61 tgtaccggtg aaagcgatga tccatgcgta atgcaccatt attccacctc cggattggaa
      121 tgcaatgcgc ttcaataaag cgacggtgca ggtatgcctt ctgtttcaga tcacaatcct
      181 gccatggacg atgagagcgt tatttcgagt tgcttctccc ggccgttcac ccgccgttgt
      241 aacccccgct taagaactat ggtacgttgt catcccgcaa aatgcaagcc cgattgacgg
      301 gtgagaccaa tgcggaccgc tcgagggaag accggtatga ccaccggcac ccatcctcac
      361 gggcaccgta acttctgctg ttctccgagt gctccggttc ctctccaaat ataaccggct
      421 tcgatcctgg cttctgttaa cccgctaaca ctgatggcag gcaaaccgtc attcggggtg
      481 gctctgttaa tttctcttgc ggaagtgccg ataagtatca taaggtttgt tcacagcatc
      541 gcttcagagt gctgcgttgc agggcatgag aaacgatccg cggccgtgat cgtaaattgt
      601 tcatcgagga acaggattac gcgagtatct caacgcatga gcatcacatc aggcgcggca
      661 ggtaatgacc tccagcggaa ggagaagagg cgggtagcca acccggcggg tacggcaata
      721 ctcgcggttt tctcgtttct tctcttcgcc ggcgcttgcg gggcattcac gacggagcgc
      781 cacaagacgg ctctctttct ccactcctac cacaggacag attggaccga gaccataatg
      841 gaaggctata tatccgcctt gcaggggcgc gatgaccttg gcgtgcacgt ggagtacatg
      901 gacacgaaga agacggagac cgccgagtat ctccgcaagc ttcgcgacat ctacgcgatg
      961 aagtttggcg ccatacggtt cgatgttatc cttgtctccg atgacaacgc ctttcgattt
     1021 gtcttatccc atcataagga tctcttcagc tatgctccca ttgtgttctg cggggtcaac
     1081 aggtccagcc gggatcccgt cgccggccgg aacaatgtcg tgggggtgct tgaaggggat
     1141 gatttcgagg aaacgctggc gtttgccttc cgggtgaggc cgaagacgcg aacggtctac
     1201 gttgtgcacg accggacaaa agagaacctc aagcgccttg aagaactgtc gcggataatc
     1261 agggccggct atccccgggt gacggtggag acgctgggcg acctctccta ttcgcggatc
     1321 cttgaggcca tgcccacgct tccccgggac agcatcgtgt tcttcatatc cctgtggcag
     1381 gacgccgagg gcaggcccgt ttcggcgcgg gaggcttcga ggatcctgag actgtcaccg
     1441 gtcccggtct tcggccgctc gggctcttta gtgggcaaag ggctcgttgg agggaaatgc
     1501 gtctccggtt tcagccaggg cagggccgcg ggactcctcg cgcgccagat actggacggc
     1561 aaagtccccg gggaccttcc ccgggtgatc gagagcccga accggttcat gttcgaccac
     1621 gcggaactgg tggaacacgg gatcgccgag aacgtgctcc ctccgggcag cgtcatcctg
     1681 aacaagccgc cctccttttt tgaacagaac aggagcacca tactcaccgc catcgcggtg
     1741 atcatcgttc tgctcgcgtt catcgcggtc ctggtgatcg gtctcgcgcg cctgaagcgc
     1801 acgtccttga agttgaggtc gagagagcag gaactccttg cctcacagga gacgctgtcg
     1861 gggatcctct ccgcctcgcc gagcggtatc gtgaagatca ggggcagggt ctttgaatgg
     1921 gtgaacgatg cgatgtgttc cataacgggt tacgaggaag atgagcttgt gggacacgat
     1981 tcacgattcc tctatcccga tgacgaagag tacgagaggg tgggccgcga gatctaccgc
     2041 aaagggcagg aggaatccag gtgggtccgc aaggacggta cagtcataga actcctcttc
     2101 caggtggcga aggcgacggg cgacagctac atcgccatca taacggacat aacggaacgc
     2161 atcaggacgg cccgggccct gagggagagc gagaagctct accgaaacat aatcgagaag
     2221 atccaggacg tcttctacag gttcgacatc aacggtatcc tgatcatgat aaaccccgcc
     2281 ggggccagga tgtttcgcta tgattccgtc gaggatatgc ttggcctgcc catggaggcc
     2341 ttctggaacg atccccgcga tctctacagg atgatagagg aactgaagga ccgcgaccgc
     2401 gtgaacgact atgaggcgct actaaggcgc aaggacggca cgacgctgta cgtatccatc
     2461 acgacgcatt atttcctcga tgatgagggc cggatcgcgg gccgggaagg gatcttaagg
     2521 gacatcacgg aacggaaaat ggcggcggaa gccctgcgcg aaagcgaggt gcgctaccgg
     2581 gcgctcttcg agttctctcc cgatgccgtc cttgtcctca aggacggtgt ctatatggac
     2641 tgcaactcca ggacgctgga actgatgaag tgcgcgaagg aggacattct ggggaagggt
     2701 ccttactcgt tctcgcccga gatccagccc gatggcacct cttccatcga taaggcgaac
     2761 gagaagatgc gccttgccat gtccggcaaa ccgcaatttt tcgagtggcg caacattgtg
     2821 ctcgacggga gcgggttctt cgacgtggag gtgaacctga gccgttttga cgtgaacaac
     2881 gaggtctacc tcctcgtgat cgtgcgggac ataacggaga gaaagagggc cgaggccgag
     2941 atccggcgcc ttgtcaccgc catagagcag gccgcggaag acatcttcat caccgatgcc
     3001 gagggtgtca tcgaatacgt caatccggcc ttcgaatcgg tgacggggta ttcccgcgga
     3061 gaggtactgg gcaggacccc cgctttcctc acgagcgg
//
LOCUS       DAOPOA010000212         3066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_34724_length_3066_cov_4.220525, whole genome shotgun sequence.
ACCESSION   DAOPOA010000212 DAOPOA010000000
VERSION     DAOPOA010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34724_length_3066_cov_4.220525"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PKJ17_05445"
     CDS             complement(<1..734)
                     /locus_tag="PKJ17_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOD75449.1"
                     /translation="MSTQKLKEIIKQVLPDVEAVICWQEGFDKLNVTPIFITSPEQAE
                     KVVWNPLCAQNLASYLPSQKKKVAVAVKGCDSRTIVQYMQEGLIDRNNVVIIGIPCKG
                     IISKKKVQKAIGNEPVEDVSFTEGSVKVKTPSGEKTFAIADVCPSKCTSCQYPTPIVY
                     DQLTADPIQSDKPAESVYADVAELEGKTLEERKAYFLSQFDKCIRCYACRNACPMCVC
                     QDSCIAETRDPHWITQKLSLDEKLMF"
     gene            complement(747..1163)
                     /locus_tag="PKJ17_05450"
     CDS             complement(747..1163)
                     /locus_tag="PKJ17_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOD75450.1"
                     /translation="MSEVAPKELRIVGFLCNWCSYGGADTAGVGRFRQPTDLRIIRVP
                     CSGRVDPMFVVRALLTGADGVLVSGCHPRDCHYTDGNFYARRRLEMLKRLLPFLGIDD
                     KRFHYTWVSASEGAKWQQVVTDFTKQVHALGPMHQK"
     gene            complement(1151..>3066)
                     /locus_tag="PKJ17_05455"
     CDS             complement(1151..>3066)
                     /locus_tag="PKJ17_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOD75451.1"
                     /translation="TVDVAKVAEEARKMPGVAYATAYMYTCSEPGQKEITDAIEQHKL
                     DRIVVAACSPRMHENTFRRTIQKAGLNRYFFEQANIREHVSWISLDKEANTIKAIDEV
                     RMSVAKVIKNKPLFSSSFKINKRVLVIGGGVAGMQAALDCADGGLEVVMVEKSPSIGG
                     MMARLDKTFPTVDCSICILGPKMVDVAQHDLIKLHAYSEIAEIKGYVGNYQVKIKKKP
                     SYVDWTKCTGCGLCMEKCPTKNAYDHFNFGAAPTRAINIPFPQAIPKKATIDPNYCRQ
                     FTKGKCGVCAKVCPTGAINYEMQEEFITEEVGAIITATGYGLMDIERLTEYGGGRYPD
                     VITGIQYERFLNASGPTSGHIIRPSDHEEPKTIVFVACAGSRDKSLGVPYCSNFCCMY
                     IAKQAILTKDHIPDSQSYVFYMDVRSPGKGYDEFTRRAQEEYGAKYIRGRVSRIYPKG
                     KKMVVRGADTLLGTQVEIEADLVVLGTAVVAAPGAPQMAEKLHISYDTFGFYVESHPK
                     LRPVETNTSGVYLAGACQGPKDIPASVGQGSAAAAKVLSLFSKDMLESDPAIAQVNQN
                     TCVGCLKCLKTCPFQAIVEDTLRNGKKVAKVIETVCAGCGVCTSTCPCGAIQLQHFTD
                     NQLLAEVNAICQK"
BASE COUNT          576 a          888 c          894 g          708 t
ORIGIN      
        1 tggaacatga gtttctcatc gaggctgagc ttctgggtta tccagtgcgg gtcacgcgtc
       61 tcggcgatgc agctgtcctg gcacacgcac atcgggcacg cgttccggca ggcatagcag
      121 cggatgcact tgtcgaactg gctcaggaaa tatgccttgc gctcctccag tgtcttgcct
      181 tccaactcgg caacgtcagc gtaaacgctt tccgccggtt tgtccgactg tatcggatcc
      241 gccgtaagct ggtcatagac gatcggcgtc gggtactggc acgaggtaca cttgctggga
      301 cagacatcgg ctatcgcgaa ggtcttctcg cccgacggcg tcttcacctt tacagaaccc
      361 tccgtgaagc tcacatcctc aacgggctcg ttccctatcg ccttctggac ctttttcttg
      421 ctgatgatgc ccttgcaggg gatgccgata atgacgacgt tgttcctgtc gatgaggcct
      481 tcctgcatat actggacgat ggtcctcgag tcgcagccct tcaccgcgac cgccaccttc
      541 ttcttctgcg aggggaggta gctggcgagg ttctgggcgc acagggggtt ccacacaacc
      601 ttttcggcct gctcagggga cgttatgaag atcggcgtca cattgagctt gtcaaaaccc
      661 tcctgccagc atatgacggc ctccacatcg gggaggacct gcttgatgat ctctttaagc
      721 ttctgagtac tcaatctgcc ttctccttac ttttgatgca tggggccgag cgcgtggacc
      781 tgtttggtaa agtccgttac gacctgctgc catttcgcgc cttcagaggc cgaaacccat
      841 gtatagtgga atctcttgtc gtcgatgccc aggaacggca ggagcctttt gagcatttcc
      901 aacctgcgcc gcgcgtagaa gttgccgtcg gtgtaatggc aatccctggg gtgacacccg
      961 gagacgagaa caccatcggc acccgtaaga agagcgcgca cgacgaacat ggggtcgacg
     1021 cgtcccgagc aggggaccct gatgatcctc aggtccgtgg gctgcctgaa cctgcccacg
     1081 cccgcggtgt cagcaccgcc gtaggaacac cagttacaga gaaatccaac gatccgcagt
     1141 tctttgggtg ctacttctga catattgcat taacctccgc taagagctgg ttgtccgtga
     1201 aatgctgcag ctggatagca ccgcagggac aggtggatgt acagacaccg catccggcac
     1261 agaccgtttc gatgaccttg gcgaccttct tgccgttcct caaggtgtct tcaacgatgg
     1321 cctgaaacgg acaggtcttg aggcacttga gacagcccac gcatgtattc tggttgacct
     1381 gggcaatggc cgggtcggat tcgagcatgt ccttagagaa aagcgacagg accttcgcgg
     1441 cggcggcgga accctgtccg accgatgccg ggatgtcctt ggggccctgg caggcgccgg
     1501 ccaggtagac accggaggtg ttcgtctcca ccggcctgag cttcgggtga gactcaacgt
     1561 agaacccgaa ggtatcgtag gagatgtgga gcttctcggc catctgcggg gcgcccgggg
     1621 cggcgacaac cgctgtcccg aggacgacga ggtcggcctc gatctcaacc tgggtcccga
     1681 gaagcgtatc ggcacccctc acgaccatct tcttaccctt cgggtagatc cgcgataccc
     1741 tgccgcggat atacttcgcg ccatactctt cctgggcgcg gcgtgtaaat tcatcgtaac
     1801 ctttgcccgg tgacctgacg tccatgtaga agacgtagga ctgtgaatcg gggatgtggt
     1861 ctttcgtcag gatggcctgc ttggcgatgt acatgcagca gaagttggag cagtagggaa
     1921 cgcccaggga tttatcgcgg gaccccgcgc aggcgacgaa gacgatggtt ttcggctctt
     1981 cgtgatccga cggcctgatg atatggcccg atgtcggacc ggacgcattg aggaaccgct
     2041 cgtactgaat gcccgtgatg acgtcgggat accgtccccc accgtactcg gtgagccttt
     2101 cgatatccat gaggccatat cccgttgccg tgatgatggc tcccacctct tccgtgatga
     2161 actcttcctg catttcatag ttgatggcgc ccgtgggaca gaccttcgcg cagaccccgc
     2221 atttgccttt cgtgaactgc cggcagtagt tggggtctat cgtcgctttc ttcggaatcg
     2281 cctgcgggaa ggggatgttg atggcccgcg tcggtgccgc gccgaagttg aaatggtcgt
     2341 aggcattctt cgtgggacac ttttccatgc aaagcccgca accggtgcac ttcgtccagt
     2401 cgacgtagga cggtttcttc ttgatcttta cctggtagtt gccgacatag cccttgatct
     2461 cggctatctc gctgtatgcg tgaagtttga taaggtcgtg ctgagccaca tcgaccatct
     2521 tgggtcccag gatacagatg gaacagtcga cggtggggaa ggtcttgtcg aggcgtgcca
     2581 tcatgccgcc gatggaaggg ctcttctcga ccatgacaac ctcgagaccg ccgtcagcgc
     2641 agtcgagtgc ggcctgcatg cctgccacac cgccgccaat gacaaggacc ctcttgttga
     2701 tcttgaagga cgaggagaag agcggcttgt tctttatgac cttcgccacg gacatcctta
     2761 cctcgtcaat ggccttgatg gtattggcct ccttgtcaag gctgatccag gacacgtgtt
     2821 cacggatgtt ggcctgctcg aagaagtagc ggttgagccc cgccttctgg atcgtcctcc
     2881 tgaaggtgtt ctcgtgcata cggggtgagc atgccgcgac aacgatcctg tcgagtttgt
     2941 gctgctcgat agcatccgtg atctctttct gtccgggctc tgaacaggta tacatgtagg
     3001 ccgtcgcata ggcgacgccg ggcatcttcc gggcttcctc ggccactttc gcgacgtcaa
     3061 cggttc
//
LOCUS       DAOPOA010000213         3063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_34787_length_3063_cov_3.709774, whole genome shotgun sequence.
ACCESSION   DAOPOA010000213 DAOPOA010000000
VERSION     DAOPOA010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34787_length_3063_cov_3.709774"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKJ17_05460"
     CDS             complement(<1..401)
                     /locus_tag="PKJ17_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75452.1"
                     /translation="MNILVFTKRVAATQEEELRITGEGQAVDLSKVPFKVNDWDNYSV
                     EEAVRIVDKAGGSVTAISIGDAESDEVLRRAIAMGAKDGFLVETGDVLNDPFMRGELI
                     RSFIDKEKVPFDIILTGVQSEDDQFGAVGGIV"
     gene            complement(403..1497)
                     /locus_tag="PKJ17_05465"
     CDS             complement(403..1497)
                     /locus_tag="PKJ17_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOD75453.1"
                     /translation="MVEQAATRVLTCIQCGKCTGSCPESGKTPFNVRMLVRKKQFQSA
                     IDEALPWYCTSCGACTIRCPRDVKPSEVIIELRAKLVEDGDIPVALQKALENTFVQKN
                     PWGRSRNKRADWAQKLDFEVPHVSETESKRLLFVCCIQAYDPRCMVIPVNVARLFNAG
                     GLEFGILGEEEACCGNEIRRIGEAGLFEELEEENKAAFEEYGVKEIVTLSPHCMNVLK
                     KEYGDMGIKVVHYTELLAPLVEEGAIKLPGSFGKKVIYHDPCFLGKQNGVFDEPRAIL
                     GAVPDLELLEFSRSREMSLCCEGGGGRMFFEVEATYQRNADVRVLDAVSRGAEIIATA
                     CPFCVMTLEDPATEKGLAVKELSEILTEVL"
     gene            complement(1893..3038)
                     /locus_tag="PKJ17_05470"
     CDS             complement(1893..3038)
                     /locus_tag="PKJ17_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD75454.1"
                     /translation="MELTTEQKDICKAAREFAEKEFRDKAKEFDEKEEFDLSIVKRAA
                     ENGFIGVFIKEDYGGAGLGFLEHSLITEEVWRVDPGCGQNICSAGFGAEMIQTFGTEE
                     QKQFYLPKIASGEAIIGTAITEPDAGSDVTMVKTRAEKKGDRYVINGAKMFITNGCNA
                     KYLLVYAVTDPEAKDVHKRCSVILLDTATPGFAATKIYGKMGIRASNTAEVSFDNVEV
                     PAENLIGKEGRGFYQLMEFFNMTRNHVAAQGVGVAQGALEMAISHVKKRKAFGGSLSR
                     LQGVQFKIAEMATRIEAARSLYWRAAYLLDNGKLDPALVSMAKWFSGETAVYVANEAL
                     QLHGGYGYIIEYDIQRFYRDAKIVEIYEGSKEVEKAVIASELLKRVF"
BASE COUNT          572 a          936 c          877 g          678 t
ORIGIN      
        1 acgatacctc cgacggcccc gaactggtcg tcttccgact gcacacccgt caggatgata
       61 tcaaagggga ccttctcctt gtcgatgaag ctgcggatga gctcgcccct catgaagggg
      121 tcgttgagga catcccccgt ttccacgaga aagccgtcct tcgcccccat ggcgatggcg
      181 cgccgcagaa cctcgtcgga ttcggcgtca ccgatggaaa tggccgtcac cgaaccgccc
      241 gctttatcga cgatgcgcac cgcctcttcc acgctgtagt tgtcccagtc attgaccttg
      301 aagggtacct tcgagagatc gacggcctgc ccttccccgg tgatgcgcag ctcttcttcc
      361 tgggttgcgg caaccctctt cgtaaataca aggatattca tcttataaga cctccgtaag
      421 tatttctgaa agctccttca cggcgagacc tttttctgtg gccgggtctt ccagggtcat
      481 gacgcagaag gggcatgccg ttgcgatgat ctcggccccc ctgctgacgg catcgaggac
      541 gcggacgtcg gcgttgcgct gatacgtcgc ctcgacctca aagaacatcc ttcctccccc
      601 gccttcgcaa cacagtgaca tctcccgcga gcgggagaac tcgagaagct cgaggtcggg
      661 gacggcgccg aggatcgcgc gcggctcatc gaagacaccg ttctgtttcc cgagaaaaca
      721 ggggtcgtgg tagatgacct tcttcccgaa agaccccggc agcttgatcg ccccctcttc
      781 gacgaggggc gcgagaagct ccgtgtaatg gacaaccttg atgcccatgt cgccgtactc
      841 tttcttcagg acgttcatgc agtgcggcga gagggtgacg atctccttga caccgtactc
      901 ctcgaaggcg gccttgttct cctcctcaag ctcttcgaag aggcccgcct cgccgatacg
      961 ccggatctcg ttgccgcagc acgcctcctc ttcaccaagg atgccgaact cgaggccgcc
     1021 ggcgttaaag agccgcgcga cattcacggg aatgaccatg cacctcggat cgtatgcctg
     1081 aatacagcac acgaagagga gccttttgct ttccgtttcc gatacgtggg gaacctcgaa
     1141 atcgagtttc tgcgcccagt cggcacgctt gttgcgggag cgcccccagg ggtttttctg
     1201 cacgaaggta ttctccaggg ccttctggag cgcgacgggt atgtcaccgt cttcgacgag
     1261 tttcgcgcga agctcgatga tgacctccga gggcttcacg tccctcgggc accgtatggt
     1321 acaggcgccg caggacgtgc agtaccatgg aagggcctcg tcgatggcag actggaactg
     1381 tttcttcctg acgagcatcc tcacgttgaa aggcgtcttg cccgattcgg ggcaactccc
     1441 cgtgcacttc ccgcactgga tgcaggtcaa gaccctggtt gcggcttgtt caaccattat
     1501 acgtaacccc cgatggtgat tccatttgta tggcggtagt tgttctccat gtctccgtgc
     1561 aaggatgtat gcaatgggga taccaaagca agccgggcag cccgtcgcca attattatgg
     1621 tatgttagcc caaacgtggc acaggatgca acaccccccg cgtgtccggt ttgggaacag
     1681 gtgtaacctt ttgatacagg cctggggagg atcagatcgt tgagatcgtt tgagagaagg
     1741 gggtgacgaa tcggagtgga aacccacttt gcaaacaggc tgcagggcgt tggaaaaaaa
     1801 acaatgaccc ttgcgtgacc gcgcacgaca ggggccattg ttgtctttaa acgttgaaca
     1861 ggctcaagcc attcaagcgg ttcaagccgc gtctagaaga cgcgtttcag gagctcgctg
     1921 gcgatgaccg ccttctcgac ctccttggag ccttcgtaga tctcgactat cttcgcgtcg
     1981 cggtagaacc gctgaatatc gtactcgatt atgtagccgt agccgccgtg gagctggagg
     2041 gcctcgttgg ccacgtagac ggcggtctcg ccggagaacc acttggccat tgagacgagc
     2101 gccgggtcga gcttgccgtt gtccaggagg tacgcggcgc gccagtagag actgcgggcg
     2161 gcctcgatgc gggtggccat ctcggctatc ttgaactgca cgccctggag ccgcgagagg
     2221 gatccgccga aggcctttct cttcttcacg tgggagatgg ccatctcgag ggcgccctgg
     2281 gcgacgccaa caccctgggc ggcgacgtgg ttgcgggtca tgttgaagaa ctccatgagt
     2341 tggtagaagc cccggccttc cttgccgatg aggttctcgg cggggacctc cacgttgtcg
     2401 aagctcacct ccgcagtgtt cgaggccctg atgcccatct tgccgtagat cttcgtcgcc
     2461 gcaaaaccag gggtcgccgt atcgaggagt atgacgctgc accgcttgtg cacgtccttc
     2521 gcctcgggat cggtgacggc gtaaacgaga aggtatttcg cgttgcagcc gttcgttatg
     2581 aacatcttcg cgccgttgat gacgtacctg tctcccttct tctcggcgcg ggtcttcacc
     2641 atcgtcacgt cactgcccgc gtcgggctcc gttatggcgg tgccgatgat ggcctccccg
     2701 gaggcgatct tcgggagata gaactgtttc tgctcctccg ttccgaaggt ctggatcatc
     2761 tcggcgccga aaccggcgga acagatattc tggccgcagc cggggtcgac acgccacacc
     2821 tcttcggtga tcagcgaatg ctcgaggaac cccaggcccg ctccaccgta gtcctccttg
     2881 atgaagaccc cgatgaagcc gttctcggcg gcgcgcttca cgatggacag gtcgaactcc
     2941 tccttctcgt cgaactcctt cgccttgtcc ctgaactcct tctcggcaaa ttcacgggcc
     3001 gctttgcata tgtccttctg ttccgttgtc agttccatct gtcttcctcc aaaaggttat
     3061 tcc
//
LOCUS       DAOPOA010000214         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_34882_length_3056_cov_3.008997, whole genome shotgun sequence.
ACCESSION   DAOPOA010000214 DAOPOA010000000
VERSION     DAOPOA010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34882_length_3056_cov_3.008997"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="PKJ17_05475"
     CDS             complement(<1..1584)
                     /locus_tag="PKJ17_05475"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOD75455.1"
                     /translation="MMINITIDGRPVEVEEGTTILNAAAKAKVRIPTLCYLPEVQAIG
                     ACRVCLVEVEGNRTLQAACVAPVANGQVIHTNTERVRKARRFSVEMLLSNHPFECLSC
                     AKNLHCDLQSLAYDLGIREIRFSGEKSGGRIDESSPSIRRDQNKCILCRRCVTVCQEI
                     QSVTALYMQGRGFETRVEPAFDRDINDVACTNCGQCSIVCPVGAITEKEYIDDVWAAL
                     NDPTKFVVIQDAPAVRAALGEEFGYPPGTLVTGKMLAAARRLGFDRVFDTNFAADLTI
                     LEEGTELLTRVKKGGVLPMITSCSPGWIKFIEHFYPELLDHLSTCKSPHMMLGALAKT
                     YFAQKVGIDPKDIVVVSVMPCTAKKFECNRPEMYDSGYKDVDYVLTTREFAKMIKQAG
                     IDFEGLPDESYDDPLGEYTGAATIFGATGGVMEAALRTAYEVATGKTLESVEFTGVRG
                     LDGIKEAMVPVDGIGDVRVAVAHGLGNARKLLERIKKGEANYHFIEVMACPGGCVGGG
                     GQPIPVNNEIRSLRAQALYN"
     gene            complement(1616..>3056)
                     /locus_tag="PKJ17_05480"
     CDS             complement(1616..>3056)
                     /locus_tag="PKJ17_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD75456.1"
                     /translation="LFDAPCQNACPADQNAWGYVTLISEGRMKEAIAVIKESNPFPAT
                     LGRVCVHPCEAKCRRAQIDAPISICSLKRYAADVDMKSHPRYKPPVEEPKGKKVAVIG
                     AGPAGLAGAYFLALKGYGVTVFEALPVAGGMLAVGIPDYRLPRDILESEVKSITDLGV
                     EIKYNMAFGKDITAEKLFGEGYSAILVATGAHKGQKLNVPGEETPGVLDGVTFLRDVN
                     LGKTLKVEGKVAVIGGGNVAIDAARAALRLGANEVSILYRRERSDMPAYVEEIDEAEN
                     EGIAIHTLTAPLSIVAKGGKAAGVECSRMSLGKFDKGGRRTPQAIPGSDFVVDADMVI
                     AAIGQIPDLSWMNGDGVKADRAGTVEVNMKTLATGAAGIFAAGDNVRGPATVVEAVGD
                     GKMAAMAIDEYLGGDGKAPNAFRDELVNMVVSYDEAAYQKELGRADMPHLPVSKRERN
                     FNEVVLGYDERAAVEEAKRCLHCYVRQTE"
BASE COUNT          530 a          974 c          886 g          666 t
ORIGIN      
        1 gttgtacagc gcctgtgccc tgagcgaccg gatctcattg ttgacgggga tgggctggcc
       61 gccgccgccg acgcatccgc cggggcacgc catgacctcg atgaaatgat agttcgcctc
      121 ccccttcttg atcctctcga ggagcttgcg ggcattgccc aacccgtgcg ccacggcgac
      181 ccgcacgtca cctatgccgt caacaggaac cattgcctcc ttgatcccgt cgagacccct
      241 cacgcccgtg aactccacgc tttcgagggt cttgcccgtc gccacttcgt aggccgtgcg
      301 aagcgcggcc tccatgaccc cgcccgttgc cccgaagatc gtcgcggccc cggtgtattc
      361 tcccaggggg tcgtcgtaac tttcatcggg aaggccctca aagtcgatgc cggcctgctt
      421 gatcatcttg gcgaattccc gggtcgtcag gacgtaatcg acatccttgt aaccgctgtc
      481 gtacatctcg ggacggttgc actcgaattt ctttgccgtg cagggcatga cggaaacgac
      541 cacgatgtct ttcgggtcaa tgccgacctt ctgcgcgaaa taggtcttcg ccagggcgcc
      601 aagcatcatg tgcggcgatt tacatgttga cagatggtcg agaagttcgg ggtagaaatg
      661 ttcgatgaac ttgatccatc ccgggctgca gcttgttatc atcggaagga cgccgccctt
      721 cttgacccgc gtgagaagct ccgtgccttc ctcgaggatg gtgaggtcgg cggcgaagtt
      781 cgtgtcgaag acccggtcga aacccagcct gcgggccgcg gcgagcatct tacccgtcac
      841 cagcgtgccg ggaggatatc cgaactcttc acccagggcc gccctgaccg cgggcgcgtc
      901 ctgaatgacg acgaacttgg tcgggtcgtt gagcgccgcc cacacgtcgt ctatgtactc
      961 cttttccgtt atggccccga cgggacagac gattgagcac tggccgcagt tcgtgcaggc
     1021 cacatcgttg atgtcgcggt cgaaagcggg ctccacgcgt gtttcgaagc ccctgccctg
     1081 catgtagagg gccgtcacgg actgtatctc ctggcagacg gttacacatc gcctgcagag
     1141 aatgcatttg ttctggtccc gccgtatcga cggagatgat tcatctatcc ggccgccgct
     1201 cttttccccg ctgaagcgaa tctccctgat gccgagatcg tatgcgagcg actgaagatc
     1261 gcaatgaagg ttcttcgcgc aggaaaggca ctcgaagggg tggttcgaca gcagcatctc
     1321 caccgagaag cgcctcgcct tgcggacccg ctctgtattg gtgtgaatga cctgcccgtt
     1381 ggccacgggc gcaacgcatg cggcctggag ggtccggttg ccttccactt cgacaaggca
     1441 aaccctgcag gcgcctatcg cctggacctc gggaaggtag cacagggtcg gtatgcggac
     1501 ttttgccttc gcggcggcgt tgagtatcgt tgttccttct tcgacttcga cgggtcggcc
     1561 atcgatcgta atatttatca tcatagtggt ctactccttg agtcggttac ccgatttact
     1621 cggtctgcct gacgtagcag tgaaggcatc tcttggcctc ttcgactgcg gctctttcgt
     1681 cataaccgag aacaacctca ttgaaattgc gttccctttt cgatacaggc agatgcggca
     1741 tatccgccct gccaagttcc ttctggtacg ccgcctcgtc ataggagacg accatattga
     1801 cgagttcgtc gcggaaggca ttcggggcct ttccgtcacc gccgagatat tcatcgatgg
     1861 ccatggcggc catcttgccg tcgccgacgg cctcgaccac ggtggcaggt cccctcacat
     1921 tatcgccggc ggcgaagata ccggcagcgc ccgtggcgag ggtcttcatg ttcacttcca
     1981 cggtccctgc cctgtctgcc ttgacaccat cgccgttcat ccacgaaaga tcgggtatct
     2041 ggcctatagc ggctataacc atgtcggcgt cgacaacaaa atcagacccg ggtatggcct
     2101 gcggtgtgcg cctgccgcct ttgtcgaact tcccgaggct catccgggag cactcgaccc
     2161 ccgctgcctt tccgcctttc gccactatgc tcaaaggtgc cgtgagggtg tgaatggcga
     2221 tgccttcgtt ctcagcctcg tcgatctcct caacgtatgc gggcatatcc gaccgttcgc
     2281 gtctgtagag tatggaaacc tcattcgccc ccagcctgag ggccgcccgt gccgcgtcga
     2341 tggccacgtt gccgccgccg atgacggcga ccttgccttc gaccttcaac gttttgccaa
     2401 gattgacgtc gcgcaggaat gtcacgccgt caagcactcc cggggtttcc tcgccaggga
     2461 cgttgagctt ctgccccttg tgagcgcccg tcgccacgag gatggcactg tatccctcgc
     2521 cgaagagctt ttcggcggta atgtccttgc caaatgccat gttgtacttg atctccacac
     2581 cgaggtcggt gatggacttg acctcgctct ccagtatgtc cctgggcaga cgatagtcgg
     2641 ggatacccac ggcgagcatg ccacccgcca cggggagcgc ctcgaagacc gtcacgccgt
     2701 accccttgag ggcaaggaaa taggctcccg ccagcccggc cgggccagcg ccgatgacgg
     2761 cgaccttctt cccctttggt tcctccaccg gaggtttgta gcgggggtgg gatttcatgt
     2821 ccacgtccgc ggcgtaccgc ttcagcgagc agatcgagat gggagcgtct atctgcgccc
     2881 tgcggcactt cgcctcgcag ggatggacac agacccggcc cagcgtcgcc gggaagggat
     2941 tcgattcctt gatgacggcg atggcctcct tcatccggcc ttcgctgatg agggtcacgt
     3001 atccccaggc attctggtcc gcgggacagg cgttctggca gggggcgtca aagagc
//
LOCUS       DAOPOA010000215         3040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35108_length_3040_cov_2.917253, whole genome shotgun sequence.
ACCESSION   DAOPOA010000215 DAOPOA010000000
VERSION     DAOPOA010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35108_length_3040_cov_2.917253"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PKJ17_05485"
     CDS             complement(<1..357)
                     /locus_tag="PKJ17_05485"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOD75457.1"
                     /translation="MRLVVFNGSPRVGGNTDLLVASVLKGIDRHLHRVDLFRLNEMNI
                     RPCQNCGGCTDTGVCVLKDQMQQVYPALRKADRVILASPIYFSGLSAQTKTMIDRCQP
                     LWCEKYIHNMPLHGSVH"
     gene            complement(421..915)
                     /locus_tag="PKJ17_05490"
     CDS             complement(421..915)
                     /locus_tag="PKJ17_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOD75458.1"
                     /translation="MSKTQKNLEEAFAGESQANRKYLAFAKKADEEGFKQVAKLFRAA
                     AEAETIHAHNHLRELKGIKSTKENLMEAVGGETHEFKSMYPAMMEEAKAEGNTGAVRT
                     FQFANDVEIVHAGLYQKALDSLGNNEDTDYWVCQVCGNTVEGEPPDECPVCKAKKVAF
                     YKVA"
     gene            complement(949..2172)
                     /locus_tag="PKJ17_05495"
     CDS             complement(949..2172)
                     /locus_tag="PKJ17_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOD75459.1"
                     /translation="MKPIEIKPGIYWVGVIDWAIRDFHGYVTKNGTTYNNYLVMDDEV
                     TLLDTVKHDFDATAVENIRRVVDPYKIKNIVINHIEPDHASSIAAIMEYAPDATIFIT
                     ERGKKGLDRHFDTSRWKFRIVKSGDTLKTGKYTLTFLETPMLHWPDSMVTYIPEAKLL
                     ISQDAFGQHMASAARFDDEFFSCHSQFELDDAIVDYYANILMPFGKLIEAKIQEIVKL
                     GLEIDMIAPDHGIIWRKDPGYIIQRYLDLAKGKADLAAVIIYDTMWHSTELMSDPIMN
                     GIKDEGVPCKVIKLRSTSMSEAIKEFWRARGCLIGTPTINNVMFPSVAEFLYHLAGLR
                     PKDRIVGGFGSYGWGGGAVKEAYAKFQQMGLEAVEPGLQVLYKPSAEDMDRCYEFGRD
                     FAKRLKEYHGKFQAV"
     gene            complement(2349..>3040)
                     /locus_tag="PKJ17_05500"
     CDS             complement(2349..>3040)
                     /locus_tag="PKJ17_05500"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD75460.1"
                     /translation="FQEKTSKDLDNALKELEKSNKGKPLKGLVLDLRNDPGGLLEQAV
                     EVTDKFLSEGVIVSIQGRGGKKAPDDSKMKFYAQKKGQEFLGPMVVLVNEGSASASEI
                     VAGALQDYKRAVIVGTKSFGKGSVQTIFPLGDGSAVRLTTAKYFTPKGRSIQSEGIVP
                     DITVENTFVRSREKLAPITEKDLEKKNDRSPMPRRPEDITIKNLEDEDFQLYMGLQIL
                     KSWEALRGKAS"
BASE COUNT          573 a          928 c          783 g          756 t
ORIGIN      
        1 atgcacgctc ccatggagag gcatgttgtg gatatacttc tcgcaccaca ggggctggca
       61 gcggtcgatc atggtcttcg tctgggccga tagcccggag aagtagatag gcgaagcaag
      121 gatcaccctg tccgctttcc tgagggcggg atacacctgc tgcatctggt ccttgaggac
      181 gcataccccg gtatccgtgc accctccgca gttctgacag ggtctgatgt tcatctcgtt
      241 gagacgaaag aggtccacgc ggtgcaggtg ccggtctata cccttcagga ccgacgcgac
      301 gagaaggtca gtgttgcctc ctaccctggg acttccatta aatacaacga gtctcaccta
      361 acctccatga aaagggcgag agggggggcc gcctcacccc tgccctcccg gataccgata
      421 tcaggccact ttgtagaagg ccaccttctt cgccttgcag accggacact cgtcgggcgg
      481 ctccccctcc acggtgttgc cgcacacctg gcagacccag tagtccgtgt cctcgttgtt
      541 gccgaggctg tcgagcgctt tctgatagag accggcgtgc acgatctcaa catcgttggc
      601 aaactggaag gtcctcacgg cgcccgtgtt tccctccgcc ttcgcctctt ccatcatcgc
      661 cgggtacatg gatttgaact cgtgggtctc accaccgacc gcctccatga ggttttcttt
      721 cgtgctcttt atccccttca gttccctcag atgattgtgg gcgtggatgg tctccgcctc
      781 cgccgccgcc ctgaaaagct ttgccacctg tttgaaacct tcttcatccg ccttcttcgc
      841 gaaggcgaga tacttgcggt tggcctgcga ttcgccggca aatgcttcct cgagattctt
      901 ctgcgtcttt gacatggtcc ctccttctga tattattcac acctatgatc agaccgcctg
      961 gaacttcccg tggtactcct tcaaacgctt cgcaaagtcc cggccgaact cgtagcacct
     1021 gtccatatcc tcagcggacg gtttgtacag cacctggagg cccggttcca ccgcttcgag
     1081 acccatctgc tgaaatttgg cgtaggcctc cttgacggct ccgcctcccc atccataact
     1141 gccgaacccg ccaacgatcc tgtccttcgg tctcaaaccc gccaggtgat acaggaactc
     1201 cgcgacggat gggaacatga cattgttgat ggtcggggtc ccgatcaggc agccccgcgc
     1261 cctccagaac tcctttatcg cctcgctcat ggatgtcgac ctcagtttga tgaccttgca
     1321 gggcacccct tcgtccttga tgccgttcat gatggggtcg ctcatgagtt ccgtcgaatg
     1381 ccacatcgta tcgtagatga tgacggccgc gaggtctgcc ttgcccttcg ccagatccag
     1441 gtaccgctgt atgatgtaac cggggtcctt cctccatatg atcccgtgat cgggcgctat
     1501 catgtcgatc tcgagcccga gctttacgat ctcctggatc ttcgcctcga taagctttcc
     1561 gaaaggcatg aggatgttag cgtaataatc cacgatggca tcgtcgagct cgaactggga
     1621 gtggcaggaa aagaactcgt cgtcgaacct ggcggccgag gccatgtgct gaccgaaggc
     1681 gtcctgcgat atgaggagtt tcgcttcggg tatgtaggtc accatggaat ccggccagtg
     1741 cagcatgggc gtctccagga acgtcagggt gtacttcccc gtcttcaggg tatcgccgct
     1801 tttgactatc ctgaatttcc agcgcgacgt gtcgaaatgg cggtccagac ccttcttccc
     1861 cctctccgtg atgaagatgg tcgcgtcggg ggcatactcc atgatcgccg cgatgctgct
     1921 ggcgtggtcc ggttcgatgt gattgatcac gatgttcttt atcttgtacg gatccaccac
     1981 gcgccgtatg ttctcaacgg ccgtggcgtc gaaatcatgc ttgacggtat cgagaagggt
     2041 cacctcgtca tccatgacaa gatagttatt gtatgtggtg ccgttcttcg tgacataccc
     2101 atgaaaatcc cggatggccc aatcgatcac cccaacccag tagatacccg gtttgatctc
     2161 tatcggcttc atgtatcctc cttttcggta tgatggaaaa cgctttgctg acgtaccatt
     2221 cttttttgac gtactaaaag ataaaacctc gccgggaaaa agacaagcac aggatgctat
     2281 tctacccata aaaaaaacgg gtaagaccaa tgacggtcca cccgttcctg tccgccgtga
     2341 gggggcttct acgaggcctt gccccttaag gcctcccagc tcttgaggat ctgcaggccc
     2401 atgtaaagct gaaaatcctc gtcttcgagg ttcttaatgg tgatatcttc gggcctcctg
     2461 ggcatcggac tcctgtcgtt cttcttctcg aggtcctttt ccgtgatggg cgccagcttc
     2521 tctctcgacc tcacgaaggt gttctccacg gtgatgtcgg gaacgatgcc ttccgattgg
     2581 atggaccgcc ctttgggcgt gaaatacttc gctgtcgtca accgcacggc ggacccgtca
     2641 cccagaggga atatggtctg caccgatccc ttaccgaagc ttttcgtgcc aactatgacg
     2701 gcccgtttgt aatcctgaag ggcccccgcg acgatctccg acgcgctggc gctgccctcg
     2761 ttgacaagca cgaccatggg ccccaggaat tcctggccct ttttctgtgc atagaacttc
     2821 atcttgctgt catcgggagc tttcttgcct cccctgccct gtatggaaac gatgacgcct
     2881 tcggagagga acttgtccgt cacttccacc gcttgttcga gaagcccgcc gggatcgttc
     2941 ctgaggtcca gcaccaaccc tttcaaaggt ttgcccttgt tgctcttctc aagctctttg
     3001 agtgcattgt ccaggtcctt cgacgtcttc tcctggaact
//
LOCUS       DAOPOA010000216         3037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35162_length_3037_cov_3.129443, whole genome shotgun sequence.
ACCESSION   DAOPOA010000216 DAOPOA010000000
VERSION     DAOPOA010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35162_length_3037_cov_3.129443"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PKJ17_05505"
     CDS             <1..497
                     /locus_tag="PKJ17_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD75461.1"
                     /translation="DLGIAFAAVLILIYVMVVAWFRSFTIPLVIMAPIPLSLIGIIPG
                     HAIMGAFFTATSMIGFIAGAGIVVRNSIILVDFIDLKLAEGIPLEEAVVEAGIVRFRP
                     MLLTAAAVVVGSSVILFDPIFQGMAISLMSGEVAATFLSRTMVPVLYFLLKRHENEKA
                     SLLI"
     gene            600..2021
                     /gene="fumC"
                     /locus_tag="PKJ17_05510"
     CDS             600..2021
                     /gene="fumC"
                     /locus_tag="PKJ17_05510"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOD75462.1"
                     /translation="MFDEPDGTVRNGLRLESDSMGKVWVKADRLWGAGTQRALVHFRI
                     GEDLNPAELTRAIVIVKQAAAKANRDLGKLDEGRAKAILLAAEEAVVGKLDSHFPLPV
                     WGSGSGTQLNMNVNEVLANRAIQILDGTVGTKTPVHPNDHVNMSQSTNDVFPTAMNVA
                     ASLAIRDHLVPMVEKLRDGLDEKTKAWANITKIGRTHLMDAVPLTLGQEFSGYAAMVD
                     DDLTRLEAVLPGLYRLAIGGTAVGTGLTAEKRFGELAVRHIAEITHLPVTAAPNRFAV
                     QGAHDAMVMVSGALKTMAVSLFKIANDIRLLASGPDSGLHELILPANEPGSSIMPGKV
                     NPTQCEAMTMVAVQVMGYDAAVAIAGAGGYLEMNVYKPLIAFNVIQSIRLISDACSSF
                     TEFLVAGMKPNRKRIDEYVNRSLMLVTALVPSIGYDRAAKVAHLAAGEGLTLKEAALK
                     LGYISADEFDRIVDPGKMVDPDG"
     gene            2052..>3037
                     /locus_tag="PKJ17_05515"
     CDS             2052..>3037
                     /locus_tag="PKJ17_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOD75463.1"
                     /translation="MILDHDAVIVGSGLAGLRAAVEATETAGVAVVSKVLPTRSHSGA
                     AQGGITAALGNEEEDRWEWHLFDTVKGGDYLGDQDAIEIMVRDAPRAIFELEHMGVPF
                     SRNPDGRIAQRNFGGHTRQYGKAPVRRACHAADHTGRVVLDTLYDQAMRQNVKFYAEY
                     YVLSLVFDEGRCTGVVTYNLATGELDLLRAKAVILATGGCGKIYRTTSNGFATTGEAL
                     SLVYREGIPLEDMEFVQFHPTGLFPLGILVSEAARGEGGILRNDAGEPFMERYAPAIK
                     DLAARDVVSRAILTEIRAGRGIGGKAYVHLDLTHLGERKITEKLWEITSFAR"
BASE COUNT          645 a          845 c          909 g          638 t
ORIGIN      
        1 gcgacctggg gatagcgttt gccgcggtcc tcatcctgat ctacgtgatg gttgtcgcat
       61 ggttcaggtc ttttacgata ccgctcgtca taatggcgcc cataccgctt tctcttatcg
      121 gcataatacc ggggcacgct attatgggag ccttcttcac tgcaacatcc atgataggct
      181 ttatcgcggg ggccggcatc gtcgtgagga attcgatcat cctggttgat ttcatagact
      241 taaagttagc tgagggaata ccgctcgagg aagctgttgt cgaagctggg atcgttcgtt
      301 tccggcccat gctcttgaca gccgccgccg ttgtggtagg gtcctcggtc atacttttcg
      361 atcctatctt tcagggaatg gcgatttctc tcatgtcagg tgaggtcgcg gcaacttttc
      421 tttcccggac aatggtcccg gtcctctatt ttctcctgaa acgacatgaa aacgagaagg
      481 cgtcccttct tatctgaaat atctcgatga tgtattatat taattttaga cgcaagaaaa
      541 gtcttctctt gggtcgcgtt tcggcagtac agacaatttg acaggaaggt ccgaaagcca
      601 tgttcgatga acctgacgga accgtaagga atggcctgag actggaaagc gacagtatgg
      661 ggaaggtgtg ggtcaaggcc gaccgcctct ggggtgctgg cacgcagcgg gccctcgtgc
      721 acttcaggat cggcgaggac ctgaaccctg cagagcttac cagggccatc gtcatcgtga
      781 aacaggctgc ggccaaagcc aatcgggacc tcggaaagct cgatgaagga agggcgaaag
      841 ctatcctcct ggctgccgaa gaagccgtcg ttggcaaact ggacagtcat ttccccttgc
      901 ctgtctgggg cagcggcagc ggcacccagt tgaacatgaa cgtcaacgag gtccttgcga
      961 accgggccat ccaaatcttg gacgggaccg tggggaccaa aactccggtc catcccaatg
     1021 accatgtcaa catgtcgcag tcgacaaatg acgtctttcc cacagctatg aatgtcgccg
     1081 cctctctcgc tattcgggac catctcgtgc cgatggtgga gaaactgcgg gacggcctcg
     1141 atgaaaagac gaaggcgtgg gccaacatca cgaagatcgg aaggacccac ctgatggatg
     1201 cggtgccact caccctgggc caggagtttt ccggttacgc cgccatggtc gatgacgatc
     1261 tcacgagact cgaagcagtg cttcccggcc tctatcggct cgccatcggc ggtacggcag
     1321 tgggtaccgg cctgaccgct gaaaagagat tcggggaatt ggcggtgcgc cacattgccg
     1381 agatcacgca cctcccggtg acggcagctc cgaacaggtt tgccgtgcaa ggtgcccacg
     1441 atgccatggt catggtgagc ggtgccctga agaccatggc tgtctccctt ttcaagatag
     1501 ccaacgatat ccggctcctc gcctcggggc ccgactcggg ccttcacgag ttgatccttc
     1561 ccgccaacga acccgggtcc tcgatcatgc ccggcaaggt aaacccgacc caatgcgagg
     1621 ctatgaccat ggtcgctgtg caggtgatgg ggtacgacgc ggcagtggcc atcgccggtg
     1681 ccggcggtta tctggagatg aatgtctata agccgctcat agccttcaac gtcatccagt
     1741 ctatccgtct tatctccgac gcctgcagtt catttacgga attcctggtt gccggtatga
     1801 agccaaacag gaagcggata gacgaatacg tgaaccgctc gctcatgctc gtcacagccc
     1861 ttgtgccgag catcggttac gatagggccg caaaggtcgc tcacctggct gccggggagg
     1921 gccttacgct taaagaagca gctctgaagc tcggctatat ttcggccgat gagttcgatc
     1981 gcatagtcga tcctggcaag atggtggacc cggatggatg atgcggtttt attgtcggag
     2041 gtattcgtca catgatcctt gaccatgacg cagtgatcgt cggctcgggg cttgcgggcc
     2101 tcagggccgc cgtggaagct acagaaacag cgggggttgc tgtagtatcc aaggttttac
     2161 ccacgcgttc ccattcaggg gcagcccagg gagggatcac cgccgccctc ggcaatgaag
     2221 aagaagaccg gtgggaatgg catctctttg acacggtcaa gggcggcgat tacctggggg
     2281 accaggatgc catcgagatc atggtaaggg acgcgccgag ggcgatcttt gaactagaac
     2341 atatgggggt tcccttcagc cgcaacccgg acggcaggat agcccagagg aacttcggag
     2401 gccacacccg acagtacggt aaggcacccg tcaggcgggc ctgccacgcg gccgatcaca
     2461 ccgggagggt ggtcctcgat acactttacg atcaggctat gcggcagaac gtgaaattct
     2521 atgcggaata ttacgtcctc tcgctcgttt tcgacgaggg gcggtgtacg ggggtcgtga
     2581 cgtacaacct tgccaccggt gagttggacc ttctgcgggc gaaggcggtg atactcgcca
     2641 caggcgggtg cggcaagatc tatagaacga cgtctaacgg ctttgccacg accggcgaag
     2701 ccttgagcct cgtttaccgg gaaggcatcc cgctcgagga catggaattc gtgcagtttc
     2761 acccgacagg cctcttcccc ctcggtattc tcgtcagcga ggccgcccgg ggtgaagggg
     2821 ggatcctgcg caacgatgca ggggaaccct tcatggaacg ttacgcaccg gcgatcaaag
     2881 accttgcggc ccgcgacgtc gtatccaggg cgatattgac ggagatccgc gccgggcgtg
     2941 gcatcggggg aaaggcatac gtccacctcg acctcaccca cctgggagag cggaagatca
     3001 ccgagaaact gtgggagatc acttctttcg cacggat
//
LOCUS       DAOPOA010000217         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35188_length_3035_cov_3.374497, whole genome shotgun sequence.
ACCESSION   DAOPOA010000217 DAOPOA010000000
VERSION     DAOPOA010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35188_length_3035_cov_3.374497"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2598)
                     /locus_tag="PKJ17_05520"
     CDS             complement(<1..2598)
                     /locus_tag="PKJ17_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOD75464.1"
                     /translation="MSLNAASKEKPVQNFLTSLIEAYPDASMRDIAVLFTDVVGSTTF
                     FKTHGDIRGREMLRTHHHMAMSIVEEYGGSLIKEVGDSVLVYFPDPLEALKAAMKMQQ
                     RFLLHNRESSPQDEIHVRIGLHHGKVIVEEKDIYGDVVNVAAKLTNLAGGDQIFVSHE
                     VYESMRGEPPVQFERIDFWNMKNVPDGLSIYKVIWEVAQVPAPSRRVIMVLVPDPLHP
                     EISGIVDAADLCRGEKDDPPEDECVSINRSQGDKPVFTYATAPAAIEASKRVLSRLAE
                     RGGTPQDALLPVKIFITGVSAREEEEILANGPGTDLDPLPYGAIYVSPEVHQHMRSRY
                     RAHAGPAVTFEPVGRLFRISASEQVPPAAVPSRAEGTPAPSQGVHPPCFYCGGTHHKV
                     NKCPSRDLTDAGRSIQHLGYLPMQTIEAHGVSLERSLADMRVVPGPSSAGGALEGGEL
                     AALAFFDIRWVYQLRFLRVLWNNNAESWDSVRNAMTENEGGFAWLAQDSLRVSNYDKA
                     RSFLTLALERKPQDYKARCVAGLLNMELGDAPAAIADFRKALESAGTNVHKIYASLIL
                     ARLYKLSGDTVRFRHMLGEIGRLTGGCVEAAYEEILFKLEEKDEKGAMKKLVKLVRDD
                     PGFFVTVLIDPELAPYRNTIYAHLTELMAEAKNDAISHLEDARKELNRCRPLLPLSAL
                     KTIEPALQEIEMSIHSESYLGCLDVPRRCGSIRVMCKNAIEEQSRTITGTMKDLRARL
                     NNADAFLKTYRYSCLSEKHRGRLVYLRRSMERIGDVRYFESSVEFESCHQMCREISDE
                     LTSIEKRLEFLDLSANLIQMCLKFLKYSSILFSIVFFFGIFIFPLLSEEVNTVLSRMD
                     ISVFSSVWSL"
     gene            complement(2651..>3035)
                     /locus_tag="PKJ17_05525"
     CDS             complement(2651..>3035)
                     /locus_tag="PKJ17_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOD75465.1"
                     /translation="MERLGIEERDIVEKFVRSRGHGGQNVNKTSTCVYLKHVPSGIEV
                     KCQQERSQALNRFLARRILADKIEGIALGSESREQQLREKIRRQKRRRSRRAKEKVLH
                     LKHLTGEKKKARSFRPDRSDYPGDN"
BASE COUNT          576 a          880 c          916 g          663 t
ORIGIN      
        1 gagcgaccag acgctggaaa agaccgagat atccatccgt gagagcacgg tgttgacctc
       61 ttcggaaagg aggggaaata tgaatatccc gaaaaagaag acgatggaga agagaatgga
      121 ggaatacttg aggaacttga ggcacatttg gatgagattg gcggaaaggt ccaggaactc
      181 cagccttttc tcgatggagg tgagctcgtc cgatatctcc cggcacatct ggtggcaact
      241 ctcgaactcc accgaagact cgaaataacg cacgtcgcct atacgttcca tggaccgcct
      301 caaatagacc aggcgtcccc ggtgcttctc cgacaggcag gagtaccgat aggtcttcag
      361 gaaggcgtcg gcattgttaa gccgagcacg caggtccttc atcgtcccgg ttatcgtccg
      421 ggactgctct tcgatggcgt tcttgcacat cactctgatc gaaccgcacc ggcggggcac
      481 gtcgagacaa cccaggtaac tttccgagtg tatcgacatc tcgatctcct gcagggcggg
      541 ctcgatggtc ttcaatgccg acagaggcag gagggggcgg cagcggttga gctccttcct
      601 ggcgtcctca aggtgggaga tggcgtcgtt tttcgcttct gccatgagtt ctgtcaggtg
      661 cgcgtagatg gtgttcctgt aaggcgcgag ctcgggatcg ataaggacgg tcacgaagaa
      721 gccggggtcg tccctgacga gcttcacgag tttcttcatg gccccttttt catccttctc
      781 ctcaagcttg aaaaggatct cttcgtaggc ggcttcgacg cagccgccgg taaggcggcc
      841 tatctcgccg agcatgtgcc tgaacctcac ggtgtcccct gacagcttgt acagcctcgc
      901 tagaatgagg gaagcgtaga tcttatggac gttcgtcccc gccgactcca acgccttcct
      961 gaagtccgcg atcgccgccg gagcgtctcc aagctccata ttgagaagcc ccgctacaca
     1021 gcgggccttg taatcctgag gtttcctctc gagggcgagt gtgagaaagg acctggcttt
     1081 atcgtagttg gaaacgcgga gcgagtcctg cgccagccag gcaaaaccac cctcgttctc
     1141 cgtcatggca ttccggaccg aatcccagga ttccgcgtta ttgttccaga ggacgcgcag
     1201 gaaacggagc tgatagaccc accgaatgtc aaagaatgcc agcgccgcca gttcccctcc
     1261 ctcgagggcg ccgcccgccg atgaagggcc ggggaccacg cgcatatccg cgagcgatct
     1321 ctcgagggag acaccatgcg cctcgatcgt ctgcatggga aggtatccga ggtgctggat
     1381 cgacctgccg gcatccgtca ggtcccgtga tgggcacttg ttgaccttgt ggtgtgtgcc
     1441 cccgcaatag aaacaggggg ggtggacacc ctgggacggg gcgggggtgc cttcggcccg
     1501 cgacgggacg gccgcgggag ggacctgctc ggaggccgat atcctgaaga ggcggcccac
     1561 aggttcaaag gtgacggcgg gccctgcatg cgcacgatat cggcttcgca tgtgctgatg
     1621 cacctctggc gatacgtaga tcgcgccgta gggcaggggg tcaaggtcgg tgcccgggcc
     1681 gtttgccagg atctcctctt cctccctggc ggagaccccg gtaatgaaga tctttacggg
     1741 caggagggca tcctgcgggg tcccgcccct ttcagcgagc cgggatagca cccttttcga
     1801 tgcctcgatg gcggctggcg ccgtggcgta tgtgaagacg ggcttatcgc cctgcgacct
     1861 attaatcgaa acgcattcgt cttcaggggg atcgtccttc tcgccgcggc acaggtcggc
     1921 tgcatcgact atcccactga tctccggatg gagggggtcg gggacgagga ccatgatgac
     1981 cctcctcgac ggggcgggga cctgggcaac ctcccatatc accttgtata tgctgaggcc
     2041 gtcgggaacg ttcttcatgt tccagaaatc gatgcgttca aactgcaccg gaggctcgcc
     2101 cctcatcgat tcatacacct cgtgggagac gaatatctgg tctccgccgg caaggttcgt
     2161 cagtttggcg gcgacattga cgacatcgcc gtagatgtcc ttttcctcga ctatgacctt
     2221 tccgtggtgc aggccgatcc tcacgtggat ctcatcctgg gggctgctct cccggttgtg
     2281 gaggaggaat ctttgctgca tcttcatggc ggccttgagt gcttccaggg ggtcgggaaa
     2341 ataaacgagg accgagtcgc ccacttcctt tatgagcgac cctccgtatt cctcgactat
     2401 ggacatggcc atgtgatgat gggtcctcag catctccctg ccgcggatgt cgccatgggt
     2461 cttgaagaat gtcgtggagc ccacgacatc ggtgaacaat accgcgatat cccgcatgga
     2521 tgcgtccggg tacgcttcta tgagagatgt gaggaagttc tggacgggtt tctctttcga
     2581 ggcggcattc aggctcatga cgtacattgt ttatcggcag gggtgggaca aactttaacc
     2641 gggcgggctg tcagttgtcg ccgggatagt cggaacgatc gggccggaag gaacgggcct
     2701 ttttcttttc tcccgtaaga tgtttgaggt gtaggacctt ttccttggcc cttctcgagc
     2761 gtctcctttt ctgacgccgt atcttttcgc gcagctgctg ctccctgctc tcactgccca
     2821 gggcgatccc ttcgatctta tcagcaagga tgcgtctcgc gaggaaccgg ttcagggcct
     2881 gggagcgctc ctgctggcac ttgacctcga tccccgacgg cacgtgcttc agatagacgc
     2941 acgtcgaggt cttgttcacg ttctggccgc cgtggccccg ggaccgcacg aacttctcca
     3001 cgatgtccct ctcttctatg cccaaccgtt ccatc
//
LOCUS       DAOPOA010000218         3027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35300_length_3027_cov_3.855989, whole genome shotgun sequence.
ACCESSION   DAOPOA010000218 DAOPOA010000000
VERSION     DAOPOA010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35300_length_3027_cov_3.855989"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PKJ17_05530"
     CDS             complement(<1..508)
                     /locus_tag="PKJ17_05530"
                     /inference="COORDINATES: protein motif:HMM:NF022607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HOD75466.1"
                     /translation="MKKNEQVCIKWPILIGSACAALILLAVFAFLYARTTPHYSLYML
                     KKAIVNRDAGQVLRYLDTDSILDNMAKDVFARMDKKEPPRNKFEQHMRAAGKDVAAQL
                     LPQLKAQMGESITNLLLSYNDEQLFGNLRRATVFALTVTTHGNTADVRQRGKDRISFT
                     MAKSPEGHW"
     gene            complement(524..970)
                     /locus_tag="PKJ17_05535"
     CDS             complement(524..970)
                     /locus_tag="PKJ17_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75467.1"
                     /translation="MTGWLGNERGGAARWVFIIIIIAAAVFGYQYLKKTPRYALIQFK
                     RAILFSSAETAQRYAHFDSIVRSLPESETRGQSDETVRKRLLYEIDSPHEKTYFAKVK
                     GWSVMRCPITVTADQTSATAQPTENTSVTLQRLDNEQWVIVAIETQ"
     gene            complement(1007..2485)
                     /locus_tag="PKJ17_05540"
     CDS             complement(1007..2485)
                     /locus_tag="PKJ17_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOD75468.1"
                     /translation="MSDEQISNQENIEERSATESGHEEDFAQLFEQSTQSVKRLEPGQ
                     RVTTRVAGISGDFVYIDLGGKSEGVIDKKEFLNEEGAVTVQDGDEIEAYFVTVENGAR
                     KFTTLIHSFPSITLQSIKNAFDADIPVTGKVASELKGGYEIRVGKVRCFCPYSQMDLK
                     GSRDAGTYIGETFPFKVLEYKERGRNIILSRRALLEKELEKEKEKLKETLAAGMDIAG
                     KVRSIQRFGVFVDIGGIDGLIPLSELSWIKGSKPGDLVSIGDEVKTRILEIDWARDRI
                     TLSLKAVLPDPFLSVPEKYPVDSTVQGVVVRLESFGAFVNLEPGIDGLIPISKLGAGR
                     RINHPKEVLEPGQQVEARVVDISTEKRRLTLSMERKIVVEAVDLPAVGTLLETKVERV
                     ISSGIIVRIKEGLTGFIPNVEMGTLKGTNHSKMFPVGSPMQAVVVATDPSRGRVSLSR
                     TKVDEKIELDSYAQYRDKVREEERSDEKLGSLGELLRNKLGL"
     gene            2662..>3027
                     /locus_tag="PKJ17_05545"
     CDS             2662..>3027
                     /locus_tag="PKJ17_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HOD75469.1"
                     /translation="MNFVSLKRSCVLAFVAMFLMLSVTTAVPVLAQSKGGPALTREKT
                     GYALGVSIGRNMKNLGIDIDPDMIVRGIRDTVRGQLSMSDQEVKSTLDEYEKDLIKRL
                     GDKNKREGEAFLKENQQRKS"
BASE COUNT          574 a          823 c          877 g          753 t
ORIGIN      
        1 gccagtggcc ttcgggggat ttggccattg tgaagcttat cctgtccttg ccgcgctggc
       61 ggacatcggc ggtgttgccg tgggttgtga cggtcagagc aaagacggtg gccctgcgga
      121 ggttgccgaa gagctgctcg tcgttgtagg aaaggagcag gttcgttatg gattcgccca
      181 tctgggcctt gagctggggc agcaactggg cggcgacgtc cttccccgcg gctcgcatgt
      241 gctgctcgaa cttgttgcgc gggggttcct tcttgtccat gcgcgcgaac acgtcctttg
      301 ccatgttatc gagaatgctg tcggtgtcga ggtaccggag gacttggccg gcgtcgcggt
      361 tcactatggc tttcttcagc atgtacaggc tgtagtgggg agtggtcctg gcgtacagaa
      421 aggcaaagac ggcgaggagt atgagggcgg cacaggcgga gccgataagg atcggccact
      481 ttatgcatac ctgttcattc tttttcatga ggcgcccgga tgcctattgt gtctcgatcg
      541 cgactatgac ccattgctcg ttgtccagtc tctggagggt aacggaagtg ttttccgtcg
      601 gctgtgccgt cgctgaggtc tgatcggccg tgacggtgat agggcaccgc atgacggacc
      661 accccttcac cttcgcgaaa taggttttct cgtgggggga gtctatctca tagaggaggc
      721 gtttccttac cgtttcgtca gactgcccac gggtctcgct ttcgggcaat gaccggacga
      781 tgctgtcgaa atgggcgtat ctctgggccg tttcggcact gctgaagagg atggccctct
      841 tgaattggat cagggcatac cggggggtct tcttgaggta ctggtagccg aatacggcgg
      901 ccgcgatgat gatgataatg aagacccatc gcgctgcccc tccccgttca tttccgagcc
      961 agcctgtcat cgatctggcg acctcctccc ctcagggtga tcgtcgttac agtccgagtt
     1021 tgttcctcaa aagctcaccg aggctgccca atttctcatc ggaccgctct tcttccctca
     1081 ccttgtccct gtactgggcg tagctgtcga gctctatttt ctcatcgacc ttggtcctgg
     1141 acagactgac gcggccgcgg gaggggtcgg tggcgaccac caccgcctgc atgggagatc
     1201 ccacggggaa catcttgctg tggttggtgc ctttcagggt acccatctcg acgttgggaa
     1261 taaagcccgt caatccttcc ttgatccgta cgatgatgcc cgatgagatg acacgttcca
     1321 ccttcgtctc gaggagtgtg ccgaccgcgg gcaggtccac ggcttccaca acgatcttgc
     1381 gctccattga gagggtgagt cttctctttt cagtgctgat atccacgacc ctggcctcga
     1441 cctgctggcc gggttcgagc acttccttgg gatggttgat ccttctgcct gccccgagct
     1501 ttgaaatggg gatgagcccg tctatgccgg gttcgaggtt gacgaaggcg ccgaaactct
     1561 caagacggac aaccacccct tggaccgtgg agtccacggg gtatttctca ggaaccgaca
     1621 ggaagggatc gggaaggacc gccttgagac tcaatgttat cctgtccctt gcccagtcga
     1681 tctcgagtat cctcgtcttc acctcgtcgc ctatactgac gaggtctccc ggtttcgatc
     1741 ctttgatcca tgagagttcg ctcagtggaa tgagaccgtc gatgccaccg atgtcgacaa
     1801 agacgccgaa tctctggatc gaccgcactt tgcccgcgat gtccatccct gcggcgaggg
     1861 tctccttgag cttttccttc tccttttcca gttccttctc cagcagggca cggcgggaaa
     1921 gaatgatatt ccggccccgt tccttatact caagcacctt gaagggaaat gtctcgccga
     1981 tgtaggttcc ggcatccctg ctccccttga ggtccatctg cgagtacggg cagaaacagc
     2041 gcaccttgcc gacgcggatc tcgtagccgc ccttgagttc cgacgccacc tttcccgtga
     2101 cggggatgtc ggcgtcgaag gcgttcttga tgctctggag ggtgatgctc gggaagctgt
     2161 ggatcaaggt ggtgaacttt ctcgctccat tttcaacggt cacgaagtag gcctcgatct
     2221 cgtcgccatc ctgaacggtg accgcgcctt cctcgttgag aaactctttc ttgtcgatga
     2281 cgccctcgct cttgccgccg aggtcgatat agacaaaatc gcccgaaatg ccggcgaccc
     2341 gtgtggtcac cctctgtccc ggttcgagtc tcttgaccga ctgcgtgctc tgttcgaaaa
     2401 gctgtgcaaa atcttcttcg tgtccgcttt ctgttgcgct cctttcttca atattttcct
     2461 ggttgctgat ctgttcgtct gacatcgtcc gatctcctga agttgttaaa aaattttttg
     2521 ttccagtaat gtacaatttc tgaagtgtta tgtaaagcta tagttctcat taatcgggaa
     2581 tttgacaatt ctaaggccat ggaataacat atgaaacatg taaaaaccca caggccgtgt
     2641 gcggacggaa aggaggaagg ggtgaatttt gtttccctga agagatcctg cgttttggcg
     2701 tttgtcgcga tgtttctgat gctctctgtg acgactgcgg tgcccgttct ggcgcaaagc
     2761 aagggcggac cggcacttac gagggaaaag accggatacg cgcttggcgt gagcatcggt
     2821 cgcaacatga agaatcttgg aatagatatc gatcctgaca tgatcgttcg cgggatacgg
     2881 gataccgttc gggggcagct ttccatgtcg gatcaggaag tgaagtcgac cctcgacgaa
     2941 tacgagaagg acctcatcaa gaggctgggc gacaagaaca aacgggaagg ggaggcattc
     3001 ctcaaggaga accagcagcg taagagc
//
LOCUS       DAOPOA010000219         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35316_length_3026_cov_2.973746, whole genome shotgun sequence.
ACCESSION   DAOPOA010000219 DAOPOA010000000
VERSION     DAOPOA010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35316_length_3026_cov_2.973746"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PKJ17_05550"
     CDS             complement(<1..289)
                     /locus_tag="PKJ17_05550"
                     /inference="COORDINATES: protein motif:HMM:NF023853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOD75470.1"
                     /translation="MELTEIFGSNVFNDKVMKQRLPKEVYKALRKTIQQGTPLRPDVA
                     DVVANAMKDWAIEKGATHYTHWFQPLTGITAEKHDSFISPAPDGSVIMEFSG"
     gene            complement(497..1744)
                     /locus_tag="PKJ17_05555"
     CDS             complement(497..1744)
                     /locus_tag="PKJ17_05555"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOD75471.1"
                     /translation="MIIFMTGYHLPSRPGNAIAPARIAVILVTILLTLVVAARPAVAL
                     EVGPVPQAVQDRFLPEGDNIVVRPWISGLEVPWSLVFLPDGRALVSERPGRIRLIRKG
                     VLAPRPWATIDVRSAGEGGLMGLAVHPAFPGEPYVYAMYTYTEKGETMNRVVRFKDDG
                     DTGRFDRVILDRIPGARNHNGGRIAFGPDGMLYICTGEKYERKLAQDLNSLNGKILRV
                     TPGGVIPPDNPFNSPVWSYGHRNPQGLAWHPVTGELFVSEHGPSGESLVFGNDEINII
                     VRGGNYGWPVVAGAPSVKPYRDPIIVWKRATPPGGITFCRKGPLSHLENDLFIATLRS
                     DALIRVKLRRERDRYAVTAIERWFVAGDVEGRYGRIRDVVQGPDGYLYFLTSNRDGRG
                     SPRQDDDRIYRIMPRQDVSYRNR"
     gene            complement(1982..>3026)
                     /locus_tag="PKJ17_05560"
     CDS             complement(1982..>3026)
                     /locus_tag="PKJ17_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD75472.1"
                     /translation="FEALGPEARHLEEEIQRAMARKEIPEGHTYLITPKNLQEYLRDD
                     PGTTLPEIITTKTHSVLPGQYLEGGRKSVITRSAGYDHFEHLADRVNIASLREYCVNA
                     VAQTAIKFLYAAAGELNHYSLNTVTFERKDSRAFMELDRHRVLTVFGVGKIGKRTYEL
                     AEANGLTVQGVDVRREELDRLYGGSVRFVSKDEAIRSSDIIVNAMNLTRNPESRFYNM
                     GYFSKEYLSQAKKNLIFINVTRGEIAPESVLLDLYRSGRITGIGLDVFSNESQFSALL
                     LGQPAETDANLLAARALVEKSIDRSSNIYVQPHQGFNSDIAALTKAVEAVKHVAAWYR
                     NGKERFDEQLPYY"
BASE COUNT          566 a          863 c          900 g          697 t
ORIGIN      
        1 tgccggaaaa ctccatgatg acgctgccgt cgggggcagg ggagataaaa gagtcgtgct
       61 tctcggcggt gatgcccgtc aagggctgga accagtgcgt atagtgcgtc gcgccctttt
      121 cgatggccca gtccttcatc gcgttggcca ccacgtcggc cacatcgggc cgaaggggcg
      181 tgccctgctg gatcgtcttc cggagggcct tatagacctc cttcggcagg cgttgtttca
      241 tcaccttatc gttgaataca ttggatccaa atatctctgt aagttccatt ttaatcctcc
      301 tcacaagttg gaccggacaa agccgtataa taacactttc cacctgtctc tggaaaggtt
      361 tgttcagtcc gtaaacattg actttgggtg aacttctttg ttcggtatgc ttgcttagcc
      421 ctcggggccg ttgaggggaa cacatcaatg tgtgtgaaca gtataatatg atgctgttca
      481 acttacaagt aaattgtcag cggttccggt aagacacgtc ctgccgtggc atgatccgat
      541 agatcctgtc gtcgtcctgc cgcggactgc cccgcccgtc cctgttgctt gtcaggaagt
      601 acagatagcc gtcgggtccc tggacaacgt cgcggatgcg tccgtaacgt ccttccacgt
      661 ctcctgcgac gaaccatcgt tcgatggccg tgacggcata gcggtccctc tctcttctca
      721 acttcacgcg gatcagggcg tcgctcctca aggtcgcgat gaagaggtcg ttctcgagat
      781 gggaaagagg ccccttcctg cagaacgtga tgcctcccgg cggggttgcc ctcttccaga
      841 cgatgatagg gtccctgtat ggtttgaccg atggggcgcc ggcaaccacc ggccatccgt
      901 agttgccgcc cctgacgatg atgttgatct cgtcgttgcc gaaaaccagg gattcacccg
      961 aaggtccgtg ctcagacacg aaaagctccc ccgtgacggg gtgccaggca agaccctgag
     1021 gattgcgatg cccgtaggac cagaccggcg agttaaaggg attgtcggga gggatgacgc
     1081 ccccgggggt gacccgcagg atcttcccgt tgagggaatt gaggtcctgg gccagttttc
     1141 tttcgtattt ttcgcccgta cagatgtaga gcatgccgtc ggggccgaag gcaatccttc
     1201 cgccgttatg gttcctggcc cccggtatcc tgtccaggat gaccctgtcg aagcgccccg
     1261 tgtccccgtc gtctttgaag cggaccaccc tgttcatcgt ctcgcccttc tccgtgtagg
     1321 tgtacatggc atacacgtaa ggttccccgg gaaaggccgg atggacggca aggcccatca
     1381 ggccgccttc gccggcgctc cggacatcga tcgtcgccca ggggcggggc gcaaggacac
     1441 ctttcctgat gaggcgtatc ctgcccggtc tctcgctgac gagtgccctg ccgtcgggga
     1501 ggaaaacgag cgaccagggc acctcgaggc ccgagatcca cggcctgaca acgatgttgt
     1561 ccccctcggg gaggaacctg tcctgcaccg cctgggggac gggacccact tccagggcaa
     1621 ctgccggtcg tgccgccaca acaagcgtga ggaggatcgt gaccaggatg acggcaatac
     1681 gtgccggcgc tattgcattc ccgggccgtg atggaaggtg gtagcctgtc atgaagataa
     1741 tcatatggac caccggtcga tgtgtcaacc ggtcgcatcg gtagcgatcc gtcgagactt
     1801 gtcttacctc tcttccggca gggtgggtca tgggaagttt tgcgctggaa ccccgtcagg
     1861 aggcgactcc atcaactact agaaaaaccg ggactgttta gaacaatagt acttaataac
     1921 gttatgaaat gtcacgaaag tgattctata cggtcgatgc ttgcgactat tttccccggc
     1981 atcaataata ggggagttgt tcgtcgaaac gctccttgcc gttcctgtac cacgcggcaa
     2041 cgtgcttgac cgcttcgacg gccttcgtca gggccgcgat gtcggaattg aagccctggt
     2101 ggggctggac gtagatgttg gaactcctgt cgatggactt ctcaacgagt gctctggccg
     2161 caagcaggtt ggcgtcggtc tccgccggct gtccgaggag cagggccgag aactgcgact
     2221 cgttgctgaa gacgtcaaga ccgatccccg ttatccttcc cgagcgatag aggtcgagaa
     2281 gcaccgattc gggggcgatc tccccgcggg tcacattgat gaagatgagg ttcttcttcg
     2341 cctgggagag atattccttc gagaagtatc ccatgttgta gaacctgctc tccggattcc
     2401 tcgtcaggtt catggcattg acgatgatat cgctggaccg gatggcttca tccttcgaaa
     2461 cgaaccggac cgagcctccg taaagacggt cgagttcctc ccgcctgacg tcgactccct
     2521 gtacggtcag gccgttggct tcggcaagtt cgtatgtcct cttgcctatc ttgccgacgc
     2581 cgaacaccgt caggaccctg tgcctgtcga gttccatgaa ggcccgggaa tccttcctct
     2641 cgaaggtgac ggtgttgagg gagtagtggt tcagttcgcc ggcggcggcg tagaggaact
     2701 ttatggccgt ctgggcaacg gcgttcacgc aatactctct cagcgaggcg atattgaccc
     2761 tgtcggcgag gtgttcgaaa tggtcgtacc cggcgctgcg ggttatcacg ctttttcggc
     2821 ccccttcgag atactgtccg ggaagaacgg aatgcgtttt tgtggtgatg atctccggga
     2881 gggtcgtccc ggggtcgtcc ctgaggtatt cctggaggtt cttcggggtt atgagatatg
     2941 tgtgcccttc ggggatctct ttccgggcca tcgccctctg tatctcctcc tcgagatgcc
     3001 gggcctcggg ccccagggct tcgaaa
//
LOCUS       DAOPOA010000220         3022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35381_length_3022_cov_3.616785, whole genome shotgun sequence.
ACCESSION   DAOPOA010000220 DAOPOA010000000
VERSION     DAOPOA010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35381_length_3022_cov_3.616785"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PKJ17_05565"
     CDS             <1..1003
                     /locus_tag="PKJ17_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD75473.1"
                     /translation="LASLTRVDYDRDRFEACVIADNCTDGTAGIAAQHNARVLERHDA
                     SRKGKGFAIEWAMKQVDLGAFDAVVIADADNLIDPGFFRGLDEVLDGGRRAVQCNNCL
                     ANSRDTVFTGVIHLSRTINNELYHHVKHRIGLSSYLMGNGMCFTTRLLKEHPWMTGTM
                     AEDYEYYAKLVASGEIVGFAANSRLYHQESRGLRHASGQRLRWSAGRFEVAKRYGLRL
                     FMLGLKERSYRIVDASFPLILPNLSLMVNMTAAALAAGLVTHYFIPAPFVVLWLALLM
                     AMEVAYFVSGVYLTGMPVWRFLHAIGFAPLFLVWKGCIDLLGISGKRTKQWGRSGRL"
     gene            1042..1839
                     /locus_tag="PKJ17_05570"
     CDS             1042..1839
                     /locus_tag="PKJ17_05570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD75474.1"
                     /translation="MISKTQAVAVDDHLHRVKDFHDADASRYRADRYHSETCEGLAYV
                     TRRQIVLAMADVEPGRVLDVGCGPGILTRDLLKKGHEVYSADLSIGMIREARRAACAA
                     EGSVLPHFIVSDASRICVSTDRMDMVLSIGLMCYVKDHRGVLSEILRVLRPGGVAVIQ
                     VNNIRWPGLYRAFVPLYHRLKTLIGAKRYDGLDFDFNFSSRKKFLDDLVEIGFHVLDI
                     ERYDFRVPFLDILLPRLSVTMGKLMFRGRHRWVYRCFSHGLLIKVRK"
     gene            complement(2002..2841)
                     /locus_tag="PKJ17_05575"
     CDS             complement(2002..2841)
                     /locus_tag="PKJ17_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75475.1"
                     /translation="MKKIITGMAILLLLAICHTGHATVIWQESFDNFTRDYNCSLSDC
                     SSNPGVPTGYMSFGSNTLGKNYGNPNTQIVGGAERSNPGATNKRGFRINLEEQCWPTG
                     ENILVKDLGRNYTLLYLRWYQRDSTKSFSNFQKLFRLKQSNGQILIPEFQVNGSNIQM
                     NLWDAQSSSNHFFTGYSLNSHYTANTWVCYEIKIDLVNKKAEFWVNGVSKGTLNATWW
                     PDGWYIRYVEIGGNQYGHSWTAPTEHTRDYDDIVVADSYVGPTGAPASEVPAPKNLRI
                     LSQ"
BASE COUNT          558 a          830 c          944 g          690 t
ORIGIN      
        1 cctcgcgagc ctcacccggg tggactacga ccgggatcgt tttgaggcct gcgtcatcgc
       61 ggataactgc accgacggga cagccgggat tgccgcacag cacaatgcca gggtgctcga
      121 gagacacgat gcttcaagga agggcaaggg ttttgccatt gagtgggcca tgaagcaggt
      181 cgacctcggt gctttcgacg cggttgtcat cgccgacgcg gacaacctca tcgaccccgg
      241 gttcttccgc ggcctcgatg aggtcctgga cgggggaaga cgcgccgtcc agtgcaacaa
      301 ctgccttgcc aattcccgcg acacggtttt caccggggtc atccaccttt cgaggacgat
      361 caacaacgag ctctatcatc atgtcaagca ccggatcggc ctgtcgtcgt atctcatggg
      421 gaacggtatg tgcttcacga cgcggctcct caaggagcat ccctggatga cggggacgat
      481 ggccgaggat tacgagtact acgcgaaact tgtggcaagc ggcgagatcg tcgggtttgc
      541 ggcaaattcc cggctctatc accaggaatc gagggggttg aggcatgcct cgggacagcg
      601 gctccgctgg tcggcgggac gcttcgaggt ggcgaaacga tacggcctca ggctcttcat
      661 gctaggcttg aaggagagaa gctacaggat cgtggacgcc tctttccccc tcattctgcc
      721 aaacctgtcg ctcatggtga acatgaccgc cgccgctctg gcggcgggcc ttgtcacgca
      781 ttatttcatt cccgctccct ttgttgtgct ttggctcgcc cttctcatgg cgatggaggt
      841 ggcttacttc gtgagcgggg tctacctcac ggggatgccg gtctggaggt ttctccatgc
      901 catcggtttc gcgcccctct tccttgtgtg gaagggctgc atagacctcc tgggaatatc
      961 gggaaagagg acgaagcaat ggggaaggtc gggaaggctg tgagaccggc gggtccgccg
     1021 gtcctgaatg aggaggcgag aatgatatcg aagacgcagg cggtcgccgt ggacgaccac
     1081 ctccaccgtg tgaaggattt ccatgacgcc gatgcctcac ggtatcgcgc cgaccggtac
     1141 cactcggaga cctgtgaagg gcttgcgtat gtcacgcgca gacagatcgt cctcgcgatg
     1201 gccgacgttg agcctggccg ggtcctcgat gtcggctgtg ggccggggat cctcacccgc
     1261 gacctgctga agaaagggca cgaggtctac agcgcggacc tgtcgatagg gatgatccgg
     1321 gaggcgagac gcgccgcgtg tgcggcagag ggttccgtgt tgccgcactt cattgttagc
     1381 gatgcctcga ggatctgcgt ttcaacggat cgcatggata tggtcctctc catcgggttg
     1441 atgtgctacg tgaaggacca caggggcgtg ctctcggaga tcctgagggt cctcaggccc
     1501 ggcggtgtcg ccgtcatcca ggtcaacaac atacgatggc cgggactgta ccgcgcgttc
     1561 gtccccctct atcaccggct gaagaccctt atcggcgcga agagatatga cgggctggat
     1621 ttcgatttca atttctcttc gcggaagaag ttcctcgatg atcttgtgga aatcgggttc
     1681 catgtgctcg atatcgagcg ctacgatttc agggttccct ttttggacat tctcctgcca
     1741 aggctatccg tcacgatggg aaagctcatg ttcaggggca ggcacaggtg ggtgtacaga
     1801 tgcttttccc acggtcttct gataaaggtg agaaaatgat cactcctttt ggccactgcg
     1861 ggacggcccg ggagggtcgg gccggacagg cgctgggtat cgaggggaac ggtcgggaac
     1921 ggaaaagatc ggggccgcac cgggccggag gaaaggcgtc agtggtgcag ccccgatccc
     1981 tttcggggtg gaaagaccgt gttactggct cagtatcctg aggtttttcg gagcgggtac
     2041 ctccgacgcg ggcgctcccg tgggaccgac gtaggaatcg gccacgacga tatcgtcata
     2101 gtcgcgcgtg tgctccgtgg gcgccgtcca cgaatgcccg tactggttgc cgccgatctc
     2161 gacgtaccgt atgtaccagc cgtcgggcca ccaggtggcg ttcagcgttc ctttggagac
     2221 gccgttgacc cagaattcgg ccttcttgtt aacgaggtcg atcttgatct cgtagcagac
     2281 ccacgtgttg gcggtgtaat ggctgttgag actgtacccg gtgaaaaaat ggttactgga
     2341 gctctgggcg tcccagaggt tcatctgaat attgcttccg ttcacctgga attcggggat
     2401 gaggatctgg ccattcgact gtttcagccg gaacagtttc tgaaaattgc tgaatgattt
     2461 tgtggaatct ctctggtacc agcgcaggta gaggagggtg tagttcctgc caaggtcttt
     2521 tacgagtatg ttttcccccg tgggccagca ctgctcttcc aggttgatgc ggaagcccct
     2581 cttgttcgtt gcgccggggt tcgacctttc ggcgcctccg acgatctgag tgttggggtt
     2641 tccgtagttc ttgccgagcg tattggaacc aaagctcatg tatcccgtgg gcacgcccgg
     2701 gttggaagag caatccgaca ggctgcagtt gtaatccctg gtgaagttgt caaaactttc
     2761 ctgccagatg accgtcgcat gacctgtgtg acatatagcg agcagtaaca atatcgccat
     2821 tcctgtgatg atctttttca cttgtttgcc tccttgcttt ctctcggtgc tgcttgtgcg
     2881 gtccggtgat gttccgtccg gttgtcccgc gggcgccccg ggaaggatcc cgcatgcacc
     2941 cgcgaggacc gtacttctat tatagtccat ggtggtcgac atccccacgc gaatatttca
     3001 tgggctccgt ctcaatagta ac
//
LOCUS       DAOPOA010000221         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35428_length_3019_cov_3.136302, whole genome shotgun sequence.
ACCESSION   DAOPOA010000221 DAOPOA010000000
VERSION     DAOPOA010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35428_length_3019_cov_3.136302"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PKJ17_05580"
     CDS             complement(<1..183)
                     /locus_tag="PKJ17_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75476.1"
                     /translation="MGVYSYKATTLEGAIIEGTIEAPDENLALERIKDWGAIPLKVSS
                     PGKGVLKKGVSLHSSKG"
     gene            complement(184..1716)
                     /gene="gspE"
                     /locus_tag="PKJ17_05585"
     CDS             complement(184..1716)
                     /gene="gspE"
                     /locus_tag="PKJ17_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOD75477.1"
                     /translation="MNGKTIIHLSPEEFPRVPLVMDGIATRFIRENKIIPFEWKDGTL
                     KIIMADPSKRELVDALRAATASDVLVHSGDPAVIDEHISRFYTKDQQNLDKIIEGIDE
                     REGYEFIRDEEEEDVGHLKDLASEAPIIRLVNLFITRAVELRASDIHIEPFEDEMKIR
                     YRIDGVLNDVESTPKRLQAAIVSRIKIMARLNIAERRLPQDGRIRLRVGEKEIAIRVS
                     TIPIVNGESVVMRLLDRESIVIDLDKLGFSQAMLMSLDRLITKPNGIILVTGPTGSGK
                     TTTLYGALDKINSPEKKIITVEDPVEYQLKGVNQIQVKPQIGLDFNSTLRHIVRQDPD
                     IIMIGEIRDLETAQIAIQSALTGHLVFSTLHTNDAPSAITRLLDMGVESFLLSSTVRG
                     ILAQRLVRMICPDCRRQTGVSDDVEELMALGMGGGVPVWRGAGCEACGHTGFFGRSGL
                     FELLVVDEDLRRLTLKGADEGEIRAQARRSGMKTLLEDGMEKIRMSVTTLDEVFRVTR
                     ED"
     gene            complement(1713..>3019)
                     /locus_tag="PKJ17_05590"
     CDS             complement(1713..>3019)
                     /locus_tag="PKJ17_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOD75478.1"
                     /translation="VYHVQNSKAKDIANILQQVFLSSGRGGAANPPLAALPSAGKTAG
                     ASGTPAARSGPEGGLIVSGSQAGEGLISPVTKIIAEENLNSLIILSLPEDYEVIKEAI
                     RRVDIIPRQVIIEGVIAEITLKDDLKLGVSWALQFSPDGMNGVLGAVDGTVGFDVPGA
                     TATSTTGSGTFTFAGTVGGDFKAVIDTLATQSKAKLLAVPRILVTDNKEARIQVGEQV
                     PIVTTETIASTTTTAQRTIQYKDIGIILKVKPRINEGGLVTLDLAQEVSSYDTITLFD
                     GETNIIVKKTEATTNLVVQDGQTILIGGLIREDISAARSGIPYLSKIPLLGYLFGSTT
                     DENHRKELIILLTPRVIKNQHDARKATSSYVDDITKTGKGKLKKEELIRDVPRQEKDG
                     KNDAGETREGRGTKEHEATHTEVPSAGEEPAAKAAAGSTGES"
BASE COUNT          524 a          932 c          847 g          716 t
ORIGIN      
        1 gcccttcgat gagtgcagcg acacgccctt tttgaggacc ccctttcccg gtgacgaaac
       61 cttcaaaggg atggcgcccc agtccttgat cctctccaag gccaggtttt catcgggcgc
      121 ctcgatggtg ccttcgatga ttgcgccttc aagggttgtc gccttgtatg aatacactcc
      181 caatcaatcc tccctcgtca cccgaaagac ctcgtccagg gtggtcacac tcatgcggat
      241 cttctccatc ccgtcctcga gaagcgtctt catgcccgac cttctcgcct gcgccctgat
      301 ctcgccctcg tcggcaccct tcaaggtcag ccgcctcaga tcttcatcga caacgagaag
      361 ctcgaagagc cccgagcgcc cgaagaaccc cgtatgtccg caggcctcgc agcctgctcc
      421 ccgccagacg ggcacgccgc cgcccatgcc gagggccatg agttcctcca cgtcgtcgga
      481 tacacctgtc tggcgcctgc aatcggggca gatcatcctc acgagcctct gcgcgaggat
      541 gcccctgacc gtggatgaca ggagaaagct ctccacaccc atgtcaagaa ggcgtgttat
      601 tgcgctcggc gcgtcgttgg tgtggagcgt cgagaatacg agatgccccg tcagtgccga
      661 ctggatggct atctgagcag tctccaggtc ccggatctcg cctatcatga taatgtccgg
      721 gtcctgcctg acgatgtggc gcaacgtgct gttaaaatcg agcccgatct ggggcttgac
      781 ctggatctgg ttgacacctt tcagctggta ctccacgggg tcctccacgg tgatgatctt
      841 cttttccggc gagtttatct tgtcgagtgc cccgtagagg gtcgttgtct taccgctgcc
      901 cgtcggcccc gtcacaagga tgatgccgtt aggtttcgtg atgagcctat cgagggacat
      961 gagcatggcc tgtgaaaaac ccaatttgtc aaggtcaatg acgatgcttt ccctgtcgag
     1021 cagcctcatg acgacgctct ctccgttcac gatggggatg gtggaaactc tgatggcgat
     1081 ctccttctcg cccacgcgca gccgtatcct tccatcctgg ggaagcctgc gctcggcaat
     1141 gttgagcctg gccatgatct ttatgcgcga gacgatggcc gcctgaagtc tcttcggtgt
     1201 cgattccacg tcattgagga cgccgtctat gcggtagcgg atcttcatct cgtcttcgaa
     1261 aggctcgata tggatgtcgc tcgcccttaa ctccacggca cgggtgatga agaggttcac
     1321 gagcctgatg atgggtgcct ccgacgcaag gtccttgagg tggcctacgt cctcctcttc
     1381 ctcatcacgt atgaactcgt atccctcccg ttcgtcgatt ccctctatga tcttatcgag
     1441 gttctgctgg tccttcgtgt agaaccggga gatgtgctcg tcgatgacgg cggggtcccc
     1501 gctatggacc aggacgtcgc ttgcggtggc cgccctcagc gcgtcgacaa gctcccgctt
     1561 ggaaggatcg gccatgatga tcttgagcgt accatccttc cactcaaagg ggatgatctt
     1621 gttctccctg atgaaacgcg tcgctatgcc gtccatgaca agaggcaccc tgggaaactc
     1681 ctccggggag agatgaatta tcgtcttgcc gttcacgact ctcctgtgct ccccgccgcc
     1741 gccttcgccg ctggttcctc tccggccgac gggacctctg tgtgcgtcgc ctcgtgttcc
     1801 tttgttcccc gcccttccct ggtttcgccg gcatcgttct tcccgtcctt ctcctgccgg
     1861 gggacgtcgc ggataagctc ttccttcttc agcttgccct tgcccgtctt cgttatatcg
     1921 tcaacatagg aggacgtggc cttccgggcg tcgtgctggt tcttgatgac ccgcggggtg
     1981 agaagtatga tcagctcctt acggtgattt tcgtccgtgg tactgccgaa gagatatccg
     2041 agaagcggta tcttgctgag gtacggaatg cccgatcgtg ccgcagatat gtcctccctg
     2101 ataagccctc cgatgaggat cgtctggccg tcctgaacga caaggttcgt cgtcgcctcc
     2161 gtctttttca caatgatgtt ggtttcgccg tcgaaaaggg tgatagtgtc gtaggaggag
     2221 acctcctggg caaggtcaag ggtaacaagc ccgccttcgt tgatacgggg ttttaccttg
     2281 aggatgatac cgatgtcctt gtactggatg gtccgctgag cggtggtggt ggtggatgct
     2341 atcgtctccg ttgtcacgat ggggacctgt tcacccacct ggatccgagc ctccttgtta
     2401 tccgtcacca gtatgcgcgg tacggcaaga agcttcgcct ttgattgcgt cgcgagcgta
     2461 tcgatgaccg ccttgaagtc acctccgacg gttcccgcaa aggtgaatgt cccgctgcct
     2521 gttgtgctcg tcgctgtcgc gccgggcaca tcgaatccga cggtgccgtc caccgccccc
     2581 agcaccccgt tcataccgtc cggcgagaac tgaagggccc aggaaacgcc aagcttcagg
     2641 tcatccttca gggtgatctc cgcgatcacc ccttcgataa tgacctgcct cgggataata
     2701 tcgacgcgcc ggatggcctc cttgatgacc tcgtaatcct cgggcaggct aaggataatg
     2761 agggagttga ggttctcctc ggcgatgatc ttcgttacgg gagaaataag gccctctccc
     2821 gcctgggagc ccgacacgat caagccgccc tccggcccgc tgcgcgcggc cggggtgccg
     2881 gatgcgcctg ccgtcttccc cgcggaaggc aacgcagcga ggggtggatt tgctgcgccg
     2941 cctcggccgg aggaaagaaa aacctgctgc agaatgttcg ctatgtcttt agctttgctg
     3001 ttctggacat gatagacaa
//
LOCUS       DAOPOA010000222         3009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35591_length_3009_cov_3.664523, whole genome shotgun sequence.
ACCESSION   DAOPOA010000222 DAOPOA010000000
VERSION     DAOPOA010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35591_length_3009_cov_3.664523"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PKJ17_05595"
     CDS             complement(<1..575)
                     /locus_tag="PKJ17_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75479.1"
                     /translation="MMKVLRSIPRNVTLLNVLLAVALVLSGIGVVCPAMSVSDRLKLP
                     RIVPKETVLPERPAEEAAQPLPTDYAVVSEMNLFHPQRVIPIDAKKELPKPELLLCGI
                     IMHGNQRVAFVEDKKNPTTSPGRGKRQTELRKGSVYAGYSVTEINTDSIALSRGEQTV
                     SVYLMDQAKRGGKSATSAPSEARPSSTERSSS"
     gene            complement(577..1131)
                     /gene="gspM"
                     /locus_tag="PKJ17_05600"
     CDS             complement(577..1131)
                     /gene="gspM"
                     /locus_tag="PKJ17_05600"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOD75480.1"
                     /translation="MKKENRILIYAIPATIVLAALTVYQYGLVNARKEMVSLRETRAV
                     RERLLMKCMETIAGRPRIEKKLDALKEKRGAAASKTIEAQTPTLCAAALQEAVKGIIT
                     GRGGSITSERVEKTEESGRFQVVTITVDAALPDTRALSDVLFGIETHTPYIVVREMDT
                     RVRNFRDPRELMVKLRLSSLAGGK"
     gene            complement(1115..2659)
                     /locus_tag="PKJ17_05605"
     CDS             complement(1115..2659)
                     /locus_tag="PKJ17_05605"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOD75481.1"
                     /translation="MALSPAIEKGIAYVRTVREKVEPLCLRIWAALNCNLVEGIILPE
                     RFLAVCMERGCVSAAAGERFLNRRRLIGSGRYALEGDGFPAPDDLAAAASRAMKELGR
                     RGSKVLLGVPREWVVIRTADMPSTVKENMGPVVTYEIDRLTPLTAADAMYDHVVGAER
                     DERIAITLMAARKERLNPCVAALRKKGLPPVKVSTPSVEMARLCGRLAGSRTFVSLHV
                     DPYGYEGWSVEEDNLAGNFYGSFDSCGDEEDPQRRYEDVRAALAAAGAADGSRPVYLV
                     AENGVGAGDVLPDIAHRQIGDSETMSLFGGSQKNVPLSLAAMLHEALFPGLATFDLAR
                     GGAGQKKKGHLVASLVLAALLVMMLIPYVVVPLEIEKRRIAAIEGQIRSLRKDVMEVE
                     RLKKEAEALQGELDHMEEFKKKRPMSLNILKGLTLLLPKTVWLTRARVTEETVEIEGY
                     AASATGVLSILEQSDLFRKVEFASPTMKDARLNGERFVLRMELEGFKKEEAKTEGTVK
                     KDEKGK"
     gene            complement(2671..>3009)
                     /locus_tag="PKJ17_05610"
     CDS             complement(2671..>3009)
                     /locus_tag="PKJ17_05610"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOD75482.1"
                     /translation="VYGDASKINVNFAAKEVIMALPGVSEDRAARIIDRRKQAELKTI
                     DDVRGIMGGEYGNVSSYIDVAESVGYAIESTGYREGAGEGHGIRATLLVRTGGTYEYI
                     YYKIPAETTK"
BASE COUNT          559 a          963 c          789 g          698 t
ORIGIN      
        1 gaagatgacc gctctgtgga agatggtctc gcctcggagg gagcggacgt ggcgctcttg
       61 ccgccgcgtt tcgcctggtc cataagatac acggacacgg tctgctcacc cctggagaga
      121 gcaatgctgt ctgtgtttat ctccgtcacc gaatagcccg cgtagacaga gcctttcctc
      181 agttccgtct gtctcttccc ccgtccggga gatgttgtcg ggttcttctt gtcttccacg
      241 aaggcgacac gctggttccc atgcatgatt atgccacaca gtaaaagctc cggtttcggc
      301 agttccttct tcgcatcgat aggtatgacc ctttgaggat ggaaaaggtt catttcactg
      361 acgacggcat agtcggtggg aaggggttga gcggcctctt ccgcaggtct ctccggcagg
      421 accgtctcct tcggaacgat ccgcggaagc ttcaagcggt cgctcacgga catcgccggg
      481 cagacaacgc cgatacctga caggaccagg gcgacagcca gaaggacgtt caggagggtc
      541 acattgcgcg ggattgaccg cagcaccttc atcatgtcac tttcctcccg ccagcgaaga
      601 caggcgcagc ttcaccatca gttccctggg gtcgcggaag ttccgcaccc gcgtgtccat
      661 ttccctgacg acgatgtaag gtgtatgggt ctcgatgccg aagaggacgt cgctcaaggc
      721 tctcgtgtcg ggcagcgccg cgtcgacggt aatggtcacc acctggaatc tacccgattc
      781 ctccgttttt tccacgcgct cgctggtgat ggaccctccc cgccccgtta tgataccctt
      841 gaccgcctcc tggagagcgg ccgcacacag tgtcggcgtc tgcgcctcga tggtctttga
      901 tgcggcggct cctctcttct ccttcaaggc atcgagcttc ttttcgatcc tgggtctccc
      961 ggcgatcgtc tccatgcact tcatgagcag cctttcccgt acggccctcg tctccctgag
     1021 agagaccatt tccttgcggg cgttcaccaa gccatattga tagacggtga gagcggcgag
     1081 aacgatggtc gcggggatcg cgtatatgag gatcctattt tcctttttca tccttcttca
     1141 ccgtcccttc cgtctttgcc tcctctttct tgaacccttc cagctccatc cgaaggacaa
     1201 aacgctcccc gttgagccgt gcgtccttca tcgtcggtga ggcgaattcg accttcctga
     1261 aaaggtcgga ctgctccagg atcgagagta cccccgtcgc cgaagcggca taaccctcga
     1321 tctccaccgt ctcttccgtg accctcgctc ttgtgagcca caccgttttc ggaaggagca
     1381 aggtcaaccc cttgaggata ttgagcgaca tgggcctctt cttcttgaat tcctccatgt
     1441 gatccaactc gccctgcaat gcttccgcct ccttcttcag ccgttccact tccatcacat
     1501 cctttctgag ggatcgtatc tgcccctcaa tagcggcgat cctgcgcttt tcgatctcaa
     1561 gaggtacgac aacgtaaggg atcagcatca tgacgagaag cgcggcaagt acgagagagg
     1621 cgaccagatg gcccttcttc ttttgccccg caccgccgcg tgcaagatca aaggttgcga
     1681 ggcccgggaa aagggcttca tggagcatgg ccgcgagaga aagggggaca ttcttctgac
     1741 ttcccccgaa gagtgacatc gtttcgctat ccccgatctg tctgtgcgcg atgtccggca
     1801 gcacgtctcc ggccccgaca ccgttctcgg caacaaggta cacgggccgc gacccatcgg
     1861 ctgcgccggc tgcagcaagg gcagccctca catcttcgta gcgcctctgc gggtcctctt
     1921 catcgccgca cgaatcaaag gaaccgtaga aattacccgc caggttatcc tcttcgaccg
     1981 accagccttc gtatccataa gggtcaacgt gaagggacac gaaggtcctg ctcccggcaa
     2041 gacgcccgca caaccgtgcc atctccaccg aaggcgtcga taccttcacg ggcgggagcc
     2101 cctttttcct cagtgccgcc acgcagggat tcaatctctc ttttctggcg gccatgagag
     2161 ttatggcgat cctttcatcc ctttctgcac cgacgacatg atcgtacatg gcgtcggcgg
     2221 ccgtgagggg agtaagcctg tcgatctcgt aagtcactac aggtcccatg ttctctttta
     2281 cggtcgaggg catgtccgca gtccttatga caacccactc cctgggaaca cccaggagca
     2341 ccttcgagcc tctgcgcccc agttccttca ttgcgcggga cgcggcggcg gcaagatcgt
     2401 cgggggccgg aaatccatct ccttcaaggg cgtaccttcc agatcctatg agccgcctgc
     2461 ggttgagaaa gcgctcaccc gctgccgctg acacgcatcc ccgttccatg cagaccgcca
     2521 ggaagcgctc gggcaggatg atgccttcaa cgaggttgca gttcagcgca gcccatattc
     2581 tcaggcacag gggttcaact ttttccctca cggtcctcac gtatgcgatg cctttctcaa
     2641 tggcggggct cagagccaag gatccaattc tcatttcgtt gtctccgcgg ggatcttgta
     2701 atagatgtac tcgtatgtcc ctcccgtcct gacgaggagt gtggcccgga taccgtggcc
     2761 ttccccggcg ccctctctgt aacctgtcga ttcgatggcg tagccgacgg attcagcgac
     2821 gtctatgtaa gatgatacgt tgccgtattc ccctcccatg atgccgcgaa catcatcgat
     2881 ggttttcagc tccgcctgtt tccttcgatc gatgatcctt gccgcccggt cctccgacac
     2941 gccgggcagt gccatgatga cctctttcgc tgcaaaattc acatttatct ttgacgcatc
     3001 cccatagac
//
LOCUS       DAOPOA010000223         3007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35619_length_3007_cov_4.259485, whole genome shotgun sequence.
ACCESSION   DAOPOA010000223 DAOPOA010000000
VERSION     DAOPOA010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35619_length_3007_cov_4.259485"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..2130
                     /gene="acs"
                     /locus_tag="PKJ17_05615"
     CDS             142..2130
                     /gene="acs"
                     /locus_tag="PKJ17_05615"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOD75483.1"
                     /translation="MADATEETRLYPPPKEFVDQAYVKSRDEYEKMWKQSIQDPETFW
                     GGIAKELSWFKPWLKVNREDFAKGEVEWFIGGKTNIAYNCLDAQVEKGKGDKVAILFQ
                     GEPEDDVVKLTYKEMLSMVSKFANVLKKKGVRKGDRIAIYLPMIWQLPVVMLACARIG
                     AVHTIVFGGFSAEALRDRILDAGAKMLITTNGYWRSGKTVSSKANADTACDLCLAQGH
                     TVDKVVVVKRLDNFDVPMKAGRDTWFEEELAAADITDDCPAEMMDAEDPLFILYTSGS
                     TGKPKGVLHTVGGYMVYTYSTFKYIFDYKDKDVFFCTADIGWVTGHSYIVYGPMTNGA
                     TSIVFESVPTFPNPDRFWQIVEKFKVSIFYTAPTAIRALMKEGEKWPQGRNLSSLRLL
                     GSVGEPINPEAWLWYHKYIGGEKCPIADTWWQTETGGILITSLPGAWPGKPGYATLPF
                     FGVDARALQSRPDASKPAVDAPVNEQGELCIGRSWPGMMRGVFGEPERFFNTYFVQQP
                     GFYFSGDGALRDENGYFRLMGRIDDVVNVSGHRMGTAEVEAALNSFNTAVAESAVVGF
                     PHEVKGEDLYAYIILKTGVEGTDALKKELVAHVRKEIGPIASPGKIQFVSGLPKTRSG
                     KIMRRILRKVASGEIDQIGDTTTLADPSVVDDIVKGRQ"
     gene            2208..>3007
                     /locus_tag="PKJ17_05620"
     CDS             2208..>3007
                     /locus_tag="PKJ17_05620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD75484.1"
                     /translation="MDERDPSTSGGTCRLSTTEAYHRQEIDGLGWELTVCNSLHPAGT
                     PMRRVLEKDASYGHLLYDHLSRFMPLMDMTSILEIGAGYGYLMKDFLERNPGLRPCLM
                     DISPALLGKQRETLAGHDVSYVLQDALEADAATLQRFELVILNENLGDFPTAVDVDHR
                     VFEDREGSGDLPWRICRLFDTYNLEISQTPFNVNLGALEMVEKLCSSEVPRIFIGEHS
                     CEAALPPALKPYIPLAFDGNPRRIRLKGHDEYTIKFSHLRRIAEYYGY"
BASE COUNT          698 a          884 c          850 g          575 t
ORIGIN      
        1 acaagtattg tgaaaagatt aacaaaattt tattgacatc accacccggc gcgtttaaaa
       61 tagtctgttc aataagctaa atacgggtag cttaagctga atacgggcag cttcatttaa
      121 aaatcaataa ggaggttatg tatggcagac gcaacagaag aaaccagatt gtatccaccc
      181 cctaaggaat tcgttgatca ggcttatgtg aagagccgtg acgaatacga aaagatgtgg
      241 aaacagtcca ttcaggaccc cgaaacattc tggggcggca tcgcgaaaga gcttagctgg
      301 ttcaagccgt ggctgaaggt caacagggaa gatttcgcga agggcgaagt ggagtggttc
      361 atcggcggca agaccaacat cgcctacaac tgcctcgatg cccaggtcga gaagggcaag
      421 ggagacaagg tcgccatcct cttccagggc gagccggaag acgatgtcgt gaaactgacc
      481 tacaaggaaa tgctttccat ggtttccaag ttcgccaacg tgcttaaaaa gaaaggcgtc
      541 cgcaagggcg acaggattgc catctacctg cccatgatct ggcagcttcc tgttgtcatg
      601 ctggcctgcg caagaatagg cgccgtccat acgatcgttt tcggcgggtt ctccgctgag
      661 gcgctccgcg acaggatcct cgacgccggc gcaaagatgc tcatcacgac gaacggctac
      721 tggcgttcgg gcaagacggt gagctccaag gcgaacgccg acacagcctg tgatctgtgc
      781 ctcgcgcagg gccacaccgt ggacaaggtc gtcgtcgtca agaggcttga caacttcgac
      841 gtcccgatga aagccggtcg cgacacctgg ttcgaggaag aactggcggc tgcggacatc
      901 accgacgact gccccgccga gatgatggac gcggaagatc cgctcttcat cctctacaca
      961 tcaggttcaa cgggtaagcc gaagggtgtt cttcacaccg tcggcggcta catggtctat
     1021 acgtattcaa ccttcaaata catcttcgac tacaaagaca aagacgtgtt cttctgcacc
     1081 gccgatatcg gctgggtaac gggccacagc tacatcgtct acggcccgat gacgaacgga
     1141 gcgacctcga tcgttttcga gtccgtcccg acattcccga accccgacag gttctggcag
     1201 atcgtcgaga agttcaaggt cagcatcttc tacaccgccc ccacagccat cagggccctc
     1261 atgaaagaag gcgaaaagtg gccgcagggc aggaacctct cctcactgag actccttggt
     1321 tccgtcggcg agccgatcaa cccggaagca tggctctggt accacaagta tatcggcgga
     1381 gagaagtgcc ccatcgccga cacctggtgg cagacggaga caggcggtat cctcatcacc
     1441 tctcttcctg gcgcgtggcc gggaaagccg ggctacgcga cacttccatt cttcggcgtt
     1501 gacgcccgcg cgctccagtc acgccccgac gccagcaaac ccgcggtgga cgcgcccgtg
     1561 aacgagcagg gcgagctctg catcggcagg tcatggcctg gcatgatgag aggcgttttc
     1621 ggcgaacccg aaagattctt caacacctac tttgtccagc agccggggtt ctacttctcc
     1681 ggtgacggtg cgttgaggga cgagaacggc tacttcagac tgatgggccg tatcgacgac
     1741 gtcgtcaacg tatccggcca caggatggga accgcagagg tcgaggcagc cctcaactcc
     1801 ttcaacacgg cagtcgcgga atccgccgtt gtcggcttcc cgcacgaagt gaagggcgag
     1861 gacctttacg catacatcat cctgaaaaca ggcgttgagg gaacggatgc tctcaagaaa
     1921 gagctcgttg cccacgtcag gaaagagatc ggccccatcg catctccggg caagatccag
     1981 ttcgtatccg gtctgccgaa gacacgttcc ggcaagatca tgagaaggat cctgagaaag
     2041 gtcgcatccg gcgagatcga ccagatcggc gataccacca cgctggcaga tccgtcagtt
     2101 gtcgacgata tcgtaaaggg caggcagtaa gaccggcccg ccttcttgaa aggcaatgcg
     2161 aggcccccgg gatcataccc gggggcttct ttttgtgagg acagtacgtg gatgagagag
     2221 acccatcaac ctccggcggc acctgccggc tgagcacgac ggaagcgtat cacaggcagg
     2281 agatcgacgg tctggggtgg gagctgaccg tctgcaattc cctccatccc gccggtacgc
     2341 cgatgcgccg tgtcctcgag aaggatgctt cctacgggca tctgctctat gaccatctca
     2401 gccgtttcat gcccctcatg gacatgacgt ccatcctcga gatcggagcc ggctacggct
     2461 accttatgaa ggatttcctg gagaggaacc ccggattgcg tccctgtctc atggacatct
     2521 caccggcgct cctcgggaaa cagcgggaga cactggccgg tcacgatgta tcctacgtgc
     2581 tgcaggatgc cctggaggcg gacgccgcaa cccttcagcg cttcgagctg gtgatcctca
     2641 acgagaacct cggtgacttt ccgacggcgg tggacgtgga ccatcgggtc ttcgaggacc
     2701 gggagggatc cggtgacctg ccctggagga tatgccgtct ctttgacacc tacaacctcg
     2761 agatatcgca gactcctttc aacgtcaacc tcggggctct ggagatggtc gagaagctct
     2821 gttcttcaga ggtgccccgc atcttcatag gggaacacag ctgtgaagcg gcccttcctc
     2881 cggccctcaa gccgtacata cccctcgcgt tcgacggcaa cccccggcgc atccgcttaa
     2941 agggccacga cgaatacacc atcaagttct cccacctgcg ccgcatagcc gaatattacg
     3001 gttacag
//
LOCUS       DAOPOA010000224         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35761_length_2999_cov_3.230978, whole genome shotgun sequence.
ACCESSION   DAOPOA010000224 DAOPOA010000000
VERSION     DAOPOA010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35761_length_2999_cov_3.230978"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKJ17_05625"
     CDS             complement(<1..344)
                     /locus_tag="PKJ17_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75485.1"
                     /translation="MHRSVVMVVLLLGIAMTAGCVSPNLPAGEETVRLPLNDGVPGLG
                     FLSEGEVQILRDINRARASVAVPLLPLKASRGLSLAAKERAEEIAGSSGGDLTTDEER
                     ERLFKRVRRFGA"
     gene            489..1967
                     /locus_tag="PKJ17_05630"
     CDS             489..1967
                     /locus_tag="PKJ17_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD75486.1"
                     /translation="MDQQKDIVIAGGGLSGLSAALSAAEYGMRAIVLEKMPFPGGAGL
                     FPEGSLGIGTRFQKEKGIRTTTDQVFARVMEFHHWRCNASVIRTLLNESGQTIDWLVD
                     HGVRIEGIRTMFPPEKSLQVWHIFEGRGARLVKVLSEHIQKKGVPLLTKAPVKKLLID
                     DRGAITGVEAVDADGRTMRITARAVIVATGGFASNKEMLKKYVPDVSAPGMAKLMYRG
                     PAVDGRTGDGINLALSAGAALAGMDTLAGNSPYLDNEPAIRQFSGPDHMKQMRCALSQ
                     PFLWVNKHGDRFYNESFGSVFSDVYNAMTANGGLMWSIFDDAMRRSMIDNGPLTPFNA
                     IVVPGQKMTALDEGIEKGMASGFAFKADTIEELAEKIGIRPWKLRETIEAVNRYADSK
                     HDPDFSRRPEHLVKFDTRQGPYYALKGLRAFFLTLGGVQVNTRMQALDEKGEPIPGLY
                     VTGQDMGGLYDSTYDLLAEGSASSFALSSGRIAVRSIGEAIR"
     gene            complement(1957..2652)
                     /locus_tag="PKJ17_05635"
     CDS             complement(1957..2652)
                     /locus_tag="PKJ17_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOD75487.1"
                     /translation="MKSLIDEGRELIDAAEKGDVAGASVLIAAGADVEATDTGGWTPL
                     MVAAQAGHTEVIRLLLAAGADVNARDDFEVTPLMVASSGGFNDIVEMFLEAGAGVNDS
                     DENGTTPLMWASTEGHVEAVRVLIERGAEVNARGASGYTALMDASGKGHAEVAGLLTA
                     AKADVNAKDKNGLTALMLAAMMGHEEVVRILIAAGADVNARADGGYTALMDASGKGRE
                     DIARLLREAGAIG"
BASE COUNT          604 a          962 c          853 g          580 t
ORIGIN      
        1 aaggcgccaa accggcgaac gcgcttgaac agacgctcgc gctcctcgtc cgtcgtcagg
       61 tctccaccgc tcgatccggc aatctcctcc gcgcgttcct ttgcggccag cgaaagtcct
      121 cgcgatgcct tgagcggcag aagaggaact gccacagacg ctcttgccct gttgatgtcc
      181 cggagtatct gcacctcgcc ttccgacagg aaaccgagac cgggtacacc atcatttaga
      241 ggtagcctca ccgtttcctc tccggcaggc aagttcggag agacacatcc cgccgtcatc
      301 gcaatgccca ggagcaagac caccattacc accgatctgt gcatatccct ccctctttct
      361 ctccgtaccg cgcggagaac actctccatt gatttgcacc accgatgtat tatagcatga
      421 atgatgcttc gggcgaggac cgctttgcgc ggcaacaaaa agatgaacga agcgggagga
      481 aatccatcat ggaccagcaa aaggacatcg tcatcgcggg agggggttta agcggcctgt
      541 cggcggcact gtcggcggca gaatacggca tgagggcgat tgtcctcgag aaaatgcctt
      601 ttcccggagg cgccggtctt ttcccggagg gctctctcgg catcggaaca cggtttcaaa
      661 aggaaaaggg gataaggacg acgacggacc aggtcttcgc cagggtaatg gaattccatc
      721 actggcgctg caatgcctcg gtcatcagga ccctgctcaa tgagtccggg cagaccatcg
      781 actggctcgt ggaccacggg gtcaggatag aagggatcag gacgatgttc ccgcccgaga
      841 agagcctcca ggtgtggcac atcttcgagg gccggggagc ccggctcgtg aaggtactct
      901 cggaacacat acagaagaaa ggggtacccc ttctcacgaa ggcaccggtc aagaaactcc
      961 tcatcgacga caggggcgcg atcacgggcg tcgaggccgt cgatgccgat gggcgcacca
     1021 tgaggataac cgcgcgggcc gtcatcgtcg cgaccggggg tttcgcgagc aataaggaga
     1081 tgctgaagaa gtacgtgccg gatgtcagcg cgccgggaat ggcaaaactc atgtaccggg
     1141 gacccgccgt cgacggcagg acgggtgacg ggatcaacct tgccttgagc gcaggcgctg
     1201 cgcttgccgg catggacacg ctcgcgggaa acagccccta cctcgacaac gagccggcca
     1261 tccggcagtt cagcggaccg gaccacatga agcagatgcg ctgcgcctta agccagccct
     1321 tcctctgggt caacaagcat ggagaccgct tttacaatga atctttcggc tccgtcttca
     1381 gcgacgtata caacgccatg accgcgaacg gcgggctgat gtggtccatc tttgacgatg
     1441 caatgcgaag gtccatgatc gacaacggtc ctctcacccc cttcaacgcc atcgtcgttc
     1501 caggacagaa gatgacggcc ctcgatgagg gcatcgaaaa aggtatggcc tccggtttcg
     1561 ccttcaaggc ggacacgatc gaagagctcg cggagaagat agggataaga ccgtggaaac
     1621 tgcgggaaac gatcgaagcg gtcaaccgct acgccgacag caaacatgac ccggacttca
     1681 gccgcagacc ggagcatctc gtgaagttcg ataccaggca gggcccctac tacgcgctga
     1741 aaggtctccg cgccttcttc ctgaccctcg gcggagttca ggtgaatacg cgcatgcagg
     1801 ccctcgatga aaaaggggaa ccgatcccgg gcctgtacgt cacggggcag gacatggggg
     1861 gtctctacga cagcacctac gacctcctcg cggaaggttc ggccagcagc ttcgcgctga
     1921 gctccggccg catcgccgtt agaagcatcg gggaggctat ccgatagccc ctgcttccct
     1981 gagaaggcgg gctatatctt cgcggccctt gcccgaagcg tccatgagcg cggtgtaccc
     2041 gccatctgcc ctcgcgttca cgtcggctcc cgccgctatg aggatccgca ccacctcctc
     2101 gtgacccatc attgccgcga gcatgagtgc cgtcaggccg ttcttgtctt tcgcgttcac
     2161 gtcggccttc gccgccgtga gaagcccggc cacctccgcg tgccctttcc ccgacgcatc
     2221 catgagcgcg gtgtacccgc tcgcgcccct tgcgttcacc tccgctcccc gttctataag
     2281 gacccgcacc gcctccacat gcccttccgt ggacgcccac ataagagggg tcgttccatt
     2341 ctcgtcgcta tcgttcacac ccgccccggc ctcgaggaac atctcgacga tatcgttgaa
     2401 acctcccgat gaggccacca taagaggcgt cacctcgaaa tcatccctcg cgttcacgtc
     2461 cgctcccgcg gccagtagca gccggatcac ctcggtgtgt cctgcctgcg ccgccaccat
     2521 gagaggggtc cagccgccgg tatccgttgc ctccacgtcg gcgcctgccg cgataagcac
     2581 gctcgccccg gcaacgtcac ccttttccgc cgcgtcgata agttcccgac cttcgtcgat
     2641 cagacttttc attgcaaacc tctcccgtat tcgtcccgtc gtctttcaga cccgctatgg
     2701 gcatttgggt tttaacattg agccggaccg gttatcaagc ataaattccg gagcgcaggc
     2761 gcccccggtg ccggcacacg gccgattcgg tgggtcctgg cgagttccgg caagaagaca
     2821 aggcatgtgg ttgaccggaa atggcggttc cttctgtgga aggctgctat gtcttgatgt
     2881 atcccctgtt ctccgccctg tctattatct gctgaatgat cttgtccttg tggtgttgcg
     2941 tgtcgctgac cacttcctgc ataagggtct ggtattccac gtcgtcggtc ttcacgatc
//
LOCUS       DAOPOA010000225         2997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35782_length_2997_cov_4.058124, whole genome shotgun sequence.
ACCESSION   DAOPOA010000225 DAOPOA010000000
VERSION     DAOPOA010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35782_length_2997_cov_4.058124"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKJ17_05640"
     CDS             <1..810
                     /locus_tag="PKJ17_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD75488.1"
                     /translation="YPIHSYSVVIAGGDLRTIPLLPREEFFERLNIAVKTTWPLPKML
                     IISFPNNPTTEVVEKDFFERTVAFAKEHDMMVVHDLAYADLAFDGYRAPSFLEVPGAK
                     DVGVEFFSMSKSYSMPGWRVGFAAGNKRMINALSRIKSYFDYGVFQPIQIASIIALNE
                     GDADVPEIVEKYRARRDVLCEGLCRLGWEVEKPRATMFVWAKIPEKYAAMGSIEFSKF
                     LLTEAKVATSPGIGFGEFGEGYVRFALVENEHRIKQAVKGIRNLLRPTGRS"
     gene            807..2093
                     /locus_tag="PKJ17_05645"
     CDS             807..2093
                     /locus_tag="PKJ17_05645"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOD75489.1"
                     /translation="MIGIGIIGLGTVGTGTYRILTEHAALIRRRTGLDLRVVRVAEVD
                     ERKLRGKGLARNVVTSDAMELIHDERVGIVVELIGGMRPASDFIGAALGLGKQVVTAN
                     KALLAEKGDELFRLAAKKGCEIGFEASVCGGIPVIRAIRDGLVGNDFSYILGILNGTS
                     NYILSRMTDEGISFAEALKDAQRKGYAEKDPTFDVEGIDAAHKLSLLVRLAFASPVTM
                     DRITTRGIAKIDPVDIGFARDFGYKIKLLAIAKKRGAGIEARVEPAMLPVNHPMSSVN
                     GVYNAVYIVGDMTGPNLFYGKGAGSDPTGSAVVSDIVDIAKRISGAGGTGVRPLVAAG
                     RFKEGRASSVPYYLRVMAKDMPGVLSKISGILSKYRISISAVTQQGRKLSGYVPVVMV
                     THDAPEEGLMKAKKEIDALSLTKGESVHIRIEEGNL"
     gene            2134..>2997
                     /gene="apgM"
                     /locus_tag="PKJ17_05650"
     CDS             2134..>2997
                     /gene="apgM"
                     /locus_tag="PKJ17_05650"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOD75490.1"
                     /translation="MKYIVLIGDGMADFPLEELGGKTPLMAANKPNMNAAARKGLTGK
                     VKTVPDGFPPGSDVAGMSIFGYDPALYYTGRAPIEAFGMGIPMGSDDVAYRCNLVHIG
                     TGNGGDTMGDYSGGHISTEEARILVASLNDELGSEGITFFPGVGYRHIMIWKRGAWEL
                     STTPPHDITGKQIAGYLPEGDGAAMVRSLMDRSRRVLGSHDINRKRIDAGKLPANGIW
                     LWGQGKRAGLPPFREKFGLTGATVCAVDLVRGISSLVGFTTPVVEGATGYLDTDYGAK
                     ARAALKLLEDHD"
BASE COUNT          637 a          873 c          983 g          504 t
ORIGIN      
        1 taccccatcc actcctattc tgtggtcatc gccggcgggg acttgaggac catcccgctt
       61 ctaccaaggg aggaattctt cgaaaggctg aatatcgcgg tgaagaccac gtggcccctg
      121 ccgaagatgc tcatcatcag ttttcccaac aaccccacca cggaggttgt ggaaaaggac
      181 ttcttcgaaa ggaccgtcgc cttcgcgaag gagcatgaca tgatggtcgt ccacgacctg
      241 gcgtacgccg atctcgcctt cgacggctac agggcgccga gcttcctcga ggtcccgggc
      301 gcgaaggatg tgggcgtgga gttcttttcc atgtccaaga gctacagcat gcccggctgg
      361 cgggtcgggt tcgcggcggg caacaaaagg atgataaacg ccctcagcag gataaagagc
      421 tacttcgact acggcgtctt tcagccgatc cagatcgcgt ccatcatcgc cctcaatgaa
      481 ggcgacgcgg atgtgcccga gatcgtggaa aagtacaggg cgcgacggga cgtgctttgc
      541 gagggcctct gccgcctcgg gtgggaggtg gagaaaccga gagccacaat gttcgtctgg
      601 gcaaagatac ctgagaagta cgcggcgatg gggtccatcg agttctcaaa gttcctcctg
      661 acagaggcaa aggtggccac ctcgccgggg atcggcttcg gcgagttcgg tgaaggatac
      721 gtgcggttcg ccctcgtgga gaacgagcac agaataaaac aggcggtgaa ggggatcagg
      781 aatctcctcc gccctacggg aagatcatga tcggaatagg gataatagga ttgggaacag
      841 tgggaaccgg cacataccgg atactgacgg aacacgccgc gctcattcgc aggaggacag
      901 gtctcgacct tcgcgtcgtc agggtggccg aggtggacga gcgcaagctg agaggaaaag
      961 gccttgcacg gaatgtcgtg acctccgatg ccatggagct catccatgac gagagggtcg
     1021 gcatcgtcgt ggagcttatt ggcggcatgc gtcccgccag cgacttcatc ggcgcggccc
     1081 tcggtctagg caagcaggtt gtgacggcaa acaaggccct cctcgcggaa aaaggcgacg
     1141 agcttttcag gctcgcggca aagaaggggt gcgagatcgg gttcgaggcc tccgtgtgcg
     1201 gaggcatccc cgttatccgg gcgatccgcg acggtctcgt cggcaatgat ttcagctaca
     1261 tactgggaat cctcaacggg acgagcaact acatcctctc gcggatgacc gacgaaggga
     1321 tatccttcgc cgaggccctc aaggacgccc agcggaaagg ctacgctgaa aaggacccca
     1381 ccttcgacgt cgaggggata gacgccgcgc acaagctttc gctcctggta aggctcgcct
     1441 tcgcctcgcc tgtcaccatg gacagaataa cgacacgggg catcgcgaag atagaccccg
     1501 tcgacatagg cttcgcccgg gacttcgggt acaagataaa gctccttgcc atcgcgaaga
     1561 agcggggggc aggcatcgag gctcgcgtcg agccagccat gctgcccgtg aaccacccca
     1621 tgagcagcgt caacggcgtc tataacgcgg tctacatagt cggggacatg acgggcccca
     1681 atctcttcta tggaaagggt gcggggagcg atcccacggg aagcgccgtc gtgagcgaca
     1741 tcgtcgacat cgccaagcgg atctccgggg cgggcggcac aggcgtgcgg cccctcgtcg
     1801 cggcgggcag gttcaaggaa ggcagggcct cttccgtgcc ctactacctg cgggtgatgg
     1861 caaaggacat gccgggtgtg ctttcgaaga tatcgggcat cctctcgaag tatcggataa
     1921 gcatatcggc ggtgacccag cagggccgga agctcagcgg ctacgtcccc gtcgtgatgg
     1981 tgacccacga tgcccccgag gaaggtctca tgaaggcgaa gaaggaaata gacgccctgt
     2041 ccctgacgaa gggggagagc gttcacataa ggatcgagga aggcaacctg tgagaggacc
     2101 ggggccttcc ggccgcgtga ggtgcggcag cgcatgaaat atatcgttct catcggcgac
     2161 ggcatggcgg attttccgct ggaggagctc ggggggaaga cgccgctcat ggccgcgaac
     2221 aagccgaaca tgaacgcggc ggcccgaaag ggtctcacgg gaaaggtaaa gacggtgccc
     2281 gacggttttc ctcccggcag cgacgtggcg ggtatgtcca tcttcggcta cgacccggcg
     2341 ctgtattaca cggggcgggc cccgatagag gccttcggca tgggcatccc catggggtcc
     2401 gacgacgtcg cctaccggtg caacctcgtt catatcggga ccgggaacgg gggtgatacc
     2461 atgggcgact actccggcgg gcatatctcc acggaggagg cacggatcct tgtcgcgtcc
     2521 ctcaatgacg agctgggttc cgaagggata accttcttcc ccggggtcgg gtaccgccac
     2581 atcatgatat ggaaaagagg cgcctgggag ttgtcgacaa cgcctcccca cgacatcacg
     2641 ggcaaacaga tcgcggggta cctccccgag ggcgacggag ccgcaatggt ccgttccctt
     2701 atggaccgct cccgcagggt gctcgggtcc cacgacatca acaggaagcg catcgacgcc
     2761 ggcaagctcc cggcgaacgg catatggctc tggggccagg gcaaaagagc cggcctgcca
     2821 ccgttccggg agaagttcgg ccttacgggg gcgacggtct gcgccgtgga cctcgtgcgg
     2881 gggatctcga gcctcgtggg gttcaccacc cccgtcgtcg aaggggccac gggatacctc
     2941 gacaccgatt acggggcgaa ggcgcgtgcg gccttgaaac tcctggaaga ccacgac
//
LOCUS       DAOPOA010000226         2994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35820_length_2994_cov_3.825791, whole genome shotgun sequence.
ACCESSION   DAOPOA010000226 DAOPOA010000000
VERSION     DAOPOA010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35820_length_2994_cov_3.825791"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..533)
                     /locus_tag="PKJ17_05655"
     CDS             complement(6..533)
                     /locus_tag="PKJ17_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75491.1"
                     /translation="MTLKDKCLKVWQTLRPDQRKRLVFVAVGLAIVIVSLVLYRATRP
                     ANPVLKQNESKRDIALDKGTLEKSLYNESTKQMGDMEAEMKAVKEQLAALQQAKKPEN
                     DDNADLAKEILKTSRQGLSEKQTEPTKPAKGSTPPRPASYPPGIPPLPAAPSSPPTPT
                     APAIRLHQNRRFTGR"
     gene            complement(530..1294)
                     /locus_tag="PKJ17_05660"
     CDS             complement(530..1294)
                     /locus_tag="PKJ17_05660"
                     /inference="COORDINATES: protein motif:HMM:NF018311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HOD75492.1"
                     /translation="MRPQILTGGFDDVPASRDDQATIPDLPQRITPEGPSQVVLSSSD
                     INRIICPVDIKDAIYSKEKGLTVRLAGQNAFVKFLVMKKDGKDLYATTPSELYVVCGE
                     NVYTMIAVPRRIPAQTVQLYGGKVEAIKKNMSLFSEVPFEKRIITIIKSIYTDRIPDS
                     FAIRNMDRSCPVFQGLSLVLHRLFTIDGEGLAIKEYRARIADGSKENAVTLREKDFLT
                     TDLAQRPVAVSIDTLNLKRGETSRIFVVERTEGGRP"
     gene            complement(1600..2178)
                     /locus_tag="PKJ17_05665"
     CDS             complement(1600..2178)
                     /locus_tag="PKJ17_05665"
                     /inference="COORDINATES: protein motif:HMM:NF017152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HOD75493.1"
                     /translation="MELNKYIEQKNNLIAGNRLLKLAVVTVGLGLIVNAFITFSLSKR
                     ARTVIVPPIVNTRFEISGSRLSDEYVKLMTRYVMSLAANYTPITARSNFDELLGLYDP
                     ASYAEGKRTLYKLADTVETAHVTGSFFIQKITVDENKREILVEGMKRLTANDQKVEEG
                     PETYIVAYGNDNGRFSIANLSRKPRERGETVR"
     gene            complement(2191..2457)
                     /locus_tag="PKJ17_05670"
     CDS             complement(2191..2457)
                     /locus_tag="PKJ17_05670"
                     /inference="COORDINATES: protein motif:HMM:NF018836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HOD75494.1"
                     /translation="MKKRFPQYLTQPFMILSFEPDEQMITLTCVVVALTVWGWAVLLP
                     FTVPYLYVKTKKKYPRGFIKHVFYILGFVRFDGYPTFHEREFLE"
     gene            complement(2518..2835)
                     /locus_tag="PKJ17_05675"
     CDS             complement(2518..2835)
                     /locus_tag="PKJ17_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75495.1"
                     /translation="MPWRTAISGQSLVMLMAVAVLLLGSALPSFAITAPTSGSLFYDV
                     YDIVVNSMLKGAIGFVAAILVFMMGIGLFFVQKIIPGIIGVVCAALIIKADAIVTTLG
                     LTF"
BASE COUNT          554 a          810 c          871 g          759 t
ORIGIN      
        1 gaactctatc tccccgtaaa cctccggttt tgatggagcc gaatagccgg cgctgtcggc
       61 gttgggggag acgacggagc ggcgggaagg ggcgggatac cggggggata tgacgcgggg
      121 cgtggaggcg tgctaccctt ggcgggcttt gtcggctcag tctgtttttc ggaaagaccc
      181 tgccgcgaag tcttcagaat ctctttcgcc agatccgcat tgtcgtcatt ctcgggcttt
      241 ttggcctgct gcaatgctgc aagctgctcc ttgacggcct tcatctcggc ctccatgtct
      301 cccatctgtt tcgtggattc gttatagagg gatttctcca gtgttccctt gtcgagggcg
      361 atatcccttt tgctctcgtt ttgcttaaga acagggtttg cgggcctggt tgccctgtaa
      421 agcaccaggg agacgatgac gatcgcgagt ccgacggcga cgaagacgag tctctttctc
      481 tggtcgggcc gcaatgtttg ccaaactttc aggcatttgt ctttgagcgt cacggcctgc
      541 ctccttccgt cctttcaacg acgaagatcc tcgatgtctc acctctcttg aggttcaggg
      601 tatcaatgct cacggctacg ggcctctgtg ccaggtcggt ggtgaggaaa tccttttcgc
      661 gcaaggtaac ggcgttctcc ttcgagccat ctgctatcct tgcccggtat tccttgattg
      721 cgagaccctc tccgtctatc gtgaataacc tgtggagcac gagggaaagc ccctggaaga
      781 cgggacagga tctgtccata ttccggatgg cgaaggaatc gggaatccga tccgtataga
      841 tgcttttgat gatggtgatg atcctcttct cgaacggtac ttcgctgaaa aggctcatat
      901 tctttttgat ggcttcaact ttgcctccat agagctgcac ggtctgtgcg gggatgcgcc
      961 ggggtacggc gatcattgtg tagacgttct cgccgcagac gacgtatagt tccgagggtg
     1021 ttgtcgcgta gaggtccttg ccgtccttct tcatgacgag gaacttgacg aaggcattct
     1081 ggcctgccag cctgacggtt agccctttct ccttcgagta gatggcatcc tttatgtcga
     1141 cgggacagat gatcctgttg atatcggagc tgctcaggac cacctgggaa ggcccttcgg
     1201 gagtgatcct ctgcggcagg tcggggatgg tcgcttggtc atcccgggaa gccggcacat
     1261 cgtcgaagcc gcctgtgaga atttgcggcc tcatgtcgtc gttccttgag gcctttgttt
     1321 gccgaggcgt agcggtttcg ggccgtgcga gggcggccga ggccggcggg ggtggtgtcc
     1381 cgacgggcat cgtctttcct ggttgccccg aggcaggcgg gttttggacg ccttgggtcg
     1441 gggtcagctt cggataattc gactgcacca cctcgggttt ttgtcctgcg gcaaggccag
     1501 gcatagtgca attcccggtg ctgcacggct cggcttccgc gaagccaaga aaggcggtca
     1561 tgataaagag aacggcgaat agtagtgtct tcagtcggtt catcgtactg tttctcctct
     1621 ctcacgtggt ttgcggctca gattggcaat tgagaagcgg ccgttgtcgt tcccgtaagc
     1681 gacgatgtag gtctccggtc cttcctccac cttctgatcg ttggccgtca atcgcttcat
     1741 gccttcaacc agtatctccc tcttgttctc gtcgacggtg atcttctgaa tgaagaagga
     1801 accggtcacg tgtgccgtct ccacggtatc ggcaagtttg tagagcgtcc ttttgccttc
     1861 cgcatatgac gcgggatcgt agagacccag gagttcgtcg aaattgcttc tcgctgtgat
     1921 gggcgtgtag tttgccgcca agctcatgac atagcgggtc atgagtttca cgtactcatc
     1981 cgacaacctg gagcccgata tctcgaaccg cgtgttgacg atgggaggga cgatgacggt
     2041 cctcgcccgt ttcgagagac tgaaggtgat gaaggcgttg acgatcaggc cgagccctac
     2101 ggtaacaacg gccagtttga gcagccggtt gccggcgatc aggttgtttt tctgttcgat
     2161 gtacttgttc agctccatgc tgcgacccct ctattccaga aattcccgtt cgtgaaaggt
     2221 cgggtacccg tcaaacctca caaatccgag gatgtagaag acatgcttga tgaaaccccg
     2281 ggggtatttc ttcttcgtct ttacgtagag gtaggggacc gtgaagggaa gaagaaccgc
     2341 ccatccccat acggtcagcg ccacgaccac gcaggtgagc gtgatcattt gctcgtcggg
     2401 ctcgaacgac aggatcatga agggctgtgt caggtactgg gggaatctct tcttcacggt
     2461 ctttacctct atgaatgggc gagacggttc gtgactatcc cgcccatccg gttcctttta
     2521 gaatgtcagt ccaagggttg ttacgattgc gtccgccttg atgataagcg cggcgcatac
     2581 cacaccgatg atgccgggga tgatcttctg cacgaagaag aggcctatcc ccatcatgaa
     2641 tacgaggatg gccgccacaa agcctattgc gcccttcagc atgctgttga ccacgatatc
     2701 gtagacatcg tagaagagac tccccgaggt cggggccgtg atcgcaaagg acggcagggc
     2761 gctgccgaga agcaacacgg cgaccgccat tagcattacc agggactggc ctgagattgc
     2821 cgttctccac ggcattggcc ggcgcgattc cgtcctaggg cgcacccgcg gcaacacgac
     2881 ggtccccttc atacactcca tcactttttt caactgtctc ataaaccttt ctccttatct
     2941 gttggtgttg ttttggttct tcaggatctt ctcgatgacc gggatattcg cccc
//
LOCUS       DAOPOA010000227         2990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_35882_length_2990_cov_4.957411, whole genome shotgun sequence.
ACCESSION   DAOPOA010000227 DAOPOA010000000
VERSION     DAOPOA010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35882_length_2990_cov_4.957411"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PKJ17_05680"
     CDS             complement(<1..396)
                     /locus_tag="PKJ17_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOD75496.1"
                     /translation="MDILNSTIAQLREMLKKKEISSVELTRYYLDRIGKYNGEILAYL
                     RTTDDYAMKMAREADEKIARGEDAPLLGVPLGMKDILCTKGIETTCASSILKGFVPPY
                     DATVIRKLKEAGFVHLGRLNMDEFAMGSST"
     gene            complement(546..842)
                     /gene="gatC"
                     /locus_tag="PKJ17_05685"
     CDS             complement(546..842)
                     /gene="gatC"
                     /locus_tag="PKJ17_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOD75497.1"
                     /translation="MKITRETVEYVAHLARLDLDAGEVDAYTRQIDSILEYMDSLNAL
                     DTEGVEPTSHPVPVGCVMREDAVRDSFTAEESVGNAPARIDTFFKVPPIIEVEE"
     gene            complement(1112..1483)
                     /locus_tag="PKJ17_05690"
     CDS             complement(1112..1483)
                     /locus_tag="PKJ17_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOD75498.1"
                     /translation="MKVVELMNKDVVTCHPTEKLNIILNKLELFNIAGMPVVDKGKLV
                     GIVCQSDILRGLKNGSMTELSVSDVMTTDVITVPPTESAVLVAKIMVEKHINRVPIVE
                     NDKVVGIVTRGDIIKAVAECG"
     gene            complement(1495..2796)
                     /locus_tag="PKJ17_05695"
     CDS             complement(1495..2796)
                     /locus_tag="PKJ17_05695"
                     /inference="COORDINATES: protein motif:HMM:NF021824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HOD75499.1"
                     /translation="MPLNFSLIESFIADHQKDMRNKAMLRHDRYRVLSELGTALQTVL
                     KDYEIFETELNALVIEIIDNTSSYETLRECHERAVAGVENFFLEEDTIVDVHDLLRII
                     RDRIAIRVLELVEKEMIGEGLGKPPVDYVWAGLGSEGRDEQTLVTDQDNLLVYAETDD
                     GFASDELRKRCAAMMTEGPVPPNALDAKTLLDGYFRIFAGKASDRLHQVGFEKCKGGV
                     MPVNDRWRGSIKDWKKRIDERMTFGTGIFEPLDVIILTDARRVNGSQAILGELLDYFF
                     ALLTNNKHFMKEFIQSAVLMPSALTFFGGFKVEKEGENKDKFNIKLLGWSPLILSVRM
                     LSLSNGIYERNTLKRIAILRQRNIIKKEMESSLTDAYFTFVRYRIINQINYRDDEGKI
                     SNFLRPDMLGPDEQERLRRAMKAVEAFQKFIQETLLFGEAV"
     gene            complement(2800..>2990)
                     /locus_tag="PKJ17_05700"
     CDS             complement(2800..>2990)
                     /locus_tag="PKJ17_05700"
                     /inference="COORDINATES: protein motif:HMM:NF021941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75500.1"
                     /translation="IEKELARITEDSEKLMKKLGNEAFRQKAPAEVIARNEAQYSELS
                     AKREKLIQSRKVLENLSR"
BASE COUNT          558 a          911 c          782 g          739 t
ORIGIN      
        1 ggtggatgag cccatggcga actcgtccat gttgagcctg ccgagatgga cgaatccggc
       61 ctccttcagc ttcctgatga ccgtggcgtc ataggggggc acgaagcctt tcaggatact
      121 cgaggcgcag gtggtctcga tccccttcgt gcacaggata tccttcatgc cgaggggaac
      181 gcccagaagg ggcgcgtctt ctccgcgcgc gatcttctca tccgcctcgc gcgccatctt
      241 catggcgtaa tcgtccgttg ttctcagata agccagtatc tcgccgttgt acttccctat
      301 gcggtcaaga taatacctcg tcagttccac ggagctgatc tctttcttct tcagcatttc
      361 cctcaactga gctattgtcg aattcagtat atccacgcct catctcctcc aaacgaacca
      421 catcatggct gcacattctt cccgaatccc gtcccgcgcc ggaatgacgc aggagtcaat
      481 tcgagcgcct tcctgtttct caacgctcaa gcggctcaag caattcaagc ggctcaagcc
      541 ggttcttact cctccacctc tatgatcggc ggcaccttga agaaggtgtc gatccttgcc
      601 ggggcgttgc ccaccgattc ctccgcggta aaggagtccc tgaccgcgtc ctcccgcatg
      661 acgcatccca ccggaacggg atggctcgtg ggctccacac cctccgtatc gagggcgttc
      721 agcgagtcca tgtactcgag gatgctgtct atctgtctcg tataggcatc cacctccccg
      781 gcgtcgagat caagcctcgc aagatgcgcc acatactcaa cggtctctct tgttatcttc
      841 atgtcagtcc tttctcaagt ctcaagtcac gggttgcagg tcccaagtcg cgggtcggga
      901 gaccaggaca gaccgtcacc gtccacaccg ttacaagtat attcccgcaa gtatctgaaa
      961 ccacttcccg ggtatctcgt ggtagctcgc ctcaacgttc cgtcgcgtca gcctgcaacc
     1021 tgaaacgttg aacctgaaac cgtcttgtcg tccataccgt tcccggcttc agtgtcttca
     1081 aaggcctgag actgaaacct gagacgcttg tttatccgca ctccgccacg gccttgatga
     1141 tgtcgccgcg ggtgacgatc ccgaccactt tgtcgttctc gacgatgggc accctgttga
     1201 tatgcttctc caccatgatc ttcgccacga gcaccgccga ctccgtgggc ggcacggtga
     1261 tgacatccgt cgtcatcacg tcggacacgg aaagctcggt catgctcccg ttcttgagcc
     1321 ccctgaggat gtcgctctgg cagacgatgc cgacgagctt tcccttgtcc acgacgggca
     1381 tccccgctat gttgaagagc tccagcttgt tgagtatgat gttcagtttc tccgtcggat
     1441 gacaggttac cacgtctttg ttcatcagct ccacgacctt cataataacc ccctttatac
     1501 tgcctctccg aagaggagtg tctcctgtat gaatttctga aaggcctcca cggccttcat
     1561 cgccctgcgc agtctttcct gctcgtcggg gcccagcatg tcgggccgaa ggaagttcga
     1621 gatcttgccc tcatcgtcgc gataattgat ctggttgatt atccggtagc ggacgaaggt
     1681 gaagtacgcg tccgtaaggg aactctccat ctccttcttg atgatgttgc gctgcctcag
     1741 gatcgcgatc cgtttgagtg tgttcctctc gtatatcccg ttagacaggg agagcatccg
     1801 gacggagagg atgagcggcg accagccgag gagcttgata ttgaacttgt ccttgttctc
     1861 cccttccttt tcgaccttga aacctccgaa gaaggtgagc gcgctcggca tcaggacggc
     1921 ggactggatg aactccttca tgaagtgctt gttattggtg aggagggcga agaagtagtc
     1981 cagaagctcc ccgaggatcg cctggctgcc gttgacccgg cgggcgtcgg tgaggatgat
     2041 gacgtcgagg ggctcgaaga tccccgttcc gaaggtcatg cgttcgtcga tcctcttctt
     2101 ccagtccttt atcgaacctc tccaccggtc gttgacgggc atgacgccgc ccttgcattt
     2161 ctcgaatccg acctggtgga gccggtcgga ggccttcccg gcgaatatcc ggaaataccc
     2221 gtcgaggaga gtcttggcgt ccagggcatt tggcgggaca gggccttccg tcatcattgc
     2281 ggcacagcgt tttctcagtt catcggaggc gaaaccatca tcggtttcgg catagacaag
     2341 gagattgtcc tgatcggtga ccaacgtctg ttcgtcgcgg ccttcgcttc ccagccctgc
     2401 ccagacataa tccaccggag gcttgcccag gccctcgccg atcatttctt tctccacgag
     2461 ttcgaggacc ctgatcgcga tcctgtcccg gatgatgcga aggagatcgt ggacatccac
     2521 gatcgtgtcc tcttcgagga agaagttctc cactccggct acggcccgtt catggcattc
     2581 ccgaagggtt tcgtaggacg atgtgttgtc gattatctct atgacgagcg cgttgagctc
     2641 ggtctcgaat atctcgtagt ctttgaggac ggtctgcagc gccgtcccca gctccgagag
     2701 aacacggtag cggtcatgac ggagcatggc cttgttccgc atgtccttct ggtggtccgc
     2761 tatgaaggac tcgatgagcg aaaaatttaa gggcatcaac tacctcgaaa ggttctcaag
     2821 cacctttctg ctttgtatga gcttctccct cttcgcggaa agctcgctgt actgcgcctc
     2881 gttgcgggct atgacctccg ccggcgcctt ctggcggaac gcctcgttgc ccagcttctt
     2941 catcaatttt tcactatctt cggtgatcct ggcaagctct ttttcgatcc
//
LOCUS       DAOPOA010000228         2975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_36117_length_2975_cov_3.031507, whole genome shotgun sequence.
ACCESSION   DAOPOA010000228 DAOPOA010000000
VERSION     DAOPOA010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36117_length_2975_cov_3.031507"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKJ17_05705"
     CDS             complement(<1..190)
                     /locus_tag="PKJ17_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOD75501.1"
                     /translation="MDRSVPAEAGENLLETAMKAGVHINASCGGRGVCGKCRVRVVGG
                     SLVSPVHPSVGEEGHAAGI"
     gene            440..1189
                     /locus_tag="PKJ17_05710"
     CDS             440..1189
                     /locus_tag="PKJ17_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOD75502.1"
                     /translation="MYSAPVIKKTFEVLSLIVDSKRPLGVTEVARTLALSKSTVFGIL
                     KALQEENLIVKSKSTKRYTIGEELFKIAKRVLQGGELSSIARPFLEKLVENVDETVFL
                     CVREDSVVKVIDTIETRKKLKISSPVGTRLPITASVFCKAFLAPMEDADIRDFLKEKG
                     LPRYTPNSITDLDRFIDEIRVTRKRHYSLDLEEYLMGVRAIATLVYANDYPVGAICIL
                     GFTGSMPTDKLPEMAQHLVNTARRISDRLSQ"
     gene            complement(1283..>2975)
                     /locus_tag="PKJ17_05715"
     CDS             complement(1283..>2975)
                     /locus_tag="PKJ17_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOD75503.1"
                     /translation="KNRNAMAILPLLAVYLVSVAAFLSMSGIAICAETVFRNVIVLVA
                     DGMGSTHTTLTRWYKGAPLSLDAMYVGGVRTYSADSLITDSAPAATALACGFKSTDKS
                     VGIMACAVTIPGVPDPGGSRCKPIASVLEGAKLSGRSVGLVATSNVQHATPAAFSSHW
                     PYRADYSEIARQQVYQDIDVVLGGGRQYLLPADKGGTRKDDENLVNVLRSKGYAFVAN
                     RDEMLKAASPKLWGMFADDALAYDLDRKSLSRDEPSLSEMTGKAMEVLSKNPKGFFLF
                     VEGSKIDWASHANDPVGVISDVLAFDEAVGTAVDFARRSGGTMVIALSDHGNGGMSIG
                     NRGTDKAYSRLRHERVVGPLRKVSLTGEGIEKALKDDRSEDNVRKVIGKYLGIDDLTP
                     GEMAAVRSAKPGKMNEATGPLVSTRSCVGWTTHGHTGEDLFFYYYGMDRPLPTMENSD
                     IARVMAKAMGFDLADADRRLFVPAEEVFKRIGASVTLDRRDPRNMVLIVESEGKRAEL
                     PLSKNTMRIEGRTKTTRTLEGITVLAPATGKVYIPRQAVEIFENVQGFKSSKVQE"
BASE COUNT          624 a          950 c          763 g          638 t
ORIGIN      
        1 ggatgcccgc ggcatgcccc tcttcaccga cagaaggatg gacgggcgaa accagtgaac
       61 ctccgaccac ccgcaccctg cattttccgc agactcccct ccccccgcag gaggcattga
      121 tatgcacccc cgccttcatg gctgtttcga gtaagttctc tcctgcttcc gccggcacac
      181 tccggtccat cggcagaaat cttattctgt attctggtgg catggtcttt agacacatca
      241 ttgtaccaaa ttctcccatc tttgaacata ccctaatttc cagggcggcc gctcctgctt
      301 cggcttccca aaaggcccgt gttcactatc ataaatgcca accggctatt tggatacctc
      361 ccttcttaaa gcagtattat caacaaatta acgttgacaa aagggccctt atattatata
      421 gtgttcacca tcgtgaatta tgtacagcgc gcccgtcata aagaagacct ttgaggtcct
      481 ttcgctcatc gtggacagca aaagaccgct gggtgtcacg gaggtggctc ggacactcgc
      541 cctcagcaag agcaccgtgt tcggtatact caaggccctg caggaggaga acctcatcgt
      601 gaagagcaaa tccacgaagc gctacacgat aggcgaggaa ctcttcaaga tcgcgaagag
      661 ggtccttcag ggcggtgagc tgagctcgat cgcccgcccc ttcctcgaga agctcgtcga
      721 gaacgtcgac gaaacggtct ttctctgtgt ccgcgaggat agcgtcgtca aggtcatcga
      781 caccatagag acccgcaaga agctcaagat atcgtccccc gtcggaacac ggctgccgat
      841 aacggcatcg gtattctgca aggccttcct cgcccccatg gaggacgccg acatccggga
      901 ctttctcaag gaaaaaggcc tgcccagata cactccgaac agcatcaccg acctcgaccg
      961 tttcatcgac gagatacggg tgacacggaa acgccactac agcctcgacc tcgaggaata
     1021 tctcatgggt gtcagggcga tagccaccct cgtctacgcc aacgactatc ccgtcggagc
     1081 catctgtatc ctcggcttca ccggctccat gcccacggac aaactgccgg aaatggctca
     1141 acacctcgtc aacaccgccc gcaggatatc cgacagactg tcacagtaaa gacggttcaa
     1201 gagttcaaga attcaagaga aagaccgcga agagtggtcg gctgtcaacg caaggactga
     1261 cccgggcaca tagtctgcct ttctactctt gaacctttga acttttgaac ccttgaacat
     1321 tctcgaagat ctccaccgcc tgccggggga tgtagacttt gccggtcgcg ggggcgagga
     1381 cggtgatgcc ttccagggtg cgggtggttt tcgtccttcc ctctattctc atggtatttt
     1441 tgctcagcgg caactcggct ctcttcccct ccgattcgac gatgaggacc atgttgcggg
     1501 ggtccctgcg gtcgagggtc accgatgccc caatcctctt gaaaacctct tcggcgggga
     1561 cgaagagccg cctgtccgca tcggccaggt cgaatcccat cgcttttgcc ataacgcggg
     1621 cgatatcgga gttctccatc gttggcagag gtctgtccat cccatagtaa tagaaaaaaa
     1681 ggtcttcacc cgtatgcccg tgggtcgtcc aacccacgca gcttcgcgtg cttacgagag
     1741 ggcctgtcgc ctcgttcatc tttccgggct tcgcggaacg gacagcggcc atctcccctg
     1801 gtgtcagatc gtctatcccg aggtatttcc ctatcacctt cctcacgttg tcctctgacc
     1861 tgtcgtcctt caacgctttc tcaatcccct cgccggtgag ggacaccttc ctgaggggac
     1921 cgacaaccct ctcgtgccgc aaacgggagt acgccttgtc cgtcccccgg ttcccaatgg
     1981 acattcctcc gtttccatgg tccgacagcg cgatcaccat ggtaccacca ctcctgcgcg
     2041 cgaaatcgac ggcagtcccg acggcctcat cgaaggcaag gacatcgctt atcaccccca
     2101 cggggtcgtt ggcgtgagag gcccagtcta tcttgcttcc ttccacgaac aggaagaagc
     2161 ctttcgggtt cttcgacagg acctccatcg ccttacccgt catttccgac aggcttggtt
     2221 cgtcccgcga gaggctcttt ctgtcgagat cgtacgccag cgcgtcatcc gcgaacatgc
     2281 cccagagctt cggggatgcg gctttcagca tctcatccct gttcgccacg aaagcatagc
     2341 ctttcgaccg gaggacattg acgaggttct cgtcgtcctt tcgcgtacca cccttatccg
     2401 cgggaagcag gtattgcctc ccgcccccca ggaccacgtc gatgtcctgg tatacctgct
     2461 gtctcgcgat ctcgctgtaa tcggccctgt agggccagtg ggaagagaag gcggcaggcg
     2521 tcgcatgctg gacgttggag gtcgccacaa gaccgacgga cctgccactc agtttcgctc
     2581 cttccagcac ggacgctatc ggtttgcacc gggaccctcc gggatcgggg acccccggga
     2641 tggtcaccgc gcaggccatt atgcccacgg acttgtccgt ggatttgaag ccgcatgcga
     2701 gggccgtcgc ggcaggtgcc gagtcggtta tcagcgaatc ggcgctatag gtccgcaccc
     2761 ctcccacata catagcatcc aacgaaaggg gggctccctt gtaccatctg gtcaaagtcg
     2821 tatgggtgga gcccattcca tccgccacaa ggacgatgac gttcctgaag accgtttccg
     2881 cacagatcgc aatgccgctc atcgaaagaa aagcagcgac ggacacgaga tacacggcca
     2941 gcagcggcag tatcgccatc gcattcctgt tcttc
//
LOCUS       DAOPOA010000229         2963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_36312_length_2963_cov_3.266850, whole genome shotgun sequence.
ACCESSION   DAOPOA010000229 DAOPOA010000000
VERSION     DAOPOA010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36312_length_2963_cov_3.266850"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKJ17_05720"
     CDS             complement(<1..496)
                     /locus_tag="PKJ17_05720"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOD75504.1"
                     /translation="MRKGKHLLCKEDGFALIMVLWVIIILMVIVMSFSVLAKTESRAA
                     LFFRDSLAEGFLAQAGLERAILEIYYRQDNLNKTVVPDSGETLRVDGTAIEGDVGDGR
                     YVVRIFNEAGRINLNTMSDGNKIILDNLLVNLGVPKETADTVVDSALDWMDKDVLHRL
                     NGAED"
     gene            complement(493..1140)
                     /locus_tag="PKJ17_05725"
     CDS             complement(493..1140)
                     /locus_tag="PKJ17_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD75505.1"
                     /translation="MRICRDKLSRSGGFTLLELVVSIALVAMIVVILAASMRAGHRSL
                     EGGERKAEWMERLRVSMRLIDSQLQSCLPLTVKEQKDLKRSVFIGAHDSMTFASNRSL
                     FGGRKGYTIASYRIEKDEKGKLALYLKETTIGMKNVKETKLLVGFDDIRFEYLQSAGI
                     KGLGGGDWSDELADTTHLPQKIKLHLEKGARKFSLTIPLRTRKPPGNDPDEESGT"
     gene            complement(1127..1564)
                     /locus_tag="PKJ17_05730"
     CDS             complement(1127..1564)
                     /locus_tag="PKJ17_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD75506.1"
                     /translation="MGRKTERSGRRRCPAGPEGFTLLEILVAFVILATVVTVVLQLFS
                     ANLTTLSMSDDYLTATVLADIRMRELLEGGQLKEGTSSETKDGYVMDMTVTETLKERT
                     KGFTWQVFEVVLNVSWQQGVRKRSFSLKTFKTVRREVIKNENM"
     gene            complement(1548..2003)
                     /locus_tag="PKJ17_05735"
     CDS             complement(1548..2003)
                     /locus_tag="PKJ17_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD75507.1"
                     /translation="MKVNGNGFTLLELVIVMTLMVLFLGLSGVHFANRLPSAKLDGTA
                     RELSGLIRMARIEAMSIREERRVTIDLNRRTYGLKGAARRAVPEDVAISVVDPVRGAV
                     YGGEYDIIADASGTVGSCEIVLGTKKKTLRIEIDPVIGSVVVKGYGKEN"
     gene            complement(2000..>2963)
                     /locus_tag="PKJ17_05740"
     CDS             complement(2000..>2963)
                     /locus_tag="PKJ17_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOD75508.1"
                     /translation="PLDRSLNILAGVSEGRQMQEVVNSVLKLIREGGSFSDALKKHPR
                     VFPRLYVNMVRAGETGGVLDIVLEKLGEFLETAKELKDSVYSAMIYPVILGVTGSISI
                     VILLTFVIPRFSILFKEMGGTIPASTQMILYFSNFLRDLWWILLPAAVSGAFVVWRFV
                     KTERGGILWDTIKFRFLRDVVSKLETARFCRTMGTLIKSGVPFLQALQNSKDVIGNRV
                     VAAAIGAVARDVKEGKGIAVPLTEAKVFPQLALSMIKVGEETGQLDTMLLKVATTYEK
                     SLKDSVKRFMGLLEPAIILTMGLVIAFIVVSMLVAIFGILELPF"
BASE COUNT          639 a          900 c          768 g          656 t
ORIGIN      
        1 cgtcttcggc gccgttcaga cggtggagga cgtccttatc catccagtcg agagccgaat
       61 ccacgacggt gtccgccgtc tccttcggaa ctccgaggtt gacgagaaga ttgtcgagga
      121 tgatcttgtt cccatcgctc atggtattga gattgatccg ccccgcctcg ttgaagatcc
      181 tgacgacata cctgccatca ccgacatcgc cctcgatagc cgtgccgtcg accctcaagg
      241 tctctccgct gtcggggacg acggtcttgt tcaggttgtc ctgccggtag tatatctcca
      301 ggatcgcccg ctccagcccc gcctgcgcga gaaaaccttc tgccagtgaa tctcgaaaga
      361 acagggccgc cctgctttcg gtcttcgcaa gcacggaaaa ggacatgacg atgaccatga
      421 ggataatgat cacccacagc accatgatga gggcaaagcc atcttctttg caaagtaagt
      481 gtttcccttt tctcatgtcc cgctctcttc atcagggtca ttcccgggag gcttcctggt
      541 tcgcaggggt atcgtgagag agaacttcct ggcgcctttt tccaggtgga gctttatctt
      601 ttgcgggaga tgggtggtgt ccgccagttc gtccgaccag tcgccccctc ccagcccctt
      661 tatccccgcg gattgaaggt attcaaaccg gatgtcgtcg aaaccgacga ggagtttcgt
      721 ttccttcacg ttcttcatgc ctatggtggt ctccttcaga tagagcgcca gctttccctt
      781 ttcatccttt tcgatcctgt atgaggctat ggtatacccc ttccggccgc caaagagaga
      841 acggttggat gcgaatgtca tcgaatcgtg ggcacctatg aacaccgatc tcttgaggtc
      901 cttttgttcc ttcacggtga gggggaggca ggattgcaac tgggaatcga tgagtctcat
      961 ggaaaccctg aggcgctcca tccactccgc ctttctctcg cccccctcga gggagcggtg
     1021 cccggcacgc atcgaggcgg cgagaataac tacgatcatg gcgacaagtg cgatggagac
     1081 cacgagttcc aggagagtaa aaccccctga ccgcgaaagc ttgtccctac atattctcat
     1141 ttttgatcac ctctctcctc actgttttga aggtcttcag agagaatgac cttttcctca
     1201 ccccctgctg ccaggagaca ttgagcacca cctcgaacac ctgccacgtg aaacccttcg
     1261 tgcgctcttt gagcgtctcc gttacggtca tatccatcac atagccatcc ttcgtctcgc
     1321 ttgaggtgcc ctccttcaac tgcccaccct caagcagttc cctcatcctt atatcagcaa
     1381 gcacggtcgc cgtaaggtag tcgtcggaca tcgagagcgt cgtgagattc gccgaaaaca
     1441 actggaggac gacggtgacc acagttgcaa gaataacgaa ggccacaagg atctcgagga
     1501 gggtgaaacc ctccgggcct gcgggacaac ggcgccttcc ggatctttca gttttccttc
     1561 ccataaccct tcacgaccac ggacccgatc acaggatcga tctcgatcct gagggtcttc
     1621 tttttggtac cgagaacgat ctcgcatgac ccaaccgttc ccgacgcatc ggcgatgatg
     1681 tcgtattctc ctccatagac cgcgcccctg acagggtcca ccacgctgat ggcgacatcc
     1741 tcaggaaccg cgcggcgggc cgctcctttg aggccgtatg tcctgcggtt aaggtcgatc
     1801 gtcacgcgcc gctcttccct tatgctcatc gcctctatgc gcgccatgcg gataaggcct
     1861 gaaagctccc gcgccgtacc gtcaagcttc gctgagggaa gacggttcgc gaaatgcacg
     1921 cccgacaggc caagaaacag caccatgagg gtcatgacga tcaccagctc gagaagcgtg
     1981 aaaccattac cgttgacctt caaaagggca gctccaggat accgaatatc gcaacgagca
     2041 tggagactac aatgaaagcg atgacgagcc ccatggtaag gatgatagcg ggctcaagaa
     2101 gacccatgaa ccgcttgacc gagtctttca ggctcttctc gtaggttgtc gccaccttga
     2161 gcagcatggt atcgagctgc cccgtctctt cgcctacctt tatcatggac aacgccagct
     2221 gagggaagac cttcgcctcg gtgagcggca cggcaatgcc cttgccctcc ttcacgtccc
     2281 ttgccaccgc accgatcgcg gcagccacga cgcggttccc gatgacgtct ttggagttct
     2341 gcagggcctg gaggaaggga acgccgcttt ttatgagggt ccccatggtc ctgcagaatc
     2401 gggccgtttc gagcttgctc acgacgtccc tgaggaacct gaactttatc gtgtcccaaa
     2461 ggatcccgcc ccgctcggtc tttacaaacc gccatactac aaacgctccc gatacagccg
     2521 ccggaagaag gatccaccag aggtccctca gaaagttact gaagtaaagg atcatctgcg
     2581 tggacgcggg aatcgtcccg cccatctctt tgaagagaat ggagaagcga ggtatgacga
     2641 aggtaaggag gatgacgatc gaaatactcc ccgtgacgcc gaggatcacg gggtagatca
     2701 tggcggagta gacactgtct ttcagttctt tcgctgtttc gagaaactca cccagctttt
     2761 cgaggacgat atccagaaca ccgcccgttt caccggcccg gaccatattc acatagagcc
     2821 tgggaaaaac cctgggatgc ttcttcaggg catcggaaaa ggaaccccct tcccgtatga
     2881 gtttcagaac ggaattgacc acctcctgca tctgcctgcc ttcagagacc ccggccagga
     2941 tgttgaggct ccggtcgagg gga
//
LOCUS       DAOPOA010000230         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_36343_length_2961_cov_4.772540, whole genome shotgun sequence.
ACCESSION   DAOPOA010000230 DAOPOA010000000
VERSION     DAOPOA010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36343_length_2961_cov_4.772540"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKJ17_05745"
     CDS             complement(<1..383)
                     /locus_tag="PKJ17_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75509.1"
                     /translation="MQRGESQRREDREGAASHKGLPSRFVPFESDDVTVSSGRKPAPG
                     GAREQLPVHVPASGSPQTMEERRDGQDGVLTVKGPGSAVESGVIRYTVNVGPYDTSVS
                     TVAMRKYGEASSHRAWRPGTFEVTI"
     gene            612..1409
                     /gene="nudC"
                     /locus_tag="PKJ17_05750"
     CDS             612..1409
                     /gene="nudC"
                     /locus_tag="PKJ17_05750"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOD75510.1"
                     /translation="MNDDVSSHPVFLMRDHKLLVDLSSGVPRVPDRSVAEEFGLSFAD
                     GYRLGPLNGVDCYALSVTGEDEPSRGMDYLGLRKLFGVLDDTRYDMAVKALAILNWDR
                     TCRFCSACGSPLRRHETILAKVCEVCGSTMFPRISPAVIVLVERHEKVLLARASRFAE
                     NLYSVLAGFTEPGETLEDTVRREVREEVGVEVGDIRYFGSQPWPYPDSLMIGFTASWV
                     AGEIAADNDEITDARWFTADDLPKIPDRLSISRALIDWFIDKHRKGK"
     gene            1411..2379
                     /locus_tag="PKJ17_05755"
     CDS             1411..2379
                     /locus_tag="PKJ17_05755"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOD75511.1"
                     /translation="MQAIVFDGRLKLTGGRPRPVPARGEALIRVTMAGICNTDMEITK
                     GYLGFRGIMGHEFTGVVEAEGGRSGRLVGKRVVGEINCGCGACDLCLAGLEKHCPHRT
                     TIGILGRDGAFAEHMTLPVGNLYEIPEGLSDEEAVFTEPLAAAFEILEQVRIGTSDRV
                     LVLGDGKLGILCALVLRLTGARVTLAGKHPAKLGIARDQGVPVTLTKDLPAGGKHDVV
                     VEATGSAGAFETAMMLTRPRGVIVLKSTVAHGTPINLAPLVIDEITVVGSRCGPFKPA
                     LKALARGLVDVRPLISAVFPLEDAKKAFAAAGKRNTLKVLLDLRKG"
     gene            2443..>2961
                     /locus_tag="PKJ17_05760"
     CDS             2443..>2961
                     /locus_tag="PKJ17_05760"
                     /inference="COORDINATES: protein motif:HMM:NF021371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HOD75512.1"
                     /translation="MSKRRAPSVVMVLKDMLYVSYLLPAERIASMVPRGVVPATADGR
                     NGFLSLVIFKGNTKEAFHIPAPSIPFDQVNIRTYVVDPRTKETAVYFIRCGISGRFIT
                     FMYRMLSGMPVEHCAFNITADTGDTGNYDSYRVEGNWRGPFTITASETAPAVTGLDPF
                     PNVDRALLYLMDP"
BASE COUNT          579 a          910 c          889 g          583 t
ORIGIN      
        1 cagattgtca cttcaaaggt accggggcgc caggcgcggt gggagcttgc ctctccatat
       61 tttctcatcg ccaccgtgct cacactggta tcgtaaggac cgacatttac cgtgtaacgg
      121 atgacgccgc tctcaacggc ggagccggga cctttcaccg tcagcacccc gtcctgtcca
      181 tcccttctct cttccatggt ctgagggctt cccgaggcag ggacgtggac gggcagctgc
      241 tcccgcgctc ccccgggagc gggtttcctg ccggaactga ccgtcacatc atcgctctcg
      301 aagggaacaa agcgtgaggg caatcccttg tgtgaagccg cgccttcccg gtcctcccgg
      361 cgctggctct cgcctctttg catctcccgg attgtcggtg caaagatgcc ggttcgcaga
      421 ttctgggcgg ttatgagaga agtattcatt tgaatgtgtt ggtatcatat catttttttc
      481 ttttttcgac gagaaaaaaa tcgaaggtcc ggtacaacgg cctctagagc cttgccaggc
      541 cttcttcttg actttcattg tctcatgcag tattgattca tacactggca aacgaaggag
      601 atgaaccggc catgaacgat gatgtcagct cgcaccccgt cttcctcatg cgcgaccaca
      661 agctgcttgt cgacctgtca tcgggcgtgc cccgggtacc cgaccggtct gtcgctgagg
      721 aattcggatt gtcctttgcg gacggatacc gactcggtcc gctcaatggc gtcgattgct
      781 atgctctgtc cgttacaggc gaagacgaac cctcccgggg catggactat ctgggcctca
      841 gaaagctctt cggcgtcctc gacgacacca ggtatgacat ggccgtcaag gccctcgcca
      901 tactcaattg ggacaggaca tgccggttct gcagcgcctg cggttccccg ctccgacggc
      961 atgagaccat tctcgccaag gtgtgcgagg tctgcggttc aacgatgttc cccaggatat
     1021 ccccggcggt gatcgttctc gtggaaaggc acgagaaggt cctcctcgcc agggcgagca
     1081 gatttgccga aaacctctac agcgttctcg cgggttttac ggagcccggg gaaacactgg
     1141 aggacacggt gcgcagggag gtgagggaag aggtgggcgt ggaggtgggc gacatccgct
     1201 atttcggcag tcagccgtgg ccctaccccg attccctcat gataggcttc acggcctcct
     1261 gggttgccgg ggagatcgcc gcggacaatg atgagataac cgacgcgcgg tggttcaccg
     1321 cggacgacct tccgaaaata ccggacaggc tgagcatctc acgagcgctg atagactggt
     1381 tcatcgacaa acacaggaag gggaagtagg atgcaggcga tagtcttcga cggacgcctg
     1441 aagctgactg gcggcaggcc caggccggtc cccgcacgcg gtgaggctct gatcagggtc
     1501 acaatggcgg gcatctgcaa tacggacatg gagataacga aggggtatct cgggtttcgc
     1561 gggatcatgg gccacgagtt caccggcgtc gtcgaggctg agggcggcag gtcggggagg
     1621 ctcgtcggga agcgcgttgt cggagagata aactgcggct gcggcgcgtg cgacctttgc
     1681 ctcgcaggcc ttgaaaaaca ctgcccccac aggacgacca taggcatact gggaagggac
     1741 ggcgcgttcg ctgagcacat gacgcttccg gtgggaaacc tttatgagat ccccgaggga
     1801 ctgagcgacg aagaggcggt cttcacagaa ccgctggcag cggccttcga aatactcgaa
     1861 caggtgcgga tagggacatc cgacagggtg ctcgtcctcg gcgacggaaa actggggatc
     1921 ctctgcgccc tcgtgctcag gctcacgggg gcacgggtga cactggcagg aaagcacccc
     1981 gcaaagctcg ggatcgcccg ggaccagggc gtcccggtta cactgacaaa ggacctgccg
     2041 gcaggcggca agcacgacgt cgtcgtcgag gcgacgggca gcgccggggc cttcgagacc
     2101 gccatgatgc ttaccaggcc gcggggcgtc atcgtcctca agagcaccgt ggcccatgga
     2161 acccccatca accttgcccc cctcgtcata gacgagataa ccgtcgtcgg gtcccgctgc
     2221 ggtcccttca aacccgccct gaaggcactc gcccggggcc ttgtcgatgt caggcccctc
     2281 atctcggccg tgtttcccct ggaggatgcg aaaaaggctt tcgcggcggc aggaaagcgc
     2341 aacaccctca aggtcctcct cgatctcagg aagggatagg gcgccggtcc tctcttcctt
     2401 ttcgttgcca taaaccgatc ctgacggtat agtatcattg tcatgagcaa gaggcgcgct
     2461 ccttccgtcg tgatggtcct caaggacatg ctttatgtct cctacctcct gcccgcggaa
     2521 cggatagcgt ccatggtccc ccgcggtgtc gtgccggcga ccgcagacgg caggaacggc
     2581 ttcctttccc tcgtcatctt caaaggcaac acgaaggagg cgtttcacat ccctgccccg
     2641 tccatcccat tcgaccaggt caacataagg acctatgtgg tcgacccgcg gacgaaggaa
     2701 acggccgtct atttcatcag atgcggtatc agcggccgct tcatcacctt catgtaccgg
     2761 atgctctcgg ggatgcccgt cgaacactgc gccttcaaca tcaccgcgga taccggtgac
     2821 acggggaatt acgacagcta tcgcgtggag gggaactggc gcgggccgtt cacgatcacg
     2881 gcctccgaga ccgcccccgc agtgaccggc ctcgatccct tcccgaatgt cgacagggcc
     2941 ctcctttacc tcatggaccc c
//
LOCUS       DAOPOA010000231         2958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_36396_length_2958_cov_5.032380, whole genome shotgun sequence.
ACCESSION   DAOPOA010000231 DAOPOA010000000
VERSION     DAOPOA010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36396_length_2958_cov_5.032380"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..823)
                     /locus_tag="PKJ17_05765"
     CDS             complement(71..823)
                     /locus_tag="PKJ17_05765"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOD75513.1"
                     /translation="MIRTSLVKSKTVSRVLRSILVLFALLLIFLVFYAYQEGAWREIV
                     AYYRFFFDYKKLRLFILSFGPFAALAFIVIQSLQVVFAPVPGEITGFVGGLIFGNAWG
                     TILSTIGLTLGSLGAFAISRMLGLRFVEKVVKKTYIDKFNFFITHKGLNVSFILLLIP
                     GFPKDSLCYLLGLTRIRLLDFIIMNVFGRLPGTLMLTMQGTAVYSQQYRLFFVLLSLS
                     IVLTFVLYLARGFLVRKIVRAVRRTRVFLRRK"
     gene            complement(820..2835)
                     /locus_tag="PKJ17_05770"
     CDS             complement(820..2835)
                     /locus_tag="PKJ17_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOD75514.1"
                     /translation="MSAAMDRVSSLKNTELFRALEDGDLEKLSTKLRERVYPPNTAIV
                     REGASGDAMFIIKNGQVEVRKREQNLGVDLTIATLGMGACFGEMALLTGKPRSATVMA
                     IAATELFVLEKKDFDALLLEHPSISISINKIVAERIEEMNTQKSMGVVSMQTLKIDPD
                     FITVIPEAEALKYKMLATSYGNGSLTIAMVNPQDILALDEARRLIKAKFTKSVALDQV
                     IVTEEDFKQFMEGEYKTITKPLVEEKPEVNLDDFLDSMDHIQSDIMKDVEFDEGQEDD
                     SGITDLAREAEGAPIIRLTNNIIAMALKKGASDIHIEPMERGLRVRYRIDGVLREEMM
                     LPKKVQLPLISRVKIISKLDITERRLPQDGRITIKLGSKSVDFRVSTVPTKFGEKICT
                     RILDKTNTTMGLDKLISHTPVLELVRDMIKKPYGIIYVTGPTGSGKSTTLYSSLAEIN
                     DIGVNISTVEDPVEYDLEGVNQVQVNSDIGLDFARVLRAFLRQDPDIILVGETRDTET
                     AKIAVEAALTGHLVFTTLHANDAPSTFMRLSEMGIEPFLISTSVIGIVAQRLVRRICG
                     KCKEAYQADPMILKYLGLDENTTLYKGKGCDVCGGTGYKGRVGVYEVLMVNEELRHLI
                     AEGADTLAIRDAAIRSGMKALKEYCLILLGEGHTTVDEVLRTVAVQN"
BASE COUNT          616 a          793 c          862 g          687 t
ORIGIN      
        1 ttatttttta ttgtatatta ataaaacatt ctgtcaagaa ggaatgtgaa aaaaacccat
       61 ttatttcaga ttacttccgg cgaagaaaaa cgcgtgtcct gcggactgcc ctgacgatct
      121 tcctgacgag gaaacctctg gccagataaa gaacgaaggt aaggacaata ctcagggaaa
      181 gaagaacgaa gaacaggcgg tattgctggg agtatacggc ggtgccctgc atggtcagca
      241 tcagcgttcc aggcagtctt ccgaagacat tcattatgat gaagtcgaga agcctgatgc
      301 gggtcaatcc cagcagatag catagcgagt cctttggaaa cccggggatg aggaggagaa
      361 tgaaggagac attgagccct ttgtgtgtga tgaagaaatt gaacttgtca atgtaggtct
      421 tcttcacgac cttctccacg aatcgcaatc cgagcatcct cgatattgcg aatgccccga
      481 gagacccgag ggtgaggccg atcgttgaga ggatcgttcc ccaggcgttg ccgaagatca
      541 ggccgccgac aaatcctgtt atctcaccgg gaaccggcgc gaaaacgacc tggagggatt
      601 ggatcacaat gaaggcgagt gcggcgaagg gcccgaagga gaggatgaag agccgcagct
      661 tcttgtagtc gaagaagaaa cggtaatagg cgactatctc tctccacgca ccttcctgat
      721 aggcatagaa aacgagaaag atgaggagaa gcgcgaagag cacaaggatg gaacgcagaa
      781 cgcgagagac ggtctttgat ttgacgagtg aggttcggat caattttgaa ccgcgactgt
      841 ccgcagcact tcgtcaacag ttgtgtgccc ttcaccgaga agaatgagac agtattcctt
      901 caaggccttc atgccgcttc tgatcgccgc gtcccggatg gccagggtgt cggccccttc
      961 cgcgatgagg tgtctgagct cctcgttgac catgaggacc tcgtataccc cgacccgtcc
     1021 cttgtatcct gttcccccgc acacgtcgca tccttttccc ttatacagcg tggtgttctc
     1081 gtcgagaccg aggtacttca ggatcatggg gtcagcctgg taggcttcct tgcacttgcc
     1141 gcagatcctg cgcacgagac gctgggcgac gatgccgatg acggaggtgg agatgagaaa
     1201 tggttcgatg cccatttcgg acagacgcat gaaggtgctt ggagcgtcgt tcgcatggag
     1261 tgtcgtgaaa acgaggtggc ctgtgagcgc cgcctccacg gcgatcttcg cggtctccgt
     1321 gtcacgcgtc tcgccgacga ggatgatgtc cgggtcctgc ctgaggaagg ccctcaggac
     1381 ccgcgcgaag tcgagcccga tgtcggagtt cacctgcacc tggttgacgc cctcgagatc
     1441 gtactcgacg gggtcttcga cggtggatat gttgacgcct atgtcgttga tctcggcgag
     1501 cgaactgtaa agcgtcgtgc tctttccaga ccccgtcggg cccgtgacgt agatgatgcc
     1561 gtagggcttc ttgatcatgt cgcggacgag ttcgagaacg ggcgtgtggg agatgagctt
     1621 gtcgaggccc attgtcgtgt tcgtcttgtc gaggatcctt gtacagatct tctctccgaa
     1681 cttcgtcggc acggtggaga cacggaaatc gacggacttg ctgccgagtt tgatcgtgat
     1741 cctgccgtcc tggggaagcc gcctctctgt gatgtcgagt ttcgatatga tcttcaccct
     1801 gctgatgaga ggcaactgta ccttcttcgg gagcatcatc tcctcgcgca gcacgccgtc
     1861 gatgcggtac cgcacgcgca gcccgcgctc catgggttcg atgtggatat cgctggcacc
     1921 ctttttcagg gccatggcga tgatgttgtt cgtcagcctg atgatggggg cgccctcggc
     1981 ctcgcgcgcg agatcggtga tacccgagtc gtcctcctgg ccttcgtcga actcgacatc
     2041 cttcattatg tccgattgga tgtggtccat ggaatccagg aaatcgtcga ggttgacctc
     2101 cggcttttcc tcgacgaggg gcttcgtgat ggtcttgtac tcgccctcca tgaactgttt
     2161 gaagtcttcc tcggtgacga tgacctgatc gagggcgacg ctcttcgtga acttcgcctt
     2221 gatcagcctg cgcgcctcat cgagggcgag tatgtcctgg ggattgacca tggcgatggt
     2281 gagactcccg ttgccgtacg aggtggccag catcttgtac ttcaatgcct ccgcctcggg
     2341 tatgacggtg atgaaatcgg gatcgatctt gagcgtctgc atggagacga cgcccatgct
     2401 cttctgcgtg ttcatctcct cgatgcgttc cgcgactatc ttgttgatgg atatggagat
     2461 gctgggatgc tcgaggagca gcgcgtcgaa atccttcttc tcgaggacaa aaagctccgt
     2521 cgccgcgatc gccatgacag tggccgagcg gggcttgccg gtcaagagcg ccatctcgcc
     2581 gaagcacgcc cccatgccga gcgtcgctat ggtgagatcg acaccgaggt tctgctccct
     2641 tttccttacc tcgacctgtc cattcttgat gatgaacatc gcgtcgccgg aggcgccttc
     2701 acggacgatg gccgtgttcg gaggatagac cctttcccga agtttcgtcg aaagcttttc
     2761 cagatcacca tcctcaaggg cacggaacag ttccgtgttc ttgagcgacg aaaccctgtc
     2821 cattgcggcg ctcactacga catcctccag ggtgttgggc cccgatcgtt catctgtccc
     2881 ttaagctcca ccggggtagt taagtctttg aggccggctt gaggccggcc tgctgaaaat
     2941 tataaaggat attctcaa
//
LOCUS       DAOPOA010000232         2958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_36404_length_2958_cov_3.411643, whole genome shotgun sequence.
ACCESSION   DAOPOA010000232 DAOPOA010000000
VERSION     DAOPOA010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36404_length_2958_cov_3.411643"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1700
                     /gene="nrdD"
                     /locus_tag="PKJ17_05775"
     CDS             <1..1700
                     /gene="nrdD"
                     /locus_tag="PKJ17_05775"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOD75515.1"
                     /translation="SVFSQEVADAHMNGDIHLHDLGFVDRPYCSGQSLEYIKKFGLDL
                     PHSLSMARPAKHAEVLLAHLVKFAAALQSHFAGAIGWDAINIFFAPYIEGMSDNDVKQ
                     LAQMLIFEFSQQAVARGGQAIFTDINIYWEIPKHFVNVPAIGPNGKFTGKTYGEYEKE
                     AQRFAWKLFEVYKEGDGAGRPFFFPKPIVHITEKFFHTDGHMDFLNLICDVASDKGNT
                     YYVFDRGETAKISECCRLSFKLEESDLLDAKEPWRMRYCALQNVSLNLPRLAYMAKND
                     DTKLFNLITERFILAVKAHKEKRAFMEKLLSLGENGPLALLTMNRDGMPYLRFNLASH
                     LIGMVGLNEMVQIHTGEEMHESRRALKFGIKAIAHMNLLTEKMRRQENMKIVLEQTPA
                     ESTSYRFARLDLRYFSPHAGRIMKGDIASGEVYYTNSTHLNVKHAMNPIERVTTEGLF
                     HPLIEAGSISHVWLGEAQPSKEAIADFVIKTFKHTTNDQIAFSPEFTTCNKCHRTGRG
                     LADTCSYCGSTDVDGITRITGYFTKVSSWNKGKLGELKDRYRNSEFFSERRKAAAND"
     gene            1737..2000
                     /locus_tag="PKJ17_05780"
     CDS             1737..2000
                     /locus_tag="PKJ17_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOD75516.1"
                     /translation="MGIVKIFYKDDCPMCPMAKQLKDHLREKNVAVDDYNVGTTEGLA
                     EATFYRVMALPTILVEDESENSLGEWRGNVPHIDEVLSAVQGA"
     gene            1981..2760
                     /locus_tag="PKJ17_05785"
     CDS             1981..2760
                     /locus_tag="PKJ17_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOD75517.1"
                     /translation="MQFKEPDCPAVKGFIETSFVDWKGCLASVLFLGGCNFRCPFCHN
                     RDLVLNPGGLEDVPIEYIVATIRKYRAQWVDRIVVTGGEPTIHKNLYRLMGLLKSEGM
                     KIKLDTNGSSPSAVKGLVNDGLVDAIAMDVKGPLDRYSRWCGINVDDRKVQESIEFIM
                     GSGIDHYFRMTVVPFLHREEDVYMVAAHLQGAKDFVVQTFRPDITLNTAFSRIRPFSP
                     DRMNQIKANVSDIMAGESVRGKEVRPHLSIAGKTDQDNLYS"
BASE COUNT          702 a          811 c          830 g          615 t
ORIGIN      
        1 tgtccgtctt ctcccaggag gtggccgatg cccacatgaa cggggacata cacctccacg
       61 acctcggttt tgtcgacagg ccatactgca gcggccagtc cctcgaatat atcaaaaagt
      121 tcgggctcga tctgccgcac tccctgtcca tggccaggcc ggctaagcat gcggaagtgc
      181 tccttgccca tctcgtcaag tttgcggcgg ccctccagag tcatttcgcg ggcgccatag
      241 gctgggacgc cataaacatc ttcttcgcgc cctatatcga ggggatgagc gacaacgacg
      301 tgaagcagct cgcgcagatg cttatcttcg aattctccca gcaggccgtg gctaggggcg
      361 gccaggcgat cttcaccgac ataaacatct actgggagat accgaagcac tttgtcaacg
      421 tgccggccat cggccccaac ggcaagttta cgggcaagac ctacggtgaa tatgagaagg
      481 aagcccagcg ctttgcctgg aagctcttcg aggtctacaa ggaaggcgac ggggcggggc
      541 ggcccttctt tttcccgaaa cccatcgtcc atatcacgga gaagttcttc cataccgacg
      601 gccacatgga tttcctgaac ctcatatgcg acgtcgcatc cgacaagggc aacacctact
      661 atgtcttcga caggggagag acggccaaga tatccgaatg ctgccgcctg agcttcaagc
      721 tcgaggaaag tgaccttctt gacgcgaagg agccgtggag gatgcggtac tgcgccctcc
      781 agaacgtgtc cctcaacctg ccgaggcttg cctacatggc gaagaacgac gacacgaagc
      841 ttttcaacct catcacggag aggttcatcc ttgccgtcaa ggcccacaag gagaaacggg
      901 cgttcatgga gaaacttctt tccctggggg agaacggacc gctcgcgctc ctcacgatga
      961 accgggacgg gatgccctat ctcaggttca atctcgcatc gcacctcata ggcatggtcg
     1021 gtctcaacga gatggtgcag atacacaccg gcgaggagat gcacgaatca agacgggcgt
     1081 tgaagttcgg catcaaggcc attgcccaca tgaaccttct cacggagaag atgcggcgac
     1141 aggagaacat gaagatcgtc ctggagcaga ccccggcgga aagcacgagt taccggttcg
     1201 cgcgcctgga cctccggtat ttctctcccc acgccggcag gatcatgaaa ggcgacatcg
     1261 catccggtga ggtctattac acgaattcga cgcatctgaa cgtcaagcac gccatgaatc
     1321 ccatcgaaag ggtaaccacg gaggggctgt tccatcccct tatcgaggca ggctccatat
     1381 cgcacgtctg gcttggagaa gcgcagccct cgaaggaggc catcgccgac ttcgtcatca
     1441 agaccttcaa gcacacgacc aatgaccaga tagctttctc cccggaattc accacatgta
     1501 acaagtgcca caggacgggg cggggtcttg ccgatacctg ttcgtactgc ggttccaccg
     1561 atgtggatgg tattacgagg ataaccggat acttcaccaa ggtatcaagc tggaacaaag
     1621 gtaagctggg agagctaaag gaccgttatc gcaattcgga gttcttctcc gaaaggcgca
     1681 aggcggctgc caacgactag aacactgacg gatcctacaa aattcaagga gggagtatgg
     1741 ggatagtgaa gatcttctac aaggatgatt gcccgatgtg tccaatggcg aaacagctga
     1801 aggaccatct ccgggaaaag aacgttgccg ttgacgacta caacgtcgga acgactgaag
     1861 gccttgccga ggcgacattc tatcgcgtca tggcattgcc caccatcctc gttgaagatg
     1921 agtcggagaa ctccctgggc gagtggagag gcaacgtgcc tcatatcgac gaggtactca
     1981 gtgcagttca aggagcctga ttgcccggct gtaaagggtt tcattgagac cagttttgtg
     2041 gactggaagg ggtgtctcgc atccgttctc ttccttggcg gctgcaactt ccggtgtccc
     2101 ttctgtcaca accgcgacct cgttctcaac ccgggggggc tggaggacgt cccgatcgag
     2161 tatattgtcg caaccataag gaaatacagg gctcagtggg tcgacaggat cgtggtgacc
     2221 gggggtgagc cgacaataca caagaacctg taccggctta tgggtctctt gaaaagcgaa
     2281 gggatgaaga tcaagctcga tacgaacggg tcaagcccct ctgccgtgaa gggccttgtc
     2341 aatgacggcc tcgtggacgc gatcgccatg gatgtcaaag gtcccctgga ccggtattcc
     2401 cggtggtgcg gtatcaatgt cgatgaccgc aaagtgcagg aaagtattga gtttatcatg
     2461 ggttccggga tcgatcatta cttccgcatg acagttgtgc cttttctcca cagggaggag
     2521 gatgtctaca tggttgctgc ccatcttcag ggggcgaaag acttcgtggt ccagactttc
     2581 aggcccgata tcactctgaa taccgccttt tcgcggataa ggcctttcag ccctgacagg
     2641 atgaaccaga tcaaggcgaa cgtctcggac attatggccg gggagagtgt ccggggaaag
     2701 gaagtgaggc cgcatctcag cattgccggc aagacggacc aggacaacct gtatagttga
     2761 aagcagagaa ccgggggatc gatgcccggc atgtccgtgg atgacagaaa gagtaaatga
     2821 aaaggcaaaa gattcgatct tttgccttta ttttctatcc ggcctaatta tttccaggtc
     2881 tcttccttgg atcctgctct ctgttcccca cctttcctat tccaggaaac tttccagtct
     2941 cttccgccgt gaggggtg
//
LOCUS       DAOPOA010000233         2956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_36441_length_2956_cov_3.920028, whole genome shotgun sequence.
ACCESSION   DAOPOA010000233 DAOPOA010000000
VERSION     DAOPOA010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36441_length_2956_cov_3.920028"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="PKJ17_05790"
     CDS             complement(<1..1264)
                     /locus_tag="PKJ17_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOD75518.1"
                     /translation="MRFFENIRIGKRLGIGFAIFIVIIALLALLGFKIIDDVDRKAGH
                     IVEAALEKTILANTVMGAIYNAYNSMGVIAFATDAAVIEAEKENLGGMRQTYGEALAK
                     LEKLEQDPKGKDLIARFKQTTAKAKDSNNRIIEYAGGKEQARAIETYINENKPATKEI
                     ITTLQSIIRYQEDNVRGQFASIREANRKYTLILLVFGIAAVVLGIILSVTITRSIVVP
                     IRGNTRAVKAMAKGNLAFEVPTDRKDEFGDEMKAAKEMVEKWREIVGSLKSAAASISS
                     AGVQLSASAEQMSKGSGEQANRSSQVAAASVEMSQTVVDIARNTNNIANSAANTVKIA
                     KEGEEIVNQATKEVEEIARTVGDSAESIRTLGERSNQIGEIVNVIDDIADQTNLLALN
                     AAIEAARAGEQGRGFAVVADEVRKLAERT"
     gene            complement(1373..2386)
                     /gene="amrS"
                     /locus_tag="PKJ17_05795"
     CDS             complement(1373..2386)
                     /gene="amrS"
                     /locus_tag="PKJ17_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOD75519.1"
                     /translation="MKEASFYEKRDGKTIHCFLCRHNCVIADGKRGLCGVRENRDGTL
                     YSLVWGLPCAYHVDPIEKKPLFHFYPGSKAFSVATVGCNFRCLHCQNHDISQGPRENG
                     RVFGETVSPGEIVEQALAARCASISYTYTEPTIFFEYAFDIAREAREKGMANNFVTNG
                     YIEEAPLRAIAPYLDAANIDLKGFSEVFYRDVCGARLSGVLDSIRLYRELGVWIELTT
                     LIIPGHNDSDEELRSIARFIAKDLGVEVPWHVSAFYPTYKLLDVEPTSPQSLARAREI
                     GRDEGLRYVYEGNIPGSGGENTSCHNCGKTVIGRYGYSITEYNLKGGACKFCGTVLDG
                     RGF"
     gene            complement(2390..>2956)
                     /locus_tag="PKJ17_05800"
     CDS             complement(2390..>2956)
                     /locus_tag="PKJ17_05800"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOD75520.1"
                     /translation="HHLAHVAREGQFLLALAEFGGDVLYVEASCRKGKKELTLTGNMG
                     EVMKESVQAALTYIKSKAEALGIDKAIFDDLEMHVHVPQGAIPKDGPSAGITMAVAMI
                     SAITGRPINRKIAMTGEITLTGRVLPIGGLKEKTLAALRNRMKRVLIPHENDHELVEI
                     PQYVKKKITFLSVKTMDDVVAAVFGKKV"
BASE COUNT          492 a          906 c          866 g          692 t
ORIGIN      
        1 cggtgcgctc cgccagcttg cgcacctcgt cggccacgac cgcgaaaccc ctgccctgct
       61 cgccggcccg ggcggcctcg atcgcggcgt tgagcgccag gaggttcgtc tggtcggcaa
      121 tgtcatcgat gacgttgacg atctctccga tctggttcga acgctccccg agggtccgta
      181 tcgattcggc ggaatcgccg acggtccggg ctatctcctc cacttccttc gtggcctggt
      241 tgactatctc ttcgccttcc ttcgctatct tcaccgtatt cgccgccgag ttcgctatgt
      301 tgttcgtgtt gcgcgctatg tcgactaccg tctgtgacat ctccacggag gccgcggcca
      361 cctgggacga ccggttggcc tgctccccgg agcccttcga catctgctcc gcgctggcgc
      421 tgagctggac accggcggag gatatgctcg ccgcggccga tttgaggctc ccgacgatct
      481 cgcgccactt ctccaccatt tccttcgccg ccttcatctc gtcgccgaac tcgtccttcc
      541 tgtccgtggg gacctcaaag gcgaggttcc ctttcgccat ggccttcacc gcccgggtgt
      601 tcccgcggat gggcacgacg atgctccggg tgatcgtgac ggaaaggatg atgccgagca
      661 ccacggcggc tatgccgaag acgaggagga tcagggtgta cttcctgttg gcctcgcgga
      721 tggacgcgaa ctggccgcgg acattgtcct cctggtaccg gatgatggac tgcagggtcg
      781 tgatgatctc ctttgtggca ggtttgttct cgttgatata tgtctctatc gcccttgcct
      841 gttccttgcc ccccgcgtac tcgatgatcc ggttgttgga gtccttcgcc ttcgctgtcg
      901 tctgtttgaa gcgggcgatg aggtcctttc ccttggggtc ctgctcgagt ttttcgagct
      961 tcgccagggc ctcgccatag gtctgccgca tcccgcccag gttctccttt tccgcctcga
     1021 tgacggcggc gtccgtggca aaagcgatga cacccatgga attgtaggcg ttgtagatcg
     1081 cccccattac ggtgttggcg aggatggtct tctccaaggc cgcctcgacg atatggccgg
     1141 ccttcctgtc cacgtcatcg atgatcttga aacccagaag ggccaagagc gctatgatga
     1201 cgatgaagat ggcgaaaccg atgccgagac gtttgcctat cctgatattc tcaaagaaac
     1261 gcatgtctcc cccttcgttg cttcatttgt ttaaggatta ggtattctat atttaattaa
     1321 gggagtgact ttatttcaag acatttcttc gccgggggcc ttgcccggcg ggtcagaacc
     1381 ccctgccgtc gagaacggtg ccgcagaatt tacaggcccc gcccttcagg ttgtactccg
     1441 tgatcgagta cccgtaacgc cctatgaccg tcttgccgca gttgtggcag gaggtgttct
     1501 ctcccccgga gccggggatg ttgccttcgt agacataacg gagcccctca tcgcggccta
     1561 tctcccgggc ccgggcgagg ctctgcgggc tcgtcggctc cacgtcgagg agcttgtagg
     1621 tgggatagaa ggcgctcacg tgccagggca cctccacgcc gaggtctttc gctatgaacc
     1681 gcgcgatgga cctcagttcc tcatcgctgt cattgtgccc cggtatgatg agcgtcgtca
     1741 gctctatcca gacccccagt tccctgtaaa ggcgtatcga atcgaggaca ccggaaagcc
     1801 gagccccgca tacgtccctg taaaagacct cgctgaagcc tttcaagtcg atgttcgccg
     1861 cgtcgagata gggggcgatg gcccgaagcg gcgcctcctc gatgtacccg ttggtcacga
     1921 agttgttcgc catgcccttt tcccgggctt cgcgtgctat gtcgaaggcg tattcgaaga
     1981 agatggtcgg ttcggtgtag gtgtaggaga tgctcgcgca ccgtgccgcg agcgcctgct
     2041 ccacgatctc cccggggctc accgtctctc cgaagaccct cccgttctcg cggggaccct
     2101 gggagatatc atggttctgg cagtggaggc agcggaagtt gcatcccacc gtggccactg
     2161 agaaggcctt cgagccggga tagaagtgaa agaggggctt cttttctatg ggatccacgt
     2221 ggtaggcaca gggaaggccc cagacaaggc tgtagagggt gccatccctg ttctcgcgga
     2281 caccgcacag gccccttttg ccgtcggcaa tgacgcagtt gtggcggcac agaaaacaat
     2341 ggatggtctt accgtccctc ttctcataaa aggatgcttc tttcatgttt cacacctttt
     2401 ttccaaaaac tgccgccacc acgtcgtcca tggtcttcac ggaaaggaag gtgatcttct
     2461 tcttcacgta ctggggtatc tcgacaagct cgtggtcgtt ctcgtggggg atgaggaccc
     2521 ttttcatccg gttgcgcagg gcggcgaggg tcttttcctt gagaccacct atgggaagga
     2581 ccctgcccgt cagggtgatc tcccccgtca tcgctatctt cctgttgatg ggacgtcccg
     2641 tgatggcgct tatcatggca acggccatgg tgatgcccgc cgagggaccg tctttgggga
     2701 tggcgccctg gggcacgtgg acgtgcatct ccagatcgtc gaagatggcc ttatcgatgc
     2761 cgagagcctc cgccttcgat ttgatatagg tgagcgccgc ctgcaccgat tccttcatca
     2821 cctcgcccat gttgcccgtg agggtcagtt ccttcttgcc cttgcggcag gaggcctcga
     2881 cgtagagcac gtcgccgccg aactccgcaa gggcaagaag gaactgaccc tcacgggcaa
     2941 catgggcgag gtgatg
//
LOCUS       DAOPOA010000234         2947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_36572_length_2947_cov_3.052213, whole genome shotgun sequence.
ACCESSION   DAOPOA010000234 DAOPOA010000000
VERSION     DAOPOA010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36572_length_2947_cov_3.052213"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PKJ17_05805"
     CDS             <1..1113
                     /locus_tag="PKJ17_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOD75521.1"
                     /translation="VGIVTDGTRVLGLGDIGPMAGLPVMEGKALLFKYLGGVDAFPIC
                     LDTKDPDEIVRTVKLIAPVFGGINLEDIENPKCFYVLEKLRAESPIPVWHDDQQGTAA
                     VTLAGLVNALKIVGKKIEDAKITMIGIGAANVCIVRMLVKAGADPRKIIVVDSKGILN
                     RKRDDIKPNYKEKLELAATTNGENREGDTAAALKGQDVLIALSKSGPDVIQKEWVASM
                     ADNAIVFVCANPIPEMWPWDAKEAGARIVATGRSDFPNQVNNSVGFPAIFRGTLDVRA
                     RTITDEMCIAAAYELARCAEDRGLTEDHLLPTMNEWEVFPREAVAVAKMAMKQGVARL
                     TIDEKELYNMAVTKIRRAQDEVGLLMEKGIIAPYAG"
     gene            1123..1962
                     /locus_tag="PKJ17_05810"
     CDS             1123..1962
                     /locus_tag="PKJ17_05810"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOD75522.1"
                     /translation="MREIHVDDIVSVVERLFIDANISLPEAMYGAIRDAIEKESSPVG
                     KEVLRELLRNADTAREEHMPICQDTGLAVTFLEIGQDAHVVGGDLTEAVTEGVRRAYR
                     NGYLRKSCCHPFSRKNTGDNTPPIIHTKIVPGDGMKITVLPKGGGSENYGEVRMLMPA
                     EGKEGVKAFVLEMVRKGGPNPCPPITVGVGIGGNFETSALLSKEALMVPVGTRNDDEE
                     LAAMERELLEEINATGIGPQGYGGTVTALDVHIKMAPCHLASLPVAVNIQCHAHRIGE
                     ALL"
     gene            1959..2516
                     /locus_tag="PKJ17_05815"
     CDS             1959..2516
                     /locus_tag="PKJ17_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOD75523.1"
                     /translation="MTTDLRRITTPLTDDVTGSLTAGEKVLLSGFIHTARDAAHKRFI
                     ETLDAGGDLPFDIRGQIIYYCGPAPAPPGKVIGSCGPTTSSRMDVYAPRLIAMGLRGM
                     IGKGKRSEGVKGALREFRAVYFGATGGAGALLARAVVAADVIAYEDLGPEAVVRLEVR
                     DMPLFVINDVHGNDLYLEGIERYRK"
     gene            2578..>2947
                     /locus_tag="PKJ17_05820"
     CDS             2578..>2947
                     /locus_tag="PKJ17_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOD75524.1"
                     /translation="MPEIRVAIAGVGNCASSLVQGIVYYSRKSEDAIGLMHSDICGYK
                     PGDIRIVAAFDIDRRKVGVPLEDAIFAPPNCTKTIEADIPPTGVTVLMGHVFDGVAPH
                     MEQYPPERSFVVADEEPVDVV"
BASE COUNT          645 a          826 c          949 g          527 t
ORIGIN      
        1 gtgggcatag tgaccgacgg aacgcgggtg ctcggtctcg gtgatatcgg tcccatggcg
       61 ggactgcctg tcatggaggg caaggcgctg ctcttcaagt acctgggtgg tgtcgatgct
      121 ttccccattt gtctcgatac gaaggacccc gatgagatcg tgcggacggt gaagctcatc
      181 gcccctgtct ttgggggcat caatctcgag gacatcgaga acccgaaatg cttctacgtc
      241 cttgagaagc tgagggcgga atcaccgatc cccgtctggc acgacgacca gcagggcacc
      301 gcggcggtca cccttgccgg tctcgtgaac gccctgaaga tcgtgggaaa gaagatcgag
      361 gacgcgaaga tcaccatgat cggcataggc gccgcaaacg tctgcatcgt gcgcatgctc
      421 gtcaaggcag gtgcggatcc caggaagatc atcgtcgtgg acagcaaggg aatactcaac
      481 cggaaacggg acgatatcaa gcccaactac aaggaaaaac tggagctcgc cgcgacaacg
      541 aacggagaga accgggaagg cgacaccgcg gcggcgctca aaggacagga tgttcttatc
      601 gccctgtcga agtcgggacc ggacgttata cagaaggaat gggtggcgtc gatggcagac
      661 aacgcgatag ttttcgtctg cgccaaccca atccccgaga tgtggccctg ggacgcgaaa
      721 gaggcggggg cccgcatagt tgccacgggc aggagcgatt tcccaaatca ggtgaacaat
      781 tccgtcgggt ttccggcgat attccggggc accctcgacg tcagggcccg gaccatcacc
      841 gacgagatgt gcatagccgc ggcctacgaa ctggcccggt gcgccgagga cagggggctc
      901 accgaagatc acctccttcc gacgatgaat gagtgggagg tcttccccag ggaagctgtg
      961 gccgtcgcga agatggcaat gaaacagggc gtcgctcgcc ttaccatcga cgaaaaggaa
     1021 ctctacaaca tggcggtcac gaagatccgc agggcccagg acgaggtggg cctgctcatg
     1081 gagaagggca tcatagcgcc ctacgccgga taaggagccc ccatgcgcga gatccacgtg
     1141 gacgatatcg tttccgttgt cgaacgtttg ttcatcgacg ccaacatcag tcttcccgag
     1201 gccatgtacg gtgccatccg ggacgcgatc gagaaggaaa gctctcccgt cggcaaggag
     1261 gtgttgaggg aactcttgag gaacgccgac acggcccggg aggaacacat gcccatatgc
     1321 caggacacag gtttggcggt gacctttctc gagatcggcc aggacgcgca cgttgtggga
     1381 ggagacctca ccgaggccgt gacggagggc gtccgcaggg cgtaccgcaa cggatatctc
     1441 agaaaatcct gctgccatcc tttcagcagg aagaataccg gggataatac gcctcccatc
     1501 atccacacga agatcgtgcc cggggatggg atgaagatca ctgttctgcc gaagggcgga
     1561 ggaagcgaga actacgggga ggtccgcatg ctcatgcccg ctgagggcaa ggaaggggtc
     1621 aaggcctttg tcctcgagat ggtgcgcaag ggaggcccca acccctgtcc gccgatcacc
     1681 gtcggtgtgg gcatcggggg caatttcgag acctcggctc tcctgtcgaa ggaggccctc
     1741 atggttcccg tggggacgcg gaacgatgac gaggaactgg cagccatgga gagggaattg
     1801 ctcgaggaga tcaacgcgac cggcatagga cctcaaggat acgggggcac ggtgacggcc
     1861 ctcgacgttc acataaagat ggcgccctgc catctcgcgt cgctacccgt ggcggtcaac
     1921 atccagtgcc acgcgcaccg catcggggag gcccttctat gacgacggac ctcaggcgga
     1981 taacgacacc tcttaccgat gacgtgacgg ggtccctcac ggcgggagag aaggttcttc
     2041 tctccggttt catccatacc gcccgggatg ccgcgcacaa gagattcatt gaaaccctcg
     2101 atgccggcgg ggacctgcct tttgacatca ggggacagat catctactat tgcggtccgg
     2161 cgccggcacc gccgggaaag gtcatcgggt cctgcggacc cacgacgagt tcccgcatgg
     2221 acgtctacgc gccgaggctc atcgccatgg gcctcagggg catgataggg aagggtaaac
     2281 gttccgaagg ggtcaaggga gcgctcagag agttcagagc ggtctatttt ggggcgacag
     2341 gaggggccgg cgcgctgctc gcgcgcgccg ttgtcgccgc ggacgtgatc gcctatgaag
     2401 accttggtcc tgaggcggta gtcaggctcg aggtgaggga catgccgctt tttgtcatca
     2461 acgacgttca tggcaacgac ctgtacctgg aaggcattga acgctacagg aagtgatgtc
     2521 cctgctttga ctttttgggg taagtattct aaaagaatat taagagaagg agatgcaatg
     2581 ccagagattc gtgtcgccat tgccggcgtg gggaactgtg cgagttcgtt ggtacagggt
     2641 atcgtctact attcccggaa gagcgaagat gccatcggac ttatgcacag cgatatctgc
     2701 ggttacaagc cgggagacat ccggatcgtg gcagccttcg acattgaccg gcggaaggtg
     2761 ggggtccctc tcgaggacgc catcttcgca ccccccaact gcacgaagac catagaagcg
     2821 gacatcccgc ccacgggtgt caccgtcctc atggggcatg tctttgacgg tgtcgcgcct
     2881 cacatggaac agtacccgcc ggaacgctcc ttcgttgttg ccgatgagga gcccgtcgat
     2941 gttgtgg
//
LOCUS       DAOPOA010000235         2940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_36694_length_2940_cov_2.819411, whole genome shotgun sequence.
ACCESSION   DAOPOA010000235 DAOPOA010000000
VERSION     DAOPOA010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36694_length_2940_cov_2.819411"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /gene="ychF"
                     /locus_tag="PKJ17_05825"
     CDS             <1..966
                     /gene="ychF"
                     /locus_tag="PKJ17_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOD75525.1"
                     /translation="GVVPVPDARLFSLARLLKPEKVTQTAIEFVDIAGLIKDAHKGEG
                     LGNRFLSHIRNVDAVAHVIRAFNSGQVPHVYADIDPARDMDIVETEIVISDLEIVEDR
                     IRKIERLAKISKDKGEAEMELLLRVRRYLEAGRFVATDDFDENERQALRAFDLITTKP
                     LFYIANVDEEGLSASPQPAIEEAAAKRGRQVVYICGKLEQDLSALPEDERASFMELYD
                     MKEFSIEKIIHVGYRILGLITFYTVVGKEMRAWTIPRGTTCAKAAGKIHTDMEKGFIR
                     AEVINIDDLITCGSEHAAREKGLLRLEGRDYLVEDGDILHIRFNV"
     gene            complement(994..2310)
                     /locus_tag="PKJ17_05830"
     CDS             complement(994..2310)
                     /locus_tag="PKJ17_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75526.1"
                     /translation="MQIEHQCPQCGAPVVLEETDRLLTCDFCKTRLYMQPRDYFRYCF
                     TPLDPFLEQVIYVPYWRFRGVHFSCRTSGITNGILDKTFLAVESKHFAQTLGLRPQAL
                     KLRFVQPADNARFIPPNIPFEKSFVEAKHNVTYETVTVHETRFVRRGTEGDYAEIPVT
                     RKELKEERLYHEAFVADTMSLIYTPMFVRNGRFHDAVLNEALYEGPPVYESFPYEFFS
                     GGWAINFLPTLCPECGWNTLAERDSCVLLCNGCNSAWRMDGQGLGRVGFATALSTKAA
                     PGSVRQYLPFWRLRVEVEGLKLDCYADLVRLGNLPRVIQPGWEEPPFFFWTPAFRMTP
                     AVYRRMSKQFTIANFSGEFHDRLPDAPVQAVNLTPQDALDSLITIIADFAINKKKVLP
                     LLPDMRITLREALLVLFPVTETDHEYIQPDINCGLMKNVLEQGKNI"
     gene            complement(2316..>2940)
                     /locus_tag="PKJ17_05835"
     CDS             complement(2316..>2940)
                     /locus_tag="PKJ17_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOD75527.1"
                     /translation="ILSRFNDYLGSNLDTLLDLPGQYDTMSAGLTQMLEKDFSHFGIQ
                     LSGLYINSITPPQEVQKAIDDKSRLAMFDDLNKLVKMKAAMAMESAAQNPGEAGAGMG
                     MGLGFMMPAMYAETLKGAPRSDGGMKCPECQNPINEDARFCPSCGHHLLVISQCPKCH
                     KNLPARARFCPACGQSVTAREELLKCPHCGTDNLSGSVFCNHCGEKI"
BASE COUNT          600 a          828 c          904 g          608 t
ORIGIN      
        1 ggtgtcgtcc ccgttcccga cgcgagactt ttcagcctgg cccgtctctt gaagcccgag
       61 aaggtcactc agacagccat cgagtttgtc gatatcgcgg gcctcatcaa ggacgcccac
      121 aagggggagg gtctggggaa caggttcctt tcccatatca ggaacgtcga tgccgttgcc
      181 catgttatcc gggccttcaa ctccggacag gtgccccacg tgtacgccga tatcgatccc
      241 gcccgggaca tggatatcgt ggagacggag atcgtcatct ccgacctcga gatcgtcgag
      301 gaccgcatac ggaagataga acggctcgcg aagatctcga aggacaaggg cgaagcggag
      361 atggaacttc tcctcagggt gaggcgctac ctggaggccg gccgcttcgt tgccaccgac
      421 gactttgacg agaacgagag gcaggccctg cgtgccttcg atctcatcac cacaaaaccc
      481 ctcttctaca tcgccaacgt cgatgaggaa ggtctgtcgg cgtccccgca acccgccatc
      541 gaggaagctg ccgccaagag gggaaggcag gtcgtctaca tctgcggcaa gctggaacag
      601 gacttgagcg ccctgccgga ggacgagcgg gcctccttca tggaactcta tgacatgaag
      661 gaattctcca tcgaaaagat catccatgtc ggctatcgca tcctcggcct catcaccttt
      721 tacactgtcg tgggcaagga gatgcgcgcc tggacgatac cccgggggac tacctgcgcg
      781 aaggccgcgg gcaagatcca cacggacatg gagaagggat tcatacgcgc cgaggtcatc
      841 aacatcgacg atctcatcac ctgcggcagc gaacacgccg cccgcgaaaa aggcctcctc
      901 cgcctcgaag gccgcgacta ccttgtggag gatggggaca tactccacat acgcttcaac
      961 gtttgacctg ggttcaagcg gctgcaacgg tctttagatg tttttcccct gttccagcac
     1021 gttcttcatg agaccgcagt ttatgtctgg ttggatgtat tcgtggtcgg tctcggtcac
     1081 agggaagagt acgagcagtg cctcgcggag ggttatcctc atgtcgggaa gaagggggag
     1141 gaccttcttc ttgttgatgg cgaagtcggc tatgatcgtt atcaggctgt cgagggcgtc
     1201 ctgaggggtg aggttaacgg cctgcacggg cgcgtcgggc agcctgtcat ggaattcacc
     1261 ggagaagttg gcaatggtga actgctttga catgcgccga tagaccgccg gcgtcatcct
     1321 gaacgcgggt gtccagaaga aaaagggagg ttcttcccag cccggttgta tgaccctggg
     1381 aaggtttcca aggcgtacca ggtccgcgta acagtcgagt ttcaaaccct cgacctcgac
     1441 ccggagccgc cagaagggca ggtattgacg caccgacccc ggagccgctt tcgtcgacag
     1501 ggcggtcgcg aaaccgacgc gtccaagacc ttgaccatcc atcctccagg cggaattgca
     1561 cccgttgcac aagaggacgc agctgtccct ttcagccagt gtgttccagc cgcactcggg
     1621 gcagagggtg ggcaggaagt ttatcgccca gccgccggag aaaaactcgt acggaaatga
     1681 ctcgtacacg ggagggccct cgtagagcgc ttcgttgagt actgcatcat gaaaacggcc
     1741 gttgcggacg aacatcggcg tgtagataag gctcatcgtg tccgcgacga aggcttcgtg
     1801 gtagagcctt tcctctttca gctcctttct cgttacgggg atctccgcgt agtccccctc
     1861 tgtcccgcgc cggacgaaac gggtttcgtg aactgtcacc gtctcgtaag tcacgttgtg
     1921 ttttgcctcc acaaaagact tctcgaaagg gatattggga ggtatgaatc tggcgttgtc
     1981 cgcgggctgc acaaagcgca gtttcagcgc ttgcggccgt aatccaaggg tttgcgcgaa
     2041 gtgcttgctt tcgacggcaa ggaaggtctt gtccaggatc ccgttggtta tcccgcttgt
     2101 cctgcaggaa aaatgcacac ccctgaaacg ccagtagggc acgtagatca cctgttcgag
     2161 aaaagggtcc aggggggtaa agcagtatcg aaagtagtcc cggggctgca tgtagagcct
     2221 ggtcttgcag aaatcgcagg taagcagcct gtcggtctcc tcgaggacca ccggggcgcc
     2281 gcattggggg cactgatgtt cgatctgcat gatggctaga tcttttcccc gcagtggttg
     2341 cagaagaccg atcccgacag gttgtcagtc ccgcaatgag ggcacttcag aagctcttcc
     2401 cttgccgtca cactctggcc gcaggcagga cagaaccttg cccgtgcggg gaggttcttg
     2461 tggcacttcg ggcactggga gatcacgaga aggtggtgtc cgcaggacgg gcagaaccgg
     2521 gcatcctcgt tgatagggtt ctggcactcg gggcatttca taccaccgtc ggatcgcggt
     2581 gctcccttga gggtctccgc gtacatggcg ggcatcatga agccgagtcc catgcccatg
     2641 cccgcgcccg cttcgccggg gttctgagcg gcgctttcca tcgccatggc agccttcatc
     2701 ttgaccagct tgttaaggtc gtcgaacatg gccagcctgc ttttgtcgtc gatcgccttc
     2761 tggacctcct ggggaggggt gatggaattg atgtagaggc cggagagctg gatgccgaaa
     2821 tgggaaaagt ccttctccag catctgtgtc agtcccgccg acatggtgtc gtactgaccg
     2881 ggcaggtcga ggagggtgtc gaggttgctg ccgagatagt cgttgaagcg ggagagtatg
//
LOCUS       DAOPOA010000236         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_36870_length_2928_cov_3.453533, whole genome shotgun sequence.
ACCESSION   DAOPOA010000236 DAOPOA010000000
VERSION     DAOPOA010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36870_length_2928_cov_3.453533"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..551
                     /locus_tag="PKJ17_05840"
     CDS             267..551
                     /locus_tag="PKJ17_05840"
                     /inference="COORDINATES: protein motif:HMM:NF038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HOD75528.1"
                     /translation="MVCTTLKPGIECLFMKKTGCSYSGGKCFTIVENCEGCTRVVEFE
                     TGKYCSSFPYPAQKWQKGSCPMSTHVKKAPKAEEQKINPLKASKRAAGKK"
     gene            753..1019
                     /gene="rpsT"
                     /locus_tag="PKJ17_05845"
     CDS             753..1019
                     /gene="rpsT"
                     /locus_tag="PKJ17_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOD75529.1"
                     /translation="MRKNKSAIKRAMQSEKRRLRNSHFKSTMKSRIKRAIGAIDRKEG
                     EVEAIFRDAVSSIDKAASKGVVHRNNAARKISRLARKLHKSQVA"
     gene            complement(1027..1545)
                     /locus_tag="PKJ17_05850"
     CDS             complement(1027..1545)
                     /locus_tag="PKJ17_05850"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOD75530.1"
                     /translation="MNGKGLRRTGLVLVAAAMLMVTGACGYQLIGGKGIYGGDVSSIY
                     VPVFKNLTYEPHVSQPVTESFSRELLSTGLFTLSYEKADGILKGEITDVAMAPSSLNA
                     QGVVIEKSVSVTVKVSLSQKNGALIKEFAISDSEIYKIQEGAEEYNRREAIRRLSARM
                     ARRFSAQLLLEY"
     gene            complement(1611..>2928)
                     /locus_tag="PKJ17_05855"
     CDS             complement(1611..>2928)
                     /locus_tag="PKJ17_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOD75531.1"
                     /translation="RFDGMNNREAIGAIIDYLEEKGFGTRTVNYRLKDWGISRQRYWG
                     APIPIIYCDDCGTVPVPEKDLPVVLPVDLEIRMVGRSPLADHAPFYEVECPVCKKKAR
                     RETDTMDTFVESSWYFLRYACADYQDGPLDRKRVDYWMPVDQYIGGIEHAVLHLLYSR
                     FFNRVLNELGLVGVREPFKNLLTQGMVIKDGAKMSKSKGNVVDPNYLIEKYGADTARL
                     FCLFASPPERDLDWSDQGVEGSYRFLQRVWRLAAEHAGSLAAVTAVDQIPGAGGDEKQ
                     LIHKIHRTIMKVTDDVERFHLNTAIASIMELVNMTYKFVEKGMDGERGKALLKGSIEA
                     ILRLLYPFVPHIVSELWQTMGGDVVLLRSSWPSWDAELVKEEKVMIAVQVNGKLRDTC
                     EADRGMGEDALKEMVFGLEKVQRHLEGKAVRKTIVVPNKLINIVCG"
BASE COUNT          676 a          848 c          763 g          641 t
ORIGIN      
        1 ccgcaacctg ctgattacaa atcagctgct ctgccgattg agctacacca gcaaaagagt
       61 tattatacta caagaaacat gccaaaatca acatgaagtt ccgcgcgaac aaaacaataa
      121 taattaaaat attttgcccc tctgtgtcaa tagattcctt cgccaatcac atttcagtcc
      181 gaggggttca gggagcatat tgacaatgag caccaaaatg tagttacata gtacgctttt
      241 gtcaattcta aggaggtgat gccgttatgg tatgcacaac gttgaaaccg ggaatcgaat
      301 gtctcttcat gaagaagacg ggatgttcgt acagtggagg aaaatgcttc acaatcgtgg
      361 agaattgtga aggatgcacc agggtcgtcg agttcgaaac gggaaagtac tgttcgagtt
      421 tcccttatcc tgcccagaaa tggcagaaag gctcgtgccc gatgtcaacc cacgtgaaga
      481 aagcgcccaa ggctgaagag caaaagatca atcccctcaa ggcatccaag agggctgcag
      541 gcaagaagta ggaaccgcgg acgtttagga tcgctggacc acataagaca agccgcctcg
      601 atgaaaagga tcccgcaggc atcgccgggt cgtttttgcg gtgtcgtgtg catatgccga
      661 caaagggacg cgtttttaaa tgatgcagaa ctatttgact ttcgggtcca tggatgataa
      721 ataaagaaaa gtcttaaaga ggaggacaac ctttgaggaa gaataaatcc gccatcaagc
      781 gagcaatgca gtccgagaaa cgcaggctca ggaattccca cttcaaatcg acgatgaaaa
      841 gcaggataaa acgggccatc ggcgcaattg acaggaaaga aggagaggtc gaggccatat
      901 tccgggatgc cgtttcctcc atcgacaagg cggcttcaaa gggtgtcgta cacaggaaca
      961 acgctgcgag aaagatctcc cgtctggccc ggaagcttca caagagtcag gtggcctaag
     1021 gcttcatcaa tactccagga gcaattgagc gctaaaccgc ctcgccatcc gcgccgagag
     1081 cctgcggatg gcctcgcgcc tgttgtattc ctccgcccct tcctgtatct tgtagatctc
     1141 tgaatcactg atggcgaatt cttttatgag ggccccgttc ttctgggaaa gggagacctt
     1201 taccgtcaca cttacggatt tctcaatgac gacgccctga gcgttcaggg atgacggtgc
     1261 catcgcgacg tccgtgatct cgcccttcaa gataccgtcg gccttttcgt agctcaacgt
     1321 gaagagtccc gtcgacagaa gttccctgga aaaagactcc gtgacgggct gggacacgtg
     1381 gggctcgtag gtcaggttct tgaagaccgg cacgtagatc gacgacacgt cgccgccgta
     1441 tatgcccttc ccaccgatga gttgatagcc gcacgcccct gtcaccatca gcatcgctgc
     1501 cgcgacgagg accagtcctg tccgtctcaa gcctttaccg ttcatagtcc attccattac
     1561 cttcattcct ctctttgccc gtcgcccacg acccggcacc ctgccctttt ctatccgcac
     1621 acgatattga tcagcttatt gggcaccact atggtcttcc tgacggcctt gccctcgaga
     1681 tgcctctgca ctttttccag gccgaagacc atctccttga gcgcatcctc ccccatgccc
     1741 cggtcggctt cacaggtgtc gcggagcttt ccgttgacct gcacggcgat catgaccttt
     1801 tcctctttca ccaactccgc atcccaggac ggccacgacg acctgagaag gaccacatcc
     1861 cctcccatgg tctgccacag ctcggacacg atgtgaggga cgaagggata gaggagtctc
     1921 aggattgcct caatggagcc cttgagcagc gccttccctc tttcgccgtc cattcccttc
     1981 tcgacgaatt tgtacgtcat attgacaagc tccatgatgc tcgcgatggc ggtattcaga
     2041 tggaaccgct ccacgtcgtc cgtcaccttc atgatggtcc tgtggatctt gtgtatgagc
     2101 tgtttctcat caccgcccgc tcccgggatc tggtcgaccg ctgtcactgc cgccagggaa
     2161 cccgcgtgct cggcagccag cctccagacc ctctggagga agcgatagct cccttccacg
     2221 ccctggtcgc tccagtcgag gtcccgctcc ggcggggagg caaaaaggca gaaaagccgt
     2281 gccgtgtcgg caccgtactt ctcgatgaga tagttggggt cgacgacatt ccccttggat
     2341 ttgctcatct tggccccgtc cttgatgacc atgccctggg tgaggaggtt cttgaagggt
     2401 tcgcggacac cgaccagacc gagttcgttg aggacgcggt taaagaagcg tgagtagagc
     2461 aggtgcagga ccgcgtgctc gatgccgccg atgtactggt ccaccggcat ccaatagtcg
     2521 acgcgcttcc tgtcgagagg gccatcctga taatcggcgc aggcgtaccg caggaaatac
     2581 catgacgact ccacgaatgt gtccatcgtg tccgtctccc gccgtgcctt tttcttgcac
     2641 acgggacact ccacttcgta gaacggcgcg tggtcggcga gcggcgagcg gcccaccatc
     2701 ctaatctcga ggtcgacggg caacaccacc ggcaggtcct tctcgggcac cgggacggtc
     2761 ccgcagtcat cacaatagat gatgggtatg ggagcccccc agtagcgctg tctcgagatg
     2821 ccccagtcct tcaaccggta attgacagtc ctcgtcccga aacctttctc ttcgagatag
     2881 tcgatgatgg cgccgatggc ctcacggttg ttcataccgt caaacctg
//
LOCUS       DAOPOA010000237         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_36885_length_2927_cov_2.668872, whole genome shotgun sequence.
ACCESSION   DAOPOA010000237 DAOPOA010000000
VERSION     DAOPOA010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36885_length_2927_cov_2.668872"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /gene="ppsA"
                     /locus_tag="PKJ17_05860"
     CDS             <1..1359
                     /gene="ppsA"
                     /locus_tag="PKJ17_05860"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOD75532.1"
                     /translation="SSLKTYKLKKRSKRLVTGLSIGEAIAAGEACKIKSAENVGRFKE
                     GSVLVTEMTDPDWVPIMKRAKGIVTDYGGRTCHAAIVSRELGIPAIVGTGNGSEVLKD
                     GQEVTVSCAEGDQGYVYEGILEYEETEVDLKKIPKIRTRIMMNIASPAAAFRWWRLPC
                     EGIGLARMEFIINNIIKIHPMALVRYDKLEDKEARQRIGELTIGYEDKTEYFVDHLSL
                     GIAKIAASRYPHQVIVRMSDFKTNEYANLIGGKEFEPREENPMLGFRGASRYYSERYR
                     EGFALECKAVKRVREKMGLRNVTIMIPFCRTLEEADRVMRVLAKNGLKRGENDLQVYV
                     MCEIPSNVILADQFARRFDGFSIGSNDLTQLVLGVDRDSAELAPLFDERNDAVKKMIQ
                     DLIKTAHRESCKVGICGQAPSDYPDFAAFLVKAGIDSISLNPDSIVEVKKRIARVRGK
                     RG"
     gene            1408..>2927
                     /gene="polX"
                     /locus_tag="PKJ17_05865"
     CDS             1408..>2927
                     /gene="polX"
                     /locus_tag="PKJ17_05865"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOD75533.1"
                     /translation="MPIHNREIADVFNRVADLLDIEGENQFRIRAYRKAARIVVDLPR
                     NVGDMVREGEDLTRFSGIGKDLAGKIKEIVETGVLGQLKELEKKTPPDLSRLMKMEGL
                     GPKRVQTLHKELGINDLDGLKKAAEENRIRKLEGFGEKTEKLILEALERMKDEGEKER
                     IKLFAAEQIANDYVDYLKKGRGIKDIAVAGSYRRRMETVGDLDILVTCKKGSQVMERF
                     VEYEDVAKVLSKGTTRSSVVLRTGLHVDLRLVPQVSYGAALHYFTGSRDHNIAVRGLG
                     VKLGLKINEYGVFRGKDEERVAGRTEEEVYRQVGLPYIEPELRENRGEIEAARNNKVP
                     ILIRRDDIRGDLHVHTNETDGRQTLEEMVQAAREQGYEYLAITDHSKRVTMAKGLDAR
                     RLAKQIDAIDTLNGKVRGIRILKGIELDILEDGSLDLPDDILKELDLTVCSIHYNQNL
                     SRKRMTERVIRAMDNPFFTIFAHPTGRLIGGRKGYEIDLEAIMKAAGERGGVLELNA"
BASE COUNT          768 a          701 c          934 g          524 t
ORIGIN      
        1 agctcattaa agacatacaa actcaagaag aggagcaaaa gactggtcac gggcctgagc
       61 ataggcgagg caattgccgc aggagaagca tgcaagatca aaagcgccga gaatgtcggg
      121 cgattcaaag agggttctgt cctggtcacc gagatgactg acccggactg ggtgcccatc
      181 atgaaacggg cgaagggaat cgtaacagac tatggaggtc ggacctgtca cgccgcgata
      241 gtgagccggg aactcggtat tccggcgatc gtgggaacag gcaacgggtc ggaggtctta
      301 aaggacggtc aggaggtaac ggtatcgtgc gcggaggggg atcaagggta cgtctatgag
      361 ggcatcctgg agtatgagga gacagaggtc gatctcaaga agatccccaa aatacgtacg
      421 cggatcatga tgaatattgc cagtccggcg gccgccttcc gatggtggag acttccctgt
      481 gaggggatcg gactagcaag aatggaattc atcatcaaca atatcatcaa gatccacccc
      541 atggcgctgg ttcgttacga taaattggag gacaaagaag cacgtcagcg catcggggag
      601 ctgaccatcg gatacgaaga caagacagag tactttgtcg accatctctc gctgggcatc
      661 gcgaagatag cggcatcccg gtatccccac caggtgatcg tccgaatgag tgatttcaag
      721 acgaacgaat acgccaacct tatcggggga aaggagttcg agcccaggga agagaacccg
      781 atgctcggat ttcgcggcgc ctcccggtat tacagtgaac gctatcgcga gggctttgct
      841 ctcgaatgca aggctgtcaa aagggtcagg gagaagatgg gattgaggaa cgttacgatc
      901 atgatcccat tctgcaggac gctggaggag gcggaccgcg tcatgcgggt cctggcaaag
      961 aatggtctga aacgaggcga gaacgatctt caggtctacg tcatgtgcga gattccctcg
     1021 aatgtcatac tggcagatca gttcgccaga cggtttgacg gattctccat cggaagcaac
     1081 gacctcacgc agttggtatt gggagttgat cgcgactcgg ctgaactcgc ccccctcttc
     1141 gatgaaagga acgacgcggt caaaaaaatg atccaggacc tcatcaaaac agcccatcgg
     1201 gaaagctgca aggtcggcat ttgcggccag gcgcccagtg attatcctga cttcgcagct
     1261 tttcttgtaa aggctggtat cgattctatc tccttgaatc cggacagcat cgtcgaggtg
     1321 aagaaacgga tcgcccgggt ccgggggaag aggggttgaa cgaacctcgt tgcgctggcg
     1381 atccactcaa aggctcagga gagcaagatg cccatacata acagggagat agcggacgtt
     1441 ttcaaccgcg tggccgatct actggatatc gaaggagaaa accagtttcg cattcgggcc
     1501 tatcgaaaag ccgcccgtat cgttgtggac ctgccgagaa atgtcggaga catggtaagg
     1561 gagggcgagg acctgacacg attctcgggc attggcaagg acctcgcggg aaagatcaaa
     1621 gagatcgtcg agacgggtgt acttggccag ctgaaggagc tcgaaaagaa gacgcccccc
     1681 gatctgagcc ggttgatgaa gatggaaggg ttgggtccga aaagggttca gacgctccac
     1741 aaagaactgg ggatcaatga tctcgacggc ctgaaaaagg cggcggagga gaacaggatc
     1801 cggaaactgg aagggtttgg agagaagacc gaaaagctga tcctggaggc cttggagcgg
     1861 atgaaggacg aaggggagaa agagaggatc aagctctttg ccgccgagca gatcgcgaat
     1921 gattatgtgg actatctgaa gaaggggaga gggataaagg acattgccgt ggcggggagc
     1981 taccggcgca ggatggagac tgtgggggac ctcgacatcc tggtaacgtg caagaaaggg
     2041 tcccaggtaa tggagcgttt cgtggagtac gaggatgtcg cgaaggtttt atccaaagga
     2101 accacccggt catcggtagt gcttcggaca gggctgcacg tcgacctgcg actcgtgccc
     2161 caggtgagct atggtgcggc gcttcactac ttcaccggct ccagggacca caacatcgcg
     2221 gtgcgcggcc tcggcgtgaa actcgggttg aagatcaacg aatacggcgt cttcagggga
     2281 aaagacgagg agcgggttgc cgggagaacg gaggaggaag tttacagaca ggttggtctt
     2341 ccctacatcg aaccggagct cagggagaac cggggggaga tagaggcggc ccggaacaac
     2401 aaagttccca ttctcatcag gcgtgacgat attcggggag acctccatgt gcacacgaat
     2461 gaaacagacg gccgtcagac cctcgaggaa atggtgcagg ccgcgcgtga acaagggtat
     2521 gaatatctgg ccatcaccga ccattcaaag cgggtcacca tggcgaaggg actggatgcc
     2581 aggcggctgg caaagcagat cgacgcaatc gacacgttga atggcaaggt cagggggatc
     2641 aggatcctca agggcattga gctcgatatc ctggaggacg gctccctcga ccttcccgac
     2701 gacatcctca aggaactcga tctgacggtc tgttcgatcc actacaatca gaacctttcc
     2761 cgaaaacgga tgaccgagcg ggtcatcagg gccatggaca atcccttctt cacgatcttc
     2821 gcgcatccca caggccgtct catcggcggg cggaaaggct acgagatcga tctggaggca
     2881 atcatgaagg cagcagggga gcgagggggg gtgttggagc tgaacgc
//
LOCUS       DAOPOA010000238         2903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37300_length_2903_cov_3.404494, whole genome shotgun sequence.
ACCESSION   DAOPOA010000238 DAOPOA010000000
VERSION     DAOPOA010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37300_length_2903_cov_3.404494"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKJ17_05870"
     CDS             complement(<1..204)
                     /locus_tag="PKJ17_05870"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOD75534.1"
                     /translation="MEKTRILFVCVHNSARSQMAEAFLNRLAGDRFHAESAGMDPGVL
                     NPIAVKVMKEEGIDISGNKTKSVF"
     gene            complement(331..1026)
                     /locus_tag="PKJ17_05875"
     CDS             complement(331..1026)
                     /locus_tag="PKJ17_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOD75535.1"
                     /translation="MEKIFIVLTGAVEGTPAIALGASFLWGVLSLVLSPCHLSSIPLI
                     VGFIDEQGRTSPKRAFCTASVFSSGIMISIGLIGLVTAMAGRMMGDVGRYGNYFVAFI
                     FFLVGLHLVGVIPMPWSGPGNINMKRRGLLAAFILGVVFGVALGPCTFAYMAPMLSIT
                     FQLSATKIWYGVLLLLAYAVGHCSIIVLAGTFTEAVQNYLNWNERSMGARIVKIVCGV
                     LVICGGIYLVYTA"
     gene            complement(1026..1487)
                     /locus_tag="PKJ17_05880"
     CDS             complement(1026..1487)
                     /locus_tag="PKJ17_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOD75536.1"
                     /translation="MKNRSMIFCMIILTMLSSAIMASTAVAAGEAMAGRKASASSKGP
                     AVPPAAVRYPALVDVGAKQCIPCKMMEPVLEELKAEYAGVLRVEFIDVQVDPQTATKL
                     GVRGIPTQIFYDASGKERYRHMGYMSKEGILAAFKKLGIELVRKDGGKKGK"
     gene            complement(1520..2254)
                     /locus_tag="PKJ17_05885"
     CDS             complement(1520..2254)
                     /locus_tag="PKJ17_05885"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOD75537.1"
                     /translation="MGGIYGARTLLCVKLDHVAELRQARSSDGFDPAVYAATCDRKGC
                     KGVSAHLGRDRRYMSVEDVHGIKDAIRGEFNLEIGLSDEMIGLAREVRPHIVTIVPEM
                     KEETTPGGGFDVKTHLSGIRDAVRLLHDDGIQVSFLIDPDIEALEYSKECAADRVEIC
                     MAGYCDARRKDEIDKEIRKVCAAVNHAAGMRLRVTAGRKLDYTNIEPLLDAAGLTEVH
                     IGRAIISRSVEVGLSRALDDMLEILE"
     gene            complement(2367..>2903)
                     /locus_tag="PKJ17_05890"
     CDS             complement(2367..>2903)
                     /locus_tag="PKJ17_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75538.1"
                     /translation="IAESVFIYLGIPFIAGVVTRFILIKVKDKTWYHERFIPKISPIT
                     LIALLFTILVMFSLKGEYIVKIPMDVVRIAIPLLIYFVFMFVVSFYMSAKARAHYGQA
                     ATLSFTAASNNFELAIAVAVAVFGINSGQAFAAVIGPLVEVPVLINLVHLSLWIKKRY
                     FPYAVETPTGVCYMSCKE"
BASE COUNT          639 a          894 c          756 g          614 t
ORIGIN      
        1 aaacacactt ttcgtcttgt tgccggatat atcgatcccc tcttccttca tcacctttac
       61 ggcgatcggg ttcaacacgc cgggatccat ccccgcgctt tccgcgtgaa accggtctcc
      121 cgccagcctg ttgagaaacg cctcggccat ctggcttctc gcactgttat ggacacatac
      181 aaaaaggatc ctcgtcttct ccattgctcc acctccattt tgaccgatct cccctggaac
      241 gcgtaacgaa gggctgtcat gcgttcacac taagccttcc tgcccctctt ttcaaccccg
      301 gaaatgcccg gagcgtttcc ggcgtctgtc ttacgcggtg tagaccaggt agatcccgcc
      361 acatatgacg aggaccccgc agactatctt cacgatcctc gcccccatgg accgctcatt
      421 ccagttgagg tagttctgga cagcctcggt gaaggtgccg gcaaggacga tgatcgagca
      481 atgacccacc gcgtaggcaa ggaggagaag gactccgtac catatctttg ttgccgagag
      541 ttgaaaggtt atactcagca tgggcgccat gtaagcgaaa gtgcaggggc ccagcgccac
      601 accgaacacg acccccagga tgaaggcggc aagaaggcct ctcctcttca tatttatatt
      661 gcccggcccg gaccagggca tggggatgac gccgacaaga tggagcccga cgaggaagaa
      721 gatgaaagca acgaaataat tgccgtagcg gcccacgtcg cccatcatcc ttcccgccat
      781 cgccgtgaca aggccgatga gaccgatgct tatcatgatc cccgacgaga acacagaggc
      841 ggtgcagaaa gccctcttcg gcgatgttcg gccctgctcg tcgatgaagc ccacgatcag
      901 gggaatgctc gaaagatggc aggggctgag cacaagactt aggactcccc agaggaagga
      961 tgccccgagc gctatggccg gggtcccctc taccgcgccc gtcagaacta tgaagatctt
     1021 ctccatcact tgcctttctt gccgccgtcc ttcctcacca actcgatccc gagcttcttg
     1081 aaggccgcga gtatgccctc ctttgacatg taccccatgt gccggtaacg ttccttcccg
     1141 gacgcatcgt agaagatctg cgtaggtatt cccctcactc ccagtttcgt ggcggtctga
     1201 gggtccacct ggacatcgat gaactcgacg cggagaacgc ccgcgtattc agccttcaat
     1261 tcctccagga ccggctccat catcttgcag gggatgcact gtttcgcgcc gacatcgacg
     1321 agcgcggggt accgcacagc ggcgggagga accgcgggac ccttcgacga cgcggaggcc
     1381 tttcgacctg ccatggcctc acccgcggca acagcggtcg atgccatgat ggccgacgac
     1441 agcatggtga ggatgatcat acagaatatc atggatctat tcttcattcc gaactccttc
     1501 ctgttggtca aggtaatggt tactccagaa tttccagcat atcgtcgagt gcccgtgaaa
     1561 gacccacttc aacggacctc gatatgatgg cacggccgat atgcacctcc gtcagcccgg
     1621 cagcatcgag aaggggctcg atgttcgtat aatcgagctt ccggccggcc gtgacgcgca
     1681 acctcatccc tgcggcatga ttgacggcgg cgcagacctt ccttatctct ttgtcaatct
     1741 cgtccttccg ccgggcatca cagtatcccg ccatacatat ctccacgcgg tccgcggcac
     1801 attccttcga gtactccagc gcttcgatgt ccgggtcgat gaggaaggac acctgaatcc
     1861 cgtcgtcgtg gagaagcctg acggcatccc gtatgccgga cagatgtgtc ttcacgtcga
     1921 acccgcctcc cggggtcgtt tcttccttca tctccggcac gatcgtgaca atatgaggtc
     1981 tcacttccct tgcaaggccg atcatctcat ccgacaggcc gatctcaagg ttaaactctc
     2041 cccggatggc gtcctttatc ccatggacgt cttccacact catgtagcgc ctgtccctgc
     2101 caaggtgggc ggagacaccc ttgcaaccct tcctgtcgca ggttgccgca taaacggcag
     2161 ggtcaaatcc atctgacgag cgcgcctgac gcaattcggc aacatggtcc agtttcacgc
     2221 acagcagggt gcgcgctcca tagattcctc ccattggcat caaactcctc cttccccgtt
     2281 ctctcaacca acggtacggg gggtagtcct gaaccggtat gtccggccgg tccaacgacc
     2341 ggcaaaacga tatgtgtggt ttccgttcat tccttgcatg acatataaca aacacccgtg
     2401 ggcgtttcca ccgcataggg gaagtatctc ttcttgatcc acagcgacag gtgaacgaga
     2461 ttgatcagca ccggcacctc gacaagaggt ccgatgacgg cggcgaaggc ctgtcccgag
     2521 ttgatgccga aaacggcaac ggcaaccgcg atcgcaagct cgaagttgtt cgacgcggca
     2581 gtgaaagaca gcgtggctgc ctggccgtaa tgcgctctcg ccttcgcgct catgtagaaa
     2641 gaaacaacga acatgaacac aaagtagata agaaggggta tggcgatgcg caccacgtcc
     2701 atggggatct tcacgatgta ttcacccttc agggagaaca tgacaagtat ggtgaaaagc
     2761 aaggcgatga gcgttatggg gcttatcttc ggtatgaacc tctcgtggta ccatgtcttg
     2821 tccttgacct tgataaggat aaagcgcgtg acaacccccg cgatgaaggg tatgcccagg
     2881 tagatgaaaa cgctttccgc tat
//
LOCUS       DAOPOA010000239         2897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37396_length_2897_cov_3.914145, whole genome shotgun sequence.
ACCESSION   DAOPOA010000239 DAOPOA010000000
VERSION     DAOPOA010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37396_length_2897_cov_3.914145"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKJ17_05895"
     CDS             <1..212
                     /locus_tag="PKJ17_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75539.1"
                     /translation="MGAFYIFPRSPIPDDIAFVRELQKHHILAVPGVGFGTPGHFRLA
                     YCIPMDVIERSRKHFLELGLKNGRK"
     gene            complement(517..1227)
                     /gene="bioD"
                     /locus_tag="PKJ17_05900"
     CDS             complement(517..1227)
                     /gene="bioD"
                     /locus_tag="PKJ17_05900"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOD75540.1"
                     /translation="MMKSVFVTGTDTGVGKTIISSSIAAYLSTVKGLDVGVMKPFESG
                     IETAGDRGLDDTWILKTASGSEDDVNEINPYLFKAPLAPEPAAAEENASIDIDLVTSL
                     YDRLAARHDVVIVEGAGGLLVPITERFFYADLIRAWRAPVIVVSRLTLGTINHTLLTC
                     GHLRSIGAKVLGVILNDTEGAGDIASRTNPAMLKKYLDVPLLGVFPHTPDLFGKGVNR
                     ERLADLLARNIDCSVLED"
     gene            complement(1224..1433)
                     /locus_tag="PKJ17_05905"
     CDS             complement(1224..1433)
                     /locus_tag="PKJ17_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75541.1"
                     /translation="MWKVLANAVEMLIYAAVYIILALVAVKVIGATFTTDFEKKISEE
                     NNFALALICASLFTGLAILLSAIVR"
     gene            complement(1446..2087)
                     /locus_tag="PKJ17_05910"
     CDS             complement(1446..2087)
                     /locus_tag="PKJ17_05910"
                     /inference="COORDINATES: protein motif:HMM:NF003989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOD75542.1"
                     /translation="MAKFSKIPEATIRRLSSYLKCMVDLEGKSEKVASSAVIASICNV
                     NAAQVRKDFAYFGEFGIRGMGYNVKDLKRHIKEILGINREWRIAVVGVGNMGSALLVY
                     KDFLKQNYKIVAAFDLYPEQVIGRISERVGKPVEILHLNRLREVVQARNIEIGIITTP
                     PQEAQKVADLLIDANIKGILNFSPAPVRTSGEVKLRNLFFTSALDNLVYYLSN"
     gene            complement(2359..>2897)
                     /gene="dapB"
                     /locus_tag="PKJ17_05915"
     CDS             complement(2359..>2897)
                     /gene="dapB"
                     /locus_tag="PKJ17_05915"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOD75543.1"
                     /translation="MAAEKGVAHVIGTTGLKEDHMRTIREAKGARIVISPNMSIGMNV
                     LFDLAQRVSSVLGDAYDAEIVELHHRWKKDAPSGSALRIKDAVEAGQGTRAWIEVFGR
                     EGITGERKKDEIGVMSIRGGDIVGEHTLYFAGIGERLELTHKAWSRDNFARGALIAAK
                     WLIKQPPGIYSMKDVLGL"
BASE COUNT          596 a          820 c          822 g          659 t
ORIGIN      
        1 ccatgggtgc cttctacatc tttcccaggt cgcccatccc cgacgacatc gcctttgtga
       61 gggaactaca gaagcaccac atcctcgctg tccccggcgt cggcttcggc accccgggcc
      121 acttccgcct cgcctattgt atccccatgg atgtgataga gcgatcgagg aaacacttcc
      181 tcgaactggg actcaagaac gggaggaaat aagaaacatc tcacgcccgc ttcgctcgag
      241 tgcacggagt ttctgaggag agcacacaga gaaaagattc ggccagattc cggcgcccgc
      301 cggagtctgg ccgtctttgc atcccgtctc cgacgggagg gaaagcagag gatgcttttc
      361 aggtggaggg atgggagtgc gggggggtga gggagtatgg ggtttgagga gactatagga
      421 cctatgacta caggggggga tgagaggtgg atactttttc aggataatag ggctatgagg
      481 agcgaacgcg acgaacgggc gtgagataag atcttctcag tcttctaata cgctgcagtc
      541 gatgtttctt gccaggaggt ccgcgagtct ttccctgttt acgcccttcc cgaagaggtc
      601 aggcgtgtgg gggaaaaccc ccaggagggg gacgtcgagg tatttcttca gcatggcggg
      661 gttcgtccgc gaggctatat cgcccgcacc ctccgtgtcg ttgaggatca cgccgagcac
      721 cttcgcaccg atggacctca ggtggccgca tgtcaggagg gtgtggttga tggtccccag
      781 ggtaagtctc gacacgacga tgacgggggc cctccatgcc cggatgaggt cggcatagaa
      841 gaagcgttcc gtgataggga cgagaagccc acccgcgccc tcgacgatga ccacgtcatg
      901 gcgggccgcg aggcgatcgt acaatgatgt gacgagatcg atgtcgatgg aagcgttctc
      961 ctcggcggca gccggttcag gcgcgagcgg cgccttgaag aggtaggggt ttatctcgtt
     1021 gacatcgtct tccgagcctg acgccgtttt gaggatccac gtgtcgtcga ggcccctgtc
     1081 gcccgctgtt tcgatgccgc tttcgaaggg cttcatgacc cccacatcga ggcctttcac
     1141 tgtcgagagg taggcggcaa tcgacgagga gatgatggtc ttccccacac cggtatcggt
     1201 acccgttaca aaaacacttt tcatcatctc actatggcgg agagaaggat cgcaagaccc
     1261 gtaaagaggg acgcgcatat gagggccagc gcgaagttgt tctcctcgga gatcttcttt
     1321 tcaaaatcgg tggtgaaggt agcgccgatc actttcacgg caacgagggc gagtatgata
     1381 tacaccgccg catagatgag catctccacc gcatttgcga gaaccttcca catggtcgcc
     1441 tctggctagt tcgacaaata atataccaga ttgtccagcg cggatgtgaa gaaaagattt
     1501 ctcagtttca cctcccccga ggtccgcacg ggtgccggcg agaagttgag aatgcccttg
     1561 atgttcgcat cgatgagcag atcggcgacc ttctgggcct cctgcggagg cgtcgttatg
     1621 atgccgatct ctatgttgcg ggcctgcacg acctctctga ggcgattgag atgcaggatc
     1681 tccaccggtt tgcccacgcg ctcggagata cgcccgatca cctgttccgg ataaagatca
     1741 aaggcggcaa cgatcttgta gttctgtttg aggaaatcct tatagaccag cagggcgctt
     1801 cccatgttac cgacaccgac gacggctatg cgccattccc ggttgatccc cagtatctct
     1861 ttgatgtggc gcttcaggtc cttcacgttg tagcccatgc cgcggatgcc gaattctccg
     1921 aagtaggcga aatcttttcg cacctgcgcc gcgttgacat tgcagatgct ggcgatcacg
     1981 gcgctcgagg cgaccttttc gctcttgccc tcgagatcga ccatacactt gagatagctc
     2041 gaaagccttc gtatggtcgc ttcgggaatc tttgagaatt ttgccattgt cccttacctt
     2101 ctctgtctga attttccctt ttgtttccta tatgtaacag ttccaagggg gttttgcaag
     2161 agaaaaattt aacaagcaaa ctcccccgcg cgcacccgcg ttatacctga tttcacaggg
     2221 gataaggtgt tggagattaa atgttaagtc ttaagttgaa agacctcgga tatccgcacg
     2281 ccgcccccct catacgtttc gtgctataca atgtctccaa tagcttgaaa ctcaaaacct
     2341 aaaacataaa actatttttc acagccccag gacgtccttc atggagtaga tccccggggg
     2401 ttgtttgatg agccacttgg ccgcgatgag cgctcccctg gcgaagttat cccgcgacca
     2461 ggccttgtgg gtcagctcga gccgctcgcc gataccggca aaataaaggg tatgctcccc
     2521 gacgatatcc ccaccgcgga tgctcatgac accgatctcg tccttcttgc gctcgcctgt
     2581 gatcccctcg cgcccgaaga cctcgatcca ggcgcgggtt ccctgtcctg cttcgacggc
     2641 gtccttgatc ctcagggccg aaccgctggg ggcgtccttc ttccaccgat ggtggagttc
     2701 gacgatctcg gcatcgtagg cgtctcccag gacggaggaa accctttggg ccaggtcgaa
     2761 gaggacgttc atgccgatgc tcatattcgg agagataacg atccgggcgc cttttgcctc
     2821 cctgatcgtc ctcatgtggt cctctttcag gcccgtcgtg ccgatgacgt gggccacgcc
     2881 cttttcggcc gccatcc
//
LOCUS       DAOPOA010000240         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37415_length_2896_cov_4.052446, whole genome shotgun sequence.
ACCESSION   DAOPOA010000240 DAOPOA010000000
VERSION     DAOPOA010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37415_length_2896_cov_4.052446"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..714
                     /gene="rplD"
                     /locus_tag="PKJ17_05920"
     CDS             91..714
                     /gene="rplD"
                     /locus_tag="PKJ17_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOD75544.1"
                     /translation="MAVTDILNIEGAKVGEIELKDEIFNCEVKEHLIHDVVKMQLARR
                     RRGTAKTKGRSEVSGGGRKPYKQKGTGQARRGSSRSPVMVGGGTIFGPQPRDYGYTVP
                     RKVRRSALRSALTVRNTASNMKVLDKLELANISTKTFFGIVKTLSLTKPLFIIDKKDE
                     TVERSARNIPYVKVLRVDGLNVYDIIRHEQLIMTVDAVKKVEEVLAS"
     gene            711..998
                     /locus_tag="PKJ17_05925"
     CDS             711..998
                     /locus_tag="PKJ17_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOD75545.1"
                     /translation="MNEYDIILRPIITEKSTLVKDTGNQYVFEVARSANKIEIRKAVE
                     KLFKVKVVDVHVSNMEGKKKRLGRFQGKRSDWKKAIVKLSPKDKITIFEGA"
     gene            1001..1828
                     /gene="rplB"
                     /locus_tag="PKJ17_05930"
     CDS             1001..1828
                     /gene="rplB"
                     /locus_tag="PKJ17_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOD75546.1"
                     /translation="MGVKEYKPTSAGRRFMSGLTFDEISKDKPEKSLLKPIKKTGGRN
                     HSGKITTRHIGGGHKRRLRIIDFKRNKFEILGKVAGIEYDPNRSANIALIHYIDGEKR
                     YILAPLGVKAGDTVIASMKPDTEIKEGNALPLKYIPLGTFVHNIEMKPGKGGQMARSA
                     GSYAQIVAKEGEYGHLRLPSGGVRLVNLSCMATIGQVGNIDHENITIGKAGKPRWLGT
                     RPTVRGTAMNPVDHPLGGGEGRSKGGRHPCSPWGQLAKGQKTRKNKRTDKFIIKLRG"
     gene            1838..2128
                     /gene="rpsS"
                     /locus_tag="PKJ17_05935"
     CDS             1838..2128
                     /gene="rpsS"
                     /locus_tag="PKJ17_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOD75547.1"
                     /translation="MARSVKKGPFLDVKLAKKVDEAKETKGSKVIKTWSRRSTITPDF
                     IGLTFAVHNGKKFIPVFVTEEMVGHKLGEFSPTRTFHSHAGDRKSKVARKKD"
     gene            2133..2465
                     /gene="rplV"
                     /locus_tag="PKJ17_05940"
     CDS             2133..2465
                     /gene="rplV"
                     /locus_tag="PKJ17_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOD75548.1"
                     /translation="MEITAKTRMIRISPRKVRIVADLIRQKNVNEASGLLTYMPQKGA
                     FILKKLLDSAIANARQKKYVDVDNLYVKTIMVDGGPVLKRFMPRAMGRATKIRKRLSH
                     ITLILDEA"
     gene            2479..>2896
                     /gene="rpsC"
                     /locus_tag="PKJ17_05945"
     CDS             2479..>2896
                     /gene="rpsC"
                     /locus_tag="PKJ17_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOD75549.1"
                     /translation="MGQKTNPFGFRLGVIKTWDSKWFAARNYAKFLHEDLVIKKFLKK
                     KLYQAGISKIEIERAANKDKRAKINIHTSRPGLVIGRKGAEVENLKRELQRITDKEVI
                     LNITEVKRPETDAQLVAENVALQIERRVSFRRAMKKN"
BASE COUNT          789 a          748 c          881 g          478 t
ORIGIN      
        1 tcgatcccac acagcaaacg gttgatgccc ttatgaagct cgaactggcg gccggcgtcg
       61 atgtggagat caaatcttag gagtcaggtt atggcagtca cagacatact gaacatagag
      121 ggtgcaaagg ttggagagat cgagctgaag gacgagatct tcaactgcga ggtgaaagag
      181 catcttatcc atgacgtggt gaagatgcag cttgccaggc gcaggagagg gacagccaag
      241 acgaaaggtc gcagcgaggt ctccggcggt ggccgcaagc cctacaagca gaaggggaca
      301 gggcaggcac gccgcggttc ctcccgctct cccgtgatgg tgggcggcgg caccatattc
      361 gggccccagc cgagggatta cggctatacg gtgccgagaa aggtcaggag aagcgccctg
      421 cgctcggccc ttacggtgag gaacacggca tcgaacatga aggtcctcga caagctcgaa
      481 ctggccaata tcagcacgaa gacctttttc gggatcgtca agacactgag cctgacgaaa
      541 ccgctcttca tcatcgacaa gaaggatgag acggtggaaa gatcggccag gaacatcccc
      601 tacgtcaagg tgctcagggt ggacggactc aacgtctacg acataatccg ccatgaacag
      661 ctcatcatga ccgttgacgc ggtgaagaaa gttgaagagg tgctggcatc atgaacgaat
      721 acgatataat cctgcggccg atcattaccg agaaaagcac gctggtgaag gatacgggaa
      781 accagtacgt ctttgaggtc gcgagaagcg caaacaagat agagataaga aaggccgtcg
      841 agaagctctt caaagtcaag gttgtcgatg tccatgtgtc gaacatggag ggaaagaaga
      901 agaggctcgg ccggttccag ggcaagcggt cggactggaa gaaggccatt gtgaagctca
      961 gtccgaagga caaaatcacg atttttgagg gtgcgtaaac atgggcgtga aagagtacaa
     1021 acctacatcc gcgggcaggc gtttcatgag cgggctcacc ttcgatgaga tatcgaagga
     1081 caagccggag aaatccctgc tgaaaccgat aaagaaaacg ggcggacgga accacagcgg
     1141 gaagatcacc acgaggcaca tcggcggggg acacaagcgg cgcctgcgca taattgattt
     1201 caagcgcaac aagttcgaga tcctggggaa ggtggccggt atcgaatacg accccaaccg
     1261 ctcggcgaac atcgcgctca tccattatat cgacggcgag aagcgctaca ttctggcccc
     1321 gcttggcgtg aaggccggag acacggtgat cgccagcatg aagcctgata cggagatcaa
     1381 ggaaggcaac gcccttcccc tgaaatacat ccccctgggc accttcgtgc acaacatcga
     1441 gatgaagccc ggaaagggcg ggcagatggc gcgaagcgcc gggagctacg cccagatcgt
     1501 ggcgaaggaa ggcgagtacg gacacctgag gctcccctcc ggtggcgtgc ggctcgtcaa
     1561 cctttcctgc atggcgacga tcggtcaggt cggcaacatc gaccatgaga acatcaccat
     1621 cggcaaggcg ggcaagcccc ggtggctcgg cacgaggccc actgtccgcg gcaccgccat
     1681 gaaccccgtc gaccaccctc tgggcggcgg cgaaggcagg tccaaaggcg gcaggcatcc
     1741 ctgttccccc tggggccagc tggccaaggg tcagaagacc aggaagaaca aacggacaga
     1801 caagtttatc attaaactga gaggttagga ggttcttgtg gcacgttccg tcaaaaaagg
     1861 gccattcttg gatgttaagt tagcgaagaa ggtcgatgag gcgaaggaga caaagggttc
     1921 gaaggtgatc aagacatggt cgcgccgttc gacgatcacc cccgacttca taggtctgac
     1981 cttcgcggtc cacaacggca agaagttcat accggttttc gtaaccgaag aaatggtcgg
     2041 ccacaagctc ggtgagttct caccgacgcg gaccttccac agccatgccg gcgacaggaa
     2101 gtcgaaagtg gccaggaaga aggattgata tcatggaaat cacggcgaaa actcgaatga
     2161 tacgtatatc accgcggaag gtgcggattg ttgctgacct gatcaggcag aagaacgtca
     2221 acgaggcctc ggggctcctg acgtacatgc cgcagaaagg ggccttcata ctgaagaagc
     2281 tcctcgacag cgccatagcc aacgcacgtc agaagaaata cgtcgacgtc gacaacctgt
     2341 acgtcaagac catcatggtg gacggcggtc cggtactgaa acgcttcatg ccgagggcga
     2401 tggggcgtgc gactaagatc cgcaagagat taagccatat aacgctcatc ctggatgaag
     2461 catagacttg gaggttaaat gggtcagaag acaaaccctt tcggtttcag gctcggggtc
     2521 atcaagacct gggattccaa atggttcgcg gcacggaatt acgcgaaatt tctccatgaa
     2581 gacctggtga tcaagaagtt tctcaagaag aaactctacc aggccggcat atcgaagatt
     2641 gagatagagc gcgccgccaa caaggacaaa cgggcgaaga taaacatcca cacatcgagg
     2701 ccgggtctcg tgatcggacg gaagggcgcc gaggtcgaaa acctgaagcg cgaactccag
     2761 cggatcacgg acaaggaggt catcctcaac atcaccgagg tgaagcggcc cgagacggat
     2821 gcccagcttg tcgccgagaa cgtggcgctc cagatcgagc gcagggtttc attcagacgg
     2881 gcgatgaaga agaacg
//
LOCUS       DAOPOA010000241         2892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37479_length_2892_cov_3.323229, whole genome shotgun sequence.
ACCESSION   DAOPOA010000241 DAOPOA010000000
VERSION     DAOPOA010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37479_length_2892_cov_3.323229"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="PKJ17_05950"
     CDS             <1..1497
                     /locus_tag="PKJ17_05950"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOD75550.1"
                     /translation="EIASRIDSPATIVGFVDRSRSGIHNAAAVIYKGKIRGVFRKELL
                     PNYGVFDEKRYFLPGSRPRVFRYGKVLFGVVICEDMWFREGPIHVMAQAGAGLIFNIN
                     ASPYHAGKIYVREDVVRSRTREDRVWVAYGNLVGGQDELVFDGQSFVMDRSGTVVAAA
                     GAFREDLLVVDIPEKELLRKARPVSAAGQVTTVAARPGGLRPSVKKPLTPRPVTRLDV
                     VEEVYEALKLGLADYVRKNGFRGTVIGLSGGIDSALVAALAADALGSDNIVCVFMPSR
                     FTSRESSEDAFDLAKRLGVRIIEVPIDPVFSAYKASLATLFHGLGEDVTEENLQARIR
                     GNILMALSNKFGWLVLTTGNKSEMSVGYATLYGDMAGGFAVIKDVPKTLVYKLCTWRN
                     GLGAVIPERIISKEPTAELKADQKDRDSLPPYDHLDQVLKSYVEEDTEPSAADFPNLP
                     AEEIARVLRMVDLSEYKRRQSPPGIKITPKAFGKDRRMPITNKYKGSQ"
     gene            1494..2009
                     /locus_tag="PKJ17_05955"
     CDS             1494..2009
                     /locus_tag="PKJ17_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD75551.1"
                     /translation="MKFDFMVKRLSPVLKRITKRLNGHHAYFDEEDLYQEALTHLWGA
                     FRKGSIDDKTDSYILQGCYYHLKNHLRKVRENAVFVSLSEPAGEDGISWEEMIASDES
                     SSFARLEDKLKFQSITDACSSDRDRQVLTLLMEDLSVREVGTRLGISHVMVLKIRNRI
                     RDNYFRRIGEK"
     gene            2170..2421
                     /locus_tag="PKJ17_05960"
     CDS             2170..2421
                     /locus_tag="PKJ17_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75552.1"
                     /translation="MKKALVLFFAVVIGLSFAFTGFAQEKKAAPATPAAPATPAAKVE
                     APAPVEKEKPAKKEKKVKKTKKTKKAKGAPAEEKPAETK"
     gene            complement(2487..>2892)
                     /locus_tag="PKJ17_05965"
     CDS             complement(2487..>2892)
                     /locus_tag="PKJ17_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75553.1"
                     /translation="KPEMPVERGSETVLIVEDDEDFRKVEAEMLTRLGYRVYAAADGM
                     EGIALFAEKSEEIDLVILDVVMPRMSGRDALEGMRRIRPDIPSLFVTGYSLDGIHVNY
                     ILDQDIEALQKPFSFDTLSRKIREVMRNRGRG"
BASE COUNT          645 a          837 c          844 g          566 t
ORIGIN      
        1 gaaattgcgt cgcgtatcga ctcgccggcg acgatcgtcg gttttgtgga ccgctcccgg
       61 tcgggcatcc ataacgcggc cgccgtcatc tacaagggaa agatacgggg cgtcttccgc
      121 aaggaacttc ttcccaacta cggcgtcttc gatgaaaaga gatactttct ccccggcagc
      181 cgtccgaggg tgttccgcta cggcaaggtc ctcttcggcg tcgtgatctg tgaggacatg
      241 tggttcagag aaggccccat acatgtcatg gcccaggcgg gcgccgggct catcttcaac
      301 ataaacgcct ctccctatca tgcggggaag atttacgtcc gcgaggacgt cgtcaggagc
      361 aggacccgtg aggaccgggt gtgggttgcc tacggcaacc ttgtcggcgg ccaggatgaa
      421 ctcgtgttcg atggacagag cttcgtgatg gaccgctcgg gcacggtcgt cgccgcggcg
      481 ggcgcgttcc gggaagacct tctcgttgtc gatattccgg aaaaggaact cctcaggaag
      541 gcccgtccgg tctctgcggc cggacaggta accaccgtgg ccgcgcgacc cggcgggctt
      601 cgcccctcgg tcaagaaacc gctcaccccg cgtcccgtca cgcgtctcga cgtcgtggag
      661 gaggtatacg aggccttaaa gctcggcctt gccgactacg tgaggaagaa cgggttcaga
      721 gggaccgtga tcgggctctc cggagggata gattcggctc tcgtggccgc cctcgcggcg
      781 gacgcgctcg gcagcgacaa tatagtgtgc gttttcatgc cttccaggtt cacatcccgg
      841 gagtcctcgg aagatgcctt cgaccttgcg aaaagactgg gcgtgaggat catcgaggtg
      901 cccatagatc ccgtgttctc cgcctataag gcgagcctcg cgacgctctt ccacggcctc
      961 ggggaggatg ttacggagga gaacctccag gcgcgtatcc gcggcaacat tctcatggcg
     1021 ctctcgaaca agttcggttg gcttgtgctc acgacaggca acaaatcgga gatgagcgtg
     1081 gggtacgcga ccctgtacgg cgatatggcc gggggtttcg ccgttatcaa ggatgtcccg
     1141 aagacgctcg tttacaagct ctgcacatgg cgcaacggtc ttggtgcggt cattcccgag
     1201 aggataatat cgaaagagcc gacggcggag ctgaaggccg atcagaagga ccgcgacagt
     1261 cttccgccct acgaccatct ggatcaggtg ctgaaaagct acgttgaaga ggacacggaa
     1321 ccttccgcgg ccgattttcc gaacttaccg gcggaggaga tcgcccgcgt tctgagaatg
     1381 gtggacttaa gcgaatacaa gcgccgccag tccccgccgg gcatcaagat cacacccaag
     1441 gcattcggca aggacaggag gatgcccatc accaacaagt ataagggttc gcaatgaaat
     1501 tcgattttat ggtaaaaagg ctctcaccgg tcttgaagag gataacgaag agattgaacg
     1561 gccatcacgc gtattttgac gaggaagacc tctaccagga ggcgctgacc cacctttggg
     1621 gcgccttccg caaggggtcc atcgacgaca agacggacag ctacatcctg cagggctgct
     1681 actatcacct caagaaccat ctccggaaag tgcgcgagaa cgcggttttt gtgagcctca
     1741 gcgagccggc gggggaggac ggcatttcct gggaggagat gatagcctcc gacgaatcct
     1801 cgtccttcgc gcgcctcgag gacaaactga agttccagtc gatcaccgac gcctgcagtt
     1861 cggaccggga ccgccaggtc ctcacgcttc tcatggaaga cctgtcggtg agagaggtgg
     1921 ggacacgcct cggcatctcc cacgtcatgg tcctcaagat ccgcaacagg atccgcgaca
     1981 actactttcg aagaataggg gaaaagtagc ctcaggcctc aagcagagac caaagacccc
     2041 gggaaagcac gctctcccat cacccatgac ctatgaccca tcacctgtat ttttttctat
     2101 cttttatttt cgacttggtt tatatatctc tctaatgttc ccaatacaag aggaaggagg
     2161 tgaaacagaa tgaagaaggc attggtactg ttctttgcag tcgtgatcgg tctttccttt
     2221 gcatttacag ggtttgcgca ggagaagaag gcagcccccg cgacgcccgc cgcccccgcg
     2281 acgcccgccg ccaaggtaga ggctccggcc ccggttgaga aagagaaacc ggcgaagaag
     2341 gaaaagaagg taaaaaagac gaagaagacg aagaaggcca agggagctcc cgccgaggag
     2401 aaaccggccg aaacgaagta ggcaagcaag caaatacccg gaaaactgtt tcacgaaagg
     2461 ggagaagaga ttctcccctt ttcatctcat cccctgcccc tgttccgcat cacctcccgt
     2521 atcttccggg agagggtatc gaaggagaaa ggcttctgaa gggcctcgat atcctgatcg
     2581 agaatgtagt tcacgtgtat gccgtcaagg ctgtaccccg tcacgaacag tgaggggatg
     2641 tcggggcgga tcctccgcat gccttccagg gcgtcccggc cgctcatccg cggcatcacg
     2701 acgtcaagga tgactagatc tatctcttcg cttttttcgg cgaagagcgc tattccctcc
     2761 ataccgtcgg ctgcggcata cacgcggtag ccgaggcgcg tgagcatttc cgcctcgacc
     2821 ttcctgaaat cctcatcgtc ctcaacgatg agaaccgtct cgctccctct ctcaacgggc
     2881 atctcgggtt tc
//
LOCUS       DAOPOA010000242         2884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37601_length_2884_cov_3.522093, whole genome shotgun sequence.
ACCESSION   DAOPOA010000242 DAOPOA010000000
VERSION     DAOPOA010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37601_length_2884_cov_3.522093"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..848
                     /locus_tag="PKJ17_05970"
     CDS             60..848
                     /locus_tag="PKJ17_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOD75554.1"
                     /translation="MNAPHMLYSRDGNVAVLTINRPEARNAFSPEMIRLWREHLDEAR
                     RDDGVTVIVVTGTGDTFCSGGDIRDMAEGKLRSWDMKRFLWEGVHRIILTLEDLDKPV
                     IAAINGAAMGAGMDMAIMCDLRVCSDRAKLGESYIQMGLIPGDGGAWFLPRLVGVSKA
                     LELLLTADVIDAGQALSLGIVNRVVPHENLMEETMKLARRIAGKPPLAVRMMKRAVYQ
                     GTTGTLRSHLDYISSQMSLLSETADHAEAARAFLEKRKPVFRGG"
     gene            1115..2008
                     /locus_tag="PKJ17_05975"
     CDS             1115..2008
                     /locus_tag="PKJ17_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOD75555.1"
                     /translation="MGKLSSTLALAAIIVSLVFPLFVSSQSTDVLYPETKQVITIVNE
                     ASQEIAQKGEAAFVEFRKKGSRWLHDDTYVFVIDREGKVILNPTRPELEGKNQLNLRD
                     AVGKPFIKWFIKEVTDYPGKAQGWSHYVWFKPGQEIPSWKTSFVRYVKAPSGKGYIVG
                     SGLYEMRPERAFVVDIVDEAAYIVGKEGRRAFPALRDKTGEFNYLTTYVFVIDANGVD
                     LVNPAFPQHEGRNVLALKDSKGKFFIRDMIAMLANKDSGWITYSWPKPRTTKPSDKET
                     YVKKVRSGNDVFYVGSGIYLE"
     gene            2321..>2884
                     /locus_tag="PKJ17_05980"
     CDS             2321..>2884
                     /locus_tag="PKJ17_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75556.1"
                     /translation="MNSQFLPIPEITTTMAVTSSIGIAVLLGISILLGLLGKKQWKSF
                     DEYLVGKRDMGPVVTGCALSASYLSGWAFCGSTGIVYSVGFSGMWFAGMWSLIGVIPC
                     LWLASAKTREFAVKLGAATVPELMGRRYESKALQTVVSISMLFFLFIYSVGQLKAAGG
                     VWYAVTGFPPALCLFLGVFIAWIYMVLG"
BASE COUNT          623 a          797 c          860 g          604 t
ORIGIN      
        1 ggtgtttact gtatggggtc cttcaaggga catatctaac cacgaaaagg atgaaaacga
       61 tgaatgcacc gcacatgctt tattcccggg acgggaacgt ggccgtactc accatcaacc
      121 ggccggaagc ccggaacgct ttcagtcccg agatgatacg gttgtggcgc gagcacctcg
      181 acgaggcgcg gcgtgacgac ggcgtcaccg tcatcgtcgt cacgggcacg ggggatacct
      241 tctgctcggg gggtgacatc cgcgacatgg ccgaagggaa gctcaggtcc tgggacatga
      301 aacgatttct ctgggaaggg gtgcaccgca tcatcctcac cctggaagac ctcgacaagc
      361 ccgttatcgc cgcgataaac ggcgccgcca tgggagcggg gatggacatg gccatcatgt
      421 gcgacctgag ggtctgttcc gacagggcaa agctcggcga atcgtacatt cagatgggtc
      481 tcatccccgg tgacggaggc gcatggttcc tgccgaggct tgtcggtgtt tcgaaggcgc
      541 tggagcttct tcttaccgcc gatgtgatcg acgccggtca ggcgctttcg ctcggcatcg
      601 tcaaccgcgt ggtcccccac gaaaacctca tggaagagac gatgaaactc gcccgcagga
      661 tcgccgggaa gccgccgctc gcggtgcgga tgatgaagcg ggcggtctac cagggcacaa
      721 ccggcacttt gagatcgcac ctcgactata tctcctcaca gatgtccctc ctttccgaga
      781 ccgcggacca cgccgaggcg gcgcgggcct ttctcgagaa gaggaaaccg gtgttcaggg
      841 gagggtgatc gttcgggttg cgggttgcgg gttgcgggtt gcgggaaaaa gacctggaga
      901 acaggtcgaa cgtcaacaca cgcttcggat tggaaataat gaagacacac aagcgttttt
      961 gttccggagg gttcgaggcg acagggatcg tttcgggaat ttttctgttt actcttcggc
     1021 tcatttatat tatgtatgtg tagggcttgg gctggtgtgt caataggggt tgtatctgcc
     1081 ttcgggcata ttgatatcac acagggggtt cactatgggt aagctcagct caacgctcgc
     1141 actcgcagca atcatcgttt ctctcgtgtt tcctctcttc gtttcctccc aatcaacgga
     1201 tgttttgtac cccgaaacga aacaggtcat caccatcgtt aacgaagctt cacaggagat
     1261 cgcgcagaag ggagaggcgg cctttgtgga gtttcggaag aaaggaagca ggtggctcca
     1321 tgacgatact tacgtgttcg tcatagacag ggagggaaag gtgatcctca accccacccg
     1381 tcccgagttg gaaggcaaga accagctcaa cctcagggac gccgtgggaa agcctttcat
     1441 caagtggttc atcaaggagg tgacggacta cccgggaaaa gcgcagggat ggtcccacta
     1501 cgtctggttc aagccgggcc aggagatccc ctcgtggaag acgtccttcg tcaggtacgt
     1561 caaggcgccg tcggggaaag gatatatcgt cggcagcggt ttgtatgaaa tgagacccga
     1621 aagggccttc gtcgtcgata tcgtcgacga ggcggcctac atcgtcggca aggaagggcg
     1681 ccgggcattc cccgcgctgc gcgacaaaac aggagagttc aactatctca ccacatatgt
     1741 cttcgtcatc gatgcaaatg gcgtcgacct cgtcaacccc gctttcccgc agcatgaagg
     1801 caggaatgtc ctcgccctga aggacagcaa aggaaagttc ttcatcaggg acatgatcgc
     1861 catgctcgcg aacaaggaca gcggctggat cacctattcg tggccaaaac cgaggaccac
     1921 aaaaccgtcc gacaaggaaa cctacgtgaa gaaggtcaga agcggcaatg acgtcttcta
     1981 cgtggggtcg ggcatctatc tcgagtaaaa gacttcgatc atcctgaaag caggcaaggg
     2041 cttcctccgc gggacgtgcc tcacggtgcc ggatccggta cgttgacgag agcgaaggac
     2101 cgccggaaga acgggatgac cggcattgtt cgtcaggctt tacgcgcaac gggtctgtac
     2161 ccttggcagc gggacaaaac acaaaagaaa aagggggtag cacaatgaat ccagttcaac
     2221 ctgatcctat gtttccctgg tacatctggt ggttgatgat catcggactc tttctcatga
     2281 ccttcatcgg cacgtggata gtcacaagga ggtgggacga atgaactcac aattcctgcc
     2341 cattcccgag ataacgacca cgatggcggt aacgtcgtcc atcggcatcg cggtccttct
     2401 cggcatctcc atccttctcg gcctcctcgg gaaaaaacag tggaagtcct tcgatgagta
     2461 cctcgtcggc aaacgggaca tgggtcccgt ggtcaccggc tgcgcgcttt cagcgtcata
     2521 tctctccggc tgggccttct gcggcagcac gggaatcgtc tattccgtcg gtttctccgg
     2581 tatgtggttt gccggcatgt ggagcctcat cggggtaatc ccctgcctct ggctagcctc
     2641 cgcgaagacg cgggagttcg ccgtgaagct aggcgcggcg acggtgccgg aactcatggg
     2701 gcgccgctat gagtccaagg ccctgcaaac ggtggtcagc atcagcatgc tcttcttcct
     2761 tttcatctat tccgtcggac agctcaaggc cgcgggcggc gtctggtacg ccgtgaccgg
     2821 ctttccccct gccctgtgcc tcttcctcgg cgttttcata gcctggatct acatggtgct
     2881 cggc
//
LOCUS       DAOPOA010000243         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37633_length_2882_cov_3.754510, whole genome shotgun sequence.
ACCESSION   DAOPOA010000243 DAOPOA010000000
VERSION     DAOPOA010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37633_length_2882_cov_3.754510"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /gene="lysA"
                     /locus_tag="PKJ17_05985"
     CDS             <1..900
                     /gene="lysA"
                     /locus_tag="PKJ17_05985"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOD75557.1"
                     /translation="MINIESEGELRLLAKLSRKMKKKVPVSIRVNPEIDPKTHPYITT
                     GLKKNKFGVLWGDALRLYREIAAEEYLVPVGISSHIGSQILELGPFVEAVTSLKSMIL
                     ELERQGIAVEMLDIGGGLGITYKDELPPDPAQYAAAIRENLGDTGVTLILEPGRVIVG
                     NSGIFVTKLLYVKGTAGKTFYIVDGAMNDLVRPSLYNAYHEILPVEEGKGKTQVVDLV
                     GPICESGDFFAKDRELASLKEGGLVAIKGAGAYGFAMSSNYNSRGRAAEVLVKGGEFF
                     VVRRRETYEDLTRGEAVPQFLEV"
     gene            906..1718
                     /gene="dapF"
                     /locus_tag="PKJ17_05990"
     CDS             906..1718
                     /gene="dapF"
                     /locus_tag="PKJ17_05990"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOD75558.1"
                     /translation="MTQLEFSKMSASGNDFVIVDNRDGKVYERFPDVVGFVAKICRFH
                     HSVGADGVILIEDSKDHDFRWRFFNADGSEAEMCGNGGRCAARFAFTNGIAGDRMSFE
                     TVAGVIKAEVHGLKVKLQLTRPADVKLDYAVALDDKELFLCSVNTGVPHAVILVDDVD
                     LVPVDELGRAIRYHKAFGAKGTNVDFVEVLDKENARLRTYERGVEGETFACGTGAVAS
                     GVILAEKTLVRSPVNIHTKGGEVLRIYIDGEVYLEGDARFVYNGRLHEEALL"
     gene            1730..2629
                     /gene="dapA"
                     /locus_tag="PKJ17_05995"
     CDS             1730..2629
                     /gene="dapA"
                     /locus_tag="PKJ17_05995"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOD75559.1"
                     /translation="MELKGIYTALVTPFRGGALDEKALKKLIQFQIAGGVDGIVPCGT
                     TGEAPTLSYEEHEKVIELAIKYAGGKVPVIAGTGSNSTAEAIELTASAKKLGADACLL
                     TTPYYNKPTQAGLLAHFTTIAEKTRVPLVLYNIPGRTGINMAPETIAELAKVRNIVGI
                     KEAAGSLTQVSEIYRLTKGRFAILSGDDNLFLPMMSVGAVGVISVISNIMPKELKSLY
                     KAFMVEKNIAKAIRIHTKLMPLFQGMFIETNPIPVKEAAYRMGMIAREYRLPLCPMSD
                     KNAAFLGGLLGEYGLAGKGRRKG"
     gene            2654..>2882
                     /locus_tag="PKJ17_06000"
     CDS             2654..>2882
                     /locus_tag="PKJ17_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75560.1"
                     /translation="MARVVITGACGKMGRAIVKLALEDPDLEIAGLVEVKGHPMTGQA
                     HDLMGGRAPFVSDEMPQVLTGADVVVDFTEAE"
BASE COUNT          627 a          810 c          920 g          525 t
ORIGIN      
        1 atgatcaata tcgaatccga gggcgaactt cgcctgcttg cgaagctttc ccggaagatg
       61 aagaagaagg ttcccgtctc gatccgcgtc aatcccgaga tcgatccgaa gacccatccc
      121 tacatcacga cgggcctcaa gaagaacaag ttcggcgtgc tctgggggga tgcgttgcgg
      181 ctttaccgcg agatagcggc cgaggaatat cttgtccctg tcggcatctc gtcgcacatc
      241 ggttcccaga tactcgagct cggccccttc gtcgaggccg tgacgtccct gaaatcgatg
      301 atcctggagc tcgaacggca ggggatcgcc gtcgagatgc tggatatcgg cggaggcctc
      361 ggcatcacgt acaaggatga gctcccgccg gaccctgccc agtatgccgc ggccatcagg
      421 gagaacctcg gtgacacggg tgtgacgctc atcctcgagc ccggaagggt catcgtcggc
      481 aacagcggga tcttcgtcac gaaactgctc tacgtgaaag ggaccgcggg aaagacgttc
      541 tacatcgtcg acggcgcgat gaacgatctc gtccggcctt ctctgtacaa cgcgtaccac
      601 gagatcctgc ctgtcgagga aggcaaggga aaaacgcagg tcgtcgacct cgtggggccc
      661 atctgcgaat ccggtgattt cttcgcgaag gaccgggagc tggcctccct gaaggaaggc
      721 ggtctcgtcg ccatcaaggg ggccggggca tacggtttcg ccatgtcgtc gaattacaac
      781 tcccggggca gggccgcgga ggtccttgtg aagggaggcg agttcttcgt ggtccgcagg
      841 cgcgagacat acgaagacct gacgcgcggt gaggctgtcc cgcaattcct ggaggtctga
      901 gaacggtgac gcagctcgag ttttcaaaga tgagtgccag cgggaacgat ttcgtgatcg
      961 tggacaaccg cgacggcaag gtctacgaac ggttccccga tgtcgtcggg ttcgttgcaa
     1021 agatatgcag gttccatcat tccgtgggtg ccgacggcgt gatactcatc gaggattcca
     1081 aagaccacga ttttcgctgg cggttcttca acgccgacgg ctccgaggcg gagatgtgcg
     1141 gcaacggggg tcgttgcgcc gcccggtttg ccttcacgaa cggcatagcg ggcgacagga
     1201 tgtcctttga gaccgtcgcc ggcgtcatca aggccgaggt ccacggcctc aaggtgaagc
     1261 tgcagctgac gcggcccgct gacgtgaaac tcgactacgc ggtcgctctt gacgacaagg
     1321 agcttttcct ctgcagcgtc aacacgggcg tgccccacgc ggtcatcctt gttgatgatg
     1381 tcgaccttgt gcccgtcgac gagcttggca gggcgatccg gtaccacaag gccttcggcg
     1441 cgaaaggaac gaacgtcgat ttcgttgagg ttctggataa agagaatgcc aggctcagga
     1501 cctatgagcg cggagtggag ggagagacct tcgcatgcgg aacgggggcg gtcgcgtcgg
     1561 gggtcatcct tgccgagaag acgctcgtca ggagccccgt caatatacac acgaaaggcg
     1621 gcgaggtgct caggatatac atcgacggcg aggtctatct cgaaggtgac gcgaggttcg
     1681 tctacaacgg ccggttgcac gaggaagcac ttttgtgaaa ggagaagcca tggaattaaa
     1741 aggtatttac actgcgcttg tcacgccttt tcgcggcggc gctctcgacg agaaggcatt
     1801 gaagaagctc atccagttcc agatagcagg gggtgtcgac ggcatcgtgc cctgcggaac
     1861 gacgggtgag gctcccacgc tttcctacga ggaacacgag aaggtcatag agcttgccat
     1921 aaagtatgcg ggaggaaaag tgcccgtcat cgcgggtacg ggttccaaca gcaccgccga
     1981 ggccatagaa ctcacggcgt cggcaaagaa gctcggggcg gacgcgtgcc tgctcaccac
     2041 gccgtactac aacaagccca cgcaggccgg gcttctggcc cacttcacga cgatagcgga
     2101 aaagacccgg gtacccctcg ttctttataa cataccgggc cggacgggca taaacatggc
     2161 cccggaaacg atagccgaac tggcgaaggt acggaacatc gttggcatca aggaggccgc
     2221 ggggtcgttg acgcaggtct cggagatcta tcgcctgaca aagggcagat tcgcgatcct
     2281 ctccggagac gacaaccttt tcctccccat gatgagcgtc ggggcggtgg gcgtcatatc
     2341 ggtcatctcc aatatcatgc cgaaagaact caagtccctg tacaaggcct tcatggtgga
     2401 gaagaacata gcgaaggcca tcaggataca cacgaagctg atgccccttt tccagggcat
     2461 gttcatagaa acgaacccca tacccgtcaa ggaggcggcc taccggatgg gaatgatcgc
     2521 gcgcgagtac cgcctgcccc tttgccccat gagcgacaag aacgccgcgt tcctcggtgg
     2581 tcttctcggc gagtacggcc tcgcgggtaa aggccgacgc aagggttagc cccggaaccg
     2641 ggaggtctgt ggtatggcaa gagttgtcat aacaggagca tgcggaaaaa tggggcgggc
     2701 catagtgaag ctcgccctcg aggacccgga ccttgagata gccggcctcg tcgaggtgaa
     2761 aggccatccc atgacgggac aggcgcacga cctcatgggc ggaagagccc cctttgtcag
     2821 cgacgagatg ccgcaggtgt tgaccggggc ggacgtggtc gtcgatttca cggaggcgga
     2881 gg
//
LOCUS       DAOPOA010000244         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37662_length_2880_cov_3.283186, whole genome shotgun sequence.
ACCESSION   DAOPOA010000244 DAOPOA010000000
VERSION     DAOPOA010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37662_length_2880_cov_3.283186"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1382)
                     /locus_tag="PKJ17_06005"
     CDS             complement(213..1382)
                     /locus_tag="PKJ17_06005"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOD75561.1"
                     /translation="MGGRKRIAVLTGGGDVPGLNVAIKAVVARADSRDIEVVGIRRGW
                     MGLLCIDPGSPETAQSLVMPLTLDKVRTIDRTGGTILHTSRTNPGNVNADEVPPFVEE
                     GDRVPTASGRIDCTRHILKVLESLAIDAIIPIGGDDTLSYAVRLHKEGVQVVAIPKTM
                     DNDVFGTDFCIGFSTAVTRSVDAINALRTTTGSHERICVVELFGRNSGETSLIAAYLA
                     DVDRAVISEVPFDLDKLVGFLVTDRSRNPSNYAIMTISEGAHTVGGTVTETGIEDAYG
                     HKKLGGVGYLVSQEIKQRSGVNVIYQQLAYMMRSGAPDSLDRMVAVSYGSLALDQIAM
                     GHRGRMVALQQAIYTTVPLDMVVAARKQVDVTALYDVDTYRPKVRDTLGKPMFLY"
     gene            1676..2719
                     /locus_tag="PKJ17_06010"
     CDS             1676..2719
                     /locus_tag="PKJ17_06010"
                     /inference="COORDINATES: protein motif:HMM:NF002327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOD75562.1"
                     /translation="MNIRERLEEREGKVLSPWAQKSAATRGRMREEAECDIRPAFQHD
                     RDRITHSKSFRRLKYKTQVFLSPTGDHYRTRLTHTLEVSQIARTIGRALGLNEDLVEA
                     IALAHDLGHTPFGHSGEAVLNTLHPGGFRHYEQSLRVVDILEKDGEGLNLTMEVRDGI
                     VKHSKGKGDIIIRDDDERPLTKEAEVVRIADIIGYLNHDIDDAVRGNVISEEDIPRHL
                     RDNLGHSVSDRIDTMVRGVIWSTLQSGRMELGCGEELETHMLEMRDFLYVRVYESPVV
                     HADFIKCSKIIEDLYGYFLKNPDAFLKETDRNDFHDDPVACVRDFIAGMTDRYAFSLF
                     ERIFLPMPWSIPV"
BASE COUNT          630 a          849 c          822 g          579 t
ORIGIN      
        1 gatcatgccg tgagacagag tttatcaaca ggctgttgaa tgttaaagaa cctcgtagga
       61 ccggacggtt cgtccgcccg gcaaagccgg gaccagagtc ttctccaacc caaccatctc
      121 cagtcgtctt gccttcatac tcagacgatt caagctacgt aagccgcctt tcccgtcttt
      181 tgcctttgac tctcaagcga ttcaagccgc ctctaataca gaaacatcgg tttccccagc
      241 gtgtcgcgga cctttggacg gtacgtgtcc acgtcgtaca gggccgtcac atccacctgc
      301 ttcctggccg cgacgaccat atccagcggt acggtggtgt atatcgcctg ctgcagtgcc
      361 accatacggc ccctgtggcc cattgctatc tgatcgagag ccaggctgcc ataggatacg
      421 gcgaccatcc ggtcgaggga atcgggggcc ccggaccgca tcatgtatgc tagctgctgg
      481 tagatgacgt ttacacccga gcgctgcttg atctcctggg aaacgaggta ccccaccccg
      541 ccaagcttct tgtggccgta ggcatcctct ataccggtct ccgtcaccgt cccgcccacc
      601 gtatgggccc cttcggagat ggtcatgata gcatagtttg aggggtttct ggatcggtcc
      661 gtcaccagaa aaccgacgag cttgtcgagg tcgaagggca cttccgatat gacggccctg
      721 tcgacgtccg ccaggtaggc ggcgataagc gatgtctcgc cggaattcct gccaaatagc
      781 tcgacgacgc agatgcgttc atgggaaccc gtcgtggtgc gcagggcgtt gatggcgtca
      841 acggaccgtg tcacggccgt cgaaaaacct atgcagaaat cggttccgaa gacgtcattg
      901 tccatggtct tcgggatggc gacaacctgc acgccttcct tgtggagtcg gaccgcgtag
      961 ctcagggtgt cgtccccgcc gatggggatg atggcatcta tcgccaggga ttcgaggacc
     1021 ttcaggatat gcctcgtgca gtcgatcctt cccgatgccg tcgggacccg gtcaccctcc
     1081 tccacgaagg ggggcacctc gtcggcgttc acgtttcccg gattggtacg tgatgtgtga
     1141 aggatggtcc cgccggtccg atcgatggtc ctgaccttgt cgagagtgag cggcatgacg
     1201 agactctgtg ccgtctcggg actgccggga tcgatgcaga gcaaacccat ccatcccctg
     1261 cggataccga ccacctcaat gtctctcgaa tcggctcgcg cgacaacggc ctttatggcc
     1321 acattgaggc cgggaacatc ccctcccccc gtcagcacgg caattctctt cctccctccc
     1381 ataacaacac ctcccatccc cgtcggggtc acgcgttttc cataaagttc tgtatagtcg
     1441 cgaaagaagg gaatgtcaag gaaggaccaa aaaccgttca acggttcagg ggaaagaccg
     1501 cgaagggggg gtcggctgtc aagggggatt cgtacaccgg ccgaaggcgc cggtgacctt
     1561 tttgtctttt actcttgaac ttttgaaccc tggaactctt gaaccttctt tccgaagttc
     1621 aggtcgttga tattgttaca tctttatgct acattaggat gttggttgca gccgaatgaa
     1681 tatccgtgaa agactggaag aacgggaggg gaaggtcctg tcgccctggg cgcagaagag
     1741 cgccgccact cgcggaagga tgcgggaaga ggccgagtgc gacatcaggc ccgccttcca
     1801 gcacgaccgt gacaggataa cccacagcaa gtctttccga aggctcaagt ataagacgca
     1861 ggtcttcctt tcgcccacgg gagaccacta ccggacacgg ctgacgcaca ccctcgaggt
     1921 ttcgcagatc gcgcggacga tcggccgggc gctcggcctc aacgaagacc tcgtggaggc
     1981 catagccctt gcccacgacc tcggccatac gcccttcggc cattccggcg aagccgtcct
     2041 caacacgctc catcccgggg gcttcagaca ctacgagcaa agcctgaggg tcgtggacat
     2101 actcgagaag gacggcgagg gccttaacct gacgatggag gtcagggacg ggatcgtcaa
     2161 acactccaag ggtaaaggcg acataatcat ccgtgacgac gacgaaagac ccttgacgaa
     2221 agaggcggag gtggtccgca tcgccgacat catcggctac ctgaaccacg atatcgacga
     2281 tgccgtgcgg ggcaacgtca tcagcgaaga ggacatcccc cgacacctca gggacaatct
     2341 cgggcacagc gtttcggacc gcatcgacac gatggtgcgc ggcgtcatct ggagcactct
     2401 tcagagcggc aggatggaac tgggatgcgg agaggaactg gagactcaca tgctggagat
     2461 gcgtgatttc ctctatgtcc gggtctatga gagccccgtc gttcacgcgg acttcatcaa
     2521 gtgttcgaag atcatcgagg atctctacgg gtatttcctc aagaaccccg acgcgttcct
     2581 caaggaaacg gaccgcaacg acttccatga cgatcccgtc gcctgcgtcc gggacttcat
     2641 cgccggcatg acggaccgtt acgcctttag tctctttgaa aggatcttcc tgcccatgcc
     2701 atggagcatt ccggtgtagg cggaagaccg gtgaacgtga aagaacaacg accatcaagc
     2761 gcgttttgat aggtacacac ggaaagggag ctgacaatga tggataaggt ttatgacccc
     2821 cacagcatcg aaaagaaatg gtacgccgag tggctggaga agggatactt cacggccagg
//
LOCUS       DAOPOA010000245         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37689_length_2878_cov_5.055969, whole genome shotgun sequence.
ACCESSION   DAOPOA010000245 DAOPOA010000000
VERSION     DAOPOA010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37689_length_2878_cov_5.055969"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2878)
                     /locus_tag="PKJ17_06015"
     CDS             complement(<1..>2878)
                     /locus_tag="PKJ17_06015"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOD75563.1"
                     /translation="EVISFIRETQSDKWDYLCNIQKDKAEETLLDDLCRALNSEHEGC
                     LSVLRRGFKCFGKEFRVAFFAPASGMNPDTLKAYEMNRLAVTRQLAYSPKHENKIDVT
                     LSVNGIPIVTAELKNQMTGQNYRHAMTQYRKDRDQKDLIFTFKKRSLVHFAVDTDEVY
                     MTTRLSGDKTYFLPFNKGSNGGAGNPESPDGYKTSYLWEQILERHSLLDILARFIHLE
                     VKEKQLGGRTVKTETMIFPRYHQLDCVRQLVDDARRDGVGKNYLIQHSAGSGKSNSIA
                     WLAHRLASLHNDKDEKVFDSIIVITDRIVLDQQLQNTIYQFEHKQGVVRKIGVDSTQL
                     AEALGSGVPIIITTLQKFPYAAGKIDGMKKRSYAVIVDEAHSSQGGETAAEMKGVLAA
                     DAIKEQVKKKAEEEGLNDYEQEIFASMAKRGQQSNISFFAFTATPKHRTISVFGTPGP
                     DGKPAARHLYSMRQAIDEHFILDVLKHYTTYKTYHRLVKSIEDDPEVDKRKAASALAR
                     FVSLHPHNIAQKTEVMIEHFRHFTMHKIGGRAKAMVVTSSRLHAKKYKEAFDKYIEDK
                     GYKGIKTLVAFSGTLVDPDLPELTFTEVGMNGGEIGEKELPKIFGTELYQVLIVAEKY
                     QTGFDQPLLHTMYVDKRLADIQAVQTLSRLNRTCAGKEDTFVLDFVNEPQDIQAAFQP
                     YYEETILGDETTAGQLYDLQAKLGEKQVYHRDEVEEFAKVFFTPLQKQTPADHGKMNK
                     CIDPAVTRFTKLSDDDQEEFRKALIAYRNLYAFLSQIIPFQDSDLEKLYAYIRFLITK
                     LPRGSQGPVYNLDDDVALKFYQLQKIGEKSITLTSGEASPVSGPTSVGTGTAGGPVIE
                     LSALIDILNEKFGTEFKPGDQLFFDSIKEDAVSTANLKQAAIVNTLDGFSYVFRKALE
                     DLFIDRMEQNEDITAKFLNEPKFREEVSKYLEKEVYKQIRDTGG"
BASE COUNT          489 a          807 c          851 g          731 t
ORIGIN      
        1 tcccctcctg tatcgcgtat ctgcttgtag acttcctttt ccagatactt gctgacctct
       61 tcacggaatt tcggctcgtt caggaacttc gcggtgatat cctcgttctg ctccatgcgg
      121 tcgatgaaca ggtcctcaag ggccttcctg aacacgtaac tgaacccgtc gagcgtattg
      181 acgatggcgg cctgcttcag gttcgccgtg gaaacagcgt cttcctttat ggaatcgaag
      241 aagagctggt ccccgggctt aaactcggtg ccgaacttct cgttcaggat atcaatgagt
      301 gctgaaagct ctatcaccgg tccgccggcc gtccctgtac cgaccgatgt cgggccggag
      361 accggggacg cctcgccgga tgtcagcgtt atactcttct ccccgatctt ctgcagttga
      421 tagaatttca atgccacatc gtcatccagg ttatacaccg gtccctggct gcccctgggg
      481 agctttgtga tcagaaagcg tatgtatgcg tagagctttt ccaggtccga gtcctggaag
      541 gggatgatct gtgacagaaa ggcgtacagg ttgcggtatg cgatcagggc cttgcggaat
      601 tcctcctggt catcatcgct gagcttcgtg aacctcgtga cggccgggtc gatgcatttg
      661 ttcatcttcc cgtggtccgc aggcgtctgc ttttggaggg gcgtgaagaa gaccttcgcg
      721 aactcctcca cctcatccct gtggtagacc tgcttctctc caagcttcgc ctgaaggtcg
      781 tagagctgcc ccgcggtcgt ctcatccccg aggatcgtct cctcgtaata gggctggaag
      841 gcggcctgga tgtcctgcgg ctcgttcacg aagtcgagca caaacgtgtc ctccttcccc
      901 gcgcaggtcc tgttgagcct cgagagggtc tggacggcct ggatgtccgc cagcctcttg
      961 tcgacgtaca tggtgtgaag gagcggctgg tcgaacccgg tctggtactt ttcggcgacg
     1021 atcaggacct ggtaaagttc cgttccgaag atcttcggga gttccttctc gcctatctcg
     1081 cccccgttca tgccgacctc ggtaaatgtc agctccggaa ggtcggggtc taccaatgtt
     1141 cccgagaagg cgacgagcgt ctttatcccc ttgtagcctt tgtcctcgat gtacttgtcg
     1201 aaggcctcct tatacttttt cgcatggagg cggctcgagg tcacgaccat tgccttagcc
     1261 ctgcccccga tcttgtgcat cgtgaaatga cggaagtgct cgatcatgac ctccgttttc
     1321 tgggcgatgt tgtgtgggtg caggctcacg aaccgtgcga gggcgctggc ggcctttctt
     1381 ttatcgacct cggggtcgtc ctctatggac ttcaccaggc ggtggtaggt cttgtacgtc
     1441 gtatagtgct tgaggacatc gaggatgaag tgctcgtcga tcgcctggcg catgctgtac
     1501 aggtggcggg cggcaggctt gccgtccggc cccggcgtcc cgaagaccga tatcgtcctg
     1561 tgtttcgggg tggcggtgaa cgcgaagaag cttatgttcg actgctggcc gcgcttggcc
     1621 atggacgcga agatctcctg ctcgtaatca ttcaagcctt cctcttcggc tttcttcttc
     1681 acctgctctt ttattgcgtc ggcggcgagg acgcctttca tctcggcggc cgtctctcct
     1741 ccctgggagc tgtgcgcctc gtcgacgatg accgcgtaac tgcgcttctt catgccgtct
     1801 atcttgccgg cggcgtaagg gaacttctgc agggtcgtga tgatgatggg aacacccgac
     1861 cccagggcct ccgcgagctg ggttgaatcg acgcctatct tccttacgac gccctgtttg
     1921 tgttcgaact ggtagatggt gttctggagc tgctggtcga ggacgatacg gtccgtaatg
     1981 acgatgatcg agtcaaagac cttttcgtct ttgtcgttgt gaaggctcgc caggcggtgc
     2041 gccagccagg cgatcgagtt cgatttgccg ctgcccgcgg agtgctggat taggtagttc
     2101 ttcccgacgc cgtccctgcg ggcgtcatcc acgagttggc ggacgcagtc gagctggtgg
     2161 taccggggga agatcatcgt ttccgtcttg acggtccttc cgccgagctg tttctccttg
     2221 acctcaaggt gaatgaagcg ggccaggatg tcgagaagac tgtgcctctc gagtatctgt
     2281 tcccagagat atgaggtctt gtagccgtcg gggctctcgg ggtttcccgc gccgccgtta
     2341 ctgcccttgt tgaacggcag gaaatatgtc ttatcaccgg acagacgggt tgtcatgtac
     2401 acctcgtcgg tgtccacggc gaaatgaacg agggagcgct tcttgaatgt gaagatgagg
     2461 tccttctggt cacggtcctt cctatactgt gtcatggcat ggcggtagtt ctggcccgtc
     2521 atctggttct tgagctccgc ggtaacgatg gggatgccat tgacggatag agtcacatcg
     2581 atcttgttct catgctttgg agaatacgca agttgccggg tgaccgcgag gcggttcatc
     2641 tcgtaggcct taagcgtatc cgggttcata ccgctcgcag gggcgaagaa ggcgacccgg
     2701 aactccttgc cgaagcactt gaagccccgg cgcaggacgg acaggcaccc ctcgtgttca
     2761 gagttgagag cgcggcagag gtcatcaagg agcgtctctt cggccttgtc cttctggatg
     2821 ttgcacaggt aatcccattt gtcggactga gtttccctga tgaaggatat gacctcct
//
LOCUS       DAOPOA010000246         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37720_length_2877_cov_4.365698, whole genome shotgun sequence.
ACCESSION   DAOPOA010000246 DAOPOA010000000
VERSION     DAOPOA010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37720_length_2877_cov_4.365698"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKJ17_06020"
     CDS             complement(<1..646)
                     /locus_tag="PKJ17_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD75564.1"
                     /translation="MTREGYAVDATESGDTAVSMIKDTVYDLVITDLKLPGIDGMDVL
                     RSVQKLARDTSVIIITAYATVDTAVEAMKEGAEDYIAKPFNLEEIRLVVRKVLDKKAL
                     VDDNRFLRNQLKKKYSFGNLVGNSEAMVEVYKTIEKIKDSRATILILGETGTGKELVA
                     RAIHFNSIRSEKPFLPVNCAALAETLLESELFGYVKGAFTGAVRDKQGVFEVAHG"
     gene            complement(709..1689)
                     /locus_tag="PKJ17_06025"
     CDS             complement(709..1689)
                     /locus_tag="PKJ17_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOD75565.1"
                     /translation="MGSNGVKSKVVLMVLIAGTVPVVLFSLAVFAIDSGPGRLAVTVA
                     FVLLMAAGSYITARRVIELLHSSYEREKELEMQIIKKDKLAAIGLLTAGIAHELNTPL
                     ASALLNTQMLKEDAKKEWPDRTPVLDSIEEEIKRAGSVVRNILDFSRQTQVQSAVTDV
                     NSVLTKLLDISSKLCSEKGILIRRDLHPGIPLARGNGSILHQVFMNIVSNAIEAMDNG
                     GTLSVFSRFLPGQHKVVVDIEDTGPGISREHIDGVFDPFFTTKASEDGTGLGLAISYS
                     MVRKMGGDIRVVSSSDERENFPSTKGTIFSIELPALEEDSPRNQKAGDQD"
     gene            1917..>2877
                     /locus_tag="PKJ17_06030"
     CDS             1917..>2877
                     /locus_tag="PKJ17_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOD75566.1"
                     /translation="METNRTNVEPKDAWSLYPTDILFQTPFWSEVKLQLGWKTHAFDY
                     RASGVDGDILILTRHLGKGIHAAYVQQGPENGPHLEEYGPFLEDLSEALVKHIGKDLA
                     FIRYDLPWPSQYGGNGTEGKLYPDGSIRPEPRLQEVRMNFGTRSWNLRKAAVDMTVAD
                     AVVIDLAQSEEELLGGMKPKTRYNIRLAGRRGVEVVCSDILELPVFYDLYRQTSERNR
                     FDACSYSYFSALYSALASTPDSSEVHFLLARHGRNVLAGAIITISGRTATYLFGASSS
                     QSRNMMAPYAIQWAAIRLARTKGCLTYDMGAVAPAPNLEHPFFG"
BASE COUNT          595 a          851 c          777 g          654 t
ORIGIN      
        1 cgccgtgggc cacttcaaag acgccctgct tgtcccgcac ggcccccgtg aaagcaccct
       61 tcacatagcc gaaaagctcg ctttcgagga gcgtctccgc cagcgccgcg cagttgacgg
      121 gcaggaaggg tttctcggac ctgatgctgt tgaagtgtat ggcccgcgcg acaagctctt
      181 tcccggttcc cgtttcacca aggataagta tggttgccct ggagtccttg atcttttcta
      241 tggtcttgta gacctcaacc atagcctcgc tgttgcccac aaggttgccg aagctgtact
      301 tctttttcag ctggttcctg agaaagcgat tgtcgtcgac aagggccttc ttgtcgagga
      361 ccttccgcac gacaagacgg atctcctcaa ggttgaaggg ctttgctatg tagtcctccg
      421 ccccttcctt catcgcttcg accgccgtgt cgacggtcgc ataggcagtg atgatgataa
      481 cactcgtgtc ccgcgccagt ttctggaccg accgcaggac atccatgccg tctatccccg
      541 gaagtttgag atcggttata acgaggtcat agaccgtgtc ctttatcatc gacacggccg
      601 tatcgcccga ttcggttgca tcgaccgcgt atccttcgcg ggtcataatc tccttcaagg
      661 catgtctgag cttcgtgtcg tcttcaacaa taagaacgct tgccgggttc aatcctgatc
      721 acctgccttt tgattgcgtg gactgtcttc ctcgagcgcc ggaagttcaa tgctgaagat
      781 tgtgcccttt gtgctgggga agttctccct ttcatcactg gagcttacca ctcttatatc
      841 gccgcccatc ttccttacca ttgagtagct tatggcgagt cccagcccgg taccgtcttc
      901 cgacgccttt gtcgtaaaga atgggtcgaa aacaccgtct atgtgctccc gcgatatgcc
      961 cgggcctgta tcctctatgt cgaccacgac cttatgctgc cccggcagga acctggagaa
     1021 tacgctcagg gtaccgccgt tatccattgc ttcgatcgca tttgagacaa tattcataaa
     1081 tacctggtgc agtatgcttc catttcccct tgcaagcgga atgccgggat gaagatcgcg
     1141 gcggatcagg atgcctttct ccgaacaaag cttggaggat atgtccagga gcttggtcaa
     1201 cacggaattg acgtcggtta cagcggactg cacctgtgtc tggcgagaga aatcgaggat
     1261 gtttctgacg accgatcccg ccctttttat ctcttcctcg atggaatcga ggaccggtgt
     1321 tcgatccggc cattcctttt tggcgtcttc cttcagcatc tgggtattga gaagagcgct
     1381 cgcgagtggc gtgttgagtt cgtgcgcgat gccggccgtc agaaggccga tggccgccag
     1441 cttatccttc ttaatgatct gcatctcgag ttccttttcc cgctcatagg aactgtggag
     1501 gagctcgatc accctgcgcg ccgtgatata gctgcccgcg gccatcagaa gcacgaaggc
     1561 caccgtgacc gccaggcgcc cgggacctga atcaatcgca aacaccgcga gcgagaacag
     1621 aacgaccggc accgtgcccg ctatcagcac catcaaaacg acctttgact tgaccccgtt
     1681 tgaacccatg atatgacctc gagtaatgat ccgttccatt ataacataga taggtgcccg
     1741 ccgttcctga caggcaagaa cgatgccact cttgactggc cccgggtttg catatggcaa
     1801 ggcgggtcgc ggtcgtttta tccgttcgga aagacagcgg agcggcccgc ttcccgggaa
     1861 ggaagtccat cttccttccc ggacctatgt cgccagcagg aggttgtgtc agtgcaatgg
     1921 aaacaaatcg aacaaatgtc gaaccaaagg acgcatggtc actctacccg actgatattc
     1981 tcttccagac cccgttctgg agtgaggtaa agctccagct tggctggaag acgcatgcgt
     2041 tcgattacag agcttcgggc gtagacgggg acatcctcat ccttacccgg catctgggta
     2101 aagggataca tgccgcctac gttcagcagg gtccggagaa cggtccccat cttgaagaat
     2161 acggtccctt cctggaggac ctgtctgaag ccctcgtgaa gcatatcggg aaggacctgg
     2221 ccttcatccg gtacgacctc ccctggccgt ctcagtacgg cggcaacgga acggaaggaa
     2281 agctatatcc ggacggctcg atccggcccg agccaaggct ccaggaggtc cgcatgaact
     2341 tcggcacgcg gtcatggaac cttcgcaagg cagccgtcga catgaccgta gccgatgccg
     2401 tggtaatcga cctcgcgcag tccgaagaag agctcctcgg gggtatgaag ccgaagaccc
     2461 gatataacat acgcctcgcc gggagaaggg gcgtagaggt ggtgtgttcc gacatcctgg
     2521 agttgcccgt tttctatgac ctgtaccgcc agacgtcgga acgcaacaga ttcgatgcct
     2581 gcagttacag ctatttctcc gcgctatact ccgccctggc ttcgacgccg gactcatcgg
     2641 aggtccattt cctgctggca aggcacggga ggaatgtgct ggcaggtgcg atcatcacca
     2701 tctcggggag gacggcaacg tacctgttcg gagcatcctc cagtcagagc cggaacatga
     2761 tggcgcccta cgccatccaa tgggccgcca tccgccttgc ccggacgaag ggctgcctga
     2821 cctatgacat gggagcggtt gcgccggcac caaacctcga acacccattc ttcggaa
//
LOCUS       DAOPOA010000247         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37722_length_2877_cov_3.148122, whole genome shotgun sequence.
ACCESSION   DAOPOA010000247 DAOPOA010000000
VERSION     DAOPOA010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37722_length_2877_cov_3.148122"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..924
                     /locus_tag="PKJ17_06035"
     CDS             250..924
                     /locus_tag="PKJ17_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD75567.1"
                     /translation="MLLSVIIPAYNEIEFIDEILRKVKETPYDKEIIVVDDCSTDGTR
                     EYLRVQKGIVTIFQERNQGKGAALRRGIAEATGDIVLIQDADLEYDPREYPVLLGPIL
                     EGKADVVYGSRFLGGPHRVLYFWHFVGNSAVTLLSNMFTNLNLTDMETCYKVFRKEVI
                     KAITIESDRFGVEPEITAKVAKGKWRIFEVPISYYGRTYEEGKKITWRDGIKAFFTIV
                     KYNVFR"
     gene            complement(1282..2835)
                     /locus_tag="PKJ17_06040"
     CDS             complement(1282..2835)
                     /locus_tag="PKJ17_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HOD75568.1"
                     /translation="MKDGDAQQKGVSRRSLLKSAAAVGAVAGLSGMALNLAAPGSAEA
                     VTKKLPQKWDETWDVVVIGSGFAGLAAAAEAAGSGAKTVILEKMPIYGGNSIINGGGY
                     AAWDEELHLRKKKNLGEDSVKQHIQDTLKGGDFYNMPELVEIMAKEAPDALNWMINQG
                     GAELSDVLVIGGGHTAYRAHFSKHNSGKDYTEALKKIAEKNGAKMVLNSQVTWIWRKD
                     ADPKSPVLGVEVKRGNKIVNIKAKKALILASGGFSNDVEMRLSEWPKLVKEFNCTNQK
                     GATGEMIRYAKAVGSDALHMNFIQLFPFAEPEAGILDRPAVYAFSGPGVGIIYVSKAG
                     KRFVNELDRRDVCAFAQIALGTKPAYAIFTDAIPPKFGGKPAEIEAGMKKGRFIKGDT
                     IADLAKKIGAPADVLEKTINDHNKYIAEGKDAEFNKPMKKTMVPMDKGPYYALPMWPA
                     VHHTMGGLRINTNSQVIDVFGAVIPRLYAAGEVVGGVHGSNRLGCNATTECVVFGRIA
                     GKNGAKEKA"
BASE COUNT          600 a          799 c          802 g          676 t
ORIGIN      
        1 acaatgaagt actccctcgt accggtacga aaaaaataat tgcgacacag tctcttcgcc
       61 ggaatgacag cacgtgagga aggaatgacg ggcggagagg ggtggcggga tgacgaaaca
      121 tatgaggccg caccgatgag ggttggccca acgtttctgc gcacaggatt ccgattcatt
      181 gctaaaaccc acaaccatgc ttctcttttc attcttgaat ttttccaggt gtccatataa
      241 cattacgcta tgctcctatc cgtcatcata ccggcctaca atgaaataga gttcatagat
      301 gagatcctcc ggaaggtgaa agagacgccc tacgacaagg agataatcgt tgtcgatgat
      361 tgctcgacgg atgggacacg ggaatatctg agggttcaga agggcatcgt caccatcttt
      421 caggagagga accagggcaa gggagcggcc ttgaggcgcg ggatagctga ggcaacaggc
      481 gacatcgtcc tcattcagga cgccgacctg gaatacgacc ccagggagta cccggtcttg
      541 ctcggcccca tccttgaggg aaaggccgac gtggtctacg ggtcgcgttt cctcggcggg
      601 ccgcacaggg tgctctactt ctggcatttc gtgggcaatt cggcggttac gctcctgtcc
      661 aacatgttca ccaacctcaa cctcacggat atggagacct gctacaaggt tttcaggaaa
      721 gaggttataa aggccataac gatcgaatca gaccgcttcg gcgtggagcc cgagatcacc
      781 gcgaaggtcg cgaaggggaa atggcggatc ttcgaggttc cgatctccta ctacggaagg
      841 acctacgagg aagggaagaa gatcacctgg cgcgacggca tcaaggcgtt cttcacgata
      901 gtcaagtata acgtgttcag gtgaaagagg gcttgaatcg cttgagcggc ttgaatcgcc
      961 tgacagattg ttggcaaagt ccgctcctcc gtcattccgg cgaaggccgg aatccaggaa
     1021 aaagtctgtc gcagcagaaa gcacgcaaaa gataataccc ttaagagtcc ggtatgagag
     1081 atgccgcgga acccgttcct gtgaaatggt cgttcgctga cagtctttcc tggattccgg
     1141 ccttcgccgg aatgacgaga gagcacgaac agccttgaga aatcactttg tcaacagtct
     1201 gttgagggtt agaggccaaa gacgaaggca aatgaaaagg gccatctccg gaaggggatg
     1261 gcccttgtgt tcttgtgctc gttatgcttt ttccttggct ccgttcttgc ctgcgatacg
     1321 gccgaagacg acgcattcgg ttgtggcgtt gcagcccaga cggttcgaac cgtggactcc
     1381 tccgaccacc tcaccggcgg cgtacagacg aggtatcacc gcgccgaaga cgtcgatgac
     1441 ctgggagttc gtgtttatcc tcaggccgcc catggtgtgg tggacagccg gccacatcgg
     1501 cagggcatag tagggcccct tgtccatggg gaccatcgtc ttcttcatgg gtttgttgaa
     1561 ctcggcatcc tttccctctg ctatgtactt gttgtggtcg ttgatggtct tttcaaggac
     1621 atcggcagga gccccgatct tcttggcgag gtcggcgatg gtgtcaccct tgatgaaacg
     1681 gccttttttc atgccggctt ctatttccgc tggtttgccg ccgaacttgg gcggtatcgc
     1741 atccgtgaag atcgcatagg cgggtttcgt accgagagcg atctgggcga aggcgcatac
     1801 gtccctgcgg tccagttcgt tgacgaaacg tttcccggcc ttgctcacgt agatgatccc
     1861 gacgcccgga ccgctgaaag cgtatacggc aggcctgtcg aggatgcctg cttcgggttc
     1921 ggcgaagggg aagagctgta tgaagttcat gtgcagcgcg tccgatccta cggccttggc
     1981 atagcgtatc atctcgcccg tcgcaccctt ctggttggtg cagttgaact ctttgacaag
     2041 cttgggccat tctgaaagcc gcatttccac atcgttactg aagcctcccg aggcaaggat
     2101 gagggccttc ttggccttta tgttcacgat cttgttgcct cttttgacct caaccccgag
     2161 gacaggactc ttgggatcgg cgtccttacg ccagatccag gtgacctggg aattgagcac
     2221 catctttgcc ccgttcttct cggcgatctt cttgagtgct tcggtatagt ccttgccgga
     2281 attgtgcttg gagaagtgag cgcggtatgc tgtatgtccg ccaccgatga cgaggacgtc
     2341 actgagttcg gcgccaccct gattgatcat ccagttgagg gcatccggag cctcttttgc
     2401 catgatctca acgagctccg gcatattgta gaaatcgccg cccttcaggg tatcctggat
     2461 gtgctgcttg acgctgtctt caccgagatt ctttttcttg cgaagatgca gctcttcatc
     2521 ccacgcagca tagccgccgc cgttgatgat ggagtttccg ccgtagatgg gcatcttctc
     2581 gaggatgacg gtctttgctc cgctgcccgc cgcctccgct gctgccgcaa gtcctgcaaa
     2641 ccccgagcct ataacgacaa catcccatgt ctcgtcccat ttctggggca gctttttcgt
     2701 gacggcctca gccgaaccgg gagccgcaag gttcaacgcc atgccgctca aacccgccac
     2761 tgccccgacc gccgcagcgc ttttgagcag agaccgcctg ctcacgcctt tctgttgcgc
     2821 atctccatct ttcatgtctc ctccttgtga tgttccccgc tcccaccgta aaacatc
//
LOCUS       DAOPOA010000248         2873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37782_length_2873_cov_3.435770, whole genome shotgun sequence.
ACCESSION   DAOPOA010000248 DAOPOA010000000
VERSION     DAOPOA010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37782_length_2873_cov_3.435770"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKJ17_06045"
     CDS             <1..187
                     /locus_tag="PKJ17_06045"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75569.1"
                     /translation="DLITSLAPKALIYVSCNPATLARDLGRLASAGYAVELVAPFDLF
                     PHTSHLETLTVLKKRRR"
     gene            184..519
                     /locus_tag="PKJ17_06050"
     CDS             184..519
                     /locus_tag="PKJ17_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75570.1"
                     /translation="MKREKMDRKAEGHEPPERFETIRHYISALLEEGTYTSKELSGII
                     KIPERDVCDHLEHLQRSLSKGDRRLTVISASCRHCGFVFRKRDRLTKPGKCPSCRSIH
                     IQPPMFHIE"
     gene            703..1764
                     /gene="lpxK"
                     /locus_tag="PKJ17_06055"
     CDS             703..1764
                     /gene="lpxK"
                     /locus_tag="PKJ17_06055"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOD75571.1"
                     /translation="MRDTVVRVWNGEAKVARWLLYIPLAFLSYLYHIALMAREYMYRT
                     GIMATHSAPIPVVSVGNITLGGTGKTPVVERLSQRFKDAGYNPGIITRGYMRKRSGTF
                     AVDVRNDSAHTAGDEAYMLARRTRIPVIVGKDRMKAIEQGIEEKNIDMAILDDGFQVR
                     DLRKDVDILILNGRESLSRQELFPLGPYREPVSRVRESDTILVSKSEPDSATLYFTQG
                     IPRFQVRFKPVHLYNVKKNAMAHYRFLRNKKVLAFSGLGDNQSFFQLLRQIGADVVQE
                     IPFPDHHRYTAGDLRRMGSAENVQCIVTTEKDAVKLMGMDVPDNLFYLSIEARIEDED
                     TLMDLLLKKLGTRSPDRLA"
     gene            1893..>2873
                     /locus_tag="PKJ17_06060"
     CDS             1893..>2873
                     /locus_tag="PKJ17_06060"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOD75572.1"
                     /translation="MSEQVFIFDTTLRDGEQSPGNTMNTQEKLRVARQLELLGVDIIE
                     AGFPIASEGDFDAVKQIAKLVKNCQIAGLARANDEDIDRAWKAIKVAAHPRIHTFIST
                     SDIHLKHQFRKTRDEVLEIAVAAVKRAKRYTDNVEFSAMDATRSDWDYLCKVVAEVID
                     AGATTVNIPDTVGYAVPHEFGELIAYIRKNVPNIDKAIISVHCHNDLGLATANSIAAI
                     HNGARQVECTINGIGERAGNASLEEIAMTLRTRKTSFTADTRIVSEKIYPTSRLITSI
                     TGVPVQPNKAIVGANAFAHESGIHQDGLLKAKLTYEIMTPESVGISKSSLI"
BASE COUNT          725 a          859 c          776 g          513 t
ORIGIN      
        1 ggacctcatc accagccttg caccgaaagc gctcatatat gtctcctgca accctgccac
       61 cctcgcccgg gacctcgggc gtctcgcctc ggcaggctac gccgtcgaac tcgtcgcccc
      121 cttcgacctc ttcccccaca cctctcacct ggaaaccctg acggtcctga agaagaggag
      181 aagatgaaaa gagagaagat ggacagaaaa gctgaagggc atgaacctcc cgaacgcttc
      241 gagaccatcc ggcactacat ctcggccctc ctcgaggaag gtacatacac atcgaaggag
      301 ctgtcgggca tcatcaagat acccgaaagg gacgtttgcg atcacctgga acacctgcag
      361 cggtccctca gcaagggcga caggcgtctc accgtgatct cggcctcctg caggcactgc
      421 gggttcgtct tcagaaagcg tgaccgcctc acaaaacccg gcaaatgccc ctcctgccgc
      481 tccatccaca tccagccacc gatgtttcat atagaatagt gttaagtttt aggttttaag
      541 ttttaagtga aacacccggc gaggcagaaa acgttcggtg tggagtctca ccgcggagta
      601 tttgacactt aacacctaaa acttaacact taaaactcta aacggtaacc tatcatcatt
      661 attgcaatta ccgagctttt ctgttatata tttcgattgc ctatgcgtga taccgtggtc
      721 agggtgtgga atggtgaagc aaaagtggca cggtggctcc tttacatacc ccttgctttt
      781 ctctcttatc tctaccacat agcgctcatg gcgagagagt acatgtacag gacggggatc
      841 atggcgacgc acagcgcgcc cataccggtt gtcagcgtgg gaaacatcac cctcgggggg
      901 acggggaaga cacctgtcgt cgagaggctc tcccagaggt tcaaggacgc gggctacaac
      961 ccggggatca taacgcgggg atacatgagg aagaggagcg ggacctttgc cgtggacgtg
     1021 aggaacgact cggcacacac tgccggggac gaggcataca tgctggccag gagaacacgg
     1081 atacccgtca tcgtcggcaa ggaccgcatg aaggccatcg aacagggcat cgaagagaag
     1141 aacatcgata tggccatcct cgacgacggt ttccaggtgc gggacctgag gaaggatgtg
     1201 gacatcctca tcctcaacgg acgggagtcc ttgagcagac aggagctttt tcccctggga
     1261 ccctaccgcg aacccgtgtc acgggtgcgc gagtcggata ccatactcgt gagcaaatcg
     1321 gaaccggaca gcgccaccct ctatttcacc cagggcatcc cccggttcca ggtgagattc
     1381 aagcccgtcc acctctacaa cgtgaagaag aacgccatgg cccactaccg gttcctcagg
     1441 aacaaaaagg tcctcgcctt cagcgggctt ggcgataacc agtcattctt tcaactcttg
     1501 agacagatag gagccgacgt agtccaggaa atacccttcc ccgaccacca caggtacaca
     1561 gcgggtgatc tcaggcggat ggggtcggcg gaaaacgttc agtgtatcgt caccaccgaa
     1621 aaggacgccg tgaagctcat ggggatggat gttcccgaca atctctttta cctctccatc
     1681 gaggcgcgga tagaagatga ggatacactg atggatctcc tgctcaagaa gctcgggacc
     1741 cgttccccgg accgcctcgc ctaaagagga gataaagcgg gtcggggaag aaagctcaaa
     1801 gaaccatctc acacctcgaa cctgcagaca caatagacgg ctgtgcctcc cgccatggcg
     1861 cgggatggaa aatacaaaaa ggaggagaga gcatgtcaga acaggtattt attttcgaca
     1921 cgacattgcg cgacggcgag caatcgcccg gcaataccat gaacactcaa gaaaagctca
     1981 gggtggcacg acagctcgag ctcctcggtg tggacatcat agaagccggc ttccccatcg
     2041 cctccgaggg cgatttcgat gccgtcaaac agatagcgaa gctcgtgaag aactgccaga
     2101 tcgcgggact cgcccgcgcc aatgatgagg acatagaccg ggcgtggaag gcgataaagg
     2161 tagctgccca cccccgtatt cacaccttca tctccacctc ggacatccac ctcaagcacc
     2221 agttcagaaa gacgcgcgac gaggtgctcg agatcgcggt ggccgccgtg aagagggcaa
     2281 agcgatacac cgacaacgtc gagttctccg cgatggacgc gacgaggagc gattgggatt
     2341 acctgtgcaa ggtggtcgcc gaggtcatcg acgccggggc gacaacggtc aacataccgg
     2401 acaccgtcgg ctatgccgtc ccccacgagt tcggggagct catcgcctac atcaggaaga
     2461 acgtccccaa catagacaag gccatcatca gcgtccattg ccacaatgac ctcggccttg
     2521 ccacggccaa ctccatcgct gccattcaca acggcgcgcg ccaggtggaa tgcacgataa
     2581 acggcatcgg tgaaagggcg gggaacgcct ctctcgaaga gatagccatg accttgagaa
     2641 cgaggaagac gagcttcacc gctgacaccc gcatcgtctc cgagaagata tacccgacga
     2701 gcaggcttat aacctccatc acgggcgtgc ccgtgcagcc caacaaggcc atcgtcggcg
     2761 ccaacgcctt cgctcacgag tcgggtatcc atcaggacgg cctcctgaag gcaaagctca
     2821 cctatgagat catgaccccc gaatcggtgg gcatctccaa gagctccctc atc
//
LOCUS       DAOPOA010000249         2872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37794_length_2872_cov_3.277955, whole genome shotgun sequence.
ACCESSION   DAOPOA010000249 DAOPOA010000000
VERSION     DAOPOA010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37794_length_2872_cov_3.277955"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PKJ17_06065"
     CDS             complement(<1..651)
                     /locus_tag="PKJ17_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75573.1"
                     /translation="MKDENLSSKYKWCTLVLVITMTVLLIRLWDLQIMRGSEMRKLSE
                     QNRIRVKKIVAPRGIIYDRTGKVLADTRPSFNIYLTPEDIRDFSQTVDGLARLLTCDR
                     EEIIEKMKAASGMPPSFPIKIKSDIPMDEVARVEAHRVYIPGVSIQIEPKRNYPYGAS
                     FAHVIGYVSEISDEELKNKKKYKDYSPGDYIGKYGLERSYENDLRGVDGEKRVEVDA"
     gene            complement(651..1142)
                     /gene="mreD"
                     /locus_tag="PKJ17_06070"
     CDS             complement(651..1142)
                     /gene="mreD"
                     /locus_tag="PKJ17_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOD75574.1"
                     /translation="MKTLVYIILGIVLLVIEAVISPHISLDVLKPELGMPIVLYVTFF
                     LGARSGLAAAVCIGLVEESLSGAPGGSLLFTTMFIYLIAMAMRRKFFVDSKYSFAYLS
                     SASVVVQSLLFLALTFFARGEARGALNILFYTIPNAIVTGFASILLFSLIEQLNETFL
                     ERN"
     gene            complement(1139..1939)
                     /gene="mreC"
                     /locus_tag="PKJ17_06075"
     CDS             complement(1139..1939)
                     /gene="mreC"
                     /locus_tag="PKJ17_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOD75575.1"
                     /translation="MRNSIAIIIAILVLVISFLSFTNAPFVARSYSTLKGGLGSVFGP
                     VLSVASKPVSAVGGFFDSYFNLVGAKKENEELREKYEKLYVENQKLVETERENARLRK
                     LLDFTQKRPNTMIAAGVIGEDLKNWFRCIIIDKGSKQNVRPKMPVVTPKGIVGQVVEV
                     DLWHAKVMVLNDTNSSIDVNVEGKNTRGLLEGTGQNTVKLKYVIKNDDIEIGDKLVTS
                     GKDGIYPKGIPAGIVITANKNKAGIFADIDVMPYNNFRQLEEVLLIKK"
     gene            complement(1952..>2872)
                     /locus_tag="PKJ17_06080"
     CDS             complement(1952..>2872)
                     /locus_tag="PKJ17_06080"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOD75576.1"
                     /translation="VHKDSRGTKKVIAVGEEAKKMLGKTPGNIVAIRPLKDGVIADFE
                     ITEAMLKYFIQKIHNKKSYARPRIVISVPSGITPVEKRAVKESAESAGAREVYLIEEP
                     MAAAIGVGLPITEPSGNMIVDIGGGTTEVAVISLAGIVYCNSVRVAGDKIDEAVIQYV
                     KRKYNLLIGERTAELIKITIGSAYPSSEEEEMTMEIKGRDLVGGIPKTLEISSKEIRE
                     AINEPVNAIVEAVRIALERTPPELASDIVDKGIVISGGGAMLKNLDVLIKEVTRLPVI
                     IAENPLTAVVEGAGKALDEVSLLKEIATYF"
BASE COUNT          525 a          806 c          831 g          710 t
ORIGIN      
        1 ggcgtccacc tcgacccgct tttccccgtc tacccccctg aggtcgttct cgtaggacct
       61 ttcgagcccg tatttcccga tatagtcgcc cggggaatag tccttgtatt ttttcttgtt
      121 cttgagctcc tcgtcgctta tctccgacac gtagccgatc acgtgggcga atgacgcccc
      181 gtaagggtaa ttgcgctttg gctctatctg tatggaaacg cccggtatgt acacgcggtg
      241 cgcctcgacg cgggcgacct catccatggg gatatcggac ttgatcttga tgggaaagga
      301 agggggcatt ccgctggcgg ccttcatctt ctctatgatc tcttcgcgat cgcaggtgag
      361 aaggcgggca agtccgtcga cggtctggct gaagtccctg atatcctccg gggtcaggta
      421 gatgttgaag gaggggcgcg tgtcggcaag gaccttgccg gtcctgtcgt agattattcc
      481 ccgcggagcg acgatcttct tgaccctgat gcggttctgc tccgacagtt tgcgcatctc
      541 cgagccgcgc attatctgca ggtcccacag ccggatgagg aggaccgtca tggttatgac
      601 gaggacgagg gtgcaccatt tgtacttgct cgataggttc tcgtccttca tcaattcctc
      661 tccaggaagg tctcgttgag ctgttcgatg agggagaaga gcaatatgga cgcgaacccg
      721 gtgacgatgg cgttggggat ggtgtagaag agtatgttga gcgcaccccg ggcctcaccg
      781 cgggcgaaga aggtgagggc caggaaaagc agggactgca caacgacgga cgcgctggag
      841 aggtacgcaa agctgtactt ggaatcgacg aagaacttcc tgcgcatcgc catggcaatg
      901 aggtagatga acatggtggt gaacaggagc gacccgccgg gagcccccga aagactctct
      961 tccacgaggc cgatgcacac cgcggcggcg agccccgatc tcgctcccag gaagaaagtg
     1021 acgtagagca cgatgggcat ccccagttcc ggcttgagga catcgagaga gatgtgggga
     1081 gagatcaccg cttcgatgac gagaagaacg atgccgagga tgatgtaaac aagggtcttc
     1141 atttctttat gagcaggact tcttcaagct gcctgaagtt gttgtacggc atgacgtcga
     1201 tatcggcaaa gatcccggcc ttgttcttgt tggccgtgat gacgatcccc gcggggatcc
     1261 ccttggggta gatcccgtcc ttgccggagg tgacgagttt gtcgcctatt tcgatatcat
     1321 cgttcttgat gacgtatttc agcttgaccg tgttctgccc cgtaccttcg agaagcccac
     1381 gggtattttt gccctcgacg ttgacgtcta tcgacgagtt ggtgtcattg agcaccatga
     1441 ccttcgcgtg ccagaggtcc acctcgacga cctgccccac tatgcccttc ggcgtcacca
     1501 cgggcatctt tggcctgacg ttctgcttgc ttcccttgtc gatgattatg cagcggaacc
     1561 agtttttgag gtcttccccg atgacgccgg ccgctatcat cgtgttcggt cttttctgcg
     1621 tgaagtcgag gagttttcga agccgggcgt tctccctttc cgtttcgacg agcttctggt
     1681 tctcgacgta gagcttctcg tatttttccc ggagctcctc gttctccttc ttggctccga
     1741 cgagattgaa gtaggaatcg aagaaccctc ccacggcact gacgggcttg ctcgcgacgc
     1801 tgagcaccgg cccgaagacg ctgccgaggc cgcctttcag cgtggaatag gaccttgcca
     1861 caaaaggcgc gttggtaaat gagaggaatg atatgacaag aacgagaatg gctatgatta
     1921 tagctatcga gtttctcaag tcgccactat cttagaaata tgtcgctatc tctttcagaa
     1981 ggcttacctc gtcaagtgct tttcctgcgc cttccacaac agccgtcagg gggttttcgg
     2041 caatgatgac gggaagacgg gtaacctcct tgatgaggac gtccaggttc ttgagcatcg
     2101 cccctccgcc gcttatgacg atacccttgt cgacgatgtc cgacgcgagt tccggcggtg
     2161 tcctttccag ggctatcctg acggcctcga cgatggcgtt caccggttcg ttgatggcct
     2221 cccggatctc cttggacgat atctccagcg tcttcgggat gccgcccacg aggtctcttc
     2281 ccttgatctc catggtcatc tcttcctctt cggaactcgg gtatgccgat ccaatggtga
     2341 tcttgatcag ctccgccgtt ctttcgccga tgagcaggtt gtacttgcgt ttgacgtact
     2401 ggatgacggc ctcgtcgatc ttgtcgcccg caacgcgtac ggagttgcag tacacgatgc
     2461 cggccaggct tatgacggcc acctccgtcg tgcccccgcc gatgtcgacg atcatgttgc
     2521 cgctcggctc cgttatgggc aggccgacgc ctatggcggc ggccatcggt tcctctatca
     2581 ggtagacctc gcgcgctccg gcggactccg cggattcctt tacggccctc ttttcaacgg
     2641 gtgtgatacc cgagggaacg gagatgacga tgcgcggtct ggcgtaggat ttcttgttgt
     2701 gtatcttctg gatgaagtac ttgagcattg cctcggtgat ctcaaagtcg gcgatgacgc
     2761 cgtccttgag cggcctgatg gcgacgatgt tgccgggggt cttcccgagc attttctttg
     2821 cctcttcacc gacggctatg accttcttcg tgccccgtga gtccttgtgg ac
//
LOCUS       DAOPOA010000250         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37850_length_2868_cov_3.858159, whole genome shotgun sequence.
ACCESSION   DAOPOA010000250 DAOPOA010000000
VERSION     DAOPOA010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37850_length_2868_cov_3.858159"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..351)
                     /locus_tag="PKJ17_06085"
     CDS             complement(49..351)
                     /locus_tag="PKJ17_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOD75577.1"
                     /translation="MSRPRKCRCVCCKLECDYFKPRGTPRTELVEVCLRIDELEALRL
                     ADVEGLYQEEAARQMNISRATFGRIVEAAHRKVADAILHGKALRIESEEAATGGRI"
     gene            complement(459..2480)
                     /locus_tag="PKJ17_06090"
     CDS             complement(459..2480)
                     /locus_tag="PKJ17_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOD75578.1"
                     /translation="MVVEYLQAMVIVLGISALIVFVLGKLRISSVVGFLLAGILIGPY
                     AFNLVGDPHEIELLAEIGVVLLMFTIGLEFSLKNLFQLKSIVLGGGAMQVALTVAFVV
                     CIGYFFYTQPLEGALFDGFLVALSSTAIVFKILLDRAQMNSPHGRFSVGILIFQDLCV
                     VPFMLLIPVLAGNSGGGSIALTIAKAALVIALILFASRWFVPFIFKQVVGMKSRELFV
                     ISIITMCLGTALLTSSMGLSLALGAFLAGMVISESEYAAQAVSDVLPFKESFTGLFFI
                     SVGMLLDLRVLASNIVGVSAMVFVIVILKVTAITITGMVLGNSLKNSLMTGLHLFQIG
                     EFSFILALEGKKHGLITNDLYQAFLSASIITMIMTPFFIRSSSSIAEWIARKSPLRLL
                     EKRRGRAGSERYPESIRDHVIIIGFGLNGGNLARVLRSLDIRYVTLELNSRTVRAGKR
                     KGEPIYYGDGTSAEILHKIGVRHASVLVVAISDAPSTRRIVQLARSSNPHLYIIARTK
                     YVAEVEGLKHLGANDVIPEEFETSVEIFSKVLNRLQVPVNDIREHIDRIRQDNYLALR
                     VVKLPRRYLVEREDILKNIVIETYRIRQGSVADEKTLRDLNLRSKTGVTVIGIERAGE
                     IHRMPSPSLRLMADDVLLFIGSREDIDCAVDYLDSNENEPPQCSIREDT"
     gene            2673..>2868
                     /locus_tag="PKJ17_06095"
     CDS             2673..>2868
                     /locus_tag="PKJ17_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75579.1"
                     /translation="MVEKWIEEIKRSVDPGELGMMLVHNGVVRATSKEGKPVKGMRLS
                     YDRALLAAAVEEVKKRDGIAD"
BASE COUNT          662 a          751 c          849 g          606 t
ORIGIN      
        1 aaacggtgct ttcaacgcct tcgtctttca agaccgcaaa gcatatcttc atatccttcc
       61 tcctgttgct gcttcttccg attctatcct cagggccttg ccgtgaagga tggcgtcggc
      121 gactttccgg tgtgccgcct ccactatcct gccgaaggtt gcccgggaga tgttcatctg
      181 cctcgccgct tcttcctgat agagcccttc gacatccgcg aggcggaggg cctccagttc
      241 gtcaattctc aggcagacct cgacaagctc cgtgcggggt gtgccgcggg gtttgaaata
      301 gtcacactcc aacttgcagc acacgcacct gcatttcctc ggccgagaca tggatgtata
      361 ataagcatat gctcaaaaca agtcaagggg gtacaaaccg ttacaggttt caggtgcaag
      421 gcaaacgaaa taccatcggt cgacggaaag gaagtttttt aagtatcctc tcttattgag
      481 cattgcgggg gctcgttctc attggaatcg aggtaatcga cagcgcagtc gatgtcttcg
      541 cggctgccga tgaaaagcag gacatcgtcg gccataagac gcagcgaagg agagggcatc
      601 ctgtggatct cgccggcacg ttcgatgccg atcacagtta cgcctgtctt tgaccggagg
      661 ttgagatccc tgagggtttt ttcatcggcc accgagccct gccgtatccg gtaggtctcg
      721 atcacgatgt tcttgagtat gtcctcgcgt tcgacgaggt atctcctggg gagcttcaca
      781 accctcaatg caaggtaatt gtcctgccgt atcctgtcga tgtgttcccg gatatcgttg
      841 acggggacct gcagccggtt cagcaccttg gagaagatct ccacggaggt ttcgaattcc
      901 tcgggtatga catcgttggc tccaagatgc ttcagtcctt ccacctcagc cacgtacttc
      961 gtgcgggcaa tgatgtacag gtgagggttc gacgaccgtg ccagctgcac tatcctcctc
     1021 gtggacgggg cgtcgctgat tgctaccacg aggacacttg cgtgccgaac ccctatcttg
     1081 tggaggatct ccgcgctggt tccgtcgccg tagtagatgg gctcaccctt ccttttccct
     1141 gcccggacgg tcctgctgtt gagctcgaga gtcacatatc tgatatccag ggaacggagg
     1201 acacgcgcga gattaccgcc gttgagaccg aagccgatta tgatcacgtg gtcccttatg
     1261 ctctcgggat agcgttcgga gcctgctctg cctcttcttt tttccaggag gcgaagaggg
     1321 cttttcctcg cgatccactc tgcgatggac gacgagcttc ttatgaaaaa aggcgtcatg
     1381 atcatggtga tgatcgaagc cgacaggaag gcctggtaaa gatcgttggt gatgaggccg
     1441 tgcttttttc cttccagggc gaggatgaac gagaattcgc cgatctgaaa gagatggagg
     1501 cccgtcatga gagagttctt gagtgagttg ccgaggacca tgccggtaat ggttatggcg
     1561 gttaccttga gtatcactat cacaaagacc atcgccgaaa cgccgacgat gttgcttgca
     1621 agcaccctga gatcgaggag catgccgacg gagatgaaga agagccccgt gaagctctcc
     1681 ttgaagggaa gaacatcgga aacggcctga gccgcatact ccgattccga gatgaccatg
     1741 cccgcaagga aggcgccaag ggcaagggac aggcccatgg acgaggtgag cagcgccgta
     1801 ccgaggcaca tcgtgatgat ggatatcacg aagagctccc ggcttttcat tcccacaacc
     1861 tgtttgaata tgaacggcac gaaccaccgg gaggcaaaga ggatgagagc tatgaccagt
     1921 gcggcctttg caatggtgag ggctatgctg ccgccgcccg aattgccggc aagaacgggg
     1981 atgaggagca tgaagggcac aacgcagagg tcctggaaga tgaggatgcc cacggaaaag
     2041 cgaccgtggg ggctgttcat ctgagcgcga tcgaggagga tcttgaagac gatcgccgtg
     2101 ctgctgagag ccacgagaaa accatcgaac agcgctccct caagcggctg ggtgtagaag
     2161 aaataaccta tgcagacgac aaaggcgacg gtaagcgcga cctgcatggc tccgccccca
     2221 agaacgatgg acttcagctg gaagaggttc ttgagggaaa actcgaggcc gatggtgaac
     2281 atgagcagga cgaccccgat ctcggcgagg agttctattt cgtgagggtc gccgacgagg
     2341 ttgaaggcgt acggtccgat gaggataccg gcaaggagga atcccaccac ggatgatatc
     2401 cggagcttcc cgagaacaaa cactatgagg gcgcttatcc cgaggacgat caccatcgcc
     2461 tgaagatatt caactaccat ccgttccttt ctatccgagc atgcaatcgc atatgccggg
     2521 ccgcgtcacg acgcctggcg atccttatat aatacgccga tttgcccgga aaaccaaggg
     2581 gttctctcga aaacggccgg cgggaatccg ggacggaaac cgcttcttga ttttccttcc
     2641 cgtggatggt agaaaccggt aggaggaaga cgatggttga aaaatggatc gaagagatta
     2701 agagatcggt ggacccgggt gaactcggga tgatgctcgt gcacaatggg gtcgtcagag
     2761 cgacctcgaa ggaagggaaa ccggtgaagg gcatgcggct ttcctatgac agggcgctcc
     2821 tcgccgccgc ggtggaagag gtgaagaaac gtgatggaat agcggacg
//
LOCUS       DAOPOA010000251         2866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37888_length_2866_cov_3.146923, whole genome shotgun sequence.
ACCESSION   DAOPOA010000251 DAOPOA010000000
VERSION     DAOPOA010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37888_length_2866_cov_3.146923"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKJ17_06100"
     CDS             <1..794
                     /locus_tag="PKJ17_06100"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOD75580.1"
                     /translation="AGQTYDSVVKGIADIGESVLGYTKGRFPLTEVIDLPLGYKNAYA
                     ATRMINAYFKKFQPKEFDQVKVMYLHAHGPGILFTKRPVQNLEELKGMKVRSTGFSAK
                     VAAALGASPVAMPQTESYEALRTGIVEGILNPIEAMKGWKIGEVVKYTIENYGSAYST
                     SFFVVMNKEKWKALPPDIQKTIEGINEEWMEKQGRQWDAIDKEGREFMVQRGNKFIAL
                     SKAEDARWAAKVRPVIDDYVKELKTKGLPGEEALKFCLEYLKANQ"
     gene            913..2247
                     /locus_tag="PKJ17_06105"
     CDS             913..2247
                     /locus_tag="PKJ17_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75581.1"
                     /translation="MAHQMNTYWNPILETMPREKLRELQLRKFKRIVEYAYENSPFYR
                     RLYSAAGMEPGDIKTFEDIAKVPKTDKGMLRKVQDNPPFPYGDILTVPLDEVTEFRQT
                     SGTTGTPVYQADTWQDWEWWSECWSYILYSQGYRSSDRVFLPFGYNIFVAFWAGHYAL
                     EKIGCEVVPGGVLDTEARILKMQELKCTAFMATPTYVLGMADTARKMGIDPTRDLNIQ
                     RITCAGEPGASIPATKKRIEEAWNAKVYDHVGATEIGAWSYECLAQSGGLHVNDAFFL
                     VEIEDVETGEIIDTPGKTGKMVITAFDRVAKPCIRFDSKDVIRWSDRPCECGRTFRMI
                     EGGVVGRVDDITKVKGVLLAPTAIEEVVRSIPEIGNDYQVTVSKRGDIDDILLEIEMM
                     PGMEARQEEVLGRLKDQLRVRVNLGFKITVHPFGVVPRYEVKAKRFKDLRKH"
     gene            2474..2656
                     /locus_tag="PKJ17_06110"
     CDS             2474..2656
                     /locus_tag="PKJ17_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75582.1"
                     /translation="MMDDAKIAEMDRKVEALREMVQDLIDSAGDVEAVKRNAKRILAS
                     VKMLEINICDVAATRV"
     gene            2668..>2866
                     /locus_tag="PKJ17_06115"
     CDS             2668..>2866
                     /locus_tag="PKJ17_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOD75583.1"
                     /translation="MDIIFNGVRENVPEKTAISDLIVKTDEKDGGLIVEVNRRFVFPA
                     DYGKTFLSEGDTVEFINPDFGG"
BASE COUNT          712 a          724 c          900 g          530 t
ORIGIN      
        1 cggccggtca gacctacgac agtgttgtga aagggatcgc cgatattggc gagagcgttc
       61 tcggttacac gaaaggcagg ttccctctta cggaggtcat cgatctgccc ctcgggtaca
      121 agaatgccta tgccgcgaca aggatgatca acgcctactt caaaaagttt cagccgaaag
      181 agttcgatca ggtaaaggtc atgtacctcc atgcccatgg accgggcata ctcttcacga
      241 agaggcccgt ccagaacctc gaggagttga aggggatgaa ggtccgttcc acgggattca
      301 gcgcgaaggt ggccgccgct ctgggggcgt ctcccgtcgc gatgccccag acggagtcct
      361 acgaggctct gcgcaccggg atcgtcgagg gcatcctgaa ccccatagag gcgatgaagg
      421 gctggaagat tggcgaggtg gtcaaataca cgatcgagaa ctacggttcc gcgtacagca
      481 catccttctt cgttgtcatg aacaaggaga agtggaaagc cctgcctcct gacatccaga
      541 agaccataga gggcatcaat gaagagtgga tggaaaagca gggcaggcaa tgggatgcca
      601 tagacaagga gggcagggaa ttcatggttc agcgcggcaa caagttcatc gccctgtcga
      661 aggccgagga tgcgagatgg gcggcgaagg tccgtcccgt catcgacgac tacgtcaaag
      721 agctcaagac gaagggcctc cccggtgaag aggcattgaa gttctgcctg gagtacttga
      781 aggcaaacca gtaatcggca gccccacgca ttgcgggccg ggactacccc ctcctgaatg
      841 ggtcatggca accggctcgc agtgctttac aatgtgatcg gagttgaaga aaatcaggga
      901 ggttgcggcg atatggctca tcagatgaac acatactgga atccgattct cgagacgatg
      961 ccgagagaaa agctgcgcga gctccagctc aggaagttca agaggatagt ggaatacgcg
     1021 tacgaaaact cgcctttcta ccgaaggctt tacagtgccg cgggcatgga gcccggagac
     1081 atcaagacct tcgaggacat cgcgaaggtc ccgaaaacgg acaaggggat gctcaggaag
     1141 gtgcaggaca atcccccctt tccctatggg gacatcctca ccgttcccct ggacgaggtg
     1201 acggagttca ggcagacgag cggtacgacg ggcactcccg tctaccaggc cgatacctgg
     1261 caggactggg aatggtggtc cgaatgctgg tcatacatac tctattctca gggatacagg
     1321 tcgtcggaca gggtctttct gcctttcggc tacaacatat tcgttgcttt ctgggcagga
     1381 cattacgcgc tggagaagat aggttgtgag gtcgtgccgg gcggtgtcct cgacaccgag
     1441 gcccgcatcc tcaagatgca ggagctcaag tgcacggcgt tcatggcgac tccgacgtac
     1501 gtcctgggga tggccgacac ggcgcggaaa atgggcatcg accccaccag ggacctgaac
     1561 atacagagaa taacctgcgc cggcgaacct ggcgccagta tcccggcaac caagaaacgt
     1621 atcgaggagg cctggaacgc gaaggtctac gaccatgtag gagccacgga gatcggggca
     1681 tggagctacg aatgtctcgc gcagtcgggg gggcttcatg ttaacgacgc cttctttctt
     1741 gtcgagatcg aggacgtcga gacgggcgag atcatagaca ccccgggtaa gacaggcaag
     1801 atggtcatca ccgcctttga cagggtcgcg aagccttgca tacgtttcga ttccaaggac
     1861 gtcatccgtt ggtccgaccg cccctgcgag tgcggccgga ccttcaggat gatcgagggc
     1921 ggcgtggtcg gcagggtaga cgacattaca aaggtgaaag gcgtgctcct ggcgccgacc
     1981 gccatcgagg aagtggtgag gagcataccc gagatcggta acgattacca ggtgacggtc
     2041 tcgaagagag gcgacattga cgatatcctc ctcgagatag agatgatgcc gggaatggag
     2101 gccaggcagg aggaggtcct cggccgcctg aaagaccagt tgcgcgtccg cgttaacctg
     2161 ggtttcaaga tcaccgtcca ccccttcggc gtcgtgccgc ggtacgaggt gaaggcgaag
     2221 aggttcaaag acctcaggaa acattaaagg atggtttcag agttcaaggg ttcaagggaa
     2281 gacgaagaag atggtttcaa gttgcgcgtt tcgtgccaga acaagctgcc gacaagaacg
     2341 ttcccggtca ttccggtcct cgccggaacg acagcccgcg agcgggatga agaaaagagg
     2401 ggtttccccc ggttttttgc cttgaacctt gaaccttgaa cttttgaacc attattatat
     2461 ccaggagatg actatgatgg atgatgcgaa gatagctgag atggacagga aggtggaagc
     2521 tctcagggag atggtgcagg accttatcga ttcggccgga gacgtggagg cggtgaagag
     2581 gaacgcgaag cgcattctgg cgagcgtgaa gatgctcgag atcaacatct gcgacgtcgc
     2641 cgccacccga gtctgagcag aaggaacatg gacataatat tcaacggcgt gcgcgagaac
     2701 gtaccggaga agactgccat atcggacctg atcgtaaaga cagatgagaa ggacggagga
     2761 ctgatcgtgg aagtgaacag gaggttcgtc tttcccgccg actacggaaa gactttcctg
     2821 tcggagggcg atacggtaga attcatcaac ccggattttg gaggat
//
LOCUS       DAOPOA010000252         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_37994_length_2860_cov_2.766845, whole genome shotgun sequence.
ACCESSION   DAOPOA010000252 DAOPOA010000000
VERSION     DAOPOA010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37994_length_2860_cov_2.766845"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKJ17_06120"
     CDS             <1..210
                     /locus_tag="PKJ17_06120"
                     /inference="COORDINATES: protein motif:HMM:NF037048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75584.1"
                     /translation="PGDLSGYKLIIHCGACMINRKEMLHRLMVAKHAGVPIVNYGVLI
                     AHVMGILERTLKPFPEVAGAMEDNA"
     gene            277..1689
                     /gene="hydG"
                     /locus_tag="PKJ17_06125"
     CDS             277..1689
                     /gene="hydG"
                     /locus_tag="PKJ17_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOD75585.1"
                     /translation="MDRRHGWERADFLDEKLIADTLEKAKRASVEEARDIVQKGREAK
                     GLTLYETAVLLHTVDGDVRAAVFAAAREVKEKIYGKRLVFFAPLYISNYCTNNCVYCG
                     YRRDNTIARRKLGLDEMEREVIALETMGHKRIALEVGEDPVNCPLDYVIESMGRIYSV
                     KEKMGSIRRINVNIAATTVEEYRRLKAAGIGTYILFQETYHRETYGKMHPAGPKRDYD
                     WHTMAMDRAMEAGIDDVGLGVLFGLYDFKFEVLGLLLHSLHLEERFGVGCHTISVPRM
                     RPAEGVNVQTFPHLVSDDDFKQMVAVIRLAVPYTGMILSTREPQGYRDEVIALGISQI
                     SAGSCTGVGGYHRDIERPAEDTGQFQVEDHRTTDEMLQSVCQAGYLPSFCTACYRQGR
                     TGDRFMALAKTGEIQNICQPNAILTFKEFLLDYASERTKKVGEEALLRHLESVPNARL
                     REEVRRRLERLEAGERDLYF"
     gene            1802..2248
                     /locus_tag="PKJ17_06130"
     CDS             1802..2248
                     /locus_tag="PKJ17_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOD75586.1"
                     /translation="MKTAKDMLKDKKKDIWSIGPNKTVFDALKIMGEKEVGALMVIDD
                     KGKVHGIISERDYARKIILKGKTSAQTKVSEIMTPADKMFTVKPETSVEDCMVLITAK
                     RIRHVPVFDNDKFVGLISIGDVVKSIISEKDMLIEHLSNYIAGKYV"
     gene            complement(2252..>2860)
                     /locus_tag="PKJ17_06135"
     CDS             complement(2252..>2860)
                     /locus_tag="PKJ17_06135"
                     /inference="COORDINATES: protein motif:HMM:NF018651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75587.1"
                     /translation="DRYLLRFETSDPALFARLHPGSSLDDRLRCIEDLKDLGVQTGSG
                     FMIGLPGETVGVLADNILLCRRLDLDMIGIGPFIPHPDTPLCGTKNAYHDDPDMFFRA
                     LATLRIFNPDAHIPATTAFDAVFPGEGRNLALARGANVFMPNITPVRYKKEYLLYPGK
                     PSVDEEPEECRTAAIARIESLGRRVGRGPGHSFKKREQPGQR"
BASE COUNT          659 a          776 c          879 g          546 t
ORIGIN      
        1 cccggggacc tgtcggggta caagctgatc atacattgcg gcgcctgcat gataaatcgc
       61 aaggaaatgc tccaccgcct catggtggcg aagcacgccg gggtgcccat cgtcaactac
      121 ggtgtgctca tcgcccatgt catgggcatc ctggagagga cgctgaagcc cttccctgag
      181 gtcgccggag ccatggaaga caacgcctga aggactcccg accttccttt acatttcgat
      241 accggggcaa tatacttata cgacggaggc cacagcatgg atcgacggca tggatgggag
      301 cgggccgatt tcctcgatga aaagctcata gcagataccc ttgaaaaggc gaaacgcgcc
      361 tccgtcgaag aggcgcggga cattgtgcag aaaggccgcg aggcaaaggg tctgaccctc
      421 tacgagacgg ccgtgctcct tcacaccgtc gacggagacg tccgggcggc ggtcttcgcg
      481 gccgcgcggg aggtgaagga gaagatctac ggcaagcgcc ttgtcttctt cgcgccgctc
      541 tacatcagca attactgcac gaacaactgt gtttattgcg gatacaggcg ggacaacacc
      601 atagcaagac ggaagctcgg tcttgacgaa atggagcgcg aggtcatcgc cctcgagacg
      661 atgggacaca agcgaatagc ccttgaggtc ggagaggacc ccgtgaactg tccccttgac
      721 tacgtcatcg agtccatggg gcggatatac tccgtcaagg agaagatggg cagcatacgc
      781 cgcatcaacg tcaatatcgc cgccaccacc gtggaggagt accgcaggct caaggcggcc
      841 ggtataggaa cctatatcct tttccaggag acctatcacc gcgaaacata tggcaagatg
      901 catccggcgg ggcccaagag ggactacgac tggcacacca tggcaatgga ccgggccatg
      961 gaggcaggca tcgacgacgt gggactgggt gtcctcttcg gcctgtatga tttcaagttc
     1021 gaggtccttg gcctgctcct tcattcgctt catcttgaag agcgtttcgg ggtcggctgc
     1081 cacaccatat ccgtgccgag gatgcgacct gcagaggggg tcaatgtgca gacatttccc
     1141 caccttgtca gtgacgatga tttcaagcag atggtggcgg ttatcagact tgccgtcccg
     1201 tacacgggta tgatcctttc cacgagggag ccccagggct atcgcgacga ggtcatcgcg
     1261 ctcggcatct cgcagatcag cgcgggttcg tgcacggggg tcggaggata tcaccgggac
     1321 atagaaaggc ctgcggagga cacgggccag ttccaggtgg aggaccatcg gaccaccgac
     1381 gagatgctcc aaagcgtttg ccaggcagga taccttccga gcttctgcac cgcctgctac
     1441 cggcagggaa ggacgggcga caggttcatg gccctcgcga aaacggggga gatacagaac
     1501 atctgccagc ccaacgcgat actgacgttc aaggaatttc tcctggacta tgcctcggaa
     1561 agaacgaaga aagtgggcga agaggcgctc ctcagacacc ttgaatcggt acccaacgcc
     1621 aggctgcgcg aagaggtcag acggcggctt gaaagactcg aggcaggaga acgagatctc
     1681 tatttttgag aagggtgcca aaatgccctt gcctgccccg tgctttgatt atatacttta
     1741 gcaacacggg cttggatctg cacaggggtc atgaaaaaaa agagaagggg gcttaagagc
     1801 tatgaagacc gcaaaggaca tgctcaaaga caagaagaag gacatttggt ccatcggacc
     1861 gaacaagacc gtttttgacg cgctgaagat catgggtgag aaggaggtcg gcgcgctgat
     1921 ggtcattgac gacaaaggaa aggtgcacgg gataatatcg gagagggact acgcgcgcaa
     1981 gatcatcctc aaggggaaga cgtcggccca gaccaaggtg tcggagatca tgaccccggc
     2041 ggacaaaatg ttcacggtga agccggagac atccgtcgag gactgcatgg tcctcatcac
     2101 cgcgaaacgc ataaggcacg tccccgtgtt tgacaacgat aagttcgtcg gccttatctc
     2161 catcggtgac gtggtcaaat cgatcatatc cgagaaggac atgctcatcg agcatttgag
     2221 caattatatc gccggcaaat acgtttaaag atcacctctg tcccggttgc tcccttttct
     2281 tgaaggagtg gccgggacct cgtcccactc gtctccccag ggactcgatc cgtgctatcg
     2341 cggcggtcct gcattcctcg ggctcttcat ccacagaagg ctttcccgga tagagaaggt
     2401 attccttctt gtagcggacg ggagtgatat tgggcatgaa gacgttcgcg ccccttgcga
     2461 gggcgaggtt ccgtccctcg ccgggaaaga cggcgtcgaa ggctgttgtt gccggtatgt
     2521 gggcgtcggg gttgaatatg cgcagcgtcg ccagggccct gaagaacata tcggggtcat
     2581 cgtgataggc gttcttcgtt ccgcacagag gggtgtccgg gtgggggatg aaggggccga
     2641 tcccgatcat gtcgaggtca aggcggcggc acaggagtat gttgtccgcc agtactccca
     2701 cggtctcacc cgggagccct atcatgaatc cgcttcccgt ctggaccccg aggtccttga
     2761 ggtcctcgat gcagcgcagc ctgtcgtcga ggctcgatcc cggatggagg cgcgcgaaaa
     2821 gtgccgggtc cgatgtctcg aaacggagaa gatatcggtc
//
LOCUS       DAOPOA010000253         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_38005_length_2859_cov_4.224322, whole genome shotgun sequence.
ACCESSION   DAOPOA010000253 DAOPOA010000000
VERSION     DAOPOA010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38005_length_2859_cov_4.224322"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="PKJ17_06140"
     CDS             <1..1051
                     /locus_tag="PKJ17_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD75588.1"
                     /translation="EFVHEKDRERMLGYHARRRVEPESVPRNYEFSLVDRQGTVRDVI
                     ITISLIPGTKKSVASFADISDRRRSEERLRESEDRYRTIFETTGTATIIVEEDTTVTL
                     VNTEFEKLSGAPKDYWEGKRKWTEFVHEKDRDRMLGFHRSRRINPESAPRNYEFSLVN
                     MQGDVREVIITISMIPGTKRSVASFVDITKRKQAEEALKKNERDLKDKTHELEELNAA
                     LRVLLKRREEDKLELENNVVSNLKKLVIPYIEKLKKGRIDRNDLVSLNVIESNLKDIA
                     SPFASKLSSEHLSLTPKELQVADLIKEGKTTKEIAEFMSLSPATVEIHRYHIREKLGL
                     SRKKTNLRTYLSSLK"
     gene            1270..2637
                     /locus_tag="PKJ17_06145"
     CDS             1270..2637
                     /locus_tag="PKJ17_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOD75589.1"
                     /translation="MSKYGGKSATAEQVAALVRDGMWVDIGGVQIARAFDRALAERAG
                     ELKNVKVRMIGHLTPLEIMKKDPGAQSFWTGNWHILGHTRAYIAENRGSHIPYNFGET
                     ARYYRDSLDVDIAVQTVTPMDKHGYFNFGPLCNFKKAVFEKAKIKVVEVVEDMPWCYG
                     GYDECIHLSDVDHVIENKTDKLITIPSPPATDTDKKIAGRIAEMIPKDGPCLQIGIGG
                     LPDTVLRLLADSGARDIGIHTEMVTEAIMELHEAGLITGKKKTFLPEKIVWAFAIGSR
                     KLYDWIDHNPALAMYPVDFTNDPCVIAMNKDLISINACLGVDLQGQVSSESIGPAEYS
                     GTGGQLAFVRGAYYRATKGQPPLGYPGNKSILAFYSTYTDSKGTLQSRIKPTIPPGEI
                     VTVPRVDVSYLVTEYGVAYLKGLSIPERVLAIVKLAHPDFRDGLIEEAKKMHWLNRQW
                     FLGSI"
     gene            2643..>2859
                     /locus_tag="PKJ17_06150"
     CDS             2643..>2859
                     /locus_tag="PKJ17_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75590.1"
                     /translation="MRLKGFEGEKVGKAAACDGPQPVQPSYPLTFLPSQSAGDAPFDR
                     MTDKDRRNAGMAIKKVCVLGAGLMGSGI"
BASE COUNT          673 a          869 c          842 g          475 t
ORIGIN      
        1 cgagttcgtc cacgaaaagg accgggagcg gatgctcggg tatcacgccc gcagaagggt
       61 cgagcccgag tccgtgccgc ggaactacga gttcagcctc gtcgaccggc agggcacggt
      121 ccgggacgtg atcatcacga tttccctcat cccgggcacg aagaagagcg tcgcctcctt
      181 cgccgacatc tcggaccgca gaaggtccga ggagcgtctc cgggagtcgg aagaccgcta
      241 ccgcacgatc ttcgagacga cggggacggc cacgatcatc gtcgaggagg acacgacggt
      301 caccctcgtc aacacggagt ttgaaaagct ctcgggggcc cccaaggact actgggaggg
      361 aaagcgcaag tggacggagt tcgtccacga aaaggatcgt gaccggatgc tcggatttca
      421 ccgcagccgc cgcatcaacc ccgagtcggc gccgcggaac tacgagttca gcctcgtcaa
      481 tatgcagggg gacgtccggg aagtaatcat cacgatctcg atgatcccgg gaacgaaacg
      541 gagcgtcgcc tccttcgtgg acatcacgaa gcgcaagcag gccgaggaag cgctgaagaa
      601 aaacgagcgg gacctcaagg acaagacgca cgaacttgag gagctcaacg cggcgctgcg
      661 cgtcctgctg aagcgcaggg aggaggacaa gctcgagctg gagaacaacg tcgtctccaa
      721 cctgaagaaa ctggtgatac cctacatcga aaagctcaag aagggccgta tcgatagaaa
      781 cgacctcgtg agcctcaacg tcatcgagtc caacctgaag gacatcgcct ctcccttcgc
      841 gagcaagctc tcgtcggagc acctgagcct cactcccaag gaactccagg tggccgacct
      901 catcaaggaa ggcaagacaa ccaaggaaat cgccgagttc atgagcctgt cgccggccac
      961 cgtcgagatc caccggtatc acatccggga gaaactcggt ctgtcccgaa agaagaccaa
     1021 cctgaggacc tacctctcct ccctgaagta acctttagaa aaactaaagg ttatgtaccc
     1081 attctttaaa tattgcctct ttcccctttc cgcatttaag aagagtgcga atccattttc
     1141 ttcgtgcagc ctgtaacctg ccctccccgt gccggagcgg cgggtgaacc cgatagacgt
     1201 tcgatccgcc gacgagacaa tcaacctgcc ccacagcggg caggcaaaat cctggaggga
     1261 ggacgagtga tgtcgaagta cggagggaaa tcggcaacgg cggaacaggt ggccgccttg
     1321 gtcagggacg gcatgtgggt ggatatcggg ggcgttcaga tagcccgggc attcgatcgc
     1381 gccctggccg agagggcggg agagctgaaa aacgtcaagg tgcggatgat cggccacctc
     1441 acccccctgg agatcatgaa gaaagatcca ggggctcaga gcttctggac gggcaactgg
     1501 cacatcctcg gccataccag ggcgtatatt gccgagaacc ggggatccca cattccgtat
     1561 aatttcgggg agacggcgcg atattatcgg gacagcctcg atgtcgatat cgccgttcag
     1621 accgtcaccc cgatggacaa gcacgggtac ttcaatttcg gccccctgtg caacttcaag
     1681 aaggccgtct tcgagaaggc gaagatcaag gtcgtagaag tcgtcgaaga catgccctgg
     1741 tgctatgggg gttacgatga atgcatccac ctctccgacg tggaccacgt catcgaaaac
     1801 aaaacggaca agctgattac catcccctcg ccgcccgcga cggacacgga caagaagatc
     1861 gccggacgca tcgccgagat gatccccaag gacggcccct gcctccagat cggcatcggg
     1921 gggcttcccg atacggtcct gcggttgctg gccgacagcg gcgcccgcga catcggcatc
     1981 catacggaga tggtcaccga ggctatcatg gaactccacg aggcgggact gatcaccggg
     2041 aagaagaaaa ccttcctgcc ggaaaagatc gtctgggcct tcgccatcgg cagccgcaag
     2101 ctctacgact ggatcgacca caacccggcg ctggccatgt atcccgtcga tttcaccaac
     2161 gacccctgcg tcatcgcgat gaacaaggac ctgatctcca tcaacgcctg cctcggcgtc
     2221 gatctccagg gtcaggtgag ctccgagtcg atcggccccg ccgagtactc gggcacgggc
     2281 ggccagctgg ccttcgtccg cggcgcctac taccgcgcca ccaagggcca gccgcctctc
     2341 ggctaccccg gcaacaagtc gatcctcgcc ttctattcga cctacacgga cagcaaggga
     2401 acgctgcaat cgaggatcaa gcccaccatc ccgcccggcg agatcgtgac ggtaccgcgg
     2461 gtcgacgtct cgtacctcgt cacggaatac ggcgtcgcgt acctcaaggg cctctccatc
     2521 ccggagaggg tcctggccat cgtcaagctg gcgcaccccg atttccgaga cgggctcatc
     2581 gaggaagcca aaaagatgca ctggctcaac aggcagtggt ttttggggag catctgaacg
     2641 gaatgaggtt gaaagggttt gaaggtgaga aggttggaaa agctgcggcc tgcgatggac
     2701 cacagcccgt ccagccttct taccctctca ccttcttacc ttctcaatct gctggcgacg
     2761 ccccatttga caggatgacg gacaaagaca ggaggaacgc aggtatggca atcaagaagg
     2821 tttgcgtact cggagcgggg ctgatgggca gcgggatcg
//
LOCUS       DAOPOA010000254         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_38073_length_2855_cov_3.266429, whole genome shotgun sequence.
ACCESSION   DAOPOA010000254 DAOPOA010000000
VERSION     DAOPOA010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38073_length_2855_cov_3.266429"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PKJ17_06155"
     CDS             complement(<1..934)
                     /locus_tag="PKJ17_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD75591.1"
                     /translation="MYTEYWGLKKPPFDNVPDPSMYVDSHLSVENTVAETLFAIEEGN
                     ECLTVIVGDVGLGKTLSLRLILDSLDQSKYKIAFVTNPDMSFVQILREIIGQLTGKEC
                     EIRGKTELLEVFNKLLFETADEGKKVLVFIDEANAISPTNLESLRLLTNMQDDTRNLF
                     TIVLAGQMELAKRLEHPKRANLFQRIGTYNRIEKMDSEELVRNYVDMRLKMAGAARQI
                     FTDDGVRRLYQYSENGVPRLINKIAKLCLKAGETNNFDVITGDIVQQIGQRFLKMTGP
                     AVQKRGVKERPVRKAKAAERAVVETPPAAMPQPPE"
     gene            complement(1010..1579)
                     /locus_tag="PKJ17_06160"
     CDS             complement(1010..1579)
                     /locus_tag="PKJ17_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD75592.1"
                     /translation="MEKICIVKRRKTTAQATQDPVVEIHTAPPVTSPVRDQEVAEIEQ
                     IPEVSVPAPAIGSPESFNIQDGDHLIFKEVAGEEEQIFSIQLSPQQSEIVQSMNCIKD
                     LLSGKHHGVKMSMEQTADGSTAFNFHFKPVYTTRMLNPNDVSTMLQISKSMLYRLVKE
                     KEIKSYKIGKLRRFLLEDVVDYLSSSLQM"
     gene            2113..>2855
                     /locus_tag="PKJ17_06165"
     CDS             2113..>2855
                     /locus_tag="PKJ17_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOD75593.1"
                     /translation="MEATTSTAKDKESGIKEIVRSLGLVFGDIGTSPIYTITIVFLLM
                     KPTEANVIGVLSLILWTMTVLVSIEYAWLAMSLGEKGEGGAFVLRGILVRLLKKTRSM
                     VFVTVITFIAVSLLAGDGVITPAISILSAVEGILLIPGFSGTRQTTLIVIAGIIAIIL
                     FAFQKKGTDKVAGAFGPLMVMWFLALSLSGVLSIIHNPSVLKAVNPWYAARFLWHEGI
                     TGFFVLSEVILCEDEDAGNPLVPEESRRVP"
BASE COUNT          602 a          813 c          731 g          709 t
ORIGIN      
        1 tctccggcgg ctgcggcatg gctgccggcg gtgtctccac gacggcccgc tcggctgcct
       61 ttgccttcct aacaggccgt tccttcacgc cccgcttttg aaccgcgggt cccgtcatct
      121 tcaggaaacg ctgaccgatc tgctgaacaa tgtctccggt gatgacgtca aagttattcg
      181 tttcccccgc cttcaggcac agcttcgcta tcttgttgat gagacgggga acaccgttct
      241 cggagtactg gtagagccgc cggacgccgt catccgtgaa gatctgcctc gcggcacccg
      301 ccatcttcag gcgcatgtcg acgtaatttc tgacaagctc ctcgctgtcc atcttttcga
      361 tcctgttgta cgtaccgata cgctggaaca ggttcgccct cttggggtgc tcgagcctct
      421 tggcaagctc catctggccg gcgaggacta tggtgaaaag gtttctcgta tcgtcctgca
      481 tgttcgtgag aagccgcagg ctctcaagat ttgtcggcga tatggcattg gcctcgtcga
      541 taaagacaag gactttcttc ccctcgtcgg ctgtttcgaa gagaagcttg ttgaacacct
      601 cgagaagttc cgttttcccg cggatctcac attccttgcc cgttaactgg cctatgatct
      661 cgcggagtat ctggacgaag gacatgtccg ggttcgtcac aaaggctatc ttgtacttgc
      721 tctggtcaag cgagtcgagg atgaggcgca acgacaacgt cttgccaaga cccacatcgc
      781 ccacgatcac cgtgaggcat tcattcccct cctcgatggc gaagagcgtc tccgcgacag
      841 tgttttccac tgagagatga gaatcgacat acatagacgg gtccggcaca ttgtcgaaag
      901 gcggcttttt caggccccag tactcagtgt acatgtgttg tgtcctcctt ctgtcaggca
      961 acaggaacta cctgcccgat tcccaccgca cagcgtgatc gctgtttcgt tacatctgca
     1021 ggctgctgct caaataatcc acaacatctt caaggagaaa ccgcctgagc ttccctatct
     1081 tgtaactctt gatctccttc tccttcacca ggcggtaaag catgctcttg cttatctgga
     1141 gcatcgtgga aacatcgttg ggattcagca tacgagtggt atagacaggt ttgaaatgga
     1201 aattgaacgc cgtgctcccg tcggctgtct gttccatgga catcttgaca ccgtgatgct
     1261 tgccgcttaa gaggtctttt atgcagttca tggactggac gatctcggat tgctggggac
     1321 tcaactgaat cgaaaagatc tgctcctctt cccccgccac ttccttgaat atcaggtggt
     1381 ccccatcctg gatgttgaag ctctcaggcg aacctatggc aggagcggga actgatactt
     1441 cggggatctg ctctatctcg gcaacttcct ggtcgcggac cgggcttgtg acgggtggag
     1501 ccgtatgaat ctcaacaacg ggatcctggg tcgcctgcgc tgtcgttttt ctccgtttga
     1561 ctatgcaaat cttttccatg actccccttc gagatatggt atttattact tcctcattat
     1621 cgcctgcttt gaaaaacgct ttacttcact attaaaaccg atatttatat gcatttcata
     1681 tcaatttact tccagaacag aactgactac attagaactt attccaaaaa aggtgtcaat
     1741 caaatttcct aagccccaat cgtcccttga gaccggggtc tcgtatcccg cagtcgacat
     1801 ccgggcctcg gaaagttctc tgacaggaaa tggccgatga ggtgaactgg caagcttcgg
     1861 gttagaaatg acgtgaagag atgataaaag accggaaaaa cgaattgatg aacatttcgt
     1921 cgtcttcatg gtcccgccgc cttcgaggaa ggtcatacaa tttattacct gcaggctttg
     1981 caacaaaata tagaagatgt ggccctcact gtcaacgcat ttttcgggcc cggcggacat
     2041 ggcgcgcatt gcttttctcc gggaatgggt ctagaatgga aactgccaga tatcagggaa
     2101 ttcgcggacc atatggaagc cacaacctcg acggcgaagg acaaggagtc cggcatcaaa
     2161 gagatcgtgc gatccctggg gctcgtcttc ggggacatcg gcacgagccc catctacacc
     2221 atcaccattg tatttctcct catgaaaccc acggaggcga acgtcatcgg ggtcctttcg
     2281 ctgatcctct ggacaatgac ggtcctcgtg agcatcgaat acgcgtggct ggccatgagc
     2341 ctcggcgaga agggcgaagg gggcgcgttc gtgctgaggg ggatactggt gcgcctcctg
     2401 aagaagaccc gctccatggt cttcgtcacc gtcatcactt tcatcgccgt ttccctcctc
     2461 gccggggacg gcgtgataac tcccgccatc agcatcctga gcgccgtcga ggggatactc
     2521 ctcataccgg gtttcagcgg aacacggcag acgacgctca tagtcatcgc cgggatcatt
     2581 gccatcatac ttttcgcctt ccagaaaaag ggtaccgaca aggtggccgg ggccttcggt
     2641 cctctcatgg tgatgtggtt cctggcgctt tcactgtccg gtgtcctgtc catcatacac
     2701 aacccctcgg tcctcaaggc cgtcaacccc tggtacgcgg cgagattcct ctggcacgag
     2761 gggattaccg gcttcttcgt cctctcagag gtcatcctct gcgaggacga agatgccggt
     2821 aatcccctcg tgccagagga atctcgccgc gtacc
//
LOCUS       DAOPOA010000255         2847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_38222_length_2847_cov_2.931948, whole genome shotgun sequence.
ACCESSION   DAOPOA010000255 DAOPOA010000000
VERSION     DAOPOA010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38222_length_2847_cov_2.931948"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..1449)
                     /locus_tag="PKJ17_06170"
     CDS             complement(334..1449)
                     /locus_tag="PKJ17_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOD75594.1"
                     /translation="MDIINEYLKYMVEKDASDIYFTVGVPPAFRIEGVVSLYGDKALT
                     AEDTKSIAYAIMNERQQAKFEEEMEMNLAIFQPEYGRFRVNIFRQKSFIGIVIRQIKL
                     TIKTIDDWGLPAIMKDICMSKRGLVLVVGATGSGKSTTLAALIDYRNSNQPGHIITIE
                     DPVEFVHQHKMSVVTQREVGFDTLSFYNALKNTLRQAPDVILIGEIRDAETMEDAITF
                     AETGHLCLATLHANNANQALERILNFFPIERHLQIYMQLSLNIRSIVSQRLIPTVEGK
                     RAAAIEILLDSPRVKDLILKGEITLLKETMAESYFEGMQTFDQHIFDMYQSGLLDLNN
                     AIAYADSPNDVRLKIKMAEMKPDDAAEKKDSGLKLKL"
     gene            complement(1453..2487)
                     /locus_tag="PKJ17_06175"
     CDS             complement(1453..2487)
                     /locus_tag="PKJ17_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOD75595.1"
                     /translation="MDISELLRFALESGGSDLHISAGEPPVIRIHGEMTKVDLPPLDK
                     EDVHNMIYDILSDFQRKVFEEHLELDFSFGLGDLARFRVNVFKQQRGESAVFRTIPSK
                     IPTFDELNLPRVFKDIAGLEKGLVLVTGPTGSGKSTTLAAIVDFINDTVKEHILTIED
                     PIEFLHNSKKCLVNQRELGPHTKSFANALRSALREDPDVILVGEMRDLETIQLALTAA
                     ETGHLVFATLHTSSAPDTVDRVIDVFPAGQQNQVRSMLSGSLQAVISQALFKRRDGKG
                     RVAGFEVMIATPAVRNLIRENKIAQIPSMIQTSKGIGMQTMEAACNDLIAKNLVTRDE
                     VSFYLSSSMR"
     gene            complement(2497..>2847)
                     /gene="scpB"
                     /locus_tag="PKJ17_06180"
     CDS             complement(2497..>2847)
                     /gene="scpB"
                     /locus_tag="PKJ17_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOD75596.1"
                     /translation="EVAGGFQMRTKVLYRDWVKRFVREKDVGLSRPMLETLSIIAYKQ
                     PVTKKEIDVVRGVDSARAVKHLLERRLIEIAGRNGEAGKRMVFRTTQRFLEVYGLKSL
                     EDLPTYKEIESLEG"
BASE COUNT          529 a          800 c          836 g          682 t
ORIGIN      
        1 ccctcttccc ctcgacggga aaagcgatcg ccgtctcttc agcggaaaca tcagaaactg
       61 gaaccagaac agacatcaaa gataccgcaa gcagcaacaa tgcagcgcca atacccgttt
      121 tcctcatagt catcatcctc ggtttctctg aaggcggctt gaatggcttg agtcgcttaa
      181 cagactgttg acaaactcct cttttcgtca tcccggactt gatccgggat ccaaaggttt
      241 ttctaaccca ttgtgttttc tggattccgg ccttcgccgg aatgacggtg ataataaacg
      301 gttctaacgg ctcaagcgat tcaagagccg cctctagagc ttcagcttca gtcccgaatc
      361 cttcttctcc gccgcgtcgt cgggcttcat ctcggccatc tttatcttga gccgcacgtc
      421 gttggggctg tccgcgtagg cgatggcgtt gttgaggtcc agaaggcccg actggtacat
      481 gtcgaagatg tgctgatcga aggtctgcat cccttcgaaa taggactccg ccatggtctc
      541 tttcaggagg gtgatctcgc ccttgaggat gaggtccttg accctcgggc tgtcgagaag
      601 gatctcgatc gctgccgcgc gcttgccctc cactgtgggg atgagcctct gggatacgat
      661 ggaacggatg ttgagggaaa gctgcatgta gatctggagg tggcgttcga tgggaaagaa
      721 attgagtatc ctttcgaggg cctggttcgc gttgttcgcg tggagagtgg cgaggcaaag
      781 gtggcccgtc tccgcaaagg ttatggcatc ctccatcgtc tccgcatccc tgatctcacc
      841 gatgaggatg acgtcgggcg cctgccgcag ggtgttcttg agggcgttgt agaaactcag
      901 ggtgtcgaag ccgacctccc tctgcgtgac gacgctcatt ttgtgctggt ggacgaactc
      961 caccgggtcc tcgatggtga tgatgtggcc gggctgattg gaattgcggt agtcgatgag
     1021 agcggccagg gtggtcgact tgccgcttcc cgtggcgccg acgacgagga cgaggccgcg
     1081 cttggacatg catatgtcct tcatgattgc gggcagcccc caatcgtcga tggtcttgat
     1141 cgtgagcttg atctggcgta tgacgatgcc gataaagctc ttctgccgga agatgttcac
     1201 ccggaacctg ccgtattcgg gctggaagat ggccaggttc atctccatct cctcctcgaa
     1261 cttcgcctgc tgccgctcat tcatgatggc gtaagcgatg ctcttcgtgt cctccgcggt
     1321 gagagccttg tcgccgtaaa gggatacgac gccttcgatg cggaatgccg ggggcacccc
     1381 cacggtgaag tagatgtccg acgcgtcctt ttcgaccatg tacttcaggt actcattgat
     1441 gatgtccatg gatcacctca ttgagctgct caggtagaag gaaacctcgt cccttgtcac
     1501 gaggttcttg gcgatgaggt cgttgcaggc cgcttccatc gtctgcatgc caatcccctt
     1561 gctcgtctgg atcatggagg gtatctgggc gatcttgttc tccctgatga ggttcctcac
     1621 ggccggagtg gcgatcatga cctcgaagcc ggcgaccctc cccttgccgt cacgcctctt
     1681 gaaaagggcc tgggagatga cggcctgcag ggaaccggag agcatggatc gcacctggtt
     1741 ctgctggccc gcggggaaga cgtcgatgac cctgtcgacc gtgtcgggag ccgaactggt
     1801 gtggagggtg gcgaatacga ggtgtcccgt ttcagccgcc gtcagggcaa gctggatggt
     1861 ctcgaggtcc ctcatttcgc cgacgagtat gacgtcgggg tcctcacgca gggcgctcct
     1921 cagggcgttg gcaaagcttt tggtatgcgg tccaagttca cgctggttga caaggcattt
     1981 cttggagttg tggaggaact cgatggggtc ctcaatggtg aggatatgtt ccttcacggt
     2041 atcgttgatg aagtccacta tcgcggcaag cgtcgtcgac ttgccgctgc cggtgggccc
     2101 cgtgacgagg acgagaccct tctccagacc cgctatgtcc ttgaatacgc ggggcaggtt
     2161 gagttcatcg aaggtgggta tcttgctcgg aatggtcctg aagacagccg attcgccgcg
     2221 ttgctgcttg aagacgttga ccctgaagcg ggccaggtcg ccaaggccga aggagaagtc
     2281 gagctcaagg tgctcctcga agaccttccg ctggaagtcg ctcaatatgt cgtagatcat
     2341 attgtgcaca tcttccttgt cgaggggcgg caggtcgacc ttcgtcatct cgccgtgtat
     2401 cctgatcacg gggggttccc cggcgctgat atgcaggtcc gatcctccgc tttcgagggc
     2461 gaaacgcagc agttcagata tgtccatgat gtcctcctat ccttcgagcg attctatctc
     2521 cttatatgtc ggcagatctt caaggctctt taacccgtag acctcgagaa atctctgtgt
     2581 cgtcctgaac accatgcgct ttcccgcctc accgttccgc ccggcgatct cgatgagcct
     2641 tctctccagg aggtgcttga cggcccgagc cgaatcgaca ccgcgcacga catcgatctc
     2701 cttcttcgtg acgggttgct tgtaggctat gatcgatagg gtctcgagca tcggccggga
     2761 gagtcccacg tccttttccc ggacgaagcg ctttacccag tcacggtaaa ggaccttcgt
     2821 cctcatctga aaaccgccgg ccacctc
//
LOCUS       DAOPOA010000256         2840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_38354_length_2840_cov_2.630880, whole genome shotgun sequence.
ACCESSION   DAOPOA010000256 DAOPOA010000000
VERSION     DAOPOA010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38354_length_2840_cov_2.630880"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKJ17_06185"
     CDS             <1..187
                     /locus_tag="PKJ17_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75597.1"
                     /translation="CMVTCKMDDDEAKLLLNVLERYHSHLEVEIVRTNRREFRDALKE
                     REKQLSGLMEKVKALVK"
     gene            complement(223..1008)
                     /locus_tag="PKJ17_06190"
     CDS             complement(223..1008)
                     /locus_tag="PKJ17_06190"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD75598.1"
                     /translation="MPDYSKIDIPEVLSYVFYPRDESGPCPRYAFDHFIPVAEAVALH
                     CRFYEQDKAWPWILYFHGNGEVVSDYDEIALFYFKHNLNLAVVDYRGYGKSNGTPTVA
                     DMSRDSRKVYESVKTALTQRGLRDDLWVMGRSLGSVSALAIAFDKSTGINGLIIESGF
                     PSITSLIVRHGIATPGMDLDAITRECLDMVRSITVPVLIIHGEYDTLVPPDEAETIYE
                     TIGSARKDLLMIPGATHNDIMFVGLRQYMEAIRKFVDSTSPNR"
     gene            complement(1075..1704)
                     /locus_tag="PKJ17_06195"
     CDS             complement(1075..1704)
                     /locus_tag="PKJ17_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOD75599.1"
                     /translation="MIPFVTAAVVLGLSSGFSPGPLLTLVIAQTVTHGVKEGIKVALA
                     PLITDAVIITVAVSVLSRFAHIHTVLGWISICGGLFVIYLAWETFRAPEPRVDLAPKQ
                     ARSLGRGIAVNALNPHPYIFWIAVGAPMMVRAREDSLVSAVAFVLCFFVSLVGAKVLV
                     AVLVGRTKKVLTGRIYRWIMRFLGLALFLFALFLVRDGLVLLTVIAKFP"
     gene            complement(1701..2006)
                     /locus_tag="PKJ17_06200"
     CDS             complement(1701..2006)
                     /locus_tag="PKJ17_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75600.1"
                     /translation="MMTYIGMAFAGLSCLVCVANIVNYTLRMVKKKRGETTHRRTSTI
                     HVLSIVFSIMAYVFAGDTLGAWVFIPAVLDPATLFILAAPILLIRVKRRADREQNPR"
     gene            2327..2539
                     /gene="thiS"
                     /locus_tag="PKJ17_06205"
     CDS             2327..2539
                     /gene="thiS"
                     /locus_tag="PKJ17_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOD75601.1"
                     /translation="MPPQIEIILNGLKEKVLAGTTIADLIKMSGEEDKHLVVERNNRF
                     IHPQTYHATPLSEGDRIEFINPDFGG"
     gene            complement(2547..>2840)
                     /locus_tag="PKJ17_06210"
     CDS             complement(2547..>2840)
                     /locus_tag="PKJ17_06210"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOD75602.1"
                     /translation="SEEVDRKTTVHYILAEMCGNRFFEALVTCVMRLTKQVVEAVKPD
                     TEKLHPAGLHDAAVEAVIAGDPETAAREMEKHAIAFGKNLMEMEEEYHKTKNV"
BASE COUNT          608 a          780 c          884 g          568 t
ORIGIN      
        1 atgtatggtt acgtgcaaga tggacgacga cgaggccaaa ctcttactca acgtgcttga
       61 aaggtaccat tcccacctgg aagtggagat agtaaggacc aacaggcggg agttccgtga
      121 tgccctcaag gagagggaaa aacagctatc ggggctcatg gaaaaggtga aggccctcgt
      181 aaagtagggg ggcctgtccg cgaagccgaa gaccgactga ggctatcggt tggggctcgt
      241 cgaatcgacg aacttcctga tggcctccat gtactgccgc aagcccacga acattatatc
      301 gttgtgcgtc gcccccggta tcatcaggag gtccttccgc gctgaaccga tggtctcgta
      361 gatggtctcg gcctcgtcgg gcggcaccag ggtgtcgtat tccccgtgaa tgatgaggac
      421 ggggaccgtg atcgatcgca ccatatcgag gcactcccgg gtaatggcgt cgaggtccat
      481 gcccggtgtg gctatgccgt ggcggacgat gaggctcgtg atgctgggga aaccgctttc
      541 gatgatgagc ccattgatgc cggtactctt gtcgaaggcg atcgccagag cggaaacgct
      601 gccgagagac ctgcccatga cccacaggtc gtcccgtaaa cccctttgtg tgagggccgt
      661 cttgaccgat tcgtagacct ttctagaatc acgggacatg tccgcgacgg tgggcgtgcc
      721 gttggacttc ccgtaacccc ggtaatcgac caccgccagg ttgaggttgt gcttgaagta
      781 gaagagggcg atctcgtcgt agtcgctcac gacctcgccg ttcccgtgga aatagaggat
      841 ccagggccag gccttgtcct gctcgtagaa acggcaatgg agagccaccg cttccgccac
      901 ggggatgaag tggtcgaagg cgtatctcgg acaggggccg gactcgtcac gcgggtagaa
      961 cacgtaggac aggacctccg gtatgtctat cttgctgtaa tcgggcatga cgttcgctcc
     1021 tctcgataag gattttgcag acttggatat tctatccgtc ccgatgccgc gctttcaagg
     1081 aaatttggcg atcactgtga ggagaacaag gccgtccctg acgagaaaga gggcaaacag
     1141 gaagagggcg aggccgagaa accgcatgat ccaccggtag atccttcccg ttagaacttt
     1201 tttcgtcctg cccacgagga cggcgacgag gaccttcgcg ccgacgagag aaacgaagaa
     1261 gcacaggacg aacgcgaccg ccgagaccag actatcttcg cgggcccgga ccatcatcgg
     1321 ggcgccgacg gcgatccaga agatgtacgg atggggattc agggcgttga cggctatccc
     1381 ccttccgaga gaccgggcct gttttggggc caggtccacc cgcggctcgg gtgcccggaa
     1441 cgtctcccag gcaaggtaga tcacgaagag ccccccgcag atggagatcc atcccagcac
     1501 ggtgtgaatg tgggcgaagc gggacaggac agagacggca acggtgatga tgaccgcgtc
     1561 ggtgataagg ggcgcaaggg caactttgat cccttccttc acgccgtgcg tcaccgtttg
     1621 cgctatgaca agggtcagga gcggccccgg agagaaaccc gacgagaggc cgagaaccac
     1681 ggcggcggtg acgaaaggta tcatcgaggg ttctgttccc ggtccgctcg cctcttcacg
     1741 cggatgagga ggatgggcgc cgcaaggatg aagagggtgg cggggtccag aacggccgga
     1801 ataaagaccc atgctcccag ggtgtccccg gcgaaaacgt aggccatgat ggagaagacg
     1861 atggacaata cgtggatcgt ggaagtcctg cggtgtgttg tctcaccccg tttctttttg
     1921 accatcctca gggtgtagtt gacgatgttc gctacgcaga caaggcagga aagacccgca
     1981 aaggccatcc cgatgtatgt catcatatca ccgctagtct atcggtccga caggcgcttg
     2041 tcaatgccgg aaccggagaa gatcgttcaa gaggttcaag aagaaggatc cggagctccc
     2101 tcggtgccag tccttgtgta taagccgcgc ccttgcgatg cactccagac ttcgtcattc
     2161 cggcctacgc aggaatgacg aaagatgttc ggatcgtccc cgaagaggca ggtcgcttcg
     2221 tcgtctgtct tgtccccccc cggggggggt tgtctcttct tgttccgtcc cgtgaagttt
     2281 tctatggcgg gatggtgcgt gttcgtctag aatgttaccg taaacgatgc ctccgcagat
     2341 agagatcatc ctgaacgggc taaaagagaa agtgcttgca ggcacgacca tcgccgacct
     2401 gataaagatg tccggtgagg aagacaagca tctcgtcgtg gagcgcaaca accgcttcat
     2461 ccacccccag acctaccatg caacgccgct ctccgaaggc gacaggatcg agttcataaa
     2521 ccccgacttc ggagggtaaa ggcggctcaa acgttctttg tcttgtggta ttcctcttcc
     2581 atttccatga ggttcttgcc gaaggcgatg gcgtgttttt ccatttcccg cgcggcggtt
     2641 tcggggtcgc cggctatgac cgcttcgacc gcggcgtcat ggagacccgc ggggtgaagt
     2701 ttctccgtgt cgggcttgac ggcctcgacg acctgcttcg tgagcctcat gacacaggtg
     2761 acgagggcct cgaagaaccg gttcccgcac atttcggcaa ggatgtagtg gacggtggtc
     2821 tttcggtcga cctcttcgga
//
LOCUS       DAOPOA010000257         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_38456_length_2835_cov_4.580935, whole genome shotgun sequence.
ACCESSION   DAOPOA010000257 DAOPOA010000000
VERSION     DAOPOA010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38456_length_2835_cov_4.580935"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PKJ17_06215"
     CDS             complement(<1..197)
                     /locus_tag="PKJ17_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75603.1"
                     /translation="MATKKASKTNQILEKKILAPSITMYKLYVPDIARKVKAGQFVVV
                     RGDDMGERIPLTVADYDAKKGT"
     gene            complement(236..1738)
                     /locus_tag="PKJ17_06220"
     CDS             complement(236..1738)
                     /locus_tag="PKJ17_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75604.1"
                     /translation="MAESVTILVVDDEKGIREGCRRILASEGYGVDVAENGKMALEMV
                     TGKPYDLILVDLMMPVMGGLELMEHVRQIDPGIILVVITGFATVETAVDAMKHGAYDY
                     IPKPFNPDQLMAVINRGLDKRRLTIEKEKLREERDQRLLEVASEKSKLHTIVNSMADG
                     ILVINREHQLVLWNPAVLKMLNVRGRVDESGVGMDFQKALKQQDLIDVINKAFSPDSS
                     QYTIISEEVVLAGETPKTLMVNVAGVRDERGENLGVVCTFRDISRLKEVEEIKSQFVR
                     MVAHEIRAPLGAIEGYLNAYLTGVAGTDPQFNRQMLERAKLRARSLMDLVNDLLLFAK
                     LESKKVVRKKELLDITEIIENTVELFRVQGSTKDVSFFVEIPEKLPLIEADRSEMEQL
                     FTNLVSNAMKYNVKNGKVTVRAVPEPHYLSISVSDTGIGMEEECLSCIFDEFYRVQGP
                     NTRYTTGTGLGLSIVKRIVESHFGRIEVESAVGKGTVFTVRLPLKQVNDK"
     gene            complement(1757..2158)
                     /locus_tag="PKJ17_06225"
     CDS             complement(1757..2158)
                     /locus_tag="PKJ17_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75605.1"
                     /translation="MAQKRILIIDDDVDFVDLNKAVLQNNGFEVETAFSAREGMDKVQ
                     FEAPDLILLDLMLEKHDTGFSFAKAIKADPRYKTIPILMISAVAGETGYDFTQEQDGY
                     WMKTDDFVAKPVEPEELVKRINALIDRAKQG"
     gene            complement(2130..>2835)
                     /locus_tag="PKJ17_06230"
     CDS             complement(2130..>2835)
                     /locus_tag="PKJ17_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75606.1"
                     /translation="NPLGTITIYAHILARSLEPGDPRKEDIDLIVTEANRTKEIVQGL
                     LSFARETKLKPGETNINDLLEETLGLLVNQVLFQNIKIKKVFNDDLPLLFADATQLKQ
                     VFLNIMLNAAQAMEGKGSLTLTTTHEGGTIEIRIRDTGPGIPPENIGKLFNPFFTTKE
                     KGTGLGLAISYGIVERHSGQIDVETELGKGSTFIITLPVDADAAGIIAPAKGLAANQN
                     INTGRRNHGTKKNSHH"
BASE COUNT          503 a          836 c          781 g          715 t
ORIGIN      
        1 gtacccttct tggcgtcata gtccgcaacg gtgagcggta tacgctcgcc catgtcgtcg
       61 ccgcggacga cgacgaactg gccggccttc actttcctgg cgatgtccgg gacatacagc
      121 ttgtacatgg tgatcgaagg agcaagaatt ttcttctcca ggatctggtt ggttttggat
      181 gctttcttgg tagccataac gtctcctttt taaaagttta attctatcaa aacaattact
      241 tgtcgttcac ctgcttgagc ggcagcctga cggtgaagac cgttcctttc ccgaccgcgc
      301 tttcgacctc gatgcgcccg aagtgggatt ccactattct tttcacgatg gaaagcccca
      361 atccggtgcc tgtcgtatag cgcgtgttcg gtccctgcac ccggtagaac tcgtcgaaga
      421 tgcaggagag acactcctcc tccatgccga tgcccgtgtc gctcacgctt atgctgaggt
      481 aatggggctc cggcacggcc ctgaccgtga ccttgccgtt cttgacattg tacttcatgg
      541 cattggacac aaggttcgtg aagagctgct ccatttccga cctgtcggcc tcgatgaggg
      601 gcagcttttc gggtatctcg acgaagaacg acacatcctt cgtcgaaccc tgaaccctga
      661 agagctcgac ggtgttctcg atgatctccg taatgtccag gagctctttc ttccttacga
      721 ccttcttcga ttccagcttc gcgaagagca gaaggtcgtt gacgaggtcc atgagcgacc
      781 ttgccctcag ctttgcccgc tccagcatct gccggttgaa ctgggggtcc gtacccgcca
      841 ctcccgtgag ataggcattg agataccctt cgatcgcccc cagcggcgcc cgtatctcgt
      901 gcgccaccat cctcacgaac tgggatttta tctcctcgac ctccttgaga cgggagatat
      961 cgcggaaggt gcagacgacg ccgaggttct cgcctctctc gtcgcgcact cccgcaacat
     1021 tgaccattaa ggtcttcggg gtctcccctg cgaggaccac ctcttcggag atgatcgtat
     1081 actgggaaga atcaggggag aacgccttgt tgataacatc tatcaagtcc tgctgcttta
     1141 gcgccttctg gaaatccatg cccactccgg actcgtcgac cctgccccgc acgttgagca
     1201 ttttgagcac cgccgggttc cacaggacaa gctggtgctc cctgttgatg acgaggatac
     1261 cgtcggccat cgagttcacg atggtgtgga gctttgattt ctcgcttgcc acttcgagga
     1321 gccgctggtc cctctcttcg cggagctttt ccttttctat ggtgagccgc ctcttgtcga
     1381 ggccccggtt gatgaccgcc atgagctggt ccgggttgaa aggcttgggt atgtaatcgt
     1441 aggcgccgtg tttcattgcg tcgacagccg tctccaccgt ggcaaaccca gtgatgacca
     1501 cgaggataat gcccgggtcg atctgccgca cgtgctccat gagctcaagt ccgcccatga
     1561 cgggcatcat gaggtcgacg aggatgaggt catagggttt ccccgtgacc atctcgagcg
     1621 ccatcttgcc gttctcggca acatcgacgc cgtacccctc tgacgcgagg atcctccggc
     1681 acccttcacg gatgcctttc tcgtcgtcga caacaaggat ggtgacagat tcggccatta
     1741 acgttcccgt ggagatctat ccctgtttcg ctctgtcgat gagggcgttg atcctcttga
     1801 ccagttcttc cggctccacc ggcttggcca cgaaatcatc cgtcttcatc cagtagccgt
     1861 cctgctcctg ggtgaaatcg taccccgtct cgccggcaac ggcggagatc atcaggatgg
     1921 ggatggtctt gtagcgcgga tcggccttga tggccttcgc gaaactgaac cccgtgtcgt
     1981 gtttctccag catgaggtcg aggaggatga ggtccggcgc ttcgaactgc accttgtcca
     2041 tgccctcgcg agccgagaat gccgtttcga cttcgaatcc gttgttctgc aggactgcct
     2101 tgttgagatc aacgaagtcg acgtcgtcat caatgatgag aattcttttt tgtgccatgg
     2161 ttccttctcc ctgtatttat attttgattt gccgctaatc ctttggccgg tgcaatgatc
     2221 cccgccgcat cggcgtcgac gggcaacgtg atgatgaatg tgcttccctt gcccagttcc
     2281 gtctcgacat ctatctgccc cgaatggcgc tccacgatgc cgtaggaaat ggcgagtccg
     2341 agccccgtcc ccttctcctt cgtggtaaag aaaggattga agagcttgcc gatgttctcc
     2401 ggcgggatgc ccggtcccgt gtcgcgtatc ctgatctcga tcgtgcctcc ctcgtgggtg
     2461 gtggtgagcg tcagcgagcc tttgccctcc atcgcctgcg ccgcgttgag catgatgttg
     2521 agaaacacct gcttgagctg cgtcgcgtcg gcgaagagca gggggagatc atcgttgaac
     2581 accttcttga tcttgatgtt ctgaaagagg acctggttga cgagcagacc gagggtctcc
     2641 tcgagaaggt cgttgatgtt cgtctcgccc ggtttgagct ttgtctcccg cgcgaagctc
     2701 aggagcccct ggacgatctc cttcgtcctg ttggcctcgg tcacgatgag gtcgatgtct
     2761 tccttcctcg ggtcaccggg ttccagggac cgtgccagta tgtgggcata gatggtgatc
     2821 gtgccgaggg ggttg
//
LOCUS       DAOPOA010000258         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_38476_length_2834_cov_3.261965, whole genome shotgun sequence.
ACCESSION   DAOPOA010000258 DAOPOA010000000
VERSION     DAOPOA010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38476_length_2834_cov_3.261965"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PKJ17_06235"
     CDS             <1..739
                     /locus_tag="PKJ17_06235"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOD75607.1"
                     /translation="MKKVYTRPKTMKEAHFHYCPGCGHGIINRLLMEVVDEMGLRESA
                     ICVAPAGCGMLVYNYFDIDTLESAHGRGAAVATGIKRVRPDNLVFSYQGDGDLAAIGT
                     AEGFHAANRGEPITVVFVNNGVYGMTGGQMAPTTLTEQVTTTTPNGRNPILAGHPVRI
                     CEVFSMLDGTAYLERVTVNKPAAVIKAKKAIKKAFQCQLDGKGFSLVEILSPCPTNWK
                     MGPVEACRWIDEVMTKQFPLGLVKEVS"
     gene            736..1278
                     /locus_tag="PKJ17_06240"
     CDS             736..1278
                     /locus_tag="PKJ17_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOD75608.1"
                     /translation="MTTNTIFSGFGGQGVLSMGFTLASAAMLEGRYVTYLPSYGVEVR
                     GGTANCTVVVSDEEIASPVASEPEFVVAMNQPSFNRFQNILQSGGLMCVNASLVNASS
                     VRSDIEVLSIPTSELAEKLGTIKVANMIMLGVLIRASNIISFDAMIKNLAELLGEGKS
                     KLIKLNREALETGFNYVKEQ"
     gene            1281..1709
                     /locus_tag="PKJ17_06245"
     CDS             1281..1709
                     /locus_tag="PKJ17_06245"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOD75609.1"
                     /translation="MVITQLSVSLENVPGALSHVSELLGREGVNIRAISVADTSDIST
                     VRFVVDDPEKAKNILKGNGFNPRETGVLAVETPDHPGGLLAVLKPLKAAGINVHYLYP
                     HLGRVSGNAIVILGVDRTEEAQKVLAQNWVKTLGKEVYSI"
     gene            complement(1838..2455)
                     /locus_tag="PKJ17_06250"
     CDS             complement(1838..2455)
                     /locus_tag="PKJ17_06250"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOD75610.1"
                     /translation="MRAERILWVCVACVLFAAVLRPIGTAHGGSAAFRDTIYDPGRLK
                     PLDSNPTLKVGDVAPDFTLPSITGNPVSLHDYRGRANVVISFVPAAWTPVCSDQWPGY
                     NIMKGLFDRNDAILLGITVDNIPTLYAWTRQMSEKGLWFPVLSDFYPHGAVASKYGIL
                     RTNGESERALFVIDRKGVIRYIDVHDINKRPRLEDLARALQNLQN"
     gene            complement(2466..>2834)
                     /locus_tag="PKJ17_06255"
     CDS             complement(2466..>2834)
                     /locus_tag="PKJ17_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOD75611.1"
                     /translation="VIIEILSFYCPVCQIHAPLVHEVYEKIEGRADLKEKIRMIGIAA
                     TNTAFEARSFKETYNIPFPVFSDEEGEIALLLGVKYTPTFIGVKVDGKGGQEQFYFLP
                     KVFTNPTRFIEDIMKTSGLE"
BASE COUNT          624 a          784 c          847 g          579 t
ORIGIN      
        1 aatgaaaaag gtatacacga gaccgaagac catgaaggaa gcccacttcc attactgccc
       61 gggttgtggt cacgggatca tcaaccggct tctcatggag gtcgtggacg agatgggcct
      121 cagggaaagc gcgatatgtg tcgcgcccgc ggggtgcgga atgctcgtct acaactattt
      181 cgacatagac acgctcgaat cggcccatgg ccgtggagcc gcggtcgcca ccggcataaa
      241 aagggttcgg cccgacaacc tcgtcttttc ctaccagggg gatggcgacc ttgcggccat
      301 cggcacggcg gaagggttcc acgcggccaa ccggggcgag ccgataacgg ttgtttttgt
      361 gaacaatggt gtttatggca tgaccggggg gcagatggcg ccgacaaccc tgacggagca
      421 ggtaacaacg acaacgccca acgggaggaa cccgatactg gcggggcacc cggtaaggat
      481 ctgcgaggtc ttttccatgc tcgacggaac cgcgtacctg gagagggtca ccgtcaacaa
      541 acccgcagct gtcatcaagg cgaagaaggc cataaagaag gcattccagt gccagcttga
      601 cggaaagggg ttctcccttg tcgagatcct ttcgccctgt cccacaaact ggaagatggg
      661 gcccgtcgag gcatgccgat ggatagacga ggtgatgacg aagcagttcc ccctgggact
      721 ggtaaaagag gtctcatgac gactaacacg atattttcag gtttcggcgg gcagggcgtt
      781 ctctccatgg ggtttacact cgcgagcgcc gcgatgctgg agggcagata tgtcacctat
      841 cttccgtcct atggcgtgga ggtgcgcggc ggcacggcaa actgcaccgt ggtggtctcc
      901 gacgaggaga tcgcttcgcc tgtggcatcg gagccggaat ttgtcgttgc catgaaccag
      961 ccttccttca accgcttcca gaacatcctc caatcagggg gtctcatgtg tgtcaacgcc
     1021 tctctcgtga atgcctcatc ggtgcggagc gacatagagg tcctttccat ccccacgagc
     1081 gagctcgcgg agaaactggg aacgataaaa gttgccaaca tgatcatgct cggggtgttg
     1141 atcagggcga gcaacatcat atccttcgat gccatgatca agaatctcgc ggagcttctc
     1201 ggcgaaggga agtcaaaact gatcaagctg aacagggaag ccctcgaaac gggcttcaat
     1261 tacgtcaagg agcaatgaca atggtgatca cgcagctgtc cgtcagtctc gagaatgtgc
     1321 ccggcgcgct ctcgcacgtg agcgagctcc tcggtcgcga aggcgtgaac atccgcgcca
     1381 tctccgtggc cgatacatcg gatatcagca ccgtccgttt cgtcgtagac gaccccgaaa
     1441 aggcgaagaa cattctcaag ggcaacggtt tcaaccctcg tgagacagga gtccttgctg
     1501 tcgagacccc cgaccacccg ggaggtctgc ttgccgttct caagcctctc aaggccgccg
     1561 ggataaatgt tcactatctc tatccccacc tgggaagggt gtcaggcaac gccatcgtca
     1621 tcctcggggt ggacaggacc gaagaggccc agaaggtgct ggcccagaac tgggtcaaga
     1681 cgctggggaa agaagtctac agcatctgaa gaagtcccag gtcacaggtt tcaggtctca
     1741 ggacaataag acggttgcgc gttgcgagtt gcgggttaga ggccagtaaa ggacagcaag
     1801 gcgcaaggat gccgaccttc tctcgtgata catcccgcta gttctgcagg ttttggagtg
     1861 cccttgccag gtcttcgagg cggggtcgct tgtttatgtc gtggacgtcg atgtatctga
     1921 tcacgccctt cctgtcgatt acgaagaggg cccgttccga ttccccgttc gtacgaagaa
     1981 tgccgtattt cgaggcgaca gcgccgtggg gatagaagtc ggagaggacc gggaaccaca
     2041 gtcccttctc ggacatctgc cttgtccagg cataaagcgt gggaatatta tcgacggtta
     2101 tgccgagaag tatcgcatca ttcctgtcga aaaggccctt cattatgttg tagccgggcc
     2161 actggtcgga gcagaccggt gtccaggctg ccggcacgaa ggaaatgacc acgttggcgc
     2221 ggccccggta atcgtggagc gaaacagggt ttcccgttat ggatggaagc gtgaaatccg
     2281 gggcgacgtc gcccaccttc aacgttgggt tggagtccag cggcttgagc cttccagggt
     2341 cgtagatagt gtctctgaag gccgcggacc ctccgtgtgc cgtcccgatc gggcgcagga
     2401 ccgcggcgaa gaggacgcat gcgacacaaa cccacaggat gcgctccgcc cgcattgtct
     2461 ggctcctatt ccagtcccga tgtcttcatg atgtcctcga tgaaccgggt agggttcgta
     2521 aaaaccttcg gcaggaagta gaactgttcc tgccctccct tgccgtccac tttcacgcct
     2581 atgaaggtgg gtgtgtactt gacgccgaga agaagcgcga tctccccctc ttcatcggaa
     2641 aagaccggga agggtatgtt gtacgtttct ttgaaggacc gggcctcaaa cgcggtgttc
     2701 gtggccgcta tgcctatcat tcttatcttc tccttcaggt ccgcgcgccc ctctatcttc
     2761 tcgtagacct catgaacgag aggcgcatgg atctggcaga cgggacagta aaaactgagg
     2821 atctcgatga tcac
//
LOCUS       DAOPOA010000259         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_38516_length_2832_cov_3.922218, whole genome shotgun sequence.
ACCESSION   DAOPOA010000259 DAOPOA010000000
VERSION     DAOPOA010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38516_length_2832_cov_3.922218"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PKJ17_06260"
     CDS             <1..483
                     /locus_tag="PKJ17_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75612.1"
                     /translation="KTRQMIHICAKRKVDAEEMARSLNFEEFLDRDVNAGFSGGEIKR
                     SELLQLKAQDPDLMLFDEPESGVDIENMALIGTTISQILEKDTPRAGTNVKPFVQQRS
                     ERTKMGFIITHTGYILDYVPADKGQVLYNGILACAGNPREIFQCIGRLGYEECVKCTV
                     "
     gene            471..1697
                     /locus_tag="PKJ17_06265"
     CDS             471..1697
                     /locus_tag="PKJ17_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOD75613.1"
                     /translation="MYSLEELEQRARQAVGKKAAFGDDVDLDSFDKSFVPHKYLAEEE
                     ICAIPEEEQQRLIMSGLDVTDTGRGGTYFQKDTTVVHCHSDEEGIEVMPVTEALKKHD
                     WVKEYFWKLVSVDQDKYTASAFLDLHDGYVIRALPGSKSIYPIQACLYLDKENLQQNV
                     HNLIIAEEGSELHIITGCATSPHMKRGVHVGISEFFVKKGAKLSFTMIHNWAEEMSVR
                     PRSVARVEEGGVFINNYICMKPVKSIQMYPTTHLVGDNATAMFYSVIVGTSGSYLDIG
                     GRIYLKNRGCRAEIVQRSISNGAHIINRGHLIGEAPDIKAHLECKGLLLSGGIIDSIP
                     QLEGHVEGVDMSHEAAVGKVAQEEINYLMSRGITEEEATSTIVRGFLSVDIKGLPTPL
                     KQEIDRAVDRSNKDAM"
     gene            1710..2597
                     /locus_tag="PKJ17_06270"
     CDS             1710..2597
                     /locus_tag="PKJ17_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOD75614.1"
                     /translation="MKRRDFLKLGVSALVASQLPRNLFGQAGQPLVSVCTGTDYAAVT
                     RKVITALGGMRAFVKPGNTVVVKPNMGWDRKPEYAANTHPLVVKTIVEECLASGARKV
                     KVFDNTCTDARRSYENCGIPAALKGMKNVDVRFVENDRFRKTALNGRFLKDWPLYDDA
                     LKADVLINVPIAKHHGLTGLTLGLKNMMGIMGDNRGYIHRGIEDALSDVNSVVKSHLV
                     VIDATRILTDHGPQGGDLKDVKVLNTVIASRDIVAADAYATTLFGMKPQDISTTVTAY
                     KRGLGEMDVKKMRVLSFRF"
BASE COUNT          709 a          803 c          795 g          525 t
ORIGIN      
        1 aagacgaggc agatgatcca catctgtgcc aaaagaaagg tcgatgccga ggaaatggca
       61 cggtccctca actttgagga attcctcgac agggacgtca atgccggttt ctcggggggg
      121 gagatcaagc gctcggaact acttcaactc aaggcccagg accctgacct tatgctcttc
      181 gatgaacccg aatcaggcgt ggacatcgag aacatggccc tcatcggcac cacgatctct
      241 caaatcctgg aaaaggacac tccgagagcg ggcacgaatg taaagccgtt tgttcagcaa
      301 cggagtgaac ggaccaagat gggcttcatc atcacccata cgggctatat cctggattac
      361 gttcccgccg acaagggcca ggtcctctac aacggaatcc tggcctgcgc gggcaatccc
      421 cgcgagatat ttcagtgcat cggacgatta ggctatgagg agtgtgtgaa atgtacagtc
      481 tagaagagct tgaacagagg gcccgtcagg cggtgggtaa gaaagccgcc ttcggggatg
      541 acgtcgacct cgactccttc gacaagtcct ttgtgcccca caagtacctg gccgaggagg
      601 agatatgcgc tatccccgag gaggaacagc agcgtctcat aatgtcgggg ctcgacgtga
      661 ccgacaccgg aaggggcgga acatatttcc agaaggacac caccgtcgtc cattgtcata
      721 gtgacgaaga aggcattgag gtcatgcccg tcacggaggc attgaagaaa cacgactggg
      781 tcaaggagta tttctggaag ctcgtttccg tggaccagga caagtatacg gcgtcggcct
      841 tcctggacct ccacgatggg tacgtcatca gggccctgcc cggaagcaag agcatctatc
      901 ccatccaggc ctgcctttac ctcgacaagg agaacctcca gcagaatgtc cataacctca
      961 tcatcgccga ggagggatcg gagctgcaca tcatcaccgg ctgcgcgacg tcgccccaca
     1021 tgaaacgcgg cgtccatgtg ggcatatcgg aattcttcgt caagaagggc gccaaactga
     1081 gctttaccat gatccacaac tgggccgagg aaatgagcgt ccgtccccgc tccgtcgccc
     1141 gcgtcgagga aggcggcgtc ttcatcaaca actacatctg catgaagccc gtcaaatcca
     1201 tccagatgta tcccacgacg cacctcgtcg gcgacaacgc cacggccatg ttctacagcg
     1261 tcatcgtcgg gacctcgggc tcctacctcg atatcggcgg aaggatctac ctcaagaaca
     1321 ggggatgccg ggccgagata gtgcagaggt ccatcagcaa cggcgcgcac atcataaacc
     1381 gcggccacct catcggggaa gcccccgata tcaaggcaca cctggagtgc aagggactcc
     1441 tcctgagcgg cggcatcata gattcgatac cacagctgga gggccacgtc gaaggcgtcg
     1501 acatgtcaca cgaggccgcc gtgggaaagg ttgcccagga ggagataaac tacctcatgt
     1561 cgcgggggat aacggaagaa gaggcgacgt cgaccatcgt tcgcggcttt ctgagcgtgg
     1621 atatcaaggg ccttcccaca ccgctgaagc aagagataga ccgcgccgtc gacaggagca
     1681 acaaggatgc gatgtgagga ggaccacccg tgaagcgcag ggactttctg aaactcggag
     1741 tttcggccct tgtggccagc cagcttccac gaaatctctt cggacaggcc gggcaacccc
     1801 tcgtctcggt ctgtacaggc acggactatg ccgctgtcac ccggaaggtg ataaccgcgc
     1861 tcgggggcat gagggccttt gtgaagccgg gaaacaccgt tgtggtcaaa cccaacatgg
     1921 gctgggacag aaaacccgaa tatgccgcga acacccaccc cctcgtggtg aagaccatcg
     1981 ttgaggaatg cctcgcttcg ggcgccagaa aggtgaaggt cttcgacaac acctgcaccg
     2041 atgcgcgccg ctcttacgag aactgcggca tcccggcagc cttgaaaggc atgaagaacg
     2101 tcgatgtccg gttcgtcgag aacgaccgct tcaggaaaac ggccctcaac gggcgttttt
     2161 tgaaagattg gcccctgtat gacgatgccc ttaaggccga cgtgctcatc aatgtaccca
     2221 tcgccaagca tcacggcctg acgggcctga ccctgggtct caagaacatg atggggatca
     2281 tgggggacaa caggggatac attcaccgcg ggatagaaga cgccctgtcc gacgtcaaca
     2341 gcgtcgtgaa aagtcacctt gtagtcatcg atgcgacacg gatcctcacc gatcacggcc
     2401 cccagggagg ggatctcaaa gacgtgaagg tcctcaacac ggtgatcgcc tcgcgggaca
     2461 tcgtggccgc ggacgcgtat gccaccaccc tcttcggaat gaaaccccag gacatctcca
     2521 cgacggtcac ggcatacaag cgcggactgg gggagatgga tgtgaagaag atgagggttt
     2581 taagttttag gttttaagtt ttacgtgaag gacgtgagga cgagaatagc tgtcccgttg
     2641 tcggcaattt tcttccccga tctcggctta gaacacaaag cctgaaacct agaactttta
     2701 ataggccaca tatatgatac ataaagagac ccaaatgttt acgacgtaca caaatgaggt
     2761 ttctaactta aaacgtaaaa cgtaaaacgg tctttatgag actccccgcc tcccgtatct
     2821 cccagcttct tt
//
LOCUS       DAOPOA010000260         2826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_38618_length_2826_cov_3.089498, whole genome shotgun sequence.
ACCESSION   DAOPOA010000260 DAOPOA010000000
VERSION     DAOPOA010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38618_length_2826_cov_3.089498"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..694
                     /locus_tag="PKJ17_06275"
     CDS             98..694
                     /locus_tag="PKJ17_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75615.1"
                     /translation="MGGRPAGLTVIELTIVVIIICIIVGLVIKGQSLIVKGQMKQIFK
                     QKQDIAAAFSRYYDHFAYFAGDDPVASSRYSGATNGNGNGRVGVGSSVTAPDFSCTGT
                     GTEQCDLWFELRQANLLGGTGFTNPRHVFSGNVALTYNAPGTTGAGHWLAFEGMPSDV
                     CRGLDKAYDDGVWNTGSIQGASDYDTARDGNFTLFFRL"
     gene            705..1184
                     /locus_tag="PKJ17_06280"
     CDS             705..1184
                     /locus_tag="PKJ17_06280"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOD75616.1"
                     /translation="MHIYFAHPCFNESQETFKREFLGRLRVALGQTDYGKAIGIVDPF
                     DDTPNIEGNRETKLKLSRTVKETCLRMLEDCDIIVALVDDGDTGVAFEAGYAHAINIP
                     IILVSKANCDEANAMLIGAARERIDNILQEEQIGKLARMFEWYYISKERDGHEPGKN"
     gene            1165..1602
                     /locus_tag="PKJ17_06285"
     CDS             1165..1602
                     /locus_tag="PKJ17_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75617.1"
                     /translation="MNPERIEADFYERTTIYGYRCNSCGYILDESKGGYLYLQNDRGD
                     RIPLKDHGEREIIAQMLRMEEESLHDCEALAASQEAMIDLMEERVGYLSACVCIDCLG
                     QFGLDLRKDALVCPHCRSEHIRTLLEMTDKRCPSCSTGRMERF"
     gene            complement(1828..2268)
                     /locus_tag="PKJ17_06290"
     CDS             complement(1828..2268)
                     /locus_tag="PKJ17_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOD75618.1"
                     /translation="MIEKSQWGEGLSREDMERFAQYLYVARAQPSEPIFNEGAIEPYM
                     CFVADGLVKVTKGDSKKNVKTICEIGPGRTVGEMSIIDGLPRSASAVAVEETTVLVLT
                     KENFEVLLNDNPKLGVALFRRLAQMISHRLRVSDWMLVEYIYES"
     gene            complement(2383..>2826)
                     /locus_tag="PKJ17_06295"
     CDS             complement(2383..>2826)
                     /locus_tag="PKJ17_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein oxidoreductase"
                     /protein_id="HOD75619.1"
                     /translation="GRIAGENAAGMNNAFEGVVGTAVTKIFDLEVARTGLSPLDAERE
                     GIDHFVSTIKGRSRSTAYPEGKPITVTYVAEKASGRLIGAQMAGNEGVAHRIDTLATA
                     LYNRMTLEDIARLDLAYAPPFATVWDPILVAANVALKRLQKERQL"
BASE COUNT          589 a          798 c          825 g          614 t
ORIGIN      
        1 cataaccgga tgaatgaggt tgccatggaa agtgcgaaag attcttgaca ctatcatgaa
       61 acgagaccaa aagaacgaaa ctttcaggat gtccggtatg ggagggagac ctgccggcct
      121 caccgtcatc gaactaacca ttgtcgtgat catcatctgc attatcgtag gtctcgttat
      181 aaaaggacag tcccttatcg tgaagggcca gatgaagcag atcttcaagc agaagcagga
      241 tatcgccgcc gccttctccc ggtattatga ccatttcgca tattttgccg gcgatgatcc
      301 ggtggcttcc tcccggtaca gcggtgccac gaacggcaac gggaacgggc gggtaggcgt
      361 tggttcgtcg gtgacggccc ctgatttttc ctgcacgggg acgggaacgg agcagtgtga
      421 cctctggttc gaactgaggc aggcgaatct cctcggcggg acaggattca cgaaccccag
      481 gcatgtattc agcggaaatg tggccctcac gtacaacgct cccggaacga cgggggcagg
      541 tcactggctg gcatttgagg gcatgccctc cgatgtgtgc aggggtctgg acaaggccta
      601 cgatgacggt gtctggaaca cgggcagcat acagggcgct tcggactacg ataccgccag
      661 agacggaaat ttcacactct ttttcaggtt gtgatgaggt gaccatgcat atctacttcg
      721 ctcatccctg tttcaacgaa tcccaggaga ccttcaagag ggaattcctg gggaggttga
      781 gggtcgcact gggacagacc gattatggaa aagcgatcgg catcgtcgat cccttcgacg
      841 acacgcccaa tatcgagggc aacagggaaa cgaagctcaa gctgagccgg accgtcaagg
      901 agacctgtct tcggatgctg gaggactgcg acatcattgt tgccctcgtc gacgacggcg
      961 acacgggggt ggccttcgag gccggttatg cccacgcgat caacatcccc atcatcctcg
     1021 tatcgaaggc aaactgcgac gaagccaacg ccatgctcat cggcgcggcg agggaacgga
     1081 tagacaacat actccaggag gagcagattg gaaagcttgc ccggatgttt gaatggtact
     1141 acatatcaaa ggagcgtgat ggtcatgaac ccggaaagaa ttgaggcaga tttctacgaa
     1201 aggaccacga tctacggata ccgctgcaat tcctgcgggt acatcctcga cgagtcgaag
     1261 ggtgggtatc tctatctgca gaacgacagg ggcgacagga taccgctgaa ggaccacggc
     1321 gagcgcgaga taattgccca gatgctccgc atggaagagg aatcccttca tgactgcgag
     1381 gcgcttgccg cctctcagga ggcgatgatc gatctcatgg aagagcgcgt gggttatctc
     1441 tccgcctgtg tgtgcatcga ctgtctcggc cagttcggcc tcgacctcag aaaagatgcc
     1501 ctcgtttgtc cccactgcag gtccgagcac atcaggaccc tgctggaaat gaccgacaaa
     1561 cgatgcccga gctgcagcac gggacggatg gaacgctttt agggttcgta tatggattta
     1621 gatgttgccg gacgacctga aggtttcccg ccattccggc aacgtactcc tcagtcatcc
     1681 ccccacttcc tcctccgcca ttccggctga aagccggggt ggcggaccgc gagggcccaa
     1741 ttccggagcc gggacgacga gtcttcaggt gtcaggaacg accgggactt ccagaactcc
     1801 cctgttgtcg cggacttggg acggcgtcta cgattcgtat atgtattcga cgagcatcca
     1861 gtccgacacc cgcaatctgt ggcttatcat ctgcgcgagt cttcggaaga gggcgactcc
     1921 gagcttgggg ttgtcgttca ggaggacctc gaagttctcc ttggtgagta cgaggaccgt
     1981 cgtttcttcc actgccaccg ctgacgcgga ccggggcagg ccatcgatga tgctcatttc
     2041 gccgaccgtt ctaccggggc cgatctcgca tatggtcttg acgttcttct tggagtcccc
     2101 cttggtgacc ttgacgagcc cgtcggcgac gaaacacatg tagggttcga tggccccttc
     2161 attgaagatg ggttcactcg gctgcgccct ggcgacgtag aggtactgcg cgaaccgctc
     2221 catgtcctca cggctcaatc cttcccccca ttgggacttc tcgatcatgg aaatacgttc
     2281 ttcaacggat gacaacgcgt ggtgcagcat ggcctctcct ggacctcagc attcttggat
     2341 ctgttaacag ttatgctctc acctggttgt atcaactgta tactatagtt gcctttcctt
     2401 ttgcaatctt ttcagggcca cgttggcggc gacgagtatc gggtcccata ccgtggcgaa
     2461 aggcggcgcg taggcgaggt ccaaccgggc gatgtcctcg agtgtcatcc tgttgtacag
     2521 ggccgtcgcg agcgtgtcta ttctgtgggc gacgccctcg ttgcctgcca tctgggcgcc
     2581 gataagcctg cccgatgcct tctcggccac gtaggtcacc gtgatcggct tgccctcggg
     2641 gtacgcggtg ctcctcgacc tgcccttgat ggtggacacg aaatggtcga tgccttctct
     2701 ttccgcgtcc aggggcgaca ggcccgtgcg ggccacttcg agatcgaaga tctttgtgac
     2761 ggccgttccc accacgcctt caaaggcgtt gttcatgccg gcggcgttct cgcccgctat
     2821 tcgacc
//
LOCUS       DAOPOA010000261         2816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_38813_length_2816_cov_2.526621, whole genome shotgun sequence.
ACCESSION   DAOPOA010000261 DAOPOA010000000
VERSION     DAOPOA010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38813_length_2816_cov_2.526621"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="PKJ17_06300"
     CDS             <1..1421
                     /locus_tag="PKJ17_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75620.1"
                     /translation="LTPRALSEITGSQDGNAVKQAHRRIMRSEVKSLRLPIRITCKNG
                     ETKWIHNCWVGVTWKGTPGVLCVFSDITESKKMEQQFLQAQKMEAIGQLAGGIAHDFN
                     NILTAISGYGNLLRIKTDNDPALNRYARNILACSNRAANLVKGLLAFSRKQVNDPRPT
                     DLHDVIKGAEKMLRRIIGEHIEFSVILSKTTPVVLADSNQIIQVLINLATNARDAMPK
                     GGKVTIRTETVENPDASAAGENGERQDGRYALLSFEDTGEGMDESMVDKIFDPFFTTK
                     EPGKASGLGLATIYGIVKQQKGFISVQTNVGSGTTFNIYLPLVDIPVDTREAIRLAAH
                     LRGTETILVCEDETDIRNFIREILESNGYTVIEAKDGEEGIDTFRRCRDKVALVLLDV
                     MMPKKSGKDVYDSIVELDPKVKTIFMTGYSADSLDKQGFGDAITPCIYKPIETDMLLT
                     EIRKKLDEEKIVQELKSSKVQG"
     gene            complement(1542..2300)
                     /locus_tag="PKJ17_06305"
     CDS             complement(1542..2300)
                     /locus_tag="PKJ17_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOD75621.1"
                     /translation="MTSPEEYFSRSIMLYGREGFEVLGKSTVAIVGLGGVGSWAAEAL
                     CRAGVGGIRLIDCDTVKATDVNRQLIALRTNEGVRKVDAAEDRLRQVNPEARLDPRHA
                     FFHHDTADDLLGGVDFVIDAIDSMNPKAELISQCIGRSLPIVSALGAASRTDPMKVRL
                     GLLTETEVCPLARTLRRYLRNRNVTTDIPVVYSTEKPLKPHPDGPAASRETTGAHVRG
                     RARESLPSLPTLPAIFGLIAANHVIMELVKKVQG"
     gene            complement(2297..>2816)
                     /locus_tag="PKJ17_06310"
     CDS             complement(2297..>2816)
                     /locus_tag="PKJ17_06310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOD75622.1"
                     /translation="AVGEIGLGLAPGMAPVEVQERAFVAQLDMAAGRGLPVLVHCRKA
                     HERMLDILRDYRGRVTVVLHSFSGSAEVMERFLEIGCYVSFSGSVTRERAKKYHRCAR
                     LVPAERFLLETDAPSIATQTTVASLVEPLHTREVAVKVAELRGVSLEEVCRISTESAC
                     RLFGLERMERER"
BASE COUNT          632 a          908 c          781 g          495 t
ORIGIN      
        1 agcttacccc ccgtgccctc tccgagatca cgggttccca ggacggtaat gccgtaaaac
       61 aagcccatcg caggatcatg aggtcggagg tcaagagtct caggcttccc atccggataa
      121 cctgcaagaa cggcgaaacg aaatggatac acaattgctg ggtcggcgtc acctggaaag
      181 gcacccccgg cgtcctatgc gttttttcgg acataacgga gagcaagaag atggagcagc
      241 aattcctcca ggcccagaag atggaggcca tcgggcagct cgcaggaggg atcgcccacg
      301 acttcaacaa catcctcacc gcgatcagcg ggtacggaaa cctgctcagg atcaagaccg
      361 ataacgaccc cgccctcaac aggtacgccc ggaacatcct cgcctgttcc aatcgcgccg
      421 cgaatctcgt gaagggcctt ctcgccttca gcaggaagca ggtcaacgac ccgcgtccga
      481 cagacctcca cgatgtcata aagggcgcgg agaagatgct tcgccgcatc atcggagagc
      541 acatcgagtt ctccgtcatc ctttcgaaga caacgcccgt ggtccttgcc gacagcaacc
      601 agataatcca ggtgctcatc aacctcgcga cgaacgcgcg cgatgccatg cccaaaggcg
      661 gtaaggtgac catccggacg gagacggtgg agaaccccga cgcgtccgcc gccggggaga
      721 atggcgaaag acaggacggc cgctatgccc tcctttcctt cgaggacacc ggggaaggca
      781 tggacgaatc gatggtcgac aagatcttcg atcccttctt cacgacaaag gaaccgggga
      841 aggcgagcgg cctcggcctt gccaccatat acgggatcgt gaagcagcag aagggattca
      901 tctcggtcca gacgaacgtc ggctccggca cgaccttcaa catctacctg cccctcgtcg
      961 atatccccgt cgacacccgg gaggccatac ggctggcggc gcacctcagg ggcacggaaa
     1021 ccatcctcgt ctgcgaggac gagacggaca tcaggaactt catcagggag atcctcgaat
     1081 ccaacggtta caccgtcatc gaggcaaagg acggcgaaga ggggatagac accttccgac
     1141 gttgcaggga caaggtggcc ctcgtcctcc tcgacgtgat gatgccgaag aagagcggga
     1201 aagatgtcta tgactccata gtcgaattgg atccgaaggt aaagacgatc ttcatgacgg
     1261 ggtactcggc ggacagcctg gacaaacagg ggtttggcga cgccatcacc ccctgcatat
     1321 acaagcccat cgaaacagac atgctcctca ccgagatacg aaaaaaactg gacgaagaaa
     1381 agatcgttca agagctcaag agttcaaaag ttcaagggta aaaaccaaca aagaaagcgg
     1441 gcaggcagat cagtccagga ttttcgcgtt cccggatgga acagccgtcg actacacgtc
     1501 tccttttctt ctgcttttac ccttgaaccc ttgaactttc tttatccttg aactttcttt
     1561 accagttcca taatgacatg gttcgccgcg atcaggccga agatggccgg gagggtgggc
     1621 aggctgggca aggattcgcg cgcccttccc ctcacgtggg cacccgtcgt ctcgcgggat
     1681 gcggccggac cgtccggatg gggcttgagc ggcttttccg tcgaatagac gacggggata
     1741 tccgtcgtga cattccggtt tctcaggtag cgcctcagcg tccgggcaag ggggcacacc
     1801 tcggtttcgg tgaggagacc gaggcgcacc ttcatggggt ccgtcctgct ggcggccccc
     1861 agcgcggaga cgatcggtaa cgagcgcccg atacactgac tgatgagctc cgccttcggg
     1921 ttcatgctgt cgatggcgtc tatcacgaag tcgacgccgc caaggaggtc gtcggcggtg
     1981 tcgtgatgga agaaggcatg gcggggatcg aggcgcgctt cgggattcac ctgccggagg
     2041 cggtcctcag ccgcgtccac cttccggaca ccctcgtttg tccttaaagc tatgagctgc
     2101 ctgttcacgt cggtcgcctt tacggtatcg caatcgatga gccgtatccc ccctacaccc
     2161 gcccggcaca gggcctcggc ggcccaggag ccgacgcctc ccagtcccac aatcgcaacg
     2221 gtgctcttcc cgagcacctc gaaaccttcc cgcccgtaga gcatgatgct ccgggaaaaa
     2281 tactcctccg gcgacgtcat ctttctcttt ccatcctttc caacccgaag agcctgcacg
     2341 cgctttccgt ggatatacgg cacacctctt ccagggagac acccctcagc tcggctacct
     2401 tcacggcaac ctcacgggtg tgcaaaggct caaccaggga ggccaccgtc gtctgcgtcg
     2461 cgatggaagg ggcgtcggtc tcgaggagaa accgctccgc cggcacgagc cgtgcgcagc
     2521 gatggtactt cttcgccctc tcccgagtca cggaaccgga aaaggagacg taacacccga
     2581 tctccagaaa cctctccatc acctccgcgc tccccgaaaa ggaatgcagc accaccgtca
     2641 ccctccccct gtaatctctc aggatgtcca gcatcctctc atgtgccttc ctgcagtgaa
     2701 caagcaccgg cagcccccta ccggccgcca tatcgagctg cgcgacaaag gcccgctcct
     2761 gcacctccac cggcgccatc cccggcgcga gaccaagccc gatctcgccg accgcc
//
LOCUS       DAOPOA010000262         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_38829_length_2815_cov_3.302899, whole genome shotgun sequence.
ACCESSION   DAOPOA010000262 DAOPOA010000000
VERSION     DAOPOA010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38829_length_2815_cov_3.302899"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1815)
                     /locus_tag="PKJ17_06315"
     CDS             complement(13..1815)
                     /locus_tag="PKJ17_06315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOD75623.1"
                     /translation="MSGIKDGMAQKTREDENLYQVLFESSEIATIIAERDGTILRVNK
                     RFLELSGYRRSDVVGKKKWSELVVNGDLTKMTQYRDFLDHDASAPAHTYEFKLAAKNG
                     STRDVQILRQAVAGTKHAAIHFVDISDLMRVGKKVISSEEKYRSLIESTDDSIYMIDR
                     EGTYLFVNRQVLARLCVTEANIIGKHYSDFHDAEDTIEFAGYVDKVFETGSSHRYEHR
                     SGKGGDRWMLRTLSPIVEGRTGQVRFVAVVSKEITDLKKTEEKLKYLSLHDPLTNLYN
                     RAYFEEEMHRHDNNRFDVVGLIMCDIDGLKLVNDTLGHDRGDELLTVASQVIRKSFRE
                     SDVVARVGGDEFAILLPNSPKSKVEEICARIRTSVAAYNKKNPHLPLGISTGFAIRTG
                     PGQSMAELYREADNAMYKEKLFGSQHARNVIVKNLLNTIESKGVTTKQSLKRLRQLVS
                     VLGSAAGLTEKRLKDLALLAQFHDIGNISIHDRILLKSGSLTEEELNEMRKHCDIGHR
                     IAQSAATLTPIADYILKHHEWWDGSGYPLGLTGEEIPLESRIMAIADAYESMTGGRPH
                     RKAVSREEALKELRRCAGTQFDPDLVKKFIKIVA"
     gene            complement(1850..>2815)
                     /locus_tag="PKJ17_06320"
     CDS             complement(1850..>2815)
                     /locus_tag="PKJ17_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOD75624.1"
                     /translation="GGNVAENAGYTRAVKYGVTRDYVRGLEAVLPSGDVIALGGKTVK
                     NVAGYDLISLLVGSEGTLAIITKIILRLLMRPHARRTVMAYLNDLPEAADLIVAVFRS
                     GIVPCAIELMDNIAINTVADHLGAALSRDAAALVLIETDGHGEEEVQAEALEITNLCR
                     RSPGVTEARLAADEAEAERLWTYRREVLPSLKALGKDHLEADVVVPRYNLPFLVRFTG
                     GLEHSDAIKIATYGHAGDGNLHVTILHRRRNFSELEEAYRLLELIYRETIAMGGSLTG
                     EHGVGLTVMDYLPMQIGGEALPLMRRIKAAFDPRGILNPGKIFTD"
BASE COUNT          484 a          827 c          842 g          662 t
ORIGIN      
        1 gagctccact tgtcatgcta ctattttgat gaatttcttt acgagatcgg ggtcgaactg
       61 agtgccggcg cacctgcgca gttctttgag cgcttcctcc ctggacaccg ccttgcggtg
      121 gggccgcccg ccggtcattg actcgtaggc atctgctatg gccatgatcc tgctttcgag
      181 gggtatctcc tcacccgtga gacccaaggg gtacccgctg ccatcccacc actcatgatg
      241 tttgagaata tagtccgcta tgggtgtcag ggttgcggcc gattgggcga tgcggtgacc
      301 gatatcgcag tgcttgcgca tctcgttgag ttcttcctcg gtgaggctgc ccgacttcag
      361 gagtatgcgg tcgtggatgc tgatattccc gatatcgtgg aattgcgcga gaagagcgag
      421 gtccttcagg cgcttctcgg tcaggccggc ggcgcttccc agtacgctta cgagctgtct
      481 caaccgtttg agggactgct tcgtcgtgac gcccttactc tcgatggtgt tcaggaggtt
      541 tttcactatg acattgcgcg cgtgctgact gccgaagagc ttttccttgt acatggcgtt
      601 gtccgcttcc cgatacaact ccgccatgct ctggccggga ccggtgcgga tagcgaaccc
      661 cgtggaaatg cccaacggga ggtgagggtt cttcttgttg tacgcggcca cgctggtcct
      721 gatcctggcg cagatctcct caacctttga tttggggctg ttcggcaaaa ggatagcgaa
      781 ctcgtcgcct ccgacgcgcg cgacgacgtc gctttcacgg aatgacttcc ggatgacctg
      841 tgaggcgacg gtgaggagtt cgtctcccct gtcgtgcccg agggtgtcgt tgacgagctt
      901 caggccgtct atatcgcaca tgatgagccc gaccacgtcg aaccggttgt tgtcgtgacg
      961 atgcatctcc tcctcgaaat aggcccgatt gtagagattc gtcaggggat cgtggaggct
     1021 caggtacttc agtttctctt ccgttttctt gaggtccgtg atctcctttg acacgacggc
     1081 aacgaacctt acctgcccgg tgcggccttc gactatgggg ctcagtgtgc gcagcatcca
     1141 gcggtccccg cctttcccgc tcctgtgttc ataacgatgt gacgatcccg tttcaaagac
     1201 cttgtccacg taccccgcga actcgatggt gtcttcggcg tcatggaagt cgctgtagtg
     1261 cttgccgatg atgttcgcct cggtcacgca caaccgtgcc agtacctgcc tgttgacgaa
     1321 gagataggtg ccttcgcggt cgatcatgta gatcgagtca tccgtggact cgatgaggga
     1381 acggtatttt tcctcgcttg agatgacctt cttgccgacg cgcatgagat cgctgatgtc
     1441 cacgaagtgt atggccgcgt gcttcgtccc ggcgacggcc tggcggagga tctggacgtc
     1501 cctggtggaa ccgtttttgg cagccaactt gaattcatag gtgtgagcgg gagcggaggc
     1561 gtcatggtca agaaagtcac ggtactgtgt catcttcgtg agatcgccgt tgacgacgag
     1621 ttcggaccat ttcttctttc ccaccacgtc ggaccggcgg taccccgata gctccaggaa
     1681 ccgcttgttc acgcgtaaga tcgttccgtc tctctccgcg ataatggtgg cgatctccga
     1741 ggattcgaaa agaacctggt acagattttc gtcttccctt gtcttctgtg ccatgccgtc
     1801 tttgatgcca ctcattatat caccggcgaa cgggcatttc caacactatt caatccgtga
     1861 atatcttgcc ggggttcagg atgccccggg ggtcaaaggc cgccttgatg cgcctcatga
     1921 gcggcaacgc ctcccctccg atctgcatgg ggaggtaatc catgacggtt aggccgacac
     1981 cgtgctcacc cgtgagggat ccacccatgg cgatcgtttc cctgtatatc agttccagga
     2041 ggcggtacgc ctcttcaagc tcggagaagt tcctccggcg gtggaggatg gtgacatgga
     2101 ggttgccgtc cccggcatgg ccgtaggtgg cgatctttat ggcatcgctg tgttcgagtc
     2161 cgcccgtgaa cctgacgagg aacgggagat tgtaacgggg gacgacgacg tccgcctcga
     2221 gatggtcctt gccgagcgcc ttgagcgatg gcaacacctc cctgcggtag gtccagagac
     2281 gctccgcctc cgcctcgtcc gcggcgaggc gcgcctccgt cacgcccgga gatcgccggc
     2341 acaggttggt tatctccagc gcctctgcct ggacctcctc ctccccatga ccgtccgttt
     2401 cgatgagcac cagcgcggcg gcgtcccttg acagcgcggc gcccagatgg tcagccacgg
     2461 tgttgatggc gatgttgtcc atgagttcga tggcgcaggg aacgatcccg gagcgaaaga
     2521 cggcgacgat caggtcggcg gcctccggca gatcgttgag atacgccatg acggtccggc
     2581 gggcgtgggg cctcatgagg agccgaagaa ttatcttcgt tatgatcgcg agcgtccctt
     2641 cggacccgac gaggagcgag atgaggtcgt acccggcgac gttcttcaca gtctttccgc
     2701 cgagtgctat cacatcaccc gaaggcagga cggcctcgag gccccggaca tagtccctgg
     2761 tgacgccgta cttgacggcc cgcgtgtatc ctgcgttctc ggcgacattg ccgcc
//
LOCUS       DAOPOA010000263         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_38854_length_2813_cov_5.868020, whole genome shotgun sequence.
ACCESSION   DAOPOA010000263 DAOPOA010000000
VERSION     DAOPOA010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38854_length_2813_cov_5.868020"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..844)
                     /gene="istB"
                     /locus_tag="PKJ17_06325"
     CDS             complement(86..844)
                     /gene="istB"
                     /locus_tag="PKJ17_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOD75625.1"
                     /translation="MEITTELKQTLKKLKLGGILATLPERGSYAKAQKLSHGEFLELV
                     LNDEIERREQGRTVRKMKSALLDQEQTFERFDWEAPITVDREKVKDLFSLGFTDRHEN
                     VIICGPVGVGKTHLANALGHSAVRKGLNVLMLRSQVLFKRLHQSRADHSFGKEVIGLI
                     RPHVLVIDDFGLQRLTGSEAQDLYEVMIERYQRSSTVITSSRPLDEWMGLFDDPLLAN
                     SLLDRLAHNAHQIFIEGESYRKRMGRTKKATERD"
     gene            complement(846..2390)
                     /gene="istA"
                     /locus_tag="PKJ17_06330"
     CDS             complement(846..2390)
                     /gene="istA"
                     /locus_tag="PKJ17_06330"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOD75626.1"
                     /translation="MARKEYMVVDVVDILRRLQNGYSIRAVTRATGMDRNTLRKYLRL
                     AYKKGFGKDGPCDLETIAGEVVREIQSRPPGPQRSLDQVLLPHKAAVESWIKDDHLTL
                     TKVHILLSRRGVEVSYPGLYRFVSVHIGIPSKTTVRMADTAPGEVAEVDFGKLGLLYD
                     EATARRRSVYGLVVTLCSSRHQYVHVTFTQDLPSLIFGMEDAWVFFGGLTRKVVIDNL
                     KAAVVRSDRYAPVFNRTFLEYSRFRGFIIDPARPDDPTGKPKVEREVPYVRKNFFAGE
                     TFRDLAHVQEEAVRWCRDTAGMRIHGTTRKRPLIVFNEEEKDALLPLTGERFDVPRWG
                     SVKVHPDHHIRFGNSLYSVPTRYVGREVSVRKDTRLLRIYYKGDLVKTHPSVPRGKRS
                     TDFKDYPKEKAPYAMRSCTYYVDRAGQIGPSARRFAELLLSGTFPWTKLRQAQKLVRL
                     PDRYGKERVEKACERSLAFELVDVRRVEEIIQQGLVREDTEERRREPLPSRFARPAAY
                     FVKKEE"
BASE COUNT          542 a          857 c          795 g          619 t
ORIGIN      
        1 ttaattcacc cacccccttt tcatgttaat tcacccgggg gcggcgcaat atcctcagtc
       61 ttctaccatg accatctcct catgttcaat ccctttcggt tgcctttttg gttctgccca
      121 tcctcttccg gtagctttcc ccttcgatga agatctggtg ggcgttgtgg gccagcctgt
      181 caaggaggga gttggccagg agtggatcat cgaagagtcc catccattca tccaggggcc
      241 ttgagctcgt gatgacggtt gaggaccgct ggtaccgctc gatcatcacc tcgtacaggt
      301 cctgtgcctc cgatcccgtc agcctctgca ggccaaagtc atcgatgacg agcacgtgag
      361 ggcggatgag cccgatgacc tccttcccga acgagtggtc ggccctcgac tggtggagtc
      421 ttttaaagag aacctgggac ctcagcatca gcacattgag gccctttctc accgcactgt
      481 gtcccagggc attggcgagg tgggttttcc ccacgccgac ggggccgcag atgatgacat
      541 tctcatgccg gtcggtaaag ccgaggctga agaggtcctt caccttctcc ctgtcgacgg
      601 tgatcggggc ctcccagtcg aaccgctcga aggtctgctc ctggtcgaga agggcgcttt
      661 tcatctttct cacggtcctg ccctgctcgc gtctttcgat ctcatcgttg agcacgagtt
      721 ccaggaactc gccgtgagag agcttttggg ccttcgcata cgaccctctt tccggcaggg
      781 tggcaaggat gcccccgagc ttcagtttct tgagcgtctg tttcagttcc gttgtgatct
      841 ccatctcatt cctccttctt cacgaagtac gctgccggcc gggcaaaccg ggacggcaac
      901 ggctcccgcc ttctttcctc ggtgtcctcc ctgacgaggc cctgctgtat gatctcctcg
      961 accctgcgca cgtcgacgag ttcaaaggcg agggaccgtt cacatgcctt ctccaccctc
     1021 tctttgccgt accggtcggg cagcctcacg agcttctgcg cctggcgcag ctttgtccag
     1081 gggaaggtcc ccgagaggag gagctccgca aagcgccgtg ccgaggggcc gatctgcccc
     1141 gccctgtcga catagtaggt gcagctcctc atcgcatagg gcgccttttc cttcgggtag
     1201 tccttgaagt ccgtcgaacg cttcccccgg ggaacggagg gatgggtctt gacgagatcg
     1261 cccttgtagt agatgcggag aagtctcgtg tcctttctca ccgacacctc cctgccgaca
     1321 taccgggtcg ggacggagta gaggctgttg ccgaagcgta tgtggtggtc gggatggacc
     1381 ttcaccgatc cccagcgggg gacatcgaac cgctctcccg tcaggggaag gagggcatcc
     1441 ttctcctctt cattgaagac gatgagcggc ctcttcctcg tggtcccgtg gatcctcatt
     1501 cccgcggtgt cacggcacca cctgaccgcc tcctcctgga catgggcgag gtccctgaag
     1561 gtctccccgg caaagaagtt cttccttaca tagggcacct cccgctccac cttcggcttg
     1621 cccgtcgggt catcgggccg ggcgggatcg atgataaaac ccctgaaccg ggagtactcc
     1681 aggaaggtcc tgttgaagac aggggcgtag cggtcggatc tgaccaccgc cgccttcagg
     1741 ttatcgatca cgacctttct cgtcaacccg ccgaagaaga cccaggcatc ctccatgccg
     1801 aagatcaggg acgggaggtc ctgggtgaag gtcacgtgca cgtactggtg cctgctcgaa
     1861 cagagtgtga ccacaagtcc gtagacagac cgtctccttg cggtcgcttc gtcgtagagg
     1921 agacccagtt tgccgaagtc cacttccgcc acctcccccg gtgcggtatc cgccatgcgc
     1981 accgtggtct tcgatggaat gccgatgtgg acggacacga accggtacag gcccgggtag
     2041 ctcacctcga caccccgtct tgaaagcagg atatggacct tcgtcagcgt gagatgatcg
     2101 tcctttatcc acgactcaac cgctgccttg tggggaagga ggacctgatc aagcgacctc
     2161 tgcggaccgg gcgggcggga ctggatctct ctgacgacct ccccggctat cgtctcgaga
     2221 tcgcatggcc cgtccttgcc aaaacccttc ttgtaagcca ggcgcaggta cttccggagg
     2281 gtgttgcgat ccatgcccgt cgccctggtg accgccctga tcgaataacc gttctgaagc
     2341 cttctcaaga tatcgacaac atcaaccacc atgtactcct ttctcgccat accgcctccc
     2401 gttcgacaaa gagataacgg gagtagtatg cgctgatttc ggcgccgacc gggtgggtga
     2461 attaacttga aaaagtgggt gagagatctt gaaaaagggg tgggtgaacg atcttgaaaa
     2521 acagaccgtc taaatgggtg agctatctag aaaaatcaca gttgtctcgc gcgatccccc
     2581 gacatcacag tcgtgtcggt ccaaagcaac cggtaccctt aatactgtga ggatgcctga
     2641 accttcgtcc gccccggcgc tcaggcgtct aaattgtctc cgaattgaca gggctaagca
     2701 agagaaaagt ggagaaccgg tgcctgcctg cgggagagaa gaatgagggc gccgaagaaa
     2761 cagcgcggga caaagtatcc gttttctggt aagaaattgg aaaggagagc ttc
//
LOCUS       DAOPOA010000264         2809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_38926_length_2809_cov_3.607480, whole genome shotgun sequence.
ACCESSION   DAOPOA010000264 DAOPOA010000000
VERSION     DAOPOA010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38926_length_2809_cov_3.607480"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..2002
                     /gene="selB"
                     /locus_tag="PKJ17_06335"
     CDS             110..2002
                     /gene="selB"
                     /locus_tag="PKJ17_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOD75627.1"
                     /translation="MKRIIVGTAGHIDHGKTTLIRALTGIDCDRLKEEKERGITTELG
                     FAHWHLDDDLLVGIVDVPGHERFVRHMVAGAWGIDTVLLVVAADEGVMPQTREHLDIC
                     EMLGLTKGIVVITKKDLVDEDMVELVEEDIRDFLKGRPLEDAPVVAVSATTGENLDLL
                     KGMLRDVSTRIEEKSRGGIFRLPIDRVFTLRGLGTIVTGTCISGSLSVGEEVELFPFG
                     RKARVRSIQAYHEDVKEASAGQRIALNLQGLDRQDVERGSMVARPDTLLLTSRIDASL
                     RLLKLPIKPIRHDSILRFHIATTQEEARLVLLESDQIEPGQELFVQFVFKKPVVVLPG
                     DRFILRGSYAIQTVGGGRVLDIMPARHKRKSGDLGGIYRVLSEGTDLEKAGYHVLKGG
                     FQGVRQQSLSVLTGKDMPSIERIGEVLQKEAVATLVGRTYLHKDYFDVYQEKIIAFVG
                     EYHRANPLKIGIPKEELRSRLPATDVQVFQAALDECVTSGLIIVEKDRVRSRDHGQGT
                     SQTDEFEKRVLEALLSAGFTPPGLKDLAGIVGVTEKNTRELLERLAFRGGVVKVGQDM
                     YFHRDMIEEVKEKATNYLRNHQEMGPSDFKSELNLSRKFLIPLLEYLDQIKLTIRKGD
                     KRVLRG"
     gene            complement(2231..>2809)
                     /locus_tag="PKJ17_06340"
     CDS             complement(2231..>2809)
                     /locus_tag="PKJ17_06340"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOD75628.1"
                     /translation="RSPAPPLEYVAHSANDTFFSSGSRQHLKPVFITLLILLFIFIGA
                     AGYRFVLATKQPQDTVIIKEVEKPETPREPAVENPQPKDQTETAEVKPPAQTPAEAAP
                     EKDVIAAFKEEMKKKHETSRNRVAVVKASAANVRSGPGLESPRILTIVQGEILPILDE
                     RSDSTGMKWYKVSLYGNRSGWIASQVATATTK"
BASE COUNT          613 a          740 c          842 g          614 t
ORIGIN      
        1 tcgctatgat ggtaccccag tttttgttcc tgacacaaag aatgatacat taccactgcc
       61 tttcccccct ttccctgcgg tcccattcgt ggtatagtct tagcctgtca tgaaacgtat
      121 aatcgtagga accgcaggtc atatcgatca tggtaagacg accctgatca gggcgctcac
      181 gggtatcgat tgcgatcgtc tcaaggaaga gaaggaaagg ggcatcacga cggaactcgg
      241 ttttgcccac tggcatctgg atgacgacct gcttgtcggt atcgtcgatg tcccgggaca
      301 tgaaagattc gtgcgtcaca tggttgcggg cgcctggggc atcgatacgg tgcttctcgt
      361 tgttgccgcc gatgaagggg tcatgccgca gacccgggag catctggaca tatgcgagat
      421 gctggggctc acgaagggca tcgtcgtcat aaccaaaaaa gaccttgtcg acgaggacat
      481 ggtcgagctg gtggaagagg acatcaggga tttcctcaag ggcaggccgc tggaagatgc
      541 tcccgtggtg gcggtgtcgg cgacgacggg ggagaacctg gatctcctaa agggcatgct
      601 tcgcgatgtg tcgacccgga tcgaagagaa atcgagaggc ggcattttcc ggctgcccat
      661 cgacagggta ttcacccttc ggggcctggg gaccatcgtc acggggacct gcatttcagg
      721 gtccctatcc gtgggcgagg aagtggagct gtttcctttc ggcaggaagg cgagggtgcg
      781 cagcatacag gcttatcacg aggacgtcaa agaggccagc gccggccaga ggatcgccct
      841 taacctgcag ggccttgaca ggcaggacgt cgaaagggga agcatggtgg cgcggcccga
      901 cacgcttctg ctcacgtcgc gcatcgatgc ctcgctgcgg cttctgaagc ttcccataaa
      961 accgatacgc cacgacagca tattgcgctt tcatatcgcg acgacgcagg aggaggcgcg
     1021 cctcgtgctg cttgagagcg atcagataga gccggggcag gaactcttcg tccagtttgt
     1081 tttcaagaaa cccgtcgtgg ttcttcccgg tgacaggttc atcctgcgcg gttcctatgc
     1141 catccagacc gtgggcggcg gcagggtcct tgacatcatg cccgcgagac acaagagaaa
     1201 gtcaggtgac ctggggggta tctaccgcgt cctcagcgaa gggacggatc tggagaaggc
     1261 cgggtatcac gttttgaagg gcggttttca gggtgtcaga caacagagcc tgtccgtgct
     1321 taccggcaag gatatgcctt ccattgagag gatcggggaa gtcctccaga aagaggccgt
     1381 ggcgaccctc gtcggcagga cgtatctgca caaggattat ttcgacgtat atcaggaaaa
     1441 gatcatcgcc ttcgtggggg aatatcacag ggcgaatccc ttaaagatcg gcatcccgaa
     1501 ggaggaactc cggtcgcgcc ttcccgccac cgatgtccag gtgttccagg cagcgctcga
     1561 tgaatgcgtc acctccggcc tcatcatcgt cgaaaaagac cgcgtcaggt cgcgggacca
     1621 cggccagggc accagtcaga ccgatgaatt tgaaaaacgc gttctcgagg ccctcctgtc
     1681 ggcgggattc actccccccg ggttgaagga ccttgcgggg atcgtgggag tcaccgaaaa
     1741 gaacacgcgg gaacttctcg agcgccttgc cttcaggggt ggtgtggtga aagtgggcca
     1801 ggatatgtat ttccaccgcg atatgatcga agaggtaaag gaaaaggcca cgaactatct
     1861 ccgcaaccac caggaaatgg gcccctcgga cttcaaatcc gaactcaatc tctcaaggaa
     1921 gttcctcata cctctccttg aataccttga ccagatcaaa ctcacgatca ggaagggaga
     1981 caagagggtg ttaaggggat agagacggtt ttaagtttta ggttttaagt ttcaagtaaa
     2041 agaaacaaga aaagaacggg tccgtttgaa atccgttggc atcgagcgtc agcttctcaa
     2101 gttttctgaa accatcttaa gtcccaaatg ccaagcttta agttgcagac gagaagaacc
     2161 aatctgtcgg gaatggtccg gccagccgtt cttccatctt ttccttaaaa cctaaaacct
     2221 aaaacggtct ctattttgtc gttgccgttg ctacctggga ggcgatccag cctgagcggt
     2281 tgccatagag gcttaccttg taccacttca ttcccgtgct gtcgctgcgt tcatcgagga
     2341 tgggcaggat ctcgccctga acgatggtga ggatccgcgg ggactccaga ccgggaccgc
     2401 tgcggacgtt ggccgcagac gccttgacga cggcgacacg gttccgggac gtttcgtgct
     2461 tcttcttcat ctcttccttg aaggcggcta tcacatcctt ttcgggggct gcctcagcgg
     2521 gagtctgtgc cggaggcttc acctcggcgg tctccgtttg gtcttttggc tgcggattct
     2581 caacagcggg ttccctcggt gtctccggtt tctcgacctc ttttatgatg acggtgtctt
     2641 gcggttgctt cgtggcaagg acgaaacggt accctgccgc tccgataaag atgaaaagca
     2701 gtatgagcag cgttatgaat acgggtttca ggtgctgtcg ggagccggaa ctgaagaagg
     2761 tgtcgttggc actgtgcgcg acgtattcca gcggaggcgc cgggctgcg
//
LOCUS       DAOPOA010000265         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39045_length_2803_cov_2.598981, whole genome shotgun sequence.
ACCESSION   DAOPOA010000265 DAOPOA010000000
VERSION     DAOPOA010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39045_length_2803_cov_2.598981"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKJ17_06345"
     CDS             complement(<1..295)
                     /locus_tag="PKJ17_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOD75629.1"
                     /translation="MAEGHDSKSMKMTPQRMAIMKYLDGNTCHPSAADIHGAIREQFP
                     TMSLATVYNTLETLKERGAVIELSIDRLKKRFDPNPEPHHHLMCIKCKAIVDVF"
     gene            complement(399..923)
                     /locus_tag="PKJ17_06350"
     CDS             complement(399..923)
                     /locus_tag="PKJ17_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOD75630.1"
                     /translation="MNDKERLNALETALGNEMKEREFYLRNADRTQNPLGKAMFRRIA
                     EDELEHYQRLKELHEKWAAEGKWPETVPLVVNTTNIKEVLVNTLKAIDPSAPTDTGDL
                     EAVKIAADFEDKGVRFYRELAAASADKRERDFFELLAMVEYEHFLSLRDAEEYFENPA
                     TWFTKVEHPMMDGG"
     gene            complement(945..1439)
                     /locus_tag="PKJ17_06355"
     CDS             complement(945..1439)
                     /locus_tag="PKJ17_06355"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOD75631.1"
                     /translation="MAKERGADHAVIVDTSSVSTAPWVRMKCRFGCPGYGLRLSCPPH
                     TPEYDRTRSMLDSYDRAILLHYHWVKGTNSVTMLNDTVVDLETTLFLDGFYKAFGMGC
                     GFCTLCKKCDTTKSCVHPERMRPSMESCGIDVYQTARANGLPIEVVRTHAQDRDVYGI
                     VLID"
     gene            complement(1655..2656)
                     /gene="epmA"
                     /locus_tag="PKJ17_06360"
     CDS             complement(1655..2656)
                     /gene="epmA"
                     /locus_tag="PKJ17_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOD75632.1"
                     /translation="MQYRLAHKTMAITSIQDIHHILTKRHELLRATRDFFYERGYLEV
                     ETANLMATAPPDPHIDPLHVQISGKGPFFLHTSPEMGMKKLLRSGHRRIFQVCKVWRV
                     EDHQEMHNTEFTMVEWYREGTYREAMHETEDLVAFIARRLLHPVPQHLLPPYPLHELG
                     EVFLKRMGFDPFPLDRGAFFDALRERGFPGIDDRDDWNSLFFKALIQEIDDAIRSPRP
                     SLVAGWPFSISTMAKRRDDNAAVVERFELYIDGLEIANGYTELVDPAQQRERFEEDNR
                     QRAGMGKRTFPVDETFLNALKTIDTSCAGVSVGLDRLLMALLEKKTIGDVIPDRLLV"
     gene            2702..2794
                     /locus_tag="PKJ17_06365"
     tRNA            2702..2794
                     /locus_tag="PKJ17_06365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2737..2739,aa:Ser,seq:gct)
BASE COUNT          557 a          791 c          792 g          663 t
ORIGIN      
        1 taaagacgtc aactatggcc ttgcacttga tgcacatgag atgatgatgg ggttccgggt
       61 tcggatcaaa acgtttcttc aaccggtcta tggaaagctc gatgactgcc ccccgttcct
      121 tcagtgtctc caatgtgttg tacacggtgg ccagggacat ggtggggaac tgttccctga
      181 tggccccgtg tatgtctgcc gcactggggt ggcacgtatt gccgtcgagg tatttcatga
      241 tcgccatcct ctggggtgtc atcttcatgc tcttgctgtc gtgaccctct gccattccaa
      301 taaaagccct taacgaatac actatactaa ctaataacca ttcttgtcaa gtattaatta
      361 taaaatactt gtctccaatg gaaaggcgtg ttcgttcatc acccgccgtc catcatgggg
      421 tgttcaacct tggtgaacca cgttgccgga ttctcgaaat actcttcggc atcgcgcagc
      481 gacaggaagt gttcgtattc gaccatcgcg aggagttcga agaagtccct ctcccgtttg
      541 tccgctgagg ccgcggcgag ttccctgtaa aaacgcacgc ccttgtcctc aaaatcggcg
      601 gcgatcttca ctgcttcgag gtcccctgta tcggtcgggg ccgacgggtc gatggccttg
      661 agggtgttga cgaggacctc ctttatgttt gtggtgttca caacaagagg cacggtttcg
      721 ggccatttgc cttcagcagc ccatttctca tggagctcct tcaggcgctg ataatgttcg
      781 agctcatcct cggctatgcg cctgaacatc gccttgccca gggggttttg cgtgcggtcc
      841 gcattcctga ggtagaactc gcgctccttc atctcgtttc caagcgctgt ttccagcgcg
      901 ttcagcctct ccttgtcatt catggcgtca cccttgaccc tccttcagtc gataagaaca
      961 atgccatata catcccggtc ttgagcgtgg gtacggacga cctcgatggg aaggccgttc
     1021 gcacgtgccg tctgatagac atctatgccg caggattcca tggagggcct catgcgttcg
     1081 ggatgcacac agcttttggt cgtgtcgcac ttcttgcaca gggtgcagaa cccacagccc
     1141 atcccgaagg ccttgtagaa cccgtccagg aaaagtgtcg tctccaggtc gaccaccgtg
     1201 tcgttgagca tggtcaccga gttcgtcccc ttcacccagt ggtaatggag gaggatcgcg
     1261 cggtcatacg agtcgagcat cgaacgtgtt ctgtcgtact cgggggtatg aggcggacag
     1321 gacaatctga ggccgtatcc ggggcacccg aagcggcact tcatccgcac ccatggggcc
     1381 gtggagacag aggaggtgtc cacgatgacc gcatggtccg ctcccctctc cttcgccatc
     1441 tccacatatc tcgaaagatc gccattacgc ataagttacg taaactatag gacagccggc
     1501 gggattggtc aaggcagcaa aacggtggca accgcttgaa ccgcttgaac cgcttgaaag
     1561 ttcaaaaaaa gacagagaag gggccagtaa tgaagaagcc ggaagtctct aacgctcaag
     1621 cggcttaagc cgttcaagcc gttcaagcgg tcttttagac gagcagcctg tccggtatca
     1681 cgtcccctat ggtcttcttt tccaggaggg ccatgaggag cctgtcgagg ccgacggaga
     1741 cgccggcaca ggaggtgtct atcgttttga gggcgttcag gaaggtttcg tcgacgggga
     1801 aggtccgttt tcccattccg gcccgttgcc tgttgtcttc ctcgaaccgt tcccgctgct
     1861 gcgctggatc gacgagttcc gtgtatccgt tagctatctc gaggccgtca atgtacagct
     1921 cgaaccgttc cacgacggct gcgttgtcgt cccgcctctt tgccatcgtg gagatggaaa
     1981 agggccaccc cgcgacgagg ctgggcctcg ggcttctgat ggcgtcgtcg atctcctgga
     2041 tgagcgcctt gaagaagagg ctgttccagt catccctgtc gtcgatgccc gggaagcccc
     2101 tctccctgag ggcgtcgaag aaggcgccgc ggtcgagggg aaaagggtcg aaccccatcc
     2161 tcttcaggaa gacctcgccg agctcgtgca gcgggtaggg cggcaagagg tgttggggca
     2221 cggggtggag gagccttcgg gcgatgaatg ccacaaggtc ttcggtttcg tgcatcgcct
     2281 cccggtacgt gccttcccgg taccactcca ccatggtgaa ctccgtgttg tgcatctcct
     2341 gatggtcctc gacgcgccag accttgcata cctggaatat ccgcctgtgc cccgagcgaa
     2401 ggagcttctt catccccatc tcgggtgacg tgtggagaaa gaacggtccc tttcctgaga
     2461 tttggacgtg aagagggtcg atatgcggat ccggcggcgc cgtggccatg aggttcgccg
     2521 tctccacttc gagatacccc cgctcgtaga agaaatcgcg cgtcgccctg aggagttcgt
     2581 ggcgtttcgt cagaatatgg tggatgtcct gtatgctcgt gattgccatc gtcttgtgcg
     2641 cgagtcgata ttgcatttta tacagcaaac aagtatactt tgtccatctt aaggcttccg
     2701 cggagaggtg accgagtagg ccgaaggtgc tcgcctgcta agcgagtgta cggataaaac
     2761 cgtacccagg gttcaaatcc ctgcctctcc gccatcaaaa aaa
//
LOCUS       DAOPOA010000266         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39054_length_2802_cov_3.926101, whole genome shotgun sequence.
ACCESSION   DAOPOA010000266 DAOPOA010000000
VERSION     DAOPOA010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39054_length_2802_cov_3.926101"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PKJ17_06370"
     CDS             <1..562
                     /locus_tag="PKJ17_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HOD75633.1"
                     /translation="GRPSIPFTLIADEYGSYAVEGFDTILAQGRSLGFGVIISVQELA
                     SLFKTSETDAKRLIGNTNYKIVMKVEDTDTAKFLAERAGESFFMMPNIRNDSKMMESL
                     GNWDGTYAFQKGSRVEMRDLAGLKVGEGYIFAGDEVRRFRTRYIPPAGNVKQLRLMKY
                     VKKTTLGTLQERRSTDLRCRDTGRSG"
     gene            complement(522..776)
                     /locus_tag="PKJ17_06375"
     CDS             complement(522..776)
                     /locus_tag="PKJ17_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75634.1"
                     /translation="MTELITKLIGTTQTMVSPLMPVLGTFLIIKGLIRLANGNEAAKG
                     GAGGGKMIIIGAFLTCYKLTSWIVISTLTRCGLYLGNAGL"
     gene            875..1564
                     /locus_tag="PKJ17_06380"
     CDS             875..1564
                     /locus_tag="PKJ17_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75635.1"
                     /translation="MAVFGKQKKEAPDPDGGEALAIEIGIFRGLKLTSRALLVIPVAT
                     VVLSIIMVVASIVIVVAGKGRDKIVFMDSTGRPSLVRADDPDKIHWPELEIFCRDAIR
                     DILGWTYVEVRSQSDLANRISRVSKRFDPASFKAFYEPYRDNYLKSLSDQKLIVTAEW
                     NALKDSKIRGKEAVVLASVRLNAVRVVGEEAASEDLKLKTYEIKLYKGARSVENPFGL
                     YIVRFSEPAGG"
     gene            1557..2357
                     /locus_tag="PKJ17_06385"
     CDS             1557..2357
                     /locus_tag="PKJ17_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75636.1"
                     /translation="MAEAAPDMPQETPKQQAEGQIVVPPFDLPEKDIVEMKEKVQRLK
                     PLIEKDHELARARERDVDEVPGAVPTIHLSYGYGSVINLPYSFSVDDIAIGAREKFNI
                     ETRGNSLIIFPVREFKSTNIIVFERTQAGDQLPHHYLLVENGASGEADLTVNVRRSGM
                     GGVSDITDAMVRVITTQRIPEKGTAEDILLEGRSPSLTNLSDYPFIRMMKLQKPELHV
                     FMIAGKVMPVGDADFALDLGNGTSVVASRSREITVRRVGDGKTFRNSR"
     gene            2329..2601
                     /locus_tag="PKJ17_06390"
     CDS             2329..2601
                     /locus_tag="PKJ17_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75637.1"
                     /translation="MARRSETAVSCCATLLVLVWGFISPLVAQAQEGPQGSAPDAGRS
                     SLIRALSRQIAFVRSFSVEYPFQFRQEAGAALTGAPAAGDRKARGE"
     gene            2598..>2802
                     /locus_tag="PKJ17_06395"
     CDS             2598..>2802
                     /locus_tag="PKJ17_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75638.1"
                     /translation="MKMGMGMEGNRRLFSLVGITALAGILLCGGGCAPTIRTVSEDVI
                     EAEAVRIAREEGREQGRREGSRLC"
BASE COUNT          638 a          734 c          865 g          565 t
ORIGIN      
        1 cggcagaccc tcgatcccgt tcaccctcat tgccgacgaa tacgggtcct atgccgttga
       61 aggttttgat accatccttg cccagggacg ctctctcggt tttggcgtca tcatatccgt
      121 tcaggagctt gcatcgctct tcaagacgtc ggaaacggac gcaaagcggc tcatcgggaa
      181 cacgaactac aagatcgtta tgaaggtgga ggatacggac acggctaaat ttctggcgga
      241 gagggcgggc gaatccttct tcatgatgcc caacatccgg aacgacagca aaatgatgga
      301 gagccttggc aactgggacg gcacctatgc ctttcagaaa ggcagccgcg tagagatgag
      361 agatctggcc gggctcaagg tcggtgaagg gtacatcttt gccggggacg aggtgagacg
      421 gttcagaacg cgctacatcc ccccggcggg taacgtgaaa caattgagat tgatgaaata
      481 tgtgaagaag acgacattgg gcaccttgca ggaaagaagg tctacagacc tgcgttgccg
      541 agatacaggc cgcagcgggt gagggtggag atgacgatcc acgaggtcag cttgtagcat
      601 gtcaggaagg ctccgatgat gatcatcttt ccacccccgg ctcctccctt cgcggcctca
      661 tttccgttgg caagccgtat gaggcccttg atgatgagga aggttcccag aacgggcatc
      721 aagggagaga ccattgtctg cgtggtgccg atgagttttg tgatcaattc ggtcatagaa
      781 gcggttcaaa aggtcaagag ttcacgggtt caagcgtcaa aagcgcgaat aaccatatgt
      841 tcgatggtgc tatttatcat atagcaggag accgatggct gttttcggaa aacaaaagaa
      901 agaggctcca gacccggacg gcggcgaggc ccttgcgata gagataggca tattccgggg
      961 attgaagctc acgtcccggg ctctgcttgt tattcccgtt gccacggtgg tcctcagcat
     1021 catcatggtg gttgcgtcga tcgtcatcgt ggttgccggg aaaggaaggg acaagatcgt
     1081 cttcatggat tccacgggaa ggccatctct cgtccgcgca gacgatcccg acaagatcca
     1141 ctggccggag cttgagatct tctgcaggga cgcgatcagg gacatcctcg gctggaccta
     1201 tgtggaggtg aggtcccaaa gcgatcttgc caaccggata agtcgcgtgt cgaagcgttt
     1261 cgatcccgcg tccttcaagg ctttttacga gccgtaccgg gacaattatc tcaagtccct
     1321 gtcggaccaa aagctcatcg tcaccgccga gtggaatgct ctcaaggact cgaagatacg
     1381 cggcaaggaa gcggtcgttc ttgcctcggt cagactcaat gccgttcgtg tggttggaga
     1441 ggaagcggcg agcgaagacc tcaagctaaa gacgtacgag atcaagctgt acaaaggagc
     1501 gcgcagtgtg gagaaccctt tcggtctgta tattgtccgt ttttctgagc ctgccggtgg
     1561 ctgaggccgc gccggacatg cctcaggaga ccccgaagca gcaggctgag gggcagatcg
     1621 ttgtccctcc cttcgatctt cccgagaagg acatcgtcga gatgaaggag aaggtgcagc
     1681 gcctcaagcc tctcatagaa aaggatcacg agctggcacg ggcgagggag cgcgatgtcg
     1741 acgaagtgcc gggtgcggtg cccaccatcc atctctcata cgggtacggc agcgtcatca
     1801 atctccctta cagtttcagc gtcgacgata tcgcgatcgg cgcaagggag aaattcaaca
     1861 tcgagacgag aggcaactcc ctcatcatct ttccagtgag agaattcaag tcgaccaaca
     1921 ttattgtttt cgaaagaacg caagcgggtg accagttgcc ccaccattat ttgcttgtcg
     1981 agaacggggc atcgggagag gcggacctca ccgtcaacgt aaggcggtca ggcatgggtg
     2041 gcgtgtccga cataacggac gcaatggtgc gcgtcatcac gacacagcgc atccccgaga
     2101 agggcacagc agaagacatt ctcctcgagg gccggtcgcc ttcactgacg aatctcagtg
     2161 actatccttt catacggatg atgaaactcc agaaaccgga gcttcatgtc ttcatgatag
     2221 ccgggaaggt gatgcccgtg ggcgatgccg attttgccct ggacctcggc aacgggacaa
     2281 gcgttgtggc atcgcggagc cgggaaataa cggtaaggag ggtgggcgat ggcaagacgt
     2341 tcagaaacag ccgttagctg ctgtgcaact cttcttgtgc tggtttgggg cttcatatcg
     2401 ccgctcgtgg cgcaggcgca ggagggaccg caaggctctg ctcccgacgc cggccgctcg
     2461 tccctcatac gcgccctttc gcggcagatc gccttcgtga ggtccttctc ggtggaatac
     2521 ccattccagt tccggcagga ggccggggca gcattgaccg gtgcgcctgc tgccggtgac
     2581 aggaaggcgc gcggggaatg aaaatgggta tgggcatgga aggaaacagg cgcctttttt
     2641 cgctcgtcgg tatcacggcc ctggcgggga tccttctctg cggaggcgga tgcgctccca
     2701 ccatcaggac agtatccgag gatgtcatcg aggcggaagc cgtgcggatc gcgcgggaag
     2761 aaggcagaga gcaaggcagg cgggaagggt cgcgcctctg ca
//
LOCUS       DAOPOA010000267         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39062_length_2802_cov_2.140153, whole genome shotgun sequence.
ACCESSION   DAOPOA010000267 DAOPOA010000000
VERSION     DAOPOA010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39062_length_2802_cov_2.140153"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PKJ17_06400"
     CDS             <1..1012
                     /locus_tag="PKJ17_06400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD75639.1"
                     /translation="SWWYDRVMDAAGACSIQLSGGEPTLREDLPEVVALGVKKGFFFI
                     QVNTNGILIAGRPGYAKVLREAGLSTVFLQFDGTEDRIYTELRGRPLLDVKMAAIEEC
                     GAAGLGVVLVPTLVAGVNTGDIGSILRLAVKLSPVVRGIHFQPLSYFGRYHVAPEPAR
                     RFTLPEVIAAITGQAGDLCDRWNFLPPGTENDHCSFHGSFLVMPDGRLKATSPSADLC
                     CRGTSDGREGLQRTVSTVARQWSPASSCCAPGIPAHRDAAAGKPLVSDEGVIDLDAFL
                     ERGRDFGFSISCMAFQDVWNIDLDRLRDCCISIMSRDGRLIPFCAYNLTSETGQTLYR
                     GR"
     gene            1002..2360
                     /locus_tag="PKJ17_06405"
     CDS             1002..2360
                     /locus_tag="PKJ17_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD75640.1"
                     /translation="MEDRPARTPLDEWIAGKIGIGRKVSPFLSRDDLDRYHLEKLRGA
                     VDYAKLNSPFYARLLTAFCGRDLTSLESVASLPFTTAQDIKDSPLSFLCVSQDEIARV
                     VTLTTSGTTGASKRFFFTAEDLELTIDFFHHGMSTLVSPGQRVLILLPGEKRDSVGDL
                     LKRGLARMDVEGIVHGPVRDVEKALSDVVRFGIDCLVGIPTQALWMARKGSGSIPAGR
                     VKSVLLSTDYVPRAIIEELGRLWGCPVFSHYGMTETGLGFAVECAAVSGHHIREADLY
                     VEIVDPVTGLPCADGRQGEIVFTTLTRKGMPLIRYRTGDISHFMAERCACGTVLKRLG
                     RIAGRFEGRVRVGKGHALAIPDLDEALFPIPGLINYSVEIVRGGGKDLLLLSLHSIGT
                     VDDGDIANAARGAAMTVPDIHEAVTEGSLEVPLPTFTPTNPFTTGVGKRRIVVKETGN
                     GL"
     gene            complement(2435..>2802)
                     /locus_tag="PKJ17_06410"
     CDS             complement(2435..>2802)
                     /locus_tag="PKJ17_06410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOD75641.1"
                     /translation="FLHENAHIKYDHAAKNALLPDATSAAFTIAGLFLPGVGYGNLIV
                     NPLVTSGYSRSQELEADKDVIDQCHLLRLTPDHYVSALTKLKQYAASKGESSDSTGLF
                     DTHPNLQYRIDAINKVTGR"
BASE COUNT          506 a          843 c          892 g          561 t
ORIGIN      
        1 cagctggtgg tatgatcgcg ttatggatgc ggccggcgcg tgcagcatac agctgtccgg
       61 cggggagccc acgctcaggg aggacctgcc ggaagtggtg gccctggggg tgaagaaggg
      121 gtttttcttt atccaggtga atacgaacgg gatcctcatc gccggcaggc ccggttacgc
      181 gaaggtactg cgcgaagcgg gcctttccac cgttttcctc cagtttgacg gaactgagga
      241 ccggatatac acggaactca ggggccgccc gcttcttgac gtgaagatgg ccgccatcga
      301 agagtgtggt gcggccggac tgggagtggt cctcgttccc acccttgtgg cgggggtgaa
      361 tacgggcgac atcggatcca tcctccgcct tgccgtgaag ctgagccccg tcgtgagagg
      421 tatccatttt caacctctca gctatttcgg gcgctatcac gtcgcacccg aaccggcaag
      481 aaggttcact ctccccgagg tcatcgcggc gatcaccggg caggcgggcg atctttgcga
      541 ccgatggaat ttcctgccgc cgggcaccga gaacgaccat tgctcctttc atggcagttt
      601 ccttgtgatg cccgacggcc gtctgaaggc gacgtccccg tcggccgacc tgtgctgccg
      661 ggggacgagt gacggcaggg aagggctcca gcggaccgtt tccaccgtgg ccaggcaatg
      721 gtcacccgcc tcatcctgct gtgcgcccgg cattccggca cacagggatg ccgccgccgg
      781 caaacccctc gtttccgacg agggtgtcat cgacctcgac gcgttcctgg agaggggaag
      841 ggacttcggg ttctccattt cctgcatggc ctttcaggac gtctggaaca tcgacctcga
      901 ccggctgcgg gactgctgca tctccataat gtcacgcgac ggccgtctca ttcccttctg
      961 cgcgtataat ctcacgagtg aaacggggca gacgctttac cgtggaagat aggccagcaa
     1021 gaacacccct cgatgaatgg atcgccggga agatcggaat cggcaggaag gtatcgccct
     1081 tcctctcccg ggatgacctt gatcggtatc accttgagaa gctccgcggc gcggtggact
     1141 atgcgaagct gaacagcccc ttctacgcaa ggctcctcac ggcgttttgc gggagggacc
     1201 tcacgtctct ggaaagtgtc gcttccctgc ctttcacgac ggcacaggac ataaaggact
     1261 cccccttaag cttcctctgt gtctctcagg acgagatagc gcgcgtcgtt accctcacca
     1321 cctcgggcac gacaggcgcg tcgaagcggt tctttttcac cgcggaggac ctggaactca
     1381 ccatcgactt tttccatcac ggcatgtcca ccctcgtatc ccccgggcag cgggtgctca
     1441 tactcctccc gggggaaaag agggacagcg tgggtgacct tctgaaaagg gggcttgcca
     1501 ggatggatgt ggagggaatc gttcacggcc cggtgcgcga tgtcgaaaag gcgctgtcgg
     1561 atgtcgtgag gttcggcatc gactgcctcg tcgggatacc cacgcaggcg ctctggatgg
     1621 cacggaaggg gagcgggagt atcccggccg gccgggtgaa gagcgtactc ctgtcgacgg
     1681 actacgtccc gagggccatc atcgaggaac tcgggaggct gtggggttgt cccgtcttct
     1741 cccattacgg gatgacggag acgggcctcg gcttcgccgt cgaatgcgcg gccgtaagcg
     1801 gtcaccacat ccgggaagcg gatctctacg tggagatcgt ggaccccgtc acgggcctgc
     1861 cttgcgccga cgggcggcag ggggagatcg tcttcaccac acttacccgg aagggaatgc
     1921 cccttatccg gtaccgcacc ggggacatct ctcatttcat ggccgagcgc tgcgcctgcg
     1981 gcaccgtgct caaacgcctc ggcaggatcg cgggcaggtt cgagggcagg gtgcgcgtcg
     2041 gcaagggcca tgcccttgcc atccctgacc tcgacgaggc cctgttcccc attccgggat
     2101 tgatcaacta ctccgttgag atcgtccgcg gcggtgggaa agacctgctc ctcctgtctc
     2161 tccacagcat cggcaccgtc gatgacggcg acatcgcgaa tgcggcccgc ggggccgcga
     2221 tgacggtgcc cgacattcac gaagcggtta cggaaggttc cctcgaagtc cccctcccca
     2281 ccttcacccc gacaaacccc ttcacgacgg gggtggggaa aaggaggata gtggtgaaag
     2341 agacgggtaa tgggttatag gtcataggtt ataggaagac agcatctttc ccgtaccgcc
     2401 catcgcccat gacccatcac ccatgaccca tcacctatct tcccgtcacc ttgttgatcg
     2461 cgtctatcct gtactgcagg ttggggtggg tgtcgaagag gcctgtcgag tcggagcttt
     2521 ctcccttcga tgcggcgtat tgcttgagct ttgtgagggc tgaaacgtaa tggtccggtg
     2581 tgagcctgag gaggtggcac tggtcgatga cgtccttgtc ggcctccagt tcctgtgacc
     2641 tgctgtaccc gctcgtgacg agaggattga cgatgaggtt cccgtaaccc acgccgggga
     2701 ggaagagccc ggctatggtg aacgccgctg atgtggcgtc ggggaggagg gcgttcttcg
     2761 ccgcgtggtc gtacttgatg tgggcgttct cgtgaagaaa ga
//
LOCUS       DAOPOA010000268         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39084_length_2800_cov_4.517668, whole genome shotgun sequence.
ACCESSION   DAOPOA010000268 DAOPOA010000000
VERSION     DAOPOA010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39084_length_2800_cov_4.517668"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PKJ17_06415"
     CDS             complement(<1..241)
                     /locus_tag="PKJ17_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75642.1"
                     /translation="MKNAALRTALAVVTIFALVICSFGITSAQEKKKGPALQQLEQAA
                     GKKIEDVKVPAVPEPTPVKTKEVQKSTSTTSSFDVN"
     gene            complement(275..574)
                     /locus_tag="PKJ17_06420"
     CDS             complement(275..574)
                     /locus_tag="PKJ17_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75643.1"
                     /translation="MKKILSAAAILTLLASTGLCYGADTVKTTPQEYFKYAGTAADPS
                     YQDYVATSSRKTSGNTGNNAGSYTAQRERTQERMRNSPRYTVPQINHKGTYGQAK"
     gene            complement(878..2236)
                     /locus_tag="PKJ17_06425"
     CDS             complement(878..2236)
                     /locus_tag="PKJ17_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOD75644.1"
                     /translation="MKTTDRPTGVIFFPAFDWAISPTHPEREERLLYTRDQIFEEGIM
                     DLPEIREYHPRLASAKDVSRVHFCVPDVESRVTEAHMVSAGCAMVMADAFMQKEVKNA
                     FAIIRPPGHHAMTVVHGNRGFCDINNEAVMVEYLRRTYGVRRIAIVDTDVHHGDGTQE
                     IFWHDPDVLFVSFHQDGRTLFPGSGFTNELGGPLAYGTTINIPMAPRTTDEGIHYIMD
                     NLVLPILDEFKPELVINSAGQDNHYSDPLANMSFTAKGYAILNQKLRPDIAVLEGGYS
                     VETALPYINMAIIMAMAGIDFSNLREPDYNPSRFKESAKNMDYIRALVTHQLATFRDR
                     ERVVEANRKEGRDFYGSSRSTFYDTEYLQEEQRLDLRMCPDCCGYRRISSSCAHHSGR
                     SYSVFCVALPAACCPDCAARARQDYEDMKKKPGYGYLYLQDRIDDEYKTFETKTGREN
                     TL"
     gene            complement(2237..>2800)
                     /locus_tag="PKJ17_06430"
     CDS             complement(2237..>2800)
                     /locus_tag="PKJ17_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75645.1"
                     /translation="ELCQKVADEVVERLEWNIEEMFGLWEDEPAYKVWEVVHRRKFEL
                     HRVIGIGAASPAIVPRLAAKMGVEHFLHRYSPVANALGAAVARPTLAVEVHVDTARRT
                     YTGSPGGLRGTVEKRSFQLEDARELALNCLREASRERGLADYADEAHVYLEEQFNMIR
                     GWEQEGKLFDVGIQIAPGFIHEYKGVA"
BASE COUNT          502 a          833 c          806 g          659 t
ORIGIN      
        1 tgttcacgtc gaaggaggat gttgtggatg tggatttctg cacctccttc gttttcacgg
       61 gggtgggctc aggcactgcg ggcactttca cgtcctcgat cttcttcccc gccgcctgct
      121 cgagctgctg gagtgccggg cctttcttct tctcctgcgc cgaggtaatg ccgaaactgc
      181 agataacgag tgcaaagatc gtcacaactg ccagggcggt tctcaatgct gcgttcttca
      241 tggtctttct cctttatcct tatagtgtta cgttctattt tgcctgtccg taggttccct
      301 tgtggttgat ctgcggcacg gtgtaccgcg gggaattcct catgcgctcc tgcgttctct
      361 cccgctgcgc cgtgtagctg ccggcattgt tgccagtgtt gccgcttgtc tttcgggagc
      421 tcgtagctac gtagtcctga taggaggggt cggcagctgt ccccgcatat ttgaagtact
      481 cctgcggcgt cgtcttgacg gtatccgcac catagcacag accggtagaa gccagaaggg
      541 tcagaatagc tgctgcgctc aggatctttt tcattttcgg tctccttatc atttttcaag
      601 gtgagacaag gggaaggaaa aaaagttcca gaaaaaactg ttgcgggttc cgggttgcga
      661 gtcaagaacc ttaaaggccg ttcccgacag accgttggca aagtcccttt ctgcgtcatt
      721 ccggcaaagg ccggcagacg gaccggacgg gaagcgattc tatcaacaac ctgtcaggtc
      781 tctgacgtga aacccgcaac gacctttgct caatgaaact tgaagttgct ttcaaggctt
      841 ttaacccgca actcggaacg cgcaacccgc aacactttca taacgtgttt tcccgtccgg
      901 tttttgtttc gaaggtcttg tattcgtcgt ctatcctgtc ctggaggtag aggtagccgt
      961 aaccaggttt cttcttcatg tcctcgtaat cctgccgcgc ccgtgccgcg cagtcgggac
     1021 agcaggcggc agggagcgcg acgcagaaga cggaatagct gcggccgctg tggtgggcgc
     1081 atgatgacga gatacggcgg tagccgcagc agtcggggca catcctgagg tcgagacgct
     1141 gctcctcctg gaggtactcc gtatcgtaaa aggtgctgcg ggaactgccg tagaaatcac
     1201 ggccttcttt tcggttcgcc tccacaaccc tttccctgtc ccggaaagtg gcaagctggt
     1261 gcgtgacgag ggccctgatg tagtccatgt tcttcgccga ttccttgaag cgggacgggt
     1321 tgtaatcggg ctcgcgcagg ttggagaagt cgatccccgc catggccatt attatggcca
     1381 tattgatgta aggcagggct gtctcgacgg agtacccccc ttccagcacc gctatgtcgg
     1441 gcctcagctt ctggttgagg atggcatacc cctttgccgt gaagctcatg ttcgccaggg
     1501 gatcgctgta gtgattgtcc tgtccggccg agtttatcac cagctccggc ttgaactcgt
     1561 cgagtatggg caggacaagg ttgtccatga tataatggat gccctcgtcc gtcgtcctcg
     1621 gcgccatagg tatgttgatg gtggtgccgt acgccagcgg ccctccgagc tcgttcgtga
     1681 aacccgaccc cggaaaaaga gtacgcccgt cctgatggaa ggatacgaag aggacgtcgg
     1741 gatcgtgcca gaagatctcc tgcgtcccgt ccccgtggtg aacgtcggtg tcgacgatgg
     1801 cgatgcgcct tacgccgtat gtccgtcgca ggtactccac catgacggcc tcgttgttga
     1861 tgtcgcagaa accccgattt ccgtgaacga cggtcatggc atggtggccg ggcgggcgga
     1921 tgatggcgaa ggcgttcttc acttccttct gcatgaaggc atcggccatc accatggcgc
     1981 atcccgccga taccatgtgc gcctcggtca cgcggctctc gacgtcgggg acgcagaagt
     2041 ggacgcgcga gacgtccttt gcagacgcga gccggggatg gtattccctt atctcgggca
     2101 ggtccatgat gccttcctcg aagatctggt cgcgcgtgta gagaagcctt tcctccctct
     2161 cgggatgggt ggggcttatg gcccagtcaa aggcgggaaa aaatatgacc cccgtgggtc
     2221 tgtccgtcgt tttcatctat gccaccccct tgtattcatg aatgaatccg ggggcgatct
     2281 gtatccccac gtcaaagagc ttgccttctt gctcccagcc gcggatcata ttgaactgct
     2341 cctccagata cacatgcgcc tcgtccgcat aatcggcaag cccccgttcg cggctcgcct
     2401 ccctgagaca attcagcgcc agttcccttg cgtcttcgag ctgaaagctg cgtttctcga
     2461 cggtacccct caaccctccc ggcgaacccg tgtaggtccg ccgtgccgtg tcgacgtgga
     2521 cctccaccgc gagtgtcggc cgtgccacgg ccgcgccgag ggcgttggcg acgggcgagt
     2581 accggtgcag gaagtgctcg acgcccatct tcgccgccag ccgggggacg atggccgggg
     2641 acgccgcgcc tatcccgatc acccggtgga gctcgaactt cctcctgtgt accacctccc
     2701 agaccttgta ggcaggctcg tcctcccaga ggccgaacat ctcctcgatg ttccattcca
     2761 gcctctcgac cacctcatca gcgaccttct ggcagagctc
//
LOCUS       DAOPOA010000269         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39147_length_2797_cov_2.637856, whole genome shotgun sequence.
ACCESSION   DAOPOA010000269 DAOPOA010000000
VERSION     DAOPOA010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39147_length_2797_cov_2.637856"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKJ17_06435"
     CDS             <1..342
                     /locus_tag="PKJ17_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75646.1"
                     /translation="ENGEIGKAGRYEGTVTRLQTVRCSSDPGDKRYCKRIYLAVDPGV
                     PLTSRMHLYTEITRKGKTVYHGPDEDKSLVEQINSMDRLVNKILKPNTRASVSVSCGD
                     EGCLVDRIDLR"
     gene            complement(372..776)
                     /locus_tag="PKJ17_06440"
     CDS             complement(372..776)
                     /locus_tag="PKJ17_06440"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOD75647.1"
                     /translation="MTTAVEYLKVNPVFHIATVDGTKARVRPFGFSMKRNNALYFCTN
                     KTKDVYKQMSQNPEVEISDMGSDGTWLRIRGRIAFDDSREAKAQAFAESENLLKIYPK
                     GADDETFVTFYFVEAVATLFSFTAAPKSIPLV"
     gene            complement(948..2120)
                     /locus_tag="PKJ17_06445"
     CDS             complement(948..2120)
                     /locus_tag="PKJ17_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOD75648.1"
                     /translation="MQVNAEKVRVPESQPLMGAKRMLYLDNLRLSFTFLVILHHVCLT
                     YAANSGWYFYQHLDDPFTNIILNILMGVGRTWVLASFFLISGYFTPGSLDRKGTWWFI
                     KDRFIRIGIPLGIFALIIRPTMVYLLKRDTLSLQYSYLENIFLLKNAAPGPAWFLEVL
                     LVFSLAYAAWRVLTRSNRQRDREARPFPGSGAIILFILVLAAFTFVMRIYLPSEKQIF
                     HLRLGNYAEYVAFFFGGIVAYRNRWLEKLTDTVGKQWTVITAVAVCVYILFIVQAWGT
                     GENLSFLRGGFSLKTLIATYVGTFIAVGSSICLVYLFRTYLNGQPGVIGPMTQDAYAV
                     FIFHSPVIVAVTYYMQDTAVLHPFIKFLSAFVVGTVLCFLLCHYLVRKIPYAKRVL"
     gene            2245..>2797
                     /locus_tag="PKJ17_06450"
     CDS             2245..>2797
                     /locus_tag="PKJ17_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD75649.1"
                     /translation="MTRSARYASLLIVISVAFSSFLVRLNNYMVNISLPTITRSFGVT
                     SSDASRIISSYLLIITTTLLLFGKLGDRIGLKRVFISGYVIFTVGSLMCGLSHDIDML
                     IGSRFIQGIGSAMLLAVSFAIISHFLPEGNTGWAFGITSTASALGVATGAPVGGIITG
                     YLSWNWAFFINVPVGIVAVIVAMK"
BASE COUNT          621 a          725 c          847 g          604 t
ORIGIN      
        1 gagaacgggg agatcggaaa agcgggccga tacgagggca ccgttacgag gttgcagacg
       61 gtccgctgtt cctcggaccc cggcgacaag agatactgta agaggatcta tcttgctgtt
      121 gatcccggag tgccgctgac atccaggatg catctttaca ccgagatcac acggaagggg
      181 aagactgttt atcacggccc cgatgaggat aagagcctcg tggagcagat caacagcatg
      241 gacagactgg tgaacaagat cctcaaaccc aacaccaggg catcagtgtc ggtatcctgt
      301 ggcgatgagg gatgcctggt cgaccgcatc gacctgcgct gaaacccttg aaccgcctcc
      361 gcttttgcct gttagaccag aggtatgctc ttaggggcag cagtgaagga gaagagcgtt
      421 gccacggcct cgacgaaata gaacgtcacg aatgtctcgt cgtcggcacc cttcgggtag
      481 atcttgagaa ggttctcgga ttcggcgaag gcctgggcct ttgcttcgcg ggagtcgtca
      541 aaggcaatgc ggccccgtat cctcagccat gtgccgtcgc ttcccatatc ggagatctcg
      601 acctcagggt tttgggacat ctgcttgtac acgtccttgg tcttgttcgt gcagaagtac
      661 agggcgttgt tccttttcat gctgaaaccg aagggccgga cgcgggcctt cgtgccgtca
      721 acggtggcga tgtgaaaaac agggttgacc ttcaggtact caacggcagt agtcatgtaa
      781 acctccgaaa tgtatttgct tgccggcgcc tccgggacac tgcccgacgg gatcttgtac
      841 ataccgggtc gctttctcta gcagcgtgtg atagaaggcg gcattgaatt ctcctgctat
      901 cttcgagcgt tgtcaagaca attgtccccg aagaaagccg tggtccgtca caacactctc
      961 tttgcgtaag ggatctttct gacgagatag tggcagagaa ggaaacaaag caccgttccg
     1021 acgacgaagg ccgaaaggaa cttgatgaag ggatgaagga cggccgtgtc ctgcatgtaa
     1081 taggtgacgg cgacgataac gggggagtgg aagatgaaga cggcgtaggc gtcctgcgtc
     1141 atcggtccta tgacgcccgg ttggccgttg aggtatgtcc tgaagagata gacgaggcag
     1201 atggagctgc ccacggcgat gaaggtgccc acgtatgtcg cgatgagggt cttgagggaa
     1261 aagcctcctc tcagaaagga gaggttttca cccgtgcccc aggcctgaac gatgaagagg
     1321 atatagacac acacggccac cgcggtgatg accgtccatt gcttcccgac ggtgtccgtc
     1381 agtttctcga gccatcggtt ccggtatgcc acgatgcccc cgaagaagaa ggccacatat
     1441 tccgcgtagt ttccgagacg gagatggaag atctgctttt ccgagggaag gtagatgcgc
     1501 attacgaatg tgaaggccgc gaggacgagg atgaacagga tgatagcgcc gctgccgggg
     1561 aagggccggg cctcgcggtc cctctgtcgg ttcgaacggg ttaggaccct ccaggccgcg
     1621 taggcgaggg agaagacgag gagcacctcg aggaaccatg cgggtcccgg agcggcgttc
     1681 ttgagaagga agatgttctc caggtaggaa tactgcagag agagggtgtc ccgtttcaac
     1741 aggtagacca tggtggggcg gatgatgagc gcgaatatgc cgagaggtat gccgatcctg
     1801 atgaaacggt ccttaatgaa ccaccaggta cccttcctgt ccagcgagcc gggcgtgaag
     1861 tatcccgaga tgaggaagaa ggatgccagg acccaggtcc tgccgacacc catgaggatg
     1921 ttgagaatga tgttggtaaa agggtcatcg agatgctgat agaagtacca accgctgttg
     1981 gcggcgtagg tcaggcatac gtggtgaagg atcacgagga aggtgaagga gagtctgagg
     2041 ttatcgaggt acagcatccg tttcgcgccc atcagaggct gcgactctgg aacccgtacc
     2101 ttttccgcgt tgacttgcat acagagttat tcattgtgcc acatcgtgtt ttcgactgca
     2161 acgattatat ggggagaggg tcggtcctgt ctttccaaga aaaacattgc ggaaggtcgt
     2221 gaaagagtgt aagtaatttc attcatgacg cggtcggcca gatacgcctc actcctcatt
     2281 gtcatcagcg ttgccttcag ctcctttctg gtgcgtctca acaattatat ggtcaatatc
     2341 tccctgccca cgatcacccg ttccttcggc gtgacctcga gcgacgcgtc ccggataata
     2401 tcctcctatc ttctcatcat cacgacgacg ctcctgcttt tcgggaaact cggcgacagg
     2461 atagggctca aacgggtctt catctcgggg tatgttatct tcaccgtggg gtcgctcatg
     2521 tgcggactgt cccacgatat cgacatgctc atcggctcgc ggttcatcca gggcataggc
     2581 agcgccatgc ttctcgccgt gagttttgcc atcatctccc attttctccc ggaggggaac
     2641 acgggctggg ccttcggcat cacctccacc gcctcggcgc ttggcgtggc gacaggcgcc
     2701 ccggtgggcg ggataatcac cggctatctc tcatggaact gggccttctt catcaacgtg
     2761 cctgtcggca tcgtcgcggt gatcgtggca atgaaat
//
LOCUS       DAOPOA010000270         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39230_length_2793_cov_3.263696, whole genome shotgun sequence.
ACCESSION   DAOPOA010000270 DAOPOA010000000
VERSION     DAOPOA010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39230_length_2793_cov_3.263696"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PKJ17_06455"
     CDS             <1..870
                     /locus_tag="PKJ17_06455"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOD75650.1"
                     /translation="YTVDKEEYFRIAAGRTTLVIGRVLVASRITGDSERISFALLHLG
                     GHAFNGGVREYLGEKDFQTMRTDMTAAFEKADFAEVFRLMDHHFGMHNYSFTSLFRDR
                     QHAVMNLLLKDTYDKYEVVYRDLFEGERILMNFFREAGMMVPNVFRAAAEFILNLDLR
                     KAFSQETLDANHIRGLVKEVEKWGLGYDTVQIETIIRKRLEDQMYLLQANPSNISLIE
                     AVKASTVLSGLLPVQVNLWEAQNIYYSMARSVYRGMVKEASGNRPEAVKWITAFIELG
                     EKLGFDTGSIPGR"
     gene            complement(1048..1254)
                     /locus_tag="PKJ17_06460"
     CDS             complement(1048..1254)
                     /locus_tag="PKJ17_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75651.1"
                     /translation="MAKRNPNSFLKRQKEIKRQQKAQEKMLRRQGHKKQEPEEEINEA
                     TAADEVETEGEDTPAGEKEEEETV"
     gene            complement(1604..2065)
                     /locus_tag="PKJ17_06465"
     CDS             complement(1604..2065)
                     /locus_tag="PKJ17_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOD75652.1"
                     /translation="MAHILVTFHSQTGNTEKMARAVAAGVAQTRNARANLKAALETTA
                     EDIRDCDAVVFCSPEYFGYMSGAMKDLFDRTYEELKDDPRSLKKPYCIVISAGNDGSG
                     AVRHIERICKGYRFKKVQNPMICKGPVTPDMLALCEELGETIAEGINARIF"
     gene            complement(2095..2361)
                     /locus_tag="PKJ17_06470"
     CDS             complement(2095..2361)
                     /locus_tag="PKJ17_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75653.1"
                     /translation="MRCRVPVLVVMTMALLFSMAEGVSFAQSKVSCTDAVCGPGYRAY
                     LQPNGSCMCILADCESIEGCPEPPRVPEDCLSIEGCNPPQTIEP"
     gene            complement(2569..>2793)
                     /locus_tag="PKJ17_06475"
     CDS             complement(2569..>2793)
                     /locus_tag="PKJ17_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75654.1"
                     /translation="RYHLTTIMITHSMQQALALGSRTILMHKGQVIEEIPGKDKQYLT
                     TSDLLDRFADLRKQEKLTADMIEEMRREYL"
BASE COUNT          560 a          781 c          781 g          671 t
ORIGIN      
        1 tacaccgtgg acaaggagga gtatttcagg atagcggcgg gcaggacgac gcttgtcatc
       61 ggcagggtcc tcgtcgcctc ccgcatcacg ggtgattccg agcggatcag tttcgcgctc
      121 cttcacctgg gaggacatgc cttcaacgga ggggtgcggg aatacctcgg ggaaaaggat
      181 tttcagacaa tgagaacgga tatgaccgcc gccttcgaaa aggcagactt tgccgaggtg
      241 ttccgcttga tggaccatca tttcggaatg cacaattact cgttcaccag ccttttcagg
      301 gaccggcagc acgcggtcat gaacctcctt ctgaaggata cgtatgacaa gtacgaggtg
      361 gtctacaggg atctttttga aggcgaacgg atcctcatga acttctttcg cgaggccggc
      421 atgatggttc ccaatgtttt cagggccgcc gccgagttca ttctcaatct cgatctcaga
      481 aaggcctttt cccaggagac cctcgacgcg aaccatattc gcggacttgt gaaggaggtc
      541 gaaaaatggg gtctcggtta tgatacggtc cagatagaga cgatcatcag gaagaggttg
      601 gaggatcaga tgtacctcct gcaggcgaac ccgtcgaaca tctcgctcat agaggctgtg
      661 aaggcgagca ccgtgctctc cggtctattg cccgtccagg tgaatctctg ggaggcccag
      721 aacatctact actccatggc caggtccgtt tatcgcggca tggtgaagga ggcgtccggg
      781 aacagaccgg aagcagtcaa atggataaca gccttcattg aactcgggga gaagctcggt
      841 ttcgacaccg ggtccatccc ggggcggtag gggggattcc ggaaagggca cgtccatccg
      901 gtcccggagc gcatccacct tcaacaggcg gcgccctttt tccccacaat ggtcttgttg
      961 gtgattcgtt agctggtcgt tgtttgcgcg gggcgtcaat ggaacccgcc cctgttcatg
     1021 acaccgctga aacgtggatt cccggctcta gaccgtctct tcctcttctt tttccccggc
     1081 gggagtgtct tcgccttcgg tttccacctc gtcggcggct gtcgcttcgt tgatctcttc
     1141 ttcgggctcc tgtttcttgt ggccctgcct tctcaacatc ttttcctgtg ctttctgttg
     1201 acgcttgatc tccttctgcc ttttcaagaa actgttcgga ttgcgtttcg ccattcaggc
     1261 tcctttcttc ttcccggtgt gcctctcggg ctctcaatgc aatgtctccg gtgacctgcc
     1321 cgggaggaat gggtcgtgta aagatgcaga tatggattat gacttgattt gaagggtttg
     1381 tcaagccaca aagccggaac ggccgccgga acggccgttc aagggataaa agacagttca
     1441 agagttcaag agttcaagag ttcaagagtg aaagacaaaa aacaacggac taccctggtg
     1501 tcctggccgg caattgtgtg ctaagccact gacctcgaac ggttgtcaac ataatcactg
     1561 accagggcat gctatggtcg gtcacttgag cccttgaacg gtcttagaat atccttgcgt
     1621 tgatgccctc ggcgatggtt tcgccgagtt cttcgcacag ggccagcatg tccggcgtga
     1681 cggggccctt gcatatcatg ggattttgca ctttcttgaa gcggtagccc ttgcagatac
     1741 gttctatatg cctgaccgcg ccgctgccgt cattgcccgc gctgatgaca atgcagtagg
     1801 gtttcttcag cgaccgcggg tcgtctttca gttcttcgta agtcctgtca aagaggtcct
     1861 tcatcgcgcc cgacatgtag ccgaaatatt cgggggagca gaagacgacg gcgtcgcagt
     1921 cccggatatc ttcggcggtg gtttccaggg ccgccttgag attcgcccgc gcgttccggg
     1981 tttgtgctac gccggcggcg acggcccggg ccattttctc cgtgttgcct gtttgcgagt
     2041 gaaatgtgac aagtatatgg gccatttcag gacctgccgg gaggggtccg cgccctaggg
     2101 ctcgatcgtc tgcggcgggt tgcagccctc gatgctcaga caatcttcgg ggactctggg
     2161 aggctccggg cagccctcga tgctttcgca atcagccaga atacacatgc acgacccgtt
     2221 cggttgcaga tatgccctgt aaccggggcc gcacacggca tcggtgcagg acacctttga
     2281 ctgggcgaac gagacaccct ctgccatcga gaataagagg gccatcgtca tcacgacaag
     2341 aacaggaacc cgacaacgca tacccacacc tcctcagcgc ggccttactg caattgatat
     2401 tatcctaatg cctgcacggc aaagagtaaa gcagaaaaac cgttcacgcc atcccaatgg
     2461 tccacggtcc cccctcaagc tactcaaacg actcacgccg tctttctttc tgtcttttgc
     2521 ctttactctc aacaggctgt caagccattc aagctgttca agccgcctct acaaatattc
     2581 cctcctcatc tcctctatca tatcggcggt gagcttctcc tgctttctca ggtcagcgaa
     2641 gcggtcgagg aggtctgagg tggtgaggta ttgcttgtcc ttgcccggga tctcctcgat
     2701 gacctgaccc ttgtgcatga ggatggtccg cgagcccagc gcgagggcct gctgcatgct
     2761 gtgggtgatc atgatggtcg taaggtggta gcg
//
LOCUS       DAOPOA010000271         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39241_length_2792_cov_3.937157, whole genome shotgun sequence.
ACCESSION   DAOPOA010000271 DAOPOA010000000
VERSION     DAOPOA010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39241_length_2792_cov_3.937157"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..605
                     /gene="hslV"
                     /locus_tag="PKJ17_06480"
     CDS             81..605
                     /gene="hslV"
                     /locus_tag="PKJ17_06480"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOD75655.1"
                     /translation="MEATTVLCVRHNGGIAIGGDGQVTMNATVMKHTAKKVRKLYNDK
                     CLAGFAGATADAFTLFERFEKKLEQFNGNITRAAVELAKDWRTDRLLRRLEALLIVAD
                     RDHTLILSGNGDVIEPDDGIAAIGSGGPYAQAAAKALVKHAPLSAVEIVREAMAIASD
                     ICIYTNEKVTIEEL"
     gene            605..1936
                     /gene="hslU"
                     /locus_tag="PKJ17_06485"
     CDS             605..1936
                     /gene="hslU"
                     /locus_tag="PKJ17_06485"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOD75656.1"
                     /translation="MKQLTPKEIMEELDRFIIGQNKAKKAVSVALRNRWRRQMIPADL
                     RDEVAPKNIIMIGPTGVGKTEIARRLAKLANAPFLKIEATKFTEVGYVGRDVESMIRD
                     LTELSVNMLKKEEQERVKEKATIMAEERILDLLIPKRRASSAGEEEESPDQSREKMRM
                     RFRAGSLNDRPVELEVPDRALPIIEIFSSQGMEEMDMQLRDMFGNMLPQKTKRRKMKV
                     GEAYEHLIQEESRKLIDMDRVTSEAVDRVEQSGIIFLDEIDKIASRDHGHGPDVSREG
                     VQRDILPIVEGTTVTTKYGMVKTDHILFIAAGAFHMSKPSDLIPELQGRFPIRVELDA
                     LTKDDFYRIITEPDNALIKQYRALLKTEDVELEFHEEAIREITDIAQRINEMTENIGA
                     RRLYTVMEKLLDDISFTAPDLKGQKVEVTKSYVREKLGEFLDKEDLSRYIL"
     gene            1950..2792
                     /gene="argB"
                     /locus_tag="PKJ17_06490"
     CDS             1950..2792
                     /gene="argB"
                     /locus_tag="PKJ17_06490"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOD75657.1"
                     /translation="MMSEKIQTLLEALPYISKFAGSIFVIKYGGAAMEEESLKKEFAK
                     DVALLKYVGINPVIVHGGGPMIGRLLKDLQIPTRFVNGLRVTDEKTLEVAEMVLSGLV
                     NKEIVKNINDMGGKAIGLSGKDGRLLMAKQVEDREVGLVGEITSVDVAIIKDISRHGY
                     IPVIAPIADGVDGKAYNINADTAAGSIAAALSAEKFILLTDVQGVMGADGKLISVLKR
                     SEIEKLIDDNVVTGGMIPKVTCCTEALKGGVREAHVVDGRTPHAILLEVFTDSGIGTE
                     ITGD"
BASE COUNT          685 a          757 c          890 g          460 t
ORIGIN      
        1 ccacgacgca aaaatatact cacgtctcca tggacaagct catggagatt tacgacaaat
       61 cacatcccag aaggtaaaga atggaagcca cgactgtttt gtgcgtcaga cacaatgggg
      121 gaattgctat tggaggggac gggcaggtca cgatgaacgc cacggtgatg aaacacacgg
      181 cgaagaaggt ccggaaactc tataacgaca agtgcctggc gggattcgcg ggggcgacgg
      241 cggatgcctt caccctcttc gagcgtttcg agaagaaatt ggagcagttc aacgggaaca
      301 tcaccagggc ggccgtggaa cttgccaagg actggaggac ggataggctt ttaaggaggc
      361 tcgaggccct ccttatcgtt gccgaccgcg accacacgct catcctttcc ggcaacggcg
      421 atgtgatcga gcctgacgac gggatagcgg cgatcggttc cggcggtccc tacgcgcagg
      481 cggcggcaaa ggcgctggtg aagcacgcgc ccctttctgc cgtcgagata gtcagggagg
      541 ccatggcgat cgcctcggac atatgcattt acacgaatga aaaggtaacg atagaggaac
      601 tttgatgaaa cagttgacgc cgaaggagat catggaggag ctggaccggt tcatcatcgg
      661 ccagaacaag gcgaagaagg ccgtgtcggt ggcgctgcgc aaccgctggc ggcgccagat
      721 gattccggcg gacctgcgcg acgaggtggc gcccaagaac atcatcatga taggccccac
      781 gggcgtgggc aagacggaga tcgcccgcag actggccaaa ctggccaacg caccgttctt
      841 gaagatagaa gcgacgaaat tcaccgaggt ggggtatgtc ggcagggacg tggaatcgat
      901 gatcagggac ctcaccgagc tctccgtgaa catgctcaag aaagaggaac aggaacgggt
      961 caaggaaaaa gccacgatca tggcggagga gaggatcctc gacctgctca tcccgaagag
     1021 acgggcaagt tcggcgggcg aggaggagga aagccccgac cagtcgcggg agaagatgcg
     1081 catgcgcttc cgggcgggca gcctcaacga tcgtcccgtg gagctcgagg tccccgaccg
     1141 ggcccttccc atcatagaga tattctcttc ccaggggatg gaagagatgg atatgcagct
     1201 tcgggacatg ttcggcaaca tgcttcccca gaaaacgaag cggcgcaaga tgaaggtcgg
     1261 cgaggcctac gagcacctca tccaggaaga gtcccgcaag ctcatcgaca tggaccgggt
     1321 cacctctgag gccgtcgacc gcgtggagca atcggggatc atcttcctcg acgagatcga
     1381 caagatagcg agccgcgacc acggccacgg gccggacgtg tcgcgcgagg gcgtccagag
     1441 ggacatcctc cccatcgtgg agggaacgac ggtcaccacc aagtacggca tggtgaagac
     1501 ggaccacata ctcttcatcg ccgcgggggc ctttcacatg tcgaagccct cggacctcat
     1561 ccccgagctg cagggccgtt tccccatccg ggtggagctc gacgccctga cgaaggacga
     1621 tttttaccgg atcatcacgg agcccgacaa cgccctcatc aagcagtacc gggcgctcct
     1681 caagacggag gacgtggaac tggagtttca cgaggaggcc atccgggaga tcacggacat
     1741 agcccagcgg ataaacgaga tgacggagaa cataggcgcc cggaggctct acaccgtgat
     1801 ggagaagctc cttgacgaca tctcctttac ggcgcccgac ctgaagggcc agaaggtgga
     1861 agtgacgaag tcctatgtgc gggagaagct gggagagttc ctggacaaag aggatctgag
     1921 caggtacata ctctaacgat cggggtacca tgatgagcga aaagatacag acgcttctgg
     1981 aagcacttcc ctatatcagc aagtttgccg ggtccatatt cgtcataaaa tatggcgggg
     2041 cggccatgga ggaggaatcc ctgaagaagg agtttgcgaa ggacgtggcg ctcctcaagt
     2101 atgtcgggat caatcccgtc atcgtccacg gcgggggccc catgatcgga aggctgctca
     2161 aggacctcca gatacccacg cgctttgtca acggcttgag ggtcaccgac gagaagaccc
     2221 tggaggtggc ggagatggtc ctgtcgggtc tcgtgaacaa ggagatcgtc aagaacatca
     2281 acgatatggg cggtaaggcc atcggtcttt ccggcaagga cgggaggctt ctcatggcga
     2341 agcaggtcga ggacagggag gtgggcctcg taggagagat cacctccgtc gatgtcgcca
     2401 tcataaagga tatcagcagg cacggctaca tccccgtcat agctcccatc gccgacgggg
     2461 tggacggcaa ggcatacaat atcaatgccg acacggcggc gggcagcatc gccgcggccc
     2521 tctcggcgga gaaattcata cttctcaccg atgtgcaggg tgtcatgggg gcggacggaa
     2581 agctcatttc cgtcctgaag cgatcggaga tagagaagct tatcgatgac aacgtcgtga
     2641 ccggcgggat gatccccaag gtcacatgct gcaccgaggc gcttaaaggg ggtgtcaggg
     2701 aggcccacgt cgtcgacggc aggacccccc atgccattct cctcgaggta ttcaccgatt
     2761 cggggatagg caccgagata acaggagact ag
//
LOCUS       DAOPOA010000272         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39335_length_2787_cov_3.863470, whole genome shotgun sequence.
ACCESSION   DAOPOA010000272 DAOPOA010000000
VERSION     DAOPOA010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39335_length_2787_cov_3.863470"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            459..1688
                     /locus_tag="PKJ17_06495"
     CDS             459..1688
                     /locus_tag="PKJ17_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75658.1"
                     /translation="MKRFIAVMIGMALVCAGLGSAFAAGYSFNALDYPGASETRAYGI
                     NDWGVVVGSHQGAGEVYGHAFVLRGDTWTSFDDPDAAFGTSPRGINDWGKIAGTFGNS
                     IGDVSGFTLAFGVRRRLDYPTAAHTFLGGVNGLGTVVGYYMIPSTRSFVLKGTTYTLL
                     DYPPTTDDPENPTGWLRAHDINDHGVIVGGCTDANGSHGCYSSGGAWVLLDYPDATST
                     EAFGVNNHGIIVGQYTGEDGSVHGFSYDGTIWNTLDHPGATSTIAYGINDRGTIVGTF
                     EDANGTHGFLAKAVSDLTSLAIDIRPWSAENVLHLLERGLLPVAVLGGNAFDALGTID
                     LGTISFGRTGDEDSMAFCSPWDWDVNADGYQDLICLFWVQKAGFQCGDTGGILKGMTQ
                     DGTTLEGRDAVKVIPCR"
     gene            complement(1793..>2787)
                     /locus_tag="PKJ17_06500"
     CDS             complement(1793..>2787)
                     /locus_tag="PKJ17_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOD75659.1"
                     /translation="GGSIIRALKAQVDARKIPVMMNVKLKALVRQKPLEGDIMGAVVT
                     EKGKDVYFKAQKAVILATGGFAADVAMRSKHDPRLTAEVPTTNVPWATGEAIVIAENE
                     GADVHGMDYIQMLIACNYFTKKYGSLTNLGVDHALFVNLDGKRFVAEDQRRDVMAEAV
                     LKQKNKVLLWVADEKCSKRFNPKMTEEIIKDGLAFRANTLEELAKILNAKLEVPEQAF
                     IESVAKYNESVTKGKDEEFGKKPQNLKPVTTAPFYASPTQAGVHHTMGGIQTKGTTCQ
                     VIDRENKIVPRLYAAGEVTGGVHGANRVGGNATVDCIVFGRNAGINAAKEKSWS"
BASE COUNT          518 a          863 c          807 g          599 t
ORIGIN      
        1 ccggcatcga tgccagccga tcctgtggtc ccgcggcatc tcttcccgta gagaccccat
       61 gccgtattac tccgcctctc catggttctc ccacaatagc caggtgaatt gatcaacggg
      121 acggcgctcc cgcgaggata cgctccggac gattgaccgg acccggagac gagcacccgt
      181 cgagagcaag ggcccgggag tcccggtccc ggtgacagac acggaaaatg cgacagggaa
      241 ggtggtgaaa ttaccacaaa aaaaatgtcc ctttggcgac agacacgaac gggcactcca
      301 tgtagcatgt aatcagtctt ttgcctgcat tttgttattc accctgagat acgacacctg
      361 cagcgaacac ctcaccttgt tctgcttacg cgaccatctc ccgcgaaacg cccccgcgcc
      421 acctgtggaa caaagattca caaaaggagg ggaaacagat gaaacgtttt attgcggtca
      481 tgatcggtat ggctcttgtg tgtgccggac tgggcagtgc tttcgccgcc ggttacagct
      541 tcaacgctct cgactatcct ggggcttcgg agacgcgggc gtatggcatc aacgattggg
      601 gggtcgtcgt cgggtcccac cagggcgccg gggaggtcta cggccatgct tttgtcctga
      661 gaggtgatac gtggacatct ttcgacgatc ccgatgccgc cttcgggacc agtccgcgag
      721 gtatcaatga ttggggaaag atcgctggga ccttcgggaa cagcattggg gatgtcagcg
      781 gttttaccct tgctttcggg gtgcgcagac gccttgatta tcccaccgcg gcgcacacct
      841 tcctcggggg tgtcaatggc ttgggcacgg tggtcgggta ctacatgatc ccttcaacgc
      901 gcagtttcgt gctgaaaggt acgacctaca ccctgcttga ctatcctccc accactgatg
      961 acccggagaa cccgacggga tggctccgtg cccatgacat caacgatcac ggtgtgatcg
     1021 tcggaggttg caccgacgcg aacgggtctc acggctgtta ttccagcggc ggggcctggg
     1081 tcctcctcga ctacccggac gcgacatcaa cagaggcctt cggtgtcaac aaccacggca
     1141 taattgtcgg ccagtacacc ggggaggatg gatcggtcca cggtttttcc tacgatggta
     1201 cgatctggaa cacccttgat catccggggg ccaccagcac tatcgcctat ggcatcaatg
     1261 acagagggac gatcgtcggg accttcgagg acgcgaacgg gacccatggc ttcctggcaa
     1321 aggccgtgtc cgacctcacg tcgctcgcca tcgacatcag gccgtggagc gcggaaaacg
     1381 tgctgcacct gctcgagcgg gggctccttc cggttgccgt actcggcggc aacgcctttg
     1441 acgccctcgg cacgatagac ctcggcacca tcagcttcgg acgtaccggg gacgaggaca
     1501 gcatggcctt ctgcagtccc tgggactggg acgtcaacgc ggacggttac caggacctca
     1561 tctgtctctt ctgggtacag aaggccggat tccaatgcgg cgacaccggg ggcattctca
     1621 aggggatgac acaggatggg accacccttg agggacgcga cgcggtgaag gtcattccct
     1681 gccgctaggg ctgtcgtccc acggttcacc ccctttttgg ggccgttcaa aataaggggc
     1741 agagagatct ccctgcccct tgtcacctat tccatcccga atcgtccgga gttcagctcc
     1801 aggacttttc cttcgccgcg ttgatgccgg cgttccttcc gaagacgatg cagtcgaccg
     1861 tcgcgttccc gccgaccctg ttcgcaccgt ggacgccgcc cgtgacctcg cctgccgcgt
     1921 agagccgcgg gacgatcttg ttctccctgt cgatcacctg gcacgtggtc ccctttgtct
     1981 ggatgccccc catcgtgtgg tgcacaccgg cctgggtagg gctcgcatag aaaggagcgg
     2041 tggtgacggg cttcaggttc tgcggctttt tgccgaactc ctcgtccttg cctttcgtga
     2101 cgctctcgtt gtacttcgcg acggattcga tgaaggcctg ttccgggacc tcaagcttcg
     2161 cgttcaagat cttcgcgagc tcctcgagcg tgttcgcgcg gaaggcgaga ccgtccttga
     2221 tgatctcctc cgtcatcttc gggttgaacc tcttggagca cttttcatcc gcgacccaga
     2281 ggagcacctt gttcttctgc ttgagaacag cctccgccat gacgtcgcgg cgctggtcct
     2341 cagcgacgaa cctctttccg tcgaggttga cgaagagggc gtgatcgaca ccgagattcg
     2401 tcagcgaacc gtatttcttc gtgaagtagt tgcaggctat gagcatctgg atataatcca
     2461 tcccgtggac atcggcccct tcgttctcgg cgatgacgat ggcctctccc gtcgcccatg
     2521 gcacattcgt cgtcggaacc tcggcggtca ggcgcggatc atgcttggag cgcatcgcaa
     2581 cgtcggcggc gaaaccgccc gtcgcaagaa tgacggcctt ctgggccttg aagtagacgt
     2641 ctttcccctt ctccgtgacc accgctccca tgatatcacc ctcaaggggt ttctgccgga
     2701 cgagggcctt gagcttcaca ttcatcatga cggggatctt tctggcatcg acctgggcct
     2761 tcagggcccg tatgatcgaa cctcccc
//
LOCUS       DAOPOA010000273         2778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39495_length_2778_cov_3.002571, whole genome shotgun sequence.
ACCESSION   DAOPOA010000273 DAOPOA010000000
VERSION     DAOPOA010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39495_length_2778_cov_3.002571"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKJ17_06505"
     CDS             complement(<1..744)
                     /locus_tag="PKJ17_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOD75660.1"
                     /translation="MDNAYKSILADALQGFVREREALRYHCKKEAACLKRFDRFCIDI
                     GHNEVCLSRGLAMRWAEKKPYESDDAQGRRIRLIRMLARYMAKSGYQAYIYPDHLGVR
                     RSETYQPYLFSEAELARFLARVDQCLPLRNSPYRHRIFPLLYRMLYGCGLRVSEALHL
                     TVGDVNAEEGILTIRNAKFHKDRMVPMSPPLTERCRIYMEAMHPLGQPEYPFFPSPYG
                     GRYPERTVYAYFRRFIWEAGISHGGRGKGP"
     gene            complement(737..1978)
                     /locus_tag="PKJ17_06510"
     CDS             complement(737..1978)
                     /locus_tag="PKJ17_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOD75661.1"
                     /translation="MKPERSIRNLVAEVLGELERLNYSQNTRTGYRRFYRRVIDFADS
                     AGENVYSESLGNRFLQTKYKFDLDSYTVSLHRSFRNEARCIRVLGDYQLHGSILRRRT
                     TKVPYERTPQFAEALKAYYEECGRRNYSHQGMRGRIYRTELFIDYLDDHGITLLAPVT
                     GRHISDYIRTIAGYHRKSISAILTTLRSFLKFLYIAGYHEKDLSGDVARLRQPHYPKI
                     PSTWRPDDVRRLLASVDRGNPSGKRDYAILLIVSRLGMRVKDIRELRLSNLNWATRTI
                     EIVQHKTKQSVSYPILDDIGWAIIDYLKNGRPKTESPHLFVRHNAPFEAFGACANLHH
                     IIAGYARLAGIDLRTGASRGMHSLRHTLASTLLEQGTPLPVISEILGHVSTLSTNVYL
                     KVDFEHLRACALDPDEVFCHG"
     gene            complement(2117..2440)
                     /locus_tag="PKJ17_06515"
                     /pseudo
     CDS             complement(2117..2440)
                     /locus_tag="PKJ17_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2554..>2778
                     /locus_tag="PKJ17_06520"
     CDS             2554..>2778
                     /locus_tag="PKJ17_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75662.1"
                     /translation="MRSDFLPVIEHQEIAIEADAHRHYRPSHLVGDTVAVAVNIDVAI
                     PLGEPLLTVRGVVPRERERPERFTKEAFLDD"
BASE COUNT          569 a          782 c          760 g          667 t
ORIGIN      
        1 cggtcccttg cccctgccgc cgtgagaaat ccctgcttcc cagataaacc tgcggaagta
       61 cgcatagacc gttctctcgg gatatcgacc gccgtaggga gagggaaaga agggatactc
      121 aggctgtccg agggggtgca tcgcctccat gtagatgcgg cacctttcag taagcggggg
      181 actcatggga accattctgt ctttgtggaa tttggcgttt ctgatggtca gtatcccctc
      241 ttcagcattt acatcgccca cggtcaggtg aagcgcttcg gatacacgaa gcccgcatcc
      301 ataaagcatt ctatagagca ggggaaagat gcgatggcga tatgggctgt ttcttagagg
      361 caggcattga tccacccggg caaggaacct ggcaagttcc gcttcagaaa aaagataggg
      421 ctgataggtt tccgaccggc gtacgccgag atggtccgga tagatgtacg cttggtatcc
      481 cgacttcgcc atgtacctcg cgagcatgcg gatgagcctg atcctgcggc cttgcgcgtc
      541 gtcagactca tatggcttct tttcggccca cctcatggcc aaccccctcg acaggcatac
      601 ctcgttgtgg cctatatcga tgcagaacct atcgaaccgc ttcaggcagg ccgcctcctt
      661 tttacagtgg tatctcaacg cctccctctc ccgcacgaac ccttgtagcg catcggcaag
      721 gatgctcttg tatgcattat ccatggcaga acacctcatc gggatcgagg gcacatgccc
      781 tgaggtgctc gaagtcgacc ttcaggtaca cgttcgtgga cagggtactc acatgtccca
      841 ggatctccga tattaccggc agcggcgtcc cctgctcgag aagagtgctt gccagggtat
      901 gcctcaggga atgcatcccc cgggacgctc ccgtcctcag gtctataccg gcgaggcgtg
      961 cgtagccggc aatgatatgg tggaggttgg cgcatgctcc gaaggcttcg aaaggcgcgt
     1021 tgtgacggac aaagagatgg ggtgattccg tttttggccg gccgttcttc aggtaatcga
     1081 taatggccca gccgatgtca tcaagtatgg gatagctcac actctgcttt gttttgtgct
     1141 ggacgatctc gatggtcctc gtcgcccagt tcaggttgct caacctgagt tctctgatgt
     1201 ccttcaccct catgcccagt cgggagacga tcaaaagaat cgcgtaatcc cttttgccgc
     1261 tggggttacc cctatccacc gacgcaagaa ggcgtcttac atcatcaggt ctccaggtac
     1321 tcgggatctt tgggtaatgg ggctgtctca gcctcgccac gtcgccggac agatcttttt
     1381 cgtggtagcc tgctatatac aagaacttca agaacgacct gagcgtggtc aggatcgccg
     1441 aaatagactt cctgtggtaa ccggcgatag tccttatgta atcggatata tgccggccgg
     1501 tcactggagc cagcaacgtg atgccgtggt cgtccaggta atcgatgaac agttctgtcc
     1561 ggtagatgcg gccccgcatc ccttggtgag agtaatttcg gcgcccgcat tcttcgtagt
     1621 aagccttgag cgcctcagcg aactggggcg tcctctcgta aggcaccttt gtggttctcc
     1681 tccgcagaat cgagccatgg agctggtaat ctcccaggac cctgatgcag cgagcttcgt
     1741 tcctaaaaga gcggtgcaaa gagacggtat agctgtccag atcgaatttg tatttcgttt
     1801 gtaagaacct gttgcccagg ctctctgaat agacgttttc tcccgcgctg tcggcaaagt
     1861 cgattacccg acggtagaat cttcggtacc cggtgcgcgt gttttgtgag taattgagtc
     1921 tttcgagctc tcccagtact tctgctacga ggttcctgat ggatctttcc ggcttcatga
     1981 aaaccctcct tcattatgat ggatgcctac gcatcgctct catcgtgaag gattatggaa
     2041 agtctttcta tcttaaagac agcgatttca atcagtttgg ccagccactt tgcataaccg
     2101 attgctttgc ataattgcca acccccggag gtcctaacac gatgatgttc tcacccctgc
     2161 cgacgaaggc cagtgtcgaa agctctctga tcaccttccg gtccagagat ggctggaagg
     2221 tgaagtcgaa ctgttctaag gtcttcatcc aggggaagcg ggccatggcg aggcggaact
     2281 cgaccccttt ctggtgacgg ccgctccatt ctgtctggag tacttccgtg aggaactccc
     2341 ggtaccccag gtccttcttc gacgcctgct cgcagatcgc atcaagctgc gcgtgcaggt
     2401 gttccagttt catccgttcg agcagtccct ctagttgcat aggacctcct catagacgga
     2461 gagatcccga cgctcaacat ggagcgtctg accccagagt gaggcatggt ggccggggac
     2521 cgtcgaccat ccttcgttcg tttcacggag tctatgcgca gcgattttct ccccgtcata
     2581 gagcaccagg agatcgccat cgaggctgat gcgcaccgtc actatcgccc ctctcacctg
     2641 gtcggggata ctgtagcggt tgccgtcaac atcgatgtag ccatcccact gggcgagccg
     2701 ctgctcacag tacgaggtgt cgtaccgcgt gagagggaga ggcccgagcg tttcacgaag
     2761 gaagcgttcc ttgacgac
//
LOCUS       DAOPOA010000274         2777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39508_length_2777_cov_4.312270, whole genome shotgun sequence.
ACCESSION   DAOPOA010000274 DAOPOA010000000
VERSION     DAOPOA010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39508_length_2777_cov_4.312270"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="lspA"
                     /locus_tag="PKJ17_06525"
     CDS             <1..399
                     /gene="lspA"
                     /locus_tag="PKJ17_06525"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOD75663.1"
                     /translation="DLPVMSEIKVTSFLSIVHVRNLGGVFGILNESGYAKYIFTVVPV
                     LVAAALVYILIRYTMGKAKTFALCLILAGALGNIYDRIFYGSVVDFLDFHYGKYHWPA
                     FNVADIALSIGIGLLILLELTEAKGRQPSG"
     gene            444..674
                     /locus_tag="PKJ17_06530"
     CDS             444..674
                     /locus_tag="PKJ17_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75664.1"
                     /translation="MDDILEEERKLRRLRFVVDFALEFIRTQDIPHDHAMHIVEGVRK
                     QALKLFPGKDETFDIIYAPRFKRALNEKYKRD"
     gene            complement(825..1982)
                     /locus_tag="PKJ17_06535"
     CDS             complement(825..1982)
                     /locus_tag="PKJ17_06535"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOD75665.1"
                     /translation="MAARRNGFRIFVVSAIVALCLALIAAVFLVKYANMIARAGIEKA
                     MGKDFSIGSIELKWGSVRVHGVAMKDRAGKEVIRVGNLVVKASFMSLLRQKYVISSVL
                     MEKPYIYVEIDRKGDLVSPVFPAGDEIARKAGRKAGDSRRPEGKQRPVILKKIVIVNG
                     SVDYLDRKSPKVPVLTRVRSINAEVRNLTVPFTDDLTHYVLRAAIPEGRSTASVKSEG
                     GIRFQSKDMECTAAVRELDITHFKPYYHGSQSINMTRGLLDLDVRAKIVSRRIHAPGK
                     AVLKGLEFGSGHGLGDRFMGVPVTLVVAFLKKSGDQIPVDFVISGDLDNPKFNIAENF
                     ASRLSFSIAEKLGFSVKDIGESVFGLGTEGTKKIGESIEGIGEGIKKIFKR"
     gene            2090..2404
                     /locus_tag="PKJ17_06540"
     CDS             2090..2404
                     /locus_tag="PKJ17_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HOD75666.1"
                     /translation="MSVLFEFAMFPTDKGESVSAFVSRLLRVIDEGTAAYRLTPMGTV
                     VETDTMDQALSIIKKCYEQLEPDCGRVYSTVKFDIRKGASGRLEGKIASVEAKVGKKL
                     KT"
     gene            complement(2556..>2777)
                     /locus_tag="PKJ17_06545"
     CDS             complement(2556..>2777)
                     /locus_tag="PKJ17_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75667.1"
                     /translation="YRYAIGYLKPFSEAAPRHPRIHAILGQCYEAVGDYQSAYGQYTM
                     QLKVAPNNDAGGVARSRSRAIEMQFAPKK"
BASE COUNT          599 a          818 c          739 g          621 t
ORIGIN      
        1 gatctccccg tgatgagcga gataaaggtg acgtccttcc tctccatcgt ccatgtgagg
       61 aacctgggag gtgtcttcgg tatcctcaac gagagcgggt acgccaaata catcttcacc
      121 gtggtacccg tcctcgtcgc cgccgccttg gtatacatcc ttatccggta cacgatggga
      181 aaggcgaaga ccttcgcgct gtgcctcatt cttgccggcg ccctgggcaa catatacgac
      241 cgcatattct atggaagcgt cgtcgacttt ctcgacttcc actacgggaa ataccactgg
      301 cccgccttca acgtggctga tatagccctt tccatcggca tcgggctcct catcctcctc
      361 gaactgaccg aggcaaaggg caggcaaccc tccggctgaa ggcacatcgc aggcggtgcc
      421 ggaagccata aagagggaac gtcatggacg atatcctgga agaagagaga aagctcaggc
      481 ggcttcgttt cgtggtcgac ttcgcgctcg agttcatccg cacccaggac atcccccacg
      541 accacgccat gcacatcgtc gagggggtgc gcaagcaggc gctcaagctc ttccccggaa
      601 aagacgaaac cttcgacatc atctacgccc cccgcttcaa aagggcgctg aacgagaagt
      661 acaagaggga ctgaaaagac ggcttgaatg gcttgagtcg cttgaatcgt tcgaaggttg
      721 aggaaacaac ctctggattc cccggtgcgc gggaatgatg gccagaaaag gttgttcctg
      781 tgactttcaa gctgttcaag cgactcaagc cattcaagcc gtctttatcg tttgaatatc
      841 ttctttatcc cctcgccgat accctcaata ctttcaccta tctttttcgt gccttcggtg
      901 ccgaggccga agaccgactc cccgatgtcc tttacggaaa aacccagttt ttcggcgatg
      961 ctgaaggaga gtctgctagc gaagttctcc gcgatgttga acttcggatt gtcgagatcg
     1021 cctgaaatga cgaaatcaac ggggatctga tccccgctct tcttcaggaa ggccacgaca
     1081 agcgtgacgg gcacacccat gaacctgtca cccagaccgt ggccgcttcc gaattccaga
     1141 cccttgagca ccgcctttcc cggcgcgtgg atcctgcggg aaacgatctt cgcccgcacg
     1201 tccagatcaa gaagacccct ggtcatgttt atgctctgac ttccatggta gtagggcttg
     1261 aaatgcgtga tgtcaagctc ccttaccgcc gccgtgcact ccatgtcctt gctctggaac
     1321 ctgatcccgc cttcgctctt cacgctcgcc gtgctcctgc cctcagggat ggcggccctg
     1381 aggacgtaat gagtgagatc gtccgtgaaa ggcaccgtca ggtttcgcac ctccgcgttt
     1441 atgcttcgca cccttgtcaa gacgggaacc ttcggagact tgcggtcgag atagtccacg
     1501 gacccgttta ctatgacgat cttcttgagt atcacaggac gctgtttccc ttcgggccgg
     1561 cggctatccc cggcctttct cccggctttc cttgctattt cgtcaccggc cgggaagaca
     1621 ggacttacga gatcgccttt tctgtcgatc tcgacgtata tgtaaggttt ttccatgagg
     1681 acgctggaaa taacgtactt ctgcctgaga aggctcatga agctggcctt gaccacaaga
     1741 ttcccgaccc gtatcacttc tttgccggcc ctgtctttca tcgcaacacc atggacacgg
     1801 acgcttcccc atttcagttc gatgctccct atagagaagt ccttccccat ggccttctcg
     1861 ataccggccc tggcgatcat gttggcgtac ttcacgagaa agacggccgc gatcagggca
     1921 aggcagagag ccacgatggc agagacaacg aatatcctga acccattccg cctggcagcc
     1981 attttcccat ccttttcgaa atgttttata ctagtataca gaacgggaac ccggaaggaa
     2041 agacaggaga accgccgcgg cttcccgtaa ccgggaaagg aggaccttca tgtccgttct
     2101 atttgaattc gcaatgtttc ccacagacaa gggcgagagc gtcagcgcct tcgtgtcgag
     2161 gctcctcagg gtcatcgacg agggtacggc cgcctaccgc ctcactccaa tgggcaccgt
     2221 ggtggagaca gacaccatgg accaggcact gtccatcata aaaaagtgct acgagcagtt
     2281 ggaacccgac tgcggccgcg tctattccac cgtcaagttc gatatccgca agggcgcctc
     2341 aggcaggctc gaaggcaaga tagcctccgt cgaagccaag gtggggaaga aactgaaaac
     2401 ctgaaagacg gcttgaatgg cttgaatcgc ttgagtggct tgagggttat agaagcaatg
     2461 cccggattta gccggccgcc ggagcaacag cgggctatgg ctttcttctt ttgactctca
     2521 agcgattcaa gcgattcaag cgattcaagc cgtctttatt ttttgggtgc gaactgcatc
     2581 tcaatggccc gggagcgtga gcgcgcgaca ccgcccgcgt cgttgttcgg ggcgaccttg
     2641 agctgcatgg tgtactggcc gtatgccgac tggtagtcgc ccactgcctc gtagcactgt
     2701 cccaggatgg cgtggatcct gggatgcctg ggagcggcct ccgagaaggg cttcaggtag
     2761 cctatggcgt acctgta
//
LOCUS       DAOPOA010000275         2774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39559_length_2774_cov_3.539904, whole genome shotgun sequence.
ACCESSION   DAOPOA010000275 DAOPOA010000000
VERSION     DAOPOA010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39559_length_2774_cov_3.539904"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKJ17_06550"
     CDS             <1..308
                     /locus_tag="PKJ17_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75668.1"
                     /translation="QTWLTPHSSILQVAVETGVPGVLVFIILNILAIRHLRKVRNDRD
                     HPLSRPAFFVELAFYGFWSGGLLLSHGYSVNLYFLLGISAAIRHLYTNSPGPEPLGS"
     gene            327..1058
                     /locus_tag="PKJ17_06555"
     CDS             327..1058
                     /locus_tag="PKJ17_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD75669.1"
                     /translation="MQRAISSLRWEKAQVYERNFWSRQVSERPGEVSRYRWYEDRAQG
                     IWRRVKPLLASRTRISVLEIGPGPVGIVNYLEADERHALDPLEDYYSSQPEFERVRDR
                     GVMRHNGTGEDVLSLGKTFSFTILDNVLDHMKDPARVLRGIHRSLEPGGVVFISLNIY
                     TRFGALVRNIMECLEIDKGHPFNFSRSSVVTLLRKSGFKVLWSQTEDYRTQKKEYRRS
                     GDLRKVLKSSLGVVDFRFDAFCRKV"
     gene            1058..2362
                     /locus_tag="PKJ17_06560"
     CDS             1058..2362
                     /locus_tag="PKJ17_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD75670.1"
                     /translation="MALRYLQHLCAWPVICRNQWMTPGELVAMQERALRRLVAHAYAR
                     VPHYRRLFDSEGLHPGSIVTLDDLRKVPVLTKEMVRAGYPGDILVSGLDRSECSPRMT
                     SGSSGNKLEVMLDHRVAALYRLMQLRQLTGLGYRPWDTMAYVRFTPPVTSIVPQKMGL
                     FRRSFIPLEWGPERQAEEVLRTRPRIINAYPSVLYLLAKTLGREDGRRLKLKFLMSNS
                     ELLTAHARECMEDVFGCKVYDDYSCLEFSAIAFECRMQKLHVAADNVIVEVLDDRGDR
                     LPAGEPGRMIVTSLNNYSMPYIRYEIGDVGVLSGGKCPCGRRFPVLEAIVGRCDDFLI
                     TPSGRLVDPQTVVFQIETIPEVREFRVIQQEDGSLTVSIVPEGVVEFMRIREQIAEKL
                     KVLFGGTLPVDVIETTALDRGSTGKLRSVFSRRSRDVRASPG"
     gene            2528..>2774
                     /locus_tag="PKJ17_06565"
     CDS             2528..>2774
                     /locus_tag="PKJ17_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75671.1"
                     /translation="MARSLKKCFIYLALLGLFMPGISGGSFADVIIDNGGAGTSYTGT
                     WAASGGTNPYGASSLWSRNGTTYTFTMSGQTAGTYEVL"
BASE COUNT          540 a          822 c          863 g          549 t
ORIGIN      
        1 gccagacgtg gctgaccccg cattcctcga tcctccaggt agccgtggag acaggggtac
       61 ccggcgtcct tgtcttcatc attctcaaca tcctggccat acgtcacctg cgcaaggtgc
      121 gcaacgacag ggaccatccc ctgtcgcgac cggccttttt tgtggagctg gccttctacg
      181 gattctggtc gggaggcctt ctgctcagcc acggctactc cgtcaatctc tacttcctgc
      241 tggggatctc cgctgccata cggcatctgt acacgaactc tcccggcccc gagcctctcg
      301 ggtcctgaag aggaacagga gggaacatgc aacgggccat ttcaagcctg cgatgggaaa
      361 aggcgcaggt gtacgagagg aatttctggt cgagacaggt ctcggagagg ccgggcgagg
      421 tgtcccggta caggtggtat gaggaccgcg cgcagggtat ctggaggcgc gtgaagcccc
      481 ttctggcctc acggacacgt atcagcgttc tcgagatagg gccggggccg gtgggtatcg
      541 tcaattatct cgaggccgat gaacgccacg ccctcgaccc cctggaggat tattacagct
      601 cacagcccga gttcgagcgc gtcagggacc ggggcgtcat gcgccacaac ggtaccggcg
      661 aggatgtcct ttccctcgga aagaccttct cgttcaccat tctcgacaac gtcctcgacc
      721 acatgaagga cccggcgcgc gttctccgcg gcattcacag gagcctcgaa cccgggggag
      781 tcgtgttcat cagcctcaat atctatacca ggttcggtgc cctcgtccga aatatcatgg
      841 aatgcctcga gatcgacaag ggccacccct tcaacttctc ccggtcctcc gtcgtcactc
      901 ttcttcgcaa gagcgggttc aaggtcctgt ggtcgcagac ggaagactac cgcacccaga
      961 aaaaagagta caggcgctcg ggagatctaa gaaaggtgct caagagctct ctcggcgtcg
     1021 ttgatttcag gttcgacgcc ttttgcagga aggtctgatg gcgcttcgct atctccagca
     1081 tctgtgtgcc tggccggtca tctgccggaa ccagtggatg acgcccggcg agctcgtcgc
     1141 catgcaggag cgggcgttga ggcgccttgt tgcccacgcg tacgccaggg tcccccacta
     1201 tcgcaggctc ttcgactctg agggactgca ccccggcagc atcgtcaccc tggacgacct
     1261 gaggaaggtc ccggtcctga caaaggagat ggtgcgtgcc ggctatcccg gggacatcct
     1321 tgtgtccggt ctcgaccggt ccgagtgctc gccgcgcatg acgtccggat cgtcgggcaa
     1381 caagctggag gtgatgctcg accacagggt ggccgcgctg taccgtctca tgcagttgag
     1441 gcagctcacc ggcctgggct acaggccctg ggacacgatg gcgtacgtgc gttttacacc
     1501 tccggtgacc agcattgtgc cgcagaagat ggggctcttc aggcgttcct tcattcccct
     1561 cgagtggggt ccggagcggc aggcggagga ggtcctccgc acgaggccgc ggatcatcaa
     1621 cgcctacccg tcggtgctct acctgctggc gaagacgctg ggcagggagg atgggaggcg
     1681 cctcaagctc aagttcctca tgtcgaactc ggaactcctg acggcgcacg cgagagaatg
     1741 catggaagac gtgttcgggt gcaaggtcta tgacgattac tcgtgcctgg aattctccgc
     1801 gatagccttc gaatgcagga tgcagaaact tcatgtcgcg gcggacaacg tgatcgtcga
     1861 ggtgctcgac gacaggggcg accggcttcc cgcgggggag cccgggagga tgatcgtcac
     1921 atccctcaac aattattcca tgccctacat ccgttacgag ataggcgatg tcggggtcct
     1981 gtccgggggg aaatgcccct gcggcaggcg cttccccgtt ctcgaggcca ttgtcggaag
     2041 gtgcgatgat ttcctgatca cgccctcggg acggctcgtc gatccgcaga cggtggtgtt
     2101 ccagatcgag accatccccg aggtgaggga attcagggtc atccagcagg aagacgggag
     2161 cctcacggtg agcatcgtcc ccgagggggt ggtggaattc atgaggatca gggagcagat
     2221 agccgagaag ctgaaggtgc ttttcggcgg cacccttccc gttgatgtta tcgaaacaac
     2281 agcactggac agagggtcaa cgggcaagtt gaggtccgta ttctcgcgga ggtcccggga
     2341 cgtccgggcc tctcccgggt gacgatcgcg acggcggtac cctcaagaaa agagttgaat
     2401 acgtcccttc cgacggaacc gtcatcgccg gtttccacgg aagggtggac agattttttg
     2461 ccgatgcacc gtggggatat gcaagatcgt aggggtcggc gcaaacaagt gataaaagga
     2521 gtgcatcatg gccagatcat taaagaagtg ttttatttat ctcgctttac tgggtctctt
     2581 catgcccggc atcagcggcg gttcctttgc cgatgtcatc attgacaacg ggggtgcggg
     2641 gacgtcctac acggggacct gggccgcctc gggaggcaca aacccctacg gcgcctcgag
     2701 cctgtggtcc cgcaacggta ccacgtacac cttcaccatg agcggacaga cagccgggac
     2761 ctacgaggtc ctca
//
LOCUS       DAOPOA010000276         2770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39639_length_2770_cov_2.529650, whole genome shotgun sequence.
ACCESSION   DAOPOA010000276 DAOPOA010000000
VERSION     DAOPOA010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39639_length_2770_cov_2.529650"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKJ17_06570"
     CDS             <1..509
                     /locus_tag="PKJ17_06570"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HOD75672.1"
                     /translation="WQLPFIGGYKFESPPGVLNLDGCIMFYFAATWVTPAMEEKMVVE
                     GSQYAWAMVDSKGVSLNGSKTDKLHLPPNIPIKNFWSVIVYDNQARSMIQTDQQFPSV
                     SSQDKDFVMNKNGSVDVYFAPKKPAGKVNWIQTVPGKGWNVILRLYSPLEPWFNKTWR
                     PGEIEEVK"
     gene            612..>2770
                     /locus_tag="PKJ17_06575"
     CDS             612..>2770
                     /locus_tag="PKJ17_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75673.1"
                     /translation="MNKEEIRLREAREGKLAWKKWGPYLSERQWGTVREDYSENGDAW
                     NFFTHDHARSRAYRWGEDGLAGLSDEKQHLCFALALWNGKDPILKERLFGLTNSEGNH
                     GEDVKEYYFYLDSTPTHSYMKYLYKYPHAAYPYADLVDTNGRRSREELEYELLDTGVF
                     DDDCYFDVFVEYAKSGPEDILVKITATNRGPQTADMHLLPTLWFRNDWATWITNRTEK
                     PVLRQITGPQGTSAVAAKHSVLGTYYLCCEGDVPLLFTENSTNHARLGLNYPDVGPYL
                     KDGINDFVVNGRQDAVNPAKTGTKVSAHYAGSVGPGASMVVRLRLTDRASAIKKGKRK
                     NDFIPFGPGFDEMLAARLREADEFYASVTPPSVSPDAVNVMRQAIAGMLWSKQYYFFD
                     ADVWLEEHRAHPLHSGSRDFRNRDWFHMVNDDIISMPDKWEYPWYAAWDLAFHTLPIA
                     IVDPDFAKEQMRLMLRGGYLHPSGQLPAYEWNFSDVNPPVHAFATLFLHRTELALHGK
                     ADFDFLKMAFNKLLLNFTWWVNRKDRFGKNVFEGGFLGLDNIGVFDRSAPLPTGGYLE
                     QADGTAWMALYTQNMLELAIELAAEDPIYEDLIAKFGEHFLYIAAAMNKPGHDGMWDE
                     EDGFYYDLLRFPDGSARRLKVRSVVGLLPLCAVTVIEPRHRELYPRVVAAIRERARQM
                     PELAESIHPTGPGHFGIGDRGIFALVNPDRLRRILSK"
BASE COUNT          615 a          785 c          814 g          556 t
ORIGIN      
        1 cttggcaatt gccttttata gggggctaca agtttgagtc accacccggc gttttaaatc
       61 tcgacggatg catcatgttc tattttgccg ccacgtgggt caccccggca atggaagaaa
      121 agatggttgt cgagggctcg caatacgcct gggcaatggt ggattcaaaa ggcgtctccc
      181 ttaacggcag caagacggac aaactgcatc tgccgcccaa tatccctatc aagaatttct
      241 ggtcggtgat cgtatacgac aaccaggcac gttcgatgat ccagacggac cagcagttcc
      301 cgagcgtcag cagccaggac aaggacttcg tcatgaataa gaacgggtcc gtggatgtct
      361 actttgcacc gaaaaaaccg gcagggaagg tcaactggat ccagaccgtc cccggcaagg
      421 gttggaacgt tatcctgcgt ctttacagcc cgctggaacc ctggttcaac aagacctggc
      481 ggccggggga aattgaagaa gtgaaatagg cggcatcacg gcttttatgg gctggtcgaa
      541 ggtcgatgcg aaacaattga tgtataatct aaatagaagg cgagtgtttt acgtccaggg
      601 ggtaaccgga gatgaacaaa gaggagataa ggcttcggga ggcgcgggaa gggaagcttg
      661 cctggaagaa atggggcccc tacctgagcg agcgccagtg ggggaccgtt cgtgaggact
      721 acagcgagaa cggagacgcg tggaacttct tcacccatga ccacgcccgt tcccgagcct
      781 accggtgggg cgaagacggc ctcgctggtt tgtccgacga gaagcagcat ctctgttttg
      841 cccttgcatt gtggaacggc aaagatccta ttctcaagga acgccttttc ggcctaacca
      901 acagcgaggg gaaccacggc gaggacgtga aggagtatta cttctatctc gacagcacgc
      961 cgacccactc ctatatgaag tacctttaca agtatcccca cgcggcctat ccctacgccg
     1021 accttgtgga tacgaacggg cgccgcagcc gggaggagtt ggagtacgag ctcctggata
     1081 cgggcgtctt cgacgatgac tgctactttg acgtcttcgt ggaatacgcg aagtcaggcc
     1141 ccgaggacat cctggttaag atcaccgcaa cgaaccgcgg acctcagacc gccgatatgc
     1201 accttctgcc gacgctctgg ttccgcaacg actgggcaac gtggataacg aaccgcacgg
     1261 aaaaacccgt gctcaggcag atcactgggc cacaagggac gagcgctgtg gcggcaaagc
     1321 attccgtgct ggggacgtac tacctgtgct gcgaaggcga tgttccgctc ctctttacgg
     1381 agaactcaac aaaccatgcc cggctcggcc ttaactaccc cgacgtgggc ccttacctca
     1441 aggacggcat caatgacttc gtggtgaatg gcaggcagga tgcggtgaat cccgcgaaga
     1501 cgggcacgaa ggtgtcagcg cactacgcag gcagtgtcgg ccccggtgct tcgatggtgg
     1561 tacggctgcg tcttaccgac cgggcatcgg ccatcaaaaa agggaaaaga aagaacgact
     1621 ttatcccctt cggaccgggt tttgatgaga tgctggccgc ccggctccgc gaggcggacg
     1681 aattctacgc gtcggtgacg cccccttccg tttcacccga tgcggtcaat gtgatgcgcc
     1741 aggctatcgc aggcatgctc tggtcgaaac agtactattt tttcgatgcc gatgtctggc
     1801 tggaggagca cagggcccac ccccttcata gcgggagccg cgacttccga aaccgggact
     1861 ggttccacat ggtgaacgac gacatcatct ccatgcccga caaatgggaa tatccctggt
     1921 acgccgcctg ggacctcgcc ttccataccc tgcccattgc gatcgttgat cccgacttcg
     1981 ccaaggaaca gatgaggctg atgctgcgcg gaggctatct ccatcccagc gggcaactcc
     2041 ccgcctacga gtggaatttc agcgatgtca acccgccggt gcatgccttt gcgaccctct
     2101 ttcttcatcg caccgaactg gccctgcatg gcaaggccga tttcgatttc ctcaagatgg
     2161 ctttcaacaa gctcctcctg aactttacgt ggtgggtgaa ccgcaaggac cgcttcggca
     2221 agaacgtgtt cgagggaggc tttctcggcc tcgataacat cggcgttttc gaccggagcg
     2281 cccccttgcc cacgggaggc tacctggagc aggcggatgg cacggcgtgg atggcccttt
     2341 acacacagaa catgctggaa ctcgccatag agcttgccgc cgaggaccct atctacgagg
     2401 atctgatagc caagttcggg gaacacttcc tctatatcgc tgccgccatg aacaagcccg
     2461 ggcatgacgg catgtgggat gaggaggacg gcttctatta tgacctgctg cggtttcccg
     2521 acgggagtgc gaggcggctc aaagtgcgct cagttgtggg acttttgccg ctttgcgccg
     2581 tgacagttat cgagccaagg catcgggagc tttacccgcg ggtagtggcg gctatcaggg
     2641 aacgggcgcg ccagatgccg gaacttgcag agtccattca cccgacaggt ccgggccact
     2701 tcgggatcgg ggatcggggc atttttgccc tcgtcaatcc ggatcgccta cggcggatac
     2761 tgtccaagat
//
LOCUS       DAOPOA010000277         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39689_length_2767_cov_3.273230, whole genome shotgun sequence.
ACCESSION   DAOPOA010000277 DAOPOA010000000
VERSION     DAOPOA010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39689_length_2767_cov_3.273230"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PKJ17_06580"
     CDS             complement(<1..850)
                     /locus_tag="PKJ17_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75674.1"
                     /translation="MYVEVGPASLVIRGEKNGKAHAFLRKEVESLVADILRDIRECLP
                     VLRQRAHRIRNVSALPEVAKRMTAAAKMVSETTLTPMAAVAGTVADMVKEHFTARDID
                     LLMVNNGGDLSIFNATGRALTVGIGDIVTGKVTPYVIRLKDMRDLGMATSGFGGRSLT
                     LGIADTVTAIAGTGAVADAAATHICNRTTIEAGAVVRKAAELVDPETDIPDEDVTVEV
                     GPLSDALIAQALARGLGTARDLTDRNIIDYAVVALKGSIVTTETGNKNKNITLEVQDG
                     NQEDRHH"
     gene            complement(859..2382)
                     /locus_tag="PKJ17_06585"
     CDS             complement(859..2382)
                     /locus_tag="PKJ17_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD75675.1"
                     /translation="MKIDLDKCKGCGLCEEVCPLGVITLKDRKAAIGPGCVECKTCMK
                     VCPEAAFTPETPADHPMCTSCPIMCRIPEGGHGACMRYYNEKGEILRVGRVHSYEEVM
                     DLVREGTGAGPIGTPLITGIGSGTTYPDFRPSPFIVSGVKDGIDIVTVVTEAPLSYSG
                     VKLKVDTDLHVGEEGKKVYVRRKGKRHIGHLCTEEYGSKILSLGGVNILTSKDGLFAA
                     RVLHEFLQGKKIKVEVEDGTKLELALGMEPEIGGIVEERMRVGCGSATTGLFAPYMFD
                     AADEVIVLDGHITGLFSEHPSGRYLGKPRSPIQLTGQKSTEGRYIPNKGSGWGGTDIV
                     NPLDVIEDVDRSKCFDGMTLLITETTGRKSAFYRWQKDRFVEGAPTPEAVTFLNVLKD
                     SCELSRVSAVFAAGVGGSARAGVTKNPIRLTRAVHDGRVSVTVGGARPFIFPGGGINF
                     LVDVEKIKHGSIYVSPTPSFILPIEYTMTLETFREIGGHIEAIRPVGEALQRKDKDR"
     gene            complement(2404..>2767)
                     /locus_tag="PKJ17_06590"
     CDS             complement(2404..>2767)
                     /locus_tag="PKJ17_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75676.1"
                     /translation="KGMLKRLILGNDAPSGTGVIPLGIWRMVNFMSSLCDIPAETAVC
                     FATGNTKNVFGLAQGIVAPGCDADLQIIDAPMGSAGKDAKAAIEVGDIPGIGMVLIDG
                     AVKTQVSRNTPPPVRKTA"
BASE COUNT          434 a          891 c          824 g          618 t
ORIGIN      
        1 gatggtgacg atcttcctga tttccatcct gcacctccag cgttatattt ttgttcttat
       61 tgcccgtctc tgttgtgacg atgcttccct tgagcgccac gacggcgtaa tcgatgatgt
      121 tcctgtccgt gaggtcccgc gcggtcccga gtcctcttgc aagcgcttga gcgatgagcg
      181 catcggacag agggcccacc tccacggtca catcctcatc gggtatgtcc gtttcggggt
      241 ccacgagctc ggccgccttg cggaccaccg cgccggcctc gatggtggtg cggttgcaga
      301 tgtgcgttgc cgccgcgtct gcgacggcgc ccgtcccggc gatggccgtc acggtatcgg
      361 ctatgcccag ggtcagactc ctgccgccga acccgcttgt cgccatgccg aggtctcgca
      421 tatccttgag ccgtataaca taaggcgtta cctttcccgt cacgatgtca ccgatcccga
      481 ccgtgagggc cctgcccgtc gcgttgaaga tggacagatc accgccgttg ttcaccatga
      541 gaaggtcgat gtctcgtgcc gtaaaatgct ccttcaccat gtccgccacg gtgccggcga
      601 ccgccgccat gggtgtcaac gtcgtctcac tgaccatctt tgccgctgcc gtcattcgtt
      661 ttgccacctc ggggagggcg gatacgttcc tgatcctgtg cgccctctgc ctcaggacgg
      721 gaagacattc gcggatgtcc ctcaggatgt cagcgacgag actctccacc tctttcctga
      781 gaaaagcatg ggccttgccg ttcttttcgc cccgtatcac aaggctggcg gggccgacct
      841 cgacgtacat cggtccacct aacggtcctt gtctttcctc tgaagcgctt cccccaccgg
      901 cctgatggct tcgatatggc ccccgatctc cctgaacgtc tcgagcgtca tggtgtactc
      961 gatggggagt atgaaggaag gggtcggtga gacatagatg ctcccgtgct ttatcttttc
     1021 cacgtcgacg aggaaattga tgccgcctcc ggggaatatg aaaggccgcg cgccgccgac
     1081 ggtgacggac accctgccgt cgtggacggc cctcgtcagc ctgatggggt tctttgtcac
     1141 gcccgcccgg gccgagccgc cgacacccgc cgcgaacaca gcgctcacgc gcgagagctc
     1201 gcaggaatcc ttgaggacgt taaggaacgt gacggcttcc ggggtcggcg cgccttccac
     1261 gaacctgtct ttttgccacc ggtagaatgc cgatttcctg cccgtcgttt ccgtgatgag
     1321 cagcgtcatg ccgtcgaagc atttgctcct gtcaacgtcc tctatgacgt ccagggggtt
     1381 cacgatgtcc gttcctcccc agccgctgcc cttgttggga atataccggc cctcggtgct
     1441 tttctgcccc gtgagctgta tgggggaacg gggcttgccg aggtatctgc ccgacgggtg
     1501 ctcgctgaag agcccggtga tgtggccgtc gaggacgatc acctcgtcgg cggcatcgaa
     1561 catgtagggc gcgaagagcc ccgtcgtggc gcttccgcag ccgacgcgca tccgctcttc
     1621 cacgatcccg ccgatctcgg gctccatgcc gagggcgagt tccagcttcg tgccgtcttc
     1681 gacctcgacc tttatcttct tgccctgaag gaactcgtgg aggaccctcg cggcaaacag
     1741 accgtccttc gatgtgagta tgttcacgcc gccgagggag agtatcttcg acccgtattc
     1801 ctcggtgcag aggtggccga tgtggcgttt gccctttctg cggacgtaca cttttttgcc
     1861 ttcctccccc acgtggaggt ccgtgtcgac cttaagcttc acgccgctgt agctcaaggg
     1921 ggcctcggtg acgacggtga cgatgtcgat gccgtccttg accccgctga cgatgaaagg
     1981 cgagggtctg aagtcagggt aggtcgtacc cgagccgatc ccggtgataa ggggcgtgcc
     2041 gatgggtccg gcgcccgtcc cttcgcgcac gaggtccatc acctcttcat aggagtggac
     2101 ccggcccacg cgcagtatct cacctttttc gttgtagtat ctcatgcagg caccgtgccc
     2161 gccctcgggg atcctgcaca tgatggggca cgaggtgcac atgggatggt ccgcgggggt
     2221 ttccggcgta aaggcggcct cggggcacac cttcatgcag gtcttgcact cgacacaacc
     2281 ggggccgatg gcggccttcc tgtccttgag ggttatcacc cccagggggc acacctcttc
     2341 gcagagcccg cagcctttgc atttatcaag gtctatcttc atatgacacc ccgggctttt
     2401 cccttacgcc gttttcctga cgggcggcgg ggtgtttctg ctgacctgtg ttttcaccgc
     2461 tccgtcgatg aggaccatcc cgatgccggg gatgtccccg acctcgatgg ccgccttcgc
     2521 gtccttgccg gcggaaccca tgggagcgtc gatgatctgg agatcggcgt cgcagccggg
     2581 cgccacgatg ccctgggcga gtccgaagac gttcttcgtg ttccccgttg cgaagcagac
     2641 tgccgtctca gcgggtatgt cgcagaggga ggacatgaag ttgaccatcc tccatatccc
     2701 gagagggatg acccccgtcc ccgagggggc gtcgttgccg aggatgagcc ttttcagcat
     2761 ccccttt
//
LOCUS       DAOPOA010000278         2758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39873_length_2758_cov_3.073622, whole genome shotgun sequence.
ACCESSION   DAOPOA010000278 DAOPOA010000000
VERSION     DAOPOA010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39873_length_2758_cov_3.073622"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..1179)
                     /locus_tag="PKJ17_06595"
     CDS             complement(325..1179)
                     /locus_tag="PKJ17_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75677.1"
                     /translation="MNGCKKVFLLLVVFISFPMFPVLGEEGGKKEEPKVTGSGSLGVY
                     NQYIFRGYEIGKSGLVIQPSLTASYKGLSATFWGNVDTNQRSTKTADFSAESKAFKKG
                     WNETDLTLSYTHTFDKLSLTGGYIYYGTNYCDQTEELFGTIAYDIISKPTLSIYRDIT
                     SYKGTYINLSFGHSIPVHKEITLDLGASFGYFVGESDYWKTYDRSTGTYTGSKYKGFH
                     DGMVKAGLTIPITKAFSIQPVVQYWFPLSGDAGREYPTAGDIKDSYNPNGPVNNNFVY
                     GIGLTYAF"
     gene            1759..2310
                     /locus_tag="PKJ17_06600"
     CDS             1759..2310
                     /locus_tag="PKJ17_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75678.1"
                     /translation="MKRPIPKYLWVITAIAGVLVIGTVDLVTGFEMQFFAFYFWPVSM
                     AAWHIGRGASVWVSVLSALVWFATDMLSGHLHSWHFYVVWNTMVRLFSFVVFGLLVSR
                     VRQLLTEQRTLTEDLRRALSEVKVLETFLPICSRCKKIRDQEGSWQELEEYITAHSNT
                     LFSHGYCPECARKAMIEAGLVEK"
     gene            complement(2413..2628)
                     /locus_tag="PKJ17_06605"
     CDS             complement(2413..2628)
                     /locus_tag="PKJ17_06605"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOD75679.1"
                     /translation="MGTEGRVARVTEIVADSRKSFEDAILNGFRRASKTLRGITGIRV
                     KEQYAEVDNGAILKFRVTLDVIFVLDS"
BASE COUNT          577 a          804 c          707 g          670 t
ORIGIN      
        1 gatcggtttc aagaccgtct tgagatcaag gctcccgctc agcctggtgc tgatatcgaa
       61 gagaagcgaa agctccttta cctcgcgatt ttcctggtcg cccatgtgtg gccccctttc
      121 cggcccggcc ggctcgtgtt cccaatagaa aaaggcgtct tcgcgcgccc tccctggggc
      181 ggacaaagac gccttcgtcc caaaatagaa atggcgttcc ctgtgaacgc cattgtccgt
      241 cacatcactg tgacagggtt attctatata acatcgggat cattgtcaac gagtcttgca
      301 cgactatagg acggtcaatt cgatctagaa agcatatgtc agcccgatac cgtagacgaa
      361 gttgttgttc acgggcccgt tggggttgta cgagtccttt atatcccccg ccgtgggata
      421 ctccctgccg gcatctcccg aaagcgggaa ccagtactgg accacgggct gtatcgagaa
      481 agcctttgta atggggatcg taaggcccgc tttgaccatg ccgtcatgga aacctttgta
      541 cttgcttccc gtgtaagtac ccgtcgatct gtcataggtc ttccagtagt cgctctcccc
      601 gacgaagtac ccgaaagagg cacccaggtc gagggtaatc tccttgtgca ccgggatgga
      661 gtggccgaag gaaaggttga tataggtacc cttgtagctc gtaatgtccc ggtagatgga
      721 aagagtcggt ttcgatatga tgtcatacgc tatcgtcccg aagagttcct ccgtctgatc
      781 gcaatagttg gtcccgtagt aaatgtatcc gcccgtgagg gaaagcttgt caaaggtatg
      841 ggtatagctc aaggtgaggt ccgtttcatt ccatcccttc ttgaaagctt tgctctccgc
      901 ggaaaagtca gccgtcttcg tgcttctctg gttcgtatcc acgttgcccc agaaggtcgc
      961 ggaaaggccc ttgtaggacg ctgtgagaga cggctggatg acgaggccgc tcttccctat
     1021 ctcgtagccc cggaatatgt actggttata gacccccagg gaaccggaac ccgtgacctt
     1081 cggctcctcc ttcttccccc cttcttcacc gagaacaggg aacatcggga aagatataaa
     1141 gacaaccaac aacaggaaaa ccttcttgca accgttcatg atatacctcc gtagatttcg
     1201 ttcccatgcc ttcgcatgcg atcgttgttc atccttgccg tctgcggcct cacgcaccca
     1261 tctccttctc tcaagaacga cagaggcgcc ttcaaaccca ccgtggtcga agacgcctct
     1321 gtcttgcacc gtaacctctg tctgatcaaa agcaatgggc gtgccagaaa ccgcaggcca
     1381 cctgcttttt gtcgtagcta attgactttg gataattgtt tcgagatgtt gcgattagat
     1441 acctaccggg tcaactcctt gacctccccg atcgggcaca tcgtgaacac agttgtatcc
     1501 tacataaaag taactcgccg cggcggcatc ctacacaatt gtaaccgcct gcgggtggga
     1561 gacccttttc cccgggggcg ccccgcgctt cattcctcgg aaacctttgc ttctaagctt
     1621 ttcgcagcgc aggaccaggc agggatcgtg ccggcgacac ctctccttta ttactggacg
     1681 cccgcaggat cctgagatat acttgcctgt tccaccaatg ccggttgttg acccggtcag
     1741 agattcgagg gaagcatcat gaaacgtccc atcccaaaat acttgtgggt aatcaccgcg
     1801 atagccggcg tgctcgttat cgggaccgtc gatctcgtca cggggttcga gatgcagttc
     1861 ttcgccttct acttctggcc cgtatccatg gcagcctggc acataggcag gggcgcctcc
     1921 gtatgggtga gtgttctctc cgcgctcgtg tggttcgcaa cggacatgct ctccggccac
     1981 ctgcattcat ggcatttcta cgtggtctgg aacaccatgg tccgcctctt ttccttcgtc
     2041 gttttcggac tgctggtgtc cagggtacgg caactgctca cggagcagcg cacgttgacc
     2101 gaggacctgc ggcgggccct ctctgaggtc aaggtactcg agacctttct ccccatatgt
     2161 tcccggtgca agaagatccg tgaccaggaa ggttcgtggc aggaactgga ggaatacatc
     2221 accgcccatt cgaacaccct cttctcccat ggttattgtc ccgaatgtgc cagaaaggcc
     2281 atgatcgagg cagggttggt cgaaaagtaa atagagcccc acaatgaact tgtggcgcgt
     2341 cgcttcctgt cgatgtctcg ctgccgtcag cgaccacccg caggaaggct ctcccgcgag
     2401 gccggtattc ggttagctgt cgagcacgaa gatcacatca agggtaacgc ggaatttcag
     2461 gatcgcaccg ttgtccacct cggcgtactg ttccttcaca cgtattcccg tgatgccccg
     2521 cagggtcttc gaggccctgc gaaaaccgtt cagtatggca tcctcaaaac tcttcctgga
     2581 atcggcaacg atctctgtta cacgcgcgac tctgccttca gtccccatgt cctctccttg
     2641 gtggttttcc tggaactgca cgggatacgg tctctccgtg cattgattgc agtatatcat
     2701 cagcttcgca caaacggaag gatatcatta cctgagacag tcatctgaga cccgatcg
//
LOCUS       DAOPOA010000279         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_39977_length_2753_cov_3.904003, whole genome shotgun sequence.
ACCESSION   DAOPOA010000279 DAOPOA010000000
VERSION     DAOPOA010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39977_length_2753_cov_3.904003"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1779
                     /gene="secD"
                     /locus_tag="PKJ17_06610"
     CDS             181..1779
                     /gene="secD"
                     /locus_tag="PKJ17_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOD75680.1"
                     /translation="MIKSLKLRFILIVVFLVVSIVYCLPNVIDLEGVWKKYLPSDKIH
                     LGLDLKGGMHLLLELDTVKLMDNIIDRRMNALKDGMISDGVRFLGIDRKGSTITVSIR
                     PDQKEKLFNVVGNKFPDLKVGTAKPEGDLLNVQFLIPEKELASIKENAVNQALETIRN
                     RIDQFGVSEPVIVKQGENQILVQLPGVKDPDRALELIGKTAQLEFKLVDEEAMARGGE
                     TGAIPQGSEVLKIRSKNRETGVETTAPIVLKKQAVLTGELLTDARVHMGGRDIGSEIS
                     VGLEFNSEGGRIFDRVTGENVGKRLAIVLDNTVYSAPQIRERISGGKASITGGFTLNE
                     AKDLAIVLRAGALPAPVKVVQNITIGPTLGQDSINKGMLATLLATLFVVVFMIVYYRY
                     SGIIANIALGLNLIYLLGVFSAFKATMTLPGIAGIALTIGMGVDSNVLIFERIREELR
                     LGKTVRAAVDGGYAKAWVAIFDSHITTLITAAVLFIFGTGPIKGFAITLIFGMIINLF
                     TAVFGSKTIFDWILVKFKPRTLSI"
     gene            1790..2692
                     /gene="secF"
                     /locus_tag="PKJ17_06615"
     CDS             1790..2692
                     /gene="secF"
                     /locus_tag="PKJ17_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOD75681.1"
                     /translation="MMELIKDTKIDFISFRKKAFVISAILVAVGVFSFIMISFGKANL
                     SIDFTGGTNVQVRFAERVNIGDLRSALEKGGIGDVQIQEVSGTRDFFIKTKLSDTEKE
                     SLTAKITSILSTQLKGAKFEMLGSNMVGPGVGQSLKKYAIIAIVLALLGIIIYIAWRF
                     TFLSGIAATIATFHDVLAIAGVFYLLNKEFNLLIITALLTIAGYSLQDTVVVFDRIRE
                     NSGKMKSRDDFGSIINASINEVLSRTIVTGISTLISLAAIMIFGGEVLFDFAFALFLG
                     LIVGTYSSIFVASPILYAWRQKIR"
BASE COUNT          624 a          736 c          798 g          595 t
ORIGIN      
        1 ggttcaaggg ttcaaggggg tttggggggg gatgggaagg cgggggcaga ttggtcccgg
       61 aagtacctcg cagggcgttg cctgttcgtg aaatttgtct tgacaaacgg ggtgtatggc
      121 tgtaatttgg ttgtactttt tgagctggaa ttgccgaatc caaccgaatg aggttcatac
      181 gtgatcaagt cactgaaact gagatttatt ctcattgtcg ttttcctggt cgtttccata
      241 gtgtactgtc ttcccaacgt gatagacctc gagggtgtct ggaagaaata ccttcccagc
      301 gataagatcc acctcggact cgatctcaag ggcggtatgc acctgctcct ggagctcgat
      361 accgtgaagc tcatggacaa tataatcgac aggcggatga acgccctcaa ggacggcatg
      421 atctccgatg gcgtcaggtt cctcggaata gacagaaaag ggagcaccat caccgtctcc
      481 atacggcccg accagaagga gaagctcttc aacgtcgtcg ggaacaagtt cccggatctc
      541 aaggtgggca ccgcgaaacc ggagggggac cttctgaacg tccagttcct tattcccgag
      601 aaggagcttg cctcgataaa ggagaacgcc gtcaaccagg ccctggagac gatacgcaac
      661 aggatcgacc agttcggtgt ctctgagccg gtcatcgtga agcaggggga gaaccagata
      721 ctggtgcagc taccgggcgt gaaggaccca gacagggcgc tcgagctgat aggcaaaacg
      781 gcacagctgg aattcaagct cgtcgacgaa gaggccatgg cccggggcgg cgagaccggg
      841 gccatacccc agggcagcga ggtcctgaag atcaggtcga agaacaggga gacgggagtc
      901 gagacgacgg cccccatcgt cctcaagaaa caggccgttc tgacgggcga actgctgacg
      961 gacgcccggg tccacatggg cggcagggac atcggcagtg agatatcggt gggtctcgaa
     1021 ttcaacagcg aaggcgggag gatcttcgac agggtgacgg gggagaacgt cggcaagagg
     1081 cttgccatcg tcctcgacaa caccgtttac tcggcgccgc agatccgcga gaggatatca
     1141 ggcggcaagg cctccatcac cggaggtttc acgctcaatg aggcaaagga cctcgccatc
     1201 gtgcttcgcg cgggcgccct cccggcccct gtgaaggtgg tccagaacat caccatcgga
     1261 ccgacgctcg gccaggactc gatcaataag ggcatgctgg caactctcct cgcgaccctc
     1321 ttcgtggtcg tattcatgat cgtctattac cggtattcgg gcatcatcgc caatatcgcc
     1381 ctcggactca acctcatata tctgctcggt gtcttcagcg ccttcaaggc gacgatgacc
     1441 ctccccggca tagcgggtat cgcgctcacc atcggcatgg gcgttgattc caacgtcctc
     1501 atcttcgaga ggatacgcga agagctgcgg ctggggaaaa cggtccgggc ggcggtggac
     1561 ggtggctacg cgaaggcgtg ggtggcgata ttcgactccc acatcaccac gctcatcacg
     1621 gcggccgtcc tcttcatatt cggaacaggg ccgataaagg gtttcgcgat aaccctgatc
     1681 ttcggcatga tcataaatct ctttaccgcg gtgttcggat cgaagaccat cttcgactgg
     1741 atactcgtga aattcaaacc cagaacactg agtatatgag gcggcgccca tgatggagct
     1801 cattaaagat acgaaaatag atttcatctc ctttaggaag aaggcatttg tcatatcggc
     1861 gatactcgtg gctgtagggg tgttttcttt cattatgatc tctttcggta aggccaacct
     1921 cagcatcgac tttacggggg gcacgaacgt gcaggtgagg tttgccgaaa gggtcaatat
     1981 cggtgacctc aggtccgccc tggagaaggg gggtattggc gatgtgcaga tccaggaggt
     2041 cagcggaacg cgggatttct ttatcaagac gaagctttcg gacacggaaa aggagtccct
     2101 caccgcgaag ataacctcca tcctgtcgac gcagctgaag ggcgccaaat tcgaaatgct
     2161 ggggagtaac atggtcggtc ccggcgtagg ccagtcgctg aagaaatacg ccatcatcgc
     2221 catcgttctg gccctgctcg ggatcatcat ctacattgcc tggaggttta ccttcctgtc
     2281 gggcatcgcg gcgaccattg ccaccttcca cgatgttctg gccatagcgg gtgtcttcta
     2341 tcttctcaac aaggagttca atcttctcat catcacagct cttttgacca tagcgggata
     2401 ttccctccag gacacggtcg tcgtcttcga ccgcataagg gagaactcgg gcaagatgaa
     2461 atcccgcgac gattttggca gtatcataaa cgctagcatc aacgaggtcc tttccaggac
     2521 gatcgtcacc ggtatcagta cccttatcag tctagccgcc attatgatct ttggcgggga
     2581 agtgctcttc gatttcgcct ttgcgctttt tcttgggctc atcgtgggca cttactcgtc
     2641 gatcttcgtg gcgagcccga tcctctatgc atggcgccaa aagatccgtt aacggtaacc
     2701 ttgccgttag gacacccggg aaagatataa tgcggacgat ctgctgcagg tgc
//
LOCUS       DAOPOA010000280         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40032_length_2751_cov_3.094585, whole genome shotgun sequence.
ACCESSION   DAOPOA010000280 DAOPOA010000000
VERSION     DAOPOA010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40032_length_2751_cov_3.094585"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PKJ17_06620"
     CDS             <1..771
                     /locus_tag="PKJ17_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOD75682.1"
                     /translation="AKEALAGKVIDVVADSTDDLLAQIDGRTVETEKGKVVLHTKGAK
                     KIEIAMPFKFRFLAYVSDPNVAYILMMIGIYGILFEFYSPGTIFPGVIGGISLILALY
                     AFQAIPISFAGLALILLGIVFFILEVKIVSHGLLGIAGIVSVVIGSVMLVDLPGSPLA
                     ISFTTILIVAIISAIFVFGILSFAVKAQLSRVKTGSEGLLGEMGTARTKIAAGGRGKV
                     MVHGELWNAHSDEAIGEGEEIVVTAMEGLVIKVRKKGG"
     gene            776..1531
                     /locus_tag="PKJ17_06625"
     CDS             776..1531
                     /locus_tag="PKJ17_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOD75683.1"
                     /translation="MFSIPILFIVVIVVFFLFSAIKILNEYERGVIFRLGRVLGMPKG
                     PGLIILIPIIDRMVKVSLRTVVLDVPPQDVITRDNVSIKVNAVVYFRVVDPLKAIIDV
                     ENYLYATSQLSQTTLRSVLGQAELDDLLSHREQLNDRLQDILDTHTEPWGIKVSNVEV
                     KNVDLPQEMQRAIARQAEAERERRAKVISAEGEFQAATKLSEASEILSQNPMALQLRY
                     LQTLIEISTEKNSTIVFPLPIDLIKMFMDKLSK"
     gene            1551..2735
                     /locus_tag="PKJ17_06630"
     CDS             1551..2735
                     /locus_tag="PKJ17_06630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOD75684.1"
                     /translation="MKKLFVLFIALSLCLVGYTAQAEARNASVKKAPARQSREAEPPC
                     KSFIVVEAQGGKMIDGSNINEKRAPASITKLMVTYVVLGRLATGGIKLTDKVTISKEA
                     SKIGGSQVYLKEGEVFTLEELMKAMMVASGNDAAYAISEYVAGSKDEMVNLMNAQAKA
                     LGLADTEFHTVHGLPPAKGEKEDTTSCSDLAVLARELLKNPRVLEWTSIQRDTFRNGT
                     FVLTNTNKLLGKFAGTDGLKTGYYSQTGFNIVATAKRGDIRFIAVVMGSPTGKVRDSL
                     TMEKLQKAFSQYKMMNVVKKGEVIDQDIFLVDGKYRKMKGVTEKSFLYPMEVDKKGAI
                     MREVLLPERIKGEIKEGQRLGELVVKLNGQVIGKVNIVSPVYVPKANLFTRFIRRLGL
                     NI"
BASE COUNT          671 a          705 c          813 g          562 t
ORIGIN      
        1 gcgaaggagg ccctcgcagg gaaggtcatt gatgtcgtgg ccgattccac ggacgacctc
       61 ctggcgcaga tagacggaag gaccgtggag accgagaaag gaaaggtcgt gctgcacacg
      121 aagggcgcga agaagataga gatcgcgatg cccttcaagt tccggttcct ggcctacgtg
      181 agcgatccga acgttgcgta catactcatg atgatcggga tctacggcat ccttttcgag
      241 ttctacagcc cgggtacgat cttccccggc gttatcggtg gtatttctct cattctggcg
      301 ctctatgcct ttcaggcgat accgatcagt tttgccggac ttgcattgat cctcctgggt
      361 atcgtattct ttatcctgga ggtcaagata gtcagccacg gacttttggg catcgcgggc
      421 atcgtgtccg tcgtcatcgg gtcggtcatg ctcgtcgatc ttccgggcag cccgcttgcc
      481 atatctttca cgaccatcct cattgtcgcc atcatatcgg ccatattcgt tttcggtata
      541 ctctcctttg ccgtaaaggc gcagttgtcc agggtcaaga ccggctccga ggggcttctc
      601 ggggagatgg ggacggccag gacgaagatt gccgccggcg gcagggggaa ggtcatggtt
      661 catggcgaac tctggaatgc ccacagtgac gaggcgatcg gtgaaggcga ggagatcgtg
      721 gtcaccgcaa tggaagggct tgtcatcaag gtaaggaaaa aaggagggta agagtatgtt
      781 ttccattccg atcctgttta tcgtcgttat cgttgtcttc tttcttttca gtgcgataaa
      841 gatactcaac gagtatgaaa ggggcgtgat cttcaggctc ggcagagtgc tcggtatgcc
      901 gaagggaccg ggcctcatta tcctgatccc catcattgac cgcatggtca aagtgagcct
      961 gcggaccgtt gtgcttgacg tacctccgca ggatgtcata acccgcgaca acgtttccat
     1021 caaggtaaac gccgtcgtct acttccgtgt cgtcgatccc ctgaaggcca tcatcgacgt
     1081 ggagaactac ctctacgcga cgagccagct ttcccagacc accctgagaa gcgttctcgg
     1141 tcaggccgaa ctcgacgacc tgctttcaca cagggagcaa ttgaacgaca ggcttcagga
     1201 tattctcgat acccatacgg aaccttgggg cataaaagtc tccaacgtcg aggtgaagaa
     1261 tgtagatctg cctcaggaaa tgcagcgggc cattgcgcgc caggcggagg cggaaaggga
     1321 gagaagggcg aaggttatca gcgccgaagg tgagtttcag gcggccacaa agctttccga
     1381 ggcatcggag atcctctccc aaaacccgat ggccttgcag ttgaggtacc tgcagacatt
     1441 gatagagata tcgacggaga agaactcgac catagtcttt ccgttgccca tagacctcat
     1501 aaaaatgttt atggacaaat tgagcaagta aggcaaggaa tttggaggac atgaaaaaac
     1561 tgttcgtact gtttatcgcc ctatcgttgt gcctcgtcgg ttatacggcg caggccgagg
     1621 ccagaaacgc atcagtcaag aaagcgcccg cccggcagag cagggaggcc gaacccccct
     1681 gcaagtcctt tatcgtagtc gaggcccagg gcggcaagat gatagacggg agtaacatca
     1741 acgaaaagcg ggcccccgcg agcataacga agctcatggt gacctacgtc gtcctgggaa
     1801 ggcttgcgac cggggggatc aagctgacgg acaaggtaac catatcgaaa gaggcgtcga
     1861 agatcggggg cagccaggtc tatctcaagg agggcgaggt cttcaccctg gaggagctca
     1921 tgaaggcgat gatggtcgcc tccggcaacg acgctgccta cgccatcagc gagtatgttg
     1981 ccggcagcaa ggacgagatg gtgaacctca tgaacgcgca ggcgaaggcg ctcgggctcg
     2041 ctgacaccga gttccacacg gttcacggcc tgcccccggc aaagggggag aaagaggaca
     2101 cgacctcgtg cagcgacctc gcggttctgg cccgtgagct cctgaaaaac cccagggtcc
     2161 ttgagtggac gtccatacag agggatacgt tcaggaacgg cacgttcgtc ctgacaaaca
     2221 ccaacaagct cctcggcaag tttgccggca cggacggtct caagaccggg tattactccc
     2281 agacgggctt caacatcgtg gccaccgcca aacggggaga cataaggttc atcgcggtcg
     2341 tcatgggaag tcccacgggg aaggtgcggg acagtctcac catggaaaag ttgcaaaagg
     2401 ccttttccca gtataaaatg atgaacgttg tgaaaaaagg ggaagtgatc gatcaggata
     2461 tctttctcgt cgacggtaaa tacagaaaaa tgaaaggagt gacggagaag agtttccttt
     2521 acccgatgga agtcgataaa aagggtgcca tcatgcggga ggtcctcctg cccgagcgga
     2581 taaaaggaga gataaaggaa gggcagaggc ttggcgagct ggtggtcaaa ctgaacgggc
     2641 aggtgatcgg caaggttaac atcgtttccc ccgtttatgt gcccaaggcg aatctcttca
     2701 cgagattcat acggagactc ggactcaaca tttaaggcca tggatacgca a
//
LOCUS       DAOPOA010000281         2748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40077_length_2748_cov_4.167471, whole genome shotgun sequence.
ACCESSION   DAOPOA010000281 DAOPOA010000000
VERSION     DAOPOA010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40077_length_2748_cov_4.167471"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /gene="rpe"
                     /locus_tag="PKJ17_06635"
     CDS             complement(<1..555)
                     /gene="rpe"
                     /locus_tag="PKJ17_06635"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOD75685.1"
                     /translation="MKDILIAPSILSCDFLRLGQEIRDVEEAGADLIHVDVMDGHFVP
                     NITIGPMFVEALRRINRKPLDVHLMIENPAQYVKQFAEAGSDIVTIHAEADNHLFRTL
                     DVIKESGARAGISFNPATPLTLLRHVINIVDLVLIMTVNPGFGGQKYIPEMAGKIVEA
                     RKMIDKAGRYVDLEVDGGIKAENAA"
     gene            complement(569..1264)
                     /locus_tag="PKJ17_06640"
     CDS             complement(569..1264)
                     /locus_tag="PKJ17_06640"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOD75686.1"
                     /translation="MKLLKILFYVVAPVLVFGLSTYLTISLLLKFQQTTVCPDVRGKT
                     VEDARELLRKKDLALVVLRYERRNDVPYNHITVQKPDANISTKKGRVVYVIVSEGPEL
                     MKTPGFVGLTLEETQPVFSDKHLVLEKAVLVPHSKAGKVISQLPAEGTDILEYGKVTL
                     FVGTTPRSYYLMADTRNINYNELADELEAKGIKYRINYARTDQAPARGGIEYSILPRT
                     IFSSTEEIVINIY"
     gene            complement(1261..2487)
                     /locus_tag="PKJ17_06645"
     CDS             complement(1261..2487)
                     /locus_tag="PKJ17_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOD75687.1"
                     /translation="MRIIRDVESGRFLDESIDSAFSRSRLHEGERALIYQITSGVIRW
                     RGYLEIILSRYAKKPVEKDVRYLLWMTLYQVGFMKKAHYHVVKEAVEYARNEKGKFVA
                     GFVNAVLRRFVSDREDRSLSSAGAVRHAFPKWLVDRWTLRFGASETDGLLELLNREPE
                     FALRVHTLRLTVDRALEALTDGGLEVRRGRLSPSALTVDRLAPVLASSLFREGLVSVQ
                     GEASQLAGMAVAAAGGTTILDACTGSATKTKQVREMCPQARVISMDNKMKRLLLSSLG
                     DHIVCADALAPPFRAGYFDTILVDAPCSSLGIVRKHPEIKWRRSEEDISRFAAMQLSL
                     LRSLWDLVRPGGHVVYSVCSFEPEETTDVIRNLAKDKNFVLENPLPFLFNKDCFLSLP
                     HETALDGFFIARIRKL"
     gene            complement(2516..>2748)
                     /locus_tag="PKJ17_06650"
     CDS             complement(2516..>2748)
                     /locus_tag="PKJ17_06650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOD75688.1"
                     /translation="ADDGGANLSGNPLFLAGALRKLNAGVARNPMNDANPSTAPLFIV
                     NPFSAGGIRSLFSTHPPIEERIKRLEDMAYRR"
BASE COUNT          532 a          796 c          840 g          580 t
ORIGIN      
        1 ggcggcgttt tccgccttta taccgccgtc gacctcgagg tccacgtacc tgccggcctt
       61 atcgatcatc ttgcgcgcct ccacgatctt cccggccatc tcggggatgt acttctggcc
      121 tccgaaaccg gggttgacgg tcatgatgag aacgaggtcg acgatgttga tgacgtgcct
      181 gagcagggtg agcggtgtcg ccggattgaa ggatatgccc gcccgcgccc ccgactcctt
      241 gatgacgtca agggtccgga aaagatggtt gtccgcttcg gcgtgtatgg tgacgatgtc
      301 ggaaccggcc tcggcgaact gcttcacgta ttgtgccggg ttctctatca tgaggtggac
      361 atcgagaggt tttctgttga tcctgcgcag ggcctcgacg aacatggggc cgatggttat
      421 gttgggaacg aaatggccgt ccatgacgtc cacgtggatg aggtccgcgc ccgcttcctc
      481 aacatcccgg atctcctggc cgagacgcag gaaatcgcag gagaggatgg acggcgcgat
      541 gagtatgtct ttcataatgc ctccatggct agtagatgtt gatgacgatc tcctccgtgg
      601 aactgaatat cgttctgggg agtatcgaat actcgatgcc gccgcgcgca ggtgcctgat
      661 cggtcctggc gtaattgatc ctgtacttta tccctttcgc ctccagctcg tcggcgagct
      721 cgttgtagtt gatgttgcgc gtgtcggcca tgagataata ggacctcggt gtcgtgccga
      781 cgaaaagggt gaccttcccg tattcgagga tgtccgtgcc ttctgccggc agctgggaga
      841 tgaccttccc cgctttggag tggggaacga ggaccgcctt ttcgaggacg aggtgcttgt
      901 cgctgaacac cggctgggtc tcctcgagag tgagccccac gaaccccggg gtcttcatga
      961 gctccggtcc ttcggaaacg atgacgtaga ccaccctccc tttcttggtg gatatgttgg
     1021 cgtcaggttt ctgcacggtg atgtggttgt aaggcacatc attgcgccgt tcatacctga
     1081 ggaccaccag ggcgagatct tttttcctga gaagctcccg ggcgtcctca acggtcttcc
     1141 cccggacatc ggggcagacc gtggtttgct ggaacttgag gaggaggctt atcgtcaggt
     1201 atgtcgacag accgaagaca agaaccggtg ccacaacata gaataggatc ttcaagagct
     1261 tcatagtttc cttatccgtg ctataaagaa accgtccaga gccgtctcat gcggcaacga
     1321 aagaaaacaa tccttattaa acaggaacgg cagcggattt tcaagaacaa agttcttatc
     1381 ttttgccagg ttgcgaatga catccgtcgt ctcttcgggc tcgaaggagc agacgctgta
     1441 gaccacatgc cctcccggtc gcacgaggtc ccacagagac ctgagcaggg aaagctgcat
     1501 cgcggcgaac cgggagatgt cttcttcgct ccgccgccac ttgatctcgg ggtgcttgcg
     1561 gacgatgccc agcgatgagc agggcgcgtc gacaaggatg gtgtcgaagt atccggccct
     1621 gaaaggcggg gcaagggcgt cggcacagac gatatgatcg ccgagcgacg acagaaggag
     1681 gcgcttcatc ttgttgtcca tggagatgac gcgagcctgg ggacacatct ccctcacctg
     1741 cttcgtcttc gtcgccgaac cggtgcacgc gtcgaggatc gtggtgccgc ccgctgccgc
     1801 gaccgccatg cccgcgagct gggacgcctc gccctgaacg ctcacgagcc cctccctgaa
     1861 aagggagctc gcgagcaccg gggcgagcct gtcgacggtg agggccgagg gcgaaagacg
     1921 gccacggcga acctcgagcc cgccatccgt cagggcttcg agcgccctgt cgacggtcag
     1981 tctgagcgtg tgcaccctga gcgcgaactc gggttccctg ttcaagagct caaggagccc
     2041 gtccgtctca ctggccccga acctcagcgt ccagcggtcg acgagccatt tcgggaaggc
     2101 gtgccgcacc gccccggcag aggagagaga gcggtcctcc ctgtcgctca cgaacctcct
     2161 cagcacggcg ttcacgaacc cggccacgaa ctttcctttt tcattcctgg cgtattccac
     2221 ggcctcctta acgacatggt aatgggcctt cttcatgaac ccgacctggt agagcgtcat
     2281 ccagaggagg tatcggacgt ccttttcgac aggttttttg gcgtaccggg agaggatgat
     2341 ctcgaggtat ccccgccacc gtatcacccc cgaggttatc tggtatatca gcgcccgctc
     2401 accctcgtgc aggcgtgagc gcgagaaggc cgaatcgatg gattcatcga ggaacctgcc
     2461 ggattcaaca tcccgaatga tgcggatcga aatggccctg aggagatgtc cgaagttatc
     2521 gccgataggc catgtcctcc agtctcttga tcctctcttc gataggaggg tgggtgctga
     2581 acagggagcg aatgcctccc gcgctgaagg ggttcactat gaagagcgga gccgtggagg
     2641 ggttcgcgtc gttcatggga ttgcgcgcca cgccggcatt gagcttgcgc agggccccgg
     2701 cgagaaagag gggatttccc gacaggttcg ctccgccgtc atcggcca
//
LOCUS       DAOPOA010000282         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40096_length_2747_cov_2.973997, whole genome shotgun sequence.
ACCESSION   DAOPOA010000282 DAOPOA010000000
VERSION     DAOPOA010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40096_length_2747_cov_2.973997"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PKJ17_06655"
     CDS             <1..435
                     /locus_tag="PKJ17_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75689.1"
                     /translation="RSTFYGRQEKGAGKKKEAPKDRWQQGRVRDALDRLFKGDKGTPE
                     HDKLFARLHNAYGSEAFPKQAERYMAKYGLPDDGPTLILFLDLKDADVCNSTLDKLRE
                     LYASLPPRQKEDARRKISIAAMAHKIKEVRIKAQEVIEELEE"
     gene            complement(580..771)
                     /locus_tag="PKJ17_06660"
     CDS             complement(580..771)
                     /locus_tag="PKJ17_06660"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOD75690.1"
                     /translation="MECTIEKNKAACACTYDPCPRKGKCCECVAYHRARKQLPGCFFS
                     KEGEASYDRSYRRFVMENS"
     gene            1017..1700
                     /locus_tag="PKJ17_06665"
     CDS             1017..1700
                     /locus_tag="PKJ17_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75691.1"
                     /translation="MTTTIKIGRLVRCAIVKDLADRDVYLVIVTGTDLLAYLPKRFAG
                     REYRQGEETVAAVFMMGKGKIFLSQRSPQYFRRITEEALSPIIRDGRVRVRRAASVIN
                     GPFAKVSVEGLGDVDPLSASLPYLEGVKAYTADTITIVRYSTDMEKYIVNSLVPAPPD
                     KVVEVVYSRGLREAIVRVDPRYCGFFVGKGGTNVATAAKLLNVRIMIKSAGEEAGAKE
                     GGICADGRS"
     gene            1687..2019
                     /locus_tag="PKJ17_06670"
     CDS             1687..2019
                     /locus_tag="PKJ17_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75692.1"
                     /translation="MGGVDRKGLGVKGLIIVVGSCITCSAATVAAYDLFFAQKIVAVD
                     LASSIASQKEEYTAGKITADQLVENIEALLKRMEKRKRNEVLILEEAVAGDISHRSLG
                     NDGGGEEE"
     gene            2191..2412
                     /locus_tag="PKJ17_06675"
     CDS             2191..2412
                     /locus_tag="PKJ17_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75693.1"
                     /translation="MDEKNGKCPHCGGGNIIVGVRVDQTADAGRIGLAYKTKFVLGGT
                     EPFYADVCDDCGTITRIYVKETGKNWYRK"
     gene            complement(2539..>2747)
                     /locus_tag="PKJ17_06680"
     CDS             complement(2539..>2747)
                     /locus_tag="PKJ17_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="proline iminopeptidase"
                     /protein_id="HOD75694.1"
                     /translation="RVDRLREITVPTLFTAGRYDEATPGATTWYSSHLPGSTVRIFQK
                     SSHMAMFEEQEEYMRTLREFMRQF"
BASE COUNT          656 a          704 c          829 g          558 t
ORIGIN      
        1 aggagcacct tttacggcag gcaggaaaag ggggccggaa aaaagaagga agctccgaag
       61 gaccgctggc agcagggaag ggtaagggat gcgctggaca ggcttttcaa gggtgacaag
      121 ggaacccccg agcacgacaa gctctttgcc aggctgcaca acgcctacgg gtcggaggcc
      181 tttccgaagc aggccgagag atacatggca aagtatggcc tgcccgacga cgggcccacg
      241 ctcattctct tcctggacct gaaggacgca gatgtgtgca actccaccct cgacaagctc
      301 cgtgaactct acgcgtcatt gccgccgcgt cagaaggagg acgccaggcg gaagatctcc
      361 atcgccgcca tggcccacaa gataaaagag gtccgcataa aggcccagga agtgatcgag
      421 gaactggaag aatagaaaag acggtgttaa gttttaagtg ttaggtttta agttaaaagc
      481 cttaggacac tttcccgcat tccgttgaac gtacaaagat cctctcaagc gaaaggtctt
      541 tcacgtaaaa cttaaaacct aacacttaaa actgtttttt caactatttt ccatcacgaa
      601 ccgcctgtat gaccggtcgt aggaggcctc gccttccttt gaaaagaagc agccgggcag
      661 ctgtttcctg gcccggtggt aggcgacgca ttcgcagcat tttcctttcc gggggcaggg
      721 atcgtaggta caggcgcagg cggccttgtt cttctctatc gtgcattcca tctcattcac
      781 ctcgcgcgga tatttgacga acagtatata gggaagaccc cgtagtttca aggttacgaa
      841 aagcgggtaa tgggtcacgg ggtatgggaa gaaccctgga atgtttgccg gtattgatgc
      901 atttccccaa cacccatgac ctattaccca taacccgctt ttgctttttt tcgggtcctt
      961 ttttctctcc cggaagtaat acacctgtgc agtgagtata tctcaggggg gagatcatga
     1021 cgacgacgat caagataggt cgccttgtgc gctgcgctat cgtgaaggac cttgccgaca
     1081 gggacgtcta ccttgtcatt gtgacgggga cggacctctt ggcctacctt ccgaagaggt
     1141 tcgcgggcag ggaatacagg cagggggagg agacggtggc cgcggtgttc atgatgggaa
     1201 aggggaagat atttctgagc cagaggtcac cgcagtactt ccgccggatc acggaggagg
     1261 cgctgtcgcc catcatacgc gacggcaggg ttcgggtgcg aagggcggcg agcgtgatca
     1321 acggcccctt tgccaaggtt tccgtcgagg gcctcggaga cgtggatccg ctctccgcgt
     1381 cgcttcccta cctggagggg gtgaaggcgt acaccgcgga taccatcacc attgtccggt
     1441 acagcacgga catggagaaa tacatagtca attcccttgt tcccgcgccg ccggataagg
     1501 tggtggaggt ggtctattca cggggcctga gagaagccat cgtcagggtc gaccccaggt
     1561 attgcggctt ctttgtcggg aaagggggta cgaacgtggc cactgccgcc aagcttctca
     1621 acgtcaggat catgataaag agcgccgggg aggaagcggg agcaaaggag ggaggcatct
     1681 gtgcggatgg gcggagttga cagaaagggc ctcggcgtga aagggctcat catcgtggta
     1741 ggctcgtgca ttacctgctc agccgccacc gttgccgcct acgacctctt cttcgcgcag
     1801 aagatagtcg ccgtggacct cgcctcctcc atcgcgtcgc agaaagaaga atacactgcc
     1861 ggcaagatca cggcagacca actcgtcgag aacatcgagg ctctcctgaa acggatggaa
     1921 aagagaaaga ggaacgaggt cctcatcctc gaggaggcgg tcgccggcga tataagccac
     1981 cgtagtctgg ggaacgacgg agggggagag gaggaatgat gcgagagtgc ccggacgaat
     2041 gggaagaccg agacgaaacg atcgctatcc tcaatgatga ggagctttaa agagtattcc
     2101 cgctttgcag agcttgacat gcctcaaccg acgttctagg ctgtttcaac tccccgcatc
     2161 aggggactgg acgaaaagag cggaggcgct atggacgaga agaacggcaa atgcccccat
     2221 tgcgggggcg gcaacatcat cgttggcgta agggtcgatc agacggcgga cgcgggcagg
     2281 atagggctcg cctacaagac caaattcgtg ctgggaggca cggaaccttt ctatgccgac
     2341 gtctgcgacg actgtggcac catcacccgg atctacgtga aggaaacggg gaagaactgg
     2401 tataggaaat gaagaccgtt ttaagtttta agttattagg ttttaagtga aagccttcgt
     2461 tcaacggatg ctcgcacgtt caacgtgacc cgcggagcgg tttcccggtc tttgacctaa
     2521 aacgtaataa cttaaaactt aaaactgtct cataaactcc cgtaatgtcc tcatgtactc
     2581 ttcctgctct tcgaacattg ccatgtggga acttttctgg aagatccgca cggtggaacc
     2641 cgggagatgg gagctgtacc aggttgtagc gccgggggtt gcctcgtcgt accggcccgc
     2701 ggtgaagagg gtgggcaccg tgatctcccg gagccggtcc accctgt
//
LOCUS       DAOPOA010000283         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40122_length_2746_cov_2.467856, whole genome shotgun sequence.
ACCESSION   DAOPOA010000283 DAOPOA010000000
VERSION     DAOPOA010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40122_length_2746_cov_2.467856"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(510..>690)
                     /locus_tag="PKJ17_06685"
     CDS             complement(510..>690)
                     /locus_tag="PKJ17_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75695.1"
                     /translation="GSHFTYNASAIFGYHFSPMVSAWLGYRILGIDYEDGHGPDKFKY
                     DVTMQGPIVGIGFRF"
     assembly_gap    691..700
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<701..1335)
                     /locus_tag="PKJ17_06690"
     CDS             complement(<701..1335)
                     /locus_tag="PKJ17_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75696.1"
                     /translation="MISGKFGLITMAVLLSLLLPPAGHGQSTLDPKPAPERWEFFVAP
                     YFWMAGLDGKTTVNGVESNVNFSFSDIWESLDIGSQVHVEGWRGKWGIFLDATYLKLS
                     NDGSGVSPAYGPVDIDVRLEEWIVEGGGFYRFLTRPIDRDRRVSVDALAGLRYISLYG
                     KLSAFVPLVGLDTSPSGTKDWVDPFLGLRLMADITKKLAIALKVVGLMNIQ"
     gene            complement(1460..>2746)
                     /locus_tag="PKJ17_06695"
     CDS             complement(1460..>2746)
                     /locus_tag="PKJ17_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75697.1"
                     /translation="GIEEVRGTLKRKRYLRIKGIHPGDTLYVYVEAVSGNLRPIVRLQ
                     DFGGKALAIANFKGESTAAALKYRFPRYMRNCMVIVSGTLRDKTVTTGDYRALIGVNA
                     PEILEGKGEAGGNPVLEKPVPVKIGIKMQQISNVDQRSENFGVVATLMMKWEDPLLAF
                     RPDAAQTRAQVYTRESFTRKLSEEGRMWPDYSIYNQQGNRWIQNSVAVVFADGRVLYL
                     ERFTVTLQAPDFDFRQFPFDKQSFFIRVDSIFPLWFFAFEDLEGFSEAGNQLGEEEWI
                     VTGVDTSVDRAELAERPVSRFTFHFRARRHLTYYAFRIFLPLLIIIVVSWIIFFLRDY
                     TKRVDASGANLLLFIAFNFAISSDLPRLGYMTFLDMLLAAVFVVTALMLILAVLVRRL
                     VSDGRDLLVSRIDRYVIWFYPFAYIVGLLIVILVYR"
BASE COUNT          597 a          791 c          736 g          612 t
ORIGIN      
        1 gggagtactt tgtttctgcc gcgggggcta tatcaaggtt agcggacccc agcacgataa
       61 gcaatactga caagaaaagg gtcaatcgtc tcatcccgtc ctcctatctt cccgtcatac
      121 acctaacggt cagcgatgtc tcggccatgt tttacaaacc tcttcgatgt ttcaggatct
      181 tcggtctcgt ccttacgaaa cccgctttca ccctccctgg tatctgccgg gttccaggtt
      241 accaacagat gtgaacgtcc gttgcccatt tctcatttca gtgtccccct gtgtttgtaa
      301 gattgtgttc ttcttgatga acatgcttca tcgggccact ttttcgggat ggctggccga
      361 cacatcacgg cgcacaggcg ctggcccttc tcgaaactcg agatgcgcat gaactcattg
      421 tctgcgtgga atccttggtt ttttctgcac aaagcccaaa cctgtctttg cctgccccgt
      481 tttcgcggaa tttgagcgta ggccgggtgt cagaaccgga acccgatgcc tacgatcggt
      541 ccctgcatgg tgacgtcgta cttgaacttg tccggcccgt gcccgtcctc gtagtcgatg
      601 cccaggatgc ggtagcccag ccaggcgctg accatggggg aaaagtggta accgaatatg
      661 gccgacgcgt tgtacgtgaa gtgggagccg nnnnnnnnnn tactggatgt tcatgagacc
      721 cacgaccttg agcgcgatgg cgagcttctt cgtgatgtcg gccatgagcc tgaggcccag
      781 gaaggggtcc acccaatcct tggtgccgct cggagaggta tcgaggccca cgagaggaac
      841 aaaggcggac aatttcccgt aaagggatat ataacgcaga cccgcgagcg catctacgct
      901 gaccctgcgg tcccggtcga tgggacgggt gaggaaccga tagaaacctc ccccctccac
      961 gatccattcc tccagccgca cgtcgatgtc aacagggccg tacgcggggc tcactcccga
     1021 tccgtcgttg gaaagcttca ggtacgtggc atcgagaaag atgccccatt tacccctcca
     1081 gccttccacg tgtacctggg acccaatgtc aaggctctcc cagatatcgc tgaaagagaa
     1141 attgacgttg gattcgacgc cgttcacggt cgtcttgcca tcgaggcctg ccatccagaa
     1201 gtacggggcc acgaagaatt cccacctctc aggtgcgggc ttcgggtcca gagtactctg
     1261 gccgtgaccg gcgggaggga gcaatagaga gagcagtacc gccatcgtga tgaggccgaa
     1321 ttttccggat atcattctcg cacctcctta ttggtttatc ctgtcctgtc gatgcgctga
     1381 tttttgctgg aaagtcgcca agactatctc gtcgggtcga cctcgttgat tgccgaactc
     1441 tttttagtgg tgatctcgac tatctgtaca cgaggatcac gatgagaagc cccacaatgt
     1501 aagcgaaagg gtagaaccag atcacgtagc ggtctatcct gctcacgagc aggtctcttc
     1561 cgtcactgac caggcgtcgt acgagtacgg ccagtatgag catgagcgcc gtcaccacga
     1621 agacggcggc cagcaacatg tcgagaaacg tcatgtatcc gagtcgcgga aggtcgctgg
     1681 agatcgcgaa gttaaaagcg atgaacaaca ggaggttggc ccccgatgcg tccacacgct
     1741 tggtatagtc acgcaggaag aagatgatcc atgacacgac gatgatgatc agcaggggaa
     1801 gaaagatacg aaaggcatag tacgtgaggt ggcgccgggc ccggaagtga aaggtgaagc
     1861 gggataccgg ccgctccgcc agttccgccc tgtctacgga agtgtcgaca ccggtaacta
     1921 tccactcttc ctcccccagt tgattgccgg cctcgctgaa accttcaagg tcctcaaaag
     1981 caaagaacca taagggaaag atggaatcaa ccctgatgaa gaacgactgc ttgtcgaagg
     2041 ggaactggcg gaagtcaaaa tcgggcgcct ggagcgtcac ggtaaaacgt tccaggtaaa
     2101 gcacccttcc atcggcaaag accacggcga cgctattctg gatccatcgg ttaccctgct
     2161 ggttgtagat ggaataatca ggccacatcc tgccctcttc gctcaacttc cgcgtgaagg
     2221 actccctggt gtaaacttga gctcgtgtct gtgcggcatc gggtctgaat gccaaaaggg
     2281 ggtcctccca tttcatcatg agcgtggcaa cgaccccgaa gttctccgac ctctggtcca
     2341 cattggatat ctgctgcatc ttgatgccga tcttcacggg cacgggcttc tccaggacag
     2401 ggtttccgcc cgcttcgccc tttccttcca ggatctcggg ggcattcacc ccgatcagcg
     2461 cccgataatc gcccgtcgta accgtcttgt cccgaagcgt tcccgatact atgaccatgc
     2521 aattccgcat gtaccgggga aacctgtact tcagtgcggc ggccgtgctc tcacccttga
     2581 aattggcaat tgcaagggcc tttcccccga aatcctgaag cctgactatg ggtctgaggt
     2641 tgcctgagac agcctccaca taaacgtaca gggtatcccc gggatggatg cctttgatcc
     2701 tcagatagcg ctttcttttg agggttcccc tcacctcctc tatccc
//
LOCUS       DAOPOA010000284         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40410_length_2731_cov_4.220105, whole genome shotgun sequence.
ACCESSION   DAOPOA010000284 DAOPOA010000000
VERSION     DAOPOA010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40410_length_2731_cov_4.220105"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKJ17_06700"
     CDS             complement(<1..444)
                     /locus_tag="PKJ17_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75698.1"
                     /translation="MLLINPPVAKPCEPPAGLARLAGALGDAGHPCKVLDMNLEGMLR
                     LISNAVPGDDRWSQRAARNLSRNLDALRDERTYRNFDRYKRCVNDVNRSFFFAGGENT
                     RLSLADYEDKRLSPIRSEDLMEAARHPGDSPFYEHFAGRLREELGR"
     gene            complement(483..1343)
                     /locus_tag="PKJ17_06705"
     CDS             complement(483..1343)
                     /locus_tag="PKJ17_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75699.1"
                     /translation="MKKLSVALLLIFCAAAVLAGAGEVSAAVQAGYIFDIEGTAQLRS
                     ADGKTITLKRSEHILYAVKEGDRISVRKGRVVVASLKESKGYEIGDNSEGLAKPGRMV
                     AVRGKISELTGLYAPGGTSSGSMGGFVVRGVRPCIKAISPVSTTILDPAPVLSWENRC
                     SGDRKVTVKIISGDTVVYSAESEGSSLKVPDKVLAYGNEYRWIVDSGKVNNVSGGVFS
                     LPSEAEAEELGRKIAAFKERKEDLSFRLSYVFFLVDNNLNDLARAEIKTLKADFPENE
                     HLKKMEESIR"
     gene            complement(1402..>2731)
                     /locus_tag="PKJ17_06710"
     CDS             complement(1402..>2731)
                     /locus_tag="PKJ17_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOD75700.1"
                     /translation="IADAALTDGGLTGNDGVIRKQVLDRGVSPSFASVLYRLSTGRQY
                     GRGPVFLNYALTGSIPFYRLDDVMEGRIDRGAFSGKTVILGYISGYEDVHATPLKRDL
                     FPMDTVKRDASPSQRGGGRMPGPVIHGIIAETMLTGTSLTEAPLGANIAILAFLAASS
                     LAAISLMRPLPAIAAIVGLTALFFAVNLLLFAAGSYVHIFPQALVPFVVAILVYPYRY
                     LVEERARRKVEKVFGYYMDRNVLDRLIETDPEALLAGEARDVSILFLDIRNFTVLSTQ
                     REARQVVRFLNSFFGAVTERIQERGGFVNKFIGDGLLAFFMTGQNPAADAVSAALNIL
                     DETRRLNDEGRFREFIGDWQVSVGIGIHHGEVILGNIGSHRKMDFTIIGEHVNIASRI
                     EGLTKEAGAALLVSSDARAAAADVFPWREAGEFALKGVDRPISLYTVGET"
BASE COUNT          525 a          846 c          804 g          556 t
ORIGIN      
        1 gcgccccaac tcctccctga gacgtcctgc gaagtgctcg tagaaagggc tgtccccggg
       61 gtgacgagcc gcctccatga ggtcctcgct tctgatcggt gacaaacgtt tgtcctcata
      121 atcggcgagg ctgagccttg tgttctctcc accggcaaag aaaaaagacc ggttgacgtc
      181 gttgacgcat ctcttgtagc ggtcgaagtt gcggtatgtc ctttcgtccc gaagggcatc
      241 gaggtttcgg gaaaggtttc gcgcggccct ctgcgaccac ctgtcgtcac cggggaccgc
      301 attggagata agccggagca tgccttcaag gttcatgtcg aggaccttgc agggatggcc
      361 ggcgtcgccc aaggcgccgg cgagacgcgc gaggcccgcg ggaggttcgc agggtttggc
      421 tacaggggga ttgataagga gcacgttctt tctttcaggc aggacgggaa cgacattgag
      481 ctctacctga tgctttcctc catctttttc aaatgttcgt tctcggggaa atcagctttc
      541 agggtcttga tctcggcacg cgccagatcg ttcaggttgt tgtcaacgag gaagaatacg
      601 taggagagcc tgaaggaaag atcctctttg cgctctttga aggcggctat cttccggccg
      661 agctcctcag cctccgcctc ggacggcagg gagaagacgc ctcccgaaac gttgttcact
      721 ttgccggaat ccactatcca ccggtactca ttcccatagg ccagcacctt atcgggtacc
      781 ttcagggagc ttccctcgct ctccgcgctg tacacgacgg tgtcgccgga gattatcttc
      841 accgtcacct tcctgtcgcc ggaacatcta ttctcccagg agagcaccgg cgccgggtcg
      901 agtatggtgg tgctcaccgg agatatcgct ttgatacagg gccggacgcc gcgcacgaca
      961 aagcctccca tggaccccga tgaggtgcct cccggggcat agaggcccgt gagctccgag
     1021 atctttcccc tgacggccac catcctgccc ggtttcgcca atccttccga gttgtcgccg
     1081 atctcgtagc ccttgctctc cttcagcgac gcgacgacca ccctgccctt tcgcacgctg
     1141 atcctgtccc cctccttgac ggcatagagg atgtgttcgc tccgtttgag ggtgatcgtc
     1201 tttccgtcag cgctcctgag ctgcgctgtc ccctcaatgt cgaagatgta ccctgcctgg
     1261 acggccgcgc tcacctcgcc ggccccggca aggacagccg cggcgcagaa gatcaaaaga
     1321 agggccacag atagcttttt catgttcttc tccttctcct ggaaatatcc tctcctgttc
     1381 cccgcatgta cctacatcac atcacgtctc tccgacggtg taaagtgaaa tggggcggtc
     1441 cacccccttg agggcaaatt cccccgcctc cctccacggg aagacgtccg ctgcggcggc
     1501 gcgggcgtcg ctgctcacga gaagcgccgc gcccgcctcc tttgtgaggc cctctatgcg
     1561 ggaggcgatg ttcacgtgct cgccgatgat ggtgaagtcc atcttcctgt gagagcctat
     1621 gttgccgagg atgacctcgc catgatgtat gccgatgccg acgctcacct gccaatcgcc
     1681 gatgaactcc ctgaaccggc cctcgtcgtt gaggcgcctc gtctcgtcga ggatattaag
     1741 ggccgccgac accgcgtcgg cggcagggtt ctgaccggtc atgaaaaagg ccagcaggcc
     1801 gtcaccgatg aacttgttca cgaaaccgcc ccgttcctgt atcctttcgg tgacggcgcc
     1861 gaagaaggaa ttgaggaaac gcaccacctg gcgtgcctcc ctctgcgtcg agaggaccgt
     1921 gaagttccgg atatccagga acagtatgga aacatcccgc gcctcgccgg caagaagggc
     1981 ctccgggtcc gtctcgatga gcctgtcgag aacattcctg tccatgtagt atccgaagac
     2041 cttctccacc ttccgccgcg ccctttcctc gacaagatac cggtaaggat acacgaggat
     2101 ggcaacgacg aagggcacca gcgcctgggg gaaaatgtgg acgtagctcc ccgcggcaaa
     2161 gaggagaagg ttgacggcaa agaacagggc ggtgaggccg acaatcgcgg cgatcgccgg
     2221 aagaggcctc atgagggata tggccgcgag cgacgaggct gccagaaaag cgagtatggc
     2281 gatgttggcg ccaaggggcg cctccgtcag agaggtgccc gtcaacatgg tctccgctat
     2341 tatgccgtgg atcacgggac cgggcattct gccgcctccg cgctgcgaag gcgaagcgtc
     2401 tcgtttcacg gtgtccatgg gaaaaaggtc ccgcttgagg ggcgtcgcgt ggacgtcctc
     2461 gtatccgctg atataaccca gaatgaccgt ctttcctgaa aaggcccccc tgtcgatcct
     2521 gccttccatg acgtcgtcaa ggcggtagaa gggaatggag cctgtcagcg cgtaattcag
     2581 aaagaccggg ccccggccgt attgccgtcc cgtggacagg cggtagagaa ctgaagcaaa
     2641 ggacgggctg acgcctctgt cgaggacctg cttccggata acaccgtcgt tcccggtgag
     2701 cccgccatcc gtaagggcgg cgtcggcaat g
//
LOCUS       DAOPOA010000285         2730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40443_length_2730_cov_2.569720, whole genome shotgun sequence.
ACCESSION   DAOPOA010000285 DAOPOA010000000
VERSION     DAOPOA010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40443_length_2730_cov_2.569720"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PKJ17_06715"
     CDS             complement(<1..945)
                     /locus_tag="PKJ17_06715"
                     /inference="COORDINATES: protein motif:HMM:NF022839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcNAc-transferase family protein"
                     /protein_id="HOD75701.1"
                     /translation="MESIFISIASYRDNELGRTIRDCTDKAAHPERLVFGIHWQHDNH
                     EFFEQFYDRRCRVIDTDYRESRGACWARHEAQKLYAGEDFLLQIDSHMRFVRDWDRIC
                     VDGVRKLQERGNRRVIISNLMPSYDPDDDGNLPLAYLTYRMGSFNQDGAIALKASVVQ
                     KPPPEPFSPGFSISAAFVFSIGDLYREVPIDPDLYFDGEEISYAVRAYTHGYDIYHCH
                     HPIVYHYYTRKTDRKHWDDHGNWGERSLMAKARMRTLLMRTGEAADLGPYGLGTMRSI
                     EDYERHSGVNFRERTVKDPADEGSFPVTRDWIRSKLAGG"
     gene            complement(946..1350)
                     /locus_tag="PKJ17_06720"
     CDS             complement(946..1350)
                     /locus_tag="PKJ17_06720"
                     /inference="COORDINATES: protein motif:HMM:NF025047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HOD75702.1"
                     /translation="MFQNTMGGGMNLGFPDVCLTPTPVGPIPIPYPNISTGATTNPAT
                     AALTVLCDGMPALNQMSMGTISNGDNAGVNLGVASGLVMGPTEFILGSFTVLKGGTPA
                     QRMTSITGHNGLSMNGPGVSLAPSQVAVLVLG"
     gene            1742..>2730
                     /locus_tag="PKJ17_06725"
     CDS             1742..>2730
                     /locus_tag="PKJ17_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOD75703.1"
                     /translation="MNSRKSSSTLRSIMRLLCGGSFLISRDLRIEWVGSVSRRWSGGK
                     DAVTLEGKHCYSVIMGRRKPCESCPVARCYESGRMEEMEIKIGKGADAEHFVLTASPL
                     SSEDGGLMGVVEIIRDVTAFRRTEEDLRQLGEFNYEIVENAPVAIFTINKKGEFSSVN
                     PALVTLSGFDSMAKAKEKLLGFNWIKNPYTKKCGMSNHIKEGLKGKPFQLWDFPFIDY
                     WGVPQYIYFKGVPIKDKDGRVEGLLCIIEETTERVRARDQLMQEAKMSMIGRLTTGIA
                     HELNNPLATITANAELALESLENLEGGKFSRRDLEEFKESLRVIEEQAFRCTKT"
BASE COUNT          571 a          749 c          858 g          552 t
ORIGIN      
        1 tcccccggcc agcttggagc gtatccagtc cctggtgaca gggaaagagc cctcatcagc
       61 ggggtctttc acggtgcgtt cccggaaatt gacgccggaa tgccgctcgt agtcctcaat
      121 ggagcgcata gtgccgaggc cgtaggggcc aaggtcggcg gcctcaccgg tccgcatgag
      181 aagggtccgc atccgggcct ttgccatcaa ggacctttcc ccccagttgc cgtggtcgtc
      241 ccagtgcttg cggtccgtct tccttgtata ataatggtag acgatggggt ggtggcagtg
      301 atagatatcg tagccatggg tgtaggccct caccgcatag gagatctcct ccccgtcgaa
      361 atagaggtcg gggtcgatgg ggacctcccg gtagaggtcg ccgatggaga agacaaaggc
      421 ggcggagatg gagaaaccgg ggctgaaagg ctcgggcggt ggtttttgga cgacggacgc
      481 cttgagcgcg atggcgccat cctggttgaa ggagcccatc ctgtacgtaa ggtaggccag
      541 ggggaggttc ccgtcgtcat cggggtcgta ggagggcata aggttgctga tgatgacccg
      601 cctgttcccc ctttcctgga gtttccttac accgtcgacg cagatcctgt cccaatcgcg
      661 cacgaaccgc atgtggctgt cgatctggag gaggaaatcc tctccggcat agagcttctg
      721 cgcctcatgg cgcgcccagc aggccccccg cgattccctg tagtccgtgt cgataaccct
      781 gcagcggcgg tcatagaact gctcgaagaa ctcgtggttg tcgtgctgcc agtgaatgcc
      841 gaacacgagc cgttcggggt gggccgcctt gtcggtgcaa tcgcgtatcg tcctgcccaa
      901 ctcgttgtcc cggtagctgg cgatgctgat gaagatgctt tccatctacc ccaggacaag
      961 caccgcgacc tggctcgggg cgagactgac gccgggcccg ttcatggaca ggccattatg
     1021 ccccgtaatg cttgtcatcc tctgcgccgg tgtccctcct ttcaggacgg tgaaactccc
     1081 gaggatgaac tccgtcggtc ccatgacgag ccccgaagcg actccgaggt tcacaccggc
     1141 attgtccccg ttgcttatcg tccccatcga catctggttg agcgcgggca tgccgtcaca
     1201 tagcacggtg agcgcggctg ttgccgggtt tgtcgttgct cccgttgaga tattgggata
     1261 ggggatgggt atcggcccca ccggtgtcgg cgtgagacat acgtcgggaa agcccaggtt
     1321 catcccgccc cccatggtat tctgaaacat agtccctcct ttcaggccag atgtgccgac
     1381 ggaacagtca acaacagacc cggaccgggt aggaactatc tcaataagat aagggacata
     1441 gcgcacattt tcgtttagat gatatgggga tgtggtgata aagtcaaggg aaaacaggta
     1501 aagacaggta aagaccgtaa agaccgcttg agtggcttga atcgcttgag tggcttgaaa
     1561 gtagaagaat caggcttgcc ggtacaccca ggctggagtg tggacgcaag gcctttcggt
     1621 ccgctccgtc ctctaactct caagccattc aagcgattca agccactcaa gcggtcgcag
     1681 cccccggctg cgacctttac gtctggcacc ttttttgcta tcctatctcg tcatggccga
     1741 gatgaattca cggaagagct cctccaccct gaggtcgatc atgcgccttc tgtgcggggg
     1801 gtccttcctg atcagccggg acctcaggat cgaatgggtg gggtccgtga gccgccggtg
     1861 gtcgggaggg aaggatgccg taacgcttga aggcaagcac tgctattccg tcatcatggg
     1921 gaggcgcaag ccctgcgaga gctgtcccgt cgcgaggtgt tacgaaagcg gcaggatgga
     1981 ggagatggag ataaagatcg gcaagggggc cgacgccgaa cactttgtcc tcaccgcgtc
     2041 ccccttgagc agcgaggacg gcggtctcat gggggttgtc gagatcatac gggacgtcac
     2101 ggccttccgg aggaccgagg aggaccttcg ccagctcggg gagttcaatt atgagatcgt
     2161 ggagaacgcc ccggtggcga tctttaccat taataagaag ggggagtttt caagcgtcaa
     2221 cccggcgctc gtcaccctgt cggggttcga cagcatggcg aaggcgaaag agaagctcct
     2281 cgggttcaat tggataaaga acccctacac gaagaaatgc ggcatgtcta accatataaa
     2341 agagggtctc aagggcaagc cctttcagct ctgggatttc ccctttatcg attactgggg
     2401 ggtgccgcag tacatctact tcaagggtgt tcccatcaag gacaaggatg gccgtgtgga
     2461 aggcctcctg tgcatcatcg aggagacgac ggagcgcgtc agggcgcggg accagttgat
     2521 gcaggaagcc aagatgtcca tgatagggag gcttacgacg ggcatcgccc acgagctcaa
     2581 caatcccctc gccaccataa cggccaacgc ggagcttgct ctggaatccc ttgagaacct
     2641 cgagggggga aagttcagca ggagggacct ggaggagttc aaggaaagtc tgagggtgat
     2701 cgaggagcag gcgttcaggt gcaccaagac
//
LOCUS       DAOPOA010000286         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40466_length_2728_cov_3.033296, whole genome shotgun sequence.
ACCESSION   DAOPOA010000286 DAOPOA010000000
VERSION     DAOPOA010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40466_length_2728_cov_3.033296"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1488
                     /locus_tag="PKJ17_06730"
     CDS             280..1488
                     /locus_tag="PKJ17_06730"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOD75704.1"
                     /translation="MSIGFEEALRLTFEAIGPLPAESVGLLECVGRVAASDLFALVDS
                     PSSDVSRKDGYAVVYGEVKDATAAVPVRLSLAGSTSAGAAKNIVLTEGTTVRVLTGAR
                     VPAGADAVVADEYIRADGRDILIESPPDEPDRNILARGRDVRAGQRLIEAGRRISPAA
                     AGFLAAAGLGRVPVFSKPVVGIVGTGDEIVEPGAPLAEDRLYASNIVTLAGWCMRSGL
                     RSLTAIVKDDPDSILAAFRRMSMETDALITSGGAWKGDRDLVADVLERMGWRKIFHQI
                     RMGPGKAVGLGVLNGKPVFILPGGPPSNFMAFLQIAWPGLMRLGGHEEPGLPRVKAKL
                     SAGITEGNAGWTDLFFGTVEETEDLPLFHPMEKRSRLSSIAEATAVASIPEDRDHLAQ
                     GSVISVQLLT"
     gene            1602..2717
                     /locus_tag="PKJ17_06735"
     CDS             1602..2717
                     /locus_tag="PKJ17_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOD75705.1"
                     /translation="MRKSTEHTRQLHRMYALDDERMKGHEGWEKFPLFWFILFLSYRC
                     TRTCEYCYAFNQVGYDNSVEMDDRTFERLLEWIPEVWRVNRTRVNIVVFLGGEPLLRT
                     DRIKNVMDAVYRNTDGMQGMVCTNADLVDSVNWDDLEDIQWISTNITDIPIKELARRM
                     KVIGERSNVINQTIIATLDEFNLERVLDITRFGIESGYRLRYYRNMYWGMDPRYRKAL
                     LERYHELCDMLEDYAIRGYDVHTTFLLDFLIPAWDLESSPYPCGKRMATVFPDGTIGP
                     CIRNHTFKSGTIYDPNPLAKLQVYDFHYDIRRPDLPDECKVCESRTACHAGCPNDKRM
                     FLDSTIGRSVLCDVHREIIPRLRHIESLKRNPSGTER"
BASE COUNT          558 a          791 c          860 g          519 t
ORIGIN      
        1 acgcggtcgt gatgcgcgat gatagaaagg tccttacctg cgcgatggcg gtaagattgt
       61 cgcgggaaca tgatatgtct ttgaaggaga tcggggatgc ctgcaacagg ctgggcatca
      121 agataaggga gtgtcagctg ggctgcttcg cctgatggcc aggcgcccgt ccatccggtg
      181 ccgggtccct gcgctgtctt ggggtggtcc cgggtgacct cccacggcac gaatcgctgc
      241 ggagagccca gggtcttgct ggaggagccc gtgaagagca tgtctatcgg cttcgaagag
      301 gcgttgcgcc tgacgtttga ggcgatagga cctctgcccg ctgagagcgt cggtcttctc
      361 gaatgtgtgg gtcgcgtggc cgcgtcggac ctttttgccc tcgtcgactc cccctcgtcg
      421 gatgtttccc ggaaggatgg ttacgcggtt gtctacggcg aggtgaagga cgccaccgcc
      481 gcggttcccg tccgtctctc cctcgccggt tccacctctg cgggtgcggc aaagaacatt
      541 gtcctcacgg aggggacaac ggtgagggtg ttgaccgggg ccagggtccc ggcgggggcc
      601 gatgccgttg tcgccgacga atacatcagg gccgacggga gggacatcct tatagaatcg
      661 ccgcccgacg aacctgacag gaacatactg gcccggggca gggatgtgcg cgctgggcag
      721 cgcctgatcg aggcgggtcg ccgcatatct ccggccgccg ccggttttct cgccgccgcg
      781 ggtcttggca gggtccctgt cttctcgaag ccggtggtgg ggatcgtcgg gaccggcgat
      841 gagatcgtcg agcccggcgc gccgcttgcc gaggacaggc tctacgcgag caatatcgtc
      901 accctggcag ggtggtgcat gaggtcggga ctgagatcgc tgacggccat cgtgaaggac
      961 gaccccgatt ccatcctcgc cgccttccgg aggatgtcca tggagaccga cgccctcatc
     1021 accagcggcg gggcgtggaa gggcgatcgc gacctggtgg cggatgtcct tgaacggatg
     1081 ggctggagaa agatattcca ccagatccgc atgggaccgg gcaaggccgt gggcctgggc
     1141 gtgctgaacg ggaagcccgt cttcatcctt ccgggagggc cgccgtccaa cttcatggca
     1201 ttcctgcaga tcgcgtggcc gggcctcatg cgcctcggcg gccacgagga acccggtctg
     1261 ccccgcgtga aggcgaagct ctccgcgggg atcacggagg gtaacgcggg atggacggac
     1321 ctcttcttcg ggaccgtcga agagacggag gacctgcccc tgttccatcc catggagaaa
     1381 cgcagccgcc tctcctccat agccgaggcc acggcggtgg catccatacc cgaagaccgg
     1441 gaccacctcg cgcaaggctc ggtgatctcc gtccagctgc tgacatagga cttcgggctg
     1501 cctgatcccc gtccccttcc gccaatcctc tcagaacctc gttgacttcc atggaccttt
     1561 ctcctattaa tattcgaagt catcaaagaa agcgggtaat tgtgagaaaa tcgacggaac
     1621 atacgagaca gctccacagg atgtatgctc tcgatgacga aagaatgaaa gggcacgagg
     1681 gctgggagaa attccccctc ttctggttca tcctcttcct gtcatacagg tgcacgagga
     1741 cctgtgaata ctgctatgcc ttcaaccagg tcggctacga caacagcgtc gaaatggatg
     1801 acaggacgtt cgagaggctc ctggaatgga tccccgaggt ctggagggtc aatcgcacca
     1861 gggtgaacat cgtcgtcttc cttggtggtg aacctctcct gaggacggac aggataaaga
     1921 atgtgatgga cgccgtctac cggaacacgg acggaatgca ggggatggtc tgcaccaatg
     1981 ccgaccttgt ggattccgtg aactgggacg acctcgaaga tatccagtgg atctcgacga
     2041 acatcaccga tatccccatc aaagagctgg ccaggaggat gaaggtgatc ggcgaaaggt
     2101 cgaacgttat caaccagacc atcatcgcca ctctcgatga attcaacctg gaaagggtgc
     2161 ttgacataac acggttcggg atagagagcg ggtatcgtct gaggtactac aggaacatgt
     2221 actgggggat ggacccccgg tacaggaaag cgcttctcga gagatatcac gaactatgcg
     2281 atatgctgga ggattacgcc atcaggggct atgacgtcca caccacgttc ctcctcgatt
     2341 tcctcatccc cgcctgggac ctggaatcct ccccctatcc ctgcgggaag aggatggcga
     2401 ccgtcttccc cgatgggacc atcgggcctt gcataaggaa ccacacgttc aaatcgggga
     2461 ccatctacga tccgaatccg ctggcgaagc tgcaggtcta cgatttccac tacgatatca
     2521 ggcggcccga cctgcccgac gagtgcaagg tctgtgaatc gaggaccgcc tgtcacgcgg
     2581 gatgccccaa cgacaagagg atgttcctgg acagcaccat cgggagatcg gtcctctgcg
     2641 atgtgcacag ggagatcatt ccgcggctaa ggcacatcga gagtctgaaa cgaaaccctt
     2701 cagggacaga gagatagctt acagctcg
//
LOCUS       DAOPOA010000287         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40469_length_2728_cov_2.914703, whole genome shotgun sequence.
ACCESSION   DAOPOA010000287 DAOPOA010000000
VERSION     DAOPOA010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40469_length_2728_cov_2.914703"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PKJ17_06740"
     CDS             <1..615
                     /locus_tag="PKJ17_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75706.1"
                     /translation="RAGILRRDAIEEAVRLGSGGIAYGFLGPRMSKLVTECKLIKQSY
                     GTVEDALSVSPERLAYSLEKLLEVEPEMRSAPISIGIPILLPDGERLIFARRAVKDKS
                     WEERPWAVTTANIDRWAEREWVDLRPANMARWQERFRKASEDACRFPEKRCSRFNRGM
                     LFWKKTEEGKTIIDPGEITAWVFINEFAGGRPHAYSESKEMVYF"
     gene            735..1385
                     /locus_tag="PKJ17_06745"
     CDS             735..1385
                     /locus_tag="PKJ17_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD75707.1"
                     /translation="MTDPRDSAVYLLDLGEPVLIDCGSGHGLERMVENVRKAGFSPAD
                     IKALIMTHCHVDHIGAAPLLRSRFGTRLVIHEKDAEILERGDIRLTAAFCFDVELKPL
                     AVDRKLVGETGTLRFGNHDLTWVHTPGHTPGSISLYMDVDGERILFAQDIGAPLLAEF
                     DCDPAAWLESIQKLFALNADVLCDGHSGAYGPKDVVRQYLEYCLASQYRQGYITAG"
     gene            1777..2214
                     /locus_tag="PKJ17_06750"
     CDS             1777..2214
                     /locus_tag="PKJ17_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75708.1"
                     /translation="MKRAILLTVLVAIATVFLLSLGAGTVKAQAGSGGAQDQPAAPAW
                     RAQMEAADLMISEAEKTKKRCQEEIARAQEMKKRAQEGRARAMAAGGTSTDRPQYPWV
                     TMEKAADKMSADALKTIERCDLQIKQGRALKVEAENELKKLGQ"
     gene            2352..>2728
                     /locus_tag="PKJ17_06755"
     CDS             2352..>2728
                     /locus_tag="PKJ17_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75709.1"
                     /translation="MSIKKLIVLVCAAAALYACFQLFPGGTAALAQTTQLDYSDRRMP
                     LPAFELPIPQDRAEREYLGLSDKGTFKVGDIKSQIVIIEIFSFYCPICQVHAPLVHEV
                     YEKIKARADLKEKIRMIGIAATNT"
BASE COUNT          631 a          761 c          839 g          497 t
ORIGIN      
        1 agggcgggca ttttgcgcag ggacgccatc gaggaggccg tccgtctcgg aagcggcggt
       61 atcgcctacg gcttcctggg gccaaggatg tccaagctcg tcaccgaatg caagcttata
      121 aagcaaagct acggcacagt cgaggacgcc ctttccgtct cgcccgagcg cctggcgtat
      181 tcgctggaga agcttctcga ggtcgagccc gagatgcgca gcgcccccat ttccatcggg
      241 atacccatcc tcctgcccga cggggagcgc ctcatcttcg ccagacgggc cgtgaaggac
      301 aagagctggg aggaacgacc ctgggccgtc accaccgcca acatcgatag atgggccgag
      361 cgggaatggg tggatttgag accggccaat atggcacgat ggcaggagag gttccggaag
      421 gcaagcgagg acgcctgcag gttccccgag aagcgctgct cccgcttcaa ccgcggaatg
      481 ctcttctgga agaagacgga ggaaggcaag acgatcatcg accccggcga gataacggcg
      541 tgggtcttca tcaacgagtt cgcgggcggc aggccgcatg cctacagcga gtcgaaggag
      601 atggtgtatt tttaaatgaa ggttccaggt tccaggttcc aggttccagg tgaaaacgga
      661 aagaaaccgg gtgtgtgttc gtcgtgggac ctcaacggat aaccgacatt atgtggcgcg
      721 ttgccggggc ggatatgacg gatccaaggg acagcgccgt ctatcttctc gatcttggcg
      781 agccggtgct tattgactgc gggtcggggc acggcctcga gcgcatggtc gaaaatgttc
      841 ggaaggcagg tttcagcccc gccgacatca aggctctcat catgacccac tgtcatgtcg
      901 accatatcgg tgccgcgcct ctcctgcgct cccgtttcgg tacaaggctg gtcatccacg
      961 agaaggatgc ggagatcctc gagcgcgggg atatacggct caccgcagcc ttttgcttcg
     1021 atgtcgaact caaacccctt gccgtcgata gaaagcttgt cggggaaacg gggacgctcc
     1081 gcttcggaaa ccatgacctc acgtgggtcc acacgccggg tcacacgccc gggtcgatct
     1141 ccctttacat ggatgtggac ggtgagcgca tcctcttcgc ccaggacata ggggcacccc
     1201 tgctcgccga attcgactgc gaccctgcgg cctggctcga gtccatacag aaactcttcg
     1261 cccttaacgc cgatgtcctc tgcgacggcc attccggagc ctatggcccg aaggacgttg
     1321 tcagacaata cctcgaatac tgcctcgctt cgcaatacag gcagggctat atcaccgccg
     1381 ggtgataccc gcgggcctca agccccgcat atggtcgcat tgtctgcccg gctccttcat
     1441 ggacaacccc gttcaaccgc ccgctgcaca tcccggcgcc tgtcatcgaa aaggatgaca
     1501 gggaaatcat caaaatggct atgcgataat catactttct gcgacagaca aggcggattg
     1561 ccttgattat cctataatca tgagcatcga gcacgttcag atgcagcgtt ggatcgtacc
     1621 tgttaggacc gattgctcca gtgagtatgg caccgaaaag gggaaatgtg ccggggatcg
     1681 aaagaccaga aggaggtgca ggcggtcact accaggttca tcgaaaagga tgaatgacag
     1741 gctacaaaca ttctttacaa acaaggagag ggaactatga aaagagcaat tttgttgaca
     1801 gtgttggtcg cgatagcaac ggttttcctt ctttccctgg gagccggcac ggttaaggcc
     1861 caggcaggca gcggaggggc ccaggaccag ccggccgccc cggcctggag ggcacagatg
     1921 gaagccgcgg atctgatgat atccgaggcg gagaagacca agaagcggtg ccaggaggag
     1981 atcgccaggg cacaggagat gaagaagagg gcccaggaag gacgcgccag ggcgatggcg
     2041 gccggcggga cctcgacaga ccggcctcag tatccatggg tgaccatgga gaaagcggcg
     2101 gacaagatga gcgcggacgc cttgaaaacc atcgaacgtt gcgacctgca gataaaacag
     2161 ggccgggcgc tgaaggtaga agcggagaac gagctaaaga aactgggaca gtaggacaca
     2221 agactttccg agtcccgacg accgcagaga ggtgaacaca gggaggcctc ccgggctcca
     2281 tgagtccgag acggatggtc ccctgtgcct ctttgccttc acgttccatg tcgataacca
     2341 gggaggacgg tatgagcatc aagaagctga tcgttctggt gtgcgcggca gcagcgttgt
     2401 atgcctgttt ccagctcttt ccgggaggaa cggccgcttt ggcccagacc acgcagttgg
     2461 actactcgga cagacggatg cccttgcccg ctttcgaact gcccattccc caggatcgag
     2521 ccgaaaggga atacctgggt ctttcagaca aaggcacgtt caaggtcggc gatatcaaga
     2581 gccagatcgt gatcatcgag atcttcagtt tttactgtcc gatctgccag gtccatgctc
     2641 ccctggtcca cgaggtgtac gagaagataa aggcgcgcgc ggacctgaag gagaagataa
     2701 gaatgatagg catagcggcc acgaacac
//
LOCUS       DAOPOA010000288         2724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40545_length_2724_cov_4.176096, whole genome shotgun sequence.
ACCESSION   DAOPOA010000288 DAOPOA010000000
VERSION     DAOPOA010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40545_length_2724_cov_4.176096"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKJ17_06760"
     CDS             <1..311
                     /locus_tag="PKJ17_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75710.1"
                     /translation="EQYFKMMTHFMLDGSISEDLRERLNDIERSVLDQFDRLFMKMGA
                     KKDVRRFSHAFFAALNGILITYRQHPGKTPEKIRKRMELIARVIARIFENEIRRAADA
                     "
     gene            complement(308..1939)
                     /locus_tag="PKJ17_06765"
     CDS             complement(308..1939)
                     /locus_tag="PKJ17_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOD75711.1"
                     /translation="MSFPTKNNPYSFDEFLAWREGVNYYLDDAFFQKLLRNFCGDEWE
                     SIDKELQRLSHKVSYRWRDFAEKISWPEHRPYIKHYDGHNHRIDRIVRPLETEIMEKE
                     IFSEAFFSERTSPWLRLAKMYLIYQNGESCIACPITCTEGLVALLEKYADTPETKRIL
                     RHCKEGADGDFAIGAQYLSEIQGGSDVPSNVLEAVQEGGQWRLYGTKFFCSATHADYA
                     VVTAKPAGSEDVALFVVPSWLEGNKEKEIRNGYTIDRIKWKMGTSELTTAELTFNGAV
                     AYPVGPLNRGVANVVGIVLTYSRLTVGLSAAAFMARAVREARAYARFREAFGLRIDQF
                     PMLEGQLKNLELFSKRTVSAAFRLYRDFLTLPGGLKGGLVTDETEETKRKRFDVRELI
                     MLQKITSSWDCTDVIRQAMSIFGGHGVMEDFSSLPRLFRDSAINELWEGPRNVLLTQI
                     HRDFQRVSSWYRPSEFVRHILTGADEGLVRRLGAEMEELIACPSLFVMDEKTMEICRR
                     WDAFCHELFHSYQDAALKEVEQAEDTREQGTGTGS"
     gene            complement(1952..>2724)
                     /locus_tag="PKJ17_06770"
     CDS             complement(1952..>2724)
                     /locus_tag="PKJ17_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD75712.1"
                     /translation="FMMTVPMMLQFMLLAPNIDKKDLSAIKYFVCGGSAVPESLIKTY
                     GEKLGIRITHVYGITEYSGAVTFWTADMGADKSNSMGKPVFHGDVKICEPGKNRELPA
                     GEVGEICCAGPQVFAGYWNNPNATAEVLKNGLYRSGDMGKKDEAGFLYVVDRLKDMII
                     SGGENVYPAELETVISAVPGVAEVAVVGKPDKKWGEIPVAFVVKKPGAEVMEQEVLES
                     CKGNLASYKCVREVRFIDAIPKNAVGKVLKKTLKESFT"
BASE COUNT          540 a          835 c          752 g          597 t
ORIGIN      
        1 acgagcagta cttcaagatg atgacccatt tcatgctcga cggatccatc agcgaagatc
       61 tgcgggagag gctcaatgac atcgagcgct ccgtcctcga ccagttcgac cgtctcttca
      121 tgaaaatggg tgcgaaaaag gatgtgcgca ggttttcgca cgccttcttc gcggcgttga
      181 acggcatcct catcacatac aggcaacacc cgggcaagac cccggagaag attcgcaagc
      241 gcatggagct catcgcccgt gtcattgccc ggattttcga aaacgagata cgccgggcgg
      301 cggacgctta agagcctgtg cccgttccct gctccctcgt atcttcagcc tgctccacct
      361 ctttgagcgc ggcgtcctga taggagtgga aaagctcgtg gcagaatgca tcccagcgtc
      421 tgcagatctc catggtcttc tcatccatga cgaacaggct cggacaggcg atgagctctt
      481 ccatttccgc cccgagcctc cggaccagcc cctcgtcggc tccggtcagg atgtgtctga
      541 cgaactcgga cggcctgtac caggacgaga cgcgctggaa atcgcggtgt atctgggtga
      601 gcagtacatt tctcgggcct tcccagagct cgttgatggc agagtctctg aacaggcggg
      661 gaagcgacga gaaatcctcc atgaccccgt ggcctccgaa gatggacatg gcctgtcgga
      721 tcacatccgt gcagtcccag gaggaggtga tcttctgaag catgatgagc tcccgcacat
      781 cgaagcgctt cctcttggtc tcctcggtct cgtcggtcac aagtccgccc ttgagccctc
      841 cgggcagggt cagaaaatca cggtagagcc tgaaggcggc ggacacggtc cgcttcgaga
      901 agagctccag gttcttcagc tggccttcca gcatggggaa ctgatcgatc cggagcccga
      961 aggcctcccg gaatctcgca tacgccctcg cctctctgac cgcccgggcc atgaacgcgg
     1021 cggcggaaag tcccaccgtg agcctggagt atgtgaggac aatgcccact acattggcca
     1081 cgccgcggtt gagtgggccc accggatagg ccacggcccc gttgaaggta agctcggcag
     1141 tggtaagttc cgaggtgccc atcttccact tgattcggtc gatggtatag ccgttccgga
     1201 tttctttctc cttgtttccc tcgagccagg agggcactac gaaaagcgcc acgtcttccg
     1261 agcccgcagg cttggccgtc acgaccgcat agtctgcgtg ggtcgcggaa cagaagaact
     1321 tggtcccgta gagccgccat tgtcctccct cctgaaccgc ctccagcaca ttggagggca
     1381 cgtcggaacc gccctggatc tcggagagat actgggcgcc gatggcgaag tcgccgtcgg
     1441 cgccttcctt gcagtgtctg aggatgcgct ttgtctcggg cgtgtccgca tacttctcca
     1501 gcagggccac cagtccctcg gtgcaggtga ttggacaggc gatgcatgac tcgccgttct
     1561 gatagatcag gtacatcttg gccagcctga gccaggggga tgtgcgttcg gagaaaaagg
     1621 cctcggagaa gatctccttc tccatgatct cggtctccag cggccgaacg atcctgtcga
     1681 tgcggtggtt gtggccgtca tagtgcttga tgtagggccg gtgctcgggc caggagattt
     1741 tttcggcgaa atccctccac cggtaggaca ccttgtgtga cagcctctga agctccttat
     1801 cgatgctctc ccactcatcc ccgcagaaat tccggagcag cttctgaaag aacgcgtcgt
     1861 cgaggtagta gttcacaccc tctctccagg ccaggaattc atcaaaactg tagggattgt
     1921 ttttcgtcgg aaacgacatc gggaaacctc cttatgtaaa gctctctttc agtgttttct
     1981 tcagcacctt gcccactgcg ttcttgggaa tagcgtcgat gaaacggacc tcgcgcacgc
     2041 acttgtaaga ggcgagattc cccttgcacg actccagcac ctcctgttcc atcacctcgg
     2101 cgccgggctt tttcaccaca aaggcaacgg gaatctcccc ccacttcttg tcaggcttcc
     2161 cgaccacggc cacctcggcc actcccggca cggcgctgat caccgtctcc agctcggctg
     2221 gatagacgtt ctctccgccg ctgatgatca tatcttttag gcggtcgacc acatagagaa
     2281 aacccgcttc gtcttttttc cccatatcgc cggaacggta gaggccgttt ttcaggacct
     2341 cggcggtggc gttcgggttg ttccagtagc ctgcaaaaac ctgcggcccg gcgcagcaga
     2401 tctcaccgac ctcgcctgcc ggcagttctc tattctttcc cggctcgcag attttcacat
     2461 cgccgtgaaa gaccggcttg cccatcgaat tggacttgtc tgcacccata tcggctgtcc
     2521 agaacgtgac ggccccggaa tattccgtaa ttccgtacac atgggttata cgaatgccca
     2581 gcttttcgcc gtaggttttg atcagggatt cgggaacggc cgaccccccg cacacgaaat
     2641 atttgatcgc cgaaaggtcc ttcttgtcga tattcggggc aagaagcatg aactgaagca
     2701 tcatgggcac ggtcatcatg aagt
//
LOCUS       DAOPOA010000289         2721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40621_length_2721_cov_3.044636, whole genome shotgun sequence.
ACCESSION   DAOPOA010000289 DAOPOA010000000
VERSION     DAOPOA010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40621_length_2721_cov_3.044636"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKJ17_06775"
     CDS             complement(<1..379)
                     /locus_tag="PKJ17_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD75713.1"
                     /translation="MGPLLSVLDVRKHYEVRRTLFSVAKETIRAVDGVSFHLGVGETL
                     GVVGESGSGKSTLARCVLLLERPDSGEVVFDGVNLLTAGSEEVKRLRKRMQIIFQDPY
                     SSLNPRMKVREIIAEPVRFHGMAK"
     gene            complement(355..1329)
                     /locus_tag="PKJ17_06780"
     CDS             complement(355..1329)
                     /locus_tag="PKJ17_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD75714.1"
                     /translation="MIHTKDNTLLEVTGLNTSFFTGEEVIRAVNDVTFTIGKGEVFGL
                     VGESGSGKTVTAHSIMRLVHPPGRIVSGRIVFEGRDLLELSEKQMEEVRGSRIGMVFQ
                     EPMTALNPVLRIGEQIAETIEIHGVRRGNGVRERTLELLRQVGFDDPEKRYIQYPHQL
                     SGGQRQRVLMAMAVSCDPSLIIADEPTTALDVATESQILGLTCGLVDSLGMSMIFITH
                     DLTIVKSMGRSVGLMYAGRMVERNRVEDFFREPLHPYGRGLLESVYGFSGSAKRLKAI
                     PGSVPKLSELPSGCKFHPRCPFVMDVCKGDEPAMKEVAKDQWVRCYLY"
     gene            1506..>2721
                     /gene="guaB"
                     /locus_tag="PKJ17_06785"
     CDS             1506..>2721
                     /gene="guaB"
                     /locus_tag="PKJ17_06785"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOD75715.1"
                     /translation="MSEKEPTDFREGLTFDDVLLVPASSSIMPADVDVSSYLTPAIRL
                     NIPILSAAMDTVTESKTAICLAQEGGIGIIHRNMTVEDQAHEVEKVKKSESGMIVDPI
                     TIAPENRIQDALELMSRYRISGIPVTKGTRLVGIITNRDLRFETNLERKVQNVMTKEN
                     LVTVKEGIGLEESKKILHKHKIEKLLVVDRNFDLKGLITIKDIEKMRKYPNSCKDQLG
                     RLRVGAAVGVGPDRDERVEALLRAGCNVIVVDTAHGHSKNVIESIRAIKKNFAGVQLI
                     AGNIATSEGCEALIKAGCDAVKIGVGPGSICTTRIVAGIGVPQITAIMDAASVAKRHQ
                     IPLIADGGIKFSGDITKALAAGAQTAMIGNLFAGTDETPGETVLYQGRTYKVYRGMGS
                     LEAMREGKTRDRY"
BASE COUNT          603 a          842 c          769 g          507 t
ORIGIN      
        1 ccttcgccat gccgtggaaa cggacaggct cggcgatgat ctcgcgcacc ttcattcgcg
       61 ggttgaggga tgaatagggg tcctgaaaga tgatctgcat ccgcttgcgc agcctcttga
      121 cctcttcgct ccccgccgtg aggaggttca cgccgtcgaa gacgacctct ccgctgtcgg
      181 gcctttcaag gagaagaaca caccgcgcca gcgtgctctt tcccgagccg ctctcgccca
      241 cgactcccag ggtctcgccg acgccgaggt ggaaactgac gccgtcgacg gcccgtatcg
      301 tctctttggc cacggagaac agggtcctgc ggacctcgta gtgcttcctg acgtctagta
      361 cagatagcag cggacccatt gatcctttgc gacctccttc atggccggct catccccctt
      421 gcacacatcc atcacgaaag gacaccgggg atggaacttg cagcccgacg gcaactcgga
      481 cagcttcggc accgagcccg ggatggcctt gagcctcttg gcgctcccgg agaacccgta
      541 gaccgactcc aacaggcccc ggccataggg gtgcagaggc tccctgaaga aatcctccac
      601 gcggttcctc tcgaccatcc ttcccgcata catgagcccc acggaccggc ccatggactt
      661 cactatcgtc aggtcgtggg tgatgaatat catcgacatc cccagggaat cgacgagacc
      721 acaggtcagg cccagtatct gggactctgt agcaacgtcg agggctgtgg tgggctcgtc
      781 ggcaattatg agcgagggat cgcaggatac ggccatcgcc atgagaacgc gctgtctctg
      841 cccgccggag agctgatggg ggtactggat gtacctcttt tccgggtcgt cgaaccctac
      901 ctgtctcaag agttcaagcg tcctctcccg cacgccgttc ccccggcgga ccccgtgtat
      961 ctcgatcgtc tcggctatct gctcgcctat tcgaaggacg gggttcaggg cggtcatggg
     1021 ttcctggaag accatcccta tgcggctccc tctgacctct tccatctgtt tttcactcag
     1081 ttcgaggagg tccctgcctt cgaagacgat cctgcccgag acgatcctgc ccggcgggtg
     1141 cacaaggcgc attatggagt gggccgtcac ggtcttgccc gaaccgctct ccccgacgag
     1201 accgaagacc tcccccttcc cgatggtgaa ggtcacatcg ttgacggcac gtatgacctc
     1261 ttcgccggta aaaaaggacg tattgagacc ggttacctcg agaagcgtat tatcctttgt
     1321 atgtatcatc gatggcggac ggttcccaac gggaaattta ttttgattat ttgaacctta
     1381 tgcattatac ttaaccatat ttaaatggca agccttttat gtatgagcta acatcttaca
     1441 gcgcatacat ttttttgtat aaagagcacc acacatacac gtatactatc cccatgaggt
     1501 gaatcatgtc agagaaagag ccgacagatt tcagggaagg actaaccttc gacgacgttc
     1561 tgctcgtgcc ggcctcaagc agcataatgc ccgccgatgt tgacgtctca agctacctga
     1621 cccccgccat acggctcaat atccccatac tcagcgccgc catggacacg gtgacggaat
     1681 cgaagaccgc catctgcctc gcccaggagg ggggcatcgg catcatccac cggaacatga
     1741 ccgtggagga ccaggcccat gaggtggaaa aggtcaagaa atccgagagc ggcatgatcg
     1801 tcgaccccat caccatagcc ccggagaaca ggatccagga cgccctggag ctcatgtcgc
     1861 gctaccgcat ctccggcata cccgtcacga agggcacgag gctcgtgggc atcatcacga
     1921 acagggacct gcgattcgag acgaacctgg agaggaaggt ccagaacgtc atgacgaagg
     1981 agaaccttgt caccgtcaag gaagggatcg gcctcgagga atcgaagaag atcctccaca
     2041 agcacaagat agagaaactc ctcgtggtgg acaggaactt tgacctgaaa gggctcatca
     2101 ccataaaaga tatagagaag atgcgcaagt atcccaactc gtgcaaggac cagctgggca
     2161 ggctccgcgt cggcgccgcc gtcggcgtcg gccccgacag ggacgaacgc gtcgaggccc
     2221 tcctgagggc gggatgcaac gtcatagtcg tcgatacggc ccacggccat tcgaagaacg
     2281 tcatcgagtc catcagggcc atcaagaaaa atttcgccgg cgtccagctc attgccggca
     2341 atatcgccac ctcagagggg tgcgaggccc tcataaaggc agggtgcgac gcggtgaaga
     2401 taggcgtcgg ccccggctcc atctgcacca cgcggatcgt agccggcatc ggcgtgcccc
     2461 agataacggc gatcatggac gcggcgtccg tggccaaacg gcaccagata cccctcatag
     2521 cggacggggg catcaagttc tccggcgaca tcacgaaggc cctggcggcc ggcgcacaga
     2581 ccgcgatgat agggaacctc ttcgcgggca ccgacgagac accgggagag acggtcctct
     2641 accagggcag aacgtacaag gtctatcgcg ggatgggctc cctcgaggcc atgcgcgagg
     2701 ggaagacgcg cgaccgctac t
//
LOCUS       DAOPOA010000290         2719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40659_length_2719_cov_3.641517, whole genome shotgun sequence.
ACCESSION   DAOPOA010000290 DAOPOA010000000
VERSION     DAOPOA010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40659_length_2719_cov_3.641517"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1207)
                     /locus_tag="PKJ17_06790"
     CDS             complement(182..1207)
                     /locus_tag="PKJ17_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOD75716.1"
                     /translation="MKRRIYCAHCGTPLGTDFLEGKHRQVCGACDTVYYENPLPVASV
                     ILANGKREILLVRRAKEPFKDMWCCPIGFAETGESIEDAALRELKEEAGVEGRIVQLL
                     DVGSHVNPLYGELLIVTFEVEKTGGQERAGDDASEARYFPVMNLPKLAFDSQVKALAR
                     FVELKKDLWSIHDSFETFVESTVKDRIGYPVVLLSDELANAVQENSEKIVNLWLDDIT
                     TNPSTRSYRRVSREALRSRAMVIMGQFGAWLRKEKSEGEFTSFYRSLGRERNEGGVPL
                     EEAISSLSLLKKHVWMFTYSFGVWEKAVDIYRMFELGERLVYFFDRAAYHTAVGYAGK
                     NTGRPRR"
     gene            complement(1204..1983)
                     /locus_tag="PKJ17_06795"
     CDS             complement(1204..1983)
                     /locus_tag="PKJ17_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD75717.1"
                     /translation="MTERDVQEGSAGIIGNYRNHDLHARVGAIIREHSENRKDIRSVV
                     RDAIPWPRVESVLDLGCGYGWFEEVMPGGLDLIVGIDCLPENEAPFLAAARARAREAV
                     FRAMTLPAPTGLPADRFDLALSLYSLYFFPGAVAEAARVLKPGGLFLCLTHSSSMLEE
                     GQHYFDFRNLRKVIERFSAENGEALLKRHFTTVTCIDYPNALVFREGDEDSLALYIDF
                     KREFIEKDVDPRVVRDTMLSELRRKGELSFNKNDRIFVAGK"
     gene            complement(1994..2608)
                     /locus_tag="PKJ17_06800"
     CDS             complement(1994..2608)
                     /locus_tag="PKJ17_06800"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOD75718.1"
                     /translation="MIVEWNPERPKKKATELIVRTMQEGGIIAYPTDTHYGIGCDLFN
                     IRSIRKLYAMKRLDPKRALSIICRDMKDISTYAVLSDFSFDIVKWYLPGPFTFVLKAR
                     KIIPKLLMTDRKEVGVRIPAHAVPVGIAVLCGRPVINTSARIAGEEVMTDPRVIEKTF
                     GDNISLVVDGGILVSEPSTVVRLADDVAELVREGKGALDDRISR"
BASE COUNT          498 a          871 c          781 g          569 t
ORIGIN      
        1 caggggtatt gcctcaggag cacagaggtg gtaagtatgt ccataccacg tgccagcggg
       61 ccgggggtcc gccctcgaaa aacccacccg gacaccacac cgcccgctat tgcctttgtc
      121 ctataggtta taagaccaat aggactttgg gggctcgggt tttggaatgg gccgggggtt
      181 ttcagcggcg gggtctgccg gtattctttc ccgcgtatcc caccgccgtg tggtatgcgg
      241 cgcggtcgaa gaaatagacg aggcgctctc ccagctcgaa catgcggtag atgtcgacgg
      301 ccttctccca gacgccgaag gaataggtga acatccacac atgtttcttc aggaggctca
      361 aggagctgat ggcctcctcc aggggcaccc caccctcgtt gcgctccctg ccgaggctgc
      421 ggtagaaaga ggtgaactcg ccctcgctct tctcttttct cagccacgct ccgaactggc
      481 ccattatgac catggccctc gaacgcaggg cctccctgct gacgcgccgg taggaacgtg
      541 tcgacgggtt tgtcgtgatg tcgtcgagcc acaggttgac gatcttctcc gagttctcct
      601 gcacggcatt ggcgagctcg tcggacaaaa ggacgacggg gtagccgatc ctgtccttca
      661 cggtgctctc cacgaaggtt tcgaaggaat cgtggatgct ccagaggtcc ttcttcagct
      721 ccacgaacct agccagggcc ttcacctgcg aatcgaaggc aagctttgga aggttcatga
      781 cggggaaata tctcgcctcc gaggcgtcgt cgccggccct ctcctgaccg cctgttttct
      841 cgacctcgaa ggtgacgatg agaagctcgc cgtacagcgg gttcacgtgg gaacccacgt
      901 cgaggagctg cacgatcctg ccctccaccc cggcctcctc cttcagttcg cgaagggcgg
      961 cgtcctctat gctttccccc gtctcagcga aaccgatggg gcagcaccac atgtccttga
     1021 agggctcctt cgcgcggcgc acaaggagta tctcgcgctt gccgttggca aggatcaccg
     1081 acgcgacagg cagcgggttc tcgtaataga ccgtgtcgca tgcgccgcac acctgcctgt
     1141 gcttgccctc gaggaagtcc gtgccgagag gcgttccgca atgggcgcaa tagatccttc
     1201 tcttcatttc cccgccacga atatcctgtc gttcttgttg aagctgagct cgcccttgcg
     1261 gcgaagctcg gagagcatcg tgtccctgac gacgcgggga tcaacgtcct tttcgatgaa
     1321 ctctctcttg aaatctatgt acagggcgag ggaatcctcg tcgccctcgc ggaacacgag
     1381 cgcgttgggg tagtcgatac aggtcaccgt tgtgaaatgc cgcttgagga gcgcctcgcc
     1441 gttctccgcg gagaaccgct caatgacctt tctcaggttc ctgaaatcga aatagtgctg
     1501 gccctcctca agcatggagg agctgtgggt gaggcacagg aagagccccc cgggtttgag
     1561 tacccgcgct gcctcagcga cggcaccggg gaagaaatag agggaataga gggacagcgc
     1621 aaggtcgaag cggtccgccg gcagccccgt cggcgccggc agagtcatgg cgcgaaagac
     1681 cgcctcacgc gcccgggccc tcgcggccgc gaggaacggg gcctcgttct cgggaagaca
     1741 gtctatcccg acgatgagat cgagcccccc gggcatgacc tcctcgaacc agccgtaccc
     1801 gcatccgaga tcgagaacgc tctccacccg gggccagggg atcgcgtcac ggacaacgct
     1861 tctgatatcc ttcctgttct cggaatgctc ccgaatgatg gccccgaccc gcgcgtgaag
     1921 atcgtggttc ctgtagttcc ctatgatccc ggcactccct tcctgcacat ccctctcggt
     1981 cattctgaac cccctaccgg gaaatgcggt cgtcaagggc tcccttccct tccctcacca
     2041 gctccgcgac atcgtcggcg agcctgacca ccgtcgacgg ctcgcttacc aggatccctc
     2101 catccacgac aagcgagatg ttgtccccga aggtcttttc gatcacccgc gggtcggtca
     2161 tgacctcttc accggcgatg cgggcgctcg tattgatgac gggccggccg cacagcaccg
     2221 ctattcccac cggtacggcg tgggccggta tcctcactcc gacctccttc ctgtccgtca
     2281 tgagaagctt ggggatgatc ttcctcgcct tgaggacaaa ggtaaagggc ccgggcaggt
     2341 accatttgac gatgtcgaag gaaaaatcgc tcaagacggc gtaggtgctt atgtccttca
     2401 tgtccctgca tatgatgctg agcgcccttt tcgggtcgag gcgcttcatc gcgtacagtt
     2461 tccgtatgga cctgatgttg aagaggtcgc acccgatccc gtaatgggta tccgtggggt
     2521 aggcgatgat cccgccttcc tgcatggttc gcacgatcag ctcggttgcc ttcttcttcg
     2581 gccgctccgg gttccactca acgatcatct tttcctcgcc accagttgcg ccgtcgctcg
     2641 cattctccct ttatgctccc tcaacccttc atcgtaatgg atgaggtcga acccggggaa
     2701 gagggccgga agttccccc
//
LOCUS       DAOPOA010000291         2709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40837_length_2709_cov_3.515072, whole genome shotgun sequence.
ACCESSION   DAOPOA010000291 DAOPOA010000000
VERSION     DAOPOA010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40837_length_2709_cov_3.515072"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PKJ17_06805"
     CDS             <1..1137
                     /locus_tag="PKJ17_06805"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOD75719.1"
                     /translation="VMWESLNLKDVVALARDPYPSIRQWARDTGKKVVGSTIADVPEE
                     VIYAFDLLPVTILGTHKPLKKAPSHLPDNACSLARSNLELALSYEGDLFSGFVLPQVC
                     DTTQHLSDIWRINFPDKFVVSYLAPRQADRPSARYWVKEEIERLIESLSAWTGKAVND
                     DALRRSIEIHNETRRLLAELYDIKRKNPGTITNKQLFSMMKLSLQVDKAEFNKSLRSI
                     KEGIKPKKGKGYTDVVLVGITCDPPEIFDIFDELKLNIVGDTLVDGTRYLQGAPALNG
                     NLIDALADRHFKRGFFSPIHDNVYKNFEEVKKLYKETKAKAVVYVHIEFCESQEYDLP
                     DLKDMIKKEGIPMHVLDTEYQTTSLSHVLTRAQAFFESLKGGSL"
     gene            1134..2372
                     /locus_tag="PKJ17_06810"
     CDS             1134..2372
                     /locus_tag="PKJ17_06810"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOD75720.1"
                     /translation="MSDKLTSKQLSKKITDEYMEDAFHAHDNNKLVGYTTAISPVEIF
                     VAHDIVPIYPENHAVANLTAKKGVELCSVVEGMGYTSHLCAYARSDLGYRKTGVTVTR
                     GIPDPDLFLACNAQCFTLTKWFQVLARRGDLPVFVFDTPEHILDQKARKEIVKYCVLQ
                     LKELISFLEKVTKRKFDYDRLKEVMKYSAASSILYKKFLDMAQYKPSPISIFDALIGM
                     AIAVYRRGTQECVDYYQTLCDEIQAKVDQGIGVLPKDQEKYRLYWENLPVWFKFSDHA
                     KLLGSYGGVILTSLYVHAWSFDFDLNKDPLETLAENYVNRFSNSTIEDRADMAMELFK
                     KYSMNGMIMFMNRSCKAVSFAVPTLKDVLTKRTGIPALVFESDMGDQRFYAESQVRTR
                     IEAYFETLDRLGLAQKTAAA"
     gene            2520..>2709
                     /locus_tag="PKJ17_06815"
     CDS             2520..>2709
                     /locus_tag="PKJ17_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD75721.1"
                     /translation="MDIGKKIREFRDAKSLNIKQLAELVECTPSLISQLERGKADPSI
                     SMLKRIAEALNVNIVDFFM"
BASE COUNT          682 a          809 c          698 g          520 t
ORIGIN      
        1 gttatgtggg aaagcttaaa tctgaaggat gttgttgcac tcgcgcggga tccgtatcct
       61 tccatccgac aatgggctag agatacagga aagaaagtag ttgggagtac catcgccgat
      121 gttcctgaag aggtgatcta tgcgttcgat ctcctccccg tgactattct tggtactcat
      181 aaacccttaa agaaggcgcc cagtcatctt ccggacaatg cctgttcgct ggctcggagc
      241 aatctcgaac tcgcgctgag ttacgagggc gatcttttca gcggttttgt tctgccccag
      301 gtctgtgaca ccacacagca cctttcggac atatggcgga tcaattttcc cgacaagttc
      361 gtcgtgagct acctggcccc ccgccaggca gacaggccga gcgcccggta ctgggtcaag
      421 gaggagatcg agcgtctcat cgaatccctc agcgcgtgga cgggcaaggc ggtaaatgac
      481 gacgcgctca ggaggtccat cgagatacac aacgagacca ggagactcct tgccgaactt
      541 tacgacatca agaggaagaa cccgggaaca ataacgaaca aacagctctt ttccatgatg
      601 aaactctccc tgcaggtcga caaggccgaa ttcaacaaga gcctgaggtc gatcaaggag
      661 ggtatcaagc cgaagaaggg caaggggtac accgacgtgg tgctcgttgg aataacctgc
      721 gatccgcccg agatattcga catcttcgac gaactcaaac tgaacatcgt cggagacacg
      781 ctcgtcgacg gcacccgcta ccttcagggc gccccggcac tgaacggcaa cctgatcgac
      841 gcgctggcgg acaggcattt caagcggggt ttcttctcac ccatccacga caacgtctac
      901 aagaacttcg aagaggtcaa gaaactctac aaggaaacga aggcgaaggc ggtcgtctac
      961 gtgcacatcg aattctgcga atcccaggaa tatgatctgc ccgacctcaa ggacatgatc
     1021 aagaaggaag gcatcccgat gcacgtcctt gacacggaat accagacgac gtccttgtcc
     1081 cacgtcctga cacgggccca ggcattcttc gaatctctca aagggggatc attatgagcg
     1141 ataagctgac atcgaaacaa ctgtccaaaa agatcaccga cgaatacatg gaagacgcct
     1201 tccatgctca cgataacaac aagctcgtcg ggtacacgac ggcgatctcc cccgtggaga
     1261 tcttcgtcgc ccacgacatc gtgcccatct atcccgagaa ccacgccgtc gccaacctca
     1321 cggccaagaa gggtgtggaa ctctgctccg tcgtcgaagg catgggctac acgagccacc
     1381 tgtgcgccta cgcccggagc gacctgggct accgcaagac gggcgtcacc gtgacaaggg
     1441 gcattccgga ccccgatctt ttcctcgcct gcaacgccca gtgcttcacc ctgaccaagt
     1501 ggttccaggt gcttgcccgc aggggcgacc tccccgtctt cgtcttcgac acaccggaac
     1561 atattctcga ccagaaggcg cgcaaggaga tcgtgaaata ctgcgtcctc cagctcaagg
     1621 aactcatcag tttccttgaa aaggtaacga agaggaaatt cgattacgac cgcctcaaag
     1681 aggtgatgaa atactccgct gcgtccagca tcctttacaa gaagttcctc gacatggccc
     1741 agtacaaacc gtcgcccatc agcatcttcg acgccctcat cggcatggcc atcgccgtct
     1801 accgcagggg tacccaggaa tgcgtcgact actaccagac gctctgcgat gagatccagg
     1861 cgaaagtgga ccagggcatc ggcgtcctgc cgaaggacca ggaaaaatac cgtctctact
     1921 gggagaacct gcccgtctgg ttcaagttca gcgaccacgc gaagctgctc ggctcgtatg
     1981 gcggcgtcat cctcacctcg ctctacgtcc acgcctggag cttcgacttc gacctcaaca
     2041 aggacccgct ggagacactc gcggagaact acgtgaaccg cttctccaac tccaccatcg
     2101 aggacagggc cgatatggcc atggagcttt tcaagaaata ctccatgaac ggcatgatca
     2161 tgttcatgaa caggagctgc aaggccgtct ccttcgcggt ccccaccctg aaggacgtcc
     2221 tgacgaagag aacgggcatc cccgccctcg tcttcgagag cgacatgggc gaccagcgct
     2281 tctacgccga gtcacaggtc cggaccagga tcgaggcata tttcgagacc ctcgacaggc
     2341 tgggtctcgc gcagaagacc gcggcggcat aagcaggtct tcggcacctc cgttaaacaa
     2401 caacgccccg gacagcggct cctgtccggg gtttttcttt gctccccaaa aagaccttga
     2461 cagaattaaa tgtcatttaa taaaataaat attatttaac tcatctcatg gacagcgaca
     2521 tggatatcgg aaaaaagata agggaattca gggacgcgaa gagcctcaac atcaagcagc
     2581 ttgcggaact cgtggaatgc accccctcgc tgatctccca gcttgaacgc ggcaaggcgg
     2641 atccctcgat ctccatgctc aagaggatcg ccgaggccct gaacgtgaac atcgtcgatt
     2701 tcttcatga
//
LOCUS       DAOPOA010000292         2709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40844_length_2709_cov_3.107385, whole genome shotgun sequence.
ACCESSION   DAOPOA010000292 DAOPOA010000000
VERSION     DAOPOA010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40844_length_2709_cov_3.107385"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1988
                     /locus_tag="PKJ17_06820"
     CDS             345..1988
                     /locus_tag="PKJ17_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOD75722.1"
                     /translation="MANNLKISTKLGIGAAVLMVLIFVMGALGYYYLDRSGRSVDHIV
                     NNNYGRIVAFKQVKDAVDTIDKSMLSMALTKDPALKEAEKAKIDQARKSYGEAIKKIE
                     GVMANVKDPKTKAQFDGLLARIKEQLGKGKEHNVKLAKFAMEGKNDEASAIWSEHTST
                     ISNTIDSLFKQLVKLSEERIEIRFNELTKETASARIVFIAISLVIMGLIAAGTFFTVR
                     KITRSLEEGMKVADRLAEGDLTVDVKVTSGDEMGALLQSLGNMVAKWRGVVHEINTSA
                     DNIASASHQLSASAVQMSKGSDDQAQMSMQVATASEEMSQTVIDVARNTGNIAVSGQE
                     TVEVARSGKEVVDMAVREVEEIASTVDASATMVRSLGELSKQIGEIVNVINEIADQTN
                     LLALNAAIEAARAGEHGRGFAVVADEVKKLAERTGNSTAEIADMIGNIQKEVNRAVDA
                     MEDVKKKVSSGADLSQQAGHALNNIVKKVDDLQLMVQQIASATEEMTATSEGISKDID
                     SIANISKETSSASSQVSQASDELSRLSVSLQGVVKGFSV"
     gene            complement(2035..>2709)
                     /locus_tag="PKJ17_06825"
     CDS             complement(2035..>2709)
                     /locus_tag="PKJ17_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD75723.1"
                     /translation="GGMITQPEGFLRKVWTIAKENGLHFITDEVAVGFGRTGSMFACE
                     QEGIEPDFLCLAKGVTGGYLPLAATLTTQEIFGGFLGRFDQFRTFFHGHTYTGNPVAC
                     SVAVENLALFEKERTIERMADKITLLRDELKRFSDLPAVGEVRQRGFMVGIELVKSRK
                     TKRPYPPGAKIGQNVTLAARKRGAIIRPLSDVIVLMPPPGMDAGTLKELVDVTYDSIK
                     EATGAP"
BASE COUNT          613 a          785 c          832 g          479 t
ORIGIN      
        1 tggacctgct atttaattac tattttcaaa gaacaacgtt caaccttgaa gcaagcataa
       61 ttatacttat tttctccggg atgtcaagcg atagtctcaa acttttttta ttttatattt
      121 cccgggaggt gacccgtgga gagtctgccg atatcataac ggattcggta ccctgaacga
      181 agcccccgcc ttccggccgg gcgccgtcgg ggtgccccga ttaataatat ccttttgtct
      241 gccgataaac agtatgcgcg cggaaggcgc caatggaagg aagatcgggc ggttgggacc
      301 gccctcaacc acaaactcat acgggagcgg aggaaggaga catcatggca aacaatctca
      361 agatcagcac gaagcttggt atcggagcgg cagttctcat ggtccttata ttcgtcatgg
      421 gcgccctggg gtattactac ctggaccgct cgggccgttc ggtggaccac atcgtcaaca
      481 acaactacgg acggatcgtg gccttcaaac aggtcaagga cgccgttgac accatcgaca
      541 agtccatgct cagcatggcc ctcacgaagg accctgccct caaggaggcg gaaaaggcaa
      601 agatagacca ggcgcgcaag tcctacggcg aagcgatcaa gaagatcgag ggcgtcatgg
      661 cgaacgtcaa ggacccgaag acgaaggctc agttcgacgg cctcctcgcg aggatcaagg
      721 aacagctggg caagggcaag gaacacaacg tgaagctggc gaagttcgcc atggaaggga
      781 agaacgacga agcatcggcc atatggtcgg agcatacgag caccatatcg aacaccatcg
      841 acagcctctt caaacagctc gtcaagctga gcgaggagcg gatcgagata cggttcaacg
      901 agctcacgaa ggagaccgcc agcgcgagga tcgtcttcat cgccatttcc ctcgtcatca
      961 tgggccttat cgccgcggga acgttcttta ccgtccggaa gatcacgagg tccctcgagg
     1021 agggcatgaa ggtggccgac aggctcgccg agggtgatct cacggtggac gtgaaggtga
     1081 cgagcggcga cgagatgggc gccctcctcc aatcgctggg caacatggtc gccaagtggc
     1141 gtggcgtcgt tcacgagatc aacacgtccg cggacaacat agcctccgcc agtcatcagc
     1201 tgagcgccag cgccgtccag atgtcgaagg gctccgacga tcaggcccag atgtccatgc
     1261 aggtggcgac ggcctcggaa gagatgagcc agaccgtcat cgacgtggcc cgcaatacgg
     1321 gcaacatagc ggtgtcgggc caggagaccg tcgaggtcgc ccgcagcggc aaggaggtcg
     1381 tcgacatggc cgtcagggag gtcgaggaga tagcgagcac cgtcgacgcg tcggcaacga
     1441 tggtccgctc cctgggagag ctttccaagc agatcggtga gatcgtcaac gttatcaatg
     1501 agatagccga ccagaccaac ctattggcgc tgaacgccgc catcgaggcg gcccgcgccg
     1561 gcgagcatgg ccgcgggttt gccgtcgtcg ccgacgaggt caagaagctg gcggagagga
     1621 ccggcaattc gacggcggag atcgccgaca tgatcggcaa catccagaaa gaggtcaacc
     1681 gggcggttga cgccatggaa gacgtgaaga agaaggtgtc ctcgggcgcc gacctttccc
     1741 agcaggcggg tcacgccctc aacaacatcg tgaaaaaggt cgacgacctg cagctcatgg
     1801 tacagcagat agcctccgcc acggaagaga tgacggcgac atcggaaggc atcagcaagg
     1861 acatcgactc catcgccaac atatcgaagg agacatcctc cgcgtcgagc caggtcagcc
     1921 aggcgtccga cgagctctcc cgcctgtcgg ttagcctcca gggcgtcgtg aagggatttt
     1981 ccgtctgaga cagacgaaca ggatcaacaa gacaaggccg gccggcgtgt cctttcaggg
     2041 cgcgccggtc gcttctttta tggaatcgta ggtcacgtcc acgagctctt tgagcgttcc
     2101 ggcatccatg cccgggggcg gcatgagaac gatgacgtcg ctgagcggcc tgatgatggc
     2161 gcccctcttt ctcgccgcca gcgtgacatt ctggccgatc ttcgcgccgg ggggataggg
     2221 ccttttggtc ttcctgctct tgacgagctc gatgccgacc atgaagcccc tctgccggac
     2281 ttcccccacg gcgggcaggt cggagaagcg tttcagctcg tcacgaagga gggttatctt
     2341 gtccgccatg cgctctatcg tccgctcctt ctcgaagagg gcgaggttct caacggcgac
     2401 ggagcaggcc acggggtttc ccgtataggt gtggccgtgg aagaatgtcc tgaactggtc
     2461 gaagcggccg agaaaaccgc cgaagatctc ctgcgtcgtg agcgtggccg cgaggggaag
     2521 gtaccccccg gtgaccccct tggcaaggca gaggaaatcg ggttcgatgc cttcctgttc
     2581 gcaggcgaac atggacccgg tgcgcccgaa acccacggcc acctcgtcgg tgatgaaatg
     2641 gagcccgttc tccttcgcga tggtccagac cttcctcagg aaaccctcgg gctgggtgat
     2701 catcccacc
//
LOCUS       DAOPOA010000293         2705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_40919_length_2705_cov_3.421509, whole genome shotgun sequence.
ACCESSION   DAOPOA010000293 DAOPOA010000000
VERSION     DAOPOA010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40919_length_2705_cov_3.421509"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="PKJ17_06830"
     CDS             complement(<1..1256)
                     /locus_tag="PKJ17_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOD75724.1"
                     /translation="MDSPELNTRIIERGKELYELLEGEKPSLFQRSRWVGRIMDWLLR
                     HEELRTGVFRFVDVFPSLATSSMLVDHLHDLGSSDSLQSSLRWVLRHASRGGWLTRFA
                     LAKTVRYTMRKLGEQFMVGENAREAVRTLARIRQEGCTFSVDILGESVLSGAEAEAYR
                     RLYIDLVEVLSRAGSEWPPLGVPEKTNDLDWGSHPKFSISLKPTSLYSQARPVDFDNS
                     VEAVVMRIRPVYEKVLESGGSLCIDMESYQHKDLSLEVFKRLRAGYPDRSHLAVAIQA
                     YLPDTDNDLRQLLDWSEKQGLPVSIRLVKGAYWDHEVMRARQNGWDIPVYMNKSETDA
                     AFERNALRVLSHPTSYLACASHNIRSIAAVMETARSLGVPDSRYEFQMLYGMAEPIQR
                     VLARITGRVRLYCPCGQIVPGMAYLVR"
     gene            complement(1271..1981)
                     /locus_tag="PKJ17_06835"
     CDS             complement(1271..1981)
                     /locus_tag="PKJ17_06835"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOD75725.1"
                     /translation="MAIRLKVFRFDPEKDEEPYYKVYAVNAGPTDRLLDCLNRIRWEQ
                     DSTLAFRMSCGHGVCGSDGMRIDGICGLPCQRLVGSLGPEALIEPLPVFRVIKDLIVD
                     LTPFFERYRSIKPYLISASPPPDDERRQSPDEVRLLESAVRCILCACCTASCPVNQNP
                     ETADYVGPAALVRAFRYLFDSRDQGFVERVPVLDRKQGAWGCQTLWKCTEVCPKQIPV
                     TKSIGQIKRRIHEAKKGT"
     gene            complement(1998..>2705)
                     /locus_tag="PKJ17_06840"
     CDS             complement(1998..>2705)
                     /locus_tag="PKJ17_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOD75726.1"
                     /translation="IPVAPTCHYLMGGIPTDTDGRVLADEKGRVVPGLFAAGECACVS
                     VHGANRLGCNSLLDLLVFGRRAGRVMKEDVQGIEVPASRPDPVEAVKERLAALMGHDG
                     VERSHLIRQDMQAIMTDHGSVFRTEESLKQGIDAILALKERFSRVAVTNKGKSFNYDL
                     METIELGHQLDLCEVILVSARNRKESRGAHFREDFPERDDRNYLRHTLIFDAPGGPRI
                     EYKPVAITRFQPQVRAY"
BASE COUNT          577 a          801 c          764 g          563 t
ORIGIN      
        1 cgcacgagat aggccatccc gggcacgatc tgtccgcagg ggcagtacag ccgcacccgg
       61 ccggttatcc gcgcgaggac ccgctggata ggttcagcca tcccgtagag catctgaaac
      121 tcatagcgac tgtccggtac gcccaaagat ctggccgtct ccatcacggc cgcgatggaa
      181 cgtatattgt gggatgcgca ggcaagatag gaggtagggt gggaaagtac acgtagcgca
      241 ttgcgctcga aagcggcgtc agtttcggac ttgttcatgt acaccgggat gtcccaaccg
      301 ttctgcctgg ccctcatcac ctcgtggtcc caataggcac ccttcacgag gcggatggag
      361 acgggaagcc cctgtttttc actccagtcg agaagttgcc gaagatcgtt gtccgtgtcg
      421 ggcaaatagg cctgtatggc gacagcgagg tgggagcggt caggataccc ggcgcgcagg
      481 cgcttgaaga cctcgagcga caggtctttg tgctggtacg attccatgtc gatacagaga
      541 gagccgccgc tttcgagcac cttctcgtag acaggccgga tgcgcatcac cacggcctcg
      601 acggaattat cgaaatccac gggcctcgcc tgggaataca gggacgtggg cttaagggag
      661 atgctgaact ttggatgtga tccccagtcg agatcatttg ttttttcagg aacgcccagc
      721 ggcggccatt cgctcccggc ccgcgacaac acttccacaa gatcgatata gaggcggcgg
      781 tacgcttccg cttcagcccc gctgaggacc gattccccca ggatatcgac ggaaaaggtg
      841 cagccttcct gccgtattcg ggccagcgtt cgaacggcct cacgtgcatt ctcgcctacc
      901 atgaattgtt cgcccagctt tcgcatcgtg taccgaactg tcttagcgag cgcgaacctg
      961 gtcagccaac ctccccggga ggcgtgacgc aacacccatc ggagcgaaga ttgcaaacta
     1021 tcggatgaac cgagatcatg gagatggtcg acaagcatcg aggatgtggc aagggaagga
     1081 aagacatcga cgaagcgaaa gacgccggtg cgaagctctt cgtgcctgag cagccaatcc
     1141 attatcctgc caacccagcg cgatctttga aaaagggatg gtttctcgcc ctcgagcaac
     1201 tcatagagct ctttccctct ttcaatgatc cgtgtgttca gttcaggact gtccatcggc
     1261 acctgttcat tcaagttccc ttctttgcct catggatcct ccgcttgatc tggccgatcg
     1321 acttcgtaac agggatctgc ttggggcaga cttccgtaca tttccacaag gtctggcaac
     1381 cccaggcgcc ctgtttccgg tcgaggaccg ggacacgctc gacaaaaccc tggtcccttg
     1441 agtcgaagag atagcgaaag gcccggacaa gagcggcagg ccccacgtaa tccgcggttt
     1501 ccgggttctg gtttaccggg cacgatgcgg tgcagcaggc gcagaggatg cagcgcactg
     1561 cactttccag caggcgaacc tcatccgggg actgccggcg ttcatcatca ggcggtggcg
     1621 acgcggagat aaggtatggt ttaatggaac ggtatctttc aaagaagggc gtgaggtcga
     1681 cgatgaggtc cttgatcacc ctgaatacgg gcaggggctc gatgagggcc tccggaccga
     1741 gtgatccgac gagcctttga cagggcaggc cgcatatccc gtcgatccgc atgccatccg
     1801 aaccacagac gccatggccg caggacatac ggaaggcgag agtggagtcc tgctcccacc
     1861 tgatacgatt gagacaatca agaagccggt ctgtgggccc ggcgtttacc gcatagactt
     1921 tatagtatgg ttcttcatcc ttttccggat cgaagcgaaa aaccttcaac cgtatagcca
     1981 cggaccacct cccgcgatca gtatgctctc acctgaggtt gaaacctggt tatcgccacc
     2041 ggtttatact ctatccgcgg cccacctggt gcgtcgaata taagagtgtg cctcagatag
     2101 ttccggtcat ctctttcagg gaaatcctcc cgaaagtggg cgccccggct ttcttttcgg
     2161 ttacgtgcgc tcacaaggat cacttcgcaa aggtcaagct gatggccgag ttcgatcgtt
     2221 tccatgagat cgtaattgaa cgatttcccc ttattcgtga ccgccacacg ggaaaacctc
     2281 tccttgagtg ccaaaatagc atcgatcccc tgtttcaggc tttcttccgt gcgaaagacg
     2341 gaaccatggt ctgtcataat ggcctgcatg tcctgtcgta tcagatggga ccgctccacc
     2401 ccgtcatggc ccatcaacgc ggcgagtctc tctttgaccg cttccaccgg gtcgggacgc
     2461 gatgcgggca cctcgatccc ctgtacgtcc tccttcatca ccctcccggc gcgccttccg
     2521 aagacgagaa ggtcgagaag tgagttgcac ccgagccggt ttgcaccgtg gacggataca
     2581 catgcacatt cacccgcagc aaagagcccg ggcacgactc gccccttctc gtcggcgagg
     2641 accctgccgt ctgtatccgt ggggatccca cccatcaggt agtggcaggt gggtgccacg
     2701 ggaat
//
LOCUS       DAOPOA010000294         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41134_length_2695_cov_3.112121, whole genome shotgun sequence.
ACCESSION   DAOPOA010000294 DAOPOA010000000
VERSION     DAOPOA010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41134_length_2695_cov_3.112121"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PKJ17_06845"
     CDS             complement(<1..884)
                     /locus_tag="PKJ17_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOD75727.1"
                     /translation="MFADSVMSGLNGLTAGFANLHWSHPVMMIIGGLLLYLGIKKDCE
                     PLLLVPIGFGCILINIPLAGLMDKEGFLRIIYDAGVITELFPLFIFVGIGAMTDFGPV
                     LENPYTFLLGAAGQFGIFITLLLALALGFPYKDAVTIGIIGACDGPTVIYVASQYAQH
                     LLGPVTVAAYSYMALVPVLQPPIMRMLTTQKERTTVMKSHAKSVSKTTKMLFPIVITI
                     VGGLIAPKGLPLLATIMLGNLMKESGVVSRLTSAAENEIANIVTLLLGISIGATMSGP
                     VFVQPKTLIILILGLLAI"
     gene            complement(1277..1624)
                     /locus_tag="PKJ17_06850"
     CDS             complement(1277..1624)
                     /locus_tag="PKJ17_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75728.1"
                     /translation="MNDGKKKGIILIIIGICIPLFALPFVSGLDINKGFMDNFYNAGI
                     RITKSAVPAPPETAAGKPSGDTAGKNPLSLERMRIERIPFRFFLVPTFILLYIGIVMI
                     DRAKSREKKTGHV"
     gene            1752..>2695
                     /gene="ispG"
                     /locus_tag="PKJ17_06855"
                     /gene_synonym="gcpE"
     CDS             1752..>2695
                     /gene="ispG"
                     /locus_tag="PKJ17_06855"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOD75729.1"
                     /translation="MKRKKTRQMHVGGIPIGGGSPIVVQSMLKTNPENFIETLEQTRE
                     LKKAGCELVRIALPQEETCKIIPFLRKEVDVPIIGDIHFNHKIALKAMELGIDGIRVN
                     PGTINNLRKMREIATAALETKTPVRLGMNIGSMEKRVLKKYTRPSADAMVESALYHVA
                     LFEDVGWTELKVSLKASDIRETIDAYRKFSRKSDYPLHVGITEAGPVFSGAIKSAIGI
                     GILLHEGIGDTIRVSLTGNPVYEVIAAYHILRDLGLRKRGINIISCPTCGRTKVDLAA
                     IVEEFEKEINDFDSYLDVAIMGCEVNGPGEAREADIGLA"
BASE COUNT          693 a          698 c          763 g          541 t
ORIGIN      
        1 catatggcga gaagcccgag gataaggatg atgagggtct tgggctgcac aaagaccggc
       61 ccgctcatgg tggcgccgat ggatatgccg aggaggagtg tcacgatatt ggctatctcg
      121 ttttccgccg cactcgtcaa cctgctgacg acccccgatt ctttcatgag gttgccgagc
      181 atgatcgtcg ccagaagagg gaggcccttg ggggcgataa gaccgccgac gatggtgatg
      241 acgatgggaa agagcatctt cgtcgtcttg gacacgcttt ttgcgtggct cttcatgacc
      301 gtggtacgtt ccttctgcgt cgtcagcatc ctcatgatgg gaggctggag gaccgggacg
      361 agggccatgt aggaataggc cgccactgtc acgggcccta acaggtgttg agcgtactgc
      421 gaggccacgt aaatgacggt gggaccgtca caggctccta tgatgccgat tgtcacggca
      481 tccttgtaag gaaagccgag agcaagggcg agaaggaggg tgatgaatat gccgaactga
      541 cctgccgcac ccaggaggaa ggtataaggg ttctccagga cgggtccgaa atccgtcatc
      601 gcccctatgc cgacgaatat gaaaagggga aagagttccg tgatgacacc ggcatcgtag
      661 atgatgcgca aaaatccctc tttgtccatc agtccagcga ggggtatgtt gatgaggatg
      721 catccgaacc cgatgggaac cagaagcagg ggctcgcaat ctttcttgat ccccaggtac
      781 agcaacaacc cacctatgat catcatcacg ggatgcgacc agtgcaggtt cgcgaaacca
      841 gccgtaagtc cattcaaacc gctcattacc gaatcagcaa acatgcccca atcctccctt
      901 tatttgtctt cttctttcct gtagggaaag atccttttga gagcagacat aatgagactg
      961 aaaacaatga gggtacatat ggtaccgccc atcccgacaa caaccatggt gaagccaaag
     1021 gtccatttgt ccatagtaac ccgtcctccc ggtttggtgg tttttatgtg aataattaaa
     1081 ggtgccgaaa ttgctgtaaa acactatctc aaccacttac ctatagtcaa gtgaaattgt
     1141 taacgaggag gcgcattgtt gagcaaaagg aaggtgccat attgtccgct ctcggggaat
     1201 ccggaagaac cacgccgaca ccttaaaaaa aagggggggg gaggaaagga caacggaata
     1261 cgagcgggaa cagacactac acgtgcccgg tcttcttctc ccttgatttt gccctatcga
     1321 tcatgactat ccctatgtat aaaaggatga aggtgggaac aagaaagaac ctgaacggta
     1381 tcctttcgat cctcattctc tcaagagaaa gagggttctt ccccgcggtg tctcccgacg
     1441 gcttcccggc ggcggtttca ggcggtgcgg gaacggcact cttcgttatc ctgatgcccg
     1501 cgttgtagaa attatccata aaccctttgt tgatgtccaa accggagaca aagggcaggg
     1561 caaagagggg aatgcagata ccaatgatga tgagaatgat gccttttttc ttgccgtcat
     1621 tcatgattgg ttcactatac cataggtcgg acggcagaag gacacttttt tgcctacgct
     1681 gatgattgac atcgggtccc ggtggggata tgataagcaa gattcagcca cttcaattca
     1741 aggaacaggg gatgaaacgt aaaaagacaa gacagatgca cgtcgggggt atccccatag
     1801 gcgggggcag ccccatcgtc gttcaatcca tgctcaagac caatcccgag aatttcatag
     1861 agaccctgga acagacgcgg gaacttaaga aggccgggtg cgaactcgtc aggatcgccc
     1921 tgccccagga agaaacgtgc aagataatcc ccttcctcag aaaggaagtg gacgtaccca
     1981 tcatcggcga cattcacttc aaccacaaga tagcgctgaa ggccatggaa ctcggcatcg
     2041 acggcataag ggtcaacccc ggcaccatca acaacctgcg caagatgcgg gagatcgcga
     2101 cggcggccct ggaaacgaag acgcccgtga gactcgggat gaacatcggt tccatggaga
     2161 aaagggtcct caagaagtat acgaggccaa gcgcggacgc catggtcgaa agcgccctgt
     2221 accacgtggc cctctttgag gatgtgggat ggacggagct caaggtctct ctcaaggcat
     2281 cggacatcag ggagaccatc gacgcttaca ggaagttctc caggaagtcc gattatcccc
     2341 ttcacgtggg gataacggag gcgggccccg tcttttccgg agccatcaag tcggcgatag
     2401 gcatcggcat actgctccac gagggcatag gagacaccat cagggtctcg ctgaccggca
     2461 atcccgtgta cgaggtgata gccgcctatc acatcctgcg cgacctgggc ctgaggaaac
     2521 gaggcatcaa catcatctcc tgccccacct gcggcaggac gaaagtggac ctcgcggcga
     2581 tagtcgagga gttcgaaaag gagatcaacg acttcgattc gtacctggac gtggccatca
     2641 tgggctgtga ggtcaacggc cccggcgagg cgcgcgaggc ggacataggc ctcgc
//
LOCUS       DAOPOA010000295         2679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41447_length_2679_cov_3.985518, whole genome shotgun sequence.
ACCESSION   DAOPOA010000295 DAOPOA010000000
VERSION     DAOPOA010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41447_length_2679_cov_3.985518"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKJ17_06860"
     CDS             complement(<1..502)
                     /locus_tag="PKJ17_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOD75730.1"
                     /translation="MDKFIIEGGERLKGKVRISGSKNAVLPVLAATLLQRGTYAISNV
                     PRLMDVTTMMQLLDLLGARCVWQDEHTVEVDTAEVGNHVAPYELVKKMRASVLVLGAL
                     IGGLRKATVSYPGGCAIGERPIDLHLKGLSALGCDVAIREGYVDVTAKKLKGARIVFD
                     SVTVGGT"
     gene            complement(495..1313)
                     /gene="prmC"
                     /locus_tag="PKJ17_06865"
     CDS             complement(495..1313)
                     /gene="prmC"
                     /locus_tag="PKJ17_06865"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOD75731.1"
                     /translation="MRISQIIAGERGLSRTDVIATVCLALGLTKEQLFCRMDREIGGE
                     DLGAVRKALDERAAGRPLAYMTGTREFFSETFSVNEAVLIPRPETEVLVEEALALIGG
                     RRDLRILDMGTGSGAIGIVIAKHAGSSVVCVDISEAALAVARRNARSIGVEERTRFVC
                     GDLFAAFSDGTRFDMVLSNPPYVADDEWDELMADVRDFEPARALRGGEGGLEVYRRIA
                     AELPRRLAPGGSVLLEIGSEAQAGTVGAMLEVAGLRVKVRKDYSGRERVLAGHG"
     gene            complement(1320..2384)
                     /gene="prfA"
                     /locus_tag="PKJ17_06870"
     CDS             complement(1320..2384)
                     /gene="prfA"
                     /locus_tag="PKJ17_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOD75732.1"
                     /translation="MFGKLIELEKRFDEIEAEMVKPEVLSNMEEYKKLAKERTDLKEV
                     VDPYREWKAAKAEHEKTSELLKQESDEDMKTLAREELASLQKEMERIEGILTDSLLRK
                     TEKQAKSMFLEIRAGTGGEEAALFARDLFASYMKFAERMRWKTEIMSSSPSDLGGFKE
                     VIVVIEGKDAFNTLRYESGVHRVQRVPETEAQGRIHTSTITVAVLPEPEELEVTINPD
                     EVRVDLFRSSGPGGQHVNTTDSAVRLTHIPTGMVVTCQDEKSQHKNKAKAMRVLRARL
                     KEKMEGEKEQEISDERRKQVGTGERSERIRTYNFPQGRVTDHRIGLTLYKLQDILNGN
                     IEEITGPVTAHFRSEALKKG"
     gene            complement(2384..2590)
                     /gene="rpmE"
                     /locus_tag="PKJ17_06875"
     CDS             complement(2384..2590)
                     /gene="rpmE"
                     /locus_tag="PKJ17_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOD75733.1"
                     /translation="MKKGIHPELRTAMVKCACGHTFETLSVKDKITVEICAKCHPIFT
                     GKEKRLDSAGQVEKFERKYGKKQK"
BASE COUNT          431 a          894 c          745 g          609 t
ORIGIN      
        1 ccgtcccgcc gacggtgacg ctgtcgaaga cgatgcgcgc ccctttaagc ttctttgccg
       61 tgacatcgac ataaccctcc cggatggcga cgtcgcagcc cagcgccgag aggcccttga
      121 ggtggaggtc gatgggccgc tcccctatgg cgcaccctcc ggggtaggag accgtcgcct
      181 tcctgaggcc gccgatgagc gccccgagaa cgagaaccga ggcccgcatc ttcttcacca
      241 gctcgtaggg agcaacgtgg ttccccacct ccgcggtgtc tacctctacc gtgtgctcat
      301 cctgccatac acacctcgca ccgagcaggt cgagaagctg catcatcgtc gtgacatcca
      361 tgagacgggg cacgttactg atggcgtacg tgcccctctg aagcagcgtg gcggcaagaa
      421 cgggcaggac ggcgttcttg gagccgctta tcctgacttt ccccttaagc ctctctccgc
      481 cttctataat gaacttatcc atgtcccgcg agcacccttt ccctgcctga atagtctttt
      541 ctcactttca ccctgagccc cgccacctcg agcatggcgc cgaccgtccc ggcctgcgct
      601 tcggagccta tctccaggag gaccgatccg cccggggcaa gacgccgggg cagctcggcg
      661 gcgatccgcc ggtacacctc gagaccccct tccccgcccc gcagggcacg ggctggctcg
      721 aaatccctca catcggccat aagctcgtcc cactcgtcgt cggccacata gggggggttc
      781 gacaggacca tgtcgaagcg tgtcccgtcg ctgaatgccg cgaaaaggtc gccgcacaca
      841 aagcgcgtcc tttcttcgac cccgatggag cgggcattcc tgcgcgccac cgcgagggcc
      901 gcctcggata tgtcgacaca gacgacgctg ctgcccgcgt gtttcgcgat aacgatgccg
      961 atcgccccgg aacccgtccc catgtcgaga atcctgaggt ccctcctgcc gccgatgagg
     1021 gcgagggcct cctccacgag gacctccgtc tccggccgcg ggatcagcac cgcctcattc
     1081 acggagaagg tctcggaaaa gaactcccgc gttcccgtca tatacgccag gggcctgccc
     1141 gcagcgcgct cgtcgagggc cttccgcacg gcgccgaggt cttccccgcc gatctcacgg
     1201 tccatgcggc agaagagctg ttccttcgta aggccgaggg cgaggcatac ggttgcgatg
     1261 acgtccgtcc tggagagacc cctctcgcct gcgatgatct ggctgatcct caatgccctt
     1321 tacccttttt tgagtgcttc ggagcggaaa tgcgcggtca cgggacccgt tatctcctct
     1381 atgttcccgt tgaggatgtc ctggagtttg tacagggtga ggccgatcct gtggtcggtg
     1441 acgcgtccct gggggaagtt gtaggtcctg atgcgctcgc tgcgctcgcc ggttcctacc
     1501 tgttttcgcc gctcgtcgga gatctcctgc tccttctcgc cttccatctt ttccttgagc
     1561 cgggccctga ggacgcgcat ggcctttgcc ttgttcttgt gctgcgattt ctcatcctgg
     1621 cacgtgacga ccatgcctgt cgggatgtgg gtaagcctca ccgccgagtc ggtggtgttc
     1681 acgtgctgcc ccccgggccc gctggaacgg aagaggtcga cgcgcacctc gtcgggattg
     1741 atggtgacct ccagctcctc cggctcgggg aggacggcaa cggtgatcgt cgaggtgtgg
     1801 atccttccct gggcctccgt ctcggggacc ctctgcacgc gatggacgcc gctctcgtaa
     1861 cgcagggtat tgaaggcgtc ctttccctcg atgacgacga tcacctcctt gaaacctcca
     1921 aggtcggagg ggctggaact catgatctcc gtcttccatc tcatgcgctc ggcgaatttc
     1981 atgtatgagg cgaaaaggtc gcgcgcgaag agggcggcct cctcccctcc cgtgccggcc
     2041 cgtatctcga ggaacatgct cttcgcctgc ttctcggtct tcctcaggag gctgtccgtc
     2101 aggatccctt cgatcctttc catttccttc tggagactgg caagctcctc ccgcgccagg
     2161 gtcttcatgt cctcgtcgga ctcctgcttc agaagctccg acgtcttctc atgctcagcc
     2221 ttcgcggcct tccattcgcg gtagggatcg acgacctcct tgaggtccgt gcgctccttg
     2281 gcaagcttct tgtactcctc catgttggag agcacctcgg gcttcaccat ctccgcttct
     2341 atttcatcga agcgtttctc aagctcaatg agtttgccga acattacttc tgtttctttc
     2401 cgtatttcct ttcgaacttc tcgacctgac cggcggaatc aaggcgtttt tccttgcccg
     2461 tgaagatggg gtggcacttg gcgcatatct cgacggtgat cttgtccttc actgaaaggg
     2521 tctcaaacgt gtgaccgcag gcgcacttca ccatcgcggt cctgagttcc gggtggatgc
     2581 cctttttcat gtctcttatc ctcctttgct catcgaatag agaaagtctt tattagaatc
     2641 cgtatcggcc aatttttcaa ggagaaattc catggcctc
//
LOCUS       DAOPOA010000296         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41494_length_2677_cov_2.945843, whole genome shotgun sequence.
ACCESSION   DAOPOA010000296 DAOPOA010000000
VERSION     DAOPOA010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41494_length_2677_cov_2.945843"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..148)
                     /locus_tag="PKJ17_06880"
     tRNA            complement(75..148)
                     /locus_tag="PKJ17_06880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Gln,seq:ctg)
     gene            376..2067
                     /locus_tag="PKJ17_06885"
     CDS             376..2067
                     /locus_tag="PKJ17_06885"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOD75734.1"
                     /translation="MTEQDVSPQSDFIREIIDNDLKAGKHDAVATRFPPEPNGYLHIG
                     HAKSVCLNFGIAAQYKGTCNLRMDDTDPAGESLEFVESIQNDIRWLGFDWEGRLFFAS
                     DYFEALYRFAVTLIKRGKAYVCDLRADEIRAYRGTLTEPGKNSPYRDRSIEENLDLFA
                     RMRAGEFEDGSRVLRAKIDMASPNVTMRDPVIYRIKRSGHYRIGDEWTIYPMYDFAHC
                     LSDSIEKITHSICTLEFENNRPLYDWFVNELVEGPKPRQIEFARLNLTYTVLSKRRLI
                     ELVESRMVSGWDDPRMPTVAGMRRRGYTPEAIREFCARIGVAKNDNLVDIALLEHCVR
                     EDLNERAPRAMAVLKPLKVIIDNYPEGQAEDMECANHPQRPEMGTRKVPFSRVLYIER
                     DDFMENPPKKYKRLGPGREVRLRNSYVIRFASMVKDEETGAVSELHCTYDPGTRNAPP
                     SDGRKVEGVIHWVSAAHAIPATVRLYDRLFRVPDPAGTEGDFASLLNPGSLEVVPAYV
                     EPGLAGIVQESRYQFERIGYFCVDTKDSLPEKPVFNRIVSLRDSWAKIAAEPAGK"
     gene            2120..>2677
                     /locus_tag="PKJ17_06890"
     CDS             2120..>2677
                     /locus_tag="PKJ17_06890"
                     /inference="COORDINATES: protein motif:HMM:NF020477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HOD75735.1"
                     /translation="MNFKQGFDNEKYLQEQTREILERVERFDNKLYLEFGGKLLYDYH
                     AARVLPGYDPNVKMRLLAELKDKADLLLCIYAGDIERKKIRADFGITYDADALKLIDE
                     LRHWGINVLGVIITRFDNQPAAVHFRNKLERRGIRVYTHRFTRGYPTDVDTIVSDEGY
                     GANEYMESDKPLVIVTGPGPGSGKLA"
BASE COUNT          656 a          761 c          745 g          515 t
ORIGIN      
        1 cgtcttcaga aatcactttg tcaacagcct gtcaagcgat tcaagccatt caagcgattg
       61 caacggtctt ttgttggctg ggggacaagg attcgaacct cgatagcaag gtccagagcc
      121 ttgcgtcctg ccgttagacg atcccccaga gggtttgcga agtggcgacc gcctttaagg
      181 tctgtcctat acaaagcata tcacgaggcc tttgttaacg ggtgagggct cgtcggccgg
      241 agactcgcct acgactataa cacattgaaa aagatggttg tttgtggtaa gaaatggtcg
      301 gtcgtcgttc atgcaggaca cgcggacggg gccgaaatcc aatacaggaa gcatgtagct
      361 cgaggaaaac ctactatgac agaacaagat gtttccccgc aaagcgattt cataagagag
      421 atcatcgaca atgacctcaa ggccggcaaa catgacgcgg tggccacgcg ttttccgccg
      481 gaaccgaacg gttatctcca catagggcac gcgaaatccg tttgtctcaa tttcggtatc
      541 gccgcccagt acaagggaac gtgcaacctg cgcatggacg atacggaccc cgctggcgaa
      601 tccctggagt tcgtggaatc catccagaac gacatccgct ggctcggctt cgactgggag
      661 gggcggctct tcttcgcctc cgattacttc gaagcgctct accggttcgc cgtgaccctc
      721 atcaaaaggg gaaaggctta cgtctgcgac ctcagagccg acgaaatcag ggcctaccgg
      781 ggtacattaa cggaaccggg gaagaacagc ccctatcgcg acaggtccat cgaggagaac
      841 ctcgacctct tcgccaggat gagggcgggg gagtttgagg acggcagccg cgtgctgcgt
      901 gcgaagatcg acatggcatc gcccaacgtc accatgcgtg accccgtcat ctaccggatc
      961 aagcgctccg gccactaccg cataggcgac gagtggacca tctatcccat gtacgatttc
     1021 gcccactgtc tctccgattc catcgagaag atcacccatt ccatatgcac cctggagttc
     1081 gagaacaaca gacccctgta cgactggttc gtgaacgagc tcgtagaagg tccgaaaccc
     1141 cgccagatag agtttgctcg cctcaatctc acgtataccg tcctcagcaa gcgcaggctc
     1201 atcgaactcg ttgaaagccg catggtcagc ggctgggatg accctcgcat gcctaccgtc
     1261 gccgggatga gacgacgggg atacaccccg gaggcgatac gggagttctg tgcgagaata
     1321 ggcgtcgcca agaacgacaa ccttgtcgat atagctctcc tggaacactg cgtccgtgag
     1381 gatctcaacg agcgcgcacc gcgcgccatg gccgtcctca aacccctcaa ggtgatcatc
     1441 gacaattatc ccgaaggaca ggcggaggac atggaatgtg ccaatcatcc tcaaagacct
     1501 gaaatgggca caagaaaggt gcctttttcc cgggtcctct acatcgaaag ggatgatttc
     1561 atggagaacc ctccgaagaa gtacaaacga ctcggtcccg gacgcgaggt gcgtctgagg
     1621 aactcctacg tcatcaggtt cgcctccatg gtcaaggacg aggagacggg agcggtcagc
     1681 gagcttcatt gcacttacga tcccgggacg aggaacgcac ccccgtccga tggacgcaag
     1741 gtcgagggtg tcatccactg ggtttccgcg gcgcacgcca tacccgcaac ggtccgtctc
     1801 tacgacaggc ttttcagggt ccccgacccc gccggcaccg aaggtgactt cgcgagtctt
     1861 ctcaacccgg gatccctgga ggttgtcccg gcatatgtgg agccgggtct tgcgggaatc
     1921 gtgcaggaaa gcaggtatca gttcgaaagg atcggctatt tctgtgtaga tacgaaggat
     1981 tcgcttccgg aaaaacccgt tttcaaccgg atcgtctccc tgcgcgactc gtgggcaaag
     2041 atcgccgccg agcctgccgg aaaatgagca cgaaagaaac gctatacagc tgcagtccaa
     2101 cgcgaagaaa agggcatgca tgaactttaa acagggtttt gacaacgaaa aataccttca
     2161 agagcaaaca cgggagatcc tggaacgcgt agaaaggttc gacaacaagc tctatcttga
     2221 atttggcgga aagctccttt acgactacca cgcggccagg gtcctgccgg gctatgatcc
     2281 caacgtcaag atgcgcctcc ttgcggaact caaggacaag gcggaccttc ttctgtgcat
     2341 atacgcgggc gatatagagc gaaagaagat acgggcggat ttcggcatca cctacgacgc
     2401 agacgccttg aagctcatcg acgagcttcg tcactggggg ataaacgtcc ttggcgtcat
     2461 catcacccgc ttcgacaacc agccggctgc ggtccatttc aggaacaagc tggagcgccg
     2521 gggcatcagg gtttacacac accgcttcac aaggggttac cccaccgatg tcgacacaat
     2581 agtcagcgac gaaggttacg gggccaatga gtatatggaa tctgacaagc ctcttgtcat
     2641 agtcaccggt ccgggaccgg gaagcggaaa gctggct
//
LOCUS       DAOPOA010000297         2674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41554_length_2674_cov_4.000382, whole genome shotgun sequence.
ACCESSION   DAOPOA010000297 DAOPOA010000000
VERSION     DAOPOA010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41554_length_2674_cov_4.000382"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKJ17_06895"
     CDS             <1..252
                     /locus_tag="PKJ17_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75736.1"
                     /translation="GVSLLYNMIAFTLKRSRIMDEIAKVNLKEFEKKPDGSWVCIQNS
                     DITMKTGKIIRVAPGTVFRKGTMFVGIDMAEALDKVPAN"
     gene            513..1256
                     /locus_tag="PKJ17_06900"
     CDS             513..1256
                     /locus_tag="PKJ17_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOD75737.1"
                     /translation="MDTFIFIVGGGFLMSAIALVGGLTTLLGPVVLERVLLPLVALAS
                     GTLLGGGFFHMIPEGGKLLSPLGAALWLMGGFSAFLALEQFLHWHHSHSKPAEAKKPV
                     TYLILIGDAVHNFLGGLGIGSTFLISPKAGVIAWIAAAAHEIPQELGDFGVLVHGGWP
                     KRRALLWNLFSALSFPLGGLIAWIASRSFNVSGLVLFGAGSFIYIAASDLIPEIKAQE
                     SLPRAILHFLFFAVGLAMMFALAYCFQRN"
     gene            1270..1437
                     /locus_tag="PKJ17_06905"
     CDS             1270..1437
                     /locus_tag="PKJ17_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75738.1"
                     /translation="MVDDDPYRSNAKQYDEWFDCEEYKYSFAGEEVKRYLSAEEWWLQ
                     KFGNDLLWAKP"
     gene            1488..2273
                     /locus_tag="PKJ17_06910"
     CDS             1488..2273
                     /locus_tag="PKJ17_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD75739.1"
                     /translation="MRISISSERVEELKEWFGDYVHTFQYSDPERQQSIDMKKWHTLR
                     VCAEISSLGTRLGLNEEELRLAEIIAMLHDVGRFEQYARYRTFVDGRSENHAELGVAT
                     IMGSGILDLFDDAVRDLIVRSVRYHNRASLPSDESETCLFYSRLVRDADKLDIWKVVT
                     DHYLRKDKDPNKAIELDLPDTPGFSEEVYHDVMNKRIVNMAHIKNLNDFKLLQIGWVF
                     DVNFQPTLDRVRECRYLEMIRETLPPSERINRIFGVIDRSCFR"
     gene            complement(2407..>2674)
                     /locus_tag="PKJ17_06915"
     CDS             complement(2407..>2674)
                     /locus_tag="PKJ17_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75740.1"
                     /translation="ADEFCRRNGFQRARNFAWDFMAPTRILATGQICNVPGRGGCGGF
                     TRITCVGGRGGGPNPPPRGGKGPGDPCQTREECRSGICLLGVCN"
BASE COUNT          567 a          726 c          788 g          593 t
ORIGIN      
        1 ggtgtttctt tactatataa tatgattgca tttaccttaa agaggagtag gattatggac
       61 gagatagcaa aagtcaatct taaggaattt gagaagaagc ccgatggctc ctgggtttgt
      121 atccaaaact ccgatataac catgaagacg ggaaagataa tcagggtagc acccggaacc
      181 gtcttcagga aaggcaccat gttcgtggga attgacatgg cggaagcgct tgacaaggtc
      241 cctgcgaact gaaggacaat taccgtttca tatcccgatg tgccgcaaac tcatagggtc
      301 ttgaactaag agcttttctc caggaccatt ttcgagccct gtttaccctc gcacggtgga
      361 cagggatagg gtgttttgtg tccttcgcat ccctgtttcc ctaacccgtt ataaaccccg
      421 cgcgtaacat cttcgcccga ttgcttcacg aggctgaggt cgtcttgaca gcaggggatg
      481 gcgagaagta ggatgtaacg tggaatcgtg agatggacac tttcattttc attgtcggag
      541 gcggattttt gatgagcgcc atcgccctgg taggtggttt gaccaccttg ctgggaccgg
      601 tggttctgga gcgtgtactt ctgccgctcg tcgctctggc gtccggcacg ttgctcgggg
      661 gcggtttctt tcacatgatc ccggagggcg gcaaactcct ttccccttta ggcgcggcat
      721 tgtggctcat gggcggcttc tcggcgtttc tcgcgctcga gcagttcctc cactggcatc
      781 attcacattc caagcccgcg gaagcgaaga agcccgtcac ctacctgatc ctcatcggag
      841 acgcggtcca caactttctt ggagggcttg gaataggaag caccttcctc atttccccga
      901 aggcgggtgt gatcgcatgg atcgcggcgg ctgcccacga gatcccgcag gagttgggtg
      961 attttggcgt gctcgtccac ggcggctggc ccaaacgtcg cgctttgctg tggaatctct
     1021 tctcggccct gagttttccc ctgggagggc tgatcgcatg gatcgcgtca cggagtttca
     1081 acgtatcggg gctggtgctc ttcggcgcgg gcagcttcat ttacattgcc gcctcggacc
     1141 tcataccgga gatcaaggcc caggaaagcc tcccgagggc gatactccat ttcctgttct
     1201 tcgccgtcgg cctcgccatg atgttcgccc tcgcatattg ttttcaacgg aattgacgtt
     1261 gtgaggcgga tggttgacga cgacccgtat cggagcaatg cgaaacagta cgacgagtgg
     1321 ttcgactgcg aggaatacaa gtattccttt gccggggaag aggtcaaaag gtacctgtcg
     1381 gccgaggaat ggtggctcca aaagttcggc aacgaccttc tgtgggcgaa accctgagaa
     1441 ccgggggagg gtcggacaag gcgaaggata acaggggaaa ccgggaaatg cggatatcga
     1501 tcagcagtga aagagttgag gaactgaagg agtggtttgg ggattacgtt cacacattcc
     1561 agtacagcga ccccgaaagg caacagagca tcgacatgaa gaaatggcac accttgcgtg
     1621 tgtgcgcgga gatatcatcc ctgggaacgc gactgggctt gaacgaggag gagctgcgtt
     1681 tggccgagat catagccatg ctccatgacg tcggccgttt tgagcaatac gcgcgctatc
     1741 gcaccttcgt ggatggaaga tctgagaacc atgccgagct aggcgtggcc acgatcatgg
     1801 ggtccgggat actggatctt ttcgacgacg cggtacgaga cctcatcgtc aggtctgtac
     1861 ggtaccacaa cagggcttcg cttccgtccg acgagtccga aacgtgcctg ttctactcaa
     1921 ggctggtgcg cgacgccgac aagctggaca tatggaaggt ggtcacggac cactacttgc
     1981 ggaaggacaa agaccccaac aaagccatcg agcttgacct gcccgacacg cccggattct
     2041 cggaagaggt ctatcatgac gtcatgaaca aaaggatagt gaacatggct catataaaaa
     2101 acctgaacga ttttaagctg cttcagatag ggtgggtctt tgacgtcaat ttccaaccca
     2161 cactggaccg tgtcagggaa tgtcgttacc ttgaaatgat ccgtgaaacc ctgccgccgt
     2221 cggagcgcat caacaggatc ttcggtgtta tcgacaggtc ctgtttcaga tgacgcgcgg
     2281 gttcgtcccg ccacccgggc agccccatga tgctgttttt ttcgaagaaa tagcgtctcc
     2341 cgtttctccg cggtgacgga gccgagggtc agaccaacgg gtctcgcccc gcgctctttc
     2401 ccggggtcaa ttgcacactc caaggaggca gatcccgctg cggcactcct ccctggtctg
     2461 gcacgggtcg cccgggccct tgcctccccg cgggggtgga ttgggtcctc ctccccggcc
     2521 gccgacgcag gtgatccggg tgaaacctcc gcagccgccc ctacccggga cgttgcagat
     2581 ctggccggtg gcgagaatgc gcgtcggcgc catgaagtcc caggcgaagt tccgggccct
     2641 ctggaagccg ttgcgccggc agaactcgtc agcc
//
LOCUS       DAOPOA010000298         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41613_length_2671_cov_2.794343, whole genome shotgun sequence.
ACCESSION   DAOPOA010000298 DAOPOA010000000
VERSION     DAOPOA010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41613_length_2671_cov_2.794343"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKJ17_06920"
     CDS             <1..374
                     /locus_tag="PKJ17_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75741.1"
                     /translation="GIYPINNPDENSPVLVTCNFSLTYFIVSGEIENSRVPSWLCIMD
                     TEGLSVMTAWAAGKFVGDLVGSFVKKSGVEEKVKHRSLIIPGYAAAILGDLEEELQGW
                     KIMIGPREAAHLPAYLKTWNS"
     gene            510..1388
                     /locus_tag="PKJ17_06925"
     CDS             510..1388
                     /locus_tag="PKJ17_06925"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOD75742.1"
                     /translation="MFLIGENLNVMSKKLGEAFLNRDAGPIRKMAEAEAEAGMDMIDL
                     NIGPARKTGPDFMEWLVGVVQEVTDLPLSLDTSNIEAIEAGLKTAKTRPLINSISARP
                     ERMEALMPMAKKYGAPFIGLTLSAEGIPRDANERGLCAAEILAAAASHGVAEEDIWID
                     PIVLPVNTQQLQIQGCTEFVMMLSDIAPSSKSTCGLSNVSNGVPVHLRGIMNRTYLMM
                     LKRYGMYSAIVDAFDNELKAIARGEMPGLEALVHRVMDGEEVDLAKLSKEEGEYIKTA
                     RVLMGQTLYSDSWLEI"
     gene            1568..1750
                     /locus_tag="PKJ17_06930"
     CDS             1568..1750
                     /locus_tag="PKJ17_06930"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOD75743.1"
                     /translation="MVINDELMRDALESSGLKTKKDAIEAGLKLLVQFNRLSEVRKFR
                     GRLKWVGDLEEMRTDR"
     gene            1747..2166
                     /locus_tag="PKJ17_06935"
     CDS             1747..2166
                     /locus_tag="PKJ17_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOD75744.1"
                     /translation="MILVDTSVWVDYFNGAVNPQTDWLDAALGTEPVVMGDLILAEIL
                     QGFQNDRDYRKAQRLLEGIPFMDMLGRELAVKSAQHYRYLRKKGVTVRKTIDVMIGTF
                     CIHHGITLLHRDRDFVPMEKYLNLMSVVPTNLEEYDE"
     gene            2159..2671
                     /locus_tag="PKJ17_06940"
                     /pseudo
     CDS             2159..2671
                     /locus_tag="PKJ17_06940"
                     /inference="COORDINATES: protein motif:HMM:NF023165.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
BASE COUNT          645 a          673 c          813 g          540 t
ORIGIN      
        1 aaggcatcta ccccatcaac aaccctgacg agaactcacc ggtcctcgtg acgtgcaatt
       61 tctcgctcac ctacttcata gtaagcggag agatcgagaa ctcgcgcgtg ccaagctggc
      121 tgtgtataat ggacacggaa gggctttctg tcatgacggc ctgggcggcg ggcaagttcg
      181 tcggtgacct ggtcggttcg tttgtaaaga agtccggcgt ggaggaaaag gtgaaacacc
      241 gcagcctcat catccccggc tacgccgccg cgatactggg agatctggaa gaggaactgc
      301 aaggctggaa gatcatgata ggcccgcggg aggcggcaca cctgccggcg tatctgaaga
      361 cgtggaattc atgaatgcgc atacgggtct ttcgcttaag cgaaaaacgg gcataggagc
      421 acacggggaa aagacacagt tttttcctgc tttatctcgt atgcccgtct gcccgtaggc
      481 ccgtaagcaa agatcaaaga gaggccgtca tgtttctcat aggtgaaaac ctcaacgtta
      541 tgtcgaagaa actcggtgag gcgttcctga acagggatgc cgggccgata cggaagatgg
      601 ccgaggccga ggccgaggcc ggaatggaca tgatcgacct caatatcggg cccgcgcgta
      661 agaccggccc cgatttcatg gaatggcttg tcggtgtcgt gcaggaggtc acggaccttc
      721 ccctgtctct cgacacgtcc aatatagagg caatagaggc gggcctgaag acggcaaaga
      781 caaggccgct catcaactcc atctcggcgc ggccggaaag gatggaggcc ttgatgccca
      841 tggcgaagaa gtacggcgcg cctttcatcg gccttacact cagcgccgag ggaattcccc
      901 gcgatgccaa cgaacggggg ctctgtgccg ccgagatcct ggcggctgcc gcgagccacg
      961 gtgtcgcgga ggaagacatc tggatcgacc ccatcgtcct gcccgtgaac acccagcagc
     1021 tccagatcca gggttgtacc gagtttgtca tgatgttgtc cgacattgca ccttcctcta
     1081 agtccacctg cggcctgtcg aacgtgtcaa acggggtgcc ggtgcatctg agaggtatca
     1141 tgaacaggac ctatctcatg atgctgaagc gctacggcat gtattccgcc atcgtcgacg
     1201 ccttcgacaa tgaactcaag gccatagccc ggggagagat gcccgggctg gaagccctcg
     1261 tccaccgcgt catggacggc gaagaggtgg acctcgcaaa gctctcgaag gaggaaggcg
     1321 agtacatcaa gactgcccgg gtcctcatgg gtcagacgct ctattccgat tcgtggctgg
     1381 agatatgaaa aacgacttga gcggttcgaa ttgcttgaat cgcttgagag gccgtcggta
     1441 aagtcttttt tctacgtcat gcaggccttg atcggaatga aatgagacgg gtcctgtttg
     1501 aggaaacttt cagaaatgtg tataataacg caatgaatac acataagggg cggatatgag
     1561 gacgaatgtg gtcataaatg atgagttgat gagagatgcc ctcgagtcgt cgggtctgaa
     1621 gacgaagaag gatgccatcg aggccggatt gaaactgctc gtgcagttca accgcttgtc
     1681 ggaggttcga aagttccgcg gcaggttgaa atgggtggga gatctggaag agatgcgcac
     1741 agacagatga tccttgtcga cacatcggtc tgggtggatt atttcaacgg ggcagtgaac
     1801 ccacaaaccg actggcttga tgcagccctg ggcacggaac ccgttgtaat gggtgatctc
     1861 attctggccg agatactgca gggatttcag aatgacagag attaccggaa ggcacagagg
     1921 ctcctcgaag gcattccttt catggatatg ctggggaggg aactcgcggt aaagagcgca
     1981 caacattaca ggtacctgag aaaaaagggt gtaactgtcc ggaagacgat agatgttatg
     2041 atagggactt tctgcatcca ccacggcatt acgctcctgc accgcgacag ggactttgtg
     2101 cccatggaga aatatctgaa cttgatgtcg gttgttccta caaatctgga ggagtatgat
     2161 gaatagagcg tttagacgga cattgatatg ggtacttgtg gcgtcccttg tcattcctgt
     2221 gggggtgttc gcgcagggta cggggacgtc gaagccggcg ttcaagcagg aggagttgga
     2281 gcagctcctg gctcccatcg ccctttatcc cgacgacctc gtagcccaga tactcatggc
     2341 gtcgacgtac ccctcgaggt ggttgaggcg gcgcggtggg tgaaggcgaa ccccaatctt
     2401 aaggggaatc agttgacatc cgccctggag aaacagaact gggaccccag cgtcaaatcc
     2461 cttgtgaact ttccgtcggt cctcaccatg atgaatgaga aattggactg gacgcagaag
     2521 cttggggacg cgttcctggc acagcagaag gatgtcatga acacggtcca gaagctccgg
     2581 gcaaaggcgc aggcctcggg gaacctcaag acgacgaagg aacagaaggt cgtcgtgcag
     2641 gaccaggtca tcgtgataca gtcggccgac c
//
LOCUS       DAOPOA010000299         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41617_length_2671_cov_2.133792, whole genome shotgun sequence.
ACCESSION   DAOPOA010000299 DAOPOA010000000
VERSION     DAOPOA010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41617_length_2671_cov_2.133792"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKJ17_06945"
     CDS             <1..346
                     /locus_tag="PKJ17_06945"
                     /inference="COORDINATES: protein motif:HMM:NF019122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HOD75745.1"
                     /translation="AEIKRLGLAMGARETTFMGLSGMGDLILTSYGSLSRNRIFGMEL
                     AKGRSPGEIIASQKTVVEGYYTINAARNLAARLGVVMPITEELYRIVYEDKDITASLK
                     DIITREFKKEDY"
     gene            complement(417..1661)
                     /locus_tag="PKJ17_06950"
     CDS             complement(417..1661)
                     /locus_tag="PKJ17_06950"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD75746.1"
                     /translation="MKTSLFDRKIFSWCLFDFANSSYSAVIAAVIFPVYYTTVVVGND
                     ANLGDLWWGRAISVSMAFVALSSPFLGGISDYARAKKKFLVAYTLLCVLATAFLALVD
                     RGMVMAGFVLLVLANVGMEGGIVFYNSFLNDIADNEHQGRVSAWGYATGYLGSIISLA
                     VALILVKTDHITFVWPMVAVFFVVFSLPAFIFLPADQGKGQKLSKAARDGFATTWGVL
                     KRMWSDRDQRRFLLAYFLYEDGVNTVIVFSSIFASTTLGFTSEQLIGLYLVVQVTALA
                     GAFLMARPIDYWGPKKVVSLSLILWMVVTIVVYFIYSKAFFFVAASMAGLGLGTIQAA
                     TRAFFAQFIPPGDESKYFGVYSLVGKTSAVVGPLIFGTVSSMTNNQRPAVAAISVLFI
                     TGCIILQTVRGGKANVKKTGNG"
     gene            1727..2242
                     /locus_tag="PKJ17_06955"
     CDS             1727..2242
                     /locus_tag="PKJ17_06955"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HOD75747.1"
                     /translation="MMDLSVFDTMKEDELRDYLTFLLWHYRLMDAFWFIYVSDTFGQE
                     QAERINEKVWSRVPEMGTRDLLQRFGITEKGLKGFTKVLKLFPWTILVEYKFEEKDSE
                     VIVTVPHCPTQEARLKRGLGEFVCKHMHHGEFESIARAVDPSIKVECLFAPPDEHPED
                     CFCKWRFTEAS"
     gene            2284..>2671
                     /locus_tag="PKJ17_06960"
     CDS             2284..>2671
                     /locus_tag="PKJ17_06960"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75748.1"
                     /translation="MIIGVPKEIKEGENRVSMTPAGVHTLVSAGHHILFEEGAGVGSG
                     LTDEEYAREGAEIVESKRTVYARSDMIVKVKEPLDTELSLLQEGQTLFTYLHLASSEK
                     LTNGLVKKKVTAVAYETIQSPDGRLPL"
BASE COUNT          661 a          750 c          743 g          517 t
ORIGIN      
        1 cgcggagata aaacgccttg gcctcgccat gggtgcccgc gaaacaacct tcatgggcct
       61 gtccggcatg ggcgatctca tcctcacctc atacgggagc ctcagcagga acaggatctt
      121 cggaatggaa ctcgccaaag gaaggagccc gggggagatc atcgcctctc aaaagaccgt
      181 cgtcgaaggc tattacacga tcaacgcggc ccgcaacctc gccgccaggc tcggcgttgt
      241 aatgcccatc accgaggaac tctaccgcat agtctacgag gacaaggaca tcaccgcatc
      301 cctgaaagac atcatcacga gggaattcaa gaaagaggac tactgagaag acaggtaata
      361 ggtaataggt gccgggttat gggaagacag aagagacccc tcccgcctat tacctattac
      421 ccattacctg tcttcttcac attcgccttt ccgccccgca ccgtttggag gatgatgcaa
      481 cccgttatga agagcacgga tattgccgcc acggccgggc gctggttgtt tgtcatcgag
      541 gacacggtcc cgaagatgag ggggccgacg accgcggacg ttttccccac aaggctgtac
      601 acgccgaagt acttggattc gtctcccgga gggatgaact gggcgaaaaa ggcgcgtgtc
      661 gcggcctgga tcgtgccgag accgaggccg gccatggacg ccgccacgaa gaagaaggcc
      721 ttgctataga tgaaatagac cactatggtg acaaccatcc atagtatgag ggacagcgag
      781 acgacctttt tcggccccca gtagtctatc ggccgcgcca tgaggaacgc acccgccagg
      841 gccgtcacct ggacgacgag atagagccct ataagctgtt ccgacgtgaa cccgagtgtt
      901 gtggaagcaa agatactcga aaagacgatc accgtgttga ccccgtcctc gtacaggaag
      961 taggccagaa ggaacctgcg ctggtccctg tccgaccaca tccttttcaa gaccccccat
     1021 gtcgtggcaa aaccatcccg ggccgccttt gacagcttct gtccctttcc ctggtcggca
     1081 ggaaggaaga tgaaggccgg gagggagaac acgacaaaga agaccgccac catgggccag
     1141 acaaaggtga tatggtccgt cttcaccaga atgagggcga cggcgagtga gattatcgac
     1201 ccgagataac ccgtggcgta tccccaggcc gataccctcc cctgatgttc gttgtcggct
     1261 atgtcattga ggaaggaatt gtagaatact atcccgccct ccatacccac attggcaagt
     1321 acgagcagaa cgaaccctgc catcaccatt cccctgtcca ccaaagccag aaacgcggta
     1381 gcgaggacgc acagcagcgt gtaggcgacg agaaacttct tcttcgccct tgcgtaatcc
     1441 gagatccctc cgaggaacgg ggagctcaag gcaacaaagg ccatgctcac cgatatggcc
     1501 cttccccacc acaggtcacc caggttcgcg tcgttcccga ccaccaccgt cgtgtagtac
     1561 acgggaaata ttaccgccgc gatgaccgcg gagtaacttg agttcgcgaa gtcaaagaga
     1621 caccacgaga atatttttct gtcaaaaagg ctcgtcttca ttgtcttttg gtgttgaact
     1681 aatttacaat aaacgttgtc gtgattcact aaaattgagg aggaacatga tggacctttc
     1741 cgtttttgac actatgaagg aagacgaact gcgggactac ctgaccttcc tcctgtggca
     1801 ttatcgtctt atggacgcct tctggttcat atatgtcagt gataccttcg gccaggaaca
     1861 ggctgagcgc attaacgaaa aggtctggtc ccgcgtgccc gaaatgggaa cccgcgacct
     1921 gctgcagcgc ttcggcatca ccgaaaaggg cctcaagggt tttacaaagg tgctgaagct
     1981 ctttccgtgg acgatcctcg tcgagtacaa gttcgaggag aaggactcag aggtcatcgt
     2041 taccgttccc cactgcccga cgcaggaggc gcgcctgaaa cggggccttg gggagtttgt
     2101 ctgcaagcac atgcatcacg gggagtttga gagcatagcc cgggccgtgg acccttcaat
     2161 aaaggtggaa tgcctcttcg cccctcccga cgagcatcca gaggattgct tctgtaaatg
     2221 gcggttcacg gaagcctcgt gaatagagcc tgtcgtcgaa acgctatctg atatggagga
     2281 agaatgatca taggagtacc gaaagagatc aaggaaggcg agaacagggt ttccatgaca
     2341 ccggcgggcg tccacaccct cgtctccgcg ggtcatcaca tacttttcga agagggagcg
     2401 ggggtgggaa gcggtctcac cgacgaggaa tacgcgcggg aaggggccga gatcgtcgaa
     2461 tcgaagagaa cggtctatgc cagatcggat atgatcgtga aggtcaagga gcctctcgac
     2521 actgagcttt cccttcttca ggagggacaa acactcttca cctatcttca cctggcctcg
     2581 tcggagaaac tgacgaatgg gcttgttaag aaaaaggtga cggccgtggc ctacgagacc
     2641 atccagtcgc cggacggccg cctgccgctg c
//
LOCUS       DAOPOA010000300         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41634_length_2670_cov_3.521989, whole genome shotgun sequence.
ACCESSION   DAOPOA010000300 DAOPOA010000000
VERSION     DAOPOA010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41634_length_2670_cov_3.521989"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKJ17_06965"
     CDS             complement(<1..361)
                     /locus_tag="PKJ17_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD75749.1"
                     /translation="MAETILVTGGCGFIGTNFVRHMLERYDYTIVNLDKLTYAGNLEN
                     LEDVAGDGRYTFVRGDIASMADVEGVFERPIDIVVNFAAESHVDRSIMDPDAFIRTNI
                     NGTFNLLELARKKGVGRF"
     gene            complement(354..809)
                     /locus_tag="PKJ17_06970"
     CDS             complement(354..809)
                     /locus_tag="PKJ17_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOD75750.1"
                     /translation="MIDGVAIKMLKVIPDERGRLMEILRSDDEIFTQFGQVYMTTTYP
                     GVVKAWHYHRKQDDLIACVRGMIKLVLYDDREGSPTRGEVNELFVGDHDPRLVRVPRM
                     VYHGWKCVSLEEAIIINAPTMVYDYVGPDEFRIDPHENDIPYTWERKDG"
     gene            complement(806..1552)
                     /locus_tag="PKJ17_06975"
     CDS             complement(806..1552)
                     /locus_tag="PKJ17_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD75751.1"
                     /translation="MKGIILAGGLGSRLAPLTRITNKHLLPVYDRPMIYYPIETLINA
                     GITDIMIVTGGNHAGDFLRLMGNGKDFGLKHLNYTYQEGEGGIADALSLAEYFADGEP
                     VCVVLGDNIIERNIIKAVQDFEKQGAGAKIMLKEVPDPERFGVAEITDGRLVNIVEKP
                     KSPRSNLAVIGIYLYDATVFDIVKTLKPSERGELEITDVNNAYVRNGTMTWEMLDGWW
                     TDAGTFESLLRASVLVSQTGANNVAPGEKL"
     gene            complement(1569..>2670)
                     /gene="lptD"
                     /locus_tag="PKJ17_06980"
     CDS             complement(1569..>2670)
                     /gene="lptD"
                     /locus_tag="PKJ17_06980"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOD75752.1"
                     /translation="SESQLKSTIFVEKPLPRSLLTGGVTHFRNLLVKDNDTLFQYYPE
                     VTYFTEYIPVFGGKMYLDLNSSLTNFYRDSGGTYTRLAVDPSLRLPYSFKGLNTLLSG
                     TLYETGYAVNRSEDTGGNSNYRQTFRLDADANMQFMRGYNTGWSWLESMQSVVRPNVR
                     YTYIPGTGYKDAPKIDAYDRINQTNAITYSINHYLYDTSGETGFRREMSFFEVSQTYG
                     ISGHLEESELYKGSGSRFSDIDLKFTLSPLDHLSFAHESVFSVHGEGAKTLRNTISYL
                     VPGVYSAIVAHNYNSGLNNDIFVEMFASYWVFEGGYNIRYTFMDKDWIDTEYRLKYKP
                     GCWSVVLSLSQSKRPRDTSFKLSFDLTGITSR"
BASE COUNT          496 a          766 c          786 g          622 t
ORIGIN      
        1 cgaagcgacc gacgcccttc ttccgcgcca gctccaggag gttgaaggtg ccgtttatgt
       61 tcgtcctgat gaaggcgtcg gggtccatga tgctcctgtc gacgtgggac tccgccgcga
      121 agttgacgac gatgtcgatg ggcctttcga agacgccctc gacgtcggcc atggacgcga
      181 tatccccccg cacgaaggtg tacctcccgt ccccggcgac gtcctcgagg ttctcaaggt
      241 tgcccgcgta cgtcagcttg tcgagattga cgatggtgta atcgtatctt tccagcatgt
      301 ggcggacgaa gttggtcccg atgaagccgc agccgccggt gacgaggatc gtttcagcca
      361 tcttttctct cccaggtgta gggtatgtcg ttctcgtggg ggtctatcct gaactcgtcg
      421 ggcccgacgt aatcgtagac catggtgggg gcgtttatga tgatggcctc ctcgaggctg
      481 acgcacttcc acccgtgata gaccatgcgc ggcaccctga cgagccgggg gtcatggtcg
      541 ccgacaaaga gctcgttgac ctctccccgg gtgggcgatc cctcgcggtc gtcgtagagg
      601 acgagcttta tcatgcccct cacgcaggcg atgaggtcgt cctgcttcct gtggtaatgc
      661 cacgccttca ccaccccggg gtatgtggtc gtcatgtaga cctggccgaa ctgcgtgaag
      721 atctcatcgt cgctgcggag gatctccatc agcctgcccc tctcgtcggg gatcaccttc
      781 agcatcttga tggccacgcc gtctatcata gtttctctcc cggggccacg ttgttggccc
      841 ccgtctgtga aacgagcacg ctggcacgga gcagggattc aaaggtgccc gcgtccgtcc
      901 accagccatc gagcatctcc caggtcatcg tgccgttcct tacgtaggcg ttgttcacat
      961 cggtgatctc aagctcgccc cgctcggacg gcttaagggt ctttacgata tcaaaaacgg
     1021 tcgcgtcgta gaggtagatg ccgatgacgg caaggttgct ccggggactc ttcggcttct
     1081 ccacgatgtt gacaagcctg ccgtccgtaa tctcggcgac gccgaatctt tccggatcgg
     1141 ggacctcctt gagcatgatc ttggcccctg ccccctgttt ctcgaagtcc tggacggcct
     1201 tgatgatgtt cctttcgatt atgttgtccc cgaggacgac gcagaccggt tctccgtccg
     1261 cgaaatattc cgccagtgac agggcgtcgg cgatgccgcc ctccccttcc tggtacgtgt
     1321 agttgagatg cttgagcccg aagtcctttc cgttgcccat gaggcgcagg aaatcaccgg
     1381 cgtgattgcc gcccgtcacg atcatgatgt ccgttatgcc cgcgttgatg agggtctcta
     1441 tgggatagta gatcatgggc ctgtcgtaca cgggcagaag atgcttgttc gttatccggg
     1501 tgaggggcgc gagacgcgac cccagaccgc ctgccagtat gatacccttc atgtgaaccc
     1561 tcctccttct accgcgatgt gatgccggtg agatcgaagg agagcttgaa gctcgtgtcc
     1621 ctcggcctct tggactgcga cagtgacaac accacggacc agcagcccgg tttgtatttc
     1681 aggcgatact ccgtgtcgat ccagtccttg tccataaacg tgtaccgtat gttgtatcct
     1741 ccttcaaaga cccagtacga ggcgaacatc tccacgaaga tatcgttgtt gagccccgaa
     1801 ttgtagttgt gcgccacaat ggcgctgtac accccgggga caaggtacga tatggtgttc
     1861 ctcaacgtct tcgcgccttc gccgtggacg ctgaaaacac tctcgtgcgc gaagctgaga
     1921 tggtccagcg gcgacagggt gaatttcagg tcgatgtcgg aaaagcgcga acccgaacct
     1981 ttgtagagct cggactcctc gagatggccc gagatgccat aggtctggga cacctcgaaa
     2041 aaggacatct cgcgtctgaa accggtctcc ccagaagtgt cataaagata atggttgatc
     2101 gaataggtta tcgcgttcgt ctggtttatc ctgtcgtagg catctatctt gggtgcgtcc
     2161 ttgtagcccg tgccggggat gtatgtgtac cggacattcg gtcttaccac gctctgcatg
     2221 gattccagcc aggaccagcc ggtattgtag ccccgcatga actgcatgtt ggcgtcagcg
     2281 tcgaggcgaa aggtctggcg gtagttgctg ttgcctcccg tgtcctcgct gcggttgacg
     2341 gcgtagccgg tctcgtagag ggtaccgctg agaagcgtgt tcagaccctt gaaggagtat
     2401 ggcagtctca atgacgggtc gacggcaagg cgcgtatagg tccctcccga gtcgcgatag
     2461 aaattggtca gagagctgtt gaggtcgagg tacatctttc caccgaacac aggtatgtat
     2521 tcggtgaagt acgtcacctc gggataatat tggaacaacg tgtcgttgtc cttcacgaga
     2581 aggttcctga agtgtgtgac cccgccggta aggagggacc ggggcagggg cttctccacg
     2641 aagatcgtcg acttgagctg actctcgctc
//
LOCUS       DAOPOA010000301         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41744_length_2664_cov_4.536221, whole genome shotgun sequence.
ACCESSION   DAOPOA010000301 DAOPOA010000000
VERSION     DAOPOA010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41744_length_2664_cov_4.536221"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKJ17_06985"
     CDS             complement(<1..296)
                     /locus_tag="PKJ17_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOD75753.1"
                     /translation="MKRVVAVMMFCVSFILCAFALAQNPQAADADRSFETIKAKGTIV
                     LGLDASFPPMGFREKGSCEIVGFDIDLAREAARRLDLTITPKAVRWNRIIPALNG"
     gene            450..1253
                     /locus_tag="PKJ17_06990"
     CDS             450..1253
                     /locus_tag="PKJ17_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOD75754.1"
                     /translation="MMHRSETMMERRAGAAGKRLLIVFVLLSAMLAGGQAEAQNPFTS
                     TAYDPVFIGEKSEIEIGKNTDEQLRQKYRISTDRQLNQRINAIGQKLAARSERSNLAY
                     SFTVIDDELVNAFAAPGGYIYITTGILKKLKSEHEIAGVLGHEIGHVVHKHSLKALQR
                     QMLAQFGLSIMGAMLGDGGLSGSLIVKASEISTSLLLLKNSRENELQADAEGVRIMHA
                     AGYNPRAMIDIQRMLLKEAGGKNPPAIISTHPPSQERIDAIEQAIRNMP"
     gene            complement(1260..1844)
                     /locus_tag="PKJ17_06995"
     CDS             complement(1260..1844)
                     /locus_tag="PKJ17_06995"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HOD75755.1"
                     /translation="MSTFLKRTIVMFAAVALIFPFLADESFAAKKTKDQQRKEIRAMR
                     DKALSKLYRLEPGSKKAIAGAAGYGVFDLFSLKILVTGSTNGRGIVVDNASRKETFMK
                     MYRVSAGLGVGAKDFRWVFVFHSKPALNDFINKGWEFGAEAGAAAKDRKRGGALGNAV
                     SAGPDISVYQLTEKGLALEADIGGSKYWKDKDLN"
     gene            2026..>2664
                     /locus_tag="PKJ17_07000"
     CDS             2026..>2664
                     /locus_tag="PKJ17_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOD75756.1"
                     /translation="MKENYTVSRYILSRLREIGVKHLFGVPGDYVLDFLDAVIDSPIE
                     WVGTCNELNAGYAADGYARTNGIGAAVVTYGVGGLSILNAAAGAFAEHVPLIVISGAP
                     PAGRRESGALVHHLVSNYYLQLEIFKKVTVDATILTDPNTAPEEIDRVLGQCVSRRLP
                     VYLEIPVDITQAPCRAPGVFPGAAPPVSDEDSLTECVSEIVEMLDNAEHPLML"
BASE COUNT          540 a          816 c          793 g          515 t
ORIGIN      
        1 ccgttcaggg cgggtatgat cctgttccag cgaacggcct tcggagtgat ggtgaggtcg
       61 agccgccggg ccgcctccct ggcaagatcg atgtcgaagc cgacgatctc gcagctgccc
      121 ttctctctga accccatggg cggaaaagag gcatcgagac ctaggacgat ggtgcctttc
      181 gcctttatcg tctcgaacga ccggtcggcg tccgccgcct gcgggttctg cgccaatgcg
      241 aaggcgcaca ggatgaagct cacacagaac atcatgacag cgacaaccct tttcatggat
      301 ccttcccctc cggttgtggt ggatgcttgc taacggttcc agtatatccg acggcgacgt
      361 gctttacaag ggctgggacg gttgccgccg gcgcttccat gtgttatctc tattacatga
      421 taggaatgtc attgaggaag gatggaagaa tgatgcatcg ttcggagaca atgatggaaa
      481 ggcgtgccgg cgctgccggg aagaggctgc tcatcgtctt cgtcctcctg tccgccatgc
      541 tggcgggcgg tcaggccgag gcccaaaatc ccttcaccag caccgcgtac gaccccgtct
      601 ttatcggtga gaaaagcgag atcgagatcg ggaagaacac ggacgagcag ttgaggcaaa
      661 agtacaggat atcgactgac cgccagctca accagcggat aaacgcgatc ggccagaaac
      721 tcgcggcccg ttccgagagg agtaacctgg cgtacagctt caccgtcatc gacgacgagt
      781 tggtcaacgc cttcgcggcg ccgggcggat acatttacat cacaacgggc atattgaaga
      841 aactgaagag cgaacacgag atcgccggcg tgctcggcca cgagataggc cacgtcgtcc
      901 acaaacactc cctgaaagcg cttcagcgtc agatgctggc ccagttcggg ctttcgatca
      961 tgggagccat gctcggggac ggaggcctct ctgggtcgct catcgtcaaa gcgtcggaga
     1021 taagcacgag ccttttgctc ctcaagaact cgcgggagaa cgagcttcag gccgatgccg
     1081 aaggggtgag gatcatgcac gccgccggct acaacccgcg ggccatgatc gacatccaga
     1141 ggatgctcct gaaggaggcc gggggcaaga accctcccgc gatcatctcc actcatccac
     1201 cctcacagga aaggatcgac gccatcgaac aggcgatacg gaacatgccg tagtttcagt
     1261 cagttcaggt ccttgtcctt ccagtacttg cttccgccga tgtccgcttc gagggcaaga
     1321 cccttttccg tgagttgata gacggagatg tccggtcccg cggacaccgc gttgccaagg
     1381 gcgccgcctc tcttcctgtc cttcgccgcc gcgcccgcct cggccccgaa ttcccagccc
     1441 ttgttgatga aatcgttgag ggccggcttg ctgtggaaga cgaacaccca ccggaagtcc
     1501 ttggctccga cgccgagccc cgcgctcacc cggtacatct tcatgaaggt ctcttttctc
     1561 gacgcattgt cgacgacgat acccctgccg tttgtcgaac cggtgacaag gatcttcagg
     1621 ctgaagaggt cgaatacccc atagccggca gcccccgcta ttgccttctt cgatccaggc
     1681 tccaggcgat agagcttcga gagagccttg tccctcatcg cgcgtatctc ctttctctgc
     1741 tggtccttcg tttttttcgc ggcgaaggac tcgtcggcga ggaaaggaaa gatcagggcc
     1801 acggccgcga acatcacgat ggtgcgcttc aggaaggtgc tcatggtctt ctcctttgcg
     1861 atgggatctc gaggggcccg tgcccctctc acagtatagg gcggttacgc gggtccctgt
     1921 caacaggaga aggcccatcg cgcccggcca ttgactttcc cgatgccatc gtgttgcact
     1981 tatacggtgc agccgcacac acacaacgcc aggggggtca tatcaatgaa agagaattac
     2041 actgtgtcac ggtacattct cagccgtctg cgggaaatag gtgtcaaaca cctcttcgga
     2101 gtgcccgggg attacgtcct cgatttcctc gacgcggtga tcgacagccc catcgaatgg
     2161 gtggggacct gcaacgagct caacgcgggt tacgcggcgg acgggtatgc ccggacgaac
     2221 ggtatcgggg ccgccgtggt gacgtacggg gtgggaggcc tgagtatcct caacgccgcg
     2281 gcaggggcat tcgcggaaca cgtgcccctc atcgtcatca gcggggcccc gccggcgggg
     2341 cgcagggagt cgggcgccct ggtgcatcac ctcgtctcca attactacct ccaactggag
     2401 atattcaaga aggtcacggt cgacgcgacc atactgacgg accccaacac cgcccccgag
     2461 gagatagacc gtgtgctcgg acaatgcgtc agccggagac tgcccgtcta cctggagatc
     2521 cccgtggaca ttacgcaggc cccctgccgg gccccggggg tctttcccgg cgcggcgccc
     2581 cccgtctccg acgaggacag tttgacggaa tgcgtgtcgg agatagtgga gatgcttgac
     2641 aacgccgagc atcccctgat gctg
//
LOCUS       DAOPOA010000302         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41848_length_2660_cov_2.378503, whole genome shotgun sequence.
ACCESSION   DAOPOA010000302 DAOPOA010000000
VERSION     DAOPOA010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41848_length_2660_cov_2.378503"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..2560
                     /locus_tag="PKJ17_07005"
     CDS             206..2560
                     /locus_tag="PKJ17_07005"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOD75757.1"
                     /translation="MSLKEYKPGTAFPGVIGRTFDVSEPAWPKPLRAKEGAPNVLFIV
                     LDDTGFGQLGCYGSPIETPNLDSLAANGLIYSNMHTTALCSPTRSCILTGRNHHSNAM
                     ACITEGSTGYPGSNGNIPFENGFLSEILSQHGYSTYAIGKWHLTPADQISAAGPYDRW
                     PLGRGFERFYGFLGGETHQYYPELVYDNHQVEPPKTPEEGYHVTEDLVDKAIAFIADA
                     KQVAPNKPFFLYFCTGAMHAPHHVPKQWADRYKGKFDEGWEVYREQVFAKQKNLGIVP
                     ADAEISRHDPDVKPWKDCSADEKRLYARMMEVFAGFLTHTDHHVGRLLEFLKRINEFD
                     NTLIMVISDNGASSEGGPTGSVNENLFFNNVPESLEENLKEIDKLGGPDTFNHYAWGW
                     TWAGDTPFRRWKRETYRGGISDPFIVHWPEGIKAKGEVRTQYAHAIDMVPTVLESLGI
                     GAPEEVRGVTQSPIEGVSFAHTFDSADASTRHVTQYFEMMGHRSLYHDGWRAVCPWPG
                     PSFTEAGKFFGTPIPKGTLTELDANNWELYHVEKDWTENHNIASENRAKLIEMVGTWY
                     VEAGKYKVMPVDGRGTQRFAEERPQIAKDRTSYTFYPGTQSVPYNAGPRLLNRTHSIT
                     ADVEVPEKGAEGVLISFGGTDGGYALYLMDGKLQYVQNYVAREYLHVASTEPVPPGHH
                     QLRFEFEATGGPDIGQGRGSPGHVELYMDGKLVGAADFALTTPLILGLTGGIKVGADP
                     GAPVAPFYDAPFEFTGTICSVTIDVSGEVIKDDEAELRAIMARQ"
BASE COUNT          597 a          805 c          794 g          464 t
ORIGIN      
        1 caacctcgcc cgcaatgccg atggatcggt agacctctac tttggaccaa aggcgccacc
       61 aggataggag gcaaactggg tccagaccgt cccgcataaa ggctggttta ctatcctcag
      121 gctctacggc cctctggatc cctggttcga taaaacgtgg cggccgggtg agatcaaacc
      181 aacaaactaa aaggaggaaa tcgtcatgtc gctgaaggag tataaaccgg ggacagcatt
      241 ccccggcgtg atcggtcgta ccttcgacgt ttcggagccg gcatggccca aacccctcag
      301 ggcgaaggag ggagcaccta acgtcctgtt catcgtcctc gatgataccg gtttcgggca
      361 gcttggctgc tacggcagtc ctatcgaaac gccaaatctc gacagccttg cggcgaatgg
      421 tctcatctac agcaatatgc acaccacagc gctctgttcg cccacgcgtt cctgtatcct
      481 cacaggccgc aaccaccact ccaacgctat ggcctgcatc acggagggtt ccaccggtta
      541 tccgggctcc aacgggaaca ttcccttcga gaacgggttc ctctcggaga tcctgtcgca
      601 gcacggttac agcacctatg ccatcggcaa gtggcacctc acccccgccg atcagatctc
      661 ggccgccggc ccctatgacc gctggcccct gggtcgcggc ttcgagcgtt tctacgggtt
      721 tctcggagga gagacacacc agtactaccc ggaactcgtc tatgacaacc accaggtgga
      781 gccgccgaaa acaccggagg agggttatca cgtaaccgag gacctggtag acaaggcgat
      841 cgcattcata gcggacgcca agcaggtcgc ccccaacaag cccttcttcc tgtacttctg
      901 cacgggggcg atgcacgcgc cccaccacgt gcctaagcag tgggcggacc gctataaggg
      961 aaagttcgac gaagggtggg aagtgtaccg cgaacaggtg ttcgcgaaac agaagaacct
     1021 gggcatcgta cccgcggacg cggagatctc gcgtcacgat ccggatgtca agccctggaa
     1081 ggactgctcg gcggatgaga aaaggctcta cgcccggatg atggaggtct ttgcgggttt
     1141 cctcacccat accgaccacc acgtaggccg gctcctggaa ttcctgaagc gcatcaatga
     1201 attcgacaac acactcatca tggtcatctc ggacaacggg gccagttccg agggcgggcc
     1261 gacgggctcc gtcaacgaga acctcttctt caacaacgtt cccgaatccc tcgaggagaa
     1321 cctcaaagag atcgacaagc tgggaggccc cgacactttc aaccactatg cctggggctg
     1381 gacctgggca ggcgataccc ctttccggcg ctggaagcgc gaaacctatc gcggaggcat
     1441 cagcgatccc ttcatcgtgc actggcccga gggcataaag gcaaaaggag aggtgcggac
     1501 gcagtatgcc catgccatcg acatggtccc cacagtcttg gaatcgctgg gcatcggggc
     1561 accggaagag gttcggggag tgactcagtc ccccatcgag ggagtaagct tcgcccacac
     1621 gttcgatagc gcggacgctt ccactaggca cgtcacgcaa tacttcgaga tgatggggca
     1681 ccgctccctc tatcacgacg gatggcgggc cgtctgcccc tggcccgggc cttcgttcac
     1741 ggaagcaggc aagttcttcg ggaccccgat accgaaaggg actctgaccg aactggatgc
     1801 caacaactgg gaactgtatc acgtcgagaa ggactggacg gaaaaccata acattgcctc
     1861 ggagaacagg gccaaactca tcgaaatggt gggtacctgg tacgtggaag caggcaagta
     1921 caaggtgatg ccagtcgacg gccgcggcac acaacgcttc gcagaggagc gccctcagat
     1981 cgccaaagac cgcacgagct acacgttcta tcccggcacc cagtccgtcc cgtacaatgc
     2041 gggaccccgc ctcctcaacc ggacccacag catcacggcc gacgtggagg tccccgaaaa
     2101 aggggcggag ggagtgttga tctccttcgg cgggacggac ggtggttacg ctctgtatct
     2161 catggacggc aagctgcagt acgtgcagaa ttacgtagcc cgtgaatatc tccacgtagc
     2221 ctctaccgag ccggtacccc cggggcacca ccaactgcgt tttgagttcg aggccacagg
     2281 gggccctgat atagggcagg gcaggggctc gcccggacac gtcgagcttt acatggacgg
     2341 caaacttgtc ggcgcagccg acttcgcgct gaccactccg cttatccttg ggctgacggg
     2401 gggcatcaag gtcggcgccg acccgggagc acctgttgca cctttctatg acgcgccgtt
     2461 tgagttcacg ggcacgattt gcagcgtgac catcgacgtc agcggagagg tcataaagga
     2521 cgatgaagcg gaactgaggg cgatcatggc gcggcagtga cggctgaagg agacacgacc
     2581 gtgcagagac cgcaggcgtg gatggaaagg gaggggttga agcgaggcgg ctggtgcttt
     2641 gtgatcgcgt ggacggtttg
//
LOCUS       DAOPOA010000303         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41902_length_2657_cov_3.488086, whole genome shotgun sequence.
ACCESSION   DAOPOA010000303 DAOPOA010000000
VERSION     DAOPOA010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41902_length_2657_cov_3.488086"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PKJ17_07010"
     CDS             complement(<1..213)
                     /locus_tag="PKJ17_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75758.1"
                     /translation="MTESVSQKETVLPVLERTFSAFDRAMDELRPRLTVEEIGVLTFV
                     SKGIARVKGLPTVRSGELLLFSGNIQG"
     gene            complement(210..1013)
                     /locus_tag="PKJ17_07015"
     CDS             complement(210..1013)
                     /locus_tag="PKJ17_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75759.1"
                     /translation="MLIDWFTVVAQIVNFLVLVALLKYFLYGRVINAMDKREEKIVSR
                     LTDAEEKERRAEEEARSYQEKSREVEEKRGELLARVEEEADGKRKELIRKAREEVAGL
                     EKGWREALMQEQDIFLEELRRHVAREVCVIARRIVKDLASAELETQVVNTFVMRIRDL
                     DKEERNALPSGTGEMNGRLAIVSAFDIGKDDRRKISEALRNTLKKSVDLEFRTSSDMV
                     LGIELRSRDHKLAWTVDDYLGSLEAGIRDVFEEEAREGIRDDEEGPQKT"
     gene            complement(1023..1292)
                     /locus_tag="PKJ17_07020"
     CDS             complement(1023..1292)
                     /locus_tag="PKJ17_07020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOD75760.1"
                     /translation="MDSITLIGMASVITSGITIAIGSVGPALGEGRAIAQALSAIAQQ
                     PDESGTITRTLFVGLAMIESTAIYCFVISMILIFANPFWDHVIAK"
     gene            complement(1274..2110)
                     /locus_tag="PKJ17_07025"
     CDS             complement(1274..2110)
                     /locus_tag="PKJ17_07025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOD75761.1"
                     /translation="MSLKEISPDAVILWKWGFVHVNLTMVFTWAIMALLVLACWIVTR
                     DLSTGTAVSRWQNLLEILVSAMRDHIRDVTRSEPGPYLSFIGTLFLFIAVSNFLAVVP
                     GYVPPTGSLSTTAALAICVFVAVPLYGIMDQGVSGYLRHYIRPTVFMLPFHVIGELSR
                     TLALAVRLFGNIMSGTKIVAILLAVTPFIFPIVIQALGLLTGMIQAYIFSILAMVYIA
                     SATRARGDGGTKRTKETARTAERTGKNNPHGPSSTGSGLAHQGMEGREGQERRRSWTA
                     SL"
     gene            complement(2147..2443)
                     /locus_tag="PKJ17_07030"
     CDS             complement(2147..2443)
                     /locus_tag="PKJ17_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOD75762.1"
                     /translation="MTEDKEQITMDIAVYLICLVSGAVLGFLHFGSLWVMVSRLGRVR
                     RPVVLVFGRFLVMMTVTLVGLYLVMGGRWERLIAALAGFMLVKVIVARRVRGGR"
     gene            complement(2436..>2657)
                     /locus_tag="PKJ17_07035"
     CDS             complement(2436..>2657)
                     /locus_tag="PKJ17_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOD75763.1"
                     /translation="RERQDRGIWFGLGMFGLVGWSVAVPTLVGLAVGVWIDMRWPGRI
                     SWTLTGLIAGVALGCLNAWYWVKRESGHD"
BASE COUNT          578 a          870 c          674 g          535 t
ORIGIN      
        1 accctgaatg ttgccggaga aaagaaggag ttctcccgat ctcaccgtgg gaagtccttt
       61 gacgcgcgcg atccccttcg agacaaatgt aaggacaccg atctcctcca ccgtcaagcg
      121 aggacgcaat tcatccatcg cccggtcgaa cgcggaaaac gtcctttcca ggacaggcag
      181 gaccgtttcc ttttgtgaaa cgctctcggt catgtctttt gcggtccctc ctcgtcatcc
      241 cttatgccct cgcgcgcctc ttcctcgaag acgtccctga ttccggcctc cagcgagccc
      301 aggtaatcat cgacggtcca ggcaagcttg tggtcccggc ttctcaattc gatcccgagg
      361 accatatccg acgatgtccg gaattcaaga tcgacgctct ttttaagcgt attcctcaga
      421 gcttccgaga tcttgcgccg gtcatccttc ccgatatcaa aggcgctcac gatggcaaga
      481 cgaccgttca tctcccctgt gccggacgga agcgcgttcc gctcctcctt atcgagatcc
      541 ctgatgcgca tgacaaaggt attcacgacc tgggtctcga gctcggcgct ggcgaggtct
      601 ttcacgatcc gccgggctat gacgcagacc tcccgggcca catgccgccg caattcctcg
      661 aggaatatat cctgttcctg catcagggct tcgcgccatc ccttttcaag cccggccacc
      721 tcttcgcggg ccttccggat cagctctttt ctctttccgt cagcctcttc ttcaacccgg
      781 gccagcagct ccccgcgctt ctcctcgacc tcgcggctct tctcctgata ggagcgggct
      841 tcctcctccg cccgtctttc cttttcctct gcgtcggtga ggcgggagac gatcttttcc
      901 tcccgtttgt ccatcgcatt tatgaccctg ccgtaaagga aatatttcag caaggccacg
      961 aggaccagga aatttacgat ctgggcaacg accgtgaacc aatcgatgag cacggaaccc
     1021 tcctactttg cgatcacatg gtcccagaag ggattggcga agataaggat catggagatg
     1081 acgaaacagt agatcgccgt cgattcgatc atggcaagac ccacgaaaag ggtgcgcgtg
     1141 atcgtccccg attcatccgg ttgctgggcg atcgcgctca gggcctgggc tatcgccctc
     1201 ccttccccga gagccggacc caccgagccg atggcgatcg tgatccccga tgtaatgact
     1261 gaagccatac cgatcagagt gatgctgtcc atgatcttct cctttcttgt ccctcgcggc
     1321 cctccatccc ctgatgcgcg agtcccgagc ccgtcgaaga ggggccatga gggttgtttt
     1381 taccggtgcg ctccgctgtt cgtgccgttt ccttcgttct ctttgtcccc ccatcgcctc
     1441 gggcccgcgt cgcggacgcg atgtacacca tcgcaagtat cgaaaagatg taggcctgga
     1501 tcattcctgt caggagaccc agggcctgga tgacgatagg aaaaatgaac ggtgtaacgg
     1561 ccagcaggat ggcgacgatc ttcgtcccgc tcatgatatt cccgaaaagc ctgacagcca
     1621 gggccaacgt cctggacagt tcaccgatca cgtgaaaagg gagcatgaac accgtcggcc
     1681 tgatatagtg cctgagatac ccggacactc cctggtccat gatcccgtac agaggcacgg
     1741 caacaaagac gcatatggcg agggccgccg tggtggaaag cgacccggtt ggaggaacat
     1801 atcccggcac cactgcaaga aaattggaga ccgcgatgaa taggaacaac gtcccgataa
     1861 aggacaagta gggccccggc tcgctcctcg tgacatcacg tatgtgatcc ctcatcgcgc
     1921 tcaccaatat ctccagaaga ttctgccacc tggaaacggc cgttcccgtc gatagatccc
     1981 gcgtgacgat ccagcaggca agcacaagca atgccatgat cgcccacgtg aagaccatgg
     2041 tcaggttcac gtggacaaac ccccatttcc agaggatgac ggcatcgggg ctgatctctt
     2101 tcaagctcat gggatccaag gaaacctcac tttgcccggg gtgcccctag cgcccccccc
     2161 gcacacgcct ggcgacaata accttgacga gcatgaaacc ggcaagagcg gcaatgagcc
     2221 tttcccatcg accccccatg acaagataga gaccgaccag cgtcaccgtc atcatgacga
     2281 ggaaacggcc gaagaccagc acaaccggtc gcctgaccct gccaagacgg gacaccatga
     2341 cccagagcga tccgaaatgg aggaatccca ggaccgctcc agataccagg cagattaagt
     2401 agacggcaat atccatggtg atctgctcct tatcctcagt catgtccgct ctcgcgtttc
     2461 acccagtacc aggcattgag acagcccagc gccactcctg cgatgagtcc cgtaagcgtc
     2521 cacgagatgc gaccgggcca ccgcatatcg atccacacgc ccacggccag accgaccagt
     2581 gtcggcacgg ctacggacca tccgacaaga ccgaacatcc ccagaccgaa ccagatgccc
     2641 ctgtcctgtc tctccct
//
LOCUS       DAOPOA010000304         2655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41931_length_2655_cov_2.974615, whole genome shotgun sequence.
ACCESSION   DAOPOA010000304 DAOPOA010000000
VERSION     DAOPOA010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41931_length_2655_cov_2.974615"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..325
                     /locus_tag="PKJ17_07040"
                     /pseudo
     CDS             101..325
                     /locus_tag="PKJ17_07040"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(449..712)
                     /locus_tag="PKJ17_07045"
     CDS             complement(449..712)
                     /locus_tag="PKJ17_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75764.1"
                     /translation="MLYTRKIIEKIWDAQGYGNLAVWADGTTAVVAPGENPVRGGKPP
                     LAIFKPIPLVARFPMLDLATHDADLLQHVETTIQEAGGEIERD"
     gene            913..2082
                     /locus_tag="PKJ17_07050"
                     /pseudo
     CDS             913..2082
                     /locus_tag="PKJ17_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
     gene            2176..2406
                     /locus_tag="PKJ17_07055"
     CDS             2176..2406
                     /locus_tag="PKJ17_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOD75765.1"
                     /translation="MPPDASAVGQQVVNLGLPEGALIILMQRESERFVPCGSTVIEAG
                     DTLLLLTTDDLVESVRARFLETGEGKDAARSG"
BASE COUNT          459 a          856 c          802 g          538 t
ORIGIN      
        1 ggaagttcag ccagctcgtg ctgatgtgcc tcaagcccgg tgaggagatc ggcgaggagg
       61 tccacgacga catcgaccag ttcttcaggt tcgaggaagg ggaagggctg tcgtgatcga
      121 cggcgtgaag cacgccgtca aggacgggag cgctgtggtt gtgccgagcg gcgcaaacca
      181 caacgtcctg aacacctcaa cgacagaaaa cctcaaactc tacacgatct actccccgcc
      241 cgaacaccgg gatggggtcg tgcgaaagac ccgcgaagag gctatggccc aggaagagca
      301 tttcgacggg aagacgaccg agtaatcgct ccctcgagtt gttccagaat catcttctct
      361 tttttgggtt ctctggcgag tccgggggcc cggacgcata tggctggtag agatcgctgc
      421 tggcctgata aggagagaga agacccctct agtcccgctc gatctcgccg cccgcctcct
      481 ggatcgtcgt ctcaacatgc tggagcaggt cggcatcgtg ggtggcaagg tccagcatcg
      541 gaaaccgtgc cacgagcggg atgggcttga agatcgcaag cggcggcttc ccgcctcgta
      601 ccgggttctc ccccggggcg acgaccgcag tcgtcccatc cgcccagacg gcgaggttcc
      661 cgtagccctg ggcatcccag atcttctcga tgatcttgcg cgtgtaaagc attcatctcc
      721 tccaggagag aacctacccc agggggcatc aaccttccgg catgatggga gcgccacaaa
      781 aatcaggagt tgcccaatcc cccgggcatc agtcgtgcac cgaccatccg gcgggtaacc
      841 acagcccacc ttcccggccc agaccgccag aggggcagcg ttaagagact ccaggcacag
      901 atcgcatcca ggatggtgct cacccttgaa ctcgtcttcc tcggcatagc gttgctcttc
      961 ctgatcagca tcattgcaaa caagttctca gagcggctcg gcgtcccggc gctcctcatc
     1021 ttcctcatcg tcgggatgct cgccggttcc gaaggcccgg gcgggattcc gttcgatgaa
     1081 ccagcagtcg cccagataat cgggatcatc gcgcttgcct acatcctctt cgccggcggg
     1141 ttcgacaccc ggtgggacga ggtccggccc atcttctggc cggggctcgc cctctcaacg
     1201 gtcggggtcc tcctgaccgc ggtctgtctc ggcgcatttg cctgcctggt cctcggcctc
     1261 tccccgctta cgggcttcct cctcggggcg gtagtctcat ccaccgacgc ggcagcagag
     1321 tcttctcggt cctccgtatg gcacgagccc gcctgaaagg caaccttgcg gccgttcctt
     1381 gagttcgagt cagggagcaa cgaccccatg gcggtcttcc tcaccaccgg cgtcatcgcc
     1441 ctcatcctca cccccggcgc atcggtgctc gcgctcgtcc cgatgttcgt gcagcagatg
     1501 gtcgtcggcg ggctcctcgg ctacgggacg ggcaaggcca tcgtcttcct cgtcaaccgc
     1561 ctcaaactcg agtcagaggg gctctatccg gtgctcaccc ttgccatggt gctgatgacc
     1621 tacgggctga ccgccaccct gggcggcaac gggttcatcg ccgtctacgt cagcgggctt
     1681 gtggtgggca acagcatcgt cgtctacaaa aagagcctga tccggttcca tgacgggatc
     1741 gcgtggttga tgcagatcgt gatgttcctc gcactcggcc tcctcgtctc cccctcagac
     1801 ctcgtgccgg tgatcgtccc cgggcttctc atcgccttct tcctgctgct ggtcgcccgg
     1861 ccggttgccg tcttcatcac gcttcttccc tggaagatgc cgctgaacga gaaggccctg
     1921 gtctcctggg tggggctccg gggggccgtc ccgatcatcc tcgcgaccta cccgcttgtt
     1981 gccggggtgc cggacgcgga gatgatcttc aatatcgtct tcttcatcgt cctggtctcg
     2041 gcacttgtcc acgggacatc catccccacg gtcgcccggt gactcggcct tgccgctccg
     2101 ctccaggaga agcgcaaact ctcccgggag tttgaggtcg accccaacgc cccgagcgaa
     2161 ctcctcgaac tgaccatacc ccctgacgcc tcggcggttg ggcagcaggt cgtcaacctc
     2221 gggttgccgg aaggggcgct catcatcctg atgcagaggg agagtgagcg gttcgtcccg
     2281 tgcggcagca ccgtcatcga ggccggcgat acgctcctcc tcctgacgac cgatgacctg
     2341 gtagagagtg tcagggcccg gttcctcgag accggtgaag ggaaggatgc ggctcgctct
     2401 ggttagcggg agatgagcag gttttgccgg aacccagggg gggacatggt caggaggtgg
     2461 atgttttgga tgatgccggt gtctcgctgg attgaaagcc cggtaacgag cccctccatg
     2521 ttgtatggac gaaggcgaca tttaggatag actcgacctc tctctccgcc cccaggacca
     2581 cctgcatgag gcggggtttt gtgttctaga attttgatga ctttctgagg tgtgagtgcc
     2641 ggaaggttag gcatg
//
LOCUS       DAOPOA010000305         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41963_length_2653_cov_3.483064, whole genome shotgun sequence.
ACCESSION   DAOPOA010000305 DAOPOA010000000
VERSION     DAOPOA010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41963_length_2653_cov_3.483064"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..>879)
                     /locus_tag="PKJ17_07060"
     CDS             complement(327..>879)
                     /locus_tag="PKJ17_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75766.1"
                     /translation="TTKETGKGTGLGLATVYGIVKQHNGYITVYSEPDHGTAFHVYLR
                     AAMTKVETGIDTTVPAGRGKETVLIAEDDEEVRHIMREVLEEYGYTIVEAVDGEDAID
                     KFELHSSIDLVILDSVMPRKNGRETFERIRAMDSHIKVLFISGYTRDVVLDKGIKDRE
                     FDFIAKPLILDRFLQKIREILDR"
     assembly_gap    880..889
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<890..2640)
                     /locus_tag="PKJ17_07065"
     CDS             complement(<890..2640)
                     /locus_tag="PKJ17_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD75767.1"
                     /translation="MRYVNARCAEIFGYTVDEAIDRLSVRDVIFSGDLPLVEASIGKR
                     TSGELPSHHYEFRIVTKTGDIRYVEVYSSYTVHEGKSAVIGTILDISERKRAENALMA
                     SEERFRTFFNLPIVGFAITGADSRWIHFNDKLCEMLGYSREELERTTWMRITPSEDLA
                     RERPLFEDVVRGGRNTAGIEKRYVTKDGSLADVFVSTQVVRNPDGSVAYFASIIQDIT
                     AQKRAEGEIIREREKLRTLSDNAPFGMVLIDEDGRFTYVNAKFTELFGYGLSDIPDGR
                     TWFRKAYPDAEYRHTVISTWLEDFEDTGPAERKPRVFTVTCNNGIKKMIEFIPSKLAS
                     GDYLMTCEDITELVRLESQLRQAQKMESIGTLAGGVAHDFNNILTTLIGYASLIQTKM
                     DRDNPLRSYVDQVLSASRKGADLTRSLLTFSRQQPVTLLPVDLNDAIRATEPLLKRLL
                     TEDIELTTSLTGEDTVVMADRSQMDQILFNLVTNARDAMPGGGRLTVETDIADMDDWF
                     IEAHGFGEPGRYVLMTVSDTGEGMDEATREKIFDPFFTTKEKGTGLGLAVASKIVEQH
                     GGELLSENQPGRGLTMTV"
BASE COUNT          493 a          771 c          728 g          651 t
ORIGIN      
        1 cgtgacggtc ttttccgggt gaatggttgt ggctccgggg tcgatcggga tcgaatcgag
       61 cgggccggcg tataccgatg tcaccggcat tatggccagc accgcaatac ctgagatgaa
      121 cagcgctttt gcacataccc ttaataatct tctcaaggtt cgcctcgtaa gttggaagtt
      181 taatacccct gcaaaagtgc ttctaaccca gaaatagatt agaaaaagag gtcttgtatg
      241 tcaagccaat tcatattgaa tgaaagatct cggattcccg gaatgccggg tacacatgcc
      301 gggacgggtc ttcgcgggat agcctgctat ctgtccagta tctcgcggat cttctgcagg
      361 aacctgtcga gtatgagcgg tttggcgatg aaatcgaact ccctgtcctt gatccctttg
      421 tcaaggacga catccctggt atatccgctg atgaagagca ccttgatgtg ggagtccatc
      481 gcgcgtatcc tctcgaaggt ctcccttccg ttctttctgg gcatgaccga gtcgaggatg
      541 acgaggtcta tgctgctgtg cagttcgaac ttgtctatcg cgtcttcgcc gtccaccgcc
      601 tccacgatcg tgtaaccata ctcctctagc acttccctca tgatatgacg cacttcttcg
      661 tcgtcctccg cgatgaggac cgtctctttc cctcttcccg cgggtaccgt cgtatcgatc
      721 ccggtttcga cctttgtcat cgctgcccgg agatagacgt ggaaggccgt gccatgatca
      781 ggttcgctgt acaccgtgat atagccgttg tgctgcttca cgatgccgta aacggtcgcg
      841 aggccgaggc ccgtgccctt gcccgtctcc ttggtggtgn nnnnnnnnna cggtcatggt
      901 gaggccccgg cctggctggt tttcggacag cagttccccg ccgtgctgct cgacgatttt
      961 ggaggccacg gccaggccca gcccggtgcc cttctccttg gtggtgaaga agggatcgaa
     1021 gatcttctcc cgcgtcgcct catccatgcc ttcccccgtg tcggaaacag tcatcaacac
     1081 ataccggccg ggctccccga acccgtgggc ctcgatgaac cagtcgtcca tgtccgcgat
     1141 gtccgtttcc acggtgagcc tcccgccccc gggcatggcg tccctggcgt tcgtgacgag
     1201 gttaaagaga atctggtcca tctgcgacct gtcggccatg accaccgtgt cctcgccggt
     1261 gagcgacgtg gtgagttcga tgtcctcggt gaggagtctc ttcaacaatg gttccgttgc
     1321 tcgtatcgcg tcgttgaggt ccaccgggag gagagtgacg ggttgctgtc tgctgaaggt
     1381 cagaaggctc cgcgtcaggt ctgcaccctt cctcgaggcc gagaggacct ggtcaacgta
     1441 tgagcgcaag gggttgtccc tgtccatctt tgtttgtatc agggaggcat accctatgag
     1501 ggtggtgagg atgttgttga aatcatgcgc gacacctccc gcaagggtcc cgatggactc
     1561 catcttctgg gcctggcgca attgcgattc cagccgtacc agttcggtga tatcttcgca
     1621 ggtcattaga taatccccgg aagcaagttt cgaaggtatg aactcgatca tcttctttat
     1681 cccgttgttg catgtaacgg tgaacaccct cggtttccgt tcggcgggtc cggtgtcctc
     1741 gaagtcctca agccaggtcg agatgaccgt atgccggtat tcggcatcag ggtaggcctt
     1801 tctgaaccag gtcctgccgt ccgggatatc cgacagcccg tacccgaaga gctccgtgaa
     1861 cttcgcgttg acgtatgtga aacggccatc ctcgtcgatg aggaccattc caaagggggc
     1921 attgtccgaa agggtcctca gtttttccct ttccctgatg atctctccct cagccctttt
     1981 ctgggccgtt atgtcctgta tgatggaggc aaaataggcc accgaaccat cggggttacg
     2041 gaccacctgg gtggagacga agacatcggc aagggaaccg tccttcgtga catatcgttt
     2101 ttcgataccg gcggtgttcc ttccgcccct taccacatct tcgaagagag gtctttccct
     2161 ggcgaggtcc tcggatggtg tgatccgcat ccaggtcgtt ctttccagtt cctctcggga
     2221 atacccaagc atttcgcaaa gtttatcgtt gaaatggatc cacctgctgt cggccccggt
     2281 tatcgcgaaa cccacgatgg gaaggttgaa gaaagtgcgg aaccgttcct cgctcgccat
     2341 cagggcgttc tcggcacgct tgcgctcgga aatatccaga attgtcccga taacggccga
     2401 ttttccttca tgcacggtgt aggagctata gacctcgacg tacctgatgt ccccggtctt
     2461 cgtgactatc ctgaactcgt aatggtgtga cggcagttct cccgaggtcc tcttgccgat
     2521 gctggcctct acaaggggga ggtccccgga gaatatcacg tccctcacgc tcagccggtc
     2581 gatcgcctcg tcgacggtgt agccgaatat ttcggcacac cgcgcgttga cgtaccgcac
     2641 caccccatcc ggc
//
LOCUS       DAOPOA010000306         2652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_41987_length_2652_cov_3.205622, whole genome shotgun sequence.
ACCESSION   DAOPOA010000306 DAOPOA010000000
VERSION     DAOPOA010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41987_length_2652_cov_3.205622"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..977)
                     /locus_tag="PKJ17_07070"
     CDS             complement(360..977)
                     /locus_tag="PKJ17_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOD75768.1"
                     /translation="MIKLFVFDLGNVILPFEHRQIAVKLHEASLIKDRFTPDDIFRYL
                     FDYEKGFVNAYEEGRLSSKDFYKKIKERYKLELELEEFKDIWNDIFEEDPAVSEIILY
                     LKERGYPIVLLSNTNELHFSYVMERFPIIHHFDEWILSFEVGAKKPQEKIYNMVFETR
                     SLERHEALYIDDIPEYIAAAAGYGIPGIVFKEAADVWKVIRENCV"
     gene            complement(970..1638)
                     /locus_tag="PKJ17_07075"
     CDS             complement(970..1638)
                     /locus_tag="PKJ17_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75769.1"
                     /translation="MARPDNEVSPCAFYMLGIIHRDRRNDELLGKWIDALTPEVITLE
                     LSPYGLAFRQAMGKAYRRRIDDIVRSLEGEGHPCLSGEVEDLYAYVDVPREFTIADEY
                     CRRSGARLYPVDMDLFSWMKLREIDELINRTNVEKSVAFRVREGRGAENVLASLYFRS
                     GVSAFSYTDEMALRDGFMCRGIGALTKRFSGKRFLHIGGWRHLTDPLGFYARFQPVKI
                     FPYD"
     gene            1775..>2652
                     /locus_tag="PKJ17_07080"
     CDS             1775..>2652
                     /locus_tag="PKJ17_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOD75770.1"
                     /translation="MKTMLCVFCLLFAGQALATDMIIGGDVTHTVGKGDNLYRVGARY
                     GVFWKNIARDNGLDEKQPLVEGTVLRLNTRKIVPRMIDNGIIINIPDRTLYFFQDKQL
                     MALPVGLGAIFQNEYSDWKTPEGKFRITEKRKDPTWYVPESIQIEYALKGKPVEESVP
                     PGPKNPLGKYAIKTSLPAVLIHETIWPGSVYRYMSHGCVRMLREHIEQLFPLVQVNMQ
                     GEIIYEPVKIAQTRDGRTYVEIRTDVYRKHRSIRGHLSKLLEARGIADKVDWQKIDKL
                     IKEESGVAEDVSLQPP"
BASE COUNT          654 a          741 c          686 g          571 t
ORIGIN      
        1 atcccgggga atctgaggtt tcctgccccc ccggagctgg tacacggtaa gcccgtatcc
       61 ggccagaaac ccgaggatgt acatgagccc ataccagcgc atggcgaaag ggccgattct
      121 aaaaatgacg gggtctatct cgggaaaagg aagcatggct agttgtggcc tccgtggttc
      181 tctaaaacaa aatgaccaat gaccaagcac caataaccag acaagaagac aacaaccaat
      241 aaccaaattt caacggaacg gctctcattc tcactgttgt tctcgttcgg tcatcttgtc
      301 cttgaatcac tgcagtctgt ttggttattg tgatttggtc attgtttatt gtcttctttt
      361 catacacaat tctctcgtat caccttccag acatcggcgg cctccttgaa aactatcccg
      421 gggatgccgt atcccgcggc cgccgctata tactccggga tgtcgtcgat atagagcgct
      481 tcgtggcgct ccagcgagcg cgtttcgaat accatgttgt agatcttttc ctggggtttc
      541 tttgcgccca cctcgaagga gagtatccat tcatcgaaat ggtgtattat gggaaacctt
      601 tccataacat aggagaagtg aagctcgttc gtattgctta agaggacgat gggatacccc
      661 ctctccttga gatagaggat gatctcgctg accgccgggt cttcctcgaa tatgtcattc
      721 cagatatcct tgaactcctc gagctcgagt tcaagcttgt acctctcctt gatctttttg
      781 taaaaatcct tcgaagagag gcgaccttcc tcgtaggcgt tcacaaaacc cttctcgtag
      841 tcgaagagat accggaagat atcatcaggg gtaaagcggt ccttgatgag cgaggcctcg
      901 tggagcttta cggctatctg cctgtgttcg aagggaagga tcacgttgcc caggtcgaag
      961 acaaagagct taatcatagg ggaatatctt cacgggctgg aatcgggcgt agaaaccaag
     1021 ggggtccgtg aggtgccgcc atccgcctat atgcaggaaa cgctttccgc tgaaacgctt
     1081 tgtaagcgcc ccgatgccgc ggcacatgaa cccgtcgcgc agcgccatct cgtcggtgta
     1141 tgaaaaagcg ctcactcccg accggaaata aaggcttgcc aggacattct ccgccccccg
     1201 accttcccgg accctgaatg ccacgctttt ctccacgttt gtccgattga tgagctcgtc
     1261 tatctcccga agcttcatcc aggagaaaag gtccatgtcg acaggataca gacgcgcgcc
     1321 gctccgcctg caatactcat cggcaatggt gaactcccga gggacgtcga cgtaggcata
     1381 aaggtcttcc acctcgccgg acaaacaggg atgcccttcg ccctccaggg accgaacaat
     1441 atcgtcgatc ctcctccggt aggcctttcc catcgcctgc cggaaggcaa gaccgtacgg
     1501 ggacaactcc aacgtaatga cctcgggcgt cagggcatcg atccattttc caagaagctc
     1561 atcgtttcgc ctgtcccggt ggatgatccc cagcatgtag aaggcacaag gagagacttc
     1621 gttatcaggg cgcgccatga ctaaatttac gcacgacccc ggggacgttt caagagaaat
     1681 gtacaaaccg ccgcgttatc attctcactt tttctgttgt caatttcccc gaggtatact
     1741 agaataacct cctgtcatac ctatgaaaac atcaatgaaa acgatgctct gtgtattctg
     1801 tctactcttt gccggccagg cgctggcaac agacatgatc atcggcggcg atgtgacgca
     1861 cacggtgggc aaaggcgaca acctttaccg cgtgggcgcc aggtatgggg tcttctggaa
     1921 gaacatcgcc cgggacaacg gactcgacga aaagcaaccc cttgtggagg gcaccgtcct
     1981 gagacttaac acgcgcaaga tagtcccgcg tatgatagac aacggcatca tcatcaatat
     2041 acccgacagg accctctact tctttcagga caaacaactg atggccctgc ccgtcgggct
     2101 cggagccatc tttcagaatg agtacagcga ctggaagacc ccggagggca agttccggat
     2161 aacggagaaa aggaaagatc cgacctggta cgtgccggag tcgatccaga tagaatacgc
     2221 gttgaaaggc aaacccgttg aggagtccgt cccccccggc ccgaagaacc cgctcgggaa
     2281 atacgccatc aagacatcac tgccggccgt cctcattcat gagaccattt ggccggggag
     2341 cgtctacagg tacatgagcc atgggtgcgt ccggatgctc agagaacaca tagagcagct
     2401 tttccccctc gtgcaggtga acatgcaggg ggagatcatc tacgaaccgg tgaagatcgc
     2461 gcaaacacgg gacggcagga cctacgtcga gatccgcacc gacgtctaca ggaagcatcg
     2521 ttccataaga gggcatctct cgaagctcct cgaagcccgc ggcatcgcgg acaaggtgga
     2581 ctggcagaaa atagacaaac tcatcaagga ggagtcgggg gtggctgagg acgtgagcct
     2641 tcaaccgccg aa
//
LOCUS       DAOPOA010000307         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_42037_length_2650_cov_2.873218, whole genome shotgun sequence.
ACCESSION   DAOPOA010000307 DAOPOA010000000
VERSION     DAOPOA010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42037_length_2650_cov_2.873218"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PKJ17_07085"
     CDS             complement(<1..630)
                     /locus_tag="PKJ17_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOD75771.1"
                     /translation="MSKEFNIGVIPGDGTGPEVVAEGVKVLTAASKKFGFSLKLTYYD
                     IGGERYKRTGETLPDSVLSELKQFDALYLGAIGHPEVRPGILEKEILLRTRFELDQYI
                     NLRPVKLYEGVETPLKDKGPKEIDFVVVRENTEGLYTGSGGVLKKGTKDEVAVQESIN
                     TRKGVERCLRFAFEYARRRNREKKLTLCGKTNVLTFAFNLWERAFYEVAK"
     gene            complement(655..1941)
                     /gene="purD"
                     /locus_tag="PKJ17_07090"
     CDS             complement(655..1941)
                     /gene="purD"
                     /locus_tag="PKJ17_07090"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOD75772.1"
                     /translation="MNVLVIGSGGREHALCWKLSTSTGVDKLYCVPGNGGISDIAECH
                     DINVGDTAGLLEFTKRMKVDLVVVGPENPLAGGIVDTFEAKGIPIFGPVARGAAIEAS
                     KIFSKELMRDSSVPTAPFQVFDDHKNALGYIRAKEPPFVIKADGLCAGKGAYVIKDTA
                     EAESVLTELMVNGIYGEAGKKVVIEEFLPGVEASYLAFADGNTILPMPASQDHKPLLD
                     NDLGPNTGGMGAYTPIPFLNDGLEREIREGIMLKTIEAMREKGISYKGVLYGGLMLSG
                     GRPYVIEFNARFGDPETQPIIFKMESDLLPVLVACVEGRLRDVGEIRWKEGVSVCVVL
                     ASKGYPEKPEKGKVIRGLDRLAGKEDIMVFHAGTKKAGSEYVTSGGRVLGVTALGSTY
                     EDAVNKAYEAVNLIDFEGMHYRRDIGKKALTVGRTL"
     gene            complement(1960..2544)
                     /locus_tag="PKJ17_07095"
     CDS             complement(1960..2544)
                     /locus_tag="PKJ17_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75773.1"
                     /translation="MKIRRAVVSVSSKEGLSDLALFLNKLGVEILSTGGTRKYLEGIG
                     LGPIEVSTYTGFPEIMDGRVKTLHPKVHGGILNIRDNEEHQQAMRTHGISHIDMIVVN
                     LYPFIEVVSKGCTFEDAIENIDIGGPSMIRAAAKNFKYVTVVVDPEDYSKVMENMAQN
                     DGATTEELRFYLAKKVFALTSAYDKAITDYLSKI"
BASE COUNT          515 a          848 c          664 g          623 t
ORIGIN      
        1 cttcgccacc tcgtagaagg cgcgttccca gagattgaag gcgaaggtga gcacgttcgt
       61 cttgccgcaa agggtcaact ttttctcccg gttccttctc cgggcgtatt cgaaagcgaa
      121 cctcaggcac cgctcgacgc ccttgcgagt attgatcgac tcctgtacgg ccacctcatc
      181 cttcgttccc ttcttcagca cgccaccgga accggtgtaa agcccctccg tgttctccct
      241 gacgacaacg aaatctatct ctttcggccc cttgtccttc agcggcgtct ccacgccctc
      301 atagagcttt accggtctca ggttgatata ctggtcgagt tcgaagcggg tcctcaggag
      361 gatctccttc tccaggatac ccggtctcac ttcgggatgg ccgatagccc ccagatagag
      421 tgcgtcgaac tgcttcagtt cgctcaagac actgtcaggc agcgtctccc cggtcctctt
      481 gtagcgttcc ccgcctatgt cgtaatatgt gagtttcagc gaaaaaccga acttcttcga
      541 tgcggccgtg agaaccttga caccctcggc cacaacctca gggcctgtcc cgtcgccggg
      601 tatcacaccg atattgaatt ccttcgacat tgaaacctcc tgcctttctt gtctttatag
      661 cgtccgcccc accgtcaggg cttttttccc gatatccctg cgatagtgca tcccctcaaa
      721 atcaatgaga ttcacggcct cgtaggcctt attgacagcg tcttcatacg ttgaccccag
      781 tgccgtcaca ccgaggaccc ggccaccgga ggtcacgtat tccgaacccg ccttcttcgt
      841 gccggcgtgg aataccatga tgtcctcctt ccccgccagc ctgtcgaggc cccggatcac
      901 cttgcctttc tccggcttct ccgggtatcc ctttgaggcg aggacgacgc acacgctcac
      961 tccttctttc caccgtatct ctcccacatc cctgagtctg ccctccacac aggcgacgag
     1021 gacggggagc agatcgctct ccatcttgaa gattatcggc tgtgtctccg gatcgccaaa
     1081 acgcgcattg aattcaataa cgtagggtct tccgcccgag agcatcaatc ccccgtagag
     1141 aacccccttg taggagatgc ctttctcgcg catggcctct atcgttttga gcatgatccc
     1201 ctcacgtatc tcacgctcca gaccgtcgtt gaggaagggt atcggcgtgt acgcgcccat
     1261 cccccccgta ttcggcccga ggtcgttgtc gagaagaggc ttgtggtcct gcgaggcagg
     1321 catgggaagg atcgtgttgc cgtctgcaaa ggccagatag gacgcctcca caccgggcag
     1381 gaattcctcg atgaccacct tcttgccggc ctcgccatag ataccgttga ccatgagctc
     1441 tgtgaggacc gattccgcct ccgccgtatc cttgatgacg taagcgccct tccccgcaca
     1501 gaggccgtcc gccttgatga cgaagggggg ctccttcgcc cgtatgtatc ccagggcgtt
     1561 cttgtggtcg tcgaagacct ggaagggcgc cgtgggcacc gaactgtccc tcatgagctc
     1621 cttcgagaag atcttgctcg cttcgatggc ggcgccccgg gcaacaggac cgaatatggg
     1681 gatacccttc gcctcgaagg tgtcgacgat cccccccgca agggggttct ccggaccgac
     1741 gacaacgagg tcgaccttca tcctcttcgt gaattcgaga agcccggccg tatctcccac
     1801 attaatgtca tggcattcgg ctatgtccga gatgccgccg ttgccgggaa cgcagtacag
     1861 cttgtcgacg cccgtcgacg tggagagctt ccagcacagg gcgtgctctc tccctcctga
     1921 tcctattaca agaacgttca caccaacccc cgggttcagt tagattttcg acaagtaatc
     1981 ggtgatggcc ttatcatagg cgcttgtcag agcgaagacc tttttcgcca ggtagaacct
     2041 caattcctcc gtcgtggcgc cgtcattctg tgccatgttc tccattacct tcgaatagtc
     2101 ctcgggatcg acgacgacgg tgacatactt gaagttcttg gcggcggcac ggatcatcga
     2161 ggggcccccg atgtctatgt tctcgatggc gtcctcgaag gtgcacccct tgctcaccac
     2221 ctcgataaaa ggatagagat tgacgacgat catgtcgata tgactgatcc cgtgcgttct
     2281 cattgcctgc tggtgctcct cgttgtcacg gatgttcagg atcccgccgt ggaccttggg
     2341 atgcagcgtc ttcacccggc cgtccatgat ctcgggaaaa cccgtgtagg tactgacctc
     2401 gatgggacca agtccgatcc cttccagata ctttctggtg ccccccgtcg acagtatctc
     2461 gacgccgagc ttgtttaaga ataacgccag gtcagacaaa ccctccttgc tggaaacgct
     2521 gaccacggca cgcctgatct tcataacaac ctccttcgcg cgatataatc aagaatatac
     2581 gggtaaagac aagggaaaac tgcgtgtctg ccgctctcta agctgtaata gtaaatgctg
     2641 gctcactcct
//
LOCUS       DAOPOA010000308         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_42124_length_2645_cov_3.193822, whole genome shotgun sequence.
ACCESSION   DAOPOA010000308 DAOPOA010000000
VERSION     DAOPOA010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42124_length_2645_cov_3.193822"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..722)
                     /locus_tag="PKJ17_07100"
     CDS             complement(36..722)
                     /locus_tag="PKJ17_07100"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOD75774.1"
                     /translation="MKTLPSILVVLLAATAIVCFGGDVFAQKTQPAGAKAIFDSGEGP
                     AVRMSSGPAVKTPTQTAAAPAREKYVGISYKLVLLRDDGRFDIVPRSRTFRSGERLKL
                     LVRTNKPGYLTIMNIGTSGNTTVLCSEQVEAGTMMEIPRGGNFRFSGAPGTEKLLVML
                     SGQPNPMGMPSQGQTMAQAPAAPPPPGGTDAALPPPPPPGQADVANIPPPPPPGVMTA
                     SLTGGKDITL"
     gene            1158..>2645
                     /locus_tag="PKJ17_07105"
     CDS             1158..>2645
                     /locus_tag="PKJ17_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75775.1"
                     /translation="MRIPKTLKINLLLLVCVFLLVPMFLSAVTMPPSQIVNEAMDGVL
                     VDMGNPFLKVIKQGSWISGENYRNPLTNLLDPSDHDARLFISLDDVGPERAREVWKAY
                     QGRLRKKISAMAKSAGYSTDEIQLLLKSVNFYPPAQLLKGIEQNEQAIAKFLKMGNYP
                     NLLEVGEEAAEGLYTTPTKFIRQGYETGKRGQVVELVADEGSDSYRLVRKATAEAEHV
                     AETVGEANLKGYVQSAEYSLNEAQKALKEGKLDTAKKNLSRAKDRLKSARKLGGVSAE
                     GSAVKELDALEGQIDDAIKLAFESADDAKAMLAKTKQFANRLGGEVMKKTNVELAALE
                     SMAVKNSVKQRAFLKGVLEGEGKWATLRVNLEEASGSALTTLKSGARLAYRNWFMSLI
                     ALWEMKEFPAAIEREGAAKASARLAVTIAGLANPTFGYAQLFSMIFMTTGELLVDWIS
                     SYGYGAVVGRQDCMDLIAGMYTVPGREQNIEEKGCEQIQDDRQLAC"
BASE COUNT          568 a          746 c          848 g          483 t
ORIGIN      
        1 ggcgagataa cggcatagct cgtcttgttc ccgtcctaca gggtgatgtc cttgccgccc
       61 gtgagggatg ccgtcatgac acccggggga ggaggagggg ggatgttcgc gacgtcagcc
      121 tgtcccggag gaggcggagg ggggagggcc gcatcggtgc cgcccggagg gggcggagcc
      181 gccggtgcct gtgccattgt ctgcccctgt gagggcatgc ccatggggtt cggctggccg
      241 gagagcatga caaggagctt ttccgtgccc ggagcgccgg agaacctgaa attgccgcct
      301 ctgggtattt ccatcatcgt gccggcctct acctgctcgc tgcacaggac cgtcgtgttc
      361 cccgaagtgc ctatgttcat gatcgtgagg tagccgggtt tgttggtcct gacgagaagt
      421 ttcaggcgtt cgccggagcg gaatgtcctc gaccggggaa cgatgtcgaa cctgccgtca
      481 tcccggagga ggacaagctt gtatgagatt cctacgtact tctctctggc aggcgcagct
      541 gccgtctgcg tgggggtctt caccgccggt ccggaagaca tcctgaccgc cggcccttca
      601 ccgctgtcaa agatggcctt tgcgccggcc ggctgggtct tctgggcgaa gacatcgccg
      661 ccgaagcaca cgatggcggt ggcggcgaga agaacgacaa gaattgaagg caatgttttc
      721 atgagaacct ccctgctgtt aagaaatgtt gaattcctct tactggacta tcgtccacta
      781 tgcaattgtc ccggatcaac ggtacctggt gtgcatgcga aatatcgctg tgccgtaccc
      841 atggtgtagc acgtagtata actggaaccc cgaaattttg tcaacgaaag accttgagga
      901 ccggtgtccg ggtcccccat atggagagcg acgagagggt caaaattccg acaagtgagg
      961 ccgggccttc cttcgcgcgc aggtggtgta gtgcagcctc atattaatat tgacaatccg
     1021 cgtgcgtcgc cgatatattg cgtcaaaagg caaagggtgc cctcgcggga tatccgtcgc
     1081 tttgcccttg ggccagacaa accggccgcg gattcaccaa gcagtctaca ttccagtaag
     1141 ttccgaaggg ggatcgcatg cgcattccaa aaactctgaa gatcaatctc cttctccttg
     1201 tctgtgtgtt ccttctcgtg ccgatgttcc tctctgccgt cacgatgcct ccctcccaga
     1261 tcgtgaacga ggccatggac ggcgtgcttg tcgacatggg caatcccttt ctcaaggtca
     1321 tcaagcaggg gtcgtggata tcgggcgaga actaccgcaa tcccctcacc aatctcctcg
     1381 atccttccga ccacgacgcc aggcttttca tctcccttga cgacgtggga cccgagcggg
     1441 cgcgggaggt ttggaaggcc taccaggggc gcctgagaaa gaagatatcc gccatggcga
     1501 agagcgcggg atacagcacc gacgagatac agcttctcct taaatccgtg aacttctacc
     1561 cccccgcgca gctcctgaag gggatagagc agaacgaaca ggccatcgcc aaatttctga
     1621 agatggggaa ctaccccaac ctcctggagg tgggtgagga ggccgcggaa ggcctctaca
     1681 cgacgcccac gaagttcatc cgccagggat acgagacggg aaaaaggggc caggtcgtcg
     1741 agctcgtggc agacgagggc tcagactcat accgcctggt ccggaaggca acggccgagg
     1801 ccgagcatgt tgccgagacg gtcggcgagg ccaatctgaa gggatatgtc cagtcggcgg
     1861 agtacagcct gaacgaggcg cagaaggccc tgaaggaggg caagctcgac acggcgaaga
     1921 agaacctctc ccgcgcgaag gaccgcctga agagtgccag gaaactgggc ggagtcagcg
     1981 ccgaaggctc ggcagtcaag gagcttgacg cgctggaggg tcagatagac gacgccataa
     2041 agctcgcctt tgaatcggcg gacgacgcga aggcgatgct cgcgaagaca aagcagttcg
     2101 cgaacaggct cggcggagag gtcatgaaga agaccaacgt cgaactcgcc gccctggaat
     2161 cgatggcggt aaagaactcc gtgaaacagc gggcattcct gaaaggggtc cttgaaggcg
     2221 agggcaagtg ggccactctg agggtgaacc tcgaggaggc ctcgggcagc gctctcacaa
     2281 ccctgaagag cggggcacgg cttgcgtaca ggaactggtt catgtccctt atcgccctgt
     2341 gggagatgaa ggaattcccg gcggccatcg aaagggaggg ggcggcgaag gcgagcgcac
     2401 ggctggctgt gacgatcgcc ggcctcgcga accccacctt cggctacgcg cagctcttca
     2461 gcatgatatt catgacgacg ggggaactcc tcgtcgactg gatcagctcc tacgggtacg
     2521 gcgccgttgt gggcagacag gactgcatgg acctcatcgc cggcatgtac acggttcccg
     2581 gcagggagca gaacatcgag gagaaggggt gtgaacagat acaggacgac aggcagctcg
     2641 cctgc
//
LOCUS       DAOPOA010000309         2643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_42168_length_2643_cov_3.578439, whole genome shotgun sequence.
ACCESSION   DAOPOA010000309 DAOPOA010000000
VERSION     DAOPOA010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42168_length_2643_cov_3.578439"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PKJ17_07110"
     CDS             complement(<1..293)
                     /locus_tag="PKJ17_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOD75776.1"
                     /translation="MNEERSKKDGGGEKCYAFPNRPTPGMPVVIDETRCTGCNKCVTI
                     CTTDVLVANPEKGKPPIVMYPEECWYAACCVGECPEECLTMRHPLMMRVHFKN"
     gene            complement(314..2302)
                     /locus_tag="PKJ17_07115"
     CDS             complement(314..2302)
                     /locus_tag="PKJ17_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOD75777.1"
                     /translation="MNWEDAVTQTGQVIEWPYPVRYDRQNDIDVDVVVLGGGPGGCMA
                     AISAAKKGLKVALLDKAYPKRSGGGSGFDHWLNTPNPASKVTAEDCVEWERVTFKGYS
                     NSLSRYVAAREAYDTLLEIEKMGAKVRDIDDTFKGAPFRDEKTKFVYCYDYENRFQFR
                     VWGATFKPAIFNECLQLGVQVFDRVVATSLLTEGGKQGARVVGATAINMRTGEFYVFR
                     AKATINAMSCHQGSWRFSTESIGLDMFKPNITGDGPAIMWRAGVELAQMERSRAAMPP
                     GYEYPSYGTGNWWNTWHPATIVDANGKEVPYVDSDGNVVPVERRCVPRPGQKFIAERS
                     RDPLYAKPTMPADLADRIRRGEFVLPLYADLAAMPWYERDAIWGLMVGNEGRTKIPVL
                     VNYETAGFNSHRDMLQNYFTLSGEPYPSMDSTMALGPYRMEAFGNGGEVVVDWDLRTN
                     LEGLYAVGDAILAGNYWYHACATGRYAGRKAAEYAIQLEGPPSIDAAQVERERERVYA
                     PTKRKPGKDWVDWKELRFAGARAMQNYCGAVRNEGMMKMGLKWIEDIKNDHYPEAHAS
                     NPHILLRVLETYNMLTVDEIIFQACMARKASSEVLGHFRQDYPEMDPPEWHKWITIKQ
                     VDGATRTGDLALDFQGDLAGNYEAHNPNYRGYLKKKKA"
BASE COUNT          446 a          844 c          724 g          629 t
ORIGIN      
        1 ttgttcttga aatgcaccct catcatgagg ggatgccgca tggtgagaca ttcctcgggg
       61 cactcaccga cgcagcaggc cgcgtaccag cactcctcgg ggtacatgac gatcggcggc
      121 ttgcccttct cggggttggc cacgagcacg tcggtcgtgc atatggtaac gcacttgttg
      181 caccccgtgc atcgtgtctc gtcgatcacc acaggcatgc cgggagtcgg tctgttggga
      241 aaggcgtagc acttctcgcc gccgccatct ttcttcgatc tctcttcgtt cattctcctc
      301 gcctcctgcc tcgtcaagcc ttcttctttt tcaggtatcc cctgtagttc gggttatggg
      361 cttcatagtt cccggcgaga tctccctgaa aatcgagggc aaggtcgccg gtcctcgtcg
      421 caccgtcgac ctgcttgatc gtgatccatt tgtgccactc gggcgggtcc atctcgggat
      481 agtcctggcg gaagtgcccg aggacctcgc tgctcgcctt cctggccatg caggcctgga
      541 atatgatctc gtcgacggtc agcatattgt atgtttccag aacacgcaga agtatgtgcg
      601 ggttgcttgc gtgggcctcc gggtagtgat cgttcttgat gtcctctatc catttgaggc
      661 ccatcttcat catcccttcg ttcctgacgg ccccgcaata attctgcatg gcccgggcac
      721 cggcgaaacg cagctctttc cagtcgaccc agtctttgcc gggcttcctc ttcgtgggag
      781 cgtagaccct ctcccgttcg cgctcaacct gggccgcatc tatggagggt ggaccctcaa
      841 gctgaatcgc gtattccgcc gccttcctgc ccgcgtacct tcccgtggcg caggcgtggt
      901 accagtagtt ccccgccagt attgcgtcgc cgaccgcgta gaggccctcg aggttcgtcc
      961 tcaggtccca atcgaccacc acctctccac cgttgccgaa ggcctccatg cgatacggtc
     1021 cgagggccat ggtgctgtcc atgctgggat agggctcgcc gctcagggta aagtagttct
     1081 gaagcatgtc acgatgagag ttgaaccccg ccgtctcgta gttgacaagg actgggatct
     1141 tcgtccgtcc ctcgttcccc accatgaggc cccatatcgc atcccgttca taccagggca
     1201 ttgccgcaag gtcggcgtac aggggaagga caaattctcc tctcctgatg cggtccgcaa
     1261 ggtccgcggg catcgttggt ttggcataga gcgggtcccg ggaccgctcg gctatgaact
     1321 tctgtcccgg cctcggaaca cagcgccgct ccacgggcac gacgttgccg tcggaatcca
     1381 cgtaggggac ctccttgccg ttggcatcca cgatggttgc cggatgccac gtgttccacc
     1441 agttgcccgt gccgtaggag gggtattcgt atcccggcgg catggcggcc ctgctccttt
     1501 ccatctgcgc gagctccaca cccgccctcc acatgatcgc gggcccgtcg cccgttatat
     1561 tgggcttgaa catgtcgagg ccgatggact cggtcgagaa acgccagctc ccctggtggc
     1621 aggacatggc gtttatcgtg gccttcgccc tgaagacata gaattcgccg gtccgcatgt
     1681 tgatggccgt ggcaccgaca acacgcgcgc cctgtttccc tccctccgtc aggaggctcg
     1741 tggccacgac cctgtcgaat acctgcacgc ccagttgcag acattcgttg aagatggccg
     1801 gcttgaaggt ggcgccccag acacgaaact ggaacctgtt ctcgtagtcg tagcagtaga
     1861 cgaatttcgt cttctcatcc ctgaaaggag cccccttgaa cgtgtcgtcg atgtcccgta
     1921 ccttcgcgcc catcttttcg atctcgagaa gcgtgtcgta tgcctccctc gccgcaacgt
     1981 accgtgagag ggaattggag taccctttga acgtgaccct ctcccattcc acgcaatcct
     2041 cggctgttac cttcgatgcc gggttcggcg tgttgagcca gtggtcgaat ccggacccgc
     2101 cgccggacct tttcggatat gccttgtcca gcagggcaac cttcagcccc ttcttcgcgg
     2161 cgctgatggc cgccatgcac ccgcccgggc cgccgccgag cacaacgacg tccacgtcga
     2221 tatcgttctg tctgtcgtac ctgacaggat agggccattc gataacctga cccgtttgcg
     2281 ttaccgcatc ttcccagttc atattacctc cttcaagaac ttcgtggacc tgtcgtgatc
     2341 atcgtggtgc gcgttgagat aaaatgccgg aacgacctgt ctccccgcaa accgactgtc
     2401 tgtctcacgt tagtatggac taaccaacag accatgtgta attatcgacc aaagccggac
     2461 tgcttgtcaa ggaatatttg tgggaaagag aggggagttc ttccggggtc ctctcacggc
     2521 ccggtccgtc tacgtcacaa gcttcgtcgt gtgttccgtg tctctcgcca tgagttcgtc
     2581 gcagcgggca tagtccttct tttccatcgc tttgatgatc ccgtcatgac gtgcgtaggt
     2641 gtt
//
LOCUS       DAOPOA010000310         2637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_42307_length_2637_cov_3.441905, whole genome shotgun sequence.
ACCESSION   DAOPOA010000310 DAOPOA010000000
VERSION     DAOPOA010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42307_length_2637_cov_3.441905"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PKJ17_07120"
     CDS             complement(<1..897)
                     /locus_tag="PKJ17_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOD75778.1"
                     /translation="MEKAGTDLTKRSIWRTLDIPIIIAGFLVILPILGINRTTDFAIF
                     CIFVLAFNILYGFMGRLSFGHMLYLGTGAYAVTLFSEHVSGNPLLAILAALVAGALIG
                     VILGPIIVRTTGACFALINLAFCQVGYFLVLVAFSRYTGGEDGMSAYFSKMPLLNFGN
                     KGIVFGFVLFCLVLAYFFLKKFTASPYGVLIRSIKENETRVKFLGYNTFNYKWITFII
                     STTVSAFAGALSIINYGYVTPSFIDPSRAVEVIFAALIGGSGSLYGSIIGGVVYMAIS
                     NYLASYIPRWEMFLGFALLIVVF"
     gene            complement(988..1662)
                     /locus_tag="PKJ17_07125"
     CDS             complement(988..1662)
                     /locus_tag="PKJ17_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HOD75779.1"
                     /translation="MAVQNKMPKIDAKKTAIVLIEPQYDFLKPGGSMYQFIADQLEKR
                     HVIENLADLVAKARKKVKKIIYIPFEAFEPGFPEIDKEGPGMGGLRGLEIDMPTGWTL
                     HGKKVAGAWVAGTPGPEIIPELTPQKGDIIVRGKKTLDAFWSTALDYTLRVNMIEHVA
                     IVGFHTNWCVESTARSAYDHGYRVIVIGDCTGTDTDEEQNYAEKYIFPKIGHVMGYKE
                     FLKSLK"
     gene            complement(1726..2415)
                     /locus_tag="PKJ17_07130"
     CDS             complement(1726..2415)
                     /locus_tag="PKJ17_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD75780.1"
                     /translation="MLSVKNIDVAYGHARVLHDISLELEAGSMVFMAGRNGAGKTTFL
                     KSIAGFMKPIAGSISFLGRDISGMAPEKVALMGIRYVFQDKRVFSNLTVRENLELAAY
                     PVKEKTSHAIEKVMSIYPKMDKFLNLRAGSLSGGQRQILLIGRALVGDPKLLLVDEPT
                     EGLAAGTINDILNVLAVMKGKVSMIIVEQNLSVVGMLAEKVYIMREGKIVQTCTSKEE
                     IADKERLEQYL"
     gene            complement(2403..>2637)
                     /locus_tag="PKJ17_07135"
     CDS             complement(2403..>2637)
                     /locus_tag="PKJ17_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75781.1"
                     /translation="LSDSEIGEVSVIINRVKKDLSFVIIEHKISRIVDMVGRLCVMHE
                     GRLIADGPPNQVLCDATVREVYWGKEEGLTCSV"
BASE COUNT          503 a          764 c          797 g          573 t
ORIGIN      
        1 gaaaacgacg atgaggagcg cgaatccgag gaacatctcc catcttggta tgtaagaggc
       61 caggtagttg ctgatggcca tgtagaccac gccgccgatg atggacccgt agaggctccc
      121 cgaccccccg atgagggcgg cgaagatgac ctcgacggcc cgggagggat cgatgaagct
      181 cggcgtgacg tagccgtagt tgatgatcga gagcgccccg gcgaaggccg acaccgtggt
      241 ggagatgatg aaggtgatcc acttataatt gaatgtattg tatcccagga acttgacccg
      301 ggtctcgttc tccttgatgg accgtatgag cacgccgtag ggcgaggcgg tgaacttctt
      361 caggaagaag tacgccagca ccaggcagaa aaggacgaac ccgaacacga tgcccttgtt
      421 cccgaaatta aggagcggca tcttcgagaa ataggccgac atgccgtcct ccccgcccgt
      481 gtaccgggaa aaggcgacga ggacgaggaa gtagcccacc tggcaaaagg caaggttgat
      541 gagcgcgaag cacgctcccg tggtgcgcac gatgatggga cccaggatga cccctatgag
      601 ggcccccgcg acgagcgccg ccaggatggc gagcagcggg ttcccggaga cgtgctccga
      661 gaagagggta accgcgtagg ctccggtgcc gaggtagagc atgtgcccga aggaaagtct
      721 gcccatgaag ccgtagagga tgttgaaggc caggacgaag atgcagaaga tggcgaagtc
      781 cgtcgtcctg tttatcccaa gtatggggag gatgacgagg aacccggcga tgatgatggg
      841 gatgtccagc gtccgccaga tgctcctttt tgtgagatcc gttccagctt tttccatggc
      901 ctttttaagc ctccgtctcg catctcctga cccgctcccc cgtcgggaga gcgggtcagg
      961 agggtcatat ggtgcttaag tctcgcgtca cttgagactc ttgagaaatt ccttgtatcc
     1021 catcacgtgc ccgatcttgg ggaagatgta cttctccgcg tagttctgtt cctcgtccgt
     1081 gtccgtgccg gtgcagtcgc cgatgacgat gacgcggtag ccgtggtcgt aggcggaacg
     1141 ggccgtggat tccacgcacc agttcgtgtg gaagccgacg atcgccacgt gctcgatcat
     1201 gttgaccctg agcgtgtagt caagggccgt ggaccagaag gcatcgaggg tcttcttgcc
     1261 gcggacgatg atgtcgcctt tctgcggcgt cagttcgggg atgatctccg ggcccggcgt
     1321 accggccacc caggcaccgg ccaccttctt cccatgaagc gtccatcccg tgggcatgtc
     1381 gatctcgagc ccccgcaggc cgcccatgcc ggggccctct ttgtctatct ccgggaaacc
     1441 gggttcaaag gcctcgaagg ggatgtagat gatcttcttc actttcttgc gtgccttcgc
     1501 gacgagatca gccaggttct ctatgacgtg gcgtttttcg agctggtccg cgataaactg
     1561 atacatggaa ccgccgggtt tcaggaaatc gtactgcggc tcgatgagga cgatggccgt
     1621 cttcttcgca tcgatctttg gcattttgtt ctgtactgcc atgagctgcc tcctctttat
     1681 ggtctttttt atctatatct gcggcgtgcc gcccacttgc ctgactcaca ggtactgctc
     1741 cagccgctcc ttgtccgcta tctcctcttt ggacgtgcat gtctgcacga tcttgccttc
     1801 gcgcatgatg tataccttct cggcgagcat cccaacgacg gagaggttct gctcgacgat
     1861 gatcatggag accttgccct tcatcacggc aaggacgttc agtatgtcgt tgatggtccc
     1921 tgccgcgagg ccctccgtgg gctcgtcgac aagcagcagc ttcggatcgc cgacgagcgc
     1981 cctgccgata agcaggatct gccgctggcc gccgctcaag ctcccggccc tcaggttcag
     2041 gaacttgtcc atcttcgggt agatgctcat gaccttctct atcgcgtggg acgtcttctc
     2101 cttcacgggg taggcggcaa gttccaggtt ctcccggacg gtgaggttcg agaagaccct
     2161 tttgtcctgg aagacgtacc gtatacccat gagcgccacc ttttccggcg ccatgcccga
     2221 gatgtccctt cccagaaaag atatgctgcc cgcgatgggc ttcatgaagc cggcgatgga
     2281 cttgaggaag gtcgtcttcc cggcgccgtt gcggcccgcc atgaagacca tgctgcccgc
     2341 ttcgagttcc agggagatgt cgtggaggac ccgggcatgt ccgtaggcga cgtcgatatt
     2401 cttcacactg agcatgtcag tccctcctct ttgccccagt agacctcccg gacggtggca
     2461 tcgcagagca cctggttcgg cgggccgtcg gcgatgagcc ggccttcgtg cataacgcag
     2521 agcctgccca ccatgtcgac gatcctcgat atcttgtgtt cgatgatgac gaaactgagg
     2581 tccttcttga cgcggttgat gatgacggag acctcgccga tctcgctgtc ggagagg
//
LOCUS       DAOPOA010000311         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_42413_length_2632_cov_3.500194, whole genome shotgun sequence.
ACCESSION   DAOPOA010000311 DAOPOA010000000
VERSION     DAOPOA010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42413_length_2632_cov_3.500194"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PKJ17_07140"
     CDS             <1..697
                     /locus_tag="PKJ17_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HOD75782.1"
                     /translation="SRVLAAISRCPSIVVEPRVDTMLFKEVEFVRLSRNTILIVFVTT
                     SGMVHRRFVDTGEDMEQDVLDEMKGYMNERFSGMPFYALKENIIRDMRRDREDFQRLY
                     RKIQDAVDVLVGEEEKREIYIEGASKMIGIPEFSDVERLKDLFRALENKERLIQLLDR
                     YLSDEGINVILGSESDMKGIEGMSIITSAYRIGEDSYGLLGIIGPMRMNYSRLIPIVD
                     YTARAVTDLFRSM"
     gene            706..1299
                     /gene="grpE"
                     /locus_tag="PKJ17_07145"
     CDS             706..1299
                     /gene="grpE"
                     /locus_tag="PKJ17_07145"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOD75783.1"
                     /translation="MNEKDKDLLSEEKEVRHEEKGESGQKHEEHKKKKKKEELPSEVK
                     KELEEKDGKIRSLEERLLYLQADFENFKKIKAKEKQDLLKYANEVIIRELLPVIDNLE
                     LALKHAESTEDYKGIHEGVKITLNEFMKVLEKAGVKSIEAVGQKFDPNFHEAFYQEEH
                     EDVEPDTVVSEFQKGYLLNDRLIRPSRVGVSKKPEIQ"
     gene            1319..2419
                     /gene="dnaJ"
                     /locus_tag="PKJ17_07150"
     CDS             1319..2419
                     /gene="dnaJ"
                     /locus_tag="PKJ17_07150"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOD75784.1"
                     /translation="MRSRQHVDYFEVLNVPRGATDDEIKKAYRKLALQYHPDRNPGDH
                     SAEEKFKEINEAYEVLGDEQKRRHYERFGTVGDMGSVFDFGFQGNFDSVFNDLFSDFF
                     GNQRPRQRKGGDLRYNLTIEFEEAVFGGEKEIEIPREERCPVCSGSRVEPGHEPVICK
                     HCGGRGQVRQSHGFFTINRTCEYCGGEGQVIKHPCKNCKGRGTVRTKKTLKVRIPPGV
                     DNNSRLRVRGEGMQQPGDTIPGDLFVVLQVKEHAVFERAGDDIVVQVDVGFPLLCLGG
                     EITIPTIEGESVLKVPAGTQQGKVFRLKGLGVNKSNGYGRGDQLVYLNIVIPSDLTER
                     QKTLVEELAAEFRDSSPQTRKGFKEKFMEFFE"
BASE COUNT          679 a          664 c          807 g          482 t
ORIGIN      
        1 ctcccgcgtt ctcgctgcca tttcccgctg tccaagcatc gtcgttgagc cccgcgtgga
       61 caccatgctt ttcaaggaag tggagttcgt caggctttcg cgcaacacga tcctcattgt
      121 cttcgtgacg acatcgggaa tggtgcacag gaggttcgtc gatacgggcg aggatatgga
      181 gcaggacgtg ctcgacgaga tgaaagggta tatgaacgag cgtttttcgg gaatgccctt
      241 ctatgccctc aaggagaata tcatcaggga catgcgcagg gacagggagg atttccagag
      301 gctctaccgg aagatacagg acgccgtaga tgttctggtc ggcgaggaag agaagagaga
      361 gatatacata gaaggggcgt cgaagatgat aggtattccc gagttctccg atgtggagag
      421 gctcaaggac ctgttccgtg ccctcgagaa caaggagaga ctgatacagc tcctcgaccg
      481 gtacctgagc gacgagggca tcaacgtgat ccttggaagc gagagcgaca tgaaggggat
      541 cgaagggatg agcatcatca cctcggccta tcggataggt gaggacagct atggcctgct
      601 cgggatcatc ggcccgatga ggatgaacta ttcccggctt atccccatag tcgattacac
      661 tgcccgggca gtgacggatc ttttcagatc gatgtgagga agacaatgaa cgagaaagac
      721 aaagacctgc tcagcgagga gaaagaggta agacacgaag aaaagggcga gagcgggcag
      781 aagcacgagg agcacaagaa aaagaagaag aaggaggagc ttccttcgga agtgaagaag
      841 gagctggagg aaaaggacgg taagatacgg tccctcgagg agaggctgct ttacctccag
      901 gccgattttg agaactttaa gaaaataaag gcgaaagaga aacaagacct cctgaagtac
      961 gcgaacgagg tcattatcag ggagctgctc cccgtcatcg acaatctcga actggccctg
     1021 aagcacgccg aatccacgga ggattacaag ggcatccatg aaggcgtgaa gataacgctc
     1081 aacgaattca tgaaggttct tgagaaggcc ggcgtcaagt ccatcgaggc ggtgggccag
     1141 aagttcgacc ccaactttca tgaggcgttc taccaggagg aacacgagga cgtggagccc
     1201 gacacggtgg tctcggagtt tcagaagggt tacctcctca acgacagact cataagacct
     1261 tcccgggtgg gggtttccaa gaaacccgaa atacagtaag acgtaagaaa ggcgtaccat
     1321 gagaagcagg cagcacgtgg attatttcga ggtcctcaat gttccgcggg gggccacgga
     1381 tgacgagata aagaaggcat accggaagct tgccctccag taccacccgg accgcaaccc
     1441 cggggaccac agcgccgagg agaagttcaa ggagatcaac gaggcatacg aggtcctcgg
     1501 cgacgagcag aaaaggcgcc actacgagcg cttcgggacc gtcggcgaca tggggtccgt
     1561 cttcgatttc ggtttccagg ggaatttcga ctccgtattc aacgaccttt tcagcgattt
     1621 cttcggcaac cagcggccgc gccagaggaa agggggcgat ctcaggtaca acctgaccat
     1681 agagttcgaa gaggcggtgt tcggcgggga gaaagagatc gagatcccca gggaagagag
     1741 gtgtcccgtc tgcagcggtt cacgtgtcga gccgggccac gagccggtga tatgcaagca
     1801 ttgcggtggg cgcggacagg tccgtcagag ccacgggttc ttcaccatca acaggacctg
     1861 cgagtactgc ggaggagaag ggcaggtcat caagcacccc tgcaagaact gcaagggcag
     1921 ggggaccgtc aggacaaaga agacgttgaa ggtccggata ccccccggtg tcgacaacaa
     1981 cagccgcctc agagtgaggg gcgagggtat gcagcagccg ggagacacca tcccgggcga
     2041 cctcttcgtg gtcctccagg tcaaggaaca cgccgtgttc gagagggccg gcgatgacat
     2101 cgtcgttcag gtggatgtgg gattcccgct cctgtgcctg ggcggggaga tcaccatacc
     2161 caccatcgag ggcgaatccg tcctcaaggt accggcgggc acacagcagg ggaaggtgtt
     2221 caggcttaag ggcctcggcg tcaacaagtc caacggctat ggcaggggcg atcagctcgt
     2281 gtatctcaat atcgtcattc cctccgatct gacggagcgg cagaagacgc tcgtcgagga
     2341 actggccgcc gagttcaggg actcatcccc gcagacacgg aagggtttca aagaaaagtt
     2401 tatggaattc tttgaataaa aggacaggga cgggtcattg gtaataggtc atgggctgcc
     2461 gactgtctgt tcttcccata acctataacc cataacctat aacctgtctt catttcttct
     2521 ccgcattctc gaccatcagt tttgctatgg cctttgaaaa agccgccggg tctttcggtt
     2581 tcgatccctc cagcaccaac gcctggtcat agaggagccg gacataatcc tt
//
LOCUS       DAOPOA010000312         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_42420_length_2632_cov_2.730307, whole genome shotgun sequence.
ACCESSION   DAOPOA010000312 DAOPOA010000000
VERSION     DAOPOA010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42420_length_2632_cov_2.730307"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKJ17_07155"
     CDS             complement(<1..304)
                     /locus_tag="PKJ17_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75785.1"
                     /translation="MAIRNASSPSGWQPASLYVIPDGGGTEGLFYDAGRLVVRTATRS
                     NYYGGNYVGQAGQKVFGGASTNAAWVTVGNDATRFLLANGVTGSNVTTLLERGLGMD"
     gene            complement(666..>2632)
                     /locus_tag="PKJ17_07160"
     CDS             complement(666..>2632)
                     /locus_tag="PKJ17_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOD75786.1"
                     /translation="DFEVADAFGVTLALRFRSFMLRHLELVFVLLIAVLVCAIFFILP
                     YRFAFLNLFYLPVLLAAYILGKRKAFYASLNIIVIVGVTAVIRPEWFAVGGNVTSTLI
                     MIIIWGGFLVLTSAGIGSLQEKLAKGFEETRRLYEELKRSRVAEEMKEKVEKTLYTTM
                     DPVVAKLATEGKLRIEKREISIMFCDLTDFTAYSDQNPPDIVLDELNRFIGSIEPVIE
                     LFRGHIDKYMGDGVMVEFGAPVDYAQHALMAVLAAIKMQERVKDMGLPWKLRIGIATG
                     YTIVGMMGVHRQAYSAIGDRVNVAKRLEEICEPERIYVDEYTFNFVEPFVNVKKLRNM
                     GYSRQSDKALLEELRVLEHNLAEEGESADILYQMGKAHFGLHDASAAIACFERALALD
                     PDSTDLRLAYADANLKKDEFEKFQLKGKLHKIAVYEVRSIKSRWHDHTIIPRAVSERY
                     GDVEAAMKVPEDLVLAVESLDGSIGRGLLVGMLSYALADRLNLNEDLKLNIRQAGVLQ
                     DIGKEAVPHHILNRQASLTEQEIKFVEKYVLESVASLKRLGYVNDQFLEIVKHHHELW
                     NGNGYPDGLKGEEIPIGARITAVAEAYSAMTSWRPYRGPWDVRLALNEIRKNTEKGFY
                     DPQVVDVLFEILKFDASLVDRGEVSSLGSK"
BASE COUNT          523 a          780 c          774 g          555 t
ORIGIN      
        1 cgtccatgcc cagtccccgt tcaaggaggg tggtcacatt gcttccggtc accccgttgg
       61 ccagaaggaa cctggtggca tcgttgccga ccgtcaccca cgcggcattc gtcgaggccc
      121 ctccaaagac cttttgaccc gcctgaccga cgtaattgcc tccataatag ttggatctcg
      181 tggccgtacg aacgacaagc cttcccgcgt catagaagag cccctcggtg cctcccccgt
      241 cgggtatgac gtagagggac gccggctgcc agcctgatgg actggaggcg ttccgtatcg
      301 ccatctcgag ggcctcgctg tagctcggga tgaggtccgc cccggcggca ggaaaggtaa
      361 gacaaaggac agcacaaata acgaccaggg accacaggaa tgcacggtac cctcgacacg
      421 gcggggaagg gcgctttcgc accggtctcc caccatccgg gtatctcata gcccttcgcc
      481 tcccgaccaa tgatgtgcgc gcgcagggga atgagagatg tcctcgccgg gcgccgcaaa
      541 gatactacaa ggttttgtga cgattgtcca caccccggat agcgcagttc caggggaggt
      601 cggggcaggg tctgttggcg tgtttgttct tgaaatggga ggttcgtgcc gcccggcttt
      661 ggcgcctact tgctgcccag cgaggagacc tcgcctctgt ccaccaggga ggcatcgaac
      721 ttgaggatct cgaagagcac atcgacgacc tggggatcat aaaaaccctt ctccgtgttc
      781 tttctgatct cgttcaacgc aagacgaacg tcccaggggc cgcgataggg gcgccaggat
      841 gtcatggcgc tgtatgcctc ggcgactgcc gttatgcggg cgccgatggg tatctcctca
      901 cctttgagcc cgtcggggta gccattgccg ttccacagct cgtgatggtg cttgacgatc
      961 tccaggaact ggtcgttgac gtaaccgagc cgtttcaggg aggcgacgct ttcaagaacg
     1021 tatttttcca cgaacttgat ctcctgctcc gtcagactgg cctggcggtt caggatatgg
     1081 tggggtacgg cctctttgcc tatgtcctgc aggactccgg cctgccgtat gttcagtttc
     1141 aggtcttcat tgagattgag acggtccgcc agggcatagg aaagcattcc cacgagaagc
     1201 ccgcgcccga tggaaccgtc cagagactcg acggcaagga cgaggtcctc cggcaccttc
     1261 attgccgcct ccacatctcc gtaccgctcc gatacggccc gggggatgat ggtgtggtcg
     1321 tgccagcggc tttttatgct cctgacctcg tagaccgcga tcttgtgaag cttgcccttg
     1381 agctggaact tctcgaattc atccttcttg aggtttgcat cggcgtaggc aagcctgaga
     1441 tcggtggaat ccgggtcgag agcgagtgcg cgctcaaaac aggcgatggc ggcagaggcg
     1501 tcatggaggc cgaagtgtgc ctttcccatc tggtagagaa tgtcagcgct ctctccctcc
     1561 tcggcaagat tgtgctcgag gaccctgagc tcctcgagga gggccttgtc gctctgacgg
     1621 ctgtacccca tgttgcgcag cttcttcacg ttgacgaagg gttcgacgaa gttgaaggtg
     1681 tactcgtcca cgtatatccg ttcgggttcg cagatctcct caaggcgctt cgccacgttg
     1741 acgcggtcgc ctatggcgct gtacgcctgc cggtgcactc ccatcatgcc gacgatcgtg
     1801 tatcccgtgg cgatgcctat gcgcagcttc cagggaaggc ccatatcctt caccctctcc
     1861 tgcatcttta tggcggcaag cacggccatc agggcatgct gcgcatagtc gacgggcgcg
     1921 ccgaactcga ccatgacccc gtcgcccatg tacttgtcta tgtggccccg aaagagctct
     1981 atgacgggtt cgatggagcc gatgaagcgg ttcagttcgt ccagaacgat atctggagga
     2041 ttctgatcgg aataggcggt aaagtccgtc agatcgcaga acatgatgct gatctcgcgc
     2101 ttctctatcc tgagtttccc ctccgtggcc agctttgcca caacagggtc catcgtggtg
     2161 tagagggtct tctcgacctt ctccttcatc tcctcggcaa cgcggctgcg cttcaattcc
     2221 tcgtacaggc gccgcgtctc ctcgaaacct ttcgccagct tctcctgcaa cgatcctata
     2281 cccgcagatg tgagaacgag aaagccgccc cagatgatta tcatgatgag cgtgctcgta
     2341 acgttgccgc ccaccgcgaa ccattccggc ctgatgaccg ccgtgacgcc cacgataaca
     2401 attatgttga gggacgcgta gaaggccttg cgtttgccca gaatgtacgc ggcgagaagg
     2461 accgggaggt agaagaggtt gaggaaggcg aacctgtaag ggagtatgaa gaagatggcg
     2521 caaacgagaa cggcgatgag gagcacaaag acgagctcca gatgccggag catgaagctc
     2581 cggaaacgca gcgccaaggt aacgccaaag gcgtcggcta cctcaaaatc cg
//
LOCUS       DAOPOA010000313         2631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_42439_length_2631_cov_3.869953, whole genome shotgun sequence.
ACCESSION   DAOPOA010000313 DAOPOA010000000
VERSION     DAOPOA010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42439_length_2631_cov_3.869953"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..1088
                     /locus_tag="PKJ17_07165"
     CDS             369..1088
                     /locus_tag="PKJ17_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOD75787.1"
                     /translation="MKTKKVENEVPLPYFSDSPYVTDNVGKFTDELDTLLSIGRLRHY
                     SPGQIIYLQGEESTSFYFVRDGKVKVSIFKENGSEKILAIQEDNTFFGESAAFDRYPY
                     FATATAMEESNIYAFDIEETEALLKAHPEVSLLIITSIIRKLRLLGLQVEGLSFLDAQ
                     KRVASILLKLMHEVGEPTAGGILIKKRITHEDLANITGLSRVTVTNVLNYLDRLKLIT
                     KGRLKYTIIDRAKLESFVRSK"
     gene            1115..2068
                     /gene="dctP"
                     /locus_tag="PKJ17_07170"
     CDS             1115..2068
                     /gene="dctP"
                     /locus_tag="PKJ17_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOD75788.1"
                     /translation="MLYCLALAAVCLLPATQAHCTRLKTVTFAATNTVWVRQFHLFTD
                     AVQRDSKGEIRFQLVGGPEAIPPFEQIEAIRKGVVDMALLPAAYFVAQMPEADAMKLS
                     PHSPMEERKRGIFAFYRELMEKHLDVIYLGKVTGGISYHLYLRKPLKGPDLKGLKIRV
                     TPIYEPFVRALGGAGVTTAPGEVYVALERAIVDGFGWPSIGVQEMGWEEVVRYVIDPG
                     FYQTDVCVLMNRKTWDALPASEKKLIARAVEKAERETYDLSRKLAGQERQLLLSRGVQ
                     AIEFRDNEKEFYLDTAYRAGWEKVIAKSPVIGGHLRKLMGR"
     gene            2070..2546
                     /locus_tag="PKJ17_07175"
     CDS             2070..2546
                     /locus_tag="PKJ17_07175"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOD75789.1"
                     /translation="MRRLLSRTLDAAAVFSAACIAFIALSISCEVIARYFLHRPLRWT
                     VEISEYLQIYFSFLSAAWVLRKKGHVSLDIVVVRLGPRGRKAFRLATDILSIVVAATL
                     CLFSALVAREQLLLGTPVIKSLEVPKWIVIAPIPLGMLLVTAESFVKLIDDLRGGG"
BASE COUNT          574 a          774 c          715 g          568 t
ORIGIN      
        1 aacccgcgac ttcaaccttg gcaaggttgc actctaccgc tgagttattc ccgcacgtgt
       61 tgtttcttat aatacaaacc cctccccgat gtcaagacgg aaaagagtgg tcgcctcgga
      121 gccgcgacgc aaaaaaaacc ggaggagacc cggataatct cccccggcat tcagcaggca
      181 agccagctac cctgcatctt cattcatcgt taaggcgacc cggggtccac gccctttgtc
      241 ggccctttat cgtaccggcc cgtaatccct ttcctgagaa cctttcccga ccgttcgaca
      301 catctatgat atacggcctc ctgtcatttt tctgtaaaac aggctacatt cgtgctagtt
      361 tttataggat gaaaaccaag aaagtcgaaa acgaggttcc cctgccctat ttttccgatt
      421 caccctatgt cacggataat gtcggcaaat tcacggacga actggacact cttctctcca
      481 tcgggcgcct caggcactac agccccggtc agatcatata cctgcagggc gaggagagca
      541 cgagcttcta ctttgtcagg gacgggaagg tcaaggtgag cattttcaag gagaacggtt
      601 cggagaagat actcgccata caggaggaca acaccttttt cggagagagc gcggccttcg
      661 atcgctaccc ctatttcgcc acggccacgg ccatggagga atcgaacatc tacgccttcg
      721 atatcgagga gaccgaggcc ctgctcaagg cgcatcccga ggtgtcgctg ctcatcatca
      781 cctccatcat cagaaagctc agacttctcg ggctgcaggt ggaaggcctc tctttcctgg
      841 acgcgcagaa acgtgttgcc agcatcctcc tgaagctcat gcacgaggtc ggcgaaccca
      901 ccgccggcgg cattctcata aagaagagga tcacccacga agaccttgcc aacatcacgg
      961 gcctctcgcg ggtaacggtg acgaatgttc ttaactacct ggacagactg aagctcatca
     1021 cgaaagggcg cctgaagtat accatcatag atcgcgccaa gctcgagtcc tttgttcggt
     1081 cgaaatgagc aggacgccta tcatgttccg gagcatgtta tactgccttg ccctggcggc
     1141 cgtctgtctc cttcctgcca cgcaggcgca ttgtacccgc ctgaaaacgg tcaccttcgc
     1201 agcgacaaac acggtctggg tgagacagtt ccatctcttc accgacgccg tacaacgcga
     1261 cagcaaaggg gagatccgtt ttcagttggt gggggggccc gaggcgatcc cgccttttga
     1321 acagatcgag gcgatacgaa aaggcgtcgt cgatatggcc cttctgccgg cggcctattt
     1381 cgtggcccag atgcccgaag cggatgccat gaagctttct ccccattctc ccatggaaga
     1441 acgaaaaagg gggatcttcg cgttctacag ggaactgatg gagaaacatc tcgacgtcat
     1501 atatctcggg aaggtaacgg gggggatcag ttatcatctc tacctcagga agcctttaaa
     1561 aggacctgat ctcaagggtc ttaagatcag ggtcaccccc atttacgaac ccttcgtcag
     1621 ggcccttggc ggagccgggg tcacgacggc tcccggtgag gtctatgtcg ccctcgaaag
     1681 ggccatagtg gatggtttcg gctggccgtc catcggcgtt caagagatgg ggtgggaaga
     1741 ggtggtccgc tatgtcatag accccggctt ctaccagacc gatgtctgcg ttctgatgaa
     1801 caggaagacc tgggacgctc ttcccgccag cgaaaaaaag ctcatcgccc gggccgtcga
     1861 gaaagcagag cgtgagacct atgacctttc caggaagctg gcagggcagg aaagacagct
     1921 tctcctctcc cggggcgtac aggcgataga gttcagggac aatgaaaagg agttttacct
     1981 cgacacggcc taccgggcgg gctgggagaa ggtcattgca aagagtcccg tcatcggggg
     2041 gcatctcaga aagctgatgg ggcgatgatg tgaggcgcct cctttccagg accctcgacg
     2101 ccgcggccgt attctccgcc gcctgcatag cgttcatcgc cctgtccata tcctgcgagg
     2161 tcatagcaag gtatttcctg caccggcccc tcaggtggac cgtcgagata tcggaatacc
     2221 tgcagatcta cttttccttt ctcagtgccg cctgggtcct gaggaaaaaa gggcatgtaa
     2281 gcctggatat cgtggtggtc cggctcggcc ccaggggcag gaaagccttc aggttggcaa
     2341 cggacatact ttccatcgtc gttgccgcaa cgctgtgcct cttctcagcc ctcgtcgcac
     2401 gcgaacaact ccttctcggg acacctgtca tcaagtcgct ggaagtgccg aaatggatcg
     2461 tcatcgcacc cataccgctg ggcatgctcc ttgttacggc tgaatccttc gtcaagctca
     2521 tcgacgacct gcggggcggg ggctgacgtg gagtggtggg tcgtactcct cgtcttcttc
     2581 ggcggtttca tcggcctgct cgtgaccggt ctccccgttg ccttcgcctt c
//
LOCUS       DAOPOA010000314         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_42534_length_2627_cov_3.706843, whole genome shotgun sequence.
ACCESSION   DAOPOA010000314 DAOPOA010000000
VERSION     DAOPOA010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42534_length_2627_cov_3.706843"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKJ17_07180"
     CDS             <1..522
                     /locus_tag="PKJ17_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75790.1"
                     /translation="PAEVREETEEIVPAPPRTEEPVMAAPPPPPPPPPTERPVMAAPP
                     PPPPPPPPPPPPPPPPPPVPEEPVMAPPPPPPPQEVSGDLAQAFAEDVREQAEAEPDV
                     AVIAGCRIKIEIPSHIAEQTVGAGVDQKRKVAGVLAAQTLEKNPQLTASPTVDPVSIW
                     SEILTVIMKRLEA"
     gene            complement(736..1680)
                     /locus_tag="PKJ17_07185"
     CDS             complement(736..1680)
                     /locus_tag="PKJ17_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD75791.1"
                     /translation="MATAKKTAQQWFLEAYHHHLLEQSIDKAIKAYKKCIQLDPAYAD
                     AYVNLGLIHLKREQYEKALQYFARVTQLEPDNIEAYINLGYAYEKMDRFGSAKQMYEH
                     SLKINPRHMEAFINLANIAEMQADYNGAIELWKKAIEVDPNAAQPHFFLASTYDRHDM
                     FDEAIEEYGQTLAIDPHHLKALFNLGRLYIQQGEPRKAFEPFARILELNGDNTAAWNY
                     LGYIYETLGSIEYAIFAYSNSLIINNYQEDAHYNLARLQYLQYRVSPDPVMLEGIRSR
                     LLYVTSVNPKHREAKQLLGMIQLHRTREQKETENQEMA"
     gene            complement(2002..>2627)
                     /locus_tag="PKJ17_07190"
     CDS             complement(2002..>2627)
                     /locus_tag="PKJ17_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOD75792.1"
                     /translation="RGAFRKRPDICQGGEAGMIDTFLLSFIPLFVAFDVLGVLPIYIR
                     FTASMDTTKRTRHLRDSLLTAFTISLLFVIVGNRVMTYLGISMQDFLIAGGIVIFITA
                     IRDLIQGRSEPAGENEFFGVVPLGIPLLAGPAVLATSMIIWNQYPFYYYLISLVLNII
                     ICGAVLFFSDFILKLLGKRVVEALSRVFSLIIASIAIMFIRKGIQGF"
BASE COUNT          557 a          688 c          805 g          577 t
ORIGIN      
        1 ccggctgagg tgagagaaga gaccgaagag atcgtgcctg ctccccctcg aaccgaggag
       61 cccgtgatgg cggcaccccc gccgcctcca ccgccaccac ctacggaaag gcccgtgatg
      121 gcggcaccgc ctccacctcc gcctccacct ccacctccgc ctccgcctcc accacctcct
      181 ccaccggtac ccgaggagcc cgtgatggcg ccgcctccac ctcctccacc tcaggaggtg
      241 tcgggagatc ttgctcaggc attcgcggaa gatgttcggg aacaggcaga ggcagagccg
      301 gatgtggcgg tcattgccgg atgcaggatc aagatagaga taccttctca tatcgcggaa
      361 cagacggtgg gcgcgggtgt tgatcaaaag agaaaggtag caggcgttct ggccgcacag
      421 acgctggaga agaaccccca gctcacggcc tcgccgacgg tcgaccctgt ctccatctgg
      481 agcgagatac tgacggtcat catgaagagg ctggaagcat agcgccgcaa cccgttcggg
      541 tcgcggcggc ttgaaccgct tgaccgttaa aaccgcttga acgttggaaa cctgaacccc
      601 catcagtaag gaatgatggg ggtttttgcc tgtattcgtc ccggcacggc attacgcgcg
      661 gaccgaggac aatgatgcgt agacgccgga ggtgaagttc tttaacgctc gagcagttca
      721 agcggctcaa gctgttcaag ccatttcttg gttttccgtt tctttctgtt ccctcgttct
      781 atgcagctgg atcatgccga ggagttgttt ggcctcgcgg tgcttggggt tcacggaagt
      841 gacgtagagg agcctgcttc gtatcccttc aagcatcacc gggtcgggac tcacgcggta
      901 ctggaggtat tgcagccgcg ccaggttata gtgggcgtct tcctggtagt tgttgatgat
      961 gagggagtta ctgtacgcga agatggcgta ctcgatgctt cccagcgtct cgtagatata
     1021 tcccaggtaa ttccacgcgg ccgtgttgtc gccgttcaat tccaggatgc gtgcgaaggg
     1081 ctcgaaggcc ttcctcggct caccctgttg tatatagagg cgcccgaggt tgaagagggc
     1141 cttcagatgg tgggggtcga tggcaagggt ctgcccgtat tcctcgatgg cctcgtcgaa
     1201 catgtcgtgc cggtcatacg ttgaagccag gaagaaatgg ggctgagcgg cgttgggatc
     1261 gacctcgatc gccttcttcc acaactcgat ggccccgttg tagtcggcct gcatctcggc
     1321 gatgttggca aggttgatga aagcctccat gtgccgggga tttatcttca gggagtgttc
     1381 atacatctgt ttcgcgctgc cgaagcggtc catcttctcg taggcatagc cgaggttgat
     1441 gtatgcttct atgttgtccg gttccagctg ggtcacgcgc gcgaaatact gcagggcctt
     1501 ctcgtattgt tcccgcttga gatggatgag tccgagattg acataggcgt ccgcgtaggc
     1561 ggggtcgagc tgtatgcact tcttgtacgc cttgatggcc ttgtcgatgg actgttcaag
     1621 caggtggtga tggtaggctt caaggaacca ttgctgggca gtcttcttcg cggtggccat
     1681 tcgatctccc gcactcacgc atgaggcaac aacacagtag caaagcggcg ccgacgtgtc
     1741 aagacaaaga agaccggtgt caggtctcac ggtccaggtt ttaaagttca tgggaaagaa
     1801 aaatggttcg ctcttgcgcc ggaccctgga acccttgaac tgtctgtaaa ctgtacagtc
     1861 cagacggtac agtttaaaat cagctacaat tcttgtcaaa acatcttcga tttcatctgg
     1921 aatatgacag tattgcgcaa aggtggacta tcaggtgtgt cgtgggaacg attcaagcgg
     1981 ttcaagccat tcaagccgtc tttaaaaccc ctgtatcccc ttccgtatga acatgatggc
     2041 gatggaggct atgatcaggc tgaagacacg ggagagggcc tctacgacgc gtttgcccag
     2101 gagtttgagg atgaagtcgg agaagaagag gacggccccg caaatgatga tgttgaggac
     2161 gagggagatg aggtagtaat agaaagggta ctggttccat atgatcatgg aggtggcgag
     2221 gacggcgggg cccgccagaa ggggtatgcc cagtggcacg accccgaaga actcgttctc
     2281 cccggccggt tcggagcggc cctggatgag gtctctgatg gcggttatga agatgacgat
     2341 gcctccggca atgaggaaat cctgcatgga tatcccgaga taggtcatga ccctgttgcc
     2401 gacgatgacg aagagcaggg atatggtgaa tgccgtgagc agggagtccc tgaggtgcct
     2461 tgttctcttt gtggtgtcca tggaggcggt gaaccggatg tagatcggca ggacccccag
     2521 gacgtcaaag gccacgaaga ggggaatgaa ggaaaggagg aaggtatcta tcatgccggc
     2581 ttcccctccc tgacatatat ccgggcgttt tcggaaagcg ccccgtc
//
LOCUS       DAOPOA010000315         2614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_42842_length_2614_cov_3.643611, whole genome shotgun sequence.
ACCESSION   DAOPOA010000315 DAOPOA010000000
VERSION     DAOPOA010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42842_length_2614_cov_3.643611"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKJ17_07195"
     CDS             complement(<1..415)
                     /locus_tag="PKJ17_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75793.1"
                     /translation="MIARHDMKKPRMDRGVALVSALIVVSVAAGIFAAVMYYAMTGSE
                     LSGLQRKYQSSKEASLGAIEIFTKDVLPRVVSGTELSAAVTGMVVPSVMPTIQADAGK
                     DACFRAKLTSVTSAWPGGTCDSGPDSTLNSDIVFNL"
     gene            complement(438..1553)
                     /locus_tag="PKJ17_07200"
     CDS             complement(438..1553)
                     /locus_tag="PKJ17_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD75794.1"
                     /translation="MTSDKKGFSLLEILIAIAIMGIIMAMNSQLLMDLVRGQRQQAGV
                     VTTQFETSLGLEMLRSDVSNAGFGLPDEFASAPGTYTEATADPAQQFNSAPNVPRALM
                     HSNNVGAFTNYIANSDYLVIRSPAVGMNTAAGKWTNITSTTVHVWNDVNLDMVNGRDY
                     MIVIKPRSALGGRSKLIVSSGAYSLQYITSALDAAFQPSAATGERYLAFGVEDSIAPT
                     MPFNRADYFVKRPAVTNVNCAPGTGTLYKAIVKHGPAGTGGATEECPLVECVANMQVV
                     FRLDTNLDGVPDQTVTDISGLDALSIKEQVKEVQIYILGHEGILDRRFTYGGANPVTV
                     GPSAAMGTTVNLTGFGSDWNHYRWKIYTLIVKPKSFY"
     gene            complement(1567..1986)
                     /locus_tag="PKJ17_07205"
     CDS             complement(1567..1986)
                     /locus_tag="PKJ17_07205"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD75795.1"
                     /translation="MLRHVTKNRKGFTLIELLVSMLVLSIGLMAMLDGLANYIRINME
                     NQMRNEAMRIAESTLETLRNARFSDVQSHAVVITTPEVRRVRNIDVSYAVTWTVTNVA
                     ATGVTTPTSVAVQVSVTWNHRNVAHRHDAASIISTDA"
     gene            complement(1967..2530)
                     /locus_tag="PKJ17_07210"
     CDS             complement(1967..2530)
                     /locus_tag="PKJ17_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD75796.1"
                     /translation="MRDRRGFTLVELLIVIAIVGILAAIAAPNMSGFVRQNRIRNQTI
                     RIYNDIMNTRIMAMNTNRTHFIEFGLAGNQYRVVEDTNGNNANNAGGADTTRLARTAI
                     IPFTWSNTDPGMEAIEIQAGAFTNNFVTFESRGVATGLLNVSGGAICIPSVNLNPSTN
                     CIVVTPTRIRMGKYSGAAGGCSAAACN"
BASE COUNT          490 a          778 c          749 g          597 t
ORIGIN      
        1 tcaggttgaa gacgatatcg ctgttgaggg tggagtcggg tccgctgtca caggttcccc
       61 caggccaggc cgatgtgacg cttgtcagct tggcgcggaa acaggcatcc ttccccgcgt
      121 ccgcctggat agtcggcatg acgctgggga ccaccatgcc cgtgacggct gccgagagct
      181 ccgttcccga taccacacgg ggcaggacat ccttggtaaa aatctctatg gcaccgagag
      241 aagcctcttt tgaactctgg tactttctct gcaaacccga aagctccgac cctgtcatcg
      301 cgtagtacat gacggccgca aagatccctg cggcaacaga gacaactatc aatgccgata
      361 cgagggcaac gccgcggtcc atcctgggct ttttcatatc gtgccgggct atcattctgt
      421 ccccttccaa tccctctcta gtagaagctc ttgggcttca cgatcaacgt gtatatcttc
      481 caccggtaat ggttccaatc ggagccgaag ccagtgaggt taacggtcgt gcccatcgcc
      541 gcggaaggtc cgacggtgac gggattggct ccgccgtacg tgaacctgcg gtccaggatt
      601 ccctcgtgac cgagtatgta tatctgcact tccttgacct gctccttgat ggagagggca
      661 tccaaccccg atatgtccgt caccgtctga tcgggaactc catccaggtt ggtatcgagc
      721 ctgaagacga cctgcatatt tgcgacacat tcaacgaggg gacactcttc cgtcgccccg
      781 cccgtcccag cgggcccatg cttgacgatg gccttgtaga gggtccccgt gcctggcgcg
      841 cagttgacgt tcgtgacggc gggccttttg acgaaataat ccgccctgtt gaagggcatg
      901 gtcggagcga tgctgtcttc cacgccgaag gctagatacc gctcccccgt cgccgcggaa
      961 ggctggaagg cggcatccag ggccgatgtg atgtattgca gggaataggc gccggaagaa
     1021 acgatgagct tggacctgcc ccccagggcc gagcgcggct ttatgacgat catgtagtcc
     1081 cgcccgttca ccatgtcgag attgacgtca ttccagacgt gcaccgtcgt actggtgatg
     1141 ttcgtccact tcccggcggc ggtgttcatg ccgaccgcgg gagacctgat cacgaggtag
     1201 tcggagttgg cgatgtaatt cgtgaacgcc cccacgttat tgctgtgcat gagggccctc
     1261 ggcacgttgg gagcgctgtt gaactgctgg gcggggtcag ctgtggcctc cgtgtatgtc
     1321 ccgggggcgc tcgcgaattc atccgggaga ccaaaccccg cgttggacac gtcgctcctg
     1381 agcatctcca gcccgaggga ggtttcaaac tgggttgtga caacaccggc ctgctggcgc
     1441 tgtcccctga caagatccat aaggagctga ctgttcatcg ccatgatgat ccccatgatc
     1501 gctatggcga tgagtatctc caacaggctg aaccctttct tgtcgctcgt catagatccc
     1561 ttcacctcat gcgtcggtgc tgatgatgga cgccgcgtcg tgccggtgcg cgacattcct
     1621 gtggttccag gtgacactca cctggaccgc tacgctggtc ggtgtcgtta cacccgttgc
     1681 ggcgacattc gtcaccgtcc aggtgacagc ataggacacg tcgatgttcc ggaccctcct
     1741 gacctcaggg gtggtgatca ccaccgcatg gctctgcaca tcgctgaacc tcgcgttacg
     1801 aagggtctcc aaggtagact cggcgatgcg catcgcttcg ttgcgcatct ggttctccat
     1861 atttatcctt atgtagttcg ccaggccgtc gagcattgcc atgagaccga tggagaggac
     1921 cagcatggac acgagaagct cgatgagcgt gaaacctttt ctgttcttag ttacatgccg
     1981 cagcactgca ccctcccgct gctccgctgt atttccccat cctgatcctc gtgggcgtga
     2041 cgactatgca gttcgtgctg ggattgaggt tcaccgaagg tatgcagatc gcgcctcccg
     2101 acacgttaag caatcccgtc gcgacaccgc gggactcgaa ggtaacgaag ttgtttgtaa
     2161 aggctcccgc ctggatctcg atcgcttcca tcccggggtc ggtgttggac cacgtgaagg
     2221 ggattatggc cgtcctcgcg agccgtgtcg tatcagcgcc ccccgcgttg ttcgcgttgt
     2281 tcccgttcgt gtcctccaca acgcggtact gatttccggc gaggccgaac tcgatgaaat
     2341 gggtacggtt cgtgttcatc gccatgatcc tggtgttcat gatgtcattg tagatccgta
     2401 tcgtctggtt ccggatcctg ttctgcctca caaaaccgga catgttcggg gcggcgatgg
     2461 cggcgaggat ccccacgatg gcgatgacga taagaagctc gacgagtgtg aagcctctgc
     2521 gatctctcat ttttccttca tatggagtat ttttctcaac ggtttcggag gacccagaag
     2581 cgagaaacct ccgccgatgt tcacaccctg accg
//
LOCUS       DAOPOA010000316         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_42994_length_2607_cov_3.474922, whole genome shotgun sequence.
ACCESSION   DAOPOA010000316 DAOPOA010000000
VERSION     DAOPOA010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42994_length_2607_cov_3.474922"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PKJ17_07215"
     CDS             complement(<1..663)
                     /locus_tag="PKJ17_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOD75797.1"
                     /translation="MLDALTSFDRFLAVYLIPVGWKVLGAIVLWIVGSWLIKIIGNLS
                     GRGMRAHKLDPTLVKYFDGTIRIILKIVLIIAILSIFGIQTTSFVALIAAAGIAIGAA
                     WGGLLANFAAGAFLIILRPFHVGDMISAAGVTGDVREIGLFATTIDTLDNLCVVVGNN
                     KVLSDNIINYTRNPFRRVDLKAQLAHGVDPREAADRLMERIKNIPNVVDTPEPCVEIL
                     EFN"
     gene            954..1796
                     /locus_tag="PKJ17_07220"
     CDS             954..1796
                     /locus_tag="PKJ17_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOD75798.1"
                     /translation="MARKVRGALSLLWQALLQFYDDNGFFLSSGITFNIFITLVPFII
                     LLLAVVGSYLHNDQEVLGHMRAYLRSAAPAMDPGIMNDLMNVIERRQAIGIAGFAGLV
                     WFSTWLFASLRVALHIVLKVARPRGIVHAFGIDLLMVGAVGVLFLMNMALSSFFAFLQ
                     SHQEWIPALAGPAIRLTLRYLLPFFLTFCVFSLIYKVIPNEVVNLRSTLKVSLLTSLF
                     WEAAKHFFAWYVSNIAEYSLVYGSLSTLVVFVLWIYYSSTIFVVGGELLYLLEKERKA
                     VPVD"
     gene            complement(1936..>2607)
                     /locus_tag="PKJ17_07225"
     CDS             complement(1936..>2607)
                     /locus_tag="PKJ17_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOD75799.1"
                     /translation="FHAPGSYLRVTLKDNWRLDREAMRAIVKVGGNAIVEQASVRVGF
                     FIYTRLMYSLGVTMFAAHNIAMQFLALTFNFADGLSVAATSLVGQNLGRGRRDLSLLY
                     GKVTQRVALALDSLLDKMEIKVESNVLFTDKALDEIKQLMVAVGAEFTDVKDFCFTRN
                     PVLKKQIESDLDKVTKMIDEFDHVHQNRLITGVCMPQASYLYIDMTDSLKRMSRELAA
                     FANTF"
BASE COUNT          500 a          726 c          792 g          589 t
ORIGIN      
        1 gttgaactcg agtatctcga cgcagggctc cggggtatca acgacgttgg gaatgttctt
       61 tatcctctcc atcaggcggt ccgccgcttc ccgggggtcg acaccgtggg cgagctgcgc
      121 cttgaggtcc acgcgccgga aggggttgcg ggtgtagttg atgatgttgt cggagaggac
      181 cttgttgttg cccacaacga cgcacaggtt gtccagggta tctattgtag tcgcgaatag
      241 accgatctcc cgcacgtctc ccgtgacccc ggcggcggat atcatgtctc ccacgtgaaa
      301 gggtctgagg atgattagga aggctccagc cgcgaagttt gccaggagac cgccccaggc
      361 ggcgcctatg gctatgccgg cggcggcgat gagggccacg aaggacgtgg tctggatccc
      421 gaagatggag aggatggcta tgatgaggac tatcttcagt atgatgcgga tcgtcccgtc
      481 gaaatacttc acgagggtgg ggtccaactt gtgggcccgc atgccccggc cggacaggtt
      541 gcctatgatc ttgatgagcc agcttcccac gatccagagc actatcgcgc cgaggacctt
      601 ccaaccgacg ggaatgaggt aaacagccag aaaacggtca aaactggtca atgcgtccag
      661 cataacgacc tcctgtcatg tgtgtttgtc tttcatcgac cgttgtgttc gatcttattt
      721 cagtatatca ccccggacgt caacggagga ggtcgcagta tcgccccacc gcctcggggc
      781 actacagaca aggttgcgcg ggtttcgcgg ttgtcacgtt cgctgaaaat ggtttgattg
      841 ttggtccaag atatgaaaaa gttaatgagg aaaaccgtgc ggcccgtgtt gttggcccgg
      901 cctagggaga aaggaagaca atggcgcgaa ggtgtgggaa gaggctcgga tctgtggcga
      961 gaaaggtacg gggagccctg agccttctgt ggcaggcact gctgcagttc tatgacgaca
     1021 atggtttttt cctgtcgtcg ggcatcacgt tcaacatctt cataaccctg gtccccttca
     1081 tcattcttct tctggccgtt gtggggagct accttcacaa tgaccaggag gtgctgggcc
     1141 acatgcgcgc ctacctgagg agcgcggcgc ccgccatgga ccccgggatc atgaacgatc
     1201 tcatgaacgt gatcgagagg cgccaggcca taggcatcgc cggattcgcc ggactggtgt
     1261 ggttctcgac gtggctcttc gcgtccctga gggtggccct gcacatcgtg ctgaaggtgg
     1321 caaggccccg cggcatcgtg cacgccttcg ggatcgacct ccttatggtc ggcgcggtgg
     1381 gcgtgctgtt cctcatgaac atggcactga gttccttctt tgcttttctc cagagccatc
     1441 aggaatggat cccggcactc gcgggaccgg ctatccggct gacgttgaga tacttgctgc
     1501 ctttcttcct taccttctgc gttttctctc tgatctacaa ggtcattccc aatgaagtgg
     1561 tgaatctccg gtcgaccctg aaggtctctc tcctgacgag ccttttctgg gaggcggcaa
     1621 agcatttctt cgcctggtat gtcagcaaca tagccgagta ttccctggtc tacggctcgc
     1681 tgagcaccct cgtcgttttt gtcctgtgga tctactattc ctcgacgatc tttgtcgttg
     1741 gaggggagtt gctctatctg ctggaaaagg agcgaaaggc cgtgccggtg gactaaagaa
     1801 ggtcgctcat ggcccccggt tctgaaacag aaaggagcga ggtccgccgc ggggatcagg
     1861 gtccgggacg atatcccgaa accccatccc ggctgccggg ttgcccccgg aatgtttgaa
     1921 tgtgcgaacc aggtttcaga atgtgttggc gaaggccgcc agttcccgcg acatcctctt
     1981 caaggaatcc gtcatatcga tgtacagata cgaggcctgg ggcatacaca cgcccgttat
     2041 caggcggttc tgatggacgt ggtcgaattc gtctatcatc ttcgtgacct tgtccaggtc
     2101 gctttctatc tgcttcttca gcacaggatt cctggtgaag caaaagtcct tcacgtcggt
     2161 gaactccgcg ccgacggcca ccatgagctg ttttatctcg tcaagggcct tgtcggtaaa
     2221 gagcacgttg gattcgacct ttatctccat cttgtcaaga aggctgtcga gcgccagggc
     2281 gacgcgctgg gtgacctttc cgtacagcaa gctcaggtcg cggcgccccc ggcccaggtt
     2341 ctggccgact agggaggtgg cggcgacgga caggccgtcg gcgaagttga acgtcagcgc
     2401 caggaactgc atggcgatgt tgtgcgcggc gaacatcgtc acgcccaggg agtacatcag
     2461 ccgcgtatag atgaaaaaac ccacgcgaac gctggcctgc tcgacgatgg cgttgccgcc
     2521 caccttcacg atggcgcgca tggcttcgcg gtccagccgc cagttgtcct tcagcgtcac
     2581 gcgcaggtag ctgcccgggg cgtggaa
//
LOCUS       DAOPOA010000317         2603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_43092_length_2603_cov_3.082810, whole genome shotgun sequence.
ACCESSION   DAOPOA010000317 DAOPOA010000000
VERSION     DAOPOA010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43092_length_2603_cov_3.082810"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..>503)
                     /locus_tag="PKJ17_07230"
     CDS             complement(163..>503)
                     /locus_tag="PKJ17_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOD75800.1"
                     /translation="SPQPSSRVRDVMRNPVNELFLSVASAWEIVIKVNLGRLRLPDRP
                     DRFIPGQLMKNAIEPLPVEMSHALYVSRLPAIHRDPFDRTIIAQSILEKMPVVTRDAD
                     IAKYKTKTLW"
     assembly_gap    504..513
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(548..772)
                     /locus_tag="PKJ17_07235"
     CDS             complement(548..772)
                     /locus_tag="PKJ17_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOD75801.1"
                     /translation="MNIHEAKTRLSELLNRVESGEGSTIARAGTPIARLVPIRDKTAE
                     RVAGTAKGKVVIEKNFDEPLPESILGEYEG"
     gene            complement(809..1897)
                     /locus_tag="PKJ17_07240"
     CDS             complement(809..1897)
                     /locus_tag="PKJ17_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOD75802.1"
                     /translation="MSNFLDDIIDRAISEDASDIYIQSESPVFLRISGRMAAAADPPE
                     GYEIDEAIERILRVSGARAQLYDREEKDLSYARQVGSGKEKKTFRFRVNLCMTKRGVS
                     IVMRRLKSVETNPLNLGVPGDLLRLVLESPTGGIIFIAGPTGSGKSTTLASLIAYLVA
                     TRCLNCVTIEDPIEYDIPEGMGNIIEREVGTTTLSFERALRAAMRQRPDVILVGEVRD
                     PETASAAIQAAKTGHLVFTTIHTLMTTQVPSRIASMFPVEKQEWMVQELTDVLLAVMA
                     QVLVPTAGTDRQRGRLVLATEFLDTKDAAIRELLVRGRYDLLRQQQRSGQIGWTLNQS
                     LRVLIDSEEVEEADARRATNDPALFMRE"
     gene            complement(1901..2221)
                     /locus_tag="PKJ17_07245"
     CDS             complement(1901..2221)
                     /locus_tag="PKJ17_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75803.1"
                     /translation="MEPGTRHDYQNINSAIIVPRLNISLEDLIICICPAMLGNGIASI
                     LSYVSEEIGVFLITTGMCGSVALYFFARGKRKEEVRQRGCSRSGVLRRYLAYLLGAEW
                     LRCR"
     gene            complement(2228..>2603)
                     /locus_tag="PKJ17_07250"
     CDS             complement(2228..>2603)
                     /locus_tag="PKJ17_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75804.1"
                     /translation="KIRAGKLGSLCRFFLSAALCLLFGAVRTGDGWAQTFNESRWATE
                     TTDAGEFLSNLANIIMYGLLSISFCAGTGLIIWGFVVMANKDNNPQGAQGAWKKVAFG
                     GCLVSTSAILGIIKATMTASAG"
BASE COUNT          514 a          794 c          706 g          579 t
ORIGIN      
        1 ctaagtgttc ccaggatacg gcactgcgcg atatcctcga tcttgtcgca aggggaatcc
       61 tggtgaagga cccgggcggc ggccgcagca cgagctactc tctaaggaac gacacatcgt
      121 ggtgacccgt ctccgatcaa gtcccggtcc tgttaacacc cgtcaccaga gagtcttcgt
      181 cttgtatttg gctatatcgg catcccgcgt gacaacaggc atcttttcca gaattgactg
      241 ggctatgata gtgcgatcga agggatcccg gtggatggcc ggaagccggg acacatagag
      301 cgcatggctc atctcgacag gaagcggctc gatagcattc ttcatcagct ggcccggaat
      361 gaatcgatca ggtctgtccg gcagacggag ccttcccaga ttgaccttta tgactatctc
      421 ccaggcggaa gccacactca ggaagagctc gttcaccgga ttcctcatga catcacgcac
      481 gcgagatgac ggttgcgggc tatnnnnnnn nnncaccaga gaaacacatg agtgtcaagg
      541 ataagcctca tccctcgtac tcgccaagga tcgattcagg caatggttcg tcgaagttct
      601 tttcgatgac cactttccct ttggccgttc ccgccacccg ttcagcggtc ttgtcccgaa
      661 tgggaacaag gcgggctatt ggtgttcccg ccctcgctat agtgctcccc tctccgcttt
      721 ccacccggtt gagcaactct gataatctcg tcttggcttc gtgtatattc accctcatag
      781 acccataata gactaagtag tggactaatc actctctcat gaacaaggca ggatcgttcg
      841 ttgccctgcg agcgtcggcc tcctcaacct cttcggaatc gatgagaacc ctcaggctct
      901 ggttcaaggt ccagcctatc tgcccgcttc gctgctgctg acgcagcagg tcatatctgc
      961 cccgtaccag gagctcgcgg atggcagcat ccttggtgtc cagaaattcc gtggcaagga
     1021 ccaggcggcc gcgctggcgg tccgtgccgg ccgtcggtac caggacctgc gccattacgg
     1081 caaggagaac gtccgtcagt tcctgcacca tccactcctg tttctccacg gggaacatgg
     1141 acgctatccg cgaggggacc tgcgtggtca tgagcgtatg gatggttgtg aacacgaggt
     1201 gacccgtctt ggccgcttgg atcgccgccg acgctgtttc gggatctctc acctcaccta
     1261 ccaggatcac gtccggccgc tggcgcatcg ccgccctcag cgcccgttcg aaggacagtg
     1321 tcgtggtgcc cacctcgcgc tcgatgatgt tgcccatccc ttcagggatg tcgtattcaa
     1381 tggggtcttc tatggtgacg cagttgaggc accgtgtcgc cacaagatac gctatgaggg
     1441 aggcgagcgt tgtcgatttg ccgctgcctg tggggccggc aatgaagatg ataccccccg
     1501 tcggactctc aaggacaagt ctcaagaggt cgcccggaac gccgaggttg agcgggttcg
     1561 tttccacgga cttcaggcgc ctcatgacga tggaaacccc ccttttcgtc atgcacaggt
     1621 tgacgcgaaa cctgaaggtt ttcttctctt tgccgctgcc cacctgtctc gcataggaca
     1681 ggtccttctc ctccctgtcg tagagctgcg cccgggcgcc ggagaccctg aggatacgct
     1741 ctatcgcttc gtctatctcg tatccttcgg gaggatcggc ggcagcggcc atgcgcccgc
     1801 ttatcctcag gaagaccggg ctttccgatt ggatgtagat gtcggaggcg tcctcactga
     1861 tcgccctgtc gatgatatcg tcgaggaaat tgctcatcgt ctacctgcag cgcagccatt
     1921 cggcgccaag gaggtatgcg agatatcttc tcagaacgcc cgagcggctg cagcccctct
     1981 gcctgacctc ttcctttcgc tttccccgcg cgaagaaata gagggccaca gacccgcaca
     2041 tgcccgtcgt gatgaggaaa acaccaatct cttccgacac gtatgacaga attgaagcga
     2101 tgccgttgcc cagcatagcg gggcaaatgc agataatcag gtcttccagg gatatattga
     2161 gcctcggcac gataatggcg ctgtttatgt tctggtagtc atgacgtgtc ccgggttcca
     2221 tggtgatcta tcccgcgctt gccgtcatgg tcgccttgat aatcccgagg atcgccgagg
     2281 ttgacacgag gcagcctccg aaggcaacct tcttccacgc accctgggca ccctgggggt
     2341 tgttgtcctt gttcgccatc accacgaagc cccatatgat gagacccgtc ccagcgcaga
     2401 aagagatcga caggagaccg tacatgatta tattggccag attgctcagg aactccccgg
     2461 catcggtggt ctccgttgcc cacctgctct cgttgaatgt ctgcgcccat ccgtccccgg
     2521 tacgcacggc gccgaataac aggcacaatg cggcggaaag gaagaacctg cacaggctcc
     2581 ccaattttcc cgcccttatc ttc
//
LOCUS       DAOPOA010000318         2594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_43264_length_2594_cov_3.402521, whole genome shotgun sequence.
ACCESSION   DAOPOA010000318 DAOPOA010000000
VERSION     DAOPOA010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43264_length_2594_cov_3.402521"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PKJ17_07255"
     CDS             complement(<1..739)
                     /locus_tag="PKJ17_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOD75805.1"
                     /translation="MKDVPLDRQPVLKKRCMAAGAVLLLSIAVMALACLLPACSGKPG
                     DAGQPEMRTIVASDTILSEIITSLLPPDRYRVEAILPPGQCPGHYDVKPTDIEKMKKA
                     VVIVSFRGMPFMGKAGSDDERQLVIDPEDRNWMAPGSYVQGLELLAARLSERFPGDRA
                     EIARRKEHAIRLATQGADRLLQKIAGAGLSGRPVIASSMQKEPLEWMGFQVVAEYGRQ
                     EAMSARDVVRLTEAGKHRGAVMVVDNLQ"
     gene            complement(771..>2594)
                     /locus_tag="PKJ17_07260"
     CDS             complement(771..>2594)
                     /locus_tag="PKJ17_07260"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOD75806.1"
                     /translation="QSKNLVGSQTSHSLYIRGRGSSHPSSDIVVQFDGAPRYGGLFGQ
                     VLGDGIAVPTIGSIEIYKSPQPSRFGSGYAAVNILPKYMKKEGQEGILGFSGGSYGTL
                     DENVSAGLKKGPFDVYASQSYVTTDGDRANSAARQQSLYANTGYQVSKEWNIRFLINQ
                     VNASTEAPMPDMIPNTVNGVSWPGAERYKTDTLFATLTVSHEYERASGYLKGYWNDTT
                     FDLLQELTNGNQYANGTGGLWSRQKVILYGARAREALHLWPGGEILVGADLDWTNLKN
                     TQRTYSGLPPTGAGAAGVNGGKARRVWDFPDTRLLSPYIAASHTFGRPEAFHVTPSAG
                     LRYFNHDEFENKTASQAGLVVGYATTDLKIGYSRGINYPSPVVLMNFVRTDTPVANAG
                     QYWRKIKPEVVDHYEAGLSHSWPRMATVGATVFRDKGSDRFLAYMGGTIPTQFNDHIG
                     HYEIEGLELTGAVTPVKDLEIFSAATWLTTDATGANGIESNHIPYTPGFQFQAGAKWR
                     FLDNFQIFADIQHLRNLYQGTFSRGGTFNTPQLTAKDKLNDITLVNTRISYRFDHAPL
                     RLRDSDIFFAVNNIFNSDYEYAKGYPMPGTTFFGGFTLKFN"
BASE COUNT          443 a          748 c          836 g          567 t
ORIGIN      
        1 attggaggtt gtcgacgacc atgaccgccc cacggtgttt tccggcctca gtgagccgca
       61 cgacgtcccg cgcggacatc gcttcctgcc tgccgtattc cgcgacgacc tggaagccca
      121 tccattccag gggttccttc tgcatcgaag aggcgatgac gggtctgccg gagagcccgg
      181 cgccggcgat cttctgaagg agcctgtccg ctccttgtgt tgccagacgg atggcgtgtt
      241 ctttccggcg ggctatctcg gccctgtccc cgggaaaacg ctccgaaagc ctcgcggcaa
      301 ggagctcgag gccctgcacg taggagccgg gagccatcca gttgcggtct tccggatcga
      361 tgacgagctg acgttcgtca tccgacccgg ctttgcccat gaagggcatg ccccggaagg
      421 aaacaatgac gaccgccttc ttcattttct cgatgtccgt cggtttcacg tcatagtggc
      481 cgggacactg ccccggggga agtatggctt caacgcgata gcggtccggc gggaggagcg
      541 atgtgatgat ctccgagagg atagtgtccg atgctacgat ggtgcgcatc tctggctgcc
      601 cggcatcacc gggtttgccg gagcacgcag ggagaaggca ggcgagggcc atcaccgcga
      661 tcgaaaggag caggacggcc cctgccgcca tgcagcgttt cttgagaacc ggttgtcgat
      721 ccagtggaac gtctttcacg tgttcctcgt gcccctcgcg gcaggcgggc ctagttgaac
      781 ttcagtgtaa acccgccgaa gaaggtcgtc cccggcatgg ggtagccctt tgcgtattca
      841 taatcgctgt tgaagatatt gttgacggcg aagaagatgt ccgaatccct gagcctgaga
      901 ggcgcatggt cgaaacgata gcttatccgg gtgttgacga gtgtaatgtc gttcagcttg
      961 tcctttgccg taagctgcgg ggtattgaac gtgccgcccc gggagaacgt tccctggtaa
     1021 aggttccgga ggtgctgtat gtcggcgaat atctgaaagt tgtcgagaaa tctccacttt
     1081 gcgccggcct ggaattggaa gcccggtgtg taaggaatgt ggttgctttc aatgccgttg
     1141 gcccccgtgg cgtctgttgt gagccaggtg gcggcagaga agatctcgag gtcctttacg
     1201 ggggtcaccg cgccggtcag ctccagtcct tcgatctcgt agtgcccgat gtgatcgttg
     1261 aactgggtcg ggatggtccc gcccatgtac gccaggaagc ggtcgcttcc cttgtcgcga
     1321 aagaccgtcg cgcccaccgt ggccatcctc ggccaggaat ggctgaggcc cgcttcgtag
     1381 tggtcgacca cctcgggctt gatcttcctc cagtactgcc cggcattggc aacgggtgta
     1441 tcggtgcgga cgaagttcat gagaacgacg ggggaaggat agttgatgcc ccgggaataa
     1501 ccgatcttca ggtcggtggt ggcgtatcca accaccagac ctgcctggga agcggtcttg
     1561 ttctcgaact cgtcgtggtt gaaatatctg agccccgccg acggcgtgac gtgaaaggct
     1621 tccggccggc cgaacgtgtg gcttgcggcg atgtagggtg acaggagccg ggtgtcggga
     1681 aaatcccaga cccgtctcgc cttgccgccg ttgacgccgg ccgcgcccgc gccggtaggg
     1741 ggcagtccgc tgtatgtccg ctgtgtgttc ttgaggttcg tccagtcgag gtcggcaccg
     1801 acaaggatct cgccgcccgg ccacagatgc agtgcttctc tggcccgggc gccatagagg
     1861 atgaccttct gccgggacca gaggccgccg gtaccattgg cgtactggtt gccgttcgtc
     1921 agctcctgaa gcaggtcgaa ggtggtgtcg ttccagtagc ccttgagata gccactcgcg
     1981 cgctcgtact cgtggctcac agtcagcgtg gcgaaaaggg tgtccgtctt gtagcgttcc
     2041 gctccgggcc agctgacccc gttcaccgtg ttcggtatca tatccggcat gggggcctct
     2101 gtcgaggcgt tcacctggtt gatgaggaac ctgatattcc attctttact gacctggtaa
     2161 ccggtattgg catagaggct ctgctgacgg gcggcggaat tggcacggtc cccgtcggtg
     2221 gttacgtaac tttgggacgc gtagacgtcg aagggtcctt tcttcagacc cgccgagaca
     2281 ttttcgtcca gggtgccata gctgcccccg ctgaaaccga ggattccctc ctgtccctct
     2341 ttcttcatgt atttcggcag gatgttgacg gccgcgtagc cgctgccgaa ccgggagggc
     2401 tgggggctct tgtatatctc tatgcttccg atcgtgggaa cggcgatgcc gtcgcccagg
     2461 acctgtccga agagcccacc atagcgggga gcaccgtcaa actgaacgac gatgtcggaa
     2521 ctggggtggc tcgacccgcg gccgcgtatg tagaggctgt ggctcgtctg gctccctacg
     2581 aggttcttgc tctg
//
LOCUS       DAOPOA010000319         2594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_43266_length_2594_cov_3.282001, whole genome shotgun sequence.
ACCESSION   DAOPOA010000319 DAOPOA010000000
VERSION     DAOPOA010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43266_length_2594_cov_3.282001"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKJ17_07265"
     CDS             complement(<1..310)
                     /locus_tag="PKJ17_07265"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD75807.1"
                     /translation="MPRFREKAGDVGLKGLIELSFSDWEGRMSSVLFTGGCNFRCPYC
                     HNRDLALGGAGIDDMPYDLVKERLDAQKLWVDSVVITGGEPTLHRGMTAVMADLKSSG
                     Y"
     gene            complement(294..593)
                     /locus_tag="PKJ17_07270"
     CDS             complement(294..593)
                     /locus_tag="PKJ17_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75808.1"
                     /translation="MDTVKIFVKNDCPRCPVAKKLYDELASRLGSSGVTVEKWSVDET
                     EGLVEAAMHMVLTTPTVLVFDGEENKVADWRTVVPSPDEVIEALNIAGGEHAPVP"
     gene            complement(568..960)
                     /locus_tag="PKJ17_07275"
     CDS             complement(568..960)
                     /locus_tag="PKJ17_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75809.1"
                     /translation="MARKKEKKLLLDNVIQADFTQTRYDYKLQSPTYILLLARDSRGN
                     PGTGRAIQVTPGFNFSTFLEMGSKGYLTGNAKKDYQEYMKGVDAGRADITAVFRDSGI
                     GSALTYEKKSTTPEEDEEEDRGYGQDFC"
     gene            complement(980..1594)
                     /locus_tag="PKJ17_07280"
     CDS             complement(980..1594)
                     /locus_tag="PKJ17_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75810.1"
                     /translation="MMKAGKDKPAERTPAPDRGAMDIMSVIKQLGDLRNVKKDVANHA
                     GDNAYFIRAFHRCFELGPFVVATPSDNMAGSFLSSTETGVLVIRKTSASTKHALVLKR
                     FVVTEDSPKESPERVKPGDAVIWKTYFRHMDYPVTHGESNMTVPEIVSSWDKFGPIFA
                     DLLETELNNVDTVMKAIRELEGHLAPDMMTEYATGGRYTPSTGR"
     gene            complement(1591..2181)
                     /locus_tag="PKJ17_07285"
     CDS             complement(1591..2181)
                     /locus_tag="PKJ17_07285"
                     /inference="COORDINATES: protein motif:HMM:NF020125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75811.1"
                     /translation="MQIASAYGKNAAARIEFRDGKVWLTIAPAAQGNNGRKYEWSQKK
                     NFNLDRSELHAMKVVMDTALRRDVASAQVLCNSLFGKNRNNCLFTHVTNQGQVLGGLS
                     LHTDDKGGIYAPFSFRISYYSKESAGKEDSVQVSMTRINAMMIIRDIEVFNTEYVQSM
                     VRLEMADTQAKERQPGSPRGEENNERGFDQFPGDLP"
     gene            complement(2192..2407)
                     /locus_tag="PKJ17_07290"
     CDS             complement(2192..2407)
                     /locus_tag="PKJ17_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75812.1"
                     /translation="MSLIRVFGGRPYKVAIKDQAGAEKPVMDYLFASSPEDARDQAVE
                     AFPDVGADMFVVSEVAEEPRQCTPSCG"
BASE COUNT          478 a          782 c          688 g          646 t
ORIGIN      
        1 tgtagcccga tgacttgaga tcagccatga cggctgtcat cccccgatgg agcgttggct
       61 cgccgccggt gatgacgacg ctgtcgaccc agagcttctg agcgtcgagg cgctccttca
      121 ccaggtcata cggcatatca tcgatgccgg cgcccccgag ggcaaggtcg cgattgtggc
      181 agtaggggca cctgaaatta catccacccg taaaaagaac ggaggacatg cgcccttccc
      241 agtcgctgaa agacagttca atgaggcctt taagcccgac gtctccagct ttttcacgga
      301 accggggcat gctcccctcc ggcaatattg agggcctcga tgacctcatc aggcgacggt
      361 acaacggtcc tccagtcggc gaccttgttc tcttctccgt cgaacacgag cacggtcggg
      421 gtggtcagga ccatgtgcat ggcggcctcc accaaccctt ctgtctcatc cacactccac
      481 ttttccaccg tcaccccaga cgaacccaac cgggaagcca gctcatcata gagtttcttt
      541 gccacaggac acctggggca atcattctta acaaaaatct tgaccgtatc cacgatcttc
      601 ctcctcgtct tcctccggtg ttgtgctttt tttctcatat gtcagcgccg aaccaatccc
      661 cgaatcacgg aacactgccg taatatcggc ccggccggcg tcaaccccct tcatgtactc
      721 ctggtaatcc ttcttcgcgt tacccgtgag atagcccttg cttcccatct cgaggaatgt
      781 cgagaaattg aagccgggag ttacctggat ggccctgccg gtgcccgggt tcccgcggga
      841 atccctggcg agcaggagga tgtaggtggg agactgcagt ttgtaatcat atctcgtttg
      901 agtgaagtcc gcctggatca cgttgtcgag cagcagcttc ttctcttttt ttctggccat
      961 gtcctctccg cgcccccggt caccgccccg ttgagggcgt atagcggccg ccggtggcgt
     1021 attccgtcat catgtcgggt gcaagatgac cttccaattc cctgatggcc ttcattaccg
     1081 tgtcgacgtt attcagttcg gtctcgagaa gatccgcgaa tatgggaccg aacttgtccc
     1141 atgaagagac gatctcgggg acggtcatgt tgctttcacc gtgtgtcacg ggatagtcca
     1201 tatgacggaa atatgttttc cagatcacgg catccccggg ctttaccctc tccggggatt
     1261 ctttggggct gtcctccgtg accacgaacc tcttgaggac cagggcgtgt tttgtactgg
     1321 cggacgtctt ccggatgacc agcacccctg tttccgtcga ggagagaaaa ctcccagcca
     1381 tgttgtcgga gggagtggcg accacgaaag gacccagttc aaaacagcga tggaaggccc
     1441 ggataaaata agcattgtcc ccggcgtggt ttgccacgtc cttcttgaca ttgcggaggt
     1501 caccgagctg cttgatgacg gacatgatgt ccatggcgcc gcgatcaggg gcgggggttc
     1561 tttccgcagg tttgtccttt ccggccttca tcatggcagg tctccgggaa attggtcgaa
     1621 gccacgttca ttattttcct cgccgcgcgg ggaccctggc tgccgctctt tcgcctgtgt
     1681 gtcggccatt tccagcctga ccatgctctg gacatactcg gtattgaaaa cctcgatgtc
     1741 tctgatgatc atcatcgcat tgatcctggt catgcttacc tggacggagt cctcttttcc
     1801 cgcggactcc ttgctgtagt aggagatccg gaatgaaaac ggagcgtata tcccgccctt
     1861 gtcgtccgtg tgcagactca gcccgccaag aacctgtccc tggttcgtaa cgtgcgtgaa
     1921 aaggcagttg ttcctgttct tgccgaaaag ggagttacac aggacctgcg cggaggcgac
     1981 atcgcgtctc agggccgtgt ccatgacgac tttcatcgcg tggagttcgc tcctgtccag
     2041 gttgaagttc ttcttctggc tccattcgta tttcctgccg ttgtttccct gggcggcggg
     2101 ggctatggtc agccagacct tcccatcccg gaattctatc ctcgccgccg cgttctttcc
     2161 gtatgcgctc gctatttgca taatgcctcc cttagccaca tgacggtgtg cactgccggg
     2221 gttcttccgc cacctcggat acgacgaaca tatccgcccc gacatcaggg aaggcttcca
     2281 ccgcctggtc cctggcatct tccgggctcg aagcgaaaag atagtccatc acgggctttt
     2341 ccgcaccggc ctggtccttt atggcaacct tatacgggcg tccaccgaac acgcgtataa
     2401 ggctcatttt ctcattcttt gaaactgtac gttctgacac ttgtttctcc tttcctagaa
     2461 atgctttttc ccgggggacg gggcggcgat acccgtattc tgcgccacgt cggtggtctg
     2521 ttgctgtgca atgtcgacgg cctggtacct ttcctggtac atcgcaggga gatctatcgg
     2581 cggtttcacg ttcc
//
LOCUS       DAOPOA010000320         2590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_43375_length_2590_cov_2.430375, whole genome shotgun sequence.
ACCESSION   DAOPOA010000320 DAOPOA010000000
VERSION     DAOPOA010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43375_length_2590_cov_2.430375"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PKJ17_07295"
     CDS             <1..553
                     /locus_tag="PKJ17_07295"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOD75813.1"
                     /translation="RFFLLALLVFCFASFCLYFFRNPRRRTSAGAGELVSPADGTVVD
                     ISDVGEEEFLGGTATRIAIFMSPVDVHVNRAPCSGLVVAMKHVAGKFAMAFGKDIEEQ
                     NERNFILFENEGERTLIVQIAGFLARRIIPYVKVDERVEQGQEVGIIAFGSRVDIYFH
                     KGYVPVVDLHAKVKAGITVLARK"
     gene            563..1351
                     /gene="pssA"
                     /locus_tag="PKJ17_07300"
     CDS             563..1351
                     /gene="pssA"
                     /locus_tag="PKJ17_07300"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOD75814.1"
                     /translation="MNYAKKKKCQPMKGMYLLPNFLTSLSLLSGFYAIIATMDRRFTY
                     AAIAIFISAIFDMLDGRVARMTNSSSRFGVEYDSLSDVIAFGVAPAILAYTWALKSYG
                     KFGWLAAFLFVACGALRLARFNIQVDTVQKKHFLGLPIPAGAATIAGIVLFFSWLGYK
                     GGLKTVIMPIVIYCLALLMVSKFRYISFKDMGFVRARPFMATVGVIMLLVIVLTEPYL
                     TLFVATSAYAVSGPVYTLILHFRKKPSEELSRPRETQAGQGGRQ"
     gene            complement(1305..1994)
                     /locus_tag="PKJ17_07305"
     CDS             complement(1305..1994)
                     /locus_tag="PKJ17_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOD75815.1"
                     /translation="MDQQKKWDVDCVIYDCDGVLFDSLDANRRLYNSIALAARGTGLN
                     DDELCYCHTHTVFQSIAHICRNDAQSERRAREFFASTIDFRDFIPYLTMEPNVTETLE
                     RLKARGISRAISTNRTTSMKHIMERYGLWQYFDIVVTAAHQAEAGSDGGPARTILQAK
                     SKPDPEGVEMILQALKVKREATLYVGDSEVDMGTARSSGVRFVAYKNGALEADGSIDD
                     HLALLEFLSDG"
     gene            2164..>2590
                     /locus_tag="PKJ17_07310"
     CDS             2164..>2590
                     /locus_tag="PKJ17_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOD75816.1"
                     /translation="MISVSVPGWGDLDIEYLVIDYNGTCAFDGKMKESVKEMLERVSR
                     YIKVFIITSDTYGNIDSEGNTIGFSIIKVGKESSAQEKAKIIKELGPEKIVAIGNGSN
                     DVLMLKEASLGIGVIGEEGCSKDVLREADFFVKDINDALS"
BASE COUNT          563 a          718 c          720 g          589 t
ORIGIN      
        1 caggttcttc ctcctggcgc ttcttgtctt ttgtttcgca tcattctgtc tgtatttttt
       61 ccggaacccc aggcggcgga ccagtgcggg ggcgggtgaa cttgtctccc ccgcggacgg
      121 gaccgtggtc gacataagcg atgtcggtga ggaggagttc ctcggcggca ccgccacgag
      181 gatagcgatc ttcatgtcgc ccgtagatgt ccatgtcaat cgggctccct gttccggtct
      241 tgtcgtcgcc atgaaacacg ttgccggcaa gttcgccatg gccttcggca aggatatcga
      301 ggagcagaac gaaaggaact tcattctctt cgagaacgag ggcgaaagga ccctgatcgt
      361 ccagatagcg ggctttcttg cccgccgcat cataccctac gtgaaggtcg atgagcgggt
      421 ggaacagggt caggaagtgg gcatcatagc cttcggctcc cgggtggaca tttattttca
      481 caaagggtac gtacctgtgg tagacttaca cgcaaaggtc aaggccggca taacggtcct
      541 ggcgcgcaag taaggaggtg ccatgaacta cgcgaaaaag aagaaatgcc aacccatgaa
      601 ggggatgtac cttctcccca attttctcac ttccttgagc cttctctccg gtttctacgc
      661 catcatagcg accatggaca ggcggttcac atacgccgcc atcgccatat tcatatccgc
      721 catctttgac atgctcgacg gccgggtggc acggatgacc aattccagca gccgcttcgg
      781 cgtggaatac gattccctct ccgatgtcat cgccttcggc gtcgcccccg cgatcctcgc
      841 ctacacctgg gcgctcaaga gctacggcaa gttcggctgg ctcgccgcct tcctcttcgt
      901 ggcctgcggc gcgctgagac tggcgaggtt caacatccag gtcgacacgg tgcagaagaa
      961 gcactttctg ggtcttccca tacccgcggg tgccgcgacc atcgccggta tcgtcctctt
     1021 tttctcatgg cttggctaca agggcgggct gaagaccgtt atcatgccca tcgtcattta
     1081 ctgtcttgcc cttctcatgg tcagcaagtt ccgctacatt agcttcaagg atatgggatt
     1141 tgtcagggca cggcccttca tggcaacggt gggggtcatc atgcttctcg tcatcgtcct
     1201 caccgagcca tacctgacgc tttttgtggc cacgtcggcc tacgcggtgt cgggacccgt
     1261 ctataccctt atcctgcact tcaggaagaa accgtcggaa gagctcagcc gtccgagaga
     1321 aactcaagca gggcaaggtg gtcgtcaata gaaccatccg cctcgagggc accgttcttg
     1381 taagccacga acctgacccc cgaggaccgc gccgttccca tatcgacctc ggaatcgccg
     1441 acgtacaggg tggcctcgcg cttcaccttc aatgcctgaa ggatcatctc gaccccttcg
     1501 gggtccggct ttgacttcgc ctggagtatc gtccgcgcgg gtcccccatc gctcccggct
     1561 tcagcctgat gggcagcggt caccacgatg tcgaaatact gccataaccc gtaccgttcc
     1621 atgatatgct tcatcgacgt cgtccggttc gtgcttatcg cccgcgatat gccccgtgcc
     1681 ttcagtctct ccagggtctc cgtgacgttg ggctccatcg tgaggtaagg gatgaaatcc
     1741 cggaagtcta tggtgctcgc gaagaattcc cgcgcacggc gttcggactg agcgtcgttg
     1801 cggcagatgt gggcgatgga ttgaaagacg gtgtgggtgt ggcagtagca aagctcgtcg
     1861 tcattgagcc cggtaccgcg cgcggcaagg gcaatgctgt tgtagagacg cctgttggcg
     1921 tcgagggagt cgaaaagcac tccgtcgcag tcgtagatga cgcaatcgac atcccatttc
     1981 ttctgctggt ccatggtccc attctcctcg tactacaatt tcgaaccttc tgcttttaaa
     2041 ctgtaagttg caaaaaatca agagaaaacc tgtgaagaat atcgtttaat gacgcaaatt
     2101 aatggttcta attgcattaa tatatgctac aattgtatcc gaaagactac caaggaggct
     2161 actatgataa gtgtttccgt tcccggatgg ggcgatcttg atattgaata cctggtcatc
     2221 gactacaacg ggacctgtgc ttttgacgga aagatgaagg aaagcgtcaa ggagatgctc
     2281 gaaagggtgt cccgctacat aaaggtgttc atcatcacgt ccgataccta cggcaacata
     2341 gacagcgaag gaaacacgat cggtttcagc atcatcaagg tggggaagga atcgagcgcc
     2401 caggaaaagg cgaagataat aaaggagctg gggcccgaga agatagtggc cataggcaac
     2461 ggctcgaacg acgtattgat gctcaaggag gcttccctcg gaatcggcgt gatcggcgag
     2521 gaaggatgct ccaaggatgt gctgagggag gccgatttct tcgtaaagga cataaacgac
     2581 gcactgagta
//
LOCUS       DAOPOA010000321         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_44044_length_2559_cov_3.784744, whole genome shotgun sequence.
ACCESSION   DAOPOA010000321 DAOPOA010000000
VERSION     DAOPOA010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44044_length_2559_cov_3.784744"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PKJ17_07315"
     CDS             complement(<1..555)
                     /locus_tag="PKJ17_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOD75817.1"
                     /translation="MEPTNELYAVRKEKEKSLKEQGIDTYPQDRGPYTTTKDVEERFG
                     PQSAEDLEKNEERVAIAGRIMAFRDFGKSAFIHVQDRKGKMQAYIRKDVLKSPGYDVF
                     KKFDICDIIGLEGKVFKTRTGELTVLADSVKLLTKSLRPLPEKWHGLKDVEERYRKRY
                     LDLIVNEKVRDVFKTRARIIDYIRR"
     gene            complement(585..1850)
                     /gene="rimO"
                     /locus_tag="PKJ17_07320"
     CDS             complement(585..1850)
                     /gene="rimO"
                     /locus_tag="PKJ17_07320"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOD75818.1"
                     /translation="MRFRIVSLGCPKNLVESEYMVGRLCSAGHTVSDDGDTIIVNTCA
                     FIAEAARESIETILEAAREPGRKVVVTGCLVERYGEELAGLLPEVDAFVGRSRYGEIE
                     RVIGKSGLHLGKGGFSETFPRQVLTKPPTAYLKIQEGCDNRCRYCTIPAIRGGLTSRA
                     EKDVIGEFRWLLDQGYREINVIGQDITSYGKDAGREQGLTGLIRSLLKEKGDWHLRLL
                     YMHPKGITEGLIDVMGSDARVIPYLDVPIQHSEDRILSLMGRGHTRAYLEETLAMMRQ
                     RIPGAVLRTSIIVGFPTETEEEFEALLSFVRRHQFDMLGAFMYSREEGTAAYRMKGQI
                     RKGVKRDRYTRLMETQVEISRTRLADLLGKTVKVIVEDVGASPKVGRMLTQAPDIDGV
                     AFVNGACSEGDIRDGKVVKTLDYDVVVEV"
     gene            complement(1840..>2559)
                     /locus_tag="PKJ17_07325"
     CDS             complement(1840..>2559)
                     /locus_tag="PKJ17_07325"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOD75819.1"
                     /translation="DPVSFVHRWHLDADREIAGFIAAQFAYGRIEVFMGFLEKLFALM
                     DEGPERFVRRGAWEGLKGLYYRFQKEDDLVHLFEVLHRIVGDHGSLGAMFRSFYEGDT
                     RRAVWRIRDAYLAGDDRLTFFFPRPSSTGAQKRWSMFLRWMVRKDEIDIGIWDFIDPA
                     DLTIPLDTHIYKVGRCLGWTGRKTPSYRAAREITEALKRYCPSDPLKYDFFLCHGVGI
                     GAGCTGKRSDRCREKCAVHEI"
BASE COUNT          452 a          817 c          723 g          567 t
ORIGIN      
        1 ccgcctgata tagtcgatga tccttgcccg cgttttgaaa acgtcccgca ccttttcgtt
       61 gacgatgagg tcgaggtagc gcttgcggta gcgttcctcg acgtccttga gcccatgcca
      121 tttctcgggc agagggcgca gggacttggt aaggagcttt acgctgtcgg cgagcaccgt
      181 cagttcccct gttctcgtct tgaagacctt gccctcgagg ccgatgatgt cgcagatgtc
      241 gaacttcttg aatacatcgt agccggggct tttcaggaca tccttgcgga tataggcctg
      301 catcttcccc ttcctgtcct gcacatggat gaaggcgctc ttgccgaaat cgcggaaggc
      361 catgatgcgt cccgcgattg cgacacgctc ttcgttcttt tccaggtctt cggcgctttg
      421 cggcccgaac ctttcttcca cgtccttcgt cgtggtatag ggacccctgt cctgggggta
      481 ggtatcgatg ccctgctcct tcaggctttt ttctttttcc ttgcgcacgg catagagctc
      541 atttgtcggt tccatcgtat cctctgtcaa ttggcgatat atttctatac ttccacaacg
      601 acgtcataat caagtgtttt cacgaccttt ccgtcgcgta tgtctccttc ggaacaggcg
      661 ccgttcacga aggcgacccc gtcgatgtcg ggcgcctggg tgagcatcct gcccaccttc
      721 gggctcgcgc cgacgtcctc cacgatgacc ttcaccgtct tccccagaag gtccgcgaga
      781 cgggtgcggg atatttccac ctgcgtctcc atgaggcgcg tgtagcggtc gcgctttacc
      841 cctttgcgta tctgcccctt catcctgtac gccgcggtgc cttcctccct ggagtacatg
      901 aaggcgccca gcatgtcgaa ctgatggcgg cgcacgaagg agagaagggc ctcgaactcc
      961 tcttccgtct cagtcgggaa cccgacgatg atggacgtcc ggagcaccgc cccgggtatg
     1021 cgctgcctca tcatcgcgag cgtctcttcg aggtaggccc tggtatgccc cctgcccatg
     1081 agggagagga tacggtcctc tgaatgctgg atggggacat cgaggtaggg tatcacccgc
     1141 gcgtcgcttc ccatgacgtc gatgagcccc tccgtgatcc ccttcggatg catgtacagg
     1201 agcctgagat gccagtcacc cttttccttc aggagtgagc ggataagacc ggtcagcccc
     1261 tgttcccgtc cggcgtcctt tccgtaggac gtgatgtcct gaccgatgac gttgatctca
     1321 cgataaccct ggtcgaggag ccagcggaac tccccgatca cgtccttttc ggccctcgac
     1381 gtcaagcctc cgcggatggc cggtatggtg cagtaccggc accggttgtc gcagccttcc
     1441 tgtattttga gatacgccgt cggcggcttc gtaagcacct gccggggaaa ggtttccgaa
     1501 aaacctcctt tcccgaggtg caggccgctt ttgccgatga cgcgttctat ctcaccatac
     1561 cgtgacctgc ccacgaaggc atccacctcg ggaaggagcc cggcaagctc ttcgccgtag
     1621 cgctccacga gacatcccgt gacaacaacc ttcctgcccg gctcccgggc cgcctcgagg
     1681 atggtctcga tggactcccg cgcggcctcg gcgatgaagg cgcaggtgtt gacgatgatc
     1741 gtgtcgccgt catcggagac ggtgtgcccc gccgagcaga gtctgcccac catgtattcc
     1801 gattcgacga ggttcttggg acatcccagg cttacgatcc taaatctcat gaacggcaca
     1861 tttctccctg catctgtcgc ttcttttccc cgtgcacccc gccccgatgc cgacgccgtg
     1921 gcaaaggaaa aagtcgtatt tcaaggggtc cgatgggcaa tagcgcttca aggcctccgt
     1981 gatctcccgg gcagcccggt acgaaggggt cttccgcccg gtccagccga ggcatcttcc
     2041 caccttgtat atgtgcgtgt cgaggggtat ggtaaggtcc gccggatcga tgaaatccca
     2101 gatgccgata tctatctcgt cctttctcac catccaccgg aggaacatgc tccagcgctt
     2161 ctgcgccccc gtcgacgacg gcctcggaaa gaagaaggtg agcctgtcgt caccggcaag
     2221 gtaggcatca cggatgcgcc agacggcccg ccgggtgtcg ccctcgtaaa aggagcggaa
     2281 catggcgccc agggacccgt gatcgccgac gatccggtgc agcacctcga aaaggtgaac
     2341 aagatcgtcc tccttctgga agcggtagta caggcctttc agtccctccc acgcgccacg
     2401 ccggacgaag cgttcggggc cctcgtccat cagcgcgaag agcttctcga ggaagcccat
     2461 gaagacctcg atcctcccgt aggcgaactg cgccgcgatg aagccggcga tctccctgtc
     2521 ggcgtcaagg tgccagcggt ggacgaagga aacggggtc
//
LOCUS       DAOPOA010000322         2558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_44069_length_2558_cov_3.779864, whole genome shotgun sequence.
ACCESSION   DAOPOA010000322 DAOPOA010000000
VERSION     DAOPOA010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44069_length_2558_cov_3.779864"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PKJ17_07330"
     CDS             <1..1042
                     /locus_tag="PKJ17_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75820.1"
                     /translation="TDFLLERKDEWKGTLIIPTDDYGVKFLAANRDRLSAHYAIPAPD
                     LDVVNTIVDKKSLYAVARRIGVPVPAIYSPRTMDELRSLEGVLRFPCLVKPGLAHLFI
                     REFGLKMFEVHSFVDLVRHYRELTDDFTSDRFELMICDIIPGPDSRQMVQYVSYMDRS
                     GEVLASMTSRKLRQDPPRYGQGRVAISQRVDGVDGISLRLLRELGYHGFSEIEWKYDP
                     RDGTYKLIEINPRFIFYTGLCVACGINFPYIQYADLVLGRKIRIGSFKENVYWIHEYA
                     DALHTVLNHRLEDLSLRDYVRPYLGKKCFAIFDPRDCRPFFEQWKQHAGNVIRRWRNA
                     RTSPFRINGVSP"
     gene            1080..>2558
                     /gene="asnB"
                     /locus_tag="PKJ17_07335"
     CDS             1080..>2558
                     /gene="asnB"
                     /locus_tag="PKJ17_07335"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOD75821.1"
                     /translation="MCGITGVLNLNGGRPPEPSRLVRANDALSHRGPDDSGTFTDGQV
                     GLAMRRLSIIDVARGHQPLSNENGAISIVFNGEIYNHLELREELVRRGHVFRTRSDTE
                     TIVHAYEQWGMEGCLSRLRGMFAFAIWDSSRRALFLCRDRMGIKPLYYAEEGGRLYFS
                     SEIRGILLQSGIPCRVDMAAVDAFLTVGFVPNPRTICKGIRKLPPAHFLTVRNGTVTM
                     RKYWELAYSEGNGSTVRELTEEFQDLLAECIGMHLMSEAPLGALLSGGIDSATIAAFA
                     QRSLSTRLKTISISFDSCRLNEADAARETAGALGTDHVPVAFGKDSMDDYPEALYYLE
                     EPLAEPVFVTEYNMHRAVRQKGLKVVLTGEGSDELLAGYHWHRGEVWSRPLLGLPFFL
                     RAFVAEGLLLRSRGAGAMGMYRVLREGRSEAQDQYRAWLTIGDPKIKERLLAREVSAA
                     LKGNGGSLLDSWKDHLPAVAGRPLSDQMLWLQSRTRMVDDINH"
BASE COUNT          523 a          748 c          801 g          486 t
ORIGIN      
        1 gaccgacttc ctcctcgaga ggaaggatga atggaaggga acgctcataa tccccaccga
       61 cgactatggg gtcaaattcc tcgcggccaa cagggacagg ctgtccgctc actacgctat
      121 ccccgcgccc gatctcgatg tcgtaaacac cattgtggac aagaagtccc tctatgccgt
      181 cgcccggcgc atcggtgtcc cggtgcccgc catttactcc ccccgaacga tggacgagct
      241 ccggtccctg gaaggggttc tcaggttccc ctgcctggta aaaccgggtc ttgcgcatct
      301 cttcatacgg gaattcggcc tcaaaatgtt cgaggtccac agtttcgtcg atctggtgag
      361 gcactacagg gaactcaccg acgacttcac gtccgacagg ttcgaactga tgatatgcga
      421 tatcattccc ggcccggaca gcaggcagat ggtccagtac gtttcttaca tggacaggtc
      481 gggagaggtg cttgcctcca tgacgtcgag gaagctcagg caggacccgc cgcggtacgg
      541 gcaggggagg gtggcgataa gccagagggt cgacggcgtg gatgggataa gcctgaggct
      601 tctcagggaa ctcggctacc acgggttttc cgagatagag tggaaatatg acccccgcga
      661 cgggacgtac aagctcatag agatcaatcc ccggttcata ttctacacgg gactctgcgt
      721 cgcctgcggt atcaactttc cgtacatcca gtacgcggac cttgttctcg gcaggaagat
      781 caggatcggc tccttcaaag agaacgtcta ttggatccac gaatacgcgg acgcactcca
      841 taccgtgctg aaccatcgct tggaagacct ttcgctcagg gactacgtga ggccctacct
      901 ggggaagaag tgtttcgcca tattcgatcc gagggactgc aggcccttct tcgaacagtg
      961 gaaacaacac gctggcaatg tgatcaggag gtggaggaac gcgaggacct ccccgttcag
     1021 aataaacggt gtttcaccat gataaacgtt cacggtgtca tcgcctggaa ggaggcagta
     1081 tgtgtggcat taccggtgtt ctgaacctta acggcgggag gccgccggag ccgtcccggc
     1141 tcgtaagggc caacgacgcg ctctcgcaca gggggcccga tgattcgggg accttcaccg
     1201 acgggcaggt ggggcttgcg atgcgtcgcc tgagcatcat agacgtcgcg aggggccacc
     1261 aaccgttgtc caatgagaat ggagccatat ccatcgtctt caatggcgag atatacaacc
     1321 atctggagtt gagagaagag ctcgtaaggc gagggcacgt tttcaggacc aggtcggaca
     1381 cggagacgat cgtccatgcc tacgaacagt ggggcatgga aggctgcctg tcgagattgc
     1441 gggggatgtt cgcgttcgcg atctgggaca gctccaggcg cgcccttttc ctgtgcaggg
     1501 accgcatggg gatcaagccc ctgtactacg cggaagaggg gggcaggctc tacttttcca
     1561 gcgagatccg cggtatcctg ctgcaatcgg ggataccctg ccgcgtggac atggccgcgg
     1621 tcgatgcctt tctcaccgtc gggttcgtgc cgaacccgcg caccatctgc aagggaataa
     1681 ggaagcttcc ccccgcccac ttccttaccg tgaggaacgg gacagtcacg atgaggaaat
     1741 actgggagct cgcctacagc gagggcaacg gcagcaccgt tcgggagctg accgaagagt
     1801 tccaggacct tctggcggaa tgcatcggga tgcaccttat gagcgaggcg cctctcgggg
     1861 cgctcctgtc gggcgggatc gattcggcga cgatagccgc cttcgcgcag aggtctcttt
     1921 cgacacgctt gaagaccata tccataagct tcgacagctg ccgtctcaac gaggccgatg
     1981 ccgcccggga aacggccggt gctctcggaa cggaccacgt tcccgtcgct ttcggcaagg
     2041 acagcatgga cgattacccg gaggccctct actatctgga ggaacccctg gcggaaccgg
     2101 tgttcgtcac cgaatacaac atgcacaggg cggtgcggca aaagggcctc aaggtcgtgc
     2161 tcaccggcga ggggtccgac gagttgctgg ccggttacca ctggcaccgg ggcgaggtct
     2221 ggtcaagacc tcttctggga ctgcccttct tcctgcgcgc cttcgtggcc gagggtcttc
     2281 tcctgcgttc ccgcggtgcc ggcgccatgg ggatgtacag ggtcctgcgg gaggggcgca
     2341 gtgaggcgca ggaccagtac cgcgcgtggc tcaccatcgg cgacccgaag atcaaggagc
     2401 gtctgcttgc ccgcgaggtc tccgcggcgt taaaaggcaa tggaggatcg ctgctcgatt
     2461 cctggaagga ccaccttccg gccgtggcgg ggcggccctt gagcgaccag atgctctggc
     2521 tgcagtcgcg gacgcgcatg gtcgacgaca tcaaccac
//
LOCUS       DAOPOA010000323         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_44237_length_2551_cov_3.118189, whole genome shotgun sequence.
ACCESSION   DAOPOA010000323 DAOPOA010000000
VERSION     DAOPOA010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44237_length_2551_cov_3.118189"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2551
                     /locus_tag="PKJ17_07340"
     CDS             <1..>2551
                     /locus_tag="PKJ17_07340"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOD75822.1"
                     /translation="DDARDACLYITGRAGSFVSFLVAQRKRRTLVLYEDDDSAELVRD
                     EIAYYGNRDVVLFPPYQDRVFEEEDDLKRTGFLYRLAKDDNFVGLFPVSALTHTMPPP
                     DRISEDITTLKVGDAVFPEDIHDNLDRKGYELAPLVREAGQYAKRGSIIDFFPPASDR
                     PVRCEFIGDQILSLRHFNPLSQRSDKELARCDITSIIKKDGGGSSTIIDHLGGPLTLV
                     HPGDSLIDHALHAADAATKENWARLLAEAPQIDLSGVVGPSETQKTLRAVSNDDLRQL
                     FLLHKTRIFHILREKLQGEWSHYPYVYLFANTAHQAKRLQEIFESYGLVLPILPRISY
                     RRRSREWGIIEGPIRRGFRTDDIIVLTEDDIAGPKKRVVKRHWGSRDEFLASFKDLTE
                     GELVVHVDHGIGVYRGIVPLTVNGFTKDFLLIEYLGGDKLYVPVDDLHLVQKFIGSEK
                     FKPKIDRLGSRFWLNTKKRVRRQIEDIAGELLEIYAARQMAEGHSYPPEDELFREMES
                     RFEYEETEGQINAINDVLADLRSTRPMDRLVCGDVGFGKTEVALRAAFKAVLDGKQVA
                     VLVPTTILAQQHFKTFSDRLSDYPIVMGVLSRFRTKEEQAETARLLEKGQVDIVIGTH
                     RLLQKDVSFRDLGLLIVDEEHRFGVRHKEKLKAIRKTVDVLTLSATPIPRTLYMATSG
                     IRDLSVIDTPPLDRHAVRTTVVKFNDAVLTRGIRNELERSGQVFFVHNFVHNIGVVHD
                     HLARIIPEARIAVAHGQMSGSRLEKIMVDFIEKRYDILLSTNIIESGLDISNVNTIFI
                     NNAHRMGLADLYQLRGRVGRSTRQAFAFLLVPDEEKLTRDARLRLKILEELT"
BASE COUNT          573 a          809 c          723 g          446 t
ORIGIN      
        1 gacgacgcgc gtgacgcctg tctgtacata accggcaggg caggttcctt tgtctcattc
       61 ctcgtggcgc agaggaagag aaggacactg gtcctgtatg aggacgacga ctccgcggaa
      121 ctcgtcaggg acgagatagc atactacggc aacagggacg tcgttctctt cccgccctac
      181 caggaccgcg tctttgagga ggaggacgac ctaaagcgca ccggcttcct ctatcgactg
      241 gcaaaagacg acaatttcgt cgggcttttc ccggtctctg ccctcaccca caccatgcct
      301 cccccggaca ggatctccga ggacattacg accttgaagg tcggcgatgc cgtcttcccc
      361 gaggacatcc atgacaacct cgacagaaaa ggctacgaac tggcccccct ggtgcgcgag
      421 gccggccagt atgcaaaacg gggcagcatc atcgattttt tccctcccgc ctcggacagg
      481 ccggtgagat gcgagttcat aggagaccag atactctccc tgagacattt caaccctctt
      541 tcccagaggt ccgacaagga gctcgcccgc tgtgatataa cgtccatcat aaagaaggac
      601 ggcggcgggt catccaccat aatcgaccac ctcggcggac ctctcaccct cgttcacccg
      661 ggagactccc tgatcgacca cgcccttcac gccgccgatg cggcgacgaa ggaaaactgg
      721 gcacggcttc tcgccgaagc tccgcagatc gacctttcgg gcgttgtcgg cccgtcggag
      781 acccaaaaga ccctcagggc cgtctccaac gatgacctgc ggcaactctt ccttctccac
      841 aagacccgca tattccatat actccgggaa aaactgcagg gggagtggtc ccactacccc
      901 tacgtctacc tcttcgccaa cacggcgcac caggcaaaga ggctccagga gatattcgag
      961 agctatggcc tggttctgcc catcctcccg cgaatatcct accggagacg ctccagggaa
     1021 tggggcatca tcgaagggcc catccgcagg ggcttcagga ccgacgacat catcgtcctc
     1081 accgaagacg acatagccgg gcccaagaag agggtcgtca aaagacactg gggcagccgg
     1141 gatgagttcc tcgcttcttt caaagacctc accgagggcg aactcgtcgt gcatgtggac
     1201 cacggcatcg gcgtttatcg cggcatcgtc ccgctgaccg tcaacgggtt cacgaaagac
     1261 ttcctcctta tagaatatct gggcggagac aagctctatg tccccgtcga cgatctgcac
     1321 ctcgtgcaga agttcatcgg cagcgagaag ttcaagccca agatcgaccg gctcggctcg
     1381 aggttctggc tcaacacgaa gaagcgggtt cggaggcaga tcgaggacat cgccggcgag
     1441 cttctcgaga tctacgcggc gaggcagatg gcggagggcc acagctatcc gcccgaggac
     1501 gagctcttca gggagatgga gtcccgcttc gagtacgagg agacggaggg ccagatcaat
     1561 gccataaacg atgtcctcgc ggacctgaga agcacgagac ccatggacag gctcgtctgc
     1621 ggcgatgtcg gcttcggcaa gacggaagtc gccctcagag cggccttcaa ggccgtcctc
     1681 gacggaaaac aggtggccgt gctcgttccc accaccatcc tcgcccagca gcacttcaag
     1741 accttcagcg accgtctctc cgattacccc atcgtcatgg gcgtgctgtc gcgcttccgg
     1801 acgaaggagg agcaggcgga aacggcaagg ctcctcgaga aggggcaggt ggacatcgtc
     1861 atcggcaccc acaggctcct gcagaaggac gtttccttcc gcgacctcgg tcttctcatc
     1921 gtcgacgagg agcaccgctt cggtgtgaga cataaggaaa agcttaaggc catcaggaag
     1981 accgtggatg ttctgacctt gagcgccacg cccattccga ggaccctcta catggcaacc
     2041 tcgggtatca gggacctcag cgtcatcgac acgccgccgc tcgaccggca cgccgtgagg
     2101 accacggtgg tgaagttcaa cgacgcggtt ctgacgcgcg ggatacgcaa cgagctcgaa
     2161 aggtccgggc aggtcttctt cgtgcacaac ttcgttcaca acataggggt cgttcacgac
     2221 cacctggcga ggatcatacc ggaggcccgc atcgccgtcg cccacgggca gatgagcggg
     2281 agccggctgg aaaagatcat ggtggacttc atagagaagc gctacgacat actcctctcg
     2341 acgaacataa tcgaatcggg gctcgacata tccaatgtca acaccatctt catcaacaat
     2401 gcccacagga tgggcctcgc cgacctctac cagctcaggg gcagggtagg acggagcacc
     2461 cggcaggcct tcgcgttcct gcttgttccc gacgaggaga aactgacaag ggacgcccgc
     2521 ctcaggctca agatcctcga agagctgacc g
//
LOCUS       DAOPOA010000324         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_44405_length_2545_cov_2.587952, whole genome shotgun sequence.
ACCESSION   DAOPOA010000324 DAOPOA010000000
VERSION     DAOPOA010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44405_length_2545_cov_2.587952"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PKJ17_07345"
     CDS             <1..488
                     /locus_tag="PKJ17_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75823.1"
                     /translation="RLTGIIQEHHGTSLTSFFYTKAKGKEDPLLHVIEEKDFRYPGPK
                     PQTKETGIIMLADAVEAASRVLEDPTPKRIENHVQRIIEGIFLDGQLDECELTLKDLH
                     AIQKSFIAILIGIFHHRIEYPERTQNGGSDKKLPKTDEAQPKGDRQGNRGITGLFKAS
                     Q"
     gene            418..840
                     /gene="ybeY"
                     /locus_tag="PKJ17_07350"
     CDS             418..840
                     /gene="ybeY"
                     /locus_tag="PKJ17_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOD75824.1"
                     /translation="MKLNRKAIGRVTGELLASLRLHNRDLSVLLVDDGKIALMNRDLF
                     GKDRPTNVISFSYIDGMPGEAVGDIVISVERAADEARAAGIPFYERFFGLIVHGLVHI
                     LGYDHTKGASEARKMRYREKKLMEVVLGHPAYLALIDG"
     gene            833..2362
                     /gene="lnt"
                     /locus_tag="PKJ17_07355"
     CDS             833..2362
                     /gene="lnt"
                     /locus_tag="PKJ17_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOD75825.1"
                     /translation="MDSRHSGTRFSSIAARYGYALLSGALLVLVQPPVSLFPLAFIAL
                     VPLLRVIDGNDILLSFKKGFAAGVVAWLGIVYWVVVAMNSYGGINIPLSVLVLLLFAI
                     YLSLYVAIFTASCAYLEKRTSVPVYLLAAPVWVVLEYARAAVMTGFPWAFLGHSQYGF
                     LPLIQVASVTGTYFISFLIVAVNGVIFSLWVRRRVSRVWTAVVIVLVASSVAYGFVRL
                     QHRDAEPFRAAIVQGNIGQDVKWDDAFKMMTISKYYRLSFGLARGSDLIIWPETAMPF
                     IIDRDVHAYKYVRAVPAMLKSRLLFGTVGLGTERKLLNSVHYVNRDGRTAAIYNKVHL
                     VPFGEYTPIVSYFPFLANITAIGADFIPGEGHRPIETDAGRIGILVCFEGIFPYITNE
                     TVREGAQVLVNVTNDAWYNRTSAPYQHFAFYVFRAIETDRYVLRAANTGISAVIDPRG
                     RIRGKTPIFTEEVLRGAYALKDTMTFYVRYGDYFIVLCLIFLACMVVAGMYRGRGKEK
                     A"
BASE COUNT          539 a          712 c          718 g          576 t
ORIGIN      
        1 ggaggttgac gggcatcatc caggaacacc acggcaccag cctcacgagt ttcttctata
       61 cgaaggccaa gggaaaggaa gacccgcttc ttcacgtgat cgaggagaag gatttccgct
      121 accccggtcc aaagccccag acgaaggaga cggggatcat catgcttgcc gacgcggttg
      181 aagcggcgtc gcgggtcctt gaagacccca ctcccaagag gatcgagaac cacgtccagc
      241 ggatcatcga gggcatattc ctcgacggtc agctcgacga gtgcgaactg accctcaaag
      301 acctccacgc gatccagaag agcttcatcg cgatactcat cgggatcttt caccatcgta
      361 tcgaataccc ggaaaggaca caaaatggcg gttctgataa aaaactccca aagactgatg
      421 aagctcaacc gaaaggcgat cggcagggta acaggggaat tactggcctc tttaaggctt
      481 cacaataggg acctgagcgt gctgctcgtg gatgacggga agatcgccct gatgaacagg
      541 gaccttttcg gcaaagaccg ccccacgaac gtgatctcct tttcctatat cgacggtatg
      601 cccggtgagg ccgtcggaga catcgtcatt tccgtggagc gcgccgccga tgaggctcgt
      661 gcggcgggca tcccctttta cgaaaggttc ttcggcctta tcgtccacgg tctcgttcac
      721 atcctgggct atgatcacac gaaaggggcc tcggaggcga ggaagatgcg ttaccgggag
      781 aagaagctca tggaagttgt cctcgggcat cctgcctacc tggcgcttat cgatggatag
      841 ccggcattcg ggcacccgtt tttcatccat tgcggcgaga tacggctacg ccctgctgtc
      901 aggggcgctg cttgtcctcg tgcagcctcc ggtgtccctc tttccccttg cctttatcgc
      961 cctggtgccg cttctgaggg tcatcgacgg caatgacatc ctcctctcct tcaagaaggg
     1021 gttcgcggcc ggggtggtgg cctggctcgg cattgtctac tgggtcgttg tggccatgaa
     1081 cagttacggg ggcatcaata tccccttaag cgtgcttgtt ctcctgcttt tcgccatcta
     1141 tctctccttg tatgtggcga tcttcacggc ctcatgcgcg tacctggaga agaggacctc
     1201 cgttccggtg tacctcctcg cggcgcccgt gtgggtggtg ctcgagtacg cgcgcgcagc
     1261 ggtcatgacg ggtttcccat gggcgttcct tggccactcg caatacggtt tccttcctct
     1321 catacaggtg gcttccgtca cggggacgta tttcatctcc ttcctgatag tggcggtgaa
     1381 cggcgtgatc ttttctctgt gggtccgaag aagggtgtcc cgtgtatgga cggcggtggt
     1441 gatcgttctt gtcgcctctt ccgttgccta cggtttcgtg aggctccagc acagggacgc
     1501 cgagccgttc agggcggcga tcgtgcaagg caacatcggc caggacgtga aatgggacga
     1561 cgccttcaag atgatgacca tcagcaagta ctaccggctg agcttcggcc tcgcgcgggg
     1621 ctcggatcta attatttggc ccgaaacggc gatgcctttc atcatagacc gtgacgttca
     1681 tgcgtacaag tatgtgaggg cggtgcccgc gatgctcaag tcccggctcc ttttcggaac
     1741 ggtggggctc ggaacggaaa gaaagctctt gaacagcgtc cactacgtca acagggacgg
     1801 acggactgcc gcgatataca acaaggtcca cctcgtccct tttggagaat acaccccgat
     1861 cgtttcctat ttccccttcc ttgcgaatat caccgccatc ggcgcggact tcatccccgg
     1921 tgaggggcac aggcctatcg aaacggatgc gggaaggatc ggcatcctcg tctgtttcga
     1981 gggcatattc ccctatatca ccaatgaaac ggtgagggag ggggctcaag tcctcgtgaa
     2041 cgtcaccaac gatgcctggt acaacaggac gtcggcgccg tatcaacact tcgcttttta
     2101 tgtcttccgg gcaatagaaa cggacaggta tgtattgcgg gccgccaaca cgggcatcag
     2161 cgccgtcatc gaccccaggg gccggatccg ggggaagacc cccatcttca cggaggaggt
     2221 cctgaggggc gcctatgcgc tcaaggacac catgacgttc tacgtacggt atggagacta
     2281 ttttatcgtc ctgtgcctta tctttctggc ctgcatggtg gttgcgggga tgtaccgggg
     2341 gcgtggaaag gaaaaggctt aataaccaat gaccaaatcc caatgaccaa tgaaaaagat
     2401 aatgaccaat tcaccaataa ccaattaaac aaaggccagt tccatacgga ctgtctgcat
     2461 gattttggtt attggtcatt gtcttctcgt tgccagtttt tccttgacaa aagggcatcg
     2521 gtaaaataat ttatgcaaaa tttcc
//
LOCUS       DAOPOA010000325         2541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_44497_length_2541_cov_3.413918, whole genome shotgun sequence.
ACCESSION   DAOPOA010000325 DAOPOA010000000
VERSION     DAOPOA010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44497_length_2541_cov_3.413918"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="PKJ17_07360"
     CDS             <1..1274
                     /locus_tag="PKJ17_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HOD75826.1"
                     /translation="PHYETANQVYLVVERDYLNQVLSTYLSANQRSVGSMLNDLAASA
                     AGDLDRVLGILDAIPSYGQVAGAYEQIAPRSNQVDFTMGVSSAVFQAGNVAQRLSEIR
                     LGAWGTNPGSTFIRNSGFIREGRKPILIASTGSDLAGMLPDREEEKWGIFAKGNAVSG
                     DQRDTPDQMGYSFTSAGLTVGMDYRFNRNIAAGLLFGYTGSRAEVDDNGSRMTMDGYT
                     FGAYGTWYRGRFYVDGQASYGWADYRNTRRIVFPGLDRTASSSPGGSQFTLYGGTGYE
                     IPAGRWTIVPNLSLQYIDLGIDSYTESGAGSLDLNVDRQRSQSLQGNFGGKLYYTWET
                     PKSVVMPGITVAWGHEFMRDSRDITSRLVQGSSPFSIDTVSPDRNFLLAGAGVSMFTH
                     GGTSFHIGYDVQVGENRYTVHSINGVMRMFF"
     gene            1329..>2541
                     /locus_tag="PKJ17_07365"
     CDS             1329..>2541
                     /locus_tag="PKJ17_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD75827.1"
                     /translation="MTLKDRSAQIGLIVGVSFALLMSKIDSSIVNISLPVISQEFHVT
                     TSQASWIIISYLLILTCTLLLFGKICDLINIKRFFMAGYIIFVGGSLMCGLSPNLPVL
                     VASRVVQALGGAILNIAAYAIVSRFLPPGGIGWAFGILTAVNAVGVSIGAPLGGFITV
                     HLSWRWIFFINIPIGCVAILQVIRAIPNDPVTGRRRSRIDYAGAVLSMLSLLSLLFTL
                     NLGSELGWLSLPIVLFFLGFAVFAFLFIVVEKRAADPLVHLDIFSDRSFLLTLCATVT
                     ALMILSGTGILMPFYLEQIKGMTADKVGLFMLIYTGIFAVVSSGFAGKLSDRITPAKI
                     CAAAIFSVALSALFFAFTLALPGYTFVIISFVWMGASLGFFMPSNNNQVMNKATPETK
                     GEVSGLLNTAVN"
BASE COUNT          471 a          814 c          736 g          520 t
ORIGIN      
        1 aaccccacta cgagaccgcg aaccaggtct acctcgtcgt ggagcgcgac tacctgaacc
       61 aggttctgtc gacatacctc agcgcgaacc agagatccgt cggctccatg ctgaacgatt
      121 tggccgcatc ggcagcaggc gatctcgaca gggtcctcgg catccttgac gccatcccct
      181 cgtacggtca ggtggcgggc gcctacgagc agatcgcccc caggtcgaac caggtagact
      241 tcaccatggg tgtgagctcc gcggtcttcc aggcgggcaa cgtggcccag aggttgtccg
      301 aaatccggct cggtgcgtgg gggacgaacc ccggcagcac cttcatcagg aacagcggtt
      361 tcatccgcga gggaaggaag cccatcctca tcgccagcac gggttccgac ctcgcgggca
      421 tgctccccga ccgcgaggag gaaaagtggg gtatcttcgc gaaggggaac gccgtctccg
      481 gggaccagag ggacaccccc gatcagatgg gatacagctt cacgagcgcc gggctcaccg
      541 tgggcatgga ctatcgcttt aaccggaaca ttgccgcggg tcttctcttc ggatacaccg
      601 gctcccgggc cgaggtcgac gacaacggca gcaggatgac gatggacggc tacaccttcg
      661 gcgcctacgg cacgtggtac aggggaaggt tctacgtgga cggacaggcg agctacgggt
      721 gggcggacta caggaacacg cggcgcatcg tctttcccgg cctcgaccgc acggcgagct
      781 ccagcccggg aggctcgcag ttcaccctct acgggggcac gggttacgag atccccgcgg
      841 gcagatggac catcgtgccc aacctctccc tccagtacat cgatcttggc atagacagct
      901 acaccgagtc aggggcgggg tctctcgatc ttaacgtgga caggcagagg agccagtctc
      961 tccaggggaa ctttgggggt aagctgtact acacgtggga gaccccgaag agcgtcgtaa
     1021 tgccgggcat caccgtcgcc tggggccacg agttcatgag ggactcccgg gacatcacat
     1081 cccgccttgt ccagggaagc tcgccctttt ccatcgacac ggtctccccg gacaggaact
     1141 ttctccttgc cggcgcgggg gtatcgatgt tcacccacgg cgggacgtcc tttcacatcg
     1201 ggtacgatgt acaggtcgga gagaacaggt acaccgtcca cagcataaac ggcgtcatga
     1261 ggatgttttt ctagaggacc ttaacgttcc gggaagaacg gccgattacc attgttggtg
     1321 aggccagcat gacactaaag gaccggtccg cgcagatagg gctcattgtg ggcgtctctt
     1381 tcgccctgct catgagcaag atagacagct ccatcgtcaa catatccctt ccggtgatat
     1441 cgcaggagtt ccacgtcacc accagccagg cgtcgtggat catcatatcc tatctcctga
     1501 tcctgacgtg cacgctcctt ctcttcggga agatatgcga cctcatcaac ataaagaggt
     1561 tcttcatggc cggctatatc atattcgtgg gagggtcgct gatgtgcggg ctctccccca
     1621 acctgccggt gctggtggcg tcgcgggttg tccaggccct cggcggggca atactcaata
     1681 tcgccgccta tgccatagtc tcgcggttcc ttcccccggg cggcatcggg tgggccttcg
     1741 gcatactgac ggccgtcaac gccgtcgggg tctccatcgg ggcgccgctg ggcggcttca
     1801 tcaccgtcca cctttcctgg cggtggattt tcttcatcaa catccccatc ggctgcgttg
     1861 ctatcctgca ggtgataagg gcgataccca acgaccccgt caccgggcgg aggcgctcga
     1921 ggatcgacta cgcgggggcg gtcctcagca tgctctcgct cctcagcctt cttttcacct
     1981 tgaacctcgg cagcgagctt ggctggttgt cgctgcccat cgtccttttc ttcctggggt
     2041 tcgccgtctt tgcctttctc ttcatcgttg tggagaagcg cgccgcggat cccctcgtcc
     2101 acctcgacat cttttccgac cgcagtttcc tgctcaccct ctgcgcgacg gtgacggcgc
     2161 tcatgatact gagcggaacg ggtatcctca tgccctttta cctcgaacaa ataaagggca
     2221 tgacggcgga caaggtcggc ctcttcatgc tcatctacac cggcatcttc gccgtcgtct
     2281 cctccgggtt cgccggcaag ctctcggaca ggataacgcc cgcgaagata tgcgccgcgg
     2341 ccatcttcag cgtggccctc tcggctctct tcttcgcatt tactctcgcg ctaccgggct
     2401 acaccttcgt catcatctcc ttcgtgtgga tgggcgcgtc cttaggtttc ttcatgcctt
     2461 ccaacaacaa ccaggtcatg aacaaggcga cgcccgagac aaagggagag gtttcaggtc
     2521 tcctcaatac cgcggtcaac c
//
LOCUS       DAOPOA010000326         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_44762_length_2529_cov_4.392886, whole genome shotgun sequence.
ACCESSION   DAOPOA010000326 DAOPOA010000000
VERSION     DAOPOA010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44762_length_2529_cov_4.392886"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2069
                     /locus_tag="PKJ17_07370"
     CDS             <1..2069
                     /locus_tag="PKJ17_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOD75828.1"
                     /translation="GTTETSIPAVFSGGDCVSGPDIAIGAIGAGKRAALQIDEYLRTG
                     ICTPVPEPYNCSKGNWRTIPRDEYKWVTPSERLDAPTLAPEIRKTGFQESTRTWDSES
                     AMQEAARCLACGCTERYTCVLRSHASEYGVEFDQPTPARPLPIDENHPFIVRDPGKCI
                     LCGLCLKICREMEGSSALSFYETDNVLTIGPNDHRPLDMTVCVACGHCATACPTGALS
                     FKPVIPRIYRALHNPNLTVVAQIAPAVRAAFGQHYGIDPTSAMSVLSAGLKEVGFDFV
                     FDTCWAADLTIMEEGTEFLTRVAEGGVLPQITSCCPAWVNYCEKMAPDILPHLSSCRS
                     PQQMFGSVMKHYFARELKVEPGRLFFVSIMPCNAKKYEARRPEFNREGLADVDAVLTT
                     TEVIRLFEERGMDPKTFKPVPVDAFFGKASGAGIIFGASGGVAEAALRLAAERVIGKR
                     MESFTYEEVRGLKGVKETTVGLGETKVRLAVVSGLTNAQDLIDRIRSGDAPYDLIEVM
                     ACPGGCINGSGNPAPKLTSDPGDRLEVLYRLDEAASIKKSQDNPTIQAIYTNWLGEPD
                     GTLSHHNLHTTYRRRSMRVQESVEELLRDNVPIDVGVCLGTSCYLKGSFHLLEGLSAE
                     LRKRGLLDRFRIKARFCTDQCSGGPNVVVGANVINRVDPNDPVAFIDAHLIPALDAGG
                     GKGTAS"
     gene            2348..>2529
                     /locus_tag="PKJ17_07375"
     CDS             2348..>2529
                     /locus_tag="PKJ17_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75829.1"
                     /translation="MLKDVALKHRMILGGVAAVLVPFLLAGTIVYVLLSGSLMEMTRE
                     RSVRMAMDMAALIDANL"
BASE COUNT          507 a          821 c          753 g          448 t
ORIGIN      
        1 ccggcacaac ggagacctcc attcccgctg tcttctccgg gggagactgc gtgagcggtc
       61 ccgatatcgc cataggtgcc atcggagcgg gcaagcgggc ggccctgcag atagacgaat
      121 acctccgcac cgggatatgc acccctgtcc cggaacccta caactgctcg aaaggaaact
      181 ggcggacgat accccgggat gaatacaaat gggtaacccc gtcggaaaga ctcgacgcac
      241 ccaccctcgc acccgagatc aggaagacgg gctttcagga gtcgacgcgc acctgggaca
      301 gcgagagcgc aatgcaggaa gccgcgcgat gcctcgcctg cggatgcacg gagcgctaca
      361 catgtgtcct cagaagccac gccagcgaat acggcgtcga gttcgatcag ccgacgccgg
      421 cacggccgct tcccatcgac gagaaccacc cctttattgt ccgcgacccg gggaagtgca
      481 tcctctgcgg tctgtgtctc aagatatgcc gcgaaatgga gggctcgtcg gccctgtcat
      541 tctacgagac ggacaacgtc ctgacgatag ggcccaacga ccaccggccg cttgatatga
      601 ccgtctgcgt ggcctgcggc cactgcgcca cggcatgccc cacaggcgcc ctgtccttca
      661 agcctgtcat cccccggatc taccgcgccc ttcacaaccc gaacctcacc gtcgtggcgc
      721 agatagcgcc ggcggtgcgg gccgctttcg gccagcatta cggtatagac ccgacctcgg
      781 ccatgtccgt cctttcggca gggctcaaag aggttggatt cgattttgtt ttcgatacgt
      841 gctgggccgc ggacctcacc atcatggaag agggcacgga gtttctgacc cgggtggccg
      901 agggcggagt ccttccccag ataacctcct gctgccccgc ctgggtgaac tattgtgaga
      961 agatggcccc cgacatcctt ccccatcttt catcctgccg gtctccccag cagatgttcg
     1021 gttccgtcat gaagcactac ttcgcgcggg agctcaaggt cgaacccgga cgccttttct
     1081 tcgtttccat aatgccctgc aacgcgaaga aatacgaggc gcggcggccg gaattcaacc
     1141 gggaaggcct ggctgacgtc gacgcggtac tcaccacgac ggaggtcatc aggctcttcg
     1201 aggagcgggg aatggatccg aagaccttca agcccgtccc cgtagacgcc ttcttcggca
     1261 aggcaagcgg agcgggcatc atcttcggcg cttccggcgg tgtcgccgag gcggccctgc
     1321 gtctcgcagc tgaacgggtg atcggaaagc gcatggaaag cttcacctac gaggaggtga
     1381 ggggcctgaa aggcgtcaag gagaccaccg tcggcctcgg ggaaacaaag gtgcgcctcg
     1441 ccgttgtgag cgggctcacg aacgcgcagg acctcatcga ccgtatacgt tccggggacg
     1501 ccccctacga cctcatcgag gtcatggcct gtccgggcgg ctgcatcaac ggctcgggaa
     1561 acccggctcc aaaactcacg agcgaccccg gggacaggct cgaggtcctc tatcgcctcg
     1621 atgaggcggc atccataaag aagagccagg acaacccaac gatccaggcc atctacacga
     1681 actggctcgg cgagcccgac gggaccctgt cccatcacaa cctccacacc acctaccgcc
     1741 ggcgttccat gcgcgttcag gaatccgtcg aggaactcct tcgggacaat gttcccatcg
     1801 acgtgggcgt ctgtctcggc acgagttgct acctcaaggg ttccttccac ctcctcgaag
     1861 ggctctcggc ggaactgcgc aagcggggcc tcctggatcg attcaggata aaggcgcgct
     1921 tctgcaccga ccagtgttcc ggcggcccca acgtcgtcgt gggggcgaac gtgataaatc
     1981 gcgtagaccc caatgacccg gtggccttca tcgacgccca cctcattccc gccctcgacg
     2041 cggggggcgg caaaggcacg gcgtcttaaa gccacgcccc ggggctctgc cggggccatg
     2101 cggaccgggg tattaacgta accgtcaaat ggtccgacaa tgttaagaag ggtacccccg
     2161 atgcggaaac ccccgggtaa accgaacgac gcggtgcaga cctcccgtcc cgccgcagaa
     2221 ggatatcact tcctgcattc cgggcaccac cgggagcgct tcctgcggat ctgtcttgta
     2281 catatttcgg tattgcgtgt accatagtgt tgggcagcat gaagaacgtt gtcggatgtg
     2341 gtgaaggatg ctgaaggacg tggccctgaa acacaggatg atacttgggg gcgtagccgc
     2401 cgtcctcgtt cctttcctgc tggcaggaac gatcgtgtac gtccttctct ccggctccct
     2461 catggagatg acacgggaga gatcggtccg catggcaatg gatatggccg ccctgatcga
     2521 tgcgaacct
//
LOCUS       DAOPOA010000327         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_44763_length_2529_cov_4.379143, whole genome shotgun sequence.
ACCESSION   DAOPOA010000327 DAOPOA010000000
VERSION     DAOPOA010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44763_length_2529_cov_4.379143"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PKJ17_07380"
     CDS             complement(<1..1001)
                     /locus_tag="PKJ17_07380"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD75830.1"
                     /translation="MGFSSFKLHPKIAASIKALGYQVPTPIQVQAIPPVLAGRDVMGL
                     AQTGTGKTAAFVLPILEHLLPGPRGRVRSLIIAPTRELAEQIHVSIGELGRNTHLKSC
                     TVYGGVGVNPQVSKLRSGVDIVVACPGRLLDHLTQKTIDLEDLEILVIDEADRMFDMG
                     FLPDIRRIIRQLPVKRQTLMFSATMAPSIRKLTDEVLSDPVTVKIGHTNPADTVSHAL
                     FPVEQHLKTRMLMELLRQTDTESILIFTRTKYRAKRIGQELQKAGYRAASLQGNLSQN
                     KRQEALDGFRDGSYQILVATDIAARGIDVSNISHVINYDMPDTTDAYTHRIGRTGRAA
                     "
     gene            complement(1194..1472)
                     /locus_tag="PKJ17_07385"
     CDS             complement(1194..1472)
                     /locus_tag="PKJ17_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOD75831.1"
                     /translation="MTDSAAIFVYILECSDKTLYCGWTNNLRKRLEEHAAGKAGAKYT
                     RGRRPVRLVYREPCPTRSDALKREREIKRLSRAGKLLLIEKTSEGRLI"
     gene            complement(1478..>2529)
                     /locus_tag="PKJ17_07390"
     CDS             complement(1478..>2529)
                     /locus_tag="PKJ17_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD75832.1"
                     /translation="RMREERAARQEQTGLSRFSIEEAERSGRMVVDARGVFFAWEDRK
                     IIDGFSATIIRGDRVGIIGPNGCGKTTLLRILLGQLVPDEGKVRLGTNVAVAHFDQLR
                     SQLDETRTLRENIGGGNDMIITGSGSRHVVGYLQDLLFSPERTMSPVGSLSGGERNRL
                     LLAKLFIVPSNVLVLDEPTNDLDTETLELLEEKLLEYNGTILMVSHDRAFLNNVVTST
                     IVFEEDGGLREYVGGYDDWLRQANSALAAERTVPKEQKQKKAKAPQEKKRLSFKEARE
                     LESLPPLIEALEEEKRQLTETLNSPEFYAGRDLERIRTVNDRWGALEKELNAAYHRWD
                     ELEELKAALAGPSER"
BASE COUNT          447 a          748 c          772 g          562 t
ORIGIN      
        1 gccgcgcggc cggtgcggcc gatgcggtgg gtgtatgcgt ccgtggtgtc cggcatatcg
       61 tagttgatca cgtgggatat gttcgataca tcgatgcccc gcgccgcgat atccgtcgcc
      121 acgaggatct ggtaggaccc gtcacggaag ccgtcgagag cctcctgacg cttgttctgt
      181 gaaaggttcc cctgcagtga ggcggccctg tatccggcct tctgcaattc ctggccgatg
      241 cgttttgcac ggtacttggt gcgtgtaaag ataaggatgg attccgtatc ggtctgccgg
      301 agaagctcca tgagcatcct ggtcttcagg tgctgctcca cggggaagag cgcgtgggac
      361 accgtgtcgg ccggattcgt atggccgatc tttaccgtga cggggtcgga aagcacctcg
      421 tcggtaagct tgcggatgct cggggccatg gttgcggaga acatgagcgt ctgccgtttc
      481 acgggcagct gcctgatgat ccgcctgatg tcggggagga agcccatgtc gaacatccgg
      541 tccgcctcat cgatgacgag gatctcgagg tcctcgaggt cgatcgtctt ttgtgtaagg
      601 tgatcgagga ggcggccggg acaggccacg acaatatcga cgccggaacg gagcttgctg
      661 acctgcgggt taacgcccac accaccgtag acggtgcagc tcttcaaatg ggtgttgcgc
      721 cccagctctc cgatggacac gtgtatctgt tccgccagtt cccgtgtggg tgcgatgatg
      781 agcgagcgca cccttccccg cgggccgggg aggagatgct cgagtatggg cagcacgaag
      841 gcggccgttt tgcccgtacc ggtctgtgca aggcccatga cgtccctgcc ggcgaggacc
      901 ggcgggatcg cctgcacctg aatgggggtg gggacctgat agccgagcgc ctttatactg
      961 gcggctattt ttggatgaag cttgaatgaa ctgaaaccca agagcacctt ctttcaaata
     1021 agatcgacag ccaacgggac aggagagccc gaaccggcca ggactgttcc ggggagtcat
     1081 cgggctcaat gaaattgtct gttatgggaa cgtttcggac agaaaatctt cacgcggcca
     1141 gggatcgttg cttcgcagcc cggtgaacac tataagatat agagcggccc ctgtcaaata
     1201 agtcttcctt cggacgtttt ttctatgagg agcagcttcc cggcacggga gagcctcttt
     1261 atctccctct cccttttcag ggcatcggat ctcgtcgggc agggctccct gtagacgagt
     1321 cgaaccggcc ggcggccacg cgtgtatttg gcccccgcct ttcccgcggc atgctcttca
     1381 agacgttttc taagattgtt cgtccagccg caatagaggg ttttgtcgga acattcgagg
     1441 atgtagacga atatcgcagc gctgtctgtc acggcggtca tcgctccgat gggccggcga
     1501 gggctgcctt cagttcttcc agttcgtccc agcgatgata ggcggcattg agttccttct
     1561 cgagggcacc ccagcggtca ttgacggtcc ttatcctttc gaggtcgcgt cccgcataga
     1621 actcgggtga attgagggtc tccgtcaatt gccgcttttc ctcctccagg gcctcgatga
     1681 gtggcggcag ggattcgagc tcccgggcct ctttgaagga caaccgcttc ttttcctgag
     1741 gcgccttcgc cttcttctgt ttttgctcct tcggcaccgt ccgctccgcc gccagggcag
     1801 agtttgcctg cctgagccag tcatcgtagc cgccgacata ttccctcaag cccccatcct
     1861 cttcgaagac gatcgtcgag gtcaccacat tgttcaggaa tgccctgtca tggctcacca
     1921 tcaggatggt gccgttgtat tcgaggagtt tttcttcgag gagctccagc gtttccgtgt
     1981 cgaggtcgtt cgtaggctcg tcgaggacga ggacattgga agggacgatg aagagcttcg
     2041 cgagaagcag gcggttgcgc tcgccgccgg aaagggagcc gacaggcgac attgtccgct
     2101 cgggagaaaa gagaaggtcc tggaggtacc ccaccacgtg ccgggagccg ctcccggtga
     2161 tgatcatgtc gttgccgccg ccgatgttct ctctcagcgt tctcgtctca tcgagctggg
     2221 agcgcagctg gtcgaagtgc gcaacggcca cgttcgtccc cagcctgacc tttccctcat
     2281 ctggcacgag ctgaccgagg agtatcctga gaagggttgt cttgccgcac ccgttggggc
     2341 ctatgatccc cacccgatcc ccccggatga tggtggcgga gaagccgtcg atgatcttcc
     2401 tgtcctccca ggcaaaaaag acgccccttg catccacaac catcctgccg ctgcgttccg
     2461 cctcctcgat ggagaaccgg gacaggcccg tttgctcctg ccgcgccgcc cgttcctccc
     2521 gcatccgca
//
LOCUS       DAOPOA010000328         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_44765_length_2529_cov_4.008084, whole genome shotgun sequence.
ACCESSION   DAOPOA010000328 DAOPOA010000000
VERSION     DAOPOA010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44765_length_2529_cov_4.008084"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PKJ17_07395"
     CDS             <1..1113
                     /locus_tag="PKJ17_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOD75833.1"
                     /translation="TIFRIPAFTLATAGQLPFYVGLGLLCALVGYFYVKVFYGTRDRF
                     FRRLDMPKWLKPALGGLMLGVLAFFLPEILAGGYQWIQSAIDGHLAVGLMIALVFGKI
                     VATSLTISSGGSGGVFAPSLFIGSMLGGFYGDICGRIFPEIVTQPAAFVLVGMGGFFA
                     GVAKTPIAALIMVAEMTGGYSLILPMMIVSALAYLLLGKTTLYEKQVATRVDSPAHVG
                     EYSVDIMDHLLVKDAVVPDRKIETIPEEMGFELMVDLMADSYQQDFPVVDRNGNLVGI
                     ISMTDLRTAMADSSLHRLLVARDIAVTGVTAVTPEDSLNTALQIMADMNVRELPVVER
                     GDPGRIVSMVSRRDITRAYHNEMERRGSRRTGGAKG"
     gene            complement(1378..2358)
                     /locus_tag="PKJ17_07400"
     CDS             complement(1378..2358)
                     /locus_tag="PKJ17_07400"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HOD75834.1"
                     /translation="MERSLLIAISLLMLLPCLSRASSDPVRFFRMGDGKISIRNEHSG
                     REATVNLFAADGKLNENALDAIDRVFGFTDGRKGEHISLRLLVLLDHFTDRVAPGKTI
                     HLVSGYRSPAYNQKLKKSGGNVARTSTHMDGMALDFYLDGINGKLLWEIIRRGNCCGV
                     GHYGGKNIHLDSGKPRFWEAATSKTGTRESEFNRKLYFSTEYDRYRQGEKVRFFLTSL
                     SDFPFGVRKTASLLRDGDGENGMTALMIEGRDGEECVMINDRNASRSIRGTLPAGLEP
                     GRYRIRFDFCKRPFEQMPTTTSSNEIEIVKDWKTVQEFQGSRVQEEKRPR"
BASE COUNT          487 a          714 c          766 g          562 t
ORIGIN      
        1 acgatctttc ggatccccgc attcactctt gcaactgccg gacagttgcc gttctatgtc
       61 gggctgggac ttctctgcgc tcttgtgggc tatttctatg ttaaagtttt ctacggtacg
      121 agggaccggt tcttcaggcg ccttgacatg ccgaagtggc tgaagcccgc cctgggaggt
      181 ctgatgctcg gtgtcctcgc cttcttcctg cccgagatcc tcgcgggagg gtaccaatgg
      241 atccagtcgg ccatcgacgg gcaccttgcg gtaggtctca tgatagccct cgttttcggc
      301 aagatcgtgg ccacgtcgct gaccatcagc tccggcggga gcgggggtgt gttcgccccg
      361 tcccttttca tcggttccat gctcgggggt ttctacgggg acatctgtgg aaggatattt
      421 ccggagatcg tgacccagcc cgccgccttt gtcctcgtcg gcatgggagg tttcttcgcc
      481 ggggtggcaa aaaccccgat cgccgcgctc atcatggttg ccgagatgac cggaggctac
      541 agcctcatcc ttcccatgat gatcgtgtcc gcccttgcgt accttcttct cgggaagacg
      601 accctctatg aaaagcaggt ggccacgcgg gtcgattctc ccgctcacgt gggggaatac
      661 tcggtggaca tcatggacca cctcctggtc aaggacgcgg tggtgcccga caggaagatc
      721 gagaccatcc ccgaagagat gggattcgag ctcatggtgg acctcatggc ggattcatat
      781 cagcaggatt ttccggttgt ggacaggaac ggcaaccttg tcgggatcat ctccatgacc
      841 gatcttcgca ccgccatggc ggactcttcg ctccatcgcc tccttgtcgc gcgggacata
      901 gcggttacgg gcgtgacggc ggtcaccccg gaggactccc tgaacacggc cttgcaaatc
      961 atggctgata tgaacgtgcg ggaactgccg gtggtggaga ggggggaccc gggcaggatc
     1021 gtgtcaatgg taagcagaag ggatatcacc cgcgcatacc acaatgagat ggaacgcaga
     1081 ggctcccgca ggacgggagg ggcgaagggc taggccccga cacgatcgaa gcggacggaa
     1141 gccgtgggaa ttgaggggtc tgtcgtctgt ccttctcgat gcggggcagg tgaactggca
     1201 gaaagatgaa ggtcgataaa gcgggctgag gggtaacgcg gggggggaca cgctgcttct
     1261 tccccgggcg gccacgcttc ggatcacacc cggcaaaaaa agttcaagag ttccagagtc
     1321 agagacgcgt attccgaccc gaccgccccg tcaaccggaa cggaacgctg gacgatctta
     1381 ccggggcctc ttttcttctt gaactcttga accctggaac tcttgaaccg tcttccagtc
     1441 tttgacgatc tctatctcgt tcgatgaggt tgtcgttggc atctgttcaa agggtctctt
     1501 gcagaaatcg aaccggatcc tgtatcggcc cggttccagt cccgcgggga gcgttccgcg
     1561 gatcgatcgg gacgcgttcc ggtcgtttat catgacgcac tcttccccgt cccgcccttc
     1621 gatcatcagc gccgtcatgc cgttctctcc gtcgccgtcc ctgagcaatg aggcggtctt
     1681 gcggaccccg aaggggaagt cgctcaggga cgtcagaaag aaacggacct tttccccctg
     1741 tctgtagcgg tcgtattcgg tcgagaaata gagcttgcgg ttgaattcgc tttccctggt
     1801 gccggtcttt gacgttgccg cttcccagaa gcggggtttt ccggaatcga ggtgtatgtt
     1861 cttgcccccg taatggccga cgccgcagca attccccctc cggatgatct cccacaggag
     1921 cttgccgttt atcccgtcaa ggtagaagtc cagcgccatg ccgtccatgt gggtgcttgt
     1981 cctcgccacg ttgccgccgg acttcttcag cttctggtta tatgcggggc ttctgtagcc
     2041 cgagacgagg tggatggtct tgcccggggc gaccctgtcg gtgaaatgat cgagcagaac
     2101 gagaagcctc agggagatgt gttccccctt ccgaccgtcg gtaaaaccga agaccctgtc
     2161 gatggcgtcg agcgcgttct cgttgagctt gccatcggca gcaaagaggt tcactgttgc
     2221 ttcccgtccg ctgtgctcgt tccgtatgct tatcttcccg tcccccatcc ggaagaaacg
     2281 gacagggtcg gacgatgccc tgctcaggca ggggaggagc atgagcagtg aaatggcgat
     2341 caacaaagac cgttccatga gtgaaaggcg tgagtctgat atgtcggacg aaatcatgca
     2401 gaattataat aggtggtcaa aggatatgca acccaaagac tgttccaggg ttcaagagtt
     2461 caaaggttca agagtaaaag acaaaaaggg caccggatgc cttcggccgg tgtgcggatt
     2521 gcgttgaca
//
LOCUS       DAOPOA010000329         2519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_44993_length_2519_cov_4.000000, whole genome shotgun sequence.
ACCESSION   DAOPOA010000329 DAOPOA010000000
VERSION     DAOPOA010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44993_length_2519_cov_4.000000"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1601)
                     /gene="gatB"
                     /locus_tag="PKJ17_07405"
     CDS             complement(153..1601)
                     /gene="gatB"
                     /locus_tag="PKJ17_07405"
                     /inference="COORDINATES: protein motif:HMM:NF004015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOD75835.1"
                     /translation="MDYGDYEGVMGLEVHAHLLTESKIFCGCSTKFGAEPNSHTCPTC
                     MGLPGALPVLNRKVVEFAIKLGLALHCSINPRSVFARKNYYYPDLPKGYQISQYEEPI
                     CGNGWLDIVMDGKTKRIRILRIHMEEDAGKLVHERTIDTSSYSMVDYNRSSTPLLEIV
                     SEPDLRTPEEATAYLKTLRDILMYLEICDGNMEEGSFRCDANVSVRKKGDEAFGTRAE
                     LKNLNSFRYIERSLDYEIDRQIALIKKGGSVVQETRLFNAEQGVTYSMRGKEEAHDYR
                     YFPEPDLLPVTVDETWVEEVRKTLPELPMEKMERFMKDYGLPRYDVEILASDRELASY
                     FEAVLKLFPEAKTVSNFIMTELLRELKDGNVSPKDSPVTPARLAELLTLIKDGTISNK
                     IGKEIFPEIYRTGASPRDYVKQKGLLQISDEGAIEGIIDAVIARSPKEVADFRAGKEK
                     LIGFFVGQVMRETKGKANPALLNELLLKKLKA"
     gene            1753..>2519
                     /gene="mtgA"
                     /locus_tag="PKJ17_07410"
     CDS             1753..>2519
                     /gene="mtgA"
                     /locus_tag="PKJ17_07410"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOD75836.1"
                     /translation="MATRKVKKKKPGKLLRFIKVTIVLVVLLVVGFAGFYMVYPDVSK
                     LKKQNPGKTSFMEYREAEYRAKGNKIRIQSKWVPLRSISPYLMKAVLIAEDDKFWSHH
                     GFDTEAIQKALEKNLEAGRFKLGGSTISQQLTKNLYLTPSKNPVRKLKEAIITWRLER
                     ALSKRRILELYLNVVEWGEGIFGAEAAARRHFGKSASALTAEEAAKLAAALPNPRRYR
                     VDGTSRYVERRAKIIYTIMVRRGIVVPDYEEVTTTPP"
BASE COUNT          509 a          739 c          741 g          530 t
ORIGIN      
        1 ggtaaagaac gccgttcttc cagtaaatat gatccgtcat gcgtcacctc tttctgttga
       61 tggtcatccc ggacccgatc cgggatccag aggttcttcc gttgcgcgct tcttcctgga
      121 ttccggccta aagccggaat gacgaaaaaa atttaggcct tcagcttttt cagcaggagt
      181 tcgttgagga gcgccgggtt ggccttgcct ttcgtttccc tcatgacctg gccgacgaag
      241 aagccgatga gcttctcctt gccggccctg aagtcggcca cttccttcgg tgaccgggcg
      301 atgaccgcgt cgatgatgcc ctcgatggcc ccctcgtcgg agatctggag caggcccttc
      361 tgcttcacgt agtcacgcgg cgaggcgccg gtccggtaga tctccgggaa tatctccttg
      421 ccgatcttgt tgctgatggt gccgtccttg atgagcgtga gaagttcggc aagccgtgcc
      481 ggcgtaacgg gggagtcctt cggggaaacg ttgccgtcct tcagttccct caacagctcc
      541 gtcatgatga agttgcttac cgttttcgcc tcgggaaaga gcttgagaac cgcctcgaaa
      601 tacgaagcca gctccctgtc ggaggcgagt atctccacgt cgtaccgggg gaggccgtag
      661 tctttcatga agcgttccat cttctccatg gggagttcgg gcagggtttt ccgcacctct
      721 tcgacccatg tttcgtccac ggtcaccgga agaaggtcag gttcggggaa atagcggtag
      781 tcgtgcgcct cctccttgcc gcgcatggaa taggtgacgc cctgctcggc gttgaagagg
      841 cgtgtctcct gaacgacgct cccacccttc ttgatgaggg cgatctggcg gtcgatctcg
      901 tagtcgaggg acctttcgat gtagcggaag gagttcaagt tcttaagctc cgcccgggtg
      961 ccgaaggcct cgtcgccctt cttgcgcacc gagacgttgg cgtcgcagcg gaagctgcct
     1021 tcctccatgt tgccgtcgca tatctcaagg tacatgagga tgtcgcgcag ggtcttgagg
     1081 tacgccgtcg cctcctccgg tgtcctcagg tcgggttcgc tgacgatctc gaggagcggc
     1141 gtgctcgagc ggttgtagtc caccatgctg taactgctcg tgtcgatggt ccgctcgtgg
     1201 acgagcttgc ccgcgtcctc ctccatgtgg atgcgcagga tgcggatgcg cttcgtcttc
     1261 ccgtccatga cgatgtcgag ccatccgttg ccgcagatgg gctcttcgta ttgagagatc
     1321 tggtacccct tcggcaggtc gggatagtag tagttcttcc gggcgaagac gctcctggga
     1381 ttgatggagc agtgaagggc gagccccagc ttgatggcga actcgacgac cttcctgttg
     1441 aggaccggca gggcacccgg caggcccatg caggtggggc aggtatggct gttcggctcg
     1501 gccccgaact tcgtcgaaca gccgcagaat atcttgctct ccgtaagaag atgggcatgg
     1561 acctcgagac ccattacccc ttcgtagtca ccataatcca cggcgcacct ctctgactgc
     1621 tttctatcgc ttacaaatga ttgaaactat acaacaggta acggtgtcat ttcaaaacaa
     1681 attgccccct taagggagaa agggaccttc caatggcgcc cgcgatatga taagaagaga
     1741 tgggtgcagg aattggcaac aagaaaggtc aaaaagaaga agccggggaa actcctgcgc
     1801 ttcatcaagg tcaccatcgt tctcgtcgtc cttctcgtgg tggggttcgc gggtttctac
     1861 atggtctacc ccgacgtgtc gaagctgaaa aagcagaacc cgggtaagac gtccttcatg
     1921 gaataccggg aagcggagta ccgggcgaag ggaaacaaga tacggataca gtcgaagtgg
     1981 gtccccttgc ggtccatatc gccctacctc atgaaggcgg tcctcatagc ggaggacgac
     2041 aagttctggt cccaccacgg cttcgacacg gaggcgatcc agaaggccct cgagaagaac
     2101 ctggaggcgg gacggttcaa actcggcggc agcacgatca gccagcagct cacgaagaac
     2161 ctctacctca cgccctccaa gaaccccgtc cgcaagctca aggaagcgat catcacctgg
     2221 aggctcgaga gggccctctc aaaacgtagg atcctcgagc tttacctcaa cgtcgtcgaa
     2281 tggggcgagg gtatcttcgg cgccgaggct gccgcgaggc gccacttcgg caagtcagcc
     2341 tcggcgctca ccgcggaaga ggcggccaag ctcgcagcag ccctgcccaa cccaagacgc
     2401 taccgcgtgg acgggacctc ccgctacgtc gaacgccgcg cgaagatcat ctacaccatc
     2461 atggtaagaa ggggcatagt cgtccccgac tacgaagaag tgacaaccac cccgccgga
//
LOCUS       DAOPOA010000330         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_45137_length_2513_cov_2.759967, whole genome shotgun sequence.
ACCESSION   DAOPOA010000330 DAOPOA010000000
VERSION     DAOPOA010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45137_length_2513_cov_2.759967"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKJ17_07415"
     CDS             complement(<1..292)
                     /locus_tag="PKJ17_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOD75837.1"
                     /translation="MTNRAKYIRWFETLGSKDVHLVGGKNASLGEMIHSLKKEGIRVP
                     DGFATTSDAYWSLLDAAHLVEKVEELLDEFERDERSLEKVGRAIRGLFQRSFI"
     gene            complement(320..>2513)
                     /locus_tag="PKJ17_07420"
     CDS             complement(320..>2513)
                     /locus_tag="PKJ17_07420"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOD75838.1"
                     /translation="TAVTLVLVFLAYYIFGLAGQRGASQYMPHWGATRHTGILFVLFL
                     AAWAWGYYLDRYELMFSSRGVVFGPGYADYNVVRIVLWIMLFVSAALGLFTAVSLLLK
                     RFRWILIGVGTCFALMIVLLYMLPFAIQKFVVRPNELELETPFLKHNIAYTRRAYQLD
                     RVEEKRYPALGDLTLKEISQNEDTIRNIRLWDWRPLRQTFRQTQEIRLYYEFYEVDTD
                     RYHLEGEGYRQVMLSARELVGQLPEGARTWINERLQFTHGYGVAMAFVSEQVEGGLPR
                     YVVKDLPPASPYLQIDRPAIYYGEMMPGYRIVNTQIKEFDYPKGDDNVYTNYEGTGGV
                     QLDTFWRRLLFAWDLFDINILISSYITPESRIQIWRRVQERMSRIAPFLRLDKDPYIV
                     IVDGKLYWIQDAYTVSSHFPYSEPYKGGINYIRNSVKIIVDAYNGTVSFYVMDPHDPV
                     LAVYRRLFPNVFQGIDRLPVALKDHLRYPQDLFEAQVDKFQTYHMTDPQVFYNREDLW
                     TLPQEKYAGTPIPVEPYYILMRLPGERTLQYLLMTPLTPRNKDNMIAWMAARCDFPEY
                     GQLLAYQLPKERLIYGPLQIEAMIDQDDVISQQLSLWDQRGSRVIRGNLLVIPVDQSF
                     IYVEPVFLVAEKGDIPQLKRVIIVYGEKVVMEPTIGEAIEAVFGVVRRPAGEVPAAPE
                     TGVIADSARRELERAQNALGRGNWAEFGRAMDRLKEVLARPQDRSKSK"
BASE COUNT          542 a          744 c          694 g          533 t
ORIGIN      
        1 ggatgaaaga tcgctggaag agtccccgga tcgccctgcc caccttttcc agcgatcttt
       61 catccctttc gaactcgtcg agcaactcct ctaccttctc gacaaggtga gcggcatcca
      121 gcaacgacca gtatgcatcg gacgtggtgg cgaacccatc cggcacccgg atgccctcct
      181 ttttcagaga atggatcatc tccccaagag aggcgttctt tccgccgacc aggtgcacat
      241 ctttggaacc cagtgtctca aaccaacgga tgtactttgc gcgattcgtc atgacacctc
      301 cacaggcctg taatggccac tatttacttt tacttctatc ctgcggccgg gcgagcactt
      361 cctttaaccg gtccatggcc cgcccgaact cggcccagtt cccgcggccg agcgcattct
      421 gggctctctc cagttctctc cttgcgctgt cagcgatgac gcccgtctcg ggagcagcgg
      481 gcacttctcc cgcgggtctc cggacgaccc cgaaaacagc ctcgatcgcc tcaccgatcg
      541 ttggctccat gacaaccttt tccccgtaca cgatgatgac ccgtttgagc tgcggaatgt
      601 ctcccttctc cgccaccagg aatacaggtt ccacatagat gaacgactgg tcgacgggga
      661 tcacgagcag gttgccccga atgacccggg agccccgctg gtcccacaga gagagctgct
      721 gcgagatcac gtcgtcctga tcgatcatgg cttcgatctg aagcggtccg tatatgaggc
      781 gttccttggg caactggtag gcaaggagct ggccgtactc ggggaagtca cacctcgccg
      841 ccatccaggc gatcatgtta tccttgttcc gaggggtgag cggggtcatg aggagatact
      901 gcagggtcct ttccccggga agccgcatca ggatataata gggctcgacc gggatcggcg
      961 ttccggcgta cttttcctgc gggagggtcc agaggtcctc cctgttgtag aagacctgag
     1021 gatcggtcat atgatacgtc tgaaacttgt ccacctgcgc ttcgaagagg tcctgcggat
     1081 agcgcaggtg gtctttcagg gcaacgggca gcctgtcgat gccctgaaag acattcggga
     1141 aaaggcgccg gtacaccgcg agcaccggat catgagggtc catcacgtag aacgagacgg
     1201 tcccgttgta agcgtcgaca atgatcttta ctgaattgcg gatatagttt atcccacctt
     1261 tgtagggctc tgaatacggg aagtgtgacg agaccgtgta ggcgtcctgg atccagtaga
     1321 gcttgccatc gacgatgacg atgtagggat ccttgtcaag ccgcaaaaag ggcgcgatcc
     1381 tggacatgcg ctcctgcacg cgcctccaga tctggatcct gctctccggc gtgatgtagg
     1441 aactgatcag gatgtttatg tcgaaaaggt cccaggcgaa taggagccgc ctccaaaaag
     1501 tatcaagctg cacgcctccc gtgccctcgt aattggtgta gacgttgtcg tctcccttcg
     1561 ggtaatcgaa ctcttttatc tgcgtgttga cgatacggta cccgggcatc atctccccgt
     1621 agtagatcgc cggccggtct atctgtaaat agggcgaggc cgggggaaga tccttcacga
     1681 catagcgggg cagtccccct tcgacctgct ctgaaacaaa ggccattgcc acaccgtagc
     1741 cgtgcgtgaa ttgaagccgt tcgttgatcc aggtcctggc cccttcaggc aactggccga
     1801 ccagctcgcg agctgacaac atcacctggc gatacccttc gccttccaga tgatagcggt
     1861 ccgtatccac ctcgtaaaac tcgtagtaaa gacggatctc ctgggtctga cggaaggtct
     1921 ggcggagagg cctccaatcc cacagtctga tgttccgtat ggtgtcctcg ttctgcgata
     1981 tctctttcag ggtgaggtcg ccgagggcgg gataccgttt ctcttcgacc cgatccagtt
     2041 gataggctct gcgcgtatac gcgatgttat gcttcaggaa cggcgtctcg agctccagtt
     2101 cgttgggccg caccacgaac ttctgtatcg cgaagggcag catgtacagc aacacgatca
     2161 tgagggcaaa gcaggtgcct accccgatca atatccatcg aaaccgtttc agcaagaggg
     2221 aaacggcggt gaagagcccc agggccgcgg acacgaagag catgatccag agaacgatcc
     2281 gaaccacgtt gtagtcggca tagcctggtc cgaaaacaac gcctctgctg gaaaacatca
     2341 actcgtatcg gtccaggtaa tagccccatg cccatgctgc cagaaacagt acaaaaagga
     2401 tccccgtatg acgcgtggcg ccccaatgag gcatatactg cgacgctccc ctctgcccgg
     2461 caaggccgaa gatataatag gcaaggaaga cgagcaccag cgtgaccgcg gtc
//
LOCUS       DAOPOA010000331         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_45222_length_2509_cov_3.536675, whole genome shotgun sequence.
ACCESSION   DAOPOA010000331 DAOPOA010000000
VERSION     DAOPOA010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45222_length_2509_cov_3.536675"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..>2509)
                     /locus_tag="PKJ17_07425"
     CDS             complement(11..>2509)
                     /locus_tag="PKJ17_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOD75839.1"
                     /translation="RAFVVPYVNTALANNERVVYMRFARHAPLLEPTGRVTVYKLNAE
                     TGFESFSTQVHNIIRQEGRDVFYVFDCLSDLLLAWATDLMIGNFFTIACPYLYQLNTI
                     AYFALLRESHSFKTVARIRETTQVLIDVYNVDDKIYVHPLKVRGRHSPTMFLPHRKSE
                     DRFLPVTSSVDTGRLLTRISVKGTDLAKRNLDHWDRFFIRIGDLLNENVGSDEQEAVL
                     DQLCRIMIGREERIVALAKKYFTLEDLLAIKARLIGTGYIGGKTVGMLLARKILSRDM
                     SFDWQRVLEPHDSFFIGSDVFYTYLVENGWWKLRMEQKTRDGYFEVAKSLRESMLEGS
                     FPDEIEEQFWRLIEYFGQSPIIIRSSSLLEDAFGNAFAGKYESVFCVNQGTPEERYKQ
                     FGEYVRHVYASTMNEDALAYRLQRGLDKHDEQMALLVQRVSGSYHGSYFYPDLAGVGI
                     SYNTFVWRRDMNPKAGMLRLVAGLGTRAVNRVEHDYPSIVALDLPLLRPHSGPSDMKK
                     YSQHNGDVLNVETNVLETIPVTDIITGDMKADRELMGTRDLEAAEFLRSRGLEPRESW
                     IVTFDRLMSETPLPTIMQRMLKTLETSYQYPVDIEFTINFTASDAFQINLVQCRPLQT
                     KGEGKRVRIPRKVPADRILFQSSGNFMGSNITQGIKRIIVVDPDGYSLLTLSEKYDIA
                     RLIGRINRQIPGQEEMPTILAGPGRWGTTTPSLGVPVRFSEINNIAVLVELAHGTGLG
                     SVMPELSFGTHFFQDLVETGVFYVAIFPDREGSSFNRRIVERKYNLLSMLVPESARYE
                     GIVTVVDTAEDDMKLLADVVTQKVVCFNARKTDQ"
BASE COUNT          457 a          703 c          801 g          548 t
ORIGIN      
        1 gcgacgacgg ttactggtcc gtcttgcggg cgttgaagca gacgaccttc tgagtcacga
       61 cgtcggcgag gagcttcata tcgtcttccg cggtgtcgac gacggttacg atgccttcgt
      121 accgggcgct ctcgggtacg agcatggaaa gaaggttgta tttcctttcc acgatccttc
      181 tgttgaagga cgacccttcc ctgtcgggga agatggcgac gtagaatacc cccgtttcga
      241 cgaggtcctg aaagaagtgg gtgccgaagg aaagctcggg catcacgctg ccgagacccg
      301 tgccgtgcgc cagttccacc aggacggcga tgttgttgat ctcggaaaac cgtaccggga
      361 ctcccagtga gggtgtggtg gtgccccacc gtcccggtcc ggcaaggatc gtgggcatct
      421 cctcctgacc gggtatctgt ctgtttatcc tgccgataag gcgcgcgatg tcatatttct
      481 ccgacagcgt caggaggctg tacccgtcag ggtcgaccac tattatccgc ttgatcccct
      541 gcgttatgtt gcttcccatg aaattccccg aggactggaa gagtatcctg tcggcgggca
      601 ccttgcgcgg gatcctgacc ctctttccct cgcctttcgt ctgcaggggt ctgcactgga
      661 cgaggttgat ctgaaaggcg tcgctggccg tgaagtttat ggtgaattcg atgtccacgg
      721 ggtactggta gcttgtctcc agggtcttga gcattcgctg catgatcgtc gggaggggcg
      781 tttcggacat gagcctgtca aaggtcacga tccatgactc cctgggctca agcccgcggg
      841 acctcaggaa ctcggccgct tcgaggtcgc gtgttcccat gagctcccgg tccgccttca
      901 tgtcgcccgt gattatgtcg gtgacgggga tcgtttcgag aacgttggtc tcgacgttga
      961 ggacatcgcc gttgtgctgg gagtatttct tcatgtcgct cggcccgctg tgagggcgga
     1021 gcagcgggag atcgagggcg acgatgctgg gataatcgtg ctccacgcgg ttcaccgccc
     1081 tggtgccgag gcccgccacg aggcgaagca tccccgcctt cgggttcatg tcccgtctcc
     1141 agacgaaggt gttgtaggat atcccgacac cggcaagatc ggggtagaag tagctgccgt
     1201 ggtaggagcc cgaaaccctc tggaccagga gcgccatctg ctcgtcgtgt ttgtcgaggc
     1261 ccctctggag gcggtaggcc agggcgtcct cgttcatggt gctcgcgtac acgtgccgca
     1321 catactcgcc gaactgcttg tagcgctcct ccggggtgcc ctggttgacg cagaagacgc
     1381 tctcgtactt gccggcaaag gcgttgccga aggcgtcctc gagaaggctg ctcgaccgta
     1441 tgatgatggg cgactgtccg aaatactcga tgaggcgcca gaattgctct tctatctcgt
     1501 cggggaaaga accctcgagc atggattccc tgaggctctt cgcgacctcg aaatacccgt
     1561 cccgggtctt ctgttccatg cgcagcttcc accacccgtt ctcgacgagg tacgtgtaga
     1621 agacatcgga accgatgaag aacgaatcgt ggggttcgag gacgcgctgc cagtcgaagg
     1681 acatgtcacg ggagaggatc ttcctggcga ggagcatgcc gaccgttttc cctccgatat
     1741 agcccgtccc tatgaggcgc gccttgatgg cgaggaggtc ctcgagtgtg aagtacttct
     1801 tcgcgagggc gactatcctt tcctcgcggc cgatcatgat cctgcacagc tggtcgagga
     1861 cggcctcctg ttcatcactg cccacgttct cgttcaggag gtctccgatg cggatgaaga
     1921 acctgtccca gtggtcgagg ttccttttcg ccaggtccgt tcccttgacg gagatgcggg
     1981 tgagcaggcg gcccgtgtcc acgctgctcg tcaccggcag gaaccggtcc tcgctcttgc
     2041 ggtggggaag gaacatcgtg ggcgagtgtc ggccgcggac cttgagagga tgcacgtaga
     2101 tcttgtcgtc gacattgtag acatcgatga gcacctgggt cgtttcgcgg atgcgggcga
     2161 cggtcttgaa ggagtggctc tccctgagca gcgcgaaata ggctatggtg ttgagctggt
     2221 acagataggg gcaggcgatc gtgaagaagt tgccgatcat gaggtccgtg gcccacgcca
     2281 acaggaggtc ggacagacaa tcgaagacat agaaaacatc ccttccctcc tgtcggatga
     2341 tgttgtggac ctgggtagag aaggactcga aacccgtctc ggcgttgagc ttgtagacgg
     2401 tcacccggcc cgtgggttcc agaagagggg catgacgggc aaagcgcata tagaccaccc
     2461 gttcgttgtt ggccagggcg gtgttgacat aggggaccac gaaggcacg
//
LOCUS       DAOPOA010000332         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_45465_length_2498_cov_4.032337, whole genome shotgun sequence.
ACCESSION   DAOPOA010000332 DAOPOA010000000
VERSION     DAOPOA010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45465_length_2498_cov_4.032337"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..833
                     /locus_tag="PKJ17_07430"
     CDS             144..833
                     /locus_tag="PKJ17_07430"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD75840.1"
                     /translation="MWKKRSFLKVLIPVVLAGFLFSCAGAFVSPAARADFEAGLALFN
                     QGKFAEALPYFERAAAAEPDYYEAHLYLGRAYLNMKSFAKAVPPLRTAYRLSPQDFKG
                     QIVDILIDALLGAAFSEVRTGNYEGSLSYLRELMSVEPKAKKVKEDISNVLVAVATSL
                     LMKGDVRDAVKLFGEAVTENPNNAKAYLGLARALLKTGAIPDAIDAGRKALSLDPASN
                     EAFNLMKEMVR"
     gene            complement(1208..1618)
                     /locus_tag="PKJ17_07435"
     CDS             complement(1208..1618)
                     /locus_tag="PKJ17_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75841.1"
                     /translation="MAWWVGVSAAGFSLPLEVYRIGSLKDVQARAASTNIPIVFVYSD
                     ENTRCGLARTATLDVMGELKGSAFVIYVSKSDSDRIPQSVIKAMNSPAAGKFIPKTVA
                     VNPKTGKVEMIIPYISDRNKRIEEIRKTKTLMPR"
     gene            complement(1819..2256)
                     /locus_tag="PKJ17_07440"
     CDS             complement(1819..2256)
                     /locus_tag="PKJ17_07440"
                     /inference="COORDINATES: protein motif:HMM:NF039818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75842.1"
                     /translation="MKIPEYITKAEVQKFCKTLKIRDWTKLKTAKILPKEAKTVLAAV
                     NDTKMSIDPEEFRKGLEVELEHGLTFKDANVTNNHPLVTGKIVIAHLKESLDYYKLLE
                     VAELEGDLHKAIVAGDTKKIKKEYKKLALAKIALAKTEAAQVK"
BASE COUNT          529 a          739 c          700 g          530 t
ORIGIN      
        1 ggactctctg ccgaagatcc agaggatctt tgccaacagc tggcgcggga aatcccagcc
       61 cggctggtgg atacagaacg acggcggagg atgggaaagg aagagataac aaggggtccg
      121 gcgaaagagg gaggtgcatc cagatgtgga agaaaaggtc gttcttgaag gttctcatcc
      181 ccgtagttct ggccggcttt cttttctcct gcgcgggggc cttcgtaagt ccggcggcac
      241 gcgccgactt tgaggccggc cttgccctgt tcaaccaggg gaagttcgcc gaggccctcc
      301 cttactttga gagggcggct gccgctgagc cggactacta cgaggcccac ctctacctcg
      361 gcagggccta tctcaacatg aagagcttcg cgaaggcggt ccctcccctg aggaccgcct
      421 accgcctctc gccgcaggac ttcaagggcc agatcgtcga tatactcatt gacgcgcttc
      481 tcggggcggc cttctcggag gtcaggacgg gcaactacga gggctccctg tcgtacctga
      541 gggagctcat gtccgtggag ccgaaggcga agaaggtcaa ggaggatatc tcgaacgtgc
      601 tcgtcgccgt ggcgacatcg ctactcatga agggcgacgt ccgcgacgcg gtgaagctct
      661 tcggtgaagc cgtcacggag aaccccaaca acgcgaaggc gtacctggga ctcgcgcggg
      721 cgctcctgaa aacgggcgcc atccccgacg cgatagacgc gggccgaaag gccctctccc
      781 tcgacccggc gagcaacgag gccttcaacc tgatgaagga gatggtgagg taaagacggc
      841 ttgaatcgct tgagccgctt gacaggccgt tgaaaaagtc cttttgtacg tcattccggc
      901 gaaggccgga atccaggaaa gatcggctct cggcagaaaa gaggcccggg attcgacaac
      961 atatggtcag cgacaaaggt ctgacatctg tagtctgtag agtaataccg aattggtggt
     1021 ctctcctgga tcccggcctt cgccgggatg acggactgag gctgtgacgg gatgacggcg
     1081 gaacacaaac gggaccggga gccggctttc tcaaaaggct gtcaagcgat tcaagcggct
     1141 cttttcgctt gcccaaaaca agccgctgag agggaaaagg taagggtggt tcactctgcc
     1201 gatgcgatta tctgggcatg agggtcttgg tttttctgat ctcttctatt ctcttgttcc
     1261 tgtcgcttat gtaggggatg atcatttcga ccttgccggt cttcgggttg acggcgacgg
     1321 tcttcggtat gaacttcccc gccgcagggg aattcatggc cttgatgaca gattgaggga
     1381 tgcgatccga atcgctcttt gaaacgtaga taacaaaggc gctgcccttg agctctccca
     1441 tcacatccag cgtggccgtc cgcgcaagtc cgcaccgagt gttctcatcg gaatagacga
     1501 agacgatggg tatgttcgtt gatgcggccc tcgcctgcac atccttcagc gaacctatcc
     1561 tgtacacctc gagaggcagc gaaaaccccg cggccgaaac cccaacccac caagccaaaa
     1621 cgcaactcat cacacagaca atacgcaaca catgcttcat cgcaaacatc ctttgtgaag
     1681 atagtcagtc accttcctcc ggcagacctc taaccaccga aatcccgggc gagccccacc
     1741 ggctgtccgt gtacctgacc agccctcaac cctcaagcca ttcaagcggc tcaagccgtc
     1801 ttcaccctca agccgtcttc acttcacctg cgccgcctcg gtcttcgcca gggctatctt
     1861 cgccagggcg agcttcttat actccttctt gatcttcttc gtatcgcccg ccacgatcgc
     1921 cttgtggagg tcgccttcca attcggccac ctcgaggagc ttgtagtagt cgagggattc
     1981 cttgagatgg gcgatgacga tcttgcccgt gacaagggga tgattgttcg tcacgttcgc
     2041 gtccttgaag gtcagcccat gctcgagctc cacctcgaga cccttgcgga actcctcggg
     2101 gtcgatgctc atcttcgtgt cattgactgc cgcgagcacc gtcttcgctt ccttcggcaa
     2161 tatcttcgca gtcttcagct tcgtccaatc cctgatcttc agggtcttgc aaaacttctg
     2221 aacctccgct ttggtgatat attccggaat cttcattgta accccctcct tgaggatgtg
     2281 gtgatacagg cattgtagca accttcgtgc gattccacaa gatgcgaagg ggagaagacg
     2341 ttatctctcg cccgttcgtc gcttgcgctc ctctctcgag tccgcagaga ccgcaaagag
     2401 aggataaaag agagaaaaga ttgccgccgg atctcggagc ctaccaagat ccggcggcct
     2461 tctcgtcccg ccgtcccggc gggagagaaa agaatatc
//
LOCUS       DAOPOA010000333         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_45494_length_2497_cov_3.024980, whole genome shotgun sequence.
ACCESSION   DAOPOA010000333 DAOPOA010000000
VERSION     DAOPOA010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45494_length_2497_cov_3.024980"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PKJ17_07445"
     CDS             <1..882
                     /locus_tag="PKJ17_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75843.1"
                     /translation="SIAYLQATTVPALIVLRIFHGAVFVLLTSAVIALVVNFIPGEKS
                     GQGFGILSVATMIPYAVIPPLSEALLPRVRNEGNIVSENLGNWDGDYGYEKMSRLDMR
                     EVNDLKIGEGYLIYGEDVRKFKARYIPPANSVKRMSLMKFIRKASRFLHERIKNAIEE
                     TRRHLDKFTAGVYRLYEMFAIGLEEEKIYREYVKARNTLYVENVLPTASSEPYEVFEA
                     MERNRFFHGMEDEARMKSVVERRIKYQKFLDLEWNRFKGSQTQHDMMHVNEETLKFIR
                     DCLIRLDGRTMAAASRK"
     gene            936..1181
                     /locus_tag="PKJ17_07450"
     CDS             936..1181
                     /locus_tag="PKJ17_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75844.1"
                     /translation="MLNATDYTSKLKSLFFSMSRFAAGLYKSLEDLGVTDKSIHLTQE
                     RCWIAMYFILPDDLKLKVDEAVKDALREYEEKKRDHG"
     gene            1341..>2497
                     /locus_tag="PKJ17_07455"
     CDS             1341..>2497
                     /locus_tag="PKJ17_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD75845.1"
                     /translation="METRRTEPSILHFADFHIEDDSWVLKHLEKAANRILSVVDEIKP
                     SLIVIAGDYFEKSLHFDGPGARFAVDFLKKLSGCAPVLMEEGTETHDRESLETLARML
                     PDTVTIVREPSVVSFPYIPELADCDFYCMPGIKKKNFKKVAETYGLDLLKTQPGDLAA
                     YQFQKWAEEKVHREGRDAIFVGHIQLGIDGLDKMKTGYEPAISPETINFIKPSLGLLG
                     HVHDLISYGNLHYSGNIVPRSMTEAESVIVGDDGSVRYTRRPQKKGFYVHTRQPDGSW
                     ENTLHEIETARFCEISIHARDEEDLAVKMQAARDHLKQISTGMDEDGIICKFNIETPD
                     QAVSEALFRHENTVRDEIGGVVDIVSPSINVTSFSDHAARVENIPEWQKKSA"
BASE COUNT          631 a          632 c          713 g          521 t
ORIGIN      
        1 tccatcgcct acctccaggc gaccaccgtc cccgcgctga tcgttcttcg gatcttccac
       61 ggcgcggtct tcgtcctcct cacgtcggcc gtcatcgccc tcgtggtgaa cttcatcccc
      121 ggggagaaaa gcgggcaggg cttcggtatc ctcagcgttg cgacgatgat cccctacgcg
      181 gtgattcccc ccctctccga ggccctcctg ccccgcgtgc ggaacgaggg caacatagtc
      241 tccgagaacc tgggcaactg ggacggcgac tacgggtacg agaagatgtc acgtcttgat
      301 atgcgcgagg tcaacgatct caagatcggc gagggatacc tcatctatgg cgaggatgta
      361 cggaaattca aggcaaggta tattcctccg gcgaactcgg tgaagaggat gagcctcatg
      421 aagttcataa gaaaagcgtc aagatttctc catgagcgca ttaagaacgc catcgaggag
      481 acaaggcgcc atctggacaa gttcaccgcg ggagtttacc ggctctacga gatgttcgcg
      541 atcggtctcg aagaggaaaa gatttacagg gaatatgtga aggcacgcaa tacgctttac
      601 gttgagaatg tgctgccgac agcctcttcg gaaccatacg aggtatttga ggcgatggaa
      661 aggaacaggt tctttcacgg tatggaagat gaagcacgga tgaagagcgt cgtggagagg
      721 cggatcaagt accagaagtt cctggatctt gagtggaaca ggttcaaagg aagccagaca
      781 cagcacgaca tgatgcatgt taacgaggag acactgaagt tcatcagaga ttgcctcata
      841 cgacttgacg gccgtacaat ggccgcggca agccgtaaat agcgggtggg agtgaaatac
      901 acggtaaagg agcctttatg gcaggaaaaa gcgatatgtt gaatgcaacg gattacacga
      961 gtaaactgaa atccctcttt ttctcgatgt cccgttttgc ggcaggatta tacaagtcac
     1021 ttgaagacct gggtgtgacg gataagagca tacatctgac ccaggagaga tgctggatag
     1081 ccatgtattt tatccttccc gatgacctca aactgaaagt ggatgaagcc gtcaaggacg
     1141 ccctgcgcga atacgaagaa aagaagcgcg accacggtta ggcttctgac ggccgggaag
     1201 ctttttcccg cgggatgatt gatatgtctg ttgcgcgccc cgtgcctggc aaggcccggg
     1261 gtttcttttt tctctgctct ccgcttggcg tgtgatgcta tttccatgtg taatgctaca
     1321 cggaggaggc accccagaac atggaaacga gaaggaccga accatccata cttcacttcg
     1381 ccgattttca catcgaggac gacagctggg tgctcaaaca cctggaaaag gcagccaacc
     1441 gcatcctgtc cgtcgttgac gagataaaac cgtcccttat cgtcatagcc ggtgactatt
     1501 ttgagaagag cctccacttt gatggtcccg gtgcccggtt cgcggtcgac tttctgaaaa
     1561 aactgagcgg ctgtgcgccg gtcctcatgg aggaaggcac cgaaacacac gacagagagt
     1621 cgctcgaaac cctggcgagg atgctgcccg acaccgtcac catcgtgcgg gagccgtcgg
     1681 ttgtgtcatt cccttacata cccgagctcg ccgactgcga cttctattgc atgcccggga
     1741 taaagaagaa gaacttcaag aaagtggcgg agacatacgg tctcgatctc ctaaagacac
     1801 agcccgggga tctcgcggcc tatcagtttc aaaaatgggc ggaagagaag gtccatagag
     1861 aaggcaggga tgccatattc gtgggccata ttcagctggg tattgacggt ctcgacaaga
     1921 tgaaaacggg atatgagccg gcgatatcac ctgagacgat caatttcatc aaaccgtccc
     1981 ttggtcttct tggtcacgtg catgatctca tcagctatgg caacctccac tattctggga
     2041 atattgtgcc gagaagcatg accgaggcgg agtccgtgat cgtaggagac gacggcagtg
     2101 tacggtacac gcggcggccc cagaaaaaag gcttctatgt gcacactcgg cagcccgatg
     2161 gttcgtggga aaatactttg catgaaatcg aaacggcgag gttctgtgag atttccattc
     2221 atgcccgcga tgaggaggat ctcgccgtga agatgcaggc ggcccgggat cacctgaaac
     2281 agataagtac ggggatggac gaggacggca ttatctgcaa gtttaacatc gagacccccg
     2341 accaggcggt gagcgaagca ttgttccgtc acgaaaacac ggtgagggat gagatcgggg
     2401 gtgtcgtcga tatcgtgtcc ccctctatca acgtgacatc attctcggat catgcggccc
     2461 gggtggagaa tataccggaa tggcagaaaa agagcgc
//
LOCUS       DAOPOA010000334         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_45577_length_2493_cov_3.359721, whole genome shotgun sequence.
ACCESSION   DAOPOA010000334 DAOPOA010000000
VERSION     DAOPOA010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45577_length_2493_cov_3.359721"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKJ17_07460"
     CDS             <1..585
                     /locus_tag="PKJ17_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75846.1"
                     /translation="ANVTDMMLAKREMESRMRKLNMVIGVFFSEVGIGLLETFSRYDP
                     EFDGIRPSLLATANWTDKDFDRAIRNSRTQEYGAKLAHIDFASLKILLMDRRDFLVRL
                     LENPVLLEHESFTGLLRAVFHLTEELAYRSGFDDIPQSDASHLAGDIKRAYHLIVGEW
                     LVYMKFLKKNYPFLYSLALRTNPFDAQRSVTVKA"
     gene            complement(1051..1554)
                     /locus_tag="PKJ17_07465"
     CDS             complement(1051..1554)
                     /locus_tag="PKJ17_07465"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOD75847.1"
                     /translation="MKKFPLRRWCGVSGTAGFDIPALSCDIILPMTDPDFLPVTAAVI
                     EKDGKILIARRKHPYPFPGPPWEFPGGKLEDGETLEECLRREIREELGIDIAVGRLIS
                     SRKHVLNCQSAIMLYAYWAHHLSGDIVLTDHEEVAWVAIEDLEVYSFPNPDRDIVKDI
                     LKTGMTG"
     gene            1770..2012
                     /locus_tag="PKJ17_07470"
     CDS             1770..2012
                     /locus_tag="PKJ17_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75848.1"
                     /translation="MNELVQARGNPDRYETDGKGNRVLVYEWTWESTYETPGRAWRDA
                     SGLRWTNPKTETILHREKRAYYVNKQGIVFDGKWAW"
     gene            complement(2134..>2493)
                     /locus_tag="PKJ17_07475"
     CDS             complement(2134..>2493)
                     /locus_tag="PKJ17_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75849.1"
                     /translation="RWGIKRSFIIILCCQCLTMLLPFLGSSQWQLWAFVVIFGFGYGG
                     LASIFPLAHAELFGVKAMGSLFGISLLGATIGGSAGPLIAGFSFDMTRSYFTAFGAGA
                     ISIAIAILLATGIKKKA"
BASE COUNT          546 a          705 c          739 g          503 t
ORIGIN      
        1 gccaacgtga cggatatgat gctcgcgaaa cgggagatgg agagccggat gcggaagctc
       61 aacatggtga tcggggtctt tttcagcgag gtcggcatag ggctcctgga gaccttttcc
      121 cggtacgacc ccgagttcga cggcattcgc cccagtcttc tggcgaccgc gaactggacg
      181 gacaaggact tcgacagggc gatcaggaat tcgcgcaccc aggaatatgg agcaaagctt
      241 gcgcacatcg atttcgcttc gctgaagatc ttgcttatgg acaggcgtga tttcctcgtg
      301 cgtctcctcg agaaccccgt cctcctggag cacgaatcct tcacgggcct tctgcgcgcc
      361 gtctttcacc tgacagagga actggcctac cgtagcggat tcgatgatat tccgcaatcc
      421 gacgccagcc atctcgcggg cgacatcaag cgcgcctatc atctcattgt gggcgagtgg
      481 ctcgtctaca tgaaattcct caagaagaac taccccttcc tctactccct ggccctgcgc
      541 accaatccct tcgatgcaca gaggtcggtg accgtaaagg cgtagacctg cccgcaagaa
      601 cggtcggccc gggtatggtc acacgggaat atacatcccc gcggaccgcc gagatcggtc
      661 cccaccgacg gccggatgca ctctctatcc tgtcttcttc tcgcgctctc cccgctctcc
      721 gtcgggcccg ccgtgatgtc gaggcaaggc cgcagtggtt gaaccggcgc ccacctgaat
      781 ggttttcggt taagcctgcg aagagacggg ggagggatta cttcttcttg gagcttttct
      841 ggttgaagat gagcagtccc acgacgaacc cgaacataac gatacccgtg atggccatga
      901 tggtattgaa cataacaacc cccttagttt tgctaacatc agactaacac caggcgaggg
      961 tggttaccgt agtccacgct acatagcagg catatttttt tgggggatct tccacggggg
     1021 tgaggtcgcg ttcctttgtc cggccgttgg ctagccggtc atccctgtct tcaggatgtc
     1081 cttgacgatg tcccggtcgg ggtttgggaa ggaatatacc tcaagatctt caatggcgac
     1141 ccatgcgacc tcctcgtggt cggtaaggac gatgtcgccg gagagatgat gggcccaata
     1201 ggcatagagc atgatggccg actggcagtt caggacatgc tttctcgacg atatgagcct
     1261 gcccacggct atgtcgatac cgagttcctc ccgtatctcg cgcctcaggc actcttcaag
     1321 ggtttcgcca tcctcgagct tgccgccggg gaactcccag ggaggccccg ggaagggata
     1381 ggggtgtttc cggcgggcta tgagtatctt gccgtccttc tcaataacgg cggcggtgac
     1441 aggcaggaaa tcggggtcgg tcatcggaag aataatatcg cacgagagcg ccgggatgtc
     1501 aaaacctgcc gtgcctgata cgccacacca ccgccgcaaa gggaactttt tcatccttac
     1561 cccgtcatat tggggagaaa ggagataaca tgatagcgac gcaaataccc ttggcccctt
     1621 cacctatggt cgaaccaatg acaaagtgcg tctcgtgctg gctatggcgg ttctttatcg
     1681 ttgcagcctt gggttacctg ttccttacgc tgggaggttg cggggttcta agcaggaacg
     1741 ccgagataag tgcctggatc ggacgcccca tgaacgaact tgttcaggcc cgcggcaacc
     1801 cggaccggta cgagaccgac ggcaaaggca acagggtcct cgtctatgaa tggacatggg
     1861 aatccaccta tgagaccccc ggccgcgcct ggcgcgacgc aagcggtctg cgctggacta
     1921 atcccaaaac ggagaccatc ctccacaggg aaaaaagagc gtactacgta aacaagcagg
     1981 gcatcgtctt cgacgggaag tgggcgtggt aaagccagct tgagtggctt gaatagcttg
     2041 aatagctcga aggccaagaa ataaagagac ctttccttct ctcttcagag gatgcgcctt
     2101 tgaccctgaa gccactcaag cgattcaagc tgttcaagcc ttcttcttta tccccgtggc
     2161 gaggaggatg gctatggcta tggagatcgc cccggcgccg aaggccgtga agtaggaacg
     2221 ggtcatgtcg aaggagaaac cggcgatgag ggggccggcc gagccgccta tggtggcgcc
     2281 caggagcgag atgccgaaga gggagcccat ggccttcacc ccgaagagct cggcgtgggc
     2341 aagggggaag atggaggcga ggccgccgta cccgaacccg aagatgacga cgaatgccca
     2401 gagctgccac tgggagctcc ccaggaaggg aaggagcatt gtgaggcact ggcagcagag
     2461 gattatgatg aaggagcgct ttatccccca ccg
//
LOCUS       DAOPOA010000335         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_45580_length_2493_cov_3.317473, whole genome shotgun sequence.
ACCESSION   DAOPOA010000335 DAOPOA010000000
VERSION     DAOPOA010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45580_length_2493_cov_3.317473"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="PKJ17_07480"
     CDS             complement(<1..1579)
                     /locus_tag="PKJ17_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOD75850.1"
                     /translation="MNQAELIGKIIEISHSNLELTARINAILNIIAQDLGFDEAIVYT
                     FDKDKRLTCRYANQKSMLFRILSRYRCHIGEGIVGSVAQRRSPQFFTFKDISPRFGCL
                     FYPELDDHLERFRTFSFLPLSDDSYLFGVVVLCSLMRDSLQEQEKVLLSVISRELGGI
                     LRAFELILSSKKRISELAMLSELGKILTSNVGPYELLKSIALIIAKSLSASFVSVKLE
                     YPLLKLETQRFTYGMIDPAAKEYVEQLERSAIKLLRPVSLRNGTGEQLDNPPPFSLYS
                     APILSKNRVLGTITLCGEKSDQHGIEENGQYIINTISNYISSGLENTLLNTRLRNVVR
                     ELTDAQKRLIEQEKFRSLGEMTANIAHEIKNPLVIIGGFTKRLAKKMQNDRTENRYID
                     IILNEVTRLETILNEVLHYVKENQPYEEFCRLQDFLEDILYIVSSDPAWESVTIIKDL
                     DVSLEPVPCDSQQLKQVFINLLMNAFEAMRGAGSVTVKTRKVMYDEQPFVAVSITDTG
                     GGIDPAIIDNIFNPFFTT"
     gene            complement(1554..>2493)
                     /locus_tag="PKJ17_07485"
     CDS             complement(1554..>2493)
                     /locus_tag="PKJ17_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOD75851.1"
                     /translation="HYDMHLLNIGWEYFTPESLEFFGHINFLKGGIIFSDILNTVSKQ
                     YSREIQTPEFGCGLDGILTKRRDDLYGIVNGIDYGDWNPGTDTSLPGRYTIENRANKA
                     MCKKALQQVFDLPADPKAPLLATISRLADQKGFDLISEAMEKIHGLGVQYVILGTGER
                     KYHDLFTTLAKKFPRSFSIKIAYDNKLAHLMEAGADMFLMPSRYEPCGLNQLYSLKYG
                     TVPVVRGVGGLEDTIIDFTDSPESGTGFKFYEYTKEAMLDAIGRALSLYGDKKAWEDL
                     VTRCMKEDFSWEKSAREYAELYRKAIAKHESSGTDR"
BASE COUNT          488 a          656 c          752 g          597 t
ORIGIN      
        1 tcgtcgtgaa gaaggggttg aatatgttat cgatgatggc ggggtcgatg ccgccccccg
       61 tgtctgttat cgaaaccgcg acgaagggct gttcgtcgta catgaccttc cgggtcttta
      121 ccgttacgct gccggcgccc ctcatggcct cgaaggcgtt cataaggaga ttgatgaaga
      181 cctgcttgag ctgttgactg tcgcagggta cgggctccag cgagacatcg aggtccttga
      241 tgatggtgac cgattcccag gccgggtcgg aagaaacgat atacaggatg tcctccagga
      301 agtcctggag cctgcagaac tcctcgtacg gctggttctc cttgacgtaa tggagcactt
      361 cgttaaggat ggtctccagg cgcgtgacct cgttgaggat gatatcgata tagcggttct
      421 ccgtgcggtc gttctgcatc ttcttggcca gccgcttggt gaagccgccg atgatgacga
      481 gcgggttctt gatctcatgg gctatattgg ccgtcatttc gcccaggctg cggaacttct
      541 cctgttctat gagcctcttc tgggcgtccg tcaactccct gaccacgttt ctcaggcgtg
      601 tattgagcaa cgtgttctcc agtccgctcg atatatagtt cgatatggtg ttgattatgt
      661 actgtccgtt ctcttcgata ccgtgctggt cggacttctc gccgcagagg gtgatggtgc
      721 ccaggaccct gttcttcgat agaatgggtg cagaataaag ggagaagggc ggagggttgt
      781 cgagttgctc cccggtcccg tttctcaaag aaacgggacg caggagtttt atggccgacc
      841 gttccagctg ctccacgtac tccttcgcgg cggggtctat catgccatag gtgaaccgct
      901 gcgtttccag tttgagaagg gggtattcca gcttgacgga cacgaaggag gcggagagcg
      961 acttcgcgat gatgagcgct atgcttttta agagctcgta gggccccacg ttggatgtca
     1021 gtatcttgcc gagttccgac agcatcgcca gctcgctgat gcgttttttc gatgagagga
     1081 tcagttcgaa ggccctgagg ataccgccga gctcgcggga tatgacggag aggaggacct
     1141 tctcctgttc ctgcagcgaa tcgcgcatga gcgaacacag gacgacaacc ccgaagaggt
     1201 agctgtcgtc ggacagggga aggaaggaaa aggtcctgaa cctttccagg tgatcgtcga
     1261 gctcgggata gaaaaggcag ccgaacctcg gggatatatc cttgaaggta aagaactgcg
     1321 gagaccgcct ctgcgccacg ctgccgacga tcccttcacc gatgtggcag cggtagcggc
     1381 tcaatatcct gaaaagcatg cttttctggt tggcgtagcg gcaggtcagc ctcttgtcct
     1441 tgtcgaaggt atagacaatg gcctcgtcga acccgaggtc ctgggcgatt atgttgagga
     1501 tcgcgttgat ccgcgccgtc agctcgaggt tcgagtgcga gatctcaatt atcttaccga
     1561 tcagttccgc ttgattcatg cttcgcaatc gctttcctgt aaagttccgc gtactcgcgg
     1621 gcagattttt cccaggaaaa gtcttccttc atgcagcggg tcacgaggtc ctcccaggct
     1681 ttcttgtccc cgtacaggga aagcgccctg ccgatggcat cgagcattgc ctctttcgtg
     1741 tattcgtaga acttgaagcc cgttccgctt tcgggagagt cggtgaagtc gatgatcgtg
     1801 tcctccagac cgcccacgcc tctgacaacg gggacggtgc cgtacttcag gctgtaaagc
     1861 tggttgaggc cgcacggttc gtaccgggaa ggcatgagga acatgtccgc ccccgcctcc
     1921 atgaggtgcg ccagcttgtt gtcgtaggct atctttatgg aaaaggatct ggggaacttc
     1981 ttcgcaaggg tggtgaagag atcatgatac ttccgttccc ccgtgcccag aatgacgtac
     2041 tgcacaccga ggccgtggat cttttccatg gcctccgaga taaggtcaaa acctttctgg
     2101 tcggcgagac gcgaaatggt cgccagaagc ggggccttcg ggtcggcagg cagatcgaag
     2161 acctgctgga gggccttctt gcacatggcc ttgttcgccc ggttctcgat ggtgtaccgc
     2221 ccgggcaggg acgtgtccgt cccggggttc cagtccccgt aatcgatacc attgacgatg
     2281 ccgtagaggt cgtcccgccg cttggtgaga attccgtcga ggccgcatcc gaactccggg
     2341 gtctggatct ccctgctgta ctgcttgctt acggtgttca gaatgtcgga aaagatgatg
     2401 ccgcccttga ggaaattgat atggccgaag aactccaggg attccggggt gaaatactcc
     2461 cagccgatgt tgaggaggtg catgtcgtaa tgc
//
LOCUS       DAOPOA010000336         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_45905_length_2480_cov_3.621856, whole genome shotgun sequence.
ACCESSION   DAOPOA010000336 DAOPOA010000000
VERSION     DAOPOA010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45905_length_2480_cov_3.621856"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2263)
                     /locus_tag="PKJ17_07490"
     CDS             complement(<1..2263)
                     /locus_tag="PKJ17_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD75852.1"
                     /translation="MLSKFFLDRPVFAWVIAIVIMLVGALAIHSLPISQYPPIAPPSI
                     YIASSYPGASAETVENSVTQIIEQKLTGLDNMLYLSSTSDSAGGSRITLTFAPGTDPD
                     LAWAKVQNKLQLAMTSLPETVQRQGVTVGKATRNWLMIVNLISEDGSMDGDDLRDYAQ
                     SNLEKVLARIPGVGEVENFGSQYAMRIWLNPDRLTDFSLSMADVTAALKTYNVEVSAG
                     QFGGAPAVKGQRLNTSIIVQSMLRTPEEFSSIPIRANPDGSTVRVGDIGRVELGTDAY
                     DIEAFYNGSPSAGIGIRMASGANALDTADAIKAKLEEMSRFFPPGMKVVYPYDTTPFV
                     KVAIGEVVETLLEAILLVFLVMWLFMGNIRATLIPTIAVPVVILGTFAVLEFFGFSIN
                     MLTMFAMVLSIGLLVDDAIVVVENVERIMSEEGLPPKEATAKSMTQITSALIGIGLVL
                     SAVFGPMAFFGGSTGVIYRQFSVTIIASMLLSVVVALILTPVLCATFLKPVPKGHEPA
                     ENAWRLLRPFFAWFDRRFFRLRDRYVGIVERSFTRRKRYFVAYFVIAGIAGLILWNMP
                     TSYIPDEDQGIMLAQVMMPTGSTLEQTKEVVNRLQRYFQENEKDAVESTMTISGIGFS
                     GRSQTNGMVFVKLKDWNKRGSARLKVKAVAQRATREFSALRGALVFAFPPPPVIELGM
                     ATGFDFMLLDRGGLGHQKLMEARNQLLGIMSKDKRVVKVRPNGMEDIPEYRVDIDWQK
                     AGALGVPISSIHNT"
     gene            complement(2273..>2480)
                     /locus_tag="PKJ17_07495"
     CDS             complement(2273..>2480)
                     /locus_tag="PKJ17_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75853.1"
                     /translation="ITTERAIGDRWLVSAGLKSGDRMIVEGLQKVRPGVPVKAVPFKT
                     AGNDDKATADTGGQKPASPSPKAK"
BASE COUNT          502 a          692 c          776 g          510 t
ORIGIN      
        1 tcgtattgtg gatggagctg atgggtacac cgagggcccc ggccttttgc cagtctatgt
       61 cgacacgata ttcggggatg tcctccatgc cgttgggcct caccttcacc acgcgcttgt
      121 cctttgacat tattccgagg agttgatttc gtgcctccat cagtttctgg tggccgagac
      181 ccccgcggtc gaggagcatg aagtcgaaac cggtagccat gccgagttcg atgaccggag
      241 gcggaggaaa ggcaaaaaca agggcgccgc gcagggcaga gaactccctc gtggcccgct
      301 gggcaacagc cttgaccttg agacgcgcgc tgcctcgttt gttccagtcc ttgagcttga
      361 cgaaaaccat gccgtttgtc tgtgaccgcc cggagaagcc tatacccgag atggtcatcg
      421 tcgattcaac ggcgtccttt tcgttttcct ggaaatatcg ttgaagccgg ttgacgacct
      481 ccttggtctg ctccagggtc gaccccgtgg gcatcatcac ctgtgccagc atgatcccct
      541 gatcctcgtc ggggatatag gacgtcggca tattccacag gatcagtccc gcgatgccgg
      601 cgatgacgaa ataggcgacg aaataacgtt ttcttctcgt gaaggagcgt tcaactatcc
      661 ccacgtacct gtccctcaac ctgaagaagc gccgatcgaa ccaggcaaag aacggcctca
      721 ggagacgcca tgcgttctcg gccggctcat ggcccttcgg taccggtttg agaaaggtgg
      781 cgcaaaggac gggtgtcagt atgagagcca cgacgacgga caggagcatg gaagcgatga
      841 tggtcacgga gaactggcgg tagatgaccc ccgtggaacc accgaagaag gccatggggc
      901 cgaagacggc ggaaagaacg agtccgatcc cgataagggc gctcgtgatc tgtgtcatgg
      961 acttcgcagt ggcttccttg gggggaagcc cttcctcact catgatacgc tccacgttct
     1021 cgacgacgac gatggcgtcg tcgacgagaa gcccgatgga gaggaccatg gcgaacatcg
     1081 taagcatgtt gatggagaat ccgaagaatt cgagtaccgc gaaggtgccg aggatgacaa
     1141 cgggcaccgc gatggtcggg atgagggtgg cccgtatatt gcccatgaag agccacatga
     1201 cgaggaagac gagaaggatc gcctcgagga gggtctccac gacctcgccg atggccacct
     1261 tcacaaaagg agtggtatcg taagggtaga cgaccttcat gcccggggga aagaaacggc
     1321 tcatttcctc gagttttgcc tttatcgcat cggccgtatc gagggcattc gcacccgagg
     1381 ccatccgtat cccgatgcca gccgacggac tgccgttgta aaacgcctct atgtcgtagg
     1441 catcggtgcc gagctccacg cggcctatgt ccccgacccg gaccgttgag ccgtcggggt
     1501 tggcgcggat gggaatggag ctgaactcct cgggcgtccg gagcatgctt tggacgatga
     1561 tggatgtatt gaggcgctgg cctttgacag cgggtgctcc gccgaattgg cccgccgaga
     1621 cctccacgtt atacgtcttc agcgccgcgg tgacatccgc catggagagg ctgaaatccg
     1681 tgagacggtc aggattgagc cagatgcgca tggcatattg agaaccaaaa ttttccactt
     1741 caccgacgcc ggggatccgc gcgaggacct tttccaggtt cgattgcgcg taatcccgca
     1801 ggtcatcacc gtccatgctg ccatcttccg atatgagatt gacgatcatg agccagttcc
     1861 tggtggcttt gccgacggtg acaccctggc gctggacggt ctcgggaagg ctcgtcatgg
     1921 ccagttggag cttgttctgc acctttgccc aggcaaggtc ggggtcggtg ccgggcgcga
     1981 aggtgagggt aatgcgggaa cctcccgccg aatcactcgt ggaggacagg tagagcatgt
     2041 tgtcgaggcc cgtcagtttt tgttcgatga tctgggtgac actgttttcg acggtctccg
     2101 ccgacgctcc cgggtagctg ctcgctatgt agatcgaggg aggggcgatg ggcgggtact
     2161 gggatatggg caggctgtgt atcgccaggg cgccgacaag catgataaca atagctatga
     2221 cccaggcgaa gacagggcgg tcgaggaaga acttggataa catcgggccc tcctactttg
     2281 cctttgggct cggagaggcc ggtttctgac cgcccgtgtc cgccgtcgcc ttgtcatcat
     2341 tgccggcggt cttgaagggg accgccttca cgggaactcc cggacggacc ttttgaagtc
     2401 cctcgacgat catgcggtcg ccgctcttga gtcccgctga aacgagccac ctgtctccga
     2461 tagcgcgctc ggtggtgatc
//
LOCUS       DAOPOA010000337         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46023_length_2476_cov_3.780256, whole genome shotgun sequence.
ACCESSION   DAOPOA010000337 DAOPOA010000000
VERSION     DAOPOA010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46023_length_2476_cov_3.780256"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..691)
                     /locus_tag="PKJ17_07500"
     CDS             complement(230..691)
                     /locus_tag="PKJ17_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOD75854.1"
                     /translation="MRLTDILKESSIISDIKGTTKEEILTELAGQLKAAGLVKDVDSV
                     VRIIMEREALGSTGIGDGVAIPHGKMKGPGGVLVAFGRSRQGVDFDAVDEQPVHIFFL
                     VLAPEDSASLHLKVLSRISKILRDPSFRKKLLKLPDAHSLYESIVEEDGKL"
     gene            complement(688..1212)
                     /gene="raiA"
                     /locus_tag="PKJ17_07505"
     CDS             complement(688..1212)
                     /gene="raiA"
                     /locus_tag="PKJ17_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOD75855.1"
                     /translation="MDITTSFRHVDTSEDVRRYVEEKLSRLQKYVETPLDIHVVISLE
                     RKYRQRIDVMFTINGVVINAHEVMDDIYAAIDSILDKLERRLTKYRDKMKEYRESKLK
                     RTTPPPTSEESGIIVSRTVEAKPMDPEEAVMQLTASGDMFMVFRERERDNVCIVYKRK
                     DGKFSLIETAGKGP"
     gene            complement(1376..>2476)
                     /gene="rpoN"
                     /locus_tag="PKJ17_07510"
     CDS             complement(1376..>2476)
                     /gene="rpoN"
                     /locus_tag="PKJ17_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOD75856.1"
                     /translation="PDYENMVRKTYNLRDHLRWQIGLSDFSPRERVVAEWIIENIDDN
                     GYLICGLEEISRDSGFPVEVLEPVLRKVQRLEPAGVGARDLKECILIQYEVSGEKDPV
                     FENVVANHFELFQHNNLRGIAKATGFPIERIKEVFEKIKSFDPKPGRNFSDEFISYVV
                     PDVYVVKGEEGFDIALNNDDVPELRMSRYYLELATDRKVSGETKRYIKNKMKQAEWFI
                     KSIQQRQRTLFLVAQSIVNFQREFFENGLRSLRPLILKDVAQDVGVHESTVSRITTSK
                     YMSTPHGVYEMKFFFPTSINKGEGDPLSTNVVMDLIAELVKKEDKKKPLTDDEIAHIL
                     KSRHDINIARRTVAKYREILNIRSSRERTVID"
BASE COUNT          454 a          733 c          659 g          630 t
ORIGIN      
        1 gtggggatgg agatagacca tatggcccgt tatgacgagg ccgagcttct ggatcctcgg
       61 gttgtagatc ttacgggcga gccccttttt gccggccaga acctcaagtt ccaatccgta
      121 tagctcgtca tctacaagct gttgaacctc tattcccttc atgtgccttt ccgcgaggtc
      181 ctcccccgga gggaggcatg cttcccggcc cgtccctcga ggcgggccct tacagctttc
      241 cgtcctcttc cacgatgctc tcatacaggg aatgggcatc ggggagcttc agcagcttct
      301 ttctgaacga agggtcgcga aggatcttcg atatccgcga caggaccttc aaatgcaggc
      361 ttgccgaatc ctcgggggcg aggacgagaa agaagatgtg caccggctgc tcgtcgacgg
      421 catcgaaatc gacaccctga cgtgaccggc cgaatgcgac gaggaccccg ccgggaccct
      481 tcatcttgcc atggggaatg gcgacgccgt cgcctatgcc cgtgctcccc agggcctccc
      541 gttccatgat gatcctcacg acggagtcaa cgtcctttac aaggcccgca gccttgagct
      601 gccccgcaag ctcggtgagt atctcctcct tggtggtgcc cttgatatcg gagatgatcg
      661 aggattcttt cagtatgtcg gttaacctca cggtcccttc cctgccgttt ctatgagact
      721 gaacttcccg tccttccgtt tgtaaacgat gcagacgttg tcgcgttccc tctcgcggaa
      781 gaccatgaac atgtcccccg aggccgtaag ctgcatcacg gcctcctcgg ggtccatggg
      841 tttggcttcc acggtcctcg atacaatgat gcccgactcc tcgctcgtag gcggcggtgt
      901 ggtgcgtttc agcttgctct cgcggtactc cttcatcttg tcgcggtact ttgtaagtct
      961 tcgttccagc ttgtcgagta tgctgtcgat cgccgcatag atatcatcca tgacctcatg
     1021 ggcattaatg acgaccccgt tgatggtgaa catgacatcg atacgctggc ggtatttccg
     1081 ctcgagggag atgaccacgt ggatgtcgag gggcgtctcc acgtatttct gcagacggga
     1141 gagcttctcc tcgacataac gccttacatc ttcgctggta tcaacgtgcc tgaagctggt
     1201 tgtgatatcc atagttcctc cgcttacaaa aataaggttg gcgtggacca accttggagc
     1261 ttattaataa cagatatgcc ggatcgtgtc aatccaattc cccgaaagta ttacgttaat
     1321 cgatcaggag cgtaaacgct ccgccaaggc ttccccgtat ccggaatctg tgcctttagt
     1381 ctatcaccgt cctttcccgt gaggaccgaa tgttgagaat ctccctgtat ttcgcgaccg
     1441 tacggcgggc tatgtttatg tcatggcgcg atttcaggat gtgggctatc tcatcgtccg
     1501 tgaggggctt cttcttgtct tccttcttga cgagctccgc tatgaggtcc atgacgacat
     1561 tcgtcgaaag agggtcccct tctcccttgt tgatgctcgt cgggaagaag aacttcatct
     1621 cgtacacacc gtggggggtg ctcatgtact tgctcgtcgt tatgcggctt accgtcgatt
     1681 cgtggacccc cacatcctgg gcgacgtcct tgaggataag gggccttaag cttctcaggc
     1741 cattctcgaa gaactcacgc tggaagttca cgatgctctg ggcgacgaga aagagcgtcc
     1801 tttgcctctg ctggatactc ttgatgaacc actccgcctg cttcatcttg ttctttatgt
     1861 acctcttcgt ctcgccgctc accttcctgt ccgtcgcgag ctccaggtag tagcggctca
     1921 tcctgagctc cgggacatcg tcgttgttga gggcgatgtc gaacccttcc tcgcccttga
     1981 ccacatagac gtcggggacg acataggata tgaactcgtc gctgaagttc cttcccggtt
     2041 tgggatcgaa ggacttgatc ttctcgaaga cctccttgat cctctctatg gggaaaccgg
     2101 tcgcctttgc tatccccctg aggttgttgt gctggaagag ttcgaagtga ttggcgacga
     2161 cgttctcgaa gacggggtcc ttctcacccg acacctcgta ctgtatgagg atgcattcct
     2221 tcaggtcccg cgcgcccacc ccggccggct cgagcctctg cacctttctg aggaccggct
     2281 cgaggacctc gacgggaaaa ccggaatccc gcgatatctc ctcgaggccg cagatgaggt
     2341 acccgttgtc gtcgatgttc tcgatgatcc attccgccac cacccgttcc cgtgggctga
     2401 agtccgaaag gccgatctgc cacctcaggt ggtccctgag gttgtacgtc ttcctgacca
     2461 tgttctcgta gtcggg
//
LOCUS       DAOPOA010000338         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46027_length_2476_cov_3.397356, whole genome shotgun sequence.
ACCESSION   DAOPOA010000338 DAOPOA010000000
VERSION     DAOPOA010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46027_length_2476_cov_3.397356"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PKJ17_07515"
     CDS             complement(<1..321)
                     /locus_tag="PKJ17_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOD75857.1"
                     /translation="MYSYTATTLEGAIVEGVVEASDERTAVEKIRNTGVIPIKVSLPR
                     EGLGKTFAMRSSRGDLLTFTTELSALLGAGLPIDRSLNILSEISEGKQMKEVINSVLK
                     GIREG"
     gene            complement(328..1857)
                     /gene="gspE"
                     /locus_tag="PKJ17_07520"
     CDS             complement(328..1857)
                     /gene="gspE"
                     /locus_tag="PKJ17_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOD75858.1"
                     /translation="MSKESAIFLDAEDFPKVPLVIDGISTRFIRENRIIPFEFKNDVL
                     KVIMADPDDREVIEALRVAAAGEVIVYTAAREVIDEHLSKFYSQEEQSINRIMEGMDE
                     ESQELFNEPEEEDIGHLKDLASEAPIIKLVNLLITRAVESRASDIHIEPFGDEVKVRY
                     RIDGVLSDVESAPKRLQAAMVSRIKIMAKLNIAERRLPQDGRIRLLVGDREIDLRVST
                     IPILHGESIVMRILDKESIVIDLDALGFMPDTLNNFNRLIKRPNGIILVTGPTGSGKT
                     TTLYGALDKINSPDKKIITVEDPIEYQLKGVNQIQVRPNIGLSFTNTLRHIVRQDPDV
                     VMIGEIRDLETAEIAIQAALTGHLVFSTLHTNDAPSAITRLLDMGVESFLLSSTVRGI
                     LAQRLVRVICLTCKAEDTSTDEAQELEALGIRGDARLYRGRGCEACTYTGFRGRMGIF
                     ELLLVDEVMRRLILRKADEGEIRDAARSGGMKTLLEDGIRKIEAGSTTLDEVFRVTQE
                     D"
     gene            complement(1881..>2476)
                     /locus_tag="PKJ17_07525"
     CDS             complement(1881..>2476)
                     /locus_tag="PKJ17_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75859.1"
                     /translation="FGSGTVAPQRTIQYKDIGIILKVKPRINEGGLVTLDITQEVSSY
                     ETIKLFDNEDYIILKKTETTTSLVVQDGNTIIIGGLIRDDISKSRSGIPLLSKIPILG
                     YLFGQTTQSDQRTELIILLTPRVIRTHADANAVTSDYVDSVTRTGKGKVKREELIKIK
                     PVPPPPAAPQDGFPRDGAERKGGAAQDGFQTQPERVE"
BASE COUNT          399 a          750 c          730 g          597 t
ORIGIN      
        1 tccttcgcgg atacctttga gcacggaatt gatgacctcc ttcatttgct tcccttccga
       61 tatctccgag agtatattga ggcttctgtc gatgggaagg ccggcaccca gcagcgccga
      121 cagctccgtg gtgaacgtga ggaggtcacc ccgggacgat ctcatggcaa aggtctttcc
      181 gagaccctcc cggggtagcg acaccttaat tggtatgacc ccggtgttcc ttattttctc
      241 aacggcggtc ctttcgtccg acgcctcgac gacgccttcg acgatcgcac cctctagggt
      301 cgtggcggta tatgaataca tggccaccta atcctcctgg gtgaccctga agacctcgtc
      361 cagggtcgtg ctgcccgcct cgatctttcg tatgccatct tcgaggaggg tcttcatccc
      421 gccgctgcgg gccgcgtccc tgatctcccc ctcgtcggcc ttcctcagga taagccttcg
      481 catgacctcg tcgacaagga gcagctcgaa gatccccatc cttccgcgaa agccggtgta
      541 cgtgcaggcc tcgcagcccc tgcccctgta gaggcgggcg tcgccgcgga tgcccagggc
      601 ctccagttcc tgcgcctcgt cagttgacgt gtcctcggcc ttgcacgtga ggcagatcac
      661 cctgacgagc ctctgggcta ggatgccccg tacggtggag gacaggagaa agctctccac
      721 ccccatgtcg aggagccggg tgatggcgct gggggcatcg tttgtgtgga gcgtcgagaa
      781 gacaaggtgg cccgtgaggg ctgcctgtat ggcgatctcg gccgtctcga ggtcccgtat
      841 ctctccgatc atgaccacgt ccgggtcctg ccgcactatg tgcctcaagg tgttggtgaa
      901 gctgagcccg atgttcggtc tcacctgtat ctggttgaca cccttcaact ggtactcgat
      961 cgggtcctcg acggttatga tcttcttgtc gggcgagttt atcttgtcaa gggcgccata
     1021 cagcgtcgtg gtcttgccgc tgcccgtcgg ccccgtgacg agtattattc cgttgggcct
     1081 ctttatgagc cggttgaaat tattcagtgt gtcgggcatg aaacccaggg catcgaggtc
     1141 gatgacgatg ctctccttgt cgaggatcct catgacgatg ctctctccgt gaagaatggg
     1201 tatcgtggat accctgaggt cgatctcgcg gtcccccacg agaagcctga tgcggccgtc
     1261 ctggggaagc ctgcgctcgg cgatgttcag tttcgccatg atcttgatcc tcgataccat
     1321 ggccgcctgg agccgtttcg gcgccgattc cacatcgctc aggaccccgt ctatgcggta
     1381 gcgcaccttc acctcgtccc cgaaaggctc gatgtggatg tcgctcgccc tgctttccac
     1441 cgccctcgtg atgaggagat tgacaagctt gatgatgggc gcctcggacg cgaggtcctt
     1501 gagatggccg atgtcctctt cctccggctc gttgaagagc tcctggctct cttcgtccat
     1561 gccttccatg atccggttga tgctctgctc ctcctggctg tagaacttgg acaggtgctc
     1621 gtcgatgacc tccctggcgg cggtgtaaac gatcacctct ccggcagcgg caacgcgcag
     1681 ggcctcgata acctcacgat cgtcaggatc cgccatgatc accttcagga cgtcgttctt
     1741 gaactcaaag gggatgatcc tgttctcgcg aatgaaacgt gtcgatatcc cgtcaattac
     1801 gaggggtacc ttggggaaat cttctgcatc gaggaagatc gcactctctt ttgacactgg
     1861 tgttcctcat ggtccttttt ctattccact ctttccggct gcgtttggaa gccgtcctga
     1921 gccgcgccgc ccttcctttc cgcgccgtcc cgggggaaac cgtcctgtgg agcggccgga
     1981 ggcggaggca ccggcttgat cttgatgagc tcttcccttt tgacctttcc cttccccgtc
     2041 ctggtaacgc tgtccacgta atcggaggtg accgcgttgg cgtcggcgtg ggtgcggatc
     2101 acgcggggcg tgagcaggat gatgagttcc gtcctctggt cgctctgtgt ggtctggccg
     2161 aagagatacc cgaggatggg gatcttgctg agcagaggga taccggaccg ggacttcgag
     2221 atgtcgtccc gtatcagtcc gccgatgatg atggtgttgc cgtcctgaac gacgaggctc
     2281 gtcgtcgtct ccgtcttctt caggatgatg tagtcctcgt tgtcgaagag cttgatcgtc
     2341 tcgtaggacg agacctcctg ggtgatgtcg agggtcacga ggccgccctc gttgatgcgg
     2401 ggcttcacct tgaggatgat cccgatgtcc ttgtactgga tcgtccgctg gggcgccacc
     2461 gttcccgaac cgaagg
//
LOCUS       DAOPOA010000339         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46099_length_2473_cov_7.288668, whole genome shotgun sequence.
ACCESSION   DAOPOA010000339 DAOPOA010000000
VERSION     DAOPOA010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46099_length_2473_cov_7.288668"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..210)
                     /gene="rpmG"
                     /locus_tag="PKJ17_07530"
     CDS             complement(61..210)
                     /gene="rpmG"
                     /locus_tag="PKJ17_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOD75860.1"
                     /translation="MRQLVILACGECKRRNYTTTKNKQKTPDRLEMKKYCSSCRKHTS
                     HRETK"
     gene            complement(223..1422)
                     /gene="tuf"
                     /locus_tag="PKJ17_07535"
     CDS             complement(223..1422)
                     /gene="tuf"
                     /locus_tag="PKJ17_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOD75861.1"
                     /translation="MGKKKFERTKPHVNIGTIGHIDHGKTTLTSAITRCLSNKGQADW
                     IPFDQIDKAPEEKERGITIATAHVEYETDNRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVGANDGPMPQTREHILLARQVGVPCMVVYLNKTDMVDDPELIELVEMELRELLTQ
                     YEFPGDDIPIIKGSALKALEHGCGDPNCPDCKSIYELLAACDSYIPEPARDTDKPFLM
                     PVEDVFTISGRGTVVTGRIERGAIKPGEEVEIVGFRPTQKTVTTSVEMFRKILDQGMA
                     GDNVGLLLRGTKKDEVERGQVIAKPGSITPHTKFKGEVYILTKEEGGRHTPFFSGYRP
                     QFYLRTTDVTGVAKLPDGVEMVMPGDNVTLSVELVTPIALEKELRFAIREGGKTVGAG
                     VVTEIIE"
     gene            complement(1415..>2473)
                     /locus_tag="PKJ17_07540"
     CDS             complement(1415..>2473)
                     /locus_tag="PKJ17_07540"
                     /inference="COORDINATES: protein motif:HMM:NF015706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOD75862.1"
                     /translation="DRSERVGRILRLHANKREEVKKVSAGDICAIVGLKNAVTGDTLA
                     STGHDVVFETIEIPSPVLSCAIAPKKKDDLKKMWLVFNKYTVEDPSLTVRLDGETGEV
                     ILSGMGELHLEIIMDRAKREHNLEMLTSPPQVAYRETITKPASAVGKYIKQSGGRGQY
                     GHVVINIAPHDGTDFIFENKIIGGAIPREFIGSVETGIREALEKGVYLGYPVINVKVE
                     LTDGSFHDVDSSELAFKLAGSIAVKDAVSKANPVILEPIMKVDIHCPPESMGAVIGDA
                     TSRRGRVLEVDERNGFKHVLAHIPLDEMFGYTTQLRSLTQGRGYSSMEFFHYHPVPSN
                     ILQNLKRKDKFATEVLYG"
BASE COUNT          394 a          724 c          759 g          596 t
ORIGIN      
        1 ccccaacacc cggatttgga gtccggtgct ctatccgtta gagctactgg cctttgtgcg
       61 ctatttcgtc tccctatggc ttgtgtgctt cctgcaggag ctgcaatatt ttttcatttc
      121 aagcctgtca ggcgtcttct gcttgttctt cgtcgtcgta tagttgcgcc ttttacattc
      181 cccacaggcc agtataacta gttgccgcat tgtaattccc ctttattcga ttatctcggt
      241 gacgactccg gcaccgactg tcttgccgcc ctcgcggatg gcgaaacgga gctccttttc
      301 cagcgcgatg ggggtgacga gctcgacgga gagcgtcacg ttgtcgccgg gcatgaccat
      361 ctcgacgccg tcgggcagct tcgccacgcc cgtcacgtcc gtggtcctca ggtagaactg
      421 gggacggtag ccggagaaga agggtgtgtg gcgcccgccc tcttccttcg tcaggatgta
      481 gacctcgccc ttgaacttcg tgtggggcgt gatggagccg ggcttcgcga tgacctggcc
      541 gcgctccacc tcgtccttct tcgtgccgcg gagcaggagg ccgacgttgt cgcccgccat
      601 gccctggtcg aggatcttgc ggaacatctc cacggaggtg gtgacggtct tctgggtggg
      661 acggaagccg acgatctcga cctcttcgcc gggcttgatg gcgcctcttt cgatcctgcc
      721 cgtcacgacg gtgccgcggc cggagatggt gaagacgtcc tcgacgggca tcaggaaggg
      781 cttgtcggtg tcacgggccg gctcggggat ataggagtcg caggcagcca gaagctcgta
      841 gatgctcttg cagtccgggc agttcgggtc gccgcagccg tgctcgaggg ccttcagcgc
      901 ggatcccttg atgatgggga tgtcgtcgcc ggggaactcg tactgggtga ggagctcgcg
      961 gagctccatc tcgacgagct cgatgagctc ggggtcgtcg accatgtccg tcttgttgag
     1021 gtagacgacc atgcagggga cgccgacctg gcgggcaagg agtatgtgct cacgggtctg
     1081 gggcatgggg ccgtcgttgg ctcccacgac caggatggcg ccgtccatct gggcggctcc
     1141 cgtgatcatg ttcttgatgt agtcggcatg gcccgggcag tccacgtggg cgtagtggcg
     1201 gttgtccgtc tcgtactcga cgtgggcggt ggcgatggtg atgcccctct ccttctcctc
     1261 gggtgccttg tcgatctggt cgaaggggat ccagtcggcc tgacctttat tggaaagaca
     1321 cctcgtgatg gcgctcgtga gggtggtctt cccgtgatct atatgtccga tggttcctat
     1381 gttcacatgt ggtttcgtcc gttcaaactt tttcttaccc atagaggacc tccgtggcga
     1441 atttatcttt tctttttaga ttctgtaata tgttggatgg taccggatga tagtggaaaa
     1501 actccatgct ggaataaccg cgtccctgcg tcagtgacct cagctgcgtc gtgtagccga
     1561 acatctcatc gaggggaatg tgggccagga cgtgcttgaa gccgttgcgt tcgtccactt
     1621 ccaggaccct gcccctgcgc gacgtcgcgt cccctatcac ggcacccata gactcgggcg
     1681 gacagtggat gtcgaccttc atgatgggct cgaggatgac cgggttcgcc ttcgacacgg
     1741 catccttcac cgcgatggat ccggcgagct tgaacgccaa ctcggaagag tcgacatcgt
     1801 ggaaggagcc atccgtcaat tccaccttga cgttgatgac ggggtagccg agatagacac
     1861 ccttttcgag tgcttccctg atccctgtct cgacgctgcc tatgaattcc ctcggtatgg
     1921 ccccgccgat gatcttgttc tcgaagatga agtccgtccc gtcgtgaggc gcgatgttga
     1981 tgacgacgtg cccgtactgg ccgcggccgc ccgactgctt gatgtacttt cctaccgctg
     2041 aggccggctt cgtgatggtc tcgcgatagg cgacctgggg cggtgaggtc agcatctcga
     2101 gattatgctc cctcttggcc ctgtccatta tgatctcgag gtggagctcg cccatccccg
     2161 agaggatgac ctcgcccgtt tcgccgtcga gcctgaccgt cagcgagggg tcttcaacgg
     2221 tgtacttgtt gaagacaagc cacatcttct tcaggtcgtc cttctttttc ggagcgatcg
     2281 cacaggacag gacgggtgag gggatctcga tggtctcgaa gacgacatcg tgcccggtgg
     2341 acgccagagt atcgcccgta acggcgttct tgagtcccac gatagcgcag atatcgccgg
     2401 cgctgacctt cttcacctct tcgcgcttgt tcgcgtggag cctcagtatc ctccccacgc
     2461 gctccgatct gtc
//
LOCUS       DAOPOA010000340         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46108_length_2473_cov_3.465674, whole genome shotgun sequence.
ACCESSION   DAOPOA010000340 DAOPOA010000000
VERSION     DAOPOA010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46108_length_2473_cov_3.465674"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PKJ17_07545"
     CDS             complement(<1..321)
                     /locus_tag="PKJ17_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOD75863.1"
                     /translation="MLEVRIKELKKELVEYATLVEKMIAESMTGLLERREAPLRRVID
                     ELEPATNDFEIELDELCTGAIAQYQPRARDLRIILMIFKMTSDLERMGDHVANIADSG
                     LFLIS"
     gene            complement(335..1093)
                     /gene="pstB"
                     /locus_tag="PKJ17_07550"
     CDS             complement(335..1093)
                     /gene="pstB"
                     /locus_tag="PKJ17_07550"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOD75864.1"
                     /translation="MEDTVLKVEKFSAFFGDNRVLKDVDLTVRRNVVAALMGPSGCGK
                     TTLIRCVNRMHELIPGAGVAGKMTLVDEDIYTMQPIILRRKVGMVFQKPNPFPTMSIY
                     ENVIAGYKLNGIRMAKSELDTIVERSLKRSALWDEVKDALHRKGTFLSGGQQQRLCIA
                     RALAMNPEILLLDEPTSALDPKSTSQIEELFVELKRNVTMLLVTHNIAQAARVSDVTA
                     FIYLGELVEFGPTEKMFTVPKDKRTEEYLTGKFG"
     gene            complement(1075..1923)
                     /gene="pstA"
                     /locus_tag="PKJ17_07555"
     CDS             complement(1075..1923)
                     /gene="pstA"
                     /locus_tag="PKJ17_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOD75865.1"
                     /translation="MTGGHVGRRLAKDTLFKGAVCLLAFLSLLPLFLILYYITMKGIS
                     VINWDFLFSLPRPIGESGGGVANAVVGTLMLIALSSVISVPCGIAAGIYLSENKTGKL
                     SNTIRLSVIVLQGTPSIVIGIIAYLWVVAPLKTFSALSGGVALSIMMLPVIIKTTEET
                     LKLIPYHLKEASLALGVPYYRTILKVVLPSGISGILTGILLSVARITGETAPLLFTAF
                     GNQFMNVNIFKPIDSLPYRIFYYAMSPYPEWHSFAWGASFILVVLTLALNLIARGISA
                     KWKIQF"
     gene            complement(1920..>2473)
                     /locus_tag="PKJ17_07560"
     CDS             complement(1920..>2473)
                     /locus_tag="PKJ17_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOD75866.1"
                     /translation="IPSVIYGLWGLFFLMPAVRFIEMKLGVAPHGVGILTSSLILAIM
                     IIPFSASIGREVITLVPGDLKEAAYSLGATRFEVIRNIVIPYARSGIIAGILLALGRA
                     IGETMAVTMLIGNSNFLPTSIFSPANTMASVIANEFAEATGVTAASLIYVALVLFLVT
                     MVVNIIGTYVIQRISIEASREVS"
BASE COUNT          508 a          704 c          740 g          521 t
ORIGIN      
        1 ggatatgagg aacaatccgc tgtcggcaat gttcgcgacg tgatctccca tccgttccag
       61 atcgctcgtc atcttgaaga tcatgaggat gatgcgcaga tcccgcgccc tcggctggta
      121 ctgcgctatg gcccccgtgc acagctcgtc gagttcgatc tcgaaatcgt tcgtcgccgg
      181 ctccagttcg tcaatgaccc ggcgaagagg ggcctcccgc ctctccagaa gacccgtcat
      241 gctctcggcg atcatcttct ccaccagggt ggcgtattcg acgagctctt tcttgagctc
      301 ctttatcctg acctccagca tcccttttag cctcctatcc gaattttccc gtcaggtatt
      361 cttccgtccg cttgtccttc ggcacggtga acatcttctc cgtcggcccg aactcgacga
      421 gttccccgag gtagatgaag gccgtgacat cggagaccct cgcggcctgg gctatgttgt
      481 gggtgacgag aagcatggtg acgttcctct tgagttccac gaagagctcc tcgatctgcg
      541 acgtcgattt cggatcgagg gccgacgtcg gttcgtcaag gagcagtatc tcggggttca
      601 tggcaagcgc ccgggctatg cagagccttt gctgctgtcc cccggagaga aaggtcccct
      661 tccggtgaag ggcatccttg acctcgtccc agagggccga tctcttcagc gaccgttcga
      721 cgatagtgtc cagttccgat ttcgccatcc tgatgccgtt gagcttgtat cccgcgatga
      781 cattctcgta tatgctcatc gtgggaaagg ggttcggttt ttgaaagacc attcccactt
      841 tccgtctcag gataatgggc tgcatcgtgt agatgtcctc gtcgacaagc gtcatcttcc
      901 ccgccacgcc ggcgccgggg atgagttcgt gcatcctgtt gacgcagcgt atgagggtcg
      961 tcttaccgca ccccgacggt cccatgaggg cggcgacgac gttcctcctg acggtgaggt
     1021 caacgtcctt gagcaccctg ttgtcgccga agaaggcgct gaacttctcg acctttagaa
     1081 ctgtatcttc catttagcgg agatccccct tgcaataagg ttcagggcaa gcgtcagaac
     1141 gacaagtatg aacgaggccc cccacgcgaa ggaatgccac tcggggtagg ggctcatggc
     1201 gtaatagaat atgcggtagg gcagggaatc tatcggcttg aagatattga cgttcatgaa
     1261 ctgattgccg aatgccgtga agagaagcgg tgccgtttcc cctgtgatcc tcgccacgct
     1321 gaggaggatg cccgtgagga tgccgcttat gcccgagggg agtacgacct tgagaatggt
     1381 ccggtaatag ggcacgccga gggccagcga ggcctccttc aggtgatagg gtatgagctt
     1441 cagggtctcc tccgtggtct tgatgatcac gggaagcatc atgatggaaa gggcgacacc
     1501 gccggagaga gcggaaaagg tcttcagggg agccactacc cagagatagg cgatgatgcc
     1561 gatcactatc gagggcgttc cctggaggac aatgacacta aggcgtatgg tattgctcag
     1621 ttttccggtc ttgttctcgg aaaggtagat gccggccgct atcccgcagg gcacggatat
     1681 caccgaggac agggcgatga gcatcagcgt ccccacgacg gcgttggcga cgcctccccc
     1741 ggactcgccg atggggcgcg gcaggctgaa gagaaaatcc cagttgatca ccgatattcc
     1801 cttcatcgtt atgtaataga ggatgagaaa gagggggaga agggacagga aggcaagaag
     1861 gcagaccgct cccttgaaga gcgtgtcctt cgcaaggcgc ctgccgacat gaccgcccgt
     1921 catgagacct ccctggatgc ttcaatgctg atcctctgaa tgacgtatgt cccgatgatg
     1981 ttgacgacca tggtcaccag aaagaggacg agggcgacat agatgagcga cgccgcggtg
     2041 acgcccgtcg cctctgcgaa ctcgttggcg atgacgcttg ccatcgtgtt ggcgggactg
     2101 aatatgctcg tcggcaggaa gttgctgttg ccgatgagca tcgtcaccgc catggtctcg
     2161 cctatcgccc gtcccagcgc gaggagtatg cccgcgatga tgccggaccg cgcgtagggg
     2221 ataacgatgt tccgtatcac ctcgaaccgc gtcgctccca gcgagtaagc cgcctccttc
     2281 aggtcgcccg gcaccagcgt gatcacctcc ctgccgatgg acgccgaaaa ggggatgatc
     2341 atgatggcaa ggatgagcga cgaggtaagt atccccacgc catggggggc gacgcccagt
     2401 ttcatctcta tgaaccgcac agcgggcatg agaaagaaga ggccccagag accgtagatg
     2461 acggaaggga tcc
//
LOCUS       DAOPOA010000341         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46112_length_2473_cov_3.089330, whole genome shotgun sequence.
ACCESSION   DAOPOA010000341 DAOPOA010000000
VERSION     DAOPOA010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46112_length_2473_cov_3.089330"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PKJ17_07565"
     CDS             complement(<1..1140)
                     /locus_tag="PKJ17_07565"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOD75867.1"
                     /translation="MDKKIFLNEKDMPRAWYNIQADLETPLPPPLNPETGEPVTPDML
                     EAIFPMNLIEQEVSTERFIDIPGEVMERLLMWRPTPLCRATGLEKFLGTPAHIYYKNE
                     GVSPPGSHKPNTAIAQAYYNKEAGIKRLTTETGAGQWGSALAFACNQFGIELKIYMVR
                     VSFNQKPYRRVMMETWGGKCVPSPSPDTKAGRAFLEGNPDHPGSLGMAISEAIEDAVT
                     SKNTKYSLGSVLNHVLLHQSVIGLEAQRQLELAGEYPDVVIGCAGGGSNFAGIAFPFI
                     RDKIHGKEIRVIAAEPTSCPTLTRGRYVYDFGDTAGMTPLLPMYSIGHGFVPAPIHAG
                     GLRYHGMAPLVSKVFADGLIEARAYNQIETFSAGVIFARTEGFIPA"
     gene            1599..2243
                     /gene="fsa"
                     /locus_tag="PKJ17_07570"
     CDS             1599..2243
                     /gene="fsa"
                     /locus_tag="PKJ17_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOD75868.1"
                     /translation="MKFFIDTANINEIRKGMEMGLVDGVTTNPSLLAKEKKDPDAVLR
                     EILQTVPGPVSLEVISTTAEGMCEEARKLAALGENAVVKIPMTGEGIRAVRTLTDEGI
                     KTNVTLIFQPVQALIAAKAGATYVSPFIGRLDDISQRGMDIIENIVTIFYNYAFETEV
                     IVASIRNPTHVLDAALVGADIATIPFNVLSQLMNHPLTDIGLQKFLKDWEGIKK"
     gene            complement(2233..>2473)
                     /locus_tag="PKJ17_07575"
     CDS             complement(2233..>2473)
                     /locus_tag="PKJ17_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75869.1"
                     /translation="ADLIILDVVMPKMNGKEAYDRLRSIDPNIRVLFMSGYTDDIIHQ
                     KGILDETIEYIAKPILPRDLLKKVREMLDGETPIS"
BASE COUNT          512 a          709 c          687 g          565 t
ORIGIN      
        1 cgccgggatg aacccttccg tccgggcgaa gataacgccc gccgagaacg tctcgatctg
       61 gttgtatgcg cgcgcctcga tgagcccgtc ggcgaagacc ttgctgacga ggggtgccat
      121 gccgtgatac cgaaggcccc ccgcgtgtat cggcgcggga acgaatccgt gcccgatgga
      181 gtacatgggc agaagcggcg tcatcccggc ggtgtccccg aaatcataga cataccggcc
      241 cctggtcaac gtcggacagg aggtgggctc cgcggctatc acccgtatct ctttcccgtg
      301 gatcttgtca cggatgaaag ggaaggctat gccggcgaag ttgctgccac cgcccgcgca
      361 cccgatgacg acatcggggt attctcccgc gagctcgagc tgccgctgtg cttcaaggcc
      421 gataacggac tggtggagga ggacgtggtt gaggacgctg cccagggagt acttcgtgtt
      481 cttcgaggtc accgcgtcct cgatggcctc actgatcgcc attccgaggc ttcccgggtg
      541 gtccgggttc ccttcgagga acgcgcgccc ggctttcgtg tcgggactcg ggctgggcac
      601 gcatttccct ccccaggtct ccatcatgac gcgccggtac ggcttctggt tgaagctcac
      661 acggaccatg tagatcttca gctcgatgcc gaactggttg caggcgaagg caagggcgct
      721 cccccactgt ccggcccccg tctccgtcgt gagcctcttg atgccggcct ccttgttata
      781 gtaggcctgc gcgatggccg tgttcggctt gtggcttccc ggggggctga ccccttcgtt
      841 cttataatag atgtgggcgg gcgtcccgag gaacttttca agccccgtgg cccggcacaa
      901 tggcgtcggc ctccacatga ggagtctttc catgacctca ccgggaatgt cgatgaacct
      961 ctcggtgctc acctcctgct cgatgaggtt catcgggaag atggcctcga gcatgtccgg
     1021 cgtcacgggc tcccccgttt ccgggttgag cggcgggggc aggggcgtct caagatcggc
     1081 ctgaatattg taccaggccc gcggcatgtc cttttcattg aggaatattt tcttatccat
     1141 gataccctcc tttctttcgg taacagaaag ggccatgagc ttgtctgccc atggcccttt
     1201 tcaaataaaa cggccatgga cggtgtgaaa ccgtccatgg ccggaaaatt acggtctcac
     1261 acctacaatc tccgccacca ccaccagttg tttgtcgctg cgttcgtcat aatgaggctt
     1321 tataacatgg gaaatggagg atgtcaaaat aaaagataat gaccaatgac caaattccaa
     1381 tgaccagata aacagacaat aaccaaaaag agaacgatac aggcagtcca ttctgtcctt
     1441 ctctttgttc aattggttat tggtacattg gtcattatcc tcttcattgg tcattggaat
     1501 ttggtcaatt ggtcattctc ttcttattgg tcattctctt cataatcgtg tatactctta
     1561 cagtttttat atccaccatc atgttcggag gtcaggaaat gaaattcttt atagatacgg
     1621 caaatataaa cgagatcaga aagggaatgg agatggggct cgtcgatggc gtgaccacca
     1681 atccgtcact tctcgcaaaa gagaagaagg accccgacgc cgtgctccgc gagatacttc
     1741 agaccgttcc cgggcccgtc agcctggaag tcatatcgac cacggcggaa ggcatgtgcg
     1801 aggaggctcg gaaactcgcc gcgctgggcg agaacgccgt cgtcaagata cccatgacgg
     1861 gtgagggtat ccgggccgtc aggacgttga ccgacgaggg catcaagacg aatgtgaccc
     1921 tcatctttca gcccgttcag gccctcattg ccgcgaaggc gggcgccacg tacgtgagcc
     1981 cctttatcgg caggctcgat gatatatctc agaggggcat ggatatcatc gagaacatcg
     2041 ttaccatatt ctacaactac gcctttgaga cggaggtcat cgtcgcaagc atccgtaacc
     2101 ccacgcacgt cctcgacgcg gcccttgtcg gcgccgatat tgccaccatc cccttcaacg
     2161 tcctctcgca gctcatgaat catcccctga cggacatcgg cctgcagaaa ttcctcaagg
     2221 actgggaggg catcaagaaa taggggtttc tccgtcgagc atctccctga cctttttcag
     2281 aaggtcccgg ggcagaatgg gcttcgcgat gtactctatc gtctcgtcaa ggatgccctt
     2341 ctggtggatg atgtcatcgg tgtagccgct catgaaaagc accctgatat tgggatcgat
     2401 ggatctcagc ctgtcatagg cctctttgcc gttcatcttc ggcatgacga catcgaggat
     2461 gatgaggtcc gcc
//
LOCUS       DAOPOA010000342         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46284_length_2467_cov_2.665837, whole genome shotgun sequence.
ACCESSION   DAOPOA010000342 DAOPOA010000000
VERSION     DAOPOA010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46284_length_2467_cov_2.665837"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PKJ17_07580"
     CDS             <1..991
                     /locus_tag="PKJ17_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75870.1"
                     /translation="KAFCDLHDYAVAEVVGRPYLDFIAPESRSEVRKIYETRVERGAA
                     QDLYMYFRRTRDGRSLPTENKVKLIIYRGEKAAVGICRDITERVEMEKKVRESESLAH
                     IGQLTTSLAHEIRNPLSSVKMNIQILLKKIEFKGNDKRRMEIMTEEISRLERILTEML
                     DFAKPLRLDLKPASIRSIILSCLEMMTVRFQEKGIEVRTSFSRETPRIFIDRDKIEQA
                     VINVLQNSIDVLPEGGKIGIRTKYAAGNGRFVSIDVDDNGPGIADGDLPYVFDPFFSN
                     KKKGTGLGLWNVKKVIEAHGGTVGITTKRTGGVHLSMTVPVRRRNNEKKDTHR"
     gene            966..2357
                     /locus_tag="PKJ17_07585"
     CDS             966..2357
                     /locus_tag="PKJ17_07585"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD75871.1"
                     /translation="MKKRILIVDDEKSLLESLEMFLSEKGYNVACAMTAAEAIEKNGS
                     FKPDVIVLDIRLPDKDGLEVLKEFRETTRKSNVIIITAFHDMDTTIKAMKFGACEYIP
                     KPIDIEELESAVERAARAGAPGPAANAISLDPSLIYEKGKIIGKSREMKEIFKAIGIL
                     SENKVTVLVEGETGTGKEMIARAIHYHSPSKDAPFIGINCSSIVGTLLESELFGHEKG
                     SFTGAIATKKGKFELAGEGTIFLDEVSEIPFELQAKLLRFLQEKEFEHVGGERSLKSN
                     ARVIAATNRDLWQMVREGHFREDLFYRLSVAMIRVPPLRDRRSDIPLLIQYLLKKINA
                     DLHHSIKRVEGRAMERLLEYHWPGNVRELENVLTRGVISTPGEVILDEFIVTLLEKTD
                     AVEEDGRDAPTAMTTLQDVEKVHILKVLQHTGWHFGKTCGILGISRPTLRQKLKEYDI
                     TLPPDHSRSSLAP"
BASE COUNT          615 a          683 c          741 g          428 t
ORIGIN      
        1 caaggccttt tgcgatctcc acgattacgc cgtggccgag gtcgtcggca ggccctacct
       61 tgatttcatc gcgcccgagt cccgcagcga ggtgaggaag atctacgaga cccgggtgga
      121 gaggggggcc gcccaggacc tctacatgta tttccgccgc acccgggatg gacgttctct
      181 ccccaccgag aacaaggtca agctcatcat ctaccggggg gagaaggcgg ccgtcggcat
      241 ttgcagggac ataacggaac gggtcgaaat ggagaagaag gtccgggaat cggagagtct
      301 tgcccacatc ggacagctca cgacctctct cgcccacgag atccgcaacc ccctgtcttc
      361 cgtcaagatg aacatccaga tactcctcaa gaagatcgag ttcaagggga acgacaagcg
      421 gcggatggag atcatgacgg aggagatatc ccgcctcgag aggatcctca cggagatgct
      481 ggatttcgcg aaacccctca ggctggacct taagccggca tcgatcaggt ccatcatcct
      541 ctcctgcctc gagatgatga cggtaaggtt ccaggaaaag ggcatagaag tgcgcacatc
      601 gttctcgagg gagaccccga ggatattcat agaccgcgac aagatcgagc aggccgtcat
      661 caatgtcctg cagaactcca tcgacgtgct tcccgagggg ggaaagatcg ggataaggac
      721 gaagtatgcc gccgggaacg gcaggttcgt cagcatagac gtcgatgaca acggaccggg
      781 catagcggac ggagaccttc cttacgtttt cgatcctttc ttcagcaaca agaagaaggg
      841 caccggactg gggctgtgga atgtgaagaa ggtcattgag gcacacgggg ggacggtggg
      901 tattaccacg aaacgaacgg ggggcgttca cctctccatg acggtacccg tcaggaggcg
      961 caataatgaa aaaaaggata ctcatcgttg acgatgagaa gtcgcttctg gaatcccttg
     1021 agatgttcct gagcgagaag ggctacaatg tcgcctgtgc catgacggcc gccgaggcga
     1081 tcgagaagaa tggctccttc aagccggacg tgatcgttct cgatatccgt ctccccgaca
     1141 aggacggcct cgaggtgctg aaggaattcc gggagaccac gagaaagagc aacgtcatca
     1201 tcataaccgc ctttcacgac atggacacaa cgatcaaggc gatgaagttc ggcgcctgcg
     1261 agtacattcc gaaacccatc gacatcgagg aactggagag cgccgtcgag cgcgcggcgc
     1321 gtgccggagc ccccgggccg gccgcgaacg ccatatctct cgatccttcc ctcatttacg
     1381 aaaaagggaa gatcatcgga aaaagccggg agatgaaaga gatattcaag gcgatcggga
     1441 tactgtcgga gaacaaggtg acggtcctgg tagagggaga aacgggaacg ggcaaggaga
     1501 tgatagcgcg ggccatccac tatcacagcc cgtcgaagga cgcccctttc atcgggatca
     1561 actgttcctc catcgtgggg acacttcttg aaagcgaact cttcgggcac gagaagggtt
     1621 ctttcacggg cgccatcgcg acaaagaaag gaaagttcga gcttgcagga gagggcacca
     1681 tcttcctcga cgaggtgagc gagataccct tcgagctgca ggccaaactg ctccgcttcc
     1741 tgcaggagaa ggagttcgag cacgtcgggg gcgagaggag cctcaagtcc aacgcgcggg
     1801 tcatagcggc cacgaacagg gacctctggc agatggtgcg ggaagggcat ttcagggagg
     1861 acctcttcta caggctgagc gtggccatga tcagggtgcc cccgcttcgg gaccggcgct
     1921 ccgacatacc gctgctcata caatacctcc tcaaaaagat caatgccgat ctgcaccaca
     1981 gcatcaagag ggtggaggga agggccatgg agcggcttct cgaatatcac tggcccggca
     2041 acgtccgtga gctggagaac gtcctcacga ggggcgtgat ctccactccc ggcgaggtga
     2101 tcctcgatga gttcatcgtg accctccttg aaaagaccga tgccgtcgaa gaggacggga
     2161 gggacgcgcc gactgccatg acgaccctgc aggacgtgga gaaggtccac atcctcaagg
     2221 tcctgcagca caccgggtgg catttcggca agacctgcgg catcctcggc atatcgcggc
     2281 ccacgctcag gcagaaattg aaggagtacg acataaccct tcctcccgat cactcccgat
     2341 catcgctggc accctaattg caattagcat ttccgtgacc ccttcaatga agacgctgca
     2401 ggagatacga aaggagcaca tctgcaaagt gctcgaaaag acacattggg atttgaaaga
     2461 ggccagt
//
LOCUS       DAOPOA010000343         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46298_length_2466_cov_3.587723, whole genome shotgun sequence.
ACCESSION   DAOPOA010000343 DAOPOA010000000
VERSION     DAOPOA010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46298_length_2466_cov_3.587723"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2445)
                     /locus_tag="PKJ17_07590"
     CDS             complement(<1..2445)
                     /locus_tag="PKJ17_07590"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75872.1"
                     /translation="MMSTNNILSPANGKPIIVPTQDIVLGLYYLTIDRKYRRGENKIF
                     ADSEEVRVAYDSGEIDLHARVRVRLNGEMVNTTPGRIILRDVVQDPLMELPQPKRDTV
                     LRDMFSNINTIMDKKTIAALVDKCYRDAGEKCTVILSDRLKDLGYSYATLGGISISID
                     DMKIPQKKRVLIEKAEEAVKTVQNQYQEGLITDGERYNQVIDIWANVTEEIAKALMDE
                     LGSDVVLDMSGKPVIGANGKPEHQNSLNPIFMMAHSGARGNAQQIRQLAGMRGLMAKP
                     SGEIIETPITSNFREGLDVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDVV
                     VSENDCGTFDYIEIGSLVEGGEVIERLDARILGRVSFEDMKDPDGAIIVHKNEEITEH
                     HLKLIEEAGFEKVKIRSVLTCRARRGVCVLCYGRDLARGRLVSIGEAVGIIAAQSIGE
                     PGTQLTMRTFHIGGAASRRVEQSTLETRNDGIVKFINVRAILNREDIPVVMNRNGEIA
                     IMDDAGRERERYSIIYGAKLRVKDGQTVEEGETLAEWDPYTIPILSEETGKIKYGDIF
                     EGETMQESKDEVTGLSYKVIIEPKNPELRPRISIKDEKGRTKMIPGSTSPARYILPIG
                     AHIVVNEGDEIFAGDVISKMPRETTKTKDITGGLPRVAELFEARKPKENAIVTEINGV
                     VTFGKMAKGKREIVVTPDTVHGEPRKYTIPRGKHVIVHEGDYVRAGEPLMDGPVNPHD
                     VLRILGIKDLARYLVDEIQEVYQLQGVKINDKHIETIVRQMLKRVRIKDIGDTNFIID
                     DYVEWWVFEEENRRVLS"
BASE COUNT          400 a          756 c          694 g          616 t
ORIGIN      
        1 tgacaggacg cgcctgttct cctcttcgaa gacccaccat tccacgtaat cgtctatgat
       61 aaaattggtg tcccctatgt ccttgatacg caccctcttg agcatctgcc gcacgatcgt
      121 ctcgatgtgc ttgtcgttta tcttgacgcc ctggagctgg tagacttcct ggatctcgtc
      181 cacgagatac cgcgccaggt ccttgatgcc gagtatcctc agaacgtcat gggggttcac
      241 ggggccgtcc atgagcggtt caccggccct gacataatcg ccctcgtgga cgatgacgtg
      301 cttgccccgt ggaatggtgt atttccttgg ctcaccgtgc acggtgtcgg gagtcacgac
      361 gatctccctc tttcccttgg ccatcttgcc gaaggtgaca acgccgttga tctctgtgac
      421 gatggcgttc tccttcggct tgcgtgcttc gaaaagctcg gcgacacggg gaagaccgcc
      481 ggtgatgtcc ttcgtcttcg tggtctcacg gggcatcttg gaaatgacgt cgcccgcgaa
      541 gatctcgtca ccctcattga caacgatgtg tgcccctatg gggagaatgt accgggcggg
      601 actcgtcgac ccggggatca tcttcgtcct gcccttctca tccttgatgg agatcctcgg
      661 gcggagctcc gggttcttcg gctcgatgat gaccttgtat gaaagacccg tcacctcgtc
      721 cttgctctcc tgcatggtct cgccttcgaa tatgtcgcca tacttgatct taccggtctc
      781 ttccgagagt atggggatgg tgtagggatc ccattcggcc agcgtctctc cctcttcgac
      841 cgtctgtccg tctttgaccc tgagctttgc cccgtagatg atggagtagc gctctctttc
      901 ccttcccgcg tcgtccatga tggcgatctc gccgttgcgg ttcatcacga cgggaatatc
      961 ctcgcggttg aggatggccc tcacattgat gaacttgacg ataccgtcgt tcctcgtttc
     1021 cagcgtcgac tgctcgaccc ttctggacgc ggcgccgccg atgtggaagg tcctcatcgt
     1081 gagctgcgta ccgggctcgc cgatcgactg cgccgcgatg atgcccacgg cctcgccgat
     1141 gctcaccaac cgtccgcgcg cgagatcgcg gccgtagcac agcacgcaca ctcctcttcg
     1201 cgcccggcag gtcagcacgg agcgtatctt caccttttca aacccggctt cttcgatgag
     1261 cttgaggtgg tgttccgtta tctcttcgtt cttgtggacg atgatcgctc catcggggtc
     1321 tttcatatcc tcgaaggaaa cgcggccgag gatacgcgcg tcgaggcgtt cgatgacctc
     1381 gccgccctcg acaagagacc cgatctcgat gtagtcgaag gtcccgcagt cgttctcgga
     1441 gacaaccacg tcctgggcga cgtcgacaag ccttctcgta aggtaaccgg agttcgcggt
     1501 cttgagggcc gtgtcggcga ggcccttcct ggcgccgtgc gtggatatga agtactggag
     1561 aacgtcgagc ccctcgcgga agttgctcgt gatgggcgtt tcgatgatct cgccggaagg
     1621 tttggccatg agccccctca tgccggcgag ctgtctgatc tgctgcgcgt tgcctctcgc
     1681 gccggagtgg gccatcatga agatcgggtt caggctgttc tggtgttccg gcttgccgtt
     1741 cgccccgatg acaggtttac ccgacatgtc gaggacgaca tcagagccga gttcgtccat
     1801 caacgccttt gcgatctcct ccgtcacgtt cgcccagatg tcgatgacct ggttgtaccg
     1861 ctcgccgtcc gttatgagac cttcctggta ctggttctga accgtcttca cggcctcttc
     1921 ggccttctct atgagcactc tcttcttctg ggggatcttc atgtcgtcga tggagatgga
     1981 gatgcctccc agcgtcgcgt aactgtagcc gaggtctttc aacctgtcgg aaagaataac
     2041 ggtgcatttc tcgcccgcgt cgcggtagca cttatccacc agggccgcga tggtcttctt
     2101 gtccatgatc gtattgatgt tcgagaacat gtccctgagg accgtgtccc tcttcggctg
     2161 gggcagctcc atgagagggt cctgcacgac atccctcaag attatcctcc ccggcgtggt
     2221 gttcaccatc tcgccgttga ggcgcaccct gaccctcgcg tggaggtcga tctctcccga
     2281 gtcgtaggcc actcggacct cttcggaatc ggcgaatatc ttgttctctc ccctgcgata
     2341 tttccggtcg atggtgaggt agtaaagccc gagcactatg tcctgcgtgg gaacaatgat
     2401 gggcttgccg ttggcggggg aaaggatgtt gttcgtcgac atcatgagca cccgggcctc
     2461 cacctg
//
LOCUS       DAOPOA010000344         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46400_length_2462_cov_3.355214, whole genome shotgun sequence.
ACCESSION   DAOPOA010000344 DAOPOA010000000
VERSION     DAOPOA010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46400_length_2462_cov_3.355214"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..1102)
                     /locus_tag="PKJ17_07595"
     CDS             complement(227..1102)
                     /locus_tag="PKJ17_07595"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOD75873.1"
                     /translation="MTKKCLALLCAPFLLAALASCDTSSFGNISQHIGTAMTVVDQTS
                     KAARPISDEEEYYVGRAVAAKILGSYQLTTNAQLTRYVNLIGRTVALNSDKPYTYGGY
                     HFAILETNEINAFAAPGGTIFITSGMIRTTASEDELAAVLAHEAAHISKRDGIAAIKS
                     ARWTEALTVIGTTAARNYTSGEVSQLVSLFEGSIDDVFKTLVVNGYGRSQEYAADESA
                     VQTLAKCGYDPRALLSFLKRLEQRSGSSGGGITATHPATADRIENISGKIPSTAQIHP
                     RAIEARAVRYGAAVR"
     gene            complement(1099..1563)
                     /locus_tag="PKJ17_07600"
     CDS             complement(1099..1563)
                     /locus_tag="PKJ17_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75874.1"
                     /translation="MMKKAIFLSLIMVFTATAALADTVTVITKENAIREYAKFFAPVK
                     ANVRYNDRLEALSMEGDWYKVKCGPSIGYIHRSAVEKRVVAVPASYSRKGSSVSESEA
                     ALAGKGFNPQVEATYKKQHPEMKFGLVDSVERYPVPERDIAQFIVAGGLRQP"
     gene            complement(1560..>2462)
                     /locus_tag="PKJ17_07605"
     CDS             complement(1560..>2462)
                     /locus_tag="PKJ17_07605"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOD75875.1"
                     /translation="YALEGRERQFIKRTFSQYMDKTIVEHVLQNPDIVRPGGKKARVT
                     VLFADIAGFTTISERNTPEDTALMLHRVLNELTEVVIDEKGVVDKYIGDCIMAFWGSP
                     LETPKDEINACRSSLRFIDAIGGINGVFEREGIPPVAIRVGLHTGDAIAGNMGSVRLF
                     DFTVVGDTVNLASRLESVNKVFGTNIIASEHTVAKTYDLFLSRSLGPIEVKGKSEPVV
                     VHEVLCEAGDAPAALRRKVDLYNEAFQTYREQRFTEAVERFDVLLKEFPHDGPSEFYR
                     RRAESLAGNFSLTKEWDIIRMTEK"
BASE COUNT          423 a          728 c          741 g          570 t
ORIGIN      
        1 ccgccccgcc tccgaccttg taggcttcga cgcgttccgt gtagctgact cccatgtgcg
       61 ctgctgtccc cgtcatggtt acactgtatc gcgggcactg cgccttcgcc ccgaaggatg
      121 agagcggtgc cagcaagagg gcaatgatca tgaggtactt catggaacac ctctcttcgc
      181 caatggttat gctcttttgg ttcttgaggt caaagacacc gcagtcttaa cgaacggcgg
      241 cgccgtatct taccgcgcgg gcctcaatgg ccctggggtg gatctgtgcg gtggagggta
      301 tcttgccgga gatgttctct atcctgtccg cggtggcggg atgtgtcgcc gtgatgccgc
      361 cccccgacga tccgctcctt tgctcgagcc gtttgaggaa agacaggagg gcccggggat
      421 cgtacccgca cttcgcgagg gtctgcacgg cggattcgtc cgccgcgtat tcctgtgacc
      481 ggccgtaccc gttgacgacg agggtcttga agacgtcatc gatgctgccc tcgaagaggc
      541 tgaccagctg ggagacctca ccggaggtgt agttgcgcgc cgccgtggtg ccgatgacgg
      601 tgagcgcctc cgtccagcgc gcgctcttga tcgcggctat gccgtcgcgc ttgcttatgt
      661 gcgcggcctc atgggcgagt accgcggcca gctcatcctc gcttgccgtc gtccgtatca
      721 tgccgctcgt gatgaagata gttccgcccg gggcagcgaa ggcgtttatc tcgttcgtct
      781 ccaggatggc gaaatggtac ccaccgtagg tatagggttt gtcggaattg agggctaccg
      841 tccttccgat gagatttacg tagcgcgtga gctgcgcgtt cgttgtcaat tgataggacc
      901 cgagtatttt tgcggccacg gcgcggccga catagtattc ctcttcgtcg gagatgggac
      961 gggcggcctt ggacgtctgg tccaccacgg tcatcgccgt gccgatgtgc tgcgagatat
     1021 tgccgaagct gctggtgtcg catgaggcga gcgctgccag gagaaagggg gcgcacagga
     1081 gcgcgaggca tttctttgtc atggctgcct caaccccccg gctacgatga actgcgcgat
     1141 gtcccgttcg ggaacaggat agcgctcgac gctgtcaacg agaccgaatt tcatttccgg
     1201 atgctgtttc ttgtatgttg cctcgacctg cgggttgaat cccttcccgg cgagcgccgc
     1261 ctcgctctcg ctgacggagg acccctttcg cgaataggag gcaggaacgg cgacgaccct
     1321 cttctccacg gcgctcctgt ggatgtagcc gatggagggg ccgcacttca ccttgtacca
     1381 gtcgccttcc atggagaggg cctccaacct gtcgttatat cgcacgttgg ccttgacggg
     1441 cgcgaagaac tttgcatact cacggatcgc gttctctttc gtgatgaccg tgaccgtgtc
     1501 ggccagcgcg gcggtcgccg taaagaccat tatcaatgat aggaatatgg ctttcttcat
     1561 catttttcgg tcattctaat aatatcccat tcttttgtca aagaaaaatt ccccgccagg
     1621 gattcggccc gtctcctgta gaattcgctc ggtccgtcgt gggggaattc cttgagaaga
     1681 acgtcaaaac gctccacggc ctcggtgaat cgctgctccc tgtaggtctg gaatgcctcg
     1741 ttataaaggt cgacctttct tctgagtgcg gcgggtgcgt cacctgcctc gcagaggacc
     1801 tcgtggacca cgacgggctc cgatttcccc ttcacctcga tgggacccag gctccgcgac
     1861 aggaagagat cgtaagtctt cgcgacggtg tgttcgctgg caatgatgtt cgtgccgaag
     1921 accttgttga ccgattcgag gcgtgatgcc aggttcaccg tgtcgccgac gacggtgaag
     1981 tcgaaaagcc gcacgcttcc catgttcccc gcgatggcgt ctcccgtgtg aagacccacc
     2041 cgtatggcga cgggagggat gccctcccgc tcgaagaccc cgtttatccc gccgatagcg
     2101 tcgatgaacc tgagcgagct ccggcaggcg ttgatctcgt ccttcggtgt ttcgagaggc
     2161 gaaccccaga aggccatgat gcagtcaccg atgtacttgt ccaccacccc tttctcatcg
     2221 atgacgacct cggtgagttc gttcaggacc cggtggagca tgagcgccgt gtcttccggc
     2281 gtatttctct ccgaaatggt ggtgaacccg gcaatgtcgg cgaagagcac ggtgaccctc
     2341 gccttcttgc cgccgggcct gacgatgtcc gggttttgga ggacatgttc aacgatggtc
     2401 ttgtccatgt actgcgaaaa ggtcctcttg atgaactgtc tttcccgtcc ctccagggcg
     2461 ta
//
LOCUS       DAOPOA010000345         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46420_length_2461_cov_3.867415, whole genome shotgun sequence.
ACCESSION   DAOPOA010000345 DAOPOA010000000
VERSION     DAOPOA010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46420_length_2461_cov_3.867415"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKJ17_07610"
     CDS             complement(<1..423)
                     /locus_tag="PKJ17_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOD75876.1"
                     /translation="MSVLSRYVGKEVRDITIMKSLHLLMVANVSIQEALRRIAEKLPD
                     SEMADRLAVASDMVSREGRTLPEAMREVGLFEKYVDLIEIGQQTGNLNTVLKELIDME
                     EELAAAKKKIISGIIYPLVVTGVSIAVGYGLTFVLGNIL"
     gene            complement(423..2249)
                     /locus_tag="PKJ17_07615"
     CDS             complement(423..2249)
                     /locus_tag="PKJ17_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOD75877.1"
                     /translation="MTNRPSMTPDACPAAGIIDRIRSLDRFHVENAISRYLVETKGMA
                     QESFGIYLDRAKHIDQSVYSVICEIENAMTYEELRTVFESLTGIPAAEVVEGRIRHVN
                     TGRLITSTIEDGRGGSSTHVYAWHPMALKDLEKEHIGKGRFFLLRKDYFHNIEVKSGF
                     SGPTDAVRAVAADTQVSRNFIYPFIRDALVHGATDIHIFPLDAQDIYRIRFRLLGDLV
                     DVHTLKAEKGRSLVNVLINWAKEMTPSLKTDEKRRPLDGKIICRKEDVGWHTDVDLRM
                     SLIWKPDGTNSDVALRVLAKTDIRNEKLQDLGYLEYHTGMFATAVSRNRGIILVTGPT
                     GSGKSRTVNTLLTGLPSTKCILCVEDPIEYQIPGGRQFQTFEYVEDAGSEKGGKASIH
                     VVGFPEFARAFKRHDPDVIFIGELRDEETVSTAVHLSKTGHLVLGTLHVSRATQIPQM
                     LVEDYGLSVDAVADNLIMGVNQVLVKKLCSACKEEMEEPLGNIPEWMKKLRYPERDLF
                     LDSIREKRFFVPSLSEGCDVCRKTIGSGGARRLLSAGYSGRTVLGEAIEFRPRHFSDG
                     KISGYHMEAKVEAADMSHSILMDAYNKMALGTIDIDAVTRLL"
     gene            complement(2246..>2461)
                     /locus_tag="PKJ17_07620"
     CDS             complement(2246..>2461)
                     /locus_tag="PKJ17_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75878.1"
                     /translation="ELPILPDIAYGGDRPGDLRVVGVIQTREVSIALTNRGELKVGSP
                     VDGDAVTAISINEVRLKSGKTFKVTAQ"
BASE COUNT          484 a          733 c          649 g          595 t
ORIGIN      
        1 gagaatgttc ccgagcacaa aggtgaggcc atacccgacc gctatcgata cgcccgtcac
       61 aaccagggga tagatgatac cggatatgat cttctttttc gccgccgcca gttcctcttc
      121 catgtcgatg agttccttga ggacggtgtt cagatttccc gtctgctgcc ctatctcgat
      181 aaggtcgaca tacttttcga aaagacccac ctcgcgcatc gcctcaggaa gcgtgcgccc
      241 ctctctgctc accatgtcgg aggcaaccgc cagccggtcc gccatttcgc tgtcaggaag
      301 tttctccgct attctgcgca atgcttcctg aatggacacg ttggcaacca tgaggaggtg
      361 aaggctcttc atgatcgtga tgtctctgac ctcttttccg acatagcgcg acagaacgct
      421 catcaaagga gcctcgtaac agcgtcaatg tctattgttc ccaaggccat tttgttgtag
      481 gcatccatga gaatgctgtg gctcatatcc gccgcctcga cctttgcctc catgtggtaa
      541 ccggatatct ttccatcgga aaaatgacgg ggcctgaatt ctatggcctc accaaggacc
      601 gtgcgacccg aataaccggc ggaaagcagg cgccgcgctc caccgctacc gatggtcttc
      661 ctgcagacgt cgcagccttc ggacagactc gggacaaaga atctcttctc acggatactg
      721 tcgaggaaga ggtctctttc gggatagcgg agtttcttca tccattcagg aatatttccc
      781 aatggttcct ccatctcttc cttgcaggcg ctgcagagtt tcttcaccag cacctggttg
      841 acacccatga tgaggttgtc cgccacggcg tcgacggaga gtccgtagtc ctccaccagc
      901 atctgcggga tctgcgtggc ccgcgatacg tggagggttc ccagcacaag gtgccccgtc
      961 ttggaaagat gcaccgccgt actgacggtt tcttcgtcgc gcaactcccc tatgaagatg
     1021 acgtccgggt catgcctctt gaaggcccgc gcaaattccg gaaaacccac cacgtgtatg
     1081 ctcgccttgc cgcccttctc gctaccagca tcctccacgt actcaaaggt ctggaactgt
     1141 cttccgccgg gtatctggta ctcgatggga tcctcgacac agagtatgca ttttgttgac
     1201 ggaaggcctg tcagaagggt attcactgtc cttgactttc cgctccctgt ggggccggtg
     1261 acgaggatga tgccccggtt gcggctgaca gccgttgcga acattcccgt atggtactcc
     1321 aggtagccta ggtcctggag cttctcattg cggatgtccg ttttggcgag gacgcggagt
     1381 gccacatcgc tgttcgtccc gtcaggcttc cagatgagcg acatcctgag atccacgtcc
     1441 gtgtgccatc ccacatcttc ctttctgcat atgatcttgc cgtccagcgg ccttcgcttc
     1501 tcgtccgtct tgagggaggg cgtcatttcc tttgcccagt tgataagcac gttgacaaga
     1561 gaacgtccct tttcggcctt taacgtgtgg acgtcgacaa ggtcgccaag gagcctgaag
     1621 cggatcctgt atatgtcctg agcgtccagg ggaaagatat ggatgtccgt ggcgccgtgg
     1681 acgagggcgt ccctgatgaa agggtagatg aaatttctcg atacctgcgt atcggccgcc
     1741 acggccctga cggcatccgt cggtcccgag aacccgctct tgacctcgat gttgtggaaa
     1801 taatcctttc ggagaagaaa gaacctgcct ttgcctatat gctccttctc aaggtctttg
     1861 agcgccatgg gatgccaggc atagacatgt gtggaagagc cgccccggcc atcttctatc
     1921 gtggacgtga tgagcctgcc ggtgttgaca tggcggatac gcccttccac cacctccgcc
     1981 gcggggatcc ccgtaaggga ttcgaagacg gtgcgaagtt cctcgtaggt cattgcattc
     2041 tctatttcgc agatgacgct gtaaacgctc tgatcgatgt gtttcgccct gtcgaggtag
     2101 ataccaaagg attcctgtgc catgcctttc gtctcgacaa gataccgcga gattgcgttc
     2161 tccacatgga agcgatcgag cgatcttatc cggtcaatta tcccggccgc cgggcacgcg
     2221 tcgggcgtca tcgagggtct attcgtcatt gtgccgtcac cttgaaggtc ttaccgctct
     2281 ttagccggac ctcgtttatc gagatcgccg tgacagcatc gccgtccacc ggggaaccca
     2341 ccttcaactc acccctgttc gtcaacgcta ttgaaacctc ccgggtctgt atgactccca
     2401 ccaccctgag gtctcctgga cgatcacctc cgtaggcgat atcgggaagg atgggaagct
     2461 c
//
LOCUS       DAOPOA010000346         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46469_length_2459_cov_3.181364, whole genome shotgun sequence.
ACCESSION   DAOPOA010000346 DAOPOA010000000
VERSION     DAOPOA010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46469_length_2459_cov_3.181364"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PKJ17_07625"
     CDS             complement(<1..532)
                     /locus_tag="PKJ17_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOD75879.1"
                     /translation="MAEKGRSTKTLNVQAVPAVVSCYTGVVKVLLVQPPIEDFYDTSI
                     RTYPLGLSYIAARVRDICDVAILDCRTGFGPRHPEDEGFPELAPYYRDDRQTPFSLFR
                     RYARYGMAGREIEAAIREARPDVVAIASSITAYSREARKVAAIAKGVDDGIITVMGGT
                     HATLFPGQLLGGGNVDY"
     gene            698..>2459
                     /locus_tag="PKJ17_07630"
     CDS             698..>2459
                     /locus_tag="PKJ17_07630"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOD75880.1"
                     /translation="MTRLRKILLWLLIAVALFTVTGFFVAPPVLKSVLIKKLSASLGR
                     TVSIGKISVNPYTLGVRFRNITVSERSGPGTFFRVDEIRARIGLSAVTGVITLGHLSL
                     RNPHVNITRYDDGSYNFSDLLEKKGTPAGETTGEKDGITFSLRDIAIQNGSADLLDTP
                     VKKKHTLREFNLSVPLLSNAPRHVERDVRPTLSFKLNDDPYVITGRTRPFADSLETFF
                     DINIENVDIPRYLAYLPVKPRFSVPSGYLDIRIEAAFRQFKDKVPLLALKGDMTVSKL
                     VVNDEKGKTVVSLPALRVAVASVEPLAGKMELSKVSIESPEFTLDRDRNGALNIVALV
                     PEMKEKQKSNGNTGKTARAGKKADGGPFQFIVDSVELTGGKVLFTDHSLKDPAKVTVE
                     KLDLKGQKISLTEGSPGAFSTSLVVNRKGAVKLEGTVALDPFSLKAKVDLKGIDIRPF
                     QPYFTDRVRVSVMSGAAGASGDLVVTQAPKKGPSVHYTGSMSVSGLSSVDKETAESLL
                     KLKSFHIRNVEFGYNPTRVTAKGVSLTDFYANIAVRPDGTLNLQQTIVKDDAEADGRI
                     GTTDNKTTAVEKGRAAPSPAG"
BASE COUNT          569 a          715 c          687 g          488 t
ORIGIN      
        1 cgtaatcgac attacctcca ccgaggagtt gcccggggaa gagggtcgcg tgggtcccgc
       61 ccatcacggt gataatccca tcatccactc ccttcgcgat ggcggcgacc ttccgggcct
      121 ctcgcgaata ggcggtgata gaagatgcta tcgccacgac atcgggacga gcctcccgga
      181 tggcggcttc tatctcacga ccggccatcc cgtaacgggc gtaccgtctg aacaacgaaa
      241 aaggcgtctg cctgtcatcg cggtagtacg gcgcaagctc cgggaatccc tcatcttcgg
      301 ggtgccgcgg gccaaaaccc gtcctgcaat caaggatggc cacatcgcat atgtcccgca
      361 cccttgcggc aatgtaagaa agtcccagcg gataggtcct gatggacgta tcgtagaagt
      421 cctcgatggg cggctggacg agaaggacct tcacgacgcc agtatagcac gaaacaacgg
      481 ctggaaccgc ttgaacgttc agagtcttgg tggatcttcc tttttcagcc attgtatatg
      541 acttcagacg cgggaacggt gacggacgag tttcccgcgt cctgcgcgtc gagcgtggaa
      601 gacgcgcacg tgacccggaa agtagtggga aaaagaggta gcgatcccac ccgcgggatg
      661 gtatgtattt gcaaggaagg ggaaaggaga atgtcggatg acaaggctaa gaaagatcct
      721 tttatggctc ctcatcgccg tcgccctttt tactgtcacg ggtttctttg tcgctccgcc
      781 ggtcctgaaa tcggtcctca tcaagaagct ttccgccagt cttggtagga cggtatccat
      841 cggcaagatc agcgtcaacc cttacacgct gggtgttcgt ttccggaaca ttaccgttag
      901 cgagcgctcc ggtcccggca cgttcttccg tgtcgacgag atacgggcca ggatcggtct
      961 ttccgctgtc accggcgtca taaccctcgg tcacctctcg ctcagaaatc ctcacgtaaa
     1021 cataacccgc tacgatgacg gatcatacaa cttctccgat cttctcgaga agaaagggac
     1081 tcccgccggg gaaaccacgg gggaaaagga tggcatcacc ttttcgcttc gcgatatagc
     1141 gatccagaac ggcagcgccg atctccttga cacgccggta aagaagaaac acaccctgag
     1201 ggaattcaat ctcagcgtcc ccctcttgtc caacgccccc aggcacgttg aaagagatgt
     1261 ccgtcccaca ctttcattca agctgaatga cgacccttac gttatcacgg gaaggacgag
     1321 gcccttcgcc gactccctgg aaacgttctt cgacatcaat atcgagaacg tggacattcc
     1381 ccgctacctt gcctatctcc cggtgaaacc ccggttctcc gtcccctcag gctatctcga
     1441 tatcagaata gaggcagcct tcaggcaatt caaggataaa gttcctcttc tcgccctcaa
     1501 gggtgatatg acggtgtcaa agctcgtcgt caacgatgag aagggcaaaa cggtcgtgag
     1561 ccttccggcc ttaagggtcg ccgtcgcgtc ggtcgagccc cttgccggaa agatggaact
     1621 ctcgaaggtc tccatcgaat cccctgagtt caccctcgat cgtgaccgga acggcgcgct
     1681 gaatatcgtg gcccttgtac cggaaatgaa ggagaagcag aaaagcaacg gcaacacggg
     1741 aaagacagct agggccggga agaaagccga cggcggtcct tttcagttca tcgtcgatag
     1801 tgtcgagctc acgggaggaa aggttctctt taccgaccat tctctcaagg atccggcgaa
     1861 ggtaaccgtc gagaagctgg acctgaaggg tcagaagatc tcgctcaccg agggctctcc
     1921 cggtgcgttc tccacatctc tcgtcgtcaa caggaaaggg gccgtgaaac tggaagggac
     1981 ggtggcattg gacccgttct ccttaaaggc gaaggtggac ctcaagggca ttgatatccg
     2041 cccctttcaa ccctatttca ccgacagggt cagggtatcg gtgatgagcg gtgcggcggg
     2101 cgcgagtgga gacctcgtag taacacaggc gccaaagaag gggccttccg tgcattacac
     2161 aggaagcatg tccgtctcgg gcctctcatc cgtcgacaag gagacggcgg agagtctgct
     2221 caagctgaag tctttccaca tccgaaacgt cgaattcgga tacaacccta cccgggttac
     2281 cgcaaaagga gtatcgctca ccgactttta cgccaatatt gctgtcagac ccgacgggac
     2341 gctgaacctc cagcagacga tcgtcaagga tgatgcggag gcggacggcc gcatcggaac
     2401 gaccgacaac aaaacgacgg ccgtggagaa ggggcgtgcg gctccttccc ctgccgggg
//
LOCUS       DAOPOA010000347         2446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46758_length_2446_cov_4.083229, whole genome shotgun sequence.
ACCESSION   DAOPOA010000347 DAOPOA010000000
VERSION     DAOPOA010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46758_length_2446_cov_4.083229"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..1133)
                     /locus_tag="PKJ17_07635"
     CDS             complement(294..1133)
                     /locus_tag="PKJ17_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOD75881.1"
                     /translation="MDTRTPTVLALIASPRILGNCETFAKEVSRNIPEAHRLRLLRLT
                     SLKIGRCRACYGCVVGRKCPVEDDMAFLLGEIANADAFIIASPVYYLGANASIKAVLD
                     RGFLFFDILEKTHGKPAVLLNFYGMPGRIGAAPQTLETLAAFLGLSVKASLSIEAALP
                     GEALMNKANAGRATRLGKALFSRGKRRRAGGCPFCGCEIIRVVPTGFVCTVCHGSFTI
                     GPQGRFVKGKDGGILGSPEHILLHREWLRGMKERFNDRKREIMKTIIPYRDDGEWVEP
                     PGK"
     gene            1419..>2446
                     /locus_tag="PKJ17_07640"
     CDS             1419..>2446
                     /locus_tag="PKJ17_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HOD75882.1"
                     /translation="MLVIILLGLFIFQIYNKPKKNLESIAFSDFSEAVRADKVASVLM
                     QGRNITGTYKDGKEFKTFAPEDPDLVKTLREKNVKITAKPDEESGLWQSIFISWFPML
                     LLIGVWIFFMRQMQSGGGKAMAFGKSKARLFTGRENRVTFHDVAGIDEAKEELQEVID
                     FLVDPKKFTKLGGRIPKGVLLVGPPGTGKTLLARAIAGEANVPFFSISGSDFVEMFVG
                     VGASRVRDLFNQAKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGF
                     ESNEGVIVMSATNRPDVLDTALLRPGRFDRQIVVPTPDVKGRADILKVHTRKTLVDEN
                     VDLSIIARG"
BASE COUNT          521 a          733 c          705 g          487 t
ORIGIN      
        1 cggaacttca cgggctacaa cggatacggc aaccctgcag tcagcgtggg cacgggtaat
       61 gccgaccgca caggcgtggc cgatgtttac agattcggcg cctggtactt cttctaagaa
      121 gcacattacc gtacacagga aaggggcctt cgggcccctt tttttgcttg aaccgcttga
      181 accgcttgaa ccgtttcaac cgctcggaaa ggcaagaata ttagggaacg ttcgacgagc
      241 gagcatttcg ccctttcttt gaacgttcaa gcggttcaag cggttgcaac catctacttc
      301 cccggtggct ccacccactc accgtcgtcc ctgtaaggga tgatggtctt catgatctct
      361 cttttcctgt cgttgaagcg ttccttcatg ccccgcagcc attcgcggtg aaggagtatg
      421 tgttcgggag agcccaggat accaccatcc ttccctttga cgaaccggcc ttgcggcccg
      481 atggtgaaag aaccgtggca aacggtgcag acgaagcctg tcgggacaac gcggatgatc
      541 tcgcagccgc agaaggggca cccccctgcc ctccttcgct tcccccgtga gaagagcgcc
      601 tttccaaggc gcgtggcccg tccggcgttg gccttgttca tgagggcctc gccggggagc
      661 gccgcctcga tggaaagact cgccttgacg gacagcccga gaaaggccgc aagggtctcg
      721 agcgtctggg gcgccgctcc gatccgcccc ggcattccgt agaaattgag aaggaccgcc
      781 ggtttgccgt gggtcttctc gaggatgtcg aagaagagga accccctgtc gagaacggcc
      841 ttgatgctgg cgttcgcccc gaggtaatag acaggggagg cgatgatgaa ggcgtcggcg
      901 ttcgcgatct caccgaggag gaaggccatg tcgtcctcca ccggacactt cctgcccacg
      961 acacatccgt agcaggcccg gcaccttcct atcttcagcg atgtgagcct caggagcctc
     1021 aacctgtgcg cttccgggat gttccgggac acctccttgg cgaaggtttc gcaatttccg
     1081 aggatcctcg gtgaagcgat gagcgcaaga actgtcggtg ttctggtatc catagcatcc
     1141 tggtccctcc ctgcgacccg tatcgttctt attgcacacc agggcgtcaa aagcaaggga
     1201 gaaagaaggg atcagggggc aggccccggg aggcgcatga cctcccgtgc cttgaaatgc
     1261 aaaggttttt attgtctttt ccccgttccc ttaaccccaa gacctgaaac ccgactgtta
     1321 atgcctgaca atattgaaaa ttatggcctt cttgtggtac actctgacct atgtcaaaag
     1381 ctagcggaca aggacccaca aacaagagca tcatcaccgt gctggtgatc atattgctcg
     1441 gcctcttcat cttccagatc tacaataagc cgaagaagaa tctggaaagc atagccttca
     1501 gcgatttctc ggaggcggtg cgcgccgaca aggtcgcgtc cgtcctgatg cagggacgta
     1561 acatcaccgg aacgtacaag gacggcaaag agttcaagac ctttgcgccc gaggacccgg
     1621 atctcgtgaa gacgctccgg gaaaagaatg tgaagatcac cgcgaaaccc gatgaggaat
     1681 ccggattgtg gcaatccatc ttcatatcct ggttccccat gctcctgctc atcggcgtct
     1741 ggatcttctt catgcgccag atgcaatcgg gaggcggcaa ggccatggcc ttcggcaaga
     1801 gcaaggcacg gctcttcacc ggccgcgaga acagggtgac ctttcacgat gtggcgggga
     1861 tcgacgaggc aaaggaagag ctccaggagg tcatcgactt cctcgtggac ccgaagaaat
     1921 tcacgaaact tgggggacgc atccccaagg gggtccttct cgtggggcct cccggcaccg
     1981 gcaagacact ccttgcccgc gccatagcgg gtgaggccaa cgtgcccttt ttcagcataa
     2041 gcgggtccga tttcgtcgag atgttcgtcg gcgtcggcgc ctcgcgcgtc cgcgacctct
     2101 tcaaccaggc gaagaagaat gccccctgca ttattttcat cgacgagatc gatgccgtgg
     2161 ggagacaccg cggggcgggg ctcggaggcg gccacgacga gcgggaacag acactgaacc
     2221 agctcctcgt ggagatggac ggtttcgagt ccaatgaagg cgtcatcgtc atgtccgcca
     2281 cgaacagacc cgacgtcctc gatacggccc tgcttcgtcc cggccgtttc gacaggcaga
     2341 tcgtcgtgcc cactcctgac gtcaagggca gggcggatat cctcaaggtc cacacacgca
     2401 agaccctcgt cgacgagaac gtcgacctct cgatcatcgc gcgggg
//
LOCUS       DAOPOA010000348         2439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46953_length_2439_cov_2.086829, whole genome shotgun sequence.
ACCESSION   DAOPOA010000348 DAOPOA010000000
VERSION     DAOPOA010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46953_length_2439_cov_2.086829"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PKJ17_07645"
     CDS             <1..830
                     /locus_tag="PKJ17_07645"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HOD75883.1"
                     /translation="ARKVEMQNTERRLSQKELNIDKKLDALEKKEEELSRKDREMSNR
                     YNHLEGRIREQETLVARSKEQLEKISGMTGEEAKKMLVTAMEEEASYESMKMIKKIEE
                     EAREKADKKAKEIIAFAIQRYAGEYVGEDTVSVVPLPNEEMKGRIIGREGRNIRALEA
                     ATGVDIIIDDTPEEVILSCFNPVRREVARIAIAKLISDGRIHPARIEEIVSKVAEEME
                     EKIKEAGEQAVFDLGIHNVHPELVKLLEDSSSAAVTPRTSTSTLWRWPSSAGPLPRN"
     gene            794..1330
                     /locus_tag="PKJ17_07650"
     CDS             794..1330
                     /locus_tag="PKJ17_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOD75884.1"
                     /translation="MAFICGAIASELKVSVKEAKRAGLLHDIGKAVDHEIEGSHATIG
                     ADLARKYGESDEIIHSILAHHEDVEVKSLLDVIVQAADALSGARPGARREMLETYIKR
                     LQELEKIANTFQGVEKSYAIQAGREIRIVVNSERVSDENIIMMSKDIAKKIETDLSYP
                     GQIKVVVIRETRSIEYAK"
     gene            1320..2099
                     /locus_tag="PKJ17_07655"
     CDS             1320..2099
                     /locus_tag="PKJ17_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOD75885.1"
                     /translation="MQNDRFTVLFLGDVIGKPGRKAVGRYLEETRADFVVINGENLAG
                     GIGITARTAVEMLSLGVDAITTGNHVWKKKEVVPFLMEEKRVVRPLNYPRGTPGFGYT
                     VVKKNDLSLCVANIEGRIFMNSLDCPFRAMEEFLEMERPDAPIIVDFHAEATSEKIAM
                     GWYLDGKVTALMGTHTHVQTADDRVLPRGTGYITDAGMTGPADSVIGMDKRGVLERFY
                     TQMPQKFEVGKDDIEVQGVLLTIDRNGHRCLDIKRVKEKVA"
     gene            2149..>2439
                     /locus_tag="PKJ17_07660"
     CDS             2149..>2439
                     /locus_tag="PKJ17_07660"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567413.1"
                     /note="catalyzes the formation of tyrosyl-tRNA(Tyr) from
                     tyrosine and tRNA(Tyr); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOD75886.1"
                     /translation="MGIEEQLAVIKRGAVDLINEKELRDKLVRSEKEGRPLRVKLGLD
                     PTAPDLHLGHTVVLQKLKQFQDLGHIAIFLIGDFTGMIGDPTGRSETRPALTK"
BASE COUNT          640 a          610 c          769 g          420 t
ORIGIN      
        1 aggcgcgcaa ggtggagatg cagaacaccg agcgcaggct ttcccagaaa gagctcaata
       61 tagataaaaa actcgatgcc ctcgaaaaga aagaggaaga actgtcgcgg aaggaccgcg
      121 agatgtcgaa caggtacaat cacctggaag gcaggatacg agagcaggag accctggttg
      181 cccggtcgaa ggagcagctc gagaagatat cgggcatgac gggcgaagag gcaaagaaga
      241 tgctcgtgac ggccatggag gaagaggcca gctacgaatc gatgaagatg atcaaaaaga
      301 tcgaagagga agccagggag aaggcggaca agaaggcgaa ggagatcata gcttttgcca
      361 tccagaggta tgccggtgaa tacgtcggcg aggataccgt ttccgtggtt cccctgccga
      421 acgaggagat gaagggcagg atcataggtc gtgaggggcg taacatcagg gccctggagg
      481 ccgcgacggg cgtggatatc atcatcgacg acacccccga agaggtcatt ctttcctgct
      541 tcaaccccgt acggagggag gtcgcaagga tcgccatcgc aaagctcata agcgacggca
      601 ggatacaccc cgcccggatc gaggagatcg tctccaaggt tgccgaggag atggaggaga
      661 agatcaagga ggccggcgaa caggcggtgt tcgacctcgg gatccacaac gttcaccccg
      721 agctcgtcaa gcttctggaa gactcaagtt ccgcagcagt tacgcccaga acatctacca
      781 gcactctttg gaggtggcct tcatctgcgg ggccattgcc tcggaactga aggtgagcgt
      841 caaggaggcg aagagggcgg ggctccttca cgatatcgga aaggccgtcg accacgagat
      901 cgagggctcc catgcgacga taggggctga cctggcaaga aagtacgggg agagcgacga
      961 gatcatccat tccattctcg cccaccacga ggacgttgag gtcaaaagcc tcctcgatgt
     1021 gatcgttcag gccgccgacg ccctttccgg cgcgaggccg ggagcccgca gggagatgct
     1081 ggaaacctat atcaagaggc tccaggaact ggaaaagatc gccaatacct tccagggtgt
     1141 tgaaaagtcc tatgccatac aggcgggcag agagatacgc atcgtcgtga acagcgagag
     1201 ggtaagcgac gagaacatca taatgatgtc gaaggacatc gcgaagaaga tcgagaccga
     1261 tctgtcctat ccaggccaga tcaaggtcgt cgtgatcagg gagacacgtt cgatagaata
     1321 tgcaaaatga ccggtttaca gtgctcttcc tgggtgacgt gatcggaaag ccgggaagga
     1381 aggccgttgg ccgttatctg gaagagacgc gcgcggattt tgttgtgata aacggcgaga
     1441 accttgcggg tgggataggc ataaccgcca ggacggcggt ggagatgctt tcccttggcg
     1501 tcgatgccat aactacgggc aaccatgtgt ggaagaagaa agaggtggtg cccttcctta
     1561 tggaagagaa aagggttgtc cgtcccctca actatccccg ggggactccc ggcttcggat
     1621 ataccgttgt gaagaagaat gatctttccc tgtgcgtcgc caacatagag ggacgcatct
     1681 tcatgaattc tctcgactgt cccttcaggg ccatggagga gttcctggag atggaaagac
     1741 cggatgctcc gatcatcgtc gattttcacg cggaggcgac gtccgaaaag atagccatgg
     1801 gatggtacct cgacggaaag gtgacggccc tgatgggcac gcatacgcac gttcagaccg
     1861 cggacgacag ggtgctgccg agaggcaccg ggtacatcac cgacgcgggc atgacgggcc
     1921 ccgccgattc ggtcatcgga atggataaaa ggggggtcct tgaacggttc tatacccaga
     1981 tgcctcagaa gttcgaggtg ggaaaggacg atatcgaggt ccagggggtc ttgctcacca
     2041 tcgacaggaa cgggcacaga tgcctcgata tcaaaagggt caaggaaaaa gtagcctgaa
     2101 tccgtcacgc agttcagatc ttctaaaaac atcatactgg agacgtcaat ggggatcgaa
     2161 gagcagttag cagtcatcaa gcgcggtgcg gtggacctca tcaacgagaa ggagctcagg
     2221 gacaagctcg tgcgctcgga gaaggaagga aggccgctga gagtcaagct tggtctcgac
     2281 ccgacggcac ccgaccttca tctcggtcat acggtggttc tccagaagct gaagcaattt
     2341 caggacctgg gccacatcgc catattcctt atcggtgatt tcacggggat gatcggcgac
     2401 ccgacgggac gcagcgaaac acgccccgcc ctcacgaag
//
LOCUS       DAOPOA010000349         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_46966_length_2438_cov_2.898867, whole genome shotgun sequence.
ACCESSION   DAOPOA010000349 DAOPOA010000000
VERSION     DAOPOA010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46966_length_2438_cov_2.898867"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="PKJ17_07665"
     CDS             complement(<1..1625)
                     /locus_tag="PKJ17_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75887.1"
                     /translation="MKRFRSYRLMMLLTAAAVMFVLSPAQREALAFSAVQNLDLDTHG
                     IILRGAWEQLSKDPVFKDSNFPRIDGISRFEGVFKKASDPFATGGGGPDVKGRTRDSE
                     HYYNPKITGKNKGGAPASVGKYYSILVERMLGGQGGVSPEQGAAWAAHFLADMNVPYH
                     IVGIPAGGAEAIYVGGIPGRTAHSLTSDESGPLWLKEVPLTYDRLWKAFTFDFYRDWP
                     GWGTTQDFERTIAVFHTVRERAGSERDLHDWFDPWYYNGWGMGMEPFQTNPVVHNSSH
                     AKWEVWAHLYLARWTNYKPSVNTGFHPQWRNGWVTFNDKVPWEAQKAQAEEFAKKAAE
                     QTWDEMQQVYRSPDIATDDAVWRVMTLWRASISAMKPSMEARQDLTNPARHELRAIID
                     NKEKENVFDVKVRLTLTGDGSTFSEVKDITGSIAAAGKSEVTFTIEPKDPAKCKVRLE
                     VIGHYEKTPDLQYARIEEPFGGIRRSADEAQNTALRGILDSLKQAAEKAERLSTEIAA
                     LCSASSTAIEAAQADLKKLGARVESMEERVKST"
     gene            1863..>2438
                     /locus_tag="PKJ17_07670"
     CDS             1863..>2438
                     /locus_tag="PKJ17_07670"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOD75888.1"
                     /translation="MKNVRMALAGLMAGLMLVLAIEAHAADPDTVYIRHLEGIVELAE
                     AGSPQWMEAAVNTPLIAGDTVRTAASGKAELFLKDGSVVRVGKNSVMRIVAVEPKGVQ
                     FRLDRGGAYIVARGSKDVPIFFDTPSAALDVTQPTTVRVDVYDGGISEVSVYQGEVQA
                     LQQKGRMPVRAGERLALAADGSIPRVTGLRSA"
BASE COUNT          448 a          717 c          711 g          562 t
ORIGIN      
        1 tctgtggact tgacccgttc ttccatggat tcgacgcggg ctccaagctt cttgagatcg
       61 gcctgcgccg cttctatggc cgttgatgag gcgctgcaca gcgcggcgat ctccgtcgat
      121 aggcgctcgg ccttttccgc cgcctgtttc aggctgtcaa ggattccccg cagagcggtg
      181 ttctgagctt catcggcgga cctgcgtatg cccccgaagg gctcttcgat cctggcgtac
      241 tggaggtcgg gggtcttttc atagtgaccg atcacctcga gacggacctt gcatttcgcc
      301 gggtctttcg gctcgatggt gaaggtcact tcgctctttc ccgcagccgc gatgctcccc
      361 gtgatgtcct tcacttcgct gaaggtgctg ccgtcaccgg tcaatgtgag ccgcaccttt
      421 acatcgaaga cgttctcttt ttccttgttg tcaatgatcg cccggagttc atgccgcgcg
      481 gggttcgtca ggtcctgcct tgcttccatg gacggtttca tcgcagatat ggatgcccgc
      541 cacagtgtca ttacgcgcca tacggcgtca tccgttgcta tatcgggcga ccggtatacc
      601 tgctgcatct cgtcccaggt ctgctccgcc gcttttttcg cgaactcttc agcctgtgcc
      661 ttctgggctt cccagggcac cttgtcattg aaagtcaccc atccgttcct ccactgagga
      721 tgaaatcccg tattgacgga gggcttgtag ttggtccacc gggcgaggta gaggtgggcc
      781 cagacctccc acttcgcatg actcgaattg tgaacgacag ggttcgtctg gaaaggttcc
      841 atgcccatcc cccagccgtt gtaataccag gggtcgaacc agtcgtgaag gtccctttcg
      901 gagccggcac gttccctcac cgtgtggaag acggcgatgg tccgttcgaa atcctgtgtc
      961 gttccccatc ccggccagtc ccggtagaaa tcaaaagtga aggccttcca cagcctgtcg
     1021 taggtaagag gtacctcctt gagccacagc ggaccggact cgtcagacgt gaggctgtgg
     1081 gcggtccttc cggggatgcc tcccacgtag attgcctcgg cccctcccgc gggtatgccg
     1141 acaatatggt agggaacatt catgtccgcg agaaaatgtg cggcccaggc ggcgccctgt
     1201 tcgggggaaa cccctccctg tccgccgagc attctctcca caaggatcga gtaatacttg
     1261 ccgaccgatg ccggagcccc gcccttgttc tttcccgtga tcttcggatt gtaatagtgt
     1321 tctgagtccc tcgtgcgccc cttgacgtca gggcctcctc cgccggttgc gaaaggatcc
     1381 gacgctttct taaaaacccc ttcgaacctg gagatcccat cgatccgggg aaagttgctg
     1441 tctttgaaga cggggtcctt tgacaattgc tcccacgctc cgcgtaggat gatcccgtgg
     1501 gtgtcaaggt cgaggttctg aacggcgctg aacgccagtg cctcacgctg ggccggcgac
     1561 aggacgaaca tcacagccgc agcggtgaga agcatcatga gccgatacga tctgaacctt
     1621 ttcacaaggg cctccgaagc attttcctcc aattcttacg ttttatggca tttttttcaa
     1681 cccaaaacgg cctcccttgt cttcgacggt attgtcgttg ccagaaggtc ccgtcgacag
     1741 atatttcccc tcctgaggca gtatcgtcaa taaaatgaca atagttataa tgggttaaag
     1801 cgaatcaacc tgctggtacg catcttgctc tcaagaaggg atcaaagttg aggagagggc
     1861 atatgaaaaa cgtacgtatg gcactagcgg gattgatggc gggattgatg cttgtccttg
     1921 ccattgaggc ccatgcggct gaccctgaca ccgtatacat acgacacctc gaaggcatcg
     1981 tggagcttgc agaggccggg tcaccgcaat ggatggaggc tgccgtgaat acgcctctca
     2041 tcgcgggcga caccgtccgg acggcggcgt cggggaaggc cgagctcttc ctgaaggacg
     2101 ggagcgtggt tcgtgtgggg aagaactcgg ttatgaggat cgtcgccgtg gaaccgaagg
     2161 gcgttcagtt caggcttgac cggggagggg cgtacatcgt cgcccgtggg tcaaaggatg
     2221 ttccgatctt cttcgatacc ccgtcagcag cgctcgacgt cacgcagcct acgacggtgc
     2281 gggtggatgt atacgacggt gggatcagcg aggtgtccgt ctatcagggt gaagtgcagg
     2341 cgctgcagca gaagggccga atgcccgtca gagccgggga gcgtctcgcc ctcgccgcgg
     2401 acggatccat tccccgtgtc accgggctgc gctctgcc
//
LOCUS       DAOPOA010000350         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_47044_length_2435_cov_3.676891, whole genome shotgun sequence.
ACCESSION   DAOPOA010000350 DAOPOA010000000
VERSION     DAOPOA010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47044_length_2435_cov_3.676891"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKJ17_07675"
     CDS             complement(<1..204)
                     /locus_tag="PKJ17_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOD75889.1"
                     /translation="MLTKRIIPCLDVMEGRVVKGTNFLELKDAGDPVENAKAYEEQLA
                     DELCFLDITASHEKRRTIIDVVER"
     gene            complement(194..901)
                     /locus_tag="PKJ17_07680"
     CDS             complement(194..901)
                     /locus_tag="PKJ17_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HOD75890.1"
                     /translation="MKALFAMDLMGGKTVRLRKGDFQAVTVYSEDPPSLIEEMVHRGA
                     RDFHIIDLDGARTGEPVHGEIIKRIRSTVKGYMEVGGGIRTDDTIKYYSDLGIDGIIV
                     GTRALEDEEYFEGLSRFPNIVLGLDLLGGKPMSRGWKSAVDRDPATILKAAERIGIMA
                     VLCTSIERDGMLTGPDLTGLTAISGMTRLPVIASGGVSKIDDVRRLAEMDVWAAIIGK
                     AFYEGFIGIEEAMGYAD"
     gene            complement(898..1464)
                     /locus_tag="PKJ17_07685"
     CDS             complement(898..1464)
                     /locus_tag="PKJ17_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75891.1"
                     /translation="MFMTLARAWKLCFIGLFVLVFGVSSVHAVGDLGIVVEKLKDPEK
                     TLFCPVCGRAISPGVVHTEATAIIREKLKTALTDRNIGYSDGMKQDQPYIQVLVYRFQ
                     ERKGGNFAVEKPASVAFHMHLMKNGVVGKIFAYSEEQKALMQNLLTVGKFFSRGARWV
                     TAEELAGEGINAGLDELVEPKETTEPAE"
     gene            complement(1684..2373)
                     /locus_tag="PKJ17_07690"
     CDS             complement(1684..2373)
                     /locus_tag="PKJ17_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD75892.1"
                     /translation="MLDVNAIDTYYGLSHILFGVTLSVRAGEVVGLLGRNGAGKSTTM
                     KSIMGIVPPRKGTITFKGAGIAGRKPYQLFRQGIGYVPDDRRVFADLCVDDNLEIVHR
                     RNDEWDKERVYGLFPALKEIRSRRAGHLSGGEQQMLTIARALMGSPELLLLDEPTEGL
                     APLIVRDLEEQILRLKEAGISILLSEQNVRSALKMITRAYVIDNGRIRFEGTVQELEA
                     NEEVKRKYLMI"
BASE COUNT          473 a          765 c          669 g          528 t
ORIGIN      
        1 ccgctcgacg acgtcgatga tggtccgtct tttctcatgg gaagcggtga tgtcgagaaa
       61 gcacagctcg tccgccagct gctcctcgta tgcctttgcg ttctccacag gatcgccggc
      121 gtctttgagc tcgagaaaat tggtcccttt tacgaccctg ccctccatga cgtcaaggca
      181 gggaatgatc cttttagtca gcataaccca tggcctcctc gatgcctatg aacccctcgt
      241 agaaggcctt ccctatgatc gccgcccaga cgtccatctc cgcgaggcgc ctgacgtcgt
      301 ctatcttcga cacgccgccg ctcgctatga cgggcagacg tgtcattccc gatattgccg
      361 tgaggccggt cagatcaggt ccggtcagca tcccgtcgcg ctcgatgctg gtgcacagga
      421 cggccatgat cccgatgcgc tcggcggctt tgaggatggt tgcggggtcc ctgtcgacgg
      481 cgctcttcca tccccgcgac atcggcttgc ccccgagaag gtcgaggccg aggacgatgt
      541 tggggaaccg tgacaaccct tcgaaatact cctcatcctc cagggcgcgg gtgccgacga
      601 tgatgccgtc gatgccgaga tcgctgtagt atttaatggt atcgtcggtg cggatgccgc
      661 cgccgacctc catgtatccc ttcaccgtgg aacggatcct tttgatgatc tccccgtgca
      721 cgggctcgcc cgtccttgcg ccatcgagat cgatgatatg gaaatcccgg gcgccgcggt
      781 ggaccatctc ctcgatcagc gagggcgggt cttcgctgta aaccgtcacc gcctggaaat
      841 cacccttcct gagacgcacg gtcttgccgc ccatcaggtc catcgcaaag agcgctttca
      901 ctccgcgggc tccgtcgtct ccttcggctc gacgagttcg tccagtccgg cgttgatgcc
      961 ttccccggca agctcttccg ccgtcaccca gcgcgccccc ctgctaaaga acttccccac
     1021 tgtcagcagg ttctgcatga gggccttttg ctcctctgag taagcgaata tcttgcccac
     1081 caccccgttc ttcatgaggt gcatatgaaa ggcaacggag gcgggttttt ccacggcgaa
     1141 attaccaccc ttgcgctcct ggaaacgata gacaaggacc tgtatgtaag gctggtcctg
     1201 cttcatcccg tccgagtatc cgatgttccg gtccgtgagc gcagtcttga gcttctccct
     1261 gatgatggcc gtggcctcag tatgaacgac accggggctg atcgccctgc cgcacacggg
     1321 gcagaacagg gttttctccg gatccttgag cttctccacg acgatgccga ggtcgcccac
     1381 ggcatggacg gacgaaacgc caaagacgag cacgaacaac ccgatgaaac aaagcttcca
     1441 ggctctcgcc aaagtcatga acatgtataa attctatagg aaaaccggac caaaagaaag
     1501 acaaaaagag agtgaaaacg ggctcaggtc tcacgtttca ggtttcaggc caaagaccgg
     1561 gaaacatatc cgcctttttt cggttctcac ctgaaaggtg aaacctgaaa cggtcttttt
     1621 gtcttttgct taaaacctaa aacttaaaac ctaaaacgga tctggttttc gcggagccga
     1681 aaactagatc ataaggtact tccgtttcac ctcttcattc gcttccagtt cctgaacggt
     1741 gccttcgaag cggatcctgc cgttatcgat gacgtaggcc ctggtgatca tcttcaaggc
     1801 cgagcggacg ttctgctccg acaggaggat gctgatgccg gcctctttga gcctcaggat
     1861 ctgctcttcc aggtcccgga cgatgagcgg ggcgaggccc tccgtgggct cgtcgaggag
     1921 caggagctcg gggctcccca tgagcgcccg cgcgatggtg agcatctgct gctccccgcc
     1981 ggagaggtgc ccggcgcgac gcgaccttat ttccttcagg gcggggaaaa gaccgtacac
     2041 cctttccttg tcccactcgt cgttgcgccg gtggactatc tccaggttgt cgtcgacgca
     2101 caggtcggcg aagaccctgc ggtcatcggg gacatacccg ataccctgac gaaaaagctg
     2161 gtaaggtttg cgcccggcga tcccggcccc cttgaaggtg atcgtcccct tccgcggcgg
     2221 cacgattccc atgatgctct tcatggtggt cgacttcccc gcaccgttgc gaccgagaag
     2281 gccgacgact tcgccggccc tgacactcaa ggtcacgccg aagaggatat ggctcaatcc
     2341 gtagtaggtg tcgatggcgt tcacgtcaag catgcgtcgc tcccgccgag atatgcgtcc
     2401 tgcacctcct ggcagcacct gacgtcgtcg caggt
//
LOCUS       DAOPOA010000351         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_47162_length_2431_cov_3.682239, whole genome shotgun sequence.
ACCESSION   DAOPOA010000351 DAOPOA010000000
VERSION     DAOPOA010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47162_length_2431_cov_3.682239"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1984)
                     /locus_tag="PKJ17_07695"
     CDS             complement(41..1984)
                     /locus_tag="PKJ17_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD75893.1"
                     /translation="MLKNKGIAFKLVLFFTLSSAAIFAAIFSYNYLVSRRMIQKGIEE
                     NSANLITTTTSRIEVLLTSTQKVPQNTAYLLENTNYDEAELFKVIYAIVEKNPEIYGA
                     AIAFEPYAFDRTKKHFAPYFYRNGEGISFKYLDRFYDYFHWDWYQIPRELEEPQWTEP
                     YFGEGGGILMSSYAVPFYRVVDGTRRLTGVVVVDISLEELRKIVSSIRILKSGYGFLI
                     SKNGTFVTHPIKEHIMNETIFSVAEERKEPTLRETGRRMIRGERGYSATPVKSAVTGK
                     SCIMTYVPIKSNGWSLGVLFPYDELMADITNLNHVVLALGITGLLLLALAVVFIARSI
                     TKPLTEMADIAGRIGTGDLEAQVPMVRTKDEVGKLASALDCMKTSLKDYIREITDTTA
                     ARQRMESELKIARNIQMGILPKVFPPFPDRKDFDIHALTIPAREVGGDFYDFFLIDRD
                     HLCIVIGDASGKGIPAAIFMAFTKTLIKAKASVSVQTGTLMEEVNDELSRGNPADMFI
                     TVFCAILNTATGELRYTNGGHNPPVIIRRTGDLSYLDGPGELVVGIMEGTQYTTRTLA
                     LNPGDGLFLYTDGVTEAMNAAGEIFSTDRLKDVLATCADGDVSAITACVGGAINDFCK
                     DAPQYDDITMMAVRYEGTGSGNG"
     gene            complement(1971..>2431)
                     /locus_tag="PKJ17_07700"
     CDS             complement(1971..>2431)
                     /locus_tag="PKJ17_07700"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD75894.1"
                     /translation="MWYNEYHTVLNAGINADELTSFFYSDYGLNFPEDGIYIMEETLK
                     KDPAAVCAFVSASLEGWRWAFDHPEEAIDIILKYMVEANVPANKVQQRWMLERMRDVI
                     TPSGSDRAAMGVLKKEDYERVGKELKDGGLIGDVPGMDAFAFTCTGYVKK"
BASE COUNT          477 a          727 c          665 g          562 t
ORIGIN      
        1 ggaaaccgcc tcccccccat cacccattac ctatctccca tcacccgttc ccactgcccg
       61 tcccctcata tctcaccgcc atcatggtga tatcgtcgta ttgcggcgca tccttgcaga
      121 aatcgttgat ggcccctccg acacaggccg tgatcgccga gacatcccca tcggcgcatg
      181 tggcgagcac atccttcagg cgatccgtcg agaatatctc acccgcagcg ttcatcgctt
      241 ccgtgacacc gtcggtgtac aggaacaacc cgtcacccgg gttgagcgca agggtcctcg
      301 tggtgtattg cgttccttcc atgatcccga cgacgagttc gccgggaccg tcgagatagc
      361 tgagatcgcc ggtgcggcga atgatgacag gcggattgtg gccgccgttc gtataccgga
      421 gctcgcccgt ggccgtgttc aggatggcgc agaacacggt gatgaacatg tccgcggggt
      481 tccctctgct cagttcgtca ttgacctcct ccatgagtgt acctgtctgg acgctgaccg
      541 aggccttcgc cttgataagg gttttcgtga acgccatgaa gatggctgcc ggtattccct
      601 tgccggaggc gtcgcctatc acgatgcaga ggtggtccct gtctatgagg aagaaatcgt
      661 agaagtcccc gcccacctcg cgggccggta tggtaagggc atggatatcg aaatccttcc
      721 tgtcaggaaa gggagggaac accttgggaa ggatgcccat ctggatgttc ctggctatct
      781 tgagctcgct ctccatgcgc tggcgcgcgg ccgtcgtatc ggtgatctcc cggatgtagt
      841 ccttgagcga cgtcttcatg caatcgaggg ccgatgcgag ctttcccacc tcgtccttcg
      901 tccgcaccat gggtacctgg gcctcgaggt cgcccgtgcc tatcctcccc gctatgtcag
      961 ccatctccgt cagcggcttc gttatggagc gggcgatgaa tacgaccgcg agggcgagaa
     1021 gcaaaaggcc ggtaataccc agcgcgagga ccacatgatt gagattggtg atgtccgcca
     1081 taagctcgtc gtagggaaag agaacgccca gggaccaccc gttcgacttg atcgggacat
     1141 aggtcatgat gcatgacttc cccgtcaccg cgctcttcac aggggtcgcg gaataacccc
     1201 tctccccccg tatcatcctc cgtcccgttt cgcgcagcgt gggttccttc ctctcttccg
     1261 cgacggagaa gatcgtctcg ttcatgatgt gctctttgat gggatgggtg acaaatgtgc
     1321 cgttcttcga tatgagaaac ccatacccgg acttcaggat ccttatcgag gagacgatct
     1381 ttctgagttc ctcgagggag atgtccacga cgaccacacc ggtcagcctc cgcgttccgt
     1441 cgacaaccct gtagaaaggc acggcatagg atgacatgag gatgccgccg ccctcgccga
     1501 aatagggttc cgtccattgc ggctcttcga gctcccgggg gatctgatac cagtcccagt
     1561 gaaaatagtc atagaacctg tcgagatatt tgaagcttat cccctcgccg ttcctgtaga
     1621 aatagggagc gaagtgcttc tttgtcctgt cgaaggcgta gggttcgaag gctatcgccg
     1681 cgccgtatat ctccgggttc ttctccacga tggcatagat gaccttgaaa agctcggcct
     1741 catcgtaatt cgtgttctcg agaagatatg ccgtgttctg gggcaccttc tgtgtggatg
     1801 tcaggagaac ctcgatcctg ctcgtcgtgg tggtgataag gttcgccgag ttctcttcga
     1861 tgcccttctg gatcatcctg cgggagacga ggtaattgta gctgaatatg gcggcaaaga
     1921 tggcggcgct gcttaaggtg aaaaaaagga ccagtttgaa ggcgatgcct ttatttttta
     1981 acatatcccg tacacgtaaa agcaaaggcg tccatgccgg gcacgtcccc gataagaccg
     2041 ccatccttca gctctttccc caccctttcg taatcttcct tcttgaggac acccatcgcc
     2101 gccctgtcac tgccggaggg agtgatcacg tccctcatcc tttcgagcat ccatcgctgc
     2161 tgcaccttgt tggccggcac gttcgcctcg accatgtact tgaggatgat atcgatggcc
     2221 tcctccgggt ggtcgaaggc ccagcgccac ccctcgagag acgcgctcac aaaggcgcat
     2281 accgccgcag ggtccttctt cagcgtctcc tccatgatgt agatcccgtc ctctggaaag
     2341 ttcaggccgt aatcgctgta aaagaaggat gtcagctcgt cggcgttgat cccggcattg
     2401 agaaccgtgt gatactcgtt gtaccacatg g
//
LOCUS       DAOPOA010000352         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_47307_length_2426_cov_4.328132, whole genome shotgun sequence.
ACCESSION   DAOPOA010000352 DAOPOA010000000
VERSION     DAOPOA010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47307_length_2426_cov_4.328132"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..318)
                     /gene="rpmB"
                     /locus_tag="PKJ17_07705"
     CDS             complement(127..318)
                     /gene="rpmB"
                     /locus_tag="PKJ17_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOD75895.1"
                     /translation="MARVCEICGKGKQIGYNVSHANNKTKREWRPNLQTVRIVKNGET
                     LKARLCTKCIKKGDFQKAV"
     gene            complement(858..1370)
                     /locus_tag="PKJ17_07710"
     CDS             complement(858..1370)
                     /locus_tag="PKJ17_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOD75896.1"
                     /translation="MKDPMTAASRTAFFRRPSFRLCFLMVLTLFPALPGLSAPDLPNV
                     SVVFFDAKDRELCRFEAEPAVTPEQQAQGLMFRSSLRRRTGMLFLHDRDEVQNYWMKN
                     TWIPLDLIFINGRYEVVHVHRGARPRDETNISSRYPARYILEVNAGEARECAITRGVR
                     VRFGGVPAGR"
     gene            complement(1474..>2426)
                     /locus_tag="PKJ17_07715"
     CDS             complement(1474..>2426)
                     /locus_tag="PKJ17_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD75897.1"
                     /translation="IPCDMLVLSMGVRPDTRLARGCGLAIGHLGGIKVNPLMMTSDPD
                     IFAAGDAVEVNDLVTGFPVLTALAGPANKQGRVAADNALGRRTVYRGTLGTSIVKVFG
                     LTVSSTGASEKTLRMREVPHLVSYTHSNSHAGFYPGAEMMTIKLAFSPGSGRILGCQI
                     VGGAGVDKRIDVMATAIRGSMTVFDLEDIDLAYAPPFSSARDPVNIAGFVASNILKGD
                     HEVVHAGDLKDIGENAVLLDLRDMEDLVRSSPIEGAVHVPLGRLRKKMEKLDREKLYI
                     TYCEVGTRSYVGHRVLAQRGFKSKNLSGGYKTYAATMKGN"
BASE COUNT          519 a          690 c          680 g          537 t
ORIGIN      
        1 ggttaccggt cacctgtatt cgtttgagaa tcgctcgatg ggggtatgaa cgtcgtatat
       61 cggtaggggg gtaaaggtga tggactttcc tatcacctgt aacccatgac ccatcacctg
      121 tcttcactac actgctttct gaaaatcacc tttcttgatg cacttcgtgc agagccttgc
      181 cttgagggtt tcgccgttct tcacgatgcg gacggtctgc aggttcggcc gccattcccg
      241 tttggtcttg ttgttcgcgt ggctcacgtt gtaaccgatt tgttttcctt ttccacagat
      301 ctcacagacc cttgccattt gtgacctcct gaccttgtcc gaacttcctt gagttccttt
      361 aaagacaaag attttacacg cttaaggcgt tctatgtcaa cacttttgtc ggcagggggc
      421 cgatcgggga acagaaaagg ggataggggc aggcccttca caggacgcat gggaatggac
      481 aggaccgata cgacatacag gacatatggg acgcatcaga tgcggttgtc cttcattcct
      541 caccttggag tttctcgcta tttttagtga atgatttcga taagatacgg ataggtgccg
      601 ggagataccg gtcgaacccc tgtaaatgat tggctaaaag cattccagcg tcggaccatt
      661 acgaaaaggc atcaaatcgg tgaattatct ctatgggcgt tgaaatgatt gctgttttca
      721 ccatgcacgg taaccgggca aaaggagcaa aaccttttag catcacgagg ttgaccgcgc
      781 atttccacaa cttatcaaca ggaaatccac aatcgttgtg gaaaagcagt ggcttcaccc
      841 ggtccggagg aaacctgtca gcgaccggcg gggacccctc cgaaacggac ccggacaccc
      901 cgcgtgatgg cacactccct cgcctcgccc gcgttcactt ccaggatata ccgggcggga
      961 tatcgtgagc ttatgttcgt ttcatcgcgc gggcgggcgc cccggtgaac atggacgacc
     1021 tcgtaccgtc cgttgatgaa gatgaggtcg agagggatcc aggtgttttt catccaatag
     1081 ttctggacct cgtcgcggtc atggaggaaa agcattcccg tgcgcctgcg cagagaggac
     1141 ctgaacatga gcccctgcgc ctgctgttcg ggggtcacgg ccggctccgc ttcgaaacgg
     1201 cagagttcac ggtctttagc atcgaagaac acgacgctga cgttgggaag gtccggagct
     1261 gaaagaccgg gtaaagcagg aaagagcgtc aagaccatca ggaaacagag acgaaaggag
     1321 ggccggcgga agaacgctgt gcgcgacgcg gcagtcatgg gatctttcat tccttatggt
     1381 gcgccggaca ggtggaaaag tcaagaagac aatcccaccg tggcgatcag gcgatggacc
     1441 gccctctccg gcctatgacg gccgggcaga ggatcaattt cctttcatgg tggcggcgta
     1501 cgttttgtat ccgccgctga ggttcttcga cttgaaacct ctctgcgcca gaacgcgatg
     1561 gcccacgtag gaccgcgttc caacctcgca gtaggtgatg taaagcttct ccctgtccag
     1621 tttctccatc ttctttctca gccgtccgag gggaacgtgc acggcccctt cgatcggaga
     1681 actcctcacc agatcctcca tgtccctcaa gtccagaagg accgcgttct ccccgatatc
     1741 cttcagatcc ccggcgtgga cgacctcgtg atcccccttc aatatgttgg aggcaacgaa
     1801 accggcgatg ttgacggggt ctctcgccga cgagaaagga ggcgcgtacg ccaggtcgat
     1861 atcttcgagg tcaaagaccg tcatggaacc gcgaatggcc gttgccatca catcgatgcg
     1921 cttgtccacc ccggctcccc ccactatctg gcatccaagg atccggccgc ttcccggtga
     1981 gaaggcaagt ttgatggtca tcatttcggc cccgggatag aatcccgcgt gcgagttcga
     2041 atgcgtgtaa ctgacgagat gcggcacctc gcgcatgcgc agcgtcttct cgctggcacc
     2101 cgtggacgac accgtgaggc cgaagacctt cacgatcgaa gtccccagtg tgcccctgta
     2161 gaccgttcgt cttcccagtg cattgtccgc cgcgaccctg ccctgcttgt tggccgggcc
     2221 ggcgagtgcg gtcagcacag ggaagccggt gacgagatcg ttgacctcaa cggcgtcccc
     2281 ggcggcgaaa atatccggat cggaggtcat catcaacggg ttcactttta tgcctccaag
     2341 atggccgatc gcgagaccgc agccccgggc aagccttgtg tccggccgga ctcccatcga
     2401 aagaacgagc atgtcgcagg ggatct
//
LOCUS       DAOPOA010000353         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_47309_length_2426_cov_3.794601, whole genome shotgun sequence.
ACCESSION   DAOPOA010000353 DAOPOA010000000
VERSION     DAOPOA010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47309_length_2426_cov_3.794601"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="PKJ17_07720"
     CDS             <1..1353
                     /locus_tag="PKJ17_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75898.1"
                     /translation="HSSRESFPEKPEGPDEGQSGKGPAYFERLSNTVRELNTTLMRNN
                     INHGKLIEILRSDGFDHLRLAEFLRTGRLDNRCVVDVSEWLLLRTFLYDLINAPKVFK
                     NDYKMLLRVLTKVNKSLLVYIWTLGLEKDPRFEMVSAYISKVMHDIFVFDRVKIYEIV
                     SDPAECLEAEAVTGYLLNWAGFTPEVRRSIIELLSGVPDHVVRQLRVKTTDDVDRMVR
                     MMGTRASGLIGFYLHQVYRWGSAEDRLTARIRSHFPELLRQAQKSMRPGEMIRGGAML
                     LLLLAEITSDERDGFAGNIVITEDERRTVRDLIKRCVDWDQRHFVMMVAGTAAGYMGS
                     IDGWVLQQFDKLLARYDGGDRVMLRSVVQFDVDNLIERALLTAGDGEENSLLNKVQLL
                     RFGVDPVVSDEANSYLSACARRLGIHAPEDNAKKKDIRDFLLALSRLMKEETRRSVAY
                     "
     gene            complement(1431..>2426)
                     /locus_tag="PKJ17_07725"
     CDS             complement(1431..>2426)
                     /locus_tag="PKJ17_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75899.1"
                     /translation="DMDKEKAREKLTLAERSIARAKELTQQLLTFSRGGAPITKVASI
                     TDHLRETAQFTLTGTGVTCRFDIADDLLPVAIDEGQINQAIGHIVINAHQAMPQGGTI
                     LIGARNVTFGNADINLPAGRYVHISITDNGIGIPEEHLDRVFDPYFTTKQKGSGLGLA
                     ICYSIIKNHHGGITVESTLGVGSTFHIYLPVHDAPSLGERRNGEDMTKVIGGNVLVMD
                     DEEAILEIVGDILAYHGFTVDFARDGEEALCLYQERRYDAVILDLTVPGGMGGREAMK
                     ELLRIDPAVRAIVSSGYSNDPIMSDYTQYGFKNVIAKPYDLEELGEVLRSVIGGN"
BASE COUNT          465 a          695 c          751 g          515 t
ORIGIN      
        1 cattcctcac gggaaagttt cccggagaaa ccggaaggtc ccgacgaagg acagagcggg
       61 aaggggcccg cttacttcga gcggctgtcg aacacggtcc gggagctcaa cacgaccctg
      121 atgcggaaca acatcaatca cggaaagctg atcgagatcc tgcgcagcga cggcttcgac
      181 caccttcggc tggccgaatt cctgcgcacg ggccgtctcg acaacagatg cgtcgtggat
      241 gtcagcgaat ggctgctgtt gaggacgttc ctgtacgacc tcatcaacgc gccgaaggtc
      301 ttcaagaacg actacaagat gctgctgagg gtcctcacga aagtgaacaa gagcctcctt
      361 gtctatatct ggacgctggg tctcgagaag gacccgcgtt tcgagatggt gagcgcctac
      421 atatcgaagg tgatgcacga tatctttgtc ttcgatcgcg tgaagatata cgagatcgtc
      481 tcggacccgg cggagtgtct cgaggccgag gccgttacgg ggtatcttct caactgggca
      541 gggttcacac ccgaggtgcg gcgatcgatc atcgagttgc tgtcgggggt tccggaccat
      601 gtggtccggc aattgagggt gaagacgacg gacgatgtgg accggatggt ccgcatgatg
      661 ggaacgcggg ccagcggcct cataggtttc tacctgcatc aggtctaccg ctggggaagc
      721 gccgaggacc ggctgacggc aaggatccgc tcccatttcc cggaacttct gaggcaggcg
      781 cagaagtcca tgcgtccggg ggagatgatc aggggagggg ccatgctcct tctcctcctg
      841 gcggagataa cctccgacga gcgcgacggg tttgccggca acattgtgat aaccgaagac
      901 gaaagacgaa ctgtccgcga cctcatcaag cggtgcgtcg actgggacca gcggcatttt
      961 gtcatgatgg tcgcgggtac tgctgccggc tacatgggga gcatcgacgg gtgggtcctg
     1021 cagcagttcg acaaactgct ggcccgttat gacggcgggg accgcgtgat gctgcgcagc
     1081 gtcgtgcagt tcgatgtcga caacctcatc gagcgcgccc tgctgacagc cggagacggc
     1141 gaggagaact cattgctcaa caaggtgcaa ctcctgcgtt tcggggtcga ccccgtcgtc
     1201 agcgacgagg ccaacagcta cctctcggca tgtgcccggc gcctcgggat tcacgcgccc
     1261 gaggacaacg caaagaagaa agacatccgg gacttccttc tcgccctgtc gcgcctgatg
     1321 aaagaagaga cccgtcgctc ggttgcatat tagaacggca ggctcgaccc ccctcatatg
     1381 tctctaaagg tgtttttcca acgttcaagc cattcaagcc gtctttcccg ctagtttccc
     1441 cctatcaccg acctgaggac ctcgccgagc tcctcgaggt cgtagggttt cgcgatgacg
     1501 ttcttgaaac cgtattgggt gtaatcggac atgatgggat cgttggagta accgctcgat
     1561 acgatggcac gtaccgcggg gtctatcctg aggagttctt tcatcgcctc ccgtcccccc
     1621 attccgccgg gcacggtaag gtcgagtatc accgcgtcgt accgtctttc ctggtaaagg
     1681 cagagcgctt cctcgccgtc ccgggcaaaa tccacggtga agccgtgata ggccaggata
     1741 tctccgacta tctccagtat cgcttcctcg tcgtccatga cgaggacgtt tcccccgatc
     1801 accttggtca tgtcctctcc gtttctcctt tcccccaaag atggtgcatc gtgcacgggc
     1861 aaatatatgt gaaaggtgct tcccacaccc agtgtcgact cgacggttat gcccccgtga
     1921 tggttcttga tgatggagta acagatggcc agtccgaggc cgctgccctt ctgcttggtg
     1981 gtgaagtagg gatcgaagac gcggtcgaga tgctcctcgg ggatgccgat accgttgtcc
     2041 gtgatgctga tatggacgta tctgccggcc gggaggttga tgtcggcatt cccgaaggtc
     2101 acgttccggg ctccgatgag tatcgttccg ccctggggca tcgcctggtg ggcattgatg
     2161 acgatgtgcc cgatggcctg gtttatctgg ccttcgtcga tggcgacggg caggaggtcg
     2221 tcggcgatgt cgaacctgca ggtaacgccg gtgcccgtca gggtgaattg ggccgtttcc
     2281 ctcaggtgat cggttatcga cgcgaccttc gtgatgggcg cgcccccgcg ggagaaggtg
     2341 aggagctgct gggtgagttc cttcgcccgc gcgatggagc gctcggcaag ggtgagcttc
     2401 tccctcgctt tttccttgtc catgtc
//
LOCUS       DAOPOA010000354         2422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_47430_length_2422_cov_2.851711, whole genome shotgun sequence.
ACCESSION   DAOPOA010000354 DAOPOA010000000
VERSION     DAOPOA010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47430_length_2422_cov_2.851711"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="PKJ17_07730"
     CDS             <1..1577
                     /locus_tag="PKJ17_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOD75900.1"
                     /translation="SVSKRKIMKIRLTALIILAGILFFFNLGTTSLWDPDEPRQAIMA
                     REMMDRGDYIRPYLNGIPYLEKPPFYSWMIIAAAKACGSVDEFASRAPSALAALLTVV
                     MTFLLGCRLGDERAGFFSGLVLATNYQFLSNARESVMDMTFAFFIGLAIYLAFVSLEK
                     GKRLNFALSFIPGAFAILTKGPAGLVIPVGVVFVYLVFKKSLRRYIVPLVLGSVLATA
                     IASVWFLAAGESYIREFILHQNVTRYTNAFDHLESPWYYFHKLFFNFMPWSLFLPFAL
                     FGAWKRRYVLPLLWFVLIFLFFELSQSKRAIYLLSAYPALALLTGLYIRDAWDGLVER
                     GWTNTVLKALAVLLALIPVGAVIAMYVLPEEDVAVFRDGPLSLYVYLAVLFLAACGFL
                     VMLFKRNRHFALAFFLFFLVFTGFFYGAYYMPVVDRTSKSLTLITDALGDDGRTKRIY
                     TYGFNSAGIIFYVGKPVKALSDIKEIKEDESDILLIVEEKPTMHLRETLEKSFVPVKR
                     VKYEKEHYIFYVRKNG"
     gene            1570..>2422
                     /locus_tag="PKJ17_07735"
     CDS             1570..>2422
                     /locus_tag="PKJ17_07735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD75901.1"
                     /translation="MDEKPYVSVLVPVLNEEESLPELNERLRDALAAIHRPYEIIYIN
                     DGSTDRTEGILETFHREDRRIKVIEFNRNYGQHMALFAGFDFARGEIVVTIDADLQNP
                     PEEIPRLVAKAEEGYEVVATYRKQRKDSLFRKLPSYIVNRITAKLVGVRLKDYGCMLR
                     AYRRNIVDYMNMCPESSSFIPALANTFAKKITEIEVGHEERKKGTSKYSPFKLFRLNF
                     DLMTNFSLLPIQFISMLGVAIAFLGLAFAIFLMVRRLLIGPEGEGTFTLFGVLFFFIG
                     IQIFALGV"
BASE COUNT          484 a          716 c          638 g          584 t
ORIGIN      
        1 gtagtgtttc gaagcgaaag ataatgaaaa tacgacttac agcgcttatt attctcgcgg
       61 gcatcctctt ttttttcaat ctcgggacca cctccctgtg ggaccccgat gaaccgcgtc
      121 aggccatcat ggcacgggag atgatggaca ggggggacta tatcaggccc tacctcaacg
      181 gtatcccgta cctggagaag ccgccattct attcatggat gatcatcgcc gcggcgaagg
      241 catgcggctc ggtcgatgaa tttgcctccc gggcgccctc cgcgctggcg gcgctcctta
      301 ccgtggtgat gacctttctc ctggggtgca ggctcggcga cgaacgcgcg ggtttcttct
      361 cggggcttgt tctcgcgacc aattaccagt tcctctccaa cgccagggaa tcagtgatgg
      421 acatgacctt tgcctttttc atcggtcttg ccatctacct ggcctttgtc tcactggaga
      481 agggcaagcg tctcaacttt gccctttcct tcatccccgg cgctttcgcg atactcacga
      541 agggaccggc aggacttgtc atacccgtcg gggtcgtgtt cgtctatctc gtgttcaaga
      601 agagcctccg ccgctacata gttcccctgg tgctcggctc tgtccttgca accgccatcg
      661 cctccgtgtg gttccttgcc gccggcgaga gctacatcag ggagttcatc cttcatcaga
      721 acgtgacccg ctacacgaat gccttcgacc acctggaatc gccctggtat tactttcata
      781 aacttttctt caatttcatg ccctggagcc tcttccttcc ctttgccctt ttcggggcct
      841 ggaaacggcg ctatgttctt cccctcctct ggttcgtgct catcttcctt ttcttcgagc
      901 tgtcccagag caagagggcg atctatcttc tctccgccta tccggcactc gcgcttctca
      961 ccgggcttta catcagggac gcctgggacg gcctcgtcga aaggggttgg acgaacaccg
     1021 tcctcaaagc actggcggtg ctgctcgctc tcatacccgt cggtgccgtc atcgccatgt
     1081 acgtgctgcc cgaggaggat gtggcggtct tcagggacgg gccgctgtcc ctgtatgtct
     1141 atcttgccgt gctgtttctc gcggcatgcg gttttctcgt catgcttttc aagaggaaca
     1201 ggcacttcgc cctggccttt ttcctcttct ttcttgtttt caccggtttt ttctacggcg
     1261 cctactacat gcccgtcgtc gacaggacgt cgaagtccct gaccctcatc acggacgccc
     1321 tcggcgatga cggacgaacg aagaggatat acacctatgg tttcaactcc gccggcatca
     1381 tattctacgt ggggaaaccc gtcaaagccc tgagcgatat taaggagata aaagaagacg
     1441 aaagtgatat actactcatt gtcgaagaga aacccacaat gcatctcagg gagactctgg
     1501 agaagtcctt cgtgcccgtg aaaagggtga agtacgagaa ggagcactat atcttctatg
     1561 tgaggaaaaa tggatgagaa gccttatgtg tccgtgcttg tccccgttct gaacgaagag
     1621 gagtcgctcc cggaactgaa cgagcggctc cgcgacgctc tggcggccat tcacagaccc
     1681 tacgagatca tctatataaa cgacgggagc acggaccgaa cggaggggat cctcgagacc
     1741 ttccatcgcg aggacagacg catcaaggtc atagagttca acaggaatta tggtcagcac
     1801 atggcccttt tcgccggttt cgatttcgcc cggggcgaga tcgtggtgac catcgatgct
     1861 gatctgcaga acccgcccga agagatcccg aggctcgtgg ccaaggcgga agagggttac
     1921 gaggtcgttg ccacgtaccg gaagcagagg aaggattcgc tgttccgcaa gctgccttcc
     1981 tacatagtca acaggatcac agcgaagctc gtcggcgtcc gcctcaagga ctacggctgc
     2041 atgctcaggg cgtaccggcg taacatcgtc gactacatga acatgtgtcc cgaatcgtcg
     2101 agcttcatcc cggcgcttgc caataccttt gcgaagaaga taacggaaat agaggtcggc
     2161 cacgaggaaa ggaagaaggg gacgtccaag tacagcccct tcaagctctt caggctcaat
     2221 ttcgacctca tgacgaactt ttcgctcctg cccatacagt tcatcagcat gctcggtgtg
     2281 gccatcgcct ttctcggtct tgccttcgcg atcttcctca tggtgcgacg gctcctcata
     2341 ggccctgaag gggaggggac cttcaccctc ttcggcgtgc tcttcttctt catcggcata
     2401 cagatcttcg ccctgggggt ca
//
LOCUS       DAOPOA010000355         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_47477_length_2420_cov_3.514165, whole genome shotgun sequence.
ACCESSION   DAOPOA010000355 DAOPOA010000000
VERSION     DAOPOA010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47477_length_2420_cov_3.514165"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKJ17_07740"
     CDS             complement(<1..264)
                     /locus_tag="PKJ17_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75902.1"
                     /translation="MGHIRPEWIHGYGDADAREQTRVTKMTNNQIPITNEKDNDQFTN
                     NQLNEAKGGLPVSFLSWLLVIVCFSGLTSDVNSRHQYISHAATF"
     gene            419..1624
                     /locus_tag="PKJ17_07745"
     CDS             419..1624
                     /locus_tag="PKJ17_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOD75903.1"
                     /translation="MPQFQWEGRTSSGETRRGVSKASSGDALRAALRKEGVILTSMQE
                     TKEAGKQKYNPQKKIKNIEILLFTRQLSTMITSGLPLVQSLEILGNQIEDVDFRGIVK
                     EIKEKIEGGARFADALRDYPKCFDELFINLVVAGEEGGLLDNVLQRLAIYIEKTEKLK
                     KKVKSAMIYPVAIIVVAFVVVVVLLLFVIPVFEKMFKEMGAELPVPTQIVINLSQMMQ
                     SYWYIIIGGVVGLVLALKYYYKTEGGHRNIDRIILKLPLFGLLTIKASVARVTRTLAT
                     LLMSGVAILESMIIVAKVAGNKIVEEALLVARSRISEGRTMAEPLEQAGIFPPMVVQM
                     VQVGESTGALDSMLNKIADFYEEDVDNMVTNLTAMMEPMIMVFLGVVLGGLIIAMYLP
                     IFKLGQAVG"
     gene            complement(1648..1836)
                     /locus_tag="PKJ17_07750"
     CDS             complement(1648..1836)
                     /locus_tag="PKJ17_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75904.1"
                     /translation="MREDRLFKDKDILFLSRRDSGTRRPPDLGGLQDPAAIFSLFYPL
                     FAVSADSREELQRRTGVR"
     gene            complement(2114..>2420)
                     /locus_tag="PKJ17_07755"
     CDS             complement(2114..>2420)
                     /locus_tag="PKJ17_07755"
                     /inference="COORDINATES: protein motif:HMM:NF018651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75905.1"
                     /translation="VDSVPINILNAIPGTPLENLPALSPMEILLTVALYRFILPDKDI
                     KLCGGKEKNLRQLLPLAMVAGCNSLLTGNYLTTLGRDTSADLEMIHDLGMVAKFDEA"
BASE COUNT          547 a          640 c          656 g          577 t
ORIGIN      
        1 gaaagtcgcg gcgtgagaaa tatattggtg tctactattg acatcggagg tcagaccaga
       61 gaaacagaca ataaccaata accaagaaag aaacgataca ggcagtccgc ctttcgcttc
      121 gttcaactgg ttattggtga attggtcatt atctttttca ttggttattg gaatttgatt
      181 attggtcatt tttgttaccc tggtttgttc ccgagcgtca gcatctccat aaccatggat
      241 ccattctgga cggatgtgac ccatgacccg ttttctctgt gacgtttgtc acatttttgt
      301 ttaattataa ccgtagttta tagtgtaaga atcatattct ggaactcgtt tctccattaa
      361 ccacctgttt tgataatttt ctctttgcaa acgggattta ttcatgtatt attgagtcat
      421 gccgcagttt caatgggaag gaaggacttc gtcgggtgaa acgcgcaggg gcgtttcgaa
      481 ggcgtcatcc ggcgacgccc tgcgggcggc gctgagaaag gaaggcgtca tcctcacctc
      541 catgcaggaa acgaaggagg cggggaagca gaagtacaat ccccagaaga agataaagaa
      601 catcgagata ctgctcttca cgcggcagct ctccacgatg atcacatccg ggctccccct
      661 tgtccagtcc ctggagatcc tcgggaacca gatagaggat gtcgatttca ggggtatcgt
      721 caaggagatc aaggagaaga tagaaggggg ggcgcgtttc gccgacgccc tgagggacta
      781 tccgaagtgt tttgacgaac ttttcatcaa cctcgtcgtg gcgggcgaag agggtggtct
      841 cctggacaac gttctgcaga ggcttgccat ctacatcgag aagacggaga agcttaagaa
      901 gaaggtcaaa tcggccatga tatacccagt cgcgatcata gtcgtggcct ttgtcgtcgt
      961 cgttgtcctc cttctcttcg tcatacccgt tttcgagaag atgttcaagg agatgggagc
     1021 ggaactgccg gtgccgacgc agatcgtcat caacctgagc cagatgatgc aatcctactg
     1081 gtatattatc atcgggggtg ttgtcggtct cgtcctggcc ctgaaatact actacaaaac
     1141 ggaaggcggt caccgcaaca tcgaccgcat catcctcaaa ctccctcttt tcggtctcct
     1201 caccataaag gcctccgtgg caagggtgac gcggaccctg gcgacgctcc tcatgagcgg
     1261 tgtcgcgatc ctcgagagca tgatcatagt cgcgaaggtg gcgggcaaca agatcgtcga
     1321 ggaggccctt ctggtggccc gctcccgcat cagcgagggc cgcacgatgg ccgagcccct
     1381 tgaacaggcg ggcatctttc cgcccatggt ggtccaaatg gtccaggtcg gtgaatcgac
     1441 gggcgccctc gacagcatgc tcaacaagat cgccgatttc tacgaagagg acgtggacaa
     1501 catggtcacc aaccttaccg ccatgatgga gccgatgatc atggtcttcc tcggcgttgt
     1561 cctcggcggc ctcatcatcg cgatgtatct ccccatcttc aaactcggcc aggcggtcgg
     1621 gtaaacagcc gaagaaagag aaagactcta tctcacgccc gttcgtcgct gaagctcctc
     1681 tctcgagtcc gcagagaccg caaagagagg ataaaagaga gaaaagattg ccgccggatc
     1741 ttggagccca ccaagatccg gcggccttct cgtcccgctg tcacggcggg agagaaagag
     1801 aatatcctta tccttaaaca acctgtcttc cctcatcccc ccctgtagtc ataggtccta
     1861 tgggtcctat aggtcctatg ctctctcctc caacccctat accacaaccc cacaccttct
     1921 tatcctctcc cccattactc ctgcctgaaa ccaaaagttt ctttttccct cccgcgaagc
     1981 gggatgcaaa gacggctata tcccggctgg tgccgggata tagccgaatc ttttctctgt
     2041 gtgctctcct cacaacctct gtgtgctcga gtgaggagcg aacgcgacga acgggcgtga
     2101 tgcttttctg ttttcaggct tcgtcgaact ttgcgaccat ccccaggtcg tggatcattt
     2161 ccaggtcggc ggaggtgtcc cttccgaggg ttgtgaggta gttgccggtg agcagcgagt
     2221 tgcagccagc caccatggca agcgggagaa gttgtcttaa attcttttcc ttgccgccgc
     2281 agagttttat gtccttgtcg gggaggatga agcggtaaag ggcgacggtg aggaggatct
     2341 ccatcgggct cagcgcgggg aggttctcga ggggcgtgcc ggggatggcg ttgaggatgt
     2401 tgatgggaac ggaatccacg
//
LOCUS       DAOPOA010000356         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_47482_length_2420_cov_3.332347, whole genome shotgun sequence.
ACCESSION   DAOPOA010000356 DAOPOA010000000
VERSION     DAOPOA010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47482_length_2420_cov_3.332347"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKJ17_07760"
     CDS             complement(<1..275)
                     /locus_tag="PKJ17_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75906.1"
                     /translation="MEREQRIAVASEIMELFAELTGLVPGGVSPRRYLWTDAYGVCNF
                     LGLYRETGDVAYRDLALRLVDDVHHTLGRHRKGDARQGWISGLDEDGG"
     gene            520..984
                     /locus_tag="PKJ17_07765"
     CDS             520..984
                     /locus_tag="PKJ17_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75907.1"
                     /translation="MLRARPGSWFSWKFNILDENDQIIAGIDIRWMREAGELHLGGRS
                     YRLFREGLFGGAFILEEDGMELARAEKPSSLFRSFTVTAKGRAYRLHAASPVGRTFIL
                     SENDRTIGVIRPENAFTRKAVIDLPEEIALPVKIFMAWLVFVLWKRDEESSS"
     gene            1185..>2420
                     /locus_tag="PKJ17_07770"
     CDS             1185..>2420
                     /locus_tag="PKJ17_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD75908.1"
                     /translation="MTRARRIVVIGGSAAGPKAAARARRMDETADITIIQKDPDLSMA
                     SCGYPYYVGGFFDDRNMLLSTPTGVTRDPRFYINAKGIVAKTETEALGIDRHARTVTL
                     RDLRSDEVTVVEYDKLIIATGSIPRLPPVPGTGLSGITTLQSMRDADYLRRVRDEGKI
                     RKAVIIGGGLIGIETSEALELAGMEITVVELLPQLLTFLDWEIAKLVENHVKSKNANV
                     ITGNGLAAFLGEDGKLTAVKLSNGTELPCELAVVAIGVRPNVNLAAEAGLEIGQTGGI
                     TVDKYMRTSDPDIYAVGDCVEVTNLITGKKIADEPTEADVLDMAQAYADAAADAKRLG
                     FDCIELHGAHAYLIDEFFWPVTNQRTDRFGGAKLEQRAEFAAEALRRCRAAIGDLPMI
                     IRVSQWKSVDYNAKLATTPQ"
BASE COUNT          524 a          772 c          688 g          436 t
ORIGIN      
        1 ccgccatcct cgtccagccc gctgatccaa ccctgtctcg cgtcgccctt gcggtgcctg
       61 ccgagtgtat gatgcacatc gtccaccagc cgcagcgcaa ggtccctgta tgcgacgtcc
      121 ccggtctccc gatagagtcc gagaaagttg cagaccccgt acgcatccgt ccacaggtac
      181 cgcctgggag aaactcctcc gggaacaagc cccgtaagct cggcaaacaa ctccatgatc
      241 tctgacgcga cggctattct ctgctccctt tccatgatcc cttcctttgt gtactgtgaa
      301 catcacccct gcaacaatta accaactata gctccacgcc cgcaaaacgt caaataccga
      361 tccccgacac cgggcgtcat catgtctttc acgctccctc ttgccttttc cttttccacc
      421 atatataaaa gtatgggtgc gcaaccgaat cctcccggtc cgcgatcccg gacgcagcgc
      481 aaaggcgagg aaataccctg ctgaggatgc cgccatctca tgctcagagc ccggcccggg
      541 agttggttct cctggaaatt caacattctc gacgaaaacg accagataat cgccggaatc
      601 gacatccggt ggatgcgtga ggccggcgag ttgcaccttg gcggaaggag ctacagactc
      661 tttcgtgaag gtcttttcgg aggggctttc atccttgaag aagacgggat ggaactggcg
      721 cgggccgaaa agccgagttc cctgttccgg tccttcacgg tcaccgcgaa gggcagggcg
      781 tacaggctgc atgccgcgtc cccggtggga cgcacgttca tcctgagcga gaacgatagg
      841 acgatagggg tcatcaggcc cgaaaacgcc ttcacgagga aggcggtcat cgatctgccg
      901 gaggagatcg ccctgccggt gaagatcttc atggcgtggc tcgtgttcgt tctctggaag
      961 agggacgaag aatcgtcgtc ctgacccgcc gaaggccgcg gccggcgcgg tcgctgcccc
     1021 gtttcgacgg ggtcccctcc ccgtcgccct tgattttccg gttatggata tatagtatca
     1081 actatgatat agctcaggat accacggagt cttcagagtc tttgggtacc cgccgcaaca
     1141 tcgggatccg ctgtgcgtca tagccaccaa aaaggagaga cttcatgacc agagcaagaa
     1201 gaatagtcgt tatcggcggg tcggcggccg gcccgaaggc tgcggcccgg gcccgaagga
     1261 tggacgagac agccgatatc accataatcc agaaggaccc cgacctttcc atggcctcat
     1321 gcggatatcc ttactatgtc ggcggcttct tcgatgacag gaatatgctg ctcagcacgc
     1381 ccacgggtgt caccagggac cccaggttct acatcaacgc caagggcata gtcgcgaaga
     1441 ccgagaccga ggccctcggc attgaccgtc atgccaggac agtgacattg agggacctca
     1501 ggtccgatga ggtgacggtc gtcgaatacg acaagctcat catcgcgacg ggttcgatac
     1561 ccaggcttcc ccccgtcccc ggcacgggac tttcgggcat aaccaccctg caatcgatgc
     1621 gggacgcgga ttacctcaga agggtgcggg acgagggaaa gatcaggaag gccgttatca
     1681 tcggcggggg cctgatcggc atcgaaacga gcgaggccct cgaacttgcc gggatggaga
     1741 tcaccgtggt ggaactcctg ccgcagctcc tcaccttcct ggactgggag atcgcgaagc
     1801 tcgtcgagaa ccacgtgaaa agcaagaacg ccaacgtgat aaccgggaac ggactcgcgg
     1861 ccttcctggg cgaggatggc aagctcaccg ccgtcaagct cagcaacggg acggagcttc
     1921 cctgcgaact cgccgtcgtg gccatagggg tgagacccaa cgtgaatctt gccgccgagg
     1981 caggcctcga gatcggacag accggaggta tcacggtcga caagtacatg cggacctccg
     2041 atcccgatat ctatgccgtc ggcgactgcg tggaggtcac gaacctcatc accggcaaga
     2101 agatcgccga cgagcccacc gaagccgacg tcctcgacat ggcacaggct tacgcagacg
     2161 ccgccgccga cgctaaacgc ctcggcttcg actgcatcga actccacggc gcccacgcct
     2221 atctgatcga cgaattcttc tggcccgtca ccaaccagcg cacagaccgc ttcggtggcg
     2281 caaagctgga acagcgcgcc gagttcgcag ccgaagcgct ccgtcgttgc cgcgccgcca
     2341 tcggcgacct gccgatgatc atccgcgtct cgcaatggaa gtcggtcgac tacaacgcta
     2401 agctcgcgac cacgccgcag
//
LOCUS       DAOPOA010000357         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_47673_length_2413_cov_3.360475, whole genome shotgun sequence.
ACCESSION   DAOPOA010000357 DAOPOA010000000
VERSION     DAOPOA010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47673_length_2413_cov_3.360475"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PKJ17_07775"
     CDS             <1..768
                     /locus_tag="PKJ17_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75909.1"
                     /translation="FYPGVGYRHIMIWKGGASRLSTTPPHDITGKQIAGYLPDGDGAA
                     RVRSLMDHSREVLASHEVNRKRVDAGKLPANSIWLWGQGKKAALPSFREKFGLTGATV
                     CAVDLVRGISSLVGFTTPVVEGATGYLDTDYGAKARAALKLLEDHDIVYVHVEAPDEA
                     SHSGSIPEKIKAIERIDRDVLGVLLKETDRDTRFLIVTDHATPIAMRTHYATPVPFAV
                     YDRSDPASEEGGDYDENAGGAVISGEEMIRILIGGRQ"
     gene            765..>2413
                     /locus_tag="PKJ17_07780"
     CDS             765..>2413
                     /locus_tag="PKJ17_07780"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOD75910.1"
                     /translation="MRQKFIFVTGGVVSSLGKGIAAASIGALLESRGLNITIKKLDPY
                     INVDPGTMNPFQHGEVFVTEDGAETDLDLGHYERFTTAAFSKKCNFTTGQVYDTVISR
                     ERKGEYLGGTVQVIPHITDEIKRRILDVDEGIDVVIVEIGGTVGDIEGLPFLEAIRQL
                     RNDLGKENSLFVHLTLVPFIKTADEMKTKPTQHSVNELRRIGIQPDILLCRTERALSQ
                     GLKDKISLFCNVEKDAVIAAKDAEVIYEVLIAFHNEGLDEKITKLLNIWAKKPDLSKW
                     EAIGDTIKHPKKEVDIALVGKYVKLKDSYKSLNEALVHGGIANDCRVNIRYVDSEDIE
                     KGITADLFNVDGVLVPGGFGDRGIEGKINAIEHAREKKIPFLGLCLGMQLAVVEMGRN
                     LADCKDANSCEFNEKTPYPLIYLIEEFTDRENKVQKRDQYSDKGGTMRLGAYPCVLKA
                     GTLAHKAYGQDVIHERHRHRYEFNMKYRERLEKSGMIVSGISPDGTLVEIVEMKGHPW
                     FLGCQFHPEFKSKPFDPHPLFRDFIKASLKRRQERLKCKKK"
BASE COUNT          558 a          751 c          721 g          383 t
ORIGIN      
        1 ttctaccccg gggtcgggta ccgccatatc atgatatgga aaggcggtgc ctcaaggctc
       61 tcgacgaccc ctccccatga tatcacggga aagcagatcg ccgggtacct ccccgacggt
      121 gacggcgcgg cccgggtccg ctccctcatg gaccactccc gcgaggtgct cgcgtcccat
      181 gaggtcaaca ggaaacgtgt cgacgccggc aagctcccgg cgaacagcat ttggctctgg
      241 ggccagggca agaaggcggc cctgccgtca ttcagggaaa agttcggcct gacgggtgcg
      301 acggtctgtg ccgtcgacct cgtgcggggg atatcgagcc tcgtggggtt cacgaccccc
      361 gtcgtcgagg gggccacggg atacctcgac acggattacg gggcgaaggc ccgggcggcg
      421 ctcaagctcc ttgaagacca cgacatcgtc tatgtccacg tggaggcgcc cgacgaggcg
      481 tcccacagcg gcagcatccc tgaaaagata aaggccatcg agcgcatcga cagggacgtg
      541 ctcggcgtcc ttctcaaaga aacggacagg gacacgcggt tcctcatcgt caccgaccat
      601 gccacgccga tcgcgatgag gacccactac gccaccccgg tgcccttcgc cgtttacgac
      661 aggagcgatc ccgcctccga agagggcggt gattacgacg agaacgcggg cggggcggtg
      721 ataagcggtg aagagatgat acgtatactg atcgggggaa gacagtgaga caaaaattca
      781 tcttcgtgac gggcggcgtc gtgtcgtccc tggggaaggg catagcggcc gcgtccatcg
      841 gcgcgctcct cgagtcccgg gggctcaata tcaccataaa gaaactggat ccctacatca
      901 atgtcgatcc gggcacgatg aaccccttcc agcacggtga ggtcttcgtg accgaggacg
      961 gcgcggagac ggacctcgac ctcgggcatt acgagcgctt caccaccgcg gccttctcga
     1021 agaaatgcaa tttcacgacg ggccaggtct acgacacggt catctcccgt gaacgaaagg
     1081 gagagtatct cggcgggacc gtccaggtca tcccccacat cacggacgag atcaagcgca
     1141 ggatcctcga cgtggacgaa ggcatcgacg tcgtcatcgt cgagatcggc ggcaccgtgg
     1201 gcgacatcga aggcctgccc ttcctggagg cgatacgtca gcttcgcaac gacctgggga
     1261 aggagaactc cctcttcgtg cacctgacat tggttccctt catcaagacc gccgatgaaa
     1321 tgaagacgaa gccgacacag cacagcgtga acgaactcag aaggatcggt atccagcccg
     1381 acatcctcct gtgcaggacg gagcgggccc tgtcgcaggg cctcaaggac aagatctccc
     1441 tcttctgcaa cgtcgaaaag gacgccgtca tcgccgccaa ggacgccgag gtcatctacg
     1501 aggtcctcat agccttccac aacgaggggc tcgacgagaa gataacgaaa ctcctcaaca
     1561 tctgggcgaa gaagccggac ctttccaagt gggaggccat cggcgacacc ataaagcacc
     1621 cgaagaaaga ggtggacatc gccctcgtcg ggaagtacgt caaactcaag gactcctaca
     1681 agagcctcaa tgaggccctc gtccacggag gcatcgcgaa cgactgcagg gtgaacatcc
     1741 gctacgtcga ctccgaggac atcgaaaagg gcataacggc cgacctcttc aacgtcgatg
     1801 gcgtcctcgt ccccggcggc ttcggggacc gcggcatcga gggaaagatc aacgccatcg
     1861 agcacgcgcg ggaaaagaag atccccttcc tcggtctgtg cctcggcatg cagctcgccg
     1921 tcgtggagat ggggcgcaac ctcgccgact gcaaggacgc gaacagctgc gagttcaacg
     1981 aaaagacgcc ctatcccctc atctatctca tcgaggagtt cacggacagg gagaacaagg
     2041 tgcagaaacg agaccagtac tccgacaagg gcggcaccat gcgcctcggc gcatacccct
     2101 gcgtcctcaa ggccggcacc ctcgcgcaca aggcctacgg gcaggacgtc atccacgaac
     2161 ggcaccggca ccgttacgag ttcaacatga aataccgcga acgcctcgag aagagcggca
     2221 tgatcgtgtc gggcatctcc cctgacggca cgcttgtcga gatcgtggag atgaagggtc
     2281 atccctggtt cctgggatgc cagttccatc ccgagttcaa gtcgaagccc ttcgaccccc
     2341 atccgttgtt ccgggatttt atcaaggcct cgctgaaacg aaggcaggaa agactgaagt
     2401 gcaaaaagaa ata
//
LOCUS       DAOPOA010000358         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_47677_length_2413_cov_3.002120, whole genome shotgun sequence.
ACCESSION   DAOPOA010000358 DAOPOA010000000
VERSION     DAOPOA010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47677_length_2413_cov_3.002120"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /gene="lpxC"
                     /locus_tag="PKJ17_07785"
     CDS             <1..1175
                     /gene="lpxC"
                     /locus_tag="PKJ17_07785"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOD75911.1"
                     /translation="EGFSVLKATDGKEGLAIFEREAPDVVLLDVWMPELDGIQVLKEI
                     RARKDDAKVVVISGHGTISTAVEAVKMGAYDFLEKPLSIDKVLEVVSRSIGAGLKHDP
                     SVADFRLEVAPGITREKQKTIGKSTVVYGVGLHSGVKTGMILLPMPENTGIVFETIPD
                     GERIPAYIDYVFSVGYASSVKGKNSVVRTIEHLLSACHMYGITNLLVKVSEEIPIFDG
                     SAIEICRKIDEAEIVEQTDGIEPVVITEKVELPNLQNGKYLAAEPSERFEIDYLLEQK
                     RPIGTQHYRFVAGRDDYVANIAPARTFGFLKDFEQLAKMGLGTGGRINNFIILGEDEV
                     INTKLRFEDEFVRHKILDLIGDIYLLNRPITGKITARQTGHVENIALVKELKKRFL"
     gene            complement(1354..2043)
                     /locus_tag="PKJ17_07790"
     CDS             complement(1354..2043)
                     /locus_tag="PKJ17_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOD75912.1"
                     /translation="MKDRALRRTRNLSSPVLFGVLAVFLLIPFFAGGDATSAAKGETG
                     SPFDSIKKTYAGITALEASFHQKIFVSGIKKIRDFDGNFYFKRHKGFLWKYTKPKGKT
                     FLYDGHYMWQDEEGKTFVLKSKVSREKTGGTFFDLVEDIARIDNLFTVKQHSMLAGMQ
                     YFELLPKKDSTVNIAKIWIDKNNLLRKIEILEFTGNVNTIEFTDIKVNAPVSDSKFTY
                     KSDGIKDVVER"
     gene            complement(2064..>2413)
                     /locus_tag="PKJ17_07795"
     CDS             complement(2064..>2413)
                     /locus_tag="PKJ17_07795"
                     /inference="COORDINATES: protein motif:HMM:NF020952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOD75913.1"
                     /translation="LSPGIGRLQRLHGSYVSEGEIKHIVEFLKQQGAPSYHHEILEEK
                     DDEEDGGETIDDEKYQEAVEFVSSRGEASISMVQRRFRIGYNRAARIIERMEQEGVVG
                     PSDGVKPREVLKK"
BASE COUNT          524 a          702 c          659 g          528 t
ORIGIN      
        1 acgaaggatt ctcggttctg aaggccacgg acggcaagga aggccttgcc atcttcgagc
       61 gcgaagcccc cgacgtggtg ctcctcgatg tctggatgcc tgagcttgac ggtatacagg
      121 tcctcaagga gatacgcgcc cggaaggacg acgcgaaggt ggtggtcata tcggggcacg
      181 ggacgatatc gacggcggtg gaggccgtca agatgggtgc ttacgacttt ctcgaaaaac
      241 ccctctccat cgacaaggtg ctcgaggtcg tctcccgcag catcggcgcg ggtttgaaac
      301 atgacccctc tgttgccgat ttcaggctgg aagtggcgcc cggcatcacg agggaaaagc
      361 agaagaccat cggaaagagc accgtcgtct acggggtggg actccactcc ggcgtgaaga
      421 cgggaatgat cctcctgccc atgccggaga atacgggcat agtcttcgag accattcccg
      481 acggggagcg tatacccgcg tacatcgact acgtcttctc cgtggggtac gcgtcgtcag
      541 tgaaaggcaa gaacagtgtc gtgcgcacca tcgagcatct cctgtccgcc tgtcacatgt
      601 acgggatcac caatcttctc gtcaaggtga gcgaagagat acccatcttc gacggttcgg
      661 ccatcgagat ctgcaggaag atcgatgagg ctgagatcgt cgaacagacc gatggtatcg
      721 aaccggtcgt catcacggaa aaggtggagc tccccaatct gcagaacggg aaatacctag
      781 cggcggagcc ctcggagcgc ttcgagatcg actacctcct ggaacagaag aggcccatcg
      841 ggacccagca ttaccggttt gtcgccggcc gcgacgacta cgtcgccaac atcgctcccg
      901 cgaggacctt cggcttcctc aaggactttg aacagcttgc caagatgggt ctcggaaccg
      961 gcgggcgcat caacaatttc atcatcctcg gcgaagacga ggtcataaac acgaagctga
     1021 ggtttgagga cgagttcgtc cgccacaaga tcctcgacct catcggcgac atctaccttc
     1081 tcaaccgtcc cataacgggc aagataaccg cccgacagac aggccacgtg gagaacatcg
     1141 cgctggtaaa ggaactgaaa aaaaggtttc tctgacgaga aaccgtccca agtcgcaggt
     1201 cacaggtctc acgtttcacg tttcaggttc gagccaatac cggaaccttt gcaactgtat
     1261 tggttgggcc agtcaagaaa gggtccctct ttggcctttg acccgcaacc cggaacccgc
     1321 aaccgtcttt cgcctgaaac ctgaaacggt cttttatctt tccaccacat ccttgatgcc
     1381 gtcggacttg taggtgaatt ttgagtcgct gacgggagcg ttcaccttga tgtcggtgaa
     1441 ctcgatcgtg ttgacgttgc cggtaaattc gaggatctcg atcttcctca ggagattgtt
     1501 cttgtcgatc cagatcttgg caatgttgac ggtggaatcc ttcttcggga gaagctcgaa
     1561 gtactgcatc cccgccagca tgctgtgttg cttgacggtg aagaggttgt cgatgcgtgc
     1621 tatgtcctcg acgaggtcaa agaaggtgcc ccccgtcttc tccctgctga ccttgctctt
     1681 gagcacgaag gtcttgcctt cctcatcctg ccacatgtaa tgcccgtcat agaggaacgt
     1741 cttgcccttg ggcttcgtgt acttccagag gaaccctttg tgccgcttga aatagaagtt
     1801 cccgtcgaaa tcgcggatct tcttgatgcc ggaaacgaag atcttctgat gaaaggaggc
     1861 ctccaacgcg gtgatgccgg cataggtctt ctttatggaa tcgaagggag acccggtctc
     1921 gcccttagcg gcggacgtcg cgtccccgcc ggcgaaaaag ggaataagca ggaacaccgc
     1981 cagaacgccg aacaataccg gcgatgacag gtttcttgtt ctccgaagag ctctatcttt
     2041 cattcgacca tacctctcaa ggatcatttc ttcaggacct ccctcggctt cacaccgtcg
     2101 gagggaccca caacaccttc ctgctccatc cgttctatga tgcgggccgc ccggttgtat
     2161 ccaatgcgga acctcctctg gaccatgctg atcgatgcct ctcccctcga tgacacgaat
     2221 tcgacagcct cctgatattt ctcgtcgtct atcgtctcgc cgccgtcttc ctcatcgtct
     2281 ttctcttcca ggatctcgtg atgataggag ggcgccccct gctgcttcag gaattccaca
     2341 atgtgcttga tctcgccttc ggagacatag gacccgtgga ggcgctggag ccgcccgatc
     2401 ccggggctca gga
//
LOCUS       DAOPOA010000359         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_47728_length_2411_cov_3.831919, whole genome shotgun sequence.
ACCESSION   DAOPOA010000359 DAOPOA010000000
VERSION     DAOPOA010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47728_length_2411_cov_3.831919"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1221)
                     /locus_tag="PKJ17_07800"
     CDS             complement(238..1221)
                     /locus_tag="PKJ17_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75914.1"
                     /translation="MTNPWKILLVALTMAGVFCTCAHGADTPRFVFATLEQGREILTA
                     RDVYIEQMSPFDRSCRMKTNKVVTRETFLDFVSNNVLPWDQADKELLGPVLAVIESKL
                     KDFASFLPEGIYLVKTTGNEEGGAAYTRGGAIVIPRAMVGTDRVALCKLLSHELFHVI
                     TRLDPRLRDELYETIGFTKCPPFEFPKTLKDRRITNPDSPLNEHCISVKVGKRRLLVL
                     PILYSRVEEYDRSHSEHLFDYLQLAFLGVARDNGPGRKPTPLRGAKPDLYDESEMSGF
                     FEQVGRNTDYTIHPEEILADNFSYLFFGVTELESPEVVRKMERVFEKARLK"
     gene            complement(1291..>2411)
                     /locus_tag="PKJ17_07805"
     CDS             complement(1291..>2411)
                     /locus_tag="PKJ17_07805"
                     /inference="COORDINATES: protein motif:HMM:NF020477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HOD75915.1"
                     /translation="RGIRVYTHRFTRGYPTDVDMIVSDEGYGANAYMESDRPLVIVTG
                     PGPGSGKLATCLSQLYHDHKRGIRSGYAKFETFPIWNLPLKHPVNVVYEVATADIRDF
                     NLIDPFHMEAYGKTSINYNRDVEVFPVLYRIMEKITGGGSFYRSPTDMGVNRVGFAII
                     DDEVVREASKQEILRRFFRYRCEYAMGFADKETVQKAELFMKEFKLDPEHRRVVTPAR
                     QAAVDAQAANKGNEGIFCGASIELKDGTIITGNNSPLMHAATSLVIHAIKHLAGIPEQ
                     LALLPEYVTESVRNLKTRILNESSVSLDLEEALIVLSVSTMTNPAAQLALEKLKDLRE
                     CEVHMTHIPSPGDEAGLRRIGVNLTSDPSFATKNLFVT"
BASE COUNT          447 a          668 c          730 g          566 t
ORIGIN      
        1 ggacaacgga ctctgactcc gtgaatcaag gttcaaatcc ttgtctccca gccagatcga
       61 aaggggctga ccgaatggtc agcccctttg cctgtctttc gggactcatt actcattcgg
      121 agtcccgccg ctcgcgatga ccttattatg cccgttgccg ccggggcgat gccagcggcc
      181 atgaacaggg cacagagcgc gaaggcatgc aggcaggaca gattccgcaa accgttgtca
      241 cttcaacctt gccttctcaa agacccgctc catcttcctg accacctccg gcgattcaag
      301 ctccgttacg ccgaagaaga ggtaggagaa attgtccgcc agtatttcct cgggatggat
      361 cgtgtaatcc gtgttccttc caacctgttc aaagaagccg ctcatctccg attcgtcgta
      421 gagatccggc ttcgcccccc tgaggggcgt cggtttgcgt ccggggccgt tgtcccgtgc
      481 gaccccgagg aaggcgagct gaagatagtc gaagagatgc tcgctgtggg atctgtcgta
      541 ctcttcgacc cgggagtaga ggatcggcag gacaagaagc ctgcgcttgc cgaccttcac
      601 ggagatgcag tgttcgttca gcggtgagtc ggggttcgtg atcctgcggt ccttcagtgt
      661 cttcggaaat tcgaaaggcg gacacttcgt gaaccctatc gtttcataga gctcatcgcg
      721 caaccggggg tcgaggcgtg ttattacgtg aaagagttcg tgagacaaga gcttgcacag
      781 ggcgaccctg tcggtcccga ccatcgcccg ggggatcacg atggccccgc cgcgcgtata
      841 ggccgcgcct ccctcttcgt tgccggtggt ctttacgagg tagatccctt ccggcaggaa
      901 agaggcgaaa tctttcagct tcgattcgat cacggcaaga acggggccaa ggagttcctt
      961 gtcggcctga tcccatggca ggacgttgtt cgagacgaag tcgaggaatg tctcccgtgt
     1021 gacaaccttg tttgttttca tgcggcagga tcggtcgaag gggctcatct gctcgatgta
     1081 gacgtctctg gcagtcagta tctcccgtcc ctgttcgagg gtggcgaaga cgaagcgagg
     1141 tgtgtcggcc ccgtgggcac aggtgcagaa gacgcccgcc atggtgagag caacaagtag
     1201 tatcttccag ggattcgtca tgcgcgcatt tcctttcctt gggagtttat gacatgggat
     1261 gggtcaggcg tgtcaggtcc cggtgccgtg tcatgtgaca aagaggttct tggtggcgaa
     1321 gctgggatcg cttgtcaggt tgactccgat acggcgcagg ccggcctcgt cgccggggct
     1381 gggaatgtgt gtcatgtgta cttcgcactc gcggagatcc ttgagctttt ccagggcgag
     1441 ctgcgccgcc gggttggtca tggtgctcac ggacaggacg ataagggcct cctcgaggtc
     1501 gagactgaca gagctttcat tcagtatgcg cgtcttcagg ttgcgcactg attcggtgac
     1561 gtattcgggt aacagtgcca gctgttcggg gattcccgcc aggtgcttga tggcatgaat
     1621 gacgagactg gtggcggcgt gcatcaaggg ggagttgttt cccgttatga tcgtcccgtc
     1681 cttgagctca atggatgccc cgcagaatat cccctcgttt cccttgttcg cggcctgcgc
     1741 gtcgacggcg gcctgccggg ccggtgtgac gacccggcgg tgttcgggat cgagcttgaa
     1801 ctccttcatg aaaagctcgg ccttctggac ggtctcctta tcggcgaacc ccatggcgta
     1861 ttcacagcga tagcggaaga acctgcgaag gatctcctgt ttcgatgcct ccctgacaac
     1921 ttcgtcatcg atgatcgcga agcctaccct gttcacaccc atatccgtgg gtgacctgta
     1981 gaacgatccg cctcccgtta tcttttccat gatcctgtac agtacgggaa agacctcgac
     2041 atcgcggttg taattgatgg atgtcttgcc gtatgcctcc atgtgaaagg ggtcgatgag
     2101 gttaaagtcc ctgatgtctg cggtggccac ttcgtagacg acgttgacgg ggtgcttgag
     2161 gggcagattc cagatgggga aggtctcgaa cttggcgtat cccgaccgta tgccccgttt
     2221 atggtcgtgg tagagttggg agaggcacgt tgccagcttc ccgcttcccg gtcccggacc
     2281 ggtgactatc acaagaggcc tgtcggattc catgtatgca ttggccccgt aaccttcgtc
     2341 gctgactatc atgtccacat cggtgggata ccccctcgtg aatcggtgcg tgtacaccct
     2401 gattccccgt c
//
LOCUS       DAOPOA010000360         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_48007_length_2399_cov_3.321246, whole genome shotgun sequence.
ACCESSION   DAOPOA010000360 DAOPOA010000000
VERSION     DAOPOA010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48007_length_2399_cov_3.321246"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="PKJ17_07810"
     CDS             <1..1310
                     /locus_tag="PKJ17_07810"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75916.1"
                     /translation="TFNVLLFSGASRLMSEKSLPATKENVDNAINVIDRQRGGGGTEL
                     LPALDRVLKLPKFDNYSRSIIIVTDGYVMVEEEVFDLIRTHLNDANVFAFGIGSSVNR
                     HIIEGMARVGMGEPFVITKPADAPAQADRFRAMIESPVLTKVKVSFKGFSTYDVEPVS
                     IPDVLADRPVMVFGKYRGEAKGTIMVTGVSGNGRFTESINVAAAKPLKENVALRYLWA
                     RHRITLLSDYNKLHSSDKRVKEVTGLGLAYNLLTAYTSFVAVDNQVRNTGDKPQTVNQ
                     PLPLPEGVSDYAVGGSGMMKTMAMAPPSPSQYMRPGYMGAARAEEAPMKDSGKRKAPA
                     GPRVSLGDVTVSGGIAKQAVLEAMRKIEGDIAACAAGISSPVSYVVTLIVNADGTVKD
                     AKFKDGKNNSQEQCIRSLMKQLKFTATRDGKEASVTVTILLAP"
     gene            1402..1947
                     /locus_tag="PKJ17_07815"
     CDS             1402..1947
                     /locus_tag="PKJ17_07815"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOD75917.1"
                     /translation="MIEEWELLESRVDRDYRVFRIQSELAMSPRTKKVGKFYTIDTRD
                     WVNVIPVTPEGQIVMIKQYRHGSKEVTLEIPGGLVDEEDPREAAQRELLEETGYAGDR
                     VELLGFVNPNPAIFNNRCHTYLIENAREISEKALDPDEDIEVVLMSADEIPSIIAGGG
                     INHSLVIVAFHFYFMKKLCSQ"
     gene            1944..>2399
                     /gene="mobB"
                     /locus_tag="PKJ17_07820"
     CDS             1944..>2399
                     /gene="mobB"
                     /locus_tag="PKJ17_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOD75918.1"
                     /translation="MSARSQKVFVLGVAGRSGSGKTTLIERLLPLLKGQGLRVAVIKH
                     THHDLEFDVPGKDSYRFKQAGAVTSVLVSPTGLGIVKDFREEPSLDEIISRYATDCDM
                     IIVEGFKHERAPKIEVFSADRGEPPLCLSGDETYIAIVTDEDIDTPLPRF"
BASE COUNT          534 a          725 c          703 g          437 t
ORIGIN      
        1 ataccttcaa cgtcctgctc ttctcgggcg catcgaggct catgtccgaa aagtccctcc
       61 cggcaacaaa agaaaacgtg gacaacgcca tcaacgtcat cgaccgccag cgcggcggtg
      121 gcgggacgga gcttctcccg gcgctggacc gcgtcctcaa gctcccgaaa tttgacaact
      181 actcccggag catcatcatc gtgaccgacg gttacgtcat ggtcgaagag gaggtgttcg
      241 acctcatccg cacccacctc aacgacgcca acgtgttcgc cttcgggata ggcagctccg
      301 tgaaccgcca catcatcgag ggcatggccc gtgtgggcat gggcgagccc ttcgtcatca
      361 cgaagcctgc tgacgcaccg gcccaggcgg accgcttccg ggcgatgata gagtcgcctg
      421 tcctgacaaa ggtcaaggtg agcttcaagg ggttctccac ctatgacgtg gagcctgttt
      481 ccattcccga tgttctcgca gaccgccccg tgatggtctt cggcaagtac aggggagaag
      541 ctaaaggcac cataatggtg acgggtgttt cggggaacgg gagattcacg gagtccatca
      601 acgtggcggc cgcgaaaccg ctgaaggaga acgtcgcgct gcggtatctc tgggcgcgcc
      661 accggatcac gctcctctcg gactacaaca agcttcattc cagtgacaag cgtgtcaagg
      721 aagtgaccgg gctcggcctc gcatataacc tcctcacggc ctacacatcc ttcgtggccg
      781 tcgacaacca ggtgaggaac accggggaca aaccccagac ggtgaaccag ccgctgcccc
      841 tgcccgaagg cgtttccgac tatgcggtcg gcggctccgg catgatgaag accatggcga
      901 tggccccgcc ctctccgtcg cagtacatgc gcccgggcta catgggagcg gcccgcgctg
      961 aagaggcccc gatgaaggac agcgggaaac gaaaggcccc tgccggcccc agggtctccc
     1021 tcggtgacgt gacggtttcg ggcggcatcg ctaagcaggc ggtgctggaa gccatgcgga
     1081 agatcgaagg cgacattgcg gcatgtgccg ccggcataag cagccccgtc agctatgttg
     1141 tcaccctgat agtgaatgct gacggcacgg tgaaggatgc gaagttcaag gatgggaaga
     1201 acaattccca ggagcagtgc ataaggtcac tcatgaaaca gctcaaattc acggcgaccc
     1261 gggacggcaa ggaggcatcg gtgacagtca cgatacttct cgcgccctga gccaaacaga
     1321 cactgcatgc aaggccgggt cgtcagatcc ggccttgcat gttgcattta cctcccgccg
     1381 cgtggtattt tgttatccgt tatgatcgag gaatgggaac ttcttgaatc cagggtggac
     1441 agggactatc gcgtgttccg catacagtcg gagcttgcca tgtctccgag aacgaagaag
     1501 gtgggcaagt tctacaccat cgacacccgc gactgggtca acgtcatccc tgttacaccc
     1561 gagggccaga tagtgatgat caagcagtac cggcacggtt ccaaggaggt gacactcgag
     1621 ataccggggg ggctcgtgga tgaggaagac ccccgggaag cggcacagcg ggaattgctc
     1681 gaggagacgg gatatgccgg cgacagggtc gaactcctcg ggttcgtgaa cccgaacccc
     1741 gccatattca acaaccgctg ccacacgtac cttatagaga acgcgcggga aatatcggag
     1801 aaggcccttg accccgatga agatatcgag gtggtgctca tgtccgccga cgagataccg
     1861 tccatcatag cgggcggcgg cataaaccac tccctcgtga tagtcgcctt tcatttctat
     1921 ttcatgaaga aactctgttc ccaatgagtg cgaggtcgca aaaggtcttc gtgctgggcg
     1981 tcgcgggcag gtcgggcagc ggcaagacca cccttatcga aaggctcctt cccctgctca
     2041 agggtcaagg tcttcgcgtc gccgtcatca agcacaccca tcacgacctc gagttcgacg
     2101 ttcccggaaa ggattcctac cgtttcaagc aggcgggagc ggtcacgtcc gttctcgtct
     2161 ccccaacggg actggggatc gtgaaagact tccgggaaga accctccctt gacgagatca
     2221 tctcccgcta tgccacggac tgcgacatga ttatcgtgga agggttcaag catgaaaggg
     2281 cgccgaagat agaggtcttc agcgccgacc gcggcgaacc cccgctatgc ctctccggtg
     2341 acgaaacgta catcgccatc gtcacggacg aagacataga cactcccctt cctcgcttc
//
LOCUS       DAOPOA010000361         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_48420_length_2382_cov_3.685862, whole genome shotgun sequence.
ACCESSION   DAOPOA010000361 DAOPOA010000000
VERSION     DAOPOA010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48420_length_2382_cov_3.685862"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PKJ17_07825"
     CDS             complement(<1..753)
                     /locus_tag="PKJ17_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOD75919.1"
                     /translation="MKIAGIQFACTRDKEKNVNTAVKLLSAALNENAKIVCFQELFNQ
                     YWFPKDRDDAAFALAENADGDTVRLFKSLVKGTDTVIVLPIFEKAGEHHYNSTVVIEG
                     DRTLGTYRKLNVTDIPLWEEKFYFEQGDTGLPVFETTHGKFGIQTSWDNFFQEGTRIL
                     ALKGADIIFAPTSCAFKSQHIWQSVISSNAITNGVYVMRVNRAGSEESQDFYGMSFCV
                     NPEGELIGGPTGRSDTVLLADLDLDRLKLVRRE"
     gene            complement(1034..1609)
                     /locus_tag="PKJ17_07830"
     CDS             complement(1034..1609)
                     /locus_tag="PKJ17_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOD75920.1"
                     /translation="MKSVKGTRTEKNLLASFAGESQARMRYTYFSSQAKKEGYEQISF
                     IFSDTADNEKEHAKRFFKFLEGGEVEITAAYPAGVIGKTTENLKAAAAGENLEHTVLY
                     PEAAKVADEEGFPEIAIVFREIARVEEEHEKRYLKLLKNVETEQVFKKPSVVKWRCRN
                     CGYVYEGAEAPDPCPACDHPQAYYELLAENY"
     gene            complement(1623..1997)
                     /locus_tag="PKJ17_07835"
     CDS             complement(1623..1997)
                     /locus_tag="PKJ17_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOD75921.1"
                     /translation="MAERLQVYKCEICGNIIEVLHGGKGVLVCCNEPMKLLAENTVDA
                     SKEKHVPVIEKGADGITIKVGSVAHPMEEKHYIEWIEAIVDGAVYRRFLKPGEAPEAS
                     FPVKGGSVTAREFCNVHGLWKN"
     gene            2197..>2382
                     /gene="tatB"
                     /locus_tag="PKJ17_07840"
     CDS             2197..>2382
                     /gene="tatB"
                     /locus_tag="PKJ17_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HOD75922.1"
                     /translation="MFGIGLPELILIMIVALLVVGPKKLPELARSIGKALHQVKSMTD
                     DVKQSFEEVTSDDLEEPR"
BASE COUNT          483 a          693 c          631 g          575 t
ORIGIN      
        1 ctcgcgacgg acgagcttca gccggtcgag gtcgaggtcg gcgagaagga ccgtgtcgga
       61 cctgcccgtc ggtccgccga tcaactcacc ctcgggattg acgcagaagc tcataccgta
      121 gaaatcctgg gactcttcac tgcccgcccg gttcacgcgc atgacataca ctccattcgt
      181 gatcgcgttg gacgagatga cgctctgcca tatgtgctga gacttgaagg cgcacgacgt
      241 gggggcgaag atgatgtcgg ccccttttaa cgccagtatc cttgtgccct cctgaaagaa
      301 gttgtcccat gatgtctgta tgccgaactt gccgtgggtc gtctcgaaaa cgggaagccc
      361 cgtatccccc tgttcgaagt agaatttctc ttcccacagc gggatgtccg tcacattaag
      421 ctttcggtat gtcccgagcg tcctgtcgcc ctcgatcacg accgtagaat tgtaatggtg
      481 ctcgcccgct ttctcgaaga tgggcagaac gatcaccgtg tcggtgcctt tgacgaggga
      541 tttgaagagc ctgaccgtat caccgtcggc attttccgcc agggcaaagg ccgcgtcatc
      601 ccggtctttc gggaaccagt actgattgaa cagttcctga aagcagacga tcttcgcgtt
      661 ctcgttcaga gctgcactga gcagcttgac cgcggtgttg acattctttt ccttgtccct
      721 ggtgcaggcg aattgtattc cggctatctt catgaacgtt ctctgacgac atccccctcg
      781 atccacccgg cggtacctgc acgcctgaac tcgctgaaag gcgggccggg caggaagata
      841 atatcataaa acgaggggtt tgtgccgcaa atctccttga ttcttctgga atttgtttaa
      901 tgcaaagaaa ggtaccagat gaaaatgttc tatgtcaagg aaaaataagg ggaaaaaagc
      961 gaaaaggccg tgtgcgctga aggaatcgga taaaggtcct catcccgcgc acacggaccc
     1021 ggtataaccg atatcagtaa ttctcagcca ggagctcgta gtacgcctgc gggtggtcgc
     1081 acgccgggca gggatcggga gcctcggccc cctcatacac gtagccgcag ttcctgcagc
     1141 gccatttgac aacggagggt ttcttgaaga cctgctcggt ctccacgttc ttcaggagct
     1201 tgagatagcg cttctcgtgc tcctcttcga cgcgggctat ttcccgaaag acgatggcta
     1261 tttccggaaa gccttcctca tcggccacct tcgccgcttc gggatagaga acggtatgct
     1321 ccaggttctc gccggccgcc gcggccttca ggttctccgt cgtcttcccg atgaccccgg
     1381 cagggtaggc ggcggtgatc tccacctcgc ctccttcgag gaacttgaag aagcgtttcg
     1441 cgtgctcctt ttcgttgtcc gccgtatcgg aaaaaataaa ggatatctgc tcgtagcctt
     1501 ccttctttgc ctgggatgaa aaataggtat acctcatccg tgcctgcgac tctccggcga
     1561 agctcgcgag gaggttcttc tcggtgcgtg ttccctttac gctcttcatc actttctctc
     1621 ctctagttct tccacagacc gtggacgttg cagaactccc tcgccgtcac ggaaccgcct
     1681 ttcacgggaa aggaggcctc aggtgcctcc cccggcttga gaaagcgccg gtacacggcc
     1741 ccgtcgacga tcgcctcgat ccattctatg tagtgtttct cttccatggg atgggcaacg
     1801 ctgccaacct ttatcgtgat cccgtccgct cccttttcga tcaccggcac atgtttctcc
     1861 tttgaagcat cgacggtatt ctccgcgagc agcttcatgg gctcgttgca gcacacgagc
     1921 actccctttc cgccgtggag cacttcgatg atgttcccgc atatctcaca cttgtatacc
     1981 tgaagtctct cagccacgtg cgcctccttt ttcgatgaat tgaaccgcat atcacaacaa
     2041 tgatggtttc aggttaatac acggcatcgg tgatgtcaat ggtgcggccg ctgtgggcac
     2101 aagggggcgg ggaggcctcc cggtgccatc gtcctgacag gggggaaagg cacaattctc
     2161 ttttaagacc gttatttttg tgatagttta tccccaatgt ttggtattgg tctccccgaa
     2221 ctcattctca taatgatcgt cgcactcctg gtggtagggc cgaagaaact gcccgaactc
     2281 gcacggtcca tcggcaaggc ccttcatcag gtcaagagca tgaccgacga tgtgaagcag
     2341 tccttcgagg aggtcacgtc agacgatctt gaagaacccc gg
//
LOCUS       DAOPOA010000362         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_48587_length_2376_cov_3.213701, whole genome shotgun sequence.
ACCESSION   DAOPOA010000362 DAOPOA010000000
VERSION     DAOPOA010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48587_length_2376_cov_3.213701"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..909
                     /gene="pyrF"
                     /locus_tag="PKJ17_07845"
     CDS             172..909
                     /gene="pyrF"
                     /locus_tag="PKJ17_07845"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOD75923.1"
                     /translation="MMESAMDPREKMIFALDVDRFEEAQQLVMEFKDHVGMFKVGKQL
                     FTQCGPKIVDYIKLKNSKVFLDLKYHDIPNTVAKASVEAAKLGVDILNVHASGGFAMM
                     SEAKRALVEAGKNPGLVRPRIIGVTVLTSLDDAELKRVGFDMPVIELTKRLALLAKEA
                     GLDGVVAGGSEIEMIRETCGRDFLIITPGVRVEDRKDDQKRTITPQEAIRRGASHIVL
                     GRAVRDAADPKALLRKITGDIRDALSV"
     gene            893..1612
                     /locus_tag="PKJ17_07850"
     CDS             893..1612
                     /locus_tag="PKJ17_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOD75924.1"
                     /translation="MPFLFNRNSIREALKNHPGKVRRLLIRQGHEKLSEELIEEAKRQ
                     GVSYRVLPSEAFAKKCGSPRSHVCLEREDFDYTGQDTFLDRLGSMGPVFLGALDGVQD
                     PQNLGNIARSSACMGVDALILPKDRSCVMNETVANISRGATELIEAVRVTNLSRYLDA
                     LKKRGVFCYGLDERGATALWEVDLTGSVCLVFGGEEGLRRLTKETCDALVRIPTNPSF
                     PSLNVATSFALACYEVVRQRL"
     gene            1679..>2376
                     /locus_tag="PKJ17_07855"
     CDS             1679..>2376
                     /locus_tag="PKJ17_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOD75925.1"
                     /translation="MNLTKVFQEKGVRDLLSESWSVSAPMTLIMVFEFLIGITDIYVA
                     GKVGKEIQATYGFVIQLYFVFIIIANALTTGTVSVLSRLFSSGDRKELGRAIFSVLVV
                     AAAAGLIFGILGIVLTPRLIAIVSIPTSLKPLGSPLGQIYAAGLIFHYMLINTNGVLR
                     SCKSVRLSLTTMATVCVSNICLNFFLVFGTPLGYTGIALATVLSTFAGCIMNLRHLRS
                     FVPSSAMAFAAGRI"
BASE COUNT          507 a          672 c          712 g          485 t
ORIGIN      
        1 aggttgacgg gggaaaggtc gttgaggaga tacttgacgg ccacaaggtt cgtgccccag
       61 atggcactga cacagaggag cagaatatct gacaggcgtt cacgttccac gcagttattc
      121 ttaattgccg tgaaatgctt tgtcaatcgt taaccgcgag aagtcacaat aatgatggag
      181 agtgcgatgg accccaggga aaaaatgatc tttgctctcg atgtagaccg tttcgaggaa
      241 gcgcagcagc ttgtcatgga gttcaaggac cacgtgggca tgttcaaggt gggcaagcag
      301 ctctttacgc aatgtggacc gaagatagtc gactacataa agctgaagaa ctcgaaggtc
      361 tttctcgacc tcaagtatca cgacatcccc aataccgtgg ccaaggcttc cgtcgaggcg
      421 gcgaagctcg gggtcgacat cctgaacgtc catgcctcgg ggggattcgc catgatgagc
      481 gaggccaagc gggccctcgt cgaggcgggc aagaaccccg gcctcgtgcg tcccaggatc
      541 ataggggtaa cggtcctcac gagtctcgat gacgcggaac tgaaaagggt cggcttcgac
      601 atgcccgtca tcgaactgac aaaaaggctg gcgcttctcg cgaaggaggc cgggctcgac
      661 ggcgtcgtag cggggggaag cgagatcgag atgatcaggg agacctgcgg cagggacttc
      721 ctcatcatca cacccggggt gagggtcgaa gacaggaagg acgaccagaa acggacgatc
      781 acaccgcagg aggcgataag gaggggggcc tcgcacatcg tccttggcag ggcggtcagg
      841 gacgcggcgg acccgaaggc cctcctgaga aagatcaccg gggatatacg cgatgccctt
      901 tctgtttaac aggaacagta tcagggaggc cttgaagaac catccgggca aggtccgcag
      961 gcttctcata cggcaaggtc acgagaaact ctcggaggaa ttgatcgagg aggcgaaaag
     1021 gcagggggta tcgtaccgtg tcctgccctc cgaggccttc gcgaagaagt gcggctcccc
     1081 gaggtcgcac gtgtgtctcg aacgcgagga cttcgattat accgggcagg atacctttct
     1141 cgatcgtctc ggttcaatgg gtcccgtgtt cctgggcgcg cttgacggcg tgcaggaccc
     1201 ccagaacctg gggaatatcg cgcgaagctc cgcctgcatg ggggtcgacg cccttatcct
     1261 tcccaaagac cgttcctgcg tcatgaacga gacggtggcc aacatatccc ggggggccac
     1321 cgaattgata gaggccgtga gggtcaccaa cctttcgcgg tatctcgatg ccctgaagaa
     1381 aaggggggtc ttctgctacg gtcttgacga acggggcgcc acggcactgt gggaggtaga
     1441 tctcacggga tctgtatgtc ttgttttcgg cggcgaagag ggcttgcgca gactgaccaa
     1501 ggaaacctgt gacgccctcg tgaggatccc gacaaaccct tcgttcccgt ccctcaatgt
     1561 tgccacgtcc tttgcccttg cctgttatga ggtggtcagg caaagactgt gacgggccct
     1621 ctccagtgag ggtggcaccg acctttttcg ccggaacccg gatcgcgctg atcagagtat
     1681 gaacctgaca aaggtttttc aggaaaaagg ggtccgcgac ctcctctccg aaagctggtc
     1741 ggtgagcgcc cccatgacgc tcatcatggt tttcgaattc ctcatcggga tcacggacat
     1801 ctatgtcgcc ggcaaggtcg gcaaagagat acaggcgaca tacggcttcg tcatccagct
     1861 ctacttcgtc tttattatca tcgccaacgc cctgacgacg ggaacggtat cggtcctctc
     1921 ccggctcttc agctcaggcg acaggaaaga gctcggaagg gccatcttct ccgtccttgt
     1981 cgtcgctgcg gccgcgggcc tcatctttgg tatcctgggc atcgtcctca cacccaggct
     2041 catcgccatc gtgagcattc cgaccagtct caagccgctg gggtcaccgc tcgggcagat
     2101 atacgccgcg ggcctcatct ttcattacat gctcatcaat acgaacggcg tgctgcgctc
     2161 ctgcaagagt gtgcgccttt ccctgacaac gatggcgaca gtctgcgtct ccaacatatg
     2221 cctcaatttt ttcctcgtct tcggcacacc tctcggttac accgggattg cgctggccac
     2281 ggtcttgagc accttcgcgg gatgcatcat gaacctgcgt catctgcgtt ccttcgtgcc
     2341 atcgtcagcg atggcgttcg cggcgggacg tatcag
//
LOCUS       DAOPOA010000363         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_48790_length_2369_cov_2.791703, whole genome shotgun sequence.
ACCESSION   DAOPOA010000363 DAOPOA010000000
VERSION     DAOPOA010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48790_length_2369_cov_2.791703"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKJ17_07860"
     CDS             complement(<1..680)
                     /locus_tag="PKJ17_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD75926.1"
                     /translation="MKDDLNIVGKRVVRSDSLAKVTGEARYTADLTFPGMLVAKVLRS
                     PYPHAKIVGIDVSKALKVPGVKAAISGFDSYGVKWGVFRYTQDHAMLHTDKVRYIGED
                     VAAVAAVDEETAMEALARIKVDYEPLPAVYDPMESMKDGAPQIHDQYKNNINIHIHID
                     VGEVDRVMEKAYLVREDTFKAAGEAYAMMEPYAVVACFKSGYLDLYMPNAGPHVRAKA
                     LSNMLRLPL"
     gene            complement(692..1951)
                     /locus_tag="PKJ17_07865"
     CDS             complement(692..1951)
                     /locus_tag="PKJ17_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD75927.1"
                     /translation="MKEAPKLNIKRSIELFEEAKTLVPGGVLGARKPGDFIMGEYPIF
                     LEYGKGSRLIDVDGNEFIDFLCGYGPIILGYREDEVDDAVIRQLKDKGFCFSLTQPYQ
                     NELARKLNALIPSAELSIFLKTGSDATTASIRIARAYTGRTKVMRSGYHGWHDWCVEM
                     KGGIPEKFYEDVYEFHYNDLEALEDLMKKHGDQTAAIIMTPFGHPLHQKMQTPKPGFL
                     EGVRKLADTYGAVLVFDEVRTCFRLRMGGAQELYGVKPDLTVLGKGMANGYAISVVCG
                     KTDVMMAAASKLFISSTFFPNSDGYIAALKVIEIMEREKVLDQIWEKGERLLKRIQGV
                     IDKYPNTGAELTGVAPMFHVTFTKGDPDTQRDRRTDFYTQMIRKGFFFTPHHHAYLSY
                     RHTEEDLDLTIQAMDESMAYVSEKYKG"
     gene            complement(1983..>2369)
                     /locus_tag="PKJ17_07870"
     CDS             complement(1983..>2369)
                     /locus_tag="PKJ17_07870"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOD75928.1"
                     /translation="QKGMGLLGSEPVEVRGGKVVPYDLALRLWDTIPKDRDNGPQASG
                     LKVIVKGERQGKQVTYTADIVGRMAPGTGLPASIAALMMDAGEVTVKGVVAPEGCIDP
                     DRFLAELLKRGARIHQTETIRSMFEL"
BASE COUNT          422 a          725 c          681 g          541 t
ORIGIN      
        1 aggggcagcc tcagcatgtt cgacagggcc ttggcccgga cgtgcggccc cgcgttcggc
       61 atgtagaggt cgagatagcc actcttgaaa caggcgacca cggcgtatgg ttccatcatg
      121 gcataggcct cgccggccgc cttgaaggtg tcctcccgga caaggtacgc cttctccatg
      181 accctgtcga cctcgcccac gtcgatatga atatggatgt tgatgttgtt cttgtactgg
      241 tcatggatct gtggtgcacc gtccttcatg gactccatgg ggtcgtaaac ggcagggagg
      301 ggctcatagt ccaccttgat acgggcgagg gcctccattg ccgtctcctc gtcgacggca
      361 gccaccgccg cgacgtcctc gccgatgtac cgcaccttgt cggtgtggag catggcatgg
      421 tcctgagtat acctgaaaac accccacttt acaccatagg agtcgaagcc gctgatggcg
      481 gccttgacgc ctggcacctt gagggccttc gaaacatcga tgcccacgat cttcgcgtgg
      541 ggatacgggc tcctcaagac ctttgccacg agcatccccg gaaacgtaag gtcggccgta
      601 tagcgcgcct cgcctgtcac cttcgccagg gaatcgcttc gaacaaccct tttgcctacg
      661 atattgagat cgtccttcat taacgtcccc cctacccttt atacttctcg ctcacatagg
      721 ccatggattc atccatcgcc tggatcgtga gatcgaggtc ctcttcggtg tgcctgtagc
      781 tgaggtaggc gtggtgatgg ggtgtgaaga agaacccttt cctgatcatc tgggtgtaga
      841 agtcggtcct gcggtctctt tgcgtgtcgg ggtcaccctt cgtgaaggtc acgtggaaca
      901 tgggggccac gcccgtgagt tccgccccgg tgttcggata cttgtcgatg acaccctgga
      961 tccttttgag gagccgttcg cccttttccc atatctggtc gagcactttc tcacgctcca
     1021 tgatctcgat gaccttgagc gcggcaatgt agccgtcgct gttcgggaag aacgtggagg
     1081 agatgaaaag cttcgatgcc gcggccatca tcacgtccgt tttcccgcag accacactga
     1141 tggcataccc gttggccatg cccttgccga gcacggtgag gtccggtttg accccgtaaa
     1201 gttcctgcgc cccgcccatc ctgaggcgga aacaggtgcg cacctcgtcg aagacaagga
     1261 ccgcgccgta ggtgtccgcc agcttgcgca caccctcgag gaagcccggt ttcggcgtct
     1321 gcatcttctg gtggagaggg tggccgaaag gcgtcatgat gatggccgcc gtctggtccc
     1381 cgtgcttctt catgaggtct tcgagcgctt cgaggtcatt atagtggaat tcgtagacat
     1441 cctcatagaa cttctcggga atgcccccct tcatctcgac gcaccagtca tgccagccat
     1501 ggtacccgga gcgcatgacc ttggtgcgtc cggtgtaggc cctcgcgata cggatgcttg
     1561 ccgtcgtcgc gtcggagccg gtcttgagga agatgctcag ctcggccgac gggatgaggg
     1621 cgttgagctt cctcgccagc tcattctgat agggctgggt aaggctgaag cagaacccct
     1681 tgtccttcag ctgccggatg acggcgtcgt cgacctcatc ctcgcggtaa ccgaggatga
     1741 tggggccgta gccgcagagg aaatcgatga actcgttccc gtcgacgtcg atgagccggg
     1801 aacccttccc gtactcgaga aagatgggat actcgcccat gatgaaatca cccggtttcc
     1861 tggctcccag gaccccgccc gggacgaggg tctttgcctc ctcgaagagt tcgatacttc
     1921 tctttatgtt caacttcggg gcttctttca tatgaacctc cttcttaggg aacctgtcgg
     1981 cattaaagct cgaacatgga ccggatcgtc tcggtctggt gtatccgcgc gcctctcttc
     2041 agcagttccg caaggaaccg gtcggggtcg atgcagccct cgggggccac gacacccttg
     2101 acggtgacct cgccggcatc catcatgagg gccgctattg acgcgggaag acccgttccg
     2161 ggggccatcc tgccgacgat atcggccgtg taggtcacct gcttcccctg tctctctcct
     2221 ttcactatga ccttgaggcc cgaagcctgg ggaccgttgt ccctgtcttt cgggatggta
     2281 tcccagagtc tcaaggcaag gtcgtagggt acaaccttcc ctcccctgac ctcgacaggt
     2341 tcgctgccga ggagtcccat acccttctg
//
LOCUS       DAOPOA010000364         2368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_48821_length_2368_cov_2.813662, whole genome shotgun sequence.
ACCESSION   DAOPOA010000364 DAOPOA010000000
VERSION     DAOPOA010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48821_length_2368_cov_2.813662"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1414
                     /gene="trmFO"
                     /locus_tag="PKJ17_07875"
     CDS             128..1414
                     /gene="trmFO"
                     /locus_tag="PKJ17_07875"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOD75929.1"
                     /translation="MDITVIGGGLAGVEAAHQITRMGGQATLLEMRPGAGTPAHRTSH
                     LAELVCSNSLKSLDLHNAHGLLKEELRRMGSVIMDAADGTAIPGGKALVVDRDRFAAR
                     LTQTVENDPGIRLVREEALRIPEEGIVVVASGPLTSEALAEELRALTGADNLAFFDAI
                     SPIVDAASIDHGKAFYASRYMKGEAGDYLNCPLTREEYDLFHEALVSAERVDLREFEK
                     TSYFEGCLPVEVLAERGRKTLAFGPMKPVGLTDPRDGKRPYAVVQLRKENASGTMLNL
                     VGFQTKMKYAEQERVFRMIPALKNAVFLRYGSIHRNTYINAPLCLNDRLELKADRRVL
                     FAGQITGVEGYMESTAMGLAAGISAFMSATGREFCPPPPVTCIGALIRYLTTENRNFQ
                     PMNINFGLIEGYSKKNKEATVDRALQEIEKWKNSFK"
     gene            1634..>2368
                     /gene="bioB"
                     /locus_tag="PKJ17_07880"
     CDS             1634..>2368
                     /gene="bioB"
                     /locus_tag="PKJ17_07880"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOD75930.1"
                     /translation="MTSLFDTLADRAIATRDISVKDALELYEAGKQDPFGLMARGSRI
                     REHFKGKTVNLCGIVNAKSGVCPENCRFCAQSAHYHTDAPVYPLISAEDIAEKGRAAK
                     ESGAHMFSIVTSGTRIETEAEWETIYRAIAMLSEMGLKPCGSLGMLSEETAGNLKKAG
                     LYRYHHNLETSRSFFDSICSTHEYDEDTATVRIARAAGLTVCSGGIIGLGEAMEHRIE
                     MAMTLRELAVDSVPINILNAIPGTPLE"
BASE COUNT          550 a          738 c          670 g          410 t
ORIGIN      
        1 gctcggaata ccccaggtgc tccttcgcca caaacaagga gccgacggag ggtgaatgcc
       61 cctcctgcgg cgccccgacc ctgttctcct tcaagaaggg tctcttttgt cttcgcaagg
      121 attgcggatg gacatcacgg taatcggggg cggcctcgcg ggtgtcgagg ccgcgcacca
      181 gatcacccgg atggggggac aggcaactct tcttgagatg cgccccggcg ccggcacgcc
      241 cgcccaccgg acctcacacc tcgcggagct cgtctgcagc aattccctca agtcgctgga
      301 cctccacaac gcccacgggc tcctcaagga agagctgcgc cggatgggtt ccgtcatcat
      361 ggacgcagca gacggcacgg ccatacccgg cggcaaggcg ctcgtcgtcg acagggaccg
      421 gttcgccgca cggctcacgc agactgtcga gaacgatccc ggtatccggc tcgtccgcga
      481 agaggcgctt cggataccgg aagaaggcat cgtggtcgtc gcgtcgggtc cgctgacaag
      541 cgaggccctc gccgaggaac tgagagcgct tacgggcgcg gacaacctcg cgttcttcga
      601 cgccatatcc cccatagtcg acgccgccag catcgatcac ggcaaggcct tctacgcgtc
      661 ccgctacatg aagggcgaag caggggacta tctcaactgc cccctcacga gagaggaata
      721 cgacctcttc cacgaggccc ttgtgagcgc cgaaagggtc gatctcaggg aattcgagaa
      781 gacgtcctat ttcgagggtt gtcttcccgt ggaggtcctc gcggagcggg gcaggaaaac
      841 actcgccttc gggcccatga aacccgtggg acttacggac ccacgcgacg gaaaacgtcc
      901 ctacgccgtg gtgcagctca gaaaggagaa cgcctcgggg accatgctca acctcgtcgg
      961 ttttcagacg aagatgaagt acgccgagca ggagcgggtc ttcaggatga tacccgccct
     1021 gaagaacgcc gtttttctcc gatacggcag catccaccgg aacacctaca tcaacgcccc
     1081 cctgtgcctg aacgacaggc tcgagctcaa ggccgaccgg cgggtcctct tcgccgggca
     1141 gataacaggg gtcgaggggt acatggaatc gacggcaatg ggcctagccg cgggcatctc
     1201 cgcgttcatg tccgcgacgg gcagggaatt ttgccccccg cctccggtta catgcatcgg
     1261 cgccctcatc cgctacctca ccaccgagaa caggaacttc cagcccatga acataaactt
     1321 cggccttatc gagggctatt cgaaaaagaa caaagaggca acggtcgaca gggcgctgca
     1381 ggaaatagag aaatggaaaa atagttttaa gtgataaaaa cagttttaag ttttaggttt
     1441 taagtgttaa gttaagaaaa acagttttaa gtgagaagaa cggttttaag ttttaagtaa
     1501 aagactgaaa agaaagagac gagatagcgg agtgctccgg cattactctc accgcataaa
     1561 ggaaccgatt ctccctgcct ttaacttaaa acctaaaact taacacctaa aacggtctct
     1621 gataggagct attatgactt ccctttttga cactctggcc gatcgcgcga tcgcgaccag
     1681 agatatatct gtgaaagacg ccctggaact ctacgaagca ggaaagcaag accctttcgg
     1741 cctgatggcc cgggggtccc ggatacggga gcacttcaag ggcaagaccg taaacctctg
     1801 cgggatcgtg aacgcgaagt ccggcgtctg ccccgagaac tgccgtttct gcgcccagtc
     1861 ggcgcactac cataccgatg cgcccgtcta ccccctcata tccgctgagg acatcgcaga
     1921 gaaagggcgg gccgcgaagg agagcggcgc ccacatgttc agcatcgtca ccagcggaac
     1981 gaggatagag acggaagccg agtgggagac catctaccgc gccatagcga tgctctccga
     2041 gatggggctc aaaccctgcg gctcccttgg aatgctgagc gaggaaaccg ccggaaacct
     2101 caagaaggcc ggcctctacc gctaccacca caacctcgag acatcgcgaa gcttcttcga
     2161 cagcatctgt tcgacccatg aatacgacga agacaccgcc acggtgcgca tcgcccgggc
     2221 tgccggcctc accgtctgct ccggcggcat catcggcctc ggcgaggcca tggagcaccg
     2281 catcgagatg gccatgaccc tccgggaact cgccgtggat tccgttccca tcaacatcct
     2341 caacgccatc cccggcacgc ccctcgag
//
LOCUS       DAOPOA010000365         2368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_48827_length_2368_cov_2.516645, whole genome shotgun sequence.
ACCESSION   DAOPOA010000365 DAOPOA010000000
VERSION     DAOPOA010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48827_length_2368_cov_2.516645"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKJ17_07885"
     CDS             complement(<1..514)
                     /locus_tag="PKJ17_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD75931.1"
                     /translation="MARFEEFRGKALVFLLFLLFVWFVSFSIRIVFSPILPLVEDEFG
                     VHHAKASAIFTFLSAGYGLSVILSGLLSGRVGYKRTIVLSLSLLSILAFLIPAVHTFY
                     LLYFFAFFLGFAHGMYIPSAIPLITEYYTEKNWGKAIAIHDTGASLAIFATPLVVLFL
                     LHFFPWRGIFV"
     gene            682..840
                     /locus_tag="PKJ17_07890"
     CDS             682..840
                     /locus_tag="PKJ17_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75932.1"
                     /translation="MAEATYICRRCGITFSAIPGEEEPPECPECEKNETEKLEESSVV
                     IPPEPPKS"
     gene            957..2129
                     /locus_tag="PKJ17_07895"
     CDS             957..2129
                     /locus_tag="PKJ17_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD75933.1"
                     /translation="MDLKSLLEARRDEIMGEWVRRILTEVSERYSRRPVEEVRETISE
                     ATEAYFAALTEDDFSKMDRFIEKITAMRLDVGFALSEVQGAFELYRTVLVPIFLTELD
                     TSLLPDALQRLNRCLSHTIRRFSDHFQVQHEQSIREHAGNLERVVGERTRELAESEAK
                     YRMLVEEINDGYFIARKGRLVFANRAFCEMHGYSAEEVIGRPYLDFVAPESRGQLTRI
                     RGRRASTGEAPDQYIYLRLHKDGRKLYSENKVKVITYDGATATAGICRDVTERVTLEK
                     QTLRLVELENERKTIALSTLRQLMATLSHYLLNANAIIGGMARRCVRAASDEERHSSM
                     EAIMEQTGKTEGVIAALRKMAEIRTTRYTPESHTLMIDLTREIEKTLAKTEEETSQ"
BASE COUNT          590 a          623 c          755 g          400 t
ORIGIN      
        1 cgacgaatat cccccgccag ggaaaaaagt ggaggagaaa gaggacgacg agcggtgtgg
       61 cgaagatggc gagagaagcc cccgtgtcat ggatggcgat cgccttgccc cagttcttct
      121 cggtgtagta ctcggtgatg agcgggatgg cggaaggtat gtacatcccg tgggcgaagc
      181 caaggaagaa ggcgaagaaa tagaggagat agaaagtgtg aacggcgggg atgagaaacg
      241 ccagaatgct gagaaggctg agagaaagga cgatggtccg cttgtatccc accctgccgg
      301 agagcagccc ggaaaggatg accgaaagac cgtatcccgc ggaaaggaac gtgaagatgg
      361 cgcttgcctt cgcgtggtgc acgccgaact cgtcctccac gagagggagg atcggggaga
      421 agacgatgcg gatggaaaaa ctgacgaacc agacgaaaag gagaaagagg agaaagacga
      481 gcgcctttcc ccggaattcc tcgaaccgtg ccattaatcc aatgtaaccc gataacagac
      541 aatttcacca gaagtttcac gaaaaatgtg ttccccgccc ggattgtttc cgtaccgggc
      601 atgagggcga ggtctccttg acaacagctg gcccggtgct tatcgatgta cagcacgcaa
      661 acaacacgag gaggtgctaa gatggctgaa gcgacatata tatgcagaag gtgcggcatc
      721 accttttcgg ccatacccgg cgaggaggag ccgccggagt gcccggaatg tgagaagaac
      781 gaaacggaga aacttgagga aagttcggtg gtcattcctc ccgagccccc gaagtcctga
      841 ggagggtcgg taagatgcga agccccgttg ttccgttgct ccatgccgcg atttcgatcg
      901 atgtgatcgc accatgttct tgacggttgc ggggttccgg tataggcttt tctgtaatgg
      961 atctcaagag cctccttgaa gcgcgccggg atgagatcat gggggaatgg gtcaggcgga
     1021 tcctgaccga ggtgagcgag cgctattccc gacgcccggt ggaagaggtc agggagacca
     1081 tatccgaggc aacggaagcc tacttcgcgg cactcacgga agacgatttt tcgaagatgg
     1141 acaggtttat cgagaagatc accgccatgc gcctcgatgt cggcttcgcc ctctccgagg
     1201 tccagggggc tttcgagctc tacaggacgg tcctcgtgcc cattttcctc acggagctcg
     1261 atacgtctct cctgcccgat gcgcttcaaa ggttgaaccg ttgcctttcc cacaccatcc
     1321 ggagattcag cgaccatttt caggttcaac acgagcagtc gatacgggag cacgccggga
     1381 acctggagcg ggtggtcggg gaaaggacac gggaattggc ggaatcggag gcgaaatacc
     1441 ggatgctcgt cgaggagatc aacgacggat acttcatcgc aaggaaagga aggctggtat
     1501 tcgccaacag ggcgttctgc gagatgcacg gctattcggc ggaagaggtc atcggacggc
     1561 catatctcga ttttgtggcc cccgaatcgc ggggccagct gaccaggatc cgggggagac
     1621 gggcgtcgac gggggaagcc ccggaccagt atatatacct gaggctccat aaggacggca
     1681 ggaagctcta ttcggagaac aaggtcaagg tgatcaccta tgacggggca acggcgaccg
     1741 ccgggatctg ccgggacgtc acggagaggg tgacgctcga aaaacagacg ctgcgcctcg
     1801 tcgagctcga gaacgaaaga aagacgatcg ccctgtcgac gctgcgccag ctcatggcca
     1861 cgctgtcgca ctatctcctc aacgccaatg ccataatcgg cggcatggcg aggcgctgcg
     1921 tgcgggccgc ttccgacgag gaaaggcact catcgatgga agcgataatg gaacagacgg
     1981 gcaagacgga aggggtcata gcggccctcc ggaagatggc cgagatcaga accaccaggt
     2041 acacgcccga aagccacacc ctgatgatcg atcttaccag ggagatcgaa aaaacactgg
     2101 caaagaccga ggaagagaca tcgcaatgaa agtgtctttc cttctgcaga ccacgttcga
     2161 tccgcgccaa ggacaccctt ctctcatgcc ggaacgacgg cagggggtca ggtcccgaaa
     2221 agccttgcct gagcggagat cagaactcag gccggcagcc cgctcggtta ctcgcagacg
     2281 tatccatagg tgatccagca gggctcgtaa tcacaggagg ggccttccgc cagggctacc
     2341 agctgagcgc tgtagccggc ggggcagc
//
LOCUS       DAOPOA010000366         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_48858_length_2367_cov_2.413062, whole genome shotgun sequence.
ACCESSION   DAOPOA010000366 DAOPOA010000000
VERSION     DAOPOA010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48858_length_2367_cov_2.413062"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKJ17_07900"
     CDS             complement(<1..284)
                     /locus_tag="PKJ17_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75934.1"
                     /translation="MKTVLRKKNFLSGIPACFLLCIVFLVLGSLPLMAGDSTTLGSPG
                     GTLTGYTSKNVSGGSNYVYYPTPGTYSWTIPDDAASKVWVTLAAAGSGGVG"
     gene            complement(316..873)
                     /locus_tag="PKJ17_07905"
     CDS             complement(316..873)
                     /locus_tag="PKJ17_07905"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOD75935.1"
                     /translation="MDGLRQAILNDVEKMRTADPARYQDFMNVAIWVKRQNRKQHPVL
                     SALVSKAITDHVHDNFKYRSAEHRLNFLSLLMGIMKVESGYNPFAKSYANARGLMQVH
                     YPTWGRYFRSQEDVHHLQKNLIVGTGILNLYLARSGNDLRKALLMYLGAPANKPPEQT
                     KEEYNQYVRNVVHSALRFKKHYTAR"
     gene            complement(1015..2145)
                     /locus_tag="PKJ17_07910"
     CDS             complement(1015..2145)
                     /locus_tag="PKJ17_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75936.1"
                     /translation="MAEIQEGSFERFKTVRYLKSLIANTPNDLNIMIWGPPGIGKSIT
                     IQEYVQEQGLGFLDTRLSQLDSIDLRGFPSKNSEDGYADFVPFKNVLPIPELHGEEGI
                     LFLDEANLASIDVMKAGYQLLTDKKIGGYTLPPGWRIMLAGNNPSDAPGLIRDLPPPL
                     KNRIYHFEVTPPVTEEARQDLFNWYYKHNIHPSIIAFLKMNPSKIHDMQHASKENAWP
                     SPRSWVMASIAITGKGIAKDDGNLDFSKYEEMMFLVGNAVGKGHAIDFSSFAKIYERL
                     PNARKVLTDGIDSVANMPDRKDKAVIFALTTAMVANLRDPELYGDPAVVKNYFDYIKT
                     IDKEFAIKSLKDAGAIPGLWQAISKSAEYKPYAREAVQYVRL"
BASE COUNT          478 a          621 c          676 g          592 t
ORIGIN      
        1 cccacgcctc cactgcctgc ggctgccagg gtcacccaga ccttgctcgc ggcatcgtcg
       61 gggatcgtcc acgagtatgt tcccggcgtg gggtaataga cgtagttgga cccgccggag
      121 acgtttttcg atgtgtaacc ggtcaatgtc ccgccaggtg agcccagtgt ggtcgagtcc
      181 ccggccatga ggggaagtga acctagaacg agaaaaacga tgcacagcag gaagcatgcc
      241 gggataccac tgagaaaatt cttcttacgc aaaacggttt tcatgaaaag atcctccttg
      301 tgtggctttt cagtactacc gcgcggtgta gtgtttcttg aacctcaacg cggaatgcac
      361 cacgttccgg acgtactggt tgtactcttc tttggtctgt tccgggggtt tgttcgcggg
      421 ggctcccagg tacatcaaca aggcctttct gaggtcattc cccgaccgtg caaggtagag
      481 gttgagaata ccggtgccga cgatgaggtt cttctgcagg tggtgcacat cttcctgcga
      541 cctgaagtat cttccccatg tcgggtagtg cacctgcatg agtccccggg cgttggcgta
      601 gctttttgcg aaggggttgt acccgctttc caccttcatg atccccataa ggagagagag
      661 aaagttcaga cgatgctccg ccgaccggta tttgaaattg tcgtgaacat ggtccgttat
      721 ggcttttgaa acaagggctg acagcacggg gtgctgcttc cggttctgtc tcttcaccca
      781 gatggccacg ttcatgaagt cctggtacct ggccggatct gcggtccgca tcttctccac
      841 gtcattgagt atcgcctgcc ttagcccgtc cacggtcgtg ggggatgttt ccccccacga
      901 aatggacggc aaacaaagga ggatcaaaac gaggaggatc aggaatttgc gtatgtcata
      961 gtgtctcata tccaggaaat agcacgccgg catgtcattt ctccgatatg tcctctaaag
     1021 cctcacatac tggaccgctt cccgggcgta aggcttgtac tcggcgctct tggatatcgc
     1081 ctgccacagc ccggggatgg ccccggcgtc cttcagggac ttgatggcga actccttgtc
     1141 gatcgtcttg atgtaatcga aatagttctt aaccaccgcg gggtcgccgt agagctcggg
     1201 atcacggaga ttggcgacca ttgccgtcgt cagggcaaat atgaccgcct tatcctttct
     1261 gtcgggcatg ttcgcaacag aatcgatccc gtctgtgagg accttccggg cattgggaag
     1321 cctctcgtat atcttggcga acgaagagaa atcgatcgcg tggccctttc ccacggcatt
     1381 gccgacgagg aacatcatct cttcgtactt gctgaaatcc aggttgccgt cgtctttagc
     1441 tatccctttc cccgttatgg cgatagacgc catgacccat gaccgtggtg acggccaggc
     1501 attttctttc gacgcgtgct gcatgtcatg gatcttgctc gggttcatct tcaggaaagc
     1561 gatgatgctc ggatgtatgt tgtgcttgta gtaccagttg aagaggtcct gccttgcctc
     1621 ttccgtaacg ggcggcgtca cctcgaagtg gtagatcctg ttcttcaggg ggggagggag
     1681 gtcccgtatg agaccgggag catccgacgg gttgttcccg gcgagcatta tcctccaacc
     1741 cggaggcagc gtgtagccgc cgatcttctt gtccgtcagg agctgatagc cggccttcat
     1801 gacatcgatg gaggccaggt tggcctcgtc gaggaagagg ataccctctt caccgtgcag
     1861 ctcggggatg gggagcacgt tcttgaacgg gacgaagtcc gcgtacccgt cttcgctgtt
     1921 ctttgagggg aagcccctga gatcgatgga atcaagctgc gagagcctgg tgtccagaaa
     1981 accgaggccc tgctcctgga catactcctg gatggtgatg ctcttcccga tgccgggagg
     2041 accccagatc atgatattca ggtcgttcgg tgtatttgcg atgaggctct tgagatacct
     2101 cactgttttg aacctttcaa aggatccttc ctgtatttcc gccatttcat cctccgtatg
     2161 tcgttaatgc gttaattaat gctctacggg gtaaatagca ggaagttaat ttgcgtgatg
     2221 aaaaatgagg tggttggtgc tttattgcgc gaaaaaaact atggcgaggg gtgtcggtcc
     2281 tcgagctgat cgatcttgtc gccctgaagc ctggacatga cacactgcgc aagggtcttg
     2341 aaaagcttga acaatttggc agggttg
//
LOCUS       DAOPOA010000367         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_48897_length_2365_cov_3.254978, whole genome shotgun sequence.
ACCESSION   DAOPOA010000367 DAOPOA010000000
VERSION     DAOPOA010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48897_length_2365_cov_3.254978"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKJ17_07915"
     CDS             <1..370
                     /locus_tag="PKJ17_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75937.1"
                     /translation="KLLAYKRLARVRDEDELREMEGELKDRYGALPSPLVNLLEVISL
                     KSLLTRLKVRKAEKSEGQVILHVTDRTPLDMKRLLALAMDDGKGIKLLPDGRIVIRSG
                     LHEGELMNFIRNILMEIAPL"
     gene            457..1479
                     /locus_tag="PKJ17_07920"
     CDS             457..1479
                     /locus_tag="PKJ17_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOD75938.1"
                     /translation="MLENREGLKRWGMKNVAICLCLALCLFACDKKDDSKVLVTINDD
                     KVTMKEFNKELDRIPMNMKMMVASQSGKKACLDRLVIKKLLLAEAQKANIEKDKDFEN
                     RLAEIKEQLIIESLLKKKLTAGANITEDDLQKYYEKNKEKFRKDAEINTRHILVKSEE
                     QARQVKEKLQAGEDFTELAKKYSIDPNAKVTGGEVGFHPKGTLLPEYEAAAFKLTKIG
                     QTSGIVETQFGYHIIRLEGIKPPAYVPFAEVKDFIKQQLMQEKQKEVLDKYIEELKKS
                     AKITINEDLLKDQAPAGPAKPEETKDAKPEVKDTKPEAKEAPAAKPAEPKTATPPKKE
                     TVPQSK"
     gene            1476..>2365
                     /locus_tag="PKJ17_07925"
     CDS             1476..>2365
                     /locus_tag="PKJ17_07925"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOD75939.1"
                     /translation="MKRIPTLIIAATIMALTGFGAQAEVVDRVIAIVNDDIVTLKDFE
                     AYVKIEKRTKFTSIDEYFRNLQMKDRLEAYVEGLLIKQQAKKMGIVVSNEEVSGFIEG
                     IKKQNLITDAELREQLKKDNVTYEQFREGVRLNVLRTRLLMRVLSTEIKVTEASLKSY
                     YEAHQDLFREEEFHLQQIFISGQHPDIKERAEKAYTQLTTGTPFADVARSLSDDPSAR
                     QGGDIGFVKRSDLMPTLREGLTNIPPGSFTQVIPTQYGLHILKLLEVKQAEVLPYEEV
                     KKVVNDRMVMEESNKRYRDY"
BASE COUNT          674 a          640 c          656 g          395 t
ORIGIN      
        1 aaagctcctc gcgtacaagc gcctcgcccg tgtgcgcgat gaagatgagc tcagggagat
       61 ggagggcgag ctgaaagacc ggtatggcgc ccttccctcc ccgctcgtca atctcctcga
      121 ggtcatttcc ctgaagtccc tgctgacgcg actgaaggtc cgtaaggccg agaagagcga
      181 gggacaggtg attctccatg ttacagaccg gacgcccctc gacatgaaaa ggcttctcgc
      241 ccttgccatg gacgacggca aaggcatcaa gctcctgccc gacgggcgga tcgtcatccg
      301 ttccggcctt catgaggggg aacttatgaa tttcataaga aatatattga tggagatcgc
      361 accgctgtga tataagattt cgttacaggc taattgatcg ccatcgtgtg tttcgggtcg
      421 ccccggtcgg gagcagccgg tgataaaggt cgatatatgc tggaaaatcg agaagggcta
      481 aagaggtggg gaatgaagaa cgtggcaatc tgtctgtgtt tggcactgtg tctctttgcg
      541 tgcgacaaga aggatgacag caaggtgctc gtcaccataa acgacgacaa ggtcacgatg
      601 aaggagttca acaaggaact cgacagaata ccgatgaaca tgaagatgat ggtcgcctcc
      661 cagagcggca agaaggcctg cctcgaccgc ctggtcataa agaagctcct tcttgcggaa
      721 gcccagaagg ccaacatcga aaaggacaag gatttcgaga acaggctggc ggagatcaag
      781 gaacagctta tcatcgaatc gctcctcaag aaaaaactaa cggcgggcgc caatatcacc
      841 gaagacgacc tccaaaagta ctacgagaag aacaaggaga aattccgtaa ggacgcggag
      901 atcaacacgc ggcacatcct cgtgaaatcc gaggaacagg ccagacaggt gaaagagaag
      961 ctccaggccg gtgaggattt tacggaactc gccaagaaat actctatcga ccccaacgcc
     1021 aaagtgacgg gtggcgaggt cggtttccat cccaagggca cccttctgcc cgaatacgag
     1081 gcagcggcat tcaaactgac gaagatcggt cagacgagcg ggatcgtcga gacccagttc
     1141 gggtatcaca taatacgact cgaggggata aagccacccg cctacgtccc cttcgccgag
     1201 gtaaaggatt tcatcaagca gcaactcatg caggagaaac aaaaggaagt cctcgacaaa
     1261 tacatcgagg aactgaagaa aagcgcgaag atcaccatca acgaggacct cttgaaggac
     1321 caggctcccg ccggtccggc aaaacccgaa gagaccaagg acgcgaaacc ggaagtgaaa
     1381 gatacgaagc ctgaagcgaa ggaagcccca gcagctaagc cggcggaacc aaagaccgcg
     1441 acaccgccga aaaaggagac ggtaccgcaa agcaaatgaa acgtatacca acactcataa
     1501 tcgccgcaac catcatggcc ctgaccggct tcggcgcgca ggccgaggtc gtcgacaggg
     1561 tcatcgccat cgtcaacgac gacatcgtca cgctgaagga ttttgaggcc tacgtaaaga
     1621 tcgagaagcg gacgaagttc acatctatcg atgaatactt caggaacctc cagatgaagg
     1681 acaggctcga ggcttacgtc gaaggcctcc tcatcaagca gcaggccaag aagatgggca
     1741 tcgtcgtctc gaacgaggaa gtctctggtt tcatagaagg gattaagaaa cagaacctca
     1801 tcaccgacgc cgagctcagg gagcagctca agaaggacaa cgtaacgtac gagcaattcc
     1861 gggagggtgt caggttgaac gtcctccgga cgagactcct aatgcgtgtc ctctccaccg
     1921 agatcaaggt gaccgaggcg agcctgaaga gctactacga agctcaccag gaccttttca
     1981 gggaggaaga gttccatctt cagcagatat tcatctccgg ccagcatccg gacatcaagg
     2041 agagggctga aaaagcctat acccagctca ccacggggac gcccttcgct gatgtggcgc
     2101 ggagcctctc cgacgacccc tccgccaggc agggagggga catcggcttt gtgaagagaa
     2161 gcgatctcat gcccaccctg agggaaggtt tgacgaacat tcccccggga tccttcacgc
     2221 aggtcatccc cacacagtac ggcctccaca tactcaagct cctcgaagtg aaacaggcag
     2281 aggttcttcc ctacgaggag gtcaagaagg tcgtcaacga ccgcatggtc atggaggaat
     2341 cgaacaaacg ctaccgcgac tacat
//
LOCUS       DAOPOA010000368         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_49346_length_2349_cov_3.639058, whole genome shotgun sequence.
ACCESSION   DAOPOA010000368 DAOPOA010000000
VERSION     DAOPOA010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49346_length_2349_cov_3.639058"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..557
                     /locus_tag="PKJ17_07930"
     CDS             63..557
                     /locus_tag="PKJ17_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOD75940.1"
                     /translation="MAPGGESAWSLVLKFFNFFVLVGLLVYFVGKPLKAYLSKRHDTV
                     KTRLEETEKVLKDAQAIRAQYQERLDKLDGEIEVFKKQTMQEAEAEKKKIIEEAHAFA
                     ERIREQARLTAEQENKDMARRIKEEISRLTMEKAEQLITEKITQSDHDKLVEEFIVKL
                     RSMN"
     gene            554..1096
                     /gene="atpH"
                     /locus_tag="PKJ17_07935"
     CDS             554..1096
                     /gene="atpH"
                     /locus_tag="PKJ17_07935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOD75941.1"
                     /translation="MISQSIAKRYAKGLFNVGEKNGKYKEYQAEIEGVLSVLDKEERL
                     KRAIVLPLVEVEKRKEVLSDVMKSLKISSPLASMFTMLLEKNRMGYLNLIRDVYNDLV
                     DEKEGRVKGTLWTAFPVSDEIKVKIEKELGTRLSKKVTLAVQEDKNLIGGVKVAIKGT
                     IIDGSVKRQLHTLQENILKE"
     gene            1102..>2349
                     /gene="atpA"
                     /locus_tag="PKJ17_07940"
     CDS             1102..>2349
                     /gene="atpA"
                     /locus_tag="PKJ17_07940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOD75942.1"
                     /translation="MEIRADEISRIIEQKISGFEKEIDLQETGVIMTVGDGIARIYGL
                     ENAMAGELLDLPHGITGMVQNLEEDNIGAVIFGEDYKIKEGDLAKRTGKIAQVPVGEA
                     LVGRVVDALGNPIDGKGPIEAKEFRPVEQIAPGVVVRQPVKEPLQTGIKEVDALIPIG
                     RGQRELIIGDRGTGKTVVALDTIINQKGKGVFCIYVAIGQKRFSVARIVDLLTEHGAM
                     EYTTIVAATASDSAPLQFLAPFAGTAMGEYFRDSGRHALIVYDDLSKHAVAYRQLALL
                     LRRPPGREAYPGDIFYLHSRLLERSAKWDDAHGGGSLTSLPIIETQAGDVSAYIPTNV
                     ISITDGQIYLEPELFYAGVRPAINVGISVSRVGGNAQIKAMKQVAGKLRLEMAQYREM
                     AAFAKFGSDLDKATQAQLNRGMRM"
BASE COUNT          643 a          596 c          662 g          448 t
ORIGIN      
        1 atggccgatg ttagcaaact ttcgatggaa gcggcacaga agatcttgag aaggagcatc
       61 tgatggcacc cggtggagaa tcagcttgga gtttagtcct aaagtttttt aatttctttg
      121 ttcttgtcgg attgcttgtc tatttcgtgg gcaaaccgtt aaaagcgtac ctctcaaaac
      181 gtcatgatac cgtgaaaacc cggctcgaag agaccgaaaa ggtcctgaaa gatgctcagg
      241 ctatacgggc gcagtaccag gaaaggctgg acaagcttga cggagagatc gaggtcttca
      301 agaaacagac catgcaggaa gcagaggccg aaaagaagaa gataatcgaa gaggcccatg
      361 ccttcgccga aaggatacgg gagcaggccc gtcttaccgc cgaacaggag aataaggaca
      421 tggcccgcag gatcaaggaa gagatctccc gccttacaat ggaaaaggca gagcagttga
      481 taactgagaa gataacccag tcggatcacg ataaactggt tgaggaattc attgtcaaac
      541 tgaggagcat gaattgataa gccaatccat cgccaaaagg tacgcaaagg gattattcaa
      601 tgttggtgag aagaacggga aatacaagga ataccaggcg gagatcgagg gtgttctgag
      661 tgtccttgac aaggaagagc gcctgaaaag ggcgatcgta cttcctctcg tcgaagtgga
      721 gaaacgcaag gaagtcttaa gtgatgtcat gaagtctctc aagatctcct ctccacttgc
      781 cagcatgttt accatgcttt tagagaagaa caggatgggt tatttaaacc tcatccggga
      841 cgtctacaat gatctcgtcg atgaaaaaga aggaagggtc aaagggactc tctggaccgc
      901 atttcctgtc agcgacgaga tcaaggtaaa aatagagaaa gaattgggaa caagacttag
      961 caagaaggtc actctcgcgg tacaggaaga caagaacctt ataggaggcg tcaaggtggc
     1021 catcaaaggg accatcattg acggtagcgt caaaagacag ctgcatacac tgcaggaaaa
     1081 tatactgaag gagtagtgtc tatggagatc agggccgacg aaataagcag gattatagag
     1141 cagaagatat cgggcttcga gaaggagatc gaccttcagg aaaccggtgt catcatgacg
     1201 gtaggcgatg gtatcgcaag gatctacgga cttgagaatg ccatggccgg cgaactcctc
     1261 gatctgcccc acggcatcac gggcatggtg cagaaccttg aggaagacaa catcggtgcc
     1321 gttatcttcg gcgaagacta taagatcaag gaaggcgact tggccaagag gaccgggaag
     1381 atcgcccagg tccccgtcgg tgaggccttg gtcggcaggg tcgtggacgc cctcggaaat
     1441 cccatagacg gcaaaggccc catcgaagcg aaggaattcc gtcccgtcga gcagatcgcc
     1501 cccggcgtcg ttgtccgcca gcccgttaaa gagccgcttc agactggcat caaagaagtc
     1561 gatgcgctga tcccgatcgg aagaggccag agggaactca tcatcggcga tcgcggcacc
     1621 ggcaagacag tcgtcgccct cgacaccatc atcaaccaga aaggcaaagg cgttttctgt
     1681 atatatgtcg ctatcggaca gaagaggttc tccgtggcgc gtatcgtcga cctcctgaca
     1741 gagcacggcg caatggagta cacgacgatc gttgcagcga ccgcgagcga ctccgcccct
     1801 ctgcagttcc tggcaccctt tgcaggtacg gccatgggcg aatacttcag ggacagcggg
     1861 aggcacgccc tcatcgttta cgatgacctg tcaaagcatg ccgttgcata ccgccagttg
     1921 gccctgctcc tcaggcgccc cccgggacgc gaggcatatc cgggcgacat cttctacctg
     1981 cactcacggc ttctcgaaag gtcggcgaaa tgggacgatg cccatggcgg cggctccctg
     2041 acatcacttc ccatcatcga aacacaggct ggcgacgtct ccgcttacat tcccacgaat
     2101 gtcatctcga tcaccgatgg ccagatatat ctcgaaccgg aattgttcta cgccggtgtc
     2161 cgtcctgcca tcaacgtcgg tatatccgta tcccgtgttg gcggcaacgc gcagatcaag
     2221 gcaatgaagc aggtcgccgg caaacttagg ctggagatgg cccagtaccg tgaaatggcc
     2281 gcattcgcca agttcggcag cgacctcgac aaggccacgc aggcccagct caaccgcggc
     2341 atgcgcatg
//
LOCUS       DAOPOA010000369         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_49347_length_2349_cov_3.585004, whole genome shotgun sequence.
ACCESSION   DAOPOA010000369 DAOPOA010000000
VERSION     DAOPOA010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49347_length_2349_cov_3.585004"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="PKJ17_07945"
     CDS             <1..1585
                     /locus_tag="PKJ17_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOD75943.1"
                     /translation="HLSRPVSPWTGIAWELIEEGPDTCGTYPSGFTPAPGGKRAANQD
                     NVRILIAVLADDVDEVKDGLSRLSFIETELPDFSGTISDRVRTLQEDLLQLSATIEET
                     VTEVRALASDRRTLITLKAFWTAARNERLALTKTLGGRWVHLLTGYIRARDVERLQGA
                     FAKEFPDSVIILDDPSEDENVPVSLSLPKVFRPLSLLVEMFGLPPYRGFDPTPYLHVN
                     FYLFFGICFSDVGYGLMLIATSLYLIKRTKQYQGVADFGRILLGGGISTVIFGALLGS
                     WFGDLYMEKYLGENNLLMRIQSFFVVLDPISKTIYALLFALLIGILNQFFGIALRMYG
                     TIRKGDWKSAVFDGFFWYLTLPGLLILVSKLFVETPQTLYRTGLALFGAGALGLILTQ
                     GRDMKNPAGRILGGIVSLYGIVGSYGITAFIGDVLSYCRLLALGLTTSIVGTTFNMLG
                     GLLRDIPYVGLFCFILVVVAGHLFNFVISLLGAFVHSMRLIFVEFFGRFYDAGARPFR
                     PLGFDSPLCEMKKEARKNG"
     gene            1613..2128
                     /locus_tag="PKJ17_07950"
     CDS             1613..2128
                     /locus_tag="PKJ17_07950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF005124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOD75944.1"
                     /translation="MTPEWIEIGRGLCYAGAGLTFGLAGAGSACGIAIASHAGAGVMR
                     EKPELFGRMLIMIALPGTQGFYGFIAAILIMTQTGLIGGGNLKISLGTGLALFFIGLG
                     NGIAQLVSSIKQGEASAAGIALIARRPESAGRAILFPAFVETYAVVALLATILFIIFL
                     TQPSVLQLLPQ"
     gene            2153..>2349
                     /locus_tag="PKJ17_07955"
     CDS             2153..>2349
                     /locus_tag="PKJ17_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75945.1"
                     /translation="MSLDKIKEAVLKASRTEAEHISAAAARQAKEKLEIQKENLRREF
                     EYQYEARSRFIEEEYSRKLAH"
BASE COUNT          455 a          724 c          674 g          496 t
ORIGIN      
        1 gcacctctca aggccggtat ctccctggac cggcatcgcc tgggaattga tcgaggaggg
       61 gcccgacacc tgcgggacat acccgtccgg gttcacaccc gcccccgggg ggaaaagagc
      121 ggcgaatcag gacaacgtca ggatactcat tgccgtcctc gccgatgacg tggacgaggt
      181 gaaggacgga ctgtcccggc tttccttcat cgaaacggag ctccccgact tctcggggac
      241 gataagcgac cgtgtcagga ccctccagga agacctgctc cagttgtccg ccaccatcga
      301 ggagaccgtc acggaggtca gggcgcttgc ctccgaccga cgtaccctca ttaccctcaa
      361 ggctttctgg acggctgcca gaaacgaacg actggcgttg acaaagaccc tcggcgggag
      421 atgggtccat cttctcaccg ggtacatacg ggcccgggat gtcgagcgtc tgcaaggcgc
      481 cttcgccaaa gagtttcccg actctgttat catcctcgac gacccttccg aagacgagaa
      541 cgtaccggtt agcctctccc ttccgaaggt cttccggccg ctctccctgc tggtggagat
      601 gttcggcctg ccgccgtacc gcggctttga ccccacgccg tatctgcacg tgaacttcta
      661 tctcttcttc ggcatatgct tttccgatgt cgggtatggc ctcatgctca tcgcgacctc
      721 actctatctc attaaaagga caaaacaata ccagggcgtg gcggatttcg ggcgtatcct
      781 tctcggcggc ggcataagta ccgtcatctt cggggccctg ctggggtcct ggttcggcga
      841 cctttacatg gaaaagtacc tgggcgagaa caacctgctc atgcgcatac agtccttctt
      901 cgtggttctc gaccccataa gcaagacgat ctacgccctg ctcttcgccc tgctgatcgg
      961 cattctgaac cagttcttcg ggatcgccct caggatgtac gggaccatca gaaagggaga
     1021 ctggaagagc gccgtctttg acgggttctt ctggtacctg acactccccg gcttgcttat
     1081 actcgtcagc aagcttttcg tcgaaacgcc gcagacactc tatcggaccg gcctcgcgct
     1141 gttcggcgcc ggggcgctgg gccttatcct gacccagggg cgggacatga agaaccccgc
     1201 gggccgtatc ctgggcggga tcgtcagcct ttacgggatt gtgggaagct acgggatcac
     1261 cgccttcatc ggcgacgtcc tctcctattg ccgcctgctt gccctcggtc tcaccacgtc
     1321 catcgtggga acgaccttca acatgctcgg cggacttttg agggacattc cttacgtggg
     1381 actcttctgc tttatccttg tcgtcgtcgc gggccatctt ttcaacttcg tcatcagtct
     1441 cctgggcgcc ttcgtccact cgatgcgtct catattcgtc gagttcttcg gacggttcta
     1501 tgacgccggg gcgcggccct tccggccgct cgggttcgac tcgcccctgt gcgagatgaa
     1561 aaaggaagca agaaaaaatg gatgatcaca aaaactttgg aggaagaata caatgacccc
     1621 agaatggatc gagattggtc gtggtctttg ctacgcgggg gcgggcctca cctttggtct
     1681 ggcgggcgcc ggttcggcct gcgggatcgc catcgcctcc catgccggag cgggagtgat
     1741 gcgtgaaaaa ccggagcttt tcgggcgcat gctcatcatg atagccttgc cgggcacgca
     1801 gggattctac ggcttcatcg ccgccatcct catcatgacc cagacgggcc tcatcggggg
     1861 gggaaatctc aagatatccc ttggaacggg cctcgcgctc ttcttcatcg gcctcggcaa
     1921 cgggatcgcc cagctcgtct cttccataaa gcagggagag gcatcggcag cgggaatagc
     1981 gctcatcgcg agacgaccgg agtcggcggg acgggccatc ctctttcccg cctttgtcga
     2041 gacctacgcc gtcgtggccc tccttgcgac catcctcttc ataatcttcc tcacgcaacc
     2101 ctctgtgctg cagctcttgc cacagtagac ggacgaagga gccgggcggg acatgagtct
     2161 tgacaagatc aaagaagcgg tgttaaaggc atcaaggacg gaggcggagc acatcagtgc
     2221 cgccgctgcg agacaggcga aagaaaagct ggagatccag aaggagaacc tgaggcggga
     2281 attcgagtac cagtatgagg cccgttcccg cttcatcgag gaggagtatt cccggaaact
     2341 ggcccactt
//
LOCUS       DAOPOA010000370         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_49362_length_2349_cov_2.285527, whole genome shotgun sequence.
ACCESSION   DAOPOA010000370 DAOPOA010000000
VERSION     DAOPOA010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49362_length_2349_cov_2.285527"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKJ17_07960"
     CDS             <1..442
                     /locus_tag="PKJ17_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD75946.1"
                     /translation="FRDIAKGVYLTGEIPRLSSFERGDKRLAVKRGEVYDVDTVPDDG
                     ALVISTEMGLVVLLGCAHAGMVNTLEHVRRNLPGAPLHAVIGGTHWGILPEDAVELSI
                     KAIADMEVGTVGVCHCTGIAYAAKLIRAVGERGFYASVGTVFEV"
     gene            552..1307
                     /locus_tag="PKJ17_07965"
     CDS             552..1307
                     /locus_tag="PKJ17_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOD75947.1"
                     /translation="MSVVLESLKNDVFTITLNRPDKKNAMDYDLLKGLYEAMKNAARE
                     KAPFVVIRGSSGAFCSGGDIIAFRESEDAEALIDAEAGVLNESIKMIRNIPAIVIAVI
                     EGVAVGAGVGLALACDLSIATKKTIMNMGYRRIGLTPDGGGSIFLPRIIGAKLFNELY
                     LLSRNVTMDEAKELGLVNILCEDAEVDEKLDELIRNLKALPMETMGKFKDLVNRCLFF
                     GLETHLDMERRYVADLAGDPLFKQRLDEFFRRK"
     gene            1311..>2349
                     /locus_tag="PKJ17_07970"
     CDS             1311..>2349
                     /locus_tag="PKJ17_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75948.1"
                     /translation="MKGLYLFAIAAYICVLWLTGAFVTRKSRSADAYLVASRGLSVPF
                     VAVLIAGTWIGGVSIVGMAQGSFIHGLSALWFQAGIWIAMFVTAMILPRILSGRKTYS
                     ILDVVGGLYDRRTTRLAGLLQLVFSIWIVTMQIVGGGAILSVLTKGTISFNGGMVLTT
                     VVFTLYNVMGGFVATAYTNLIHIVALVFGIFLGGFYVIFSTGSLQTMAGQGGHYYTLF
                     GDLGVFQALSWAFINFTLGVLAQPVINTASSARTFTEGRKGIVIGNLIAIPVVIMAAL
                     CGIIAKQRFPEINSLAALPSLIAIVPPWIAVFFLISMWAPLMSSGSPFLMGATTLAVK
                     GYVAPLFGMESD"
BASE COUNT          445 a          664 c          703 g          537 t
ORIGIN      
        1 tttccgcgac atcgcgaagg gggtgtacct caccggcgag ataccgcggc tttcgtcctt
       61 cgaaaggggc gacaagcgcc tcgcggtaaa aaggggagag gtctacgatg tcgataccgt
      121 gcctgacgac ggagccctgg tcatatccac ggaaatggga ctggtcgtcc ttctgggatg
      181 cgcccacgcg gggatggtga acaccctcga acatgtccgc aggaatcttc ccggtgcgcc
      241 tctccacgcc gtcatcggcg gtacccactg gggcatactc cccgaggatg ccgtggaact
      301 ctcgataaag gccatcgcgg atatggaggt cggcacggtc ggtgtttgtc actgcaccgg
      361 catagcgtac gcggcaaaat tgatacgggc agtgggcgaa cggggtttct atgcctcggt
      421 ggggaccgtc ttcgaggtgt gaccgtcggc atcgggaacg gatctttcgc ctcatcattt
      481 ttgccctttt acggataacg gtgaattgag cgttatcgat gcgggttaga cacacaatca
      541 aggaggaatg tatgtcggtt gttttggagt cactgaagaa cgatgtgttc actatcacgt
      601 tgaacaggcc ggacaagaag aacgccatgg attacgacct tctcaagggg ctttacgagg
      661 cgatgaagaa cgccgcgcgg gaaaaagccc cttttgtcgt catccggggt tcgtcagggg
      721 cattctgctc cggtggggat ataatagcct tcagggagtc cgaggacgcc gaggcgctca
      781 tcgacgccga ggcgggtgtc ctcaacgaga gcataaaaat gatccggaac atacctgcca
      841 tcgtcatagc cgtcatcgag ggtgtggccg tcggtgccgg tgtgggactg gcccttgcct
      901 gtgacctctc gatcgccacg aagaagacca tcatgaacat ggggtatcgc aggataggtc
      961 tcacccctga cgggggaggc agcatctttc tgcctcgtat cataggggcg aagcttttca
     1021 atgagctcta cctcctgtcc cggaatgtga ccatggatga ggcgaaggaa ctggggcttg
     1081 tcaatatact gtgtgaagac gcggaagtgg acgagaagct cgacgagctc atccgaaacc
     1141 tgaaggcgct ccccatggag actatgggca agttcaagga cctcgtgaac cgctgtctct
     1201 tcttcggcct ggagacacac ctcgacatgg aaaggcgcta tgtggcggat cttgccggtg
     1261 acccgctctt caaacagagg ctcgatgagt tcttcaggag aaaatagagc gtgaagggcc
     1321 tctacctttt cgcgatagcg gcctacatct gtgttctctg gcttaccggg gccttcgtta
     1381 cacggaagtc ccggtcggcc gacgcttacc ttgtcgcctc gcgggggctg tcggtgccct
     1441 tcgtagccgt cctcatcgcc ggtacatgga tagggggcgt ctccatcgtg ggcatggccc
     1501 agggttcctt catccacggc ctgtccgccc tgtggttcca ggccggtatc tggatagcca
     1561 tgttcgtcac cgccatgatc ctccccagga tcctttcggg caggaagacc tattccattc
     1621 tcgacgtcgt gggaggtctc tacgacagga ggacgacgcg ccttgccggt ttgctccagc
     1681 ttgttttttc catctggatc gtgactatgc agatcgtggg cggcggtgcc atcctgtccg
     1741 ttcttacgaa gggaaccatt tctttcaacg gcggcatggt cctcaccact gtcgtcttca
     1801 cgctttacaa cgtgatgggc ggcttcgtcg ccacagcgta tacgaacctc atccatatcg
     1861 ttgccctcgt gttcggcatc tttctgggcg gtttttacgt catcttctcg acgggcagtt
     1921 tgcagaccat ggccgggcag ggtggccact attacaccct tttcggggac ctcggcgttt
     1981 tccaggcgct gagctgggcg ttcatcaatt tcacgctggg ggtgctggcg cagcccgtca
     2041 taaacacagc gtcctcggcg aggaccttca ccgaagggag gaagggaata gtcatcggca
     2101 acctgatcgc catccccgtc gtcatcatgg cggccctttg cgggataata gcgaagcagc
     2161 gctttccgga gattaattcc ctcgctgctc tcccgtcgct cattgccatc gtgccgccct
     2221 ggattgccgt cttctttctc atcagcatgt gggcgcctct catgagctcc ggctcccctt
     2281 ttctgatggg agcgacgacc cttgccgtga agggctacgt ggcgcccctg ttcggcatgg
     2341 agagcgaca
//
LOCUS       DAOPOA010000371         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_49396_length_2347_cov_3.635253, whole genome shotgun sequence.
ACCESSION   DAOPOA010000371 DAOPOA010000000
VERSION     DAOPOA010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49396_length_2347_cov_3.635253"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1153
                     /locus_tag="PKJ17_07975"
     CDS             179..1153
                     /locus_tag="PKJ17_07975"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HOD75949.1"
                     /translation="MRKTAIKILVMGAALALVLFVVFMINQTASLVALGDRIHPLAGD
                     IVLWVLIAFYTLCVIVPVYLFITLPAPLVPPRSEISPEFPRYLQEMAKRLGRNPHIGR
                     TVAASKEDVENALRELERRADDAMKKAAGRIFLATAISQNGKLDGLIVLAAQSRLVLD
                     IARVYYQRPTIRNLIFLYANVAAMVFIAGELEDMDLAAIVQPVLTGILGSAAGAIPGF
                     QVASMMLVSSVISGSSNAFLTLRVGAITKQYCGSLVMPTKRTVRRQATLEATRMLGSI
                     VAEGTKKVYDAIWTSSKSRLGTAIDDIGAYAKRIWMKLGGEGPKSAEP"
     gene            complement(1150..1530)
                     /locus_tag="PKJ17_07980"
     CDS             complement(1150..1530)
                     /locus_tag="PKJ17_07980"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOD75950.1"
                     /translation="MAYTASTASNKDEKAGTVFAGDLEVQGTVKFKSSLMIEGIFSGE
                     IISEGVLTVGPSAKVTATIRTKTLVSRGEINGDVYADEQVVLEETAVHNGNITSPGIV
                     IERGSTFNGSCSMKRADTGNTGNF"
     gene            complement(1717..1992)
                     /locus_tag="PKJ17_07985"
     CDS             complement(1717..1992)
                     /locus_tag="PKJ17_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HOD75951.1"
                     /translation="MANDPGFGYRVTATVVETKGSCNAGHKAGDTFELSCHNPAGLCG
                     FFYHSIFGDLEAFQFGGSLPWWDGDKIEVQCPDVANMVTLELERKKR"
BASE COUNT          495 a          685 c          653 g          514 t
ORIGIN      
        1 agggcgatgg tgatatcagg cacgttcagc ctcaacgacc acgaagagat acgatgggtg
       61 agaccggcgg agctggccga ctacaatttc cccgaggcag acaaacccgt ggtggagaaa
      121 ctggtcagtc ttggcgaaag gcgagatagg tcagaatgac cgaaagacag gggacgtgat
      181 gcgcaagaca gcgatcaaaa tactggtgat gggcgctgcg ctggccctcg ttctcttcgt
      241 cgtcttcatg atcaaccaga cggcttctct cgtcgcgctc ggggaccgca tccatccgct
      301 tgcgggcgac atcgtcctgt gggtcctcat agctttctat accctctgcg tcattgtgcc
      361 cgtgtacctc ttcatcacgt tgccggcgcc ccttgtcccg ccccgtagcg agatctcgcc
      421 cgaatttccg aggtatctcc aggagatggc aaaaaggctc ggacgaaacc ctcacatcgg
      481 gaggaccgtc gcggcctcaa aagaagacgt ggagaacgcc ctgcgggaac tggaaaggag
      541 ggcggacgac gcgatgaaga aggcagccgg acgcatattc ctggccacgg cgatatcgca
      601 gaacggcaaa ctggacggcc ttatcgtgct cgctgcccag tcacggctcg ttctggacat
      661 cgcgcgcgtc tactaccagc gtcccaccat ccgcaatctc atcttcctct acgcgaatgt
      721 cgccgccatg gtcttcattg ccggtgagtt ggaggacatg gacctggcag cgatcgtcca
      781 gccggtccta acgggtatcc tgggttcggc cgcgggggcg atacccggtt ttcaggtggc
      841 cagcatgatg cttgtaagct ccgtcatatc ggggtcgtcc aatgccttcc tcacacttag
      901 ggtgggggcg ataacgaaac agtattgcgg gtctctcgtc atgcccacga agcggacggt
      961 ccgccgccag gccaccctgg aggcgacaag aatgctgggt tccatcgtgg ccgagggaac
     1021 gaagaaggta tacgacgcga tctggacctc gtcgaagagc aggttaggga cagctatcga
     1081 cgatataggc gcctacgcga agaggatctg gatgaaactg ggcggcgagg gaccgaagag
     1141 cgccgaaccc tagaagttgc ccgtgttccc cgtgtccgcc ctcttcatgg aacaggaccc
     1201 gttgaaggtc gaccctcttt ctatgacgat gccgggagag gttatattgc cgttgtggac
     1261 ggccgtctct tcaagcacga cctgctcgtc ggcgtaaacg tctccgttga tctcgccgcg
     1321 ggacacgagg gtctttgtcc gtatcgtggc ggtgacctta gcggatggcc ccaccgtcag
     1381 gacgccttcg gagatgatct cgcccgagaa gatgccttcg atcatgagcg aactcttgaa
     1441 ctttacagtc ccctggacct cgaggtcgcc ggcaaacacc gttccggcct tttcatcctt
     1501 gtttgaagca gttgaagctg tgtacgccat tctttcctct tttagttcac gtattttcta
     1561 ctctatttcc ccctgctgga cgggaaggtt gtacattgtg agcatatatc ttaccagatg
     1621 ttgccggaaa acgcgaaatt aaatctccac caccctgaag acggttcaag agttcaaggg
     1681 ttcaaaggct ttccctcttg aacccttgaa ccgtctttat ctctttttcc tttccagttc
     1741 cagcgtgacc atgttcgcca cgtcggggca ttggacctct attttgtcgc cgtcccacca
     1801 ggggaggctg ccgccgaact ggaatgcctc gaggtcgccg aagatgctgt gataaaaaaa
     1861 gccgcacaac cccgcgggat tgtgacagct caactcaaag gtatcacccg ctttgtgacc
     1921 ggcattgcag cttcccttcg tctcaacgac cgtcgccgtt accctgtacc cgaagccagg
     1981 atcatttgcc atgtgaattg acctcctttc tcatagtatg acctgcccca ttccatcgga
     2041 acgccttatt acctatcata cagatattcc ggaaatacgc aacggttggt gagcgcccgc
     2101 ggaaagagac gttccgccaa aggggatgac gaccgatcac caacggagaa gataatgacc
     2161 attccggggt cctcaggtcc cggcccttat tgagatgatg tccccatcct caacgatgta
     2221 cgtcttgcct tcgagccgcc agacgccggc cttcttgcat tcagccatac cgccgtactt
     2281 catgagatcg gcgtaggaga cggtctcggc acggatgaac ttcgaggcca ggtcggtatg
     2341 gatggcc
//
LOCUS       DAOPOA010000372         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_49425_length_2346_cov_3.847665, whole genome shotgun sequence.
ACCESSION   DAOPOA010000372 DAOPOA010000000
VERSION     DAOPOA010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49425_length_2346_cov_3.847665"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PKJ17_07990"
     CDS             <1..233
                     /locus_tag="PKJ17_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75952.1"
                     /translation="RVIVEGLQKAPPGAVVNTVPFKPAAKPGDSAQVPANASPPAKTS
                     DTAPAKTSAPAEMNPPPKTESIKPAEQPAKAK"
     gene            247..>2346
                     /locus_tag="PKJ17_07995"
     CDS             247..>2346
                     /locus_tag="PKJ17_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD75953.1"
                     /translation="MLSKFFLDRPVFAWVIAIIIMLVGVLAIYNLPISQYPPIAPPSI
                     YIASSYPGASAETVENSVTQIIEQKLTGLENMIYLSSTSDSSGSSRITLTFSPGTDPD
                     MAWSKVQNKLQLALTSLPEAVQRQGVTVGKATRNWLMIIGLISEDASMDGNDLRDYAQ
                     SNLEKVIARIPGVGEVENFGSQYAMRIWFDPTKLVNYNLTVANVTEALTVYNVEVSAG
                     QFGGTPAVPGQRLNTSIIVQSMLKTPEEFASIPVRTNPDGSVVHIRDIGRVELGTDVY
                     DIEAFFNGKPSAGMGIRMASGANALDTADAIKAKLADMSRFFPKGLKVVYPYDTTPFV
                     RVAIGEVVETLLEAILLVFLIMWLFMGSIRATLIPTIAVPIVILGTFAVLEFFGFSIN
                     MLTMFAMVLSIGLLVDDAIVVVENVERIMSEEGLPPKEATAKSMKQITSALIGIGLVL
                     SAVFGPMAFFGGSTGVIYRQFSVTIISSMLLSVLVALVLTPVLCATILKPVPKGHEPA
                     ENALPILRPFFSWFDRKFFSLRDRYVSIVGKSFRRRKWYFVGYFVIAGLAAMTLLHMP
                     TSYVPDEDQGILLAQVMMPTGSTIEQTKEVVKKLETYFQEKEKDAVKSCMTISGVGFS
                     GRTQTNGMVFVQLKDWKERGKSSLKVKAVAQRASMAFSQYRNALVFAFPPPSVIELGQ
                     ATGFDFMLVDRGGLGHQK"
BASE COUNT          533 a          695 c          638 g          480 t
ORIGIN      
        1 acagggtaat agtcgagggc cttcagaagg cgcctccggg agcggtcgtc aacactgtgc
       61 cttttaagcc ggcagcaaaa ccgggtgaca gtgcacaggt cccggcgaat gcctcacctc
      121 ccgcgaagac gagcgacacg gccccggcga aaacgagcgc cccggctgag atgaatcccc
      181 cgccgaaaac cgaaagtata aaaccggcgg aacagccagc taaagcaaag tgagcggagg
      241 caattaatgt tatcgaaatt ctttctcgac cgccctgtct tcgcatgggt catcgcgata
      301 atcataatgc tcgtcggggt tctggccatc tacaatctgc cgatctccca gtatcccccg
      361 atcgcacccc catcgatcta catagccagc agctatccgg gcgcctccgc cgaaacggtc
      421 gagaacagcg tcactcagat aatcgagcaa aagctcaccg gcctcgagaa catgatctac
      481 ctgtcctcca ccagtgattc gtcaggcagt tcgcgcatca cgctgacatt ttccccgggc
      541 acggaccccg acatggcatg gtcgaaggtc cagaacaagc tccaactcgc cctgacaagc
      601 ctgcccgagg ccgtgcagcg ccagggagtt acggtcggca aggcgacgag gaactggctg
      661 atgatcatcg gcctcatctc cgaggacgca agcatggacg gcaatgacct gagggattat
      721 gcccagtcga acctcgaaaa ggtgatcgcg cgcatccccg gggtcggcga ggtggagaat
      781 ttcgggtccc agtatgcaat gcggatctgg ttcgacccga ccaagctcgt gaattacaac
      841 ctcaccgtgg ccaacgtgac cgaagcgctc acggtgtaca acgtggaggt atccgcgggg
      901 caattcggcg gaaccccggc agtcccgggc cagagactca acacgtccat cattgtccag
      961 agcatgttga aaacccccga agaattcgca tcgataccgg tccgcaccaa tcccgatggt
     1021 tccgtggtcc atatcaggga tatcgggcgc gtcgagctcg gtaccgacgt gtatgacatc
     1081 gaagccttct ttaacggaaa gccctccgca gggatgggta tacgcatggc atccggcgcc
     1141 aacgccctgg atacagcgga cgctatcaag gcaaagctcg ccgatatgag ccgattcttc
     1201 cccaagggcc tcaaggttgt ctatccctat gacacaacgc cttttgtcag ggtagcgatc
     1261 ggcgaggtcg tcgaaaccct tctcgaggcc atcctgctcg tgtttctcat catgtggctt
     1321 ttcatgggaa gcatacgggc taccctgatc ccgaccatcg cagtgcccat cgttatcctg
     1381 ggaactttcg cggtgctgga gttcttcggt ttctcgataa acatgctgac gatgttcgcg
     1441 atggtcctat ccataggact tctcgtcgat gacgcgatcg tcgtcgtaga gaacgtcgaa
     1501 cgcatcatga gtgaggaagg gcttcctccc aaggaagcca cggccaaatc catgaagcag
     1561 atcacaagcg cgctcatcgg catcggactc gtactgtcgg ccgttttcgg gccgatggct
     1621 tttttcggag gttctacggg cgttatttac cgccagttct cggtcaccat catctcatcg
     1681 atgctccttt ccgttcttgt cgcactcgta ctgacacccg tactgtgcgc taccatcctg
     1741 aagccggtgc cgaaaggcca cgaaccggcc gagaacgccc tgcctatcct gcgcccgttc
     1801 ttcagctggt ttgacaggaa atttttcagc cttcgtgaca gatacgtcag tattgtcgga
     1861 aaatccttcc gaagacggaa atggtatttc gtcggatact tcgtcattgc cggactcgcc
     1921 gcgatgaccc tcctccacat gccgacatcg tatgtccccg atgaggacca ggggatactg
     1981 cttgcacagg ttatgatgcc gaccggttcc accatcgagc aaaccaagga agttgttaaa
     2041 aaactggaga cgtatttcca ggaaaaagag aaggacgcgg tgaagtcatg catgaccatc
     2101 tccggcgtgg gattctcggg acggacccag acgaacggca tggttttcgt acagttaaaa
     2161 gattggaagg agcgtggcaa gagcagcctc aaggtgaaag ccgtcgccca gcgtgcaagc
     2221 atggcattct cacagtaccg caatgccttg gtattcgcat tccctccgcc ttctgtcatc
     2281 gaactcgggc aggcgaccgg gttcgatttc atgctcgtcg accggggtgg tctggggcac
     2341 cagaaa
//
LOCUS       DAOPOA010000373         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_49645_length_2339_cov_2.824431, whole genome shotgun sequence.
ACCESSION   DAOPOA010000373 DAOPOA010000000
VERSION     DAOPOA010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49645_length_2339_cov_2.824431"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /gene="lptC"
                     /locus_tag="PKJ17_08000"
     CDS             complement(<1..504)
                     /gene="lptC"
                     /locus_tag="PKJ17_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOD75954.1"
                     /translation="MSNRKIIIFVSFGCIAASLLLALYFFVSKPERQPVPVAEESNKA
                     VVFKNVKYTGERRGVVDWELTAKVARKYIDRPLIELEDLKGLYKPKPDVSIEFTGTKG
                     SMDTEKESGRVDNVEVFYKGEYTLKSPYMDFDFKNGVTTTKDVVDIKGEKVDMRGTGL
                     LANTNEET"
     gene            complement(522..1490)
                     /locus_tag="PKJ17_08005"
     CDS             complement(522..1490)
                     /locus_tag="PKJ17_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOD75955.1"
                     /translation="MDHVRTGKDILRKEAEALLKVMEGLDDSFNRAAETIFGCTGRVV
                     LTGMGKSGLVGKKIAATLSSTGTPALFVHPADSLHGDLGMLQRGDVLIVISNSGETEE
                     VLSIIPWVKRLDIPLVAITGDAASTIARLGDVALVVNVEEACPYNVVPTSSTTATLAL
                     GDALAIAVMERRAFNVEDFASLHPGGSLGRKLFLRVEDLMHTGEAVPGVSAETSMKDA
                     ILEITSKRLGVTGVYDGEGRLAGIITDGDLRRAIERYDDSLLAKSAGEVMTPGPKVVR
                     RDALAAYALKKMEEFSITSLFVVNDEDSARPVGIIHIHDLLRAKVV"
     gene            complement(1478..2299)
                     /gene="kdsA"
                     /locus_tag="PKJ17_08010"
     CDS             complement(1478..2299)
                     /gene="kdsA"
                     /locus_tag="PKJ17_08010"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOD75956.1"
                     /translation="MQKEITIGNVTVGRRPFVFIGGPCVIEGRDISLRTAESIARITA
                     GLGIPFIFKSSYDKANRTSISGFRGPGLDEGLAILQQVKGEVGVPVLTDVHSAGEAAA
                     AAEVADVLQVPALLSRQTDILTACGRTGRVVNIKKGQFLSPGDMAHAIRKVESTGNRS
                     ILVTERGTSFGYHTLVNDFRAIPIMRDFGYPVVFDATHSVQQPSAMTDRSGGEARFIV
                     PLARAAVAVGVEGVFMEVHPDPARALCDGDNSLPLDELEGVLRTLRRIEEALWTT"
BASE COUNT          380 a          748 c          714 g          497 t
ORIGIN      
        1 ggtctcctcg ttggtgttgg cgagaagccc cgtgccgcgc atgtcgacct tctctccctt
       61 gatgtccacc acatcctttg tcgtcgtgac gccgttcttg aaatcgaagt ccatgtaggg
      121 gctcttcagg gtgtactctc ccttgtagaa gacctccacg ttgtcgaccc tgcccgactc
      181 cttctcggtg tccatggagc ctttcgtgcc ggtgaactca atggagacgt ccggtttcgg
      241 cttgtacagc cccttcaggt cttcgagctc gatgaggggc ctgtctatgt acttccgggc
      301 gaccttcgcc gtgagctccc aatccaccac gccgcggcgt tcacccgtgt acttcacgtt
      361 cttgaagacg acggccttgt tgctttcctc cgctacgggg accggttgtc tttcaggctt
      421 gctcacgaag aaatagaggg caagcaggag gcttgccgcg atgcagccga aagagacgaa
      481 gattatgatc ttcctgttgc tcatgtgtcc tcatatgcca ttcacaccac cttggccctc
      541 aagaggtcgt ggatgtgtat gatgcccacg ggccgcgcgc tgtcctcgtc gttcacgacg
      601 aacagggatg tgatcgagaa ttcctccatt ttcttcagcg cgtaggcggc gagggcatcc
      661 ctgcggacga ccttgggccc cggggtcatg acctcacccg cactcttcgc caggagagaa
      721 tcgtcgtagc gttcgatcgc gcgcctgaga tctccgtccg ttatgatccc cgcgaggcgc
      781 ccttcgccgt cgtagacccc cgtgacgcca agcctcttcg acgttatctc gaggatggcg
      841 tccttcatgg acgtctcagc ggagactccg gggacggcct cgccggtgtg catgaggtcc
      901 tccactctga ggaagagctt cctgcccaga gagccgccgg gatggaggga cgcgaagtct
      961 tccacgttga aggcccgcct ttccatgacg gcaatggcga gggcgtcgcc gagcgcgagc
     1021 gtcgccgtgg tgctcgacgt gggcaccacg ttgtagggac aggcctcctc cacgttcacg
     1081 acgagggcga cgtcgcccag ccgggcgatg gtggacgcgg cgtctcccgt tatggcgacg
     1141 agcgggatgt cgaggcgctt cacccagggt atgatgctga gcacctcctc ggtctccccg
     1201 ctgttgctga tcacgatgag cacgtctccg cgctggagca ttcccaggtc gccgtggagg
     1261 gagtcagcgg ggtggacgaa gagtgccggc gtgcccgtgc tcgacaacgt ggcggcgatc
     1321 ttcttgccga cgagtcccga cttgcccatg cccgtcagga cgaccctgcc cgtgcacccg
     1381 aatatggtct ccgccgccct gttaaaggag tcatcgaggc cttccatcac cttcaggagg
     1441 gcctccgcct ccttcctcag gatgtccttt cccgttctca cgtggtccac agggcctcct
     1501 ctatcctcct gagggtccgc aggacgcctt cgagctcgtc gagagggagg gaattgtcac
     1561 cgtcgcagag ggcccgtgcc ggatcgggat ggacctccat gaatacccct tccacgccga
     1621 cggctaccgc cgcccgcgcc aggggaacga tgaagcgggc ctccccgccg gaacggtccg
     1681 tcatggccga gggctgctgc acgctgtgcg tggcatcgaa gaccacggga tacccgaagt
     1741 cccgcatgat gggtattgcc cggaaatcat tgacgagggt atggtacccg aaggacgtgc
     1801 cccgttcggt gaccagtatg ctcctgttgc ccgtggattc cacctttctg atggcgtggg
     1861 ccatgtcacc gggggagagg aactggccct tcttgatgtt gacgaccctg cccgttctgc
     1921 cgcaggccgt gagtatgtcc gtctgccggg acaggagcgc cggcacctgg aggacgtcgg
     1981 cgacctcggc cgccgccgcg gcctcccccg cggaatggac atcagtgagg acggggacac
     2041 ccacctcgcc cttgacctgt tggaggatgg caagcccctc gtccagtccc ggccccctga
     2101 agcccgatat ggacgtcctg ttcgccttgt cgtaggagga cttgaatatg aagggtatgc
     2161 caagtccggc cgttatgcgc gcgatgctct cggccgtcct cagggatatg tccctgcctt
     2221 cgatgacaca ggggccgcct atgaagacga aaggcctgcg gccgacggtg acgttgccga
     2281 tggttatttc tttttgcact tcagtctttc ctgccttcgt ttcagcgagg ccttgataa
//
LOCUS       DAOPOA010000374         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_49667_length_2338_cov_4.250548, whole genome shotgun sequence.
ACCESSION   DAOPOA010000374 DAOPOA010000000
VERSION     DAOPOA010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49667_length_2338_cov_4.250548"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKJ17_08015"
     CDS             <1..250
                     /locus_tag="PKJ17_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75957.1"
                     /translation="INFTEAEKDIKKTEAIINSMTSKERARPNIIDGSRRLRISRGSG
                     TQVQDVNDLLRKYMETKKMLKKFTKGGAKGLQRQLFSR"
     gene            262..516
                     /gene="rpsP"
                     /locus_tag="PKJ17_08020"
     CDS             262..516
                     /gene="rpsP"
                     /locus_tag="PKJ17_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOD75958.1"
                     /translation="MAVKFRLSRFGAKKKPFYRIVVADERSPRDGRFIDKVGFYDPLK
                     NPAEISLDKEKIKTWYEKGVRPTRTVESLFKREGVLKELS"
     gene            535..768
                     /locus_tag="PKJ17_08025"
     CDS             535..768
                     /locus_tag="PKJ17_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOD75959.1"
                     /translation="MLKELIEYIAKALVDNPDLVRVSEIEGEKTSVIELNVAKDDLGK
                     VIGKQGRTARAMRTILSAASTKVKKRAVLEIIE"
     gene            768..1265
                     /gene="rimM"
                     /locus_tag="PKJ17_08030"
     CDS             768..1265
                     /gene="rimM"
                     /locus_tag="PKJ17_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOD75960.1"
                     /translation="MKWIPVGRVVSTFGIRGELKFHYYNEVKEELLDYTSLFALKGSE
                     FLELKPEGARYRKRLFYVSFQGFSTVEEVSFLVGKELFVREEDLPRLEEGEYYEYQLI
                     GLEVTTVQGSRLGKVRSLMHTGASDILVVEGESELMIPMVEGFIIDIDVSGGTVRVDV
                     EGLIP"
     gene            1262..1996
                     /gene="trmD"
                     /locus_tag="PKJ17_08035"
     CDS             1262..1996
                     /gene="trmD"
                     /locus_tag="PKJ17_08035"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOD75961.1"
                     /translation="MIFSVLTLFPGIFASPLKESILGKAAAKGLVGFNIINIRDFADD
                     PHRSCDDTPYGGGPGMVMKVEPIFRAIEHVKEVHGRARFVLLSPQGRSFDQFTAERFA
                     RSSHICLICGRYEGVDARVLEYVDEEISVGDYVTSGGEFPALVVIDAVCRFIPGVLGN
                     DESVSAESFREPLLEYPQYTRPETFMGSSVPPVLLSGNHEEIRKWRRKEAIKKTVLRR
                     SDLLQGFEPTEEDRRFMREIMEEFPE"
     gene            2049..>2338
                     /locus_tag="PKJ17_08040"
     CDS             2049..>2338
                     /locus_tag="PKJ17_08040"
                     /inference="COORDINATES: protein motif:HMM:NF021455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOD75962.1"
                     /translation="MATSLTNLEIHDMARTCMTFGLDMCYIVSPLARQREIAERLIHH
                     WIHGYGATYNPARGEALKKVRIRSDIGAMIGDFGSGPRPLVIGTSSRKREGKT"
BASE COUNT          572 a          560 c          734 g          472 t
ORIGIN      
        1 catcaacttc accgaagccg aaaaggacat aaagaagacg gaagcgatca tcaattccat
       61 gacatcgaag gaaagggctc gccccaatat catagatggc agccgaaggc tgcgcatctc
      121 caggggcagc gggacccagg tgcaggacgt gaacgacctg ttgagaaaat acatggaaac
      181 gaagaagatg ctgaagaagt tcacgaaggg aggcgcgaag ggcctccaga gacaactttt
      241 ctcacgatag gaggtgggat cttggctgta aaattcagat tgtcgaggtt tggcgcaaag
      301 aagaaacctt tttaccgcat tgttgtggcc gatgagcgtt cccccaggga cgggaggttc
      361 atcgataagg tcgggttcta cgatccactg aagaaccccg cggagataag cctcgacaag
      421 gaaaagatca aaacgtggta cgaaaaaggt gtacggccga cgaggaccgt tgaaagtctg
      481 ttcaagagag agggggttct gaaggagctt tcataaaacc aacggaggtg gatcgtgttg
      541 aaagagttga tcgagtacat tgcgaaggcg ttagttgaca atcctgatct ggtcagggtc
      601 tccgagatcg aaggcgagaa gacgtcggtc attgagctca atgttgcgaa ggatgacctc
      661 gggaaggtca tcgggaaaca gggcagaaca gccagggcaa tgaggacgat cctgagcgcg
      721 gcttcgacga aggtgaagaa gagagcggtc ctcgagatca tcgaataatg aagtggatcc
      781 ctgtcggccg tgtcgtgtcg acattcggca tacggggtga gctcaagttt cactattaca
      841 acgaggtgaa ggaagaatta ctcgactaca cctcgttgtt tgcccttaaa ggcagtgagt
      901 tcctggaact taagccggaa ggggcaagat acaggaaacg cttgttctat gtttccttcc
      961 aggggttctc aacggtcgaa gaggtctcat tcctggtggg gaaggagctt tttgtccggg
     1021 aggaagacct tccgcggttg gaggaaggcg agtattacga gtaccaattg atcggtcttg
     1081 aggtgacgac cgtgcagggg tcgcgtcttg gaaaggtccg gtcgctcatg cacacgggtg
     1141 ccagtgacat ccttgtcgtc gaaggagaaa gtgaactgat gatccccatg gtggaaggat
     1201 tcatcatcga tatagacgtg agcggcggta cagtccgcgt ggacgttgaa gggctcatcc
     1261 cgtgatcttc tcggtcctca cattgttccc gggcatattc gcgtcgcccc tcaaggagag
     1321 catactcggc aaggcggccg ccaagggcct tgtcgggttc aatatcatca acatacgcga
     1381 ttttgccgat gaccctcacc gctcctgcga cgacacgccc tacggcggtg ggccggggat
     1441 ggtcatgaag gttgaaccca tcttcagggc catagagcac gtaaaggagg tccacgggag
     1501 ggccaggttc gtcctccttt cgccgcaggg cagaagtttc gaccagttca cggccgagcg
     1561 cttcgcccgg tcgtcccaca tttgtctcat ctgcggccga tacgagggcg tcgacgcgcg
     1621 cgtgctcgag tatgtcgatg aggagatatc ggtgggggac tacgtcacat cgggcgggga
     1681 gttcccggcg cttgtcgtga tcgatgccgt ctgccgtttc atccccgggg tcctgggaaa
     1741 tgatgaatcg gtatcagcgg agagtttccg ggagcctctc ctggagtacc cgcagtacac
     1801 gaggcctgaa acgttcatgg gttcctcggt gccgcccgtc ctcctgtcgg ggaaccatga
     1861 ggagatacgg aagtggcgtc gcaaggaggc gataaagaaa acggtcctga ggcgttccga
     1921 cctgctccag gggttcgagc ctaccgagga ggaccgcagg ttcatgagag agatcatgga
     1981 ggagttccct gaataacgta agcatcgggg tcattcatta tcccgtttac gacaagcggg
     2041 gagacgttgt ggcaacgagc ctcacgaacc tcgagataca tgatatggca agaacttgca
     2101 tgacttttgg tctggacatg tgctatattg tgtcacctct cgccagacag cgggagatag
     2161 cggaaaggct gatccatcac tggatacacg gatacggggc aacgtacaac cccgcacggg
     2221 gagaggccct caagaaggtg aggatcagat cggacatcgg cgcgatgatc ggggatttcg
     2281 gcagcgggcc aagacccctc gttatcggta catcctctcg caaaagggag ggtaagac
//
LOCUS       DAOPOA010000375         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_49757_length_2335_cov_3.416228, whole genome shotgun sequence.
ACCESSION   DAOPOA010000375 DAOPOA010000000
VERSION     DAOPOA010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49757_length_2335_cov_3.416228"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PKJ17_08045"
     CDS             complement(<1..1163)
                     /locus_tag="PKJ17_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOD75963.1"
                     /translation="MKSFSLPIKWKLTLWYGGILALILVTFSSGVYIYFQNSLQKSID
                     TKIKSIAEVLASSMTETHGQSLFGNFERYLENALGKKPKGKFIQIIDASGKIGARLND
                     LEAEAVPVSFVTLERALKGETVYETLESARPRLRMITMPILDSKKVTSIVQVGSSLEE
                     FEDTMKRLLIIMIISIITSISATIVVGYFMAKKTMKPVDQIRRAAVKISSTNLDERID
                     IKKGRKDELGRLAETFNAMISRLKDSFQRINQFSIDVSHELKTPLTILKGETELALRK
                     ERTNQEYRRSMQSNLEEIDRMSRIIDDLLLLSKAETKDIGMNLEKVDLRDLLAGVCLN
                     MKLFGENKGVEVVVRDLEDIRIVGDELKLRRMITNVVENGIKYGHPGGHVTVSS"
     gene            complement(1150..1812)
                     /locus_tag="PKJ17_08050"
     CDS             complement(1150..1812)
                     /locus_tag="PKJ17_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOD75964.1"
                     /translation="MEDESKVANFVSRGLEEEGYGVDVAGDGRTGLDLVTTRDYDIVL
                     LDLMIPEIDGLEVLKRMRESGIETPVLIITAKSTKEDVIKGLDTGSDDYLTKPFSFEE
                     LLARIRALLRRSRKAVPLTLEYREIVLNPYNRRLSIGGKEAELTEKEFLIMEYMVRNS
                     ERPLARKEMAEYAWQSQDDSSNIVDVYVNFLRKKIELLTARKFIHTVRGVGYILKEDE
                     KL"
BASE COUNT          456 a          638 c          641 g          600 t
ORIGIN      
        1 gatgagaccg tcacgtgccc gccggggtgt ccgtacttga tgccgttctc caccacgttg
       61 gttatcatcc tgcgcagttt gagctcgtcc ccgacgatgc ggatgtcctc gaggtccctc
      121 acgaccactt ccaccccttt gttctcaccg aaaagcttca tattgaggca gacacccgca
      181 agaaggtccc tgagatcgac cttctcgagg ttcatgccga tgtccttcgt ttcggccttg
      241 gaaagcagga gcaggtcgtc gatgatccgg ctcatcctgt cgatctcctc gagattgctc
      301 tgcatggagc gcctgtattc ctggttcgtc ctttccttcc tcagggccag ctccgtttcg
      361 cctttcagaa tggtaagcgg tgtcttgagc tcgtgggaca cgtcgatgct gaactggttg
      421 atcctctgga aggagtcctt gagcctcgag atcatggcgt tgaaggtctc cgcgagacgg
      481 ccgagttcgt cctttcgccc cttctttata tcgatccgct cgtcgaggtt ggtcgaggat
      541 atcttgacgg cggccctgcg gatctgatcc acgggcttca tggtcttttt cgccatgaag
      601 tatccgacga ctatggtggc gctgatggag gtgatgatgc tgatgatcat gattatgagg
      661 agacgcttca tcgtgtcctc gaactcttcc agggacgaac ccacctggac gatgctggtg
      721 accttcttgg agtccaatat cggcatcgtg atcatgcgca gccggggacg ggccgactcc
      781 agcgtttcat agacggtctc tcccttgagc gccctctcga gagtgacgaa gctgacggga
      841 acggcctcgg cctcgaggtc gttcagcctc gccccgatct tgccggaggc gtctatgatc
      901 tggatgaact tgcccttcgg cttcttgccg agggcgttct cgagatagcg ctcgaaattt
      961 ccgaagaggc tctggccgtg ggtctccgtc atcgacgagg cgaggacctc cgcgatcgac
     1021 ttgatcttgg tgtcgatgct tttctgcagg ctgttctgga aatatatgta gaccccgctg
     1081 gagaaggtga ccagtatcag ggccaggatg ccgccgtacc agagggtcag tttccacttg
     1141 atggggagac taaagctttt catcttcctt gaggatgtag cccaccccgc gaacggtgtg
     1201 gatgaacttc cttgccgtga ggagttctat cttcttgcgg agaaaattca cgtagacatc
     1261 gacgatgttt gaggaatcgt cctggctctg ccaggcatat tcggccattt ccttcctggc
     1321 gagcggcctc tcgctgttgc gaaccatgta ctccatgatg aggaattcct tttccgtgag
     1381 ctcggcctcc ttgcccccta tggaaagccg cctgttgtat gggttgagga cgatctcccg
     1441 gtactcgaga gtgagcggaa cggccttcct gcttctcctc aggagcgccc gtatcctggc
     1501 caggagttct tcgaaggaga aaggcttggt caggtaatcg tcgctgcccg tgtcgagccc
     1561 cttgatcacg tcctccttcg tgctcttcgc ggtgatgatg aggaccggcg tctctatgcc
     1621 cgactcgcgc atccgcttga gcacctcgag accgtcgatc tccggtatca tgagatcgag
     1681 gagaacgatg tcgtaatccc gcgtggtgac gagatcaaga cccgtcctgc cgtcgccggc
     1741 cacatccacg ccataccctt cttcttcgag gcccctcgac acgaaattgg cgaccttcga
     1801 ttcatcttcc acgagaagaa ctttcatatc cgtaataata tagtataaaa agaaccattg
     1861 tacaattctt ccgtttcagc ggcacccccg ccgccccatc gacagacaac aaaaaccgtt
     1921 tcaggtttca ggtttcaggt ttcaggtttc aggtttcagg aagagagacg gcgaaggggg
     1981 atgggatgtc aacgtgattg gtggatggag gtcggatttt cttgcttgcc gacgtgcagc
     2041 gatcctgccg gaatgtagac ttcccgtcct tgtctttgac ttgaaacgtg gaacgtgaaa
     2101 cctgaaacgg tcttttctgt gaatttcttt gacaaaacgg ggggtgatgc tattaagtat
     2161 taaggtttcc gggtgaacaa gagggatatc ttccaaaaca aaggcagaat acatgaaatg
     2221 acacagggag gtctgcatcc atgagtcttc ttgaagcgta cattattact ttcatcgcga
     2281 gtttctgtac ccttgtgatc gaaatggtcg cgggacgcat cctcgcgcct ttcgt
//
LOCUS       DAOPOA010000376         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_49854_length_2331_cov_4.225395, whole genome shotgun sequence.
ACCESSION   DAOPOA010000376 DAOPOA010000000
VERSION     DAOPOA010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49854_length_2331_cov_4.225395"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(475..975)
                     /locus_tag="PKJ17_08055"
     CDS             complement(475..975)
                     /locus_tag="PKJ17_08055"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOD75965.1"
                     /translation="MHLVIDGYNYINRINASSVQKGASMDLLRRDFLERCARYKKIRS
                     ARITVVFDAWDTPAPGRHSENVRGIEVIYSRQGETADDVIIGWIRKRPAGLIVVSSDR
                     AIIDEAKARGVAFLTPVKLAEMMAAPAFETKEEDDYDEAPHPKKGNPRKLPKKVRKAV
                     KAIDKL"
     gene            complement(1155..1571)
                     /gene="nusB"
                     /locus_tag="PKJ17_08060"
     CDS             complement(1155..1571)
                     /gene="nusB"
                     /locus_tag="PKJ17_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOD75966.1"
                     /translation="MRRRKARELALRMLYQVETAGEDPERALTKYCSLFPYQQDIVDY
                     ARLLLTGISAHQEQIDEFIRKACEHWRIDRIAYVDKNVLRIGAFEMIFSGDVPPKVAI
                     DEAIEMGKKYGSEDSREFINGVLDRILKDFYGEKKF"
     gene            complement(1544..2032)
                     /gene="ribE"
                     /locus_tag="PKJ17_08065"
     CDS             complement(1544..2032)
                     /gene="ribE"
                     /locus_tag="PKJ17_08065"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF000812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOD75967.1"
                     /translation="MVREGKLNAKGFRFAIVVSRFNSFITDRLVEGALDALKRHGADE
                     NKIDIYRVPGSFEIPLAAKLLAKKKDVDAVVCLGAVIKGATPHFHYVASEVTKGIAQS
                     SLELEKPIAFGIITSDSIEQAIERAGTKAGNKGFDAAMSAIEMVNLIKESNLCADGKR
                     ES"
     gene            complement(2042..>2331)
                     /locus_tag="PKJ17_08070"
     CDS             complement(2042..>2331)
                     /locus_tag="PKJ17_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75968.1"
                     /translation="KAYSLQDCGHDTVEANIALGFSPDLRDYGIGAQILRDIGVRKMR
                     LLTNNPKKIKGLEGYGLTVVERVSIEMVPTKDNIAYLTTKQKKLGHILNNI"
BASE COUNT          458 a          632 c          631 g          610 t
ORIGIN      
        1 gaagccgccg aatgtaacat cgtacctgct tgtcatgctg cctgcgttgc tcactgccgg
       61 gatgaggagg agcagggcaa gcagaaggaa agctgttctt ttcatggttt ccccctttgg
      121 gatatttttt gaaatctctg cccaaactac tttctttgga aatggtcttt gagattactc
      181 tttgaaggcg cccttgcggg cggtctttga aattactggt gcggcttttt gatgcctacg
      241 atagtgtgcc ggtccacctc cttcattaat tttttttaat ttttatcagc gacctcgacg
      301 cttgtcaagg gcaatgaaag ggggtaggga cgaagggtca gaaggtgggg acgagagggg
      361 caaaaaaatg ttctagagac ggtttcaggt ttcaagttcc aggtttcagg ttaaagacag
      421 aaacaggaac ctctatgccg gtgtttctct cagcctgaaa cctgaaacgg tctcttacag
      481 cttgtctatc gcttttacgg ctttgcggac ctttttgggg agtttacggg ggttgccttt
      541 tttggggtgg ggggcttcgt cgtaatcgtc ctcctctttt gtctcgaagg cgggcgcggc
      601 catcatttcg gcaagcttca cgggggtgag gaacgcgacg ccccgcgcct tcgcctcgtc
      661 gatgatggcg cggtcggagc tgaccacgat gaggcccgcc ggccgtttgc ggatccagcc
      721 gatgatgacg tcatccgccg tctcgccctg ccgggaatag atgacctcta tccccctcac
      781 gttctcgctg tgccttccgg gcgcaggggt gtcccaggca tcgaagacga cggttatccg
      841 cgcggacctg atcttcttgt accgcgcgca cctttccagg aagtccctcc ggagaaggtc
      901 catggacgcc cctttctgca cggaggaggc gttgatccga ttgatgtaat tataaccgtc
      961 gatgacgaga tgcataaaaa aacccgtttt aagttttagg ttttaagttt taagtaaaag
     1021 accaaagaca gaaacaaaga cggttttaag tgttaagttg aagaccggaa gaaagggagc
     1081 cttttcgggg aataccgaag catccgctat gccgccggtc tcttgcttaa aacttaaaac
     1141 gtagaactta aaacctaaaa cttcttctcc ccatagaaat ccttgaggat cctgtccagg
     1201 acgccgttta tgaattcgcg ggagtcttcg ctgccgtatt tcttgcccat ctcgatggcc
     1261 tcgtctatgg cgaccttcgg ggggacgtcg ccggagaata tcatctcgaa ggccccgatc
     1321 ctgagaacat tcttgtccac ataggcgatg cggtctatcc tccagtgctc gcacgccttc
     1381 cggatgaatt cgtctatctg ctcctgatgg gcgctgatcc cggtaagaag aagtctcgcg
     1441 taatcgacga tatcctgctg gtacggaaag agcgagcaat acttggtgag ggcgcgctcc
     1501 gggtcttccc cggccgtctc cacctggtag agcatcctca gggctagctc tctcgctttc
     1561 cgtctgcgca caagttgctt tccttgatga ggttcaccat ctcgatggcc gacatggcgg
     1621 cgtcaaatcc cttgttgccc gccttcgtgc cggctcgctc gatggcctgt tctatgctgt
     1681 cgctcgtgat gatcccgaag gcgatgggtt tctccaactc gagggatgat tgagcgatcc
     1741 ccttcgtcac ttccgacgct acgtagtgaa agtgaggcgt cgctcccttg atgacggcgc
     1801 cgagacagac gacggcgtcg acgtctttct tctttgccag cagtttcgcc gccagcggga
     1861 tctcgaagga accgggtacc cggtagatgt cgatcttgtt ctcgtcggcc ccgtgcctct
     1921 tcaacgcatc gagggcgcct tcgaccaggc ggtccgttat gaagctgttg aacctgctta
     1981 cgacgattgc aaacctgaag ccttttgcgt tgagcttgcc ctcccgtacc atgtccaccc
     2041 cttatatgtt gtttaggata tgacccagct ttttctgctt ggtcgtaaga taggctatgt
     2101 tgtccttcgt cggcaccatt tctatggaaa ccctctccac gacggtcaaa ccatatccct
     2161 caagaccctt tatcttcttc gggttgttcg tgagaaggcg catcttccgg acgccgatgt
     2221 ccctgagtat ctgggcgccg atcccgtagt ccctcaggtc gggtgagaaa ccaagcgcga
     2281 tgttcgcctc gacggtgtca tgcccgcagt cctgcaggct gtacgccttg a
//
LOCUS       DAOPOA010000377         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_49859_length_2331_cov_3.509227, whole genome shotgun sequence.
ACCESSION   DAOPOA010000377 DAOPOA010000000
VERSION     DAOPOA010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49859_length_2331_cov_3.509227"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2022
                     /gene="fusA"
                     /locus_tag="PKJ17_08075"
     CDS             <1..2022
                     /gene="fusA"
                     /locus_tag="PKJ17_08075"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOD75969.1"
                     /translation="VEAMLFLAGENTRLGSVDEGTSLMDYEPEEVERKITISSTLACF
                     EWAKNKINLLDTPGDDNFISDAKLCMRVVDTAVIVMSAVSGVKVGTEKVWGYADEFGA
                     AIAIFVNKMDRERADFGRTVEDIQKNLTDRTCLVVQIPVGQEESFKGVVDLLAMKACI
                     FKGDGSGGFDVTDIPAEQLDLAQKYREKLVETAVEMDDEVMERYLNGDEIGPEEIEKC
                     LKAGIAGNKIVPIFCGSAAGNIGISTLLDGIVKLFPAPSFKKEVEAINPKTGEKTTRE
                     SSVSQPFSALIFKTITDPFAGKLTLFRVYSGELHPDMTVLNVLKDEKERIGQIFSLVG
                     KKQKPAGSASAGDIAVVAKLRDVQTGDTICDEKSPIRYEGVTVPNPVISFSLQPKAKG
                     DEDKLNTSLARLIEEDQTIKYSRDEQTKEFLLSGMGQVHIEVIVERMKRKFGVEVELK
                     DPKVPYKETIKGTTKIQGKYKKQSGGKGQYGDTWLELGPKPRGEGFEFVDKIVGGAIP
                     RQYIPAVEKGIVEAMNQGILAGYPVVDFRVTLYDGSYHDVDSSEMAFKIAASMGFKKG
                     LEMCKPTLLEPIMKMEVIVPDENMGDIMGDMNSRRGKIMGVETKGSNQVVKAVVPMAE
                     VLRYAPDLRSMTGGRGTFTMDFSHYEEVPAQIADKIVEAAKREKEAQEK"
     gene            2137..>2331
                     /locus_tag="PKJ17_08080"
     CDS             2137..>2331
                     /locus_tag="PKJ17_08080"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOD75970.1"
                     /translation="MRAVIQRVKSASVEVDGRCVGQTGPGLLVFLGIAKGDGEKDIDW
                     LIEKIVNLRIFEKEDGKFDES"
BASE COUNT          567 a          589 c          756 g          419 t
ORIGIN      
        1 gtggaagcca tgctctttct tgccggggag aacacgaggc tcggcagcgt agacgaaggc
       61 acctctctta tggactacga gccggaggaa gtggagagga agatcacgat atcctccacc
      121 ctggcttgct tcgaatgggc aaagaacaag attaacctcc tcgatacccc gggtgacgac
      181 aattttatct ccgacgcgaa gctgtgcatg agggttgtcg atacggcggt gatcgtcatg
      241 agcgctgtat cgggtgtgaa ggttggaacg gaaaaggtct ggggatatgc cgacgagttc
      301 ggggcggcga tcgccatctt cgtgaacaag atggaccggg aacgcgccga ctttggcagg
      361 accgtggagg acatccagaa gaacctgacg gacaggacct gtctcgtcgt acagatcccg
      421 gttggtcagg aagagagctt caagggagtc gtggacctgc tcgccatgaa ggcctgcatt
      481 ttcaaggggg acggctcggg cggtttcgat gttacggaca taccggccga gcagctcgat
      541 ctcgctcaga agtatcgcga gaaactcgtg gagacagccg tggagatgga tgacgaggtc
      601 atggagcggt acctgaacgg cgatgagata ggccccgagg agatcgagaa atgcctcaag
      661 gcgggcattg ccggcaacaa gatcgttccc attttctgcg gttcagcggc ggggaacata
      721 ggcatcagta ccctgcttga cggcatagtg aaacttttcc ctgctccttc gttcaagaaa
      781 gaggtcgagg cgattaaccc gaagacgggc gagaagacga cgcgggagtc atcggtcagc
      841 cagcccttca gcgccctcat cttcaagacc atcaccgatc ccttcgcggg gaagttgacg
      901 ctcttcaggg tctattcagg ggagttgcat cccgatatga cggtattgaa cgtcctcaag
      961 gacgagaagg aacggatcgg acagatcttc tctctcgtgg gaaagaaaca gaaacccgca
     1021 ggttccgcga gcgcgggcga catagccgtg gtggcgaagc tcagggacgt gcagacgggc
     1081 gataccatct gcgacgagaa atcacccatc aggtacgagg gagtgacggt gccgaacccg
     1141 gtcatatcct tctccctgca gcccaaggcc aagggcgatg aagacaagct gaacacgtcg
     1201 ctggcgcggc tcatcgaaga agatcagacc atcaaatact cccgcgacga gcagacaaag
     1261 gagtttcttc tctccggcat gggccaggtc catatcgagg tcatcgtgga gaggatgaag
     1321 cgcaagttcg gcgtcgaggt cgagctgaag gacccgaagg tcccctacaa ggaaacgatc
     1381 aagggaacca ccaagatcca gggaaaatac aagaaacagt ccggcggcaa gggccagtac
     1441 ggcgacacgt ggctggagct cggtcccaag ccccgcggcg agggtttcga attcgtggac
     1501 aagatcgtgg gcggggccat tcccaggcag tacattcccg ccgtcgaaaa gggcatcgtg
     1561 gaggccatga accagggtat cctggcgggc tatcccgtcg tcgatttcag ggtcaccctt
     1621 tatgacggct cctatcacga cgtcgactcg tcggaaatgg cattcaagat agcggcgtcg
     1681 atgggtttca agaagggtct cgagatgtgc aagccgactc tcctcgagcc catcatgaag
     1741 atggaagtga tcgtgcctga cgagaacatg ggggatatca tgggcgacat gaactcccgt
     1801 aggggcaaga tcatgggcgt ggagaccaag gggagcaacc aggtggtcaa ggcggtggtg
     1861 cccatggccg aggtgctgag gtacgcgccc gacctgaggt caatgaccgg tgggcgggga
     1921 acattcacca tggatttctc ccactacgag gaagttcccg cacagatcgc ggacaagata
     1981 gtcgaggctg caaagcgcga gaaagaggcg caggagaaat agagccaccg tgcgcggata
     2041 cataatggta tgagatgggc ccctggaaaa ggggcccttt tgctgttccc attccggaaa
     2101 gcctgagggg gcgcaggaac ctgataagga ggacagatgc gggccgttat ccagcgagtc
     2161 aagagtgctt ccgttgaggt tgatggacgg tgtgtcgggc agacaggacc cggcctcctc
     2221 gtctttctcg gtatcgccaa gggtgacggc gagaaggata tcgactggct gatcgagaag
     2281 atcgtgaacc tgaggatctt cgagaaggag gacggcaagt tcgatgaatc c
//
LOCUS       DAOPOA010000378         2330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_49894_length_2330_cov_2.928791, whole genome shotgun sequence.
ACCESSION   DAOPOA010000378 DAOPOA010000000
VERSION     DAOPOA010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49894_length_2330_cov_2.928791"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKJ17_08085"
     CDS             <1..810
                     /locus_tag="PKJ17_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOD75971.1"
                     /translation="TPFVAGGEIVGAVGSFVDITKMKMSEESIKTLNAELAKNLVLVE
                     DTNKELEGFVHSVTHDLRSPLVVINGFSRRLLDFTEEEFGQKSHDYIEYIRETSQNAL
                     FLVRDLLRLFKIARGEMNRTTVDLAEMSRSLRQYFGGLYPRSSLRLTAPDTLLVTGDY
                     SLLKIVMENLLDNSYKFTARNEGETIIEIGSRRENGDRVIFVRDNGCGFDVSRASCIF
                     EPFSRFHKETEYRGNGIGLATVKRIVHRHGGRIWAESQPGRGATFCFTLPD"
     gene            complement(863..1186)
                     /locus_tag="PKJ17_08090"
     CDS             complement(863..1186)
                     /locus_tag="PKJ17_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75972.1"
                     /translation="MKRSVFSTTMVIFVLLLFASTAVSMNHGMTKSMTGKVIGVDENG
                     KGIAISSKAGGQEIVAGAIVTDATVVHVKGKKANIMDIKAGDTVTLTYSYENNDLYAK
                     KVVKR"
     gene            complement(1528..1713)
                     /locus_tag="PKJ17_08095"
     CDS             complement(1528..1713)
                     /locus_tag="PKJ17_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75973.1"
                     /translation="MVAMPKQGEKVRSKFNGREFIVELVGERMIVLQERREAIRFVTT
                     IDNLRSFYELDKDRSAP"
     gene            2031..>2330
                     /locus_tag="PKJ17_08100"
     CDS             2031..>2330
                     /locus_tag="PKJ17_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD75974.1"
                     /translation="MIDDEVRWCELLRETLASEGFDVAVAGDSALASQLIHQVDYDVI
                     LTDLRMDGKNGLQLLEETKKVAPTTPVILITAFGSIDSAVQAMKMGAYDFITKSGD"
BASE COUNT          502 a          641 c          646 g          541 t
ORIGIN      
        1 acgccctttg tggcgggcgg agaaatagtc ggggccgtcg ggagttttgt cgatatcacc
       61 aaaatgaaga tgtccgagga gtcgatcaag accctcaacg cggaattggc caagaacctg
      121 gtcctggtgg aggacaccaa caaggagttg gaagggttcg tgcattccgt cacccacgac
      181 ctgaggtcgc cgctcgtggt catcaacggg ttcagtcgac ggctcctcga ttttaccgag
      241 gaagagttcg gccagaagag ccacgattac atcgaatata tcagggaaac gagccagaac
      301 gcgttgtttt tggtcaggga ccttctgagg cttttcaaga tagcccgagg ggaaatgaac
      361 aggaccacgg tggatctggc ggagatgtcc cggtcgctgc ggcagtattt cggaggtctc
      421 tatccccgca gctcgctgag gctgaccgcc ccggatacct tgttggtgac gggggactac
      481 agccttctca agatcgtcat ggagaacctg ctggacaact cctacaagtt caccgccagg
      541 aatgagggtg agacgatcat tgagataggg tcgcgccgtg agaacgggga ccgggtcatc
      601 ttcgtcaggg acaacggatg cgggttcgat gtttcccggg ccagctgcat cttcgaacct
      661 ttttcccggt tccacaagga gacggagtac aggggtaacg ggatcggtct cgcgacggtg
      721 aaaaggatcg tccaccgcca cggcgggcgc atttgggccg aaagccagcc tggcaggggc
      781 gcaacgttct gcttcaccct gcctgactag aaaacgtgcg aaaccccggt cgtcccgacc
      841 cggggtgcag gtcagccctt catcacctct tcacgacctt tttcgcgtaa aggtcgttgt
      901 tttcgtatga atacgtgaga gttaccgtgt cgcctgcctt gatgtccata atgttcgcct
      961 tcttgccctt gacatggacc accgtcgcgt ctgttacgat ggcgccggcg acgatctcct
     1021 gacctccggc ctttgacgag atcgcgatac cctttccgtt ctcgtcgaca ccgataacct
     1081 tgcctgtcat cgactttgtc attccgtggt tcatggacac cgctgtcgat gcgaagagca
     1141 aaagaacaaa gatcaccatg gtggtgctga agacggatcg tttcatagat ttcctcctct
     1201 ttctgtgatt tgggggcatg ctgtaagctc cctgtatact aaaatgaagc aaaacatcta
     1261 ccaggcagcg cttgcgccgt aacgagtcgg aaggaccggg gtgaacaggg gccttttgcc
     1321 cctgacagtg atcgtaatgc cccgacccgc cctgtcgcct tcatgaatca tccccgcatc
     1381 cgaccgtgac atccagtcac gggctctcag ggatttcagg acatcaccgc cgacgcgaga
     1441 agaaggagca ggaaaatcgt ccacataacg gaataaactc gccctttcat acggtccctc
     1501 ctttttgcct gtgttgtttc cacgacatca gggagcgctc cggtctttgt ctagttcgta
     1561 gaatgaccgc agattatcga tcgtcgtgac gaagcgtatg gcttcccgtc tttcctggag
     1621 cactatcatt ctctcaccca ccagttcgac aatgaattcc cttccgttaa acttgcttct
     1681 gaccttttct ccctgtttag gcatagctac catgcatctc cctccgttgt cgtgcgaaaa
     1741 acttcttgtc ctcctgctca agcaaaacgt gtaccacata ccctttttgt tgaaaggaca
     1801 agagaaaaca cggtgaaagc atctttgcgg aggcaggcag acagggggtt ttttgcccct
     1861 catagtggtg ttatgcccca gcccgccctt tacgattcga tcataatagg gtagaatgga
     1921 atctgaaggt tcttcccggt ccgcgtacga gggtcgtccc ccggtgtgaa aagggcgttg
     1981 ccggtcttca aggaggtgtt cccctgaagg acaggtttca caggatactg gtgatcgacg
     2041 acgaggtgag atggtgcgag ctgttgcggg agacgcttgc ctctgagggt tttgacgttg
     2101 ccgttgccgg tgacagcgcc ctggcctccc agctcataca tcaggtcgat tacgacgtca
     2161 tcctcaccga tctcaggatg gacgggaaga acgggctgca gcttcttgaa gagacgaaga
     2221 aggttgcccc tacgacaccc gtcattctga tcaccgcctt cgggtccatc gattcggccg
     2281 tccaggccat gaagatgggc gcctacgatt tcataacgaa gagcggtgat
//
LOCUS       DAOPOA010000379         2327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_49964_length_2327_cov_3.393046, whole genome shotgun sequence.
ACCESSION   DAOPOA010000379 DAOPOA010000000
VERSION     DAOPOA010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49964_length_2327_cov_3.393046"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="PKJ17_08105"
     CDS             <1..1606
                     /locus_tag="PKJ17_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD75975.1"
                     /translation="GLGYLDDRGRVVVASSTQNIHYKMKEISRLLGIPTEEIRVVQAT
                     TGGGFGGKLDVTVEGYLALAVWHTKRPVFMRYTREESLLAQTKRHPLYIVYKTGARKD
                     GSLTAVEVNIVGDTGPYNSYGETVCLRAAVHATGPYEVPNVLVNSRMFYTNNPVSGAM
                     RGFGVPQLALAHESQLDEIAGLIGMDPLDIRMKNALKRGSLTATSQALNHSAGLLETL
                     KAVEPFWRERRKEGPGSGFGLGCMYYGIGNTGISNPSSCYLTLTPEGRVEMHSGVCDI
                     GQGSDTVLLQILCDALGMEEMGIDLVRGDTDTSYDAGSTSASRQTYISGMAVCNAAQK
                     LKKYLKEHGHYDGKPLKEIYRDAGDKGVPIFEGHFDPPTTAPDPETSQGAPYATYAFA
                     THMTQVDVDEVTGVCKVIKVHAAHDVGRAINVSSVRGQIYGCVAMGIGLALMEEFIPG
                     KTGSFDNYYLPTSMDMPEVEAMIVEDREPTGPFGAKGVGEPALIPQAAAIVNAIKDAT
                     GVRMRQLPCDIERLKLALEKDKENKG"
     gene            1629..>2327
                     /locus_tag="PKJ17_08110"
     CDS             1629..>2327
                     /locus_tag="PKJ17_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD75976.1"
                     /translation="MSTLVIRNVGTIFTGDIERPVVNGPVSILIEDGKIKAIEGTEPK
                     ADKAIDANGMTVCPGFIDSHVHFVFGDFTPRQSQLGYIESSLHGGVTSMISAGEVHLP
                     GRPTDPKGTKALALLAQKSFAKLRPAGVKTYGGGLILEKGLVEADFKELAEDGVWLVG
                     EIGLGSIKAAEEARQMVEWAKKYGMKVLMHVGGTSVPGSSTVTCDDVLVAKPTVACHL
                     NGGPTSIPVPEVKRV"
BASE COUNT          503 a          702 c          749 g          373 t
ORIGIN      
        1 gggcctgggc tacctcgacg acagggggag ggtggtcgtc gcctcctcca cgcagaacat
       61 ccactacaag atgaaggaga tttcgaggtt gctcggcatc cccacggaag agattcgggt
      121 cgtccaggcc acgaccggtg ggggtttcgg cggcaagctc gacgtcaccg tcgaaggtta
      181 cctcgcactt gccgtctggc acacgaagag gcccgtcttc atgaggtaca cgagggagga
      241 aagcctcctc gcccagacaa agaggcatcc cctctacatc gtctacaaga cgggggccag
      301 aaaggacggc agcctcaccg ccgtcgaggt gaacatcgtc ggcgatacgg ggccctacaa
      361 ttcctacggc gagacggtct gtttacgggc ggccgtacac gcgacggggc cctacgaggt
      421 ccccaatgtc ctcgtgaact cacggatgtt ctacacgaat aaccccgtat cgggcgccat
      481 gcggggcttc ggcgtgcccc agctcgccct cgcccacgaa tcccagctcg acgagatcgc
      541 cgggctcatc ggcatggatc ccctcgacat acggatgaag aacgccctga agaggggttc
      601 gctgaccgcc acctcgcagg ccctgaacca cagcgcgggg ctccttgaga ccttgaaggc
      661 ggtggagcct ttctggaggg agcggcgcaa agagggaccg ggcagcggct tcggcctggg
      721 ttgcatgtac tatggcatcg gtaacacggg aatttctaac ccctcgagct gctatctcac
      781 cctcacgccc gagggcaggg tggagatgca ctccggggtc tgcgacatcg gccaggggtc
      841 ggacacggtg ctcctgcaga tactctgcga tgccctcggc atggaagaga tgggcatcga
      901 cctcgtgcgg ggcgatacgg acacgtccta tgacgccggt tccacctcgg caagccgcca
      961 gacctacata tcgggaatgg ccgtctgcaa cgcggcccag aagttgaaga agtacctgaa
     1021 ggaacacggg cactatgacg gtaagcccct caaagagatc tatcgcgacg ccggggacaa
     1081 gggcgtcccc atcttcgagg gccacttcga tccgcccacg acggcaccgg accccgagac
     1141 atcgcagggc gccccctacg ccacgtacgc cttcgcgaca catatgacgc aggtcgacgt
     1201 cgacgaggtg acgggcgtct gcaaggtcat caaggtgcac gcggcccacg acgtgggaag
     1261 ggccatcaat gtgagcagcg tgaggggaca gatctatggg tgtgtggcca tggggatcgg
     1321 cctcgcgctc atggaggagt tcatacccgg gaagactggg tccttcgaca attactatct
     1381 cccgacgtcc atggacatgc ccgaggtcga agcgatgatc gtcgaagacc gcgagcccac
     1441 gggacctttc ggggccaaag gggtcggcga accggccctc attccccagg ccgcggccat
     1501 cgtgaacgcg atcaaggacg cgacgggagt ccggatgcgc cagctaccat gcgacataga
     1561 gaggctgaag ctggccctgg agaaggataa agagaacaag ggatgaaccg agtgaaagga
     1621 ggaggcgtat gagcacgctc gttataagga atgtgggaac catcttcaca ggcgacatcg
     1681 aaagacctgt tgtgaacggt cctgtctcca tcctcatcga ggacgggaag atcaaggcca
     1741 tcgagggcac agagcctaag gcggacaagg ccatcgatgc caatggcatg acggtctgcc
     1801 cgggattcat cgattcccac gtgcacttcg tcttcggcga tttcactcct cgtcagagcc
     1861 agctcgggta tatcgaaagc agtctccatg gcggcgtgac atcgatgata tccgccggtg
     1921 aggtccacct gcccggcagg ccgacggacc ccaaggggac gaaggccctc gcgcttctgg
     1981 cccagaaatc cttcgccaag ctgaggccgg cgggcgttaa gacatacggc ggcggcctga
     2041 tactcgaaaa aggtctcgtc gaggccgact tcaaggaact cgccgaagat ggcgtgtggc
     2101 ttgtcggcga gatcggtctc gggagcataa aggcggccga agaggcgcgg cagatggtcg
     2161 agtgggcaaa gaagtacggc atgaaggtcc tcatgcacgt cggcggcacg tcggtgccgg
     2221 gaagctccac cgtgacctgc gacgacgtcc tcgtggcgaa acccaccgtg gcctgccatc
     2281 tgaacggcgg acccacgagc atccccgtac ccgaggtgaa gagggtc
//
LOCUS       DAOPOA010000380         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_50429_length_2311_cov_3.351950, whole genome shotgun sequence.
ACCESSION   DAOPOA010000380 DAOPOA010000000
VERSION     DAOPOA010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50429_length_2311_cov_3.351950"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /locus_tag="PKJ17_08115"
     CDS             <1..1691
                     /locus_tag="PKJ17_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD75977.1"
                     /translation="LTTMQLTGWTVVFAQNRDEVMAPVNRILWIMLVSGILFLIMTIT
                     TIVIVSSRLSSPVQRLLERMKKMTEHSTEIILQIGTDRKIAFANPAFERITGTRPEEV
                     IGTEPDFANPHNIPPDVIWDSLNSGTPWSGRVSLRAIDGSPVTLDVMVMPLRNSNGTV
                     EGYLEIGRDVTKELMFENKMQQSQKLEAIGTLAGGIAHDFNNILSGILGYAELALITG
                     KNDGETEKFLKEIIKAAQRAGELVGQILTFSRQTEVELRPILPGPILEEASKLLRASA
                     PASIDIRLRIDSRSAILAEPTQIHQIVMNLFTNAVHAIEPEHGTITIELEDLVVDDDL
                     AKAHPDMRPGRHIALSVSDTGSGMEQEIVDRIFEPFFTTRSQGKGTGLGLSVVHGIVK
                     ELGGMIELKSQAGKGTTFSIYIPCTDMDAAGPPREAPPTEKGRYRIVLIDDEEAILTT
                     TGAILTQLGHKVTTFANGLDALEAVKEDPHDFDLVITDHSMPKITGLEIARSLRENGI
                     DIPVILTSGYLAHTMGDEIRDVGVSEVLTKPITTQQLTETIGRVLGDPRPIDPS"
     gene            1945..>2311
                     /locus_tag="PKJ17_08120"
     CDS             1945..>2311
                     /locus_tag="PKJ17_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75978.1"
                     /translation="MRRIGTLAFGIFLLIFLAVVSAWAEDQKGERGADRKDGKKLVLD
                     KITVTETRYDNPVTPIDTRYGTQYNVVTEGQIEEQNSYDLQSTLRDVPGVSLYGAGGV
                     SSMPAIHGLADDRIRIKVDD"
BASE COUNT          554 a          694 c          663 g          400 t
ORIGIN      
        1 ggttgacgac catgcagctc acgggctgga cagtcgtctt tgcccagaac agggacgagg
       61 tgatggcccc cgtcaacagg atcctgtgga tcatgcttgt cagcggaata ctcttcctta
      121 tcatgacgat cacgacgata gtcatcgttt cgagcaggct cagttctccc gtgcagaggc
      181 ttctcgaaag gatgaagaag atgacggaac actccacgga gatcatcctc cagataggga
      241 ccgacaggaa gatcgccttt gcgaacccgg ccttcgaaag gataacgggg acaaggccgg
      301 aggaggtgat cggcaccgaa ccggacttcg ccaatcccca caatatcccg ccggacgtta
      361 tctgggactc cctcaattca ggaactccct ggtcgggaag ggtgtccctc cgggcaattg
      421 acgggagccc ggtcactctc gacgtgatgg tcatgcccct tagaaactca aacggcacag
      481 tggagggcta cctggaaata ggccgggacg tgacaaagga gctcatgttc gagaacaaga
      541 tgcagcaatc gcaaaagctt gaggccatcg gcactctggc cggtggaata gcccacgact
      601 tcaacaacat cctgagtggc atcctcggtt acgcggaact tgccctgatc acgggaaaaa
      661 atgatgggga gacagaaaag ttcctgaagg agattataaa agccgctcag agggcgggcg
      721 agttggtagg acagatactg accttcagca ggcagacgga ggtcgagctg agaccgatcc
      781 tgcccggacc catccttgag gaggcctcga agctcctgcg cgcgtccgca ccggcctcca
      841 tcgacatccg gttgcggatc gacagccgat ctgccatcct ggcggaaccg acccagatcc
      901 accagatcgt gatgaacctt tttacgaacg ccgtgcacgc cattgaaccg gaacacggaa
      961 ccatcaccat tgaactcgaa gatctcgtgg tcgatgacga cctcgcgaag gcccatcccg
     1021 acatgcgacc gggaagacac atcgcgctat ccgtctccga cacgggaagc ggcatggaac
     1081 aggagatcgt ggatcgtata ttcgagccct tcttcaccac gagatcccag gggaaaggca
     1141 ccggcctcgg gctctctgtc gtgcacggca tcgtgaagga actgggcggg atgatcgagc
     1201 tcaaaagcca ggccggcaag ggcacgacct tcagcatcta catcccctgt acggacatgg
     1261 acgctgccgg cccaccccgg gaagcccccc cgaccgagaa ggggagatac cggatcgtcc
     1321 tcatagacga cgaggaggca attcttacga ccacgggcgc catcctgacg cagctcggcc
     1381 acaaggtcac cacctttgcc aacggcctcg atgccctgga agcagtaaaa gaggaccccc
     1441 acgacttcga cctcgtgatc acagaccatt caatgccaaa gatcacgggt ctcgaaatag
     1501 cacggagtct tagagagaat ggcatcgaca tccccgtcat actcacctcg ggctacctcg
     1561 cccacaccat gggcgacgag attcgcgacg ttggcgtatc ggaagtgctg acaaaaccga
     1621 taacaacgca acagcttacc gagacgatag gcagggtgct tggcgacccc cggcccatcg
     1681 acccaagcta gggccccctc ccccattttt tcgatccttc catgatggaa aacatttctg
     1741 ttgacacgag gtgcagtgga tgctaccttt ttttcattag tagcacgcta gctgctttca
     1801 ggccctcccg accgacggat cgtgccgggg gggcggaagg gcgcctttcg acacagggca
     1861 tgaaagaata tgatcgcttc caccgccgag acccgccatt ccacgcggaa gacagggtga
     1921 tgcgatggac ccggagacca tgcgatgagg agaatcggaa cacttgcctt tggtatcttc
     1981 ctgctcattt tccttgcagt cgtgtcggcg tgggccgaag accagaaagg ggagcgcggg
     2041 gcggaccgga aggacggcaa gaagcttgtc ctcgacaaga tcaccgtaac ggagacgcgc
     2101 tatgacaacc ccgtgacgcc catcgatacc cgctacggca cgcaatacaa cgtggtgacc
     2161 gaggggcaga tcgaggagca gaactcctac gaccttcagt cgacgctgcg cgacgtgccg
     2221 ggcgtcagcc tttacggtgc cggcggcgtc tccagtatgc cggccattca cggcctggcc
     2281 gacgaccgca tccggatcaa ggtcgacgac a
//
LOCUS       DAOPOA010000381         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_50573_length_2306_cov_3.380720, whole genome shotgun sequence.
ACCESSION   DAOPOA010000381 DAOPOA010000000
VERSION     DAOPOA010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50573_length_2306_cov_3.380720"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="PKJ17_08125"
     CDS             complement(<1..1448)
                     /locus_tag="PKJ17_08125"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOD75979.1"
                     /translation="MKATAALLSEGATVPFIARYRKEVTGSLDEVAITAVRDRLEQLD
                     ELDKRRDSILSSLDERGLLTDELKDQLEKAKTLTVLEDLYLPFRPKRRTRATIAKEKG
                     LELLARCLFEVLPGFDPMQKVLEFVDSEKGVRSAEDALAGARDIIAEWVSEDADARAA
                     LREMLLTRGKITSRVVTGKEEEGTKFNDYYAWEEPVKAAPSHRILALRRGEREGFLIL
                     HITGCDDEALRWLKTHFIKKDTPAPGQVELAVTDSYDRLLLPSLETDVRLALKTRADE
                     EAIKVFADNLRQLLLASPLGEKSVMAIDPGFRTGCKVACLDRQGKLLRFETIYPHSSE
                     KQSAEAGRTIIELCEACAVDAIAVGNGTAGRETEAFLKGLQLPDRIPVVSVNESGASI
                     YSASDVAREEFPDHDVTARGAVSIGRRLMDPLAELVKIDPKSIGVGQYQHDVNQVALK
                     KGLDDVVMSCVNGVGVELNTASPQLLSYVSGLG"
     gene            complement(2116..>2306)
                     /locus_tag="PKJ17_08130"
     CDS             complement(2116..>2306)
                     /locus_tag="PKJ17_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOD75980.1"
                     /translation="NDFRKTNYGGPCPPSGTHRYFFKLYALDTKLDLSSNAKKQDLEK
                     AMKGHILGEAQTMGLYKR"
BASE COUNT          477 a          677 c          581 g          571 t
ORIGIN      
        1 ccgagaccgg acacataact caaaagctgg gggcttgccg tattgagctc aactccgacg
       61 ccgttgacac agctcatcac cacatcgtcc aggccctttt tcagggctac ctgattcacg
      121 tcatgctgat attgtcctac tccgatggat ttcgggtcga tcttgacgag ctccgccagc
      181 gggtccatga ggcgcctgcc tattgaaact gcaccgcgtg cagtcacatc gtgatcgggg
      241 aactcttcgc gtgcgacgtc cgaggcggaa tagatggaag ccccgctctc attcacgctg
      301 acgaccggta tgcggtcagg aagctgaagc cccttaagga atgcctccgt ctcccgcccg
      361 gccgtcccgt ttccaacggc tatggcgtcg accgcacagg cttcgcagag ttcgattatc
      421 gtccggccgg cttcggccga ctgcttctcc gacgaatgcg gatagatggt ttcaaagcgc
      481 aaaagcttcc cctgccggtc gaggcaagcg accttgcagc ccgtcctgaa accgggatcg
      541 atggccatga cgctcttttc gcccagaggc gacgcgagga ggagctggcg caggttgtcg
      601 gcaaatacct ttatcgcttc ctcgtccgcc cgtgtcttca gggcaaggcg cacgtcggtt
      661 tccagtgaag gaaggaggag ccggtcataa ctgtccgtaa ccgccagttc cacctggccc
      721 ggggcgggcg tgtccttctt gataaaatga gttttcagcc agcgcaaggc ctcgtcgtcg
      781 catccagtta tatgcaggat gaggaaccct tccctttcac cccgtcgcaa tgcaagtata
      841 cggtgagagg gcgccgcttt gactggttct tcccatgcgt agtaatcgtt aaatttggtg
      901 ccttcttctt ctttgcctgt tacaacccgt gacgttatct ttcccctggt gaggagcatt
      961 tccctgagcg ctgcccgggc atcggcatcc tcactcaccc attccgcaat aatgtcccgc
     1021 gcgcccgcga gggcgtcttc cgccgatctc acgccttttt cgctgtcgac gaattcaagc
     1081 accttttgca tgggatcgaa gccgggcaac acctcgaaaa ggcaccgcgc cagcaactcc
     1141 agccctttct ccttcgctat cgtcgcgcgc gtcctacgct ttggacggaa gggcagatag
     1201 aggtcttcga ggacggtcaa ggtcttggct ttctcgagct ggtctttgag ctcatccgtc
     1261 aacaatcccc gctcgtccag cgaactcagg atggagtccc gccgtttatc cagttcgtct
     1321 agctgttcga gccgatcccg gacggccgtt atcgcaacct cgtccaggga ccccgtcact
     1381 tctttccggt accgtgcaat gaacggtacc gtcgcccctt cggataaaag agctgcagtc
     1441 gctttcaccc ggtccggagc gatggacagc tcgacagcaa ttttatttat gtgatgtatg
     1501 ttcatggtat ttgtggaatc gtgaagagtg tggagtgagg tgagaagaga tcattctgac
     1561 ttctcgatgt aaggcaaaaa agggccgatg gtctggcctc ccaagacgat agtaatcaag
     1621 tattcaagct tcagagaaaa acaaatactt caagagttgt tcttctcctt actgtttact
     1681 ccttactccc cactccttac ggtctctatt agctttccag aatgaaaatg acttcgaggg
     1741 ttacgcgaaa ttctacaatt ttcccatctt cgacgcgtgc gcgctgttct ttgacgcgca
     1801 ggccggttat cccgcgaagc gtcttggacg cgcgtctgaa acctaccaca attgcatctt
     1861 cgaagctttt cggagacccg gcaatgacct ccgttatacg tgctaccctt ccgtcactcc
     1921 tcatacaacc tcccgatgct tttctatcaa ggttatcagg gcagatatgt agtgtcaaca
     1981 gctttttcga tccaggggcg gttcgaggtt ctgggttcga ggttctgggt taataagaac
     2041 aagatctgag cctatacaca tataatgaga acaacccata acctagaacc tcgaaccgcg
     2101 cccagaacac ccatattacc gtttatacag tcccatcgtc tgggcctcac caagtatatg
     2161 ccccttcatt gccttttcga ggtcctgttt cttcgcattg ctgctcaggt cgagcttcgt
     2221 atcgagggca tagagcttga agaaatacct gtgagtgccc gacggcggac agggcccccc
     2281 gtaatttgtc ttcctgaaat catttt
//
LOCUS       DAOPOA010000382         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_50609_length_2305_cov_2.747556, whole genome shotgun sequence.
ACCESSION   DAOPOA010000382 DAOPOA010000000
VERSION     DAOPOA010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50609_length_2305_cov_2.747556"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKJ17_08135"
     CDS             complement(<1..333)
                     /locus_tag="PKJ17_08135"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD75981.1"
                     /translation="MRIPVLLVSFMLLFCTQVHSAAHSDSVYLFLRGYFSEALGRYDD
                     AEANYSKALREDPGSAELRTSLASLFVKKGELQKAEELLDEAIAIAPTNRVALLILAG
                     IHASKGVTY"
     gene            complement(392..1021)
                     /gene="lepB"
                     /locus_tag="PKJ17_08140"
     CDS             complement(392..1021)
                     /gene="lepB"
                     /locus_tag="PKJ17_08140"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOD75982.1"
                     /translation="MEKKKTKTREYIESILIAALIALLVRSFVIQAYKIPSGSMEPTL
                     LVGDHLLVNRMSYVVKMPFVDNVLFTTGKPKRGDIIVFRYPEDPTKDYIKRVIATGGE
                     TVEIRNKTIFIDGKKIKDPWGHFRPEPASRGFLPFIDKDNIPPVKVPQDSYFVMGDNR
                     DNSLDSRYWGFVEKRHLVGKALIIYFSWDSGAMNALQYVRWSRIGWLIK"
     gene            complement(1082..>2305)
                     /gene="argS"
                     /locus_tag="PKJ17_08145"
     CDS             complement(1082..>2305)
                     /gene="argS"
                     /locus_tag="PKJ17_08145"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOD75983.1"
                     /translation="GDVLANLLEKTGHVVGREYYINDAGRQIRTLGRSTFLRLRELKG
                     ETVDYPKDLYQGDYVRELAQGVIDEGLSVPGGEEEAVDFLADYAGSRIMQGIKDDLGK
                     FGVHFDNFFLESSLYASGIVDETLDILKEQGIAFERDGALWFKTSLFEKDEDRVLIKS
                     DGQNTYFTSDIAYHLDKFKRSYDVLVDIWGSDHHGYIPRLKASVQALGRNKEDLRFLL
                     IQFVTLIKEGKPVGMSTRSGEFTTLREVLDEVGRDACRFFFLMRKSDAHLEFDLDLAK
                     KTSNENPVYYIQYAHARIESIFRNAREAGIDTDNLAGADCSLLIVKEELDLIRWITRY
                     HSVLEGSARSLEPHRVTFYLMELVGRFHSYYNRTRVLGSDAAVTRARLLLLSTLRGVI
                     RDGLGLVGVSAPEKM"
BASE COUNT          484 a          648 c          650 g          523 t
ORIGIN      
        1 gtaggtgacg cccttcgacg catggatgcc cgcaagtatc aggagggcca cccggttcgt
       61 gggggcgatg gcgatcgcct catcgaggag ttcctccgct ttctgaagct cccctttctt
      121 cacgaagagg gaggcaaggg aagtcctcag ctccgcggag ccggggtctt ctctcagggc
      181 cttgctgtaa ttcgcctcgg cgtcatcgta cctccccagg gcctcgctga aataccccct
      241 gagaaagagg tagacggaat ccgaatgagc tgctgaatgg acctgggtac agaacagaag
      301 catgaatgag acaaggagta ccggtattct catcaccctg aataatacca cagaatcgat
      361 gggaaatcta atcataggac ggcatgggag cctactttat gagccagccg atgcgcgacc
      421 accgaacgta ctgaagagcg ttcatcgcac cgctgtccca ggagaagtag attatgagcg
      481 ctttccccac caggtgccgt ttttccacaa aaccccagta gcggctgtcg agactgttgt
      541 ccctgttgtc gcccatgacg aaatagctgt cctgaggcac cttgacggga gggatgttgt
      601 ccttgtcgat gaagggcagg aaaccccgcg aggccggttc cggtctgaag tgcccccagg
      661 ggtcctttat cttctttccg tcgatgaata tggtcttgtt ccttatctcc accgtctcgc
      721 ccccggtggc tatgacgcgc ttgatatagt ccttggtggg gtcctcggga taccggaaga
      781 cgatgatgtc cccgcgcttc ggtttccccg tggtgaagag gacattgtcg acgaagggca
      841 tcttgaccac gtagctcatc ctgttgacga gcaggtggtc cccgacgagc agggtgggct
      901 ccatggaacc cgagggtatc ttgtatgcct gaatgacgaa actcctcacg agaagggcga
      961 tgagcgcggc tatgaggatc gattcaatgt actctctggt cttggtcttc ttcttttcca
     1021 ttaaagctcc ttattcatct ctccgccagg cggtaaggcg tgtgacgacc ggggcgggcc
     1081 cttacatctt ctcgggggcc gacacgccga cgagaccgag gccgtccctg atcaccccgc
     1141 gaagggtgga tagcaggaga agacgagccc gggtgaccgc ggcatcgctg ccaaggactc
     1201 tcgtcctgtt atagtaactg tgaaacctgc ccacgagttc catcaggtag aaagtgacac
     1261 ggtggggctc gaggctcctg gcgctgcctt cgaggacact gtgatagcgg gttatccacc
     1321 gtatgaggtc aagctcttcc ttcacgataa gcaggctgca atcggcgccg gcaaggttgt
     1381 cagtgtcgat cccggcctca cgggcgttcc ggaagatgct ctctatcctc gcgtgggcat
     1441 actggatgta atagacggga ttctcgttgg atgtcttctt cgcgaggtcc agatcgaact
     1501 caaggtgggc atcgctcttg cgcatgagaa agaagaaacg gcaggcatcg cggccgacct
     1561 cgtcgagcac ttccctgagg gttgtgaact cgccggaacg cgtcgacatt cccaccggtt
     1621 tgccttcctt tataagggta acgaactgta tgagaaggaa tctcaggtct tctttgttcc
     1681 tccccagggc ctgaaccgat gccttcaggc gggggatata cccatggtgg tctgaacccc
     1741 atatatcgac aaggacgtcg taggaacgct tgaacttgtc caggtgatag gcgatgtcgg
     1801 atgtgaagta ggtattctgc ccgtcggact tgatgaggac cctgtcctcg tccttctcga
     1861 aaagactggt cttgaaccac agcgcgccgt ccctttcaaa ggcaattcct tgttccttga
     1921 gtatgtccag ggtttcatcg acaatacccg aggcatacag agacgactcg agaaagaaat
     1981 tatcgaaatg tacaccaaac tttccgagat catcctttat tccctgcatt atgcgggaac
     2041 ccgcgtagtc tgccagaaaa tcgaccgcct cctcctctcc gccgggaacg gaaagccctt
     2101 catcgatgac cccctgcgcc agttcgcgaa cgtagtcgcc ctggtaaagg tccttgggat
     2161 aatccaccgt ttcgccttta agttccctga gcctgaggaa ggtggaccgg ccaagcgttc
     2221 tgatctgccg gcccgcatcg ttgatgtagt attccctgcc cacgacatgc cctgtcttct
     2281 cgaggaggtt ggcaaggacg tcccc
//
LOCUS       DAOPOA010000383         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_50687_length_2302_cov_3.459279, whole genome shotgun sequence.
ACCESSION   DAOPOA010000383 DAOPOA010000000
VERSION     DAOPOA010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50687_length_2302_cov_3.459279"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /gene="cooS"
                     /locus_tag="PKJ17_08150"
     CDS             complement(<1..1778)
                     /gene="cooS"
                     /locus_tag="PKJ17_08150"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HOD75984.1"
                     /translation="MADGKEKSIDKATVELIDKAQREDISTVFSRADTLKPCPIGVEE
                     SCCKICSMGPCRMPRSRKGEGTRRVGVCGATVDTVVARNFARKVAAGSAAHSDHAREV
                     VITFLKTARGESQGFSIKDEIKLLEVALDFGINIEKRDVKDIAIELGEKALNEFGKQS
                     GELIYAGKAPLRRQEIWRKLGVLPRGIDREIVEVMHRTHMGVDQDYRHILQQAARAAI
                     ADGWGGSMISTELQDIMFGTPVPVLGSINLGVLKEDHVNVVVHGHEPLLPEMLVAASR
                     DPGIRELTEKVGAKGVNLAGMCCSANEILMRHGIPCAGNFLQQELALVTGAVELMTVD
                     VQCQMQGLASVAECFHTKLITTSDRAMIEGAEHVEFHPEQGLDIAKRILAMAIENYPN
                     RRHAVYIPAESQDMVAGFSHETITYLLGGLFRASYRPLNDNIANGRIRGVAGVVGCNN
                     VRTQHDAAHLAMIRELIRNDVLVLTTGCSAMACGKAGLLTPEAAHLHAGGGLAEVCEA
                     IGIPPVLHLGSCVDNSRILIAATAMVKEGGLGDDLSDLPAAGAAPEWMSEKAISIGQY
                     FVASGIFTVFGTTWPTLGSENVTEHL"
     gene            complement(1797..>2302)
                     /locus_tag="PKJ17_08155"
     CDS             complement(1797..>2302)
                     /locus_tag="PKJ17_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75985.1"
                     /translation="CEGKNVAVGPVSDKNYKQIGAMAIGYSQVVMASSPIDVNLAKQL
                     NILLGNLGVNEEKVVIDPTTGGLGYGLEYTYSVMERDRMAALTQEDAKLQFPIICNLA
                     PEVWKTRETRLTKDEDPKLGDEKRRSILMEAVTAMTLLVAGADIVVMRHPDSVFLVRE
                     MIGELCA"
BASE COUNT          403 a          732 c          673 g          494 t
ORIGIN      
        1 aagaggtgct ccgtaacgtt ctcgctcccc agcgtgggcc aggtggtgcc aaaaacagtg
       61 aagatccctg aggcgacgaa gtactgaccg atggagatgg ccttttcgct catccactcc
      121 ggcgcagcgc cggcggccgg caggtcggac aggtcatcgc cgagcccgcc ttctttgacc
      181 atggccgtcg cggcgatgag tatcctggaa ttgtcgacac aggacccgag atgaaggacg
      241 ggagggatac cgatggcctc acagacctcc gccagtcctc cgccagcgtg gagatgagcc
      301 gcctccgggg taaggagccc ggccttcccg caggccatgg cgctgcaacc cgtcgtcagg
      361 acgaggacat cattcctgat gagttcccgt atcatggcaa gatgggctgc atcgtgctgt
      421 gttctcacat tgttgcaccc gacgacaccc gcgacacccc tgatcctgcc gttggcgata
      481 ttatcgttga gcggcctgta gcttgcccgg aagagaccgc ccagcaggta ggttatcgtc
      541 tcgtggctga aacccgccac catgtcctga ctctcggccg gaatgtacac cgcgtggcgc
      601 ctgttgggat agttctctat ggccatggcg agtatcctct tcgcgatatc gagcccctgt
      661 tcgggatgga actcgacatg ttcggcgccc tcgatcatgg ccctgtcgga cgtggtgatc
      721 agtttcgtgt gaaagcattc cgccacgctc gccagcccct gcatctggca ttggacgtcg
      781 acggtcatga gctcgacggc gcccgtgacg agcgccagct cctgctgcag aaagttgccg
      841 gcacagggaa tgccgtgacg catgagtatc tcgttcgcgc tgcagcacat gcccgccagg
      901 ttcacaccct ttgccccgac cttttccgta agctccctga tgccggggtc gcggctggcg
      961 gcaacgagca tctcgggcag gagcggctcg tggccgtgga ccacgacatt gacgtggtct
     1021 tccttgagaa cgccgagatt gatcgagccg agcacgggca ccggggtgcc gaacatgatg
     1081 tcctgcagct ccgtggatat catcgagccg ccccagccat cagcgatcgc cgcgcgcgcg
     1141 gcctgctgga gaatgtgccg gtaatcctga tcgacaccca tgtgggtcct gtgcatcacc
     1201 tccactatct cgcgatctat gccgcgcggc aggaccccga gcttcctcca gatctcctgc
     1261 ctgcgcaggg gcgcctttcc cgcgtagatg agttcgccgc tctgtttgcc gaactcgttc
     1321 agcgcctttt cgccgagctc tatggctatg tcctttacgt cacgcttctc tatattgata
     1381 ccgaaatcga gagcgacctc gagaagcttt atctcgtcct tgatggagaa accctgcgat
     1441 tcacccctgg ccgtcttgag aaaggtgatg acgacctcac gggcgtggtc cgagtgggca
     1501 gccgaaccgg ccgcgacctt cctggcaaag ttccgggcca ccacggtatc cacggtcgca
     1561 ccgcagacac ccacgcgccg cgtgccctca cctttccttg agcgcggcat tctgcaaggg
     1621 cccatggagc agatcttgca acagctctct tccaccccga tgggacaggg tttcagcgtg
     1681 tcggcccggg agaaaacggt ggagatatct tcccgctgag ccttgtcgat aagctcaacg
     1741 gtcgccttgt caatgctctt ttccttgccg tcggccattg tctctccttc tacttgtcac
     1801 gcacagagtt cgcctatcat ctcccggaca aggaatacgc tgtcgggatg gcgcataacg
     1861 acgatgtccg cccccgctac aaggagcgtc attgcggtga ccgcctccat gaggatggag
     1921 cgcctcttct cgtctcccag cttcgggtct tcgtccttcg tcagccttgt ctcccgggtc
     1981 ttccagacct ccggggcaag gttgcagatg atggggaact gaagcttggc gtcctcctgc
     2041 gtgagcgccg ccatcctgtc gcgttccatc accgaatagg tgtattcgag gccgtagccg
     2101 aggcctcccg tcgtgggatc gatgacgacc ttctcctcgt tgacgccgag gttgccgagg
     2161 aggatgttga gctgcttcgc cagattgacg tctatgggtg aggatgccat gacgacctgg
     2221 ctgtagccga tggccatggc gcctatctgt ttgtagttct tgtccgaaac gggaccgacg
     2281 gcaacatttt tgccctcgca gg
//
LOCUS       DAOPOA010000384         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_50820_length_2298_cov_3.079804, whole genome shotgun sequence.
ACCESSION   DAOPOA010000384 DAOPOA010000000
VERSION     DAOPOA010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50820_length_2298_cov_3.079804"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKJ17_08160"
     CDS             <1..475
                     /locus_tag="PKJ17_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOD75986.1"
                     /translation="GSLLTLSGFDVTILAVVTGVLVLFVAVFFKEIRAVLFNRRLARA
                     SGVPERMIYYALLFLTGAVVSSNLATVGGLLIFALLVQPGATALQLTYNMKHFFLISA
                     ASGIGACASGLVISYIFDLPSGASVVLMATVIFAAAYLFSPKRRRARLTERNRII"
     gene            472..1125
                     /locus_tag="PKJ17_08165"
     CDS             472..1125
                     /locus_tag="PKJ17_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD75987.1"
                     /translation="MKMTDDERYARRREFFNEHAEKWLDMWYKDRVTGIYDKHAKDFE
                     RLFSLMPLRPGDHVLDVGCGSGILVPFILERITSTGLLYELDFAEKMLEVNRGLHEEG
                     NIRFILADAENAPLADNSCDVVVCFSCFPHFHDKEKALATLCRILKPGGHLIVSHFSS
                     AEGINKHHQSCHAVMHDHLPDEGSMRGLLTNAGLTITDFMDEKSFYYISSTLSGPAF"
     gene            complement(1251..>2298)
                     /locus_tag="PKJ17_08170"
     CDS             complement(1251..>2298)
                     /locus_tag="PKJ17_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD75988.1"
                     /translation="ILSRDEFGRPMENPPVLWDIESDPILAGVKLIAEAWDAAGLYQV
                     GRFIGDNWKEWNGKFRDDVRAFLRGEEGTVTRLAQRLMGSPDIYGGREREPEQSVNFV
                     TCHDGFSLNDLVSYNHKHNETNGEENRDGTDYNLSWNCGFEGPTDDPAIDGLRNRQIK
                     NFITITLISVGTPMLSMGDEVRLTRYGNNNSYCQDNETSWFDWSLLTRHADMLRFTKS
                     LIALRLSRRYAKAERSMTLNELLRKARMSWHGVKLGEPDWADHSHSLAVTLEVEKGLL
                     FHIMLSSYWGPLEFELPPTGSARGSWRRLIDTSLESPDDFCAWNLAPRIAGDEYQLQS
                     YSVAILFAHATEEV"
BASE COUNT          429 a          720 c          666 g          483 t
ORIGIN      
        1 gggaagtctt ctgacgcttt ccggcttcga cgtcacgata ctggccgtgg tcacaggcgt
       61 cctcgtcctc ttcgttgccg tcttcttcaa ggagattcgc gcggtcctct tcaaccggcg
      121 gctcgccagg gcgagcgggg tgccggaaag gatgatctac tacgcgctcc ttttcctcac
      181 gggcgccgtc gtatcctcca acctcgcaac cgtcgggggc cttctcatct tcgccctcct
      241 ggtgcagccc ggggcgacgg cactccagct cacgtacaac atgaagcact tcttcctcat
      301 ctcggcggcc tccgggatcg gcgcctgtgc ctcaggcctc gtcatctcct acatcttcga
      361 cctgccatcc ggagcctcgg tggtcctcat ggccacggtc atcttcgccg ccgcgtacct
      421 cttttcacca aaacgccgca gggccagact cacggaaagg aaccgcataa tatgaaaatg
      481 acagacgacg aacgatatgc caggcgcagg gaattcttca acgagcacgc ggagaagtgg
      541 ctcgacatgt ggtataagga ccgcgtcacg gggatctatg acaaacacgc caaagacttc
      601 gaacgcctct tctcgctcat gccgctaaga ccgggagacc acgtgctgga cgtggggtgc
      661 ggaagcggca ttctcgttcc cttcatcctc gaacgcatca cgtcgacggg acttctctac
      721 gaactcgatt tcgcggaaaa gatgctggag gtcaaccgcg gcctccacga ggagggaaac
      781 atcaggttca tcctggcgga cgcagagaac gcgccgctcg ccgacaactc ctgcgacgtc
      841 gtcgtctgct tctcctgctt cccccatttc cacgataagg aaaaggccct ggcgacgctc
      901 tgccgtatct tgaagcctgg cggtcacctc atcgtatccc atttcagctc tgcagagggg
      961 atcaacaaac accaccaatc ctgtcacgcc gtgatgcacg accatctccc cgacgaaggg
     1021 tcgatgcgcg gcctgctcac caacgcgggc ctcaccatca cagacttcat ggacgaaaaa
     1081 agcttctact atatctcttc caccttatcc ggccccgcgt tctgacatat gtcatcggca
     1141 acgggcggac gggacggagt gtctgcatca ttttggtcat tggtcattgt ctgtttatct
     1201 ggtcattgga atttggtcaa ttggtcatta tcctcgacag ccgtgatcca tcacacctct
     1261 tctgtcgcat gagcgaagag gatcgccacg gaataagatt gcagctggta ttcgtctccc
     1321 gctatccgag gggccaggtt ccaggcgcag aagtcgtcgg gggactcgag ggacgtgtcg
     1381 atcagtcggc gccaggatcc ccgggctgac ccggtcggcg gcagctcgaa ctcgagaggc
     1441 ccccagtagc tgctgagcat tatgtggaac aggaggcctt tctcgacctc gagggtcacc
     1501 gcgaggctgt gcgagtggtc ggcccagtcg ggctcgccga gcttcacccc gtgccacgac
     1561 atgcgggcct tgcgaaggag ttcgttgagg gtcatcgacc gctcggcctt tgcgtaccgc
     1621 cgggaaagcc gaagcgcgat gaggctcttg gtgaagcgga gcatgtccgc gtggcgggtg
     1681 agaagagacc agtcgaacca gctcgtctcg ttgtcctgac agtagctgtt gttgttcccg
     1741 taccgggtga gccgcacctc gtcgcccatg gagagcatcg gcgtgcccac ggagataagg
     1801 gtgatggtga tgaagttctt gatctggcgg ttccggagcc catcgatggc agggtcgtca
     1861 gtgggaccct cgaaaccaca gttccagctc aggttgtagt ccgtgccgtc cctgttttct
     1921 tctccgttcg tttcgttgtg cttgtggtta taggagacga ggtcattgag ggaaaacccg
     1981 tcatggcagg tcacgaagtt cacgctctgt tcgggctcgc gctcccgccc gccgtagata
     2041 tcggggcttc ccatcaggcg ctgggcaagc cgtgtcacgg tcccctcttc gcccctgaga
     2101 aaggcccgca cgtcatcccg gaacttgccg ttccattcct tccaattgtc gccgataaag
     2161 cggccgacct ggtacagccc cgccgcgtcc cacgcctcgg cgatgagctt gaccccggcg
     2221 aggatgggat cggactcgat atcccacagc acgggcgggt tctccatggg ccggccgaac
     2281 tcgtcgcggg agaggatg
//
LOCUS       DAOPOA010000385         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_51032_length_2289_cov_3.066697, whole genome shotgun sequence.
ACCESSION   DAOPOA010000385 DAOPOA010000000
VERSION     DAOPOA010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51032_length_2289_cov_3.066697"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..652
                     /locus_tag="PKJ17_08175"
     CDS             68..652
                     /locus_tag="PKJ17_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOD75989.1"
                     /translation="MKKVSFAMAVFLAFLCIGSFVWPRAAAGAGSTDMFLLAFGKGKT
                     EIKLYSDYFCGACKNLEPNVEYLISDLVARDVVTITFVDTPMHRLSTLYARYFLYILN
                     AKKEIRHALKARRALFEAAQKNISDSGKLEDFLASKGLKWKPFDVAPVFSVLQGYLRS
                     DTINATPTAVTLRDGARKVHQGVPNITRMLEGLK"
     gene            668..1108
                     /locus_tag="PKJ17_08180"
     CDS             668..1108
                     /locus_tag="PKJ17_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOD75990.1"
                     /translation="MNERDEEKKRLLETARVIAVVGLSPDTTKASNIVAQYLMTHGYR
                     VIPVNPEYTEILGQRSYPSLTSVPERIDIVDVFRKAGDALPVVEEAVTLAPKAIWLQL
                     GIVNDEAARIARDRGITFLMDLCIKQEHARLIGKEKKRGPLGAS"
     gene            1175..>2289
                     /locus_tag="PKJ17_08185"
     CDS             1175..>2289
                     /locus_tag="PKJ17_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOD75991.1"
                     /translation="MKRTLSQHTGSHEEGADLFLKGGTILNVYSGELLEGNIAVRGER
                     IVYVGPSEHHVGKDTRVLELVGKTITPGYIEPHFHPWDVYNPVSVGEEACRRGTTTVF
                     CDDLVFYMEMGPVLFQEFMDAFSDMPIKYYWSLRTAPQSPMRDEKEIFSVENLLAVLE
                     NPRVQSIGEITRWMELIEGTSRIPPVIARTKELKKRVDGHTAGARYDKLNRLALAGIE
                     SCHESINGQNVLDRLRLGFYVMLRESSLRQDLSLLLKTVARERVLTDRLLLTTDGSMP
                     EYYHRQGVTDHLIRIALKEGIDPVSAYRMATINPAVYFGFDHRIGGIAPGRDADMVIL
                     KDIHEPTPETVISRGRIVAREGTLIQSFPAMDWERYFP"
BASE COUNT          547 a          688 c          630 g          424 t
ORIGIN      
        1 ctgaccgggc accccccgag ctttttctct ttacaagggg gcaaccgctg gaccatactt
       61 taagaccatg aagaaggtct cattcgccat ggcggtcttt ctcgcgttcc tgtgcatcgg
      121 ctcctttgtg tggccccgag ccgccgccgg cgccgggtcc acggatatgt tcctcctcgc
      181 cttcggcaag ggaaagaccg aaatcaagct ctattccgac tatttctgcg gggcctgcaa
      241 gaacctcgaa cccaacgtcg aatacctgat ttccgatctc gtggcgagag atgtcgtcac
      301 catcaccttc gttgacaccc ccatgcacag actgtccacc ctctacgcac gctatttcct
      361 ctacatcctc aacgcgaaga aggagatacg ccacgccctc aaggcgagaa gggccctctt
      421 cgaggcggca caaaaaaata tctccgacag cgggaagctg gaggatttcc tcgcctcgaa
      481 aggcttgaag tggaaacctt tcgacgtggc gcccgtcttc tccgttctcc agggatacct
      541 tcgcagcgac accataaacg ccactccgac ggccgtcacc cttcgggacg gtgcgagaaa
      601 agtccaccag ggtgttccga acataacacg aatgcttgaa ggactgaaat agaccccgga
      661 gcgagccatg aacgaaagag atgaggagaa gaaaaggctt cttgaaacag cgcgggtgat
      721 agcagtggtc ggtctttcac ctgatacaac caaggcaagc aacattgtcg cgcaatactt
      781 gatgacccac ggctaccggg tgatacccgt taacccggaa tacacggaga tactgggaca
      841 gaggtcctat ccctccctca cgagcgtgcc cgaaaggata gatatagtcg acgtcttccg
      901 aaaggccggc gatgccctgc ctgtcgtgga ggaagctgtg actctcgccc cgaaggcgat
      961 ctggctgcag cttggcatcg tgaacgacga agccgcgagg atcgcccggg acaggggaat
     1021 caccttttta atggacctct gcatcaagca ggaacatgca aggctcatcg ggaaagaaaa
     1081 gaagcgaggg cccttagggg cctcctgaca cacctcacgg caaccggcga ccggacagcg
     1141 agggtttgat gaaagatccc cggggaaaac tgcggtgaag agaacgcttt cacaacacac
     1201 agggtctcac gaagaagggg ccgacctttt tctcaagggc gggaccatac tcaacgtcta
     1261 ttccggggaa ctgctcgaag gcaacattgc cgtccgcggc gaaaggatcg tctacgtggg
     1321 tccttcggaa caccatgtcg gaaaggacac gagagtcctc gagcttgtgg gcaagacgat
     1381 aacccctgga tacattgaac cccatttcca tccctgggat gtgtacaatc ccgtgtccgt
     1441 cggtgaagaa gcctgcaggc ggggaacaac caccgtcttc tgcgacgacc ttgtgttcta
     1501 catggaaatg ggcccggtgc ttttccagga attcatggat gccttctcgg acatgcccat
     1561 caagtactac tggtcgttgc ggacagcgcc ccaatccccc atgcgggacg agaaggaaat
     1621 cttctccgtg gaaaacctcc tcgccgtcct cgagaacccc cgcgtccagt ccatcggtga
     1681 gatcacgcga tggatggaac tgatagaggg cacctcccgg ataccccccg tgatagcgag
     1741 gacgaaggag ctgaagaaaa gagtggacgg ccacacggca ggcgccaggt acgacaagct
     1801 caaccgcctt gccctggcgg gaatagaatc ctgtcacgaa agcatcaacg gccagaacgt
     1861 tctcgacagg ctgagactcg gattttacgt catgctccgc gaaagctccc tgaggcagga
     1921 cctcagtctc cttctcaaga ccgtcgccag ggagcgcgtc ctgacggacc gccttcttct
     1981 caccacagac ggctccatgc ccgagtacta tcacaggcag ggtgtcacgg accacctgat
     2041 acggatagca ctcaaggaag ggatcgatcc cgtgagcgct taccggatgg caacgatcaa
     2101 ccccgccgtc tacttcgggt tcgaccacag gatcggaggg atagccccgg gacgggacgc
     2161 cgacatggtg atcctcaagg atatccatga gcccaccccg gagaccgtta tttcccgcgg
     2221 caggatcgtc gcgcgagaag gcacgctcat ccagtccttc ccggcaatgg actgggaaag
     2281 gtattttcc
//
LOCUS       DAOPOA010000386         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_51086_length_2287_cov_3.804211, whole genome shotgun sequence.
ACCESSION   DAOPOA010000386 DAOPOA010000000
VERSION     DAOPOA010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51086_length_2287_cov_3.804211"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKJ17_08190"
     CDS             complement(<1..302)
                     /locus_tag="PKJ17_08190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD75992.1"
                     /translation="MEKPVDRKYPSVRAVSEAERIGMVKEMFSTITGKYDFLNHFLSL
                     RRDIAWRRFTVKKMRFFKTMRFLDVACGTADLSIDAAMKYGGISVTGADFVFEMLEL"
     gene            complement(287..1033)
                     /locus_tag="PKJ17_08195"
     CDS             complement(287..1033)
                     /locus_tag="PKJ17_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD75993.1"
                     /translation="MKRRAIGVYAATILIIYFAWYALSLMLGSNILPDPFRVFIRGFE
                     EIATTAFWVHVEASLLRLLAALFFAFILAVPAGLVLGSNERLDGLFAPLIYLGYPIPK
                     VVLMPVIFVLFGLGDLGKVVLITMIIFFQLLITTRDSARKIDREITYSLRSLGGGRLD
                     YFFHVVWPVSLPGIFTSLRIGVGTAVAVLFLVESIATSEGLGFYIIDSWGRAEYATMF
                     VGIIALSIIGIVMYEIFELLERKFCKWKNL"
     gene            complement(1030..1788)
                     /locus_tag="PKJ17_08200"
     CDS             complement(1030..1788)
                     /locus_tag="PKJ17_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD75994.1"
                     /translation="MGTSGKFLAIEGLGKTFQNENDSKTVLEGVDLELAFRDCLAIVG
                     PSGCGKTTLLLAIAGLIPPTAGAITFRDGPLAAPERRIALVLQHYGLFPWKTVGANIA
                     LGARLQHIDVPEKRLRSVKRELDIEGLDHLYPAQLSGGQRQRVALARALLLNPLLLLL
                     DEPFAAIDTVTRERLQNNLLDVFAQRRFSFIIVTHNIEEAVFLGRRIVVLDGNNRGIK
                     TIMDNPGMGDPQYRNDPVFFERTTELRGILEGLE"
     gene            complement(1772..>2287)
                     /locus_tag="PKJ17_08205"
     CDS             complement(1772..>2287)
                     /locus_tag="PKJ17_08205"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD75995.1"
                     /translation="AEYLIIRLLADKKVPRESIKLVDIKSIPIRLQMLLSGQVSAALL
                     PEPLATLAETKGAKTLADDRGHGISATVLAFHTDFLDKNAASVRSFLAAVDKASAYIS
                     RHPDEVRAIMNKSCKVPEALQSSFPIPRFPKVHAPSESQVMDVYRWLKEKKIVKKDLT
                     YKDLVADGYIR"
BASE COUNT          462 a          629 c          738 g          458 t
ORIGIN      
        1 agttccagca tctcgaaaac gaaatcggcg ccggtgacgc ttatcccgcc gtatttcatc
       61 gcagcgtcga tggaaaggtc ggctgtgccg caggcgacat cgagaaaccg catggtcttg
      121 aagaagcgca tcttcttcac ggtgaaacgg cgccacgcta tatcgcggcg caggctcagg
      181 aagtgattga gaaagtcgta cttgcccgtg atggtggaga acatctcctt caccatgccg
      241 atgcgctccg cttccgacac cgcgcgcacc gaagggtatt tcctgtctac aggtttttcc
      301 atttgcagaa tttcctttca agaagctcga atatctcgta catgacgatg ccgatgatcg
      361 agagggctat gatccccacg aacatggtgg catactcggc gcgtccccag gagtcaatga
      421 tgtagaatcc gaggccctcg ctcgttgcga tggattcgac gagaaagagg accgccaccg
      481 cggtccccac gccgatgcga agcgatgtga atatcccggg caggctgacc ggccagacga
      541 catggaagaa gtagtcgagc ctgcctcctc ccagcgaccg gagcgagtag gtgatctccc
      601 ggtcgatctt gcgcgcgctg tcacgggtgg tgataaggag ctggaagaat atgatcatgg
      661 tgatgaggac gactttgccc aggtccccga ggccgaaaag gacgaagatg acaggcatga
      721 ggaccacctt ggggatgggg tagccgaggt agatgagcgg cgcgaagagt ccgtcaagcc
      781 tttcgttgct cccgaggaca agacccgccg gcacggcgag gatgaaagcg aagaagagtg
      841 ccgcgaggag cctcaggagg ctcgcctcaa catggaccca gaaagcggtc gtcgctattt
      901 cctcgaagcc gcggatgaac acccggaaag ggtcggggag gatgttgctg ccgagcatga
      961 gggaaagggc gtaccatgcg aaatagatga taagaatggt ggcggcgtaa acgccgatgg
     1021 cccgcctctt cattcgagac cctccaggat gcccctgagc tcggtggtgc gctcgaagaa
     1081 gaccgggtcg ttgcggtatt gcgggtctcc catcccggga ttgtccatga tcgtctttat
     1141 cccgcggttg ttaccgtcga ggacgacgat ccttcttccc aggaataccg cctcttcgat
     1201 gttgtgcgtg acgataatga aactgaacct ccgctgcgcg aacacgtcga ggaggttgtt
     1261 ctggagccgc tcccgcgtga cggtgtcgat ggcggcaaag ggttcgtcga gaagaaggag
     1321 cagggggttg agaagcagcg cccttgccag cgcgacacgc tggcgctggc ctccgctgag
     1381 ctgcgccggg tagagatggt cgagaccctc gatatcgagt tccctcttga cggagcgcag
     1441 tctcttttcg ggcacgtcga tgtgctggag acgggccccg agagcgatgt tggcgcccac
     1501 ggtcttccac ggaaagagtc cgtaatgctg gaggacgagg gcgatcctgc gttccggggc
     1561 ggcgagcggt ccgtccctga aggtgatggc tcccgctgtc ggtgggatga gccccgcgat
     1621 ggcaagcaga agggtggtct tgccgcaccc cgaagggccg acgatggcaa ggcagtcgcg
     1681 aaaggccagt tcgaggtcga ccccctcaag gaccgtcttc gaatcattct cgttctgaaa
     1741 ggtcttgccc agtccctcga tggcgaggaa cttaccggat gtacccatct gccaccaggt
     1801 ctttgtacgt gaggtccttc tttacgatct tcttttcctt aagccaccgg tagacatcca
     1861 tgacctggga ctcggagggt gcgtgtactt tcgggaagcg cggtatgggg aaagagctct
     1921 ggagtgcctc cggtaccttg caggacttgt tcatgatcgc ccgcacttcg tcggggtgcc
     1981 ggcttatgta tgccgaggcc ttgtcgacgg ccgcgaggaa cgaccggacg gaagcggcgt
     2041 tcttgtcgag gaagtcggtg tgaaaggcga ggaccgtcgc cgagatgccg tggcccctgt
     2101 cgtcagccag ggttttcgct ccctttgttt ccgccagcgt cgccaggggc tccggcagca
     2161 gtgccgcgct tacctgtccc gaaaggagca tctggagacg gatgggtatg ctcttgatat
     2221 ccacgagttt gatggattcc cggggaacct tcttgtccgc caggaggcgg atgatgagat
     2281 attccgc
//
LOCUS       DAOPOA010000387         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_51177_length_2284_cov_4.930013, whole genome shotgun sequence.
ACCESSION   DAOPOA010000387 DAOPOA010000000
VERSION     DAOPOA010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51177_length_2284_cov_4.930013"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..2220
                     /locus_tag="PKJ17_08210"
     CDS             145..2220
                     /locus_tag="PKJ17_08210"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOD75996.1"
                     /translation="MRTTENLSKVGFLHFLFLACLALIVFVPVRVCSADLPYTATIAE
                     GRRAVKEIMERTGASSVSVAFTDGDRLVWTETFGYADRASGTPPTKETMYCIGSTSKM
                     FATVAVMKLVDRGLIALDEPVVKHLKSFRMLSPEYRQITVRMLLNHSSGFPGTDYRNA
                     FTTTPFKDYSAQVLETLATERLKHAPGYLSVYCNDGFTVAEQLIASVTGKSYPRFVED
                     EILKPLGMKSSRYMLDYPPEGSAAKRYDGKGRELPFMFVNVLASGGLYSTPSDMARFA
                     MMLIGKGRLGDARILSEKAVAEMAKDQTLASFNPVKSNMVSYGLGWDTVRQPGLGAVG
                     VTAWQKGGAIPFMKALFTVVPDERLAAFVVGASGNFSSNDTIVLMERLLLNALVEKGR
                     IPSVPSPLKGKALPEKTASPAELRSFAGCYGRSDSLLRVSGGRGQSLSIYRYDPEKKR
                     WGAWQKGLTMRSDGYFASKADPLQAFAFRTADGRQYLINRSVAGYGHYQDNEVIGQKV
                     TVTAALPASWKKRLARHWLMINDNPTSDAWEAPGADLGNYGGLLLSSDSQVVDPTLSD
                     SRAGMMLVIPQLKGRDLNDLVIENRAGDEWLRLGSYLFQPRETVEKLAPGETTIVLGP
                     DGNSEWRVIDRLNKAKTLTISPDLPDGSWRLYDARLKQIERGKGKKTLPLAGGTYYLL
                     FHDNARVLF"
BASE COUNT          480 a          662 c          673 g          469 t
ORIGIN      
        1 aagagacgcg ccccattgaa catggtctga aggtttcgtg ccttccggca gggctttttt
       61 cggattgacg agcctctacg cgtatcatat actcctcaat acctgacaaa tcctgagaat
      121 tcataaagat agggggacga gtagatgagg accaccgaaa acctgtcaaa ggttggtttt
      181 ctccatttcc tgtttcttgc gtgccttgcg ctgatcgtat tcgttcctgt tcgggtgtgt
      241 tccgcggacc ttccttacac ggccacgatc gccgaaggac ggagggcggt caaagaaatc
      301 atggagcgga ccggcgcgtc ttcggtctcc gtggccttta ccgatggtga caggcttgtc
      361 tggactgaga cctttggcta tgccgaccgg gcgtcgggga cgccgccaac gaaggaaaca
      421 atgtattgca tcggatcaac gagcaagatg ttcgccaccg ttgccgtcat gaaactcgtt
      481 gaccgggggc tgatcgcgct tgacgagccc gtcgtgaagc atctcaagtc tttcaggatg
      541 ctttctcccg aataccggca gatcacggtc cggatgctgc tcaatcattc gtcgggattt
      601 ccgggaaccg attatcgcaa tgcctttaca acgacgccct tcaaggacta ttcggcacag
      661 gttctcgaga cccttgccac cgagcggctc aagcacgcac cgggatatct cagcgtctac
      721 tgcaacgacg gtttcaccgt tgcagagcag ctcatcgcgt ctgtgacggg aaagagttat
      781 ccccggttcg ttgaagacga gatcttaaag cctctcggca tgaagagctc gaggtatatg
      841 ctcgactatc cccccgaagg gagcgccgcg aaacgctacg acgggaaagg cagggagctg
      901 cccttcatgt tcgtcaacgt tcttgcctcg ggtggcctgt actcaacgcc ctccgacatg
      961 gccagattcg ccatgatgct catcggaaag ggcaggcttg gcgacgcccg catcctgtcg
     1021 gaaaaggcag tcgccgaaat ggccaaagac cagaccctgg cgagcttcaa ccctgtcaaa
     1081 tccaatatgg tcagctatgg gctcggatgg gacacggtgc gccagccggg attgggggcc
     1141 gtcggcgtga cggcctggca gaagggcggg gccatcccgt tcatgaaggc gctcttcacc
     1201 gtcgttccgg acgagaggct ggcagccttc gtcgtcggtg catcggggaa tttcagctcc
     1261 aatgacacca tagtgctgat ggagagactc ctccttaacg ccctcgtcga gaagggccgc
     1321 atcccatcgg ttccttcccc tctgaaagga aaagccctgc cggagaagac ggcctcccct
     1381 gcggagctcc ggtccttcgc cggctgctac gggcgcagcg attccttgct gcgcgtgagc
     1441 ggaggtcgtg gtcaaagcct gagcatctac cgctacgatc ccgaaaagaa gagatggggc
     1501 gcctggcaga agggactcac gatgcgctct gacggttatt tcgcttcgaa ggccgatccc
     1561 ttgcaggcct ttgccttcag gaccgcggac ggacggcagt acctgatcaa ccgctctgtt
     1621 gccggatacg gccattacca ggacaatgag gtcattggcc agaaggtcac ggttaccgcg
     1681 gccctgccgg cctcgtggaa gaagcgtctg gcgcggcatt ggctcatgat caacgacaac
     1741 cccacttccg acgcatggga agcccccggc gccgaccttg ggaactacgg gggcctgctc
     1801 ctctcgagcg attcgcaggt cgtcgacccg accttgagcg attcccgggc cggcatgatg
     1861 ctcgttattc ctcaattgaa aggcagggac ctaaacgatc tcgtcatcga gaaccgggcc
     1921 ggcgacgaat ggctgcgcct gggcagttat cttttccagc cccgcgagac cgttgagaaa
     1981 ctcgccccgg gcgagacaac gatcgtgctc ggtcctgatg gaaatagtga gtggcgtgtc
     2041 atcgaccggc taaacaaggc gaagacactg accatctccc ctgacctgcc cgacggctcc
     2101 tggagactct acgatgccag gctgaaacag atcgagaggg gaaagggaaa gaagaccctg
     2161 cctcttgccg gcggtacgta ttatctgctc ttccacgata atgccagggt gcttttttga
     2221 aacgatccca tgtataaatg cggtaggtcg cgggggcgcc gaaagagcga atggtgaatc
     2281 gttg
//
LOCUS       DAOPOA010000388         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_51214_length_2283_cov_3.432675, whole genome shotgun sequence.
ACCESSION   DAOPOA010000388 DAOPOA010000000
VERSION     DAOPOA010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51214_length_2283_cov_3.432675"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKJ17_08215"
     CDS             <1..663
                     /locus_tag="PKJ17_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD75997.1"
                     /translation="AASISEKGLLQPIILKRKENVYEIIAGERRYRAAILAGLREVPA
                     MVRDVDDREALEIALMENLQREDLGPLEVAAVYERFVEEFTYTHEDIAKKMGVDRSSV
                     TNCLRLLKLPAWVKELMSEGKLTQGHGRVILSLKGEREQKRFVEKVLHEGTSVRELER
                     QARKASPTKEPALAFLEETLVKALQTKVNITMRRNRGKIIIEFYSREDLERLSELITG
                     LN"
     gene            796..1221
                     /locus_tag="PKJ17_08220"
     CDS             796..1221
                     /locus_tag="PKJ17_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOD75998.1"
                     /translation="MINFDATILYQFVHFLILLFILNFILFKPVLRALEKRQNAIKGL
                     TDGVEQAKQDTVDLEKNYESSFVEKRKPIIGGRDAVISEANKEAVHVIEKARSELSEE
                     LAKIKSEIEVEGRKVYDALRADVGKLSMEAAQKILKRSI"
     gene            1221..1715
                     /locus_tag="PKJ17_08225"
     CDS             1221..1715
                     /locus_tag="PKJ17_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOD75999.1"
                     /translation="MAPGGESVWSLIFKFFNFFVLVGLLIYFVGKPLKGFLRKRHQTV
                     KTQLEETQKALSEAQALRARYQEKMDKLEGEMEAFKKQTMQEAETEKKKIIDEAMGFA
                     ARIREQARLTAEQENKEVARRIKEEISRLTVEQAEKLITEKITQSDHDSLVEDFIVKL
                     RSMN"
     gene            1712..2254
                     /gene="atpH"
                     /locus_tag="PKJ17_08230"
     CDS             1712..2254
                     /gene="atpH"
                     /locus_tag="PKJ17_08230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOD76000.1"
                     /translation="MISQSIAKRYAKGLFNVGEKNGKYKEYQSEIEGILGVFDKEERL
                     KRAIVLPLVEVEKRREVLSDVLKSLQVSSPLASMFTMLLEKNRMGYLSLIRDVYNELV
                     DEKEGRVKGTLWTAFPMDDAAKGKIEKELGAKLGKEVLLTVQEDKSLIGGVKVAIKGT
                     IIDGSVKRQLHTLQENILKE"
BASE COUNT          634 a          517 c          701 g          431 t
ORIGIN      
        1 gcggcgtcca tcagtgaaaa ggggcttctc cagcctatca tcctgaagag aaaggaaaac
       61 gtctacgaga tcatcgccgg ggagaggcgc taccgggcgg ccattctggc gggcctgagg
      121 gaggtcccgg ccatggtccg ggatgtcgac gacagggagg cgctggagat cgccctcatg
      181 gagaacctcc agagagagga ccttggtccc ctggaggtgg ccgcggtgta cgagcggttc
      241 gtggaggaat tcacgtacac ccacgaggac atcgcgaaga agatgggcgt tgaccgcagc
      301 tccgttacga actgcctgcg tctcttgaag ctgcccgcat gggtgaagga actcatgtcg
      361 gagggcaagc tcacgcaggg gcacggacgg gtcatccttt cgcttaaggg tgaaagggaa
      421 cagaagaggt ttgtagaaaa ggtccttcat gaggggactt ccgtccggga actggagaga
      481 caggcaagga aggcgtcccc taccaaggag cccgcgctgg catttctgga ggagacgctg
      541 gtcaaggccc tgcagacgaa ggtgaacatc acaatgagaa ggaacagggg caagatcatt
      601 atagaattct attcgcggga ggatctggag cgcctgtctg agttgataac cggcttgaat
      661 taacggctgt atcggggttg aagcacatca atcagcgttt cttgaaaata tgtgacaaaa
      721 ataattgaaa tgccgacggg aattgtgtta aaaccctacg gatagttaaa cgccaatcta
      781 attaggaagt tccctatgat taacttcgat gcgaccattt tgtaccagtt tgttcacttc
      841 cttatcctgc tttttatcct gaacttcatc cttttcaaac cggtcctcag ggccctggaa
      901 aaacgccaga acgccattaa agggttaacg gacggtgtcg aacaggcaaa gcaggacacg
      961 gtagacctgg agaagaacta cgagtcctcc ttcgtggaaa agagaaagcc catcatcggc
     1021 ggcagggatg cggtcatctc ggaggccaac aaggaagccg tgcatgtgat cgagaaggcc
     1081 aggagcgaac tgtccgaaga gcttgcgaag ataaagagtg agatagaagt ggaaggcagg
     1141 aaggtctatg acgcgcttag agccgatgtc ggcaagcttt ccatggaagc ggcgcagaag
     1201 atcttgaaaa ggagtatctg atggcacccg gtggcgagtc ggtctggagt ctaatattca
     1261 aattcttcaa tttctttgtt ctggtcggcc tacttatcta ttttgtcggt aagcccctga
     1321 agggtttcct caggaagcgt caccagacgg tgaagacgca actcgaggaa acacagaagg
     1381 ccttgagcga ggcccaggcc ctgcgtgccc ggtaccagga gaagatggac aagctcgagg
     1441 gcgagatgga ggccttcaag aagcagacga tgcaggaagc ggaaacggaa aagaagaaga
     1501 tcatcgacga ggccatgggc ttcgctgcga gaatacggga acaagcccgc ctgacggcag
     1561 agcaggagaa caaagaggtg gcccggagga tcaaggaaga gatctcccgt ctcaccgtgg
     1621 aacaggcgga gaaactcatc accgaaaaga tcacccagtc cgaccatgac agtctggtgg
     1681 aagatttcat cgtaaaactg aggagcatga attgataagc caatccattg ccaagagata
     1741 tgctaaaggt ctgttcaacg tgggtgaaaa gaacgggaaa tacaaggaat accagtctga
     1801 gatagaaggc atccttggtg tcttcgacaa ggaagagcgc ctgaaaaggg ccatcgtgct
     1861 gcccctcgtg gaggtggaaa agcgccgtga ggtcttgagc gacgttctca agtcgcttca
     1921 ggtctcctct ccgctcgcca gcatgttcac catgctcctt gagaagaaca ggatggggta
     1981 cctatcgttg atcagggatg tctacaacga actcgtcgac gaaaaggaag gcagggtcaa
     2041 gggcaccctt tggacggcgt tccccatgga tgatgccgcg aaggggaaga tagagaagga
     2101 actcggggcg aaactcggga aagaggtcct gctgacggta caggaagaca aaagccttat
     2161 cggcggggtc aaggttgcaa ttaagggaac gataatagac ggaagcgtga aaagacagct
     2221 gcacacgttg caggaaaata tactgaagga gtagtgtcta tggagatcag ggccgacgaa
     2281 ata
//
LOCUS       DAOPOA010000389         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_51259_length_2282_cov_2.693758, whole genome shotgun sequence.
ACCESSION   DAOPOA010000389 DAOPOA010000000
VERSION     DAOPOA010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51259_length_2282_cov_2.693758"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKJ17_08235"
     CDS             <1..260
                     /locus_tag="PKJ17_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOD76001.1"
                     /translation="PDFEKSLDELITAGDRVILIDLSALGYISSAGLRSILSSAKKLK
                     VLSGEILFTGLQGPVEEVFQISGFKSIFKIFPSEAEALGSM"
     gene            263..688
                     /locus_tag="PKJ17_08240"
     CDS             263..688
                     /locus_tag="PKJ17_08240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD76002.1"
                     /translation="MAENIHTLVSIELPAVLESLPAFSARMMECAKEAGFKEHKLGAM
                     ELALEEAIVNIVKYSSSSPDSVITAKCGTDNSGAFYITIIDSGPPFDPLKKGAPDTGA
                     GIDDRPVGGLGIFFIKEMTDGVTYTREAGKNILTIKMKR"
     gene            complement(867..1820)
                     /locus_tag="PKJ17_08245"
     CDS             complement(867..1820)
                     /locus_tag="PKJ17_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD76003.1"
                     /translation="MPASITAETVRAKIVRAIRAAESRTFENDSDIENFIRDELPFTV
                     GRTYGSNTPCVEIKNGEEYIICDAGTGLRDLGNHHMKLIEQGLQRRSGSVFNIFVSHF
                     HWDHIQGFPFFTPAYIPGNKVRIYGFHKNLKGFFEKQQNQPYFPVPLASMSADIQFIV
                     LKPGREYEISGFEISGIEQKHPGTSYGFRFQKDGRTVVYSTDAEHKTSYDSTAREENY
                     PFLDFFREADILIFDAQYDWNEAVLSKDDWGHSSYVAAVELAVKAKVKHLCLFHNEPT
                     YSDERLDVLLEDTRNYLKIYNDHQPHPMKVDLAYDGYEIEV"
     gene            complement(2052..>2282)
                     /locus_tag="PKJ17_08250"
     CDS             complement(2052..>2282)
                     /locus_tag="PKJ17_08250"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOD76004.1"
                     /translation="GLFLYTDGVTEAMDEAGEIFSTDRLKDVLATCADGDVSGITACV
                     GGAINDFCRDAPQYDDITMMAVRYEGTGNENG"
BASE COUNT          518 a          634 c          594 g          536 t
ORIGIN      
        1 cgccggattt cgagaagagc ctcgatgagc tcatcacggc gggagacagg gtcatcctca
       61 ttgatctgtc cgccctcggc tacatcagca gcgcggggtt gaggagcatc ctctcgtcag
      121 ccaagaagct caaggttttg tcgggggaga tactctttac cgggcttcag ggacctgtcg
      181 aagaggtctt ccagatatcg ggtttcaaat ccattttcaa gatctttccc tcggaagcgg
      241 aagccctcgg aagcatgtga gaatggcgga gaacatacat accctcgtga gcatcgagct
      301 cccggcagtg cttgagtccc tgccggcatt ctcggcacgt atgatggagt gcgcgaagga
      361 ggcaggcttc aaggagcaca agctcggcgc catggagctg gcactggaag aggccattgt
      421 aaacatcgtc aagtattcct cctcatcccc cgacagcgtc atcaccgcaa aatgcggcac
      481 agataacagc ggcgcgttct acatcaccat catcgacagc ggcccccctt tcgatcccct
      541 gaagaagggc gcgcccgaca ccggggccgg catcgacgac cggcctgtcg gcggtctcgg
      601 catattcttc ataaaagaaa tgaccgacgg cgtgacctac acccgcgaag cgggaaagaa
      661 cattctcacc ataaaaatga aaagatgata atgaccaatc accaaatctc aatgaccaat
      721 taagaaaaca atgaccaatt tcccaaacac aaatgggggc agaattctcc cgtggtcttc
      781 cgtttggtta ttgtggtttg gtgaattggt tattccctgg ttattgggac ctggttattg
      841 tcttcttcaa cgcttgttat ctgttctcag acttctattt cgtacccgtc atacgccagg
      901 tcgaccttca tggggtgggg ttggtgatcg ttgtagatct tgagatagtt cctcgtgtcc
      961 tcgagaagga cgtcgagacg ttcgtcactg tatgtgggtt cgttgtggaa aaggcacaga
     1021 tgcttcacct ttgccttgac ggcgagctcc accgccgcta cgtaactgga atgaccccag
     1081 tcgtccttcg aaaggaccgc ttcgttccaa tcatactggg catcgaagat gaggatgtcg
     1141 gcttccctga aaaaatccag gaaagggtag ttctcctccc tggcggtgct gtcatagctt
     1201 gtcttgtgtt cggcgtcggt ggaatagacc acggtccgcc cgtccttctg gaaccggaaa
     1261 ccgtacgagg tccccgggtg cttctgctcg atgcccgaga tctcgaaacc gcttatctcg
     1321 tactccctgc cgggtttgag gacgatgaac tgtatgtcgg cgctcatgct cgcaagggga
     1381 acggggaaat agggctggtt ctgctgcttc tcgaagaagc ccttcaggtt cttgtggaaa
     1441 ccgtatatcc tcaccttgtt tccggggata taggccggcg tgaagaaggg gaacccctgg
     1501 atatggtccc agtgaaaatg ggagacgaag atgttgaaga cggaaccgct cctgcgctgc
     1561 agaccctgtt cgatgagctt catatgatgg ttgccaaggt ccctgagacc cgtgccggca
     1621 tcgcagatga tgtactcttc gccattcttg atctccacgc agggtgtgtt gcttccgtac
     1681 gtccttccta ccgtgaaggg aagctcgtcg cgaatgaagt tttctatgtc gctgtcgttc
     1741 tcaaacgtcc gcgactcggc cgccctgata gctcttacga tcttggcccg aaccgtctcc
     1801 gcagttatag acgctggcaa agaccctcgt gtgccccaga aatagacgcg catccctgct
     1861 cccggtgaat tagatgcatt attctaacct cttgcggata aattgcaaga ttattttaaa
     1921 cattgtagtc acaaagagct aaaccaacaa tgacaaacat cacaaagaga caaaacgccg
     1981 tgggtgatag gtgatgggtc acaggttatg ggtggaaacc gcctccccca tcatccatta
     2041 cctatcaccc atcacccgtt ctcattgccc gtcccctcat atctcaccgc catcatggtg
     2101 atgtcgtcgt actgcggtgc atccctgcag aaatcgttga tggcccctcc gacacatgcg
     2161 gtgatcccgg agacatcccc atcggcacac gtggcaagca catctttgag gcgatccgta
     2221 gagaatatct cgcccgcctc gtccatggct tccgtgacgc catccgtgta cagaaacaac
     2281 cc
//
LOCUS       DAOPOA010000390         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_51278_length_2281_cov_4.214286, whole genome shotgun sequence.
ACCESSION   DAOPOA010000390 DAOPOA010000000
VERSION     DAOPOA010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51278_length_2281_cov_4.214286"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PKJ17_08255"
     CDS             <1..921
                     /locus_tag="PKJ17_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76005.1"
                     /translation="EEVTPLGPFSKLPLIVTEKEEELERAVRYGFELSEEYRIPVIVQ
                     AVPAGYDKGRETAGQTEKTAGNAAMLVKEPGRWAATPKFRFQLHGELNEKIRKIGEVF
                     ETYEDNLIISKGSTGLVTDRLSRLEFYDEDASVLRLATVFPLPQRLVQGFVDGMDEVL
                     VVETYPAIELQIPDRSKVKRGFVDTGRGRPQYDEIIAGFSVIRDRLGPASSINMAHGI
                     KRLHPERNILAVTFEDHFFHSGMPAYVNSLYNGSSFVLLVMVSEREDDLRRILRGYGV
                     SGVHSISNIDEIGRFRDTKEPVVALYRGMI"
     gene            918..1505
                     /locus_tag="PKJ17_08260"
     CDS             918..1505
                     /locus_tag="PKJ17_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOD76006.1"
                     /translation="MRHEIICAGIGGRGVLLASTILIEAAIGSGLKAVASDEYGMSQR
                     GGSVVSLVKVGDFASPFIGRENGGILLAFEESEFYKALLFLKKGGLALVNTRAAELPP
                     SIAGLLEKREITCRLIDADGIARQTGMFQSANMALLGFFAAFGIAPFTKEVLEETIRS
                     RVPEKFLAKNLEVFGKGFDAAMKMGNAGDRRTDMV"
     gene            1527..>2281
                     /locus_tag="PKJ17_08265"
     CDS             1527..>2281
                     /locus_tag="PKJ17_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76007.1"
                     /translation="MHSREEVVIGNQAIARGLVEAGLEIAAAYPGTPSSEILPGVVYF
                     RDMEGSKIHTEWSTNELCAFEVAFGAAAYGGARAACFMKQVGLNVAFPALMRSREKSI
                     EGGLVIVSCDDPGPQSSQTEQDTRLIATLFGIPVFDPSSPAEAADVAYHALNWSFENK
                     VPVIIRSTHRVSHARESVPLYGPGTRRVAFREGHVAGRGRKLGIVASGMSCSLVYDVM
                     EELGASEEVSLYKVLKVYPVDEALTGFVDSMERV"
BASE COUNT          459 a          645 c          747 g          430 t
ORIGIN      
        1 gaggaggtga cccctctcgg cccgttctcg aagctgcccc ttatcgtcac cgaaaaggaa
       61 gaagaactgg aaagggccgt caggtacggg ttcgaacttt ccgaggagta caggatcccc
      121 gttatcgtgc aggccgtccc ggccgggtac gacaaaggcc gggagactgc ggggcagact
      181 gaaaagacgg cagggaacgc ggccatgctc gtaaaggagc cgggcaggtg ggcggcgacg
      241 ccgaagttcc gtttccagct tcacggggag ctcaacgaaa agatcaggaa gatcggcgag
      301 gtcttcgaga cctatgagga caacctcatc atctcaaagg gttcaacggg actcgtcacg
      361 gaccggctgt cccgtctcga gttctacgac gaggacgcga gcgtgctcag gctggcaact
      421 gtctttcccc tgccgcagag gctcgtgcag ggattcgttg acggcatgga cgaggtcctc
      481 gtcgtcgaga cctatccggc gatcgaactc cagataccgg acagatcgaa ggtgaagcgg
      541 gggttcgtcg ataccggacg cggcagaccg caatacgatg agatcatagc agggttctcc
      601 gtaatcaggg accggctggg ccccgcctct tcgataaaca tggcgcacgg catcaagagg
      661 ctccatcccg agaggaacat cctggccgtg accttcgagg accacttctt ccattcaggg
      721 atgccggcgt acgtcaacag cctctacaat ggctcttcgt tcgtacttct cgtcatggtg
      781 agcgagaggg aggacgatct gagaaggata ctgcggggct atggcgtaag cggggtgcac
      841 tcgatctcga acatcgacga gatcggccgg ttccgggaca cgaaggagcc cgtggtggct
      901 ctctacaggg gtatgatatg agacatgaga tcatatgcgc cggtatcggg ggcaggggtg
      961 tgctcctcgc ctccacgata ctcatcgagg ccgccatcgg gagcgggctc aaggcggtgg
     1021 catccgatga atacggtatg agccagaggg gagggtccgt cgtgtccctc gtgaaggtgg
     1081 gcgatttcgc gagtcccttc atcggcaggg agaacggcgg catcctcctc gccttcgagg
     1141 agagcgagtt ctacaaagcg ctcctcttcc tgaagaaggg cggccttgcc cttgtcaaca
     1201 cccgtgcggc tgagctgccg ccgtccatcg cgggtcttct ggagaagcgg gagataacct
     1261 gccgcctcat cgatgccgac ggtattgccc gtcagacggg catgttccag tccgcgaaca
     1321 tggcccttct cggctttttc gccgccttcg gcatcgcccc ttttacgaaa gaggttctcg
     1381 aagagacgat caggtcccgc gtgccggaga agtttttggc aaagaatctc gaagtcttcg
     1441 ggaagggatt cgacgccgcg atgaaaatgg gcaacgcggg ggaccgccgg acggatatgg
     1501 tttaagagct tatgggagga tcgtcagtgc atagcaggga agaagtggta ataggcaatc
     1561 aggccatcgc gaggggcctt gtcgaggcag gccttgagat agcggcggca tacccgggga
     1621 cgccgagttc cgagatactg cccggagtcg tctatttcag ggacatggag ggatcgaaga
     1681 tccacaccga gtggtcgacg aacgagctct gcgccttcga ggtcgccttc ggggccgcgg
     1741 cctacggcgg ggccagagcg gcctgtttca tgaagcaggt gggcctcaat gtagcgttcc
     1801 cggcactcat gaggtcccgg gaaaagtcca ttgagggagg gctcgtgatc gtctcctgtg
     1861 acgaccccgg cccgcagtcg tcccagacgg agcaggacac gcggcttata gcgaccctct
     1921 tcgggatccc cgttttcgat ccgtcatcgc cggctgaggc ggccgatgtc gcctaccacg
     1981 ccctcaattg gtccttcgag aacaaggtac ccgtcatcat ccgttccacc caccgggtta
     2041 gccatgcccg cgagtcggtt cccctctacg gcccggggac gaggagggtg gccttcaggg
     2101 aaggacacgt tgccggaagg ggccggaagc tgggcatcgt cgcgtcgggc atgagttgct
     2161 ccctcgtcta cgatgtcatg gaagagctgg gcgcgtcgga agaggtttcc ctttacaagg
     2221 tcctcaaggt ctatcccgtc gacgaggccc tcacggggtt tgtcgattcc atggaacgcg
     2281 t
//
LOCUS       DAOPOA010000391         2275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_51468_length_2275_cov_3.539640, whole genome shotgun sequence.
ACCESSION   DAOPOA010000391 DAOPOA010000000
VERSION     DAOPOA010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51468_length_2275_cov_3.539640"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1175)
                     /locus_tag="PKJ17_08270"
     CDS             complement(159..1175)
                     /locus_tag="PKJ17_08270"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOD76008.1"
                     /translation="MKRCVSIVSVVLFLAVTFCLTSVPASAQVKLRFATFFPVSHPNA
                     AITAEWCKEVEKRTNGKVKVQHFPGATLTSPQSQYDSILTGVVDIANCVLGYTMGKFP
                     LSEILDYPLGYPSGAVATRLGNEFYKQFKPKEFDDVKVMYFHAQGPGILHTRKPVKNL
                     DDLKGMKIRTFGSNAKMMSMLGGSPVAMPMGDAYDALSKGVADGLLCGYEALKGWKLG
                     EVIKSTTENYGTAYTATFVIAMNKAKWNSIPPDAQKVIEEINKEFIEKQGKLWDKMDQ
                     DGKEFSVKRGNQVIKLSAEENQKWITKIEPLFAEYVKKMKEKNLPGDKALKFVREYIK
                     KNTK"
     gene            complement(1497..>2275)
                     /locus_tag="PKJ17_08275"
     CDS             complement(1497..>2275)
                     /locus_tag="PKJ17_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD76009.1"
                     /translation="SQFVAVNSSTLQESILESELFGYEKGAFTGAQADKMGLLELADK
                     GTFFIDEIGDMGMAIQAKFLRTLETGIFRRLGDTRESKVDVRFIFATNKSLEAEVKEK
                     RFRKDLFFRLNTFIITLPPLRERKDDMPLLADYFLGKFARGAVKKALSREAMELLKDY
                     RWPGNVRELANVMERAVLLCASRETILPEDLPQSMMEGRFADREADRRELTSADELSL
                     HHIEQEHIERVLTLAKGNKSKAARLLGISRRKLYLKTQEK"
BASE COUNT          411 a          674 c          645 g          545 t
ORIGIN      
        1 gtgagcatca tcatcaggaa cagaacgatg ccgctcaacc cgttcatcca gccgctgaac
       61 ctcctgacgg ctccgaccac gctgttcatc gatcatcctc cttagggagc cggctgccgg
      121 gcccgttcac tcttcccggc agcctggcct tatgggggct atttcgtgtt cttcttgatg
      181 tactcgcgaa cgaacttgag cgccttgtcg cccggaaggt tcttctcttt catcttcttg
      241 acgtattcgg cgaagagagg ctcgatcttc gtgatccatt tctggttctc ttcggcggaa
      301 agcttgatca cctggtttcc ccttttcacg gagaattcct tcccgtcctg gtccatcttg
      361 tcccagagct tgccctgttt ctcgatgaac tccttgttga tctcctcgat caccttctgg
      421 gcgtcgggag gaatgctgtt ccatttcgcc ttgttcatgg cgatgacgaa ggtggcggtg
      481 taggcggtgc cgtagttctc cgtcgtggac ttgatgacct cgccgagctt ccatcccttc
      541 agggcctcgt aaccgcacag gaggccgtcg gcaacgccct tggagagggc gtcataggca
      601 tcgcccatcg gcatggcaac cggcgagccg ccgagcatgg acatcatctt cgcgttggag
      661 ccgaaggtcc tgatcttcat gcccttcaga tcatcgaggt tcttcacggg ttttctcgtg
      721 tggagtattc ccgggccctg ggcgtggaag tacatgacct tcacgtcgtc gaattctttg
      781 ggcttgaact gtttatagaa ctcgttgcca agcctcgtcg cgacggcgcc gctcggataa
      841 cccagcgggt agtcgaggat ctcggagagg gggaatttcc ccatcgtgta cccgaggaca
      901 cagttcgcga tgtccacgac accggtcagg atgctgtcat actgggactg gggcgacgtc
      961 agggtggcac ccgggaaatg ctggaccttc acctttccgt tcgtcctctt ctcgacctcc
     1021 ttgcaccact ccgccgtgat tgccgcgttg ggatggctca cagggaagaa ggtggcgaaa
     1081 cgaagtttca cctgggcgct cgccggcacc gaagtgaggc agaaggtcac tgcaaggaac
     1141 aggacaacgg atacaatact cacacaccgt ttcataagaa acccccttga ttttggattt
     1201 gctgacattt ttgaaaagca atttcgatgc caccccttaa acccccgtaa gaccccgaaa
     1261 tcagtgactc ccgctcaact ccgttcagcc gggtgtgcat aattgataca tgggtgtgca
     1321 tcttttaacc acatggctgc ggcccggggg ccgcagccgg cttgaaccgc ttgaaccgct
     1381 tgaacgttag aaacaggaac cttaccgcta cctcgggacg tgactgaaaa gtcaggaccc
     1441 ttcagcgcca gctccgtctt tcaacgttca agcgactcaa gcggttcaag cggtctttat
     1501 ttctcctgtg tcttcaagta cagttttctc ctgcttatcc ccaggaggcg ggcggctttg
     1561 ctcttgttgc ccttggcgag ggtcaggacg cgttcgatgt gttcctgttc gatgtggtgg
     1621 aggctcagct catcggcgga ggtgagctcc cgccggtccg cttcgcggtc tgcgaaacgg
     1681 ccctccatca tgctctgggg gaggtcttcg ggaaggatcg tctcccggga ggcacagaga
     1741 agcacggccc gctccatgac gttcgccagt tcgcggacgt tgccgggcca gcggtagtcc
     1801 ttgaggagct ccatggcctc gcgggagaga gccttcttca ccgcgccgcg ggcgaacttg
     1861 ccgaggaagt agtcggcgag gagcggcatg tcatccttgc gctcccttaa gggcggcagg
     1921 gtgatgatga aggtgttgag ccgaaagaag aggtccttgc ggaacctttt ttctttcacc
     1981 tccgcctcta gggacttgtt ggtggcgaat atgaacctca cgtccacctt tgactccctc
     2041 gtgtccccga ggcgccggaa gatgcccgtc tcgagggtcc tcaggaactt ggcctggatg
     2101 gccatgccca tgtcgcctat ctcgtcgatg aagaaagtgc ccttgtcggc gagctcgaga
     2161 agacccatct tgtccgcctg tgcccccgtg aaggcgccct tctcgtagcc gaagagctcg
     2221 ctttccagga tgctctcctg gagcgtgctt gaatttacgg ccacgaactg actcg
//
LOCUS       DAOPOA010000392         2273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_51529_length_2273_cov_2.996393, whole genome shotgun sequence.
ACCESSION   DAOPOA010000392 DAOPOA010000000
VERSION     DAOPOA010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51529_length_2273_cov_2.996393"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..1828)
                     /locus_tag="PKJ17_08280"
     CDS             complement(317..1828)
                     /locus_tag="PKJ17_08280"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOD76010.1"
                     /translation="MTKKILGTKFAIQKRGGATGPAADTGAAEKMKKTDKLEQKIREL
                     EERNNDLLAMIENSYDAMAIADGESRLLLLNPAFERVMGIKNADIIGRKIEELVREGI
                     SDTGATLKVLETGKAQTVLINTIAGRQVLSTGIPVFDKHGRISKVYCNLRDITELNHL
                     KEKYKQSQKLVSKYLLELHEARRMQKAPSAFVAHSERMKQVIETAYRIARIDTTVLIL
                     GESGVGKDLVARLIHEASPRMETGTFVKINCGAIPPDLLESELFGHEAGAFTGARREG
                     KAGYFEIADKGTIFLDEIGDLPLRLQVKLLSVIQDQEVARVGGTKPKKVDARIIAATN
                     RDVEKMVKTGNFREDLYYRLNVVPFHIPALRERREEIPFLIKHYLNEFNSRYNLKVKL
                     TQEVVERLIAYRWPGNVRELANLVEQLVVLANEDLVTVEHLPKRFSAPERGDLAEMRE
                     LRPLDESVREFEIKLIRTALDQSKSNEEAAHKLGISLSTLARRLRQFKPDSHS"
     gene            2012..>2273
                     /locus_tag="PKJ17_08285"
     CDS             2012..>2273
                     /locus_tag="PKJ17_08285"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOD76011.1"
                     /translation="MEGMTVSVPRRMSTLVLGAVTFCLVTLLLTGCAGIPDGVEAVKG
                     FDVNRYLGTWYEIARLDHPFERGLSKVRAEYTLREDGGVNVIN"
BASE COUNT          418 a          631 c          672 g          552 t
ORIGIN      
        1 cgtcagggaa aagattccgc cgatcttgat gggttctttc gaccatgcgg gaccggcagc
       61 gaccagggca acaaagacca tgagaacaaa aacggaaaaa acgaatcttt tcattccgta
      121 cccccttgaa gtgttggttt acgaaatgac aaattgcaag tcgggtgcca gcactgaaaa
      181 cctcgttatt tcggtctcat agggatttgg agatgtcccg caaacgcgcg gacccctggg
      241 gacatgtcat ttttgacatg gcaatccagg ggacagggtc cgtttcgctc aatgatcttg
      301 ggcttgtgag gacgtgtcag gaatgactgt caggtttgaa ctgtctgaga cgtcttgcca
      361 gagtggagag gctgatcccg agcttatggg ccgcctcttc gttggacttt gactgatcga
      421 gggccgtcct gatgagcttg atctcaaatt ccctcaccga ctcgtcaagg ggcctcaatt
      481 cccgcatttc agcaaggtcg ccgcgctccg gggcgctgaa ccttttcgga aggtgctcga
      541 ccgtcacaag atcttcgttg gcgagcacga cgagctgctc cacgaggttg gcgagctcgc
      601 ggacgttgcc gggccacctg taagcgatca accgttcgac gacctcctgg gtcagtttca
      661 ccttcaggtt gtatctgctg ttgaactcgt tgagatagtg tttgatcagg aagggtatct
      721 cttcccgccg ctctctgagg gccggtatgt ggaagggaac gacgttgagc cggtaataga
      781 gatcttccct gaagttgccg gtcttcacca tcttttccac gtccctgttc gtagccgcta
      841 ttatcctcgc gtccaccttc ttgggttttg tgcctcccac gcgtgccact tcctggtcct
      901 ggatgacgga aaggagcttc acctgcaggc gcaggggaag gtctccgatc tcgtcgagga
      961 agatcgtacc tttatcggct atctcgaagt atcccgcttt cccctctctc ctggcacccg
     1021 tgaaagcgcc ggcttcgtgg ccgaagagct ccgactccag gagatcgggg ggtatggcgc
     1081 cgcagttgat cttcacgaag gtgcccgttt ccatccgggg agaggcctca tggatgaggc
     1141 gtgcgacgag gtctttgccc acgcccgact caccgaggat cagcaccgtc gtgtcgatcc
     1201 gggctatccg gtaggcggtc tcgataacct gcttcatccg ttcgctgtgg gccacgaagg
     1261 ccgaaggggc cttctgcatt cgtctcgcct cgtggagctc aaggaggtat ttcgacacga
     1321 gtttctgcga ctgcttgtat ttttccttga ggtggttcag ctcggtgatg tcccgcaggt
     1381 tgcagtacac cttggagatc ctgccgtgct tgtcgaagac gggtatgccc gtggagagca
     1441 cctgtctccc ggcgatggta ttgatgagca ccgtctgggc tttgcccgtc tccaggactt
     1501 tgagcgttgc cccggtgtcc gagatgcctt ccctgacgag ctcttcgatc ttgcggccga
     1561 tgatgtcggc gttcttgatg cccatgaccc tctcgaatgc cggattgagg aggaggagcc
     1621 ggctctcgcc gtcggcgatg gccatggcgt cgtaggagtt ctcgatcatc gcgaggaggt
     1681 cgttgttgcg ttcctcgagc tcgcggatct tctgttcaag cttgtcggtc tttttcatct
     1741 tttccgcagc cccggtgtcg gcggcaggac ccgtggcgcc gccgcgtttt tggattgcaa
     1801 atttcgtacc caggatcttc ttcgtcatcg cactccacac cccggcttgc cgcgggtatc
     1861 ctgctgatct ctcgatggat gacgcccggc atcattcccg tacgctgaga ttctaccatt
     1921 gagagaagaa aaatcaacac cctcctcgcg atcgcaaggc ctggcagaga ccgggagccc
     1981 ctatttacat tgcagttggc gggggtgtaa tatggaagga atgacagttt ccgtgccgcg
     2041 aaggatgtcg acactcgttc ttggggcagt caccttttgc ctggtgaccc tgctgctcac
     2101 cggatgcgcg gggattcctg acggggtcga ggccgtaaag gggttcgacg tgaacaggta
     2161 cctggggacc tggtacgaga tagcgagact cgaccatcct ttcgaacggg gcctttcgaa
     2221 ggtccgggcg gaatataccc tgagggaaga cggcggtgtc aacgttatca aca
//
LOCUS       DAOPOA010000393         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_51591_length_2271_cov_3.771209, whole genome shotgun sequence.
ACCESSION   DAOPOA010000393 DAOPOA010000000
VERSION     DAOPOA010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51591_length_2271_cov_3.771209"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..723)
                     /locus_tag="PKJ17_08290"
     CDS             complement(121..723)
                     /locus_tag="PKJ17_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76012.1"
                     /translation="MGHHSLIAWIREEIMSTEVVMRSEVVGYTERLEPNPAMSISEGT
                     LYPTTSDGMYEIHQDPIYEDVPRTVLDERRASDAAEALVTLILSDERYMYLASELMGG
                     EKVFVPHWDRLCRMGRDRRTRRLALHRLGKAPSQLFMEGVRGMAEALKGPLTSLWYLC
                     AGACFYAFIIYLIWPDSKAGRWIAATVKRILAEATRMIYD"
     gene            complement(745..1032)
                     /locus_tag="PKJ17_08295"
     CDS             complement(745..1032)
                     /locus_tag="PKJ17_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76013.1"
                     /translation="MTRDEEIRYGMEAYRDALRFAAGASTREDLAGVEESYWIAFNKL
                     RESPLEEHRRICAECLEAVARILDGEGRRDAGNDLREQAETFRKPRAGEKR"
     gene            complement(1145..1339)
                     /locus_tag="PKJ17_08300"
     CDS             complement(1145..1339)
                     /locus_tag="PKJ17_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76014.1"
                     /translation="MGIIDTGTDPGEHNIISNASRAAILPAHIVKAPLLPSLPIIGHR
                     PLPIITGRMRGFDLSCHPLF"
     gene            complement(1339..>2271)
                     /locus_tag="PKJ17_08305"
     CDS             complement(1339..>2271)
                     /locus_tag="PKJ17_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOD76015.1"
                     /translation="EGPLALYVAGDGQRLVAVSGHSGFLGSYNLGTGTPIAGFPLVGM
                     EPFQAFCMGGQVLATASRTVLSFRDERTGRLLKRCDHNDYIDDILFVPSRERVIAAGL
                     RGFLFWDREGSEPVMEKPDNADGVITFGGCPSLSPGETNIIVRGRYYIALWDIETHRF
                     LWRHDFPRENRIRKVVAFPDGERFATLDITGVITVRSVDKGDVLATLHVLPEGFIWET
                     PPDDHAPSGWLWTDREDLITVTAASGNDGGSRIFREGESEHRAYLRIYNNREMVTAKI
                     AGKEEYRQQVALHARVLDRARIDNGSVGLRPALP"
BASE COUNT          428 a          684 c          672 g          487 t
ORIGIN      
        1 ggggttcgta tgaaaggacc gtatgaccgc gtccatgtcg tcgccgagtt tgctgtaggg
       61 tgtcattttc catctccttt ccttttcagc ggaagagggt cgcaaatgag cggtttcctt
      121 ctaatcgtat atcattcttg tcgcttccgc gagaattctc tttaccgtcg cggcgatcca
      181 cctgcccgcc ttcgagtccg gccagatgag gtagatgata aacgcgtaaa agcatgcacc
      241 ggcgcagaga taccagagag acgtgagcgg acctttcagc gcttccgcca tcccgcgtac
      301 accctccatg aaaagctgag aaggggcttt gccaaggcga tgaagggcca accttcgtgt
      361 ccttctgtcc ctacccatcc ggcagaggcg gtcccagtgc gggacgaaca ccttttctcc
      421 gcccatcagc tcgctcgcca ggtacatata ccgctcgtcg gagagtatga gggtgacgag
      481 cgcctcggcg gcatccgacg cgcgcctctc gtcgaggacg gtccggggga catcctcgta
      541 gatagggtcc tggtggatct cgtacatccc gtccgaggtt gtaggataaa gagtgccctc
      601 ggagatagac atggcgggat tgggctccaa tctctctgta tagccaacaa cctcactccg
      661 catgaccacc tccgtagaca tgatctcttc ccgtatccag gcgatgagac tgtgatgccc
      721 catgttcccc gaccccttct tgcgttatcg tttctctccg gcgcggggct tgcggaaggt
      781 ctcggcctgc tcccgaagat cgttgccggc gtcccgtcga ccctccccat cgagaattcg
      841 cgccaccgct tcaagacact cggcgcagat acgccggtgc tcctcgaggg gagactctct
      901 cagtttgtta aaggcgatcc agtagctctc ctccacaccg gcaaggtctt cccgcgtgga
      961 agccccggcc gcgaaccgca gggcatccct gtatgcctcc atcccgtacc ttatttcctc
     1021 gtctcttgtc atggtcacct ccattgacgg atcatagagc gttttttaat tggatatcag
     1081 gcaaagtccc gtgcacgcaa tggcaaaaga gaaggtgtat catgcacaaa gtggggattg
     1141 ggggctaaaa aagcggatgg caggagagat cgaagcctct catccttccc gtgatgatcg
     1201 gcaggggccg gtgcccgata atgggaaggg acgggaggag gggagccttg acgatgtggg
     1261 cagggagaat ggcggcgcgg gaggcgttgg agatgatgtt gtgttccccg gggtcggttc
     1321 cggtgtcgat gatgcccatc agggcagggc ggggcgaaga ccgacggagc cgttgtcgat
     1381 ccgtgccctg tcgaggacgc gggcgtggag cgcgacctgc tggcggtact cttcctttcc
     1441 cgcgatcttc gccgtgacca tttcccggtt gttgtatatc ctcaggtagg ccctgtgctc
     1501 gctttcaccc tctcggaata tccttgagcc gccatcattc cccgacgcgg ccgtgacggt
     1561 gataaggtct tccctgtccg tccagagcca ccccgaaggt gcatggtcat cgggcggggt
     1621 ctcccagatg aacccttcgg gcaggacatg gagggtcgcg aggacatccc ctttatcgac
     1681 gctacggacc gttatgaccc ccgttatgtc cagtgtcgcg aagcgttcgc cgtcgggaaa
     1741 ggcgacgacc tttcttatcc ggttctcccg gggaaagtcg tgccgccaca ggaaccggtg
     1801 tgtctcaatg tcccacaggg ctatgtagta ccggccccga acgatgatat tcgtttcgcc
     1861 gggcgataag gacgggcatc cgccgaatgt gatgacgccg tccgcattgt ccggtttctc
     1921 catcacaggt tcgctccctt cccggtccca gaagaggaac cctctcaatc ccgcagcgat
     1981 caccctttcc cgggacggga cgaagaggat gtcgtcgatg tagtcgttgt ggtcgcatct
     2041 cttcaggagc ctgcccgttc gttcatcacg gaatgaaagg accgttcggc tcgcggtggc
     2101 gaggacctgt ccacccatgc agaatgcctg gaacggctcc atgccgacga gggggaaacc
     2161 ggcaataggg gtgcccgttc caaggttgta ggacccgaga aagcccgagt gtcctgagac
     2221 cgccaccagc ctttgcccgt ccccggccac gtagagggct aaaggaccct c
//
LOCUS       DAOPOA010000394         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_51687_length_2268_cov_3.048803, whole genome shotgun sequence.
ACCESSION   DAOPOA010000394 DAOPOA010000000
VERSION     DAOPOA010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51687_length_2268_cov_3.048803"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /locus_tag="PKJ17_08310"
     CDS             <1..1867
                     /locus_tag="PKJ17_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD76016.1"
                     /translation="PVLRSMILVVDDRNTPIVTLRSRRTDLPVTLPLPASGATTRGFT
                     TIDTRDGVRPALFLSIPLEEPKVVLSELVILDNANPLVRNLSDLLSRKRGEPVYVSLF
                     KDDRRMFSTIFSLTGTATQDLPEEITTALQGGRENYIGRTTIGRSGYYTVYKPSPYNE
                     AGSPVWSYGIAVSEDIFMPFKKRLLFVFVMISVLATSAVIIITFAITRGIDPSLRGIL
                     DICAKIEDGNTLSRIDSKRVTTREFTQIASSINRMLDSISEREKTISDNMDRIRAINA
                     ELEEKTNIIRQDRMRFLSILETVDEGVVALDHEGVIIYYNRAAAAITGTDPEAALGDT
                     WKSLFPSLDISGEDHAALQELTIPREDSSEPLYLRVLASPWSVENAPQGRILVLQDIS
                     REKRMEEFKADFVSSINHDIRSFLVPVSGFIGRILEGKYGPLEDAVRERLLNVADNVS
                     KVHHLVENYLNVSRIESGKLDLAIGPVDMNEVIRDVAKLYAPRVIFADGATPQALVLA
                     DKDYIERVITNLVVNALKFSPEDSTVRIGTRVEGGMLVTFVEDRGAGIPARELRFIFE
                     KYRRGSLGRREEGSGLGLFIVKSVVEGHGGSIWVDSVPGRGSTFFFSLPLSRGEI"
     gene            1931..>2268
                     /locus_tag="PKJ17_08315"
     CDS             1931..>2268
                     /locus_tag="PKJ17_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76017.1"
                     /translation="MRKIVIVLLAVVLASCSYMPWGKKKDEDPSKTTKKTDSAAQKKP
                     ARGEPEEMKEPELGDIKVIDGVEYIYTRNRKYMLTPYEPPYVWVRKDQYAPGIGEALS
                     FGIGGSGPSK"
BASE COUNT          507 a          687 c          644 g          430 t
ORIGIN      
        1 gccggtgttg cggtcgatga tcctggtggt tgacgacagg aacacgccca tcgtcaccct
       61 gcgaagccgg cgtacggatc ttcccgttac gctgccgctg cccgcctccg gtgcgaccac
      121 caggggtttc accaccatcg acaccaggga cggagtccgt ccggccctct ttctttccat
      181 acccctcgag gagccgaagg tcgtcctgag cgagcttgtc atcctcgaca acgcgaatcc
      241 tctcgtcagg aacctgagcg atctgctctc ccgcaagcgc ggggagcccg tgtatgtctc
      301 acttttcaag gacgacagga ggatgttcag caccatattc tcgctgaccg gcaccgctac
      361 ccaggacctt cccgaagaga tcacgaccgc tcttcagggc ggccgggaga actacatagg
      421 cagaacgacc ataggaagat ccggctacta caccgtctac aagccgtcgc cgtacaacga
      481 ggcggggtcg ccggtgtggt cctacggaat cgccgtgagc gaggatatct tcatgccctt
      541 caagaaacgc ctcctcttcg tctttgtcat gatatccgtt ctcgcgacgt cggcggtcat
      601 catcataacc ttcgcgatca cgcggggcat cgacccctcc cttcggggca tcctggatat
      661 atgcgcgaag atagaggacg gaaacaccct ttcccgcatc gacagcaaga gggtgacgac
      721 ccgcgagttc acccagatcg cttcgtccat caacaggatg ctcgattcca tctccgaacg
      781 ggagaagacc ataagcgaca acatggacag gatacgggcg atcaacgccg agcttgagga
      841 aaaaacgaac ataatccggc aggaccggat gcggttcctg tccatcctgg agaccgtgga
      901 cgaaggggtt gtcgcccttg accatgaagg cgtcatcatc tattacaaca gggccgcggc
      961 ggccatcacg ggaaccgacc cggaggccgc cctgggcgac acctggaaaa gcctctttcc
     1021 gtcactcgac atctccggcg aggaccacgc ggccctgcag gaactcacca tcccgcggga
     1081 ggactcctcc gaacccctgt atctcagggt ccttgcttcg ccctggtccg tggagaacgc
     1141 tccccagggc cggatcctgg tgcttcagga tatatcgagg gagaagagga tggaggagtt
     1201 caaggcggat ttcgtttcct cgataaacca cgacatacgg tcctttctcg ttcccgtttc
     1261 aggtttcata ggcaggatcc ttgagggcaa gtacggccct ctggaagacg ccgtgaggga
     1321 aaggctcctg aatgtcgcgg acaacgtgtc gaaggttcac caccttgtcg aaaactacct
     1381 gaacgtgtcc aggatcgaat cgggaaagct cgacctcgcc atcggccccg tcgacatgaa
     1441 cgaggtcatt cgcgacgtgg cgaagctcta tgcgcccaga gtgatcttcg ccgacggcgc
     1501 aaccccgcag gccctcgttc ttgcggacaa ggactacatc gaacgggtca tcacgaacct
     1561 tgtcgtcaac gccctgaagt tctcgcccga agattccacg gtcaggatag ggacccgcgt
     1621 cgagggcggc atgctcgtga ccttcgttga ggacaggggc gcggggatac ccgcacggga
     1681 acttcgcttc atcttcgaga agtaccgccg cggaagcctg gggagacggg aagagggcag
     1741 cggactgggc ctgttcatcg tgaaatccgt cgtggaagga cacggagggt ccatctgggt
     1801 cgatagcgtc cccggcaggg gaagcacctt cttcttttcc cttcccctct ccaggggcga
     1861 gatataaggt ttgacaaagc tcaacattta aaaatttgac ttttcgcagt tacatattta
     1921 taatcactcg atgcgtaaaa tcgtaattgt tttactggca gtcgttctcg cttcctgcag
     1981 ctacatgccc tggggtaaaa agaaagacga ggacccctcc aagactacga agaagaccga
     2041 ttcggccgcc cagaaaaaac ctgctcgagg cgagccggag gaaatgaaag agcccgaact
     2101 gggcgacatc aaggtcatcg acggcgtgga atacatctac acgcggaaca ggaaatacat
     2161 gctgacgccc tacgagccgc cctacgtctg ggtccgcaag gaccagtacg ccccgggcat
     2221 cggggaggcg ctttccttcg gcatcggcgg cagcggtccc agcaagaa
//
LOCUS       DAOPOA010000395         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_51748_length_2265_cov_5.362443, whole genome shotgun sequence.
ACCESSION   DAOPOA010000395 DAOPOA010000000
VERSION     DAOPOA010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51748_length_2265_cov_5.362443"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1277
                     /locus_tag="PKJ17_08320"
     CDS             360..1277
                     /locus_tag="PKJ17_08320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOD76018.1"
                     /translation="MPKHFYTLLIIPHKKKDSVKKFLATPHHFRLATALSIVLFCFLG
                     YCAVDYLTIKLEQMELFNLRQLTSTQQEQIDTLEGKITFFERKLAELKQVDEKIRTMA
                     SDLTGKSRKASRKEAAPAREQALGVGGPMPAGEAGADKLTNLNRHMDRLLEDASARER
                     SLAELQEFLRAQRSIAAVTPNLWPVTGWVTSEFGPRANPFGSRREFHSGMDIATKLGA
                     PIQAPADGIVANVEKRPDLGLLIKVEHGRGIHTLYAHLFRSAVSKGQVVKRGEVLGYV
                     GNSGRSTGAHLHYSVNLNGVYVNPRKYLP"
     gene            1354..>2265
                     /locus_tag="PKJ17_08325"
     CDS             1354..>2265
                     /locus_tag="PKJ17_08325"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76019.1"
                     /translation="MLNFIQKLFGTKNERELKRIAPLVDEINGFEDGVRSLSDGQLKA
                     KTAEFKEKLADGMTLDDILTEAFAVAREAARRTVGMRPFDVQLIGGIVLHEGKIAEMK
                     TGEGKTLAATMPLYLNALTGKGCHLVTVNDYLARRDAAWMGPIYNFLDLSVGVIVHGL
                     TDAERRAAYGSDITYGTNNEFGFDYLRDNMKFTLEDYVQRDFHYAIVDEVDSILIDEA
                     RTPLIISGPSDESTDKYYRINQIIPGLRRERDYTIDEKARAVVLTEEGVARVEKALKV
                     QNLYDPRNIELLHHVNQALKAHTLFKRD"
BASE COUNT          480 a          704 c          678 g          403 t
ORIGIN      
        1 agacccgctg ctgtcggtca aaaaagcccc cgaaggagag gtgggaagcc acggcgcccg
       61 ccggggaaag ggctgatttt tgacgtcgag gcatccacat acgcttgaat tcgtttcatt
      121 tctcttgaaa ctggcgattt cgtcttgaca ggccgcccgt ttgcggatat ttaggacgcc
      181 tgtctccgga atccggctgt tttaccggtt cgggaccggg gcgtgttttc gacgcggtga
      241 tttgggggat gcagagtgtc cctcggcatc acgcgacgtc tttcctcccg tacttgtcag
      301 gggaagcggg aagtctcttt tcccggctcc cgggtacaaa gaaaggatga ggatgagcaa
      361 tgccgaagca tttttacacc ctcctgatca tcccgcataa aaagaaagac tccgtaaaga
      421 agttcctcgc cacgccgcat catttccgcc tcgccacggc cctgtcgata gtgctgttct
      481 gttttctcgg atactgcgcc gtcgattacc tgacgatcaa gctcgagcag atggaactct
      541 ttaatctccg ccagctcacg tcgacccagc aggagcagat cgacaccctc gaggggaaga
      601 tcaccttctt cgagaggaag ctggccgagc tcaagcaggt ggatgaaaag atccgcacca
      661 tggcgtctga tctgaccggc aagtcccgca aggcttccag gaaggaagcg gcgcctgccc
      721 gggaacaggc tctcggcgtg ggtggcccga tgccggccgg ggaggccggc gccgacaagc
      781 tgaccaacct caaccggcac atggaccgtc tgctggagga tgcgtcagcc cgcgagcgaa
      841 gccttgccga gctgcaggaa ttcctgcgcg cgcagcgctc catcgccgcc gtgacaccca
      901 acctgtggcc cgtcaccggc tgggtgacct ccgagttcgg ccccagggcc aaccccttcg
      961 gcagccggcg ggagttccat tcggggatgg acattgcgac gaagctcgga gcccccatcc
     1021 aggcgccggc cgacgggatc gtggccaacg tggagaagag accggacctg ggcctcctca
     1081 tcaaggtcga acatggaagg ggcatccata ctctctatgc ccacctcttc cgctccgcgg
     1141 tcagcaaggg acaggtggtc aaacgcgggg aggtcctcgg gtacgtcggc aactcaggac
     1201 gcagcaccgg ggcgcacctg cactattccg tgaatctcaa tggagtctat gtcaacccca
     1261 ggaagtatct gccctgattc ccttttgcga aaaaacaggg tatattgtcc gaaaatccgg
     1321 taagggctta ccgggttttt tttaggcgga accatgctga atttcatcca gaagctgttc
     1381 gggacgaaga acgaaagaga actgaagcgg atcgcgcccc tcgtggacga gatcaacggc
     1441 ttcgaggacg gcgtccgcag cctttccgac gggcagctga aggccaagac ggccgagttc
     1501 aaggagaaac tcgcggacgg catgaccctc gacgacatcc tgacggaagc cttcgccgtg
     1561 gcgcgggagg cggcccggag gaccgtcggg atgcggccct tcgacgtgca gctcatcggg
     1621 ggcatcgtcc ttcacgaggg caagatcgcg gagatgaaga cgggcgaagg gaagaccctc
     1681 gcggcgacca tgcccctgta cctcaacgcc ctgaccggca agggctgcca tctcgtcacg
     1741 gtgaacgact acctggcgcg ccgcgacgcc gcctggatgg gccccatcta caacttcctg
     1801 gacctttccg tcggtgtcat cgtccacggc ctgaccgacg cggaacggcg cgccgcctac
     1861 ggctccgaca tcacctacgg gacgaacaac gagttcggat tcgattacct gcgcgacaac
     1921 atgaagttca ccctcgagga ctatgtccag agggacttcc actatgccat cgtcgacgag
     1981 gtggacagca tcctcatcga cgaggcgaga acgccactca tcatctcggg tccctccgac
     2041 gaatccacgg acaagtatta ccggatcaac cagatcattc ccgggctccg gcgcgagagg
     2101 gactacacga tcgacgagaa ggcgcgggcc gtcgtcctca ccgaggaagg tgtggcccgg
     2161 gtggaaaagg ccctgaaggt gcagaacctc tatgacccgc gcaatatcga gctgctgcac
     2221 cacgtcaacc aggccctcaa ggcccacacc ctcttcaagc gtgac
//
LOCUS       DAOPOA010000396         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_51922_length_2259_cov_3.958711, whole genome shotgun sequence.
ACCESSION   DAOPOA010000396 DAOPOA010000000
VERSION     DAOPOA010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51922_length_2259_cov_3.958711"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PKJ17_08330"
     CDS             <1..1044
                     /locus_tag="PKJ17_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOD76020.1"
                     /translation="NPYQDNGIKIFSGSGFKLSDEQEEAIEELMMSNTLHGLVPPVRE
                     MGQAFRLEDAHGRYIVFVKNSFPRDLSIEGMKIVLDTANGATYKVAPDTFWELGADIE
                     VIHNTPNGININDRCGSQHTEDLRKRVVESGAAVGLAFDGDGDRLIAVDEKGREMTGD
                     QILLICARMLKEQGKLKNDLLVSTVMSNLGLRVACRKYGFRYHASKVGDRYVLEEMMR
                     LGGVVGGEDSGHMIFLDHHTAGDGIITALQLVAAMVRTGKPLSELAKWMDIYPQKLIN
                     VDVRTKPDIATIPQVMAVIDGVEKELGDEGRVLVRYSGTQNMCRVMVEGPSDAVTEKH
                     CTEIADVIRAHLG"
     gene            1066..2001
                     /locus_tag="PKJ17_08335"
     CDS             1066..2001
                     /locus_tag="PKJ17_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76021.1"
                     /translation="MARKIKEMSGIEEAIVGLIGARGPLTGFEIQKEIRTDSLLLWRA
                     CRTSGRLEVRSVGTRYMRLDRHVDGFARLSPSILREFLTYCVIGLPDDSSALDDRCLA
                     VGDRIREISRLKFDRALQMVLDVMEEYEDAAEMAGRMCFIIAGDIVYNMAHDVPRPER
                     STGKLVKGSDIDLVVVTDDDVADGPIGRLDELIYRRKYRMLIDPAVNEEVDYKIKRLM
                     RVREQAAFDDFKRMVAVKILDEGVFLHGSEEIFRAVKDIIRERGLDGRLAELEARAHV
                     FRSRAEMSILDGSLNGEKIKETHLFYSAEEFEEFE"
     gene            2038..>2259
                     /locus_tag="PKJ17_08340"
     CDS             2038..>2259
                     /locus_tag="PKJ17_08340"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HOD76022.1"
                     /translation="MLIGIDVGGTYTDGILFSEGSVLLSVKHPTDENDLEGTLLSVLD
                     ELLARGDAKDVQRIVLSTTLVTNLLATGRG"
BASE COUNT          494 a          636 c          728 g          401 t
ORIGIN      
        1 aatccctatc aggacaacgg gataaagatc ttttccggca gcggcttcaa gctctccgac
       61 gaacaggagg aggccatcga ggagctgatg atgagcaata cgcttcacgg cctcgtgccc
      121 cccgtccggg aaatggggca ggccttccgc ctcgaggacg cgcacggcag atacatcgtt
      181 ttcgtgaaga acagctttcc ccgggacctc tccatcgagg ggatgaagat cgtcctcgat
      241 accgccaacg gcgccacgta caaggttgcc cccgatacat tctgggaact gggcgccgac
      301 atcgaggtca tccacaatac gccgaacggc atcaacatca acgacaggtg cggttcgcag
      361 catacggagg acctcagaaa gagggtcgtg gaaagcggcg cggctgtcgg gctcgccttc
      421 gacggggacg gcgaccgcct catcgccgtc gatgagaaag ggcgggagat gacgggcgac
      481 cagatccttc tcatctgcgc gaggatgctc aaggagcagg gtaaactgaa gaacgacctt
      541 ctcgtgagca ccgtgatgag taaccttggg ctaagggtcg cctgcaggaa atacggcttc
      601 aggtaccacg cgtcaaaggt cggcgaccgc tacgtcctcg aggagatgat gcgcctcggc
      661 ggcgtggtgg gtggcgagga ttcggggcac atgatctttc tcgaccatca cactgcgggt
      721 gacggtatca tcaccgccct ccagctcgtc gccgccatgg tcagaacggg caagcccctg
      781 tcggagctgg cgaaatggat ggacatctat ccccagaagc tcatcaatgt cgatgtcagg
      841 accaagcccg atatcgccac catacctcag gtgatggcgg tgatagatgg ggtcgaaaag
      901 gaactcggcg acgaggggcg tgtcctggtg cggtattccg gtacccagaa catgtgcagg
      961 gtgatggtcg aagggccgtc agacgcggtc acggagaagc actgtaccga gatcgcggat
     1021 gtgatcaggg cccatctcgg ctagagaagc gggagcttcc cgcgcgtggc gcggaagatc
     1081 aaggaaatga gcggtattga agaagcaatt gtgggtctca tcggcgcgag aggccccctg
     1141 acgggttttg agatccagaa ggagatacgg acggacagcc tgctcctgtg gagggcttgc
     1201 aggacctccg gcagactgga ggtccgctcc gtgggcacgc gatacatgag gctcgatcgc
     1261 catgtcgacg gttttgcccg cctttccccg tcgatcctcc gggagttcct cacctactgc
     1321 gtcatcggcc tgccggatga cagttccgcc ctcgacgaca ggtgcctcgc ggtgggcgac
     1381 cggatccggg agataagcag gctcaagttc gaccgtgccc tccagatggt cctcgatgtg
     1441 atggaggaat acgaagacgc cgcggagatg gctggccgga tgtgtttcat catcgccggg
     1501 gacatcgtgt acaacatggc ccacgatgtg ccccgtccgg agcgcagcac gggtaagctc
     1561 gtcaaggggt ccgacatcga ccttgtggtc gttaccgatg acgacgtggc ggacggcccc
     1621 ataggtaggc tcgatgagct gatataccgc agaaagtaca ggatgctcat cgaccccgcc
     1681 gtgaacgagg aagtggacta caagataaag aggctcatgc gtgtcaggga gcaggcggcc
     1741 ttcgacgact tcaagcgcat ggtcgccgtc aagatcctcg acgagggagt ttttctccac
     1801 gggagcgagg agatcttccg cgccgtcaag gacatcattc gtgagagggg cctcgacggc
     1861 cgcctcgccg aactggaagc ccgggcacac gtgttcaggt ccagggccga gatgtcaatt
     1921 ctcgacggca gcctcaacgg ggaaaagata aaggagaccc accttttcta ctccgcggag
     1981 gagttcgaag aatttgaatg accccggacg gccggggaaa gataagaggt gaacaacatg
     2041 ctgatcggta tcgatgtggg cggcacatac acggacggga tccttttttc ggaagggtcg
     2101 gtccttttga gcgtcaagca ccctacggac gagaacgatc tcgagggcac ccttctgtcg
     2161 gtgctggacg agctcctcgc gcgcggggat gcgaaggacg tgcaaaggat agtcctctcc
     2221 accaccctcg tcaccaatct cctcgccacg ggacggggg
//
LOCUS       DAOPOA010000397         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_52365_length_2245_cov_2.969863, whole genome shotgun sequence.
ACCESSION   DAOPOA010000397 DAOPOA010000000
VERSION     DAOPOA010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52365_length_2245_cov_2.969863"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PKJ17_08345"
     CDS             <1..964
                     /locus_tag="PKJ17_08345"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOD76023.1"
                     /translation="DKAIEVVGTAVDGRDALEKVESLKPDVITMDVEMPVMNGIEALK
                     KIMEKDPVPVIIMSALTREGAEITMEALHLGACDFVTKDFTNLGNSFSSKEAEVIAKV
                     KNVAKNKVRFLLRKLDIKPRPAAVISPDQKVRHEVLAIGASTGGPPALQHILTRLPRA
                     FPVPIVIAQHMPKIFTQSFSQRLDAVSQLQVKEAEDGETLRAGIAYIAPGNTHMAVRR
                     KGRSAFVQFTDGTQYIYRPSVDLLMSTTAETFERQSFGVILTGMGNDGVQGMKEMKAK
                     GGYIVAQDEETCVVFGMPRAVISANLADAVLPIDKISEEIMRVL"
     gene            961..>2245
                     /locus_tag="PKJ17_08350"
     CDS             961..>2245
                     /locus_tag="PKJ17_08350"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOD76024.1"
                     /translation="MRTQEREDVRMQGLGGEELEHFITLLRDGDNFEKGKAIEALVES
                     PGKEVVERIVPLLQEKNTPVRMAVLDVLKRIGSVHLDGVIGMLEDENEDIRVYACEVL
                     SFLRDPRSIPFIVKKSRDDVDNVRNAACMALGEFDDDRAVMALLDALKDEEWIAFSAI
                     LSLGRAKSPKAVPRLLQFFRESGEELSDAACEVLVEYGDDAVLDELFDVLKGWDREKR
                     SAYLKIILEKGDEGTFGRLKEKIGDELYEHLLGSAAENRHRPVEIVRMLTHFRMPETC
                     GAILDILKEMDPDDENYEMVLTLFASLSEVWAADAGGYMERDEANLLPLVRACKMTGV
                     KVGEAALLRHFLTAPVEVKREIVMSVPAVVDGSGCSIIKEALKDTDGHIKGFAVDAVG
                     TLKLTNLKEDIVRILREDFHDVRVKALKTLIRLDPT"
BASE COUNT          505 a          623 c          713 g          404 t
ORIGIN      
        1 agacaaggcc atcgaggtcg tgggcaccgc cgttgacggc agggacgccc ttgagaaggt
       61 ggagtccctg aaacccgacg ttataaccat ggacgtggag atgcccgtca tgaacggcat
      121 cgaggccctg aagaagatca tggagaagga cccggtgccg gtgatcatca tgagtgccct
      181 gaccagggaa ggggccgaga ttaccatgga agccctgcat ctcggtgcct gtgatttcgt
      241 gaccaaggac ttcacgaatc tgggcaactc cttctcgtcg aaggaggcgg aagtgatcgc
      301 gaaggtgaag aacgtggcaa agaacaaggt caggttcctc cttcgcaagc tggatatcaa
      361 gcccagaccc gccgccgtga tcagcccgga ccagaaggtc cgtcacgagg tccttgccat
      421 aggcgcctcc acgggcgggc cgccggccct gcagcacatc ctgacgaggc tcccccgcgc
      481 cttcccggta cccatcgtca tcgcccagca catgcccaag atcttcaccc agtccttttc
      541 ccagagactc gacgccgtgt cgcagctgca ggtgaaggaa gccgaggacg gggagaccct
      601 gcgggcgggc atcgcctaca tcgcccccgg gaacacccat atggcggtga ggagaaaggg
      661 cagaagcgcc tttgttcagt tcacggacgg gacacagtac atctatcggc cttccgtcga
      721 ccttctcatg agcaccacgg cggagacctt cgagagacag tctttcggtg tcatcctgac
      781 cggtatgggc aatgacgggg ttcagggcat gaaggagatg aaggccaaag gcggatacat
      841 cgtggcccag gacgaagaga catgcgtcgt cttcggcatg cccagggcgg tgatctcggc
      901 caacctggcc gacgccgtgc ttcccatcga caaaatatcg gaagagatca tgagggtgtt
      961 atgagaactc aggaaagaga ggatgttcgg atgcaggggc ttggcggtga agaactggaa
     1021 cacttcatca ctcttctcag ggacggcgac aatttcgaga agggaaaggc gatagaggcg
     1081 ctcgtcgagt ccccgggaaa ggaagttgtg gagcgcatag tcccccttct tcaggagaag
     1141 aacacgcctg tcagaatggc cgttctcgat gtcctgaagc ggatagggag cgttcatctc
     1201 gacggcgtca tcgggatgct cgaagacgag aacgaggaca tacgggtcta tgcctgcgag
     1261 gtcctctcct tcctcaggga cccgcgctcc ataccattca tcgtgaagaa gtcccgcgat
     1321 gacgtggaca acgtgaggaa cgcggcctgc atggcgctcg gcgagttcga tgacgaccgg
     1381 gccgtcatgg ccctccttga cgccctgaag gacgaggaat ggatagcctt ttccgccatc
     1441 ctgagcctgg gaagggcgaa gagcccgaag gccgtccccc ggctcctcca gttcttcagg
     1501 gaaagcggcg aggaactctc ggacgcggcc tgtgaagtcc tcgtggaata cggtgatgat
     1561 gccgtcctcg acgagctgtt cgatgtgctc aagggatggg acagggagaa aaggagcgcc
     1621 tacctcaaga tcatcctcga gaagggtgat gaggggacct ttgggaggct caaggagaag
     1681 attggggacg agctctacga gcatcttctc ggaagcgccg ccgagaaccg gcaccgtccc
     1741 gtcgagatcg tgcgcatgct gacccatttc aggatgcctg aaacatgcgg cgccattctc
     1801 gatatcctca aggagatgga ccccgacgac gagaactatg aaatggtgct cacgcttttt
     1861 gcttccttaa gcgaggtgtg ggcggcggat gccggcggat acatggagcg tgacgaggcg
     1921 aaccttctgc cccttgtcag ggcgtgcaag atgacgggtg tgaaggtcgg ggaggctgcc
     1981 ctcctgaggc acttcctgac agcacccgtg gaagtgaagc gcgagatcgt gatgagcgtt
     2041 ccggcggtcg tagatggaag cggatgctcc atcatcaagg aggccctcaa agacaccgac
     2101 ggccatatca agggttttgc cgtcgacgcg gtgggtactc tcaagctcac gaatctcaag
     2161 gaagacatcg tccggatcct cagggaggat tttcacgatg tgagggtcaa ggcgttgaag
     2221 accctcatca ggctcgatcc caccc
//
LOCUS       DAOPOA010000398         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_52526_length_2240_cov_3.166590, whole genome shotgun sequence.
ACCESSION   DAOPOA010000398 DAOPOA010000000
VERSION     DAOPOA010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52526_length_2240_cov_3.166590"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PKJ17_08355"
     CDS             complement(<1..1034)
                     /locus_tag="PKJ17_08355"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76025.1"
                     /translation="MAQRRTRRNDDKADLLLGEKGTIRKKWGGRIPVLIVFPNSYHVG
                     MSNLATHILYKTLNDRDDVVCERLFYEEGRPLVSLESSRPLSSFEIVFFTLSFELDYP
                     NIVKVLEGSSVEVLARERTEDSPLIVAGGICVIANPEPVSPFFDLMVMGDIEATISSL
                     MERFIAGRGPGRGRIVDELTALPFVYNPAELSVRYGEDGRVEGFEPPDFAVTVERHTG
                     KTLGMSVMTTPNTEFADMVLIEGTRGCPSRCTFCLLGNLYGFRSEDIDVTVGEAKDVG
                     IIGGGVSFHPRIAEIVGNLSSRGIAVHLPSLRLDEVPLRLIELMKDTVKTLTFGVEAA
                     TESLRHLLGKP"
     gene            complement(1295..>2240)
                     /gene="ftsZ"
                     /locus_tag="PKJ17_08360"
     CDS             complement(1295..>2240)
                     /gene="ftsZ"
                     /locus_tag="PKJ17_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOD76026.1"
                     /translation="GANPEVGKKAALEDVDKLKDHLKGAHMVFITCGLGGGTGTGASP
                     VIAEIAREVGALTVAIATKPFAFEGKDRMNQSEGGVVQLKSRVDSLITIPNQRLLSIG
                     GKHMTIKEAFLKADEVLLNAVRSISDLIVGSGHVVVDFADVKTIMSERGMAIMGIGES
                     EGENRARDAAQKAISSPLLEDISIHGARGVLINVTGNTDMTLMEVSEASTLIQEQAHE
                     DAKIIWGLVYDETMQDSLRITVIATGFEERAVIDEDRTEPFLRGKLFDDENVPSFMKK
                     KVAIDYKVDYKEIRQKSDTIDIDDDRYDIPTFLRKQAD"
BASE COUNT          407 a          651 c          691 g          491 t
ORIGIN      
        1 ggcttgccga ggaggtgtcg caggctttcc gttgctgcct cgacgccgaa ggtgagggtc
       61 ttcaccgtgt ccttcatgag ttcgatgagg cgtaagggga cctcgtcgag gcgaagcgac
      121 gggagatgga ccgctatccc ccgggaactc aggttcccga cgatctcggc gatgcgggga
      181 tggaaggaca cgccgccgcc tatgatgccc acgtctttcg cctctcccac cgtcacgtcg
      241 atgtcctccg atctgaagcc gtaaagattg ccgaggaggc agaaggtgca ccgcgaggga
      301 cagccgcggg tcccctcgat gaggaccatg tccgcgaact ccgtgttcgg agtggtcatg
      361 acggacatac ccagcgtttt tcccgtgtgg cgctccacgg tgacggcgaa gtcggggggc
      421 tcgaaacctt ccacccggcc atcttcaccg taccgtaccg acaactccgc ggggttgtaa
      481 acgaagggca gcgccgtgag ctcgtcgacg atacgacccc tccccggacc ccggcccgcg
      541 atgaagcgtt ccatgaggga ggatatagtg gcctcgatgt cgcccatcac catgagatcg
      601 aaaaagggcg acacgggctc ggggttcgcg atgacgcaga tcccgccggc gacgatcagc
      661 ggggagtcct ccgtccgctc ccgggcgagg acctcgactg atgacccttc aagtaccttc
      721 acgatattgg ggtagtcgag ttcaaaggaa agggtgaaga agacgatctc gaaggaggag
      781 aggggccggg agctctccag cgagaccagc ggtctgcctt cctcgtagaa gagccgttca
      841 cacaccacat cgtcgcggtc gttcagcgtc ttgtaaagga tatgggtcgc gaggttcgac
      901 atccccacgt gataggagtt ggggaaaacg atgagaacag gtatcctgcc cccccacttt
      961 ttccggatgg tacccttttc cccgagaaga aggtccgcct tgtcgtcatt ccgtcgcgtc
     1021 cgtctttgag ccattgcagt aataacaata acacagatca ggtgatgggt gataggtcat
     1081 aggtgacggg acggggcttg agtcgttgag agttaaagac gggagacgat ggtgcggtcc
     1141 ggcgctgtct tagctgccct aactttcttc tatggatctt atgatatttt gtcgctcaag
     1201 ccattcaagc ggctcaagcc attcaagccg tcttttggct gtgaggttga ggggtggcgg
     1261 gacgagagct ttcgccaccc ctcgcttcag gaggttagtc tgcctgcttc ctgaggaatg
     1321 tcggaatgtc gtagcggtca tcatcgatgt cgatggtgtc gctcttttgc cgtatctcct
     1381 tgtaatcgac cttgtagtcg atggccacct tcttcttcat gaaggaggga acgttctcat
     1441 cgtcgaagag cttgcctctc aagaacggtt ccgtcctgtc ctcgtcgatg acggcgcgtt
     1501 cctcgaagcc cgtggcgatg acggtgatcc tcagggaatc ctgcatcgtc tcgtcgtaga
     1561 cgaggcccca gatgatcttc gcatcctcgt gggcctgttc ctgtatgagg gttgaggcct
     1621 cggagacctc catcagcgtc atgtccgtgt tgcccgtgac gttgatgagt acgccgcgcg
     1681 ctccgtggat ggagatgtct tcgaggagcg ggctcgatat ggccttctgg gcggcatcgc
     1741 gggccctgtt ctcgccttcg gattcaccga tgcccatgat cgccatgccc cgctcgctca
     1801 tgatcgtctt cacgtccgcg aagtcgacga cgacgtgacc ggacccgacg atgaggtcgg
     1861 agatgctcct gaccgcgttg agcaggacct cgtcggcctt gaggaacgcc tccttgatcg
     1921 tcatgtgctt gccgccgatg gaaaggagcc tctggttggg gatggtgatg agcgagtcga
     1981 cgcgcgactt gagctggaca acgccgccct cggattggtt catcctgtcc tttccctcga
     2041 aggcgaaggg tttcgtggcg atggccacgg tgagggctcc cacctccctc gctatctcgg
     2101 cgataacggg ggatgcgccc gttcccgtcc caccgccgag gccgcatgtt ataaacacca
     2161 tgtgggcacc tttcaggtgg tccttgagct tgtccacatc ctcgagggcg gccttcttgc
     2221 ccacctctgg attggccccg
//
LOCUS       DAOPOA010000399         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_52589_length_2238_cov_3.174530, whole genome shotgun sequence.
ACCESSION   DAOPOA010000399 DAOPOA010000000
VERSION     DAOPOA010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52589_length_2238_cov_3.174530"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PKJ17_08365"
     CDS             <1..619
                     /locus_tag="PKJ17_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD76027.1"
                     /translation="ILASPSFLSSETLSRDGARGIISEAGEHHTLLIIDETLKGYSGT
                     PSLAADILQREGCVVMGSLGEYYGLAGLSVSYVIGTAAVIEDMRRRKAVTPPNTLAAA
                     AATASLRDPTFSARTRTFMERERSFIAEGLRGIPGISFSSSACGSFIVTLVDDPSKPL
                     ETFEHYRIVVDDLGDAGTLFFPVKDHKWNARYLKTLKNIMGTPKQ"
     gene            616..2190
                     /locus_tag="PKJ17_08370"
     CDS             616..2190
                     /locus_tag="PKJ17_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOD76028.1"
                     /translation="MKRTTTSTLFLLISLVIFLFTLHLAPACAASLRILHVNDFHGFA
                     ESHKRLGSDDVRGGVACLAYLAETLRKEKPSLLLAAGDMIQGSTWSNLFEGRSVIEVM
                     NVMRFDAMVVGNHEFDFGKTVLTELTREADFPVLGANVTGLDALKPYIVKEVDGIRVG
                     IIGVVTEETPTATHPRNVEGLTFMPAAKTVERCIGELKGRADIIVVLSHLGYNADMAL
                     ASTVKGIDVIVGGHSHTKGVKPITVGSTVIVQAWEHGLVLGVLDLTVESGRIVAVEGR
                     LEDIVPEKMARDEKVAAIVEKWAGKVRESMGKVIAEAEVDLDGENVRVRETNLGNFIA
                     DILKKESGADAAIINGGGLRTSIKKGPVTVGSIYSVMPFNNYIVGIRLTGQQIRDTLE
                     YGLSGIESKEGRFPQVAGIAVTYSPGGEPGKRVRTIHVAGEPIDLSRRYIVATVDFLA
                     AGGDGYQVFREAIRQSPDFQILGGTIKSGNLVYNDAGRWVRDAVVSEAARQKKLAPVV
                     DGRIKAEGSGDEGPRK"
BASE COUNT          474 a          668 c          689 g          407 t
ORIGIN      
        1 catccttgca tccccctctt ttctgtcctc ggagacacta tcccgcgacg gggctcgcgg
       61 gatcatttca gaggccgggg aacaccatac ccttctcatc atcgacgaaa ccctgaaagg
      121 atactccggc acaccctcgc tcgctgccga catcctgcag agggagggat gcgtcgtcat
      181 ggggtccctt ggcgagtatt acggcctcgc cggcctgtcc gtctcctacg tcatcggaac
      241 ggccgccgtg atcgaggaca tgaggcggcg aaaagcggtg actcccccca acacactcgc
      301 cgccgcggcg gcgacggcgt cactgcgcga cccgacgttc tccgcgcgga cgaggacgtt
      361 catggaacgg gagagatcct tcatcgccga gggtcttcgg ggtatcccgg gaatttcctt
      421 ctccagctct gcctgcggtt ctttcatcgt gaccctcgtc gacgacccgt caaaacccct
      481 cgagaccttc gaacactaca ggatcgtcgt cgacgacctg ggagacgcag ggaccctgtt
      541 ctttcccgtc aaagaccaca agtggaacgc gcgttacctg aaaactctga agaacattat
      601 gggaacaccg aaacaatgaa aagaacaacg acctcgacac tattcctgct tatatccctc
      661 gtcatctttc tcttcaccct gcacctcgcg cctgcctgcg ccgcttcctt gcgtatcctc
      721 cacgtgaacg atttccatgg cttcgccgag agccacaagc gcctggggtc cgacgacgtt
      781 cgcgggggcg tcgcctgcct cgcctatctc gcggagaccc tgaggaagga aaagccttcc
      841 ctcctccttg ccgccggtga catgatacag ggcagcacct ggtcgaacct cttcgaggga
      901 aggtccgtca tagaggtgat gaacgtcatg cgtttcgacg ccatggtcgt cggcaaccat
      961 gaattcgatt tcggcaagac ggtgctcacg gaattgacca gagaggcgga tttccccgtc
     1021 ctcggggcca acgtgaccgg cctcgacgcg ctcaagcctt acatcgtcaa ggaggtggac
     1081 ggcatcaggg tgggtatcat cggtgtcgtc acagaggaga cccccacggc cacgcacccc
     1141 cggaatgtcg aggggttgac cttcatgccc gcagcgaaga ccgtcgaacg ctgcatcggc
     1201 gagttgaagg gcagggccga catcatcgtg gtcctctcgc acctcgggta caatgccgac
     1261 atggccctcg cgtcgactgt caagggcatc gatgtcatcg tgggcggaca ttcccacacg
     1321 aagggagtga agccgatcac ggtgggcagc acggtgatcg tccaggcctg ggagcatggt
     1381 ttggtattgg gggtcctcga cctcacggtg gagtcgggca ggatcgtcgc cgtggaaggc
     1441 aggctcgagg atatcgtgcc cgagaagatg gcaagggacg aaaaggtcgc cgcgattgtc
     1501 gagaagtggg ccgggaaggt tagagaatcc atggggaagg tgatcgccga ggccgaggtc
     1561 gatctcgatg gagagaacgt gcgcgtgagg gagaccaatc tcggcaattt catcgccgac
     1621 atcctcaaaa aggagtccgg cgccgatgcc gccatcatca acggaggcgg gctccgtacc
     1681 agtataaaga agggtcccgt cacggtcgga agcatctatt ccgtcatgcc tttcaacaac
     1741 tacatagtgg gcatccggct gacggggcag cagatcaggg acactctcga atacgggctc
     1801 tcgggtattg aatcgaagga aggacgcttt ccgcaggtgg cgggtattgc cgtcacgtat
     1861 tcccccggag gggaaccggg caagagggtg agaacgatcc acgtcgccgg ggaacccatc
     1921 gacctctcac gacgatacat cgtggccaca gtggacttcc tggcggcagg aggggacggg
     1981 taccaggtct tccgcgaggc catcaggcaa tcgccggatt tccagatcct gggcggaacg
     2041 attaagagcg ggaacctcgt ttacaacgac gccggcagat gggtgaggga cgcggtcgta
     2101 tcggaggcgg cgcggcaaaa gaaactggcc cccgtggtgg acgggaggat aaaggccgaa
     2161 ggatcaggcg atgaaggtcc tcgtaaatga cagggagatc catctcgccc cgggtatgac
     2221 ggtacgccac gccctcat
//
LOCUS       DAOPOA010000400         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_52628_length_2237_cov_2.931714, whole genome shotgun sequence.
ACCESSION   DAOPOA010000400 DAOPOA010000000
VERSION     DAOPOA010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52628_length_2237_cov_2.931714"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..85
                     /gene="ffs"
                     /locus_tag="PKJ17_08375"
     ncRNA           <1..85
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKJ17_08375"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            125..1714
                     /gene="dnaX"
                     /locus_tag="PKJ17_08380"
     CDS             125..1714
                     /gene="dnaX"
                     /locus_tag="PKJ17_08380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOD76029.1"
                     /translation="MSYTVIARRWRPQRFDDVVGQPHIITTLKNSITAGRIAHAFLFT
                     GPRGVGKTSLARILAKAVNCVNGPTAEPCNVCENCVGIAGGNFVDVIEIDAASTRGID
                     DIKELRETVRYLPMKAAYKVYILDEAHMLTDQARNAFLKTLEEPPGHNIFILATTELQ
                     KIPYTIMSRCQRFDFRRITEKEIVEQLKAICKKDGITFDEGAFHHVAIEADGSMRDAE
                     SILEQIIAYSGQHISEGDVINIIGVVEKEVVHRIVRSLLTKDLNDGLSAIETTLGDGY
                     DVNQVYRGLVSYLRNMMIVKAVGGLPDFISMGEDEYKALSGLIANIEYYEIQNMLYYM
                     LRSEDLMRGLFPRIALETLYINLYNLSQLRDVEKVIGELQRGEPQASRAGERTQGTGY
                     RERDTGSRERDMGDGRQEAEGDVSRAPSAPSREENASAAGGRPAGEGVRGFVEYLKKK
                     KPFLGSMFDSCECSVEGDDLIVLVDKKYGNFIKSEFEEVRKLASEFFGRAMNAEFRDA
                     GDRKKNILEEYVKEADSLFNL"
     gene            1726..2040
                     /locus_tag="PKJ17_08385"
     CDS             1726..2040
                     /locus_tag="PKJ17_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOD76030.1"
                     /translation="MSKGFNQILQQAKKMQDRLMKLQEEMADKTVEAQSGGGMVSCVV
                     NGKQEVVSLKISPEVLEEKDNELLEDLIVAAVNEGLDRSREMVQEEMSKVTGGMQMPF
                     GM"
     gene            2044..>2237
                     /locus_tag="PKJ17_08390"
     CDS             2044..>2237
                     /locus_tag="PKJ17_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HOD76031.1"
                     /translation="MFYPEPIERLIENLTRLPGIGRKTATRLAFFLLNAKEGYITELS
                     RSLVDIKEKIRLCSVCFNIT"
BASE COUNT          538 a          595 c          693 g          411 t
ORIGIN      
        1 gcaacgtgga ggttatgaac cccgtcaggt ccggaaggaa gcagcggtag tagctcgacc
       61 gtgtgccgca ggtagcctgg ctttttttgt tacacaccac gaacccccga agaggcgttc
      121 acatttgagc tacacggtca tagcaagacg ttggagaccc cagagattcg atgatgtggt
      181 cggtcagccg cacatcatta ccaccctcaa gaattccata acggcaggca ggatagcaca
      241 cgcctttctt ttcacggggc cccgtggcgt tggaaagacc tcgctggccc ggatcctcgc
      301 aaaggcggtc aactgcgtga acggcccgac ggctgagccc tgcaatgtct gcgagaactg
      361 cgttgggata gcgggcggca atttcgtgga cgtcatcgag atagacgccg cttcgacgcg
      421 ggggatcgat gacatcaagg aactgcggga gaccgtgcgc taccttccca tgaaggcagc
      481 ctacaaggtc tacatcctcg atgaggccca catgctgacg gaccaggcgc gcaacgcctt
      541 cctcaagacc ctggaggagc ccccgggaca caacatcttc atccttgcga ccacggaact
      601 gcagaagatt ccctacacga tcatgtcccg ctgccagcgc ttcgatttca ggcgcatcac
      661 ggaaaaggag atagtggagc agctcaaggc catctgcaaa aaggacggga taactttcga
      721 cgagggggcc ttccatcatg tcgcgatcga ggcggacggt tccatgcggg acgccgaatc
      781 catccttgag cagataatag catacagcgg gcagcacatc tccgaaggtg atgtcatcaa
      841 catcatcggg gttgtcgaga aggaggtcgt ccatcgcatc gtgaggtctc ttctcacgaa
      901 ggacctgaac gacggacttt cggcgatcga gacgaccctc ggcgatggct acgacgtgaa
      961 ccaggtctat cgcggtctcg tctcctacct caggaacatg atgatcgtga aggccgtggg
     1021 gggactgccc gatttcatct ccatgggcga ggacgagtac aaggccttga gtggtctcat
     1081 cgcgaacata gaatattacg agatccagaa catgctctac tacatgctcc gcagcgaaga
     1141 cctcatgagg gggcttttcc cgcggatcgc gctggagact ctctacatca acctctacaa
     1201 cctgtcgcag ctcagggacg tggaaaaggt cataggagag ctgcaacggg gagagccgca
     1261 ggcttcgcgg gcaggggaga ggacacaggg aacgggatac agggaacggg atacagggag
     1321 cagggaacgg gatatgggag acggcaggca ggaagcggag ggtgatgttt cccgtgctcc
     1381 gtccgcgccg tcccgtgagg agaatgcttc agcggcgggc gggcgcccgg cgggggaggg
     1441 cgtccgtggc ttcgtggaat acctcaagaa gaagaagcct tttctcggaa gcatgttcga
     1501 ctcctgcgaa tgttcggttg agggggatga tctcatcgtc ctcgtcgaca agaagtacgg
     1561 caatttcata aaaagcgaat ttgaagaggt ccgcaagctc gcgagcgagt tcttcggcag
     1621 ggcgatgaac gcagagttca gggacgccgg cgacaggaag aagaacatcc tcgaggagta
     1681 cgtgaaggag gcggattctc tcttcaatct ttaggaggag acagcatgtc caagggtttc
     1741 aaccagatat tgcagcaggc caagaagatg caggaccgtc tcatgaagct ccaggaagag
     1801 atggccgaca agacggtgga ggcacagtcc ggtggcggta tggtgagctg tgtcgtcaac
     1861 ggcaagcagg aggttgtctc tctgaagata tccccggaag ttctcgagga aaaggacaac
     1921 gaactcctgg aagacctcat tgtcgcggcc gtcaacgaag ggctcgaccg ctcccgcgag
     1981 atggtccagg aggagatgtc gaaggtgacg ggcggcatgc agatgccctt cgggatgtag
     2041 gacgtgttct accccgaacc gatcgaacgg cttatcgaga acctgacccg gctccccggc
     2101 atcggccgga agacggcgac acggctcgcc ttttttctcc tcaatgcgaa agagggatac
     2161 atcacggaat tgtcgagaag cctcgtcgat atcaaggaaa agatacgcct ttgcagcgtc
     2221 tgcttcaaca tcaccga
//
LOCUS       DAOPOA010000401         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_52630_length_2237_cov_2.883135, whole genome shotgun sequence.
ACCESSION   DAOPOA010000401 DAOPOA010000000
VERSION     DAOPOA010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52630_length_2237_cov_2.883135"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PKJ17_08395"
     CDS             <1..945
                     /locus_tag="PKJ17_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76032.1"
                     /translation="EALGRLRSRANPSARFDYSYWNEEGRGDLARITRNRFDVTVDVP
                     VRCQYHVFIKGHRWLDTPDFDRATYGAEGVSVGFAGVFSPFVKAEGSWTHRRYDSDTL
                     TDRDTGHGTVWFNLDDRVRLGAGYARTEEIYNYFGIAQGTQADRVFVALHSDVTRKLE
                     VSGKAEYIGYNDSNSGSLFTFAAGYALSDHPRTFKVAASGEMRNTRHDNEYVYRNGEL
                     VDIIHPYWAPRDYLAGSVLLEWRHDLSKTFICGAEQHFYDLKASFGTDSENNPYAKIE
                     GEWSWEFLKHWLVGIKALAHTSPKWDATGAWAWLRYRF"
     gene            946..1962
                     /locus_tag="PKJ17_08400"
     CDS             946..1962
                     /locus_tag="PKJ17_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD76033.1"
                     /translation="MTEKRPTSSRMVLVMAAPVAAMAFAAIYLIVRVILFMITDHLWY
                     EKALAFLLLMAEIFILVHGIGYFLEILSVAWTRREFARKELPVEPLSSYPPVAIVMPS
                     YKEPLEVVKSSLITFYNLSYPNKYIYFLDDTRYDVPWDTPENMEAYKRSIEELCQYVG
                     VDLFRRKWHGAKAGIINDFMEYLDGQTREGFVFHNYSKKIKKETERYMVVFDADSNPF
                     PDFLEPLVARMEDNPELAFIQTPQYYINFDSNRIARAAGLQQTVFYEYICEGKASKDA
                     TFCCGTNVLFRREALKSVGGFDESSVTEDFATSLQFHLNKWKTTYINRVRTFQIGPED
                     LGGY"
BASE COUNT          471 a          714 c          587 g          465 t
ORIGIN      
        1 gaggccctcg ggagacttcg gtcccgcgcc aatccctcgg cgcgtttcga ttactcctac
       61 tggaacgagg aaggcagggg cgatctcgcc cgcatcacgc gaaaccgctt cgatgtcacc
      121 gtcgatgtgc ccgtccggtg ccagtatcac gtctttatca agggccaccg ctggctggac
      181 acgcccgact tcgatcgcgc gacctatggc gccgagggtg tctccgtggg ctttgccggc
      241 gtcttcagcc ccttcgtgaa ggcagagggc tcctggacgc acaggagata cgactcggat
      301 acactgaccg acagggacac gggccacggg acggtctggt tcaatcttga tgaccgggtg
      361 cgcctcggcg ccgggtacgc ccgcacggag gagatataca actattttgg catagctcag
      421 ggcacgcagg cggaccgcgt cttcgtcgcc cttcacagcg acgtcacccg caagctcgag
      481 gtaagcggca aggcggagta cataggttac aacgactcca acagcggcag tctatttacc
      541 ttcgcggcgg ggtatgccct ttccgaccac ccgcggacat tcaaggtggc cgcctccggc
      601 gagatgcgca atacacgcca tgacaacgag tacgtctacc gcaacgggga gctcgtcgac
      661 ataatccacc cctactgggc gccgcgcgat tatctcgccg gcagcgtcct cctcgaatgg
      721 cggcacgacc tctcgaagac cttcatctgc ggggcggagc agcatttcta cgatctcaag
      781 gcctccttcg gcacggactc ggagaacaat ccctacgcga agatcgaggg tgaatggagc
      841 tgggagttcc tgaagcactg gcttgttggt ataaaggccc tggcccacac gtccccgaaa
      901 tgggacgcca cgggcgcatg ggcgtggctg aggtaccggt tctagatgac agaaaagcgc
      961 ccgacatctt caagaatggt tctcgtcatg gcggcacccg tcgccgcgat ggccttcgcc
     1021 gccatctacc tcatcgtgcg cgtcattctc ttcatgatca ccgatcacct gtggtacgag
     1081 aaggccctcg cgttcctcct cctcatggcg gagatattca tcctcgtcca cggcatcggg
     1141 tactttcttg agatcctctc cgtggcatgg acacgcaggg agttcgcccg aaaggaactc
     1201 cccgtggaac ccctgtcatc ctatccgccc gtggccatag tcatgccctc gtacaaagag
     1261 cctctcgaag tcgtcaagag ctctctcatc accttctaca acctctcgta cccgaacaag
     1321 tacatctatt tcttggacga tacccgatat gacgtcccct gggacacccc cgagaacatg
     1381 gaggcataca agagatccat cgaggaactt tgccagtacg tcggcgtcga cctcttccgc
     1441 aggaaatggc acggggcgaa ggcgggtatc atcaacgatt tcatggaata ccttgatgga
     1501 cagacgaggg agggattcgt attccacaat tattccaaga agatcaagaa agaaacggaa
     1561 cggtatatgg tcgtcttcga tgccgactcc aatccattcc ccgacttcct ggaacccctg
     1621 gtcgcccgga tggaggacaa ccccgaattg gccttcatcc agaccccgca gtactacatc
     1681 aacttcgatt ccaaccgcat cgcccgcgcc gccggactgc agcagaccgt cttctacgaa
     1741 tacatctgcg agggcaaggc atcgaaggac gccaccttct gctgcggcac aaacgtcctt
     1801 ttccgccgtg aggccttgaa aagcgtgggc ggattcgacg agtcctccgt aaccgaggac
     1861 tttgccactt cacttcaatt ccacctcaac aagtggaaga cgacgtacat caacagggtc
     1921 cgcaccttcc agataggccc ggaggacctc gggggctatt aaaaacagca gttcaggtgg
     1981 tccctgggga cgataggact cctgaggaag gtcataacga ccttcgtcct ccggcctcgg
     2041 gccctcacac tccttcagtg gtgggaatat ctcctgtcga gcacgtggta ttttatcggc
     2101 ttcgtcttct tcatcatgat gatatgcccc cctatctacc tctttttcaa tgttcccgtc
     2161 tatttcgcgc gccccgattt ctacctcctc atcttcgtcc cctacatcgt catcaccctt
     2221 accaccttct actggac
//
LOCUS       DAOPOA010000402         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_52870_length_2230_cov_2.845977, whole genome shotgun sequence.
ACCESSION   DAOPOA010000402 DAOPOA010000000
VERSION     DAOPOA010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52870_length_2230_cov_2.845977"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PKJ17_08405"
     CDS             complement(<1..356)
                     /locus_tag="PKJ17_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOD76034.1"
                     /translation="MASELVINVTYSETRIAFLENGTLVEFFIEKKNDHSMVGSIYKG
                     KVVRIVPGMDAAFIDIGLEKSAFLYVGDIILDRGMYEEFDSSDFHFEVGERIEGVLED
                     GQELIVQVSREPIGQKG"
     gene            complement(346..>2230)
                     /locus_tag="PKJ17_08410"
     CDS             complement(346..>2230)
                     /locus_tag="PKJ17_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HOD76035.1"
                     /translation="KDPIVIGGGPLMLNPKPYERFFDIIVAGEGDEILRSLLETARDM
                     KGEPRDSIIREMARLEGVYSSRVPSPQVKRLFISDLDSAYHPVRPPIPTVDSVHNRLN
                     IEISRGCGNGCRFCLAGFGYRPYRERSFEAVRAVIDEGLRNTGYEEISLLSLSSGDYP
                     FLFDVLEYARSTYRGLSVSLPSLKIGSIGKDEISAMGEMARTGFTFALEAPTASLRSR
                     LNKDIDVETLVAQLPHLRALGWRRLKLYLMVGFPWETDDDLLAIRDVITPFRAAGMDV
                     NLSVSPFTPKPHTPFQWLPMDEEGVLAEKIMLIKDALKKTGVRVRYRDTSVSVVEGIV
                     ARADERLASLFEYLHDRGVRLEAWREFFSFEPYRTWFEENSADMRAYTGSRDRAVGSP
                     WDIVDMGFDEAFFGTELDKAESGEMTVSCLAGCAACGLGCSLPQRTPRQEKPEGIVVS
                     DAASPAAEAAGAPKKFTFRYGKYGDGRYIGHLDVMNILVRAMKSAGITMRTRGKYHPL
                     PKIALTDALPVGVESFTEFIEVETGSAQQVDVSTVAMINEKLPSGIKIYEFIEGSLRD
                     MVKEYVFLLSADAPVEDGPTLWRRGKDRFFYIWRGKGVKQLWMQGSYTRIIKVESRRI
                     DGF"
BASE COUNT          449 a          702 c          619 g          460 t
ORIGIN      
        1 cccttctgcc cgataggctc gcgtgacacc tggacgatga gctcctgccc gtcctcgagg
       61 acgccctcga tcctctcccc cacctcgaag tgaaagtccg agctgtcgaa ctcctcgtac
      121 ataccgcggt cgaggatgat gtctcccaca tagaggaaag cggacttctc gagtcctatg
      181 tcgatgaacg cggcgtccat gccagggacg atcctgacga ccttgccctt gtagatgctc
      241 cccaccatgc tgtgatcgtt cttcttctcg atgaaaaatt ctacgagggt cccgttttcc
      301 aggaaagcga tccttgtttc gctatacgtt acgttgatta caagttcaga agccatcgat
      361 ccgtctcgat tccaccttta tgatcctcgt gtaactaccc tgcatccaga gttgcttcac
      421 gcccttgccg cgccaaatgt agaaaaacct gtccttcccc cgcctccaga gagtgggtcc
      481 atcttccact ggagcatcag cactgagaag gaagacgtat tcttttacca tatctcttag
      541 agaaccttca ataaattcgt atatttttat gcccgaaggc agtttttcgt tgatcatcgc
      601 caccgttgac acgtccacct gctgggcgct gcccgtttcg acctcgatga attcggtgaa
      661 gctttccaca cccaccggaa gggcgtccgt gagagctatc ttcggcaggg ggtgatactt
      721 gccccgtgtc ctcatcgtga tacccgcgga cttcatggcc cttacgagaa tgttcatcac
      781 gtcgagatgg ccgatgtagc ggccgtcgcc gtacttgccg tagcgaaagg tgaacttctt
      841 cggagcacca gcggcctcag cggccggcga ggcagcgtcc gaaacaacga tgccctcggg
      901 cttctcctga cggggcgtcc gttgcggaag agaacatcca aggccgcagg cggcgcagcc
      961 ggcgaggcac gacacggtca tctcgccaga ctccgctttg tccagttccg tcccgaagaa
     1021 ggcctcatca aaacccatgt cgacgatatc ccagggcgat ccgaccgctc tgtcgcggct
     1081 gcccgtatac gccctcatgt ccgccgaatt ctcctcgaac caggtccggt aaggttcgaa
     1141 agagaagaac tccctccacg cctcgagacg caccccccgg tcatgaagat actcgaagag
     1201 ggatgcgaga cgctcgtccg ccctcgctac gatgccctcc acgacgctca ccgaggtgtc
     1261 gcggtaccgc acccgaacgc ccgtcttctt caaggcgtcc ttgatgagca tgatcttctc
     1321 cgcgagaaca ccctcttcgt ccatggggag ccactggaaa ggcgtatgcg gtttcggcgt
     1381 aaaaggggac acggagaggt tcacgtccat gcctgccgcg cgaaacgggg ttatcacgtc
     1441 ccggattgcc aggaggtcgt cgtcggtctc ccagggaaaa ccgaccatga ggtagagctt
     1501 gagcctccgc cagcccaggg ccctgagatg cggaagctga gctacaaggg tctccacatc
     1561 gatatccttg ttgaggcggg accggagtga ggccgtcggc gcctcgaggg cgaaggtaaa
     1621 ccccgtgcgc gccatctccc ccatggcgga tatctcgtcc tttccgatgc tgcctatctt
     1681 gagcgaaggc aggctcacgg agagcccacg gtaggtgctc ctcgcgtatt ccagcacatc
     1741 gaagagaaag gggtaatccc ccgagctcag cgacagcagg gagatctcct cgtacccggt
     1801 attccggagc ccctcatcga tcacggccct gaccgcctcg aaggagcgct cgcggtaagg
     1861 ccggtagccg aaaccggcca ggcaaaacct gcagccgttg ccgcagcccc gggatatctc
     1921 gatgttgaga cggttgtgca cgctgtccac cgtgggtatc ggggggcgca ccgggtggta
     1981 cgcgctgtcg agatcgctta tgaagagacg tttcacctgc ggagaaggaa cacgggagga
     2041 ataaacccct tccagccggg ccatctcccg gatgatgctg tcccgcggct ctcccttcat
     2101 gtcgcgcgcc gtttcgagga gggaccggag tatttcatcg ccttctccgg ccacgatgat
     2161 atcgaagaaa cgttcgtacg gcttcggatt gagcatgagt ggaccgcccc cgatgacgat
     2221 ggggtccttt
//
LOCUS       DAOPOA010000403         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_52886_length_2229_cov_4.375805, whole genome shotgun sequence.
ACCESSION   DAOPOA010000403 DAOPOA010000000
VERSION     DAOPOA010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52886_length_2229_cov_4.375805"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PKJ17_08415"
     CDS             complement(<1..497)
                     /locus_tag="PKJ17_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOD76036.1"
                     /translation="MKSLLMALALVVLILSAGPAPGGAEGISPVVTTKWLAENMKDPR
                     LVVTDIRRVEEYKEEHIPGSVSLTYNAWRTMEKGISCQLPPKDDIEDNLRSAGIDRDT
                     VVVIVGKTDTDLDRAHPARVAWTMKYAGVANVAILDGGYSKWKAEGRPLAGGWTTAAP
                     SSLRCA"
     gene            complement(518..931)
                     /locus_tag="PKJ17_08420"
     CDS             complement(518..931)
                     /locus_tag="PKJ17_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOD76037.1"
                     /translation="MNEYTPIVMDHFNNPRNVGIINNHDGMGEIGDPNCGDFLRVFIK
                     VEDNIIIAIRYQIRGCPASIACASVMTELAVGKDLDEAMMIDDMDIVKALGGLPEYKL
                     HCSNLGATGLKKAILNHLARYMSSVKEQQQADKPK"
     gene            complement(928..1680)
                     /locus_tag="PKJ17_08425"
     CDS             complement(928..1680)
                     /locus_tag="PKJ17_08425"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOD76038.1"
                     /translation="MKVLEDIISSITVDAPVTDVTKGIFWTALVSRHCGLASTMVYDW
                     KMGGAKEPEETSYLQMSAASLARGALLDDTASASLGLAAINSLIDVDTVRCVDLNAGH
                     YLVENGQGKNITVIGHFPFVDELKKVAGNLWVIEKRMQPGDRPEEETATCLARSDIIA
                     ISSTTLINHTLGNILDLCPSGSTRMLLGPTTPLTSALFDHGIDIISGSVVTDPETALL
                     HIRQGANFRALKRTGSIRLVTLMKDQGGHRLQ"
     gene            complement(1752..>2229)
                     /locus_tag="PKJ17_08430"
     CDS             complement(1752..>2229)
                     /locus_tag="PKJ17_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOD76039.1"
                     /translation="VDKITAVYSSGNIDIPRPPAKEENTEGSVIPPVSGAGQGDIAAQ
                     GGTMKFAVPTNDGKLCMHFGHCEAFALIDTDAEGKVMNETYVTPPPHEPGLLPPWLAQ
                     QGVNCIIAGGMGSRAQQLFAQQGVRVVTGAQEADPKKAVENYLKGTLVTGANTCDH"
BASE COUNT          385 a          648 c          664 g          532 t
ORIGIN      
        1 gcgcagcgaa gagagctcgg cgcggctgtc gtccaaccgc cggcaagggg tcgcccctcg
       61 gccttccact tgctgtatcc gccgtcaaga atagccacat tggcgacacc ggcatatttc
      121 atcgtccagg cgacccgtgc cggatgtgcc cggtcaaggt cggtgtccgt tttgccgacg
      181 atgacgacaa cagtgtcacg gtctatccct gcggacctca ggttgtcttc gatatcgtcc
      241 ttcgggggga gctggcatga gatccctttt tccatggtcc tccaggcgtt gtatgtcaga
      301 ctgacggacc cgggtatgtg ttcttccttg tattcctcga cccttctgat gtcggtgacg
      361 acaaggcggg gatctttcat attttccgcg agccacttcg tcgtgacgac gggggatatc
      421 ccctcggcgc cgcctggggc cggtcccgcg gagaggatca aaacgaccag ggccagtgcc
      481 atgagtagag atttcatgtc tttttcctca cgttgcgcta tttgggtttg tcagcctgct
      541 gctgctcttt cacgctcgac atgtaacgcg caagatggtt caggatcgcc tttttcaacc
      601 ccgtcgcacc caggttggag caatggagct tgtattccgg cagtcctccc aaagccttga
      661 ctatgtccat atcatcgatc atcatggcct cgtcgaggtc cttgccgacg gcaagctctg
      721 tcatgacgct cgcgcaggca atggacgcgg ggcatcccct gatctgatac cgtatggcga
      781 tgatgatgtt atcttcaacc tttatgaaga ccctgaggaa gtccccgcag ttcgggtcgc
      841 cgatctctcc cattccatcg tgattgttga tgatgccgac gttgcggggg ttgttgaaat
      901 ggtccatgac gatcggggtg tattcgttca ttggagtctg tgtcctccct ggtctttcat
      961 tagagtgacg agccggatgg aacccgtccg tttcaatgcc ctgaaattcg ctccctggcg
     1021 tatgtgcagg agcgccgtct caggatcggt gactacggac ccggagatga tgtcgatgcc
     1081 atggtcgaag agcgcgctcg tcagcggcgt cgtcgggccg agcagcatcc tggtactgcc
     1141 cgaagggcaa agatcgagta tattgccgag ggtgtgattg atcagggtcg tgctggagat
     1201 ggctattatg tccgaccgcg cgaggcaggt cgcggtttcc tcctcggggc ggtctccggg
     1261 ctgcatgcgc ttttcgataa cccagagatt tcccgcaacc tttttcaact cgtcaacaaa
     1321 gggaaaatga ccgatcacgg taatgttctt cccctgtccg ttttcgacga gataatgtcc
     1381 ggcgttgaga tcgacgcatc gcacggtatc gacgtcgatc agggagttga tcgcggcaag
     1441 cccaagggac gcgctggcgg tgtcgtcaag gagcgcgccc ctggcgagcg aggcggcgct
     1501 catctgcagg taggaggtct cttccggctc cttggcgcct cccatcttcc aatcatagac
     1561 cattgtcgat gccaggccgc aatgcctgct cacgagcgcc gtccagaaga tcccctttgt
     1621 aacgtccgtg acgggagcgt caacggtgat cgaggatatg atgtcctcaa gtactttcat
     1681 tccagcgacc tctttcgggg ggagcgggtg ggcgcgacgc gccagcccgc tccccgtgat
     1741 cccggcttca cttagtggtc acatgtgttc gcgccggtga cgagcgttcc tttaaggtaa
     1801 ttctcaaccg ccttcttcgg gtctgcttcc tgtgcgcctg tcacgaccct gacgccctgc
     1861 tgggcgaaga gctgctgggc gcggcttccc atgcctccgg ctatgatgca gttcacgccc
     1921 tgctgcgcga gccacggcgg gaggaggccc ggttcgtggg gcggaggcgt cacgtatgtt
     1981 tcgttcatga ccttgccctc ggcatcggtg tcgatgagcg caaaggcctc acaatggccg
     2041 aaatgcatgc agagcttgcc atcgttcgta ggtacggcga atttcatggt gcctccctga
     2101 gcagcgatat ccccctggcc cgcgccactc acgggtggta tgacagagcc ttccgtattc
     2161 tcttcttttg ccgggggccg ggggatatcg atattgcctg atgagtagac cgcggtgatc
     2221 ttgtccacg
//
LOCUS       DAOPOA010000404         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_53107_length_2222_cov_3.602677, whole genome shotgun sequence.
ACCESSION   DAOPOA010000404 DAOPOA010000000
VERSION     DAOPOA010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53107_length_2222_cov_3.602677"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PKJ17_08435"
     CDS             <1..510
                     /locus_tag="PKJ17_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOD76040.1"
                     /translation="RYGNAILSRYPIRPVKAGALPSRRKEPRRGALWVEVDIKGQTIQ
                     VINTHLGVTPVRRRTQSATLLGPDWLGSPLCRPPVVLCGDMNATPGSYVHHSFSLRLA
                     DAQLALPGNHRPKRTWPSITPFLRLDHVFLSRDILVRNCRVPRSMVAKMASDHLPLLV
                     EAQLPDTAA"
     gene            complement(518..1951)
                     /locus_tag="PKJ17_08440"
     CDS             complement(518..1951)
                     /locus_tag="PKJ17_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOD76041.1"
                     /translation="MPSCHVVRGIILVILCCAIPYPIACTPVAGAGGRAFVPPSGPVD
                     PANSFVAVVREVGPSVVSIRAERVVEGMQLQVQGESVDEEGLRRFLRDMLRGKKRTAV
                     REASGLVMSREGHILTNHHVVADAFKITVQLFDGTVLEGTIVGADSKTDLALLRVEAA
                     GLKVPALGDSDRIEPGEWAIAIGSPYGFQRSVTTGVISGKGRAGTRLTPASDLIQTDA
                     SINPGNSGGPLVNIRGEVIGINSMVIRPGQGIGFAIPINVVKRVVGDLIAHGRVLRSW
                     VGLGLQEMTPEIRDHFGLDRPGALVRQVIEKSPSDRAGLKAGDVIVSIDGKEAANTQI
                     VMNEVLEKRIGQTVKMEVIRNGKRFSTTVTTVDEEVGVPEEPPPVDVETKRPFGLEVR
                     TITPGIAQKLERPELEGVVIIRVGMGSPASTESLMRDDVILSVDGRRIKDEKEYEAAL
                     KKVRPEKGALMMIDRWGSTFFVVLREG"
     gene            complement(1967..>2222)
                     /locus_tag="PKJ17_08445"
     CDS             complement(1967..>2222)
                     /locus_tag="PKJ17_08445"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOD76042.1"
                     /translation="EALCRARDRKGLWKRADRGDIRNLPGAGVAYERPSLPEIRVETA
                     HVSVTEAACRVVDGLEALGFIDRRRKAGKRRSLDGKVIFL"
BASE COUNT          420 a          756 c          586 g          460 t
ORIGIN      
        1 cgctacggaa acgccatact cagcagatat cccatccgac ccgtgaaggc cggcgctctc
       61 ccctcgcggc gcaaggaacc ccgccgcggc gctctctggg tcgaggtcga cataaaggga
      121 cagaccatac aggtgatcaa cacccacctg ggggttacac ccgtcaggcg gcggacgcaa
      181 tcggcgaccc ttctcggtcc cgactggctg ggcagccccc tgtgccgtcc cccggttgtc
      241 ctttgcggag acatgaacgc gacccccggg tcctacgtcc atcacagctt ttcgctcaga
      301 ctggcggatg cccagctcgc ccttccggga aaccatcgtc cgaagagaac atggccgagc
      361 atcaccccgt tcctccgcct cgaccacgtc ttcctttcac gggacatact cgtcaggaat
      421 tgcagggttc cccgctcgat ggtcgccaag atggcgtcag accatcttcc cttgctcgtg
      481 gaagcacaat tgccggacac agcggcttga caggcctcta tccttcccga agaacaacga
      541 aaaaagtcga gccccacctg tcgatcatca taagggcgcc tttttccggg cggaccttct
      601 tcagcgcggc ttcatattcc ttttcatcct ttatccgcct gccatccaca gagaggatca
      661 catcatcgcg catgagggat tcggtgctgg ccggagagcc catgcccacc cggattatga
      721 cgactccttc gagttcgggc ctctccagct tctgagcgat cccgggcgtt atcgtcctga
      781 cctcaaggcc gaagggtcgt ttcgtttcaa cgtcaaccgg cgggggctct tccggtaccc
      841 ccacctcttc atccacggtc gtcacggtgg tgctgaaacg tttcccgttt cggatgacct
      901 ccatcttcac ggtctgacct atcctctttt ccagcacctc gttcatgacg atctgtgtat
      961 ttgcggcttc cttcccatcg atgctgacga tgacatcccc cgctttcagg cctgccctgt
     1021 cagaaggact tttttctatg acctgtcgca caagggctcc cgggcggtcg agcccgaaat
     1081 gatcccgtat ctcgggagtc atctcctgaa gccccagccc cacccaggaa cgcaaaaccc
     1141 ttccgtgagc gatcaagtcc ccgacaaccc ttttgacgac attgatgggg atcgcgaaac
     1201 cgataccctg acccggccgg atgaccatgg agttgatgcc gatcacctcg cctcttatgt
     1261 tcacgagcgg gccgccactg tttccagggt tgatggacgc gtcggtctgt ataagatcgc
     1321 tggccggtgt cagtctggta ccggccctgc ctttcccgct gatcacaccg gtggtcacgc
     1381 tcctttgaaa tccatagggg gaaccgatcg ctatcgccca ctctcccggt tctatccggt
     1441 ccgaatcccc gagagcgggc accttgagac cggcagcttc tacgcgaagc agggcaagat
     1501 ccgtcttgct gtcggcgccg acgatcgttc cttcgaggac ggtgccgtcg aagagttgaa
     1561 cggttatttt gaaagcatcg gcaacgacat ggtgatttgt cagaatgtgg ccctcgcggc
     1621 tcatgacaag ccccgaggcc tcccggaccg ctgtgcgttt tttgcctctg agcatgtctc
     1681 tcaggaatcg tcgcaggccc tcctcgtcaa cgctctctcc ctggacctgg agctgcatcc
     1741 cctcaacgac tctctccgct cgtatcgaca cgaccgacgg cccgacctcc cgaacgacgg
     1801 cgacgaagga attggcggga tcgaccggac cggaaggcgg cacaaacgcc cggcctcccg
     1861 cgcccgcaac gggcgtgcac gcgatgggat aggggattgc gcagcacaaa atgacgagaa
     1921 tgatgccccg gaccacatga catgacggca ttgtcaccac tctcctctag agaaagatca
     1981 ctttcccgtc caatgaccgc ctcttaccgg ccttgcgcct cctgtctatg aagccaagcg
     2041 cttcaagccc atcaacaacc ctgcaggcgg cctcggtaac ggaaacgtga gccgtctcga
     2101 cgcggatctc cggcagggat ggcctctcgt aagcgacacc cgcgccgggg aggttcctga
     2161 tatctccgcg gtccgctctt ttccagaggc ccttccggtc tcgggccctg caaagagctt
     2221 cg
//
LOCUS       DAOPOA010000405         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_53114_length_2222_cov_3.072450, whole genome shotgun sequence.
ACCESSION   DAOPOA010000405 DAOPOA010000000
VERSION     DAOPOA010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53114_length_2222_cov_3.072450"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PKJ17_08450"
     CDS             <1..184
                     /locus_tag="PKJ17_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76043.1"
                     /translation="AVNLSFTLGICVFETVYSLDTDILRGFRNAYVFGFIACFAAMVL
                     SLLSIRVKGRTGGERG"
     gene            209..397
                     /locus_tag="PKJ17_08455"
     CDS             209..397
                     /locus_tag="PKJ17_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76044.1"
                     /translation="MGHIGPTELRLDRLPGESFLYGQLALATGGGWSVPSRGKIVVPL
                     VGPKRIAGKEQFREVMVR"
     gene            394..1053
                     /locus_tag="PKJ17_08460"
     CDS             394..1053
                     /locus_tag="PKJ17_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76045.1"
                     /translation="MRGILRDDERARVDEGVKEIERRTGAQIVVAVVERSDSYVELPW
                     KAFALGASITCLVIFALGFFAPYRASQATGFVAIAGMLAFGGLCALLTVLMPGFARLF
                     LSRYRSETEARQYAESLFLKREIFATAGRTGILLFVSLFEGQAVLLPDRGLAGSLAGD
                     AIESVVAPMAASLSRGRTGEALEKGLGRLLDILGGAELAGPGDAGENELPDEVIEERR
                     A"
     gene            1050..1988
                     /locus_tag="PKJ17_08465"
     CDS             1050..1988
                     /locus_tag="PKJ17_08465"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOD76046.1"
                     /translation="MKRTFFPAFLSVILLVLSTAMAADVPYLTGRVTDNAGILSAESR
                     KALTEKLKVYEDRTGNQVVVLTIPTLDGEGIEEYAVKVFESWKLGQKGKDNGVLMIVV
                     PEDRRMRIEVGYGLEGTLTDLMAGRIIQQVMTPRFRNGDYDGGITEGAAAVIKVLEGG
                     EAPPATGGTGSSVRSGGITEPDLPIAQRILFGAFIFGIIGLFTVIGIVTPGVGWFLYF
                     FLIPFWAMFPIVVLGVRGALICLAGYLVVFPVAKLLLKSSDFYRKGVMSLRAKGGASI
                     GGFTFSSGRSGGFSSSSSGFSGGGGSSGGGGSSGSW"
BASE COUNT          401 a          641 c          742 g          438 t
ORIGIN      
        1 cgcggtcaac ctgagcttta ccctggggat atgcgtcttc gagaccgtct attccctgga
       61 caccgacatc ctccggggct tcaggaatgc ctatgtgttc ggattcatcg cctgcttcgc
      121 cgcgatggtg ctctcgcttc tgtcgataag ggtgaaagga aggacaggtg gggagagggg
      181 ataggaccta taggagataa tgggacctat gggacatata ggccccacgg aactgcgtct
      241 ggacaggttg ccaggagaga gctttctcta cggtcaatta gcgcttgcaa caggtggtgg
      301 gtggagtgta ccctctaggg ggaagatcgt ggtgcctttg gtggggccaa agaggattgc
      361 cgggaaagaa cagtttcgtg aggtgatggt gagatgaggg gtatcctgag ggatgacgag
      421 agggcccggg tcgatgaggg cgtaaaggag atcgagaggc ggacgggggc gcagatcgtg
      481 gttgccgtcg tggagcgcag cgacagctac gtggagctgc cgtggaaggc cttcgccctt
      541 ggcgcgtcca tcacgtgcct cgtcatattt gccctggggt tcttcgcgcc ctaccgggcc
      601 tcgcaggcga cggggtttgt cgccattgcg ggaatgcttg ccttcggagg tctctgcgca
      661 ctccttaccg ttctcatgcc gggcttcgcg cggctctttc tgtcgcggta ccggtcagag
      721 acggaggcgc ggcagtacgc cgaatcgctc ttcctcaagc gcgagatctt cgcgaccgcg
      781 gggaggacgg gcatcctcct gttcgtgagc ctcttcgaag gacaggcggt ccttcttccc
      841 gaccgcggcc ttgcggggtc cctggcgggg gacgccatcg agtccgtcgt cgcgccaatg
      901 gcggcatcgc tctcccgcgg caggacaggc gaggccctcg agaagggtct cggcaggctc
      961 ctcgacatcc tcgggggggc agaattggcc ggtcccgggg atgcgggaga gaacgaactc
     1021 cccgacgagg tcatcgagga gaggcgtgca tgaaaagaac attctttccg gccttccttt
     1081 cggtgatcct cctcgttctt tcgacggcca tggccgccga cgtgccctat ctcacgggcc
     1141 gggtgaccga taacgccggg atcctctccg cagagagccg caaggcgctt acggaaaagc
     1201 tcaaggtcta cgaggaccgc accgggaacc aggtagtcgt cctcaccatc cccacccttg
     1261 acggcgaagg tatcgaagaa tacgccgtca aggtcttcga atcatggaag ctgggccaaa
     1321 agggaaagga caacggggtc ctgatgatag tcgtcccgga ggacaggcgg atgcgcatcg
     1381 aggtgggcta tgggctcgaa ggcacactga cggacctcat ggcgggcagg atcatccagc
     1441 aggtcatgac accccgcttc cgcaacgggg attatgacgg cgggatcacc gagggtgccg
     1501 cggcggtcat caaggtgctc gaaggcggcg aggccccgcc ggcaacgggc ggaaccgggt
     1561 cctccgtgag gtccgggggc atcacggagc ccgaccttcc catcgcccag cgcatcctct
     1621 tcggcgcttt tatcttcggt atcatcgggc tcttcaccgt catcggcata gtcacgcccg
     1681 gagtgggctg gttcctgtac ttctttctga tacccttctg ggccatgttc cccatcgtcg
     1741 ttctcggcgt caggggagcg ctcatctgcc ttgccggata cctcgtcgtc ttccccgtgg
     1801 cgaagcttct tctcaagagc tccgactttt acaggaaggg tgtgatgagc cttcgagcca
     1861 agggcggcgc ctccatcggc ggtttcacct tcagctccgg ccgcagcggc ggtttctcga
     1921 gcagcagctc gggtttctcc ggcggcggcg gatcctccgg cggcggcggc tcgtcgggaa
     1981 gctggtaagc cggtaagccg ggaaacgttc tgcggacaac ggcgaaaaag ggaccggcag
     2041 gaacggggga actttttcag agaccctcgt gtccaacggg agagaaagcg aagacgcgat
     2101 gatatccaca gccgaaagga gaatgaaatg aaacggtcgt tattggcgtt ccttggcagc
     2161 ctcttgcttg ccgtcctgtc gatgacccct gccgcggcat cacctgcggg tcccttcagt
     2221 gc
//
LOCUS       DAOPOA010000406         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_53216_length_2219_cov_3.089649, whole genome shotgun sequence.
ACCESSION   DAOPOA010000406 DAOPOA010000000
VERSION     DAOPOA010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53216_length_2219_cov_3.089649"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1924)
                     /locus_tag="PKJ17_08470"
     CDS             complement(<1..1924)
                     /locus_tag="PKJ17_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOD76047.1"
                     /translation="MRSSGFVPCLSLRLIFLAIAVLVTTLLPASLSAQTNPSEFAEMI
                     GRERRVVESTRRDIREYISKQPERMARISERTQQLNQEVFRLFITYNLEENNPIEMRD
                     MLRQITMVRDQAVSLIAPVKEEIESIQGLKKVIRTHVNDYERLASDNSITGVHRSATQ
                     YVAELKEAVGLLESAETVVEIIPNAAEQLIARLEPRRTVIEDDLRLAWKKYYLSPPDA
                     QNFFTTDAWRKVGMLLRSWAGFSAYWLIPYSQNSAAITSVLAKAAIFSFGIFGAFLVI
                     FTRLRRKCPALPAVRYFLPCCCYTALGLPLMVTGATTDIGPLSTSTFVAEVMLAAGLV
                     SLGWNLRRLSADDEAAYKHNPLWSFWVVFAAGVLVQLFHLPMLAYSPLLAFLFIVAGI
                     YSHTLERRDQHILDRRLLAITAWLSYTLAATTLFGWGSLSLLAATVWFAVMLNVELGA
                     GLTACLRKIRTVTNYGRTAAGRLVEGVVFPMVFLGLFAVTIFWVILYIGGMPLVTRII
                     AWHMVVGYLSLNPSMVVVIIAVLFITRSLVVLVNALVTFVATRLGAGTGEGVLKSLHA
                     ISTYVIWSLYILLSLKLIGVSVGHLAIIAGGLSIGAGLGLQDMIRNFFSGLVLLFGRS
                     LHPGDEIQLGDVRGTVM"
BASE COUNT          511 a          630 c          650 g          428 t
ORIGIN      
        1 tcatcaccgt cccccgtaca tcgccaagct ggatctcgtc gccgggatgg agcgacctgc
       61 cgaaaaggag cacgaggccg ctgaagaagt tcctgatcat gtcctggagc cccaggccgg
      121 ctcctatgga caatccgccg gcgatgatag ccaggtggcc gacgcttacc cctatcagct
      181 tcaacgagag gagaatgtac aatgaccata tcacgtaggt ggatatggca tgaagcgact
      241 tgagaacgcc ttctcctgtc cccgccccaa ggcgggtagc cacgaacgtg acgagggcat
      301 tgacgagaac gacaagggac cgcgtgatga aaagaacggc tatgataacg acaaccatgg
      361 aaggattcag cgagagataa ccgacaacca tgtgccacgc gatgatcctg gtgacaagag
      421 gcatgccgcc tatatagaga atcacccaga aaatggtgac agcgaagagg ccgaggaaga
      481 ccatcgggaa gacaacgcct tcaacaagcc tcccggcggc cgttcgcccg tagtttgtca
      541 ctgtcctgat cttgcgcagg cacgccgtca atccggcccc cagttccacg ttcagcatga
      601 ccgcgaacca caccgtggcc gcgaggaggg agaggctgcc ccagccgaag agggtcgtgg
      661 cggcgagtgt gtacgacagc caggctgtta tggcaagcag tctcctgtcc aggatatgct
      721 gatcccttct ctcaagtgtg tgtgagtaga tgcccgccac tatgaagagg aaagcgagca
      781 ggggagagta ggcaagcatc gggagatgga agagctgcac gagcacaccc gctgcgaata
      841 ccacccagaa agaccagagg gggttgtgct tgtatgccgc ctcatcgtcc gcggagaggc
      901 gcctcaagtt ccagcccaga ctgaccaatc cggccgccag catgacctcc gccacaaacg
      961 tcgacgtact taaaggaccg atgtccgtcg tggcgcctgt gaccatgaga ggaaggccca
     1021 aagccgtgta gcagcagcac ggcaggaagt accgcaccgc gggcaacgcg ggacactttc
     1081 ttctcagacg ggtaaagatg accagaaagg cgccgaatat gccgaaggag aatatcgccg
     1141 cttttgccag gacggatgtg atcgcggcgc tgttctggct gtatgggatc agccagtagg
     1201 ccgagaaacc tgcccagctc ctgagcagca tgccgacctt tctccacgcg tcggtcgtga
     1261 agaagttctg ggcgtcgggg ggtgaaagat agtacttttt ccacgcgagc ctgaggtcat
     1321 cctcgatcac ggtccttctc ggttcgagcc gcgcgatgag ctgttcggcg gcattgggga
     1381 tgatctcgac aacggtctcg gcggattcga gaagccccac cgcctccttc aattcggcga
     1441 cgtactgcgt ggcggaccgg tgcaccccgg tgatggagtt gtccgacgcg agacgctcat
     1501 aatcattcac atgcgtgcgt atgacctttt tcaggccttg gatggactcg atctcctctt
     1561 tcacgggagc tatgagcgag acggcctgat cgcgcaccat cgtgatctgc ctgagcatgt
     1621 cccgcatctc gatggggttg ttctcctcca gattgtacgt tatgaaaagc cggaagacct
     1681 cctgattgag ctgctgcgtc cgttcactta tcctggccat gcgctccggc tgtttggata
     1741 tgtactcgcg gatgtcccga cgggtcgatt cgacgaccct cctctcccgg cctatcatct
     1801 ccgcgaattc ggatgggttg gtctgcgcag acaggctggc gggcagaagc gtcgtcacga
     1861 gaaccgcgat ggccaagaat atcagacgca gggaaagaca gggaacgaag ccgcttgatc
     1921 tcattggacg ccggtatcca tacaatggca taacgttacg cccttttcaa gccaaataaa
     1981 aggacaggct tgaaccgctt gacagcctgt cgataaagtc tttgccgagt caagtttatg
     2041 tttttggtag agtttgtgtt ccgtcgtcat cccggcgaag gccgggaccc aggaaaaccc
     2101 cctgctgcag cagaaaacac acaaaggatg gtgccgaaca acgcaatacg ggagagtcgt
     2161 tgaagatttc actgttcgct agtcaagtac tgccagtccc ttctggatcc cggccttcg
//
LOCUS       DAOPOA010000407         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_53291_length_2216_cov_3.405831, whole genome shotgun sequence.
ACCESSION   DAOPOA010000407 DAOPOA010000000
VERSION     DAOPOA010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53291_length_2216_cov_3.405831"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..310)
                     /locus_tag="PKJ17_08475"
     CDS             complement(107..310)
                     /locus_tag="PKJ17_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002856364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HOD76048.1"
                     /translation="MPYVNIKVTKEGVTAEQKEKLIRGVTQLLFDVLGKKPQTTYVVI
                     DEVETDNWGVGGETVTVRRKRVS"
     gene            complement(367..936)
                     /locus_tag="PKJ17_08480"
     CDS             complement(367..936)
                     /locus_tag="PKJ17_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76049.1"
                     /translation="MCRICDVGLVEDPASVGDEGDVYFRDHHDVDPSFPAGHVFLVEV
                     GGEEEFEAVRSVLIDAGIPFVVQGTTAAPHDAGVSGVFAVHTPARIAVPREYMGQARD
                     VLEARIFRPVSELRVKALETRLFSDIIWSAVWFAAGFGIACLVVPGDWDQGLRFLVYA
                     VAGLVAVPVGNAALRPGRGAKEKHPWLNE"
     gene            complement(1058..1975)
                     /locus_tag="PKJ17_08485"
     CDS             complement(1058..1975)
                     /locus_tag="PKJ17_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD76050.1"
                     /translation="MQRSFSICSRVLLTLLAVFLLQGQASAADKLTRIAENVYAYVDT
                     KGASPKNSFGANAGIIIGRDGIVVVDTLISAREAKRFIRDIRAVSKKPIRYVINTHYH
                     LDHVFGNSEFVKLGAVIVAEENDRREMERSAAETLKNIKGYGLTREDMKGTKPAYPTV
                     TYSERMTIDIGGERVELIHARHSHTGGDTLVYLRDKKVLFAGDILFTDYHPFIAEGNI
                     EEWVKELDEIASMDVEKIIPGHGPVSGKKDVQAMKEYIIAFDRMAKELAAKYGDPAKI
                     AAELKSGLPERRESAFLIGANVEMKYLKK"
BASE COUNT          414 a          723 c          575 g          504 t
ORIGIN      
        1 cggggtttcg ggggacctct acagcttcag actggggatc acggccctgg gcgtgctcac
       61 cctgctcgcg agcacgttcg tgttccgcct cagggaactg aaatgatcag ctcacgcgtt
      121 ttctccttac cgtgaccgtt tcaccgccca ctccccagtt gtccgtctcc acctcgtcga
      181 tgacaacgta ggtcgtctgc ggcttctttc ccaggacatc gaagaggagc tgcgtcacgc
      241 ccctgatcag cttctccttc tgctcggcgg tgaccccttc tttcgttacc ttgatgttca
      301 cgtacggcat cgttgccctc ccctatccat gtgaccgtcc ggcagacggg gctttaagcc
      361 gcccggtcac tcgttcaacc aagggtgttt ctctttcgcg ccccgccccg gcctcaacgc
      421 cgcgttccca acaggtaccg cgacgaggcc cgcgacggca tacaccagga accgcaggcc
      481 ctgatcccag tcaccgggaa cgacgaggca ggcaatgccg aacccagccg caaaccacac
      541 cgccgaccat atgatatcgg agaagagcct cgtttccagg gccttcaccc tcagttccga
      601 taccggccgg aagatacgcg cctcaaggac atctctcgcc tgccccatgt attccctcgg
      661 caccgctatt ctggcaggcg tatgtaccgc gaaaacaccg gaaaccccgg catcatgtgg
      721 cgcggccgtt gtcccctgca cgacgaaggg gatccccgca tcgatgagca ccgaccggac
      781 ggcctcgaat tcttcctctc cgccaacctc gacaaggaag acgtggcccg cggggaacga
      841 cgggtccaca tcgtgatgat cgcgaaaata gacatcacct tcgtcaccaa cggatgccgg
      901 gtcctcgaca agcccgacat cacagatccg gcacactgtt atcccttcac catatctcgt
      961 acgacaacgc gggcaaagaa tgactcatct cctcctggaa cgccttgaat gtttgcctcc
     1021 gcccctcaag ctattcaagc gactcaagcg actcaagcta tttcttcaaa tacttcatct
     1081 ccacgttcgc cccgatgagg aaggcgcttt cgcggcgctc gggcaggccg ctcttcagtt
     1141 ccgccgcgat cttcgcagga tcgccgtact tcgccgcgag ctcctttgcc atcctgtcga
     1201 aggcgatgat gtactccttc atggcctgga cgtccttttt gcccgacaca gggccatgcc
     1261 ccgggatgat cttctcgaca tccatcgacg cgatctcatc gagctctttc acccattcct
     1321 cgatgtttcc ctcggcgatg aacgggtggt aatccgtaaa gagaatgtcg ccggcaaaga
     1381 ggaccttttt gtcgcggagg tagacgaggg tgtctccccc ggtgtgggag tggcgtgcgt
     1441 ggatgagttc cacccgctca ccgcctatat cgatggtcat cctctccgaa taggtcaccg
     1501 tggggtaggc gggcttcgtg cccttcatgt cctcccgggt gagaccgtat cctttgatgt
     1561 tcttcagcgt ttcggcggcg gacctttcca tctccctcct gtcgttctcc tcagcgacga
     1621 tcacggcccc gagcttgacg aactccgaat tgccgaagac atggtccagg tgataatggg
     1681 tattgatgac atacctgatg ggctttttcg acacggcccg gatgtccctt atgaagcgct
     1741 tcgcctctct ggcgcttatg agggtgtcca cgacgacaat gccgtccctg cctatgatga
     1801 tccccgcgtt ggccccgaag ctgttcttcg gcgacgcccc cttcgtatcc acataggcgt
     1861 acacgttctc ggcgatgcgt gttaatttgt cggccgcaga tgcctgtccc tggagcagaa
     1921 acaccgcgag aagcgtcaat aacactcttg agcagatgga aaaagacctt tgcatgcttg
     1981 tcctccctga tcgttccggc gctgtctccc cgcggtgccg tgccttgacc ggtaagctta
     2041 tagtagcatg atgctcgccg gattaaaaca gaatgagggc ccagggaagt gttgacattt
     2101 ccctccattc atgtaaattc attgtcattc gcggaggtgc acatgctcac agggaagtac
     2161 tctcttcttg ctttctgtct cgtcggcctg cttctcgggg gcggctgtga aacggt
//
LOCUS       DAOPOA010000408         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_53386_length_2213_cov_4.172845, whole genome shotgun sequence.
ACCESSION   DAOPOA010000408 DAOPOA010000000
VERSION     DAOPOA010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53386_length_2213_cov_4.172845"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="PKJ17_08490"
     CDS             complement(<1..1421)
                     /locus_tag="PKJ17_08490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOD76051.1"
                     /translation="MDDQALQRDEMQEIIDEFIAESSELMDNVIQDIVVIEQSQDEEV
                     VNSIFRAVHTIKGTSSFLGFNALSQLAHKAEDVLGVIRKGEMATDQTVADILLESFDL
                     MKLMIEDIRDQGSENQDASAVIVKLTDLLDPAKKAAEPQPEETKKIGEILVEEKIISE
                     SELEDVLRKQETEKDKKVGEIAVEEKYITETQLNKALVKQKAPKMEEQSIRIDVKKLD
                     ELMNLVGELVLGKNRLILVNSMARKGEESEAVFDNLTDITNYIEVITNELQLSVMRAR
                     LVPVSKVFNKIPRMVRDLCSEFKKDIELKVEGEETELDRSLIEALTDPLIHIIRNSVD
                     HGIELPEERAAKGKRAKGLLSIRAYNEGNHVIIEVYDDGKGINIQAVKDKVKEKGLMN
                     EAELASLSPKEAMNLIFIPGLSTAQKIRKVSGRGVGMDVVRTNIEKMNGQAHIDSEEG
                     KWTKLTIKLPLTLAIMRALIVKVA"
     gene            complement(1414..1797)
                     /locus_tag="PKJ17_08495"
     CDS             complement(1414..1797)
                     /locus_tag="PKJ17_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD76052.1"
                     /translation="MKTILIVDDSATIRKLLAYILKRKNYIIAEAEDGMDAMEKLSHV
                     QVDLVIVDLNMPNMDGIEFVKNLRDNYYYMDTPVIMLTTTKDDKLKKDALDAGVNMFL
                     NKPVQPNFLLYKVESLLQGEEEYNG"
     gene            complement(1801..>2213)
                     /locus_tag="PKJ17_08500"
     CDS             complement(1801..>2213)
                     /locus_tag="PKJ17_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOD76053.1"
                     /translation="SIIATDISSKVLESARKAVFSQYSVRNTDEAIKRKYFTEENNMY
                     KLKESVKKNVKIDFMNLMDDDAYRLYRQMDFIFCRNVLIYFDEKMKKKVVDHMYECLK
                     PKGFLTIGHAESLHNISRAFKPLVFPGTIAYQKG"
BASE COUNT          396 a          636 c          563 g          618 t
ORIGIN      
        1 gccactttca cgataagggc ccgcatgatg gcaagggtca gggggagctt gatggtgagc
       61 ttcgtccact tgccttcctc ggaatcgatg tgcgcctgcc cgttcatctt ctcgatgttc
      121 gtcctgacga catccattcc aacgccgcgg ccggagacct tccttatctt ctgagccgtg
      181 ctcagacccg ggatgaagat gaggttcatc gcctctttgg gagacaggct tgccagctcc
      241 gcctcgttca tgagcccctt ctccttcacc ttgtccttca ccgcctggat gttgatgccc
      301 ttcccgtcgt cgtaaacctc gatgatgacg tgattgccct cgttgtaagc ccttatggat
      361 agaagaccct tggcccgctt tcccttcgcc gcgcgctctt ctggcagttc gatgccgtgg
      421 tcgacggaat tccgtatgat gtggatgaga gggtccgtca gcgcttcaat gagagaccgg
      481 tcaagctccg tctcctcgcc ttcgaccttg agctcgatgt ccttcttgaa ctccgagcaa
      541 aggtccctca ccatcctggg gatcttgttg aagaccttgc tgacgggcac cagtctcgcc
      601 ctcatgactg aaagctggag ctcgttggtg atgacctcta tgtagttcgt tatatccgtg
      661 agattgtcga agacggcctc gctctcctca cccttcctcg ccatgctgtt gacgaggatg
      721 agcctgtttt tcccgaggac gagctcaccg acgaggttca tcagctcgtc gagcttcttg
      781 acgtcgatcc tgatcgactg ctcctccatc ttgggggcct tctgtttcac cagtgccttg
      841 ttgagctggg tctccgtgat atacttttcc tcgacggcga tctcgccgac cttcttgtct
      901 ttttccgtct cctgtttcct gaggacatcc tcaagctcgg attccgagat gatcttctcc
      961 tccacgagga tctcgccgat cttcttcgtc tcctcgggct gaggctcggc ggccttcttc
     1021 gcgggatcga gaaggtccgt gagcttaacg atgacggcgg acgcgtcctg gttctcgctt
     1081 ccctggtccc ggatatcctc tatcatcaac ttcatgaggt cgaaggactc gagaaggatg
     1141 tcggcgacgg tctggtccgt ggccatctcc cccttgcgga tgaccccgag gacgtcctcc
     1201 gccttgtggg cgagctggga cagggcgttg aaccccagaa agctcgacgt tcccttgatg
     1261 gtgtgaacgg cccggaaaat gctgttcacg acctcctcgt cctggctctg ctcgatgacg
     1321 acaatgtcct gaatgacatt gtccatgagt tcactcgact ccgctatgaa ctcatcgatg
     1381 atttcctgca tctcatcccg ctggagagct tgatcatcca ttgtactcct cctccccctg
     1441 caaaaggctt tctaccttgt acagtaagaa atttggttgc accggcttgt tgaggaacat
     1501 gttgacgccg gcgtcaagag cgtccttttt gagcttgtca tccttcgtcg tcgtgagcat
     1561 gatgacaggc gtgtccatgt aatagtaatt gtcccgcagg ttcttcacga actcgatgcc
     1621 gtccatgttg ggcatgttga ggtccacgat aacgaggtcc acctggacgt ggctcagttt
     1681 ttccatggcg tccattccgt cctcggcctc tgcgatgata taattcttcc gtttcaggat
     1741 gtatgccagg agcttcctga tcgtcgccga gtcatccacg atgagtatcg tcttcatata
     1801 tcatcctttc tgatatgcga tggtgcccgg gaataccagc ggcttgaagg cccgggatat
     1861 gttatggagc gattccgcgt gaccgatggt aaggaatccc ttaggcttca ggcattcgta
     1921 catgtggtcg acaaccttct tcttcatctt ttcgtcaaaa tagatgagga cattcctgca
     1981 gaagatgaaa tccatctgcc ggtacagtct gtaagcatcg tcatccatga ggttcatgaa
     2041 gtcgatctta acgttcttct ttacgctttc tttcagctta tacatattgt tctcttctgt
     2101 aaaatacttt ctcttgatgg cctcatcggt gttgcggacg ctgtactggc tgaagacggc
     2161 cttccgcgcg gactcgagga ccttgctcga aatatccgtg gcgatgatgc tga
//
LOCUS       DAOPOA010000409         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_54101_length_2191_cov_3.068820, whole genome shotgun sequence.
ACCESSION   DAOPOA010000409 DAOPOA010000000
VERSION     DAOPOA010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54101_length_2191_cov_3.068820"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2098)
                     /locus_tag="PKJ17_08505"
     CDS             complement(<1..2098)
                     /locus_tag="PKJ17_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOD76054.1"
                     /translation="MATVTEKPPRPYKEYRDFIGNSGLTHKDSQERIAAAIRDGIASG
                     RPVIAEGGTGNGKTVSYLVPAIEALHRNPDMKLVVSTWSIALQNQLIHGQGPGEKAEI
                     PRVLDAMKKKYGKDFKIGMNDVTVIVGAQQHLCEESEAFPREDSKETPAKSLLIESVG
                     QENYTKLNEAVKKARQDKRPILLRELDNRGIKNIPLSVREEISCRGSSGCKHFHNADK
                     TCSFATKMDHAFKNARIIVTNHAQLTMNRGLQNALLKGNVVVIDEANRLPDILTRMAN
                     ASAQLSNIRRSCDLFLESAGQMTLLTDISEKMTAAGNFADKLRAAIDATEKAVVKAAE
                     GKKSTSVALRTDDGEIVEDINFYKYLNEVFPGSDARDALENGRKAFESLVDAIKKDMA
                     PADGTRTTGKTSSSKGAFMDLIRVSERIIRGVGDLHGFGRRILKETWLTEDEKKGRRY
                     SKKARNGVMYDSIKNDNIIWFEKGTRSISMFAAPLDHIRVQRDAARALFDHSGNRPVF
                     ISATLSTDAREPNFIDFERSLGIQYWSQLNKPPIEIQEASDHHYDKQAILVIPNDLPQ
                     PEFRKDSPEGQSEESKNYFDAIAQDIIRNVKIVDGNSMVLCSSAWQASALSEQLEAAL
                     DPEKFQVLNQVESSSTEGLIRRMRQGVEPKKVLVGTDTLWNGIDLRGEALRGLFIPKA
                     PFAVPDHPVERERARRW"
BASE COUNT          360 a          629 c          640 g          562 t
ORIGIN      
        1 tccaccgcct ggcgcgctcc ctctccacgg gatggtcggg caccgcgaaa ggggccttcg
       61 gtatgaacag acccctcagg gcttccccgc ggaggtcaat accgttccag agtgtatccg
      121 tgccaaccag gaccttcttt ggttcgaccc cctggcgcat tctcctgatc agaccttccg
      181 tcgaagagga ctccacctgg ttgaggacct ggaatttctc cggatcaagc gcggcctcca
      241 gctgttcgct gagggccgag gcctgccagg cggaactgca cagaaccatc gagttcccgt
      301 cgactatctt cacgttgcgg ataatgtcct gcgcgatcgc gtcgaaatag ttctttgatt
      361 cctcggactg gccctcgggg ctgtccttcc tgaactcagg ctgtgggagg tcgttgggta
      421 tcacaaggat cgcctgtttg tcatagtggt ggtcacttgc ttcctggatc tcgatcggcg
      481 gcttgttcag ttggctccag tactgaatgc ccagggatct ctcgaaatcg atgaagttgg
      541 gctcgcgggc atccgtcgag agggtcgccg atatgaagac gggccggttg cccgagtgat
      601 cgaacagagc ccttgccgcg tcgcgctgga cccttatatg atcgaggggg gccgcgaaca
      661 ttgagatgga cctggtgcct ttctcgaacc agataatgtt gtcgttcttg atcgaatcat
      721 acatgacccc gtttctggcc ttcttggagt agcgcctccc cttcttctca tcttcagtga
      781 gccatgtttc cttcaggatg cggcggccga agccgtggag gtcgcccaca ccccggatga
      841 tcctttcgga cactctgatg aggtccatga aagcgccctt cgaagacgat gttttgcccg
      901 tggtcctggt cccgtccgca ggagccatgt cctttttgat ggcgtccacc aggctttcaa
      961 atgcctttct gccgttctcg agggcatccc tggcgtcgct cccggggaat acctcgttga
     1021 gatacttgta gaagtttatg tcctctacga tctcgccgtc atccgtcctc agcgcgacag
     1081 acgtcgactt tttcccctcg gcggccttca cgacagcctt ttcggttgca tcgatcgcgg
     1141 cgcggagttt gtcggcaaag ttgcctgcgg ccgtcatctt ctccgagatg tcggtgagga
     1201 gggtcatctg ccccgccgat tccaggaaga gatcacagct ccttcgtata ttgctcagct
     1261 gtgcggaagc gttggccatc ctcgtcagaa tatcaggcaa cctgttggcc tcgtcgatga
     1321 caacaacgtt acctttaaga agagcgttct gcaaccctct gttcatcgtg agctgggcat
     1381 ggttggtgac gatgatgcgg gcgtttttga aagcgtggtc catcttcgtc gcgaaggaac
     1441 aggtcttgtc cgcattgtgg aagtgtttgc atccgctcga gccgcggcag gagatttctt
     1501 ctctgacaga cagggggatg ttcttaatac ccctgttgtc gagctcgcgg agaaggatcg
     1561 gccgcttgtc ctggcgggcc ttcttcacgg cctcattaag cttcgtgtag ttctcctgcc
     1621 ccacggattc gatcagcagg gacttggccg gggtttcctt cgagtcttcc ctcgggaagg
     1681 cctcgctctc ctcgcacagg tgctgctggg cgccgacgat gaccgtgaca tcattcatcc
     1741 ctatcttgaa atccttgccg tatttctttt tcatcgcgtc gaggacgcgg gggatctccg
     1801 ctttctcccc ggggccctgg ccgtggatga gctggttctg cagggcgatg gaccaggtgc
     1861 tgacgacgag cttcatgtcg ggattcctgt gcagcgcctc gatcgcgggg acgaggtagc
     1921 tgacggtttt gccgttgccg gtaccgcctt cggcgatgac aggccggcct gaggcgatgc
     1981 cgtcccggat ggcggcggcg atacgttcct gggaatcctt gtgcgtgagc cctgaattgc
     2041 ctatgaagtc tctgtattcc ttgtacggtc tcggtggttt ttctgttacg gtcgccatga
     2101 aggtctcctt ctctgtgctc ccctttgggt gagcctgggt gtcttttgat aatgcgtcgt
     2161 taagattaaa gtagcgctta ccggaagagc g
//
LOCUS       DAOPOA010000410         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_54128_length_2190_cov_4.377518, whole genome shotgun sequence.
ACCESSION   DAOPOA010000410 DAOPOA010000000
VERSION     DAOPOA010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54128_length_2190_cov_4.377518"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1613
                     /gene="atpD"
                     /locus_tag="PKJ17_08510"
     CDS             192..1613
                     /gene="atpD"
                     /locus_tag="PKJ17_08510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOD76055.1"
                     /translation="MSSVEVRGKIVQVIGTVVDFRFPPDQLPPINGAIYVTNPAISDK
                     AENLILEVAQHVGDNTVRCIAMNTTDGLMRGQDAVYKGGQISIPVGKEILGRVLNVVG
                     DPVDNQGDVKTAMTYPIHRPAPSLTQQNTKIELLETGVKVIDLLEPYPKGGKVGLFGG
                     AGVGKTVVIMEMIHNIAQHHGGISVFGGVGERTREGNDLWLEMKGSGVLDKCALIYGQ
                     MTEVPGARARIGLTALTAAEYFRDEEGQDVLLFIDNIFRFTQANSEVSAQLGRMPSAV
                     GYQPTLATDLGELEERITSTLKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSR
                     QISELGIYPAVDPLDSTSRILDPQIVGEEHYQVARNVQAVLQKYKELQDIIAILGMDE
                     LSEDDKLTVARARKIQRFLSQPFFVAEVFTGQPGRYVALKDTIRGFKEIVEGKHDELP
                     EQAFFMVGTIEEAVEKAKQFMGE"
     gene            1623..2024
                     /locus_tag="PKJ17_08515"
     CDS             1623..2024
                     /locus_tag="PKJ17_08515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOD76056.1"
                     /translation="MLNLEIITPERTVVKDEVDVVEATGAEGEFGIMPGHTQFLTTLR
                     IGEIRYKKGSETTYIASSGGFAEVVDDKVTLLVDTAESAKEIDVERAKKAMERAETML
                     KTMAYDHTEYRLMEMALLRAIARIGVASKKI"
BASE COUNT          524 a          581 c          644 g          441 t
ORIGIN      
        1 gaatcccaga cgtcggagca cgcggcccgg atgagcgcga tggagaacgc gacgaataac
       61 tgcggtgaga tggtgcggta tctcaccctc gtttacaaca agagaagaca ggaaggtata
      121 accaacgaaa tgttggatat cgttggtggc gctgaagcct tgcgaggaac ataatctaag
      181 gaggaagata gatgagcagc gtggaggtca gaggaaagat tgtacaggtc attggaacgg
      241 tcgtagattt caggtttcca cctgatcagc ttccaccaat aaacggcgcg atttacgtaa
      301 ccaaccccgc tatcagcgac aaggcggaga acctcatcct tgaggttgcc cagcatgtcg
      361 gtgacaatac ggtcagatgc atcgccatga acacgactga cggtctcatg cgcggccagg
      421 atgccgtcta caagggcggg cagatatcga tccccgtcgg gaaggagatc ctgggcaggg
      481 tgctcaatgt cgtcggcgat cccgtggaca accagggaga tgtcaagacg gccatgacgt
      541 acccgatcca ccgcccggca ccctcgctga cccagcagaa tacgaagatc gagcttctcg
      601 aaacgggcgt caaggtcatc gaccttctcg agccttatcc gaagggcggc aaggtcggtc
      661 tctttggcgg cgccggtgtc ggcaagacgg tcgttatcat ggagatgatc cacaacatcg
      721 ctcagcacca cggcggcata tccgttttcg gtggtgtcgg tgagcgcacc agggagggaa
      781 acgacctctg gctcgagatg aagggctccg gtgtactcga caagtgcgcg ctcatttacg
      841 gacagatgac ggaagtcccc ggtgcccggg cgaggatcgg tcttacggcc ctcactgccg
      901 ccgagtattt ccgcgatgag gaaggccagg acgtgctcct cttcatcgac aacatcttcc
      961 gtttcaccca ggccaattcc gaggtctcgg cgcagctcgg ccgtatgccc tccgccgtcg
     1021 gttaccagcc gaccctggcg acagaccttg gagagcttga ggagcgcatc acctccactc
     1081 tgaaaggttc catcacctcc gttcaggcca tttacgtccc tgccgatgac ctcacagacc
     1141 cggcgccggc aacgacgttc gcgcatctcg acgccacaac ggttctttcc cgtcagatct
     1201 cggagcttgg tatatacccg gctgttgacc ctctggactc cacgagccgt atcctcgacc
     1261 ctcagatcgt tggggaagag cattaccagg tagcccggaa cgttcaggcg gtccttcaga
     1321 aatataagga acttcaggac atcatcgcca tcctcggtat ggacgagctc tcggaagacg
     1381 acaaactgac cgttgccagg gccaggaaga tccagaggtt cctctctcag cccttcttcg
     1441 ttgcggaggt cttcacgggc caacccggcc ggtacgttgc tctgaaggat acgatcaggg
     1501 gattcaaaga gatcgttgaa ggcaaacacg acgagcttcc cgagcaggca ttcttcatgg
     1561 tgggcaccat agaagaggct gttgaaaaag ccaaacagtt catgggagaa taagagaacg
     1621 ttatgctcaa ccttgagata attacccctg agagaacggt tgtcaaggac gaagtcgatg
     1681 tggtggaggc cacgggagca gagggcgagt tcggtatcat gccgggccac acccagttcc
     1741 tcacgaccct tcgtatagga gagatccggt acaagaaggg ttcggagacc acctatatag
     1801 catcgagcgg tgggttcgcc gaggtggtcg acgacaaggt tacattgctt gttgatactg
     1861 cggagtccgc gaaggagata gatgttgagc gggcaaagaa agcaatggaa cgtgctgaga
     1921 ccatgcttaa gacgatggct tatgatcata cggaataccg gctgatggaa atggcactgc
     1981 tccgggcgat tgcccgtata ggcgtggcgt ccaagaaaat ataatggtct tcaccataga
     2041 cgaggggcta ttcagccttt ttcctgcatt gcaggtcggt gtggttgttt gcgagattga
     2101 caacataagg tacggcgaag atggactcga ggaggtcgtt gacgatctga gagtccattt
     2161 ttcttttgaa cgtccgcagg accatccgaa
//
LOCUS       DAOPOA010000411         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_54131_length_2190_cov_3.835597, whole genome shotgun sequence.
ACCESSION   DAOPOA010000411 DAOPOA010000000
VERSION     DAOPOA010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54131_length_2190_cov_3.835597"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..442
                     /locus_tag="PKJ17_08520"
     CDS             185..442
                     /locus_tag="PKJ17_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76057.1"
                     /translation="MLEDPGTNIAPVESGSVDMLDRILVREIISVLMGSTFYLGLTVP
                     ERLDMVKHLLDLMSPKGGSRFQVPGSRFQVPGNGSRTPGKR"
     gene            complement(642..1370)
                     /locus_tag="PKJ17_08525"
     CDS             complement(642..1370)
                     /locus_tag="PKJ17_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD76058.1"
                     /translation="MNTETYGSGTPVVFIHGAGGSALSWLAQKTYLQRSNRVILVDLP
                     GHGRSDGPSSDSIDEYAQAVGDALEAIACGPAHIAGHSMGGAVAMRLSMSRPSLVKGL
                     VLIGTGAKLKVYPEILEGILRDKAATARTILDTAFSDSVPTSLKDKVFAEYMKNDALT
                     IFNDFTACNAFDLMGRLDAITVPTLVICGMADRFTPPKYSRYLAGTIAGASLELIADA
                     GHMVMIEKPAEVNAAIDRFVNSRE"
     gene            1529..>2190
                     /locus_tag="PKJ17_08530"
     CDS             1529..>2190
                     /locus_tag="PKJ17_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOD76059.1"
                     /translation="MQKNDPGPKPAAGRGRKAKTPGTGRTGQEAREQRPSVLATPGET
                     GTPDVYDFHDLFRAFMDNGPAIAWLKDEEGRHVYLNRALQHRFYDGSRDWYGKTDFEL
                     WPPEMAGRFRDRDLAVLGAEGTIDVVEEAANPDGSRSWWWNFRFPFEDSSGKRYVGGM
                     GIEISERKRLEAELSKLAAVVRHSSEFVSLATLDRQIIYVNETGAKMVGSSPEKMIGM
                     SAL"
BASE COUNT          482 a          560 c          666 g          482 t
ORIGIN      
        1 cgctatgggg tgcactatgt ttttgcttgc tgacacaaaa tacttgacgt tacccccctt
       61 cagaatcctg cggaagatct tttgccttgc ctgtgacgct gccctctccg gtcaccggga
      121 cgggcagccg ggatccgttg gcatacggca cagatggtag tgggggcttc caagggacgg
      181 gtccatgctt gaagatcctg gcacgaatat tgctcctgta gaatcgggga gcgtggatat
      241 gctggacagg atactcgtca gggagatcat aagtgttttg atgggaagca ccttctatct
      301 cggtcttaca gtgccggaga ggcttgacat ggtgaaacat ctccttgacc tcatgtctcc
      361 gaaaggtggt tccaggttcc aggttccagg ttccaggttc caggttccag gcaacgggtc
      421 ccgaaccccg ggtaagcgtt aaatcccttt ttcaaagtcc catttttttg tacaggtgtt
      481 ccactttgta agacggcaca ataaaaaagt tcaaaagttc aagagttcaa gggaaggata
      541 ctgaaaggcg gtccaccgtc aacgcaattc gcaaagattg tcgaacgggg tatcagagga
      601 gttctgtttt tctactcttg aaccttcttt atccctgaac cttactcccg cgagttcacg
      661 aatctgtcga tggccgcgtt cacctcggcc ggtttctcga tcatgaccat gtggcctgcg
      721 tcggcgatga gttcaagact tgctcctgct atggtcccgg cgagataacg cgagtatttg
      781 gggggcgtga agcggtcggc catgccgcaa atgacaaggg tcgggaccgt gatcgcgtcg
      841 agtctgccca tgaggtcgaa tgcgttgcag gcggtgaaat cgttgaagat ggtgagggcg
      901 tcgttcttca tgtattccgc gaacactttg tccttgagtg atgttggcac agagtcggaa
      961 aaggccgtat ccaggatcgt ccgggccgtt gccgccttgt ccctgaggat gccctccagt
     1021 atttcgggat agaccttgag cttcgccccg gtgccgatga ggacgagccc cttcacaagg
     1081 gatggccggg acatggagag acgcatcgcg acagcgccgc ccatggaatg accggcgata
     1141 tgggccgggc cgcaggcgat ggcctcaagg gcgtccccca cagcctgcgc gtactcgtct
     1201 atggaatccg atgagggacc gtccgatcta ccgtggcccg gaaggtcgac gaggattacg
     1261 cggttcgacc tctggagata tgttttctgt gcaagccagg acaacgccga accacccgcg
     1321 ccatggatga agacaacagg ggtgccgctg ccatatgttt ctgtgttcat gatcgcctcc
     1381 cgtgactagc attatagttg atttgccaat ggaattccag agatggacgt ttgaactctt
     1441 gcgcagacag agaaacgggg gtatagttga ggtaaacaca aatggagtga atttcggtga
     1501 ggtctcaaac tcgttgaagg agtgttccat gcaaaagaac gatccggggc cgaaaccagc
     1561 ggccggacga ggacggaagg ctaagacacc gggcacaggg agaacgggtc aggaagcccg
     1621 cgagcaaaga ccatcggtct tagcgacccc cggtgagacg ggaacgccgg acgtgtacga
     1681 cttccatgat cttttccggg cctttatgga caacggcccg gccatagcct ggctcaagga
     1741 tgaggaaggg cgtcatgtct atctcaaccg tgccctgcaa cacaggttct atgatggttc
     1801 gagggattgg tacgggaaga ccgacttcga actctggcct cccgagatgg ccggaaggtt
     1861 ccgggacagg gatcttgccg ttctcggcgc ggagggaacc atcgacgtcg tcgaagaggc
     1921 ggccaatcct gacgggagcc ggtcctggtg gtggaacttt cgctttccat tcgaggattc
     1981 ctcgggcaaa cgatacgtgg gcggtatggg gatagagata tcggagcgca agcgactgga
     2041 agcggaactc agcaagctcg ccgcggttgt gcgccactcc agtgagtttg taagccttgc
     2101 cacgcttgac cggcagatca tttacgtcaa tgagacaggc gccaaaatgg ttggatcgtc
     2161 cccggaaaag atgatcggca tgagtgccct
//
LOCUS       DAOPOA010000412         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_54401_length_2181_cov_4.192380, whole genome shotgun sequence.
ACCESSION   DAOPOA010000412 DAOPOA010000000
VERSION     DAOPOA010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54401_length_2181_cov_4.192380"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..640)
                     /locus_tag="PKJ17_08535"
     CDS             complement(14..640)
                     /locus_tag="PKJ17_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOD76060.1"
                     /translation="MPAGKVIIVLLCALGISIHPGIKGHCGPGGGTGCTTELSAEIEA
                     MKNRNPDKYRDFRHVLRHIRTMNRRQNEKTSLSVAKALVDHVYWERYRFTSAEHRLNF
                     LSVLMGIVRVESLFDPAAVSNRNARGLMQIHSPTWKRYFSSPDEAHDLRRNLTVGTGI
                     LRHYMRRSNNDLRRALYRYLGAPDDRYADKVIESAAAFKARVLSDTME"
     gene            complement(642..1007)
                     /locus_tag="PKJ17_08540"
     CDS             complement(642..1007)
                     /locus_tag="PKJ17_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76061.1"
                     /translation="MTNKNEITEAFIARRSEGFEIVLPRTAIERAARRLAETTNAAGF
                     HDAMELAWFRFDPNVDREMLDCVSMLLTLYRCSLDGNIRPALNLEEVYEHAFNEMEAE
                     HGSLSNKGTPWRSSYCPGG"
     gene            1285..1995
                     /locus_tag="PKJ17_08545"
     CDS             1285..1995
                     /locus_tag="PKJ17_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76062.1"
                     /translation="MERQDGKASDHGIFFVAGIFVLGLLGFFASRSPVVLHRIELPLL
                     RIQAKIYSCCPTGEGHAMELLRIENILAIVDREVGRTGNYGRISTKALGKAMTAASER
                     TKRTTRILSCALVCALGSLVAIRSVRVRRRLHDNRENVRFSRRKGVEGFLQVAGRYLE
                     PDMAVRLGSAPTPEKLADAFGVVRAKVNMPCSVVARLFERGTRERMALLQYGKVRVTF
                     ADEETDGGIRRNKIEKEI"
     gene            2001..2171
                     /locus_tag="PKJ17_08550"
     CDS             2001..2171
                     /locus_tag="PKJ17_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76063.1"
                     /translation="MRMEQGVSPMVTAGERAQAVASYARAFFRSMGADEFALMIENAR
                     SSGQLWLVERLY"
BASE COUNT          448 a          583 c          658 g          492 t
ORIGIN      
        1 cctctccgac cggtcattcc atggtgtccg acaacacgcg ggccttgaac gcggccgcgc
       61 tctcaatgac cttgtcggcg taccggtcgt ccggtgcccc gaggtacctg tatagggccc
      121 ggcgcaggtc attgttcgac cggcgcatgt aatgacggag gatccctgtc cccaccgtga
      181 ggttcctgcg aaggtcatgc gcctcgtcgg gcgaggagaa ataccgcttc cacgttggcg
      241 agtggatctg catgagtccc cgggcgttcc tgttactcac tgcggcgggg tcgaagagac
      301 tctcaacgcg cacgatgccc atgaggacgg aaaggaaatt aagcctgtgc tccgcggaag
      361 tgaaacgata tctttcccaa tatacatggt caacgagcgc cttcgcgacg gagagtgacg
      421 tcttttcgtt ctgtctcctg ttcatcgtgc ggatatgtcg caggacatgg cggaaatccc
      481 ggtatttgtc agggttgcgg tttttcatgg cctcgatctc ggcagacagt tctgttgtgc
      541 acccggtgcc gccgcccggt ccacaatgtc ccttgatgcc ggggtgtata gagatcccta
      601 gagcgcacag caggacgata atgacctttc ctgccggcaa ctcatcctcc ggggcaatag
      661 gaagagcgcc atggcgttcc cttgttcgaa aggctgccat gttcggcttc catctcgttg
      721 aacgcgtgct cgtagacctc ttccaggttc agggcgggcc tgatgtttcc atccagcgag
      781 cagcggtaga gcgtgaggag cattgaaacg cagtcgagca tctccctgtc gacattggga
      841 tcgaatctga accaggcgag ttccatcgcg tcatggaaac cggcagcatt ggtggtttcg
      901 gcaagccgcc tcgcggccct ttctatggcc gtccgcggaa ggactatctc gaaaccttcg
      961 gaccttctcg ctatgaaggc ctcggttatt tcgttcttgt tcgtcataac tctctcatcc
     1021 gatcctttaa acaaaaatag caccgggcgc gggaatctcc gggtgaagac atcgactgct
     1081 caaaaagcga gcgaaggcgg tccgctcttg ctggtcatgg aaatcctccg cctggtcttt
     1141 ttgaaccgca aaagggtctt tttgaagtct ttcatattga caagattgct ttaaatgtac
     1201 tttcatagat ttccaaagta atttcttcgc agaacaatcc atatcgacgc ttcacttctg
     1261 ttgttctcaa gggagggatt tgccgtggaa cggcaagacg gaaaggcctc ggaccatggg
     1321 atattctttg tggccggcat ctttgtgctc ggtcttcttg gcttttttgc ctcgcggagc
     1381 cccgtcgtgc tccacaggat agaattgccg cttcttcgga tacaggcgaa gatctacagt
     1441 tgttgtccca ctggtgaggg ccacgcgatg gaattactga ggatagagaa catccttgcc
     1501 atagtcgata gggaggttgg gaggacgggc aactacggcc ggatcagcac gaaggctctc
     1561 gggaaggcga tgaccgccgc ctcagaacgc acgaagagaa caacacgcat tctctcgtgc
     1621 gcccttgtgt gcgcgctggg ctccctcgtc gcgatacgaa gcgtccgggt gcggcgccgg
     1681 ctccatgaca accgggagaa tgtgcgtttt agcaggagaa agggggttga aggatttctt
     1741 caggtggcag ggagatatct cgagcccgac atggcggttc gcctcggcag cgcaccgaca
     1801 ccggagaagc tcgcagacgc cttcggtgtg gtccgggcga aggtgaacat gccctgctcg
     1861 gttgtcgcgc gactgttcga acgaggcacg cgagagcgca tggccctcct gcaatacgga
     1921 aaggtccgcg tcacctttgc cgatgaagag acggacggag ggatccgtcg gaataagatt
     1981 gaaaaggaga tctgaaagtg atgcgtatgg agcagggagt gtccccgatg gtgactgccg
     2041 gggagcgggc acaggcagtt gcgtcctatg ccagggcctt cttcaggtcc atgggcgccg
     2101 atgagtttgc cctgatgata gaaaacgcca gatcatcggg tcagctgtgg ctggtggaga
     2161 ggctgtatta gagaaaggtc a
//
LOCUS       DAOPOA010000413         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_54444_length_2180_cov_2.572235, whole genome shotgun sequence.
ACCESSION   DAOPOA010000413 DAOPOA010000000
VERSION     DAOPOA010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54444_length_2180_cov_2.572235"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PKJ17_08555"
     CDS             <1..681
                     /locus_tag="PKJ17_08555"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOD76064.1"
                     /translation="INVFRAAWECAPDTCLINPVANCAFPGHLDRYTEDRLWEGNIHR
                     SVISYGSTRRMMLILSECYMMQYGFKTINFFVPNMYGPYDSTDPNKAHALNALIAKAV
                     RIRGEGKKEIEVWGSGIAIREWLYARDFGRIVAETIARINDYGFAEPVNIGQNFGLSV
                     RELAEMILRQAGCACRLSWNRDMPDGAPKKVMDDTRFRKVFPDFEFTALKKGITDTIR
                     YYESIYPY"
     gene            693..>2180
                     /locus_tag="PKJ17_08560"
     CDS             693..>2180
                     /locus_tag="PKJ17_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HOD76065.1"
                     /translation="MENYRNILLYDGIHDVADLVCARDRCDLLYVFDRDRDTTDYLSA
                     LFRNDAGVFIFRGQLDVDMGQVAPGPLPDLLRGTSRNGELYLSILLRDDIARNAAAFE
                     GLAKSGLLRSIEWRIPASAARTGKDDPERLVEGLDRIFPGGHRTDTKRMTVPGGRPAF
                     TVTTWDFDTPAAHGENGTPKGKPPSGHRLLNVVYAHLRTEDIHAVNRGNVSVVWSRFP
                     VEGSDLHLYLNAYSFAGRTEGLDVLLLSEPVIVLPGQYNDDIWQHFDHVITLYDHLIE
                     TRPGFSKNLYPRSGLNVSTDNADAASTADPFERQQKYPLEERLRGMMLVNGNKHSRVP
                     GELYSKRIEAALWFHNNSDLPFHVYGTPPFLLPNYRGAIPVGSRLATVARYRYCLCFE
                     NTDHPVLSLGYVEKILDCMETRTVPVYLGAPNIGRYIPEDCYIDFRRFRDYGELDSYL
                     RSMTDDEYLRYVENIDTWVASGGLSHYTWESLYNRMAGCYAAARGI"
BASE COUNT          468 a          688 c          609 g          415 t
ORIGIN      
        1 atcaacgtat tccgcgccgc atgggaatgc gcccccgata cgtgcctcat caatcccgtt
       61 gccaactgcg ccttcccggg ccacctggac cgctacacgg aggaccgtct ctgggaaggc
      121 aacatccatc gctccgtcat ctcctatgga agcacgagac ggatgatgct gattctcagc
      181 gagtgctaca tgatgcagta cggcttcaag accatcaatt tcttcgttcc caacatgtac
      241 ggtccctacg actcgacgga cccgaacaag gcgcacgccc tcaacgcgct gatagcaaaa
      301 gctgtcagga tccgcggtga ggggaagaaa gagatcgagg tctggggaag cggcatcgcc
      361 atccgcgagt ggctgtatgc cagggacttc ggccgcatcg tggcggaaac gatagcccgg
      421 atcaacgact acggcttcgc cgaacccgtc aacatcgggc agaacttcgg cctctccgtc
      481 cgggagctcg cggagatgat cctcaggcag gccggctgcg cgtgcaggct ttcgtggaac
      541 cgggacatgc ccgacggggc cccgaaaaag gtcatggacg acacccgatt ccgcaaggta
      601 ttccctgatt tcgaatttac agccctcaag aagggcataa cagatacgat ccgttattac
      661 gaatccatct atccttactg aacaggacgg ctatggaaaa ctacaggaac atactcctct
      721 acgacgggat acacgatgtg gcggaccttg tgtgcgcccg cgaccgctgc gatctccttt
      781 acgtcttcga cagggaccgc gacaccacgg actacctgtc ggcgctcttc aggaacgatg
      841 ccggtgtctt cattttccgc ggccagctcg atgtcgacat ggggcaggtc gcgccaggcc
      901 ctctcccgga ccttctcagg ggtacgtcga ggaacggcga actgtacctg tcgatactgc
      961 tccgggatga catcgccagg aatgcggccg ccttcgaggg acttgcaaaa agcggccttc
     1021 tgcgcagcat tgaatggagg atccccgcgt cagcagcccg gaccggcaag gatgaccccg
     1081 agaggcttgt cgagggtctc gacaggatat ttcccggtgg tcatcgaaca gacacgaaac
     1141 ggatgaccgt cccgggaggc cgtccggctt ttacggttac gacctgggac ttcgacacgc
     1201 ctgccgcaca cggggaaaac ggaacgccga aggggaagcc gccatccggc cacaggctcc
     1261 tcaacgttgt gtatgcccac ctgaggacgg aggacatcca cgcggtcaac aggggcaatg
     1321 tctctgttgt ctggtcgcgg tttcccgtgg agggcagcga tctccacctc tatctcaatg
     1381 cctacagctt tgccgggagg acggaaggcc tcgacgtgct cctgctcagt gaacccgtca
     1441 tcgtcctgcc cggtcagtac aatgacgaca tatggcagca tttcgaccac gtgatcaccc
     1501 tctacgacca cctcatagag acgcgccccg gcttctcgaa gaacctttac ccccggtccg
     1561 ggttgaatgt cagcaccgac aacgctgacg cggcctcgac cgccgacccc ttcgagaggc
     1621 aacaaaagta tcctctcgag gaacggctcc ggggcatgat gctcgtcaac gggaacaagc
     1681 actcccgtgt gcccggcgag ctctattcga aaaggatcga agcggccctc tggttccaca
     1741 acaattccga ccttcctttt catgtgtacg gcacgccccc gttcctgctt cccaattacc
     1801 ggggggccat ccccgtgggg tcacggcttg ccacggtcgc ccgctaccgg tattgcctgt
     1861 gcttcgagaa cacggaccat cccgttcttt ccctgggata cgtcgagaag atcctcgatt
     1921 gcatggaaac acgcaccgtg cctgtctatc tcggggcccc gaacatcggg cgctatatcc
     1981 ctgaagactg ctacatcgac tttcgccgct tcagggatta cggagaactc gattcctacc
     2041 tgaggtccat gacggacgac gaataccttc ggtatgtcga gaacatcgac acctgggttg
     2101 cctcgggcgg actaagccac tacacctggg aaagcctcta taacaggatg gcaggatgct
     2161 acgcagccgc cagagggatc
//
LOCUS       DAOPOA010000414         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_54531_length_2177_cov_4.012724, whole genome shotgun sequence.
ACCESSION   DAOPOA010000414 DAOPOA010000000
VERSION     DAOPOA010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54531_length_2177_cov_4.012724"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKJ17_08565"
     CDS             <1..248
                     /locus_tag="PKJ17_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76066.1"
                     /translation="LLYLMDPAVGLYPAWGSLRRLRIFHEPLKPRVCRVTGVSFPCLR
                     DLGLLNTEEIGEPHNLLLVPHTPFEIFLPPEKVKGLG"
     gene            419..1120
                     /locus_tag="PKJ17_08570"
     CDS             419..1120
                     /locus_tag="PKJ17_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD76067.1"
                     /translation="MSMTSTIIRTHDLWKQYETEGHDGMALRGVDLDIGKGQIVVLFG
                     KSGSGKTTLLNLLAGLDEPTRGRIEIDNGDLGTLGEKGRTLLRRHKLGFVFQFFNLLP
                     TLTAEENVLLSLELAGRPDEGLARESLELVGLAGKERRFPHELSGGEQQRVAIARALV
                     KRPPIILADEPTGNLDSATGAQILALLTGRCREAGTTLIMVSHALNTCEYADRVLRMT
                     DGRIFEEISCEGLAP"
     gene            1117..>2177
                     /locus_tag="PKJ17_08575"
     CDS             1117..>2177
                     /locus_tag="PKJ17_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD76068.1"
                     /translation="MRSVIRSFLRYLARRKSLSILQLLGIALGVAAAVGMMLASRSAL
                     ESLGSAVDFLKGGTTHTIARPAGPMEETVLAGIMRDPAVRYFAPVIDRKVRLTGGQQI
                     RMLGLDPFLDRNVRSITARIGASAGDGRTGEAFSSFLFDPGAVLVDENMARGLNLSAG
                     SAIATLQGPLNVVHVFSNPSGEPLMIIDVGHAQKFFRMRGYVDRADLVVTDEEAFRQR
                     WATGFTIESNTQKARNLSALLGAFKLNLEALSLFALFVGIFLIYNTAMFAVVSRRRDA
                     GIFLAIGASRGQVAGAFLVEVLILGVAGGALGGVLGYFLSKFLVEIVGGTISTLYFFL
                     RPSALPWSSWNLAGGVFLGS"
BASE COUNT          405 a          685 c          659 g          428 t
ORIGIN      
        1 ccctccttta cctcatggac cccgcggtgg gtctctatcc ggcatgggga agcctccggc
       61 gcctccggat attccacgaa cccctgaaac cacgggtctg cagggtcacg ggggtcagtt
      121 tcccctgcct cagggacctg ggactcctga acaccgaaga gatcggtgaa ccccacaatc
      181 tgctcctcgt cccgcacacg cctttcgaga tattcctgcc accagagaag gtaaaagggc
      241 tcggatagga cttataggaa cagataggtc ctataggaca tataggatat ataggaccta
      301 taggacgcgc gaaagtcagg tagcactttg ttccccgctt ccgagtctct cctgttggac
      361 atttttgttc atgcggcata tatttgatgt accaaaagga taggtgaggg aatcctcaat
      421 gagcatgaca tctacgataa tacggacgca cgacctgtgg aagcagtatg agacggaggg
      481 ccatgacggg atggctttac ggggtgtcga ccttgacatc gggaaaggtc agatagtggt
      541 tctcttcggg aagagcggct ccgggaagac gacgctgctc aatctgctgg cgggtcttga
      601 cgaaccgacg cgggggcgga tcgagatcga caacggggac ctcggcaccc tgggggaaaa
      661 aggcaggacc ctgctacgcc gtcacaagct gggtttcgtc tttcagttct tcaacctcct
      721 gccgacactc actgcagagg agaacgtcct tctctccctg gaactcgcgg gccgtcccga
      781 cgaaggcctc gcccgtgaat ccctggagct tgtcggcctt gccggcaagg aacgccgatt
      841 tccccatgag ctttccggcg gggaacagca gcgtgtcgcc atagcccgcg ccctcgtcaa
      901 gagacctcca atcatcctcg ccgacgaacc gacggggaac ctggactccg ccacgggggc
      961 gcagatactc gcccttctga ccggccgctg ccgcgaagcc ggcactaccc tcatcatggt
     1021 gagccacgcg ctgaacacct gcgaatacgc cgacagggtc ctcaggatga ccgacggcag
     1081 aatattcgag gagatctcct gcgagggcct cgccccgtga ggagtgtcat ccggtcattc
     1141 ctgcgctacc tggcgcggcg caagagcctc agcatactcc agcttctcgg catagcgctc
     1201 ggggtggccg cagcggtggg gatgatgctc gcctcccgct cggcccttga gagcctggga
     1261 agcgccgtag atttcctgaa gggcgggacg acccatacca tagcgcgccc tgcggggccg
     1321 atggaggaga cggtgctcgc cgggatcatg cgcgatccgg cggtgaggta cttcgccccg
     1381 gtcatcgacc gcaaggtgcg tctgacggga ggccaacaga tacgcatgct gggcctcgac
     1441 cctttcctcg acaggaacgt ccgctccatc acggcccgga taggcgcgtc cgccggtgac
     1501 ggcaggacag gggaggcttt ctcgtcgttc cttttcgatc ccggggcggt ccttgtcgac
     1561 gagaacatgg cgaggggcct gaacctctcg gcgggcagcg ccatcgcaac gctgcagggg
     1621 cccctgaacg tcgtgcacgt cttctccaat ccttccggag agcccctcat gatcatcgat
     1681 gtgggtcatg cccagaagtt cttccgcatg aggggatacg tggaccgcgc cgatctcgtc
     1741 gtcaccgatg aagaggcatt ccggcagcga tgggccacgg gcttcaccat cgaatccaac
     1801 acccagaaag cccggaacct gtcggccctg ctcggcgcct ttaagctcaa tcttgaggcg
     1861 ctgtctctct tcgccctctt cgtgggcatc tttctcatat ataacaccgc catgttcgcc
     1921 gttgtgagca ggcgcaggga cgccggaatc ttcctggcca tcggcgcttc ccggggacag
     1981 gtggccggcg cctttctcgt cgaggtcctt atcctcggtg tggccggcgg cgccctcgga
     2041 ggggtcctcg gctacttcct gagcaagttc ctcgttgaga tcgtgggcgg caccataagc
     2101 accctctact tcttcctgag gccatcggcg cttccctggt cgtcctggaa cctggcgggc
     2161 ggggtgttcc tcggctc
//
LOCUS       DAOPOA010000415         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_54659_length_2173_cov_4.209160, whole genome shotgun sequence.
ACCESSION   DAOPOA010000415 DAOPOA010000000
VERSION     DAOPOA010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54659_length_2173_cov_4.209160"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /gene="flgK"
                     /locus_tag="PKJ17_08580"
     CDS             complement(<1..966)
                     /gene="flgK"
                     /locus_tag="PKJ17_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOD76069.1"
                     /translation="MGITSILNIARNALFAQQTSLQVLSNNIANVNTKGYARQEAVLT
                     EADAVMGDTGLLIGNGVRVDRVASHYDKYLEFTVARQYTALGEQKTYEKFFTRIESVL
                     DETNSKLTSNITAFFNSWQSLSADPLSTVARADVALKATNLCQGIRNTYAQLKNLQVE
                     TDNNVASEVTEINNLTASIAVLNQKTYEAAAGGSESASFTSQRLMALQELSGKLDLQY
                     FEDENGGLTVMTADGKLLVEKGISYELTAEKTGSDNFYHVYWKGNSANSVDITDGIGG
                     GTLKSLIDLRDTQFTSFIDDIDDLARSLMTEVNAIHATGYNPGGTT"
     gene            complement(986..1420)
                     /locus_tag="PKJ17_08585"
     CDS             complement(986..1420)
                     /locus_tag="PKJ17_08585"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOD76070.1"
                     /translation="MMTDAIIEMTKREEKTLKEFLTALQMERDAIISFSLDGIIAGNN
                     KKEEILRKLEYLKAEKEKLLEGAGDREAIVADETMRSLNGQLAVVMKEIKVAMDKNMK
                     LLSFSIDHVRSSIENIIGHISGNMGYGKKREDLSSVLLSKVI"
     gene            complement(1420..1734)
                     /gene="flgM"
                     /locus_tag="PKJ17_08590"
     CDS             complement(1420..1734)
                     /gene="flgM"
                     /locus_tag="PKJ17_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOD76071.1"
                     /translation="MKIQNGNKPDLLDSLVKTAQVKPQQQKDLSVEQRKGQGASYDKV
                     ELSSRKQEVDRLTEKAKTQSVVREEKVEELRARIEDGTYNARGQIVAGSILRSNILDE
                     LL"
     gene            complement(1747..2094)
                     /locus_tag="PKJ17_08595"
     CDS             complement(1747..2094)
                     /locus_tag="PKJ17_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76072.1"
                     /translation="MSESVKSIMAGLPPAISMGIGTMERDLSGSSEADKEKVCRSFES
                     FMIYSMIKHLEKTTKMSKKGYAEETYMAMVYEKVADFLADKGVGIKEMLMKYSDRENA
                     KVIPQSGDNTGKR"
BASE COUNT          384 a          632 c          553 g          604 t
ORIGIN      
        1 cgtggtcccc cccgggttat agccggtagc gtggatggcg ttgacctccg tcatgagtga
       61 ccgcgcaaga tcgtcgatgt cgtcgatgaa ggaggtgaac tgggtgtccc tgagatcgat
      121 gaggctcttc agcgttcctc cgcctatgcc atccgtgatg tcgacggaat tagcggaatt
      181 gcccttccag tagacgtggt agaaattgtc gctgcccgtc ttctcggctg tcagttcgta
      241 tgatatcccc ttttcaacaa ggagcttccc gtcagccgtc atgactgtca gcccgccgtt
      301 ctcgtcctcg aaatactgaa ggtccagttt ccccgaaagc tcctgcagcg ccatgagcct
      361 ctggctcgtg aaactcgcgc tctcgcttcc accggccgcc gcttcatagg tcttctggtt
      421 caacaccgcg atggaggcgg taaggttatt gatctctgtg acctcactgg ccacgttgtt
      481 gtcggtctcc acctggaggt tcttcagctg ggcatacgta ttgcggatac cctggcagag
      541 attggtggct ttcagcgcca cgtcggccct cgcgaccgtg ctcagaggat cggcggacag
      601 gctctgccat gagttgaaaa aggccgtgat attcgacgta agcttggaat tggtctcgtc
      661 gagaacactc tcgatccggg tgaagaactt ctcgtatgtc ttctgttccc ccagcgcggt
      721 atactgcctc gccacggtga attccaggta tttgtcgtag tggctcgcga cccggtcgac
      781 cctcaccccg ttgcctatga gaagcccggt atcgcccatc acggcgtcgg cctcggtgag
      841 gaccgcctcc tggcgcgcgt aacccttcgt gttcacattg gcgatgttgt tcgagaggac
      901 ctgcagcgag gtctgctgtg caaagagggc gtttctagcg atattgagga tagaggtgat
      961 acccattgtt acccttcttc gtgcgttaga tgaccttaga gagaagtacg gaagagaggt
     1021 cctctctctt ttttccgtaa cccatgttgc cgcttatgtg tccgataatg ttctctatcg
     1081 acgatctgac gtgatcgatg gagaaggaga gaagcttcat gttcttgtcc atggcgacct
     1141 ttatctcttt catcaccacg gccagctgtc cgttgaggga ccgcattgtc tcgtcggcca
     1201 cgatggcctc cctgtccccc gcaccctcca gcagcttttc cttttccgcc ttcaggtact
     1261 cgagctttct taagatctct tccttcttgt tgttccccgc gattatgcca tccagtgaaa
     1321 aggatatgat ggcgtcgcgc tccatctgaa gggccgtcag aaactccttc agggttttct
     1381 cctctctctt cgtcatttcg atgatggcgt ccgtcatcat tagagcagct cgtccaggat
     1441 attggatctc agtatgctgc cagcaactat ctggcctcgg gcattgtacg tcccgtcctc
     1501 gatcctggcc cttaactctt cgaccttttc ttcccggacc acggactggg tcttggcctt
     1561 ctccgtaagc cggtccactt cctgcttcct ggatgaaagc tccaccttgt cgtagcttgc
     1621 gccctggccc ttcctctgtt ccacggaaag gtctttctgc tgctgcggct ttacctgagc
     1681 ggtcttgacg aggctgtcaa gcaggtcggg tttgttgccg ttttggatct tcatggtcca
     1741 cctcctctat cgcttaccgg tattatcgcc gctctgagga attactttag cattttccct
     1801 gtcggaatat ttcatgagca tttcttttat gccgacacct ttatccgcaa ggaaatcggc
     1861 aactttttcg tacaccatgg ccatgtaggt ttcctcggca tacccctttt tggacatttt
     1921 cgtcgtcttt tccaggtgct ttatcatgct gtagatcatg aaggactcga agctccggca
     1981 gaccttttcc ttgtccgcct ccgaggaacc tgacagatcg cgttccatcg tgccgatccc
     2041 catcgagatt gcgggcggca aaccagccat tatcgacttt acgctttcag acacagtgag
     2101 cggcctccta tatgacctca aggtcggcat gaagagcgcc tgacgccttg atggtctgca
     2161 gtatcgttat gac
//
LOCUS       DAOPOA010000416         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_54699_length_2172_cov_4.698630, whole genome shotgun sequence.
ACCESSION   DAOPOA010000416 DAOPOA010000000
VERSION     DAOPOA010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54699_length_2172_cov_4.698630"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PKJ17_08600"
     CDS             <1..684
                     /locus_tag="PKJ17_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOD76073.1"
                     /translation="SAYLREKPLFFPNEPVRARQFFGFPLITEDTTFGVIGFVSLNEA
                     ELKESAIGALRNLSSLLSLYFTARWMREHLDRIRDFDPVTGAVQFPVFLDVVETMARK
                     KQRFSVLSVKLGNMILYNRKMGAEGTNDLLRKVCQVIRYCAGGNAFVARKSGGRFFVL
                     LKGGETFETRNMMKLLYHAVGRSLAEQGLSAGAGVVELGTVSFPDDSPDLWELFDRIE
                     EKKIRKFIE"
     gene            697..1728
                     /locus_tag="PKJ17_08605"
     CDS             697..1728
                     /locus_tag="PKJ17_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOD76074.1"
                     /translation="MGFFKNLFGMFSTHLAMDLGTANTLIYMKGEGIVLNQPSVVAID
                     NNSGKVIAVGKEAKEYIGRTPPNISAIRPLKDGVIADFDVAKAMIKYFLKVVSNERKI
                     SKPRMVVGVPSGITQVEKKAVIDACFQVGIRDVHLIEEPMAASIGAGMPIELPRGNMI
                     IDIGGGTTEVAIISLSAVAYSESLRVAGDELDEAIVRYLQKKHQLAIGVIAAEKIKIE
                     GASAFPIDSLSDSINVVGKDLLTSIPRSLKLSKEEVREAIEEPISAIIDSVRRALEKL
                     PAEFVSDLNETGMVLTGGGALLKGLDQRIENETGINVIVAEDPLVSVTMGCGRALEEL
                     AKYKKVFIN"
     gene            complement(1943..>2172)
                     /locus_tag="PKJ17_08610"
     CDS             complement(1943..>2172)
                     /locus_tag="PKJ17_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOD76075.1"
                     /translation="CAEGRFPNPRAIEEGAIEEERRLFYVASTRAMDELYLSYPILAF
                     DRQAGHIIMRPSRFLAELDGARYEEWAISEY"
BASE COUNT          472 a          604 c          665 g          431 t
ORIGIN      
        1 agcgcgtacc tcagggagaa gccgctcttc tttcccaatg aacccgtgag ggcgcggcag
       61 ttcttcggct ttcccctcat aacggaggac acgaccttcg gcgtcatcgg ttttgtgtcc
      121 ctcaacgagg cggagctcaa ggaaagcgcc ataggggcgc tgagaaacct ttcgagcctt
      181 ctttcgctgt atttcaccgc ccggtggatg cgcgagcacc tggacagaat acgcgatttc
      241 gatcccgtca cgggcgccgt gcaatttccc gtcttcctcg atgtcgtcga gacgatggcc
      301 agaaagaaac agcggttctc cgtcctgagc gtgaagctgg ggaacatgat cctttacaac
      361 aggaagatgg gggccgaagg gacgaacgac ctcctgagga aggtctgcca ggtcatccgg
      421 tactgcgccg gggggaacgc cttcgtggcc cgcaagagcg gaggccgttt ctttgtcctt
      481 ctcaagggcg gcgagacctt tgaaacacgg aacatgatga agctcctcta tcacgcggtg
      541 ggcaggagcc ttgccgagca gggactctcg gcgggggcgg gcgtggtgga gctcggaacg
      601 gtcagtttcc ccgatgattc gcctgacctc tgggagcttt tcgacaggat agaagagaag
      661 aagatacgaa aattcatcga ataaggagga agggggatgg gttttttcaa gaatttgttc
      721 gggatgttct ccacgcacct tgccatggac cttggcaccg ccaacaccct gatttacatg
      781 aagggggaag gcatcgtcct caatcagcct tccgtcgtcg ccatcgataa caactccgga
      841 aaggtcatcg ccgtcggcaa ggaggcgaag gaatacatcg gcaggacccc tcccaacatc
      901 agcgccatac ggcccctgaa ggacggggtc atagcggact tcgacgtggc caaggcgatg
      961 atcaagtact tcctcaaggt cgtgagcaac gagcggaaga tatcgaagcc gaggatggtt
     1021 gtcggtgtcc cctcgggcat cacccaggtg gagaagaagg ccgtcatcga cgcctgcttc
     1081 caggtgggca tccgtgacgt ccatctcata gaggagccca tggcggcatc cattggcgcg
     1141 ggcatgccca tagaactgcc acggggcaac atgataatag atatcggcgg cggcacgacg
     1201 gaggttgcca tcataagcct ctccgcggtg gcctacagcg aatctttgcg ggtggccggc
     1261 gacgagctcg acgaggccat tgtccgctat ctccagaaaa agcatcagct tgccataggg
     1321 gtcatcgcgg cggagaagat caagatagaa ggcgcctccg cgttccccat agattccttg
     1381 agcgactcga taaacgtcgt ggggaaggac ctgctcacga gcataccgcg gagcctgaaa
     1441 ctatcgaagg aggaggtccg cgaggccatc gaggaaccca tctcggcgat catcgactcc
     1501 gtgagaaggg ccctcgagaa actgcccgcc gaattcgtct ccgacctcaa cgagacgggc
     1561 atggtgctta ccggcggtgg ggctcttctc aagggactcg accagcggat agagaacgag
     1621 acggggataa acgtgatcgt cgccgaggac cccctcgtat ccgttaccat gggctgcggc
     1681 agggctttgg aagagctggc gaagtacaag aaagtcttta taaactaacc agaagacggc
     1741 ttgaaccgct cgaaccgctc gaaccgctcg agcgtgaaag gaaacggcag aaaaatggcc
     1801 ggaatcgccc cgttgccgtc gatctgtaag tgatcaccgt gttcgcacct ttgggaggtt
     1861 cttttatcct tcccatttct tttctgcgta tcgccgtgaa tggttcgggt tgttctaacg
     1921 ttcaagcggt tcaagcggtc tttcaatatt ccgagattgc ccactcctcg tacctggccc
     1981 cgtcgagctc ggcgaggaag cgggaggggc gcatgatgat gtgaccggcc tggcggtcga
     2041 aggcgaggat ggggtagctg aggtatagct cgtccatggc gcgcgtcgat gctacgtaga
     2101 agaggcgcct ctcctcttcg atggcccctt cctctatcgc cctggggttc gggaaccttc
     2161 cttcggcgca cc
//
LOCUS       DAOPOA010000417         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_54704_length_2172_cov_3.830420, whole genome shotgun sequence.
ACCESSION   DAOPOA010000417 DAOPOA010000000
VERSION     DAOPOA010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54704_length_2172_cov_3.830420"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..473)
                     /locus_tag="PKJ17_08615"
     CDS             complement(93..473)
                     /locus_tag="PKJ17_08615"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOD76076.1"
                     /translation="MPVKLTEAQFDRVVARAIERIPLEIRRYLDNMVIAVKKRPSAGM
                     IKEMGMHPDDYPLGLFQGVPLIERSATTPPLYPDMIYLFQEPLEEMCDTREELEEEIE
                     VTVVHEVAHYIGMTEERLAELGYG"
     gene            complement(510..1085)
                     /locus_tag="PKJ17_08620"
     CDS             complement(510..1085)
                     /locus_tag="PKJ17_08620"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOD76077.1"
                     /translation="MSPSHRVFVPPAGKGPPLAGAVLLLLYPGEDDELFIPLTVRTQN
                     VAVHKGQVSFPGGAHEPQDDSLSHTALREACEELGVCGDDVRIMGALTPLYIEPSNFC
                     VHPFVAWQSYRPSFVPQAIEVSEVLEVPVSHFLDERNVAVEEWLIEGAMRRIPYFGVC
                     GHKVWGATAIILSEFVAVLESLRASGKRDLE"
     gene            complement(1371..1664)
                     /locus_tag="PKJ17_08625"
     CDS             complement(1371..1664)
                     /locus_tag="PKJ17_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76078.1"
                     /translation="MAAVKPKGKSKVTRKKTSTARGSAVDRSFMTALQALEEMARGQI
                     TSAKDPFKTGIRIVGEIKKVILRLENEIEDDSRSMTEAYDSGPGVVGMGIGVL"
     gene            complement(1937..>2172)
                     /locus_tag="PKJ17_08630"
     CDS             complement(1937..>2172)
                     /locus_tag="PKJ17_08630"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HOD76079.1"
                     /translation="GAVLGAVFGAVVAGLPGIIIGPIIGAVAGELLAGRSYREAWLSG
                     WGTFIGFLSGSLVKIIVASLMIGTFIWQVLVAG"
BASE COUNT          469 a          699 c          579 g          425 t
ORIGIN      
        1 cggaatgacg aaagagcacg aatcgtcttc agaaatcact ttgtcaacag cctgttaagc
       61 ggttcaaacc attcaagcaa ctcaaaccgc ctttacccgt atcccagttc cgcgagtctt
      121 tcctcggtca tgcctatgta gtgggccacc tcgtggacaa ccgtgacttc tatctcttct
      181 tccagttctt cccgggtgtc acacatctct tccaggggtt cctggaagag atagatcatg
      241 tcgggataga gcggaggtgt cgtcgcggaa cgctcgataa ggggaacgcc ctggaaaagg
      301 cccaggggat agtcatcggg atgcattccc atctccttta tcatgcccgc cgaagggcgt
      361 ttcttcaccg ctatgaccat attgtccagg taccggcgga tctccagggg gatcctttct
      421 atcgcccggg caaccacgcg atcgaattga gcctcagtga gcttgacggg caccggtggc
      481 ctccttgaaa aagaattgaa cgacggggat cactcaaggt cgcgcttccc cgaggcgcga
      541 aggctctcga ggaccgcgac gaactcgctg aggattatcg ccgtcgctcc ccataccttg
      601 tgcccgcaga caccgaaata gggaatgcgc ctcatagccc cttctatcag ccattcctcg
      661 acggcaacgt tcctctcgtc aagaaagtgg gagaccggca cctcaaggac ctcggagacc
      721 tcgatcgcct ggggaacaaa ggaaggacgg taggactgcc aggcaacgaa ggggtgaaca
      781 cagaaattgc tcggttctat gtacaggggt gtcaacgcgc ccatgatcct cacgtcatcg
      841 ccgcagaccc ccagttcttc gcaagcttca cgaagcgcgg tatgggaaag cgaatcatcc
      901 tgcggctcat gggccccccc gggaaaactg acctgtccct tgtgaaccgc cacattttgc
      961 gtgcggaccg tgagagggat gaagagttcg tcgtcttccc ccgggtacag aaggaggagg
     1021 acggctcccg cgagaggcgg ccccttcccg gcaggcggta cgaacacccg gtgtgatggc
     1081 gacatgcgcc gttgggcgtc atggcccggc agtgccatcc tcaacgcccg ttctatctcg
     1141 tccggtgtga gtctatctcc gatactgtcc tcgcaaaaac taccgccgtc tgcggacaac
     1201 ccgcccgaat acagaccccc gccaggttgg tccgcgcggc acgaaagacc attcaccgtc
     1261 gtggcccttt tcaagcccgg caggaaccag gtttgccgcg tccggggacc cggggaagcg
     1321 gcgatcacgt gtgatctgac ccgctggcgc tcctaagcag acgggtcgct ctacaaaacc
     1381 ccgatgccca tccccacaac gccgggtccg gaatcatacg cctcggtcat tgacctcgag
     1441 tcatcctcga tctcgttctc aaggcgcaga atgaccttct ttatctctcc gacgatcctg
     1501 atccccgtct tgaaaggatc cttcgccgat gtgatctgtc ctctcgccat ctcttccaac
     1561 gcctgcaacg ctgtcatgaa actcctgtcc acggctgaac ccctggccgt cgacgtcttc
     1621 ttccgggtga ccttgctttt ccccttcggt ttcactgccg ccatacaaca cctcctgggt
     1681 aagttagacg tacaatcacc gaatcagctc agagcatgcg ccacctcagc gcgacccctc
     1741 gcagccccaa ggaacgtcca tatttcaatt atattgctaa tacccggttc gtcaagcaaa
     1801 gaaccgtcgc gggtcacagg tcgccaagga aaggcgatgg aagaagtgca ttggatccgg
     1861 cggatctttt tttgccttta gacctgtgac ctgcgacggg ctttcgccag aaaagccctc
     1921 gggccgggaa ccacgctcat cccgcgacga gcacctgcca gatgaaggta cctatcatga
     1981 gagaggcaac gatgatcttg acaaggcttc ccgacagaaa accgatgaaa gtgccccaac
     2041 ccgacagcca ggcctcgcgg taggaccgcc cggccaggag ttctcccgcg acggccccga
     2101 taatggggcc gataatgatg cccggcaggc cggcgacaac ggccccgaag accgcaccca
     2161 gcaccgcgcc cc
//
LOCUS       DAOPOA010000418         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_54870_length_2167_cov_3.107008, whole genome shotgun sequence.
ACCESSION   DAOPOA010000418 DAOPOA010000000
VERSION     DAOPOA010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54870_length_2167_cov_3.107008"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKJ17_08635"
     CDS             complement(<1..549)
                     /locus_tag="PKJ17_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD76080.1"
                     /translation="MKSLPTDKRSLIPIILCGGAGERLWPLSRDEYPKQLLPLVGPET
                     MLLETVRRMAGFVDDNVELASAPMIVCNEDHRFLVAGQLKSMALEGPPIILEPCGRNT
                     APALTVAALYAARNGQDPLLLVMPSDHVVEDRMALHHSVNLGIGPALAGAIVTFGIVP
                     DRPETGYGYIEADRQAPVAQHVF"
     gene            complement(550..1662)
                     /locus_tag="PKJ17_08640"
     CDS             complement(550..1662)
                     /locus_tag="PKJ17_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOD76081.1"
                     /translation="MDKLQGKAITVTGGKGFLGQHLIRTLLSSGCRNVMVADLPEYDL
                     VSLADVKRMYEDLNPDIVIHLAAKVGGIGFNQENPASLFYENAMMGIQLVHEGYAHSI
                     EKFVALGTICAYPKFTPVPFREDDIWNGYPEETNAPYGLAKKMMLVQSQAYRQQYGFN
                     SIFLLPVNLYGPGDNFSFRSSHVIPALIRKFHLANLLARGDFDAIAADFEWNKDAEVI
                     IDNSLMAITQASSKTDILKVLEHYGISHSLSHSSRSVVEIWGTGSATREFFYVEDAAE
                     AIVAATKSYDKSDPVNIGAGFEISIKDLVTLIVNLMRFDGEVTWDRSKPDGQPRRMLD
                     TTKARMEFDFTARTSFREGLVKTIEWYTKGEEGHRA"
     gene            complement(1668..>2167)
                     /locus_tag="PKJ17_08645"
     CDS             complement(1668..>2167)
                     /locus_tag="PKJ17_08645"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOD76082.1"
                     /translation="RRDWGFAPEYVEMMWLMLQRDEPDDYVVGTGESHSVREMIEVAF
                     GYAGIDLEWRGEGESETGVISSLRTGIDTHLKPGDVVIEIDPRYFRPTEVTHLQADIS
                     KARMQLNWEPRITFPELTRIMVDYDLRMAGIEAPCEGIEACRAKGFCYTDHKYALYER
                     IREGA"
BASE COUNT          447 a          592 c          629 g          499 t
ORIGIN      
        1 gaaaacgtgt tgggcgaccg gggcctgccg gtcggcctca atgtagccat agccggtttc
       61 cgggcggtcg gggacgatgc cgaaggtgac gatggcgccc gcgagggccg gtccgatgcc
      121 caggttcacg gagtgatgga gggccatgcg gtcttccacg acatggtccg aaggcatgac
      181 gaggagcagg gggtcctgtc cgttccttgc tgcataaagc gcggcgacgg tcagtgccgg
      241 ggcagtgttg cggccgcagg gctcgagaat gatgggcggg ccctcaaggg ccatgctctt
      301 tagctgcccg gcaaccaaga atcgatggtc ttcgttgcag acgatcatag gggcagatgc
      361 aagctccaca ttgtcatcga caaaacctgc catgcgacgg accgtttcca gaagcatcgt
      421 ttcgggcccg accagaggta aaagttgttt ggggtactca tcgcgggaca ggggccagag
      481 cctctctccc gctccgccgc ataggatgat agggataagg gatcgtttat cagtcggtag
      541 ggatttcatt caagcacgat gaccttcttc gcctttagtg taccattcta ttgtctttac
      601 caatccttcc ctgaaagagg tccgggccgt aaagtcaaat tccattcttg ccttcgtggt
      661 atccagcatt cttcttggct gaccgtccgg tttggagcga tcccacgtca cttcaccatc
      721 aaatctcatc aggttgacga tgagggtgac gaggtctttg atggagatct cgaacccggc
      781 gccgatattg accgggtcgc tcttatcgta gcttttcgtg gccgcgacga tggcttcggc
      841 cgcatcttcc acgtagaaga actcgcgggt ggcggaaccg gtgccccaaa tctcgacaac
      901 ggaccgtgag gagtgagaca acgaatgaga gatgccgtaa tgttccagta cttttaggat
      961 gtcggttttt gatgaggctt gggtgattgc catcagggag ttgtcaatta tgacttcggc
     1021 atctttgttc cactcaaagt ccgccgctat tgcgtcaaaa tctccgcggg ccaggagatt
     1081 ggcgaggtgg aattttctga tgagtgccgg gatgacgtgg gacgacctga agctgaaatt
     1141 gtcccccggg ccgtaaaggt tgactggaag gagaaaaatg gaattaaagc cgtactgctg
     1201 cctataggcc tgagactgga cgagcatcat cttcttggca aggccgtatg gtgcgttggt
     1261 ctcttcggga taaccgttcc atatatcgtc ttcccggaag ggaacggggg taaacttggg
     1321 ataggcgcaa atggtcccga gggcaacaaa cttctcgatg ctgtgggcat acccctcatg
     1381 tacgagctgg atgcccatca tggcgttctc atagaagagg gaagccgggt tttcctggtt
     1441 gaacccgatg ccgccgacct ttgcggcgag gtggatgacg atatcggggt tcaagtcttc
     1501 gtacatgcgc tttacgtccg caaggctgac aaggtcgtac tcgggaaggt cagcgaccat
     1561 gacattccga cagccgctcg agaggagcgt gcggatgagg tgttggccca gaaacccttt
     1621 cccgccggta acggtaatgg ctttaccctg aagcttgtcc aagtcgatca ggccccctcc
     1681 cttatccttt cgtagagggc atacttatga tcggtgtaac agaaaccctt cgcacggcat
     1741 gcctcgatac cttcgcaggg cgcttcgatg ccggccatcc ggagatcgta gtcgaccatt
     1801 atcctggtaa gttccgggaa cgtgatcctc ggctcccagt tcaactgcat cctggccttc
     1861 gagatgtccg cctgaagatg ggtcacctcc gtaggcctga aatacctcgg gtctatctcg
     1921 atgacaacat cccccggttt cagatgcgta tcgatgcctg tcctgagaga cgagatgaca
     1981 cccgtctctg actcgccctc tccacgccat tcgaggtcta tcccggcgta tccgaaggcc
     2041 acctcgatca tctccctgac ggaatggctt tcgcctgtcc cgacaacgta gtcatccggt
     2101 tcgtcccgct ggagcataag ccacatcatc tctacatatt ccggggcaaa cccccagtct
     2161 ctcctgg
//
LOCUS       DAOPOA010000419         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_55142_length_2159_cov_3.866445, whole genome shotgun sequence.
ACCESSION   DAOPOA010000419 DAOPOA010000000
VERSION     DAOPOA010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55142_length_2159_cov_3.866445"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="PKJ17_08650"
     CDS             complement(<1..1802)
                     /locus_tag="PKJ17_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD76083.1"
                     /translation="MKDQEISGKTEKGKRCGPDPAGEYQDVRALLEDSEARYRLLVKH
                     SSDGVFLFNPRTLKILEANASFLKTTGFREEEITRLTLLDITAMKKKEILQNVRGVLR
                     DAEIVSGLRQYRRKDGTLIDVEITSSLINYRNDQFIMVNVRDVTERLRAQHELERQAR
                     MLREQAEIIDIAEDAIIVRDMKNRVVFWNRGAANRFGFSMDEAWGKPLSSLLKTRYPE
                     PLSTIRKRLLAEGKWEGELVHRTKDGRRIIVESKWKLRTDKRNRPATVLQIDNDITER
                     KLAEEVLRRSKTELERRVAERTSEITEANARLVQELRRRQQVEDMLRRGAERYRNLFE
                     NSPLGIYRTDPQGKILMANPALIRMMGYSSFADFGAVKSYRKSCEPTYLGRKIKDRLE
                     REGRVRGFEARWKMRDGSQMHVRENARAIRDSSGRTLYYEGTVEDISEQKQAEERLDQ
                     YQRQLRSLASELSLAEERERRRISNLLHDHIGQLLATAKMRLGWIESAVKDENIEREI
                     DQIRDHIGQAIQFGRSLTFELSPPILYDLGLEAALEWLAEQVENQNGIHREFESDGLE
                     KPIRDEIRVLLFTAVRELLANVVKHSGATRVKVTV"
     gene            complement(1974..>2159)
                     /locus_tag="PKJ17_08655"
     CDS             complement(1974..>2159)
                     /locus_tag="PKJ17_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76084.1"
                     /translation="MMSIIQALLIYGMIVVGDPLDATGHYGVSCTGAPDEKTVENAGK
                     LGKRVATLALALKKRQA"
BASE COUNT          355 a          694 c          593 g          517 t
ORIGIN      
        1 accgtcacct tcacgcgcgt cgctccggaa tgcttgacca cgttcgcaag gagttcccga
       61 acggcggtga agaggagcac ccggatctcg tcccgtatgg gcttttcaag gccatcgctc
      121 tcaaactccc tgtggatccc gttctggttc tcaacctgct ccgccagcca ctcgagggcg
      181 gcctcgagcc ccagatcgta caggatcggg gggctgagct cgaaggtgag cgaccggccg
      241 aactggatgg cctgcccgat gtgatcgcgg atctggtcta tctccctttc gatgttctcg
      301 tccttgacgg cgctctcgat ccatccgagc ctcatcttgg cggtggcgag aagttggcct
      361 atgtggtcat ggaggaggtt cgatattcgt ctgcgctccc gttcctcggc aagagagagc
      421 tccgacgcca gggagcggag ctggcgctgg tattggtcca atctctcctc cgcctgtttc
      481 tgctcgctga tatcttcaac ggtgccttca tagtagaggg tccgcccgct ggagtcccgt
      541 atggcccgcg cgttctcccg cacatgcatt tgcgacccgt cgcgcatttt ccaccgggct
      601 tcgaaaccac ggacgcggcc ctcccgctcc agccgatcct ttatcttcct gccgaggtag
      661 gtgggctcac agctctttcg gtaggacttg acggctccga agtcggcgaa ggaggaatag
      721 cccatcatgc ggatcagggc cgggttcgcc atgaggatct tcccctgcgg gtctgtccgg
      781 tatatgccga gcggtgagtt ctcgaagagg ttcctgtaac gctcggcccc ccttctcagc
      841 atgtcctcga cctgctggcg tcttctcagt tcctgcacga ggcgggcgtt ggcctccgtt
      901 atctcgctcg ttcgctccgc cacgcgccgc tccaattccg tctttgagcg cctcaaaacc
      961 tcctcggcga gcttccgctc cgtgatgtca ttgtctatct gaagcaccgt tgcgggcctg
     1021 tttctcttgt ccgtcctgag cttccatttg ctctccacga tgatccgcct gccgtccttc
     1081 gtacgatgga ccagctcccc ttcccatttt ccctcggcca gaaggcgttt ccgtatggtg
     1141 ctgagtggtt ctgggtaccg cgtcttgaga agcgacgaca gcggttttcc ccaggcctcg
     1201 tccatcgaaa agccgaagcg gttcgccgcc cccctattcc agaagactac ccggttcttc
     1261 atgtcgcgga caatgatggc atcttcagcg atatcgatga tctccgcctg ctcccgaagc
     1321 attctcgcct gccgttcaag ctcatgctgc gcccggagcc gctccgtgac gtcgcggacg
     1381 tttaccatga tgaattggtc gtttcggtaa tttatgaggg aagacgtgat ctccacatcg
     1441 atgagagtgc cgtcttttct tctgtactgg cgcagtccgg agacgatctc ggcgtccctg
     1501 agcacacccc ggacgttctg aagtatctcc ttcttcttca tggccgtgat gtcgagcagc
     1561 gtgagccggg ttatttcctc ctcgcggaag ccggttgtct tgagaaagct ggcatttgcc
     1621 tcaaggatct tcaacgtccg gggattgaag aggaagacgc cgtccgacga gtgcttaacg
     1681 agcagacggt accgggcctc gctgtcctcg agcagggctc tgacgtcctg gtattcccct
     1741 gccgggtcgg gcccgcatcg ttttcccttc tcagttttac cggatatctc ctggtccttc
     1801 attttatatc agcacctgat cgatattgac catttttgcg cgccagacac caggtcggga
     1861 tcacaaaagg atgccggggg atcccccgaa ggaaaacccc cgcatcgcgc atcggtcctt
     1921 ccggctttcg gacccggtca gagcccgtcc cgccggtgtt ccacgtgaaa cgctcacgct
     1981 tgccgcttct tcaacgccag cgccagggtt gccactctct tccccagctt gccggcattc
     2041 tcaaccgtct tttcatcggg agcgcccgta cacgatacac cataatggcc cgtggcatca
     2101 agagggtcgc cgacgacgat catgccgtag atgagcaggg cctggatgat cgacatcat
//
LOCUS       DAOPOA010000420         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_55329_length_2154_cov_3.916151, whole genome shotgun sequence.
ACCESSION   DAOPOA010000420 DAOPOA010000000
VERSION     DAOPOA010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55329_length_2154_cov_3.916151"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKJ17_08660"
     CDS             <1..443
                     /locus_tag="PKJ17_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76085.1"
                     /translation="FDIILTGVQSEDDQFGAVGGIAAALMGLPYASMVIGIDAVEADH
                     VVIRRELEGGLQEKVKVTVPCVLSIQSGINEPRYVSIMGIRKASKVERKVFKADGYEK
                     GSPKIELTKWVYPEKKGGATMLDGELADVCGQLLGILKEKGVCQ"
     gene            440..1372
                     /locus_tag="PKJ17_08665"
     CDS             440..1372
                     /locus_tag="PKJ17_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOD76086.1"
                     /translation="MSYTLIVAEVRRGVFEERNLDSLGLGALLGAQSVLLVPDGAYQV
                     PDKFVDAIVKVKAAEEVYLNPMNMGRMLEAAFAAKGKPDHILFTQSSSGMEAAAYAAG
                     LFGMPVITDVSGYDRDAGIFLKSYYSDKVFGQFKAAGSPAILTVRSGSFREFAAEKTA
                     AAAAEAIDAAAVETRTFVEYVEEEKTDIDITKAEFLLSVGRGVGNQGEVSGYEELAGI
                     LKAVLSGSRPVIDKMWLPKARQVGTSGKTVKPKVYLAMGISGAFQHIAGMKDSDCIIA
                     VNKDPEAPIFQYAHYGIVGDIHKVRDKLKELAKG"
     gene            1372..>2154
                     /locus_tag="PKJ17_08670"
     CDS             1372..>2154
                     /locus_tag="PKJ17_08670"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD76087.1"
                     /translation="MDEKETKVLVVGGGIGGITAALEIASCGINVTMVEESPSIGGRM
                     IQLDKTFPTLDCSTCVLSPKMVEVALNQKIDLLSWAKVMAVKKEGRGFNATILKRSRY
                     VDLKKCTACGVCSGGCPVVMKNEFNVGTGPRKAVYIPFPQAIPNKALIDKREERLCNM
                     ACMDACPVNTNVPGYLKHISEGRFADAYRLIRATNPFPSVCGRVCYAPCERVCNRGEI
                     DEPLAIRDLKRFAVDNFDIATLDVPQVQRMGRKVAVVGAGPAG"
BASE COUNT          456 a          618 c          709 g          371 t
ORIGIN      
        1 ccttcgatat catcctgaca ggtgtgcagt cggaagacga ccagttcgga gccgtcggag
       61 gcattgccgc cgccctgatg ggtcttccct atgcgtcgat ggtgatcggc atcgatgccg
      121 tcgaagcgga ccacgtcgtg atccgcaggg agctcgaagg cggcctccag gagaaggtga
      181 aggtgaccgt cccttgcgtg ctttcgatac agagcggcat caacgagccc cggtacgtct
      241 ccatcatggg catccgcaag gcctcgaagg tggagcgcaa ggttttcaag gccgatggat
      301 acgagaaggg ctctccgaag atcgaactga cgaaatgggt ctatcctgag aagaagggtg
      361 gggcgacaat gctcgacgga gagctcgccg acgtgtgcgg gcagctcctc gggatcctca
      421 aggagaaggg ggtgtgccaa tgagttacac attgatcgtg gcagaggtga ggcgcggcgt
      481 attcgaggag aggaacctcg actcgctggg ccttggtgcc cttctcggag cgcagtcggt
      541 ccttctcgtc cccgacgggg cctatcaggt gccggacaag ttcgtcgacg ccatcgtgaa
      601 ggtaaaggca gccgaagagg tgtacctcaa tcccatgaac atgggcagga tgctggaagc
      661 cgcctttgcc gcgaaaggca agccggacca catcctcttc acccagtcct catcggggat
      721 ggaagctgcg gcatacgccg cgggcctttt cggaatgccc gtgataacag atgtgagcgg
      781 ttatgacagg gatgccggca tcttcctgaa gagctactac tccgataagg tcttcgggca
      841 gttcaaggcc gcgggctctc ccgccatact caccgtgagg agcgggagct tccgggagtt
      901 cgccgcggaa aagaccgccg ccgctgcagc tgaggccatc gacgccgccg ccgtcgagac
      961 ccgcacgttc gttgaatacg tcgaggaaga gaagacggac atcgacatca cgaaggcgga
     1021 gttcctcctt tccgtgggaa ggggcgtcgg caaccagggc gaggtctccg gctacgagga
     1081 gctcgcgggc atcctgaagg cggtcctttc cggatcgcgt cccgtcatcg acaagatgtg
     1141 gctgcccaag gcgcggcagg tggggacgtc gggcaagacg gtgaagccga aggtatacct
     1201 tgccatgggc atatcgggcg ccttccagca catcgcgggc atgaaggact cggactgcat
     1261 catcgcagtc aacaaggacc cggaggcccc catcttccag tatgcccatt acggcatcgt
     1321 cggggacatc cacaaggtgc gtgacaaact aaaagagctg gcaaagggct gatggacgaa
     1381 aaagaaacaa aagttctcgt ggtgggtgga gggataggcg gcatcaccgc cgccctggag
     1441 atcgcctcct gcggcatcaa tgtgaccatg gtggaagaga gtccctccat cgggggcagg
     1501 atgatacagc tcgacaagac cttccccacc ctcgactgtt ccacctgcgt cctttcgccg
     1561 aagatggtgg aggtcgcgct gaaccagaag atagacctcc tgtcatgggc gaaggtcatg
     1621 gcggtgaaga aggagggcag ggggttcaac gccaccatcc tgaaaaggtc ccggtacgtg
     1681 gacctgaaga aatgcaccgc ctgcggcgtc tgttccggag gctgtccggt ggtgatgaag
     1741 aacgagttca acgtgggaac gggaccgcgg aaggccgtct acataccctt tccccaggcc
     1801 attcccaaca aggccctcat agacaagcgg gaggagcgcc tgtgcaacat ggcctgcatg
     1861 gacgcctgtc ccgtcaacac caatgtgccc ggctacctga agcacatcag tgaggggagg
     1921 tttgccgacg cctacaggct catcagggcg acgaacccct tcccgtcggt ctgcggcagg
     1981 gtctgttacg ccccctgcga gagggtgtgc aacaggggcg agatcgatga gccgctggcg
     2041 atccgggacc tcaagcgctt cgccgtcgac aacttcgaca tcgccaccct ggatgtcccc
     2101 caggtgcaga ggatgggcag gaaggtggcc gtggtcggcg ccggcccggc gggg
//
LOCUS       DAOPOA010000421         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_55357_length_2153_cov_3.942326, whole genome shotgun sequence.
ACCESSION   DAOPOA010000421 DAOPOA010000000
VERSION     DAOPOA010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55357_length_2153_cov_3.942326"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PKJ17_08675"
     CDS             <1..604
                     /locus_tag="PKJ17_08675"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOD76088.1"
                     /translation="IISRGVTDRRVINAMLKVPRHLFVDEALWPEACEDHPLPIGEKQ
                     TISQPYIVALMTELLRLTGKEKVLEIGTGSGYQCAVLAEVADMVYTIERIPAIAKRAR
                     KIFDQLKYTNVIIHIGDGTLGWKEHSPYDGIIVTAAAPHPPAALLEQLAIGGRLVIPI
                     GNEFSQDLIIFTREAKDSYTEENYGGCRFVKLIGQQGWRE"
     gene            601..1467
                     /locus_tag="PKJ17_08680"
     CDS             601..1467
                     /locus_tag="PKJ17_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD76089.1"
                     /translation="MNGEKDTPDFARYDDIEVERLYMRDLRKLPVISVEEEKMYARMV
                     AEGSPEARKKMIEANLRLVVKIARRYVNQGMSLLDLIEEGNIGLIKAVEKFDLAKECR
                     FSTYATWWIKQSIERSIANHSRTIRLPVHVSSRVNRISKIISTTVEKTGREPSLEEIS
                     SESGYRIDFVRNLFTMAIKTYSLESYIDENSKLTLEEVIPNPDNEEPLSILEHTRRME
                     EVASWLDTLASDERKVIMLRFGLDGDEPQTLESIGKTFGVTRERIRQIEQKALNKLRK
                     IVKRRNIGTESI"
     gene            1448..>2153
                     /locus_tag="PKJ17_08685"
     CDS             1448..>2153
                     /locus_tag="PKJ17_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOD76090.1"
                     /translation="MEQKASESSRRNPALDIIRKGSVITIITLVSRPLGYVREAIQAY
                     LFGATLLVDAFIVAFNFPELIQTLFFSGATSAFLIPVCTKYLHDREEYSRIYSTFINI
                     SVVLTLFVSLLFFFFSGTVVRMIAPGFTEEARAITNNLFLIMIPVIAFHTVLSVMKAF
                     LNAKEHFAAPELSGILWNLFFIASAVLLAKKLGIYSLAIGVSVGSLLQIVMQYPYLRK
                     FGIRYHFSLDLGHPAVA"
BASE COUNT          497 a          682 c          565 g          409 t
ORIGIN      
        1 gatcatctcc cgcggcgtta cggaccggcg cgtcatcaac gcgatgctga aggtcccgag
       61 gcacctcttc gtcgatgagg ccctctggcc ggaggcctgc gaggaccacc ctcttcccat
      121 cggcgagaag cagaccatct cgcagcccta catcgtggcc ctcatgacgg aactcctccg
      181 tctcacgggt aaggaaaagg tcctcgagat cggcaccggg tcggggtatc agtgcgccgt
      241 cctcgctgag gttgccgaca tggtctacac catagagcgc atacccgcca tagcaaaaag
      301 ggcgcgcaag atattcgacc agttaaagta caccaacgtg atcatccaca tcggcgacgg
      361 cactctcggc tggaaggaac acagccccta cgacggcatc atcgtgacag ccgccgcccc
      421 gcatcctccc gcggccctcc tggaacagct cgccatcggc ggcaggcttg tcattcccat
      481 tggaaacgag ttctcccagg acctcatcat cttcacccgt gaggccaaag acagttatac
      541 agaagagaat tacggcggct gcaggtttgt caagctcatc ggccaacagg gatggagaga
      601 atgaacgggg aaaaggacac gccggatttc gcacgctacg acgatatcga ggtggaacgc
      661 ctctacatga gggacctgcg caagctgccc gtcatctccg tcgaagaaga gaagatgtac
      721 gcccgcatgg tggctgaagg gagccccgaa gcccgcaaga agatgataga ggccaacctg
      781 cgtctcgtgg tgaagatcgc ccgcaggtac gtcaaccagg gaatgagcct tctcgacctc
      841 atcgaggagg gcaacatagg cctcatcaag gcggtggaaa aattcgacct ggcgaaggag
      901 tgccgcttct ccacgtatgc cacgtggtgg ataaagcagt ccatcgagcg gtcgatagcg
      961 aaccactccc ggaccatacg gctgcccgtc cacgtctcat cccgggtgaa caggatatcg
     1021 aagatcatca gcaccaccgt tgaaaagacg ggccgggagc cttcgctgga agagatatcc
     1081 agcgaatcgg gataccgcat cgacttcgtc aggaacctct tcaccatggc gataaagacc
     1141 tactctctcg agagctatat cgacgagaac tcgaagctca ccctcgagga ggtcattccc
     1201 aaccccgaca acgaagagcc gctttccatc ctggagcaca caaggcgcat ggaggaggtg
     1261 gcctcgtggc ttgacaccct ggcctccgat gagagaaagg tcataatgct gcgcttcggc
     1321 ctcgacgggg acgaaccgca gacactggaa tccatcggca aaaccttcgg cgtgacgcgg
     1381 gagcgcatac gccagataga acagaaggcc ctcaacaagc tccgcaagat cgtcaagaga
     1441 cggaatattg gaacagaaag catctgagtc ctcccgccgg aatccggcac tggacatcat
     1501 ccgcaaaggc tccgtcatca ccatcataac cctcgtttcg agaccgctgg gttacgtgcg
     1561 cgaagcgata caggcatacc tcttcggggc cactctgctc gtcgacgcct tcatcgtggc
     1621 cttcaatttt cccgagctca tccagacgct cttcttttcc ggggcaacga gcgcctttct
     1681 catccccgtg tgcacgaagt acctccacga ccgcgaggaa tattcccgca tatactcgac
     1741 gttcataaac atctccgttg tcctcacgct cttcgtctcc ctgctgttct tttttttcag
     1801 cggcaccgtc gtgcggatga tcgcacccgg cttcaccgaa gaggcgaggg cgatcacgaa
     1861 caacctcttc ctcatcatga tacccgtcat agccttccac acggtccttt ccgtcatgaa
     1921 ggcattcctg aacgcgaagg aacacttcgc tgctccggaa ttgtcgggga tactgtggaa
     1981 cctcttcttc atcgcctcgg cagtcctcct ggcaaaaaag ctcggcatct acagccttgc
     2041 cataggcgtc agcgtggggt ccctgctgca gatcgtcatg cagtatcctt atctcaggaa
     2101 attcggcatc cgataccact tctccctcga tctcggccac cccgccgtcg cca
//
LOCUS       DAOPOA010000422         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_55367_length_2153_cov_3.160629, whole genome shotgun sequence.
ACCESSION   DAOPOA010000422 DAOPOA010000000
VERSION     DAOPOA010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55367_length_2153_cov_3.160629"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1819)
                     /locus_tag="PKJ17_08690"
     CDS             complement(68..1819)
                     /locus_tag="PKJ17_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOD76091.1"
                     /translation="MARVISFKTGRRTPTLFRLLRDRYLKDGIDDGYIAFKWEEWGKN
                     PSITVFRAVKNNRVVGWIFYDRKTSTIEEMLVEGTWKGKDPRPEMLDTLIARESLVAA
                     SLLTADQEKYELLIGYGFRPTLFFHRNGFDLVRMELSTSVLLKKTAAGKPFRGYRKRE
                     RVAVEKIPPTQTYEEIKKGLAGLIDRLGGLRRFVKPGQTVAIKPNVVSDHGLKDGKVV
                     GGIVTDIRVVKALTEMLLGLASHVYITEGASINRSATSKMFSHYGYDEIVALDPAKIS
                     LVDLNTDRLIDKPVPGCKRMSSRKIPATLEMVDVIINVPVLKIHFAAISSLAIKSLQG
                     AVPPIEKYMSHFFGLWQSLVNIHHLVKPKLTIIDGLTGLEDFGPVSGTPIKMDVLIGG
                     TNAVAVDAVAMEIMGIDPRTSPPVFLAWMQGLGPLEKNKINVVGTPIEEVAKKFVQPA
                     IDVSGGASLKILADEACPGCKGYLHFVLSKLRRPDPADPTRSLIDRPLEKKANIFLGP
                     STTVPVNPDETNIFMGICQQHHAGLGTHMPGCPPHAEVITKAVYSLFPDIEPPKYADE
                     TEETKLGKMLEEILQNR"
     gene            complement(1931..>2153)
                     /locus_tag="PKJ17_08695"
     CDS             complement(1931..>2153)
                     /locus_tag="PKJ17_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76092.1"
                     /translation="KSPEDGMIDPVSSIMSYAMSRNEATMSLAKSRLEAEAAVAQVIL
                     EAAKNIQRMTEVSSSSASPFLGSKIDVHV"
BASE COUNT          393 a          593 c          665 g          502 t
ORIGIN      
        1 ggaaaagagg gccggctggt ggggtttatc aacaggctgt caagcgattc aagccttcaa
       61 accgtcttta ccggttctgc agtatttcct ccagcatttt ccccagtttt gtttcttccg
      121 tttcgtcggc atacttgggg ggctctatgt cgggaaaaag gctgtagacg gccttggtga
      181 tgacctccgc gtggggaggg cagccgggca tgtgggtgcc gagaccggcg tggtgctgct
      241 ggcagatacc catgaagatg ttcgtctcgt cggggttgac gggaacggtt gtcgatgggc
      301 ccaggaagat gttggccttc ttttcgaggg gcctgtcgat gagagaccgg gtggggtcag
      361 cgggatcggg cctgcgcagc ttggaaagga cgaagtgcag ataacccttg catcccgggc
      421 acgcctcgtc ggcaaggatc ttgagcgagg cgccgccgct gacatcgatg gcgggctgca
      481 cgaatttctt cgccacctct tcaatggggg tgccgacgac gttgatcttg ttcttctcga
      541 gcggtcccag gccctgcatc caggcgagga ataccggggg agatgtcctc gggtcgatac
      601 ccatgatctc catggctacg gcatcgacgg ccacggcgtt cgttccgcct atcaggacat
      661 ccatctttat cggggtgccc gagacgggcc cgaagtcctc gaggcctgtg aggccgtcga
      721 tgatggtcag tttcggcttg acgaggtggt ggatgttcac gaggctctgc cagagaccga
      781 aaaagtggga catgtatttc tctatgggcg gtacggcacc ctgaagactc ttgatggcca
      841 gcgaggaaat ggcggcgaaa tggatcttca gcaccggcac attgatgatg acgtcgacca
      901 tttcaagggt agcggggatc tttcgggatg acatgcgttt gcagcccgga accggcttat
      961 cgatgagcct gtccgtgttg aggtcgacga gactgatctt cgcggggtcg agggcgacga
     1021 tctcgtcata tccataatgg ctgaacatct tcgacgtcgc gctgcggttg atcgaagcgc
     1081 cttcggtgat gtacacgtgg gatgcgagac cgaggagcat ttccgtcagg gctttgacga
     1141 cacggatgtc ggtgacgatg ccgcccacga cctttccgtc cttaaggcca tggtcgctca
     1201 cgacgttggg cttgatcgcg acggtctgcc cgggtttgac gaaccgcctg agcccgccga
     1261 gcctgtcgat gagaccggca agtcctttct tgatctcttc gtatgtctgg gtgggtggta
     1321 tcttctccac ggcgacgcgt tccctctttc gataaccgcg gaatggtttg cccgcggccg
     1381 tcttcttcag cagcaccgac gtgctcagtt ccatcctgac caggtcgaaa ccgttgcggt
     1441 ggaagaaaag cgtgggcctg aaaccatacc cgataagaag ctcgtacttc tcctggtccg
     1501 ccgtgagaag agatgccgct acgaggcttt cgcgggcgat gagcgtgtcg agcatctccg
     1561 ggcgggggtc cttgcctttc cacgttccct ccacgagcat ctcctcgatg gtgctcgtct
     1621 ttctgtcgta gaagatccat cccacgaccc tgttgttctt cacggcgcgg aagaccgtga
     1681 tggaagggtt ctttccccac tcttcccatt tgaaggcgat ataaccgtcg tcgatgccgt
     1741 ccttcagata acggtctctg aggagcctga aaagggtggg ggttcgcctt cccgttttga
     1801 aagatattac tcttgccatg ctgttgacct cctcctccgt gcgagtgtgg atcctacccc
     1861 gcccccgtca cacgtgccaa gattatatca gtttccgggc tagcgggaaa gaggttaggc
     1921 gttccgcgct ctaaacgtgg acatcgatct ttgatccgag aaaaggcgag gcggaggacg
     1981 aggacacctc ggtcatgcgc tggatgttct ttgcggcctc cagtatgacc tgcgcaaccg
     2041 ccgcctccgc ctccaaccgg ctcttcgcca gagacatggt ggcctcgttg cggctcatgg
     2101 cgtagctcat gatggaagaa acagggtcga tcatgccatc ctctggagac ttt
//
LOCUS       DAOPOA010000423         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_55398_length_2152_cov_3.413925, whole genome shotgun sequence.
ACCESSION   DAOPOA010000423 DAOPOA010000000
VERSION     DAOPOA010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55398_length_2152_cov_3.413925"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PKJ17_08700"
     CDS             complement(<1..524)
                     /locus_tag="PKJ17_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOD76093.1"
                     /translation="MESLYTLIEMVVKNIVVVLVLMGCVAYTTLLERKLLGRLQVRLG
                     PNRVGPFGLLQPIADGVKSFFKEDIIPDEADKTLYVLAPGISLFAALSMFAVIPFGDT
                     VTFLGRQIKLVVADLDTGLLYLLALATLGEYGVVLGGWASGNKYGVLGALRAAAQMIS
                     YEVALGLAVIGTIIL"
     gene            complement(528..>2152)
                     /locus_tag="PKJ17_08705"
     CDS             complement(528..>2152)
                     /locus_tag="PKJ17_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD76094.1"
                     /translation="FGYDFANNEARLATPTLKQGAAASEATLAEAVEATASALAKLDK
                     EGKTIGFIVSPRATNEEAYMISQIAGTFSKTRFGTAGYYHTGRVLETLRRMGIPYPYE
                     YEAINDCDLVVVAGADLLGNNHLLANKVREVVKNKGTKVAVVDPSSAALTRACDVWVK
                     VAPGSDALFFNGVASQLIADKKNDPEAGSLEGFSGSTGGGKGDAVRSGADEKSVDRFY
                     ALFAAASRVAVIFGTGISDRTESMEALLNLCLVKGVQKTGVVMPTALQSNAVGVISVL
                     PDAVSSGDVLGEADGLFIYEDDPFHYLGGKRVEESLKNKAFVAVCDMFPTGASALAHV
                     VVPSGSFAQKDGSYVAEDGYVRDVARAEGGSSPGFDYLRLLLDRLGGGLYRDEQEASR
                     VLFGKEVLVADDSGRAMLKPTNGQARLASAGKAAEGKPARPLTLVLRNVFFHHHLAGQ
                     GVYSKMVYLQNPAVAGDKLFISPEDAAALGIADGGQVIVESDHGTLQHPVTVKEGLGK
                     GVLEYRMLKNRQDVLKLADGYGKHIAVTVKKG"
BASE COUNT          417 a          697 c          613 g          425 t
ORIGIN      
        1 aggattatgg tgccgatgac cgcaaggcca agggccacct cgtagctgat catctgcgcc
       61 gcggcacgca atgccccgag caccccgtac ttgttccctg aggcccaccc gccgagaacc
      121 acaccatact caccgagggt cgcgagggca agcaggtaca gaaggcccgt atccaggtcg
      181 gcaacgacca gcttgatctg tctgcccagg aaggttacgg tgtctccgaa gggaatgacc
      241 gcgaacatgg agagcgccgc gaagagggag atgcccggtg cgaggacgta gagagtcttg
      301 tccgcctcgt caggtatgat gtcttccttg aagaaagact tgacgccgtc cgcgatcggc
      361 tgaaggagac cgaagggccc caccctgttg ggaccgagcc tgacctgcag acggccgagc
      421 agttttcgct caaggagcgt ggtataggcc acacatccca tgaggacgag caccacaacg
      481 atgttcttga ccaccatttc aatcaaagta tagagcgatt ccatgcctta accttttttt
      541 accgtcactg cgatgtgttt gccgtatccg tcggcaagct tcaatacgtc ctgcctgttc
      601 ttcagcatcc tgtattcaag cacgcccttg ccaagacctt ccttgaccgt gacggggtgc
      661 tgcagtgtcc cgtggtccga ttcgacgatg acctgaccgc cgtcggctat acccaacgcc
      721 gcggcgtctt ccggtgaaat gaacagcttg tctccggcaa ccgcgggatt ctggaggtac
      781 accatcttcg agtagacacc ctgtcccgcc aggtgatggt ggaagaagac attcctcagg
      841 acaagggtga gcggtctcgc cggcttgccc tccgcggctt tgcccgctga ggccagacgt
      901 gcctggccgt tcgtgggttt gagcatcgcc ctgccgctgt cgtccgccac gaggacctcc
      961 ttcccgaaga gaaccctgga tgcctcctgt tcgtccctgt acaggccgcc gcccaaccgg
     1021 tcgaggagga gcctcaggta gtcgaacccc ggcgaggatc cgccttccgc ccgtgccaca
     1081 tcgcgcacgt aaccatcttc cgcgacgtag ctgccgtcct tctgggcaaa gctgccggag
     1141 gggacgacga catgcgccag cgcggaggcg cccgtcggga acatgtcaca gaccgccaca
     1201 aaggccttgt tcttcaatga ttcttccacc ctctttcctc cgaggtaatg gaaaggatcg
     1261 tcctcataga tgaagaggcc atccgcctca cccagaacat ccccggaaga gacggcatcg
     1321 ggcaggaccg agatgactcc gacggcattg gactggagcg ccgtcggcat cacgaccccc
     1381 gtcttttgaa cgcctttcac gagacagagg ttgaggagcg cttccatgct ctccgtcctg
     1441 tcgctgatac cggtgccgaa tatgacagcc acgcgggagg ccgcggcgaa aagagcatag
     1501 aacctgtcca cgctcttctc gtcggccccg ctccgtactg catccccttt gcctccaccg
     1561 gtactacccg aaaaaccttc cagacttccc gcctccgggt cgttcttctt gtccgcgatc
     1621 aactgcgagg cgacgccgtt gaagaacagc gcgtcggaac ccggagcgac cttcacccac
     1681 acgtcacagg ctctcgtcag tgccgcggag gaagggtcga caacggccac cttcgttccc
     1741 ttgttcttca ccacttcccg cactttgttc gcaaggagat ggttattgcc gagaaggtcg
     1801 gcgccggcaa cgaccacgag atcgcagtcg ttgatcgctt catattcgta aggataggga
     1861 attcccatgc ggcgcagtgt ttcgagcacc ctgcccgtat ggtagtaccc ggcggtcccg
     1921 aaccgggtct tcgagaacgt gcccgcgatc tgcgagatca tgtaggcttc ctcgttcgtc
     1981 gcccgggggg acacgataaa accgatcgtc tttccttcct tgtcgagctt cgcgagagct
     2041 gacgcggtcg cctcgacggc ctcggcaagg gtggcttccg acgctgccgc gccctgcttg
     2101 agggtcggtg tcgcaagccg cgcctcgttg ttcgcgaagt cgtacccgaa gc
//
LOCUS       DAOPOA010000424         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_55474_length_2150_cov_3.177088, whole genome shotgun sequence.
ACCESSION   DAOPOA010000424 DAOPOA010000000
VERSION     DAOPOA010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55474_length_2150_cov_3.177088"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /gene="argH"
                     /locus_tag="PKJ17_08710"
     CDS             <1..896
                     /gene="argH"
                     /locus_tag="PKJ17_08710"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOD76095.1"
                     /translation="VVPFSHYLLGHYHALKRDRVRLTGALSMADVLPLGSGALAGSTI
                     PLDREWVKGRLGFSRVSENSMDTVADRDFVVDAIYAASMIMVHLSRLSEDLILFATQE
                     FGFISLPDELCTGSSLMPHKKNPDALELVRGKTGRVLSDLFGILTILKGLPFTYNRDL
                     QEDKEPLFHAVSTVKDALSIMALCVGGLKVRKENMERAAEESFMPAVEMAEYLTMKGM
                     PFREAHGVAGAAVRRCEDEGRLLGDMPLRELRKLSPLFERDVFDYIKPRSALRMRKST
                     GSASFGEVMKVIDAEKKYLGL"
     gene            868..1890
                     /locus_tag="PKJ17_08715"
     CDS             868..1890
                     /locus_tag="PKJ17_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76096.1"
                     /translation="MLKRNISVCSVILAALCLALAFSSCGKKGNPVPIPVVRAGTIND
                     LKGDVRDGVLFLSFTPPASLGRKETKAGEEEPKIASFKVVKGCGTCLGDLGPLRTIVL
                     SEKRGYTLAGNRLYFYDDDLVPGDSYAYRVYPVTEKGTTGEASNPFVIQWKEPPVPPE
                     GPLKIVEGDARVELSWTQEKEYLYNIYRYDDGRYPVVPLNPRPISTGLYMDAGLTNGR
                     TYTYEVRKVVPGPLPLEGEGLKATATPRDRTPPGAPTGVKAERKGKAIALTWQANAEK
                     DLGGYNVYRIMGSQAVKLNGVPLKETRYLDSGAPDFRFIAYHVTAVDTEGNESEPSQE
                     SIIEKE"
     gene            1895..>2150
                     /locus_tag="PKJ17_08720"
     CDS             1895..>2150
                     /locus_tag="PKJ17_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76097.1"
                     /translation="MDYFEYRNGELFCEDVPVKAVAGKVGTPFYLYSYGTFERHFRVF
                     DGAFGDMPHIVCYSLKANPNSALLRTVARLGGGADIVSGGE"
BASE COUNT          444 a          629 c          689 g          388 t
ORIGIN      
        1 aggtcgtgcc tttttcccac tatcttctcg gccactatca cgccctgaaa cgcgacaggg
       61 tgaggctgac aggggcgctt tcgatggccg acgtcctgcc cctcggcagc ggcgccctcg
      121 ccgggtcgac gatacctctc gacagggagt gggtgaaggg gagactgggg ttctcccgcg
      181 tgtcggagaa cagcatggac actgtcgccg acagggactt cgtcgtcgat gccatctacg
      241 cggcctcgat gatcatggtg cacttaagca ggctttccga ggacctcatc ctcttcgcga
      301 cgcaggaatt cgggttcata tcactgcccg acgagctttg caccggttcg agcctcatgc
      361 cccacaagaa gaaccccgac gcgctggagc tcgtccgcgg caagacgggc agggtcctct
      421 cggacctctt cggcatactc accatactga agggactccc cttcacatac aaccgcgacc
      481 tccaggagga caaggagccg ctgttccatg ccgtgagcac ggtgaaggat gccctttcga
      541 tcatggcgct ctgtgtcgga ggtttgaagg tgaggaagga gaacatggag agggcggccg
      601 aggagagctt catgcccgcc gtggagatgg ccgaatacct caccatgaaa ggcatgccct
      661 tccgggaggc ccacggtgtt gccggggcgg ccgtccgccg gtgcgaggac gaaggcaggc
      721 tccttgggga catgccgctc agggagctca ggaaactgtc gcccctcttc gagcgggacg
      781 tctttgacta cataaagccg cgcagcgccc tgcgcatgag aaagtccacg ggaagcgcct
      841 cctttgggga ggtgatgaag gttatcgatg ctgaaaagaa atatctcggt ctgtagcgtc
      901 atcttggccg cgctctgtct cgcgctcgct ttctcttcct gcgggaagaa agggaacccc
      961 gtgcccatcc ccgtggtgag ggcgggaacg ataaacgacc ttaaggggga cgtcagggac
     1021 ggcgttcttt ttctctcctt cacgcccccg gcgagcctcg gccgcaaaga gacgaaggcc
     1081 ggcgaggagg aaccgaagat agcgagcttc aaggtcgtca agggatgcgg cacatgcctt
     1141 ggggaccttg ggccattgcg cacgatcgtc ctcagcgaga agagagggta taccctggcg
     1201 gggaaccgtc tctatttcta cgacgacgac ctcgtgcccg gcgacagcta tgcctacagg
     1261 gtctaccccg tcacggaaaa aggaaccacc ggcgaggcgt cgaacccttt tgtcatacaa
     1321 tggaaggaac cccccgtccc ccccgagggc cccctcaaga tcgtggaggg cgacgccagg
     1381 gtggagctgt cctggacgca ggagaaagag tacctgtaca acatataccg ttacgacgat
     1441 ggcaggtacc ccgtggtgcc tctgaatccg cgcccgatat cgacggggct ttacatggac
     1501 gcggggttga cgaacggccg gacgtacacc tatgaggtta gaaaggtcgt tccaggtccc
     1561 ctcccgctgg agggagaggg cttgaaggcg accgccacac cgagagacag gacgcctccg
     1621 ggagccccga cgggcgttaa ggccgaaagg aaggggaagg cgatcgccct gacctggcag
     1681 gcgaacgcgg agaaggacct cggcgggtac aacgtttacc ggatcatggg ttcgcaggcg
     1741 gtgaagctca acggagtccc cctgaaggag accaggtacc tcgatagcgg cgccccggac
     1801 tttcgtttca tcgcctacca tgtcaccgcc gtcgataccg aggggaatga gagcgagccg
     1861 tcgcaggaat cgatcatcga gaaagagtga ggccatggac tattttgagt atcgcaatgg
     1921 agaactgttt tgtgaggatg tccccgtgaa ggcggtggcc ggaaaggtgg ggaccccttt
     1981 ctacctctat agctacggga cctttgaaag gcatttccgg gtcttcgacg gggccttcgg
     2041 agacatgccc cacattgtct gctattccct caaggcgaac ccgaacagtg ccctcttgag
     2101 gaccgtcgcc aggctgggag gcggtgccga catagtgtcc ggcggtgagc
//
LOCUS       DAOPOA010000425         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_55541_length_2148_cov_4.667941, whole genome shotgun sequence.
ACCESSION   DAOPOA010000425 DAOPOA010000000
VERSION     DAOPOA010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55541_length_2148_cov_4.667941"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKJ17_08725"
     CDS             complement(<1..252)
                     /locus_tag="PKJ17_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002867082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HOD76098.1"
                     /translation="MFDLNCDVGESFGAWVMGNDAEIIRHVTSANIACGVHASDPSVM
                     RRTVELCRQYGVMAGAHPGYPDLQGFGRRHMDMQGGEVAD"
     gene            510..914
                     /locus_tag="PKJ17_08730"
     CDS             510..914
                     /locus_tag="PKJ17_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76099.1"
                     /translation="MAKKGMETAKRMSRVAASDSDRTRLRRFLFLWAPIIVIVLVGFY
                     VVALDPAKPTGRTIEASVIGKEEAAGGPSSTSMFRVRFDNGEEAVMFIPERQVPPPPG
                     RILAEEYTTSLLSKRTYRYLRAVGATGTPGTP"
     gene            complement(930..1577)
                     /locus_tag="PKJ17_08735"
     CDS             complement(930..1577)
                     /locus_tag="PKJ17_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76100.1"
                     /translation="MNLLSILAFAVSSSFDNVGVGVAYGMRGVCIPFLSNLVIAAMNG
                     SGTLVSMLAGTSATRFLESGVSSVLGGSIMICTGLWVFIDWMRHPRRPGSIEEDMVER
                     SRSLAGKSFPDMVWAIFRNPFITHPQCSGSMKAVETLLIGSGLTMTNLVTGLAAGMLG
                     VDVAGLTVASFLSNLVAMQLGTSAGASRALRRLGHASGAISGVLLAGIGLAEMVF"
     gene            complement(1574..>2148)
                     /locus_tag="PKJ17_08740"
     CDS             complement(1574..>2148)
                     /locus_tag="PKJ17_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOD76101.1"
                     /translation="WAEEVPDGFIFAMKAPRVITHVKGLKDVEEEMDHLFRMLSVLGA
                     RLGPVLFQFPATFHVDPAVLEDHVDLIPPGLSCAFEFRSPSGLHPRIPDILAGSGCTL
                     CISDSDENPVEMIPQVPWGYLRLRRGAYSEAGLSRWLEKIGSQDWNRAFVYFKHGEGA
                     GSPGAAMRFDELVRNDRRPAVAGGTPGDGT"
BASE COUNT          456 a          689 c          625 g          378 t
ORIGIN      
        1 atccgcgacc tcgccgccct gcatatccat gtggcgcctg ccgaacccct ggaggtcggg
       61 ataaccgggg tgtgccccgg ccatgacacc gtattgcctg caaagctcca cagtccttct
      121 catcactgac gggtccgacg cgtgaacacc acaggcaatg ttggcggagg tcacatggcg
      181 tatgatctcc gcatcgttcc ccatcaccca cgctccgaaa gactccccca catcgcaatt
      241 cagatcgaac acgccacgca tagttcccaa ttctaccaga aaaccgcctg caaagacagt
      301 ccccgctctc gtttgttcgg tgccccgatc gtaggctggg agcggtgcct cccgcttcag
      361 cgggaggcaa aactggccgg attttggaag gctccgaaat ccggccgcat cttttctctg
      421 cgatctccgc ggtctcaagc taagcgggcg ggaggcaggg tcttttcagc ctttacaaca
      481 cttcctgctt ctgctaagtt ttgggagcca tggcgaaaaa gggtatggaa acggcaaagc
      541 ggatgagcag ggtcgcggct tccgatagtg acaggacccg gcttaggagg ttccttttcc
      601 tctgggcgcc catcatcgtg atcgtccttg tcggcttcta cgtggtcgcc ctcgatccgg
      661 caaagccgac gggcaggacc atcgaagcga gcgtcatcgg gaaggaggag gctgccggcg
      721 gaccgtcatc gacctcaatg ttcagggtcc gtttcgataa tggggaggag gcggtcatgt
      781 tcatccccga acgacaggtt ccaccgccgc cgggacggat cctggcggag gaatacacca
      841 caagccttct cagcaaaagg acctacaggt acctgcgtgc cgtaggcgcc acggggaccc
      901 cgggaactcc atagccacga gccctcggtt cagaagacca tctccgccaa ccctatgccg
      961 gcaaggagca caccggatat ggcaccgctc gcatgtccca gtcggcgtaa cgcgcgcgaa
     1021 gcgcccgcgc tcgtcccgag ttgcattgcc acgaggttgg agaggaagga ggcgacggta
     1081 aggccggcga cgtcgacgcc cagcatgccg gcggccagac ctgtcacaag gttcgtcatc
     1141 gttagacccg agccgatgag aagcgtctca accgccttca tcgatccgct gcattgcggg
     1201 tgggtgataa atggattcct gaagatggcc cacaccatgt ccggaaagct cttgcccgcg
     1261 agggagcggg acctctcgac catgtcctct tcgatggaac ccggccggcg gggatgacgc
     1321 atccagtcga tgaataccca gagacccgtg catatcatta tggatccccc gaggacgctt
     1381 gaaaccccgg actcgaggaa ccgggtcgcc gacgtgcccg caagcatgga aacgagggta
     1441 cccgatccgt tcatggctgc tattacaagg tttgataaga aggggatgca gacccctctc
     1501 atcccgtacg caacacccac acccacgtta tcgaaactgc tggagaccgc gaacgccagg
     1561 atggacagca ggttcatgtc ccatcgcccg gtgtgccacc ggcgactgcc ggccgcctgt
     1621 cgttgcgcac gagttcatcg aaccgcatcg ccgctcccgg gctccctgca ccctcgccat
     1681 gcttgaaata gacaaacgcc ctgttccaat cctgggagcc tatcttctca agccaccgcg
     1741 acaacccagc ctcactgtag gcgccgcggc gaagacgcag atatccccag ggcacctgtg
     1801 ggatcatctc cacgggattc tcatcggaat cggaaatgca gagggtacat ccggagccgg
     1861 cgaggatgtc cggtatccgc gggtgaagtc ccgacggact ccgaaactcg aaggcacagg
     1921 aaagcccggg agggatgagg tccacatggt cctcgaggac tgcgggatcg acgtggaagg
     1981 tcgccgggaa ctggaacaga acggggccga ggcgagcgcc cagcaccgag agcatcctga
     2041 aaaggtggtc catctcctcc tccacatcct taagcccctt cacgtgggtt atcacccgcg
     2101 gcgccttcat ggcgaagata aacccatcgg ggacctcttc cgcccagg
//
LOCUS       DAOPOA010000426         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_55554_length_2148_cov_2.962733, whole genome shotgun sequence.
ACCESSION   DAOPOA010000426 DAOPOA010000000
VERSION     DAOPOA010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55554_length_2148_cov_2.962733"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKJ17_08745"
     CDS             <1..279
                     /locus_tag="PKJ17_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOD76102.1"
                     /translation="AAIPGILTGIMLSIGRAVGETAAIIFTMGSSLRLPLSLMDSGRT
                     MAVHFYILAREGISMEKAYGTAVVLVLSILLINILAYYIMNKAIARYS"
     gene            287..1042
                     /locus_tag="PKJ17_08750"
     CDS             287..1042
                     /locus_tag="PKJ17_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOD76103.1"
                     /translation="MDTKIATDRLRLSFGTTEVLKSVTIKVHRNTITGFMGPSGSGKS
                     TLISVINRMIDFENNVKIEGDATIDGTSILDGSINPIELRRRVGTVFAVPIPLPRSIF
                     ENIAYGPRLKGTSDRTTLHHIVEDSLKKAFLWDEVKDRLGTSALKLSGGQQQRLCLAR
                     TLALKPEIILLDEPCSGLDPISTAKIEDALSELKSEYTIILVSNNTKQIARISDFSAF
                     FYLGELIEYDTTEKVFTAPAEAKTEDYIQGKFG"
     gene            1042..1794
                     /gene="pstB"
                     /locus_tag="PKJ17_08755"
     CDS             1042..1794
                     /gene="pstB"
                     /locus_tag="PKJ17_08755"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOD76104.1"
                     /translation="MDRFALRTNKLNLYYGDFHALKNVDFQVHSKAITALIGPSGCGK
                     STLLRCFNRMNDLLEEVRIEGEIYVHNEKPQDIDIINLRKKVGMVFQRPNPFPFTVYE
                     NMVYGLKVHGIGHRRGWREIVDRCLHSVGLLDELKDKLHTPALDLSEEIKQRLCVARL
                     LTVEPEIILLDEPCSALDPIATMRIEELMIELKERYTILIVTHNMQQAARVSDYTGFM
                     LLGDLVEFGETSHIFTSPRDERTEGYITGRYG"
BASE COUNT          518 a          652 c          555 g          423 t
ORIGIN      
        1 gcggcgatcc ccgggatact taccggcatc atgctcagca tcgggcgggc cgtgggcgaa
       61 acggcggcaa tcatcttcac aatgggcagt tccctgaggc ttcccctgtc cctcatggat
      121 tcgggcagga ccatggcagt ccacttctat attctggccc gggaaggcat ttccatggag
      181 aaggcgtacg gcacggccgt tgtcctcgtg ctcagcatcc ttctcatcaa catactcgcg
      241 tactacatca tgaacaaggc cattgcgcgg tattcatagg gaagaaatgg acacaaagat
      301 cgcgactgac agattacgat tatccttcgg cacgacagag gtgctcaaaa gcgtcaccat
      361 caaggttcac aggaacacca tcaccggttt catggggccc tcgggtagcg gcaaatcaac
      421 cctcatctcc gtcatcaacc gcatgataga cttcgagaac aacgtgaaga tcgagggcga
      481 tgccaccata gacggaacga gcatccttga cgggagcatc aaccccattg agctgcgaag
      541 acgtgtagga accgttttcg ccgttcccat ccccctgccc cgttccatat tcgagaacat
      601 cgcctatggg cccagactta aagggacgag cgaccgcacc accctgcacc acatcgtaga
      661 ggacagcctc aagaaggcat tcctctggga tgaggtgaag gacaggctcg gcacgtcggc
      721 gctcaagctc tccggcggac agcagcaaag gctctgcctc gcgcggaccc tggcgcttaa
      781 accggagatc atccttctcg atgagccttg ctccggcctc gaccccatct caacggcaaa
      841 gatcgaggac gccctgagtg agctgaagtc ggagtacacc atcatcctcg tgtccaacaa
      901 cacgaaacag atcgcccgga tcagcgactt ttcggctttc ttctacctcg gggagctcat
      961 agagtatgat acgacggaaa aggtctttac cgcccccgcc gaggccaaga ccgaagacta
     1021 catacaggga aagtttggat aatggataga ttcgccctga gaacgaacaa gctcaacctc
     1081 tattacgggg attttcacgc cctcaagaac gtcgatttcc aggtccactc caaggccata
     1141 acggccctca tcggcccgtc gggatgcgga aagtcaacgc ttcttcgctg tttcaaccgt
     1201 atgaacgatc ttctggagga ggtccgcatc gagggcgaga tatacgtcca caacgagaag
     1261 ccgcaggata tcgatatcat caacctgagg aagaaagtgg gcatggtctt tcagcgccca
     1321 aaccccttcc ccttcactgt ttacgagaac atggtctacg gcctcaaggt ccacggaata
     1381 ggacacaggc gcggatggag agagatcgtc gatcgctgtc ttcactccgt cggccttctc
     1441 gacgagctca aggacaaact ccacaccccc gccctcgatc tctcggaaga gataaagcag
     1501 cgtctttgcg tcgcccgcct cctgacggtg gaaccggaga taatcctcct cgacgagccc
     1561 tgctccgccc tagaccccat cgccaccatg aggatagaag agctcatgat cgaactgaag
     1621 gagcgctaca ccatcctcat cgtcacccac aacatgcagc aggccgcccg agtctccgac
     1681 tacacgggct tcatgctcct cggcgacctc gtcgaattcg gcgaaacatc ccacatcttc
     1741 acctcccccc gggacgaaag gactgagggc tacatcacgg gaagatacgg gtagcagcgt
     1801 cgctttgcga cgcatggacg ggcacacgag tctgcgcttc gcgcaaacgg gcaaacgcgc
     1861 taaaagcaac taagaaacag tgtcttttca gtgtccggtt ttccccgtct gcccgtgtgc
     1921 ccgtttttcg cttaagcgaa agacccgtgt gcgtcttttc gctcctgtgc tctccgtcat
     1981 tgacttcctt cttttcccat agtaagtttt cgaaaaaaaa acacgcaggg ggatggtatg
     2041 gggaactttc tggaagtact cgaatggttc gatgatacag ggaaggagat agcccacagg
     2101 ctgcccgagg aagggtcggg cgacatcaag tacggcgccc agctcacc
//
LOCUS       DAOPOA010000427         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_55564_length_2148_cov_2.418538, whole genome shotgun sequence.
ACCESSION   DAOPOA010000427 DAOPOA010000000
VERSION     DAOPOA010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55564_length_2148_cov_2.418538"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..647)
                     /locus_tag="PKJ17_08760"
     CDS             complement(213..647)
                     /locus_tag="PKJ17_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76105.1"
                     /translation="MKKVLVAALVVAMGLSLGGMAYAAWGGGPGPGYGTQVDTKALRN
                     FQKETLPLRDELIAKRAEIQTEYAKENPDWNRIATLQKEMVDLRTKIQSSARKQGLSD
                     RGYGPGYGRGFGGGYGGGWMMGRGGHGPGHRGGYGGGYCNTW"
     gene            complement(720..2087)
                     /locus_tag="PKJ17_08765"
     CDS             complement(720..2087)
                     /locus_tag="PKJ17_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD76106.1"
                     /translation="MLRSLQLKFLLLLLAVVAIALVSTILFRHFMLRDFNAYLEGEAE
                     DKVYLIQADLEGSYERSGGWKTDIQAQEAIRALMSGFEMRLTDKEGKLVIDTGSAIES
                     ASPLVKRRLMALAQFNVPDGAGAFVPYPLFLAGKQIGTLQIRQLRPVREGVFLQRSNG
                     FLVLSIAVIGGIAVLMSILFSRRLTRPVKELAVAASAISRGDLQKRVAVSRRDEVGEL
                     AENFNFMAKALETQEALRRKLIGDVAHELRTPLSVMRGELEGLIDGLIPNDPARLQSL
                     YEETGRLGNMVDAIEELGKAEASLLSLNRQWVDVRPFLQNILERFRAQSQDRGVAMEL
                     TCPEGFRLHADPERLSQVILNILANALRATPGGGSVSIRVGPAEGGLEIVVTDTGRGI
                     RQEDIPFIFERFYHGPGGGLGIGLTIVKELVEAHGASIAAASMPGEGASFRILFPAEA
                     VHNSS"
BASE COUNT          439 a          693 c          564 g          452 t
ORIGIN      
        1 cgccataagg tccccattgc atacccgggc aataatggcc gtaaggcgaa ccccgataca
       61 tgcctcttcc catgggttgc gccaccgaga ccacaagcag cgtcaatatc acaagaaaaa
      121 aaacggtcag gtatgcaatg cgcttcaaca tgccatctcc ttcgggagca aggctgcggc
      181 ttcgttccgc aaccttgctc ccggatcagt gcttaccatg tgttgcagta accgccgccg
      241 taaccacctc tgtggccggg accgtgaccg ccgcggccca tcatccagcc gccgccgtaa
      301 ccgccgccga aaccgcgccc gtaacctggt ccgtaacccc tgtcagagag gccctgcttc
      361 ctggccgagc tctgtatctt tgtcctcaga tcgaccatct ctttctgcag ggtcgctatc
      421 cggttccagt ccggattctc cttggcatac tcggtctgga tctccgccct ctttgcgatg
      481 agttcatccc ttaacggcag ggtctccttc tggaagttcc tcagggcctt ggtgtccacc
      541 tgagtaccgt accccggtcc cgggcctcct ccccaggctg catacgccat accgccgagg
      601 gaaagtccca ttgccacaac caatgctgct accagtacct tcttcatctc aaacctcctt
      661 gtgtgtatga atttcatgta cacagtataa ggaaggactg tgaagaggta aggaagacat
      721 tatgaagaat tgtgaaccgc ctccgccggg aaaaggatcc tgaaagatgc accttctccc
      781 ggcatgctcg ccgccgctat gctcgcacca tgggcctcca ccagttcttt cactatggta
      841 aggccgatcc cgagacctcc tcccgggccg tgatagaatc tctcaaagat aaagggaata
      901 tcctcctgcc tgatccccct gccggtatcc gtgacgacga tctccaatcc gccttccgcc
      961 ggtcccaccc ggatcgaaac gctcccacca ccgggggtgg ccctgagggc gttggccaga
     1021 atgttcagga tgacctggct caaccgttcg gggtccgcgt ggagcctgaa cccttctgga
     1081 cacgtaagtt ccatggccac acccctgtcc tgggactgcg ccctgaacct ctccaggatg
     1141 ttctgaagga agggccgcac gtccacccat tgccgattga gcgagagcag gctcgcctcc
     1201 gccttgccca gctcctcgat ggcgtcgacc atgttcccga gtcttcccgt ttcctcatag
     1261 agagactgga gacgggcggg atcgttgggt atcaggccgt ctatcaaacc ctccagttct
     1321 ccgcgcatga cactgagcgg ggtccgcaac tcgtgcgcca cgtcgccgat cagtttcctc
     1381 ctcaacgctt cctgggtctc gagggccttt gccatgaagt tgaagttctc cgcaagttcg
     1441 ccgacctcgt cgcgtcttga gacggccacc cgcttctgca aatcgccccg actgatggcg
     1501 gatgccgcca ccgccagttc ctttacggga cgggtaaggc gacgggagaa gagaatactc
     1561 ataagtactg cgatcccgcc gatgacagcg atcgagagca ccaggaaccc attggatctt
     1621 tggagaaaca cgccctccct tacgggacgc aattgcctga tctgaagggt cccgatctgc
     1681 tttcctgcaa ggaacagggg ataaggaacg aatgccccgg cgccgtcggg cacattgaac
     1741 tgcgccaggg ccatcaatct ccttttgacg aggggagagg cgctctcgat ggcgctgccc
     1801 gtgtcgatca cgagctttcc ctccttatcg gtaagcctca tctcgaaacc ggacatcaag
     1861 gcccgtatgg cctcctgggc ctgaatatcg gtcttccatc ccccgctgcg ctcataggag
     1921 ccttccagat cggcctgtat caggtagacc ttgtcctcgg cctcgccttc caggtaggcg
     1981 ttgaaatccc ggagcatgaa atgcctgaag aggatggtgc tgacaagggc aatggctacg
     2041 acggcaagga gaagcaagag gaacttgagc tggaggctcc taagcatcct tccttcccgt
     2101 gaaccggtat ccaacgccat aaatggtgtg gatgaagagc ggattctg
//
LOCUS       DAOPOA010000428         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_55797_length_2141_cov_2.168744, whole genome shotgun sequence.
ACCESSION   DAOPOA010000428 DAOPOA010000000
VERSION     DAOPOA010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55797_length_2141_cov_2.168744"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="PKJ17_08770"
     CDS             complement(<1..141)
                     /locus_tag="PKJ17_08770"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOD76107.1"
                     /translation="MVDQEGLVIIFTGDGKGKTSAALGIALRALGRGQRTAIFQFMKG
                     RER"
     gene            complement(318..1700)
                     /locus_tag="PKJ17_08775"
                     /pseudo
     CDS             complement(318..1700)
                     /locus_tag="PKJ17_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(1894..>2141)
                     /locus_tag="PKJ17_08780"
     CDS             complement(1894..>2141)
                     /locus_tag="PKJ17_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOD76108.1"
                     /translation="GNGELLINFLNELLFVWDVERFIPAEVAVSFEREGLTALLRGEP
                     FDEGRHSIELELKAVTYHKFAITEEKGKFKAIIIIDV"
BASE COUNT          428 a          672 c          570 g          471 t
ORIGIN      
        1 tctctcccgg cctttcataa actggaagat ggccgtcctt tgcccccggc cgagggcgcg
       61 aagggctata cccagtgcag ccgaggtctt ccccttccca tcgccggtaa agataatgac
      121 aagcccctct tgatccacca taccagttaa taccattatt gtaaggaccg ttgcaaccgc
      181 ttgaacagct tgaaccgttt gaacgcaaga aaacaaacag aaagctcata ccgtcaaagg
      241 gatgcgatct tttttcggaa atagcgagac cttgaacgtt caagcggttt aagcggctca
      301 agcggttaaa accatcttta cccctttatg gaccccatcg ggacgatctt tgccaccagc
      361 cgggagatcc ccgccctgtg gaccacctgg acgacctttg atacgtcctt gtaggcctcg
      421 ggcatctctt ccgcgagcgt ccccttactg gcggccctca catagacacc gcgctcttcc
      481 atttctctgc agatcgatcg cccccgggcc gatctcagcg cctggttcct gctcatgatc
      541 ctgcccgcgc catggcaggt gcttccaaag gtctcgtcca tggcgcgctg tgtccccgcg
      601 aggacgtagg aggccctgcc catatcaccg ggaacgagaa cgggctgccc cacctccctg
      661 tatctgttgg ggacgagacc gtggtgcggg gagaaggccc tcgtggcacc tttccggtgg
      721 acgcagagag tcatctcctt gccttccacc cggtgcgttt ccttcttggc tatgttgtgg
      781 catacgtcat agacgagtcc gaggccaagc gaagacgcgc ttgcggcgaa cacgttctcg
      841 aaggtctcac ggacccagtg cgttatgagc tgacggttcg cgaaggcata gttggcggcc
      901 gcggccatgg ccgagaaata gcgctgtcct tccggagatt taaggggggc gcagcacagc
      961 tgcctgtcga cgagatctat gccgtacttc gcggcggcgg acagcatgtg tcgtatcgac
     1021 tcgtcacaga cctggtagcc gagaccgcgc gagcctgtgt ggatcataat ggtggcctgg
     1081 tcgcgaaaga gtccgaaagc ctgcgccgcc tcctcatcga atacctccgc gacatagcct
     1141 gcctcgacga aatggttccc gctgcctacg gttccgagct ggtcttttcc ccgttcgtac
     1201 gccttgtcgg aaacggcgtc cggatcggct ccatcgagac agccctgatc ctcgatgttc
     1261 tcgagatcgg actgggtgcc gtaaccgttc gctattgccc atcgcgcccc tttcccgagc
     1321 actcccctca gttcgccgcg agagagcttc aagtccttgc ggcgggagcc gatgccgctc
     1381 gggacatttc gaaagaggcc ctctatgatg tccggcaacc tgtccttcac atcattcgcg
     1441 ctcagcacgg agcgcaggag gcgaacccct cagttgatat cgtagccgac gcccccggga
     1501 gagatgatcc cttcatcgac gtcgaaagcc gccaccccgc ctatgggaaa gccgtaaccc
     1561 cagtggatat cgggcacagc aagggacgcc ttgacgatcc cgggaagcgt ggctacattc
     1621 cggacctgtt ctatactttc gtcggtgcca agatatccga gcaactcctc attgatgtag
     1681 atgatgccgt caacccgcat cgccccggat ctcctgacga gataacggtt ctcatcaagt
     1741 ttctcgagcg tccttttttc ttcgctcata tcttacctcc cgagaagaag gttccacgtt
     1801 cgggactgac cagcgcagaa caggattctt tggcctttga ctcgaaacgc gcaacccgga
     1861 acccgcaacc gtctcttgcc tgaaaccggt ctcttacacg tctataataa tgatagcctt
     1921 gaatttcccc ttctcttcgg ttatggcaaa cttgtggtag gtgacggcct tcaattccag
     1981 ttcgatgctg tgcctgccct cgtcgaaggg ctcccccctc aaaagggcgg tgagaccttc
     2041 tctttcaaag gagacggcca cttcggcggg gatgaacctt tccacgtccc agacaaagag
     2101 gagctcgttg aggaaattga tgaggagctc cccgttgccc g
//
LOCUS       DAOPOA010000429         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_56027_length_2134_cov_4.553632, whole genome shotgun sequence.
ACCESSION   DAOPOA010000429 DAOPOA010000000
VERSION     DAOPOA010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56027_length_2134_cov_4.553632"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1306)
                     /gene="fbp"
                     /locus_tag="PKJ17_08785"
     CDS             complement(206..1306)
                     /gene="fbp"
                     /locus_tag="PKJ17_08785"
                     /EC_number="3.1.3.11"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF014057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphate aldolase/phosphatase"
                     /protein_id="HOD76109.1"
                     /translation="MKTTLSIIKADIGSIGGHIMPSKKLIKRVIEFVESEAKGLVNDF
                     FVSHTGDDVAILFAHTNGVGFEKLHKLAWDAFLAGTQVAKKQGLYGAGQDLLKDSFSG
                     NVKGMGPAVAEMEFEERPNEPFVMFAADKTDPGAYNLPLYLMFCDPMYNSGLILSPNM
                     AKGFTFVIMDVEHTEGDKVIELNAPEELYDIAALLRDNERYVVESVYSRETGEIAASA
                     CTSRLHNIAGKYTGKDDPVMLVRCQGPFPATGEVLAPFNIGHYVAGFMRGSHTGPLMP
                     VKMNSSVSYFDGPPVVAAMGFCVHEGKLTEAADCFDHPYWDYIRNNVSRKATEIRQQG
                     FSGAAMLPYSELEYGGIVKKMEKLEPKFKVRG"
BASE COUNT          425 a          588 c          624 g          497 t
ORIGIN      
        1 tcgatgagga cggaccccat cttctccata tcatggtcat tcatctgaca accgaaattt
       61 tcgataacaa atctcatatc aaaagctcgt gttaagtttt aagtgttaag ttttaagtga
      121 aagacaaaga ccatgtcaag ccttgagtgg ttgaggcctg acacggtctt tgcctaaaac
      181 ttaacaccta aaacttaaaa cgtttttatc ctcttacctt gaactttggt tccagttttt
      241 ccattttctt tacgatgccg ccgtactcaa gttcgctgta ggggagcatc gcggcgccgg
      301 agaagccctg ctgcctgatc tcggtggcct tccgcgaaac gttgttcctg atgtaatccc
      361 agtagggatg atcgaagcag tcggccgctt ccgtcagttt gccttcgtgg acacagaagc
      421 ccatggcggc gacgacgggc ggtccgtcga agtaggaaac gctggagttc atcttgacgg
      481 gcataagggg acccgtgtgg ctgccgcgca tgaagccggc gacgtagtgg ccgatgttga
      541 agggcgcgag cacctcgccc gtggcgggga aaggaccctg gcagcggacg agcatgacgg
      601 ggtcgtcctt gccggtatac ttgccggcta tgttgtggag ccgggaggtg caggcggaag
      661 cggcgatctc gccggtttcc cgggaataga ccgattccac gacgtaacgc tcgttgtccc
      721 tgagcagggc ggcgatatca tagagttcct cgggggcgtt caattcgatg accttgtcgc
      781 cctcggtgtg ctcgacgtcc atgatgacga acgtgaagcc tttcgccatg ttgggggaga
      841 ggatgagccc ggagttgtac atggggtcgc agaacatgag gtaaagaggc agattgtaag
      901 cgccggggtc cgtcttgtcc gccgcgaaca tgacgaaggg ttcgttcggt ctctcctcga
      961 actccatctc cgcgacggcc ggccccatgc ccttgacgtt ccccgaaaag gagtccttca
     1021 gaaggtcctg gcccgctccg tagagcccct gtttcttcgc cacctgggtc cccgcgagga
     1081 aggcgtccca ggcgagcttg tggagtttct cgaagccgac cccgttcgtg tgggcgaaga
     1141 ggatggcgac gtcatcgccg gtgtggctca cgaaaaagtc gtttacgaga cctttggctt
     1201 cggactctac gaattctatt acccgcttga tcagtttctt gctgggcatg atatgcccgc
     1261 cgatgctacc gatgtcagct ttgataatgg ataacgttgt cttcataaca acctcctgaa
     1321 gtgtttttga taaattttca tcgaaccggc gtctgttata tcgagaccca ggggggtgat
     1381 ggaaacgtag cccttctcta tggcgtagaa gtcggtaccg cgcctgcgtt cgtatctttc
     1441 accgttgccg cctatccagt agtatttccg gccccgtgga tcgactcttt cgtgaacaat
     1501 atcattatat attcttttgc cgagccttgt cacggaaaaa cctttgagct tctctcgcgg
     1561 aaggttggga atattgatgt tgacgacggt gccctgggga aagactcctt ccgtaagcct
     1621 ctccatgacg gcgcaggcga ctcccgccgc gtcgtcgaag aggaacccct cacgagcgca
     1681 gatggagacg gccatcgacg ggattcccag gaaggcccct tctctcgccg ccgccaccgt
     1741 tcccgagtag ttcacgtccc ggcccatgtt cggtcccgtg tttatgcccg agaccacgaa
     1801 atcagggcgc cgcgggagga cggcgtggac gccgaggagg acgcagtccg cgggcgttcc
     1861 gttcgtcgcg tacgcgctcc gcgtcacctc acggaccctc aagggtttgt gcagcgtcac
     1921 ggcgtgggcg acgcatgtcc gttcgcgctc cggcgcgatc atgtacacgt cgtgtttttc
     1981 cgacaggtat ctgccgaggg tgacgatccc ctttgaatgg atgccgtcgt cgttggtgag
     2041 aaggataagc aagaagtacc cggtctcgag aaataaaata tcggggcgac gggatttgaa
     2101 cctgcggcct cctgcacccc aagcaggtgc gcta
//
LOCUS       DAOPOA010000430         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_56234_length_2129_cov_3.192382, whole genome shotgun sequence.
ACCESSION   DAOPOA010000430 DAOPOA010000000
VERSION     DAOPOA010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56234_length_2129_cov_3.192382"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PKJ17_08790"
     CDS             complement(<1..896)
                     /locus_tag="PKJ17_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOD76110.1"
                     /translation="MGDIRKMLNPAVVALIGATEREGSIGKRILDNLLASEGRKVLPV
                     NPGRKTALGLDCAGTVSDIDGEIDLAVIAVPAASVPRVVDECGRRGVQGAIIVSSGFR
                     ETGEDGKTLEKDLLRAREKYGIRIVGPNCLGIMIPAIDLNATFLKIRPEPGNIAFLSQ
                     SGALGDAIMDWGTSNHVGFSMFASLGSMIDVDFADLIDYLSDDYATRSIMLYMERVGT
                     AKRFMSAARGFARTKPIVVLKPGRFEGSTRAVESHTGEAIGDDRVYGAAFKRAGIVRV
                     KEIADLFDTSRVLVSRTMPRGAR"
     gene            1009..1608
                     /locus_tag="PKJ17_08795"
     CDS             1009..1608
                     /locus_tag="PKJ17_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76111.1"
                     /translation="MLPLICEINSLWEPVYPHLARHIGEVYGRNAGAVLEVGPFCGVV
                     YDLVRQGIGSSFCIASFPRGMDSFYTGEIAERGMRDRVDMVRTSPDLGGIADNTIDLL
                     VFRGALFFPSLFKVDHRAILRVLRPGGTAFVGGGFGKYTPPEVIRPLADRSRELNYLI
                     GKKETTVDEIQKDLEAAGISENAEFITEGGLWVIIRKDA"
     gene            complement(1727..2020)
                     /locus_tag="PKJ17_08800"
     CDS             complement(1727..2020)
                     /locus_tag="PKJ17_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76112.1"
                     /translation="MKIRVKVDSNLRIKNVFEPPLELEMSQDENTLKDVLGKLSERYP
                     YLRFLEKGEMGDDLHHLYLNGESHFSFPEGLNRKISEGDTVRVDAYMDPLAGG"
BASE COUNT          463 a          651 c          554 g          461 t
ORIGIN      
        1 cgcgcacccc tgggcatcgt ccgcgacacc aggacccggg aggtgtcgaa caggtcggcg
       61 atctccttta cccgtactat cccggcacgt ttgaatgccg cgccatacac cctgtcgtct
      121 ccgatcgctt cgccggtgtg ggactcgacg gcccgcgtgc tcccctcaaa acgtccgggc
      181 ttgaggacga cgatcggctt cgtccgagcg aatccccgtg cggcactcat aaacctcttc
      241 gcggtgccga cgcgttccat gtaaagcatg atgcttcgag tcgcgtaatc atccgagaga
      301 taatcgatga ggtcggcaaa atcgacatcg atcatcgaac cgagagaagc gaacatgctg
      361 aatcccacgt ggttgcttgt cccccagtcc atgatcgcgt cgccaagcgc cccgctctgg
      421 gagagaaacg caatgttccc cggctcgggg cggatcttga ggaacgtggc attcagatcg
      481 atagcgggta tcatgatgcc caggcaattg gggcccacga tcctgatccc atacttctcc
      541 ctcgccctga gcaggtcctt ctccagggtc ttcccgtctt ctcctgtttc ccgaaaaccg
      601 gaagacacga tgatggcccc ctgcacaccc cttctgccgc actcgtccac gacccgcggc
      661 acacttgctg ccggcacggc gatgacggca aggtctatct ccccgtcgat gtcggacaca
      721 gtgcccgcgc agtccaatcc cagagcggtc ttcctgccgg ggttgaccgg caggaccttc
      781 cgtccctcag aggcaagcag attgtcgaga atgcgtttcc cgatggaccc ctccctctct
      841 gtggcaccta tcagggcaac gacggcagga ttcagcatct ttcttatatc acccatggtt
      901 ccattatcct cagcgatcga aagaaatgat agaataccac agagagcgcc atttgtcctg
      961 attttcaagg gatagacctt tccggtacct tttggtatac ttcacgacat gcttcccctc
     1021 atttgcgaga tcaattcact ctgggagccg gtttaccccc acctggcacg gcacatcggc
     1081 gaggtctacg gccgcaacgc gggagcggtc ctggaagtcg gccccttctg cggggttgtg
     1141 tacgatcttg tcaggcaggg aatcgggagc tccttttgta tagcctcttt tcctcgcggt
     1201 atggacagtt tttacacggg agagatagca gaacgaggaa tgcgggacag ggtcgacatg
     1261 gtccggacgt cgcccgacct gggcggcatc gcggacaaca ccatcgacct tctcgtcttc
     1321 agaggggcct tgttcttccc gtcactgttc aaggtcgacc accgggccat cctccgcgtc
     1381 ctcagacccg gcgggacggc cttcgtgggc ggaggcttcg gcaaatacac acccccggag
     1441 gttatccgcc ccctcgcgga ccgctcacgg gaactgaatt acctcatagg caagaaagag
     1501 accaccgtcg acgaaataca gaaggacctt gaagcggcag gcataagcga aaacgccgag
     1561 ttcatcactg aaggggggtt gtgggtaata atcagaaaag acgcttgaat cgcttgagag
     1621 ttgaaagact aaaagaacct ctttggaaac ggttgtaacc aatgatgctc ggattgcctg
     1681 taaccctcaa gccattcaag cgactcaagc tattcaagcc gtcttttcat ccccctgcca
     1741 gggggtccat gtaggcgtcc acgcggaccg tgtcgccttc gctgatcttt ctgttcagtc
     1801 cctcggggaa tgaaaaatgg ctttctccat tgagatacag gtggtgaagg tcatcgccca
     1861 tctccccttt ctccaggaac ctcaggtagg gataccgctc ggaaagtttg ccgagaacat
     1921 ccttcagggt gttctcgtcc tgactcatct ccagttcgag cgggggctca aaaacgttct
     1981 tgatccgaag gttcgaatca actttcaccc tgatcttcat cgtcgcacct ccatacagcc
     2041 aatacagtta ataatataca acattacgag gaaaaagcac aggtcccata ggacacatcc
     2101 gacccacagg acctgcgatg tcttttaac
//
LOCUS       DAOPOA010000431         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_56285_length_2128_cov_2.121563, whole genome shotgun sequence.
ACCESSION   DAOPOA010000431 DAOPOA010000000
VERSION     DAOPOA010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56285_length_2128_cov_2.121563"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKJ17_08805"
     CDS             <1..540
                     /locus_tag="PKJ17_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOD76113.1"
                     /translation="KLNTFAYLRIRGAMIDELRSRDWFPRSARSKSKKINEVVRKLEN
                     KLGRLPKEEEVAEALGVDLDDYFKMLKNYGNLSVLSIEDLHELLGEDRDRIMRYVTEE
                     GDDPEKCAEALELERIMAGEFDKLPERQRYVLSLYYHEDLNMKEIARVLSITEARVCQ
                     IHSQAILNLRVSMKKYLRN"
     gene            579..1478
                     /locus_tag="PKJ17_08810"
     CDS             579..1478
                     /locus_tag="PKJ17_08810"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOD76114.1"
                     /translation="MEREEILKRLRKIDVLPTFPAVMAEVISVIEDPMSSATDLARTM
                     DPSMAGEVLRCANTAYFGTRNFRKITSIEHAIAIVGLEQLSQMILHMPFLSMMGGEGG
                     LDRERFVRHSMICGIVSKVISRTLHAGEEHAVYIGGLMHDIGAIIIYRYFNEEWKVMS
                     ALVRNEGRTWTQAEEDVLSCDHGMIGACLLTMWNIPQAVTDGVMHHHAPHRAAENVWN
                     AKLIGVGNRFAKNVDLSDNFASLDEFINNNKDFIPLVEETGLDVTLSQELDLFEGVYT
                     LMKNARGLLSCAGDVEDDQGTGS"
     gene            1456..>2128
                     /locus_tag="PKJ17_08815"
     CDS             1456..>2128
                     /locus_tag="PKJ17_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD76115.1"
                     /translation="MIKVLVVDDSLVMRKTISRMLSKDKAIEVVGTAVDGRDALEKVE
                     SLKPDVITMDVEMPVMNGIEALKRIMEKDPVPVIIMSALTREGAEITMEALQLGACDF
                     VTKDFTNVGNAFSSKEAEVITKVKNVAKNKVRFLLRKLDIKPKTAVVISPDQKVRHEV
                     LAIGASTGGPPALQHILTRLPRAFPVPIVIAQHMPKIFTQSFSQRLDAVSQLQVKEAE
                     DGETLR"
BASE COUNT          520 a          564 c          642 g          402 t
ORIGIN      
        1 aagctcaaca cctttgccta cctcaggata cggggtgcca tgatcgacga actgcgttcc
       61 cgcgactggt tcccgagaag cgcccggtcg aagtccaaga agataaacga ggtcgtgcgg
      121 aaactggaga acaagctggg acggctgccg aaggaagaag aggtggccga ggcgctgggg
      181 gtggacctcg acgattactt caagatgctc aagaactacg ggaacctctc tgttctcagc
      241 atagaagacc tccacgagct cctgggagaa gaccgcgaca ggataatgcg ctacgtcacg
      301 gaagagggcg acgaccccga gaagtgcgcc gaggccctgg agctggaacg cataatggcc
      361 ggcgaattcg acaagctgcc ggagaggcag cgctatgtcc tgagtctcta ctaccatgag
      421 gacctgaaca tgaaggagat agcccgcgtc cttagcatca ccgaagcccg tgtctgccag
      481 atccattcgc aggcgatcct gaacctgagg gtatccatga agaaatatct gcgcaattaa
      541 aggtttcaag cgggcttccg ataatatgtc tgacagttat ggaacgcgaa gagatcctga
      601 aaagactcag aaagatagac gtgttgccca catttccggc ggtcatggcg gaggtcataa
      661 gcgtcataga ggacccgatg agctccgcca cggaccttgc aaggacgatg gacccctcca
      721 tggcgggcga agtcctgcgt tgtgccaata ccgcttattt cgggactcgc aacttccgca
      781 agatcacgtc cattgagcac gccatagcca tagtcgggtt ggagcagctt tcgcagatga
      841 tcctccacat gccctttctg tccatgatgg gcggcgaggg agggctcgac agggagaggt
      901 tcgtacgcca ttccatgata tgcggcatag tctcgaaggt cataagccgc accctccatg
      961 cgggagagga gcacgccgtc tacataggcg gtctcatgca cgacatcggg gcgatcatca
     1021 tataccgcta tttcaacgag gaatggaagg tgatgagcgc tctcgtgagg aacgagggca
     1081 ggacatggac gcaggcggag gaggacgttc tttcctgcga ccacgggatg ataggggcat
     1141 gccttctgac catgtggaac attccccagg cggtgaccga cggggtcatg catcaccacg
     1201 ctccccatag ggccgccgag aatgtgtgga acgcgaaact catcggtgtg ggaaataggt
     1261 ttgcaaaaaa cgtcgatctg agcgataatt tcgccagtct cgatgaattt attaataata
     1321 acaaagactt cattccgctc gtcgaggaga cgggacttga cgtgacgctt tcacaggagc
     1381 tggacctctt cgagggggta tacaccctca tgaagaacgc gaggggtctt ctatcctgcg
     1441 caggagacgt agaggatgat caaggtactg gtagttgacg attcactggt gatgcgcaag
     1501 acgatatcga ggatgctgtc gaaggacaag gccatcgagg tcgtgggcac tgccgttgac
     1561 ggcagggatg ccctcgaaaa ggtggaatcc ctcaaacccg acgtcatcac catggatgtg
     1621 gagatgcctg tcatgaacgg catcgaagcc ctgaagagga tcatggagaa ggaccccgtg
     1681 ccggtgatca tcatgagcgc cctgaccagg gaaggtgccg agatcaccat ggaggccctg
     1741 cagctcggcg cctgtgattt tgtcacgaag gatttcacca atgtgggcaa tgccttctcc
     1801 tcgaaagagg cggaagtgat cacgaaggtg aagaatgtgg caaagaacaa ggtcagattc
     1861 ctccttcgca agctggacat caagccgaag accgccgtcg tgatcagccc ggaccagaag
     1921 gtccgtcatg aggtccttgc cataggagcc tccacgggcg ggccgccggc cctgcagcat
     1981 atcctgacga ggcttccccg tgccttcccg gtccccatcg tcattgccca gcacatgccc
     2041 aagatcttca cacagtcctt ttcccagagg cttgacgccg tgtcgcagct gcaggtgaag
     2101 gaagcggagg atggggagac gctgcggg
//
LOCUS       DAOPOA010000432         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_56361_length_2125_cov_4.679710, whole genome shotgun sequence.
ACCESSION   DAOPOA010000432 DAOPOA010000000
VERSION     DAOPOA010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56361_length_2125_cov_4.679710"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..>2125
                     /locus_tag="PKJ17_08820"
     CDS             324..>2125
                     /locus_tag="PKJ17_08820"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOD76116.1"
                     /translation="MKKYTIHRDVSPVKTVIDYSRELNEEQLGVVLSDDGPILVIAGA
                     GSGKTRVVTYRVARLLERGVPPGGILLLTFTNKAAREMLHRVEHLVKMDTRYIWGGTF
                     HHIGNLILRQHAERVGYRRSFTILDNEDAKDVVELAIKASGIDAKARMFPKGGVVKEL
                     FSYAANTMQTVDEAIRERSPFFYDIADEMMAVHRIYMEKKRETNAMDFDDLLTYWHRL
                     LAEDEELRKLYAMTFRHILVDEYQDTNRIQSEIIDFMGLLNRNVMVVGDDAQSIYSFR
                     GANFQNILDFPKRYGEAKVYKLETNYRSTPEILDLANNSISFNFNQFEKNLRSMKKSG
                     VIPVVAPSRDVIQQAEFVAQRILELSDEGVPLDRISVLYRAHYHSMEVQMELTRRGIP
                     FEIRSGLRFFEQAHIKDVVSFLRVMSNPDDEVSWKRMLRIFPRIGKKTADHIYAYLKE
                     QSDPFDPLISGRIADTFKGIRKENVEVWSELFGELKDLFSDEAPGDMISAVLKHGYIE
                     YLKLTYPNYEARLEDLGQLINFSTRYHSLETFLSELSLMSGISGEEIVAAGDTDDERV
                     VLSTIHQAKGLEWQVVFIIWCAEGRFPNPRAVEEG"
BASE COUNT          472 a          690 c          588 g          375 t
ORIGIN      
        1 tccatgtagc ccttcgcctt cttgcccagt tcggaccccg ccttgacggc gttgcgcagg
       61 atgtctcccg tggagatctg cacggacccg tcaaactccg tcaacgactt cgcaactgta
      121 cccttgccgg ctcccggcgc tcccaataat acgatacgca tgatagatcc tcctttgcgt
      181 gattaatgat cggacacgaa atttcactga gtttaccaaa aaaatcgaga aaaatcaccg
      241 acaaaaactc tcttcccccc ccttgaccgt cccccgcgtg ccggcttgac atgccggacg
      301 gggcgggggc ataatgtagg cctgtgaaaa agtataccat ccaccgtgat gtctcccccg
      361 tgaagaccgt catcgactac tcccgggagc tcaacgaaga gcagttgggc gtggtcctct
      421 ccgacgacgg gcccatactc gtcatcgccg gggcgggcag cggcaaaacg agggtcgtca
      481 cctaccgggt ggcgcgcctg ctcgagcggg gcgttccacc gggcgggata ctgctcctca
      541 cgttcacgaa caaggcggcc cgggagatgc tccatcgcgt cgagcatctc gtaaaaatgg
      601 acacccgcta catctggggc ggcaccttcc accacatcgg caacctcatc ctgcgccagc
      661 atgccgagcg cgtcggttac cgcaggagct tcaccatcct cgacaacgag gacgcgaagg
      721 atgtcgtgga actcgccatc aaggcatcgg gcatcgacgc gaaggcgcgg atgttcccca
      781 agggcggcgt cgtgaaggaa ctcttcagct acgccgccaa taccatgcag accgtcgacg
      841 aggccatccg ggaacgcagc cccttcttct acgacatcgc cgacgaaatg atggcggtgc
      901 acaggatata catggagaag aagcgggaga cgaacgccat ggacttcgat gacctgctca
      961 catactggca ccggctcctc gccgaggacg aggagctgcg caagctctac gcgatgacct
     1021 tccgtcacat cctcgtcgat gagtaccagg acaccaaccg catccagtcg gagatcatcg
     1081 acttcatggg gctcctgaac cgcaacgtca tggtcgtcgg tgacgacgcc cagagcatct
     1141 actccttccg gggggcgaac ttccagaaca tcctcgattt cccgaaacgt tacggcgagg
     1201 cgaaggtgta caagctggag acaaactacc gcagcacccc ggagatcctg gacctcgcga
     1261 acaactccat ctccttcaac ttcaaccagt tcgaaaagaa cctgcggagc atgaagaaga
     1321 gcggcgtcat ccccgtcgtc gcgccgtcgc gggacgtgat acagcaggcc gagttcgtgg
     1381 cccagcggat actggagctc tccgacgagg gcgtccccct cgacaggata tcggtcctct
     1441 accgcgccca ctatcattcg atggaagtgc agatggagct cacccgccgg ggcatcccct
     1501 tcgagatccg aagcgggctc aggttcttcg agcaggccca tatcaaggac gtcgtctcct
     1561 tcctgcgcgt catgtcgaac cccgacgacg aggtttcatg gaagcggatg ctcaggatct
     1621 ttccccgcat cggcaagaag accgccgacc acatctatgc ctatctcaag gaacaaagcg
     1681 accccttcga tcccctcatc tcgggcagga tcgcggacac cttcaagggc atcaggaaag
     1741 agaacgtcga ggtgtggtcg gagctcttcg gcgagctgaa ggacctcttc agcgacgagg
     1801 ccccggggga catgatctcc gccgtcctca agcacggcta catcgagtac ctcaagctca
     1861 cctatcccaa ctacgaggcg cgtctcgaag acctggggca gctcatcaac ttctcgacga
     1921 ggtaccactc cctggagaca ttcctcagcg agctctccct catgagcggc atctccggcg
     1981 aagagatcgt cgccgccggc gacacggacg atgaacgggt cgtactgagc accatccatc
     2041 aggccaaggg cctcgaatgg caggtcgtct tcatcatctg gtgcgccgaa ggaaggttcc
     2101 cgaaccccag agcggtggag gaagg
//
LOCUS       DAOPOA010000433         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_56406_length_2124_cov_2.921701, whole genome shotgun sequence.
ACCESSION   DAOPOA010000433 DAOPOA010000000
VERSION     DAOPOA010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56406_length_2124_cov_2.921701"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..907)
                     /locus_tag="PKJ17_08825"
     CDS             complement(128..907)
                     /locus_tag="PKJ17_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76117.1"
                     /translation="MLPRRHILDVWELIKDDEDFRSMATITLAIDAIKYMHNEPKKDH
                     LVEALELNEFICFMFPAKRPRNRSLLYHIVSDLLGLLMYGIPDTRKHTIDNIETVNYS
                     EKNMEIYPVIEVWNNLKSKVYKKKHGAEDIIEGFIRKIRVEMDVLERFPFVEETFFES
                     KDTIKEWLPSFASYYDESRKKVRGAYDRWWALWACAGSKEEILGAMVDRLASQAEREY
                     ETVIDKEKVFTSIISDPETNRMENELFSRWYREGVNLLLKI"
     gene            complement(907..1836)
                     /locus_tag="PKJ17_08830"
     CDS             complement(907..1836)
                     /locus_tag="PKJ17_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOD76118.1"
                     /translation="MAGKQDYYELLGVSKTASEDEIKKAYRKLALKYHPDRNPGNKEA
                     EEKFKLINEAYAVLSDPEKRKQFDAFGMGGFQQRYSEEDIFRGFNVGDLFKDLGFGGG
                     DIFSMIFGRQAGRGAKQQRRQQAWDFNDYITRQQQGTQAAQDLDLHYELEIPFMDAIH
                     GAEKRISLATATGTEEVNVKIPKGITSGKKLRLKGKGNQGRSGQRGDLYITMKVAEHP
                     VFKRTGNDLYVTREVKVTDALLGTVVEVPSIDGPKRVTIPAGVKSHSRVRLKGLGVPD
                     QGGDEYVEVIIDIPKKLTDRQKALLEELRKEGL"
     gene            complement(1845..>2124)
                     /locus_tag="PKJ17_08835"
     CDS             complement(1845..>2124)
                     /locus_tag="PKJ17_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76119.1"
                     /translation="MEDVRRLGLHDLHPRIISTGKGNVGLPREDIAGEIERLWKKEGP
                     VIAKGMGNFETISEFDGERQVVYIMKVKCTAVARAVGSDTGTYIAHVR"
BASE COUNT          365 a          619 c          558 g          582 t
ORIGIN      
        1 tctgtcctgt aatggtttca ccttcattgt ttcacactcc tttctgtggt tgttaagtta
       61 gcaattgcga aaatcgattg ccaaaaacaa tataaggacg gtcggaagtt tttgtcaata
      121 ccgggggtta aatttttagc aacaaattta ccccttcccg gtaccacctg ctgaacagtt
      181 cgttttccat ccggttggtt tcggggtcgg aaatgatgga ggtgaagacc ttttccttgt
      241 cgatgacggt ctcgtactcg cgctcggcct gggaggcgag gcggtcgacc atggcgccga
      301 ggatctcctc cttgctgccc gcgcacgccc acagggccca ccagcggtca taggccccgc
      361 ggaccttctt tctgctttcg tcataatagc tggcgaatga aggaagccat tcttttatcg
      421 tgtccttcga ttcgaaaaag gtctcttcca cgaaggggaa acgctcgagg acatccatct
      481 ccacgcggat cttcctgatg aaaccttcta tgatgtcttc ggcgccgtgc ttttttttgt
      541 agactttcga cttgaggttg ttccacacct cgatgacggg atagatctcc atgttctttt
      601 cggagtagtt gaccgtttcg atattatcga tcgtgtgttt gcgcgtatcg gggatgccgt
      661 acatgagaag cccgaggagg tccgagacga tgtggtagag cagcgacctg ttcctcggac
      721 gcttggcggg gaacatgaag cagatgaact cgttgagttc cagggcctcc acgaggtggt
      781 ccttcttcgg ttcgttgtgc atgtacttta tggcgtcgat ggccagtgtg atcgtggcca
      841 tggacctgaa gtcctcgtcg tccttgatga gttcccagac atccaggata tgcctgcggg
      901 ggagcactat agaccttcct ttctaagctc ttcgaggaga gccttttgcc tgtctgtaag
      961 cttcttcggg atatcgatga tgacctccac gtattcgtcg ccgccctgat cggggacacc
     1021 aaggcctttc agacggaccc tggaatggct ttttacaccg gcagggatgg tgaccctttt
     1081 cggcccgtcg atggagggga cctcgacgac ggtccccaga agcgcgtcag tcaccttgac
     1141 ctccctggtc acgtacaggt cgttgcccgt tcgtttgaag acgggatgtt ccgccacctt
     1201 catggttata tagaggtcac cacgttgccc cgaacgcccc tggttgccct tgcccttcaa
     1261 cctcagcttt ttcccgctcg tgatcccttt cggtatcttg acgttcacct cttccgtacc
     1321 cgtcgccgtg gccagcgaga tccgtttttc cgccccatgt atggcgtcca tgaagggtat
     1381 ctccagttcg tagtggaggt cgaggtcctg tgctgcctgc gtgccttgct gctgcctcgt
     1441 gatgtaatcg ttgaagtccc acgcctgctg ccgcctctgc tgctttgccc ccctgcctgc
     1501 ctgcctgccg aagatcatgc tgaagatgtc tcccccgccg aagccgaggt ccttgaagag
     1561 gtctcccacg ttgaaacccc tgaagatgtc ctcttcgctg tagcgctgct ggaagccccc
     1621 catgccgaag gcatcgaact gcttgcgttt ttccgggtcg ctcagcaccg cgtaggcctc
     1681 gttgatgagc ttgaactttt cttccgcctc cttgtttccg gggttgcggt ccggatggta
     1741 cttcagggcc agtttcctgt acgccttctt tatctcgtct tcggaagccg ttttggagac
     1801 gccgagcaat tcgtagtaat cctgctttcc tgccatacgg ttcgtcacct cacgtgagct
     1861 atgtatgtcc cggtgtcgct gccgactgcc cgggcgaccg cggtacattt caccttcatt
     1921 atatatacca cctgacgttc tccgtcaaat tcgctgatgg tctcgaaatt ccccatgccc
     1981 ttcgcgatga cggggccttc tttcttccag agcctttcga tctcgcccgc gatgtcctcc
     2041 ctcggcagac ccacgttgcc ctttcccgta gatatgatgc gggggtgcag atcgtgaagt
     2101 ccaaggcgcc ggacgtcctc catg
//
LOCUS       DAOPOA010000434         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_56629_length_2118_cov_3.230247, whole genome shotgun sequence.
ACCESSION   DAOPOA010000434 DAOPOA010000000
VERSION     DAOPOA010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56629_length_2118_cov_3.230247"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /gene="fmt"
                     /locus_tag="PKJ17_08840"
     CDS             complement(<1..909)
                     /gene="fmt"
                     /locus_tag="PKJ17_08840"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOD76120.1"
                     /translation="MRIVFFGSSAFSVPSLLKIKDSVVAVVTKKARPRGRGYQIDDNE
                     VKSAALSLNVPLVEIGSFRDDEARSIADFHPDLYVVVSFGLIVPKWALDIPSIAPVNI
                     HPSLLPLYRGPSPMQWALLGGDAVTGITFIKMNEKMDEGDIIGQEEVAIEKDDDYTTL
                     SKRLSEKAAQMLQKVVDDIGKHGMMEGTRQDHSAATYAPIITKEMGRIDWTRGAARVD
                     CQVKAFVEWPTAFTYLDGRLLKIYSSFADESVQAQAAAGTVTSITKAGLEVATGKGLL
                     VVRELQLENKKRMKAYDFSIGYRDLKG"
     gene            complement(906..1406)
                     /gene="def"
                     /locus_tag="PKJ17_08845"
     CDS             complement(906..1406)
                     /gene="def"
                     /locus_tag="PKJ17_08845"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOD76121.1"
                     /translation="MSTIDIRVIPDPVLRKLAKPVENITDAVLKLSGDMVETMILAHG
                     AGLAAPQIGLPIRLITIDASLNPTEEKPIILINPVIVETHLEENGEEGCLSVPGYYEY
                     VRRAKKVHARGTDLRGNIVDFECEGQLARAFQHEIDHLNGVLFVDLLSPIKKNIFKKK
                     YTGKKA"
     gene            1584..>2118
                     /locus_tag="PKJ17_08850"
     CDS             1584..>2118
                     /locus_tag="PKJ17_08850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOD76122.1"
                     /translation="MADEQGGKVKVLGFAGSLRKGSFNKMLLNAAVKVAPADVELEVF
                     DIGGIPPFNQDLENEPHEKVRELKEKVRAADGVLIVTPEYNYSVPGVLKNAIDAASRP
                     YGTNPFEGKPVAIMGASIGTLGTARAQYHLRQTCVFLDCFVLNRPEIMVPFAEQKFDA
                     SGNLMDDRTKQMIEELLA"
BASE COUNT          448 a          597 c          585 g          488 t
ORIGIN      
        1 accttttaag tccctgtaac ctatggagaa atcgtatgcc ttcatgcgct tcttgttctc
       61 cagctgaagc tccctgacaa caagcaaacc tttgccggtc gccacttcca ggcccgcctt
      121 ggttatgctc gtcacggtac cggcagctgc ttgtgcctga acgctctcat cggcgaacga
      181 gctgtatatc ttgagcagtc tcccatcgag ataggtaaaa gcggtgggcc actctacaaa
      241 ggccttcacc tgacagtcaa cacgggcggc gcccctggtc cagtctatcc gtcccatctc
      301 cttcgtgatg atgggggcat acgtggccgc cgaatgatcc tgtctcgttc cttccatcat
      361 accgtgcttc ccgatatcat ccaccacttt ctgcaacatt tgtgccgctt tttccgaaag
      421 cctcttagac agggtggtat agtcgtcatc cttctctatc gccacttctt cctggccgat
      481 gatatccccc tcgtccatct tctcgttcat ctttataaac gtgatccccg tgaccgcgtc
      541 gccgcccagg agagcccatt gcatcggcga gggacctcgg tatagcggca aaagggaagg
      601 atgaatattg acgggagcga tggacggaat gtcgagggcc cacttcggca cgataagccc
      661 gaaggacacc acaacataga ggtcggggtg aaagtcggct atggaccgcg cctcgtcatc
      721 cctgaaggag cctatctcga cgaggggcac attgaggctc agggccgcgc tcttgacctc
      781 gttgtcgtcg atctggtatc ccctgcccct ggggcgcgcc ttcttggtca caacggcaac
      841 cactgaatct ttgatcttta gaagggacgg tacggagaag gcggaggatc cgaaaaagac
      901 gatcctcatg ccttcttgcc tgtatacttt ttcttgaaaa tgttcttttt tatgggactt
      961 aaaagatcaa cgaagaggac gccgttgaga tggtcgatct cgtgctggaa cgcgcgcgca
     1021 agctgtccct cgcactcgaa gtcgacgatg ttccccctga ggtccgtacc acgggcatgc
     1081 actttcttcg ctctcctgac gtactcgtaa tacccgggaa cgctcaggca cccctcctcg
     1141 ccgttctcct cgaggtgggt ctcgacgata acgggattga tgagaatgat gggcttctcc
     1201 tccgtggggt tcaaagaggc gtcaatggtt atgagcctga tcgggagccc gatctgcggt
     1261 gccgccagac ctgctccatg ggccaggatc atcgtctcga ccatatcccc ggagagcttg
     1321 aggacagcgt ccgtgatgtt ttcgacaggc ttcgcaagct tcctgagaac aggatctggt
     1381 atgacacgaa tatcaattgt ggacatgcac tattttaaaa ccatccgcgc gcagagtcaa
     1441 taactggaac gccctatctc acattctatc gggtgccttg atcgcggggt gggagctgtc
     1501 gagaggcgag gtctttaccg ttgacaccat tgccgggagg acttagcttc atttcatccc
     1561 acatgccgtg caaggaggtt cccatggccg atgaacaagg tggaaaagtg aaagtgctgg
     1621 gtttcgcggg gagtttgagg aaagggtcct ttaacaagat gcttctcaat gccgccgtca
     1681 aggtcgcgcc ggcggatgtg gagttggagg tattcgacat cggggggatc ccccctttca
     1741 atcaggatct cgagaacgag cctcacgaga aggtcaggga gttgaaggaa aaggtcaggg
     1801 cggccgatgg cgtcctgata gtcacgcctg agtataacta ctccgttccg ggggtcctga
     1861 agaatgccat cgacgccgcg tcgaggccgt acggtacgaa ccccttcgag ggaaaacccg
     1921 tcgccatcat gggtgcttcc atcggcacgc tcgggaccgc cagggcgcag taccatctgc
     1981 ggcagacatg cgtctttctc gattgtttcg ttctcaaccg gcccgagata atggtccctt
     2041 tcgcggagca gaagttcgat gcctcgggca acctcatgga tgacaggaca aagcagatga
     2101 tagaggaact cctggccg
//
LOCUS       DAOPOA010000435         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_56703_length_2116_cov_3.186317, whole genome shotgun sequence.
ACCESSION   DAOPOA010000435 DAOPOA010000000
VERSION     DAOPOA010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56703_length_2116_cov_3.186317"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKJ17_08855"
     CDS             complement(<1..697)
                     /locus_tag="PKJ17_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HOD76123.1"
                     /translation="MALFENYDRRIAQISAALGKYGIKSIEDAKAVCDAKGIDPYKIA
                     KETQPICFENAGWAYVVGAAIAIKKGCTKAADAAEAIGEGLQAFCIPGSVADDRKVGL
                     GHGNLAAMLLREETKCFAFLAGHESFAAAEGAIGIAKSANKVRKEPLQVVLNGLGKDA
                     AQIISRINGFTYVRTKFDYHSGNLEIAETRPYSKGDRAAVRCYGADDVREGVAIMQHE
                     KVDVSITGNSTNPT"
     gene            complement(712..1404)
                     /locus_tag="PKJ17_08860"
     CDS             complement(712..1404)
                     /locus_tag="PKJ17_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76124.1"
                     /translation="MMYSQEVEKMCSVTKGADHGPAPIPQEGKWTQVKEVKDICAFTH
                     GIGWCAPQQGTCKLTLNVKDGIIEEALVEALGCSGMTHSAAMAAEILPGKTILEALNT
                     DLVCDAINTAMRELFLQIVYGRSQSAFSEGGLAIGAGLEDLGKGLRSQVATMYGTVAK
                     GPRYLEMAEGYVRKIALDDNNEIIGYEFVSLGRMMEMIAKGMDANEALQKASGKYGRV
                     DEAVKLIDPRHE"
     gene            complement(1643..>2116)
                     /locus_tag="PKJ17_08865"
     CDS             complement(1643..>2116)
                     /locus_tag="PKJ17_08865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HOD76125.1"
                     /translation="HVLSLLREVRSQDFPELVNAKIKVLFDLKKRKSGGMIVLARIIK
                     TNDLMRHLTKNGGDAIEGYDYIITLDKKCWDNTTDLDRVKILRHELRHTFFDIESEDN
                     PYKLLNHSISDFYEEVELNKNDPRWRERVATLTEDIYEQEKEARAEKKNKKIKTA"
BASE COUNT          360 a          632 c          579 g          545 t
ORIGIN      
        1 gggtggggtt cgtcgaattg ccggtgatgg agacgtcgac cttctcgtgc tgcatgatgg
       61 ccacgccctc acggacatca tcggcgccat agcatctgac ggcagccctg tcacccttcg
      121 aatagggccg ggtttccgcg atctcgaggt ttcccgagtg gtagtcgaat ttcgtccgca
      181 cgtaggtgaa cccgtttatc cgggagatga tctgtgccgc gtccttcccg agaccgttca
      241 ggacgacctg caggggctcc ttgcggacct tgttggctga tttggcgatg ccgatggcgc
      301 cttcagcggc ggcaaaggac tcatgccccg ccagaaaggc aaagcacttc gtctcttccc
      361 tcaaaagcat ggcggccagg ttcccgtgtc ccaggcccac cttcctgtcg tctgccacgg
      421 aaccggggat gcaaaaggcc tgcagtcctt cgcctatagc ctcagccgcg tctgccgcct
      481 ttgtgcaccc cttctttatc gcgatggccg cgccgacgac atacgcccat ccggcgttct
      541 cgaagcatat cggctgggtc tccttcgcta tcttgtaagg gtctatgcct ttggcgtcac
      601 agacagcctt cgcgtcctct atcgacttta taccgtactt gccgagcgcg gcgctgatct
      661 gcgcgatcct tctatcgtag ttctcgaaaa gtgccatgtc gtcatccctc cttattcatg
      721 cctggggtcg atcagcttca cggcttcgtc gacccttccg tacttcccgg aagccttctg
      781 aagggcctcg ttggcgtcca tgcccttcgc tatcatctcc atcattctgc ccaggctgac
      841 gaattcgtag ccgattatct cgttgttgtc gtcgagagct atctttctca cgtagccctc
      901 ggccatctcg aggtaccggg ggccctttgc cacggtgccg tacatggtgg ccacctggct
      961 gcgcaggccc tttcccaggt cctcgagccc ggcgcctatc gccagaccgc cttcggagaa
     1021 tgccgactgg ctcctgccgt aaacgatctg gaggaaaagt tccctcatcg ccgtattgat
     1081 cgcgtcgcac acgaggtccg tgttgagcgc ctcgagtatg gtcttgcccg gcagtatctc
     1141 ggcggccatg gcagccgaat gggtcatgcc cgaacatccg agcgcctcga caagagcctc
     1201 ctcgatgatc ccgtccttta cgttgagcgt cagcttgcac gtcccctgct ggggggcgca
     1261 ccagcctatg ccgtgcgtga acgcgcatat atccttaact tcctttacct gggtccactt
     1321 gccctcctgc ggtatcggcg ccgggccgtg gtccgcccct ttcgtcacgg agcacatctt
     1381 ctccacttcc tgagagtaca tcatgagcgt atatcctcct tggttggttt cttgcggtta
     1441 gtcaacgtga aaagacgaag cgcctatagt atactacctt ggcggggctg atgtcatggg
     1501 ggaaagaggg ggaaacggct ttaatagctt gacggcttga gtcgcttgaa tagcttgaat
     1561 ggctttaaag gcaaagacga ataaccttct tctttcaaag aagaggtctg gaagcccttg
     1621 actctcaagc gactcaagcc attcaagcgg tctttatctt tttgttcttc ttttccgctc
     1681 tggcttcttt ttcctgttcg tagatgtcct cggtgagggt ggcgacgcgt tcgcgccagc
     1741 gggggtcgtt cttgttgagc tcgacctctt cgtagaagtc ggatatgctg tggttgagaa
     1801 gcttgtacgg gttgtcttcg gactctatgt caaagaaggt gtgccgcaat tcgtggcgca
     1861 gtatcttgac cctgtcgaga tcggtggtgt tgtcccagca tttcttgtcg agggtgatga
     1921 tgtaatcgta cccctcgatg gcgtcgcccc cgttcttcgt gaggtggcgc ataaggtcgt
     1981 tggtctttat gatccgggcg aggacgatca tcccgcccga cttcctcttt ttcaggtcaa
     2041 agagaacctt tatcttcgcg ttgaccagtt cgggaaaatc ctgcgacctc acctccctga
     2101 gcaggctcag aacgtg
//
LOCUS       DAOPOA010000436         2115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_56721_length_2115_cov_6.862621, whole genome shotgun sequence.
ACCESSION   DAOPOA010000436 DAOPOA010000000
VERSION     DAOPOA010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56721_length_2115_cov_6.862621"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PKJ17_08870"
     CDS             <1..563
                     /locus_tag="PKJ17_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD76126.1"
                     /translation="HDLLVVGAHGRHPVRTLALGTTAQRLLGKTRTPVLVVKGKPDRP
                     YRRILVAVDFSPNSQMAMEGCSAIAPEALVRVVHVYEPLLERQMISAGASDKVLEEYR
                     AKARIDAETQMTAFLEAGQWSTGRTLQHVEYGHASARLPEIAHEWGPDLVVVGKHGRS
                     RVEELLLGSVTIHLLSQSRCDVLVAQ"
     gene            614..2089
                     /locus_tag="PKJ17_08875"
     CDS             614..2089
                     /locus_tag="PKJ17_08875"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOD76127.1"
                     /translation="MIPIEHILLIGSLLILVSIGVARLSENLGVPALLLFLGIGMLAG
                     SEGPGGIYFDDAPLAQSVGVIALVFILFAGGLDTKWSEVKPSFFPAVSLATVGVLLTA
                     TGVGLFAFFALKLPFLTALLLGAVVSSTDAAAVFSVLRSKNISLKGRLRPLLELESGS
                     NDPMAVLLTIGIIQIIVNPGASFGSIALLFVQQMGLGVVFGFFFGRATVILLNRLKSY
                     YEGVYPVFFLALAVLTYGLTAAAGGSGFLAVYIAGLIAGNSDFIYKKSLLRFFDGLAW
                     LSQIAMFLTLGLLVFPSRIVPVVWAGLACSAFLMFVARPAGVFLSLLFARLQWREKVL
                     VSWVGLRGSVPIVLATFPLLAGVAGSETIFNIVFFIVLTSTLLQGWSIPVVARLMKLQ
                     APLEMKRHYPIEFAPIDGVDTQLVDFIIPFNAAIAGKAIVELGLPPDSLIVLVNRDEK
                     FFVPSGGTVLEGGDVVLVLVNDRNISEVREKLMRWKEPVKE"
BASE COUNT          369 a          645 c          600 g          501 t
ORIGIN      
        1 atcacgacct gctcgtggtg ggtgctcacg gtcggcaccc ggtacggacc ctggcccttg
       61 gcactaccgc ccagcgcctt ctgggcaaaa cgcgtacacc tgtccttgtt gttaagggca
      121 agcctgacag gccgtatcgg aggatcctcg ttgccgtcga tttctcaccg aattcccaaa
      181 tggccatgga gggttgttcc gcgatagccc ccgaagccct cgtcagggtg gtgcacgtct
      241 acgagccgct cctcgaaagg cagatgatct ccgccggggc atccgacaag gttctcgaag
      301 aatatcgggc aaaagcacgg atcgacgccg agacacagat gaccgccttc ctggaagccg
      361 ggcagtggag cacaggaaga acgctgcaac acgttgaata cggacatgcg tcagcgagac
      421 tgccggagat agctcacgag tggggtcccg atctcgtggt cgtgggcaag cacgggaggt
      481 cacgcgttga agaactcctg cttggcagcg tgacgataca cctgttgtcc cagtcgcgat
      541 gcgacgtgct cgttgctcaa tagtcttcgc ggtgatagca tatggttttg gataaagctg
      601 agacgattgc cgtatgatac caatcgaaca cattctcctt ataggttcgc tccttatcct
      661 ggtcagtatc ggggtcgcga ggctatccga gaaccttggg gtcccggctc tgctcttgtt
      721 tctcggcata gggatgcttg ccggttccga aggtcccggc gggatctact tcgacgatgc
      781 ccccctcgcg caatccgttg gtgtcattgc gctcgtattc atccttttcg cgggaggtct
      841 cgacacaaag tggtcggagg tcaagccatc cttcttcccg gcagtgagcc tggcgaccgt
      901 cggagtcctg cttacggcga ccggggtcgg cctcttcgcc ttctttgccc tgaagctccc
      961 gttcctcacc gccctgctcc tgggggctgt tgtgtcatcg actgacgccg cggcggtctt
     1021 ttccgttctc cggtccaaga atatcagcct caagggccgc ctgagacccc tgctcgaact
     1081 tgaatcgggc agcaacgatc ccatggcggt cctcctcacc atagggatca ttcagatcat
     1141 agtgaaccct ggtgcctcgt tcgggtccat cgccctcctg tttgtccagc agatggggct
     1201 cggggtcgtc ttcgggttct tcttcggaag agcgacggtc atattgctca atcgattgaa
     1261 gtcctattac gaaggcgtct atcctgtttt cttccttgcc ctagccgtgc tcacctacgg
     1321 cttgacggcg gccgcggggg gaagcggctt tcttgccgtg tacattgctg gcctgattgc
     1381 gggcaacagt gacttcatct ataagaagag cctgctgcgc ttcttcgacg gccttgcctg
     1441 gctcagccag atcgccatgt tcctcaccct cggcctcctg gtgtttccat cccgtatcgt
     1501 ccccgtcgtt tgggccggcc ttgcctgttc ggcattcctc atgttcgttg cccgtcccgc
     1561 gggagtcttc ctttccctcc tctttgcccg ccttcaatgg agggaaaagg tcctcgtttc
     1621 ctgggtgggc ctgagagggt ccgtgcccat tgtcctcgcg accttccctc ttttggcagg
     1681 tgtggccggc tccgagacga tattcaacat cgtcttcttc atcgtgctca cctcaaccct
     1741 gctccagggc tggtcgatcc ccgtcgttgc ccgcctgatg aagctgcagg cgccccttga
     1801 aatgaaaaga cattacccca tcgaatttgc tcccatcgac ggtgtcgaca ctcaactcgt
     1861 cgacttcatc attcccttca atgcagcgat cgccggcaag gcaatcgtgg aactcggtct
     1921 tccgcccgac agcctcatcg ttcttgtgaa ccgcgacgag aagttctttg tgcccagcgg
     1981 cggtaccgtt ctcgaaggag gggatgtggt cctggtcctg gtcaacgaca ggaacataag
     2041 cgaggtccgg gagaaactca tgaggtggaa agagcccgtg aaggaatgac ggaccggcag
     2101 gtgcttgcac gaggc
//
LOCUS       DAOPOA010000437         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_56799_length_2113_cov_3.607386, whole genome shotgun sequence.
ACCESSION   DAOPOA010000437 DAOPOA010000000
VERSION     DAOPOA010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56799_length_2113_cov_3.607386"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="PKJ17_08880"
     CDS             <1..1319
                     /locus_tag="PKJ17_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD76128.1"
                     /translation="NDALQGLIREGRPYDVEFKIRRPDTAEVVDIHSVAEYDGDRHVV
                     FGIIQDITDRKELESQLLQARKLEAIGTLAGGIAHDFNNILMGIEGYASLMMSEKTDA
                     GHPDLERLKSIEEQVKSASNLTRQLLGFARVGRYEIRPADMNEIVRETSAMFHRTRKE
                     LTIHEEYERQPWTVEVDRGQIEQVLLNLYLNAWHAMPAGGEIRLTTANMVVDGIFASL
                     HGIPTGKYVRVSVADTGMGMGEETMKRIFDPFFTTKEMGRGTGLGLAMVYGIMKGHKG
                     LVDVRSKPGQGATFTLYFPASEKKLLRERRTVSKALRGSEAILLVDDESTVLRVTRRM
                     LESLGYTVHAMNGGSDAVSFLKDAKCAVDLVLLDMVMPGMSGSETFDRIRETNPSVKV
                     ILSSGYSMDSEARMIMGKGCAGFIQKPYNIALLSRKIREVLESEDP"
     gene            1504..2031
                     /locus_tag="PKJ17_08885"
     CDS             1504..2031
                     /locus_tag="PKJ17_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HOD76129.1"
                     /translation="MKKLMILMVIIGFALAGCATRTGTGAAVGAGVGAAVGAGAGQAI
                     GRDTSSTLIGAGIGAVVGGLTGAAIGRYMDNQEQSMRQAIANSEAATMRREQEIVAGA
                     RKQSVDVLTVTFKSDYLFAVNSSTLLPGAQGELKGWRTCSGSTPKQISRSPGIPTAAD
                     RLTITRSSPSAARKR"
BASE COUNT          491 a          603 c          647 g          372 t
ORIGIN      
        1 tcaatgacgc cctgcaaggc ctcatcaggg aagggcgccc ttatgacgtg gagttcaaga
       61 tacggcgccc cgacacggcg gaagtggtgg atatacactc cgtggcggag tacgacggcg
      121 acaggcacgt ggtgttcggg atcatccagg acatcaccga ccgcaaagag ctcgagtcgc
      181 agcttctcca ggccaggaaa ctcgaggcca tcggcaccct ggcgggcggc attgcccacg
      241 atttcaacaa cattctcatg gggatagagg gatacgcctc gctgatgatg tcggaaaaga
      301 cggatgccgg ccatcccgat ctcgagcgtc tcaagtccat cgaggaacag gtcaagagcg
      361 cgtccaacct cacccgccag cttctgggct ttgcccgggt ggggagatat gagatacggc
      421 ctgccgacat gaacgagata gtccgcgaga cctccgccat gttccacaga acgagaaagg
      481 agttgacgat ccacgaggaa tacgaaaggc agccgtggac cgtggaggtc gaccggggcc
      541 agatagaaca ggtcctgctc aatctctacc tcaacgcgtg gcatgccatg cccgcggggg
      601 gagagatccg cctcacgact gcaaacatgg tcgtcgacgg gatctttgct tccctccacg
      661 gcattcctac cgggaaatat gttcgggtca gcgtcgcgga tacgggaatg ggcatgggag
      721 aagagacgat gaaacgcata ttcgatccct tcttcacaac gaaggagatg ggcaggggga
      781 caggcctcgg cctcgcgatg gtgtacggga tcatgaaggg acataagggt cttgtggatg
      841 tcaggagcaa accgggccag ggagccacgt tcaccctcta ttttcccgca tcagagaaga
      901 aactcctccg ggaaaggcga acggtatcga aggccctgag gggttccgaa gcgatcctcc
      961 ttgtcgacga cgaatcgacg gtccttcggg tgacgcggag aatgctcgag tctctcggat
     1021 acaccgtgca cgccatgaac ggcggatcgg acgcggtctc cttcctcaag gatgccaaat
     1081 gcgccgtcga cctcgtattg ctcgatatgg tcatgcccgg catgtccggc agcgagacct
     1141 tcgaccgcat cagggagacg aacccctctg tgaaggtcat cctctcgagc ggttacagca
     1201 tggacagcga ggcccgcatg atcatgggca agggatgtgc cggcttcatc caaaaacctt
     1261 acaacatcgc cctcctgtcc cggaagatcc gggaagtgct cgagagcgag gatccgtgag
     1321 tcttaaccca ggcggcaagg acctctttcc cgtctctctg atatcccaat ccctcgcaga
     1381 tccgtcctcg ctccccgcgg tataatcatc catacgcaac cggcacagaa tgcagttgac
     1441 aagggccgga tccgtcgtag actgttcata aggatggaca atcgacatga agggggtgac
     1501 aggatgaaaa aactgatgat cctgatggtt attatcggtt tcgcacttgc cggctgcgcg
     1561 acaagaacag ggacaggtgc cgcggttggt gccggtgtcg gtgcggcggt cggggcaggg
     1621 gccggtcagg ccattggacg cgacacctcc tcgacgctca ttggcgcagg catcggagcc
     1681 gtcgtcggag gcctgacggg agcggcaata ggcagataca tggacaacca ggagcagtca
     1741 atgcgtcagg ccattgccaa ttccgaggcc gcgacaatga gacgggaaca ggagatcgtt
     1801 gcgggagcca ggaagcaatc cgttgacgtg ctcacggtga cctttaaatc cgattatctt
     1861 ttcgcggtca attcctcaac cctgctcccc ggtgcccagg gcgaactgaa agggtggcgg
     1921 acgtgctcag gcagtacccc gaaacaaata tccagatcgc cgggcatacc gacagcagcg
     1981 gatcgactga ctataaccag aagctctccg agcgccgcgc ggaagcggta aggaacgccc
     2041 tggcggggat gggtgtcaac acgtctcgcc tgaccaccat cggttacggc gaaacgagac
     2101 ccatcgcaag caa
//
LOCUS       DAOPOA010000438         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_56836_length_2112_cov_4.914439, whole genome shotgun sequence.
ACCESSION   DAOPOA010000438 DAOPOA010000000
VERSION     DAOPOA010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56836_length_2112_cov_4.914439"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..905)
                     /locus_tag="PKJ17_08890"
     CDS             complement(72..905)
                     /locus_tag="PKJ17_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD76130.1"
                     /translation="MKDILKRTVRHHLALVGLIILVPMFFCAVFAPVISPHDPIEPDI
                     KNILSAPTFSHPFGTDTLGRDVFSRVLYGTRISLLVGFVSVGIAILIGLILGAISGYF
                     GGMVDEVIMRFVDLMMCFPTFFLILAVIAILEPSIWNIMIVIGLTSWMGTARLVRAEI
                     LSIKSKEYVMAAKAQGFNSLRIIFRHVLPNAVTPVYVVATLGIGGAILTESALSFLGI
                     GVQPPTPSWGNILTQAKDNIEVAWWLSLYPGLAIFLTVMGYNLLGEGLRDVFDPRRKG
                     A"
     gene            complement(939..>2112)
                     /locus_tag="PKJ17_08895"
     CDS             complement(939..>2112)
                     /locus_tag="PKJ17_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD76131.1"
                     /translation="SEDLLEAALHPEDSPFHDHFAGRLREEFGLGGERFAGFSLTYLS
                     QALAAFAMMGFVRSEFPGVKIILGGGLVTSWMSASAWKNGFGGLIDHVVAGPGEAFLL
                     GLLGSPPEAGGRKISTSYACDAFPLKDYLSPGMIMPYSTSAGCYWGKCSFCPETAEGT
                     PYAQLAPIEAAAGLRELAEEYRPALAHIVDNAISPSVLKALVNEPPGVPWYGFARVTK
                     ELTDEGFCRDLRASGCVMLKLGVESGDQDVLDGMRKGIGISDVSKALKALTRAGIGTY
                     VYLLFGTPGEDEEAARRTMDFIVEHHEFIGFVNLAVFNLPHSSPDAAALDAKPFYGGD
                     LSFYTDFVHPKGWDRRKVRRFIDREFTRHPAIREIVNRQPPYFTSNHAPFFVDAFC"
BASE COUNT          445 a          613 c          671 g          383 t
ORIGIN      
        1 ggaaaagagg gccggctggt ggggtttatc aacaggctgt caagcgattc aagccattca
       61 agccctttct ttcaggctcc cttgcgcctt ggatcgaaga cgtccctcaa gccttcgccg
      121 aggaggttgt agcccattac ggtgaggaag atggcaaggc cggggtagag ggagagccac
      181 caggccacct ctatgttgtc cttcgcctgg gtgaggatgt tgccccagct cggtgtgggg
      241 ggctggacgc cgatgccgag gaaggacagc gccgattcgg tcaggatggc cccgccgata
      301 cccagcgtcg cgacgacgta gacgggggtc accgcgttgg gcaggacgtg gcggaagatg
      361 atcctcaggg aattgaagcc ctgcgccttc gcggccatga catactcctt gctctttatg
      421 ctcagtatct ccgcccggac gaggcgcgcc gtgcccatcc agctcgtcag cccgatgacg
      481 atcatgatgt tccagatgct tggttccagg atggcgatga cggcaaggat gaggaagaac
      541 gtcgggaaac acatcatgag gtcgacgaag cgcatgatga cctcgtccac catccccccg
      601 aaataacccg atatggcgcc gagaatgagc cctatgagga tggcgatgcc cacggaaacg
      661 aatcccacga ggagggatat ccgcgtcccg tagaggaccc gggagaagac gtcccgtccc
      721 agcgtgtccg tgccgaaggg atgggagaag gtaggcgccg agaggatgtt cttgatgtca
      781 ggctcgatgg ggtcgtgagg ggaaatgacc ggcgcgaaaa cggcgcagaa gaacatgggc
      841 acgaggatga tgagccccac gagcgcgagg tgatggcgta ccgtcctctt aaggatatcc
      901 ttcatgtcac ctcatattaa tagccgcagg atttaaaatc aacaaaaagc gtctacgaag
      961 aaaggcgcat gattggatgt gaagtagggg ggctgccggt tcacgatctc ccgtatggcg
     1021 gggtggcgtg tgaattccct gtcgatgaag cgcctcacct tcctgcggtc ccatcccttc
     1081 ggatggacga agtccgtgta gaacgagagg tcgccgccgt agaaaggttt cgcgtcgagg
     1141 gcggcggcgt cggggcttga gtggggaagg ttgaagacgg cgaggttgac aaacccgatg
     1201 aactcatggt gctccacgat gaaatccatg gtcctgcgag ccgcctcctc atcctctccg
     1261 ggtgtcccga aaaggaggta gacgtaggtg ccgatacccg cccgcgtaag tgctttcaag
     1321 gccttcgaca cgtcggagat tccgatccct ttgcgcatgc cgtcgaggac gtcctggtct
     1381 cccgactcga cgccgagctt gagcatgacg cagccggaag ccctgaggtc cctgcagaaa
     1441 ccctcgtccg tcagctcctt cgtcacccgg gcaaagccgt accagggaac gccgggcggt
     1501 tcattgacga gcgccttgag gacggaaggg cttatcgcgt tgtcgacgat gtgtgccagg
     1561 gcgggccggt actcctcagc aagctctctc agcccggccg cggcttctat cggcgcgagc
     1621 tgcgcgtatg gggtgccttc ggccgtctcg ggacagaagg agcatttgcc ccagtagcat
     1681 cccgccgatg tgctgtaagg catgatcata cccggcgaca ggtaatcctt caaagggaat
     1741 gcatcgcagg cataggaggt ggagatcttc ctgcctcccg cctcaggcgg cgatccgaga
     1801 agtcctagga gaaaggcctc cccgggaccc gcaacgacat ggtcgatgag gccgccgaaa
     1861 ccattcttcc atgccgacgc gctcatccat gacgtcacca gcccgccgcc gagaatgatc
     1921 ttcaccccgg ggaactcact cctcacgaaa cccatcatcg cgaaggcggc aagcgcctgg
     1981 ctgaggtagg tgagcgagaa tccggcgaac ctctccccgc cgagcccgaa ttcctccctg
     2041 agacgccccg cgaaatgatc gtggaaagga ctgtcctcgg gatgaagcgc ggcctcaagg
     2101 aggtcttcgc tc
//
LOCUS       DAOPOA010000439         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_56981_length_2108_cov_3.786167, whole genome shotgun sequence.
ACCESSION   DAOPOA010000439 DAOPOA010000000
VERSION     DAOPOA010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56981_length_2108_cov_3.786167"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..869
                     /locus_tag="PKJ17_08900"
     CDS             66..869
                     /locus_tag="PKJ17_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76132.1"
                     /translation="MPYESCCLGSINLVKFVKDGDVDRERLASLVHLAVRFLDDVIDA
                     GKYPVAMIEDISKANRKIGLGVMGFAHMLIALGIPYDSADALKMAGSVMGLIQEESKK
                     ASRDLAGERGAFPNYRGSIWDGRGLPQRNATTTTIAPTGTLSIIAGTSSGIEPVYDTE
                     YSRIIFGGLKVHVLDPLYEQMKDTVDPSSLKKLFRKAHDVSPDWHLAIQKAFQDHVDN
                     AVSKTINLPAVTTPEEVRSIFLNAHAMGLKGITVFRDTSRDSQVLSCKC"
     gene            986..1447
                     /locus_tag="PKJ17_08905"
     CDS             986..1447
                     /locus_tag="PKJ17_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD76133.1"
                     /translation="MLKPTKILVPTDFSGYSDAALRQAVDIAEEYGAEVHVLHVVEPR
                     IHTMYEYEFAGKKASPEGIRNLETRLVKSAEGRMLNYLEQIGSGRKVKVFPRVVKGQP
                     VTEILNFQKDRGIDLIVISSLGHGGLAEYFTGSVARNVLKGATCPVLLTKS"
     gene            1498..1944
                     /locus_tag="PKJ17_08910"
     CDS             1498..1944
                     /locus_tag="PKJ17_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD76134.1"
                     /translation="MLKPTKILVPTDFSEYSDKALQQGIDIARQYNAQVYLFHVIPME
                     ITHVVVDYAIPVEAIESFEKQQVDTAMENMKAQLAKFPKAGEVQVFMEIGKGVAYEEI
                     LKEEKKKGIDLIVIASLGRTGLAKYFIGSVSRNVLRHAECPVLLTK"
BASE COUNT          511 a          605 c          605 g          387 t
ORIGIN      
        1 gggcgaatcc cacaccgcag gcgggcgcca tagaggccac gaacccctgc tgagaacaac
       61 ccctcctgcc ttacgaatcc tgctgcctcg gttccatcaa tctcgtgaag ttcgtgaaag
      121 acggggatgt ggaccgggaa agactggcaa gcctcgtaca cctcgcagtg cggttcctcg
      181 atgacgttat agacgcgggc aagtatcccg tggcgatgat cgaagacatc tccaaggcca
      241 acaggaagat cgggctcggc gtcatgggtt tcgcccacat gctcatcgct ctcggcatcc
      301 cctacgattc cgcagatgcc ctgaagatgg cgggcagcgt catgggtctt atacaggaag
      361 agtcgaagaa agcatcccgg gatttggccg gtgaacgggg cgcctttccc aactacaggg
      421 gaagcatatg ggacggcaga ggcctcccgc aacgcaacgc cacgacgacg accatcgcgc
      481 cgacaggcac gctcagcatc atcgccggca cctcgagcgg catcgagccc gtctacgaca
      541 ccgagtattc gcggatcatc ttcggcggcc tcaaggtcca tgttctggac cccctgtacg
      601 aacagatgaa ggacaccgtg gacccctcaa gcctgaaaaa actcttcaga aaggctcacg
      661 acgtgagccc cgactggcac ctggcgatcc aaaaggcctt ccaggaccat gtggacaatg
      721 ccgtttcgaa aaccatcaat ctccccgcgg tcacgacgcc cgaagaggtg cgatccatat
      781 tcctcaacgc tcacgccatg ggcctgaagg gcatcaccgt cttccgcgac accagcaggg
      841 actcccaggt cctgtcgtgc aagtgctgac gggtcagcgc cagggcgctt gcgcccccct
      901 gtgctataat taagttagaa ggcggttgcc atgcagcacg ggagcccagg ggcaggaaga
      961 tcgcaaagaa aggaggtcag acgccatgct aaaacctaca aagatcctgg tacccaccga
     1021 tttttcaggt tactctgacg ccgctctcag gcaggccgtc gacatagcgg aggaatacgg
     1081 agccgaggtg cacgtccttc acgtggtgga accaaggata cacacgatgt acgaatatga
     1141 gtttgcgggc aagaaggcct cgcccgaagg catcaggaac cttgagacca ggctggtgaa
     1201 gtccgcggaa ggccgaatgc tcaactatct ggagcagata ggctcgggga gaaaggtgaa
     1261 ggtcttcccc agggttgtga aaggtcagcc tgtcacggaa atactcaatt tccagaagga
     1321 caggggcatc gacctcatcg tcatatcttc cctgggccac ggcggccttg cggagtattt
     1381 cacgggctcg gtggcccgca acgtcctgaa gggtgccacc tgtcccgtgc tcttgacgaa
     1441 gagctgaccc gggttaggcg ctcaacgccc ctcacgggga cccgaaagga gggagtcatg
     1501 ttgaaaccaa caaagatcct ggtgccgacg gatttttcgg agtattccga taaggccctc
     1561 caacagggca tcgatattgc ccggcaatac aatgcccagg tgtatctttt ccacgtcatc
     1621 cccatggaga tcacacacgt cgtcgtcgat tacgccatcc ccgtggaagc gatagaaagc
     1681 ttcgagaagc agcaggtgga caccgccatg gagaacatga aagcgcaact ggccaagttt
     1741 ccaaaggccg gagaggtgca ggtcttcatg gagatcggga agggtgtcgc ctatgaggag
     1801 atcctcaagg aggaaaagaa gaaaggcatc gatctcatcg tcatcgcctc cctgggaaga
     1861 acgggcctcg ccaaatactt cattggctcc gtatccagaa acgtcctgag acacgccgag
     1921 tgcccggtgc tgctgaccaa gtaacggaac ggctgcttga gtcgcttgaa tggcttgagt
     1981 agcttgaagg tcagagacgg caagcaaaac caccgtctca taagagggga ttgttcctgg
     2041 tcttgaacac tcaacaggct gttgacaaag tgatttctga agacgattcg tgctgtttcg
     2101 tcattccg
//
LOCUS       DAOPOA010000440         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57016_length_2107_cov_3.624269, whole genome shotgun sequence.
ACCESSION   DAOPOA010000440 DAOPOA010000000
VERSION     DAOPOA010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57016_length_2107_cov_3.624269"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKJ17_08915"
     CDS             complement(<1..496)
                     /locus_tag="PKJ17_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD76135.1"
                     /translation="MIGISKLYCGAVEASDPLRYGRDSSLLPSHLLQFSKDKRPVVVW
                     NTTKRCNLRCVHCYAFAEGEDYRGDELSTDEGKALIDDLARFRVPVILFSGGEPVLRE
                     DLPELIDHAVKKGVRAVISTNGTLITKEKARVFARCSLSYIGVSLDGLTEINDAFRGV
                     EGAFA"
     gene            609..687
                     /locus_tag="PKJ17_08920"
     tRNA            609..687
                     /locus_tag="PKJ17_08920"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Pro,seq:tgg)
     gene            complement(831..1484)
                     /locus_tag="PKJ17_08925"
     CDS             complement(831..1484)
                     /locus_tag="PKJ17_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOD76136.1"
                     /translation="MDLIEAIKVRKSARAFKPDAVSRQQIEEILSLVVRAPSAINLQP
                     WEFIVVMGEEKERLSRRLVKLYREKQISCSPGNVKPLSAEFHKRGAESFILMNPYLEK
                     MGQEFNRFINEGSCNFYGAPSAVIFCLDNAFSEARLVDIGIALGYFVLIAHNFGLSTC
                     PIGLINAYEDDIKDMLNIPDNKDVVIGAALGYPDLASPINEFTSPREDLENFVRWID"
     gene            complement(1521..>2107)
                     /locus_tag="PKJ17_08930"
     CDS             complement(1521..>2107)
                     /locus_tag="PKJ17_08930"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOD76137.1"
                     /translation="VDDLRVHFSFERPQDHPNIKAWREAFKKMGMAPTKYPSFIESLL
                     KRALKGGPFPRFNPIVDLYNSIALKYLVPISGHAISGVDGNISLTFAAGTETFIPMDM
                     GEQEAVDKGEVIVKDDRDALTRRWLWKRSNKDRIESETTRACLLIHAMSGLPDWLCER
                     AAKDLVDSILVNEYGRIVHRATVTASQRETEFAV"
BASE COUNT          430 a          616 c          584 g          477 t
ORIGIN      
        1 gcgcgaaggc accctcgacc ccgcggaagg cgtcgttgat ctccgtcaga ccgtcgaggc
       61 tgacaccgat gtaggacagc gaacaccggg cgaacacccg ggccttctcc ttcgtgatga
      121 gggtaccgtt cgtcgatatg acggccctga cgcccttttt caccgcgtgg tcgatgagct
      181 ccggaaggtc ctccctcagc accggctccc ctccggagaa gagaatgacg ggcacacgga
      241 aacgagccag gtcatcgatg agcgctttgc cctcgtccgt cgacaactcg tcgcccctgt
      301 aatcttcccc ctcggcaaaa gcgtagcagt gaacgcacct gagattacag cgcttcgttg
      361 tgttccacac aacgacgggt ctcttgtcct ttgagaactg cagaaggtgt gacggcaaaa
      421 gcgaagaatc cctgccgtac ctcagggggt cggaggcctc tacggccccg cagtagagtt
      481 tggatatgcc gatcatggtg cccttattgt gagccgaaac gtgaaaaaag tcaacgccgt
      541 tgtccctcac cctgtaaggg cccgacttat tgaaatgcga ctttttctgt gatatgctgg
      601 tggctgatcg gggcgtggcg cagtatggtt cagcgcgctt gccttgggcg caagaggccg
      661 ctggttcaaa tccagtcgcc ccgaccagaa aaccggctta aaccgcttga accgcttgaa
      721 cgtccgaggc gaatggaggc ttttcacgcg gtgttcggtt atatcacgcg attctttcct
      781 ttttcttacc ctcaaacgct caagcggttc aagcgattca agccgtcttt ctagtctatc
      841 cacctcacga agttctcaag gtcctcacgg ggagaggtga actcgttgat agggcttgcc
      901 aggtcgggat agcccagagc ggctccgatc accacgtcct tgttgtcggg gatgttgagc
      961 atgtccttga tatcatcctc ataggcgttg ataagcccga tggggcaggt ggaaaggccg
     1021 aagttgtggg cgatcaggac aaaatatccg agggcgatcc cgatgtccac gagacgggcc
     1081 tcggaaaagg cgttgtcgag gcaaaaaatg actgccgacg gcgcgccgta gaagttgcag
     1141 ctgccctcgt tgataaaccg gttgaactcc tggcccatct tctccaggta ggggttcatc
     1201 agtatgaagg attcagctcc cctcttgtga aactcagctg aaaggggctt cacgttcccg
     1261 ggactgcagg agatctgctt ttcacggtag agcttcacca gtctgcggct cagccgttcc
     1321 ttctcctcac ccatgacaac gatgaattcc cagggctgga gattgatggc cgacggcgcg
     1381 cggaccacca ggctcagtat ctcctctatc tgctgtctag agaccgcatc gggcttgaag
     1441 gcccgtgcgc tcttcctcac tttaatggct tcgatgagat ccatgggatc agtcctccta
     1501 gttgccgaac ccgccgacac tcataccgcg aattcggtct ctctttgtga tgccgtcact
     1561 gtcgcccgat gaacgatcct tccgtactca ttgacgagga tgctatcgac aaggtccttc
     1621 gctgccctct cgcagagcca gtcgggcagg cccgacatgg catggatcaa aagacacgcc
     1681 cttgtggtct cactctcgat cctgtccttg ttcgatcgct tccagagcca ccggcgggtc
     1741 agggcgtccc tgtcatcctt cacgatcacc tcacccttgt ctacggcctc ctgctcgccc
     1801 atatccatgg ggatgaaggt ttccgtgccg gcggcgaagg tgaggctgat atttccgtcc
     1861 accccgctga tagcgtgccc gctgatggga acaaggtact tgagggcgat cgaattgtac
     1921 aggtcgacta tggggttgaa acgcgggaac ggtcccccct tgagggcccg cttgagaagg
     1981 gattctataa aactcggata cttcgtcggg gccatcccca tcttcttgaa ggcttcccgc
     2041 caggctttta tattcggatg gtcctgcgga cgttcaaaag aaaaatggac tctcagatcg
     2101 tcaacga
//
LOCUS       DAOPOA010000441         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57098_length_2105_cov_3.280488, whole genome shotgun sequence.
ACCESSION   DAOPOA010000441 DAOPOA010000000
VERSION     DAOPOA010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57098_length_2105_cov_3.280488"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKJ17_08935"
     CDS             <1..197
                     /locus_tag="PKJ17_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76138.1"
                     /translation="YGATLLLQACLYVLWFAGSLNIPLSFLTRPSRAAHTFCAVNLAI
                     LIGWIKFLKGEKFTTWATQR"
     gene            204..1367
                     /locus_tag="PKJ17_08940"
     CDS             204..1367
                     /locus_tag="PKJ17_08940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOD76139.1"
                     /translation="MTHHSSKTNILFILQTFRTGGSERVVVDLCRRLDRGRFNCFAAA
                     LIDGDLRETLEGMGVPTVLPRIRSARQDALNVMREVSDFIGANAIRVVNAHHFTPFLY
                     GSYGARRHGCRTFYTVHSRREVEVMDRSWRVLGGVLVRLADGVIGISPDVSEAVKRQF
                     RLPDAKVFTLTNAVDHTRFAVDVDTRAKRRELGIGENDRVIGCVGNLRWDKNYPNLIR
                     AFGLLAERARDARLVIAGEGKRKDDLERLIAGTGLKGKVLLLGARDDVPEIMKTMDVY
                     CLSSFREGLPLSLLEAMSAGLPVVGTDVPGIRDVIVDGDTGLLVPSDDPARLSEALFK
                     VLADGELARGLSERGFRYVLREHGSGKWVAQYEALFSPNGRAGAGAGRVPLRV"
     gene            1372..>2105
                     /locus_tag="PKJ17_08945"
     CDS             1372..>2105
                     /locus_tag="PKJ17_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOD76140.1"
                     /translation="MEMSVQIVRNILSNWGSFAIGAAIQFLMMPFLVHRLGDTQYGIW
                     VLIISFTGFLGLFDFGVSGSVVKYVAEFRAKNDSEGLTRVCSSAYYVFLAAGVLAFAV
                     SIVMAFGFLHHFSIPREEISDARWVTLIIGLQIGLTLPLGFFTGYMRGIQRYDHVAGI
                     SLVILFARSLFIVVLVLAGYRLIAIALVHLVSTMAAGAIRAVYVFRADPALRLRPGLI
                     TRDALATVSRYSLLIFLYFVATRFIFS"
BASE COUNT          369 a          639 c          647 g          450 t
ORIGIN      
        1 tatatggagc gaccctcctc cttcaggcct gcctctatgt cctgtggttc gcggggtccc
       61 tcaacatccc cctctccttc ctcacgcgtc cgtcccgcgc ggcacatacc ttctgcgccg
      121 tgaacctcgc cattctcatc ggctggatca aattcctcaa gggagaaaaa ttcaccacct
      181 gggcaacgca acggtaaaga ccgatgaccc accattcctc taagaccaac atcctcttca
      241 tcctccagac cttccgcacc ggggggtcgg aacgggtcgt ggtggacctg tgccgtcgtc
      301 tcgaccgggg gaggttcaat tgcttcgcgg cagctctcat tgacggggac ctgcgcgaaa
      361 cactcgaggg gatgggtgtt cccacggtcc tgccgcggat acgatcggca cggcaggacg
      421 cgctgaacgt catgcgagag gtttcggact tcatcggggc caacgcgatc cgcgtggtga
      481 acgcccacca tttcacaccc ttcctctacg gttcttacgg ggcaaggcgt catggctgca
      541 ggaccttcta cacggtccac agccgcaggg aggtcgaagt gatggacagg tcctggcgtg
      601 ttctcggggg cgttctcgtg cgtctggccg atggggtcat agggatatcc cccgatgtca
      661 gcgaagccgt caaacggcaa ttccgcctgc ccgacgcgaa ggtgttcacc cttaccaatg
      721 ccgtcgacca cacccgtttc gcggtggatg tcgacacgag ggcgaagcgg agggaacttg
      781 gaatagggga gaacgaccgc gttatcgggt gcgtcggcaa cctcaggtgg gacaagaact
      841 atcccaacct cataagggcg ttcggtctgc tcgcggaacg cgcgcgcgac gcgagactgg
      901 tcattgccgg cgaagggaag aggaaggatg acctggaaag actcatcgcc gggacgggcc
      961 tcaagggaaa ggtgcttctc ctgggtgcca gggatgacgt cccggagatc atgaagacca
     1021 tggatgtcta ttgcctgagc tctttcaggg aaggactgcc tctttccctc ctcgaagcga
     1081 tgtccgccgg tcttcccgtg gtgggaacgg acgtcccggg tatacgggac gtcatcgtcg
     1141 acggcgatac gggtcttctc gtgccgtccg atgaccccgc aaggctctcg gaggccctct
     1201 tcaaggtgct cgccgacggg gagctcgcac gcgggctttc ggaaagaggt ttcaggtatg
     1261 tcctcaggga gcacggatcc gggaaatggg tcgcgcagta tgaggccctg ttctcaccga
     1321 acgggcgcgc gggtgcgggc gccgggcggg tcccgctcag ggtgtgatcg catggagatg
     1381 tctgtccaga tagtgagaaa tatcctgtcg aactgggggt cctttgccat aggcgccgcg
     1441 atccagttcc tcatgatgcc ctttctcgtc caccgtctcg gcgacacgca gtatgggatc
     1501 tgggtgctca tcataagctt cacaggcttt ctcggccttt tcgatttcgg ggtgagcggg
     1561 tccgtcgtga agtacgttgc ggaattccgg gcgaagaatg acagcgaggg tctgacaagg
     1621 gtgtgcagca gcgcgtacta tgtcttcctg gcggcggggg tgctggcctt cgcggtttcc
     1681 atcgtgatgg ccttcgggtt tctgcaccac ttcagcatcc cccgggagga gatctccgac
     1741 gcgcgatggg tcacgctcat catcggtctc cagatcggac tcacccttcc gctcgggttc
     1801 tttacgggct acatgagagg gatacagcga tacgaccacg ttgccggcat atcgctcgtc
     1861 atcctgtttg cccgcagtct cttcatcgtc gtcctggtcc tcgccgggta ccggcttatc
     1921 gccatagccc ttgtccatct cgtctcgacc atggcggccg gcgcgatacg ggcggtctat
     1981 gtcttcaggg ccgatcccgc tctcaggctc cgtcccggcc taatcaccag ggacgcgctg
     2041 gcgacggtaa gccgctacag tctgctcatc ttcctgtatt tcgtagcgac gaggttcatc
     2101 ttttc
//
LOCUS       DAOPOA010000442         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57101_length_2105_cov_2.937073, whole genome shotgun sequence.
ACCESSION   DAOPOA010000442 DAOPOA010000000
VERSION     DAOPOA010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57101_length_2105_cov_2.937073"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKJ17_08950"
     CDS             complement(<1..411)
                     /locus_tag="PKJ17_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76141.1"
                     /translation="MSEAVEVYEAHPMTLVGTPDSILAEATEAANALMTVVKKAGLAH
                     NFGGKKDHLEFEAWQTLAKFYGESVQCIEVKFVQYGSAQGFEAVSIVKDRFGDEVSRA
                     EAMCLNDEDKWKSKTKYAWAYVCKDGSRSVEDPGK"
     gene            complement(714..1076)
                     /locus_tag="PKJ17_08955"
     CDS             complement(714..1076)
                     /locus_tag="PKJ17_08955"
                     /inference="COORDINATES: protein motif:HMM:NF022476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76142.1"
                     /translation="MTCPQCGQEAKQVGKVFFCMTLDCPVCMVKGEEGEEGRGRMSHN
                     GIGSHQSARMMKSEWLTPPEIIQALGPFNLDPRAPFFDDKSKWIQDIRNLIARVEMAT
                     TERPASAQESGDIKGKEV"
     gene            complement(1076..1318)
                     /locus_tag="PKJ17_08960"
     CDS             complement(1076..1318)
                     /locus_tag="PKJ17_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76143.1"
                     /translation="MTEEARDILTRDLDKIAKKHGLTNCAFCGTTAEDEFYGAAVGDK
                     VSQTDVFNIAMNVGRLWQHFRGLTRDVLNTFEKKVW"
     gene            complement(1315..1722)
                     /locus_tag="PKJ17_08965"
     CDS             complement(1315..1722)
                     /locus_tag="PKJ17_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76144.1"
                     /translation="MQNSPAAERKPHRGDLNLLVLPGEQVRKPYSWRKYSSCPHCGST
                     GKWSQSAEMGHCSACGTWVPREGRAPWRQEPDYPYKTHKNPPKEIVCIDCGETFETHA
                     HGSKTPDRCEICRDRYHGKNKRERYRREREAQK"
     gene            complement(1712..1888)
                     /locus_tag="PKJ17_08970"
     CDS             complement(1712..1888)
                     /locus_tag="PKJ17_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76145.1"
                     /translation="MRNPEMSFRSFVYNLILIALMFVAFVYASKLGMEAAIEYADARE
                     AAMWSIAKGETHAK"
     gene            complement(1881..>2105)
                     /locus_tag="PKJ17_08975"
     CDS             complement(1881..>2105)
                     /locus_tag="PKJ17_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76146.1"
                     /translation="VPADENDVGCWMTNNREAIVKRSIEMQWELLDRLRKNPETMRAL
                     STILGAQVWLAANRDQVNEVIQNVTGGSHA"
BASE COUNT          361 a          643 c          535 g          566 t
ORIGIN      
        1 cttgccggga tcttcgacgc tgcgagatcc gtccttgcag acgtaggccc atgcgtactt
       61 tgttttgctc ttccacttgt cctcatcgtt gagacacatg gcctctgccc gcgaaacctc
      121 gtcgccgaaa cggtccttga caatggaaac ggcctcgaac ccctgggcgc tgccgtactg
      181 gacgaatttc acctcgatgc actggacgct ctccccgtag aactttgcca gggtctgcca
      241 tgcctcaaat tccaggtgat ctttctttcc gccgaaattg tgggcgagac cggccttttt
      301 tacgaccgtc atcagggcgt tggctgcttc cgtggcttct gcaagtatgc tgtcgggtgt
      361 gccgacgagc gtcattggat gcgcttcgta tacttccact gcttcactca tgattttcct
      421 cctctccaaa tatctccatt atggcgtcaa tcgccttcgc gtcggtcatc cgtccccatt
      481 catcggtcat catcagggca gccaaccgtt cccgtttgga acaaggtatg cacttcgtat
      541 cccccgccca tcggcactgt ccgtccttac agaactcgga aagggggtgc ggctctttat
      601 gctggcatga tgggctgtta cagtctgatg cgtggttgca gatcaaggtg tcctcctgtt
      661 tttaacgggg ccggatatgt tggcacatta cctccggccc ctctggggtg cggctatacc
      721 tcctttccct tgatgtcccc gctttcctgt gccgaggcag ggcgctcggt ggttgccatt
      781 tccactctgg cgatcaaatt cctgatgtcc tgtatccatt tgcttttgtc gtcaaagaag
      841 ggggcacggg ggtcaaggtt gaagggccct agagcttgaa taatctcggg cggtgtcagc
      901 cattcacttt tcatcatgcg ggctgactgg tgggaaccga tgccattatg gctcatcctt
      961 cctctccctt cctccccctc ttcccccttc accatgcaca ccgggcagtc cagcgtcatg
     1021 cagaagaaga ccttccctac ctgcttagct tcttgaccgc actgagggca ggtcatcacc
     1081 acaccttctt ttcaaatgta ttcagcacgt cccttgtcaa tcccctgaag tgctgccaga
     1141 gccgcccgac attcatggcg atattgaaaa cgtcggtctg cgagacctta tcgcctacgg
     1201 cggccccgta gaattcgtcc tctgccgtcg tcccgcaaaa agcgcaattc gtgagaccgt
     1261 gcttcttcgc tatcttgtca aggtctcgag tcaatatgtc ccttgcttct tccgtcattt
     1321 ctgcgcctcc ctctcccttc tgtacctttc cctcttgttc ttcccgtggt atctgtcccg
     1381 gcatatttcg caccgatcgg gtgtcttgct tccgtgggcg tgggtttcaa atgtctcccc
     1441 gcagtcgatg cagacaatct ccttcggcgg gttcttgtgc gtcttgtagg ggtagtcagg
     1501 ttcctgccgc cacggtgccc gtccttctct cggcacccaa gttccacacg ccgagcaatg
     1561 tcccatctcc gctgactgac tccacttacc cgtggagccg caatgaggac acgaggagta
     1621 tttgcgccac gagtagggtt tgcggacctg ttcgccgggg agtaccagga ggttcagatc
     1681 accgcgatgt ggtttgcgtt cggcggcggg gctattttgc atgtgtctct cctttggcta
     1741 tgctccacat ggcggcttcc cgtgcgtccg catactcaat ggcggcctcc atgccgagct
     1801 tcgaggcgta gacgaacgcg acgaacatca gggcgatcag gatgaggttg tagacaaagg
     1861 acctgaaact catttcgggg ttacgcatga gaacctcctg taacgttttg aataacttca
     1921 ttgacctgat cccggttagc cgcaagccag acctgagccc ccaatattgt tgataaggcc
     1981 cgcatggtct cggggttttt gcggagccgg tcgagcaatt cccactgcat ttcgatactc
     2041 cgttttacga tggcttccct gttgttcgtc atccagcaac cgacatcgtt ctcgtcggcg
     2101 gggac
//
LOCUS       DAOPOA010000443         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57136_length_2104_cov_2.924841, whole genome shotgun sequence.
ACCESSION   DAOPOA010000443 DAOPOA010000000
VERSION     DAOPOA010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57136_length_2104_cov_2.924841"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2074)
                     /locus_tag="PKJ17_08980"
     CDS             complement(<1..2074)
                     /locus_tag="PKJ17_08980"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOD76147.1"
                     /translation="MLLQCRQTFPPKAHVKIIWGRGVASKSGVATTKDQVLSYRARGP
                     FTARFRCMKEKPQGGCIPLSPMRLIFSAPISKKDAAKISIVMEKDAAKGKIWKPGIAE
                     DGESDITYLTFRGPFPEKTLLNVRIPPDLKDISGRSLSNADKFPLPARTDTYPALAKF
                     PDRFGVVEAGKEALLPVTVRNIEEEIETWLARGDEAGKPGRADRKDNAGQSVPKGQAG
                     ETGGAPPPGEMKGGIHKTAEDADASIIAWLHRLAASPRERSLLKGAPMSSAISIPKPG
                     GTKEFEVIGIPLSEPGFYVVEIESLMLGQRLLEKPGPVYVPTAALVTNMAAHFKWGRE
                     SSLVFVTALDSGEPVPNAAVTLRDCKGGLVWQGRADEKGIARIAKRLPNAESLPQCRR
                     PKEEKGLYYEYSRVLSGITGGLFVFARNGRDMTFTHSSWDDGIESWRFNLPGEYDDGR
                     GEPVIAHTVFDRTLLRAGETVHMKHFVRQRTMEGLFVPPDIRNISEVVIVHAGSEEKY
                     TLNLAWKGNGTAENTWKIPLAAKLGTYDVYLRSAPAAGKREVLGELSGSFRVEEFRVP
                     LMRGFIRGPKEPAIDARSVDLDIDVRYLSGGAAADHPVRIRSEMEQKHVSFDDYEDFT
                     FSRGRVKTGVEKLGETADRFAEDDALEDDESGEGSTRTRTDRFGRPRIRLKTLELNLD
                     RNGTARTTI"
BASE COUNT          362 a          681 c          618 g          443 t
ORIGIN      
        1 cgatggtcgt ccgcgcggta ccgttcctat cgaggttcag ctccagcgtt ttgagacgta
       61 tccggggccg cccgaacctg tcggtccttg tgcgcgtgga gccctccccg gactcatcat
      121 cctccaaagc gtcatcttca gcaaagcgat cagcggtctc gccaagcttt tcgacacccg
      181 tcttcacccg acccctggag aaggtgaaat cctcgtaatc gtcaaatgag acatgcttct
      241 gttccatttc agaacggatc ctgacgggat ggtccgcggc agccccgcct gagagatacc
      301 tcacgtcgat gtcgagatcg acggaccggg cgtcgatggc gggttccttc ggaccgcgga
      361 tgaagccgcg catgagaggg acccggaact cttcaaccct gaaggacccg gagagttccc
      421 ccaggacctc ccgcttgccg gcggcaggcg cgctgcggag atagacgtca taggtgccga
      481 gcttcgccgc gaggggtatc ttccacgtgt tctcggcggt gccgttgccc ttccatgcga
      541 ggttgagggt atacttctct tcgctccccg cgtgaacgat cacaacttcg ctgatattcc
      601 tgatgtcggg gggcacaaag agaccttcca tcgtgcgctg gcggacaaaa tgcttcatat
      661 gaaccgtctc tcccgcgcgc agaagggtcc tgtcgaagac ggtgtgggcg ataacgggct
      721 ccccgcggcc gtcgtcatac tcaccgggaa gattgaaccg ccaggactcg atgccgtcat
      781 cccagctgga atgggtgaag gtcatgtccc tgccgttgcg ggcgaacacg aaaagccccc
      841 cggtaatgcc cgacaacacc cgcgagtact cgtagtaaag ccccttctcc tccttggggc
      901 gccggcattg agggaggctc tccgcgttgg gaaggcgttt cgcgatcctt gcgatgccct
      961 tttcgtcggc cctgccctgc cagacgaggc cgcctttgca gtccctgagg gtgacggccg
     1021 cgttgggcac gggttcgccg ctgtcaaggg ccgtcacgaa caccagtgag gactcccggc
     1081 cccacttgaa atgagccgcc atgttcgtga ccagggcggc cgtgggcacg tataccgggc
     1141 ccggcttttc gaggagcctc tgcccgagca tgagactctc tatctcgacg acgtagaaac
     1201 cgggttcgga caggggtata cctatcacct cgaactcctt cgtccctccc ggtttgggta
     1261 tcgaaatggc gctgctcatc ggggcccctt tcaggagcga cctctcccgc ggagaggcgg
     1321 cgagcctgtg gagccatgct atgatcgacg catccgcgtc ttcagccgtc ttgtgaatgc
     1381 cgcccttcat ctcgccaggc gggggagcgc ctccggtctc acccgcctga cctttcggga
     1441 cagactggcc ggcattgtcc ttcctgtcgg cccggcccgg cttccccgct tcgtcccctc
     1501 tcgccagcca ggtctctatc tcctcctcga tgttgcgtac cgtaacgggc aggagggctt
     1561 ccttgcccgc ctcgacaact ccgaaacggt cgggaaactt ggcaagcgcg gggtatgtat
     1621 cggtcctggc gggaagagga aacttgtcag cgttcgacag ggaacggccc gagatatcct
     1681 tgaggtccgg cggaatgcgc acgttcagga gggtcttctc cggaaaaggc ccgcggaacg
     1741 taagatatgt gatgtcgctc tcaccatcct cggctatccc cggcttccag atctttcctt
     1801 ttgcggcgtc cttttccatc actatcgaga tcttagccgc gtccttcttc gatatcgggg
     1861 cagagaagat gagccgcatc ggcgacagcg gtatgcaccc tccctggggc ttttctttca
     1921 tgcaccgaaa ccttgcggtg aaaggcccgc gggcccggta ggagaggacc tggtcctttg
     1981 tcgtcgccac gccgcttttc gacgctacac ccctgcccca gatgatcttc acgtgggcct
     2041 tcggaggaaa cgtctgtctg cattgcagga gcaccgcact gtcctcttcc ttcttcgaga
     2101 cgcc
//
LOCUS       DAOPOA010000444         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57202_length_2102_cov_3.385442, whole genome shotgun sequence.
ACCESSION   DAOPOA010000444 DAOPOA010000000
VERSION     DAOPOA010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57202_length_2102_cov_3.385442"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PKJ17_08985"
     CDS             <1..506
                     /locus_tag="PKJ17_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOD76148.1"
                     /translation="REQGYSGAVDYGVIREVKEALDIPVIASGDNLSVDRVRRMFAET
                     GCDGVAIARGALGNPWIFRDVVRFFRDGTVPPGPDVAERVSVMKRHLELSVSHWGEKR
                     GVGIFHKFFIWYTRGFSGLRPLRDRAFRTGTMEGLIEVIDELTQRPERRGMFSARSRG
                     APIDPFV"
     gene            584..1522
                     /locus_tag="PKJ17_08990"
     CDS             584..1522
                     /locus_tag="PKJ17_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76149.1"
                     /translation="MARKRRCKIEDRGVRPVWTVLRTAFIIAAAFILSGCIDDTISVT
                     LRTDGSGTIEETVLMGNEFIEMMQGMGKGVGEEGKDRAGQTPVRGDAKNYDAVVTGMM
                     EKAKNNAKDFGEGVSLVSVKPARSDSASGYTAVYSFKDIGKVTLNQNPGSKTPLNDAD
                     KEKDKAEGAGSKDTPGKDRIRFVFTKGSPARLAVHMPPPKPADKDEKEKTAPKEPAKD
                     DPNAIEMMKAIFKGMRVRIALTVDGDIVKTNATHRLGRKITLIEMDFGRLISHAELLK
                     KIDREKPQSIEEMKAALNNVEGLKLEFEDPVVVDFK"
     gene            1564..1728
                     /locus_tag="PKJ17_08995"
     CDS             1564..1728
                     /locus_tag="PKJ17_08995"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HOD76150.1"
                     /translation="MKRTILLAGAFFLLAGCSTVYVKAGKSAEDFEKDREACEVVAKK
                     ELTARGAART"
BASE COUNT          496 a          540 c          702 g          364 t
ORIGIN      
        1 cgagggagca ggggtacagc ggggccgtcg actacggggt tatcagggag gtcaaggagg
       61 ctctcgacat ccccgtcata gcctcgggag acaacctttc cgtcgacagg gtccgcagga
      121 tgttcgccga gaccgggtgc gacggcgtgg ccatcgcgcg gggggccctc ggcaacccct
      181 ggatattcag ggatgtcgtg cgtttctttc gggacgggac ggtgccgccg ggaccggacg
      241 tggcggaacg tgtctccgtc atgaagcgcc acctcgaact cagcgtctcg cactggggcg
      301 aaaaaagggg cgtcggcatc ttccacaagt tcttcatctg gtacacgagg ggcttttcgg
      361 gcctgcgccc ccttcgggac agggccttca ggacgggcac gatggaaggc ctcatcgagg
      421 tcatcgacga gctcacgcaa agacccgaga gacgagggat gttttcggca cgcagccgcg
      481 gggcgcccat cgaccccttt gtctgaaatt gtcttgactg acctgtcaag gggttggtag
      541 cattgaccat gttttcctga gtcccgtccg cgagggagga ggaatggcgc gcaagaggcg
      601 gtgcaagatc gaagacaggg gggtccgtcc ggtatggacc gtgctaagga ccgccttcat
      661 catagcggcg gcattcatcc tttcgggttg tatcgacgat accatctccg tcacgctgag
      721 gaccgatggg agcggcacca tcgaagagac cgtcctcatg ggcaacgagt tcatagagat
      781 gatgcagggg atgggcaaag gtgtcggcga ggaaggcaag gacagggccg ggcagacccc
      841 cgtgcggggc gatgcgaaga actacgatgc cgtcgtgacg ggaatgatgg aaaaggcaaa
      901 gaacaacgcg aaggatttcg gggaaggggt tagcctcgtc tccgtcaagc cggcgagatc
      961 ggattcggcc agcggctata ccgcggtgta ctctttcaag gatatcggca aggtcacgct
     1021 gaaccagaac ccggggagca agacgcccct taacgacgcg gacaaggaga aagacaaggc
     1081 cgagggtgcc gggagcaagg atactcccgg caaggacagg attcgtttcg ttttcacgaa
     1141 agggtcgccc gccaggctcg ccgttcatat gcctcccccg aaaccggcgg acaaggacga
     1201 aaaggaaaag acggcgccga aggaaccggc aaaggatgac cccaatgcca tcgagatgat
     1261 gaaggccatc ttcaagggca tgagggtccg catcgccctg accgtggacg gcgatatcgt
     1321 gaagacgaac gccacccatc gtctggggcg gaagataacc ctcattgaga tggatttcgg
     1381 caggctcatc agccatgcgg aactcctcaa gaagatcgac agggagaaac cgcagtctat
     1441 cgaggagatg aaggcggcgc tgaataacgt cgaaggactc aagctggagt ttgaggatcc
     1501 tgtcgtcgtc gacttcaagt gacggacctt cataggaaca acaaactaag gagtcggaga
     1561 acaatgaaaa ggaccatatt gctcgcgggc gcgttcttcc ttcttgcagg gtgctcgacg
     1621 gtgtatgtga aggcggggaa gagcgccgag gatttcgaaa aagacaggga agcctgtgaa
     1681 gtcgtcgcga aaaaggagct cacggccagg ggcgccgccc ggacctgaag cgctgttttt
     1741 gaagagacaa ggcgttgtct cgaaaagaag ggttggagac cgaagggcta aggcgcatac
     1801 gggcagatgg gggaaaccgc gtctgcccaa aatagatttg cggcagcgga cagacagact
     1861 ctccgggaca tctcccggag ggggataatg acccgtgtga gcgtgtgccc atttccttga
     1921 ctttgcacag acaaaccatt aatttaaggt ttgattttca aagaatcgga caggaggctt
     1981 gaatcaatgg tcagggcaaa attgtggttc cggtgtgcgg cgatgcacga tcccgtgact
     2041 cccatggtcg cccagcccgc gcttgtcgga tgggaggcga agaggcgcag ggtggacctg
     2101 ac
//
LOCUS       DAOPOA010000445         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57246_length_2101_cov_2.856794, whole genome shotgun sequence.
ACCESSION   DAOPOA010000445 DAOPOA010000000
VERSION     DAOPOA010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57246_length_2101_cov_2.856794"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="PKJ17_09000"
     CDS             <1..1615
                     /locus_tag="PKJ17_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOD76151.1"
                     /translation="GFGLVTDASDIETISHIGLVLLLFLIGLEIDIKKLKESGRSLVV
                     TGLFQFILCVLLGLGFFYLLGFTVGGGRYDLVYLAACCALSSTAIVVKLLYGKFEIDT
                     LAGRITLGVLVFQDIWAIILLGIQPNLANPEILMILFSFLKGGVLVVFAFLLSKYLLP
                     RLFRKVSKTPELVLVASLGWCFFICGLAGYLGLSLEMGALIAGISISTFPYNLDVMAR
                     VINIRDFFVTLFFVALGMQIPNPMGSPGVLATAGIASAFLIVSRFLAVYPVLYSLRNG
                     NRVSLLTSINLSQISEFSLVITALGMRAGHIGQDVMSLVIFVFVITSVVSTYMINYSD
                     ALQKTLGFVLGRVGFGDIGSHDEGEREEAGREIMILGFHRNASSLISEIVEADGGVLS
                     EDTKNRIMVVDFNPEVHSSLRSLGIKVVYGDVGHLDTLHHAGLEKAKLVISTVPDSIL
                     VGTDNLTFIRHIRRINPGAKIIVTAESVRRALKMYAEGADYVFMPRILTARHLVTMMG
                     DILSADPARLEAIAREEMDALGRRHEIVG"
     gene            1642..>2101
                     /locus_tag="PKJ17_09005"
     CDS             1642..>2101
                     /locus_tag="PKJ17_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76152.1"
                     /translation="MNGKGGTMDAEKLNVGLFRPEDAKGVADLFTEVYGDAYPARIVY
                     DPKGLVEAFERRDQIPVVVRTGEGRVVGYSSLFRAAPDKGVYEKGNGAVSPAFRNAGI
                     MGMIFQYVGRTVPGIDGIKVFFGEPVCNHVYIQKAALANLPFVETALEVDL"
BASE COUNT          412 a          591 c          622 g          476 t
ORIGIN      
        1 cggttttgga ctggtcaccg acgcaagcga tatcgagacc atatcccaca tcggtctcgt
       61 cctccttctt ttcctcatag ggcttgagat cgacatcaag aagctgaagg aatcgggcag
      121 gtccctcgtt gtcacgggcc tctttcagtt catcctgtgc gttctgcttg gactcggctt
      181 cttctatctt cttggtttta ccgtcggtgg cgggagatat gacctcgtct accttgccgc
      241 gtgctgtgcc ctgagcagca cggccatcgt cgtgaagctg ctgtacggaa agttcgagat
      301 agacaccctg gcggggagga tcactctggg cgtcctcgta ttccaggaca tctgggccat
      361 cattctcctc ggtatccagc ccaacctcgc caatccagag atcctcatga tcctcttctc
      421 ctttctcaaa gggggcgtac tggttgtttt tgcctttctg ctcagcaagt atcttcttcc
      481 gcgcctgttc aggaaggtat ccaagacccc cgagcttgtc ctcgtggcgt ccctgggctg
      541 gtgtttcttc atatgcggtc ttgcggggta tctgggtctg tccctggaaa tgggcgccct
      601 catagcgggc atatcgatct cgacattccc ctacaacctc gatgtaatgg cgagggtcat
      661 caacatcagg gatttcttcg tgaccctctt cttcgtcgcc ctggggatgc agataccgaa
      721 cccaatggga agtcccgggg ttctggcaac agcgggcata gcatcggcgt tcctgatcgt
      781 ttcgcggttc ctggccgttt accccgtcct gtattctctc aggaacggga acagggtcag
      841 tcttctgacg tccatcaacc tttcacagat cagcgagttc tccctggtca taaccgctct
      901 gggaatgagg gccggccata tcggacagga tgtcatgtca ctggtcatct tcgtctttgt
      961 cataacctcc gttgtctcga cctacatgat caactacagc gacgccctgc agaaaaccct
     1021 cggctttgtc ctcggcaggg tgggattcgg ggacatcggc tcccatgatg agggagagcg
     1081 ggaggaggcc ggccgggaga taatgatact gggatttcac cggaacgcca gttcgctgat
     1141 aagcgagatc gtggaggccg atggcggggt gctctccgaa gacacgaaga acaggatcat
     1201 ggtcgtcgat ttcaaccccg aggtgcactc gtccctccga tcgctcggca tcaaggttgt
     1261 atacggtgac gtgggccacc tcgataccct ccatcacgcg ggtctggaga aggcaaagct
     1321 tgtcatctcg acggtgccgg acagcattct cgtcggtacg gacaacctca ccttcatccg
     1381 tcatatcagg aggataaacc ccggcgcaaa aatcatcgtt accgcggaaa gcgtccggag
     1441 ggcgctcaag atgtacgctg aaggggccga ttatgtcttc atgccgagga tactcacggc
     1501 acggcatctt gtgacgatga tgggagacat actgtcggcc gatccggcgc gcctcgaggc
     1561 gatagcccgg gaagagatgg atgcgctcgg ccgccgtcat gagatagtcg gctagaaggt
     1621 tcgacgccgg atggcgggta catgaatggt aagggaggaa ccatggatgc ggagaagctg
     1681 aacgtcggcc ttttcaggcc ggaagacgcg aagggggtgg cggacctctt caccgaagtc
     1741 tacggcgatg cctacccggc aaggatcgtc tatgacccca aagggcttgt cgaggctttc
     1801 gagaggcggg accagatacc ggtcgttgtc cggaccgggg agggacgggt cgtaggatac
     1861 agctccctgt tccgcgcggc gccggataag ggggtctatg agaagggtaa cggagcggta
     1921 tctcccgcct tcaggaacgc gggcatcatg gggatgatct tccaatatgt cggcaggacg
     1981 gttcccggca tcgacggtat caaggtgttc ttcggcgagc cggtctgcaa ccacgtgtac
     2041 atccagaagg ccgcgcttgc caatctgccc ttcgtcgaga cggccctcga agtggacctc
     2101 a
//
LOCUS       DAOPOA010000446         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57287_length_2100_cov_3.034719, whole genome shotgun sequence.
ACCESSION   DAOPOA010000446 DAOPOA010000000
VERSION     DAOPOA010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57287_length_2100_cov_3.034719"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PKJ17_09010"
     CDS             complement(<1..368)
                     /locus_tag="PKJ17_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOD76153.1"
                     /translation="MEGREVAPDSPYLTDNLKDYPVELNLLYENSTKRSCAAGEMIYI
                     QSETSRPEFYLIEKGRVKIALLSKDGSERTVIIQERNTLFGYAAAFDGHPHFQTATAL
                     EPTELRVIPVSTFLALNEKHP"
     gene            complement(772..1230)
                     /locus_tag="PKJ17_09015"
     CDS             complement(772..1230)
                     /locus_tag="PKJ17_09015"
                     /inference="COORDINATES: protein motif:HMM:NF025314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HOD76154.1"
                     /translation="MKKSYTILIATILAVTVLCVALDAEAVPNPRAIQWRASPRAFVT
                     SLYVGVLGRQPESQAVVNGWATQVNTTPHSRYQVFWRFVNSPEYQGSRWARQKREYNI
                     YRKYYMKSDSWEYSVSKGPLGADYYPHAGPYTFGVAMALRDYFETFYTRR"
     gene            1463..>2100
                     /locus_tag="PKJ17_09020"
     CDS             1463..>2100
                     /locus_tag="PKJ17_09020"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOD76155.1"
                     /translation="MIDRSDSTCFPLGPTVYGNGVNFSVFSKNADAVELLLFDHVDDG
                     TPARVIRLDPEEDRTYHYWHVFVPGLVPGQVYGWRVRGPSAPERGHRFDAGKVLLDPY
                     GKAVAVPHGYSRMAASQPGDNTAVAMKSVVADLDDYDWEGDGLLMRPFARTVIYEAHV
                     AGLTKNPNSGVAPEKRGTYAGLIEKIPYLKDLGITAIELMPVFQFDDQDAPPG"
BASE COUNT          398 a          614 c          633 g          455 t
ORIGIN      
        1 ggatgctttt cgttgagggc aaggaaggtg gaaacgggga tcacgcgcag ctccgtcggc
       61 tccagggcgg tggccgtctg gaaatgggga tggccgtcga aggcggcggc atagccgaaa
      121 agcgtgttac gctcctggat gatgacggtc ctttccgagc cgtccttgct gaggagcgct
      181 atcttgaccc tgcctttttc gatgagatag aattcgggcc ggctcgtctc actctgtatg
      241 tagatcatct cccccgcggc gcaagaccgt ttcgtcgaat tctcgtaaag caggttcagc
      301 tcgaccggat agtccttcag gttgtcggtc aggtaaggtg aatccggagc tacttctctc
      361 ccctccatgt gccccccctt gttttcggcc cctcttcttc caagtctatc ccctgtcggg
      421 gcccggtaat gttaacagct taacacagtc ggcgggctta ctgtataaac gggttagtgg
      481 aaagggtccg ggtcctccgt ctatgatcat ggagaacaaa ggaggtgagg catatgcatc
      541 atttcgacgt tatctccatt gtcctcggaa tcggcgtgat ggggatcccg ctcatgatcc
      601 tggcagtgag caaccggcag cggcgataac cttcgatcac gcggctgcag gcccccaacc
      661 ctcctgctgc accgacatgc ggcccggtcc cggagacgat tccggggcgc ggggccgcat
      721 gtgtccgcac gagactcgct tcgaggcggg tcgccgggcg ggaagacccg gttacctgcg
      781 ggtgtagaat gtttcgaagt aatctctgag cgccatggct accccgaagg tgtacggccc
      841 ggcgtgcgga taatagtccg cccccaacgg gcctttgctc acactgtatt cccaggaatc
      901 gctcttcatg tagtacttgc ggtaaatgtt gtattccctt ttctgccgcg cccaccggct
      961 tccctggtat tcgggcgaat tgacgaacct ccagaatacc tggtagcggg agtggggggt
     1021 ggtgttcacc tgcgtggccc acccgttcac cacagcctgg ctttcgggct gcctgccaag
     1081 gaccccgaca tagagggatg tcacaaaggc gcgcggggat gccctccact gtatggctct
     1141 tgggttcggg accgcttcgg catcgagagc gacgcaaaga acagtgacgg caagtatggt
     1201 tgcgatgagt atggtatagc ttttcttcat ttgccctcct ccagaatgat cgaaatatgg
     1261 gagccttcca ccatcatttc atggaaacca ggtacggcat acctgcatca tcagtttctg
     1321 tagcgttcct cgttgtggac gactccgggt aggctggctt aaaacaggat acccgccggg
     1381 aaaggcaatg tcaagggcgg ggcgctcgct ttcctttgac gacggtctga ataggtgcta
     1441 gcatagagac tgtcctggtg aaatgatcga ccgctccgac agcacctgtt ttcctcttgg
     1501 ccccaccgtg tacggtaacg gggtgaactt cagcgtcttt tcaaagaatg ccgacgcggt
     1561 ggagttgctt ctcttcgatc acgtcgacga cggaaccccg gcgcgcgtga tcaggctcga
     1621 ccccgaagag gaccgtacct accactactg gcacgtcttc gttcccggtc tcgtgccggg
     1681 gcaggtctac ggctggcgcg tccgcggccc ctccgcgccg gaaaggggcc ataggttcga
     1741 tgccggcaag gtccttctcg acccctacgg gaaggctgtg gccgtccctc acggatacag
     1801 ccgcatggcc gcctcccagc cgggcgacaa tacggcggtt gcgatgaaaa gcgttgtggc
     1861 cgatctcgat gactacgact gggaggggga cgggctcctc atgcgcccct tcgccagaac
     1921 ggtgatctac gaggcgcacg tggcgggact gacgaagaat cccaattccg gtgttgctcc
     1981 ggagaagaga ggcacctacg cggggctgat agagaagatc ccctatctga aggacctcgg
     2041 catcacggcc atcgaactca tgcccgtatt ccagtttgac gaccaggatg ctcccccggg
//
LOCUS       DAOPOA010000447         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57364_length_2098_cov_3.763093, whole genome shotgun sequence.
ACCESSION   DAOPOA010000447 DAOPOA010000000
VERSION     DAOPOA010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57364_length_2098_cov_3.763093"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PKJ17_09025"
     CDS             complement(<1..846)
                     /locus_tag="PKJ17_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD76156.1"
                     /translation="MNNAPGQNSAADNAVAVKGLTRVFGKFKAVDNIDLSVARGEIFG
                     FLGPNGAGKSTTIRMLCGLLLPTSGEGTVGGFDIMKQSEEIKKNIGYMSQKFSLYDDM
                     TIEENIDFFSGIYGVSEEMKKERKEWVLDMAGISHMRRTITRTMASGFKQRLALGCAV
                     IHDPPIIFLDEPTSGVDPISRRRFWNLIYDMSSKGTTVFVTTHYLDEAEYCDRLALIY
                     RGKIIAEGRPDTMKKEYMHREVLEISVSGIIEALDILGEYGFDAALFGSVIHATVDDA
                     ASAIPR"
     assembly_gap    954..963
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<964..1759)
                     /locus_tag="PKJ17_09030"
     CDS             complement(<964..1759)
                     /locus_tag="PKJ17_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD76157.1"
                     /translation="MNDAPVIRCTSLTKEFATVKALEDITLEVRKGELFGLVGPDGAG
                     KSTLMRLLAAVMEPTSGNAWVASHEIVKEPERIKEKIAYMPQRFGLYEDLTVMENIVF
                     YADIFSVPKDERPARIERLLGFSNLEPFTDRFAGQLSGGMKQKLGLACALIHTPEIVF
                     LDEPTNGVDPVSRRDFWKILYELLKERVTIFVSTSYLDEAERCTEIGLIYEGRLLEKD
                     TPGAIKARHAMPMIEVWSQNTRAIMQILKDHERVTGIGIYGDRLHIG"
     gene            complement(1763..>2098)
                     /locus_tag="PKJ17_09035"
     CDS             complement(1763..>2098)
                     /locus_tag="PKJ17_09035"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD76158.1"
                     /translation="KNVELGEIVQPGAAIFVVGDLEHPWVKVYVKEDKLSLVKLGQKA
                     RITVDTYKDRYYDGTVTFISSDAEFTPKNVQTQEERVKLVFGVKVTVKNKDQELKPGM
                     PADVRILLR"
BASE COUNT          398 a          600 c          576 g          514 t
ORIGIN      
        1 gcgggggatc gctgacgccg catcgtccac ggtcgcatgg atcacgctgc cgaaaagcgc
       61 ggcatcgaat ccgtactccc cgaggatgtc gagggcctcg atgatcccgg aaacggatat
      121 ctcgaggacc tccctgtgca tatactcttt cttcattgtg tcaggccgac cctctgcaat
      181 gatcttcccg cgatagatga gcgcgaggcg gtcgcaatac tctgcctcgt cgaggtagtg
      241 cgttgtaacg aagaccgttg tccccttcga cgacatgtcg tatataaggt tccagaacct
      301 gcgccgcgat atgggatcga cccccgatgt cggttcatcg agaaaaatga taggcgggtc
      361 gtgtataacg gcgcagccaa gagctaagcg ctgtttgaag ccgcttgcca tggtccgggt
      421 gatcgtgcgc cgcatgtggg agatgcccgc catgtcgagc acccactcct tgcgttcttt
      481 cttcatctct tcggataccc cgtaaatccc gctgaagaaa tcgatatttt cttcaatggt
      541 catatcatcg taaagcgaga atttttgcga catgtagccg atgttcttct taatctcctc
      601 ggattgcttc atgatatcga agccgcccac cgtgccttct cccgaggtag gaaggagcag
      661 gccgcacagc atccggatcg tcgtcgattt cccggcgccg ttcggcccca agaacccgaa
      721 gatctcgccg cgggccacgc tgagatcgat gttgtcgacg gccttgaact tcccgaatac
      781 ccgtgtcagg cccttgacgg cgactgcgtt atcggcggcc gaattttggc cgggtgcatt
      841 attcattgtt ttttacccga tgtgtttttt taccgatcat cgaaaagaaa atgtcctcta
      901 tcgatggcgc gatctctcga tactcctgta ccgcatggcc cttttccgac aacnnnnnnn
      961 nnntgccgat atggagcctg tcaccgtaaa tgccgatgcc ggtaacccgt tcgtgatcct
     1021 taagtatctg cataatggcg cgcgtgttct gcgaccatac ctcgatcatg ggcatggcgt
     1081 ggcgtgcctt gatcgcgccc ggggtatctt tttcgaggag cctgccctcg tatatcaggc
     1141 cgatctcggt gcatctttcg gcctcatcca ggtatgacgt ggaaacgaag atcgttaccc
     1201 tttccttgag gagttcgtac aggatcttcc agaagtccct gcgcgacacg gggtcgacgc
     1261 cgttggtcgg ctcatcgagg aaaactatct ccggggtgtg gataagggcg catgcgagac
     1321 cgagtttttg cttcatcccg ccggagagct gcccggcaaa cctgtccgtg aagggctcga
     1381 ggttcgagaa cccgaggagc ctctctatgc gtgccgggcg ttcatccttc ggcacgctga
     1441 agatgtccgc atagaaaacg atattctcca taacggtgag gtcctcgtag aggccgaacc
     1501 tctgtggcat gtaggcgatc ttttctttga tccgttccgg ttccttgact atttcgtgcg
     1561 aggccaccca ggcgttgcct gaggtgggtt ccataaccgc ggccaagagc cgcatgagtg
     1621 tcgatttccc ggcaccatcg gggcccacga gcccgaagag ctcacccttg cggacctcga
     1681 gcgtgatgtc ttcgagcgct ttgactgtcg caaattcctt tgtcagggat gtgcacctga
     1741 tgaccggcgc gtcattcatg gatcacctca ggaggattct gacatccgca ggcatgccgg
     1801 gcttgagctc ctgatccttg ttcttgacgg tgaccttgac gccgaagacg agtttgaccc
     1861 gttcctcctg cgtctggaca tttttcgggg tgaactcggc gtcggaggat atgaacgtca
     1921 cggtgccgtc atagtaccgg tccttgtatg tatccaccgt tatccgggcc ttctggccga
     1981 gcttgacgag gctcagcttg tcttccttga catatacctt cacccaggga tgttccaggt
     2041 cgccgacaac gaagatcgcc gcgccaggct ggacgatttc gccgagctcg acattttt
//
LOCUS       DAOPOA010000448         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57379_length_2098_cov_2.302986, whole genome shotgun sequence.
ACCESSION   DAOPOA010000448 DAOPOA010000000
VERSION     DAOPOA010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57379_length_2098_cov_2.302986"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PKJ17_09040"
     CDS             <1..697
                     /locus_tag="PKJ17_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOD76159.1"
                     /translation="GILVFANWGKPVDESGIWYTIHASKWYIAGLFALLFGYAIVRWF
                     GAKLIPTMITGVVVLILALLFPEWPLLAFSAGIAGVVWVTKISSDETRSWIDSTWMYA
                     LMIAPLLFGGVVVAGFLLGMPGTDNGIIPSRWVSAMVGGNGLFANLFGSIVGAFMYFA
                     TLTEVPIVQGLLGSGMGQGPALALLLAGPALSLPSMIVIRTVLGTKKTLVFVAYVIIL
                     STIAGMLYGWMVG"
     gene            760..990
                     /locus_tag="PKJ17_09045"
     CDS             760..990
                     /locus_tag="PKJ17_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOD76160.1"
                     /translation="MKKIEILGTGCAKCVKLAENAREAAQGAGIEFEIGKVTGINYIM
                     NYGVMVTPALVIDGVVKSVGKVLTVEEIKKLL"
     gene            1099..1236
                     /locus_tag="PKJ17_09050"
     CDS             1099..1236
                     /locus_tag="PKJ17_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76161.1"
                     /translation="MKRSFFIAVALITALALYGCATCPVPPSAPRTYSLNTSQGQEAR
                     K"
     gene            1249..1815
                     /locus_tag="PKJ17_09055"
     CDS             1249..1815
                     /locus_tag="PKJ17_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76162.1"
                     /translation="MGEATELNGARGGGSSVTDRMVRQRYLINIVLACLAIGLEIYYS
                     ICAGSCSYLRGTLLGVDLQYVGMAYMSCIILLSLLRKDTLLITLLSAGVGTEFYLIGF
                     QVWYDTYCPYCLAFAAILFVLFLLNFQRRHKMLCAVTMVLALIFFSLFFKGSLAPSYS
                     YTFNSLSPGDRTYPLQGVQRNDHHEETV"
     gene            1816..>2098
                     /locus_tag="PKJ17_09060"
     CDS             1816..>2098
                     /locus_tag="PKJ17_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOD76163.1"
                     /translation="MKMTLYRRMVENITRMLVLSCILLGLVYSIPVICRSAETFTPSF
                     GKGTVKVRLYSDYFCPPCRAMEPGIEPVITDLVRDGTITLTFVDTPFYRY"
BASE COUNT          424 a          595 c          575 g          504 t
ORIGIN      
        1 aggcatcctc gtcttcgcca actggggaaa accggtggat gaaagcggca tctggtacac
       61 catccatgcc tccaaatggt atatcgccgg cctcttcgct cttctcttcg ggtatgccat
      121 cgtcagatgg ttcggagcga agcttatccc gaccatgata acgggcgttg tcgttctcat
      181 cctggccctt ctcttcccag aatggccgct tcttgctttt tcggcgggta tcgccggtgt
      241 ggtgtgggtg acaaagatct cctccgacga gactcgaagc tggatagatt ccacgtggat
      301 gtacgccctc atgatcgccc ccctcctttt cggcggcgtt gttgtcgccg ggtttctcct
      361 cgggatgccg ggaacggaca acgggatcat accgtcccgg tgggtctccg cgatggtcgg
      421 aggcaacggt cttttcgcca accttttcgg gtccatcgtg ggtgcgttca tgtatttcgc
      481 gacactgacc gaggtaccca tagtgcaggg tctattgggc tcaggcatgg ggcagggccc
      541 cgccctcgcc ctcctccttg cgggaccggc attgtcgctg cccagcatga tagtcatcag
      601 aactgtcctc ggcacgaaaa agacccttgt attcgtcgcc tatgtgatca ttctttccac
      661 catcgcgggc atgctgtacg gatggatggt cgggtagcgt cgcaaggcgc gccatgcgtt
      721 acgcgcattg gaaaatgaac ccatcgggag gtgagcaaca tgaagaagat cgagatcctg
      781 ggaaccggat gcgccaagtg cgtgaagctt gccgaaaacg cccgggaggc ggcccaaggc
      841 gcgggcatcg agttcgagat cggcaaggtc accggtatca actacattat gaactacggc
      901 gtgatggtga cccccgccct cgtcatcgac ggtgtggtga aaagcgtcgg aaaggtcctt
      961 accgtggagg agatcaagaa actcttgtaa aggcacatga cttcagcagg cgctcccggg
     1021 agacggatat agccctgaaa gctgttctgg aaaaaatgtc gacttcgatg taagctatcc
     1081 gaactgttga gaggtaatat gaaacgatct ttctttatcg ctgtcgccct gatcaccgca
     1141 ctggccctgt acgggtgcgc cacatgccca gtgccgcctt cggcaccgag aacgtactcc
     1201 ctgaacacct cccagggtca ggaagcgcgc aagtaggatc gggtcggcat gggagaggct
     1261 acggaattga acggcgcgcg gggcggtgga tcgtccgtca cggacaggat ggtgcgtcag
     1321 agatatctca tcaatatcgt tctcgcctgt ctggccatcg gcctcgagat atactacagc
     1381 atctgcgcgg gctcatgctc ttacctccgg ggcacccttc tgggggtcga ccttcaatat
     1441 gtcggcatgg cctacatgtc ctgcatcatc ttattgagcc ttttgaggaa agatacgctt
     1501 cttatcaccc tgctgtccgc gggtgtggga accgaattct atctcatcgg cttccaggta
     1561 tggtacgata cctattgccc ctactgtctg gcattcgcgg cgatcctgtt cgtcctcttt
     1621 ttactgaact tccagcgacg ccacaagatg ctgtgcgccg tgacgatggt actggccctg
     1681 atctttttct ccctcttttt caagggatct ctcgcacctt cctactcgta cacattcaat
     1741 tctctttccc cgggggacag gacatacccc cttcaaggcg ttcagcgaaa tgaccaccat
     1801 gaggagacag tttagatgaa aatgacgctg tacaggagaa tggtcgaaaa tatcacgagg
     1861 atgcttgtcc tgtcttgcat tcttctcggc ctcgtatata gtatccctgt catctgccgg
     1921 tcagcggaga cattcacccc ttctttcggc aaggggacgg tgaaggtcag gctctactcc
     1981 gattatttct gtcccccctg cagggccatg gaacctggca tagaacccgt gataaccgat
     2041 ctggtacgag acggcaccat aactctcacc tttgtggaca cgccgttcta caggtatt
//
LOCUS       DAOPOA010000449         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57506_length_2095_cov_2.357843, whole genome shotgun sequence.
ACCESSION   DAOPOA010000449 DAOPOA010000000
VERSION     DAOPOA010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57506_length_2095_cov_2.357843"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..482)
                     /locus_tag="PKJ17_09065"
     CDS             complement(87..482)
                     /locus_tag="PKJ17_09065"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOD76164.1"
                     /translation="MAGVLVVGFLFIAVPAVVVVAVVWLLRKNQAGSRRLKAIPAGWR
                     PIEGRVIGNKVEEAARTNPGDWSFYFPMVLFEYTVEGQRYTGAQGVDRAYQEEYRVKK
                     VLAEYPVGNPITVYYDPIKPEDARLRMRS"
     gene            complement(563..805)
                     /locus_tag="PKJ17_09070"
     CDS             complement(563..805)
                     /locus_tag="PKJ17_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76165.1"
                     /translation="MVEEHERRVPAGGISGTRMGSRMGKAKKIVSAARAFRVVTTLVI
                     VVVPVAATVGAIVGYGAYSLVKQLKRRSSAVRPREG"
     gene            complement(869..1495)
                     /locus_tag="PKJ17_09075"
     CDS             complement(869..1495)
                     /locus_tag="PKJ17_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76166.1"
                     /translation="MMEGAERSLDYVPEDRRVAESLGWQPGIWASFFLLVGCIILAES
                     KSPPDLVMMEILGVVFFAGLVLALWQAVMHRNRTVLVRQDGMIQVFRKRRLDMVVAAQ
                     EIRLEKVDIVAIIKIGAPLAIAAALFLALGLAMLWKDRAANSGSLSALALGLVSTASL
                     ASAFWTRFFRRHLLVPVRNSRWLAEETVLLSVKQHKLLFSGEDGGRPL"
     gene            complement(1492..2058)
                     /locus_tag="PKJ17_09080"
     CDS             complement(1492..2058)
                     /locus_tag="PKJ17_09080"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOD76167.1"
                     /translation="MDKVEINSETILYPMPCSIVGTMVEKKPNYLAVGWFSMVNFKPP
                     CIAIALNKVHYSNGGIKESGAFSVNIPSVDMVEVTDYCGIVSGRKYDKSKEFATFYGK
                     SGAAPMITECPYNIECRLINVVDLPTNELFIGEVLAVYSDERYLTEGVPDMKKVNPLV
                     LSMPERSYLTLGGRVAAAWETGGRLVRK"
BASE COUNT          423 a          700 c          552 g          420 t
ORIGIN      
        1 gctgccgttc accgctatcg caagcatgaa tgacctccag tgtcgatatg aggacgcgct
       61 gaagacctac aggggaggcc tcgtcatcag gacctcatcc gcagcctggc atcctcgggc
      121 tttattggat cataatagac cgtgatcggg tttcccacgg gatactcggc aaggaccttc
      181 tttaccctgt actcctcctg gtatgcccgg tccacaccct gggcgccggt gtacctctgt
      241 ccctccacgg tatactcgaa gaggaccatg gggaagtaga agctccaatc cccggggttg
      301 gtacgggcgg cctcctccac cttgttccct atcaccctgc cctctatagg gcgccagccc
      361 gccggaatgg ccttcagcct ccggctcccc gcctggttct tgcgcaagag ccagaccacg
      421 gcgaccacga caacggcagg cactgcgatg aaaaggaatc ccaccaccaa aaccccggcc
      481 ataagccctc ctgcgaattg gtcaccgacc gatgcctgtg tattgcggga agatccgggt
      541 gacgtttgtg atccgtcctc gttcacccct cacggggacg aaccgcggac gatcttcgtt
      601 tcagctgctt caccagagag tacgcgccat agccgactat ggcccctacg gtggccgcca
      661 cggggacgac aacgatgacg agggtggtca ccaccctgaa cgcccgcgcg gcgctgacaa
      721 ttttcttcgc ctttcccatc ctcgacccca tgcgcgttcc cgatattccc cctgccggaa
      781 cccgtctttc gtgttcttca accattttcc tgcaatctcc cccgatttgc aagcaatatc
      841 aggaaccggc agcaagaccc ggcgatcatc acaagggcct gcccccgtct tctcccgaaa
      901 agaggagctt atgttgtttg acagagagaa gcacggtttc ctcggcaagc caccgggagt
      961 tcctgacggg caccagcagg tggcgtcgga agaaccttgt ccagaaagcg cttgccagcg
     1021 aagcagtcga aacgagccca agggcgaggg cgctcaagct gccagaattc gccgccctgt
     1081 ccttccagag catcgcgagc ccgagcgcca ggaacagagc ggcggcgatg gcgaggggcg
     1141 cgccgatctt gattatcgcg acgatgtcca ccttttcgag ccggatctcc tgtgcggcca
     1201 cgaccatatc gagacgtctc ttgcggaaga cctggatcat gccgtcctgc ctcacaagaa
     1261 cggtgcggtt cctgtgcatg accgcctgcc agagggccag cacgagcccg gcgaagaaaa
     1321 caacccccag gatctccatc atcacgaggt ccggaggcga cttggattcc gcgagaatga
     1381 tgcatccgac aaggagaaag aaagaggccc agatgcccgg ctgccaaccg aggctttccg
     1441 ccactcggcg gtcttcaggg acatagtcga gtgatcgctc cgccccttcc atcatttcct
     1501 taccaaccgt ccgcctgtct cccacgcggc tgcgacgcgc cctcccaggg tgagatagct
     1561 ccgctcgggc atggacagga caaggggatt caccttcttc atgtccggta ccccctccgt
     1621 caggtaccgc tcgtcgctgt acacggccag cacctcgccg atgaacagct cgttcgttgg
     1681 caggtccacc acattgatga gccggcactc tatgttgtag gggcattccg ttatcatcgg
     1741 cgccgccccc gacttgccat agaaagtcgc gaactccttc gacttgtcgt atttcctgcc
     1801 tgacacgata ccgcaatagt ccgtcacctc caccatgtcc acggacggga tgttaacgct
     1861 gaaagctccg ctctccttga tcccgccatt gctgtaatgg accttgttca gcgcgatcgc
     1921 gatgcagggc ggcttgaaat tgaccatgct gaaccatccc acggcgagat agttcggctt
     1981 cttctccacc atcgtgccga cgatggaaca gggcatgggg taaaggatcg tctcgctgtt
     2041 tatctccacc ttatccatca ggtccctcct catatcgcat ccgataccct accat
//
LOCUS       DAOPOA010000450         2093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57581_length_2093_cov_3.120707, whole genome shotgun sequence.
ACCESSION   DAOPOA010000450 DAOPOA010000000
VERSION     DAOPOA010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57581_length_2093_cov_3.120707"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKJ17_09085"
     CDS             <1..348
                     /locus_tag="PKJ17_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76168.1"
                     /translation="GKALHQVKSMTDDVKQSFEEVTTDDLEEPHKTKDAQATGTTGED
                     DAGGIGPEGADMPYAAETTATGEPDMIEFPAGQEADEEQQVQTAGAEQGLKESPQGQP
                     GTGRSGGQGNLRG"
     gene            398..1153
                     /gene="tatC"
                     /locus_tag="PKJ17_09090"
     CDS             398..1153
                     /gene="tatC"
                     /locus_tag="PKJ17_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOD76169.1"
                     /translation="MAKKINPDEKQPFVAHLKELRQRLVVCLLSVCVAFALTYFFRER
                     VNIFLLQPFKKIMPPGSSFIFTGVTEAFLTYFKIWILTAVTVASPVIIHQMWMFVSPG
                     LYEKERRYVYPLIFWGSLLFAGGVVFCYFIVMPNLYRFFVSYASDFVIPMPDLKGYVS
                     LTLKLLVIFGFLFELPLVAYFLAKVGILNHRFLAKKRRYAILGAFILSAIITPPDPIS
                     QILVAMPLWGLYELSIVIARLFGKKEKTANEGS"
     gene            1140..>2093
                     /locus_tag="PKJ17_09095"
     CDS             1140..>2093
                     /locus_tag="PKJ17_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOD76170.1"
                     /translation="MKDLDIVILAAGRGERMVSRKPKVMHEIMGKPLVGHVLDAARTL
                     EPARIVVVTGYGRHIVEAYLEDKGVLMAFQEEQKGTAHALASARGHLEGNDVLVLLGD
                     VPLIENFTLVRFLEFCRASASIVFLTTDVDDPSGYGRMVMDGDIIADIREEADATIEE
                     KLIRRINTGICYIPFRDLGLIDLIDTANRKNERYLTDICKVARTQGRHAKGFFHPRAE
                     EVLGVNTLKGLLEANAVMRQRINERHMARGVTFLDADIYVENDVVIGRDTVIAPHCHI
                     AGSTVIGEGVYIGPSSIIRNCRIGDNVTIAGLVFMEGVEAKV"
BASE COUNT          447 a          628 c          611 g          407 t
ORIGIN      
        1 ggtaaggccc tccatcaggt caaaagcatg accgacgatg tgaaacagtc attcgaagag
       61 gtcaccaccg acgatctcga agagccccat aagactaaag acgcacaagc caccgggaca
      121 accggggagg acgatgccgg cggcatcggg cccgagggtg cggacatgcc ttacgcggcc
      181 gagacgacgg ccaccggtga acccgatatg atcgagtttc ccgccggtca ggaggccgac
      241 gaagagcagc aggtgcagac agcgggagct gagcaaggcc tcaaggagag cccgcagggt
      301 cagccgggca caggcaggtc cggcggacag ggcaatttga gggggtaatt gcggacctga
      361 tcgcttcccg ggacgagaag acgatgtcag cctcgctatg gccaagaaga taaacccaga
      421 tgaaaagcaa ccctttgtcg cgcacctcaa ggagctgcgc cagcgcttgg tggtctgtct
      481 cctgtcggtc tgcgtcgcct tcgccctgac ctatttcttc cgggaaaggg tgaacatctt
      541 cctccttcaa cccttcaaga agatcatgcc cccgggcagc agtttcatct tcacaggcgt
      601 caccgaggcg tttctcacct atttcaaaat atggatactc acggccgtca ccgtggcatc
      661 gcccgtcatc attcaccaga tgtggatgtt cgtctctccc ggcctctacg agaaggaacg
      721 gagatacgtc tatcccctca tcttctgggg cagtctcctt ttcgccggcg gagtggtctt
      781 ctgttacttc atcgtcatgc ccaatctcta caggtttttc gtcagctacg cctccgactt
      841 cgttatcccc atgcctgacc tcaaggggta tgttagcctc acattgaagc ttctcgtcat
      901 cttcgggttc ctcttcgaac tcccccttgt ggcctatttt ctggcgaagg tggggatact
      961 gaaccatcgg ttcctggcaa agaagcggcg ctacgcgatc ctgggagcgt tcatcctgag
     1021 cgccatcata acacccccgg acccgatcag ccagatactc gtcgccatgc ccctctgggg
     1081 cctctacgaa ttgagcattg tcatcgcccg tttatttgga aagaaggaga agactgccaa
     1141 tgaaggatct tgatatcgtc attcttgccg cgggcagggg agaacggatg gtctcccgca
     1201 agccaaaggt catgcatgag atcatgggga aacccctcgt gggacacgtc ctcgacgccg
     1261 cgcgtactct cgaacccgcc cgcatcgtcg tcgtcaccgg gtatggacgt cacatcgtcg
     1321 aggcctatct cgaggacaag ggagtcctga tggcgttcca ggaagagcaa aagggaaccg
     1381 cccacgccct ggcatccgcg cgggggcacc tggaggggaa cgacgtcctc gttctcctcg
     1441 gtgacgttcc cctcatcgag aatttcacgc tcgtccggtt ccttgagttc tgccgggcct
     1501 cggcttcgat cgtcttcctg accacggatg tcgacgatcc ttccggctac ggccggatgg
     1561 tgatggatgg cgacatcata gcggatatcc gggaggaagc cgacgcgacg atcgaggaga
     1621 agctgatacg caggatcaac acgggcatct gttacatacc tttccgggac ctcgggttga
     1681 tcgacctcat cgataccgcg aacaggaaaa atgagcgcta cctgacggac atctgcaagg
     1741 tagccaggac ccagggccgc catgcgaagg gcttcttcca tccccgcgcc gaggaggtcc
     1801 tcggcgtcaa taccctgaag ggcctccttg aggcgaacgc ggtgatgagg cagcggatca
     1861 acgagcggca catggcccgc ggcgtgacgt tcctcgacgc cgacatctac gtggagaacg
     1921 acgtggtcat aggccgcgac accgtcatcg caccccactg ccacatcgcg gggagcacgg
     1981 tcatcgggga aggtgtctac atcgggccct catcgatcat caggaactgc aggataggcg
     2041 acaacgtgac catagcgggc ctggtcttca tggagggggt ggaggcaaag gtg
//
LOCUS       DAOPOA010000451         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57685_length_2090_cov_4.429975, whole genome shotgun sequence.
ACCESSION   DAOPOA010000451 DAOPOA010000000
VERSION     DAOPOA010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57685_length_2090_cov_4.429975"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKJ17_09100"
     CDS             complement(<1..514)
                     /locus_tag="PKJ17_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76171.1"
                     /translation="MTENTSAATYKRVFLVDGHSYLYRAFYATPHLSNSRGVPTNAIY
                     AFVSMIRKLASSENPDTLIVVFDSRGPSFREEISREYKAQRPAMPDNLSLQVPFVKQI
                     IDAMGLPVVEKEGFEADDIIATITEHLRTRDDVMTFIVTGDKDMMQLVGDRVRILDSM
                     KNVVIGEAEVI"
     gene            complement(534..1010)
                     /locus_tag="PKJ17_09105"
     CDS             complement(534..1010)
                     /locus_tag="PKJ17_09105"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOD76172.1"
                     /translation="MSKGPARKMDVIDKRILNVLQEEFPLTERPFETVAAKIGLTEEE
                     TRNRVKDLKDGGRIRRIGPVLDAKRLGYTSLLCGVAIPPDRLGQISGTVSEEPGVTHN
                     YEREGELNLWFTVTMERKEDIERFLSMLEETFSITIQRFPEKRTFKIRTRFPVTED"
     gene            complement(1007..1528)
                     /locus_tag="PKJ17_09110"
     CDS             complement(1007..1528)
                     /locus_tag="PKJ17_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOD76173.1"
                     /translation="MNDGSAQGSKEADLFLEELEVRSRESGYHLNPDTGFVRALIEGL
                     LTNVRRYGYAACPCRLASGRKEDDMDIICPCDYRDADLGEHGSCFCALYVSREVARGT
                     RPLASIVERRPPEGSRRHNDEPVAGIVRPSHPVWRCRVCGYLCAREGPPETCPICKAA
                     GERFELFLPGGPG"
     gene            complement(1525..1800)
                     /locus_tag="PKJ17_09115"
     CDS             complement(1525..1800)
                     /locus_tag="PKJ17_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOD76174.1"
                     /translation="MTHVDGKDKGALALYTLSTCVWCRMMKGFLAKMQIGYDYVDVDT
                     LAAEEKAQTIEELRRWNPKCSFPSLVVNGEKCVVGFNEETLKETLNL"
BASE COUNT          378 a          625 c          599 g          488 t
ORIGIN      
        1 cgatcacctc cgcctcgcct atgacgacgt tcttcatgga gtcgaggatg cgcaccctgt
       61 cacccacgag ctgcatcatg tccttgtcgc ccgtcacgat aaaggtcatc acgtcgtccc
      121 tcgttctcag gtgttccgtg atggtggcga tgatatcatc cgcctcgaag ccctctttct
      181 ccacgacggg cagccccatg gcatcgatga tctgtttgac gaacggcacc tggagggaaa
      241 gattgtcggg cattgccggc cgctgcgcct tgtattcccg ggatatctcc tccctgaagg
      301 aaggcccccg ggagtcgaag acgacgatga gggtatcggg gttctcgctg ctcgcgagct
      361 tccggatcat gctcacgaag gcataaatgg cgttcgtggg aaccccccgt gagttggaga
      421 ggtgcggtgt cgcgtagaag gcgcggtaca gataggaatg tccatcgacg aggaagacgc
      481 gcttgtatgt cgcggcggat gtgttctctg tcatgttccc ttcctggcgg tctttagtct
      541 tcggttacag ggaaacgggt ccttatcttg aaggtccgtt tttcagggaa gcgctggatg
      601 gtgatggaga aggtctcttc cagcatcgag aggaaccttt ctatgtcctc cttcctctcc
      661 atcgtaacgg tgaaccagag gttcagctcc ccttcacgtt cgtaattgtg ggtgaccccc
      721 ggctcttcgc tcacagtgcc ggagatctgc ccgagtctat caggaggaat agcgactccg
      781 cacaggaggc ttgtgtaccc caacctcttg gcgtcgagca ccggcccgat gcgccgtatg
      841 cggcccccgt ccttgaggtc cttcaccctg ttcctggtct cttcctcggt caggccgatc
      901 tttgctgcca cagtctcaaa aggcctttcg gtcaggggga actcttcctg cagcacgttg
      961 agtattctct tgtcgatgac gtccatcttc ctcgcggggc ccttgctcat ccagggcctc
     1021 ccgggagaaa aagctcgaag cgctcccctg cggccttgca gatggggcat gtctccgggg
     1081 ggccttccct ggcgcaaaga tacccgcaga ccctgcacct ccacacgggg tgggagggtc
     1141 ggacgatccc ggcaacgggc tcgtcgttgt gacggcggga accttccggc ggccttcttt
     1201 ccactatgga cgccaggggg cgggtccccc gggcaacctc ccgcgagaca tagagcgcgc
     1261 agaaacagga gccatgctcg ccgaggtccg cgtcccggta gtcgcaagga cagatgatgt
     1321 ccatatcgtc ctctttcctg cccgaggcaa gccggcaggg acaggccgcg taaccgtacc
     1381 tcctgacgtt tgtcagaagt ccctcgatca gggccctgac aaaaccggta tcggggttga
     1441 ggtggtatcc cgattcccgc gaccgaacct caagctcttc gaggaagaga tctgcctctt
     1501 tcgaaccttg cgccgatccg tcgttcataa attgagggtc tccttaaggg tctcctcgtt
     1561 gaacccgacg acgcacttct cgccattcac aacgagagaa gggaaggagc atttcgggtt
     1621 ccagcgcctg agctcttcga tcgtctgcgc cttctcttca gccgccaggg tgtcgacgtc
     1681 gacgtagtcg tagccgatct gcattttggc gagaaatccc ttcatcatcc tgcaccacac
     1741 gcaggtgctg agggtgtaga gggcgagagc gcccttgtcc ttgccgtcca catgggtcat
     1801 tcgcatctcc ggacctcctt tcgaacatag agtctctcca cgtccctatg gtatcacagc
     1861 gcccccggcg aaaaacaggt ttttcttttc ttccgaagaa tgacgggatg gaggtcaaaa
     1921 tgtgatatac tgtcgccatg ccagagaaaa aagggaggag ttccatgaga agagcctttg
     1981 ttctgtccat tcttctatca gtttgttttg ctgccttttc ttcgttcgcg gccggtgagg
     2041 cccgggcggc cgagcctttt gtctgtccct gtgccgtcaa tgtggaggtg
//
LOCUS       DAOPOA010000452         2084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57924_length_2084_cov_3.598324, whole genome shotgun sequence.
ACCESSION   DAOPOA010000452 DAOPOA010000000
VERSION     DAOPOA010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57924_length_2084_cov_3.598324"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKJ17_09120"
     CDS             complement(<1..670)
                     /locus_tag="PKJ17_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD76175.1"
                     /translation="MEPGEEMKEAGLENAEHVLVDLAKGRGIHLLEASTPVPGLKVNI
                     YFVERPVPTLIDAPMKGALCLAGLNGQLQPLGYSLDDIEVIIVTHPHIDHFGAAADIA
                     EMSGAQVWATENTARWLENYDDECLEEERFQKTFLDRAGVPADLVAHVGRYFQYLKKF
                     ACPVRVSRLLEVGEVVELASGRFRLEHVPGHTPWCVMMVDDREKIAFTGDFLLKDISS
                     NPLAQ"
     gene            760..1275
                     /locus_tag="PKJ17_09125"
     CDS             760..1275
                     /locus_tag="PKJ17_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HOD76176.1"
                     /translation="MGKAGWCAMLLAGLILCSCVTPVLQRSFMDRGKRNPDLAELVAN
                     PSGHLGQLFVFGGIIASTTVTREGSLLEAVYVPVNQYGNLGSEPTPSRRFLALYREGV
                     LDPVIYSPGRQVTLAGTFMETRPGKLGEMEYTFPFFQVTEIYLWPKEPKVYYYVPPPY
                     YSPYPWYRPWY"
     gene            complement(1303..1788)
                     /locus_tag="PKJ17_09130"
     CDS             complement(1303..1788)
                     /locus_tag="PKJ17_09130"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOD76177.1"
                     /translation="MMDGLLPGYKKCFFCGPATGGLGLELHYTDDGAICEFTAGDRFQ
                     GYDGMLHGGIVTGILDEVMWWTVFTRTKRMYATSRIEVEFKRPVECGKTYRASGSLLR
                     NTGRIYYLSGVIEDERGKICARGTASFREFRFTLEELARHLDFRGVSPEIRALFEPPA
                     S"
     gene            complement(1831..>2084)
                     /locus_tag="PKJ17_09135"
     CDS             complement(1831..>2084)
                     /locus_tag="PKJ17_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD76178.1"
                     /translation="REYDGDIDLLLTDVVMPGMNGRDLAEQIIGIRPDLKCLYMSGYA
                     VDVIAHKGILEEGVHFIAKPFSLYDLAGRVRTVLEPREQ"
BASE COUNT          424 a          639 c          575 g          446 t
ORIGIN      
        1 tctgggccag agggttcgac gatatgtcct tcagcaggaa atctcccgtg aaggctatct
       61 tctccctgtc gtcgaccatc atgacgcacc acggcgtgtg gccgggcacg tgctccagcc
      121 tgaagcggcc cgaggcaagc tcgacaacct ccccgacctc caggagacgg gacaccctga
      181 cggggcaggc gaacttcttc aggtactgga aataccgccc gacatgggca acaagatccg
      241 cgggcacgcc ggcacgatca aggaaggtct tctggaatcg ttcctcctcg aggcattcat
      301 cgtcgtaatt ctcgagccac cgggcggtat tctccgtggc ccatacctgc gccccggaca
      361 tctccgcaat gtccgcagcc gcaccgaaat ggtcgatatg aggatgtgtg acaatgatga
      421 cctcgatgtc atcgagggag taaccgaggg gttgaagctg tccgttcaac ccggcgaggc
      481 acaaagcgcc tttcatggga gcatcgatca gcgtcggcac gggtctctcc acgaaataga
      541 tgttgacctt caagccgggg accggtgttg acgcctcaag gagatggatc ccgcggccct
      601 tcgcaaggtc cacgagaaca tgttccgcat tttccagacc tgcctctttc atctcttcgc
      661 cgggctccat tataccacgc gatgcgatac ccgatgtcaa ggggattgcg caagcaaagt
      721 atctggctta ctctacatct ataggaaacg gaggtgaaca tgggaaaggc aggatggtgt
      781 gccatgttgc tggccggctt gatcctctgc tcctgcgtga cccctgtgct gcagcggtcc
      841 ttcatggacc ggggaaagag gaatccggac cttgccgaac tcgtcgccaa cccttcaggg
      901 catctcgggc agcttttcgt gttcggcggt atcatagcct ccacaacagt cacgagggaa
      961 ggctcccttc tcgaggcggt ctacgtgccc gtcaatcagt acgggaacct cggaagcgag
     1021 cctacccctt cacggaggtt cctggctctc tatcgtgaag gtgttctgga tcccgtcatt
     1081 tacagtccgg gcagacaggt cacccttgcg ggaacattta tggaaacacg cccgggaaag
     1141 ctcggggaaa tggagtatac ctttcccttc ttccaggtga ctgagatcta cctgtggccg
     1201 aaggaaccga aggtgtatta ttacgttccc ccgccctatt attccccgta cccctggtac
     1261 cgtccgtggt actgatggcc ccggcacggc agggggtgtg cctcaggagg caggcggctc
     1321 gaaaagagcc cgtatctcag gagagactcc cctgaagtcg aggtgcctgg cgagctcttc
     1381 cagggtgaat cggaactccc ggaaagaggc ggtgccgcga gcgcatattt tgccgcgttc
     1441 gtcttcgatg acgcccgaga ggtagtatat ccggcctgtg ttccgaagaa ggcttcccga
     1501 tgcgcggtac gtcttgccgc attcaacggg tctcttgaat tcaacttcga tcctggaggt
     1561 agcgtacatt cgctttgtcc gggtaaaaac ggtccaccac atgacctcgt cgagtattcc
     1621 cgtcacgatg ccgccgtgaa gcatcccgtc gtacccctgg aacctgtcac cggccgtgaa
     1681 ctcgcatatc gccccgtcat cggtatagtg caactccagg ccgagcccac cggtggcagg
     1741 accgcagaaa aaacatttct tgtaacccgg caaaagtccg tccatcattc attctccttc
     1801 atccattctc ccggtgcgtg cctctgcggc ctattgttcc cgcggctcca gcaccgtccg
     1861 gaccctgccg gcgagatcat acagggaaaa cggcttcgca atgaaatgga caccttcctc
     1921 caggatcccc ttgtgggcga tcacatccac cgcatatccg gacatgtaca ggcacttaag
     1981 gtccggtctt atgccgatga tctgttccgc gagatctctg ccgttcatcc ccggcatcac
     2041 cacgtcggtc agcaggaggt ctatgtcgcc gtcatactct ctgg
//
LOCUS       DAOPOA010000453         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_57955_length_2083_cov_3.896450, whole genome shotgun sequence.
ACCESSION   DAOPOA010000453 DAOPOA010000000
VERSION     DAOPOA010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57955_length_2083_cov_3.896450"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PKJ17_09140"
     CDS             complement(<1..236)
                     /locus_tag="PKJ17_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOD76179.1"
                     /translation="MSICCEGTAPKTPLLREIERRRTFAIISHPDAGKTTLTEKLLLF
                     GGAINMAGAVKARKAQRHATSDWMSIEQERGISVT"
     gene            complement(365..1417)
                     /locus_tag="PKJ17_09145"
     CDS             complement(365..1417)
                     /locus_tag="PKJ17_09145"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOD76180.1"
                     /translation="MDEAHRNGARLFMACRELGIDIRTRQRWLKDAAEGGVNTDGRKD
                     AERKKPVGALSEEERERVLEIADSPEYASKAPAQIVATLADKGEWVASESTFYRILHE
                     KNQQHIRGKAKSPNRKTPTSHAATAPNQVWTWDITYMGSPVRGKYYYLYMIVDIFSRY
                     IVGWEIHEEENAQYAKTLVQKAYLKEAIWKNGTPLVLHSDNGSPMKASTFRATLERLG
                     ITGSYSRPRVSDDNPYSESLFRTLKYRPEYPTYGFESLEASREWTYGFVHWYNEIHRH
                     SALKYVTPAQRHRGESAAILKARKSVLEEAKRKHPERWKGRAVRNLEEAQTVWLNPEK
                     DGRARETLTPEKRQVG"
     gene            complement(1453..1956)
                     /locus_tag="PKJ17_09150"
     CDS             complement(1453..1956)
                     /locus_tag="PKJ17_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOD76181.1"
                     /translation="MRMKRYSPEFKESVVRKMMPPENVPIMRLSEESGVSYVTLAKWR
                     DEVRGGGEAAPGNGERPDRWSSEDKFLIVLETHSLNEAELGEYCRSKGIFPAEIEEWR
                     ETCLMANQRDLRNRQEMQKEIRAEKKRSKELERELLRKERALAETAALLTLRKKAQAI
                     WGDDEED"
BASE COUNT          316 a          674 c          547 g          546 t
ORIGIN      
        1 gtcacggaga tgccgcgctc ctgctcgatg ctcatccagt cgctcgtggc gtggcgctgg
       61 gccttccggg ccttcacagc cccggccata ttgatggccc ctccgaagag gagcagcttc
      121 tcggtcagcg tggtcttccc cgcgtcgggg tggctgatga tggcgaacgt gcgccggcgc
      181 tcgatctccc gaagcaacgg cgtttttggt gcggtacctt cgcagcagat agacatgttt
      241 cgtgcatttc tccttcttga aacgggcatg aaagagccgg ggaaaacccc ggctgttctt
      301 ctggtgtcta ttgtacgcta ttgggtggat ttttcaaaag gggggaggga taaagcggtg
      361 tctgtcaacc gacctgtcgc ttttccgggg ttaaagtttc ccgcgctctt ccatccttct
      421 ccggattgag ccataccgtt tgtgcctctt ccaggtttct caccgcacgc cccttccatc
      481 gctccgggtg cttcctcttc gcttcctcca gaactgattt cctcgccttg agaatcgcgg
      541 ccgattctcc acggtgacgc tgggcgggcg tgacgtactt caacgcgctg tgacggtgga
      601 tctcgttgta ccaatggacg aagccgtacg tccattctct cgacgcttca agactctcga
      661 atccgtaggt cgggtattcg ggcctgtatt tgagcgtccg gaagagggac tcggagtacg
      721 ggttgtcgtc gctgactctc gggcggctgt acgaaccggt tatgccgagt ctttccagcg
      781 tggccctgaa cgtggaggcc ttcatggggc ttccgttgtc cgagtggagc accagcggcg
      841 tcccgttctt ccagatcgct tccttaagat acgctttctg taccagcgtc ttcgcgtact
      901 gcgcgttctc ctcctcgtga atctcccacc cgacgatgta gcggctgaag atgtccacga
      961 tcatgtagag gtagtagtat ttgccacgga ccggcgagcc catatacgtg atatcccaag
     1021 tccacacctg gttcggggct gtcgccgcat gcgacgtcgg agtcttcctg ttcggactct
     1081 tcgccttgcc tctgatgtgc tgctggttct tctcatggag tatccggtaa aacgtcgatt
     1141 ccgacgccac ccattcccct ttgtccgcga gcgtcgccac tatctgcgcg ggtgccttgc
     1201 tcgcatactc ggggctgtcg gcgatctcga gcactctttc ccgctcctct tcgctcaagg
     1261 ctcctacggg ctttttgcgc tccgcatcct ttcttccgtc cgtgttcacg ccgccttcgg
     1321 ccgcgtcctt gagccatctc tgacgggtgc ggatgtcgat tccaagctcc cgacaggcca
     1381 tgaagaggcg tgctccgttt cgatgcgctt cgtcgatcag ctctaccgct ctcaggcgat
     1441 ccgggccgct gatcagtctt cctcgtcgtc cccccagatc gcctgcgctt tttttcgcag
     1501 cgtcagcagc gccgccgtct cggccagcgc cctctccttc cgcagcagct ctctttcaag
     1561 ttccttgctg cgcttcttct cggcccggat ctctttttgc atctcttgac gattgcggag
     1621 atcccgttgg ttggccatca ggcacgtctc gcgccactct tctatctccg cggggaagat
     1681 ccccttggac cggcagtact cgcccagttc ggcctcgttg agagagtgcg tctccagaac
     1741 gatcaaaaac ttgtcctcgg aactccaacg gtcggggcgt tcgccgtttc cgggcgcggc
     1801 ttcgccgccg ccccgcactt cgtctctcca cttcgccaac gtcacgtacg agacgccgct
     1861 ctcttcggag agtctcatta tcggtacgtt ctccggcggc atcatcttcc gcacgaccga
     1921 ctccttaaat tcagggctgt atctcttcat cctcatcgca cctcccgagt gtcgactcac
     1981 acttctttaa cacctcggtt caagcgactt ctattatgac acagggggtt tcgaacctgc
     2041 cttctaaatc acttttcata tgtatcatcg tttccccaaa tca
//
LOCUS       DAOPOA010000454         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58010_length_2082_cov_2.325111, whole genome shotgun sequence.
ACCESSION   DAOPOA010000454 DAOPOA010000000
VERSION     DAOPOA010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58010_length_2082_cov_2.325111"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1129)
                     /locus_tag="PKJ17_09155"
     CDS             complement(107..1129)
                     /locus_tag="PKJ17_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76182.1"
                     /translation="MSTVLNISGDEHITIDTFQPGDALGVGDLFRKVYGEDYPVKLVY
                     DPEGLVKAFEKHENIPAVARSSKGIVVGYVSIFRSAPVETVYEGGQGLVLADYREHGT
                     FRELIRYYSEVLAPAYGADAVFGEAVCNHIYSQKAWAMHGAIETALEVDLMPGAVYAK
                     EGTTGRVATLHMTRMYRPMEHTVYAPRQYEEAMRFIYDGFTSGARVAKATDESAEREP
                     TSIESSHFEFAHVTRMTVRQAGQDFPAVFEEYERDALQKGMVVIQVWLKLSWPFIAGL
                     TDWLSGRGYFFGGILPRWFGEDGLLMQKIAVRPNWEGIMLYSDRAKDILAAVREDWQR
                     VTTQIP"
     gene            complement(1168..>2082)
                     /locus_tag="PKJ17_09160"
     CDS             complement(1168..>2082)
                     /locus_tag="PKJ17_09160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD76183.1"
                     /translation="PTLSIVIITKDTRELLEGLFDSIEKDTSLTSLGREIIVIDNAST
                     DGTEKFVKSACPHARYIRNDRNAGFAVAVNQGARAAAGRYILLLNSDTRLIEGEVARM
                     VATAEDIPDLGLMGPQLVYEDLSLQRSVAAVPGLTGEVIPGSGRVSQPASDGNTGPAR
                     DVESLIGAAIMIRKAAFDDLHGFDERFFFFLEETDFCVRARQKGYRVVFFPGARVIHL
                     QGRTVRKTWVKGRMEYNISLRKFMKKHHGRLYLTAFDAVRLAKSLAFVIAVPLSLFGS
                     RMRLRYSYYLNLVVWYMRACPDDAGLRG"
BASE COUNT          412 a          646 c          614 g          410 t
ORIGIN      
        1 cctcggtacg cgttttagca cgtacacacg atttccaatc gtgctccttc agccgctcgg
       61 acatctctcc ggtgacatct gttataccac acttcagaaa accacctcaa gggatttgcg
      121 ttgtcaccct ttgccagtcc tcgcgcacgg ccgcgagaat gtccttcgcc ctgtcggagt
      181 agagcattat cccctcccag ttcggacgga ctgcgatctt ctgcatgagg agcccgtctt
      241 cgccgaacca ccgggggaga atgcccccga agaagtatcc ccgcccgctg agccagtccg
      301 tcagaccggc gataaagggc caggagagct tgagccacac ctggatgacg accatgccct
      361 tctgaagggc atcgcgttca tactcctcga aaacggcggg gaagtcctgc ccggcctggc
      421 gcacggtcat gcgcgtgacg tgggcaaatt cgaaatggct gctttcgatg gatgtcggct
      481 ccctttccgc ggactcgtcc gtcgccttcg cgacacgggc accgctcgtg aacccgtcat
      541 agatgaaccg catggcctcc tcgtattgcc ggggcgcgta gacggtgtgt tccatgggcc
      601 ggtacatccg cgtcatgtgc agggtggcca cgcggcccgt tgtgccctcc ttcgcgtaga
      661 ccgcgccggg catgaggtcc acctcgaggg cggtctcgat ggccccgtgc attgcccagg
      721 ccttctggga atagatatgg ttgcaaacgg cctcgccgaa gacggcatcg gcaccgtacg
      781 caggcgccag tacctcggag taatagcgga tcagctctcg gaaggtgccg tgctcgcggt
      841 agtccgcaag gacgagaccc tgtccccctt cgtaaaccgt ttcgaccggg gcggacctga
      901 agatgctcac gtaccccacg acgatgccct tcgaagaacg ggcgacggcg gggatgtttt
      961 catgtttctc aaaggccttg acgagcccct cgggatcgta gacaagcttt accgggtaat
     1021 cctccccata gaccttgcgg aaaaggtccc ccacgcccaa ggcgtcaccg ggctgaaagg
     1081 tgtcaatggt gatatgttcg tctcccgaga tattgagtac ggtcgacatg cgttccttac
     1141 ctcctattcg tcccgcaccg gcgtccgtca gccgcgaagt cccgcgtcat cggggcaggc
     1201 gcgcatgtac cataccacaa gattgaggta ataggaatag cggagccgca tccgggagcc
     1261 gaaaagggaa agggggacag caatgacaaa ggccagggat tttgccagcc tgacggcatc
     1321 gaaggccgtg aggtacaggc gtccatgatg ctttttcatg aacttcctca gggagatatt
     1381 gtattccatg cgacccttca cccaggtctt cctgaccgtc cttccctgga ggtggatgac
     1441 ccgggccccg gggaagaaga ccacccgata acccttctgc ctcgcccgga cgcaaaagtc
     1501 cgtctcctcg agaaagaaga agaagcgctc gtcgaaacca tgaaggtcgt cgaaggcggc
     1561 cttccggatc atgatggcgg caccgataag cgattccacg tcccgggcgg gccccgtgtt
     1621 cccgtccgac gccggttgag agacgcggcc cgaaccgggg atcacctctc ctgtgaggcc
     1681 ggggacggct gccaccgacc tttgaaggct caggtcctcg taaacgagct gaggccccat
     1741 caatcccaga tccgggatgt cttcagcggt ggccaccatc cgcgccacct ctccttcgat
     1801 gaggcgggtg tcggagttga ggagaaggat atatctgccg gcggccgccc gcgccccctg
     1861 attcaccgca acagcgaatc ccgcgttgcg gtcgttgcgt atataccggg catggggaca
     1921 ggcgctctta acaaatttct ccgtgccgtc agtggacgcg ttgtcgatga cgatgatctc
     1981 cctgccgaga gacgtaagcg aggtgtcctt ttcgatggaa tcaaagagcc cctccaggag
     2041 ctccctggtg tctttcgtga taatgactat ggaaagggtg gg
//
LOCUS       DAOPOA010000455         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58038_length_2081_cov_2.228036, whole genome shotgun sequence.
ACCESSION   DAOPOA010000455 DAOPOA010000000
VERSION     DAOPOA010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58038_length_2081_cov_2.228036"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PKJ17_09165"
     CDS             complement(<1..437)
                     /locus_tag="PKJ17_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76184.1"
                     /translation="MATEKKDMKKEGTVKGYVKNNSGSIYGIELADGTQFFLSRSSKI
                     DLAKFPVGSYADITYGMFNNRNYINAIVPAQSPQEELAGEPLEDDFVPERQEEPEYAP
                     GPAAPSPATSAPAPATPPPAQQPATPAPTVPAARVPIKDLSMD"
     gene            complement(508..>2081)
                     /locus_tag="PKJ17_09170"
     CDS             complement(508..>2081)
                     /locus_tag="PKJ17_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76185.1"
                     /translation="RLSPLREQLRVMQEKLKERSVLLAEVNDLSRSLTGLKAQAAALR
                     KELASAVQIAELLHEVPCDGEEKQSSCKLLASGVEMAAKVPGMKDELEYLLDTIAMSE
                     EELKEKNELMASKYPEAAIPQSLLDDISRAQAAAARADLLAHAEGQLADLSAEAKTLE
                     QTLLDAQAAAAEESSSISSRLEEIKTARTDLVTRNNETKAKKTAMEQEISDISSRAET
                     LRTDKRLYEENERRKTVMNEQIVKMHAGIEKVTKDVRQWKLIQQGFGREGLIALELTI
                     AAPSVSDIANALLTEMGGRFAIRFDTLEPRMTRGKVTGYKESFTVKVFDAEKGIEKDL
                     DDLSGGERVWVDESIARGIGIYNSRNTRSNLGFLVSDEKDGALDKDKKLEYVAMKRKV
                     IELGGDRQEIFISHTPEVWDLADEVIVVGADGVRLRSGVSVEVDIPRETPQQETVQPA
                     LPSDTGAEVLEIPKKKTRRKKDKVPADAPASPGGNVTLFPDATAMDPPRPAPSDEPEA
                     APEEDAGFSYMSPGG"
BASE COUNT          343 a          623 c          612 g          503 t
ORIGIN      
        1 tcatccatgc tgagatcctt gatgggcacc ctggccgccg gtacggtggg tgccggtgtt
       61 gcgggctgct gagcaggagg aggtgttgcg ggcgccggtg cgcttgtggc gggagaagga
      121 gcagcggggc caggcgcata ttcgggttct tcctgcctct cggggacgaa gtcgtcttcc
      181 aggggttctc cggcgagctc ctcctgcggt gactgcgccg gcacgatggc gttgatgtag
      241 ttacggttat tgaacatgcc gtaggtgatg tcggcataag agccaacagg aaatttcgcg
      301 aggtcgatct tgctcgacct cgacaggaag aactgggtcc catccgcgag ttcgataccg
      361 tagatggagc cgctgttgtt cttcacatac cccttgaccg ttccttcttt cttcatgtct
      421 ttcttttctg ttgccatatt ggtgtttcct cctctcttgg gttcttaccg gggtaaatag
      481 cactatcgcg aaaaagggag gaggcttcta cccgcccgga gacatgtagg aaaaacccgc
      541 gtcttcctcg ggagccgctt caggctcatc ggaaggcgcg ggccgtgggg gatccatggc
      601 agttgcgtcg ggaaagaggg tgacgttccc acccggagac gccggggcat ctgcgggaac
      661 cttgtccttc tttctgcgcg tcttcttttt gggtatctca aggacctcag cacccgtgtc
      721 agacgggaga gcgggctgaa cggtctcctg ctgcggggtt tcccggggga tatcgacctc
      781 gacggacaca ccactccgca acctgacgcc gtcggcgccc actactatga cctcgtccgc
      841 cagatcccag acctcggggg tatgtgatat gaagatctcc tgccggtcgc ccccaagctc
      901 gatgaccttg cgcttcatgg cgacatattc gagcttcttg tccttgtcca gggccccgtc
      961 cttctcgtcc gaaacaagaa agccgaggtt ggacctggtg ttcctggaat tgtagatccc
     1021 tatgccgcgg gcgatggatt cgtcgaccca cacccgttct ccacccgaaa ggtcgtcaag
     1081 gtccttctcg atcccttttt cagcgtcgaa gaccttgacg gtgaaggatt ccttgtaacc
     1141 ggtcaccttg ccacgcgtca tcctgggttc gagggtgtca aaacggatgg cgaaccttcc
     1201 acccatctcc gtcaggaggg cattggctat gtcggaaacg gacggcgcgg cgatcgtcag
     1261 ctccagggct atcagacctt cacgtccgaa cccctgctgg atgagcttcc actggcgcac
     1321 gtccttcgtt accttttcta tgccggcgtg cattttgacg atctgctcgt tcatgacggt
     1381 cttgcggcgc tcgttctcct cgtagagcct cttatcggtc cgcagcgttt ctgcccggga
     1441 cgatatatcc gatatttcct gttccatggc cgtcttcttc gccttcgttt cgttgttccg
     1501 cgtaacgaga tctgtcctcg cggtcttgat ctcctcgagg cgggaagata tggaggagga
     1561 ttcctcggcc gcggcggcct gggcgtcgag aagcgtctgt tccagcgtct ttgcttccgc
     1621 cgagaggtcc gccagctgtc cttccgcgtg tgcgagcagg tcagcccgag ccgcggcggc
     1681 ctgggcccgc gatatgtcgt cgagaagcga ctgtggtatg gcggcctctg ggtacttcga
     1741 ggccataagc tcattctttt ctttcagctc ctcttcagac atggcaatgg tgtcgagcag
     1801 atactccagc tcatccttca tgccgggtac cttcgcggcc atctccacac ctgatgcgag
     1861 gagtttacag gaggactgct tttcctcccc gtcacagggg acctcgtgca ggagctccgc
     1921 tatctgcacg gccgatgcga gttccttgcg aagcgccgcg gcctgtgcct tgagccctgt
     1981 gagagacctt gagagatcgt tcacctcggc gagaagaacg ctcctctcct tcagtttctc
     2041 ctgcatgacc cgaagctgct ccctgagggg agacaaccgc t
//
LOCUS       DAOPOA010000456         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58211_length_2076_cov_2.835230, whole genome shotgun sequence.
ACCESSION   DAOPOA010000456 DAOPOA010000000
VERSION     DAOPOA010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58211_length_2076_cov_2.835230"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PKJ17_09175"
     CDS             <1..627
                     /locus_tag="PKJ17_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOD76186.1"
                     /translation="LVEESENNPVRDAKTMAAELQRSVFPEYRVALLHGRMSAKDKED
                     VMTRFRNGDIDVLVCTTVIEVGIDVPRATMIVMENAERFGLSQLHQLRGRVGRGSEPS
                     RCVLVASTKRTHLATRRLRIMEKTSDGFAIAEEDLRIRGVGDMLGVRQSGMPAFRIGD
                     IVRDIDIMIEARKRAEELTAGISEGEMARLMRHIGDRFDDGRDLSDIA"
     gene            655..>2076
                     /gene="pyk"
                     /locus_tag="PKJ17_09180"
     CDS             655..>2076
                     /gene="pyk"
                     /locus_tag="PKJ17_09180"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOD76187.1"
                     /translation="MRKARIVCTLGPPTQTARAIQSLVEKGMDVARLNFSHGDHVSHG
                     SLIRKIRAVEKKLRKPVAILQDLQGIKIRVGSLEGGKAALVKGQSVEIRAGSERGDGR
                     RVYIDYPWLIHDARPGDRILLDDGLIQLEVASTSGDSITATVVEGGVLKEHKGVNLPG
                     MKVSAAFFTEKDRADLEFGISMGVDYVALSFVRSADDVREVKTWMAARGVSVPLIAKI
                     EKEEAIRDIDGVLSEADGVMVARGDLGVEMPLEEVPIYQKMLIRKANEARKIVITATQ
                     MLESMTRHSRPTRAETTDVANAVLDGTDGLMLSAETSAGQYPFDSAEVMDRIISFTEE
                     HIRETGERFYVPYPEGSLFDFPGAVAEAASRAAHDTGARCIVAFTMTGFTALLISKFK
                     PDMPVVALSPDASVVRRMKLYRGVIPYQVRKLASTDRMIGEVDRFMVEAGFAKEGDVI
                     VLVAGHPIATKSRTNFMKLHRVGE"
BASE COUNT          458 a          561 c          698 g          359 t
ORIGIN      
        1 ctcgtggagg agtccgaaaa caatcccgtg cgggacgcaa agaccatggc cgcggagctg
       61 cagcgctccg ttttcccgga atacagggtg gcgctcctcc acggacgaat gagcgcgaag
      121 gacaaggagg atgtcatgac ccggttccgc aatggagaca tagacgtcct cgtgtgcacg
      181 acggtgatag aggtgggcat cgatgtgccc cgggccacga tgatcgtcat ggagaacgcg
      241 gagcgcttcg gcctctccca gctccaccag ctccggggca gggtgggcag gggatcggaa
      301 ccctcccggt gcgtcctcgt ggcgtcgacg aagaggaccc atctggcaac gaggcggctc
      361 aggatcatgg agaagacgag cgatggcttc gccattgccg aggaagacct caggatacgc
      421 ggtgtcggcg atatgctggg ggtcaggcag tcaggcatgc cggccttcag gatcggggac
      481 atcgtgagag atatcgacat catgatcgag gcccggaaga gggccgagga gttgacggcc
      541 ggcatttctg aaggtgagat ggcgaggctc atgaggcaca taggcgacag gttcgacgat
      601 gggagagacc tctcggacat cgcctgacga aaccgctcgg gaaaaggggg aactatgaga
      661 aaagccagga tagtttgcac gctcgggccg ccgacacaga cggcacgggc tattcagtct
      721 ctcgtggaga aaggtatgga tgttgcgcgc ctcaactttt cccatggcga tcacgtctcc
      781 cacgggtctc tcatcaggaa gatacgggca gtggagaaga aactccgcaa gcccgtcgcc
      841 atccttcagg acctgcaggg catcaagatc agggtagggt cccttgaggg gggcaaagcg
      901 gccctcgtga agggtcaatc cgtcgagata cgcgcgggct ccgagagagg agacgggcgg
      961 cgcgtctaca tcgactatcc ctggctcata catgacgccc gacccgggga caggatcctt
     1021 ctcgatgacg gcctgataca gctcgaggtg gcgagtacct cgggagattc catcaccgca
     1081 acggttgtgg aagggggggt gctgaaggag cacaagggtg tgaacctgcc cgggatgaag
     1141 gtgagcgccg ccttcttcac cgaaaaggac agggctgacc tcgagttcgg tatatccatg
     1201 ggcgtggact acgtcgccct ctctttcgtc aggagtgccg atgacgtgag ggaggtcaag
     1261 acctggatgg ccgcacgcgg ggtatcggta cccctcatag ccaagataga gaaggaagag
     1321 gcgataaggg acatcgacgg ggtactctcc gaggcggacg gtgtcatggt cgccagggga
     1381 gacctcggcg tcgagatgcc cctcgaagag gtccctattt accagaagat gctcatacgc
     1441 aaggccaacg aggcgaggaa gatcgtcata accgccactc agatgctcga atccatgacg
     1501 cgccattccc gtccgacccg ggcggagacg acggatgtgg ccaatgccgt gctcgacggc
     1561 acggacgggc tcatgctgtc cgccgagacg tcggccgggc aatacccatt cgattccgcg
     1621 gaggtaatgg accggatcat atccttcacg gaagagcata tcagggagac gggggaacgg
     1681 ttctacgttc catacccgga aggttctttg ttcgattttc ccggtgctgt cgcggaggca
     1741 gcttcgcgcg cggcacacga cacgggagcg cgatgcatcg tagccttcac gatgaccggt
     1801 ttcacggcac tgctcatctc gaagttcaaa cctgacatgc ccgtcgtggc gctttcgccc
     1861 gacgcttccg tggtgcggcg gatgaagctc tatcgcggcg tgatccccta ccaggtcagg
     1921 aaattggcaa gcactgacag gatgatcggg gaggtggaca ggttcatggt ggaggcgggt
     1981 ttcgcgaaag aaggggatgt catcgttctc gtggcaggcc acccgatagc cacgaaaagc
     2041 agaacgaact ttatgaaact gcacagggtg ggggaa
//
LOCUS       DAOPOA010000457         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58220_length_2076_cov_2.272637, whole genome shotgun sequence.
ACCESSION   DAOPOA010000457 DAOPOA010000000
VERSION     DAOPOA010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58220_length_2076_cov_2.272637"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PKJ17_09185"
     CDS             <1..388
                     /locus_tag="PKJ17_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOD76188.1"
                     /translation="PVGVYPLGFVFGYPFAIGSAMPYRPMTPEDIWVDDPDSPDYNRL
                     KKRGETAARSFEDMVLADDRYKYGIVIEYNTDPVIPGRGSAIFLHIRKDETTPTAGCV
                     AISEEDILTLIHWLDPAKKPLIVIEK"
     gene            complement(821..>2076)
                     /locus_tag="PKJ17_09190"
     CDS             complement(821..>2076)
                     /locus_tag="PKJ17_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOD76189.1"
                     /translation="LAFLWVLSRLRRRVNPRVVIWGLALQIVFALFVFLVPFGSRLFL
                     VLNDITMEVIESAFEGIRFLFGPLALSPGKAGSPGFILAFQALPSIIFFAALMELLYY
                     VGIMERLIRLFARVFVKVMGISGAESLCASAQIFLGIESNLTIRPYLKEMTPSELMVV
                     LTTGMATIASSVLGFYVIILSGALPKVAGHLISASILLAPAAILASKVLFPETGSPRT
                     LGKVVAVDYQKAANPIEAVINGAMTGVKMLVGIGALLIAFIGIVALFDSGLHYLGKLI
                     GSAAGTTVDLSLNHLLGYIFYPFTVAMGVPLDDAPQIARIIGERIIMTEVKSYQDLST
                     LVQAGKIVHERSVFIATYALCGFAHIPSLAIFVGGTSSLVPERTKDIAVLGLWALLAA
                     NVACFITGAVAGIFYTGTGGILFRP"
BASE COUNT          452 a          573 c          676 g          375 t
ORIGIN      
        1 acccgtgggc gtatatcccc tcggctttgt cttcggctac cccttcgcca tcggctccgc
       61 catgccctac cgcccgatga cgcccgagga catctgggtc gacgatcccg actcccccga
      121 ttacaaccgg ctcaagaaac gcggagaaac ggctgcccgc tccttcgagg acatggtcct
      181 tgccgatgac cgatacaagt acggcatcgt catcgaatac aacaccgacc ccgtcatccc
      241 cggccgcggc agcgccatct tcctccacat ccgcaaggac gagaccaccc ccaccgcagg
      301 ctgtgtggcc atctcggaag aagacatcct cacgctcatc cactggctcg acccggcaaa
      361 gaaaccactt atcgtcatcg aaaagtagaa agcaagaaac cttgcctctc gcccgttcgt
      421 cgcttgcgct cctcactcaa gcccgaaagg acgcagagaa aaggttcggc cggatttcga
      481 agcctcccaa aatccggccg gctttgcatc ccgtctccga cgggagggaa agaagaggtt
      541 tggtttcagg tgggagttgt gggggaggga tggaagtctg gggggttgga ggatggtcat
      601 aggacctatg ggacatatag gacctatgac gacaggggga ataacttctt gaggataaaa
      661 tagactattt ctttctctcc cgccgggacg gcgggacgag aaggccgccg gattttggga
      721 ggctccgaga tccggcggca atcttttctc tcttttcagt ctctctccgc gtcctctgcg
      781 aactcgagcg aagcgggcga gagataatgt ctttgatctt ttagggtctg aacaatatcc
      841 cgccggttcc ggtgtagaag atgcccgcca cggcgcccgt tatgaagcag gccacgttgg
      901 ccgcgaggag ggcccagagg ccgaggacgg ctatgtcctt cgtgcgttcg ggcacgaggc
      961 tcgacgttcc gccgacgaag atggcgagtg acgggatgtg ggcgaatccg cagagggcgt
     1021 aggtggcgat gaagacgctt ctttcgtgga ctatctttcc cgcctgcacg agggtgctga
     1081 ggtcctgata ggatttcacc tccgtcatga tgatgcgctc accgatgatg cgggctatct
     1141 gcggggcgtc gtcgagaggg acgcccattg ccacggtgaa gggatagaag atgtagccga
     1201 ggaggtggtt gagggacagg tcgacggtgg tgccggcagc ggaaccgatg agtttgccga
     1261 ggtagtggag gccgctgtcg aagagggcga cgatgccgat gaaggcgata aggagcgctc
     1321 cgatgccgac gagcatcttt acgccggtca tggcgccgtt gatgaccgcc tctattgggt
     1381 tagcggcctt ctggtaatcg acggcgacga cctttccgag tgttctgggg gagcccgttt
     1441 cgggaaagag gaccttcgac gcgagtatgg cggcgggagc gagcaggatc gaggcggaga
     1501 tgaggtggcc cgccaccttg ggcagggcgc cggaaaggat gatgacatag aagccgagaa
     1561 cgctcgacgc gatggtggcc atgcccgtcg taaggacaac catgagctcc gacggggtca
     1621 tctccttgag gtagggccgg atcgtcaggt tcgattcgat gccgaggaat atctgggccg
     1681 aggcacagag cgactcagcg ccgctgatgc ccatgacctt gacgaagacc ctggcgaaga
     1741 gtcggatgag gcgttccatt atccccacgt agtacaggag ctccatgagg gcggcgaaga
     1801 atatgatgga agggagtgcc tggaaggcga ggatgaaccc tggtgacccg gccttgccgg
     1861 gagacagcgc cagggggccg aagaggaacc tgatcccctc gaaggcgctt tcaatgacct
     1921 ccatggtgat gtcgttgagg acgagaaaga gcctcgaacc gaagggaacg aggaagacga
     1981 aaagggcgaa gacgatctgg agcgccagac cccagatgac cacccgggga ttcacccgcc
     2041 gcctgagcct tgagagaacc cacaggaacg cgagaa
//
LOCUS       DAOPOA010000458         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58366_length_2072_cov_2.917700, whole genome shotgun sequence.
ACCESSION   DAOPOA010000458 DAOPOA010000000
VERSION     DAOPOA010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58366_length_2072_cov_2.917700"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKJ17_09195"
     CDS             complement(<1..181)
                     /locus_tag="PKJ17_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOD76190.1"
                     /translation="MDKKYYITTPIYYINDVPHIGHAYTTIAADIMARYKRLGGYRVF
                     FLTGTDEHGQKVEKAA"
     gene            complement(333..1370)
                     /gene="ricT"
                     /locus_tag="PKJ17_09200"
     CDS             complement(333..1370)
                     /gene="ricT"
                     /locus_tag="PKJ17_09200"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOD76191.1"
                     /translation="MKSCYVNIDRLTGVLELQAVEDIKIGDFVISELDKGTCLGTVVS
                     EPVDTVREDLPKIGRKATEQEVRDHFQLKEKEKYAFDFCKRKIEDMGLPMKLLSTEYL
                     FGGTKLLFYFISENRVDFRELVKELAKEFKIRIELRQVGVRDEAKIVGGLGNCGNTVC
                     CRRFLNNFSIVSIKMVKEQGLALNPSKISGICGRLMCCLAYEYETYLSLKKNFPKIGK
                     RVKTPQGEGKVVKHNALGGTLSVQLDEGKEITLALKDISAAEQPRGAQPAAPVDKGKA
                     KTDNGRGQPKDRQQNPPDRGRQKNGGNREQTAHKQSNPGDRSIPRKNNGQKKDNGQTA
                     RPVANKEDTDQ"
     gene            complement(1374..>2072)
                     /locus_tag="PKJ17_09205"
     CDS             complement(1374..>2072)
                     /locus_tag="PKJ17_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76192.1"
                     /translation="DSRMIAKEVSEHPFEQDKRVIIIDNAETMTNEAANALLKTLEEP
                     PPYNHFFVITSSEREIPLTIRSRCTRVAFSPLSREQLELYFRDRAGIDPDRAGLLASI
                     SFGSIGSGLFWLDEDNFSLRVRMAEALSGRANGFVLASSLAEKASMTDRSASMYLTFL
                     LSFFRDLFVRKITGGTSFITNTDLEDILEGTSGDLPWIENSLKRIQETVRVMRYNVNR
                     WLVFENLLIHVARE"
BASE COUNT          381 a          575 c          585 g          531 t
ORIGIN      
        1 cggcggcctt ttccactttc tgtccgtgct cgtcggttcc ggtgagaaag aagacgcggt
       61 aaccgccgag cctcttgtag cgggccatga tatcggcagc tatcgttgtg tacgcatggc
      121 ctatgtgagg gacgtcgttg atgtaataga tgggggtcgt tatgtagtat tttttatcca
      181 tgacagatct ccgaggggga ataaccagca atcagactgc aataaccaca taaacttaca
      241 ataaccaatg accaaattac aataaccaat gagacttata atgaccggat tccagccgtt
      301 ccttgtctgg cgaaccggtc attggaatct ggtcactggt cggtatcttc cttgttggct
      361 accggtctgg ccgtctggcc gttgtctttc ttttgaccgt tgttctttct ggggatgctc
      421 cggtcgccgg ggttcgactg cttatgcgca gtctgctccc ggttgccgcc attcttttgc
      481 ctgccgcggt ccggagggtt ctgctgcctg tctttcggct gtcccctgcc gttgtcggtt
      541 ttcgccttgc ccttgtccac gggagcggcg ggttgggcgc ctctgggttg ttccgccgcc
      601 gagatgtctt tgagcgccag cgttatctcc ttgccctcgt cgagctggac ggacagggta
      661 ccgcccaggg cattgtgttt caccactttg ccctcgccct ggggcgtctt gacgcgcttg
      721 cctatcttgg ggaagttctt cttcagggac aggtacgtct cgtactcgta ggcgagacaa
      781 cacatgagcc tgccgcagat gccggatatc ttggaggggt tgagcgccag gccctgctcc
      841 ttcaccatct tgatggagac gatggagaaa ttgttgagga accgtctgca gcacacggtg
      901 ttgccgcagt tccccaggcc gccgactatc ttcgcctcgt cgcggacgcc cacctgccgg
      961 agctctatcc gtatcttgaa ctccttggca agctccttga ccagttcgcg gaaatccacc
     1021 ctgttctcgg agatgaaata gaaaagcagt tttgttccgc cgaagagata ttccgtggac
     1081 aggagcttca tgggaaggcc catgtcttcg atctttctct tgcagaagtc aaaggcgtac
     1141 ttctcttttt ccttcagctg gaagtgatct ctcacctcct gctccgtggc cttcctgccg
     1201 atcttcggaa ggtcttccct cacggtgtcc acgggctcgg acacgacggt accgagacag
     1261 gtgcccttgt cgagttcgct tatgacgaaa tcaccgatct ttatatcctc gacggcttga
     1321 agttcaagga cgcctgtcag cctgtcgata tttacataac aactcttcat tgcctattcc
     1381 ctcgcgacgt gaataagaag gttttcgaat accagccatc ggttaacatt atacctcatc
     1441 acgcgcacag tttcctgtat tcttttcagc gagttttcta tccaggggag atcgccgctg
     1501 gttccctcga ggatgtcctc gaggtcggtg ttcgtgatga acgacgtccc gccggtgatc
     1561 tttctcacga agaggtcacg gaagaaggaa aggaggaacg tcaggtacat ggaagcgctc
     1621 ctgtccgtca tggacgcctt ctctgccagg gatgacgcaa ggacgaaacc gttcgctctt
     1681 cccgacagcg cctctgccat cctgaccctc agtgagaagt tgtcctcatc gagccagaag
     1741 agcccgctgc cgatgctgcc aaaggagatg gaggccagaa gtcccgcccg gtcggggtcg
     1801 atgccggccc tgtccctgaa gtagagttcc agctgttccc tggaaagggg cgagaacgcg
     1861 acgcgggtgc accgggagcg gatggtgagc ggtatctctc gctcggagga ggtgatgacg
     1921 aagaagtggt tgtagggcgg gggctcctcc agggttttaa ggagggcgtt cgcggcctcg
     1981 ttcgtcattg tttccgcgtt gtcgatgata atgacccgtt tgtcctgttc gaaggggtgt
     2041 tccgaaacct ccttcgcgat catccgggag tc
//
LOCUS       DAOPOA010000459         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58374_length_2072_cov_2.607833, whole genome shotgun sequence.
ACCESSION   DAOPOA010000459 DAOPOA010000000
VERSION     DAOPOA010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58374_length_2072_cov_2.607833"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1473)
                     /locus_tag="PKJ17_09210"
     CDS             complement(142..1473)
                     /locus_tag="PKJ17_09210"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOD76193.1"
                     /translation="MDFVQTASLVIFLASIILVITGWIDSVLAALLGILFMVFFGIMS
                     DIDAFKIVDWNVIFILLSIWIISGYFGKSGVPDFLSAAILRLSRGNVALFVTMIGALA
                     GFVSMLIDNVVVVLMFAPVIFHACRRFGFPAFAPVLFLALCANFMGTAMLLGDLPPQM
                     LHSVSGIEFAGFIWQLGRPSSLFILLISYAVTCWVFYMMFKKKYRGITMDVASMNERP
                     LDHIKDKPFAITVCVVFALTILAMALRPIFGYHLGFIAMWGAICLILVFEMFKDRFTL
                     EIPSLEHVLSELDWRAVFFYVALFALVGGLEHSGVIKLVSNMITPLIKESLVVGSSLL
                     FWVTAPIVGIVEHDAYILTMLYVIRDLAKETGMNPWPLYWMLLWAGTLGSNLTIAGAP
                     ALFVAKNLGEKEDQVKVKLKEFLSMTVPFVIVSLIACYIPAMLIWVFPFAK"
     gene            complement(1759..>2072)
                     /locus_tag="PKJ17_09215"
     CDS             complement(1759..>2072)
                     /locus_tag="PKJ17_09215"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOD76194.1"
                     /translation="FVQPKTLIILILGLLAICLDTVCGVLFGKVLYVVTGGKVNPLIG
                     ASGISAFPMAARVAQKEGLKYNKRNYLLMHAMGANAGGQVGSVMAAAVMLSVLNGMGL
                     I"
BASE COUNT          538 a          557 c          580 g          397 t
ORIGIN      
        1 ggccctccga cgagatgtcc ttgaacattg tggacctgtc gatgtactcg tagcccaggt
       61 cccgggcgat cttcagggct attgccctgg ctccgccgcc gaactgccgt gaaattgtca
      121 atattgccat gatacacctc cctatttcgc gaacgggaag acccagataa gcatggccgg
      181 gatatagcag gcaatgagcg atactatcac gaagggaacg gtcatgctga gaaactcctt
      241 gagcttcacc ttcacctggt cctctttctc gccaaggttc ttcgcgacga agagcgcggg
      301 cgctccggcg atggtgaggt tgctgccgag agtaccggcc cacagaagca tccagtaaag
      361 gggccagggg ttcatgcccg tctccttcgc gaggtcacgg atgacgtaga gcatggtaag
      421 aatataggcg tcatgctcga caatacccac gataggagca gtgacccaga aaaggagact
      481 gcttcccacc acaaggctct ctttgatgag cggggttatc atgttggaca ccagcttgat
      541 gacgcccgaa tgctcgagac cgccgacaag ggcaaagaga gccacgtaga agaacacggc
      601 gcgccagtca agttcagaca ggacgtgctc caggctcggt atctccaggg tgaaccggtc
      661 tttgaacatc tcgaaaacga ggatgagaca gatggccccc cacatggcta tgaaaccgag
      721 atgatagccg aagatgggtc ttagcgccat ggccaggatc gtgagggcga acacgacgca
      781 cacggtgatc gcaaagggtt tgtccttgat atggtcaaga ggacgttcat tcatggatgc
      841 cacgtccatg gttatgcctc tgtatttctt cttgaacatc atgtagaaga cccagcaagt
      901 aaccgcatac gaaatgagaa ggataaataa cgaggacggc cttcccagct gccagatgaa
      961 acccgcgaac tcgatgccgg agacgctgtg gagcatctgg ggaggcaggt caccaagaag
     1021 cattgcagta cccatgaagt tcgcacagag ggcaaggaaa agcaccggcg caaaggcggg
     1081 gaaaccgaac ctccggcagg cgtgaaagat gacgggtgcg aacatgagga cgacgaccac
     1141 gttgtcgatc agcatggaga cgaatccggc cagcgcaccg atcatggtca caaagagggc
     1201 cacattcccc ctcgacaacc tgaggatagc cgccgagagg aaatcgggca cccccgactt
     1261 cccgaagtac ccggagatga tccagatgga cagcagaatg aagatgacgt tccagtcaac
     1321 gatcttgaag gcatcgatgt ccgacatgat cccgaagaag accatgaaga ggatacccag
     1381 caacgcggcc agaacgctgt cgatccaacc tgttatgacg aggatgatgc tggcgagaaa
     1441 gatcacaagt gatgcggtct gcacgaaatc catagactct gaaccccctt tttctgtgca
     1501 tcaccaagca gaccaagagc gtacactctt tagtcacccc gactagctga aggtctgcac
     1561 gattctagtt ttgatggaca ctgaagctac tatataacaa gtcacttctt tgaagcaagc
     1621 actatcgaat ggaagggtcg agttgcgggg cgagcccccc gactcctttt cctctttcca
     1681 gggataccta aaaaatgggg gggccggaca gatcgttgac tgtccggccc ctttccattc
     1741 cgttccctgt tattcttact agatgagacc cattccgttg aggacggaga gcatgacggc
     1801 ggcggccatg accgacccaa cctgcccgcc ggcattggct cccatggcat gcatgagaag
     1861 ataatttctc ttgttatatt tcaatccttc cttctgggcg acacgggccg ccatggggaa
     1921 ggccgatatg cccgaggcac cgatgagggg gttgaccttc cctccggtaa caacgtagag
     1981 gacctttcca aaaaggacgc cgcacaccgt gtcgagacat atggcgagaa gcccgaggat
     2041 aaggatgatg agggtcttgg gctgcacaaa ga
//
LOCUS       DAOPOA010000460         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58532_length_2068_cov_2.378539, whole genome shotgun sequence.
ACCESSION   DAOPOA010000460 DAOPOA010000000
VERSION     DAOPOA010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58532_length_2068_cov_2.378539"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKJ17_09220"
     CDS             <1..638
                     /locus_tag="PKJ17_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD76195.1"
                     /translation="KADLTAALAARPDYKAAQTKVKMLEERVASAQGKRLPDIYGAGD
                     YGGKAGDRMSFKENWSVGVRLFLPIFDFGRIGAEVDRERLELMKAKEEQRALRLAITR
                     ELKEAETSIADADERIAVSKKAVLSGKEQARIEDLRYRSGDNTSTDVINAEAALIRSR
                     ADHCQAIFDRRVAVVSLQKAMGTMDLPAPVAGEGPGVPGTPGGRPMTEEVK"
     assembly_gap    710..719
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <720..1627
                     /locus_tag="PKJ17_09225"
     CDS             <720..1627
                     /locus_tag="PKJ17_09225"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD76196.1"
                     /translation="NRNDGTMKLSGNVEATECNVGFKVAGKIATLTVDEGSRVKEGDL
                     IAELSSGDARTLVDQNKAALEEARVKLAELKAGSRRQEIVKARADSASLEAELVRARK
                     DFERAQTLYENGAISASRFDSAKSAYESRLGQLRSAKQQQSLIEEGPRREDIRAAELR
                     VKQLEALVANTEEKLADTRLYAPISGVVFRKNVELGEIVQPGAAVFTIGDLDRPWVKV
                     YVKEDKLALVKLGQKARITVDTYKDRAYDGTVTFISSGAEFTPKNVQTQEERVKLVFG
                     VKVTVTNKDQELKAGMPADVRISLR"
     gene            1624..>2068
                     /locus_tag="PKJ17_09230"
     CDS             1624..>2068
                     /locus_tag="PKJ17_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD76197.1"
                     /translation="MTTEDALAIRTESLSKSFGDVKALDGITLEVHKGELFGLVGPDG
                     AGKSTLMRLLAAVMEPSSGEAWVASYPVNKASERIKERIAYMPQRFGLYEDLTVMENM
                     TFYADIFDVPKAGRPARMDRLLGFSGLTPFTGRLAGQLSGGMKQKL"
BASE COUNT          452 a          627 c          668 g          311 t
ORIGIN      
        1 cgaaggccga cctcacggcc gccctcgccg cgcggccgga ctacaaggcg gcgcagacca
       61 aggtgaagat gctggaagag cgtgtagcct ccgcacaggg caagaggctg cccgatatct
      121 acggggccgg ggactatggt ggaaaggcgg gtgacaggat gtccttcaag gagaactgga
      181 gcgtcggcgt gcgtctcttt ctgccgatct tcgatttcgg gcgcatcggg gcggaggtcg
      241 accgggagcg cctggagctc atgaaggcaa aagaggagca aagggccctg cgcctcgcga
      301 taacccggga gctcaaggaa gccgaaacct cgatcgccga cgccgacgag cgcatcgcgg
      361 tttcgaaaaa ggccgtcctt tcgggaaagg aacaggctcg catcgaggat ctgcgctaca
      421 ggtcgggtga caacacgagc actgacgtca tcaatgccga ggccgccctc atccgctccc
      481 gggccgacca ctgccaggcc atcttcgacc gccgcgttgc cgttgtgtcc ctccagaagg
      541 caatgggaac aatggacctg cccgcaccgg tggcaggcga aggtcccggg gtacccggca
      601 cacctggcgg aagacccatg acggaggaag tgaaatgatc cccaagaaga gagtcattcc
      661 cctggcggtc attgtcgccg cggtcatcgt cgccgcggtg gagatcactn nnnnnnnnng
      721 caaccgcaat gacggcacga tgaaactttc cggcaacgtg gaggcgacgg aatgcaacgt
      781 gggcttcaag gtcgccggca agatagcgac cctcacggtc gatgaaggtt cccgcgtgaa
      841 ggaaggcgat cttatcgccg agctctcgag cggcgacgcg aggaccctcg tcgaccagaa
      901 caaagccgcg ctggaggagg caagggtgaa gcttgcggag ttgaaggcag gctcacgtcg
      961 ccaggagatc gtaaaggccc gggcggactc ggcctccctc gaggcggagc tcgtgagggc
     1021 tcgcaaggat ttcgagcgcg cccaaaccct ctatgagaac ggggccatat cggcatcgcg
     1081 cttcgattcg gcaaagagcg cctacgagtc gcgcctcgga cagttaagga gcgccaaaca
     1141 gcagcagagc ctcatcgagg aaggcccgcg cagggaggac atcagggccg ccgagcttcg
     1201 ggtgaagcag cttgaggccc tcgttgcgaa cacggaagag aaactggccg atacccggct
     1261 ctacgcgccc atatcggggg tcgtcttcag aaagaacgtc gagctcgggg agatcgtcca
     1321 gcccggcgcg gccgtgttca caatcggcga cctcgaccgc ccctgggtca aggtgtacgt
     1381 gaaggaggat aagctcgccc tcgtcaagct tggccagaaa gcgaggatca ccgtcgacac
     1441 atacaaggac cgggcctacg acggcacggt cacgttcatc tcctccggcg ccgaattcac
     1501 tccgaagaac gttcagacac aggaagagcg ggtcaagctc gtcttcgggg taaaggtcac
     1561 cgtcacgaac aaggaccagg agctgaaagc cggcatgccc gccgacgtga ggatctccct
     1621 cagatgacga cggaagatgc cctggcgata cgcacggaat ccctcagcaa gagcttcggg
     1681 gacgtaaagg ccctcgacgg gatcaccctc gaggtccaca agggtgagct cttcggcctc
     1741 gtcggccccg acggcgccgg gaaatcgact ctcatgagac tgctcgcggc ggtcatggaa
     1801 ccttcatccg gcgaagcctg ggtggcatcg tatcccgtaa acaaggcttc cgagaggatc
     1861 aaggaacgga tagcctacat gcctcagcgc ttcgggctct acgaagacct cacggtcatg
     1921 gagaacatga ccttttacgc cgacatcttc gacgtgccga aggccgggcg cccggcccgc
     1981 atggacaggc tcctcggctt ctccggtctt acccctttca ccggcaggct ggcaggacag
     2041 ctctcgggcg gcatgaagca gaagctcg
//
LOCUS       DAOPOA010000461         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58736_length_2062_cov_3.604385, whole genome shotgun sequence.
ACCESSION   DAOPOA010000461 DAOPOA010000000
VERSION     DAOPOA010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58736_length_2062_cov_3.604385"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2062
                     /gene="carB"
                     /locus_tag="PKJ17_09235"
     CDS             <1..>2062
                     /gene="carB"
                     /locus_tag="PKJ17_09235"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOD76198.1"
                     /translation="GDSITVAPAQTLTDKEYQVMRDASIAIIREIGVETGGSNIQFAV
                     NPADGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDEIPNDITKQTPAS
                     FEPTIDYCVVKIPRFTFEKFRGADETLTVSMKSVGEAMSIGRTFKEAFQKGLRSLEVG
                     RSGLVHDRLPGKDDLDLVRQKLITPNAERVFYVRHALRLGMDIEEIYAISHIDPWFLK
                     NIEEIVLFEKELEALKGKSPADMPAELLRRAKRLGFSDRQIGEITGAGEDGIRSLREK
                     MGLKSVFKLVDTCAAEFEAYTPYFYSTYEEEDESRVSDRKKIMILGGGPNRIGQGIEF
                     DYCCVHAAFALEELGYETIMVNSNPETVSTDYDTSDKLYFEPLTFENVLDIALSERPD
                     GVIVQFGGQTPLNLATALKNAGVPIIGTTPESIDIAEDRDKFKELLKDLGLTQPDNGI
                     ATSFEEARNIAASIGFPVVVRPSYVLGGRAMEIVYNDEDLASYMVKAVEASPEKPILI
                     DKFLEDAIEIDVDAVADGTRCVVAGIMEHIEEAGIHSGDSASALPPYSLNDDVIERIR
                     ICTYKLAGALNVVGLMNIQYAVKDDVIYVLEVNPRASRTVPFVSKATGVQWAKVAAKL
                     MAGKTLKDLGIEKEVVPQHMAVKESVFPFNKFAGVDTILGPEMKSTGEVMGIDSTFGS
                     AFWKS"
BASE COUNT          456 a          606 c          637 g          363 t
ORIGIN      
        1 ggggactcca tcaccgtcgc tcccgcccag accctcaccg acaaggaata ccaggtgatg
       61 cgtgacgcgt cgatcgcgat catccgcgag atcggtgtcg agacgggcgg ctccaacatc
      121 cagtttgccg tgaaccccgc cgacggaagg atggtcgtca tcgagatgaa tcccagggtg
      181 tcgaggagtt cggcgctcgc ctcgaaggcc acgggcttcc ccatcgccaa gatcgcggcg
      241 aagctcgccg tcggctatac cctcgacgag atccccaatg acatcacgaa gcagaccccg
      301 gcctccttcg agcccacgat agactattgt gtcgtgaaga taccgcggtt caccttcgag
      361 aaattcagag gtgccgacga gaccctcacg gtatcgatga agtccgtggg tgaggccatg
      421 tccataggaa ggaccttcaa ggaggccttt cagaagggcc tgaggtccct ggaggtcggc
      481 aggtcgggtc tcgtccacga ccggctgccc ggcaaggacg acctcgatct cgtgagacag
      541 aagctcatca cgccaaacgc ggagagggtc ttctacgtga ggcatgccct gcgtctcggc
      601 atggacatcg aggagatcta cgccatctcg cacatcgacc cctggttcct caagaacatc
      661 gaggagatcg tccttttcga aaaggaactg gaggctctga agggcaaaag cccggcggac
      721 atgccggcgg agcttctcag acgggcaaag agactgggtt tctccgacag gcagatcggg
      781 gagatcacgg gtgccgggga ggacgggata cggtcgctca gggagaagat gggactgaag
      841 agcgtcttca aacttgtcga tacctgtgcc gccgaattcg aggcatatac cccctatttc
      901 tattccacct acgaggaaga ggacgaatca cgcgtctccg acaggaagaa gatcatgatc
      961 ctgggcggcg gtcccaacag gatagggcag gggatagagt tcgactactg ctgcgtccac
     1021 gcggccttcg cccttgagga gctgggttac gagacgatca tggtcaactc gaaccccgag
     1081 acggtgagca ccgattacga tacctcggac aagctctact tcgaacccct caccttcgag
     1141 aacgtcctcg acatagcgct gagcgagcgt cccgacgggg tcatcgtgca gtttggcggc
     1201 cagacgcctc tcaacctggc gacggccctc aagaacgcgg gtgttcccat catcgggaca
     1261 acgcccgaga gcatcgatat cgccgaggac agggacaagt tcaaagagct tcttaaggac
     1321 ctcgggctca cccagcccga caacggcatc gcgacctcct tcgaagaggc gaggaacata
     1381 gcggcgtcga tcgggtttcc cgtggtggtg aggccgtctt acgtgctcgg gggaagggcc
     1441 atggagatcg tttacaacga cgaggacctg gcgtcataca tggtcaaggc cgtcgaggcc
     1501 tctcccgaga agcccatcct catagacaag ttcctcgagg acgccatcga gatcgatgtc
     1561 gatgccgtgg ccgacgggac ccgctgtgtg gtggccggga tcatggagca catcgaggag
     1621 gcgggcatcc attccgggga cagcgcgtcg gccctgcctc catactcgct caacgacgat
     1681 gtcatagaga ggatacggat ctgcacgtac aaactggccg gcgccctcaa cgtcgtcggc
     1741 ctcatgaaca ttcaatacgc cgtcaaggat gacgtgatct acgtcctcga ggtgaatccc
     1801 cgggcaagcc ggaccgtgcc cttcgtgagc aaggcgacgg gtgtccaatg ggccaaggtg
     1861 gcggcgaagc tcatggcagg caagacattg aaggaccttg ggatcgagaa agaggtggtc
     1921 ccgcagcata tggcggtgaa ggagtccgtc tttcccttca acaagttcgc cggtgtcgac
     1981 accatcctgg ggccggagat gaaatccacg ggagaggtca tgggcatcga ttccaccttc
     2041 gggtcggcct tctggaagtc cc
//
LOCUS       DAOPOA010000462         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58806_length_2060_cov_3.619451, whole genome shotgun sequence.
ACCESSION   DAOPOA010000462 DAOPOA010000000
VERSION     DAOPOA010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58806_length_2060_cov_3.619451"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PKJ17_09240"
     CDS             complement(<1..864)
                     /locus_tag="PKJ17_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76199.1"
                     /translation="MARVAIRGYSYCLNHTPELGLHYGVTPYIERITHGETEYIKKLC
                     KSMQSYEEACAYAPNQAYIGGMDLDDLKAQPAPMYGNRIDGASRYGKFGEIMPEKEFL
                     GLMDICDVFDIIWLEESFAGEVREVMARHPLMNEKILTRLEKGHASSEIEKETKEHGA
                     LPLYSQGRVAGCCRKGHDLDESLSAHVLLENIASKAGSVLALLHLLKTTGLSPADIDY
                     IIECSEEAAGDMNQRGGGNFAKAIAEIAGCVNASGFDIRSFCAGPVNAVIAGATQVAS
                     GARPNVVVLAGG"
     gene            complement(932..>2060)
                     /gene="grdB"
                     /locus_tag="PKJ17_09245"
     CDS             complement(932..>2060)
                     /gene="grdB"
                     /locus_tag="PKJ17_09245"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_except=(pos:complement(1193..1195),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HOD76200.1"
                     /translation="ADNLEKAEKEVLDLIASFSPDAVVAGPAFNAGRYGTACGAVCEA
                     VRKNLGLPTVSGMYEENPGVDMYRKSTYIARTADSARGMKDAVPAMARLLLKQLKGEP
                     IGKPDDEGYIERGVRVNFFMDKIGAERAVDMFVKKLKGEPFTTEYPMPTFDRVPPNPP
                     VADMKSATIALVTSGGIVPKGNPDHIEASSASKYGEYSLAGVMTINSSTHETAHGGYD
                     PTYANDDPNRVLPVDVMRDLEKEGAFKKFHETFYATVGNGTSVGNARRFGTEIAQKLK
                     DANVDAVIVTSTUGTCTRCGATIVKEIERAGLPVVHVCTIVPISLTVGANRIVPAVAI
                     PHPLGDPTKTPEEEKKIRRAIVEKALKALQTPVEDQTVFND"
BASE COUNT          364 a          625 c          597 g          474 t
ORIGIN      
        1 tccccccgcc aggaccacca cgttgggcct cgccccgctc gcgacctggg tcgccccggc
       61 tatcaccgcg ttgacaggtc ctgcacaaaa actcctgatg tcgaagcccg aggcattcac
      121 gcacccggcg atctccgcaa tggccttggc gaagttcccc ccgccccgct ggttcatgtc
      181 acccgcggct tcctcggagc attcgatgat gtagtcgata tcggccgggg aaaggccggt
      241 ggtctttaaa aggtggagaa gagccagcac cgatcctgcc ttgctggcaa tattttcaag
      301 aagaacgtgc gcggagagtg attcgtcgag atcatgcccc ttcctgcaac aaccggccac
      361 cctgccttgg gaataaagtg gaagggcacc gtgttctttc gtttccttct ctatctctga
      421 agaggcatga cctttttcca accgcgtcag gatcttctcg ttcatgagcg ggtgcctggc
      481 catgacttcg cggacttcac cggcgaagga ctcttcgagc cagataatat cgaagacatc
      541 gcagatatcc atcaagccga ggaactcctt ctcgggcatg atctcgccga atttgccgta
      601 ccgcgaggcg ccatcgatcc tgttaccgta catcggggcg ggctgcgcct tcaggtcatc
      661 caggtccatc ccccctatat aggcttggtt gggggcgtag gcacatgctt cttcatagga
      721 ctgcatgctt ttacacagtt tttttatgta ctccgtctct ccgtgggtga tcctctcaat
      781 gtacggggtg accccgtagt gaagccccag ttccggggta tggtttaggc aataggaata
      841 acccctgata gcgacacggg ccatgggccg ctcctccctc cgtttctata gacggatggc
      901 ccggataagg atccgggcca tccctgggta cttaatcgtt aaaaaccgtc tggtcctcta
      961 cgggcgtctg gagagccttg agggctttct ccacgatggc acgcctgatc ttcttctcct
     1021 cctcgggggt cttcgtcggg tcccccaggg ggtgagggat ggccaccgca ggcacgatcc
     1081 tgttcgctcc aacggtgagt gaaatgggca ctatggtgca gacgtggacg acgggaagcc
     1141 ccgccctttc gatctctttg acgatcgttg cgccgcaacg agtgcaggtc cctcaggtgg
     1201 aggtcactat cacggcgtcc acgttggcgt ccttgagctt ctgggcgatc tccgtcccaa
     1261 aacgcctggc gttgcccacg gaggtgccgt tgccgacggt cgcatagaaa gtctcgtgaa
     1321 atttcttgaa ggcaccctct ttttcaaggt cccgcatcac gtcgacgggc aggaccctgt
     1381 tgggatcgtc gttcgcgtag gtggggtcgt agccgccatg ggccgtctca tgggtcgaag
     1441 agtttattgt catgacgccg gccaggctgt attctccgta cttcgacgcg ctcgatgcct
     1501 cgatgtggtc ggggttgccc ttcggtacga tgccgccgga ggtcacgagg gcgatggtcg
     1561 cgctcttcat gtcagccacc ggcgggttgg gaggcaccct gtcaaaggtc ggcatgggat
     1621 attccgttgt gaagggctct cccttgagct tcttcacgaa catatccacg gccctctcgg
     1681 ccccgatctt gtccatgaag aagttcaccc ttacgcctct ttcgatgtag ccttcatcat
     1741 cgggcttgcc tatcggctcg cccttgagtt gcttgagaag aaggcgggcc atggcaggaa
     1801 ccgcatcctt catgcccctg gcgctgtcag ctgttctggc gatgtaggtc gacttacggt
     1861 acatgtcaac gccggggttc tcctcgtaca tacccgacac ggtcggtaga ccgaggttct
     1921 tcctgaccgc ttcgcagacg gcgccgcagg cggtgccgta acggccggcg ttgaaggccg
     1981 gcccggcgac gaccgcgtcg ggagaaaagg acgcgatcag gtccaggacc tccttctcag
     2041 ccttttcaag gttatcggcg
//
LOCUS       DAOPOA010000463         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58816_length_2060_cov_2.907731, whole genome shotgun sequence.
ACCESSION   DAOPOA010000463 DAOPOA010000000
VERSION     DAOPOA010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58816_length_2060_cov_2.907731"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PKJ17_09250"
     CDS             <1..343
                     /locus_tag="PKJ17_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOD76201.1"
                     /translation="VLLAELLKNLIDNAIKYTPSGGSVDVRLSRDGSNRIALTVSDSG
                     MGISPEDLPRIFDRFYRGDRSRSEAGLGLGLSLARAIARAHGGDITVTSIPDRGATFT
                     VTLPKKTAETA"
     gene            514..2004
                     /locus_tag="PKJ17_09255"
     CDS             514..2004
                     /locus_tag="PKJ17_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOD76202.1"
                     /translation="MERTRVIRAVVIVLATIALTVGLGYGVSKAVKGTNNTPVPSKSS
                     AVPIMVPGNFAELAEKARPSVVNIQTVKTLKGNGRVFRHFFGNPFGKESPFGGQDPFE
                     EFFGRRGGNGSPEDFRQKSLGSGFIVDGDGYIVTNNHVIDGANEIKVKLTDGREFDAS
                     IVGRDSKTDLALIRITGASNLTPITMGNSDELKVGSWVVAIGSPFGLEQTVTAGIVSA
                     KGRIIGSGPYDNFIQTDASINPGNSGGPLINMKGEVVGINTAIVASGQGIGFAIPVDT
                     ARNVIAQLKGNGRVTRGWLGVSIQEITPALASSFGLADRKGALVADVIKDGPADKAGI
                     AQGDVIVEFDGKDIATSNDLPRVVAATPVGKDVSVKLLRGGKTVSKTVKIARMEDTVA
                     EVAGKESGENKAGIAVQAITPEIARALQLKTRQGVVVTSVEPGSPAEEAGIIRGDVIR
                     EVNRTAVKDVEGFVRKMAEESGKKSVLFLIHRQGNNIYVVVKLARG"
BASE COUNT          481 a          576 c          629 g          374 t
ORIGIN      
        1 ggtgctcctc gccgaactcc tcaagaacct catcgacaac gccatcaaat acaccccctc
       61 cggcgggagc gtcgatgtcc gcctttcgcg ggacggctcg aaccgcatcg cgcttactgt
      121 tagtgacagc ggcatgggta tctcgccgga agaccttccc cgcatcttcg acaggttcta
      181 ccgcggcgac aggagccgct cggaagcggg cctgggcctg ggcctcagcc tcgcgcgggc
      241 cattgcccgt gctcacggtg gagacatcac cgtgacaagt atccccgaca gaggtgccac
      301 cttcaccgta accctcccca agaagacggc cgaaaccgct tgaaccgctt gagggccaaa
      361 gagacccgtc cttcccatga tccataaccc ataacctatt acccgtcttc attaccaatt
      421 tgtaatctca ccgtcatgtc ctgatgaact tcgcgatgta gactggagta tcggcaaggc
      481 agcgcctggc caatatacat caaggagacc tgtatggaac gaacacgagt aatcagagct
      541 gttgttatcg tactggcgac catcgccctt acggtggggc tcggctatgg ggtttcgaag
      601 gctgtcaagg gaacgaacaa cacgccggtg ccttcgaaga gctccgcggt gcccatcatg
      661 gtccccggca atttcgcgga actcgcggag aaggcccgtc cgagtgtcgt gaacattcag
      721 acggtcaaga cgctcaaagg aaacggacgc gtttttcgcc atttctttgg caaccccttc
      781 ggcaaggaaa gccccttcgg cgggcaggac cctttcgaag agttcttcgg ccgccgggga
      841 gggaacgggt cacccgagga tttccggcag aaaagccttg gctcgggatt cattgtcgac
      901 ggggacggtt atatcgtgac caataaccac gtgattgacg gtgccaacga gataaaggtg
      961 aaactaactg acgggagaga gttcgacgcg agcatcgtcg gcagggattc caagaccgac
     1021 cttgccctca tcaggatcac gggcgcttcg aacctcacgc cgataacgat gggcaattcg
     1081 gacgaactca aggtcggcag ctgggtcgtt gcgataggca gccccttcgg tctcgaacag
     1141 acggtgacgg cagggatcgt cagcgccaag ggcaggatca tcggctcagg gccttatgac
     1201 aacttcattc agacggatgc ctccataaat cccggaaaca gcggtggtcc tctcattaat
     1261 atgaaagggg aggtggtcgg aatcaacacg gcgatcgtgg cctcaggaca ggggataggc
     1321 tttgcgattc ccgtcgatac ggccaggaac gtgatcgcgc agttgaaagg taatgggagg
     1381 gtcacccgcg gctggctcgg cgtgagcata caggagatca ccccagcact cgcctcctct
     1441 ttcgggcttg cagacagaaa aggggccctc gtggccgacg tgatcaagga tgggcctgcc
     1501 gataaagccg gaatagctca gggcgacgtg atcgtggaat ttgacggtaa ggatattgcc
     1561 acctccaacg atctcccgcg ggtcgtagcg gcaacgcctg tgggcaagga tgtgtcggtg
     1621 aaactgctcc gcggcgggaa gacggtaagc aagaccgtca agatagcgcg gatggaagac
     1681 accgtggcgg aggtagccgg aaaggaatcc ggagagaaca aagcagggat agcggttcag
     1741 gccatcactc ccgagatagc aagggccctc cagctgaaga cccggcaagg tgttgttgtg
     1801 acgtccgtcg aaccggggag tccggcggaa gaagcgggca tcatcagggg cgatgtcatc
     1861 cgggaagtga acagaactgc cgtaaaggac gtggaaggct ttgtcaggaa gatggctgag
     1921 gagagcggaa agaaaagcgt tctctttctc atccacagac aagggaataa catctacgtt
     1981 gtcgtaaagc tcgccagggg ctaaaagagg cccctttccc ctgataccgg gctgccgggg
     2041 tccttcgggg accccggcag
//
LOCUS       DAOPOA010000464         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58827_length_2060_cov_2.267830, whole genome shotgun sequence.
ACCESSION   DAOPOA010000464 DAOPOA010000000
VERSION     DAOPOA010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58827_length_2060_cov_2.267830"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKJ17_09260"
     CDS             complement(<1..409)
                     /locus_tag="PKJ17_09260"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76203.1"
                     /translation="MIADIVIIALIKALQCALMALPESARQGTGRLLGRMGFHLLRRR
                     RNIAISNIRRAFPDLSPNKAGDIALSCFQKLGTNVIELLLVPFLPPREVPERFSLEPK
                     DIVERTLGAGRGVIALTFHFGNWEIMGITSKLLN"
     gene            complement(416..601)
                     /locus_tag="PKJ17_09265"
     CDS             complement(416..601)
                     /locus_tag="PKJ17_09265"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOD76204.1"
                     /translation="MTISQDLLDILCCPKCKGDLVLTASGNGLTCAACRLLYPIRDGI
                     PVMLIEEALPIKPDDPK"
     gene            complement(620..>2060)
                     /locus_tag="PKJ17_09270"
     CDS             complement(620..>2060)
                     /locus_tag="PKJ17_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOD76205.1"
                     /translation="LRLSKRRVDEIKTWEKIDKSLEDGTPLEGHILNEVKGGFIVDMG
                     INAFLPLSQVDIKPVKNPSSFVGRNLKFKVIKSNRRKGSIILSRRVLLEDERDKKRQE
                     FWKTVKDGQIMYGFVRNITDYGAFVDLGGVDGFLHINDITWGKITHPKEYLRIGDEVK
                     VKIIAIDTEKGRVSIGMKQLKTDPWLKIDEKYPVGSKVKGKVVGIVEYGAFVELEQGL
                     EGLLHVSEMSWDKKFKNPAKLVNKGDNLELVVLKIDLEKKRISLGLKQLAPDPWDELE
                     TNYPPGSIVKGKVKNFTEFGMFVGIGNGIDGLVHMSELSWSRRKKAFQEQLKKGTTID
                     AFVLNIDKAQKKFSLSLKRLKEDPWKGVGNRYSTGDVVEGHITSITDFGVFVEIEEGI
                     EGLIHVSEMDELQGKQPSEVFTLDDTIQAAILNIDEKDKRIALSTRVLRRKENRVNTE
                     TREESTGAFSTLGDILEPAMKKNSGDSTD"
BASE COUNT          364 a          575 c          584 g          537 t
ORIGIN      
        1 ggttgaggag cttggaggtt atgcccatga tctcccagtt gccgaagtgg aaggtgagcg
       61 cgatgacgcc gcggcctgcg ccaagggttc tttccacgat gtccttgggt tccagggaga
      121 acctttccgg gacctcgcgg ggagggagaa aggggacgag aagaagctct atcacgttgg
      181 tgccgagctt ctggaaacag gagagggcga tgtctccggc cttgttgggc gacagatcgg
      241 ggaaggccct gcgtatgttg gagatggcaa tgttcctgcg tcttcgcagg aggtggaatc
      301 ccatcctgcc gagcagcctg ccggtgccct ggcgggcgct ctcgggaagg gccatgaggg
      361 cgcactgcag tgccttgata agagcgatga ttacgatatc cgcgatcaca ccggcctatt
      421 tcggatcgtc gggtttgatc ggcagggctt cctcgatgag catgacaggt atgccgtccc
      481 ggatgggata aagaagacgg caggccgcgc acgtgagacc gttcccggac gccgtcagga
      541 caaggtcgcc tttgcatttg ggacaacaga gtatgtcgag tagatcctgg ctgatggtca
      601 ttttcttcct tccttagggt caatccgtgc tgtcgccact gttcttcttc atggccggct
      661 caagaatgtc gcccagggtt gagaaagcgc ccgtgctctc ttcgcgcgtc tccgtgttca
      721 cccggttctc tttcctgcga agtaccctgg tgctgagtgc tatcctcttg tccttttcgt
      781 cgatattgag gatggccgcc tgtatcgtgt catcgagggt gaagacctcc gagggctgtt
      841 tcccctggag ctcgtccatt tcggagacgt ggatgaggcc ctcgatcccc tcctctatct
      901 cgacgaatac accgaaatca gtgatgctcg ttatgtggcc ctccaccacg tcacccgttg
      961 aataacggtt tcccacgccc ttccaggggt cttctttcag gcgtttgaga cttaaggaga
     1021 acttcttctg tgccttgtct atgttgagaa cgaacgcgtc tatggtggtg cccttcttga
     1081 gctgttcctg gaatgccttt tttctcctgg accaggaaag ttccgacatg tggacaagtc
     1141 cgtcgatgcc gttgcctatg ccgacgaaca taccgaactc ggtgaagttc ttgaccttgc
     1201 ccttcacgat ggagccaggc ggatagttcg tctcaagctc atcccagggg tcgggagcga
     1261 gctgtttaag gcccagggag atgcgtttct tctcgaggtc gatcttgagg acgacgagtt
     1321 ccaggttgtc acccttgttg acgagctttg cagggttctt gaacttcttg tcccagctca
     1381 tctcgctgac gtggagcagt ccctcgagcc cctgctcgag ttccacgaat gccccgtact
     1441 ccacgatccc cacgaccttg cccttcacct tgcttccgac ggggtacttc tcgtctatct
     1501 tcagccacgg gtcggtcttg agctgtttca tgcctatgga gacacgtccc ttctcggtgt
     1561 cgatggctat gatcttgacc ttcacctcgt ccccgatcct gaggtattcc ttggggtggg
     1621 tgatcttgcc ccatgttatg tcgtttatgt gcagaaaacc atcgacaccg ccgaggtcga
     1681 caaaggcccc gtagtcggtg atgttccgga cgaaaccata catgatctgt ccgtccttga
     1741 cggtcttcca gaattcctgt ctcttcttgt ctctttcgtc ttcgaggagt acccgtctcg
     1801 agaggatgat gctgcccttt ctccggttgg acttgatgac cttgaacttc aggttccttc
     1861 ccacgaatga tgaagggttc ttgacgggtt tgatatccac ctgcgagaga gggaggaagg
     1921 cgttgatccc catatcgacg atgaacccgc ccttgacctc gttcaggata tgaccttcca
     1981 ggggcgtgcc gtcttcgagt gacttgtcga tcttttccca ggtcttgatc tcgtcgaccc
     2041 tcctcttcga gagcctcagg
//
LOCUS       DAOPOA010000465         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58848_length_2059_cov_3.084331, whole genome shotgun sequence.
ACCESSION   DAOPOA010000465 DAOPOA010000000
VERSION     DAOPOA010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58848_length_2059_cov_3.084331"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PKJ17_09275"
     CDS             <1..811
                     /locus_tag="PKJ17_09275"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOD76206.1"
                     /translation="ALGMPILGTQESVEGLSGDELKGYFKGFYGPENTIITISGRVNH
                     ETCVEEIGRRFFGIPAGRADGRELVPPRATTGIDIYEKDLEHIYLCIGTDGVSQVDRR
                     RYVLYVLNAIMGGSMSSHLFQEIREQRGLVYNIYSYVNCYHDAGTFGISTSTSQESMM
                     EVLTLIREEIARIRDRGITDAELSFSKEHIKGNLFIALEGSETRMGRLAKNEIYFGTY
                     IPLKETLREIDRITKKDVAAIAREIFDSTKVVSLTVLGNVDREKVEKVWRE"
     gene            799..1239
                     /gene="dut"
                     /locus_tag="PKJ17_09280"
     CDS             799..1239
                     /gene="dut"
                     /locus_tag="PKJ17_09280"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOD76207.1"
                     /translation="MERIEVFIKMRDGATVPAYATDRASGVDLCALVDGPVVLGPMER
                     ALIPTGIFIDIPEGFEAEVRPRSGIAHKHGVTVLNTPGTIDSDYRGEVKVILINLGGD
                     PFTIRSGDRIAQMVFKRLARVDFRPVASLADSCRGEGGFGSTGV"
     gene            1254..>2059
                     /locus_tag="PKJ17_09285"
     CDS             1254..>2059
                     /locus_tag="PKJ17_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOD76208.1"
                     /translation="MERLYHHLDSYVRHLVSERGASPHTVEAYNRDILTFIRYCEDIA
                     CPEPERRHVEAFIGYMRENGRSTRSIVRSLSALRGFFNYLLLDGRIKVSPLDDVDTPR
                     FRPPIPRVLSEDEMGELIHLPEGGRMALRDRTMLELLYATGLRVSELIRLKKGDINLD
                     GGFVVAMGKRSKERVVPLGSYSRDAVKAYLDEERPQGQYLFPNRRGGMITRQAVWKII
                     RKYGLKLSRGKVSPHTIRHTFATHLLEGGADLRSVQVLLGHEDISTTQIYT"
BASE COUNT          461 a          565 c          651 g          382 t
ORIGIN      
        1 tgccctggga atgcccatac tgggtacaca ggagtcggtg gaggggctta gcggcgatga
       61 gctgaagggt tatttcaagg ggttctacgg gccggagaac acgataatca ccatcagcgg
      121 cagagtgaac cacgagacct gcgtggagga gataggacgg cgttttttcg gcatcccggc
      181 aggaagggcc gacggcaggg agctcgtccc gccgcgcgcg acgacgggga tagacatata
      241 cgaaaaggac ctggagcaca tctacctgtg cataggcacg gacggtgtca gtcaggtcga
      301 caggcgcagg tacgtgctct acgtgctgaa cgcgataatg ggcggcagca tgagctccca
      361 ccttttccag gagatacggg agcagagggg cctcgtgtac aatatctact cctacgtcaa
      421 ttgctaccat gacgcgggta cgttcgggat atccacgtcc acgtcgcagg aatccatgat
      481 ggaggtgctc actctcatca gggaggagat agcgcgcatc cgggacaggg gcataaccga
      541 tgccgagctt tccttttcga aggagcacat aaagggcaac ctcttcatcg cccttgaggg
      601 ctcggagaca cggatgggcc gcctggcgaa gaacgagata tacttcggga catacatacc
      661 cctcaaagag accctccggg agatagacag gatcaccaag aaggacgtgg ctgccattgc
      721 ccgcgagata ttcgacagca cgaaggtggt gtcgctgacc gtgctcggca acgtggaccg
      781 cgagaaggtc gaaaaggtat ggagagaata gaggtcttca tcaaaatgcg cgacggtgcg
      841 acggtgccgg cgtacgctac ggaccgtgcc tccggggtcg atctgtgcgc cctggtcgat
      901 ggtcccgttg tccttggacc catggaacgg gcccttatcc cgacgggcat cttcatagac
      961 atacccgaag gtttcgaagc ggaggtgcgt ccccgcagcg gcatcgccca caagcacggg
     1021 gtgacggtgc tcaacacacc gggcaccatc gattccgatt accggggcga ggtcaaggtg
     1081 atcctcatca acctgggcgg agaccccttc acgataagaa gcggagatag gatcgcccag
     1141 atggtcttca aaaggctggc cagagtggat ttccggcctg tcgcgagtct cgccgattcg
     1201 tgccgcggcg agggaggttt tggttccacg ggagtatagg ggagatacgg caggtggaac
     1261 gtctctatca ccatctcgac agttacgtcc gtcacctcgt ctcagaacgc ggggcttcgc
     1321 cgcacacggt cgaggcctac aaccgggata tcctgacatt catacgatat tgtgaggaca
     1381 tagcctgccc ggagccggaa aggcgccatg tggaggcctt tatcgggtac atgcgcgaaa
     1441 atggcaggtc gacgcggagc atcgtgagga gtctttccgc cttgagaggt ttcttcaatt
     1501 atcttcttct cgacggcagg atcaaggtca gtcctctcga cgatgtggat acgccaagat
     1561 tccggccgcc cattccccgg gtgctttccg aggacgagat gggtgaactc atccacctgc
     1621 ccgagggggg gaggatggcc ctgagagacc ggacgatgct cgagctcctc tacgcgacgg
     1681 gtctgagggt ctcggagctc atacggctca agaagggaga tatcaacctg gatggcggct
     1741 tcgtcgtcgc catgggaaag cgttccaagg aacgggtcgt gccgcttggc tcctactcga
     1801 gggatgccgt caaggcctac ctggatgagg aaaggcccca gggccagtat ctcttcccca
     1861 accgccgcgg aggcatgata acccgccagg ctgtctggaa gatcatacgg aaatacggtc
     1921 tcaagctgtc aagaggcaag gtatcgcctc atacgatacg gcataccttt gccacgcacc
     1981 tcctcgaggg cggcgcggac ttgaggtcgg tccaggtgct cctgggacac gaggacatat
     2041 cgacaacaca gatatacac
//
LOCUS       DAOPOA010000466         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_58995_length_2055_cov_3.838500, whole genome shotgun sequence.
ACCESSION   DAOPOA010000466 DAOPOA010000000
VERSION     DAOPOA010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58995_length_2055_cov_3.838500"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PKJ17_09290"
     CDS             complement(<1..1075)
                     /locus_tag="PKJ17_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOD76209.1"
                     /translation="MNNRVRNVGIMAHIDAGKTTATERILFYTGVNNRIGEVDDGAAT
                     MDWMEEEKERGITITAAATTCFWRDHRVNIIDTPGHIDFTIEVGRSLRVLDGAVALFS
                     GVEGVEPQSETVWRQADRYGVPRIGFINKMDRPGADFDKCTAMMRDILRANPLALQIP
                     LKNGDEFIGVIDIIEEKMMRYDKDRLGLDCADEEVPAEYRDRVHYMREAMLESLAEID
                     DHIMTRYLEGGDIGTDAIRGAIRKGTLEGRILPVLCGSAFKNKGIQPLLDAIVDYLPS
                     PDDVSPYQCWTSDGEELSLRGTSEEPMSALVFKIMTDPFVGHLSYIRVYSGEIKAGDT
                     IINTSRDRSERVGRILRLHANKRE"
     gene            complement(1086..1556)
                     /gene="rpsG"
                     /locus_tag="PKJ17_09295"
     CDS             complement(1086..1556)
                     /gene="rpsG"
                     /locus_tag="PKJ17_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOD76210.1"
                     /translation="MPRKGHISKREKTPDPKYNDMMVQRLINCVMLDGKKSTASGIVY
                     GAINLIEERLKEEGLKVFHKALDNIKPEIEVKARRVGGATYQVPVEVRLNRKLSLGIR
                     WLIRYSRARSEKTMMERLAGELIDAYNNKGSAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(1570..1944)
                     /gene="rpsL"
                     /locus_tag="PKJ17_09300"
     CDS             complement(1570..1944)
                     /gene="rpsL"
                     /locus_tag="PKJ17_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOD76211.1"
                     /translation="MPTINQLVRLGREAVKKKSSSPALTNCPQKRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNSIEVTTYIPGIGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGSL
                     DASGVANRKKSRSKYGAKKPKQ"
BASE COUNT          360 a          603 c          545 g          547 t
ORIGIN      
        1 cttcgcgctt gttcgcgtgg agcctcagta tcctccccac gcgctccgat ctgtcgcggg
       61 acgtatttat aatggtatcg cccgccttga tctcaccgga gtagacccgg atgtagctca
      121 ggtggccgac gaagggatcg gtcatgatct tgaagacgag cgcgctcatg ggctcttccg
      181 atgtccccct gaggctcagt tcctcgccat cgcttgtcca gcactggtaa ggcgatacgt
      241 cgtccgggga cggcaggtag tcgacgatgg cgtcgaggag cggctggata cccttgttct
      301 tgaaggcgct gccgcacagg acgggaagta tcctgccttc gagagtcccc ttcctgatgg
      361 cgcccctgat cgcgtcggta ccgatatcgc cgccttcaag gtaacgggtc attatgtggt
      421 cgtcgatctc cgcgaggctt tcaagcattg cctccctcat gtaatggacc ctgtccctgt
      481 actccgcggg gacctcttca tcggcacagt caaggcccag acggtccttg tcgtacctca
      541 tcatcttctc ttcgatgata tcgatgacgc ctatgaactc gtcaccgttc ttgagaggta
      601 tctgaagcgc gagggggttt gccctgagga tgtcgcgcat catggccgtg cacttgtcga
      661 aatccgctcc cggcctgtcc atcttgttga tgaagccgat acgcggaaca ccgtagcggt
      721 ccgcctgtct ccagacggtc tccgactgcg gttccacacc ttcgacgccg gagaaaaggg
      781 caaccgctcc gtcgagtacg cgaagggacc tgccgacctc gatggtgaaa tcgatatgcc
      841 ccggggtgtc tatgatattg acgcgatggt cacgccagaa acaggtggtc gcggcggcgg
      901 tgatggtaat gccccgctcc ttctcttcct ccatccagtc catcgttgcc gctccgtcgt
      961 caacctcacc tatcctgttg ttgacgccgg tatagaaaag gatccgctcc gtcgcggtcg
     1021 ttttgcccgc gtcgatgtgc gccatgatcc ccacgtttct gacccgattg ttcattgtaa
     1081 acctgttacc acctgtagtg ggcgaatgcc ttgttggcct cggccatctt gtgggtgtcc
     1141 tctctcttct tgaccgcgct ccccttgttg ttgtaagcgt cgatcagctc gcccgcaagc
     1201 ctctccatca tcgtcttctc tgaacgcgcg cgggaatagc ggataagcca tcgtatgccg
     1261 agggagagtt ttctgttgag cctcacttct acaggtacct ggtaggtggc gccgccgaca
     1321 cgtctcgcct tgacctcgat ctcgggcttg atgttgtcga gggccttgtg gaaaaccttg
     1381 agaccttcct ctttgagcct ctcttcgatg aggtttatag caccgtacac gatcccggac
     1441 gccgtgctct tcttaccgtc gagcataacg cagttgataa gcctctgaac catcatgtcg
     1501 ttgtacttgg gatcgggtgt cttctcccgt ttggatatgt gtcctttgcg tggcatttct
     1561 tatgctccct tattgtttcg gtttctttgc cccatacttg gacctgctct tcttcctgtt
     1621 cgcgactccg ctggcgtcga gggatcccct gatgatgtgg tagcggacac cgggcaagtc
     1681 tttcaccctg ccgcccctga tgagaaccac ggagtgttcc tgcaggttgt ggccgatgcc
     1741 ggggatgtac gtcgtcactt cgatgctgtt cgtaagcctt acacgggcga ccttcctcaa
     1801 tgccgagttc ggtttcttcg gtgtcgtcgt atagaccctg acgcaaactc cccttttttg
     1861 cgggcagttt gtcagagcgg gagacgagct cttctttttg acggcttctc tcccaagcct
     1921 cactaactgg ttaatagtag gcatcaatac ctccaaaaaa ataatccctc atcatgacgg
     1981 gatataggtc ataactggcg taaaaagcag tgctatatta aacgattacc ttttttattg
     2041 tcaagacttt tttgg
//
LOCUS       DAOPOA010000467         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_59074_length_2053_cov_2.284785, whole genome shotgun sequence.
ACCESSION   DAOPOA010000467 DAOPOA010000000
VERSION     DAOPOA010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59074_length_2053_cov_2.284785"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1150)
                     /locus_tag="PKJ17_09305"
     CDS             complement(152..1150)
                     /locus_tag="PKJ17_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HOD76212.1"
                     /translation="MRTICPYCEDVKDVEVVDTTENIKVKGELIEVPVTYYKCSACSG
                     TFDDPKTGGDPLERAYMEYRSRHGMIQPAEFKEFRKRHGLTQAEFNTLLGWGKVTVNR
                     YESGALQDEAHDKEARLAMDPRNLINLIEETPSALSDQKRARLLESLRREEEETFSFE
                     RIFEERFGKYEASELSGYRRLNVPRVLKAILFFCKGGDFKTKVNKLLFYADFSHFKEN
                     TTSITGAHYAHATHGPVPDNYELYFAHLVENGSLKVEEVPCGSDHIAERLITVNMPDL
                     FSLFSESDVKTLISVKEFFRDYTATAIRDFSHREKGYQETTDGQPISYEYADALQI"
     gene            complement(1147..1524)
                     /locus_tag="PKJ17_09310"
     CDS             complement(1147..1524)
                     /locus_tag="PKJ17_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76213.1"
                     /translation="MPEEKREAVRAFLRAFKKVAHERGIYFIPRKGFTETLAWLGYTK
                     TNCRDEILGLTVDDYCKGPEPDRDMPGEVWVFGMNMSGSNIYIKLKLTKAGSQTIAKC
                     VSFHPAKHNLRFPLRDEGEGEKK"
     gene            complement(1606..1758)
                     /locus_tag="PKJ17_09315"
     CDS             complement(1606..1758)
                     /locus_tag="PKJ17_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76214.1"
                     /translation="MKRPFRVTWKTPTGETATVDIEAESKGQARRIFIQRGGELGQIV
                     TMEELP"
     gene            complement(1780..>2053)
                     /locus_tag="PKJ17_09320"
     CDS             complement(1780..>2053)
                     /locus_tag="PKJ17_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76215.1"
                     /translation="WTYQHLMAINGKFSDISRGDLLAVADRFSIGRAEEIIEHVDDAV
                     ARWPQYAREAGIAPEEIKRIGEDHRLARSSLMEPAVWTGSDLTMRD"
BASE COUNT          388 a          583 c          533 g          549 t
ORIGIN      
        1 acgatttttt catcaggggc agggcgagcc cgatcggcac gccattgaca tacttccacg
       61 gttgtcatga acgccagaag ctgtttcgct ccttaggctc ctctattgcc acgatctgca
      121 gtatctcccc gccgcgcgca caagaggctc cctatatctg cagggcgtca gcatattcat
      181 atgagatagg ctggccgtcc gtggtttctt gatatccttt ttccctgtgg gagaagtccc
      241 ttattgctgt cgcagtgtag tcccggaaga attccttgac ggaaatcagc gtctttacgt
      301 cgctttcgga aaagagtgaa aagagatcgg gcatgttgac agttatgagt ctctcggcga
      361 tatggtcgga tccacatggc acttcttcga cctttagtga gccgttttca acgaggtgcg
      421 cgaagtagag ttcatagttg tcgggaacgg gaccatgggt ggcatgcgcg taatgggctc
      481 ccgttatcga tgtggtgttt tccttgaagt ggctgaagtc ggcatagaag aggagcttgt
      541 ttaccttggt cttgaagtca ccgcccttgc agaagaagag tatcgctttc aggaccctcg
      601 gcacgttgag acgcctgtaa ccgctcagct cgcttgcctc gtatttcccg aagcgttcct
      661 cgaagatcct ctcgaaggag aaggtctctt cttcctccct tctgaggctc tcgaggagcc
      721 gcgccctctt ctggtcgctg agcgcggacg gtgtttcctc tatcaggttg atcaggttcc
      781 gggggtccat cgccaggcga gcttccttgt cgtgggcctc gtcctgcagt gccccgctct
      841 cataccgatt gacggtcacc ttgccccagc cgagcagggt gttgaactcg gcttgcgtca
      901 ggccgtgcct ctttcgaaac tccttgaact ccgcgggttg gatcatccca tgccgggagc
      961 ggtactccat ataggccctt tccaggggat ccccgccggt cttcgggtcg tcgaaagttc
     1021 cactgcaggc tgagcattta taataggtga caggaacctc tataagctcg cccttgacct
     1081 tgatgttctc agtggtgtcg accacctcca cgtccttaac gtcttcgcag tacgggcaaa
     1141 tggttctcat ttcttttctc cttccccttc atctctgaga gggaaacgaa ggttgtgctt
     1201 tgccggatgg aaggacacgc atttcgcgat ggtttgtgac ccggcctttg tcagtttcag
     1261 tttgatatat atgtttgagc cactcatatt cataccgaag acccagactt caccgggcat
     1321 gtcccggtca ggctccggtc ctttacagta atcgtcgacg gtgagaccca ggatctcatc
     1381 gcggcaattg gttttcgtat atcccagcca tgccagggtt tcggtgaaac ccttacgcgg
     1441 gataaaatag atgccacgtt catgtgcgac cttcttgaat gctctcagga acgccctgac
     1501 cgcctcccgc ttttcctcag gcactattca ctccacgatt cctcttgtag attaattata
     1561 tcacgttatg ataagttggc aagataaaag ttatcatatg atattttaag gaagctcctc
     1621 catcgttacg atctgcccca gttccccgcc gcgctggatg aagatccgtc tggcttggcc
     1681 tttgctctcg gcctctatgt cgacggttgc cgtctcgccg gtcggggtct tccaggtgac
     1741 gcggaaagga cgcttcatac tcatactccc gtcatgtgtt caatctctca ttgtgagatc
     1801 cgagccggtc cacacagccg gctccattag agaggacctt gccaggcgat ggtcctcccc
     1861 gattcgtttg atctcctccg gcgcgattcc cgcttcccgg gcatattggg gccaccgggc
     1921 aacggcatcg tcaacatgct cgattatctc ctccgccctt cctatgctga acctgtccgc
     1981 aactgccaac aggtcgccgc gggagatgtc ggagaacttc ccgttgatgg ccatgagatg
     2041 ctggtaggtc cat
//
LOCUS       DAOPOA010000468         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_59199_length_2049_cov_4.219659, whole genome shotgun sequence.
ACCESSION   DAOPOA010000468 DAOPOA010000000
VERSION     DAOPOA010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59199_length_2049_cov_4.219659"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PKJ17_09325"
     CDS             complement(<1..940)
                     /locus_tag="PKJ17_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOD76216.1"
                     /translation="MTEQAFYEIALILSLAAVLGAVGMLLRQPLIVAFLATGILAGPA
                     GLSLIQSHGQIELLAQIGISLLLFVVGLRLDLTLIRTTGPVALATGLGQVIFTSLFGF
                     LITLWLGFSPVASLYVAVALTFSSTIIIVKLLSDKKEIDALHGRIAVGFLIVQDIVAI
                     LAMIILTAVGSGGASDGATFSRIAFTALKGIGFLICIGLLMKYVLPGLTKRLAWNQEL
                     LLLFSITWAVFLGAAGDYLGFSKEVGAFLGGVSLASTQYREAMAARLVSVRDFLLLFF
                     FIDLGARLDFNTVGPQIPNAIYLSLFVLIGNPLVVMA"
     gene            complement(1027..>2049)
                     /locus_tag="PKJ17_09330"
     CDS             complement(1027..>2049)
                     /locus_tag="PKJ17_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOD76217.1"
                     /translation="AMITGDHAVTASAIGRMIGLGESGSPAITGKELERMSDDDLFGK
                     VQETSVYARVSPQHKLRIVQQYMRHGEIVAVTGDGVNDAPALKAAHIGAAMGKTGTDV
                     AREAADMVVTDDNFASIFHAVEEGRIVFDNIRKVTVFLIPTGFAAILSILACMFLGIP
                     IPYVAAQLLWINLVANGLQDVALAFEPGEKDIVKRQPRSPKEGIMSRLMLERSILVGV
                     LISAGVIYNFHDALTQGSSVEHARTIAMTTMVIFQFFHAWNSRSETRSVFRLNPLSNP
                     FLFYSMIVASLAQIAVIYVPTLQWVFRTDNLSGGEWLRIIAVAVSAIAAVEIEKLLRG
                     NRRPKG"
BASE COUNT          445 a          582 c          644 g          378 t
ORIGIN      
        1 tggccatgac gacgagggga ttcccgatga gaacgaagag agacagatag atcgcattgg
       61 gtatctgggg ccctacggtg ttgaaatcaa ggcgtgcgcc gaggtcgatg aagaagaaaa
      121 ggagcagaaa atccctgacg ctgaccaaac gggccgccat ggcctcccgg tattgcgtcg
      181 aggcgaggga aacgccgccg aggaaggcgc cgacctcctt gctgaaacca agatagtctc
      241 ccgcggctcc caggaacacc gcccaggtga tggaaaagag aaggagcaat tcctggttcc
      301 aggcgagccg tttcgtcagg ccgggaagga catatttcat gagcagtccg atgcagatga
      361 ggaaaccgat ccctttgagc gccgtaaagg cgatgcgcga gaacgtcgcg ccgtccgagg
      421 cgcctccgct ccccacagcc gtcaggatga tcattgccag gatggccact atgtcctgca
      481 cgatgaggaa cccgacggca atcctcccgt gaagggcgtc gatctctttc ttgtcggaaa
      541 ggagcttgac gatgatgatg gtgctggaga aggtaagggc aacggccaca tagagcgagg
      601 ctacagggga aaaaccgagc cagagagtta tgaggaaacc gaagagactg gtgaagatca
      661 cctggccgag accggtcgcg agcgccaccg ggcctgtcgt ccggataagg gtgagatcga
      721 gcctgagtcc gacgacaaag agaaggagcg atatcccgat ctgtgccagg agctctatct
      781 gcccatggct ctggataagg gaaaggccgg cgggcccggc gaggatgccc gtcgccagga
      841 aggcgacgat aaggggttgc ctcagcaaca ttcccaccgc gccgaggacc gcggcaaggc
      901 tgaggatcag cgcaatctca tagaaggcct gttccgtcac gttcttttcc cgtccgtatt
      961 attgcagtat gaagggaccg aggtcaagtg ctctccgtcc gccgggtgaa gagcgcgatc
     1021 caccttttag cctttcggtc tgcggttccc gcgcaggagc ttttcgatct cgaccgccgc
     1081 gatcgcgctg accgccacgg cgattatccg cagccattcc cctcccgaga gattatccgt
     1141 cctgaaaacc cactgaagcg tgggcacata gatgacggcg atctgagcca gagatgccac
     1201 gatcatactg tagaagagga aggggttgct taaggggtta agacggaaga cggaccgggt
     1261 ttcagaccgt gagttccagg cgtggaagaa ctggaagatg accatagtgg tcatggcgat
     1321 cgtccgggcg tgctccacgg aggaaccctg ggtcagggcg tcatggaagt tgtaaatgac
     1381 acccgccgag atcaggacgc cgacgaggat gctccgctcg agcatcagac gcgacatgat
     1441 accttctttc gggctccgcg gctgcctctt gacgatgtcc ttttcccccg gctcgaaggc
     1501 gagggccacg tcctgcaggc cattggcgac taggttgatc caaaggagct gggccgccac
     1561 gtaggggatg ggtatcccga gaaacatgca ggcgaggatc gaaaggatgg ccgcgaaccc
     1621 cgtgggtata aggaacactg tcaccttccg tatattgtcg aaaactatcc ttccctcctc
     1681 cacggcgtgg aagatggacg cgaaattgtc gtcggtcaca accatgtcgg cggcctcgcg
     1741 ggcgacgtcc gtccccgttt ttcccatggc cgctcctatg tgcgccgcct tcagggcggg
     1801 ggcgtcattg acgccgtctc cggtgacggc cactatctct ccgtgccgca tgtactgctg
     1861 aacgatgcgc agcttgtgct gcggcgagac gcgggcatag acgctggtct cctggacttt
     1921 gccgaagagg tcgtcatcac tcatcctttc cagttccttc cccgtgatgg cgggggatcc
     1981 gctttccccc aggcctatca tcctgccgat ggccgaggcg gtcacagcgt gatcgcccgt
     2041 gatcattgc
//
LOCUS       DAOPOA010000469         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_59938_length_2031_cov_3.654352, whole genome shotgun sequence.
ACCESSION   DAOPOA010000469 DAOPOA010000000
VERSION     DAOPOA010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59938_length_2031_cov_3.654352"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PKJ17_09335"
     CDS             complement(<1..810)
                     /locus_tag="PKJ17_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HOD76218.1"
                     /translation="MRYRKVLGTLVFVVCALVLALVRPFTGLSGEGHYVCSTIFMALG
                     LWIFRPGGVPYLAGSAVLIAGSVLFGVPLGVVTSGFTSSGVWVLIPALYLGYALAKTG
                     LGKRIAYFVLKIFRPGYFTIVLSWFIIGLVLSALTPSITVRLSVVMPIAMNLVEGCGL
                     KDRSRGSALICLAAWGGAVLPGTGWLTGSLWGPFMMGFLPPAMKQFATFDNWFRIMSV
                     PWLIAGVFFVGLVYLLLKPKAPLGVARESFHEAYRALGRISAREIVTAAILT"
     gene            complement(897..>2031)
                     /locus_tag="PKJ17_09340"
     CDS             complement(897..>2031)
                     /locus_tag="PKJ17_09340"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD76219.1"
                     /translation="GETPFFELTVALASGNAGSMTSIPGLSFHRCGSLHIGGTHIEDD
                     IDLIPARDLLPVERYRIGKRPYTFLLTSRGCPFGCSFCGKPPVPYRRRALQSIEKELS
                     GIERLGITSIDFEDDMLNLDIPFFHRVLDLVKGRGFVLSAMNGLYSHTLDTATLTKMR
                     DAGFSRLNFSLVDTSPTVHSTQRRRYPANFLGLLDWLESSDFLVETHFIIGLPGQTPR
                     EIIDTMTFLAGKRMLPGPSVYYPAPGSPLFDDIRGGEASHFFPMTRSSVMFQANPLFP
                     RETTFTFMKLLRFMNVVKALIDANPGASALVDLAHADRLKKNPRDLHIFTSLLRERRF
                     LWFDTRGNDYVEEPQDHALVAAFFGALEGMTIKGFRTSNTVRA"
BASE COUNT          465 a          577 c          650 g          339 t
ORIGIN      
        1 cgtgaggata gcggcggtaa cgatctcccg tgcgctgatc ctgccgaggg cacggtatgc
       61 ctcgtggaaa gattccctcg ctacccccag gggggccttc ggcttcagaa ggagatacac
      121 gagaccgacg aagaaaacgc cggcgatgag ccatggcacg gacatgatcc ggaaccagtt
      181 gtcaaaggtg gcgaactgct tcatggccgg gggaaggaat cccatcatga aaggccccca
      241 caaggacccc gtcaaccacc ccgtacccgg cagaacggcc cctccccagg cggcaagaca
      301 gatgagggct gagccccggg acctgtcctt caacccgcac ccctcgacga ggttcatggc
      361 gatgggcata acgacggaaa gacgcaccgt tatcgacggt gtaagcgcgg agagaacgag
      421 cccgatgatg aaccaggaca ggacgatggt gaaataaccc ggtcgaaaga tcttcagcac
      481 aaagtaggcg atccgcttgc cgaggcctgt tttcgccagg gcgtagccga gatagagggc
      541 cgggatgagc acccagacgc ccgaacttgt gaaaccgctc gtcacgaccc cgaggggcac
      601 gccgaagagc accgaaccgg cgatgaggac ggccgatcct gcaaggtaag gcacacctcc
      661 cggtctgaag atccacaggc cgagggccat gaaaatcgtc gaacagacgt aatgtccctc
      721 tcccgacaac ccggtgaaag gtcgaacaag ggccagaacc agcgcgcaaa cgacgaagac
      781 cagtgtaccc agaaccttcc gataccgcac aggcgttcct ttcgacagga tgtaaggggt
      841 taggatacca cattttcgat cgccgtgcag gccttttgtc ggccccggcc gccccgtcag
      901 gccctcacgg tgttcgatgt gcgaaatccc ttgatggtca ttccctccag ggctccaaag
      961 aaggcggcca cgagggcatg gtcctgcggc tcttcgacat agtcgttgcc ccgggtgtcg
     1021 aaccagagaa agcgcctctc ccttagaagg gaggtgaata tgtgaaggtc ccgggggttc
     1081 ttcttcagcc ggtctgcgtg ggccaggtcg acaagggcgg aagctccggg gttcgcgtcg
     1141 atgagggcct tgacgacgtt catgaacctc aggagcttca tgaaggtgaa cgtcgtctcc
     1201 cgcgggaaga ggggattcgc ctgaaacatg acgctcgagc gcgtcatcgg gaagaaatgt
     1261 gaggcctctc ccccgcggat gtcgtcaaac agcgggctgc ccggggcggg gtaatagacg
     1321 ctcgggccgg gcagcatcct ctttcccgcg agaaaggtca tcgtgtctat gatctctcgc
     1381 ggtgtctggc ccggaaggcc gatgatgaaa tgggtttcca cgagaaagtc cgaagactcc
     1441 agccagtcga gaaggccgag gaagttcgcc ggatacctcc ttcgctgcgt gctgtggact
     1501 gtcggcgagg tatcgacgag ggagaagttg agacgcgaga aaccggcgtc gcgcatcttc
     1561 gtcagtgtgg ccgtatcgag ggtgtggctg tagagaccgt tcatggcgga aaggacgaac
     1621 cctctcccct tcacgaggtc aaggacacgg tggaagaagg ggatgtcgag gttgagcatg
     1681 tcgtcctcga aatctatgga ggttatcccc agcctctcaa tgccggaaag ttccttctcg
     1741 atggactgaa gggcccggcg gcggtaggga acgggaggct tgccgcagaa ggaacacccg
     1801 aaggggcacc ctctcgacgt gaggagaaag gtgtacggcc tcttgccaat gcgatacctc
     1861 tcgacgggaa gaagatcgcg cgccggaatg aggtcgatgt catcctcgat atgagtgccc
     1921 ccgatatgaa gggacccgca gcggtgaaag gagagtcccg ggatggaggt catactgccg
     1981 gcattccccg aagcaagggc gacggtcaat tcgaaaaaag gcgtctcccc t
//
LOCUS       DAOPOA010000470         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_60067_length_2028_cov_3.129245, whole genome shotgun sequence.
ACCESSION   DAOPOA010000470 DAOPOA010000000
VERSION     DAOPOA010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60067_length_2028_cov_3.129245"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(486..1775)
                     /locus_tag="PKJ17_09345"
     CDS             complement(486..1775)
                     /locus_tag="PKJ17_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOD76220.1"
                     /translation="MDDPLTGVIIIIVLLILNGVFSAAETAIVASRKSKIKELLRKKK
                     DRKTEMLLSLKENPERFLSTVQIGITLFGTLASAVGGLMAVKYLMPVIGKVEFLKPFS
                     ESIAVGVIVCILTYLFIVFGELVPKYVGLTYREKAALIVLPFFNLVSRVFSVFVNFLS
                     VTTLFIVKALNLSRSEEHIGEGEIKILLEEGRRKGLFDKTEEELINRIFHFSDRSVRE
                     VMVPRPNVYAIDVDDSRDNIMNYIIGNEFSRYPVYRENFDNVIGFVYQKDITRHIWKT
                     QEPFELEKIVKRPFFVPATMEISVLLKQMQRTRRHLAVVIDEYGSAVGIITLEDIMEE
                     IFGEIMDETDVDDKIERQRDGSYLIDASYSVRDLNNRLNLDLPESPDYETLGGFITTQ
                     LQGLAKGGEIIHHGRHRFTVVDIDGRRIVKVKFERVK"
     gene            complement(1775..>2028)
                     /locus_tag="PKJ17_09350"
     CDS             complement(1775..>2028)
                     /locus_tag="PKJ17_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76221.1"
                     /translation="SFEGEAVLTVGKSVDFARKGVAGIINAMPFTCMPGTVSSAIMRL
                     IQQNHDIPVINIAYDGQGVTNILTRLEAFMHQVRERTRD"
BASE COUNT          444 a          560 c          563 g          461 t
ORIGIN      
        1 ctagaataac cctcccgtta cggatgagac ctctcaatct ctccgggacg ttctcaacgg
       61 gcgtcaccag cctgaagaaa aaactcctgc tgttgcctat gcgaaacaac cctgccacaa
      121 tgatcaataa gagcgacaag tcaagcaaca acaggaaatt caggtagaac gcagacagac
      181 tcatcggacg ccctccgcgg catctctcgt ctcacaggac tcgaaatggc atgcatcgaa
      241 tccaatcaac ttcatcctcc cttttttgat ctccaccaga tcagaacccg aaattccgca
      301 gtccgcagaa catatgcttc agaggaaggg agaagacaaa ccgccaggat cgccgcccct
      361 ggtagagaga gttcaggaga taactcattg aatcaacttt tcgcttattc tattgatgga
      421 ccagggaaaa atcccatgag aagttacagg tctgccgtgt taagggacgg gagtcgtctt
      481 ctccactatt tcaccctttc gaacttgacc tttactatcc ttcggccgtc tatgtcgacg
      541 acggtgaagc ggtggcggcc gtggtggatg atctcgccgc ctttggcaag gccctggagc
      601 tgggtggtta tgaagccgcc caaggtctcg tagtcggggg attcggggag gtccaggttc
      661 aggcggttgt tgaggtcgcg gacggaatat gacgcgtcga tgaggtagga accgtccctc
      721 tggcgttcga tcttgtcatc cacgtcggtc tcgtccatga tctcgccgaa gatctcctcc
      781 atgatgtctt cgagggtgat gatgccgacg gccgagccgt actcgtcgat gaccaccgcc
      841 aggtggcggc gcgtgcgctg catctgcttg aggaggacgc ttatctccat ggtagccggg
      901 acgaagaagg ggcgctttac gatcttctcc agttcgaatg gctcctgcgt cttccagatg
      961 tgccgtgtta tgtccttctg gtagacaaaa ccgatgacgt tgtcgaagtt ttcccggtat
     1021 acgggataac gggagaactc gttgcctatg atgtagttca ttatgttatc cctgctgtcg
     1081 tccacatcga tggcgtacac gttggggcgc ggcaccatga cctccctgac ggaacggtcg
     1141 ctgaaatgga agatcctgtt gatcaactct tcctcggtct tgtcaaaaag accctttcgc
     1201 ctcccctctt ccagaaggat cttgatctca ccctcgccga tatgctcctc ggacctgctc
     1261 aggttcaggg ccttgacaat gaacagtgtc gtaacggaga ggaagttgac gaagaccgaa
     1321 aagacgcgcg acaccaggtt gaagaagggc aggacgatga gggccgcctt ttctctgtat
     1381 gtgaggccga catacttcgg gaccagctct ccgaacacga tgaagagata cgtcaggatg
     1441 cagacgatga ccccgaccgc gatggattcg gaaaagggct tcaggaattc aaccttgcca
     1501 atgacgggca tgaggtactt cacggccatg aggccgccaa ccgccgaggc cagagtgccg
     1561 aagagggtga tgccgatctg gaccgtcgaa aggaaacgct cggggttctc cttcaaactg
     1621 agaagcatct ccgtcttgcg gtcctttttc ttcctgagca gttcctttat cttgctcttg
     1681 cgcgaggcga cgatggccgt ctcggcggcg gagaagacgc cgttcaggat aagcagcacg
     1741 atgatgatta ttactccggt cagcggatcg tccattaatc ccttgtgcgc tccctcacct
     1801 ggtgcatgaa cgcctccagt ctcgtcagga tgttcgtcac tccctggccg tcgtaggcga
     1861 tgttgatgac cggtatgtca tggttctgct ggatgagccg catgatggcg ctcgacaccg
     1921 tgccgggcat gcaggtgaac ggcatggcgt tgatgatccc cgcgacaccc ttgcgggcga
     1981 aatcgacgct cttgccgacg gtgaggaccg cttctccctc gaagctgt
//
LOCUS       DAOPOA010000471         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_60157_length_2026_cov_4.105530, whole genome shotgun sequence.
ACCESSION   DAOPOA010000471 DAOPOA010000000
VERSION     DAOPOA010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60157_length_2026_cov_4.105530"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PKJ17_09355"
     CDS             <1..235
                     /locus_tag="PKJ17_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76222.1"
                     /translation="KRPVVIASYKGELAMGAKYDDARKIFMEEEEFFRLLGSDRKVLF
                     ITKSKRVKNLEKRFPGRITTHLCQNDRCLLSNY"
     gene            255..1784
                     /gene="dacB"
                     /locus_tag="PKJ17_09360"
     CDS             255..1784
                     /gene="dacB"
                     /locus_tag="PKJ17_09360"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOD76223.1"
                     /translation="MTTGNMTAGERDRGHLRYLSMVLVFLTILYALPLLAPAETTGTL
                     NEDLAAILDDPALEAAHVGVVVDSLATGRRIFEYNAGKRFVPASNMKLFTTAAALLAL
                     SPDFRWETRLITDGTVTSGVLSGDIIVEGSGDPTISGYFHEGDVLHVFRRWAAKLKEQ
                     GVREIGGDLVIDNSAFPDKPYGKGWDVDPVNCFSAPRDAFTFNNNCIQLDITPSGRTG
                     EAARIAMEPATDYVSLARGLTVRKNAGPDVVSLEYATPRTLSITGSIGPGTRMITRYV
                     AVNHPAYFGGFVLRETLEAAGIAVKGDILCARNCPKTVDVRTRREKGKWTTVAVYHSP
                     RLAEIVKVVNKLSNNLYAELLLLATGRAAGAAGTEGSAAAALAALRRAGLNTSGVVMA
                     DGSGLSRHDLVTPDSVARLLRIMAQGPHARSFIDSLPVMSVDGTLGVRLKGSRAADRV
                     RAKTGTMTHIRGLSGYVTTMKGERLVFSIFSNNHVATSDVDTATDRIILRLLDHSSSE
                     R"
     gene            complement(1791..>2026)
                     /locus_tag="PKJ17_09365"
     CDS             complement(1791..>2026)
                     /locus_tag="PKJ17_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD76224.1"
                     /translation="FLSPMFLLSGIFFPLHNLPAFVGKVAWFTPLYHLTNVCRALAAG
                     SPGDALEGALWIIVVVVLLSPFPFRLMRRRIIR"
BASE COUNT          448 a          617 c          617 g          344 t
ORIGIN      
        1 caaacgcccc gtcgtgatcg cgtcgtacaa gggagagctc gccatgggcg cgaagtatga
       61 cgacgcccgg aagatattca tggaggaaga ggaattcttc cgccttctcg gctccgacag
      121 gaaggtcctc ttcatcacga aatccaagag ggtaaagaac ctggagaaaa ggttccccgg
      181 acgtataacg acccatctgt gccagaacga caggtgcctc ctgtcgaact actgatccgc
      241 gaggctgtga cacgatgaca acaggcaaca tgacggcggg agaacgggac cgcggacacc
      301 ttcggtatct gtccatggtc cttgtgtttc taaccatcct gtacgccctt ccccttttgg
      361 ctccagccga aacaaccggg acgctcaatg aagacctcgc ggccatcctc gacgaccccg
      421 cgctcgaagc ggcccacgtt ggggtcgtcg tcgactccct ggcaacggga cggaggatct
      481 tcgaatacaa cgccggcaag cggttcgttc ccgcatccaa catgaagctt ttcaccacgg
      541 ccgcggccct gttggcgctc tccccggatt tccggtggga gacgcggctt atcacggacg
      601 gcacggtgac ctccggcgtc ctgagcggag acatcatcgt cgagggatcg ggcgatccca
      661 ccatatcggg gtacttccac gagggagacg tgctccatgt gttccgccgg tgggcggcaa
      721 agctgaagga gcagggtgtg agggagatcg gcggcgacct tgtgatcgac aactccgcgt
      781 tcccggacaa gccgtacggc aaaggctggg acgtcgaccc ggtaaactgc ttcagcgccc
      841 caagggacgc cttcactttc aacaacaact gtattcagct cgacataacc ccatcgggca
      901 ggacaggaga ggcggcacgt atcgccatgg agcccgccac ggactacgtg agtctcgcgc
      961 gcggcctcac agtccgaaaa aatgcgggcc cggatgtcgt cagcctcgag tacgcgacgc
     1021 cgaggaccct gtcgataacc ggctccatcg ggcccggaac gaggatgata acccgctatg
     1081 tggccgtgaa ccatcccgcc tattttgggg gttttgtctt gagggaaacc cttgaagccg
     1141 ccggtatcgc cgtcaagggg gatatcctgt gcgcccgcaa ctgcccgaag acggtggacg
     1201 taagaacaag aagagagaag ggaaaatgga cgaccgttgc cgtctaccat tctccccggc
     1261 ttgccgagat cgtaaaggtg gtcaacaagc tcagcaacaa tctctacgcc gagcttctgc
     1321 tcctcgcgac gggcagggcc gcgggggcgg ccgggaccga ggggtccgcc gcggccgccc
     1381 ttgccgcgct tcgccgcgcc gggcttaata cctccggagt cgtcatggcc gatggctcgg
     1441 gtctctccag gcacgacctc gtcacgcccg acagcgtcgc gcggttattg aggatcatgg
     1501 ctcaggggcc ccacgcccgt tccttcatcg actcccttcc cgtcatgagc gtcgacggaa
     1561 ctcttggcgt gaggctcaag ggctcccgcg cggcggacag agtacgggca aaaacaggga
     1621 cgatgaccca cataaggggc ctttcgggat acgtgacgac gatgaaaggg gaaagactgg
     1681 tcttctccat cttctccaac aaccacgtcg cgacatcgga cgtcgacacc gccacggaca
     1741 ggatcattct caggctcctc gaccactcgt catcggaacg atagaaactc ctacctgatt
     1801 atcctccttc gcatcaacct gaacggaaag ggagacagca gaacaacgac cacgattatc
     1861 cagagtgcgc cctcaagggc gtcgccgggg gacccagcgg cgagggccct gcagacgttg
     1921 gtgaggtgat agagaggcgt gaaccaggcg accttgccga cgaaggcggg gagattgtgg
     1981 agaggaaaga atatgccgga caggagaaac atgggtgaca gaaaga
//
LOCUS       DAOPOA010000472         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_60247_length_2024_cov_3.373286, whole genome shotgun sequence.
ACCESSION   DAOPOA010000472 DAOPOA010000000
VERSION     DAOPOA010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60247_length_2024_cov_3.373286"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1891)
                     /locus_tag="PKJ17_09370"
     CDS             complement(<1..1891)
                     /locus_tag="PKJ17_09370"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOD76225.1"
                     /translation="MRVILAEKPSVAKDIANALGSAKSEKGYFKLPNGDYVTYAIGHL
                     VESDDTALTPQENGRAKYWNFEDLPIFPKQFIYKVSGDPGIAAQFRVVSKLVSSANEV
                     VIATDAGREGELIARHILAKSGFRGSVKRLWTSKALTPDVVRDEMKKLRPGSEFESLY
                     FAGLARDQADWLVGINLTRAVTLKSHHRMVWTIGRVQTPTLSLIVNRDLERQDFKPIP
                     YAVVKASFAKGAVSYDGTLLVRAVTQEDKEDDDTASRLSRERAEEIVGKLGPIPTGTI
                     AEVKKEDRRDSPPLLHSLTSLQREANSVYGYSAQETLNLTQSLYENHKAVSYPRTDAQ
                     HMSPSNKDLAKECLRKLGKQNLTIVVDKMGKRVFDDAKLTDHHAIIPFNKLPSSASRE
                     EANVYNLIYRKFVGAFMPDHLYIKTSILTDVSGLKFLTTGKEVKQAGWRALYTGDKAA
                     GEEQQLPSLAKGEKVNKTKVWKEDKETKPPAVHTEASILKLMEKLGLGTPATRASILE
                     RLKTTGYVVRDKKALTSTPKGRELIKRIKSGITSPEMTARWEQLLGEIYTKKQGKQGY
                     EGFLAGIRTFLSKEVTQIKGMQAIGMSLPPPAPKKPFSSSSGGSGKRPGGFGSKSRTG
                     FKKKSY"
BASE COUNT          331 a          594 c          571 g          528 t
ORIGIN      
        1 aatagctttt cttcttgaac cctgtcctgc tttttgaccc aaacccgccc ggtctcttgc
       61 cggagccgcc cgatgaggac gaaaagggtt tcttcggggc agggggcggg aggctcatgc
      121 ctatcgcctg catgcccttt atctgtgtga cttccttcga caggaaggtg cgtatgccgg
      181 cgaggaagcc ttcatacccc tgtttgcctt gtttcttcgt gtagatctcg ccgaggagct
      241 gctcccacct ggcggtcatc tccggcgatg tgatgccgga tttgatgcgc ttgatgagtt
      301 cgcggccctt cggtgtcgaa gtgagcgcct tcttgtcgcg gaccacatac ccggtcgtct
      361 tgagcctctc gaggatggac gccctggtgg ctggcgtgcc aaggcccagt ttctccataa
      421 gcttcaggat ggacgcctcc gtgtgcaccg ccggcggctt tgtttcctta tcctctttcc
      481 agaccttcgt cttgttgact ttctcacctt tcgcgaggga cggaagctgc tgttcttccc
      541 cggcagcctt gtcacccgtg tacagcgcgc gccaacccgc ctgcttgacc tccttccctg
      601 tcgtgaggaa cttgagtccg ctgacatccg tcaggatgct tgtcttgatg tacaggtgat
      661 ctggcatgaa ggcgccaacg aacttgcggt agataaggtt gtatacgttc gcttcctccc
      721 tgctcgctga agacggcagt ttgttgaatg ggatgatcgc gtggtggtcg gtgagttttg
      781 catcatcgaa tacgcgcttc cccatcttat ccacgacgat cgtcaggttc tgtttcccca
      841 gctttctgag gcattccttg gcgagatcct tgttggaagg ggacatgtgc tgtgcgtcag
      901 tacgcggata gctgacggcc ttgtggttct cgtacagact ctgtgtgagg ttaagcgttt
      961 cctgggcgga atacccgtag acggagttcg cctccctctg cagagatgtc agtgagtgga
     1021 gaaggggagg ggaatccctc ctgtcctcct tcttcacctc ggcgatggta cccgtcggaa
     1081 taggaccgag ctttccaacg atctcttccg ccctctcccg tgagagcctg ctggccgtgt
     1141 cgtcgtcctc cttgtcctcc tgggtcacgg cccgaacgag aagggtgccg tcataagaca
     1201 cggccccttt cgcaaaagag gcctttacga ccgcatacgg tatgggtttg aagtcctgcc
     1261 gctcgaggtc gcggttgact atgaggctca gtgtcggtgt ttgcactctc ccgatcgtcc
     1321 acaccatcct gtggtgtgac ttcagggtca cggcccgggt aaggttgatg cctacgagcc
     1381 agtctgcctg gtcacgcgcg agcccggcga agtagagtga ctcgaattcg ctgccgggcc
     1441 ggagcttttt catttcgtct ctcacaacgt ccggggtgag ggccttcgat gtccagagcc
     1501 gtttgacgga gccgcgaaaa ccgctttttg caaggatatg gcgggcgata agctcgccct
     1561 cacgccctgc gtcggtcgcg atcaccacct cgtttgcgga ggagacgagc ttgctgacga
     1621 ccctgaactg ggcagctatg ccagggtcgc cgctcacctt gtagatgaac tgtttcggaa
     1681 aaatggggag atcctcgaag ttccagtatt tcgccctgcc gttctcctgc ggtgtgagcg
     1741 cggtatcgtc ggattccacg aggtgcccga tcgcatacgt cacgtagtcg ccattgggaa
     1801 gcttgaaata gcctttttcg ctcttggctg accccagggc gtttgcgatg tcctttgcaa
     1861 ccgatggttt ttctgccagt atcactctca tgactctctc ctttgtgtct tgtgtcccct
     1921 gttaaatagc aggacgtttc acggtctcac cgttttctct cgccactgcc cccccatcgt
     1981 ccgacaacct accggaatca ttggcatatc accccgttgt cgga
//
LOCUS       DAOPOA010000473         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_60290_length_2023_cov_3.361280, whole genome shotgun sequence.
ACCESSION   DAOPOA010000473 DAOPOA010000000
VERSION     DAOPOA010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60290_length_2023_cov_3.361280"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKJ17_09375"
     CDS             <1..261
                     /locus_tag="PKJ17_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD76226.1"
                     /translation="KHALRNALLPVITILGLSIPGLIGGSVIFESIFSIPGMGQLFYF
                     SVMARDYPTIMGILVIGAFLTLVGNLVADVSYAVADPRIRIG"
     gene            403..1302
                     /locus_tag="PKJ17_09380"
     CDS             403..1302
                     /locus_tag="PKJ17_09380"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOD76227.1"
                     /translation="MKKHLKNLAPVARGLSTTAKKVFKKYQADHANIMVSSIAFYILL
                     TFIPFTLFSFFILGYVIDVGHTGVQMEKYIARAIPAPYNAVIVKKVLREINVISLSKR
                     LSGPLGLLFLLLFTSRLFAVLRPSFQLIFGKTKKGFIKGKQEEFVLTFAFALIQTIIF
                     FSYVFSVMMQVKVVKAFGGAVTRASVVQFFSVLDLFLVAGMFWFLYYVLTPVRDRRRL
                     LFVSALGAAMWHAGKYFFQYFVLYIGRFTTFFGTYGVFIAFLFWVYYSVFVFIVCAEL
                     LAVSSEPAVSGPVPNYARFRWKR"
     gene            complement(1256..>2023)
                     /locus_tag="PKJ17_09385"
     CDS             complement(1256..>2023)
                     /locus_tag="PKJ17_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOD76228.1"
                     /translation="PNLQNIPVRGQEGPKIREAFVPADGCLILSSDYSQIELRVLAHL
                     SGDRELIATFHRDEDIHSTVARDVFHVTADAMTQDMRRAAKVINFGIIYGMSAFGLAR
                     ELGVGQKEAQEYIDSYFERHRAVREFMDSVIEEARVKGFVRTLLGRMRFIPELGNSDP
                     AVRQLGERVAMNTPIQGTAADIIKMAMINIRRAIRLKGLSSRLILQIHDELLFEVTLD
                     ETETMKQLVRQEMEKVIELKVPLTVSIGIGHSWAQAH"
BASE COUNT          362 a          605 c          543 g          513 t
ORIGIN      
        1 aagcacgccc tgcgcaatgc cctcttgcct gtcatcacca tcctcgggct ttccataccc
       61 ggtctcatag gcggcagcgt catcttcgaa agcatctttt ccatccccgg catgggacag
      121 cttttctact tcagcgtcat ggccagggat taccccacga tcatggggat ccttgtcatc
      181 ggcgccttcc tgacgctcgt cggcaacctg gtcgcggacg tctcctacgc ggtggcggat
      241 ccaaggataa ggataggata aagacggttc caaggttcaa aagttcaagg gttcaaggat
      301 gaagagcctg attttctcga taggtcctat cggtcccata tgtcccatag gtcttataat
      361 tcctataggt cctattgctg ccccctgttc tcccggtgcg tcatgaagaa gcatcttaag
      421 aacctggcgc ccgtggcgag agggctttcc accacggcga agaaggtctt taagaaatac
      481 caggcggacc atgccaacat catggtctct tccatagcat tctacatcct gctgaccttc
      541 atacctttca cgctcttctc cttcttcatc ctcggatacg tcatcgatgt gggccatacc
      601 ggcgttcaga tggagaaata catcgcacgg gccataccgg caccctacaa cgcggtcatc
      661 gtcaagaagg ttctcaggga gataaacgtg atctcgttgt ccaagaggct ttcgggtcca
      721 ctgggcctcc tctttctcct tcttttcaca tcgaggctct ttgccgtcct gcgcccctct
      781 tttcagctca tattcggcaa gacgaagaaa gggttcataa aggggaagca ggaggagttc
      841 gtccttacct ttgcctttgc ccttatccag accatcatct ttttcagcta cgtcttttcc
      901 gtgatgatgc aggtgaaggt ggtgaaggcc ttcggggggg ccgtcacgcg cgcttcggtg
      961 gtgcagttct tttccgtcct cgatctcttt cttgtcgccg ggatgttctg gtttctctac
     1021 tatgtcctca cgcccgtgcg ggacaggagg aggcttctct tcgtctccgc cctgggggcc
     1081 gcgatgtggc acgcgggcaa gtactttttc cagtacttcg ttctctacat agggcgattc
     1141 acgaccttct tcggcaccta cggcgtcttc atcgcctttc tcttctgggt ctattactcc
     1201 gtttttgttt tcatcgtttg cgcggagctg ctggcggtct cctcggaacc ggccgtcagt
     1261 gggcctgtgc ccaactatgc ccgattccga tggaaacggt gagagggacc ttaagctcga
     1321 tcaccttttc catctcctgc ctgacgagct gtttcatcgt ttctgtctcg tcgagggtga
     1381 cctcgaagag cagttcgtca tgtatctgca ggataagccg cgaagagagg cctttgagcc
     1441 gtattgccct gcggatgttg atcatggcca tctttatgat gtccgcggcg gtgccctgaa
     1501 tgggagtgtt catcgcgaca cgctcgccga gctggcgaac ggccgggtcg gagttgccca
     1561 gctccggtat gaaccgcatc ctcccaagga gcgtcctcac gaaccccttc acccgggcct
     1621 cctcgataac gctgtccatg aactccctca cggccctgtg ccgctcaaag tagctgtcga
     1681 tgtactcctg ggcctccttc tggcccacgc cgagttcccg ggcgagaccg aaggcgctca
     1741 tgccgtagat tatcccgaag ttgatcacct tggcggcgcg ccgcatgtcc tgggtcatgg
     1801 cgtcggccgt cacgtgaaag acgtcccggg ccaccgttga atgtatgtcc tcgtcgcgat
     1861 gaaatgtggc gatgagttcc cggtcccctg acaggtgggc caggacgcga agctcgatct
     1921 gcgaatagtc cgaggacagg atcaagcatc cgtccgcggg cacgaaggcc tcgcggatct
     1981 tcggcccttc ctgtccccgt acggggatgt tctggaggtt ggg
//
LOCUS       DAOPOA010000474         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_60348_length_2022_cov_2.180478, whole genome shotgun sequence.
ACCESSION   DAOPOA010000474 DAOPOA010000000
VERSION     DAOPOA010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60348_length_2022_cov_2.180478"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PKJ17_09390"
     CDS             <1..1010
                     /locus_tag="PKJ17_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOD76229.1"
                     /translation="TAAQRTIEPCRRALDDAKIPTNKIDEVVLVGGSTRIPRVQDIVK
                     EFFGKEPHRGVNPDEVVALGAAVQAGVLAGEVKDVLLLDVTPLSLGIETLGGVLTRII
                     ERNTTIPTKKSQVFTTAEDSQTSVEIHVLQGEREMARDNRTLGRFHLIGLPPAPRGIP
                     QIEVTFDIDANGILHVSAKDTATGKEQSIQITASTGLSEDDIQKMVHDSETHAEEDRK
                     RKQEIEQRNQLDNIIYTTEKTLNENKDKLSAEDIRAVEDLLNPAKEAIKSGDAERIKK
                     AAEDLTRASHHLAETLYKKTSQATSQGAPGQDGGPQAEAQQGGRRDDDVVDAEFEDVK
                     R"
     gene            1020..>2022
                     /locus_tag="PKJ17_09395"
     CDS             1020..>2022
                     /locus_tag="PKJ17_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOD76230.1"
                     /translation="MVIGLKNDKSIKDRMFIPAELPVLPLRGTVAYPDLVMPLIVGRE
                     KSIRLVDEAMSKDKMIAIITQKNPDIEDPGPEDLYTVGTVATIMKMVKMVDGSQRVVI
                     QGLCRCKVIEFTEADPNIKASILPVFEDYQKDIEIDAMYINLKNLYRKAIEMAPYLSS
                     ELAQIASKVENPGNLADLIASTINISVIEKQEILEKIDLKERLKKATIYLNREVETLE
                     LSSKIQSHVKEGIDKTQREYYLREQLKAIQKELGESDDKINEVDELKKKIKEAKMPDE
                     VRKVADKELDRLSKMSSMSAEYTVARTYLDWLTELPWSKATEDNLDIDGATKILDEDH
                     "
BASE COUNT          570 a          555 c          602 g          295 t
ORIGIN      
        1 tcacggcggc ccagaggacc atcgagccct gcaggcgcgc ccttgatgat gccaagatcc
       61 cgacaaacaa gatcgatgag gtggtacttg tcggtggttc caccaggatc cccagagtcc
      121 aggatatagt caaggaattc ttcggcaagg aacctcaccg gggtgtgaac ccggacgagg
      181 tcgtggccct tggcgccgcc gtccaggcgg gcgttctggc cggcgaggtc aaggacgtcc
      241 ttcttctcga cgtcacaccg ctgtcactgg gcatcgagac gctcggcgga gtactgacga
      301 ggatcatcga acgcaacacc accatcccga ccaagaagag ccaggtattc accaccgccg
      361 aggacagcca gacgagcgtc gagatccacg ttcttcaggg cgagagggaa atggccaggg
      421 acaacaggac gctggggagg ttccacctca tcggcctgcc gccggcgccg aggggcatac
      481 cccagataga ggtcacgttc gatatagacg ccaacggcat actccacgta tcggcaaagg
      541 atacggcgac gggaaaggaa cagagcatac agatcaccgc ttccacgggc ctctccgaag
      601 acgatatcca gaagatggtc cacgattcgg agacccatgc cgaggaagac cggaagagaa
      661 aacaggagat cgagcagcgt aaccagctcg acaatatcat atacacgacc gagaaaacgc
      721 tcaacgagaa caaggacaag ctctccgccg aggacatcag ggccgtcgag gacctgttga
      781 accccgcgaa ggaagccatc aagagcggcg atgccgagag gataaaaaaa gcggcggaag
      841 acctcaccag agcctcccat catcttgcgg agacactcta caagaaaacg tcgcaggcaa
      901 cgtcgcaggg cgctccggga caggatggcg gcccgcaggc tgaggcccag cagggcggca
      961 gaagggatga cgacgtcgtg gacgcggaat ttgaggatgt caagcgatag ggggaattaa
     1021 tggtaatcgg cttaaagaac gacaaaagca taaaagacag gatgttcata ccggcggaac
     1081 tgcccgttct gccgctgagg ggtacggtgg cgtaccccga tcttgtcatg cccctcatcg
     1141 tggggcgcga gaaatcgata cgcctcgtcg atgaagcgat gagcaaggac aagatgatag
     1201 ccatcataac gcagaagaat cccgatatcg aggacccggg cccggaagac ctctataccg
     1261 tcgggaccgt ggccaccatc atgaagatgg tcaagatggt cgatggcagc cagagggtcg
     1321 tgatccaggg cctgtgcagg tgcaaggtga tagagttcac cgaggcggat cccaacatca
     1381 aggcaagcat cctccctgtc ttcgaggact accagaagga catcgagata gatgccatgt
     1441 acatcaacct caagaacctc taccgcaagg ccatcgaaat ggccccgtac ctttcatcgg
     1501 aacttgccca gatagcgtcg aaggtggaga acccgggcaa cctggccgac ctcatcgcct
     1561 cgacgataaa catatcggtc atcgaaaagc aggagatcct ggaaaaaatt gacctcaagg
     1621 agcgcctgaa gaaggcgacg atctacctca acagggaagt ggagacgctg gagctcagca
     1681 gcaagatcca gtcacacgtc aaggagggca tcgacaagac ccagcgcgag tactacctga
     1741 gggaacagct caaagccatc cagaaggagc ttggcgaaag tgacgacaag ataaacgagg
     1801 tcgatgagct caagaagaag ataaaagaag ccaaaatgcc cgatgaggtg cggaaggtgg
     1861 cggacaagga actcgacagg ctctcgaaga tgagcagcat gtccgctgaa tacaccgtcg
     1921 cgcggacata cctcgactgg ctgacggagt tgccctggtc aaaggcgaca gaggacaacc
     1981 tcgacattga cggggcaacg aaaatactcg acgaagacca ct
//
LOCUS       DAOPOA010000475         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_60483_length_2018_cov_3.107489, whole genome shotgun sequence.
ACCESSION   DAOPOA010000475 DAOPOA010000000
VERSION     DAOPOA010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60483_length_2018_cov_3.107489"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKJ17_09400"
     CDS             <1..295
                     /locus_tag="PKJ17_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glutaconate CoA-transferase"
                     /protein_id="HOD76231.1"
                     /translation="RTDPDQNSLPPFFVDAIVKVPYGAHPTACYGFYDYDPKHLNLYR
                     KMAEDDTLFTEYLDEWVYGVGSWEDYLTKVGMEMILKIKANPVIGYAPGLDRK"
     gene            308..1093
                     /locus_tag="PKJ17_09405"
     CDS             308..1093
                     /locus_tag="PKJ17_09405"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOD76232.1"
                     /translation="MIRYSDNEMMALAAGRFIKDGDIVFAGTGVSILAATAAKRIFAP
                     KAVIFFETGGIDPSLDEIPMAVADLRVMAGTSLNSGLIEAFSIVGHRKLHTVAFLGAA
                     QIDRYGNLNTTCIGDYFRPKTRFSGSGGACDVATFASGVITFMQHEKRRFVEKLDYLT
                     SVGWYEGGDSRKELGLRRGGALAVVTNLGVLRFHEETKEMYLAEYFPGVTIEKIVEST
                     GFPIDTSMAVEAETPTPGELQILREEVDPQRLICQAVLETPQK"
     gene            1326..>2018
                     /gene="tig"
                     /locus_tag="PKJ17_09410"
     CDS             1326..>2018
                     /gene="tig"
                     /locus_tag="PKJ17_09410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOD76233.1"
                     /translation="MKVQVEALDSVRKKVEVLLPRETVSGIRESIYDEIRRSAKIKGF
                     RQGKVPRPIITQYYKEYIEEETRKRMLQNTMFDALKEANVEPITEPVADFLEGDEPGY
                     TLDCEVLPEIELPEYKGIEIEAEPINISDADVEQRIESMRQFHASFEAKQGEATAQKG
                     DFVVIKYQGYRDGEALKQIAADGYPLELGNAMLMPEFENAVIGMKEGEERDVDVTFPD
                     DYPDKDIASKKIL"
BASE COUNT          489 a          510 c          619 g          400 t
ORIGIN      
        1 caggacggac cccgatcaga actcgttgcc tccctttttc gtggatgcca tagtcaaggt
       61 cccctacggt gcccatccga cggcatgcta cggcttttat gactacgacc cgaaacatct
      121 taacctctat cgcaagatgg ccgaggatga taccctcttc acggagtatc ttgacgaatg
      181 ggtctacggg gtcggctcct gggaagacta tctcacgaag gtgggaatgg agatgatcct
      241 caagatcaag gccaatccgg tgatcgggta cgcgccggga ctcgacagga aataaggagg
      301 ttgtctcatg atccggtaca gtgacaatga aatgatggcc ctggcggcgg gacggttcat
      361 aaaggacggc gacatagtgt tcgcgggtac gggcgtgtcc atactggcgg ctaccgccgc
      421 gaagaggatc tttgctccga aagcggtgat attcttcgag acgggaggca tcgacccgtc
      481 tctcgacgag ataccgatgg ccgtggcgga cttaagagtg atggccggta cgtcgctcaa
      541 ttccgggctc atagaggcct tctccatagt gggccatcgc aagctccaca cggtcgcctt
      601 cctgggggcc gcccagatag accggtacgg aaacctcaac acgacgtgca tcggtgatta
      661 cttcaggccg aagacccgct tttccggaag cgggggtgcc tgtgacgtgg cgacattcgc
      721 atccggggtt atcacattca tgcagcacga gaaacggagg tttgtcgaga aactcgatta
      781 tctcaccagt gtgggctggt acgagggcgg ggattcaagg aaggaactcg gcctgaggcg
      841 cgggggcgcc ctggccgtcg tcaccaatct cggcgtgctc aggtttcacg aggagacgaa
      901 agagatgtac ctcgcggaat atttcccggg tgtaacgata gagaagatag tggagagcac
      961 cggtttcccc atagacacgt ccatggctgt ggaggcggaa acccccaccc cgggagaact
     1021 ccagatactg cgcgaggagg tggaccccca gcgcctgatc tgccaggccg tcctcgagac
     1081 accgcagaag tgaagactgc ttgaatcgct tgagtggcct gaggggagga taggcaggat
     1141 cccggttcgc atcgagggat cgagaccccg cggatggtac gcaagacccg ggttgtccca
     1201 acattcaagc gattcaagcc attcaagcga ttcaagccat tcttattgct aatttcgcaa
     1261 aattaggttt ataatagtgc agttttctag ggactttgaa gtcccttttt catgtcggag
     1321 gtgttatgaa ggttcaggtt gaagcgctcg acagcgtgag aaagaaagtt gaagttcttc
     1381 ttccccggga gaccgtctca ggtataaggg aatccatcta cgatgagata agaaggagtg
     1441 cgaagatcaa agggttcagg cagggaaagg tcccgagacc catcatcacc cagtattaca
     1501 aggaatatat cgaggaagag acgcggaaga ggatgcttca gaacaccatg ttcgacgctc
     1561 tgaaagaggc gaatgtggag ccgatcacag agcccgtggc ggattttctc gaaggtgacg
     1621 agccaggcta cacgcttgac tgcgaggttc ttcccgagat cgagctgccc gagtacaagg
     1681 gtatagagat cgaggcggag cccatcaaca tatccgatgc ggatgtggag cagcgtattg
     1741 agagtatgcg ccagttccat gccagcttcg aggcgaagca aggcgaggcg acggctcaga
     1801 agggcgattt tgtcgtcatc aagtatcagg gataccgcga cggcgaggcc ttgaaacaga
     1861 tcgcggccga cggatatccc ctggagctcg ggaacgccat gctcatgccc gagttcgaaa
     1921 atgccgtcat cggcatgaaa gagggcgagg agagggacgt ggacgtgacc ttcccagacg
     1981 attatcccga caaggacatc gcgtcgaaga agatcctt
//
LOCUS       DAOPOA010000476         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_61006_length_2004_cov_2.563879, whole genome shotgun sequence.
ACCESSION   DAOPOA010000476 DAOPOA010000000
VERSION     DAOPOA010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61006_length_2004_cov_2.563879"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /gene="fliF"
                     /locus_tag="PKJ17_09415"
     CDS             complement(<1..1332)
                     /gene="fliF"
                     /locus_tag="PKJ17_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOD76234.1"
                     /translation="MPPMDELLNTTKNYLKTTPKSKLYMYLFLFIALVGGSIIGLSFL
                     QKETYQTLFAGLSTEDASVVVAKLKELKVPYKLGLGGTSISVPKDKVYDVRLMLAGQN
                     ALPGGGGVGYELFDKTNYGMTEFMQNINYKRAIQGELSRTINQMPEVKASRVHIAIPE
                     KTLFTDREKDVTASVFLKLRGGKTLSKEQVAGIVHLVSGSIEGLKADNVTIVDSFGKI
                     LYKAGSSGTAVALSGQQFELQKSVERTIEESVQSMLDRFIPANKSIIRANVDLNLRKV
                     EKVEEEFDPNKRVPTSERKSREKTLNRTAGQAGVPGVASNVPNATGKDRNASAAVSQT
                     SAGRGSESEREETQVTYEVSKTVKKIVEPYGDIKRLSVAIVLDGKYEKVKGQNGEELK
                     YSPRSQKELTDIKGIVARAVGIDEARGDKIEVLNVPFETENLADEKGLLDAA"
     gene            complement(1336..1623)
                     /gene="fliE"
                     /locus_tag="PKJ17_09420"
     CDS             complement(1336..1623)
                     /gene="fliE"
                     /locus_tag="PKJ17_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOD76235.1"
                     /translation="MKVENFTLPSFGEVVRPANEKTRTSFGDVLKDSIKKVVELEKEA
                     DNEVEKLVKMENTDVQTTMIAIEKADLSFQMMMQIRNKIISAYEEIMRMQV"
     gene            complement(1636..>2004)
                     /gene="flgC"
                     /locus_tag="PKJ17_09425"
     CDS             complement(1636..>2004)
                     /gene="flgC"
                     /locus_tag="PKJ17_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOD76236.1"
                     /translation="DIAASNLANVQTTNTEEGGPYRKKDVVFKAADVSDDKSFSGTLS
                     KKVEGVEIDSVKESDKPFQLVYNPSHPDANSEGYVTFPNVNLMEEMTDMTAAARAYEA
                     NVNVLNTTKDMFLKTLDLIK"
BASE COUNT          338 a          589 c          550 g          527 t
ORIGIN      
        1 cgccgcgtcg agaagacctt tctcgtcggc gaggttttcg gtctcaaagg gaacgttcaa
       61 gacctcgatc ttgtcgcccc tcgcctcgtc tatccccact gccctcgcca cgatcccctt
      121 gatgtcggtg agctctttct gtgaccgcgg ggaatacttg agctcctcac cgttctggcc
      181 cttcaccttt tcatatttcc cgtcgagaac gatggcgacg gagaggcgct tgatatcgcc
      241 gtagggctcc actatcttct tgacggtctt gctcacctcg taggtaacct gggtctcttc
      301 gcgttccgat tcactgccgc gccctgccga cgtctgggac acggccgccg acgcgttccg
      361 gtccttgccc gtggcgttgg gaacgttgga tgccaccccg gggactcctg cctgcccggc
      421 cgtgcggttc agtgtcttct ccctgctctt gcgctcgctc gtggggaccc tcttgttcgg
      481 gtcgaactcc tcttccacct tttcaacctt gcggagattg agatccacat tggcccgtat
      541 gatcgacttg ttggcgggga tgaacctgtc gagcatggat tggaccgatt cttcgatggt
      601 cctttcaacg ctcttctgca gctcgaactg ctggcccgaa agggcgaccg ccgtgccgga
      661 gctcccggcc ttgtacagta ttttgccgaa ggaatcgacg atcgtcacat tgtcggcctt
      721 gagcccctct atgctgcccg aaaccaggtg gacgatcccc gccacctgtt ccttggagag
      781 cgtcttcccg ccccggagct tgaggaagac cgacgcggtg acgtcctttt ccctgtctgt
      841 gaagagggtc ttttccggta tggcgatgtg cacccgggat gccttcacct cgggcatctg
      901 gttgatggtg cgcgaaagct ccccctgtat ggcgcgtttg taattgatgt tctgcatgaa
      961 ttccgtcatg ccgtagttcg tcttgtcgaa gagctcgtaa ccgacccctc cgccgccggg
     1021 aagggcgttc tgaccggcaa gcatgagccg cacgtcatag accttgtctt tggggaccga
     1081 gatcgaggtg ccgccaagac ccagcttgta gggaaccttg agctccttga gcttcgccac
     1141 gaccaccgag gcgtcctccg tggagaggcc ggcgaacagc gtctggtagg tctctttctg
     1201 taggaaggaa agaccgatga tggagcctcc cacgagcgct atgaaaagaa agaggtacat
     1261 gtagagtttg ctcttcgggg tcgttttgag atagttcttg gtggtgttga gaagttcgtc
     1321 catcggaggc atacgttaca cctgcattct cattatctct tcataagcgg agatgatctt
     1381 gttccgtatc tgcatcatca tctggaagga caggtccgct ttctcgatgg cgatcatcgt
     1441 ggtctgaaca tcggtgttct ccatcttgac cagtttttcc acttcgttgt ccgcttcctt
     1501 ctcgagttcc acgaccttct tgatggagtc cttcaggaca tcaccgaagg aggtcctggt
     1561 cttttcgttg gccggtctta cgacctcgcc aaaactggga agagtaaagt tttctacctt
     1621 catgggtccg cccccctatt taataaggtc gagagtcttc aggaacatgt ccttcgtcgt
     1681 attgagaacg ttgacgttcg cctcgtaggc acgggcggcc gccgtcatgt ccgtcatctc
     1741 ttccatcagg ttcacgttgg gaaatgtgac gtatccttca gaattggcgt cgggatggct
     1801 cgggttgtag accaactgaa atggcttgtc gctctccttg acagagtcga tctcgacgcc
     1861 ctccaccttc tttgagaggg tccccgagaa gcttttgtcg tcggagacgt ccgccgcctt
     1921 aaatacaaca tccttcttcc tgtagggccc gccctcttcc gtgttcgtgg tctggacgtt
     1981 ggcaaggttc gaggccgcga tgtc
//
LOCUS       DAOPOA010000477         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_61008_length_2004_cov_2.539251, whole genome shotgun sequence.
ACCESSION   DAOPOA010000477 DAOPOA010000000
VERSION     DAOPOA010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61008_length_2004_cov_2.539251"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PKJ17_09430"
     CDS             <1..863
                     /locus_tag="PKJ17_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOD76237.1"
                     /translation="EIHDRYRKDRKGKGSFDRAMTGLKLLQKHGVEYNVLAVVARETA
                     RAPFEVYNFFEGEGIEFVQFSPVVERLSGPKGRRMGLKLAGPAPLNKATEEVEAAPWS
                     VIPGEYGDFLIAIYEKWVRRDVGRVFIMNFEWALNAWIGNPSPVCVHAGQCGRSLVIE
                     HSGDVYACDHCVYPEYRLGNVATDRLSAMVERSLESGFGIAKETALPRWCRECDVLAA
                     CRGGCPKHRFATTWYGESGLHYLCEGYKKFFLHIRKYLRAMATLLENGLPASRVMEAI
                     KGPLVIRPGT"
     gene            922..1464
                     /locus_tag="PKJ17_09435"
     CDS             922..1464
                     /locus_tag="PKJ17_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOD76238.1"
                     /translation="MTETCNNDGKSGIITDYEVHGSARSDIGKALDVIERLDSAGLKP
                     ETLFADGGYPSVPSALKIAEKGIEFMTPVNRSRMGNNVIGRDLFVFDPDGRVRERPMG
                     HAPIDRRVLSGNNTTHRSLRAIFDGDLCRSCTMLDHCPVRVPNHRDRGRGALDTVVDF
                     RLVKVCFSVACKVIACNIKR"
     assembly_gap    1526..1535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1801..>2004)
                     /locus_tag="PKJ17_09440"
     CDS             complement(1801..>2004)
                     /locus_tag="PKJ17_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76239.1"
                     /translation="EVLRIFREQPNRFHLVITNYAMPDMNGLDLAKRLLKVRADIPIF
                     SVRDTTTTSLRKRSRKPGSGNSS"
BASE COUNT          416 a          525 c          600 g          453 t
ORIGIN      
        1 aagagatcca cgaccggtat cgtaaggacc ggaaaggcaa aggcagcttc gatcgggcca
       61 tgacgggcct gaaactcctg cagaaacatg gcgtggaata caatgtccta gcagtcgttg
      121 ctagggagac ggcgcgtgcg cccttcgagg tgtataattt cttcgagggg gagggcatcg
      181 aattcgtcca attctccccg gttgtcgagc gcctgtcagg cccgaagggc aggcggatgg
      241 gactgaagct cgccggcccc gcccccctta acaaagcgac agaagaggtc gaagccgccc
      301 cgtggtcggt cattcccggg gagtacggcg attttctcat cgctatttac gagaaatggg
      361 tgcgccgtga tgtgggacgc gtttttataa tgaacttcga atgggctctc aacgcttgga
      421 tcggcaaccc gtcaccggtt tgcgttcatg ccgggcagtg cggccggtca ctcgtcatcg
      481 agcactcagg cgacgtgtat gcctgtgatc attgcgtcta ccccgagtac aggctcggca
      541 acgtcgcaac cgacaggctg tcagccatgg tggagaggtc gcttgaatcg ggatttggca
      601 tcgccaaaga aacggcattg ccgcgctggt gccgggaatg tgatgtcctt gccgcatgcc
      661 ggggcggatg ccccaagcat cgttttgcga caacttggta cggcgagtcc gggctccact
      721 atctgtgcga ggggtataaa aagttctttc tccatatccg gaagtacctg agggccatgg
      781 ccacgctgct cgaaaacggg ctgcccgctt ctcgggtcat ggaagccatt aagggaccgc
      841 tcgtcatcag gcccggcacg tagaacacgg cgtgcagggt gcgcatcctt cgggcataaa
      901 ggggcgggat atagcgtcca tataaccgag acctgcaaca acgacggcaa atccgggatc
      961 atcaccgatt acgaggtgca cggctccgcc cggtcggata tcggcaaggc cctcgacgtc
     1021 atcgaacgcc tcgacagtgc cggattgaaa ccggagacgc tctttgccga cggcgggtac
     1081 ccttcggtcc cgtccgcgtt gaagatcgcg gagaagggga tagagttcat gacacctgtc
     1141 aatcgcagca ggatgggtaa caatgtcatc ggcagggatc tctttgtctt tgatccggat
     1201 ggccgtgtaa gagagcgccc catgggccat gctcctatag accgtcgcgt actcagcggg
     1261 aacaacacca cgcaccgttc gcttcgtgcc atattcgacg gcgatttgtg ccggtcatgc
     1321 accatgctcg atcactgtcc tgtcagggtg cccaaccatc gtgacagggg acgcggggcg
     1381 cttgatactg tagtggactt ccgcctggta aaggtctgct tctctgtggc atgcaaggta
     1441 atagcgtgca acataaagag gtgagccctg gcccacggga ttctggaacc gttcttcgaa
     1501 tacgatatga atgctatctt cacttnnnnn nnnnncctgt gcgatggtga tatcggcaac
     1561 gtgtccgtct ctgtcgaggt ggttgtggac ggaacaggaa atctgatcct tgaaaaaggc
     1621 ggttgcttct taggaatcac atttttaagg acgtcaccga cttctgttag atatagagtt
     1681 tgtgagcgtt aaacgttagg tgtggttgtg cggggatgtc gcgattctgt caggtcgaaa
     1741 cggtagtgct gtccaatctc ctgcggatgg cccggttcag gtcctgtttc gactgaggtt
     1801 ttaagaggaa ttccctgatc ccggctttct tgaccttttc cggagagatg tcgtcgttgt
     1861 atcccgtaca gagaatatgg ggatatccgc gcgtaccttg agcagcctct ttgcgagatc
     1921 gaggccgttc atgtccggca tcgcataatt cgtgatcacg agatgaaacc tgttgggttg
     1981 ttccctgaag atcctcagca cttc
//
LOCUS       DAOPOA010000478         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_61209_length_1998_cov_3.868760, whole genome shotgun sequence.
ACCESSION   DAOPOA010000478 DAOPOA010000000
VERSION     DAOPOA010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61209_length_1998_cov_3.868760"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1996
                     /locus_tag="PKJ17_09445"
     CDS             <1..1996
                     /locus_tag="PKJ17_09445"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD76240.1"
                     /translation="KGISKALYKSGRRHLFVCGEMGTGKTLISLATAGNAPIMRVLVV
                     CPTHLVEKWIREAQRSIPEVHTVDLTVKNVISVLDGFRHERTVLNRHEVHVISKEKAK
                     LSYAWRPAVLMTKRSIFPHCPSCGQITMKDDEYLTFEDLSRRKVFCRSCGNPLWQADR
                     SLRRFAPAEYIKKYLKGFYDMVIIDEIQDYKAEGTLQGRAMGALLSASRFALCLTGTL
                     NSGYADDIFHLLFRMEPARLKADGFSHEDSNKFLSTYGTIEVVRKLDEVDNVYGRGKK
                     KAEMIRRRPGVSPVAIGKYLLDKTVFIRLADVIDGLPPYEENVLSYSMEERQTEAYED
                     LQERLKDAVKTYKTKALGSMLQALLSYPDSCVQFPENIIIRDKGADEVLETIEAPILR
                     FESGELLPKEKDFVGIVKKERDEGRKVLCYLTFTNSRDIRPRLKNILEEEKIRVGVLE
                     SSVSPKRREEWVRKHSPGFDVLLTNAELVKTGLDLCDYPTIYSFQTGYNIFTLRQAAR
                     RSWRIGQTVPVRVYFAVYRNTMQEIALSLVAKKLEVALMVEGELPEGLADYASSGGSI
                     VQELGKALIEGGDYAGAERAWANFRKKEVEAQLGVAGRETVFYEKRNGTSSAQTAIQD
                     NVTIKVSFIEGKRKSRSVVEINADDLETVAAGRMVQFALF"
BASE COUNT          511 a          527 c          560 g          400 t
ORIGIN      
        1 aaagggaatt tcgaaggcac tctacaagag cgggagaagg catctattcg tgtgcgggga
       61 aatgggaacc ggcaaaacgc tcatttccct ggcaacagca ggaaatgccc ccatcatgcg
      121 ggtcctcgtc gtctgtccca cacatctcgt ggagaaatgg atacgggagg cccaacgctc
      181 gattcccgag gtacacacgg ttgatcttac agtaaagaac gtaatttcag tccttgacgg
      241 cttccgtcac gagcggactg tcctcaacag acatgaagtc cacgtaatct caaaagagaa
      301 agcgaagctc tcctatgcgt ggcggcctgc ggtactaatg acgaagagga gcatctttcc
      361 ccactgcccg tcatgtgggc agatcaccat gaaggacgac gaatatctaa ccttcgagga
      421 cttatcgaga agaaaggtct tctgccgatc ctgcggcaac cccctctggc aggccgacag
      481 gtcgctgaga aggttcgcgc ccgccgaata catcaagaag tatctcaagg gcttctatga
      541 catggtcatc atcgacgaaa tccaggatta caaagccgaa ggaaccttgc agggccgggc
      601 aatgggcgct cttttgagcg cttcgcggtt cgcgctgtgt cttacgggca cgttgaactc
      661 gggttacgcc gatgacatct tccacctcct tttcaggatg gagcccgcga ggctgaaggc
      721 cgacggcttc agccatgagg acagcaacaa gttcctttcc acctatggga ccatcgaggt
      781 ggtgaggaag ctggatgagg tggataacgt ctacggcaga ggaaaaaaga aggcggagat
      841 gatccggagg agaccgggtg tatcgccagt agctatcgga aaatacctgc tcgacaagac
      901 cgtcttcatc cgccttgccg atgtgatcga cggcctgcct ccctacgaag agaacgtact
      961 ctcttattcc atggaggaaa ggcaaaccga agcctatgaa gacttgcagg aacgcctgaa
     1021 ggacgccgta aagacctaca agacgaaggc tctcggatcg atgttgcagg ccttgctttc
     1081 ctatccagat tcctgtgtcc agtttccgga gaatatcatc atacgggaca aaggagcgga
     1141 cgaggtgctg gagacgattg aggccccgat tcttcggttc gaatcaggcg aacttctccc
     1201 gaaagagaag gattttgtgg gtattgtcaa aaaggagcgc gatgagggcc gaaaggtgct
     1261 ctgttacctt accttcacca acagccgcga catacggccc cggctcaaga acatactcga
     1321 agaggagaag atcagggtcg gtgttctcga atcatccgtt tccccgaaac gccgggaaga
     1381 atgggtccgg aaacactcac ccggcttcga tgttctcctc acgaacgcgg aactcgtgaa
     1441 gacgggtctc gatctctgcg actacccgac gatctattca tttcaaacag gatataacat
     1501 cttcaccctc cggcaggctg cgcgaagatc ctggcgcatc ggccagacag tgccggtccg
     1561 ggtctatttc gcggtctacc ggaacaccat gcaggagatt gccttatccc tcgttgccaa
     1621 gaagctcgaa gtagcactta tggtggaagg agaacttccg gaaggtctcg cggattacgc
     1681 ctcgtcggga ggttccatcg tccaggaact tggcaaagcc ctcatcgagg gtggtgacta
     1741 tgcaggcgcc gagcgggcct gggcaaactt cagaaagaag gaagtcgagg cgcaacttgg
     1801 cgtagccgga agagagaccg ttttctatga aaagaggaac ggaacatcct ccgcgcagac
     1861 cgctattcaa gacaacgtca ccataaaggt ttcgtttatc gagggaaaaa gaaaatcgag
     1921 atccgtcgtg gaaatcaacg ccgatgacct cgagaccgta gcggcgggca gaatggttca
     1981 gttcgccctc ttctgagc
//
LOCUS       DAOPOA010000479         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_61258_length_1997_cov_2.804325, whole genome shotgun sequence.
ACCESSION   DAOPOA010000479 DAOPOA010000000
VERSION     DAOPOA010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61258_length_1997_cov_2.804325"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..513
                     /locus_tag="PKJ17_09450"
     CDS             7..513
                     /locus_tag="PKJ17_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76241.1"
                     /translation="MDSLSKTEYKHFLGTLKKAHAGISRSMMLFRNLLDKNIEKVLGI
                     TPTSIEWNITVTEPSHPDIAFVHPFDFHFDLLWFLIPMCILRKPFESHFRKLIPKTVE
                     AHLSRLAYQWEIRINKTIDEVRDQAMEYVKDELSTIDALLSGMQSRTGSTSTMMEELK
                     QTLDQTMQ"
     gene            959..1789
                     /locus_tag="PKJ17_09455"
     CDS             959..1789
                     /locus_tag="PKJ17_09455"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOD76242.1"
                     /translation="MDTEGNALSGNKEQVTNHNPLAVFSGGAVIGILGGLIGLGGAEF
                     RLPLLIGAFRFAALEAVILNKAMSLVVVASALPFRAATVPFSSVFGRYDILITLLSGS
                     LAGAWLGAGWAIRLRSQRLYRVIAILLIGIAAVLLFGHGLRDGSAPLFHGWLLTCVGV
                     VAGIGIGIVAALLGVAGGELLIPTIMLLFGADLKLAGSIALAVSLPTMITGFARYSRD
                     SSFAVIRTQWKFITIMAVGSLVGSYLGGHLLGIVPTPVLLPLLALILVISAIKVWRHK
                     "
BASE COUNT          421 a          640 c          506 g          430 t
ORIGIN      
        1 taagagatgg actccctgtc gaaaaccgag tacaagcact tcctgggcac cctaaaaaag
       61 gcccatgcag gcatctcccg ctcgatgatg ctctttcgga acctcctcga caagaacatc
      121 gaaaaggtgc tgggcatcac cccgacaagc atcgaatgga acataaccgt cacagagccc
      181 tcccaccccg acatagcctt cgtccatccc ttcgacttcc acttcgacct cctctggttc
      241 ctcatcccca tgtgcatcct tcgaaagccg ttcgagagcc attttcgcaa gctcatcccc
      301 aaaaccgtcg aagcccacct ctcccgcctg gcataccaat gggaaatacg catcaacaag
      361 actatcgacg aggtccgcga ccaggcaatg gaatacgtca aggacgagct ctcaacgatc
      421 gatgccctgc tatcgggcat gcaatcccga acaggctcaa cctccaccat gatggaagaa
      481 ctgaaacaaa ccctggacca aacaatgcaa tagaacaaac aacgcggccg gtcaggttgt
      541 tctcgtatcg tgaggtccgc gctgggtgcc tccaccctga tcaagtcatc ttaattcatc
      601 tgaaggtggc ggtgcctcgg caagctcctc gggttctgcg gtgtgatcct taagcgtggc
      661 tgcacggcaa agagacctca ggcgaccact atccggaggt attccctgag aggtttgtgc
      721 ttcaatagac ttttccgtga tggtgactcc ctgatgatgt ttgcgtttct ccaaatgtac
      781 aatgcgagta tattatgaga aacgatgaga tcgcatagag agcagggagg tggccggatc
      841 cacaaacctc ccgtccttgt tccatcgctc acatcacctt tcaacggttg caccccgccc
      901 gccttatatg atacggtaca tagcggtaac acgaacctag tcagcgaggt acgaaagaat
      961 ggacacagaa gggaatgcac tctccgggaa caaggagcag gtcaccaatc acaatcccct
     1021 tgccgtattc agcggcggag cggtcatagg gatccttggc ggcctcatcg gccttggagg
     1081 ggcggagttt cgtctcccct tgctcattgg ggcctttcgc ttcgccgctc tcgaagccgt
     1141 catcctcaac aaggccatga gccttgtggt cgtcgcctcc gccctgcctt tccgcgctgc
     1201 caccgttccc ttctcgagtg ttttcgggcg ctacgatatc ctcatcaccc ttctttcggg
     1261 cagccttgcc ggcgcctggc tgggggccgg ctgggccata cgcctgaggt cccagagact
     1321 ttaccgtgtc atcgccatcc ttctcatcgg gatcgccgcg gtcctcctct tcggacacgg
     1381 cttaagagac ggcagcgcgc ccctcttcca tggctggctc ctcacatgcg tgggcgtcgt
     1441 tgccgggata ggcatcggga tcgtagccgc attgctgggt gtggcaggcg gagaactcct
     1501 catcccgacg ataatgctgc tcttcggggc cgatctgaag ctcgcgggga gcattgcact
     1561 ggcagtgagc ctgcccacca tgatcaccgg ctttgcccgt tacagccggg acagcagttt
     1621 cgcggtgatc cgcacccaat ggaagttcat aaccataatg gccgtcggtt ccctcgtcgg
     1681 ctcctacctg ggaggccacc tcctcggcat cgtcccaacc cccgtcctcc tccccctcct
     1741 cgcgctgata ctggtgatct ccgcgatcaa ggtctggcgc cacaagtaac ggcaatgaac
     1801 gggaaccatc tgatctgcag cttgcggggt gtggacgaga acccgcacag gtgtctcagt
     1861 ccccggaatc tccgggctgg tggcgcaatt tgaagaggaa ttcgccaata aggtcttgtt
     1921 gcatttacac gaaaaaccta ctgtccctta tccccgtcaa ccaccctccg caccgccgac
     1981 gccagttcct gtctctt
//
LOCUS       DAOPOA010000480         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_61407_length_1993_cov_3.018576, whole genome shotgun sequence.
ACCESSION   DAOPOA010000480 DAOPOA010000000
VERSION     DAOPOA010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61407_length_1993_cov_3.018576"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PKJ17_09460"
     CDS             complement(<1..799)
                     /locus_tag="PKJ17_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOD76243.1"
                     /translation="MRSRNTFPGRFFRRIGNVFRRFAFLIMTAWATLAVYYSDLPPSF
                     RLIAAGLFVIGGLAVLTVGYRRRAARFVFLAAFAAVLVWWLLIPPSNTRNWQPDVALL
                     PWAAIDGDRVTIHGIRNVDYRTETDYTVRHYDRTFDLTKMKSVDLFVVYWGSPLIAHT
                     MLSFGFAGGDQVCFSIETRKEVGEEYSSIKGFFKQYELTYIIGDERDLVRLRTNYRKE
                     QVYLWRLRTSMDLARNVFLDYLRTVNLLRDRPEWYNALTSNCTTNIRG"
     gene            complement(882..1466)
                     /locus_tag="PKJ17_09465"
     CDS             complement(882..1466)
                     /locus_tag="PKJ17_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76244.1"
                     /translation="MKRTLSVVLASLLVVFIASVGFSEEKKAAKKAGPKPAGAMVDVI
                     EMTAVIDAVDAAKRTVTFKMPDGTARAFKVGPEVKNFDQIKAGDKVKTTFYESIAVFV
                     AKTGEKPSATEFGTVEVAPKGAKPGAVVVDTLEVTAKVEKIDYKKRTISLKGPEGNVR
                     TFTVDKSVKNFKNIKKGDDVYLRVTEAVAMVVEK"
     gene            complement(1630..>1993)
                     /locus_tag="PKJ17_09470"
     CDS             complement(1630..>1993)
                     /locus_tag="PKJ17_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76245.1"
                     /translation="MGKDPGELYGELADRLGRPLYVRVDAPATAAERAAVSSLKPEAI
                     EDEELAGDPITSKMTKAPGNNAAIGGVKVTTARGWFAVRPSGTEDIIKMYAESFVDEA
                     HLDSILAGARRTVRNALA"
BASE COUNT          387 a          620 c          560 g          426 t
ORIGIN      
        1 gaccccggat gttggtcgtg cagttgcttg tgagggcgtt gtaccattcg ggacggtccc
       61 gcaggaggtt cacggtgcgc aggtaatcga gaaagacgtt gcgcgcgaga tccatcgatg
      121 tcctcaggcg ccacaggtag acctgttcct tgcggtagtt cgtcctcaga cgaaccaggt
      181 cacgctcgtc gccgatgatg taggtaagct catactgttt gaagaaccct tttatggaag
      241 agtactcctc tccgacctcc ttgcgcgtct cgatggagaa acagacctga tcccccccgg
      301 caaaaccgaa gctgagcatt gtgtgggcaa tgagtggtga accccagtag acgacgaaaa
      361 ggtcgacgct cttcatcttt gtcaggtcga aggtcctgtc gtaatggcgc acggtgtaat
      421 ccgtctctgt cctgtaatcg acattgcgga tgccgtggat ggtcaccctg tcgccatcga
      481 tcgcggccca ggggagaagg gcaacgtcag gctgccagtt ccttgtattc gacggcggga
      541 tcaggagcca ccagaccaga acggccgcga aggcggcaag aaagacaaac cgagcggccc
      601 gacggcgata ccccacggtg aggacggcga gacccccgat gacaaaaagt cctgcagcga
      661 tgagtctgaa agagggaggc agatcggagt agtagacggc gagcgttgcc caggccgtca
      721 ttatcagaaa tgcgaacctc ctgaagacat tgccgatgcg tctgaagaac cggccgggaa
      781 atgtgtttct ggaacgcatg ctcatgggaa tgcaggaaac cgcccgcgca tggccgcggg
      841 cggtccccga tgatcctgcg ttatctttgg tcccgcaagg tctacttctc caccaccatt
      901 gccacggcct cggtcactct caggtagacg tcgtctccct tcttgatgtt cttgaagttc
      961 ttgacggact tatcgacggt gaaggtccgc acgtttcctt cggggccctt gagagaaatg
     1021 gtacgtttct tataatctat cttttcgacc ttcgccgtta cctccagcgt gtcaactacc
     1081 acagctccgg gcttcgcgcc cttcggcgcc acttccacgg tcccgaactc cgtggcggaa
     1141 ggcttctcgc ccgtcttcgc cacaaagaca gcgatcgatt catagaaggt cgtcttgacc
     1201 ttgtcgccgg cctttatctg gtcgaagttc ttcacttccg ggccgacctt gaaggcacgc
     1261 gccgtgccgt cgggcatctt gaaagtgacc gttctcttcg ccgcatccac ggcatcgata
     1321 acagccgtca tctcgatgac gtccaccata gccccggcgg gcttggggcc ggccttcttc
     1381 gccgctttct tctcctccga aaacccgacg ctcgcgatga agaccacgag caacgacgcc
     1441 agtaccactg acagtgttct tttcatatac atcctcctct gggatatttc aggccgcccg
     1501 gcggcctgtg tatttcaggt gatcacgaat ccgaacacct caccgcgaac acgcgtgggg
     1561 cggagaggca ctgctatcta ccacatgaaa gccgcgcggt acacagggat atctgtcgcg
     1621 catcgatcat caggccagcg cgttcctgac tgtccggcgg gcaccggcaa ggatggagtc
     1681 gaggtgggcc tcgtcgacaa agctctcggc gtacatcttg atgatgtcct ccgtgcccga
     1741 gggtcgcacc gcgaaccagc ccctcgccgt cgttaccttg acaccaccta tggccgcgtt
     1801 gttgcccggg gctttcgtca tcttcgacgt tatcgggtcc cccgccagtt cctcgtcctc
     1861 tatggcctcc ggtttcaggg acgacaccgc cgccctctcg gcagccgttg ccggggcgtc
     1921 gacgcgaacg tacagaggcc ttcccagcct gtcggccaac tcgccataga gttccccggg
     1981 gtccttaccc atc
//
LOCUS       DAOPOA010000481         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_61487_length_1991_cov_3.483988, whole genome shotgun sequence.
ACCESSION   DAOPOA010000481 DAOPOA010000000
VERSION     DAOPOA010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61487_length_1991_cov_3.483988"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PKJ17_09475"
     CDS             <1..816
                     /locus_tag="PKJ17_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOD76246.1"
                     /translation="HIQEEAEEVKVLVLMKQVADTEARIIISSDKKSLEVENKYVVNF
                     FDEFAVEEAIRIKEALKDVEITVCAFAPQRAVEALRTTIAMGADRAFLIDSTKHETDD
                     PLIVSRVLAGFARKDGFDVILCGRQAMDDESANVGPMVAEFLGIPHVSQVTKLSITGD
                     GKAGVESEIEGGRRTSEVSLPALFTCQKGLNEPRVPLITGVMKAMKTQIDILDPASIE
                     APFGPIDRESSKITVLSYEAPAGRPSVRIMEGASPEEKAQNLVRALKDEAKVL"
     gene            832..1812
                     /locus_tag="PKJ17_09480"
     CDS             832..1812
                     /locus_tag="PKJ17_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOD76247.1"
                     /translation="MTRDVFVVVECKDSKPRKASFELLSIGREMASRFSSTLFAVVLG
                     SGLADAADAVARFAGKTILVDDAALAEYGWDTYGSVLETILKKYEPMVVLGTSSVTGK
                     DLFPRMAARLSAAMVSDAVGVDMAGEGIRIKKPLYGGKVISWLAPKADSTVIVTFRPN
                     SFAVADAGDAGEIIEEAAAVAGNSAMKTMSSERKTSGKVDLQEADFIICGGRGMKAAE
                     NFAMLDEIADLMGGRTGASRTVIDSKWRDYDDQIGKSGKTVSPKLYIGCGLSGALHHI
                     MGMDTSKVIVAINKDPNAPIFQYADYGIVDDVFAILPALKDELKKTREET"
BASE COUNT          446 a          533 c          658 g          354 t
ORIGIN      
        1 cacatacagg aggaggctga agaagtgaag gtcctagtat tgatgaaaca ggtggctgat
       61 acggaagcgc gcatcatcat aagctccgat aagaagagcc ttgaggtcga gaacaagtat
      121 gtcgtgaact tcttcgacga atttgccgtt gaggaggcga tacgtataaa agaggcactg
      181 aaggacgtgg agataacggt gtgcgccttc gcgccgcaga gggccgtcga agccctgagg
      241 acgacgatag ccatgggcgc ggacagggcc ttccttatcg acagcacgaa acacgagacc
      301 gatgaccccc ttatcgtttc gcgcgttctc gcgggattcg cccggaaaga tgggttcgat
      361 gtcatactct gtggacgcca ggcgatggac gatgagagcg ccaacgtggg tcccatggtg
      421 gcggagtttc tcggcatacc gcacgtgagc caggtaacga aactcagtat caccggtgac
      481 ggcaaagccg gggtcgaaag cgagatagag gggggcagaa ggacgtcgga ggtctcgctg
      541 ccggcccttt tcacctgtca gaaaggcctc aacgagccga gggtgcccct cattacaggc
      601 gtgatgaagg ccatgaagac acagatagat atcctcgatc ccgcgtccat cgaggcgccc
      661 ttcggtccca tagacaggga gtcgtcgaag atcacggtgc tgtcctacga ggcaccggcc
      721 ggccggcctt cggtccgcat catggaggga gcgtcaccgg aagagaaggc gcagaacctc
      781 gtcagggcgc tcaaggatga agcgaaggtc ctgtagagag gaggattgca catgaccagg
      841 gacgtatttg ttgttgtgga atgcaaagac tcgaagccga gaaaggcatc gttcgaactg
      901 ctgtccatcg gcagggagat ggcctcgcgg ttctcgtcca cgctgttcgc ggtggtgttg
      961 gggtccggcc ttgcggacgc cgccgatgcc gtcgcccggt ttgcgggtaa gacaattctt
     1021 gtcgacgatg ctgcgctcgc ggagtatggg tgggatacct atggatcggt cctcgagact
     1081 atcctgaaga agtacgagcc catggtcgtt ctgggaacgt cgtcggtgac gggcaaggac
     1141 ctctttccca ggatggcagc gcgactttcc gccgcgatgg tgtccgacgc ggtcggtgtc
     1201 gacatggcgg gggaagggat aaggataaag aaacccctgt acggcggcaa ggtgatctcc
     1261 tggctcgcgc caaaggcgga ctcgacggtc atcgtcacct tcagacccaa ttcctttgcc
     1321 gtggccgacg cgggcgacgc gggcgagatc atcgaggaag cggccgcggt ggccgggaat
     1381 tcggcgatga agacgatgtc gtccgaaaga aagacctccg gcaaggtcga tctccaggag
     1441 gccgacttca tcatttgcgg aggaaggggc atgaaggccg ccgagaactt tgccatgctc
     1501 gatgaaatag ccgacctcat gggcggccgc acgggagcgt cgcggacggt catcgacagc
     1561 aaatggagag actacgacga ccagatcgga aagagcggga agacggtctc tccgaaactg
     1621 tacatcggat gcggcctgtc aggcgccctc caccacatca tgggcatgga cacctcaaag
     1681 gtcatcgtgg ccatcaacaa ggaccccaat gcccccatat tccagtacgc cgattacggc
     1741 atcgtggacg atgtcttcgc catccttccc gccctgaagg atgaactgaa gaagacacgg
     1801 gaagagacgt aaggggcatg gacaagatcg atgtcgtgat cgtcggggcc ggcctcgccg
     1861 gcctgtcctg cgcctgggtg ctttcggggc acggtctgca ggtcatcgtc gtggagcgcg
     1921 gcgacttcgc gggcagcaag aacgtgacgg gcggcaggct ctacctcaaa cctgtaagcg
     1981 gcatggcatc g
//
LOCUS       DAOPOA010000482         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_61664_length_1987_cov_2.170807, whole genome shotgun sequence.
ACCESSION   DAOPOA010000482 DAOPOA010000000
VERSION     DAOPOA010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61664_length_1987_cov_2.170807"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKJ17_09485"
     CDS             complement(<1..331)
                     /locus_tag="PKJ17_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76248.1"
                     /translation="MKKKFATGILVLLVAVMGAPSLWAQQQGNVVEKSLEDAFLGANP
                     EKTNTPAASKADEGRAAVSTATPAKPVLSDGQRLERLMGVTASKCIKGLETLKGPARA
                     FVVAAYST"
     gene            complement(328..915)
                     /locus_tag="PKJ17_09490"
     CDS             complement(328..915)
                     /locus_tag="PKJ17_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76249.1"
                     /translation="MAATRDDMRKLISGNGAPFSKYLVKVAGKGYLVIPGTVDQGNIS
                     IRPQGDNLLVTLPFDLAKEAGLVTNADIVPVAGGWCFGGKVAVMPGLYSPVVCTPTWL
                     PAGVSVSVDSNGKLEFGLAKKFKSETERLVSYMEMTKLLEGASGEVMGYFSTVPQLKK
                     LLDRMGQDARSDPGLSVLNATGADPFALVIVRRGR"
     gene            complement(1085..>1987)
                     /locus_tag="PKJ17_09495"
     CDS             complement(1085..>1987)
                     /locus_tag="PKJ17_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76250.1"
                     /translation="APAKDKKDLSPEDILKDTPPPQRTRGGYDPMKPQSVDKDGKLLS
                     GQTHMPDPDKQGTREQMWAYLYSRRSNGGFIAKADEKGKTSEGKGTSSTQPGGPETVQ
                     TVSGTTASSASGGHAQLPVQVQYHKPYKAVVDRTFTSGAKNTTFVATLSDPPLRGWKV
                     TGRATANYNDLRFDVDVNSVISPENKPYSVKGFVASIDQSDGIVSAVKHEDVAGSMLS
                     GIAKGASAFLDAFRSDTTTIEVASGGVTVTGQNKVADRGKEASLAAGSAIFDDMSKKM
                     TGSAKKTQTVIVERGVPVLVYFVP"
BASE COUNT          375 a          628 c          498 g          486 t
ORIGIN      
        1 ccgtgctgta ggcggcaacg acgaatgccc tggcagggcc tttcaacgtt tccaagccct
       61 ttatgcactt tgacgccgtc acacccatga gcctttccag tctctggcca tcgctcagta
      121 ccggcttcgc cggcgtggcg gtggatacgg ccgccctgcc ctcatccgcc ttcgaagcag
      181 ccggcgtatt tgttttctcc gggttcgcgc ccagaaaggc gtcttccagg gatttttcaa
      241 ccacgtttcc ctgctgctga gcccacaaag agggggcgcc catgacagcc acgagaagga
      301 caaggatgcc ggtcgcaaat ttcttcttca tcgccctctc cttacgatca ccagcgcgaa
      361 aggatcagct cctgtcgcat tcaataccga aaggccagga tccgaacgtg cgtcctgccc
      421 catacggtca agcagtttct tgagctgcgg aaccgtcgag aagtatccca tcacctctcc
      481 ggatgctccc tcgaggagct tcgtcatctc catataactc acaagcctct cagtctcgct
      541 tttgaacttc ttggcaagac cgaattccaa tttgccgttg ctgtcgaccg acacggacac
      601 gcccgccgga agccaggtgg gggtgcacac gaccggcgaa tacagcccgg gcatcacggc
      661 aaccttgccg ccgaaacacc atcctcctgc caccgggacg atgtcggcgt tggtcacgag
      721 acccgcttct ttcgccaggt cgaagggaag cgtcacgagc aagttatcac cctgaggtct
      781 gatggatata ttgccctggt caacagtgcc cggtatcacc agatagccct ttcccgcaac
      841 cttgaccagg tattttgaga aaggtgcgcc gtttcctgaa ataagcttcc tcatatcatc
      901 tctcgtcgcc gccatcgtat ccggtgtgat cgcatcctgt tccgtggatt ccgatatccc
      961 cacgttcagg aacgacattg tcttgcatcc ggtgaaagat gcggcgatca ctgctaacat
     1021 cagcaggaac cccagaccta tctttctttg tttcatcccc tttatcctcc cgcggtaaac
     1081 atgcctacgg cacaaaatac acgaggacag gcaccccccg ctccacgata acggtctggg
     1141 ttttctttgc actgcccgtc attttcttgc tcatgtcgtc gaatatggca gagccagctg
     1201 ccaggctggc ttccttcccc ctgtctgcga ccttgttctg ccccgtgacc gtcactccac
     1261 ccgatgctac ctcgattgtg gtcgtgtcac tccgaaacgc atccaggaag gcactggcgc
     1321 ccttcgcgat gcctgagagc atagacccag ccacgtcctc gtgttttacc gccgacacga
     1381 tcccgtcgct ctggtctatc gatgcgacaa agccttttac ggaatacggt ttattctccg
     1441 gtgatattac actgttgaca tccacgtcga acctgaggtc gttgtaattt gccgtcgcac
     1501 gccccgtgac cttccaaccg cgcaatggcg ggtcgctcag tgtagcgacg aatgtcgtgt
     1561 tcttagcccc ggaagtgaac gttctgtcaa ccacggcttt atacggttta tgatactgga
     1621 cctgcaccgg cagctgtgcg tgccccccgg aggctgaact ggcggttgtt ccggaaaccg
     1681 tctgaaccgt ctccgggcca ccaggctgtg tggatgacgt tcccttgcct tccgaagtct
     1741 tccctttctc gtccgctttt gcgatgaaac ctccgttact tctcctggag taaagatatg
     1801 cccacatctg ttctctcgta ccctgtttgt cagggtccgg catgtgggtc tgtcccgaaa
     1861 gcaatttgcc gtctttgtcg actgattgag gtttcattgg gtcgtatcca ccgcgagtcc
     1921 gctgcggggg aggcgtatct ttcaggatgt cttcagggga gagatccttc ttgtccttcg
     1981 cgggggc
//
LOCUS       DAOPOA010000483         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_61798_length_1984_cov_2.585277, whole genome shotgun sequence.
ACCESSION   DAOPOA010000483 DAOPOA010000000
VERSION     DAOPOA010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61798_length_1984_cov_2.585277"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>441
                     /locus_tag="PKJ17_09500"
     CDS             <1..>441
                     /locus_tag="PKJ17_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOD76251.1"
                     /translation="VINGLVAFIENRDIPLSELMEIIPGPDFPTQGIIYGRQGIVEAY
                     QSGRGHIVLRAKADIEKRKKDGREAIVITEIPYQVNKARLIENIVELINAKRIEGISN
                     IKDESSREGMRIVIDLKKGEIPEPILNKLYKHTQLQTTFGIIFLT"
     assembly_gap    442..451
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <452..1919
                     /locus_tag="PKJ17_09505"
     CDS             <452..1919
                     /locus_tag="PKJ17_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOD76252.1"
                     /translation="IVNNEPKLLNLKEMLHEFVEFRKEIVTKRSTFELNKALDRLHLL
                     EGLKTALDHIDEVIAIIKGSANTPEAREALIARFGFSEKQATNILEMRLQRLTSLERT
                     KILDDHKNTSDEIDRLRRVLADESLLIDIVKTELIEMRDKYGDKRLTEIADYVPEITL
                     EDMIKEEEVVVTITNKGYIKRTSLDQYRNQMRGGKGRTGIVVREEDFVDHLYIASTHS
                     NILFFTNIGKVHAVRVHTIPEASLTSKGKPVVHLINLEKDERITAVAHVKDYAPDRFL
                     FFTTKKGHVKRLSLDLLKNIRSTGIKAITLPDDDSLISVLETAGDGEIMIGTKFGLAI
                     RFNETEIRPMGRSAYGVRGIRFKKASDEVVSVEQVNESCTIFTVTQKGYGKCAPCSDY
                     RLQARGGSGIINVRCGIKNGSVVSLNRLSEQEDVILVTDTGRTIRFRSSNIPVQKRGG
                     LGVKLMGLRDGEVISGVAIVEAADDIVEEGMNPTDGNE"
BASE COUNT          512 a          596 c          554 g          312 t
ORIGIN      
        1 ggtcatcaac gggcttgtcg ccttcatcga gaacagggac atcccgctgt ccgagctcat
       61 ggagataatc ccgggacccg acttcccgac gcagggcatc atctacggaa ggcaggggat
      121 agtcgaggcc taccagtccg gcaggggaca catagtcctg cgcgccaagg cagacatcga
      181 aaaaagaaag aaggacggca gagaggccat cgtcatcacc gagataccct accaggtgaa
      241 caaggcccgc ctgatcgaaa acatcgtcga gctcatcaac gcgaagagga tcgagggcat
      301 atcgaacatc aaggacgaat cgagcaggga gggaatgcgc atagtcatcg acctcaagaa
      361 gggggagata cccgaaccca tcctcaacaa gctctacaag cacacccagc tccagacgac
      421 cttcggcatc atcttcctca cnnnnnnnnn ncatcgtcaa caacgaacca aaactgctga
      481 acctgaagga aatgctccac gagttcgtcg agttccgcaa ggagatcgtc acgaagaggt
      541 cgaccttcga gctcaacaag gctctggaca ggctccatct cctcgaaggg ctcaagaccg
      601 ccctcgatca catcgacgag gtgatcgcca tcatcaaggg ttccgccaac acacccgagg
      661 cccgcgaggc cctcatcgcc cgttttggct tttccgagaa acaggcgaca aacatcctcg
      721 agatgcggct ccagcgattg acctccctgg aaaggacgaa gatactcgac gatcacaaga
      781 acacgtccga cgagatcgac cgtttaaggc gggtactggc cgacgagtct ctcctcatcg
      841 acatcgtgaa gacggaactt atcgagatgc gcgacaagta cggtgacaag cgcctgacgg
      901 agatcgccga ttacgtcccc gagataaccc tcgaggacat gatcaaggaa gaagaggtgg
      961 tcgtcacgat caccaacaag gggtacataa agaggacgtc cctcgaccag tacaggaacc
     1021 agatgcgtgg cggcaaagga agaacgggca tcgtggtccg ggaggaagac ttcgtcgacc
     1081 acctctacat cgcgtcgacc cactccaaca tactcttctt cacgaatatc ggcaaggtcc
     1141 acgcggtaag ggtccatacc atcccggaag cctccctcac gtcgaaggga aaacccgtcg
     1201 ttcaccttat caaccttgag aaagacgaac gcataacggc ggtcgcccac gtcaaggact
     1261 atgcgcctga ccggttcctc ttcttcacga cgaagaaagg ccacgtcaag cgactcagcc
     1321 tcgacctcct caagaacatc aggtccacgg gcataaaggc cataaccctg cccgacgacg
     1381 acagcctcat cagcgtcctc gagacggccg gggacgggga gatcatgatc ggcacgaaat
     1441 tcggcctcgc gatacgattc aacgagaccg agatacggcc catgggccgg tccgcctatg
     1501 gcgtccgcgg catccggttc aaaaaggcct ccgacgaggt tgtctctgtg gaacaggtca
     1561 atgaatcgtg cacgatcttt accgtgacgc aaaagggcta cggcaagtgc gccccctgct
     1621 ccgactaccg tctgcaggca cgcggcggct cggggatcat caacgtcagg tgcgggataa
     1681 agaacggcag cgtcgtcagt ctcaaccgcc tgagcgagca agaggacgtc atcctcgtca
     1741 ccgacaccgg gcgcaccatc aggttccgtt ccagcaacat acccgtgcaa aagcgcggag
     1801 gccttggtgt caagctcatg gggctgcgcg acggtgaggt cataagcggc gttgccatcg
     1861 tggaggcggc cgacgacatc gtcgaggagg gcatgaaccc aacggacggg aacgaatgaa
     1921 gataggtgtc atcggcgcgg gggcatgggg aacggccttt gccatccacc ttgcccgaac
     1981 gggg
//
LOCUS       DAOPOA010000484         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_61804_length_1984_cov_2.371695, whole genome shotgun sequence.
ACCESSION   DAOPOA010000484 DAOPOA010000000
VERSION     DAOPOA010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61804_length_1984_cov_2.371695"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKJ17_09510"
     CDS             <1..221
                     /locus_tag="PKJ17_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76253.1"
                     /translation="SPLWDGADARENLARHIEVLRAEGIANILFRIDLGHGWHARLYP
                     VIEANGFVPSIVIPYQGKADWLFFTLRD"
     gene            232..1356
                     /gene="hisC"
                     /locus_tag="PKJ17_09515"
     CDS             232..1356
                     /gene="hisC"
                     /locus_tag="PKJ17_09515"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOD76254.1"
                     /translation="MFDVPLNKLVPEYIKKFEAYIPSRPDPELMRLYKVDHLWRLNNN
                     ENPLGPPEGSARIIAEFPPPRASIYPSGDSWYLRVKLAEKFGLDPDQIVFGNGANEAI
                     AFVIKAFCQEGDNIITADKTFAVYEWVAEFSGFEARLVPLKDFGFDDEGMLGRIDDRT
                     KILFVCNPNNPTGTYWDETRLRRFLDKVAGRQIVVVDEAYCEFVEADDYPDAMKLLKE
                     YPNLVTFRTFSKMYGLAGLRIGYLAGSRDVVDVIRRTCIVYSVNGVAQEAALAAIGDD
                     EHVARTRRHVNAEKAYLLRELAGLGVETHAGEGCYVMVRLPLSDTLVYRQLMARGVMI
                     RTMTGFRFPNWIRVSVGVHEAMEDFIGGLGEVLTDREAVL"
     gene            1353..>1984
                     /locus_tag="PKJ17_09520"
     CDS             1353..>1984
                     /locus_tag="PKJ17_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD76255.1"
                     /translation="MKEKVRRFLEGPRLDGLSKVTGRERYAADEYAGEFLWAGVKRAL
                     VPHARLLAVHTTRAAAHPGVLRVLTFRDVGGSNRLGIVRNHQPVLVDEVIRHAGDAVA
                     LVVAESREALREALDLITFDHEPLPGVFDPEDALEEGAPRIHEDNEEGNLMRAVRVKR
                     GDPDVAFRGCDVVVEGMLETPRQEHAYLETEAGWAYVDDDGVLVVVTSTQT"
BASE COUNT          367 a          589 c          657 g          371 t
ORIGIN      
        1 tgtcgcccct gtgggacggc gccgatgccc gggagaacct ggcaagacac atcgaggtct
       61 tgagggccga agggatagcg aatatccttt tcaggatcga cctcggccac gggtggcacg
      121 cgcggctcta tcccgtcatc gaggcgaacg ggttcgtgcc gtccatcgtg atcccctacc
      181 agggcaaggc ggactggctc tttttcaccc tgcgggattg aggtctttcg cgtgttcgac
      241 gtccccctca acaagcttgt tcccgaatac atcaagaagt tcgaggcgta catcccgtcc
      301 cgtcccgacc ccgagctcat gagactttac aaggtggacc acctgtggcg gctcaacaac
      361 aacgagaacc ccctgggccc tcccgagggc tcggctcgga tcatcgctga gtttccgccg
      421 ccgagggcga gcatctatcc cagcggcgat tcctggtacc tgcgcgtgaa gctggccgag
      481 aagttcggtc tcgaccccga ccagatcgtg ttcggcaacg gggcgaacga ggccatcgcc
      541 tttgtcatca aggccttctg ccaggaaggg gacaacatca taacggccga caagaccttt
      601 gccgtttatg aatgggtggc ggagttttcc ggtttcgagg cgaggctcgt tccgctcaag
      661 gatttcggtt ttgacgacga ggggatgctt ggccgcatag acgacaggac gaagatactc
      721 ttcgtgtgca atccaaacaa ccccacaggg acctactggg acgagacgag actgagaagg
      781 ttcctcgaca aggtcgccgg aagacagatc gtcgtcgtcg acgaggcgta ctgcgaattc
      841 gtcgaggccg atgactatcc cgacgcgatg aagctcctca aggagtaccc caacctcgtg
      901 accttccgga ccttttccaa gatgtacggt ctcgcggggc tccgcatcgg ctacctcgcg
      961 gggagccgtg acgtggtgga cgtgatccgg cggacctgta tcgtctattc ggtcaatggc
     1021 gttgcccagg aggcggctct cgcggccatc ggtgacgacg agcacgtcgc ccggacgagg
     1081 cggcacgtca atgccgagaa ggcctacctc ctgagggagc tcgcgggtct cggagtggag
     1141 acgcacgccg gtgaagggtg ctacgttatg gtgaggctcc ctctgagcga tacccttgtc
     1201 taccggcagt tgatggcgag aggggtgatg atacggacga tgacgggttt tcgttttccc
     1261 aactggatcc gcgtgagcgt cggggtccat gaggcgatgg aggacttcat cggcggtctc
     1321 ggtgaggtcc ttaccgatag ggaggctgtc ctgtgaagga gaaagtgaga cgtttcctcg
     1381 agggaccacg cctcgacggt ttgtcgaagg tcactggacg ggagaggtac gccgccgacg
     1441 agtacgcggg agagttcctg tgggccggcg tgaaaagggc cctcgtaccc cacgcccggc
     1501 tccttgccgt tcacaccacg cgcgccgcgg cgcatccggg tgttcttcgc gttctgacct
     1561 tccgggatgt cggcgggtcg aacaggctgg ggatcgtcag gaaccatcag ccggtgctcg
     1621 tcgatgaggt catccgccac gccggggacg ccgtcgccct ggtggtcgcc gaaagccgcg
     1681 aggccctccg cgaggccctc gacctcatca ccttcgacca tgagccgctg ccgggcgtct
     1741 tcgaccctga agatgccctg gaggaaggcg cccccaggat tcacgaggac aacgaggaag
     1801 gcaacctcat gagggccgtt cgcgtgaagc ggggagaccc cgacgtggcc tttcgcggct
     1861 gcgatgttgt ggtcgagggc atgctcgaga cgccacgcca ggagcacgcg tacctggaga
     1921 cggaggccgg gtgggcctat gtggatgacg acggggtgct cgtcgttgtg acgtcgacgc
     1981 agac
//
LOCUS       DAOPOA010000485         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_61966_length_1980_cov_2.724675, whole genome shotgun sequence.
ACCESSION   DAOPOA010000485 DAOPOA010000000
VERSION     DAOPOA010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61966_length_1980_cov_2.724675"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /gene="flgA"
                     /locus_tag="PKJ17_09525"
     CDS             <1..792
                     /gene="flgA"
                     /locus_tag="PKJ17_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOD76256.1"
                     /translation="SDDQQPEEIAVDTPINTNRDGRMFRGPEAVRSLCVGLLILFFFI
                     AAAVGSLRTVHAAGESAAEARIIKFVKEIYPGGDAVRVRLTTIPAQLREKVKIVNLSF
                     VRIPDVSGDGVCSVEIETAPGRLRTVQVPFRVFTKRELYVLKEAGQKGDTIDPKDVVV
                     RETYMNGKGLGYPASIEDVVGKVLKRDVPANTLVTYQILEDRVVVRRGDTVTIIAETD
                     KLVVRAKGKTVDKGRVGDVIRVKNIASGKEVMAKVVNSSSVKVEF"
     gene            805..1506
                     /locus_tag="PKJ17_09530"
     CDS             805..1506
                     /locus_tag="PKJ17_09530"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOD76257.1"
                     /translation="MKTSAVLLMVILGAVLLAGCQSTSQSVIKDEPFVIDNTYKNAKP
                     ALQPPAGTIYRGVNANSNFLGDHRARGVGDIVTVKIYEVTNASEKAGTKTAKSATTTA
                     GIPNFLGLESNFYPSSITPDKMINANTKNDFDGSGETTRGGSLTATITARVVDVLPNG
                     NLAIEGKREIVINNEKKEILVQGIVRPRDLDYDNSVYSTQVADAKIIYTGVGVLGEKQ
                     RPGWLARIMDTVWPF"
     gene            1518..>1980
                     /locus_tag="PKJ17_09535"
     CDS             1518..>1980
                     /locus_tag="PKJ17_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOD76258.1"
                     /translation="MSYRERVHRSTTIVCVLFFIAIFLLFMVGMADAARIKEMGYING
                     ARTNQLIGYGLVIGLAGTGDKSNTIFTNQSLANMLETMGIRVDPKTTKVNNVAAVMVT
                     CNLPPFARIGSKVDVTVSSIGDAKSIEGGVLVTTPLKGVDGQVYAMAQGSTV"
BASE COUNT          457 a          546 c          622 g          355 t
ORIGIN      
        1 tcagatgacc aacagcctga ggagatagcg gtggataccc cgataaacac gaaccgtgat
       61 ggaaggatgt tccgcgggcc ggaggcggta agaagtttgt gcgtgggtct tctgatactg
      121 ttcttcttca tcgccgccgc ggtcgggtcc ttaaggacgg tccatgccgc cggagaatcc
      181 gcggcggaag ccaggataat caagttcgtc aaggagatct atcccggagg cgacgccgtt
      241 cgggtcagac tgacgacgat tcccgcccag ctgagggaaa aggtgaagat cgtcaacctc
      301 agctttgtgc gcatacctga cgtcagcggc gatggagtct gctctgtcga gatcgagacc
      361 gctccgggca ggctgcgcac cgtgcaggta ccgttcaggg tctttacgaa gcgggaactc
      421 tacgtgctga aagaggcagg acagaagggt gataccatcg atccgaagga cgtggtggtc
      481 cgcgagacct acatgaatgg caaaggcctt ggctacccgg cgtctatcga agatgttgtc
      541 ggcaaggtcc tcaagaggga cgtgccggcc aatacgcttg tcacctacca gatcctcgag
      601 gaccgcgtcg tcgtcaggcg gggtgacacg gtgaccataa tcgccgagac cgacaagctc
      661 gtcgtgcgcg cgaagggcaa gaccgtcgac aaggggcggg tgggcgacgt gattcgggtt
      721 aagaacattg cctccgggaa agaggtgatg gcgaaggtcg taaacagcag ctcggtcaaa
      781 gtcgagttct aggagtgtga gtttatgaag acgagcgccg tattgttgat ggtcatcctg
      841 ggggcggtcc ttctggcagg ttgccagagc accagtcaat cggtgataaa ggatgagccc
      901 ttcgtcatag acaatacata caagaacgcg aaaccggctt tgcagccccc cgcgggcacc
      961 atttaccgcg gagtcaacgc gaacagcaac ttcctgggcg atcacagggc ccggggagtg
     1021 ggtgacatcg taaccgtcaa gatctatgag gtgacgaacg catcggagaa agcgggcacg
     1081 aagacggcaa agtcggccac gacaacggcg ggcatcccca atttcctggg gctcgagtcc
     1141 aacttctatc cctcgagcat cacccccgac aagatgataa acgccaacac caagaacgac
     1201 tttgacggct ccggcgagac cacccggggc ggctctctga ccgcgaccat cacggcgcgc
     1261 gtcgtcgacg tgcttcccaa cggcaacctg gccatcgaag ggaagaggga gatcgtcatc
     1321 aataacgaaa agaaggagat cctggtccag ggcatcgtga ggcccaggga cctcgattat
     1381 gacaattccg tgtactcgac ccaggtcgcg gacgccaaga tcatctacac cggcgtggga
     1441 gtactgggcg aaaagcagag gccaggctgg ctggcacgca tcatggacac cgtatggcca
     1501 ttctgacggg agacggcatg agttaccggg aacgtgtcca tcgcagcacg acgatagtct
     1561 gtgtcctctt cttcatcgcc atcttccttc tcttcatggt tgggatggcg gacgcggccc
     1621 gcatcaagga gatgggctac atcaacggag cgaggaccaa ccagcttatc ggctacgggc
     1681 tcgtcatcgg tcttgccggc acgggggaca agtcgaacac gatcttcacg aaccagtccc
     1741 ttgccaatat gctggagacc atgggcatca gggttgaccc caaaacgacg aaggtgaaca
     1801 acgttgccgc cgttatggtc acctgcaacc tgcctccctt cgcgcggata gggagcaagg
     1861 tcgacgtcac cgtctcttcc atcggcgacg ccaagagcat tgaaggaggc gtcctggtga
     1921 cgacgcccct caagggggtc gatgggcagg tgtatgccat ggcgcagggt tcgacggtgg
//
LOCUS       DAOPOA010000486         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_62052_length_1978_cov_3.805512, whole genome shotgun sequence.
ACCESSION   DAOPOA010000486 DAOPOA010000000
VERSION     DAOPOA010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62052_length_1978_cov_3.805512"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PKJ17_09540"
     CDS             <1..955
                     /locus_tag="PKJ17_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOD76259.1"
                     /translation="NFFTVIIVGLIILVVIAFLKTIRVVPQKVAFIVERLGKYNATLD
                     AGLHVLIPFIDKVSYRHTLKEQAIDVPSQTCITRDNIAVEVDGILYLQVIDPQKASYG
                     INNYQFAATQLAQTTMRSVIGKLELDRTFEERDTINTTIVEAVDKASDPWGVKVTRYE
                     VKNIVPPQSIKDAMEKQMRAEREKRAMIAESEGDKQAKINRAEGVKQEMIATSEGEKQ
                     KRINEAEGRAAEIERVAKATANGLREIALAINEQGGINAVNLRIAEQYINEFGKLART
                     NNTLILPANLGDLAGLVSTVTTILKDQTKDTGVRAQGAEKR"
     gene            1131..1568
                     /locus_tag="PKJ17_09545"
     CDS             1131..1568
                     /locus_tag="PKJ17_09545"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HOD76260.1"
                     /translation="MKVSILAALVIVLALVWCGGVQAAEPNMKDGLWEIKTKVEMKGM
                     PASIPPSVTKQCLTKKDSVPGQGKEKNPNCKITDQKVSGNTVTWAMVCKEKDGTVESK
                     GSITYKGDTFDGTTTTLIKNKGMQAQQVSTKMSGKRLGPCDKK"
     gene            1592..>1978
                     /locus_tag="PKJ17_09550"
     CDS             1592..>1978
                     /locus_tag="PKJ17_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76261.1"
                     /translation="MKIKIRKSRKTTINEVDFDNLEFGVHLSDHMYYVDYADGHWSEP
                     RIEPYGPMKMYPALSTLHYGQAVFEGLKAFLGVDGKIRLFRPDKNHERYNVSCERLCI
                     PTLSWEMFFEGMRKLVAIDRAWIPVKR"
BASE COUNT          533 a          559 c          569 g          317 t
ORIGIN      
        1 gaacttcttt actgtcatca tcgtcggtct catcatactc gtggtcatcg cctttctgaa
       61 gaccatacgc gttgtccccc agaaggtcgc cttcatcgtg gagcgcctcg gcaagtacaa
      121 cgcgacgctg gacgccggcc tccacgtcct catacccttt atcgacaagg tctcctacag
      181 acacaccctg aaggaacagg ccatcgacgt cccctcccag acatgcatca cgagggacaa
      241 catcgccgtc gaggtcgacg gcatcctcta ccttcaggtc atagatcccc agaaggcgtc
      301 ttacggcatc aacaattacc agttcgcggc cactcagctc gcccagacga ccatgaggag
      361 cgtcatcggc aaactggagc tcgacaggac cttcgaggag cgggacacga tcaacaccac
      421 catcgtcgag gccgtcgaca aggcgtccga tccctggggc gtgaaggtca cccggtacga
      481 ggtcaagaac atcgttccgc cgcagagcat caaggacgcc atggagaaac agatgcgcgc
      541 ggagcgtgag aagagggcca tgatcgctga atccgaggga gacaagcagg ccaagatcaa
      601 ccgcgccgag ggtgtcaagc aggagatgat cgccacctcc gaaggcgaga agcagaagag
      661 gatcaatgag gccgaaggcc gggcggcgga gatagagcgc gtggcaaagg caaccgcgaa
      721 cggcttgagg gagatcgcgc tcgctatcaa cgagcagggc ggcatcaacg ccgtcaacct
      781 tcgcatcgcc gagcagtaca taaacgagtt cggcaagctg gcgagaacga acaacaccct
      841 catcctcccc gccaaccttg gcgacctggc aggcctcgta tcgacggtaa cgacaatact
      901 gaaggaccag acaaaggata caggggtcag ggcccagggg gcggagaaaa gatagacgat
      961 agcggggcag catacacgac gatcgccctg gacaagaccg tcggaaccgc acatttttct
     1021 gactctcaag cggttcaagc gactcaagcc gtctttctct tgacccatcg gccccattag
     1081 tgataccttt ttttaccgaa gcatttgtca ggaaaagatc aggaggacat atgaaagtga
     1141 gcattcttgc agcactcgtc atcgttctgg ctctcgtgtg gtgcggcggc gtgcaggccg
     1201 cggaaccgaa catgaaggat ggtctctggg agatcaagac gaaggtggaa atgaagggca
     1261 tgcccgcgag catccccccg tccgtaacga agcaatgcct cacgaagaag gacagcgtgc
     1321 ccgggcaggg caaggagaaa aacccgaact gcaagatcac cgaccagaaa gtgagcggca
     1381 acacggtgac gtgggccatg gtctgcaagg aaaaggacgg caccgtggaa agcaagggaa
     1441 gcatcacata caagggcgac acctttgacg ggacaacaac gacgctcatc aagaacaaag
     1501 gcatgcaggc ccagcaggtt tcgacaaaaa tgagcggcaa acgcctcgga ccctgcgaca
     1561 agaaatagga aagaacgcga ggaagagagc tgtgaagatc aagataagga agagcagaaa
     1621 aacaacgatc aacgaagtgg acttcgacaa cctggagttc ggcgtccatc tctccgatca
     1681 catgtactac gtggactacg cggatggaca ctggagcgag ccacgcatcg agccttatgg
     1741 cccgatgaag atgtacccgg ccctttccac gctgcattac ggccaggcgg tctttgaagg
     1801 cctcaaggcc tttctcggag tggacggaaa gatccgactt tttcgcccgg acaagaacca
     1861 tgagagatac aacgtatcct gtgaacgcct gtgcatcccc actctctcgt gggaaatgtt
     1921 cttcgagggg atgagaaagc tcgtggcgat cgacagggca tggatccccg tcaagcgc
//
LOCUS       DAOPOA010000487         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_62075_length_1978_cov_2.124805, whole genome shotgun sequence.
ACCESSION   DAOPOA010000487 DAOPOA010000000
VERSION     DAOPOA010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62075_length_1978_cov_2.124805"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKJ17_09555"
     CDS             <1..489
                     /locus_tag="PKJ17_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76262.1"
                     /translation="IAGCMSVEGDDGYKHATQNDIEGLRSIFNAKTLFAPGCEINPLV
                     AAKDTNGLLGSLFAGKKLADINLQNRWHYAADPAIDKNTVALKDLRGGDDKQIKDCLC
                     RCLEPKGGRFHCRYDLHNRGNSPSCRDLANGPCLCQAAGCFRRKPPVSGECYDNCMKR
                     YR"
     gene            complement(504..1142)
                     /locus_tag="PKJ17_09560"
     CDS             complement(504..1142)
                     /locus_tag="PKJ17_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76263.1"
                     /translation="MKTWNEKTAEFDLAGKPLTKALLDKAAVDLGTMGEACVLALGPC
                     EGLDEMLSRAVSADISALLLPSLMDRLNASGESLPFPRDRVLTFVCGIWFLTAVVPRL
                     NDEGIPVDMHDFARELGRFFFMPYDEENREGLVKIGIQYWKELRAEPPVSVIEWDQAF
                     SQMVFIHYEQMTNRTIDLGDFDLAGSIGKMLTVFLSSAFTLPSPPDDGPDTL"
     gene            complement(1231..>1978)
                     /locus_tag="PKJ17_09565"
     CDS             complement(1231..>1978)
                     /locus_tag="PKJ17_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76264.1"
                     /translation="MNPADFGKAFQGRQVSMQNPPSARPGRAYADWPNRKDSKQVPAD
                     SSLAGNHFVHGNYLPDIRFAIDRGHDFMAPVEFTSTKGSPEDSFQFQWNAIPTATGYF
                     ATAMGSDEKTGTMIIWSSSDVREAGYSLMNFLSDEDVRRLVRDKVVMAPSTTQCAVPK
                     GIFKDAQGAMIQFIAYGDELNISYPPRPKDPIWAVKVRRKSTSMLPLMAMEGRGGPGR
                     APRDEGAPPPEKKEDEGITPGKLLKGIFKW"
BASE COUNT          409 a          587 c          568 g          414 t
ORIGIN      
        1 atagcgggct gcatgtctgt tgaaggcgat gacggataca aacacgccac tcagaacgac
       61 atagagggac ttcggtctat cttcaacgcg aaaacgctct ttgcgcccgg ctgtgaaatt
      121 aaccccctcg ttgccgcaaa ggacacaaat ggacttctag gctccctatt tgcgggcaag
      181 aaactcgccg acatcaatct ccagaatcgc tggcactatg cggccgatcc ggcgatcgat
      241 aagaacacag tagcgctcaa agacctcagg ggcggcgacg acaaacagat caaggattgt
      301 ctgtgcaggt gccttgaacc gaagggggga aggtttcact gccgctacga tcttcacaac
      361 agggggaatt ctccttcctg ccgcgatctt gccaacggac cctgtctctg ccaggccgcc
      421 ggatgtttca gaagaaagcc gcccgtaagc ggtgaatgtt acgataattg catgaaacgg
      481 tacaggtagc cggcgggcgc gccttacaag gtgtctggac cgtcgtccgg aggggatggg
      541 agggtgaagg cggaggagag gaagacggtc agcatcttgc cgatgctccc ggcaaggtcg
      601 aagtccccaa ggtcgatggt acggttcgtc atctgttcgt agtggatgaa gaccatctgg
      661 gagaacgcct ggtcccactc gatgaccgag accggcggct cagcccgcag ttccttccag
      721 tactggatgc cgatcttgac gaggccttcc ctgttctctt cgtcgtaggg catgaagaag
      781 aaccttccca attcccgcgc aaagtcgtgc atgtccacgg ggatcccttc atcgttgagc
      841 cgggggacca ctgccgtcag gaaccagata ccgcaaacga aggtgaggac cctgtccctg
      901 ggaaagggaa gactctcgcc cgaggcgttg agcctgtcca tgagcgaagg aaggagaagc
      961 gcggagatat cagccgaaac cgccctgctc aacatctcat caaggccctc gcagggcccc
     1021 agggcaagaa cacaggcttc ccccatcgtg ccgaggtcca ccgccgcctt atcgaggagc
     1081 gccttcgtca gcggcttgcc cgcgagatcg aattcagctg ttttttcatt ccaggttttc
     1141 atacgcgtac cgcgcgggtg atgggtaata ggttataggc tatgggaaca cctctccccc
     1201 ctattaccta tcacccatca cctctcttct ttaccactta aatatcccct tcagcaactt
     1261 gccgggcgtt atcccctcat cctccttctt ttcgggggga ggagcacctt cgtcacgggg
     1321 tgccctgccg ggtcctccgc ggccttccat tgccataagg ggcagcatcg acgttgattt
     1381 gcgtcttacc ttcacggccc agatggggtc cttcggtcgc ggcggatagg agatgttcag
     1441 ctcgtcgccg taggctatga actggatcat cgcgccctga gcgtccttga agatgccttt
     1501 cggtacggcg cactgggtcg tcgagggcgc catgacgacc ttgtccctca cgagacgccg
     1561 cacatcctcg tcggaaagga aattcatgag cgagtacccg gcctcccgga catcgctgga
     1621 gctccagatg atcatcgttc ctgtcttctc gtcgcttccc atggcggtcg caaaataccc
     1681 cgtcgccgtg gggatggcgt tccactggaa ctggaagctg tcctcggggc tgccttttgt
     1741 cgaggtgaat tccacgggag ccatgaaatc atgcccgcgg tctatggcaa agcgaatgtc
     1801 gggaagatag ttgccgtgga caaaatggtt gccggccagc gaactgtccg ccgggacctg
     1861 tttcgagtcc ttcctgttcg gccaatccgc gtacgcccgg cccggacggg ccgaaggcgg
     1921 gttctgcatc gatacctgcc gtccctgaaa ggcctttccg aaatcggctg gattcatc
//
LOCUS       DAOPOA010000488         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_62227_length_1974_cov_2.475769, whole genome shotgun sequence.
ACCESSION   DAOPOA010000488 DAOPOA010000000
VERSION     DAOPOA010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62227_length_1974_cov_2.475769"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKJ17_09570"
     CDS             complement(<1..726)
                     /locus_tag="PKJ17_09570"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOD76265.1"
                     /translation="MNWKRKDLLGIRDLSKEEMLFILDTAESFKDISKREIKKVPTLR
                     GKTVITLFYEPSTRTRTSFEIAAKRLSADTINISSSTSSSTKGESLKDTARNLESMRP
                     DAIVIRHGMPGAPHMLAGILDSSIINGGDGAHEHPTQALLDLFTMRDKKGRIDGLKVV
                     IVGDIAHSRVARSNIFSLKSFGNDVICCGPPTMIPPHIETLGVKVEYDLKRAVRKADV
                     IMMLRIQKERGGTTYIPSTKEYST"
     gene            complement(726..1256)
                     /gene="pyrR"
                     /locus_tag="PKJ17_09575"
     CDS             complement(726..1256)
                     /gene="pyrR"
                     /locus_tag="PKJ17_09575"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOD76266.1"
                     /translation="MKKRVILDKKAIGRTVTRITHEILEKNAGCTELCLIGIRTRGVY
                     LSQRVHAKVTDVEGITLPIGALDITMYRDDISKLRWPEVKKTEISFDVNNMTVVLVDD
                     VLHTGRTTRAAIDAIMDLGRPKRIQLAVLVDRGERELPIHPDYAGIVCPVNENEEVLV
                     RMSEIDGKGEVVIVKA"
     gene            complement(1257..1436)
                     /locus_tag="PKJ17_09580"
     CDS             complement(1257..1436)
                     /locus_tag="PKJ17_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD76267.1"
                     /translation="MRPYISRASCIDCRECVSICPYEVFTEKDGTVVVETPEDCVECG
                     ACVENCRQEAIYFDD"
     gene            complement(1742..>1974)
                     /locus_tag="PKJ17_09585"
     CDS             complement(1742..>1974)
                     /locus_tag="PKJ17_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOD76268.1"
                     /translation="QIVLKNTATHNGDLNTPFVMIENGAVFNGSCVMERRQQTFVPEP
                     SVETTAYAEPEPAAAEESSESSYGEENEEYES"
BASE COUNT          353 a          543 c          568 g          510 t
ORIGIN      
        1 ggtggagtat tcctttgtcg aaggtatgta cgtcgtcccg cccctctctt tctggatcct
       61 cagcatcatg atgacgtcgg ctttcctgac ggcgcgcttg agatcgtact cgaccttgac
      121 ccccagggtt tcgatgtggg gaggtatcat cgtgggcggt ccgcagcaga tgacgtcgtt
      181 cccgaaactc ttgagggaga atatgttcga ccgggcgacc ctgctgtggg ctatgtcccc
      241 gacgatgacc accttgaggc cgtcgatacg ccctttcttg tcgcgcatcg tgaaaaggtc
      301 gaggagggcc tgtgtagggt gttcgtgggc cccatcccct ccgtttatga tggaagagtc
      361 gaggatgccc gccagcatgt ggggcgcacc cggcatgccg tggcggatga cgatggcgtc
      421 ggggcgcatg gattccaggt tcctggcggt gtctttgagg ctttcgcctt tcgtcgaact
      481 gctggtgctg gaggatatgt ttattgtgtc ggcgcttaag cgcttcgcgg ctatttcgaa
      541 ggatgtgcgc gtccgggtgc tgggctcgta gaagagggtg atgaccgtct tgccccggag
      601 cgtggggacc tttttgatct ctcttttaga aatgtccttg aaggattcgg ccgtgtcgag
      661 aataaaaagc atctcctcct tagagaggtc gcgaatgccg aggaggtctt ttctcttcca
      721 gttcatcagg cttttacgat caccacttca cccttgccgt ctatttcgct catccggacg
      781 aggacctctt cgttctcatt cacgggacag acaattcccg cataatcggg gtgaatgggt
      841 aattccctct caccccggtc cacgagaacg gcaagctgta tcctcttcgg ccgtcccagg
      901 tccatgatgg cgtctatggc ggcccgcgtc gtccggcccg tgtgcaggac gtcgtcgacg
      961 agcacaacgg tcatgttgtt gacatcgaag gatatctcgg tcttcttgac ctcgggccac
     1021 ctgagcttcg agatatcgtc gcgatacatg gtgatgtcaa gggcgccgat cggcagtgtg
     1081 atgccttcca cgtccgtgac cttcgcgtgg acgcgctggg acagatagac gcccctcgtc
     1141 ctgatcccga tgagacacag ctccgtgcat cccgcgttct tctccaggat ctcgtgggtg
     1201 atccgggtga ccgtcctccc gatcgccttc ttgtccagaa tgaccctttt tttcatttaa
     1261 tcgtcaaagt agatggcttc ttgcctgcaa ttctcgacgc aggccccgca ctcgacgcaa
     1321 tcttccggtg tctcgaccac gacggtaccg tccttttcgg tgaatacctc gtatgggcat
     1381 atggacacac attccctgca atcgatgcag gacgcccggg aaatataagg tcgcacggta
     1441 ctttagtact atagaaagtt ttggaggaaa aagcaacaag aagacagggg gtgaagaagg
     1501 ttcaaggttt caggtgaaag acagttccgg gttccgggtt gcgagttaaa agccggagag
     1561 ggacagtctc cttatgagtc ctacgcgtcc tataggtccc atatgtccca taaattccta
     1621 taggtcctat ccgtcctcgc ggtgatcctg tccccttttg gatatcgccg agagccggta
     1681 gaggggccgt cctcgtgccg gctttcggct tttaagagag ttttcctgga gttgatcgtt
     1741 tttaggattc gtattcctcg ttctcctcgc catatgagga ttcgctgctt tcctccgccg
     1801 ccgcaggttc cggttccgcg tacgccgtgg tctccacgga aggttccggg acgaaggtct
     1861 gctgtcttct ctccatcacg caggagccgt tgaagaccgc cccgttctcg atcataacga
     1921 agggcgtgtt gaggtcgcca ttgtgcgttg ccgtgttctt gaggacgatc tgct
//
LOCUS       DAOPOA010000489         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_62258_length_1973_cov_3.253910, whole genome shotgun sequence.
ACCESSION   DAOPOA010000489 DAOPOA010000000
VERSION     DAOPOA010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62258_length_1973_cov_3.253910"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="PKJ17_09590"
     CDS             complement(<1..1114)
                     /locus_tag="PKJ17_09590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOD76269.1"
                     /translation="MSPEKRLIIIDDEVTLLAVLQEFLQQFGLEVFTYQSMPDLRAEL
                     GDKRPHAVLCDIVLPGISGIEILKEIKKIDRRIPVIMMTGYAEERERLESLSNGAYAL
                     LTKPFKSFEELYHIVNNSMDHYREVLRTEDLTAQVEERFRREKINILELEFLKNLQRM
                     IGETEDPVFVLRNAHTLLGSFLDFQFFGTMLVGGQEADIHIFPETGGDPELRRSMVSA
                     LAGWGNRVPGQISPGTASVVSELRAAKRLYGYAALYRQEPFNEEETSIFTRFCSHIAL
                     TLEKISLFEEIRTLSRHDGLTGVFNHACIVGELGAEIERAKRYDSPFSVILFDVDDFK
                     LINDRYGHLAGDQVLKGITRTMKDNLRNIDRLGRYGG"
     gene            complement(1175..1393)
                     /locus_tag="PKJ17_09595"
     CDS             complement(1175..1393)
                     /locus_tag="PKJ17_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOD76270.1"
                     /translation="MKEKVQQALEKIRPLLQRDGGDIQLIDVVDGIVKVKLQGACGSC
                     PMSMMTLKMGVEKQLKQEIPEVKEVVSV"
     gene            complement(1390..1893)
                     /locus_tag="PKJ17_09600"
     CDS             complement(1390..1893)
                     /locus_tag="PKJ17_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD76271.1"
                     /translation="MPLTPWKSMWETKFPSLREEMDRVFEEFFGEAGFPTLREADWLP
                     AVDVVERKGEIVVIMDIPAIDPAEVKITILEDKLTVEGERKREEEFREEDYYRSERVH
                     GSFLRSIQLPADVIGEKASAVYNGGVLTITVPKSRRPEAREIKVSVQEARPVEAPVSA
                     RQRRKKT"
BASE COUNT          364 a          605 c          521 g          483 t
ORIGIN      
        1 ctccgccgta cctgcccagc ctgtcgatgt tcctcaggtt gtccttcatg gtccgcgtga
       61 tgcccttcag gacctggtct cctgccaggt gtccgtaccg gtcattgatg agcttgaagt
      121 cgtccacatc gaagaggatg accgaaaagg gggagtcgta gcgtttcgcc cgttcgatct
      181 cggccccgag ctctcccacg atgcaggcat ggttgaaaac gcctgtcaac ccatcatgcc
      241 gggagagggt cctgatctct tcgaaaaggc ttatcttctc cagggtgagg gctatgtggg
      301 aacagaaacg ggtgaatatc gacgtctcct cttcgttgaa aggctcctgc ctgtatagcg
      361 cggcataccc gtaaagacgt tttgcggccc tgagctccga gaccaccgac gcggtgccgg
      421 gagagatctg tccgggcacc cggtttcccc agcctgcaag cgcggagacc attgaccgcc
      481 ggagttccgg atcgccgccg gtctcgggga agatgtgtat atcggcctcc tgcccgccca
      541 cgagcatggt accgaaaaac tggaaatcga ggaaacttcc gagcagcgtg tgcgcgtttc
      601 tgagcacgaa cacggggtcc tcggtctcgc ctatcatgcg ctggaggttc ttgaggaact
      661 cgagttcgag gatgttgatc ttttcgcggc ggaagcgctc ttcgacctgt gccgtcagat
      721 cctccgtcct gagtacctcc ctgtaatggt ccatgctgtt gttcacgatg tggtagagtt
      781 cctcaaagct cttgaagggt tttgtgagca gcgcgtaggc tccgttgctc agggattcaa
      841 gtctttccct ttcctccgcg taccccgtca tcattataac gggtatacgg cggtcgatct
      901 tttttatttc cttgaggatc tctattcccg agatgccggg gaggacgatg tcgcacagca
      961 cggcgtgagg cctcttgtcc ccgagctccg cgcgaaggtc gggcatgctc tggtaggtga
     1021 agacctcgag tccgaactgc tgcaggaatt cctgcagcac ggcaagaagc gtcacttcat
     1081 catcaatgat gatcagacgt ttttcaggtg acattctgaa ccggaccttt cagatccgtc
     1141 gatccaccct tcgactcatg cattgcgggg cccgtcaaac ggaaacgacc tctttcacct
     1201 ccgggatctc ctgtttgagc tgtttctcca cgcccatctt caaggtcatc atgctcatgg
     1261 ggcagcttcc gcacgcgccc tgcagcttga ccttcacgat gccgtcaacc acatcgataa
     1321 gctggatatc tcccccgtcc ctctgcaaaa ggggcctgat cttctctagc gcctgctgga
     1381 ctttctcttt catgtcttct tcctccgttg cctggcgctg accggtgcct cgacaggccg
     1441 cgcctcctgt acgctgacct ttatctctct cgcctccggc ctccgtgatt tgggtaccgt
     1501 gatggtaaga acacccccgt tgtatacggc cgaggccttt tcgccaatga cgtccgccgg
     1561 gagctggatg ctcctcagga aagatccatg aaccctttcg gagcggtaat agtcctcctc
     1621 gcggaactcc tcctcccgtt tccgttcacc ctcgacggtc agcttgtcct cgaggatggt
     1681 gatcttcact tccgccgggt ctatcgccgg tatgtccatg ataacaacga tttcgccttt
     1741 cctctccacg acgtcaacgg ccggaagcca gtcggcctcg cgaagcgtgg gaaacccggc
     1801 ttcgccgaag aattcctcga acacccggtc catctcttcc ctgagagagg gaaactttgt
     1861 ttcccacata gacttccacg gcgtcaaagg catctgatac ctcctgttat gaactttccc
     1921 ggtcggcggt ttctctcttc caaaagctga caatgcctga ctggtggaaa atg
//
LOCUS       DAOPOA010000490         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_62429_length_1969_cov_3.022466, whole genome shotgun sequence.
ACCESSION   DAOPOA010000490 DAOPOA010000000
VERSION     DAOPOA010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62429_length_1969_cov_3.022466"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1345)
                     /gene="nifS"
                     /locus_tag="PKJ17_09605"
     CDS             complement(200..1345)
                     /gene="nifS"
                     /locus_tag="PKJ17_09605"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOD76272.1"
                     /translation="MDNNATTPIHPRVADAVRPFLDGLFGNPSSLHFAGRDVKEPYET
                     AREQIAAIIGAKVNDVVITSCGSEADNHAIKGIAYGAGKGHIITSAVEHPAVLTCCRY
                     LETRGFPVTYLPVDGHGLVDPADVKDAIRKDTILISIMYANNETGTVMPIADIARVAR
                     EREIPFHSDMVQALGKIDVDVTALGVDLASFSGHKVYAPKGVGALYIREGLALDNLIH
                     GGHQESGRRAGTENVIGIVAFGRACELAREEMNERRETVEGLRRRLLNGILERIDQVR
                     LNGHPLQRVPNTLNLSFEFIESESLLLALDMKGVAVSSGSACSSGSSEPSHVLTAMGI
                     PGPLCQSALRFSLGCENSPADVDYVLDILPGLVEKLREMSPFYKKTF"
     gene            complement(1371..1781)
                     /locus_tag="PKJ17_09610"
     CDS             complement(1371..1781)
                     /locus_tag="PKJ17_09610"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HOD76273.1"
                     /translation="MKKRIILRFKKDTIDKPIVYRLVKDYNLVFNILRANISPRAESL
                     MVMEVEGTETNFKKAVRYLKDLNIDTEPMEQDINRDETRCVHCGLCTSVCAPGALYIK
                     DRKVMKVEFDYTRCVACEHCVKVCPVKAMNVSFD"
BASE COUNT          380 a          556 c          587 g          446 t
ORIGIN      
        1 acggaagaat cccactgatt ggccctgcag ccggtggtgt ggatgaaaaa ggtcgatttc
       61 ataataataa taaggacagt tttaggtttt aggttttaag tgaaagacga acagaaactg
      121 gcgggatgct gcggggcgac gcagcccgaa acaggaaccg gttcttcctg tttttcactt
      181 aaaacctaaa acgtaaaact taaaacgtct tcttataaaa cggtgacatc tcccgcagct
      241 tttccacgag gcccggaagg atatcgagga cgtaatccac gtcagcggga ctgttctcgc
      301 atcccaggct gaagcgcagc gcgctctggc acaggggccc cggtatgccc atggccgtta
      361 ggacatggga gggttccgag gagcccgacg aacaggccga accggacgat acggcaacgc
      421 ccttcatgtc gagggcaagc agaagcgatt ccgattctat gaactcaaag ctgaggttga
      481 gagtgttggg gaccctctgc aggggatgtc cgttgaggcg cacctgatcg atgcgctcca
      541 ggatgccgtt gaggaggcgc cgccgcaggc cttcgacggt ctcccgccgc tcgttcatct
      601 cctctctcgc cagttcacag gccctgccga aggccacgat gccgatgacg ttctccgtcc
      661 ccgcccgtct tcccgattcc tgatggccgc cgtggattag gttgtcgagg gcgagaccct
      721 cgcgtatgta cagggctccc acccccttcg gggcgtacac cttgtgcccc gagaaactgg
      781 cgaggtcgac gccgagggcg gtcacatcca catctatctt acccagggcc tgcaccatgt
      841 ccgaatgaaa gggtatctct ctctcgcggg cgacgcgggc gatatcggcg atgggcatca
      901 ctgtccccgt ttcgttgttc gcgtacatga tggagatgag gatcgtgtcc ttcctgatcg
      961 cgtccttcac gtccgccggg tcgacaaggc catgtccgtc gacgggaaga taggtgacgg
     1021 gaaagccacg ggtctcgagg tacctgcagc aggtgagcac ggcggggtgt tccaccgccg
     1081 acgttatgat gtggcctttg cccgccccgt aggcgatacc cttgatggcg tggttgtcgg
     1141 cctcgctgcc gcagctcgtt atgacgacgt cattcacctt cgcgccgatg atggcggcta
     1201 tctgttccct ggccgtttcg tagggctctt tgacgtcccg cccggcaaaa tggaggctcg
     1261 aagggtttcc gaagagaccg tcgaggaagg gcctcaccgc gtccgccacc cggggatgga
     1321 tgggggttgt agcgttgttg tccatgtata ttcggggtgt cggcacgttc ttaatcgaag
     1381 gagacattca tggcctttac ggggcagacc ttgacgcaat gctcgcaggc gacacagcgg
     1441 gtgtagtcga actccacctt catgaccttc ctgtccttga tgtagagggc gcccggggcg
     1501 cagacactgg tgcagaggcc gcagtgaacg caccgcgtct cgtcgcggtt gatatcctgc
     1561 tccatgggct ccgtgtcgat attgaggtcc ttgaggtacc tgacggcctt cttgaaattc
     1621 gtctccgtgc cctccacctc catgaccatg agggactctg ccctggggga tatgttggcc
     1681 ctcaggatgt tgaagaccag attgtaatcc ttgacgagcc ggtagacgat gggcttgtcg
     1741 atggtgtctt tcttaaagcg gagaatgatc cttttcttca tggagtcctc gcgtgtttcg
     1801 ttcgagccct gccggcggac tgacggatta tacttcgacg atgtcaagat ctccccaggc
     1861 gccgagctga tcgccgagta tgatcaatat ccccgttaca ccgggaaagc gctgtccttt
     1921 ttcgacggcg gcagcgatat cgtccttttt ttttacacta ttaccgagg
//
LOCUS       DAOPOA010000491         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_62565_length_1966_cov_3.003663, whole genome shotgun sequence.
ACCESSION   DAOPOA010000491 DAOPOA010000000
VERSION     DAOPOA010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62565_length_1966_cov_3.003663"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKJ17_09615"
     CDS             <1..232
                     /locus_tag="PKJ17_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76274.1"
                     /translation="IPAPETNHAIACAVDEAKRAKEEGKEKVILMNFSGHGVIDLPSY
                     DAFLSGKLQDYAMPDNEITELLRSLEKYPKPK"
     gene            552..1652
                     /locus_tag="PKJ17_09620"
     CDS             552..1652
                     /locus_tag="PKJ17_09620"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOD76275.1"
                     /translation="MLLSDRVMQMRPSGVRKIFDLAQKVKNPINLSIGEPDFDIPDVV
                     KDEGIKWIQKGFNKYTPSGGIPELREKVLGHLRGKGIVCEDAIITAGVTGGLLLALMT
                     TLNPGDEVIIPDPYFVLYEYQVLLLGGRPVFFDTYPDFTIREEELRRAITDRTRVILI
                     NSPNNPTGMVNSREELEMVARVAREKDLLVFSDDIYDRFVFDEEEGEKPYLGKMYEKT
                     LTFGGFSKTWGMTGWRLGYVAGPSEVIQCMITMQQYAFSSVTSFAQKAALVALDEGMD
                     DMIAGYRKKRDLIYNGLKDKYNVVKPKGAFFIFPEIPGGNAMAFVERALENNLFIIPG
                     SVFSARDTHVRISFAATEENLLKGIDVLRRMA"
     gene            1669..>1966
                     /locus_tag="PKJ17_09625"
     CDS             1669..>1966
                     /locus_tag="PKJ17_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76276.1"
                     /translation="MHENVIRLLMENAVQQIYVTNRGTAATARKVSGALHCEVSTNEK
                     IAFELALAGAYVRKRTACILSTEGLYEALDPVMSSAYTGVAGSFVVVAVQENDEE"
BASE COUNT          453 a          510 c          609 g          394 t
ORIGIN      
        1 catcccggcg ccggagacga accacgccat agcctgtgcc gtcgatgagg cgaaaagggc
       61 gaaggaagag ggtaaggaga aggtcatcct catgaacttc agcggccacg gtgtcataga
      121 cctgccgtcc tatgatgcct tcctgtcggg caagctccag gattacgcga tgcccgacaa
      181 cgagataacg gaactcctcc gcagcctgga gaagtatccg aaaccgaaat gaggcgggta
      241 atgggtgatg ggtgatggtt gcattcattg cctttcccat aacccatgat ccataaccta
      301 tcaccgaggt ttctatggag atctattgtg gtcagctggg gatgatcatc gggttcgatt
      361 attgtctccg ggtccagaac ggtttgccct gctccaacac ctttgggtgc tggaaagaaa
      421 gggttgacgt ggtcgctctt cttagggcaa agtttactga cgaagaattg cgattggctt
      481 tcaacacact gccgagatca aggctcgaca ggatacttaa gtccgcccgt tcagcttgca
      541 aggaggaata gatgctgctt tccgacaggg tcatgcagat gcggccatcg ggggtgcgaa
      601 agatcttcga cctggcgcaa aaagtgaaga accccatcaa cctcagcatc ggggaacctg
      661 atttcgatat tcccgatgtg gtgaaggatg aagggataaa gtggatacag aagggtttca
      721 acaagtacac gccctcggga gggatcccgg agcttcggga aaaggtcctc ggtcatctca
      781 ggggaaaggg gatagtctgc gaggacgcca tcatcacggc gggggtgacg ggaggtcttc
      841 ttctggccct catgacgacg ctcaaccccg gtgacgaggt catcatcccc gacccctact
      901 tcgttctcta cgagtaccag gtcctgctgc tgggagggcg gcccgtcttc ttcgatacct
      961 atcccgattt cacgatcagg gaggaggaac tgcgccgggc aataacggac aggacgaggg
     1021 tcatcctcat caacagcccc aacaatccga cgggcatggt caattcccgg gaagagctgg
     1081 aaatggtggc gagggtggcg cgcgagaagg acctcctcgt cttctccgac gatatctacg
     1141 accgcttcgt ctttgatgaa gaggaggggg agaagccgta cctgggcaaa atgtacgaaa
     1201 agacgctcac cttcggcggc ttctcgaaga cctgggggat gacgggttgg cgcctggggt
     1261 acgtggcggg gccgagcgag gtcattcagt gtatgataac gatgcagcaa tacgccttct
     1321 cgagcgtcac gtccttcgcg caaaaggcgg ccctcgttgc cctcgacgag ggcatggacg
     1381 acatgatcgc cgggtaccgt aagaaacggg acctcattta caacgggctc aaggacaagt
     1441 acaatgtggt taagccgaag ggggccttct tcatctttcc cgagattccc ggcggcaacg
     1501 ccatggcatt cgtggaaagg gcattggaga acaatctctt catcataccg ggcagtgttt
     1561 tttcggcaag ggacactcat gtgcgcatct ctttcgcagc caccgaggag aacctcctca
     1621 agggaatcga cgtactgaga agaatggcgt aaaggagttt tcggaacgat gcatgagaat
     1681 gtgatcaggt tgttgatgga gaatgccgtg cagcagatct acgtgacgaa caggggcacg
     1741 gcggcgacgg cgcggaaggt gtcgggcgcg ctgcattgcg aggtttcgac gaacgagaag
     1801 atagccttcg agcttgccct ggcaggcgcc tacgtgcgca agaggacggc ctgcatcctc
     1861 tccacggaag gtctctacga ggccctcgac cccgtcatga gctccgccta cacgggggtg
     1921 gccgggtcct tcgtcgtcgt ggccgttcag gagaacgacg aggagg
//
LOCUS       DAOPOA010000492         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_62613_length_1965_cov_2.638220, whole genome shotgun sequence.
ACCESSION   DAOPOA010000492 DAOPOA010000000
VERSION     DAOPOA010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62613_length_1965_cov_2.638220"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKJ17_09630"
     CDS             complement(<1..292)
                     /locus_tag="PKJ17_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOD76277.1"
                     /translation="MKEEDYRIERQEPGQPLIELQGITKVYGRGQAELHALRGIDLSI
                     YEGDFVAVMGPSGCGKSTCMNVLGCLDTPTSGRYLFNGVDIAGLTRNQRALLR"
     gene            complement(289..1548)
                     /locus_tag="PKJ17_09635"
     CDS             complement(289..1548)
                     /locus_tag="PKJ17_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD76278.1"
                     /translation="MMKILRSVGPGKGLKKRTIWLASAAAAILLAGAVVLYASTGKSQ
                     ARYKTAEVKKGDLTVTVTATGTLQPVEQVEVGTEVSGTIKTVAVDYNHKVKEGQVLAK
                     LDTTKLEAQVLQSEATLNYARAKLRETQATVAETQSKLNRFKESLELSGGKVPSQAEY
                     DAADAALKRAKAQEGTARADIAKAEATLKANRSDLNKATIRSPIDGVVLERKVEPGQT
                     VAASLQTPVLFKIAKDLTRMELCIGVDEADVGQVKEGQGAIFTVDAFPEKKFPARVRE
                     ARFAAKTENNVVTYETVLDVDNSDMVLRPGMTATAFITVNSVSDALLVPNAALRFAPA
                     QDKTQKENSGGSKLLTSLLSRRPPGADNRQAPRQGSGNVVWKVEGEKIAPVKVRPGAT
                     DGVMTQILEGDLAPGTLLATDIVREKK"
BASE COUNT          304 a          601 c          596 g          464 t
ORIGIN      
        1 gcctcaggag cgccctctgg ttgcgcgtga ggcccgctat gtcgaccccg ttgaagagat
       61 agcgtccgga tgtgggggta tcgagacaac cgagcacgtt catgcatgtc gatttcccgc
      121 atccgctcgg acccatgacg gcgacgaaat cgccttcgta gatggagagg tctatccctc
      181 tcagggcgtg cagctccgcc tgtcccctgc cgtatacctt cgttatcccc tgaagctcga
      241 taaggggttg ccccggttcc tgccgctcta tccgatagtc ttcttccttc atttcttttc
      301 cctcacgata tccgtcgcca ggagcgttcc cggggcaagg tcaccttcga gaatctgcgt
      361 cataacccca tccgtcgcgc ccggccttac cttcacgggg gcgatctttt cgccttcgac
      421 cttccacacc acgttgccgc tgccctgcct gggcgcctgt cggttgtccg ctccgggagg
      481 cctcctgctc aggagactgg tcaacagttt gctgcctccg gaattctcct tctgcgtctt
      541 gtcctgcgcg ggcgcgaacc tcagggccgc gttggggacc aggagggcat cggagacgct
      601 gttcaccgtg atgaaggccg tcgccgtcat gcccgggcga agcaccatgt cggaattgtc
      661 gacatcgagg accgtctcgt aggtcaccac gttgttctcc gtcttcgccg cgaaacgggc
      721 ctccctgacg cgggcgggaa atttcttctc cggaaaggcg tcaacggtga atatcgcgcc
      781 ctgcccttcc ttcacctgtc ccacgtcggc ctcatcgaca ccgatgcaca gttccatacg
      841 tgtcaggtcc ttggctatct tgaagagcac aggtgtctgg agggatgccg cgacggtctg
      901 gcctggctcc accttccgct cgaggacgac gccgtcgatg ggagaacgga tggtcgcctt
      961 gttgaggtcg gaccggttgg ccttgagggt ggcctccgcc ttggcgatgt ccgccctggc
     1021 cgtcccctcc tgggcctttg cccgtttcag cgcggcgtcg gccgcgtcgt attccgcctg
     1081 ggaaggcact ttcccgccgc tgagttcgag gctctccttg aagcggttca gcttgctctg
     1141 cgtctccgct accgtagcct gcgtctcgcg cagctttgcc cgggcatagt tcagtgtggc
     1201 ctccgactgc aggacctgcg cctcgagctt ggtcgtgtca agcttcgcca ggacctgccc
     1261 ctctttcacc ttgtggttgt agtccacggc gacagtcttg atcgtcccgg agacctcggt
     1321 gccgacctcg acctgctcta ccggttgaag cgtgcccgtg gcggtgacgg tgaccgtgag
     1381 gtcgcccttc ttcacttccg cggtcttgta gcgtgcctgc gactttcccg tggacgcgta
     1441 gaggaccacg gcacccgcca gaaggatcgc ggcggcggcc gaggcgagcc atatcgttct
     1501 cttcttcagt cccttgcccg ggcccaccga ccggagtatc ttcatcatat gtgacgtatc
     1561 gtcgcgagtc tcttccatgg atggctcctt ggtacatgag tttttggtct gcgatgtcgg
     1621 ttctttgatc atgactatgg agtcgtcggg agggaaggat cggttacaaa aaggtgaaga
     1681 tggcttgaac cgcttgaacc gtttcaacag aaacctgcgg gctcaggggt cttcgttgag
     1741 cttaaggagg atggaaggat atgtgtaacc tttttgctgc cgtctgcgac ttatggggaa
     1801 agatggaggc caggggcctt cgttgggatg acagggatgg agacgggatg ctcatttgag
     1861 tttacggcgg gagcgatggg gcgggaagat gcatgcccgc ccgagatgga acttgccctg
     1921 ccgggtcagc tttgttataa cagacctgta tgggtacctg ccgtg
//
LOCUS       DAOPOA010000493         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_62779_length_1961_cov_2.714586, whole genome shotgun sequence.
ACCESSION   DAOPOA010000493 DAOPOA010000000
VERSION     DAOPOA010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62779_length_1961_cov_2.714586"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKJ17_09640"
     CDS             complement(<1..841)
                     /locus_tag="PKJ17_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD76279.1"
                     /translation="MKAYNVINTRLPRVDARAKATGDARYTADLSMPNMLYGAMLQSP
                     LAHAKILNIDVTAAKKLPGVRDVVTWKEAGLVKYGVSPARYDEQVFCSDKVRYVGDEI
                     AAVAAVDLETAMEAVSLIKVDYEELPVLLDIESAMAEGAIAIHEDFPGNVSAEVHQEF
                     GNFEEALKECDIVRTDRFTNKRQDGGFLEPQACLAQYDLNGNLTLWSSTQSVHYVQRS
                     IAMVLGLPVGKVRVVKPYVGGGFGPKAAVNAMEIAACLMSKRTGRPVKMLFSREQVFL
                     HSRA"
     gene            complement(861..1331)
                     /locus_tag="PKJ17_09645"
     CDS             complement(861..1331)
                     /locus_tag="PKJ17_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOD76280.1"
                     /translation="MKKLITLTVNDTEYEVAVAPNKTLTQVLREDLNLLGTKEGCGVG
                     DCGACTVVLDGKPVNSCLVLAVQADGSRIKTIEGVAHGDSLHPVQETFVELGAIQCGF
                     CTPGMILSATSLLEKNPHPTEQEVRQALSGNLCRCTGYQKIVEAVQEASRRMKG"
     gene            complement(1331..>1961)
                     /locus_tag="PKJ17_09650"
     CDS             complement(1331..>1961)
                     /locus_tag="PKJ17_09650"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOD76281.1"
                     /translation="STFPVLARSASCLGSPLIRNRATIGGNIVTARPAADLPPALLVL
                     GARVELRGKDKDRTVSLDGFFTGPGTSVIETGEVLTRIVIPSPPPFTGADYIKLGHRA
                     ALEIAIVAVSSRLTLDKPDGVIVDARVILSSVAPTAIHAVSAEKALMGQRPSEDLFAK
                     AASLAVGDSSPITDMRGGAEYRRDMVNTLVKRTLLRAFKDARDTKGGNA"
BASE COUNT          338 a          638 c          580 g          405 t
ORIGIN      
        1 gggcccggga gtgcagaaag acctgttcgc gggaaaagag catcttcacc ggcctgcccg
       61 tccgtttcga catgaggcac gccgcgatct ccatggcgtt caccgccgcc ttgggaccga
      121 acccgccccc cacgtagggc tttacgaccc ggaccttgcc cacggggaga ccgagcacca
      181 tggcgatgct cctctggaca taatggaccg actgcgtcga cgaccagagc gtgaggttcc
      241 cgttgagatc atactgggca agacaggcct ggggttccag gaacccgccg tcctgcctct
      301 tgttcgtgaa cctgtcggtc ctgacgatgt cacattcctt caaggcctct tcgaaattgc
      361 cgaattcctg gtggacctcg gcgctcacgt tgccgggaaa gtcttcatgt atggcgatcg
      421 ccccttccgc catggccgac tcgatatcga gaagcaccgg aagctcctcg tagtcgacct
      481 tgatgaggga caccgcctcc attgccgttt cgaggtccac ggccgcgacg gccgctatct
      541 cgtccccgac atagcggacc ttgtcggagc agaagacctg ctcgtcatac cgggcaggac
      601 tgacaccgta cttcacgagt cccgcttcct tccatgtgac cacgtccctc acaccgggga
      661 gcttttttgc cgccgtcaca tcgatattca gtatcttcgc gtgcgccagg ggactctgca
      721 gcatcgcccc gtagagcatg ttgggcatgg aaaggtccgc cgtgtaccgg gcatcgcccg
      781 ttgccttcgc cctggcatcc accctgggca gccgcgtgtt tatcacgttg tatgccttca
      841 tagtccttgc ctcctcctcg tcatcccttc attcttctgg aagcttcctg caccgcctcc
      901 acgatcttct gataccccgt gcaccggcag aggttgcccg aaagggcctg tctcacctcc
      961 tgctctgtcg ggtggggatt cttctcgaga aggctcgtgg cggaaaggat cattccggga
     1021 gtgcagaaac cgcactggat ggcacccagt tccacgaatg tctcctggac aggatgaagg
     1081 gagtcgccgt gggccacccc ttctatcgtc tttatgcggg agccgtcggc ctggaccgcg
     1141 aggacaaggc aggagttcac cggtttgccg tcgaggacca ccgtgcacgc gccgcagtca
     1201 cccacgccgc atccctcctt cgttcccagg aggttcaggt cttcgcgcaa cacctgggtc
     1261 agggtcttgt tgggggcaac ggccacctcg tattccgtat cgttgactgt gagagtgatc
     1321 agctttttca tcacgcattc ccccctttcg tgtcccgggc atccttgaaa gccctgagca
     1381 gtgtcctctt tacgagggtg ttgaccatat cccggcggta ttcggctccg ccgcgcatgt
     1441 cggtgatggg agatgagtca ccgaccgcga gcgaggcggc cttcgcaaag aggtcttccg
     1501 acgggcgctg gcccatgagg gccttttccg ccgacaccgc gtgtatggcg gtgggcgcca
     1561 cggagctcag gatgacgcgt gcatcgacga tcaccccgtc aggcttgtcg agcgtcagtc
     1621 ttgaggacac cgcgacgatg gcgatctcaa gtgcggccct gtggccgagc ttgatatagt
     1681 cggcacccgt gaacggcggc ggggaaggta tgacgatccg cgtcagcacc tcaccggtct
     1741 cgatcaccga agttcccggt cctgtgaaga agccgtccag ggacaccgtt ctgtccttgt
     1801 ctttccctct aagctccacc ctggccccca gtaccagaag ggcgggaggg aggtccgctg
     1861 ccggccgtgc cgtcacgatg ttccccccga tggtggcacg gttcctgatg agcggagagc
     1921 cgagacagga cgcggaccgt gccagcaccg ggaaggtcga g
//
LOCUS       DAOPOA010000494         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_62978_length_1956_cov_3.559179, whole genome shotgun sequence.
ACCESSION   DAOPOA010000494 DAOPOA010000000
VERSION     DAOPOA010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62978_length_1956_cov_3.559179"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKJ17_09655"
     CDS             complement(<1..509)
                     /locus_tag="PKJ17_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HOD76282.1"
                     /translation="MKKKPLKERLLQGNEAVVEGALRAGCRFFAGYPITPATEIAEQL
                     SVRLPQVDGTFIQMEDEIASLGAVIGASLAGVKSMTATSGPGFSLMQENLGFAIIAEV
                     PCVVVNAMRGGPSTGLPTSPSQSDVMQARWGTHGDHPIIVLCPSTVRECFDLTIRAFN
                     YSEKYRIPV"
     gene            complement(466..753)
                     /locus_tag="PKJ17_09660"
     CDS             complement(466..753)
                     /locus_tag="PKJ17_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD76283.1"
                     /translation="MAGSRKRKASPKVDIYKAWCKACGICVAFCPTGSLARDEAGYPY
                     VKDPDKCINCGWCEIRCPDFAITVQQRKDEAKKVRESFEEETPEGTVAPGE"
     gene            complement(857..>1956)
                     /locus_tag="PKJ17_09665"
     CDS             complement(857..>1956)
                     /locus_tag="PKJ17_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76284.1"
                     /translation="TTSGDTAVPELLAMAKQPRGLLHVYELTAAEVEVATGSLRSELV
                     FKDLSTDFIRMEQFVNKLGEERHTGYIEIFGRDGRRLGTLSFRAGEPVVLQVLSESGQ
                     ATLFEGEAIPSVLENAVRNGAVFNVYRSSGCVTMDHPKAADTGPEDPDGSQRKGPASQ
                     ARARKEPKQAAEPAVQPEKEVKTADEPAVQRGKAVEDPAEPALREERVETPMEPPAPA
                     PKGATAGGQQVDDISANGRREFISALQRVLSKIESFIDHVSKKGDFQRVFRRVCVDAS
                     DLFHFLDPFEGQFEYDSGRIRVDDAIGSDDLAVATAYCLNLVLADLNREFLKGAALPP
                     GLKGEIESAFRHYKDAIKNSGMNFVVPANMR"
BASE COUNT          361 a          630 c          560 g          405 t
ORIGIN      
        1 atcacgggta tcctgtactt ctccgagtaa ttgaacgccc tgatggtgag atcgaagcac
       61 tcacggacgg tcgacgggca cagtacgatg atggggtggt cgccgtgagt cccccagcgg
      121 gcctgcatga catcgctctg ggacggggat gtgggcaaac cggtgctcgg cccgccccgc
      181 atcgcgttca ccacaacaca gggcacctcg gcgatgatgg caaacccgag gttctcctgc
      241 atgagcgaga aaccgggacc gctcgttgcc gtcatcgatt tcacaccggc gagcgaggcg
      301 ccgatcaccg cgccgagact cgcgatctca tcctccatct ggatgaatgt gccgtcgacc
      361 tgcggcagcc tgacggaaag ctgctccgcg atctccgtcg ccggcgttat gggataaccg
      421 gcgaagaacc ggcatccggc gcggagggct ccctcgacaa cggcctcatt cccctggagc
      481 aaccgttcct tcaggggttt cttcttcaaa ggactccctc acctttttcg cttcgtcctt
      541 cctctgttgt actgtgatgg caaaatcagg acaccgtatc tcgcaccaac cgcagttgat
      601 gcacttgtcc gggtctttga cataggggta cccggcctca tcgcgagcca gggatccggt
      661 gggacaaaag gcgacacaga tgccgcatgc cttgcaccag gctttgtaga tatcgacttt
      721 aggactagct tttctcttgc gcgatccagc cattagcaaa gtgacaatta cacagtagaa
      781 tgaacccgta ttcttgtcaa gcactttccg ggaggaccat gccggcccgg caggaggacg
      841 cgcgagcctc ccccgctcac ctcatgttgg ccggaaccac gaagttcata ccggagttct
      901 tgatggcgtc cttgtaatgc ctgaacgcgg actctatctc ccctttaagc cccggcggca
      961 gggcggcacc cttcaggaat tccctgttga ggtcggccag cacgaggttc aggcaatacg
     1021 ccgtggccac ggcaaggtca tcggaaccga tggcatcgtc aacacgtatc ctgcccgaat
     1081 cgtactcaaa ctgaccttcg aaggggtcca ggaagtggaa cagatccgac gcgtcgacac
     1141 agacccgcct gaagaccctc tgaaagtcgc ctttcttcga gacgtgatcg ataaaggact
     1201 ctatcttgga caacacccgc tgaagggcgg agatgaactc tctccggccg ttcgccgaga
     1261 tgtcgtcgac ctgctgtcct cctgctgtcg cacccttcgg cgccggcgcg ggaggctcca
     1321 tcggcgtctc cacccgttcc tcccgcaggg ccggttcggc cgggtcttcc actgctttcc
     1381 cccgctgcac tgcgggttcg tccgcggttt ttacctcttt ctccggctgc acggccggct
     1441 cggcggcctg tttcggttct ttcctggccc tggcctgaga tgccggaccc ttccgttgag
     1501 acccatcagg gtcctcagga cccgtatcgg ccgctttcgg gtggtccatc gtaacgcagc
     1561 cggagctgcg gtaaacgttg aacacggcgc cgttcctcac ggcattctcg aggaccgaag
     1621 ggatggcttc gccttcgaaa agggtcgcct ggccggattc ggagaggacc tgcagcacga
     1681 caggttctcc cgccctgaag gaaagggttc cgagccgtct gccgtccctt ccgaatatct
     1741 cgatgtaacc cgtgtgcctc tcttcgccca gcttgttgac gaactgctcc atgcggatga
     1801 agtcggtgct gaggtccttg aagacaagct ccgacctgag agaccccgtg gcgacctcga
     1861 cctcagccgc cgtgagctcg tacacgtgga gaagcccccg gggttgtttc gccatggcaa
     1921 gaagctccgg aaccgcggtg tctcccgacg ttgtcg
//
LOCUS       DAOPOA010000495         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_63132_length_1952_cov_4.018450, whole genome shotgun sequence.
ACCESSION   DAOPOA010000495 DAOPOA010000000
VERSION     DAOPOA010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63132_length_1952_cov_4.018450"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /gene="dnaG"
                     /locus_tag="PKJ17_09670"
     CDS             <1..1713
                     /gene="dnaG"
                     /locus_tag="PKJ17_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOD76285.1"
                     /translation="MKGSLDELLQRVNIIDVISQHVKLRKAGKDYMGLCPFHKEKTPS
                     FTVSVEKQIFYCFGCREGGNAINFIMKYENLTFVEALEHLGRQYGIEVERRGDRRKAG
                     HYDALGKLCEYYQKGLKSTSFALEYLARRGISREIADEFRLGYSVRSRGALKAALKAT
                     EIPADVFLSTGVVRMKETDLYDMFGGRIVIPIIDVNKRVIGFGGRTLEKDGIPKYVNS
                     PESSVFSKRTSLFGIDRTKKHITDRDEVYIVEGYFDLIGLYGAGITNAVSTLGTSVTE
                     GQIMKLRNYTENITLMLDGDEAGIKSALRLIGLFAEMDVNGNMVVLPEGHDPDSFVRE
                     KGAAAVAEVVKSKKPILDFYFDHYSGKEKMTTLQGKQAFIRQVMPHIDAIRDRVKRRL
                     YVKRLSELTGVEEEHFAGTRTYEVKGGAPRADAVKGVIERRVINVCMARPELLEYLKG
                     KEVLRYIRDDDVREVMTRMVTCFEQGKNLDVKNFIETLDKEDLKGLVLKAAFDEAGTD
                     PDEPEKVLLDYFRHIERQFFREKSKKITEKLAEAERSGDAAAVTELLEQKRQVLTHIK
                     NNFL"
     gene            1724..>1952
                     /locus_tag="PKJ17_09675"
     CDS             1724..>1952
                     /locus_tag="PKJ17_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76286.1"
                     /translation="MPEKVRRYYPYEENLENGELYAFISQGEKPSNILDDIDLFEIEH
                     QDAIGGAENADADGALDGDLEEVYDDDLINPI"
BASE COUNT          506 a          482 c          601 g          363 t
ORIGIN      
        1 atgaaagggt cactcgacga gcttctgcag agagtgaaca tcatcgacgt catatcccag
       61 cacgtgaagc tgagaaaggc cggaaaggat tatatgggcc tttgcccctt ccataaggag
      121 aagacgccct ccttcaccgt aagcgtcgag aagcagatct tctattgttt cggctgcagg
      181 gaaggcggca atgccatcaa cttcatcatg aaatacgaga acctgacctt cgtcgaggcc
      241 ctcgagcatc tcggccgtca atacggcata gaggtcgaaa ggagagggga caggaggaag
      301 gcaggccact acgacgccct ggggaagctc tgcgaatact accagaaagg ccttaagagc
      361 acgagcttcg ccctcgagta tctcgcgagg cgcggcatct cccgggagat cgcggacgag
      421 ttcaggcttg ggtacagcgt gcgttcccgc ggcgccttga aggcagctct caaggcgacg
      481 gagatacccg ccgatgtgtt cctcagcacc ggcgtcgtga ggatgaagga gacggatctc
      541 tacgacatgt tcgggggcag gatcgtcatc cccatcatcg atgtcaacaa gagggtcatc
      601 ggcttcggag ggaggacact ggaaaaggac ggcataccga agtatgtgaa ctcccctgaa
      661 tcatccgtgt tctcgaagcg cacgtccctc ttcggcatag accggacgaa gaagcacata
      721 accgatcggg atgaagtgta catcgttgag ggctacttcg atctcatagg cctgtacggg
      781 gctgggatca ccaacgctgt ctcgacgctg ggtacctccg tcaccgaggg acagatcatg
      841 aagttgagga attacacgga gaacatcacc ctcatgctcg atggcgacga agcgggcatc
      901 aagagcgcgc tgaggctcat aggccttttc gcggagatgg acgtcaacgg caacatggtc
      961 gtcttgccgg aaggtcacga ccccgacagc tttgtgcggg aaaaaggcgc cgccgccgtg
     1021 gcggaggtcg taaaaagcaa gaagcccatc ctcgacttct atttcgacca ttattccgga
     1081 aaagaaaaga tgacgaccct tcagggcaag caggccttca tcaggcaggt gatgccgcac
     1141 atcgacgcca tccgcgacag ggtcaagaga aggctgtacg tcaagaggct ttcggagttg
     1201 acgggggtcg aggaggagca ttttgcaggg acgaggacgt acgaggtgaa aggtggggct
     1261 ccgcgggcgg atgcggtgaa aggggtgatc gagagaaggg tcatcaatgt ctgcatggca
     1321 cgccccgagc ttcttgaata tctcaaggga aaggaggtat tgcgctacat cagggatgac
     1381 gatgtcagag aggtcatgac acgaatggtt acctgttttg aacagggaaa gaaccttgac
     1441 gtgaagaact tcatcgagac gcttgacaag gaggacctga aggggcttgt cctcaaggcg
     1501 gcctttgacg aggccgggac ggaccccgat gagcccgaga aggtgctcct cgattatttc
     1561 aggcacattg aacgccagtt cttcagggag aagtcgaaga agatcaccga aaagcttgcc
     1621 gaggcggaga gatcggggga cgccgcggcg gtcacagaac tcctggaaca gaagaggcag
     1681 gtattgacgc atataaaaaa taacttttta tagagaggtg gccatgcctg aaaaagtaag
     1741 gagatattat ccttatgaag agaatcttga gaacggggag ctgtacgcgt tcatatcgca
     1801 aggtgagaag cccagcaaca tccttgatga catcgacctt ttcgagatcg aacaccagga
     1861 tgctatcggc ggcgctgaga atgcggacgc cgacggggcc ctggatgggg accttgaaga
     1921 ggtctatgac gacgatctca tcaatcccat cc
//
LOCUS       DAOPOA010000496         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_63143_length_1952_cov_2.973643, whole genome shotgun sequence.
ACCESSION   DAOPOA010000496 DAOPOA010000000
VERSION     DAOPOA010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63143_length_1952_cov_2.973643"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PKJ17_09680"
     CDS             complement(<1..1023)
                     /locus_tag="PKJ17_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOD76287.1"
                     /translation="MMPLDTYEFNDVIHRCFRCGYCKFPTNWMDVNNCPPYARFRMES
                     YSCGGRLWLTRAWLNETLEWSEHLAEILYSCTTCRNCEVKCPLSFNVDIVNMVVAARS
                     EMIERGKVPPAVRRFLENIDLHGNPYGSARSRRDSWMQGTDIERYEGQEYLYYVGCTG
                     SYDTRAQRSARALGTLLGRAGVSFGVLGSEENCDGNEVHKLGEAGLFEMLAENNVERF
                     SSLGAKKIVTLSPHGYNAVKNLYPRYGGDFQVFHYTRLLLELIQEGRLPVSAGFDARL
                     TYHDPCFLGRWNDEYETPRRLLAAVPSLRLVEMEKNRDAALCCGGGAGNFEIDLLGGS
                     DASPARQ"
     gene            complement(1020..>1952)
                     /locus_tag="PKJ17_09685"
     CDS             complement(1020..>1952)
                     /locus_tag="PKJ17_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOD76288.1"
                     /translation="NALIHGQGDLAQPYGFNSDQVNGLEVVLPTGEVCRFGSCAIGAS
                     WFTNHPLPDVGLFFGWCGTTGLITKLSIRLYPAKRIRGVGQFVVEDEDLVPEIIGRVT
                     GTQMAEDVIAYSRAIPPFGLGLQHFTVNIAANSEKELEFKQELIWDDALGEYIRRGDG
                     GYLGFTRGLEHPQISKTSDYKKGGGFEYVGSIMPIEKYPECYRRGREISERHDIPYTV
                     TGRCVGVGHSMMFAWTYAFNRADPETMRQAREALHETDDLVLELGGVIWKPGTYGQQL
                     ILGRLDPGTRALMRRIKEVLDPGGIMNPGNWEGI"
BASE COUNT          365 a          606 c          568 g          413 t
ORIGIN      
        1 ctgtctcgcc gggctcgcat cgctgccgcc gagaaggtct atctcgaaat taccggcccc
       61 tcccccgcaa cacagggcgg cgtccctgtt cttctccatc tcgaccaatc gaagggaggg
      121 aacggcggcg agaagcctcc tcggggtctc atactcgtcg ttccatcggc ccaggaagca
      181 cgggtcatgg tatgtcagcc gcgcgtcgaa tccggccgag acagggagcc ttccttcctg
      241 tatcagctca aggagcaaac gggtgtagtg gaatacctgg aagtcccctc cataccgtgg
      301 gtagaggttt ttcacagcgt tgtagccgtg gggtgaaagg gtgacgatct ttttcgcgcc
      361 gagggacgaa aagcgctcaa cattgttctc tgccagcatt tcgaaaaggc ccgcctcgcc
      421 cagcttgtgc acctcgttgc cgtcacagtt ttcctcgctg cccagcaccc cgaaggacac
      481 gccggccctg ccgagcaggg tcccgagggc ccgggcggac ctctgcgccc gcgtgtcgta
      541 ggagcccgtg catccaacgt agtacaggta ctcctggccc tcgtatcgct ctatgtccgt
      601 cccctgcatc cacgagtcgc gcctgctgcg ggcgctgcca taagggttgc cgtggaggtc
      661 gatgttttcg aggaatctcc tcacggctgg cgggaccttt ccccgttcta tcatctcgct
      721 cctcgccgcc accaccatgt tgacaatgtc cacgttgaag ctcaatggac acttgacctc
      781 gcagttcctg caggtggtgc aggagtagag gatctcggca aggtgttccg accactcgag
      841 tgtctcattc agccacgccc gtgtcagcca gagccttccg ccgcaggaat aggattccat
      901 gcggaagcgg gcgtagggtg ggcagttgtt cacgtccatc cagttcgtgg ggaacttgca
      961 atagccgcat ctgaaacagc ggtggatgac atcgttgaat tcgtaggtgt ctaaaggcat
     1021 catattccct cccagtttcc cgggttcatg atgcctccgg ggtcgaggac ctctttgatg
     1081 cgtctcatga gcgcccgcgt gcccgggtcg aggcgcccga ggatgagctg ttgtccgtac
     1141 gtccccggct tccatatcac gccgcccagt tccaggacaa ggtcatcggt ttcgtgcagg
     1201 gcctcccgtg cctgtcgcat cgtctcggga tcggcccggt taaaggcgta ggtccacgcg
     1261 aacatcatcg agtgcccgac gccgacgcac cggcccgtca ccgtgtaggg gatgtcgtgt
     1321 ctctccgata tctcccggcc ccggcgatag cattcgggat atttctcgat gggcatgatg
     1381 cttcccacat attcgaagcc gccgcccttc ttgtaatcgg aggtcttgga tatctgggga
     1441 tgctcgagtc cgcgagtgaa tcccagatag cctccgtccc cccggcgaat atactcgccg
     1501 agcgcatcgt cccagatgag ttcctgcttg aactccagtt ccttctctga gtttgcggcg
     1561 atattaaccg tgaaatgctg aaggccgagg ccgaagggag gaatcgcacg gctgtatgct
     1621 atgacgtcct ctgccatttg agttcccgta accctcccga tgatctcggg aacgagatct
     1681 tcgtcttcca cgacaaactg accgacgcca cggatccttt tggcgggata gaggcggatc
     1741 gacagcttcg tgataagacc cgtggtcccg caccacccaa aaaagagacc tacgtcgggg
     1801 aggggatgat tggtgaacca cgaagcaccg atggcgcagg aaccgaagcg gcagacctcc
     1861 ccggtcggca gcaccacctc gaggccgttg acctgatcgg agttgaaacc gtaaggctgg
     1921 gcaaggtcac cctgtccgtg gatgagcgca tt
//
LOCUS       DAOPOA010000497         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_63151_length_1952_cov_2.506589, whole genome shotgun sequence.
ACCESSION   DAOPOA010000497 DAOPOA010000000
VERSION     DAOPOA010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63151_length_1952_cov_2.506589"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKJ17_09690"
     CDS             complement(<1..488)
                     /locus_tag="PKJ17_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOD76289.1"
                     /translation="MDIATLLGLIVGIGSVLAAFLMEGGHISALIQVPAMILVVFGTF
                     GAATVTTSVKQLINLPNLMKVAFSEKKMDSRQLIDLLFDLSQKARKNGLLSLEKDIDT
                     IKEPFLRKAIQLAIDGFETNKIREILEIEMAYIEERHKAGAAFFQKLGGFSPTLGIIG
                     TVL"
     gene            complement(495..1067)
                     /locus_tag="PKJ17_09695"
     CDS             complement(495..1067)
                     /locus_tag="PKJ17_09695"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOD76290.1"
                     /translation="MSGESERREFFRITDRIPLEFRPISRDEFERLEGIIRYKSTQVV
                     DRLNEVHFLECRGEPGEETDQMHAYMETINRKLDLILEILGGSSADSGYTAVKTDVNI
                     SGAGVQFVCEARLKEGDLVELKIIIPVFPYPRITCLCEVVRADRSDDAGSLRCALKFM
                     VINENDQDVLVNYIFLKERQYLRQKKETTS"
     gene            complement(1057..>1952)
                     /locus_tag="PKJ17_09700"
     CDS             complement(1057..>1952)
                     /locus_tag="PKJ17_09700"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOD76291.1"
                     /translation="YVPDGDTNELILYTGHTKDGEPPGWDWQRIGIPADLTGPARRTR
                     KHVVKDNRIYLPLVIQGEPIGFIVTEAKMNGRGPRSLENDAFFLRLIAEKASTQIENR
                     MLYESLFENILHTLRSLIIAITKRDLYTEGHCKRVTGMSLALGECLGVSDYEKDVIKV
                     VCPVHDLGKIGIPDSILLKPSRLSDEEYTIMQSHSVYGEEIMSRFEILAKEARVIRHH
                     HERYDGKGYPDRLAGEDIPMCSRIIAVCDAYDAMVTDRPYRKAMGADEIVAEIARCSG
                     SQFDPEIVKAFVDLVRDGYEW"
BASE COUNT          380 a          580 c          524 g          468 t
ORIGIN      
        1 agaacggtac caatgatgcc gagcgtcggg gagaaacctc cgagtttctg gaagaaggcg
       61 gccccggcct tgtggcgctc ctctatgtac gccatctcta tctcgaggat ctcccgtatc
      121 ttgttcgttt cgaaaccgtc aatggcaagc tgtatggcct ttcgcagaaa gggttccttt
      181 atggtgtcga tatccttctc gaggctcagg agcccgttct tcctggcctt ttgggacagg
      241 tcgaagagaa ggtcgatgag ttgccgggag tccatcttct tctcggagaa tgccaccttc
      301 atgaggttgg gaagattgat gagctgcttg acagaggtgg tcaccgttgc cgctccgaag
      361 gtgccgaaca cgacgagtat catggccggt acctggatca gcgcggagat gtgtcctcct
      421 tccatgagga aggcggcaag aactgaaccg attccgacta tgagtcccag gagggttgca
      481 atatccatag ctcatcaact cgtcgtctct tttttttgac gcagatactg gcgttccttc
      541 agaaaaatgt aattgaccag gacatcctgg tcgttctcgt ttataaccat gaatttcagc
      601 gcacacctga gggaacccgc gtcatcgctc ctgtccgccc tcaccacctc gcacaaacag
      661 gtgatcctgg ggtaggggaa cacggggatg atgatcttga gttccaccag atcaccctcc
      721 ttcaggcggg cctcgcagac aaactggacg ccggcgccgc ttatgttcac atcggtcttc
      781 accgcggtgt agccgctatc ggcggaagac ccgcccagga tctccaggat cagatcgagc
      841 ttcctgttga tcgtctccat gtacgcatgc atctggtccg tctcttcccc gggctctcca
      901 cggcattcca ggaagtggac ctcgttcagt ctgtcgacca cctgcgtgga cttgtagcgg
      961 atgatgcctt caagcctttc gaactcatca cggctgatcg gacggaattc gagggggatc
     1021 ctgtcagtga tcctgaagaa ctcccttctt tcggactcac cactcatagc catcccggac
     1081 gagatcgacg aaggccttca ctatctcggg atcgaactgg ctgccgctgc atcgcgcgat
     1141 ctccgccacg atctcgtccg cccccatcgc cttgcggtat ggacggtcag tgaccatggc
     1201 atcgtaagca tcgcacaccg ctattatgcg tgaacacatg gggatgtcct cgcccgcgag
     1261 acgatcggga taccccttgc cgtcataacg ctcatggtga tgcctgatga ccctggcctc
     1321 cttcgccagg atctcaaaac ggctcatgat ctcttccccg tacacggaat ggctctgcat
     1381 gatggtatac tcctcgtcgg agagtcgcga cggtttgagc aatatgctgt cgggaatgcc
     1441 tatcttcccg aggtcgtgga cagggcagac gacctttatc acgtcctttt cgtaatcgct
     1501 caccccgagg cactcgccga gggcaaggct catgcccgtg accctcttgc aatgtccttc
     1561 cgtgtagagg tcgcgcttcg taatggcgat gatcaacgat ctcagggtat ggaggatgtt
     1621 ctcgaagagg ctctcgtaga gcatcctgtt ctctatctgt gtcgacgcct tctcggcgat
     1681 gagacgaagg aagaaagcgt cgttctcgag ggaccgcggt cccctcccgt tcatcttcgc
     1741 ctccgtcacg atgaagccta tgggctcccc ctggatgacc agaggaagat atatcctgtt
     1801 gtccttcacg acatgctttc gcgttcgccg cgccggtccg gtcagatccg cgggtatgcc
     1861 gatgcgctgc cagtcccatc ccggcggctc tccgtccttc gtatgtcccg tgtaaagtat
     1921 cagttcattc gtatccccgt cgggcacata gt
//
LOCUS       DAOPOA010000498         1950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_63220_length_1950_cov_3.419525, whole genome shotgun sequence.
ACCESSION   DAOPOA010000498 DAOPOA010000000
VERSION     DAOPOA010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63220_length_1950_cov_3.419525"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PKJ17_09705"
     CDS             <1..460
                     /locus_tag="PKJ17_09705"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOD76292.1"
                     /translation="TMVLMLIAGSTVLGHFIAVTTIPMVAADWIVSLNMPSWVIIVFI
                     ALIYLIGGSFIDDLAFMILATPIFYPAILKLGYDPMWFGILIGITVMLGVVLPPMAIC
                     VFVVKQITKTPFSVVYAGVYPFLISIIVGGILVFIFPALSTWLPHWLMPG"
     gene            complement(996..1448)
                     /locus_tag="PKJ17_09710"
     CDS             complement(996..1448)
                     /locus_tag="PKJ17_09710"
                     /inference="COORDINATES: protein motif:HMM:NF017999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOD76293.1"
                     /translation="MIIEGGFTIKAPIDKLFDFLLKPETILTCLPGAESANMIDDTSY
                     ECIVKQKVGPIKARLKFVNKMTVVNKPTHIEIEGEGEDMTKLGHFKQKTVVDLTEKGN
                     GEVEVTYKTDVSIVGKLAMFGDRIMRAKAKDTEKEFTKNLQEKLKSIA"
     gene            complement(1503..1718)
                     /locus_tag="PKJ17_09715"
     CDS             complement(1503..1718)
                     /locus_tag="PKJ17_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76294.1"
                     /translation="MSFKCPACKKEWPNSKQVARHMFGTGDKAHRAWIESQGYSYIEL
                     LLAQTTEPGNKSYEILADLIEKAQDKL"
     gene            complement(1749..>1950)
                     /locus_tag="PKJ17_09720"
     CDS             complement(1749..>1950)
                     /locus_tag="PKJ17_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76295.1"
                     /translation="TDIIEIDTYEPEGPYGAKEAGEGLTNPTAAALGNAVFNALGIQM
                     KELPITPERVRKALAEKKEAQK"
BASE COUNT          328 a          592 c          483 g          547 t
ORIGIN      
        1 caccatggtc ctcatgctca tagccggctc cactgtcctc ggtcatttca tcgccgtcac
       61 gacgataccc atggtcgccg cggactggat cgtcagcctc aacatgccct cctgggtcat
      121 catcgtcttc atcgccctca tctacctcat cggcggttca ttcatcgatg acctggcctt
      181 catgatcctg gcgacgccca tcttctaccc cgcgatactc aaactcggat acgaccccat
      241 gtggttcggc atcctcatcg gcataacggt catgctcggc gtcgttctcc ctcccatggc
      301 catctgtgtc ttcgtcgtca agcagatcac caagacaccc ttcagcgtcg tctatgccgg
      361 cgtctacccc ttcctcatca gcatcatcgt cggcggcatc ctggtcttca tcttccccgc
      421 gctttcgacc tggctccccc attggctgat gccgggctga caaaaaagga ttgaaggcaa
      481 aaaccgcctc cctcaggagg cggttttttt tctccaatat cctcctgata tagttctctt
      541 ttacatcatt ccgcctatcg tcccctcctc ctctctgcac tccccattcc ccgtcatccc
      601 gccattcttc cttctccgtc attccggcgc aggccggaat ctaggaaggc ctggcaatga
      661 atgaacgctg aaaggttcga tggcacctct cgtatcgtgc tcttcggtca gtatccttat
      721 gtttgctttc tgccgcaacc gagtttttcc tggttcccgg cctgcgccgg gatgacggac
      781 tgagagtgtg gagggatgat gacgaagggc ggggcgggaa acggggtttg tcaacagcct
      841 gtcgagcggt gcaagtgaaa gacggctaga cccgacggcc tctgaacacg attggcacac
      901 cggtggaagg tgcccgttgc cttgttttgt cttttggtaa cgaaaaaggg gttcccgcac
      961 agggcgggaa ccccttttct tcttcgcatt atttactacg ctatggattt cagcttttcc
     1021 tgaaggttct tcgtgaattc cttttccgtg tccttcgcct tcgccctcat gatcctgtcg
     1081 ccgaacatgg cgagcttgcc gacgatgctc acgtctgtct tgtatgtgac ctcgacctcg
     1141 ccgttgccct tttctgtcag gtcaacgacc gtcttctgtt tgaagtggcc gagtttggtc
     1201 atatcttcgc cctcaccttc gatctcgatg tgcgtgggct tgttgacaac ggtcatcttg
     1261 ttcacgaatt tcagcctggc cttgatgggc ccgactttct gcttcacgat gcactcgtat
     1321 gacgtgtcgt cgatcatatt cgccgactct gcgcccggaa ggcaggtgag gatcgtttcc
     1381 ggtttcagca ggaaatcgaa gagcttatcg atcggtgctt ttatggtaaa accgccctct
     1441 atgatcatgg gacttcctcc ttaatgttat aattatcaag cctttcgcgg gctcaattat
     1501 tcttacagct tgtcctgcgc cttctcgata aggtccgcaa ggatctcgta gctcttgttg
     1561 cccggctcgg ttgtctgagc gaggaggagc tcgatatatg agtaaccctg agattcgatc
     1621 catgcccggt gcgccttgtc gccggtaccg aacatgtggc gcgccacctg cttgctgttg
     1681 ggccattctt ttttacatgc gggacattta aagctcacgg tatacctcct tatttgtctt
     1741 tgtcagggct atttctgggc ttccttcttc tcggccaggg ccttgcggac cctttccggg
     1801 gtgatgggaa gctctttcat ctggatgccc agggcgttga aaacagcgtt gccaagcgcg
     1861 gccgccgtcg ggttcgtcag gccttcgccg gcctctttcg cgccgtaggg tccttccggc
     1921 tcgtaggtat cgatctcgat gatatccgtc
//
LOCUS       DAOPOA010000499         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_63303_length_1948_cov_3.199683, whole genome shotgun sequence.
ACCESSION   DAOPOA010000499 DAOPOA010000000
VERSION     DAOPOA010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63303_length_1948_cov_3.199683"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="PKJ17_09725"
     CDS             <1..1151
                     /locus_tag="PKJ17_09725"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD76296.1"
                     /translation="GRVRELKSVWPSRKNGADIIAEMENELKARDNVAIFTSSKLTNF
                     EGFFGNYQATIDTPNGEKKVMAGGVVVAIGFTPFDPDIKPELGYGRDPRIVTTLDLER
                     MADALKLPDKPRVAVLHCIGSRDEQIGRPYCSRICCINALRAAEGIKERYPDSYVESF
                     YMDMRAHPRGGEEFFEDTQEKGVLFTRANIAEIIPEPEGVVLHGEDTLLDEVFERGFD
                     LVVLSIGMSSPATSRDIASLLKISLDKDKFFLEAHIKLRPFDTAVKGIFIAGSCSGPK
                     DVEESISHGRASALKLYGFLNLGYAFVDPFISIVDPKRCSGCRMCEQACVAKAIKFDE
                     DKHVVHVEEAACMGCGLCNATCPSSAISLKGYVDTIIGDEISALLEAM"
     gene            1174..1581
                     /locus_tag="PKJ17_09730"
     CDS             1174..1581
                     /locus_tag="PKJ17_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOD76297.1"
                     /translation="MEQKIEKNPEIVGFACSFCTFKASEMAGSLRMKYPEGVKLIQVP
                     CSSRVDPAFVIKAIFEGADGVFVAGCHPGDCHFIKGNYFTRRKIAALKEELEAFGMKD
                     KLRLFWVSAAEARRFVEKVTEMYNDIKEKKNGR"
     gene            1571..>1948
                     /locus_tag="PKJ17_09735"
     CDS             1571..>1948
                     /locus_tag="PKJ17_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76298.1"
                     /translation="MVGKKITNGQIEGALNKLLSGGDCLVMGFEKDSAGIRHRVFKDS
                     LGNMSITNHIFALNGGLYLRRLFSKGARIALVLRPCEIRSYVELVKLSQVEPENIIAV
                     SVDCFGTISSKVDGVPVDPDADVL"
BASE COUNT          451 a          505 c          601 g          391 t
ORIGIN      
        1 gtggccgcgt ccgggagctg aaaagcgtct ggccgtcgcg taagaacggg gccgatataa
       61 tcgccgaaat ggagaatgag cttaaggctc gggataatgt cgccatattc acctcgtcga
      121 agctcacgaa tttcgaaggt tttttcggga attaccaggc aaccatagac accccgaacg
      181 gcgagaagaa ggtcatggct ggaggggtgg tcgtcgccat cggttttaca ccgttcgatc
      241 ccgacataaa gccggagctc ggctatggca gggacccgcg catcgtaacg accctcgacc
      301 tcgaacggat ggctgacgcc ttgaagctcc ccgataagcc gagggttgcg gtcctgcatt
      361 gcatcgggtc ccgggatgag cagatcggca ggccttactg ctcgcgcata tgctgtatca
      421 atgctctccg ggcggcggaa gggataaagg agagatatcc ggattcgtac gtggagtcct
      481 tttacatgga catgcgcgcc cacccgcgcg ggggcgagga attctttgag gacacgcagg
      541 aaaaaggcgt tctcttcacg cgtgcgaata tcgcggagat catccccgaa cccgagggag
      601 tcgtacttca cggtgaggat acgctcctcg acgaggtatt cgaacgtgga ttcgaccttg
      661 ttgtcctgtc gatcgggatg tcctccccgg ccacgtcgag ggacatcgca agccttctca
      721 agatctctct cgacaaggac aagttcttcc tcgaggccca catcaaactg cgtcctttcg
      781 atacagccgt gaaaggcata ttcatagcgg gctcctgttc ggggccgaag gatgtcgagg
      841 aatcgataag ccacggaagg gcatccgccc tgaaactata cggcttcctc aacctgggat
      901 acgccttcgt cgacccgttc atctcgatcg tcgacccgaa gcggtgcagc ggctgcagga
      961 tgtgcgagca ggcgtgcgtg gcgaaggcaa taaagttcga tgaagataaa catgtcgtgc
     1021 acgtcgaaga ggcggcgtgc atgggctgcg ggttgtgcaa cgcgacgtgc ccgtcgtccg
     1081 cgatcagtct gaaaggctac gtggacacga taatcggtga cgagataagc gcgctcctgg
     1141 aggcaatgtg aataaaccgg cagaggtatt gtgatggaac agaagatcga aaagaacccc
     1201 gagatcgtcg gtttcgcctg ttcgttctgc acgttcaagg cgtccgagat ggcgggaagc
     1261 ctgaggatga aatatcccga gggggtgaag ctcatccagg tcccttgttc gagcagagtc
     1321 gacccggcct tcgtcatcaa ggcgatattc gaaggggctg acggtgtttt cgttgcggga
     1381 tgccatcccg gcgactgcca tttcatcaag gggaactatt tcacccgcag aaagatagcg
     1441 gcactgaaag aggaactcga ggcgttcggc atgaaggaca aactccgcct cttctgggta
     1501 agcgcggccg aggcgcggcg cttcgtagag aaggtcacgg aaatgtacaa cgacataaag
     1561 gaaaagaaaa atggtcggta agaagatcac gaacggtcag atcgaaggag ccctcaacaa
     1621 gctcttgagc ggcggagact gcctcgtcat gggtttcgag aaggacagcg cagggatccg
     1681 gcaccgggtt ttcaaggata gccttgggaa catgtcgatt acgaaccata tctttgccct
     1741 gaacggcggg ctttacctaa ggagactctt ttcgaagggg gccaggatag ccctcgtgct
     1801 ccggccatgc gagatacggt cgtatgtcga attggtcaag ctctcccagg tcgagccgga
     1861 gaatatcatc gcggtgtccg ttgactgttt cgggacgata tcatccaaag tggatggcgt
     1921 gccggtcgac ccggatgccg atgtcctc
//
LOCUS       DAOPOA010000500         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_63570_length_1942_cov_5.864865, whole genome shotgun sequence.
ACCESSION   DAOPOA010000500 DAOPOA010000000
VERSION     DAOPOA010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63570_length_1942_cov_5.864865"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKJ17_09740"
     CDS             complement(<1..238)
                     /locus_tag="PKJ17_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD76299.1"
                     /translation="MRPATEVLLTEYLKQLKLTAILRVYAARAREARNTGSDYEDYLL
                     SLMESELEVRADNRLKRRIRDARFPVMKTMEGFNF"
     gene            complement(235..1722)
                     /gene="istA"
                     /locus_tag="PKJ17_09745"
     CDS             complement(235..1722)
                     /gene="istA"
                     /locus_tag="PKJ17_09745"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOD76300.1"
                     /translation="MDQYEYIRTAHRVYGKSIRQIQKETGHSRVTIRKILEGEFSEYK
                     RRSVQAYPVLGKHTDTIERWLKEDREIPKKQRHTARRIYTRLVEEEGYAGSEVTVRRY
                     VRQVKAKEGMDTSRAYLILEPECGQEAEVDWGEAIAMVRGIRTPFHFFCMRPRFSGRP
                     FVRAYPCERQQAFFDAHAHAFTFFGGVFPVLVYDNLRSAVEKVLTGRNRIEQDAFRKL
                     RSYYNFEARFCNPGSANEKGGVEGVIGYVRRNFLVPVPVVDSFEELNAHLLRSCLRHG
                     SHRIAGRTENIDSLFEREKGCLIPLPAVPLANITLLETNVDKYSTVVVDKNRYSVPVS
                     YAWLKVRVELSIDGVDIFHDGRRIAHHERLFGNNKWQLDPQHYLELVRRKPGAFDSAL
                     VIRRWREEWPACLEKLLARFRQKQGDTPGIKDFISVLMLYRDYPPEDIEAVVELALEN
                     SVSASDGIRHMLVYSGPEEHFEPLQGWPATPVPDMTRYAGLGVIR"
BASE COUNT          356 a          580 c          575 g          431 t
ORIGIN      
        1 caaaattgaa gccctccatg gtcttcatga ccgggaaccg cgcatccctg atccgccgtt
       61 tgagacggtt gtcggcacgg acctcgagct ccgattccat gagcgacagc aaatagtcct
      121 catagtccga acccgtgttc cgggcctcgc gtgcccgggc ggcatagacc ctcaggatgg
      181 cggtcagctt gagctgtttg aggtattccg tgagaagcac ctccgtggcc ggcctcatcg
      241 tatcaccccc agcccggcgt accgggtcat gtccggaaca ggcgttgccg gccagccctg
      301 aaggggctca aaatgctctt ccggaccgga gtagacgagc atgtgtcgta tcccgtcgct
      361 tgcggagaca gagttctcga gggcaagctc caccacggcc tcgatgtctt caggcgggta
      421 gtccctgtag agcatgagca cggatataaa gtcctttatc cccggtgtgt ccccctgctt
      481 ctgcctgaac cgtgcgagga gcttctcgag gcacgccggc cactcttcac gccaccggcg
      541 gatgacaagg gcgctgtcga aggcgccggg cttgcgtctt accagttcca ggtaatgctg
      601 gggatcgagc tgccacttgt tgttcccgaa gagcctctcg tggtgtgcta tcctcctgcc
      661 gtcatggaag atgtcgaccc cgtcgatgga cagctctacc cggaccttta accaggcata
      721 cgagaccggt acggagtagc ggttcttgtc gacgacgacg gtcgagtact tgtcgacgtt
      781 cgtttcaaga agggtgatat tggcaagcgg tacggcggga agagggatga gacatccttt
      841 ttctctttca aagaggctgt cgatgttctc cgtgcgcccg gcgatccggt gggagccgtg
      901 gcgaaggcag gagcgcagaa ggtgcgcgtt gagctcctcg aagctgtcaa cgacggggac
      961 gggaacgagg aagttgcgcc tcacataccc gatcacgcct tccaccccgc ccttctcgtt
     1021 ggcggatccc gggttgcaga agcgggcctc aaaattgtag taggacctca atttacggaa
     1081 ggcgtcctgt tcgatgcgat tccggccggt caggaccttc tcgacggcac tcctcaggtt
     1141 atcatacacg aggacgggaa agaccccgcc gaagaaggtg aaggcatggg catgggcgtc
     1201 gaagaaggcc tgctgcctct cacagggata ggcacgtacg aagggtctgc cggagaacct
     1261 ggggcgcatg cagaagaagt ggaagggcgt ccgtatcccc ctgaccatgg cgatggcctc
     1321 tccccagtcg acctccgcct cctgtccgca ttcgggttcc aggatgagat atgcgcggga
     1381 cgtgtccatc ccttcctttg ccttcacctg gcggacgtac cggcgcacgg tcacctcgct
     1441 tccggcgtat ccttcctcct cgacgaggcg cgtgtagatc cggcgggcgg tgtggcgctg
     1501 ctttttcggg atctcgcgat cttccttcag ccagcgctct attgtgtccg tgtgcttccc
     1561 gaggacgggg tatgcttgta ccgatcgccg tttatactcg gagaactcgc cctcgaggat
     1621 cttcctgatc gtcacccggg aatggcccgt ctccttctgt atctgccgga tgcttttgcc
     1681 gtaaacccgg tgcgccgtcc tgatgtactc gtactgatcc atcgttatca ccctttccct
     1741 ccggtacagt cggtcttgtc agaccaactt accagagatc gttccggggg tgggtcattt
     1801 ttcggtgatc atttgcccgt tgggtgggtc agttttaggt tatcacaacc acctctcgaa
     1861 ggagatgacg aagaaaggcg tcacccttgt caaggaccag gttaattccc cactaccggg
     1921 gccaaggtaa ttccccactt tt
//
LOCUS       DAOPOA010000501         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_63851_length_1936_cov_3.522594, whole genome shotgun sequence.
ACCESSION   DAOPOA010000501 DAOPOA010000000
VERSION     DAOPOA010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63851_length_1936_cov_3.522594"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PKJ17_09750"
     CDS             complement(<1..280)
                     /locus_tag="PKJ17_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76301.1"
                     /translation="MSESNSRKWIDSKELLTRSAISRATLNNYIKLGVLPRPVVKSPA
                     DSQKGTKKIGYFPDTVLDTISEIKKLKEKGKPMEAIAREMRTSSPESGY"
     gene            complement(530..808)
                     /locus_tag="PKJ17_09755"
     CDS             complement(530..808)
                     /locus_tag="PKJ17_09755"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOD76302.1"
                     /translation="MKIDEKLALDAFKTDRESHILIDHDICRSRCTLRHCLNICPGHL
                     YSFNEEGNEIVVEYAGCLECGTCMVACTEGAIQWSYPRGDFGVQYRYG"
     gene            complement(820..>1936)
                     /locus_tag="PKJ17_09760"
     CDS             complement(820..>1936)
                     /locus_tag="PKJ17_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76303.1"
                     /translation="EMLAGAPFERKVVRERWSLLGSTNSVSIDHTSDRFRDEDHSYTV
                     LRARLDRWLSERLMARGVFVVPKYRVDDLLWENGAVAGIRAGAEEIPAHVVVAADGVL
                     SFMGRKAGLMPPLSPKTCAVGMKEVLELSEEKINDRFNVGDGEGVAHLFLGDVTKGVF
                     GGGFLYTNKDTVSLGVVAGIGAMMGRIPAMDASTLLDMFKERYEMRRLLEGAKIVEYS
                     AHVIPEAGYDGIGKLCGNGILLAGDAAGLALNMGVTVRGMEFAIASGMAAAEAIVEAK
                     ASGDLSEKGLSRYGERLKETFVLKDLEASRNMPGYIDNGSFFSYYPETFPGLLEKIMW
                     FDESPKGSLGKTLWKELRSSGMLSVKRLIELYRIRNI"
BASE COUNT          405 a          589 c          516 g          426 t
ORIGIN      
        1 cgtaaccgga ctccggggag gacgtcctca tttctctggc tatggcctcc atgggtttgc
       61 ctttttcctt gagcttcttg atctcgctga tggtgtctaa gacagtatca ggaaaataac
      121 cgatcttctt cgtacccttc tggctgtccg ccgggctctt cacgacgggc ctcggaagaa
      181 cgccgagctt gatgtagttg ttgagggttg cccttgatat cgctgacctg gtcagaagct
      241 ccttactgtc tatccatttg cggctgttgg actcactcat ccactttccc cccgtttaca
      301 ccgtaaacta accagcaatt tacatactta tacagtacag aacagttgtc aagaaaataa
      361 ttagacagta caactaggga gagtacatat ttaaggctta aggagacagg aaacggggct
      421 tgtcgcgcgc ggggcttacc gatccaggaa ttcgatcacg gcaaggtcgc gaagagtgga
      481 agaaagctca ttttcctgga acctgagata tgaaacctgc aaccggcttt catccgtacc
      541 ggtactgcac gccaaaatcc cccctggggt agctccactg gatggcgcct tcggtgcagg
      601 ccaccatgca cgtgccgcat tcaaggcatc ccgcgtattc cacgactatc tcattgccct
      661 cttcgttgaa ggaatacaga tgcccggggc agatgttcag gcagtgtcgc agggtgcatc
      721 tggacctgca gatatcgtgg tctatgagga tgtggctctc cctgtccgtc ttgaaggcgt
      781 caagggcaag cttttcgtct atcttcatgg cctgtcacct caaatattcc tgatgcggta
      841 aagctcgatg agtcgtttca cgctcagcat acccgatgag cgaagctcct tccacagggt
      901 ctttcccagg gaacctttcg ggctctcgtc aaaccacatg atcttctcga ggagcccggg
      961 gaaggtctcg ggatagtagg agaagaatga gccgttgtcg atgtatcccg gcatgttcct
     1021 cgatgcctcg aggtccttga ggacgaaggt ctccttgaga cgttccccgt agcgggaaag
     1081 gcccttttcc gaaaggtcac cggacgcctt cgcctcgact atggcctcgg cggcggccat
     1141 gccggaggca atggcgaatt ccatcccccg gaccgtcaca cccatgttga gggcgagacc
     1201 cgcggcgtcg ccagcgagga gaatgccgtt gccgcagagc ttcccgatgc cgtcatagcc
     1261 ggcctcgggt atgacatgcg ccgaatactc gactatcttc gcgccctcga ggagtcttcg
     1321 catctcgtag cgctccttga acatatcgag gagcgtcgac gcatccatcg ccggaatcct
     1381 tcccatcatg gcgccgatac ccgccaccac acccagcgaa acggtatcct tgttcgtata
     1441 gagaaaaccg cccccgaaga cacccttcgt cacatcaccg aggaacagat gggcaacgcc
     1501 ttccccgtca ccgacgttga aacggtcgtt tatcttctct tcggaaagct cgagcacctc
     1561 tttcattccc acggcacacg tcttcggaga aaggggaggc atcaaccccg ccttgcgtcc
     1621 catgaaggag aggacgccat cggcggccac gacgacgtgc gcggggatct cttccgcccc
     1681 ggcgcgtatc ccggccaccg ctccgttctc ccacaggaga tcgtcgacgc ggtatttggg
     1741 gaccacgaaa acgcccctcg ccataaggcg ctccgaaagc cacctgtcga ggcgggccct
     1801 gagcaccgtg tagctgtggt cctcatccct gaaccggtct gaggtgtggt cgatactgac
     1861 ggaattggtg cttcccagaa ggctccagcg ttcgcgcacg accttgcgct caaaaggcgc
     1921 cccggcaagc atctcc
//
LOCUS       DAOPOA010000502         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_63991_length_1933_cov_2.783280, whole genome shotgun sequence.
ACCESSION   DAOPOA010000502 DAOPOA010000000
VERSION     DAOPOA010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63991_length_1933_cov_2.783280"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="PKJ17_09765"
     CDS             <1..1334
                     /locus_tag="PKJ17_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76304.1"
                     /translation="LTHLVRQAYRDIEENITGMHPSVYRQVRAGAEVAVIALPKDTGL
                     PGGSYRKLRDVPMIRQILLYPPLLLNPPMNKRTGKFERIPQNPLELMEIAPDEWLCYP
                     AKVGPLLVLVYVHEKFYELGLSLCNLFELADDEFLDRPVDAVYLFGVPSGALDGLAPM
                     PTVFCDDEQNGLLVAACPNEDQFGYFGYLKKMVLTLHNVKVMKLGRMPYHGAMVRIVT
                     KGDRSLTLLIIGDTGAGKSETLEAFRTMGEDRIEEMTIIADDMGSLDIGQEGSVIGYG
                     TEVGAFVRLDDLQPGYAFGQLDRSIIMNPSQVNSRIVLPVTTYENVMEGCSVDMVLYA
                     NNYEETDEEHPIIERLNTPEGALHVFREGTAMSKGTTTSVGLVHTYFVNVFGAVQYKA
                     LHDDIARRFFRAFFEKGIFVGQLRTRLGIPGWERKGPEQAAQALLGMIADR"
     gene            complement(1518..>1933)
                     /locus_tag="PKJ17_09770"
     CDS             complement(1518..>1933)
                     /locus_tag="PKJ17_09770"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOD76305.1"
                     /translation="PDTTDAYTHRIGRTGRAAKTGDAFTFVCQEDEPQVRAIERVLGA
                     RIERRTVKDFDYTTPAPERNVEFARPPRPQRRDRAQVKPERAGTGKEDNKKPGTGSPG
                     SARAGLCASGPKPAGPSTQRHRRKRTGPSSKRRGG"
BASE COUNT          353 a          574 c          623 g          383 t
ORIGIN      
        1 cattgaccca tctggtgcga caggcttacc gggacataga ggagaacatt accggtatgc
       61 atcccagtgt ctaccggcag gttcgggcgg gagcggaagt ggccgtgatc gcgctgccaa
      121 aggacaccgg attgcccggc gggagttatc ggaagctgcg cgacgttccc atgatccgcc
      181 agatcctgtt gtacccgccg ctccttctga atcctccgat gaacaagcgg acagggaaat
      241 tcgagaggat accccagaac cccctggagc tgatggagat cgctcccgac gagtggctct
      301 gttaccctgc caaggtcggt ccgctcctcg ttctcgtgta tgtgcacgag aagttctacg
      361 agcttggcct gtcgctgtgc aacctgttcg agctggccga cgacgagttc ctcgatcgcc
      421 ctgtcgacgc ggtctacctt ttcggagtgc cttccggcgc cctcgacggc ctcgcgccca
      481 tgccgaccgt cttctgcgac gatgaacaga acggcctcct ggtagccgcc tgtccgaacg
      541 aagaccagtt cgggtacttc ggatatctca agaagatggt gttgacgctc cacaacgtca
      601 aggtcatgaa actggggaga atgccctatc atggtgccat ggtcaggatc gtgacgaaag
      661 gcgaccgttc tttgacgctg ctgatcatcg gtgacacggg ggcggggaaa tcggagacgc
      721 ttgaagcgtt caggaccatg ggagaagaca ggatcgagga gatgaccatt atcgccgatg
      781 acatggggtc gctggacata ggacaggaag ggagcgtcat cggctacggt acggaggtcg
      841 gggcctttgt caggctcgat gacctccagc cgggctatgc cttcggtcag ctcgaccgct
      901 cgatcatcat gaaccccagc caggttaact cacggatcgt gctgccggtg accacgtacg
      961 agaacgtgat ggaaggctgt tccgttgaca tggtgctcta tgcgaacaac tacgaggaga
     1021 ccgacgagga gcacccgatc atcgagaggc ttaatacccc cgaaggcgcg ctgcacgtgt
     1081 tccgggaggg gaccgccatg agcaagggca ccaccacatc ggttggcctg gtgcacacat
     1141 atttcgtgaa tgtcttcgga gcggtccagt acaaggcgct tcacgacgac atcgccagga
     1201 ggttcttccg ggccttcttt gaaaaaggga tattcgtggg gcagttgcgg acgaggctgg
     1261 ggatccccgg ctgggaacgc aaaggaccgg aacaggccgc gcaggcgctg ctgggaatga
     1321 tcgcggatag atgaggtccg ggccttcccg gaccgggtgg tcgccggccg gcgggtcgtg
     1381 cctgtcccac ccgatggtcc gcccgtcctg gcgggcatca cctttttacc ccccactgcg
     1441 ttaaggcggt agccggagat catgcgaagg acaggaccgg gaggcacgaa ctccatcccg
     1501 ttatgggaag ggaggcctta tcctccccga cgcttggaag atgggcccgt tctttttctg
     1561 cggtgcctct gtgtcgacgg acccgccggt ttgggtccgg aggcacagag gccggcgcgc
     1621 gccgacccgg gggaacccgt gccgggtttt ttgttgtctt cctttcctgt ccccgcccgt
     1681 tccggcttca cctgcgccct gtcgcgtctt tgcggccgcg gcggccgcgc gaattcaacg
     1741 ttgcgctcag gcgcgggtgt cgtgtagtca aaatccttca ccgtgcgccg ctcgatcctg
     1801 gcaccgagca cacgttcgat ggccctgacc tggggctcgt cttcctgaca gacgaaggtg
     1861 aatgcgtccc ccgtcttagc cgcgcggccg gtgcggccga tgcggtgggt gtatgcgtcc
     1921 gtggtgtccg gca
//
LOCUS       DAOPOA010000503         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_64343_length_1925_cov_3.247594, whole genome shotgun sequence.
ACCESSION   DAOPOA010000503 DAOPOA010000000
VERSION     DAOPOA010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64343_length_1925_cov_3.247594"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PKJ17_09775"
     CDS             complement(<1..303)
                     /locus_tag="PKJ17_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76306.1"
                     /translation="MVDVFTYIIGVLCIVAVSGHIPFVYPLVLFGAMGLSVYLDLKKV
                     SIPRWILTVVSVAVILYFLIRIDVQDLIGQIMEALFILMGIKYLEHREVRDFMQIYA"
     gene            complement(394..1149)
                     /locus_tag="PKJ17_09780"
     CDS             complement(394..1149)
                     /locus_tag="PKJ17_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOD76307.1"
                     /translation="MSISGVFGKTNLSKLDLMIEFPQEVYAGSRVPVKITMRNRRRFL
                     PAFLIGLSADRISALLPFTPRKGTSSVYTSVTFHERGLHTIENPGVHSVFPFNFFTRF
                     KRLHRTYTITVLPRLKACDLASLYRKERRSRGEKTSDTIGYDSDIVSIREYVRGDPIK
                     YIHWKATAKTGKLKTKEHSSLSYQPVIIEFDRVLIRDIEERISCVAYTIVQLLRNSVP
                     VGLRIDGRLYPPDVTGVHKLNMLRELAVYEKHG"
     gene            complement(1266..>1925)
                     /locus_tag="PKJ17_09785"
     CDS             complement(1266..>1925)
                     /locus_tag="PKJ17_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOD76308.1"
                     /translation="EFHPGPIFNNIVLCDEINRATPKTQSALLEAMEEKQVTIEGKTY
                     KLPRLFCVIATQNPTEQFGTFPLPESQLDRFMMKISIGYPSRQAEKEILKIGSKREDI
                     YDIEPVVNQDEVSRIQEEISRNIHVSDKILEYTLSIIHETRTSEYLTAGLSTRGALTI
                     VNTARANAFFHDRHFVIPEDIRDLVEYTIPHRVIFKEEYENLNKREIVRSIVDAVPTP
                     L"
BASE COUNT          409 a          490 c          576 g          450 t
ORIGIN      
        1 ggcgtatatc tgcataaaat cacggacctc tctgtgttcc aggtacttta ttcccatgag
       61 aatgaaaagg gcctccatga tctgccctat caggtcctgc acgtcgatgc gaatgagaaa
      121 gtagaggatg acggccaccg aaacgacggt gaggatccag cggggaatgg agaccttttt
      181 caggtcgagg tagaccgaga ggcccatggc gccgaaaagc acgagaggat agacaaaggg
      241 aatgtggccg gacacggcaa ctatgcaaag caccccgatg atgtacgtga aaacatcaac
      301 cacggtgcgg actttcattt gtgtgaaacg gatcgtgatc atatatcttc agcgaacctc
      361 agtctgcccc cctcacctat aactcatcac ccatcaccca tgcttctcat acaccgccag
      421 ttcccgcagc atgtttagct tgtggacccc cgtcacgtcc ggggggtaga ggcgaccgtc
      481 tatcctgagc ccaacgggca cgctgttcct gaggagctgg acgatggtgt aggcgacgca
      541 ggagatgcgt tcctcgatgt ccctgatgag gaccctgtcg aattcgatga tgacgggctg
      601 ataggacagc gacgaatgct ccttcgtctt gagtttgcct gtcttggccg tcgccttcca
      661 gtggatgtac ttgatggggt ctccccggac atattccctg atggacacga tatcggaatc
      721 gtagcctatc gtgtcggacg tcttttcccc tctcgatctg cgctccttgc ggtacaggct
      781 tgccagatcg caggccttca gtctcggaag gaccgttatc gtataggtcc ggtgcagtct
      841 cttgaaccgg gtgaagaaat tgaaagggaa cacggagtgc acgccggggt tctctatggt
      901 gtgaaggccc cgttcatgaa aggtcacact ggtgtagacc gaagaggtcc ctttgcgcgg
      961 ggtgaagggc aagagggccg agatccggtc ggcgctcaag ccgatgagaa aggcgggaag
     1021 gaacctgcgg cggttccgca tcgttatctt cacggggacc cggcttcccg cgtacacctc
     1081 ctgggggaac tctatcatga ggtccagctt cgagagattt gtcttcccga agacccccga
     1141 tatgctcatg aagctcagga gcgccgaaac aacgagaaag atgaggttgt tgttggtgtt
     1201 cgccgccgcg atgccaagaa agatggtgag cgctatgtag atgaagcctg ccttggatat
     1261 ctttattaca ggggtgtggg aacggcgtct acgatcgatc ttacaatctc ccttttgttg
     1321 aggttctcat actcttcctt gaagatgacg cggtgaggga tggtgtactc gacgaggtcg
     1381 cggatatcct cgggtatgac gaagtgcctg tcgtggaaga aggcgttggc ccgcgcggta
     1441 ttgacgatcg tcagcgcacc ccgcgtggac agacctgccg tgaggtactc gctcgttctc
     1501 gtctcatgga tgatggacag ggtgtattcg aggatcttgt cagaaacatg aatgttgcga
     1561 gagatctcct cctggatgcg ggagacctcg tcctggttta cgacgggttc gatatcgtat
     1621 atgtcctctc tcttgctgcc gatcttgagg atctcctttt cggcctgccg cgacggatag
     1681 ccgatgctga tcttcatcat gaaacggtca agctgcgatt cggggagggg gaaggtgcca
     1741 aactgctccg tcgggttctg cgtggctatg acacagaaaa gccggggaag cttgtaggtc
     1801 ttgccctcga tggttacctg tttttcctcc atggcctcga ggagcgcgct ctgcgtcttt
     1861 ggggtggcac ggttgatctc gtcacagagg acaatattgt tgaagatggg accgggatgg
     1921 aactc
//
LOCUS       DAOPOA010000504         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_64516_length_1921_cov_3.466238, whole genome shotgun sequence.
ACCESSION   DAOPOA010000504 DAOPOA010000000
VERSION     DAOPOA010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64516_length_1921_cov_3.466238"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1776)
                     /locus_tag="PKJ17_09790"
     CDS             complement(217..1776)
                     /locus_tag="PKJ17_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76309.1"
                     /translation="MGFFSRLLGSKKDEDEAIRPPVRQEAARVTRGEPPRSAPSSISS
                     PEERKRIEQIVSQSLDDFSDAVGEMIETGNWGVGKVPSKTPATGYYTRFRDTPFTDEK
                     GNLRPEIAKGLAVAEKPWGGYDPAWNAVDAANRYYESFPQDIGEKEFTVDSGDDAMYV
                     KELQATLAYNTDNVDREVSKLLPKGPKNIKGDDEGIMWYINSDGFGSATSVPWIDDTV
                     NWLATVRNSAAGIKKDLEGIEDGVLRTMLLNYLDEQNDLVTRMYMRFSGCGQRVQRMR
                     EAFVMWNGVPKSENGTLCHELEDYKVVRQSLLHYGRMWYHGLFTAKGEEHARDADVDR
                     EYYAENDDFAGTSLSMRRVYAILSMCCCMKYALDNLRAYSAFAYPVVRDSMDRHLADI
                     NNYLSFRGYTDDGDPGKKYHEIFQKVKTHTFDRLNELGGAMLHYLKENGKIASGAARS
                     AMHVARAHESESQIRWGLTDALWRRMDLHEKLAISTPEFRYFVPWSAKPGELLEIGGI
                     RERQRDPGGAR"
BASE COUNT          345 a          585 c          514 g          477 t
ORIGIN      
        1 tgagctcgac gaggatgtcc atatctcggg tggcaaatgg ctctatgtag agtatggctg
       61 ccattcctcc tgctaatgcg taaccgtcta tcaggccttc ctgcacgatc tgattcaagg
      121 tttggattgt tcttttcagg gacatgggaa cctcaccggc tgtttgccaa acctttttgg
      181 tctatgaccg cgaacatctt ctcttcgaag acagcttcac cgcgcaccgc ccggatcccg
      241 ctgccgttcc cggatcccgc caatctccaa cagttcgccg ggctttgccg accaggggac
      301 gaaatacctg aactcggggg tggagatggc caatttctcg tgcagatcca tcctgcgcca
      361 gagggcgtcg gtgaggcccc accggatctg gctctccgac tcatgggcgc gggcaacatg
      421 catggcacta cgtgcggcac cggaggcgat cttgccgttt tccttgagat agtggagcat
      481 tgcccctcca agctcattga gcctgtcgaa ggtgtgcgtt tttacctttt ggaaaatctc
      541 atggtatttc ttccctgggt cgccatcatc cgtatagccc ctaaaggaga ggtagttatt
      601 gatgtcggcg aggtgcctgt ccatggagtc cctcaccacg gggtatgcga aggcagagta
      661 cgctctgagg ttgtcgaggg catacttcat gcagcaacac atcgaaagga tcgcgtagac
      721 gcgcctcatg ctcagcgacg tgccggcaaa atcgtcgttc tcggcgtagt attcccggtc
      781 gacatcggcg tcacgcgcgt gttcctcgcc cttggccgtg aacagaccgt gataccacat
      841 ccggccgtaa tgcaacaggc tttgtctcac aaccttgtag tcttcgagct catgacagag
      901 ggtgccgttc tcagacttcg gcacaccgtt ccacattaca aaggcctcgc gcatcctctg
      961 tacacgctgt ccgcaaccgg aaaagcgcat gtacatccgc gtcaccaggt cgttttgttc
     1021 gtcgaggtaa tttaagagca tcgtccgcaa gactccgtcc tcgatgcctt ccagatcttt
     1081 ctttatgcca gccgcagagt tgcgcaccgt ggcgagccaa ttgacggtgt catctatcca
     1141 tggcaccgac gtcgcagaac cgaacccgtc gctgtttata taccacataa taccttcgtc
     1201 atcgccctta atgttttttg gtcccttcgg gagcagcttg cttacctccc tgtcgacatt
     1261 gtcggtgtta tatgcgagcg tggcctgcaa ttctttcacg tacatggcgt cgtcgccgga
     1321 gtccacggta aactctttct cgcctatatc ctgggggaag ctctcgtagt acctgttcgc
     1381 ggcatccacc gcgttccagg cggggtcata cccgccccag ggcttctcgg caaccgcaag
     1441 gcctttcgcg atctccgggc gtagattacc cttttcatcc gtaaaggggg tgtcacgaaa
     1501 ccgcgtgtag tagcctgttg cgggggtctt tgacgggacc ttcccgacac cccagttgcc
     1561 cgtttctatc atctcgccga cggcatcgga gaagtcgtcg aggctctgtg acacgatctg
     1621 ttcgatcctt ttcctctctt ccgggctgga gatcgaagac ggcgcggacc gggggggctc
     1681 cccgcgggtc acccgcgcgg cctcctggcg gaccgggggc cttatggcct cgtcctcgtc
     1741 ctttttactc cccagcaacc ggctgaaaaa tcccatcctc cctcccgcgt ggcagctttt
     1801 gtcctttcct agacactatt ttctacagaa gtagcatctt tttgcaactt ttttacattc
     1861 cgcccgggcc tgagggcccc gtggtctccg ggttgtcgtc atcgggacgg ggtgggcggc
     1921 g
//
LOCUS       DAOPOA010000505         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_65245_length_1904_cov_3.633315, whole genome shotgun sequence.
ACCESSION   DAOPOA010000505 DAOPOA010000000
VERSION     DAOPOA010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65245_length_1904_cov_3.633315"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PKJ17_09795"
     CDS             <1..883
                     /locus_tag="PKJ17_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOD76310.1"
                     /translation="TRPCRYEPDLNPTYQDMAAHYGTAVIPARVKKPKDKAKVESAVL
                     AAERWIIAALRNHAFFSLAELNTAIREKLADYNGRELQKLKVSRKHLFETIDRPAMKA
                     LPLRRYEYAEWARHKVNIDYHVEIGRHYYSVPYQLRQQTVDARVTASTVEIFFRGKRV
                     ASHVRNPVPGRHTTVPAHMPEAHRRYLEWTPSRIISWAERTGPSTASLVKGIMERRPH
                     PEQGFRSSLGIIRLGRHYGQERLEAACTRALFMKAYSYRSVESILKNNLDGKELPGQV
                     NRPPVAHGNIRGGGYYT"
     gene            898..1653
                     /gene="istB"
                     /locus_tag="PKJ17_09800"
     CDS             898..1653
                     /gene="istB"
                     /locus_tag="PKJ17_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOD76311.1"
                     /translation="MLTEQTFEKLYTLKLFGFAKALKEQIEQPEMNDLTFEERIAMLA
                     DAEYLFRENARMKRLLKNAKLKLAASLEDIDYRSPRGLDKSVIRSLGTCGWIRKHQNV
                     IIVGPTGTGKTFLACALAQMACREGISAFYLRTPALYRLLVIGRADGSYARVLTRLAR
                     ISLLVLDDLGLAALADSERRDLLEVIEDRHGAGSTIITSQLPIDHWHEVIGDPTIADA
                     LLDRLVHNAHKINLKGESMRKRKAALTQQEERV"
BASE COUNT          462 a          540 c          556 g          346 t
ORIGIN      
        1 gacccgcccg tgccggtatg aaccggatct caatccgacc taccaggaca tggcggcgca
       61 ctacggcacg gcggtcatcc ctgcacgggt aaagaaaccg aaggacaagg caaaggtcga
      121 atcggcggtg ctcgcagccg agcggtggat catcgccgcg ctgcgcaacc atgccttttt
      181 cagcctcgcg gaactcaaca ccgcgatcag ggagaaactc gctgattata acggcaggga
      241 actgcagaaa ctgaaggtgt cgagaaaaca cctcttcgag acgatcgaca ggcccgccat
      301 gaaggccctg cccttaagac ggtacgagta tgccgagtgg gcaaggcaca aggtgaacat
      361 cgactaccat gtcgagatag gccgccatta ctacagcgtg ccgtaccagt tgaggcagca
      421 gactgtcgac gcccgggtca cggcatcgac cgttgagata ttcttcaggg gaaagagggt
      481 cgcctcccac gtaaggaacc ctgtccccgg cagacacacg acagtccctg cccacatgcc
      541 cgaggcgcac aggcgctacc ttgaatggac accgtcacgc atcatcagct gggccgagag
      601 aacagggcca tccacggcct cgctcgttaa ggggatcatg gagcgaaggc cccatcccga
      661 gcagggcttc aggtcctccc tcggcattat acgcctcggc aggcactacg ggcaggagag
      721 gctggaggcc gcatgcacgc gggcgctctt catgaaggcg tattcataca ggagcgttga
      781 atccatcctg aagaataatc tggacgggaa ggagcttccc ggacaggtga accgtccccc
      841 tgttgcccac ggcaacatcc ggggtggcgg ctattacacg tgacaaggag gaggactgtg
      901 ctgaccgaac agacgtttga gaaactttac accttaaagc tctttggctt cgctaaggcg
      961 ctcaaagagc agattgagca gccggagatg aacgacctta cttttgaaga gcgcatcgcc
     1021 atgcttgccg atgctgaata tctcttcagg gagaacgcga ggatgaagcg tctcctgaag
     1081 aatgcgaaac tgaagctcgc cgcctcattg gaggacatcg attaccgctc cccccgcgga
     1141 ctcgacaaat cggtgatccg gagcctgggc acctgcggct ggatacggaa gcaccagaat
     1201 gtcatcatcg tggggccgac cggaacggga aagacgttcc tcgcatgcgc ccttgcccag
     1261 atggcctgcc gggaagggat aagcgctttt tacctgagaa cgccggcgct ttaccgtctg
     1321 cttgtcatcg gcagggcgga tgggagctac gcccgggttc tcacgcggct ggccaggata
     1381 tccctcctcg tcctcgatga tctggggctt gcagcactgg ctgacagcga gcgcagggat
     1441 ttgctcgagg tcatcgagga ccgccatgga gcgggctcga ccatcatcac aagccagttg
     1501 cccatagacc actggcatga ggtcatagga gacccgacca ttgccgatgc ccttcttgac
     1561 cgactcgtgc ataacgccca taagatcaac ctgaaaggcg aatcgatgag gaagagaaag
     1621 gcggcgttga cacaacagga ggaaagggtg taatgtagat caacttccag gcgtcgcctg
     1681 acggctttcg accggggtgg ccacattcat cataatgggt ggccaatttg atccggaaca
     1741 ggcggccacg ttcgatcata ccgggcggct aatttaaacg gaatacgcaa taggtataac
     1801 aaggggggga cttgtggtaa attaccacaa gtccccccct tgtacgttac aagcttatga
     1861 ttccattaag gattctggtc aaaaaccgta tggaattcac aatc
//
LOCUS       DAOPOA010000506         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_65287_length_1903_cov_4.446429, whole genome shotgun sequence.
ACCESSION   DAOPOA010000506 DAOPOA010000000
VERSION     DAOPOA010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65287_length_1903_cov_4.446429"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKJ17_09805"
     CDS             <1..281
                     /locus_tag="PKJ17_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76312.1"
                     /translation="AHYMGFIAPDDFGILTSINLLVMLFLGGIGTIFGPALGALFLKL
                     LPEITYQFQDYELLINGIILIVVLVFFPRGLFGIFTALRNKIVPRMGK"
     gene            278..1081
                     /locus_tag="PKJ17_09810"
     CDS             278..1081
                     /locus_tag="PKJ17_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD76313.1"
                     /translation="MMEKNGHILTISGVTKTFDGVRALDDVSFSVRSGDIKALIGPNG
                     AGKTTLLNAVSGLNPPTKGSVLFEGRDVVGLRTDQIASLGMSRTFQLIRLFTINNATV
                     LDNVLLGAHMHLRPTLLETVFLRGRIKRQEREATAKAMDLLKLVGIEKEAHALPGELS
                     FGSQRLLELARALMTGPRMLLLDEPASGLNDAEVEEFTRLLMTIKQQGITILIVEHNM
                     KLVMNIADDIIVLDFGRKLAEGPPSAICANPDVIEAYLGAQSKECGVTQ"
     gene            1078..1788
                     /locus_tag="PKJ17_09815"
     CDS             1078..1788
                     /locus_tag="PKJ17_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD76314.1"
                     /translation="MILKAERLSAGYARIQVLKAIDLEVKKGEIVCLVGANGAGKSTL
                     LKVLSGVIPPTAGAFTFDGRDVTGRKPNQLVRMGLSHVPEGRQIFGSLTVKQNLFLGA
                     YVNPAKKERLDDLLESVFGLFPKLKERLAQKAGTMSGGEQQMLAIGRGLMSQPKLLLL
                     DEPSLGLAPLVVEAILSTIGELRSRGISILLVEQNVNAALHISDRAYVLETGRIVLQG
                     EAKDLLENEEVKRCYLGM"
BASE COUNT          426 a          586 c          537 g          354 t
ORIGIN      
        1 tcgcccacta catgggcttc atcgcccccg acgacttcgg catactgacc tcgataaatc
       61 tccttgtcat gctctttctc ggaggcatcg ggacgatatt cggccccgcc ctgggggccc
      121 tcttcctcaa gctgctgccc gagatcacct atcaattcca ggattacgag ctcctcatca
      181 acgggatcat ccttatcgtg gtgctcgtgt tcttccccag gggcctcttt ggaatattca
      241 cggccctcag gaacaagatc gttccacgga tgggcaaatg atggaaaaga acggacacat
      301 actgactatc agcggcgtga ccaagacctt cgacggggtc agggccctcg acgacgtgag
      361 cttcagcgtc agatcgggcg acatcaaggc gctcatcggc cccaacggag ccggcaagac
      421 aaccctcctc aatgccgtca gcgggctcaa ccccccgaca aagggctccg tcctttttga
      481 aggccgtgat gtcgtcgggt taaggaccga ccagatcgct tcgctgggga tgtcccgcac
      541 gttccaactc atccggctct tcacgatcaa caacgccacc gttctcgaca acgtccttct
      601 gggggcccac atgcacttga ggccgacgct cctcgagacg gtcttcctga gaggaaggat
      661 aaaaaggcag gaaagggagg ccacagctaa ggccatggac ctcctgaaac tcgtcggcat
      721 cgaaaaagag gcccacgcgc tccccgggga gttgtccttc ggcagccagc gtctcctgga
      781 acttgcccgg gcgctgatga ctggccccag gatgctcctt ctcgatgagc ccgcgtcggg
      841 gctcaacgac gccgaggtgg aggagttcac gcggctcctc atgaccatca agcaacaggg
      901 tatcaccatc ctcatagtag aacacaacat gaaactggtc atgaatatcg ccgacgacat
      961 catcgtcctc gatttcggaa ggaaactggc ggaagggccg ccctcggcca tctgtgctaa
     1021 ccccgacgtc attgaggcgt atctgggcgc gcagagcaaa gagtgcgggg tgacgcaatg
     1081 atcctcaagg ccgagagact atcagccgga tatgcccgta tacaggtcct caaggcaata
     1141 gacctggagg tgaagaaggg tgagatcgtc tgtctcgtgg gggccaacgg ggctgggaaa
     1201 tcgacccttc tcaaggtcct gtccggcgtg ataccaccca cggcgggcgc gttcaccttc
     1261 gacgggcggg acgtgaccgg acgcaagccc aaccagctcg tgaggatggg gctcagccat
     1321 gtccccgagg gacgccagat cttcggctcc cttacggtca aacagaacct tttcctcggt
     1381 gcctacgtca accccgcaaa gaaagagcgc ctcgacgacc ttctcgaatc cgtcttcggc
     1441 ctcttcccaa agctcaagga acgcctcgcc cagaaggcgg gaacgatgag cggcggagag
     1501 cagcagatgc ttgccattgg cagggggctc atgtcccagc cgaagctcct gctgctcgac
     1561 gaaccttccc tggggctggc tccgctcgtc gtggaggcca tactgagcac catcggagag
     1621 cttcgcagcc ggggcatctc catcctcctc gtcgaacaga atgtgaacgc cgcccttcac
     1681 atatcggacc gcgcctacgt gctagagacg ggcaggatcg tgctgcaggg agaagcaaag
     1741 gatcttttgg agaacgagga agtaaagaga tgttatctgg gcatgtaaga cacagcaaat
     1801 ttcacgggag gtaatcaact catgcttata ggcgatctac ccactcaatg cgcgcgccgg
     1861 tatcccgaca agacggctct ggtctccgaa gaaaacaggc tca
//
LOCUS       DAOPOA010000507         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_65378_length_1901_cov_4.438245, whole genome shotgun sequence.
ACCESSION   DAOPOA010000507 DAOPOA010000000
VERSION     DAOPOA010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65378_length_1901_cov_4.438245"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="pheS"
                     /locus_tag="PKJ17_09820"
     CDS             complement(<1..925)
                     /gene="pheS"
                     /locus_tag="PKJ17_09820"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOD76315.1"
                     /translation="MDISGLKEKIEGEISRIGNIEDLKQVKVSLLGRKGVVADYLDKL
                     KTLDKDQRRDAGKAINEIKTWADLKIGELEKHFNEIERQNRERASLIDITMPGTAPVF
                     GKKHPITLTLEEIIRIFGSLGFAVAEGPDIEAEYYNFEALNIPSDHPARDMQDTFYIR
                     PGVVLRTHTSPVQIRTMEAQLPPVKIIAPGCVYRCDNDISHTPMFHQVEGLMVDKNVR
                     FSDLKGILSIFIHEMFGPDTPLRFRPSYFPFTEPSAEVDIGCVICRGAGCRVCKDTGW
                     LEILGSGMVHPQVFRNVGYDQEEITGFAFGMG"
     gene            complement(934..1293)
                     /gene="rplT"
                     /locus_tag="PKJ17_09825"
     CDS             complement(934..1293)
                     /gene="rplT"
                     /locus_tag="PKJ17_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOD76316.1"
                     /translation="MPRAKRGFKARQRRKKIFKLAKGMTQRRKNVYSVAKRSVFKALK
                     YAYIGRKERKRDFRSLWIVRINAACRSYGIPYNRFINGLKVANIALNRKSLADMAVND
                     PAGFGEVVAKVKAVVTH"
     gene            complement(1319..1516)
                     /gene="rpmI"
                     /locus_tag="PKJ17_09830"
     CDS             complement(1319..1516)
                     /gene="rpmI"
                     /locus_tag="PKJ17_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOD76317.1"
                     /translation="MPKIKTHRGLAKRVKVTAGGKIKRAKAFHSHLLSSKTPKMKRRL
                     SKADTVHPADAKRIKSLIPYS"
     gene            complement(1535..>1901)
                     /gene="infC"
                     /locus_tag="PKJ17_09835"
     CDS             complement(1535..>1901)
                     /gene="infC"
                     /locus_tag="PKJ17_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOD76318.1"
                     /translation="VAGKSSPPVCKIMDYGKYKYQLSKKAQEAKKKQSVIQIKEMKLG
                     LKIEEHDFQFKMKHLRGFLEEGHKLKIIIMFRGREILHVDMGEKLAQKVVDSLKDVGE
                     LEQRSRFDGRNIVMIFAPL"
BASE COUNT          329 a          549 c          509 g          514 t
ORIGIN      
        1 cccccatgcc gaaggcgaag cctgtgatct cctcctggtc gtaaccgaca ttgcggaaga
       61 cctgggggtg gaccatgcct gagcctagta tctcgagcca gcccgtgtcc ttgcataccc
      121 ggcagcccgc gccccggcat atcacgcagc ctatgtccac ttcggcggag ggctccgtga
      181 agggaaaata gctgggccga aatctcaagg gtgtgtcggg cccgaacatc tcgtggatga
      241 atatgctcag gatccccttg agatcggaga aacggacgtt cttgtccacc atcaatccct
      301 cgacctgatg gaacatcggc gtgtgggaga tgtcgttgtc gcagcggtaa acgcagccgg
      361 gggcgatgat cttgaccggc ggcagctgcg cctccatggt gcgtatctgc accggcgagg
      421 tgtgggtcct caggacgaca ccgggtctta tgtagaacgt gtcctgcatg tcccgggccg
      481 gatggtccga ggggatattg agggcctcga aattgtagta ttccgcctcg atatccgggc
      541 cttcagccac ggcgaaaccg agggatccga agatccttat gatctcttcg agcgtgagcg
      601 tgatagggtg cttcttcccg aaaaccgggg cggtgccggg cattgtgata tcgatgagcg
      661 aggcccgttc cctgttttgc ctttcaatct cgttgaagtg cttttccagt tcgccgatct
      721 tcaggtccgc ccacgtcttg atctcgttga tggccttgcc ggcatcgcgc cgctggtcct
      781 tgtcgagggt tttgagctta tcgaggtagt cggcgacaac cccttttctc ccgagaaggg
      841 ataccttcac ctgcttgagg tcctcgatgt tcccgatacg ggatatttcg ccttctatct
      901 tctctttcag tccgcttatg tccaagcgtc accctagtgc gtgaccactg ccttgacctt
      961 tgccaccact tcgccgaatc ccgcggggtc attgacggcc atgtcggcga gcgatttccg
     1021 gttgagggcg atgttcgcga ccttcaaccc gttgatgaag cggttatagg gtatgccata
     1081 ggaacggcag gccgcgttga tgcggacgat ccacagggac ctgaagtccc gtttccgttc
     1141 cttgcgcccg atgtaggcgt atttgagggc cttgaagacg ctcctttttg ccacgctgta
     1201 cacgtttttc ctgcgctgcg tcatgccctt cgcaagcttg aatatcttct ttcttctctg
     1261 tcttgccttg aatcctcgtt ttgcccttgg catggtcttg tacctctctt gaacctttct
     1321 atgaatacgg tatcagtgac ttgatcctct ttgcgtccgc cggatggacg gtgtcggcct
     1381 ttgagagccg tcttttcatc ttcggtgttt tcgaagacaa cagatggctg tggaaggcct
     1441 tggcgcgttt tatcttgccg ccggcggtga ccttcacgcg cttcgccaac ccgcgatggg
     1501 tttttatctt tggcatctga acctccttaa gtgatcacag gggggcaaag atcatcacga
     1561 tgttcctgcc gtcaaatctg gacctttgct ccagttcccc cacgtctttc aatgaatcta
     1621 caaccttttg cgccagcttc tcacccatgt caacgtggag gatctcccgt cccctgaaca
     1681 tgatgatgat cttcaacttg tggccctctt cgagaaaccc cctgaggtgt ttcatcttga
     1741 actggaagtc gtgttcctcg atcttgagcc ccagtttcat ctccttgatc tggatcacgc
     1801 tctgtttctt cttcgcttcc tgggcctttt ttgaaagctg atacttgtat ttcccgtaat
     1861 ccatgatctt gcagaccggc ggggaggatt tccccgccac c
//
LOCUS       DAOPOA010000508         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_65495_length_1899_cov_3.236985, whole genome shotgun sequence.
ACCESSION   DAOPOA010000508 DAOPOA010000000
VERSION     DAOPOA010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65495_length_1899_cov_3.236985"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..1677
                     /locus_tag="PKJ17_09840"
     CDS             364..1677
                     /locus_tag="PKJ17_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOD76319.1"
                     /translation="MKNDDMAEVDLAKGQRTALFASAVTFGLAAVKALIGYGFHSPLL
                     VADGFHTFSDFTVIFASWFGLFLAARKKTERFPYGLYKAETLVSFIIGLLVTWAAIEV
                     LLEGVRKFGPPASTGAFPVLPVTVSCVSMAIGYFLARQEGSVGNEINSQSLKTIAQDS
                     YLNIAVSFAVLAGILAAWFSIPYVEGVLLVIISLLVLKLGLETIWFSLLILLDANLDR
                     PLQSDIIREISDIQGVTGVRNVKIRQAGPFRLVECNIESSPNAPLYQAHGVADRVEEH
                     IKANFPYIESVYVHVEPGKSKTTVAIIPVRDVDGLNSRVYDHFGRAPYFIVVKIGGNG
                     VEIEDFYYNEHLGKKGKIHVGIKVIRELTRYGLNTLFTSQIGEISFHMLKDAFVDIYK
                     AREDESVAEVIERFRHGDIEVLKAPTHTAEKSLAEGVIRTEGNES"
BASE COUNT          391 a          565 c          555 g          388 t
ORIGIN      
        1 ggtccatgga ggaatggtgc gcggacgcct cggcgcctct cgtcgaggac cggtaagcag
       61 gacacgggca gtttgctgat gcgctgagag agcatatatc gcaccgtcag aagcggaggt
      121 cagcagtgtc attcggattc agaggcaggc acggccatcg caggcacggc ggaaggaccg
      181 tcaccggcca aaccggagac cagggccgcg cggctaccat tccaccacgg cagtgcatct
      241 gcccgcattg cggcatgata acggacaagg tcccggggca accctgtttc ctgacgccct
      301 gcccccgttg tcagggcatg atggcgggac ggttcccggc ggaggaacga cgggaacaaa
      361 gtcatgaaga atgacgatat ggccgaggtc gatctggcaa agggtcaacg aacggccctt
      421 ttcgcctctg ccgtgacctt cggcctcgcc gccgtcaagg cccttatcgg ctacgggttc
      481 cattctcccc ttctcgtcgc tgacggcttc cacacctttt ccgatttcac ggtgatattc
      541 gcctcgtggt tcgggctttt cctggcagcc cgcaagaaga cggagcgttt cccttatggc
      601 ctctacaagg ccgaaaccct cgtcagtttc atcatcggac tcctcgtgac ctgggcagcg
      661 atcgaggtcc tcctcgaggg ggtcaggaag ttcggtcccc ccgcatcgac aggcgccttc
      721 cccgtgttgc cggtcaccgt ctcttgcgtc tccatggcca tcgggtattt cctggcgcgc
      781 caggaaggct ccgtcgggaa tgagatcaat tcccagtcgc tcaaaacgat agcccaggat
      841 tcctatctca atattgccgt gtccttcgcg gtcctcgcgg gtatcctggc cgcctggttt
      901 tccatcccct atgttgaggg agtgctcctc gtcatcatct ccctgctcgt cctgaagctg
      961 ggtctcgaga ccatctggtt ttccctgctc atcctcctcg atgcgaacct cgaccgtccg
     1021 ctgcagtcgg atatcatacg ggagatcagt gacatacagg gtgtgaccgg ggttcggaac
     1081 gtgaagatac gacaggcggg gcccttcaga ctggtcgagt gcaatatcga aagcagcccc
     1141 aacgcgcctc tctaccaggc acatggcgtg gccgacaggg tggaggaaca cattaaggcg
     1201 aacttccctt acatcgagtc cgtgtacgtc cacgttgagc ccggaaaaag caagaccacc
     1261 gttgccatta tccccgtgag ggacgtcgat ggcctcaatt caagggtcta tgaccatttc
     1321 ggcagggcac cctatttcat cgtcgtgaag atcgggggga acggagttga gatcgaggat
     1381 ttctattaca acgagcacct cggcaaaaaa ggcaaaatcc acgtagggat caaggtgatc
     1441 agagaactga ccaggtacgg gctgaacacc cttttcacct ctcagatagg ggagatatcc
     1501 tttcacatgc tcaaggatgc cttcgttgac atatacaagg cccgcgagga tgaatcggtt
     1561 gcggaggtca tcgaacgctt tcgccatggg gacatcgagg tcttgaaagc tcccactcat
     1621 accgccgaga agtcgctggc cgaaggcgtg atacgaacgg aagggaacga gtcatgacgg
     1681 gtccggcgcc gtcgagaaga tggtggaacc gggttatccc gtcgtcacgg gcatcggccg
     1741 gtacggatcc cccaacagga tgtgtctggg gaacgcagac gtggcggtct tctgaaaata
     1801 gtaccggggc cgggaatcac gcacggtttc ccggccccgg tactttgatc gcctaatctt
     1861 cgaacgccat ctgcggctgc cagaccgtcc agaccttcc
//
LOCUS       DAOPOA010000509         1895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_65697_length_1895_cov_2.815217, whole genome shotgun sequence.
ACCESSION   DAOPOA010000509 DAOPOA010000000
VERSION     DAOPOA010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65697_length_1895_cov_2.815217"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PKJ17_09845"
     CDS             complement(<1..1117)
                     /locus_tag="PKJ17_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76320.1"
                     /translation="MKVAAAYPGTPSSEILKDVADTYPEIYAEWSPNEMVALQVASGA
                     ALAGARAMASMKHVGMNVAADSLMTLAYTGIKGGLMIVVADDPNVHSSQNEQDSRNWA
                     RFGKVPMLEPGDAQECLDFTKAAFDISEKFDTPVLLRTVTRIAHADSLVQTGKRVESK
                     MPLGLDPKEAPKYVMVPAHVRPRRKAVEERMKKLEAFADKTKINKMEINSKSVGIIAS
                     GSAYMYAKEVFPEYSFLKLGMVWPLPKKMIADFFKQVKKVMIIEELDPFFETEIRAMG
                     YKVFHGKDIIPNMYELSPEIIEQAVKGKAYKAPKVRVKPEDLPRRPPNLCAGCSHRPL
                     FYALKKLGAFVFGDIGCYTLAVAPPISALHTTICMGAG"
     gene            complement(1325..>1895)
                     /locus_tag="PKJ17_09850"
     CDS             complement(1325..>1895)
                     /locus_tag="PKJ17_09850"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HOD76321.1"
                     /translation="GGAKFPGVKEALKTPKWRRLLEGNEIYNAILRNTVTLTILKGGE
                     KVNVIPAESSATFDARLLPAETHERFFRKVRRLAGKDVVIERIGEKISEPGPSRYNTV
                     YFQGIRNCVTSLKGQVPVLPFVTTGATDLRYFRDIGVPAYGFFPITLTKEEHMRMHGK
                     DERISLENIREGLEGCRGIVNFLASPSVP"
BASE COUNT          343 a          539 c          527 g          486 t
ORIGIN      
        1 tgcccgcgcc catacagatg gtcgtgtgaa gggcggagat cggcggggcg actgccagcg
       61 tgtagcagcc gatgtcgccg aagacaaagg cgcccagttt cttcagggcg taaaagagag
      121 gcctgtgcga gcagccggca cagaggttcg gcggccttct cggcaggtct tccggtttca
      181 ccctgacctt cggggccttg taggctttgc ccttcacggc ctgctcgatg atctccggtg
      241 agagttcgta catgttgggg atgatgtcct tgccgtggaa gaccttgtag cccatcgccc
      301 tgatctctgt ttcaaagaag gggtcgagtt cttcgatgat catgaccttc ttcacctgct
      361 tgaagaaatc ggcgatcatc ttcttcggaa gcggccacac catgccgagc ttcaggaagg
      421 agtattccgg gaagacttcc ttggcataca tgtaggcgga accactggcg atgatgccga
      481 cgctcttgga gttgatctcc atcttgttga tcttcgtctt gtcggcgaag gcttccagct
      541 tcttcatgcg ctcctcgacg gcctttcttc tcggccggac gtgggcgggg accatgacgt
      601 acttcggcgc ctctttcggg tcgagtccga gaggcatctt ggattcgact ctcttgcccg
      661 tctgaacgag tgaatcggcg tgagcgatcc ttgtcaccgt tctcaggaga acgggggtgt
      721 cgaacttctc gctgatgtcg aaggcggcct tcgtgaaatc aaggcattcc tgggcgtcgc
      781 ccggttccag catgggtacc ttgccgaaac gggcccagtt gcggctgtcc tgctcgttct
      841 gggaactgtg gacgttggga tcgtcggcca cgacgatcat gagaccgccc ttgatacccg
      901 tgtaggcaag ggtcatcagg gaatctgccg cgacgttcat gccgacgtgc ttcatggatg
      961 ccatcgctct cgcgccggca agggccgcac ccgaggctac ctggagcgcc accatctcgt
     1021 tcggtgacca ttcagcgtag atctccgggt aagtgtcggc aacgtctttg aggatctcac
     1081 tgctcggtgt tccgggatat gcggcagcga ctttcacacc agcttcccag gcacctcttg
     1141 caattgctgc gtttccttgc attatttctt tcatactacc cccataatgg cgtatcgcct
     1201 ggattttgag tgactaattg cttcacctcc tttggaacga tttttcttgt attttaacta
     1261 aggcttgtta atgaattcac gtattatttt cgacagatta aacgtccttt gtcaagaaat
     1321 atcgttacgg aacactggga gacgccagga aatttacgat gcccctgcac ccttccaatc
     1381 cttccctaat attctcaagg gatatcctct catccttgcc gtgcatcctc atgtgttctt
     1441 ccttcgtgag ggtgatgggg aagaacccgt aggccggaac tccgatgtcc ctgaagtatc
     1501 tgaggtccgt cgcgcccgtc gtgacaaagg gaaggacggg gacctgtcct ttcaatgaag
     1561 tcacgcaatt ccttatgcct tgaaaataga ccgtgttgta ccgtgagggc ccgggttcgc
     1621 tgatcttctc tccgatacgc tctatgacga cgtcctttcc cgcgagccgc ctcaccttcc
     1681 tgaagaacct ctcgtgggtc tccgcgggaa ggagcctggc gtcgaaggtc gccgaggact
     1741 cggccggtat gacgttgacc ttttcgcctc ccttaagtat ggtgagcgtc accgtgttcc
     1801 tgaggatggc gttgtatatc tcattgccct cgaggaggcg cctccacttc ggtgtcttca
     1861 gggcctcctt cacgccgggg aacttcgctc cccct
//
LOCUS       DAOPOA010000510         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_66190_length_1885_cov_4.291257, whole genome shotgun sequence.
ACCESSION   DAOPOA010000510 DAOPOA010000000
VERSION     DAOPOA010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66190_length_1885_cov_4.291257"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PKJ17_09855"
     CDS             complement(<1..852)
                     /locus_tag="PKJ17_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOD76322.1"
                     /translation="MSGFTQIIQKDLDELESSIDELISTRVSYIKEIVTYIIRSGGKR
                     VRPVLVMLCSKLCGYRGRKHIPYAAIIEFIHTATLLHDDVVDNAKTRRGLSTVNTVWG
                     NEPSVLVGDFLYSRSFELMSRDGNNEILKTISKVTTALSEGEILEIVKTADVETTEKD
                     YYEIIGNKTAVLFGAACEIGAILGGRPDSERRALRNFGYDLGIAFQLMDDVLDYTSYN
                     DVLGKHVGTDLKEGKVTLPLIHVLRNAGEKDKTQIEKIFAKQKITQRDFDRVLAIIER
                     NGGIAYTL"
     gene            complement(855..1595)
                     /locus_tag="PKJ17_09860"
     CDS             complement(855..1595)
                     /locus_tag="PKJ17_09860"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOD76323.1"
                     /translation="MRDIVGKDETLDILCNEELRIIQKRKGYRFSIDAILLAAFVVLK
                     KHERLLDIGSGCGIIPIYISKKGCRNEMIGVELQTDLYETAQKNSLINHCEDHVRFIN
                     ADINTLLRDMRRTPFHVVLSNPPYTKRRSGRECPERSRFLARNEETLDLEALVGAASS
                     LLVKKGRFYVIYPARRAGELIQSAASRKLALKRLRTVHPRRDESANLILAEFVKDGGV
                     GAAIERPLYVYDGDAISAEVKEYYSFGD"
     gene            complement(1588..>1885)
                     /locus_tag="PKJ17_09865"
     CDS             complement(1588..>1885)
                     /locus_tag="PKJ17_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76324.1"
                     /translation="LYSSPDIPDGLKRLKVNAIHSGVIRGIMGIVDEEISFTEDHRWS
                     VDSVRNASSDLAFFLPPTTVDEVRDIAENGLDMPPKSTFFYPKILTGLVFYRYA"
BASE COUNT          367 a          517 c          535 g          466 t
ORIGIN      
        1 cagggtgtag gcgatgccgc cgttcctttc tatgatggca agcacgcgat cgaaatcacg
       61 ctgtgttatt ttctgcttgg caaagatctt ttctatctgg gtcttgtcct tttcaccggc
      121 attcctgaga acgtggatga gaggaagggt taccttgcct tccttcaggt cggttcccac
      181 gtgcttaccc agaacgtcgt tgtacgatgt gtagtcgagg acgtcatcca tgagttgaaa
      241 ggctatgccg agatcgtatc cgaaattcct caacgcccgt cgctcactgt cgggcctccc
      301 gccgaggatg gctcctatct cgcaggcggc tccgaaaaga acggccgtct tgttgccgat
      361 tatctcgtag taatcctttt cggtcgtttc cacgtcggcg gtcttgacta tctcgaggat
      421 ctctccctcg gacagtgccg tcgtcacctt cgagatcgtt ttgaggatct cgttattgcc
      481 gtcccgggac atgagctcga aagacctgga gtagaggaag tccccgacca gcacgctggg
      541 ctcgttgccc cagaccgtgt tcaccgtgga caggccccgc ctggtcttgg cgttgtccac
      601 gacgtcatcg tgaagcaggg tggcggtgtg gatgaactcg atgatcgcgg cgtagggaat
      661 gtgtttcctc ccccggtagc cgcacagctt ggagcagagc atgacaagga cagggcgcac
      721 cctcttgccg ccggatctta tgatgtaggt gacgatctct ttgatgtagc tgacccgagt
      781 gctgatgagc tcatcgatcg acgactcgag ctcatcgagg tccttctgga tgatctgcgt
      841 gaatcccgac atgactagtc tccaaatgaa tagtattctt ttacttccgc agagatggcg
      901 tctccgtcat acacatagag cggtctctcg atggcggccc cgactccgcc gtccttcacg
      961 aattccgcca ggatgaggtt ggcgctctca tctctccggg ggtggacggt gcgcagtctc
     1021 ttgagcgcca atttccgcga cgccgccgac tggatgagct ccccggcgcg acgggccggg
     1081 tagatgacat agaagcgacc ttttttcacg aggagagacg atgctgcgcc cacaagggcc
     1141 tcgaggtcga gggtttcctc gttgcgggcc aggaagcgcg acctttccgg acactccctg
     1201 ccgcttctgc gtttcgtgta cggggggttg gataggacca cgtgaaaggg tgtccgcctc
     1261 atgtcccgaa gaagggtgtt gatatcggcg ttgatgaaac ggacgtggtc ctcgcaatgg
     1321 tttatcaagc tgttcttttg cgccgtctca tagagatccg tctgtagttc cacgccgatc
     1381 atctcgtttc tacaaccttt tttcgaaata tagataggta tgataccaca accggaaccg
     1441 atatccaaca gcctttcatg ctttttcagg accacgaagg cggcgaggag gatcgcgtcg
     1501 atggagaacc ggtacccctt tcttttctgg atgatccgca gttcttcgtt gcagagaatg
     1561 tcgagggtct cgtcctttcc gacgatgtca cgcatacctg taaaatacaa ggcccgtcag
     1621 gatcttgggg tagaagaacg tcgatttcgg gggcatgtcg aggccgttct cggcaatgtc
     1681 tctcacctca tcgacggtcg tgggggggag aaagaatgcc agatcactgg aggcgttcct
     1741 cacggaatcg acagaccacc tgtggtcctc cgtgaaggag atctcttcgt caactattcc
     1801 catgatcccg cggatgaccc cactgtggat ggcattcacc ttcaatctct taaggccgtc
     1861 agggatgtcc ggagagctgt atagg
//
LOCUS       DAOPOA010000511         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_66500_length_1879_cov_3.186952, whole genome shotgun sequence.
ACCESSION   DAOPOA010000511 DAOPOA010000000
VERSION     DAOPOA010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66500_length_1879_cov_3.186952"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PKJ17_09870"
     CDS             <1..960
                     /locus_tag="PKJ17_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOD76325.1"
                     /translation="RLTLILKIKDLVEAMDYLRSAFEKKSKEFSQVIKMGRTQLQDAV
                     PMTLGQEFGAWAIMLAEDMDRMREAQALVKEINLGATAIGTGLNAHPDYSSLVAEKLR
                     QITGIDVLTSPDLVEATQDSGAYVQLSGVLKRFAVKISKICNDLRLLASGPRCGLNEI
                     NLPPMAPGSTIMPGKVNPIIPEVVNQVAFFVIGNDVTVSFAAEAGQLELNVMEPVMAF
                     SLFNSLVALRRASKTLADRCVLGITANQDKCREYVECSIGLVTALNPYIGYERSTEIA
                     HEALATNRSVCELVLEKGYLKKEELDDILSPENMIKPRYMEKK"
     gene            complement(1040..1459)
                     /locus_tag="PKJ17_09875"
     CDS             complement(1040..1459)
                     /locus_tag="PKJ17_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOD76326.1"
                     /translation="MKRFDVDNLSSLVIRAAINVHKVFGPGLLESIYQSSMVIELGLM
                     GIEVLTEVPLHVSYKGRKIPNVGLRLDLLVEDTIIVELKSVEEIQPVHKKQLLTYLRL
                     AGKPLGLLINFNTTLLREGITRVISSPQGDGNAARFR"
BASE COUNT          411 a          531 c          538 g          399 t
ORIGIN      
        1 cgactgaccc ttatcctgaa gatcaaggac ctcgtggagg cgatggacta tctgcgctcg
       61 gccttcgaga agaaatcgaa agagttttcc caggtcatca agatgggcag gacgcagctt
      121 caggatgccg tgccaatgac gctgggccag gaattcggcg cctgggccat catgcttgcc
      181 gaggacatgg accggatgag ggaggcgcag gcgctcgtga aggagataaa tctgggagcg
      241 accgccatcg gaacggggct caacgcccat cctgattatt cttcccttgt cgcggaaaag
      301 ctccgccaga tcacggggat cgatgtgctc acatcacccg acctggtgga ggcgacacag
      361 gattcgggcg cctatgtcca gctgtcgggc gtcctgaagc gcttcgccgt caagatctcc
      421 aagatctgca acgacctgag gctcctggcg tccggtccgc gctgcggcct caatgagata
      481 aacctccctc ccatggcccc cggctcgacg atcatgcccg gaaaggtgaa ccccatcatt
      541 cccgaggtgg tgaaccaggt ggcattcttt gtcatcggca acgacgtgac ggtgagcttc
      601 gccgcggagg cggggcagct ggagctgaac gtcatggaac ccgtcatggc cttcagtctc
      661 ttcaattccc tcgtggccct gaggcgtgcc agcaagaccc tcgccgaccg ctgcgtcctc
      721 gggataacgg cgaaccagga caagtgccgc gaatacgtcg agtgcagcat aggcctcgtc
      781 acggccctca acccctacat cggctacgag cgctccacgg agatagccca cgaggccctg
      841 gcaacgaacc gctcggtctg cgagctcgtc ctcgaaaagg gctacctgaa aaaggaagaa
      901 ctcgacgaca tcctctcccc ggagaacatg atcaaaccga ggtatatgga gaagaagtag
      961 aagacggcct ctcgcccgtt cgttcctcac tcaagcccgc ggaggacgca aagaaaagat
     1021 ggcggccaga ttccggagcc taccggaatc tggccgcatt tccgtcgcct tgcggcgagc
     1081 tgatcaccct tgttatgcct tccctcaaga gggttgtgtt gaagttgatt agcagtccta
     1141 agggttttcc tgccaatctc aggtacgtca ataattgctt cttgtggact ggctgaatct
     1201 cctcgacgga ctttagctcc acaatgatcg tgtcctccac cagcagatcc agcctcagac
     1261 cgacattggg aattttccgc cctttatagg acacgtgcag gggaacttcc gtcaggacct
     1321 ctatgcccat cagccccagc tctatgacca tggaggactg atagatgctc tcaagaaggc
     1381 ctggtccgaa caccttatgt acattgattg cggccctgat gaccagcgac gacagattat
     1441 ccacatcaaa ccttttcata ccaacacctc gtgtgagcgt attacttcga atctcgggtt
     1501 ttttgtcccg ccagcgtggc ggcgggatgc ctcccgtcga agacgggatg caagtgcggc
     1561 cagattctgg taggctccgg aatctggccg caattctttg cgcatttctc tgtgtcctct
     1621 gcgggcttga gtgaggaacg aacgggcgtg agataggtct ttgtcttgtt ccttgtctta
     1681 ttccgaagtt gtgctaaata gagtataagg gtggcacgaa agaaggaggt gatcggcgtg
     1741 tctatcatgg aagcgtcccc cacaaaggaa ttcgatcttt ttcaaggagt cagtcagagg
     1801 gttatcgggg agatcgggag gctgggagag gaggtgtcct tcgatcgcga cgacattgtt
     1861 ttcaaggcgg atgaaccgg
//
LOCUS       DAOPOA010000512         1878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_66543_length_1878_cov_4.481075, whole genome shotgun sequence.
ACCESSION   DAOPOA010000512 DAOPOA010000000
VERSION     DAOPOA010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66543_length_1878_cov_4.481075"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="PKJ17_09880"
     CDS             <1..1363
                     /locus_tag="PKJ17_09880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD76327.1"
                     /translation="AMIFVIHDSETGAPLYFANTSRDITDRKRAEEALLRARDELEER
                     VLARTAELEKAYMSLKCETEERRSLEERLRQSQKMEAIGTLAGGIAHDFNNILAGIIG
                     FTEMVEEDLPEDSHLREYVGRILKASLRGRDLVKQILTFSRKTEHVRVPVSLSPIINE
                     TCNLLRASLPAAIEIVLDVKSAADQVKASAVELQQILMNLATNAAHAMMKRGGTLRIS
                     LDEVDLAPPLPSGDSEIKPGRYLELVVTDTGDGMTPAVMKRIFEPFFTTRRPGEGTGM
                     GLAVVYGIVKSLGGTVSVKSEPQVGSTFRVLLPGAAGEREAEVPAAHGVPGGTERVLF
                     VDDEDLLAELAHDILESLGYRVTAVTDSTQALRVFSSDPASFDLVITDQIMPGFTGLN
                     LAKHFLDIREDIPIILCTGHSATVSPEIAKEAGIRAFVMKPLSKEDLAEVIRAVLDVG
                     TVQ"
     gene            1494..>1878
                     /locus_tag="PKJ17_09885"
     CDS             1494..>1878
                     /locus_tag="PKJ17_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD76328.1"
                     /translation="MKVHFWGTRGSLPAAASTLDLKRKVFGAIKSLAGRHLAGDDDVR
                     EFLEKELPFHLAGHYGTNTPCVEIRGGTEHVICDAGTGIRDLSRTLGVAPDGDPGERV
                     FNIFLSHLHWDHIQGFPFFMPAYSEG"
BASE COUNT          365 a          551 c          588 g          374 t
ORIGIN      
        1 tgccatgatc tttgtcatcc acgactccga gaccggcgcg cccctttact tcgcaaatac
       61 ttcgcgggat atcaccgacc gcaagcgcgc cgaggaggcg ttgctcaggg cccgcgacga
      121 actcgaagag cgcgttctcg cgcggacggc ggaactggag aaggcctaca tgtccctcaa
      181 atgcgagacg gaagaacgcc ggtcccttga agagcgcctc agacaatccc agaagatgga
      241 ggccatcggc actctggcgg gagggatcgc ccacgatttc aacaatatcc ttgccgggat
      301 catcgggttc acggaaatgg tggaggagga cctccccgag gacagccatc tgcgggaata
      361 cgtcggaagg atcctcaagg catccctcag ggggcgggac ctcgtgaaac agatcctcac
      421 cttctctcgc aagaccgaac atgtgagagt gcccgtctct ttgtctccca tcatcaacga
      481 gacctgcaac ctcctcaggg catctctccc ggccgccata gagatcgttc tcgatgtgaa
      541 gtcagccgcg gatcaggtca aggcgtccgc cgtcgaactc cagcagatcc tcatgaacct
      601 tgccaccaat gccgcgcatg cgatgatgaa gaggggaggg accctccgta tctccctgga
      661 cgaggtcgat ctcgcgccgc ccctcccgtc cggggattcc gagatcaaac cgggcagata
      721 tctggaactc gtggtgaccg acacgggaga cggcatgacc cccgccgtga tgaagcggat
      781 ctttgaaccc ttctttacaa cccgcaggcc cggtgaagga acgggcatgg gccttgccgt
      841 cgtctacggg atcgtcaaga gccttggcgg caccgtcagc gtcaagagcg agccgcaggt
      901 gggttccacc ttccgcgtcc ttttgccggg ggctgccggg gagagggaag cggaagtgcc
      961 tgctgctcac ggtgttcccg gcggtacgga aagagtgctc ttcgtagacg atgaggacct
     1021 ccttgccgag ttggcccacg atattctgga gtcgctcggg taccgggtca ccgcggtgac
     1081 ggacagtacc caggcgttga gggtgttctc gtccgatccc gcctctttcg atctcgttat
     1141 taccgaccag atcatgccgg ggttcaccgg tctcaatctg gcaaaacact tcctggatat
     1201 acgggaggat atccccatca tcctctgcac gggtcacagc gcgacggtat cgccggagat
     1261 cgcgaaagag gccggcatca gggcgttcgt gatgaagccc ctttcgaagg aggacctggc
     1321 ggaggtcatc cgagccgtgc tcgatgttgg aaccgtgcag tgacgctcag agggtgtgac
     1381 tgcagttctg tttcggtccg gtagcttccc ggattgtcct tgtaatttcg cttctccacg
     1441 actattgtat tggtattccg ggctgcgcat cgtggccggg acggaggttc ctgatgaagg
     1501 tgcatttctg ggggacgcgg gggtcgctgc cggcggccgc gagcacgctg gacctgaaac
     1561 ggaaggtgtt cggggccata aagagtcttg cggggcgaca tttggcaggc gatgacgatg
     1621 tccgggagtt cctggagaaa gagctcccct ttcacctggc gggtcactat ggcacgaaca
     1681 cgccctgtgt cgagataagg ggtggaacgg agcatgtgat ctgcgacgcc ggtacgggga
     1741 tccgggacct ttcgcgcacg cttggcgtcg cgccggacgg cgatccgggc gaaagggtct
     1801 tcaacatttt cctgtcccac ctgcactggg accatatcca gggatttccg ttctttatgc
     1861 ctgcttactc ggaaggga
//
LOCUS       DAOPOA010000513         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_66591_length_1877_cov_4.060922, whole genome shotgun sequence.
ACCESSION   DAOPOA010000513 DAOPOA010000000
VERSION     DAOPOA010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66591_length_1877_cov_4.060922"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="PKJ17_09890"
     CDS             <1..1687
                     /locus_tag="PKJ17_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD76329.1"
                     /translation="RELMKKGGVLEYDGSVTDPSGCAHDVIIRKAAFHKPDGSPGGIV
                     GVVIDVTELKRAGEMLKESEAKYRTVAEESLAGFYIIQDGLFRYVNRRFCEITGYSYD
                     EIVDRMEPLDLVYGEDRDVVEENLRKRLGSEAGSIQYRFRMTRKSGLIARVEALGSSF
                     DYRGRPAAIGTLLDITKETILEEQLQQAQKMEAVGQLAGGMAHDFNNILTTIMGYCGL
                     LQTELDEDDPRQLYAEEISMASEKGAQLTSGLLAFGRKQVMEMKPQMLGDLIQGVQRL
                     LERLLPEDIRLEIRLPREDCTVLADATQIYQLLMNLTTNSRDAMPEGGTLLISADDLS
                     IDEDFIALKGFGEAGHYAVLSVADTGCGIDLRVQEKMFEPFFTTKEPGKGTGLGLSIV
                     YGIVKQHGGFIDVCSAPGAGTTFTVYLPVAKGRPERRRAGVQPMRGGSETILIAEDNR
                     SVRELTREILRKAGYRIIEAVDGQEAIERFTEFSHVIDLSILDVVMPKRNGKQVYDEI
                     IMMRPDAKVLFVSGYAADVITDKGIRDQSLEYLSKPVSPTLLLKKVRELLDRK"
BASE COUNT          426 a          524 c          576 g          351 t
ORIGIN      
        1 cagggaactg atgaagaaag ggggcgtgct cgagtatgac gggtcggtca ccgacccttc
       61 ggggtgcgcc catgacgtca taatccggaa ggccgccttc cacaagcctg acggctctcc
      121 cggaggtatt gtcggcgtcg tcatcgacgt aacggaactt aagagggccg gagagatgct
      181 taaggagtcg gaggcaaagt accgcaccgt cgccgaggag tccctggccg gcttctatat
      241 catccaggac ggtctcttcc ggtacgtgaa caggaggttc tgcgagatca cgggttatag
      301 ctatgatgag atcgttgaca ggatggagcc tctcgatctt gtgtacggag aagaccggga
      361 tgttgtggaa gagaacctga ggaagcgcct tggtagtgag gcggggagta tccagtacag
      421 gttccggatg acgcgcaaga gcggcctcat cgccagggtg gaggcgctgg ggagttcatt
      481 cgactatcgg ggcagaccgg ccgccatcgg gaccctcctc gatatcacga aggagaccat
      541 actcgaggag cagcttcagc aggcccagaa aatggaggcc gtcggacagc tggccggggg
      601 catggctcac gacttcaaca atatcctcac caccatcatg ggttactgcg gtctcctcca
      661 gaccgagctg gatgaagatg atccccgtca gctctatgca gaggagatca gcatggcatc
      721 cgagaaggga gcgcagctca ccagcggtct tcttgccttc ggaagaaaac aggtcatgga
      781 gatgaagccc caaatgctgg gggatctaat acaaggggtg cagagactcc tcgagaggct
      841 cctccccgag gacatcagac tggagatccg ccttccccgc gaggattgca cggtccttgc
      901 cgacgcgaca cagatatatc agcttctcat gaacctgacg acgaactccc gggacgccat
      961 gcccgaaggc gggaccctgc tcatctcagc cgacgaccta tcgatcgatg aggatttcat
     1021 cgccctgaag ggatttggag aggcgggcca ttatgcggtc ctctccgtcg ccgatacggg
     1081 ttgcggcatc gacctccgcg tacaggagaa aatgttcgag cctttcttca ccacgaagga
     1141 gccgggaaaa ggcaccggtc tcggcctctc catcgtttac ggtatagtca agcagcatgg
     1201 cggttttatc gatgtctgca gcgcccccgg ggcaggaacg accttcacgg tctacctccc
     1261 cgtcgcgaag gggagaccgg aacgcagacg cgccggcgtt cagcccatgc ggggcggcag
     1321 cgagacgatc ctgatagccg aggacaacag gagcgtccgt gagcttacgc gcgagatcct
     1381 gcggaaggcc ggttatcgca taatagaggc ggtcgacgga caggaggcca tcgagcgttt
     1441 caccgagttc tctcacgtga tcgacctatc gatcctcgac gtggtcatgc cgaagagaaa
     1501 cggcaaacag gtgtatgacg agatcatcat gatgcggccc gatgcgaagg tgctctttgt
     1561 cagcgggtat gcggccgatg tcatcacaga caaggggata cgcgaccaat ccctggaata
     1621 cctctcgaaa cccgtgtccc cgaccctgct cctcaagaaa gtgagggaac ttctggacag
     1681 gaaataaaga aggttcaaga gctccagagt tcaagagtaa aagacaaaaa gggcaccggc
     1741 gccttcggcc ggtgtacgta ttcccttgac agccgacccc ccttcgcggt ctttcaaatc
     1801 ttgagtatca gttccccccg ggagcgtttc ggcacgtggt ggactttgtc ggcgtcgcgc
     1861 cagtacttga tgtcccc
//
LOCUS       DAOPOA010000514         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_66685_length_1875_cov_2.791209, whole genome shotgun sequence.
ACCESSION   DAOPOA010000514 DAOPOA010000000
VERSION     DAOPOA010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66685_length_1875_cov_2.791209"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PKJ17_09895"
     CDS             <1..1003
                     /locus_tag="PKJ17_09895"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD76330.1"
                     /translation="RVTRNCPWNQCIFCPAYKGVKFSRRTVDEVKKDIDSMEKEYASY
                     KNTIRSAFLQDADSLVLKTSDILEIIGYTKEKFPGLERITTYARATTLKRKTVEELRE
                     LCKAGLTRIHVGLESGSEKVLKMIRKGITPEDIVEGGRKVVEAGISLSEYIMPGVGGR
                     TMSSENAVETARLLNHIKPDFIRVRTFAMHPMSPMHKLVEDGTFVVMNDAEIVAEIRL
                     LVENLDEMHSYFSCADFSLNLLMQVDGYLDEKKDYMLSELDRFLALTPEQQKAYALIR
                     RSSYMQYPLEAAENEDLLSRIRPEIEKLEKEDPEGFYRYIETLKSYQLPQPQTDEWK"
     gene            complement(1167..>1875)
                     /locus_tag="PKJ17_09900"
     CDS             complement(1167..>1875)
                     /locus_tag="PKJ17_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76331.1"
                     /translation="QITDNIDTFERWEGADHTDTEHWRAIVHLYDQLDVISGHDDLVG
                     EHAHPHYCEQLSRVLKEEPAGDMGRSPSFDEETAILRDCAAALHDVYDMQLWADLKHI
                     QYGLNWKVLESVLPRYSHSTALSGEDAALNKNLLGLLNRLVSIGKKSCPRLPMQDRHI
                     DFVLDIVRKKYCQEDIDRSISHIAQCLRNGIDCYLTMDGELAERFTESKQNLQLALGA
                     SIHLEVLRPTELEKRLG"
BASE COUNT          425 a          531 c          528 g          391 t
ORIGIN      
        1 acgggtgacg aggaattgcc cctggaatca gtgcatcttc tgtcccgcct acaagggagt
       61 gaaattctcc cgccgtacag tcgacgaggt caagaaggac atcgatagta tggagaagga
      121 gtacgcctcc tacaagaaca cgatcagatc ggccttcctc caggatgccg acagcctcgt
      181 actcaagacc tcggatattc tcgagatcat cggatatacg aaggaaaagt ttcccggcct
      241 tgaacgcatc acgacctatg cccgggcaac gaccttgaaa cgcaagaccg tggaagagtt
      301 gcgcgagctc tgcaaggccg gcctcacgag gatacacgtg ggactggaaa gcggctcgga
      361 gaaggtcctc aagatgatac ggaaaggtat cacacccgag gacatcgtcg aaggcggcag
      421 gaaggtcgtg gaagcgggga tatcgctgtc ggaatacata atgcccggcg tgggcggcag
      481 gacgatgagc tccgagaacg ccgtcgagac ggcgcgactt ctcaaccaca tcaagcccga
      541 cttcatcagg gttcggacct ttgccatgca ccccatgtca ccgatgcaca agctcgttga
      601 ggacgggacc tttgtcgtca tgaacgacgc ggagatagtg gcagagatac gtcttctcgt
      661 cgaaaacctc gatgagatgc actcttactt ctcctgtgcc gatttcagcc tcaacctgct
      721 catgcaggtg gacggctatc ttgacgaaaa aaaggactac atgctcagcg agcttgacag
      781 gttcctcgcc ctcacaccgg aacagcagaa ggcctatgct ctcatcaggc gttcctccta
      841 catgcagtat cccctcgagg ccgccgagaa cgaggacctt ctgagcagga tacgccccga
      901 gatcgaaaag ctcgaaaagg aggaccccga gggcttctac aggtacatag aaacgctgaa
      961 atcctaccag ctcccccaac cacagacgga tgagtggaaa taaaaaaagg gttcaagggt
     1021 tcaagggttc aagggttcaa gggaagaacc gcgagggagg ccgtttgtca acacaattgc
     1081 cgaccagggc acaccggccg aaggcaccgc tgcccttttt gtcttttact cttgaactct
     1141 tgaacccttg aacccttgaa ccttctttac cccagtcttt tctccagttc cgtcgggcgc
     1201 aggacctcga ggtggatgga tgctcccagg gcgagctgga ggttctgttt gctttcggtg
     1261 aatctctccg ccagttcacc atccatcgtg aggtagcagt cgataccgtt ccggaggcac
     1321 tgggctatgt gcgagatgct gcggtcgatg tcctcctggc agtacttctt tctcacgata
     1381 tcgagaacga aatcgatatg ccggtcctgc atgggcagcc gggggcagct cttcttcccg
     1441 atgctgacga gccggttgag aaggccgaga aggttcttgt tcagcgccgc gtcctcgccg
     1501 ctcagtgcgg tgctgtggga atatctcggc aagacggact caaggacctt ccagttgagg
     1561 ccgtattgga tatgcttgag gtccgcccag agttgcatgt catagacgtc atgcaacgcc
     1621 gccgcgcagt ccctgagtat cgccgtctcc tcatcgaacg agggcgatct ccccatgtcc
     1681 ccggcgggct cttccttcag gacacggctc agctgttcgc aatagtgtgg atgggcatgc
     1741 tctccgacaa ggtcatcatg cccgcttatg acgtcgagct ggtcatagag atgcacgatg
     1801 gcccgccagt gttccgtgtc tgtgtggtcc gccccttccc agcgctcgaa ggtgtcgata
     1861 ttatcggtga tctga
//
LOCUS       DAOPOA010000515         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_66827_length_1872_cov_3.528894, whole genome shotgun sequence.
ACCESSION   DAOPOA010000515 DAOPOA010000000
VERSION     DAOPOA010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66827_length_1872_cov_3.528894"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKJ17_09905"
     CDS             <1..430
                     /locus_tag="PKJ17_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76332.1"
                     /translation="VIDRPDKVTVLFAGPKGTDDYYTTVTVQNVASAKYGGTHQNVGS
                     LVSDLKEQFRKSDPNTRFFSEKTFAFTSEDATPVNAITFSVTYALQGEKFQQWAVIIP
                     RASDLVYHVFFYTSPVKLYERNARTAYDMLQTLRLVKQGK"
     gene            566..1114
                     /locus_tag="PKJ17_09910"
     CDS             566..1114
                     /locus_tag="PKJ17_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76333.1"
                     /translation="MKRFVLFAMCAALSGLWACQTLGPVGSPGPSAPPPPAYTCEKKD
                     IVPVRARTQYSDDYGTMMTSDAAALSTPARNYRWGIKNSKTLSMAYFTFYVRPEGRYR
                     YFKTSIYIDRGVKAPMTFLVRNNDRKGDVLKSVTVYPGQTVDVDVEISGARRLNVWSE
                     LRINHDKADKIIFGEPEFYNCR"
     gene            1384..>1872
                     /locus_tag="PKJ17_09915"
     CDS             1384..>1872
                     /locus_tag="PKJ17_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOD76334.1"
                     /translation="MKREFEEESAIFASIAKWFLLATLTGTVIGLVSAGFLKALNFSI
                     RLMEGHHHYFLLLPAGLLISVFIRKMTGDETKGLGAEIEAVHKKSGKIKPRAVFLKLV
                     ATIVTVATGGSAGKEGPCAEIGAGISSILSDVLRLSKYDRKKLVICGISAGLAAVFGT
                     PIS"
BASE COUNT          427 a          508 c          561 g          376 t
ORIGIN      
        1 ggttatcgac aggcccgaca aggtcaccgt gcttttcgcg ggccccaagg gtaccgacga
       61 ttactatacg acggtgaccg tgcagaacgt tgcctcggcg aagtacggag gcacccacca
      121 gaacgtcggc tccctggtct ccgacctcaa ggagcagttc agaaagagcg acccgaatac
      181 acggttcttc agcgaaaaga ccttcgcctt cacgtcggag gatgcgacgc ccgtgaacgc
      241 gatcaccttc agcgtcacgt acgcgctgca gggagagaaa ttccagcaat gggccgtgat
      301 catcccccgc gcctctgatc tcgtgtatca tgtcttcttc tacacgtcgc ccgtcaaact
      361 gtacgagagg aacgcgagaa ccgcctacga catgctgcag acccttcgcc tggtcaaaca
      421 ggggaagtag aaggccgttg aagcgaatca agccgcccgg gcaactcgag ccaccttttc
      481 gttttctctt gcatccgggt ggtgattcgg agagaatgaa gctacaacgt tcatccaggg
      541 tggagagaaa aaaggaggga tctgaatgaa aagatttgtc ttgttcgcga tgtgcgcggc
      601 tctgtccggt ctatgggcct gccagaccct cgggcccgtt gggtcacctg gcccgagtgc
      661 tccgccgccg ccggcttata cctgcgagaa gaaggacatt gtgcccgtga gagccaggac
      721 acagtacagc gatgactacg ggacgatgat gaccagcgac gccgcggcgt tgagcacccc
      781 ggcgaggaac taccgttggg gcataaagaa ctcaaagacc ctgtccatgg cctactttac
      841 cttttacgtc aggcccgagg gcaggtacag gtatttcaag acatccatat atatagacag
      901 gggcgtgaag gcccccatga cattccttgt aaggaacaac gacaggaagg gggatgtcct
      961 gaaaagcgtg acggtctacc cgggccaaac ggtggatgtt gatgtcgaga tcagcggggc
     1021 caggagactg aacgtgtggt cggaactcag gataaatcac gacaaggccg acaagatcat
     1081 cttcggcgaa cccgagttct ataactgcag ataactttgt tcgatatagc tgcatctttc
     1141 aggaatctct cttaaggttt cggaagctgc gtatctttgc gggaaggacg taccggtccg
     1201 ccggggcgtc ttctctcccc gtcgtcccgg tcacccagct gtgcgaacgc ggtctcgcag
     1261 cggcagggca cagacggaag acccggggtc ctgcaggatg agcagcttcc gaaacctgtt
     1321 tatgcagtga ccggtacgcc gcgataatac gatacaggat cggtacatga ggagtgcttc
     1381 ggcatgaaga gggagtttga agaagaatcc gccatattcg ccagcatagc caaatggttc
     1441 ctccttgcta cgttgaccgg tacggtcatc ggtctcgtgt cggcgggttt cctgaaggcc
     1501 ctgaacttca gcattcggct gatggaaggg caccatcact acttcctcct gctccccgcg
     1561 ggtcttctca tcagtgtctt catcaggaag atgacgggtg atgagacgaa aggcctgggt
     1621 gcggagatcg aggcggtgca taagaaatcc ggcaagatca agccccgtgc cgtcttcctg
     1681 aaacttgtcg cgacgatagt gacggtggcg acgggaggat cggcgggaaa ggaagggccc
     1741 tgcgcggaga taggggccgg catctcatcg atcctgtccg atgttctgag gctcagcaag
     1801 tatgacagga agaagctcgt catatgcggg ataagcgccg gtctggcggc tgttttcggt
     1861 accccgatct cg
//
LOCUS       DAOPOA010000516         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_66945_length_1870_cov_2.677135, whole genome shotgun sequence.
ACCESSION   DAOPOA010000516 DAOPOA010000000
VERSION     DAOPOA010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66945_length_1870_cov_2.677135"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1558)
                     /locus_tag="PKJ17_09920"
     CDS             complement(92..1558)
                     /locus_tag="PKJ17_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOD76335.1"
                     /translation="MKNKRLVVTVCVLFAAALAFAYVRAQVLPEARPAAPQVQNVRAV
                     AAFNGSGLPSLAGLVNEAKPSIVNISTTAVVRGPGGQGPMMGPNNPFKDFFGDDFFDK
                     FFGNGPRREYKQRSLGSGFIIDKEGYILTNNHVVEKATTIKVKLSDEKEYDARIIGRD
                     PKTDIALIKVDVRQSLPVAVLGDSEVLQVGDWVLAIGNPFGLEHTVTAGIVSAKGRII
                     GAGPYDQFIQTDASINPGNSGGPLLNLKGEVVGINTAIVSGGQGIGFAIPINVAKDML
                     SQLKSKGKVARGWLGVVIQRMTPEIAKSFGLKESEGALVADVMDQSPADKAGIRRGDV
                     IISYNGKKIKDNETLPRLVAATEIGTKAKIILVRDNKQMEVSVTIGELQEEGLRASKK
                     TEIEKDLGLVVQDITAEIAKHLNLKDRKGVIVTDVIPGSPAQEADIRSGDVIREIGRK
                     PVRSVAEFKEAVKRSNVKEGIVMLIQRENATFYAVIRE"
     gene            complement(1572..>1870)
                     /locus_tag="PKJ17_09925"
     CDS             complement(1572..>1870)
                     /locus_tag="PKJ17_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD76336.1"
                     /translation="VENGIKYGHPGGHVTVSSYKQNGFACINVEDDGPGISGEDIKYI
                     FDRFYRADRSRKRESGSGLGLSISKWIAEAHKGTIEVESKPAAGSLFTIKLPMS"
BASE COUNT          310 a          581 c          529 g          450 t
ORIGIN      
        1 ggtcacattt tacccgtccc gggccgcagg ccccagggag tctttgcctc tcacctgaga
       61 cctgcaacct gaagaaacgt gaaaccgttt tctactctct tatcaccgcg tagaacgtcg
      121 cgttctcgcg ctggatcagc atgacgatgc cttccttcac gttggagcgc ttgaccgctt
      181 ctttgaactc cgcgacgctt ctgacgggct ttctcccgat ctccctgatg acgtcgccgg
      241 agcggatgtc tgcctcctgg gccggtgaac cggggatgac atcagtgacg ataacgccct
      301 ttctgtcctt caggttgagg tgcttcgcga tctcggcggt aatgtcctga acgacgagtc
      361 cgaggtcctt ttcgatctcc gtctttttcg atgccctgag gccctcttcc tgcagttcgc
      421 cgatggtcac gctcacttcc atctgtttgt tgtccctgac aaggatgatc tttgccttcg
      481 tgcctatctc cgtcgccgcg acgagccggg gaagggtctc gttgtccttg atcttcttgc
      541 cgttgtaaga gatgatgacg tctccacgcc tgatgcccgc cttgtccgcg gggctctggt
      601 ccatgacatc ggcaacgagg gcgccctcgg attccttcag cccaaagctc ttcgcgatct
      661 caggcgtcat cctctggatg accacgccga gccagccgcg ggcgaccttg cccttgcttt
      721 tcaactggga cagcatgtcc tttgccacat tgatgggtat ggcaaaaccg atgccctggc
      781 cgccggagac aatggccgtg tttatcccca ccacttcccc tttgagatta aggagcggcc
      841 cgccggagtt gccgggatta atggaggcat ccgtctggat gaactgatcg tacggtcccg
      901 ctccgatgat cctgcccttc gcgctcacga tgcctgcggt gacggtgtgt tcaagaccga
      961 agggattgcc gatggcgagg acccagtctc ccacctggag cacttcggaa tcgccaagca
     1021 cggcgacggg aagggactgc ctgacgtcga ccttgatgag ggcgatgtcc gtcttggggt
     1081 cccgccctat tatcctggcg tcatactcct tctcgtcgga cagctttacc ttgatggtcg
     1141 tggccttttc gaccacgtga ttgttcgtca gtatgtaccc ttccttgtcg atgatgaatc
     1201 cggagccgag gctgcgctgc ttgtattcgc gccgtggccc gttaccgaag aacttgtcga
     1261 agaaatcgtc gccaaagaaa tccttgaagg ggttgttggg gcccatcatc gggccctgcc
     1321 ccccgggccc tctgacgacc gcggtggtgc ttatgttgac gatcgacggc ttcgcctcat
     1381 tgacgagccc cgcgaggctg ggcaacccgc tgccgttgaa agccgccacg gcccggacgt
     1441 tctgtacctg cggtgccgcg ggcctcgctt cgggcagcac ctgtgccctg acataggcaa
     1501 aggccagggc cgccgcgaaa aggacacaaa cggttacgac gagtcgtttg ttcttcatgg
     1561 aatatcctcc gtcatgacat gggcagtttg atggtgaaca ggctccctgc ggcgggcttc
     1621 gattccacct cgatggtgcc cttgtgggcc tcggctatcc atttgctgat ggaaagaccg
     1681 agaccgctgc cgctttcccg cttgcgcgac ctgtccgcgc ggtagaacct gtcgaagatg
     1741 tactttatgt cctcccccga tatgccggga ccgtcgtcct cgacattgat gcaggcgaag
     1801 ccattttgtt tgtaggatga gaccgtcacg tgcccgccgg ggtgtccgta cttgatgccg
     1861 ttctccacca
//
LOCUS       DAOPOA010000517         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_67053_length_1868_cov_2.879757, whole genome shotgun sequence.
ACCESSION   DAOPOA010000517 DAOPOA010000000
VERSION     DAOPOA010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67053_length_1868_cov_2.879757"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PKJ17_09930"
     CDS             complement(<1..614)
                     /locus_tag="PKJ17_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOD76337.1"
                     /translation="MRIDKRRYYHLDWYLIINGLLIFGIGLLNLLSATSSFSSGSYNF
                     MIKQLIAFAMGLVLIGVVMYYDYRVIANQSRWIYLGGLCLVILVLIIGLAAGGAKRWI
                     SLFGFSIQPSELMKPILVIFLANMLHEKKRQNIPLSLKDIGKPLLWTLIPFVLIVKQP
                     DLGTGIVIILVCFAILWYVGLKRSTYAVIFGVGAISPFFAWNYL"
     gene            complement(601..>1868)
                     /gene="mrdA"
                     /locus_tag="PKJ17_09935"
     CDS             complement(601..>1868)
                     /gene="mrdA"
                     /locus_tag="PKJ17_09935"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOD76338.1"
                     /translation="KYKDYSPGDYIGKYGLERSYENDLRGVDGEKRVEVDAIGREVRT
                     LDVVDPIPGHSLHLNLDLELQLSADKALEVRKGAAVALNPKTGGVLVLASRPGFDPNL
                     FASGISKKEWQKIALDKAHPLQNRAIQGGYPPGSTFKILTAMQALELGVINERTTFTC
                     GGGFAYGNRVFKCWKKGGHGTTSVHRAIVESCDVFFYNVGLKLGVDRIHEFGTAVGLG
                     RVTGIDLPNEQKGLLPSTEWKKKRYNQPWYEGETVSVAIGQGAVWLTPVQLAQLSSFV
                     ANDGKNFKPQIVNRIVSTEGKVVKTFEPVVNADVKFKTGVLKIVKDGMRGVVNEPGGT
                     AGASRLQNVLMCGKTGTAQAGSDKVKLGDHAWFIAYAPAEDASIAMAILVEHGLHGSS
                     AAAPIAKGITETLFKVKTVIKEAKADENR"
BASE COUNT          363 a          549 c          548 g          408 t
ORIGIN      
        1 atgaggtagt tccaggcaaa aaagggcgat atcgccccga cgccgaaaat gaccgcgtag
       61 gtcgatctct tgaggcccac ataccacagg atggcaaaac agacgaggat tatgacgatg
      121 cccgtgccga ggtccggctg cttcacgatg aggacgaagg gtatgagggt ccacaggagc
      181 ggtttgccga tgtccttcag ggacagcggg atattctgcc ttttcttctc gtgcagcatg
      241 ttcgccagga aaatcacgag gatgggtttc atgagctccg agggctggat gctgaacccg
      301 aagaggctta tccatctctt ggcgccgccc gccgcgagcc ctatgataag gacgagtatg
      361 acaaggcaga gccctccgag atagatccat cgagactgat tcgctatgac gcgataatca
      421 tagtacatga cgacgccgat gaggacaagg cccatggcaa aggctatgag ttgcttgatc
      481 atgaaattgt aggagccgct ggagaatgaa ctggtggcgc tcaggaggtt caaaaggccg
      541 atgccgaata tcagcagccc gttgatgatg aggtaccagt cgaggtggta gtatctcctt
      601 ttatctattc tcatctgcct tcgcttcctt tatgactgtt ttgaccttga agagggtctc
      661 cgtgatgccc ttggctatcg gcgcggccgc ggacgagccg tggagcccat gctccaccag
      721 gatggccatg gcgatggatg cgtcctcggc aggcgcgtac gcgatgaacc aggcgtggtc
      781 gccgagtttc actttatccg aaccggcctg ggccgttccc gtctttccgc acatgagcac
      841 gttctgcaag cgtgaggccc ctgcggtgcc tcccggctcg ttcacgaccc cccgcattcc
      901 gtccttgacg atcttgagaa ccccggtttt gaacttcacg tcggcattga cgacgggctc
      961 gaaggtcttg accaccttgc cctccgtcga cacgatgcgg ttgacgatct ggggtttgaa
     1021 gttcttgccg tcgttcgcaa cgaaagacga caactgcgcc agctggaccg gcgtcagcca
     1081 gacggccccc tgtccgatcg cgacggaaac ggtctcacct tcgtaccagg gttgattgta
     1141 tcttttcttc ttccattccg ttgacggcag gagccccttt tgctcattgg ggaggtcgat
     1201 ccccgtcacg cggccgaggc cgacggcggt gccgaactca tgtatgcggt ccacgcccag
     1261 cttgaggccg acattgtaaa agaagacatc acaggattcc acgatggcgc gatggaccga
     1321 ggtcgtcccg tgccccccct tcttccagca cttgaagacg cggttgccgt aggcgaaccc
     1381 gccaccgcag gtgaaggtcg tgcgctcgtt gataacaccc agttcgaggg cctgcatcgc
     1441 cgtgagtatc ttgaaggtcg accccggcgg atacccgccc tgaatggccc tgttctgaag
     1501 gggatgcgcc ttgtcgaggg ctatcttctg ccattccttc ttggatatcc ccgaggcgaa
     1561 aaggttggga tcgaagcccg gccggctcgc gaggacgagg acgccgcccg tcttcggatt
     1621 aagcgccacc gcggcacctt ttcgtacctc cagggccttg tcggcggaga gctgaagctc
     1681 cagatccagg ttcaggtgca ggctgtgtcc cggaatcggg tcgacgacgt cgagggtgcg
     1741 tacctcgcgc ccgatggcgt ccacctcgac ccgcttttcc ccgtctaccc ccctgaggtc
     1801 gttctcgtaa gacctttcga ggccgtattt ccctatataa tcgcccgggg aatagtcctt
     1861 gtactttt
//
LOCUS       DAOPOA010000518         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_67148_length_1866_cov_3.581447, whole genome shotgun sequence.
ACCESSION   DAOPOA010000518 DAOPOA010000000
VERSION     DAOPOA010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67148_length_1866_cov_3.581447"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKJ17_09940"
     CDS             complement(<1..425)
                     /locus_tag="PKJ17_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOD76339.1"
                     /translation="MEASSEFSSRPRSTGEFLRRTVVYLKPYKAAAAGNMALAFLTIG
                     FYYVFPYVTQYIIDDVIGCGRLESLLPAIVSLLGTFLLCELSNALRIIVNTHFGQNVI
                     YDMRRDVYARMQRLPVSFFDRRFSGDLMTRVIDDVASVHR"
     gene            complement(434..1564)
                     /locus_tag="PKJ17_09945"
     CDS             complement(434..1564)
                     /locus_tag="PKJ17_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXXX repeat peptide maturase"
                     /protein_id="HOD76340.1"
                     /translation="MIHYLIVILEHDSAPFCCYDVKERGGPGLIPLDVLEKSVSFAIK
                     NAIPVNFLCGNTALPPGYEELIASVDHVRMVPLARARSGEEAVFIIDGEEDLAHAHVL
                     ETGGLNNVILRVGKSEVPSLPSTVERLLGRFRRLNVILKEPGGFAEEDFSAYADALED
                     VRRILAGEYRKGSFLEISTLSDRVFLTNMNNCNAGTAHLTVAPNGRFYLCPAFYYENG
                     EDCLAGPDDAVEIRNARLLTLENAPVCRNCDAWHCKRCVWLNRRLTSELNTPSHEQCV
                     LAHLEREASRRFLEEIGRECGLNDRVTPIPKLDYLDPFDIISDRSRSPGEKEEHFARL
                     LSRPLEDVPVSDLLRQIYTLDPGMLARLKELNSAVVDLEDGQ"
     gene            complement(1561..>1866)
                     /locus_tag="PKJ17_09950"
     CDS             complement(1561..>1866)
                     /locus_tag="PKJ17_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76341.1"
                     /translation="PFSLVNRKFPPVGNCFEGLDRNRLRPFLALDLVSQSRPECIDCE
                     VATGCAWCQGANYDCAETGTIYQRATHLCRMHKARVRANNYYWHRLEGARRPTREAQ"
BASE COUNT          347 a          547 c          604 g          368 t
ORIGIN      
        1 cggtgcacgg aagcgacgtc gtctatcacc cgcgtcatca ggtcccccga gaagcggcgg
       61 tcgaagaagc tcacggggag acgctgcatc cgcgcataga cgtcacggcg catgtcgtag
      121 atgacgttct gtccgaaatg ggtgttgacg atgatcctca gggcgttcga gagctcgcag
      181 agaaggaagg tcccgaggag gctcacgatg gccggcagca gagactcgag tctcccgcac
      241 ccgatgacgt cgtcgatgat gtactgcgtg acgtagggaa atacgtagta gaagccgatc
      301 gtgaggaacg caagggccat gttccccgcc gcggccgcct tgtagggctt gaggtacacg
      361 acggtccggc gcaggaactc gccggtggaa cggggccggg aggagaattc ggaagaggct
      421 tccatgggcc ccgctattga ccgtcttcga ggtcgacgac agcggagttc aactctttga
      481 ggcgcgcgag catccccggg tcgagggtgt agatctggcg gagcaggtcg ctcacgggga
      541 cgtcctcgag cggccgcgag aggagccggg cgaagtgttc ttccttttct ccggggcttc
      601 gcgaccggtc gctgatgatg tcgaaggggt cgaggtaatc gagcttcggg atcggtgtca
      661 ccctgtcgtt aaggccgcat tcccggccga tctcttccag gaatctccgt gacgcctcgc
      721 gctccaggtg ggcaagaacg cactgctcat gcgacggcgt gttgagttcc gatgtcagcc
      781 tgcggttgag ccagacgcat ctcttgcagt gccaggcgtc gcagttgcgg cagacggggg
      841 cattctccag ggtgagaagc cgggcgttcc ttatctccac cgcatcatcg ggccccgcaa
      901 ggcagtcctc cccgttctcg taatagaagg cggggcagag atagaacctg ccgttgggcg
      961 cgaccgtcag gtgggccgtc cctgcgttgc agttgttcat gttcgtgagg aagaccctgt
     1021 ccgaaagggt gcttatctcg aggaagctgc cttttcgata ctccccggcg aggatgcgcc
     1081 ggacatcttc cagtgcatcg gcatatgcgg aaaagtcctc ttccgcaaat ccacccggtt
     1141 ccttcaggat gacgttgagc ctcctgaacc ttccgagaag tctctccacg gtggagggga
     1201 gcgacggcac ctcactcttg ccgacgcgga gaatgacgtt gttgaggccg cccgtctcga
     1261 ggacatgggc atgggcaaga tcctcttcgc cgtctatgat gaagacggcc tcttccccgg
     1321 accgggcgcg ggcgagagga accattctca cgtgatcgac gcttgcgatc agctcttcat
     1381 acccgggggg cagggccgtg ttcccgcaga ggaaattgac gggtatggcg ttctttatcg
     1441 cgaaagagac gctcttttcc agcacgtcga ggggtataag cccgggtccg cccctttcct
     1501 tcacatcgta gcagcaaaac ggggccgaat catgctccag gatgacgatg aggtaatgta
     1561 tcattgcgcc tccctcgtgg gccggcgggc gccttcaagc ctgtgccagt aatagttgtt
     1621 ggcccgcacc cttgccttgt gcattctgca caggtgcgtc gccctctggt agatcgttcc
     1681 cgtttcggca cagtcgtaat tggccccctg acaccaggca cagcccgtcg cgacctcgca
     1741 gtcgatacac tcggggcggc tctggctcac aaggtcaagg gccagaaagg ggcgcagcct
     1801 gttcctgtcg agcccctcga agcagttgcc caccgggggg aacttcctgt tcacgagaga
     1861 gaaggg
//
LOCUS       DAOPOA010000519         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_67423_length_1861_cov_2.646733, whole genome shotgun sequence.
ACCESSION   DAOPOA010000519 DAOPOA010000000
VERSION     DAOPOA010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67423_length_1861_cov_2.646733"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..612
                     /locus_tag="PKJ17_09955"
     CDS             85..612
                     /locus_tag="PKJ17_09955"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOD76342.1"
                     /translation="MNDLLYGIITGIIFGFLLQKARVIRYDKQLAALRLMDMTIVKYM
                     FTTVLVAMVGVYLLKDLGLVKLSIKTTILGGNIIGGLIFGAGWGLLGYCPGTSLGALG
                     EGRWDSIWGIVGMLAGAAVFAELFPLMKRTVLTWGNLGKITIPGILGINHWIVIVIVV
                     VLAVLMFRFFEKKGL"
     gene            846..>1861
                     /locus_tag="PKJ17_09960"
     CDS             846..>1861
                     /locus_tag="PKJ17_09960"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD76343.1"
                     /translation="MSAERLVVIGGVAAGMSAASSFKRLKPEAEAIVLQNDHFISYGA
                     CSLPYYISDDVKDINRLISLSPEVATKERGITVLTRHEARSIDPARKTVTAVDLDGNK
                     EKTISYDHLVVATGGLPVRPPFPGIDLGHIFTLRTLHDGIEIKRFIDRWTTFEPCVGP
                     QCVYVNPFGAEKRPMRASIVGGGSIGMEMCESLRKRRVEVTVFEKMDRVLGTMDTSIT
                     NIVEERLAAEGVNLMKGVSVQSFEGDSGSVTRVITDKGTFDTDMVLLVIGARPNTKIA
                     VDAGIELGAGGAIKVDDHLRTNIPDIYAAGDCAEATQMVTGKKAYIPLGATATLYVGN
                     TEQI"
BASE COUNT          418 a          523 c          551 g          369 t
ORIGIN      
        1 gatgctgatc ggcatctccc tgatctgttt cttcgttggc ggcctcatag tcgcccgcat
       61 cctttatcag ggaggtgcca aaaaatgaac gatctcctct atggcatcat aacaggcatc
      121 atcttcggct ttcttcttca gaaggcccgg gtcatacgct acgacaagca actggcggcc
      181 ttacggctca tggacatgac catcgtcaag tacatgttca caacggtgct cgtagccatg
      241 gtgggcgtct atcttctcaa ggacctgggg ctcgtaaaac tctccatcaa gacaacgatc
      301 ctggggggca acataatagg cgggctcatc ttcggcgcag gctgggggct tctgggatac
      361 tgccccggga catccctcgg cgcgctgggc gaagggcgct gggattcaat atgggggatc
      421 gtcggcatgc tcgcgggcgc cgcggtcttt gccgaacttt ttcctctcat gaaaagaacg
      481 gtcctgacct ggggaaacct cggtaagatc accatcccgg gcatcctggg catcaaccac
      541 tggatcgtca ttgtaatagt ggtggtactg gcagtcctga tgttcaggtt cttcgaaaag
      601 aaggggctgt aacagacagg ttgtaggtta taggcaatgg gtgacgggtg atcgctgcaa
      661 ggaccgtggt tttccggaca ttcttccctt cgtcattccg gcgaaggccg gaatgacgaa
      721 caagagggtt ggaggagtga cgaggatgga ccggatgtac aacaatcgaa tgctcatgca
      781 agaatgctct tttcccatga cccataaccc attacccata acccggtctt ttaaaggagt
      841 ctaccatgtc ggctgagcgt cttgttgtga tcggcggggt tgccgcgggg atgagcgcgg
      901 cgagcagttt caagagactg aaacccgagg cggaggcgat cgtcctgcag aacgaccatt
      961 tcatctccta cggggcctgc agtctgcctt actacatatc cgacgatgtg aaagatatca
     1021 accgcctcat ctccctgtcc ccggaggtcg cgacgaaaga gaggggcatc accgtgctca
     1081 cccgtcacga ggcgcggtcc atagaccccg ccaggaaaac ggtgaccgcc gtggacctcg
     1141 acgggaacaa ggagaagacc atatcctatg accacctcgt cgtcgcgaca ggaggactgc
     1201 cggtgcgccc tcccttcccg ggaatagacc tcggacacat cttcaccctg aggaccctcc
     1261 acgacggtat cgagatcaag aggttcatag accggtggac aacgttcgaa ccctgcgtcg
     1321 gacctcaatg cgtgtacgtg aaccctttcg gtgccgagaa gagacccatg cgggcctcta
     1381 tcgtcggggg cggctccatc ggcatggaga tgtgcgagtc cctgaggaaa agacgggtcg
     1441 aggtgaccgt cttcgagaag atggaccgcg tcctcggcac catggacacg agcataacga
     1501 acatcgtcga ggaaagactg gcggccgagg gcgtgaacct catgaaaggg gtgtccgtcc
     1561 agagcttcga aggagacagt ggctccgtta cccgcgttat cacggacaag ggaacatttg
     1621 acaccgacat ggtcctgctg gtgatcggcg caaggccgaa cacgaagatc gccgtcgatg
     1681 ccgggatcga gcttggcgcg ggcggggcga taaaggttga tgatcatctg aggaccaaca
     1741 tcccggacat ctatgccgcc ggcgactgtg ccgaggccac gcagatggtc accggcaaga
     1801 aagcctacat ccccctgggc gccaccgcca ccctctatgt gggcaacacc gaacagatcg
     1861 a
//
LOCUS       DAOPOA010000520         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_67429_length_1861_cov_2.058140, whole genome shotgun sequence.
ACCESSION   DAOPOA010000520 DAOPOA010000000
VERSION     DAOPOA010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67429_length_1861_cov_2.058140"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="PKJ17_09965"
     CDS             complement(<1..1838)
                     /locus_tag="PKJ17_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOD76344.1"
                     /translation="MTENDAKLLFSLFGAFTSWSPIVPRKPKELASHLAPFCRLIRDD
                     VIESLKTPRSPMRSLKDEIRNLLFPDADDFRFADAYAQTVIFALLLAHLEKADVLDLR
                     DAYETLYKHHSLLSRSLEFLTDKEARKEISTSLSLVQRVIHATPREAFVAKGDGSDPW
                     LFFYEDFLAAYDPELRKRAGVYYTPLEVVQCQVRLIDEVLQKEFGKEMGFVEAGVATL
                     DPAAGTGTYLLTIIEHALGRVEEEEGPGAVKGGARSLLHNLHGFEWMVGPYAVAQLRF
                     SQALTKHKVALPDAGTGIYLTNTLESPHVKPPVPPLFHKPIAQEHERALKVKDSQHVL
                     VCLGNPPYGRHESAERDGSNRETTGGWVRYGGEEEKPILEDFLRPAREAGYGVHLKNL
                     YNLYVYFIRWAIWKVFEHKTAVGPGVVSFITASSYIDGDAFAGVREHMRRICDHIDII
                     DLGGEGRGTRREENVFAIQTPVAIFIAWRKKVKDHDAPAKVRYVKIEGTREEKLAALG
                     VIVCGKDFRWEAVPDGWQDPFRPESTSDFSKWPNITDIFPWQQSGIEFKRTWPIAPQA
                     EILKERWATLLKSSDRATLMRETGDRTIKQKHLDLLDFNRELPSIES"
BASE COUNT          369 a          567 c          501 g          424 t
ORIGIN      
        1 gattcaatcg agggcaattc tcggttaaaa tccagtagat ccagatgctt ctgttttatt
       61 gtccggtcac cggtttctct catcaatgta gcacgatcac tggatttcaa caaagtagcc
      121 caacgttcct tcagtatttc tgcttgtggc gcaatcggcc acgttctctt aaattcgata
      181 cccgattgtt gccaggggaa aatatctgtt atattaggcc acttgctgaa gtcacttgtg
      241 ctttccggtc tgaacggatc ctgccacccg tcaggaacag cctcccacct gaaatccttc
      301 ccacagacaa tcaccccgag ggcggccagt ttctcctccc tcgtgccctc gatcttcaca
      361 tatctgacct ttgccggggc atcatggtcc tttaccttct ttctccacgc gataaagatc
      421 gcgacggggg tctggatggc aaacacgttc tcttctcttc tcgttcccct gccctctccg
      481 cccaggtcta tgatatcgat atggtcacag atcctgcgca tgtgctcgcg tacgccggcg
      541 aaggcatccc catcgatgta gcttgaagca gtgatgaacg agacaacgcc gggaccgacg
      601 gccgtcttgt gctcaaacac cttccagatc gcccaccgaa tgaagtagac gtacagattg
      661 taaagattct tcaggtggac gccataaccc gcttcccgtg ccggccgcag gaaatcctcc
      721 aggataggct tctcctcctc gccaccgtat ctgacccacc ctcctgtcgt ctccctgttg
      781 ctcccgtccc tctcggcgga ttcgtgccgg ccataggggg gatttccgag acacaccagc
      841 acgtgctgcg aatccttcac tttcaatgca cgctcatgtt cctgggcgat gggtttgtga
      901 aagagcggtg gtacgggcgg cttcacatgc ggtgattcga gggtgttcgt aagatagatg
      961 cccgtgccgg cgtcagggag cgcaaccttg tgcttggtca acgcctgcga gaaacgcagc
     1021 tgcgcgaccg catacggtcc gaccatccat tcaaacccat gaagattgtg gagaagtgaa
     1081 cgggctcccc ctttcacggc tccggggccc tcctcttctt ccacccgtcc cagcgcatgc
     1141 tcgatgatcg taagcaggta cgtccccgtc cccgccgccg ggtcgagtgt ggcaaccccg
     1201 gcctcgacaa accccatctc cttcccgaat tccttctgca ggacctcgtc tatcagcctc
     1261 acctggcact ggacgacttc gaggggtgtg tagtagactc ccgccctctt cctcagctcc
     1321 gggtcgtacg ccgccaggaa gtcctcgtag aagaagagcc agggatccga gccgtcgcct
     1381 ttcgcgacga aggcctcccg cggtgtggca tgaatgaccc gttgcacgag ggaaagggag
     1441 gtgctgatct ccttccttgc ctctttatcg gtgaggaact ccagggagcg tgacagaaga
     1501 gaatggtgct tgtagagcgt ttcgtaggcg tctctcaggt cgagcacgtc cgccttctcg
     1561 aggtgggcaa ggaggagcgc gaatataacc gtctgggcgt aggcgtcggc aaaacggaag
     1621 tcgtccgcat ccgggaacag gaggttgcgt atctcgtctt tcagggaccg catgggtgat
     1681 ctcggggtct taagggactc gatcacgtca tcccggatga gacggcagaa gggggcgaga
     1741 tgcgatgcca gttccttcgg tttccgcggg acaatcggcg accatgacgt gaaggctccg
     1801 aacagtgaga agagcagctt cgcgtcattc tcggtcacag caccctcacc gtcacagcat
     1861 g
//
LOCUS       DAOPOA010000521         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_67454_length_1860_cov_3.158449, whole genome shotgun sequence.
ACCESSION   DAOPOA010000521 DAOPOA010000000
VERSION     DAOPOA010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67454_length_1860_cov_3.158449"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="PKJ17_09970"
     CDS             <1..1765
                     /locus_tag="PKJ17_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD76345.1"
                     /translation="VGLTETSYPVYYRNFPVEKEDPFYDRDYNLCLLCGRCVRVCNDI
                     RLNGTLSFKQRGKLTTIGPAFGRTHLEAGCEFCGACVAVCPTGALSAKTSKWAGKPDE
                     IVESTCPYCPVGCTLDLKVKAGEVVDVSADYDSPTEHGLICVKGRFAIPEYVLSPDRL
                     AQPTILKPEGYDLLPWEGALDEAAAKLKAAGPKGSFIVVSPQLSNEDLFVAQRFAREV
                     LGTEAILSPVMFDLGSDLRPFLDLAVLSESVDTIDTAAGILTIGLDTTYGFTPVGIAA
                     KRAAKRGAVLVTVSTDECNLDMLSEEAVQSDPAKWPDFMEGVMKSMSGNGTRKKAASS
                     FFKKGDRNVLVIGPDAIFSAKRGEIFESALTMRDSLGWKVIVCHPYTNLMGMLAMGAF
                     PGLKPGGMRSDGALKLKDPAAVVDLKQKWKVGYFIGEVPSPQMPRCDYIIYENALPAP
                     SGIDPDLVLPAALFTEAPGTTISCEGKILVFNKASAPFADSKPDWWIMSSIAERMKKG
                     ALKYASLAAIQSEIRKSIPGFSGKDRRVPRKKVAMEGKAAAARNPHGAGRVMTPLETF
                     RGVALHDHVAGMKAIEERRRK"
BASE COUNT          377 a          573 c          577 g          333 t
ORIGIN      
        1 agtggggctc acggagacgt cctacccggt ctattacagg aacttccccg tcgaaaagga
       61 agaccctttc tacgacaggg actacaacct gtgcctcctg tgtggacgct gtgtcagggt
      121 ctgcaacgac atacgcctga acgggaccct gagcttcaag cagcgcggaa agctgaccac
      181 catagggccg gccttcggac gcacccatct cgaggcgggc tgcgagttct gcggggcctg
      241 cgtcgccgtg tgccccacgg gcgcgctgtc ggccaagacc agcaagtggg cgggcaaacc
      301 cgacgagatc gtggaatcca cctgtcccta ctgtcccgtc ggctgtaccc tcgacctcaa
      361 ggtgaaggcg ggagaggttg tcgacgtcag cgccgattac gattcgccga ccgagcacgg
      421 gctcatctgt gtgaaggggc gcttcgcgat ccccgagtac gtcctgagcc ccgacaggct
      481 cgcgcagccg acgatactca agcccgaggg gtacgatctc ttgccctggg agggcgccct
      541 tgacgaagcc gcggcgaagc tcaaggcggc cggcccgaag ggttccttca tcgtcgtctc
      601 accccagctt tccaatgagg accttttcgt ggcccagcgc ttcgcgagag aggtcctggg
      661 gacggaggcg atcctctcgc ccgtcatgtt cgatctcggc agtgacctga ggcctttcct
      721 cgaccttgcc gtgctctcgg aatccgtgga caccatagac acggcggcgg gcatcctgac
      781 catcggcctc gacaccacgt acggattcac ccccgtgggc attgccgcca agagggcggc
      841 caagcggggc gccgtcctgg tcaccgtaag caccgacgaa tgcaatctcg acatgctctc
      901 cgaagaggcc gtccagtccg acccggcaaa gtggcccgac ttcatggagg gtgtcatgaa
      961 gtccatgtcc ggcaatggga cccggaagaa ggcggcatcc tccttcttca agaagggcga
     1021 caggaacgtt ctcgtcatcg ggcccgacgc gatattctcg gccaagcgcg gggagatatt
     1081 tgagagcgcc ctcacgatga gggacagcct cggatggaag gtgatcgtat gccatcccta
     1141 tacgaacctc atgggcatgc ttgccatggg cgcctttccc ggtctgaagc cgggagggat
     1201 gaggtcggac ggggcgctca agctcaagga cccggcggcc gtcgtcgacc tcaaacagaa
     1261 atggaaggtc ggttatttca ttggcgaggt accctctcct cagatgccca ggtgcgatta
     1321 catcatctat gagaacgccc tgcccgcgcc ttcggggatc gaccccgacc ttgtccttcc
     1381 ggcggccctg ttcacggagg ctccggggac aacgataagt tgtgagggca agatcctcgt
     1441 tttcaacaag gcatcggctc cctttgccga ctcaaagcct gactggtgga tcatgtcgtc
     1501 catagcggaa aggatgaaga agggcgccct gaaatacgct tctctcgcgg ccatacaatc
     1561 ggagatcagg aagtccattc ccggcttttc gggcaaggac cgacgggtcc caaggaaaaa
     1621 ggttgccatg gaaggcaagg cggccgcggc acgcaacccc catggggcgg gccgggtgat
     1681 gacccccctt gagaccttcc gcggtgtggc ccttcacgat catgtggccg gcatgaaggc
     1741 gatagaagaa aggaggcgca agtgaagaag gtcatagaac ggtcgatgat cgtccccaat
     1801 atgcatctgc tcgtagtcga ggcacccgag atcgcctcca aggtgaagcc cggacagttc
//
LOCUS       DAOPOA010000522         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_67554_length_1858_cov_2.844149, whole genome shotgun sequence.
ACCESSION   DAOPOA010000522 DAOPOA010000000
VERSION     DAOPOA010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67554_length_1858_cov_2.844149"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..718
                     /locus_tag="PKJ17_09975"
     CDS             134..718
                     /locus_tag="PKJ17_09975"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOD76346.1"
                     /translation="MRRSFLYGFIALVVMMFAGCGYNTIQQNDEAVKAAWGDVEATYQ
                     RRNDLVPNLVETVKAYAKHESETLQAVTEARAKVGSVQVSKDMIGDPQAMARFQQAQA
                     SMGGALSRLLLVAERYPDLKASQNFRDLQNQLEGTENRINVARTRYNKAVQTFNTSIR
                     VFPSNLTNMFILKLPQKEPFKAEAGADKAPKVKF"
     gene            739..1665
                     /locus_tag="PKJ17_09980"
     CDS             739..1665
                     /locus_tag="PKJ17_09980"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOD76347.1"
                     /translation="MKGSRTGTTRRMGHVSGALRRCIAISVAFCLAVLWVAACAHALE
                     VPALKGRVNDYAGMMSPGVRAQLEKELQDFEGSESTQIVVLTIDSLEGESLEEFSIKV
                     AETWKIGQKGKDNGAILLVAKKDRKTRIEVGRGLEGKLTDLTAGRIVQLVINPQFKRG
                     DFDGGFSSGIRAMIDATRGEFKADAARSRSGKRGGPGSTLPFLIFGAIFLLVLGRISR
                     VLGGGAGLIGFSALGFLLGFSLVTVVLLGILGLVLGVFLPFLFGGSRGGGGFGPGGYW
                     TGGGFSSGGDFGGGGFSGGGGDFGGGGASGDW"
     gene            1662..>1858
                     /locus_tag="PKJ17_09985"
     CDS             1662..>1858
                     /locus_tag="PKJ17_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76348.1"
                     /translation="MMKKALNFFTHVEKERIREATSLAESKTLGEIAVMVVDASSRYR
                     EAEVLGAVTFGNVAAFLIAEF"
BASE COUNT          358 a          507 c          622 g          371 t
ORIGIN      
        1 ctcgctatga tggtacccca gtttttgttc ctgacacaaa gaatgataca ttacctcaag
       61 gccggaaggc ctgtattttc ttccccggaa tatggtacta taggctcgat aatctcagga
      121 caggaggaac gtgatgaggc gatcgtttct gtatggtttc attgcactgg tggtgatgat
      181 gttcgcgggt tgcggctaca atacgataca gcagaacgac gaggccgtga aggcggcatg
      241 gggtgacgtc gaggccacgt accagaggcg caacgacctg gtgcccaacc tcgtggagac
      301 ggtgaaggcc tacgcgaagc acgagagcga gacccttcag gccgtgacgg aggcccgggc
      361 aaaggtggga agcgtccagg tgtcgaagga catgatcggc gatccccagg ccatggccag
      421 gttccagcag gcgcaggcgt cgatgggcgg ggccctgtcg cggctccttc tcgttgccga
      481 gcgctatccc gacctcaagg ccagccagaa tttccgcgac cttcagaacc agctggaagg
      541 cacggagaac aggataaacg tggcacgcac gagatacaac aaggccgtgc agacattcaa
      601 cacttccatc cgggtcttcc cgagcaacct gacgaacatg ttcatactca agcttcccca
      661 gaaggagccc ttcaaggcgg aagcgggggc ggataaggcg ccgaaggtga agttctgacc
      721 caaggtaagg ccgggacgat gaaaggatct cgcacaggca ctaccaggcg catggggcac
      781 gtgagcgggg ccctccggag gtgcattgcc atatccgtgg ctttttgtct cgctgttctc
      841 tgggttgctg cctgcgccca cgcactggag gtgccggccc tgaaagggcg cgtgaacgac
      901 tatgccggca tgatgtcgcc cggcgtccgt gcccagctgg agaaggaact ccaggacttc
      961 gaggggtcgg agtccacgca gatcgtggtc ctcaccatcg actccctgga gggggagagc
     1021 ctggaagagt tctccatcaa ggtggccgag acgtggaaga tcggccagaa gggcaaggac
     1081 aacggtgcca tcctcctcgt cgcgaaaaag gaccgcaaga cgcgcatcga ggtcggacgc
     1141 ggtctcgagg ggaagctcac ggacctgacg gcgggacgca tcgttcagct cgtaatcaac
     1201 ccgcaattca aaaggggcga ttttgacggc ggtttttcct cgggtatccg ggccatgata
     1261 gacgcgaccc ggggggagtt caaggctgac gcggccaggt cccggtcggg gaaaaggggc
     1321 ggtccgggat ccactctgcc attcctcatc ttcggcgcga tctttcttct cgtcttggga
     1381 aggatctcgc gcgtcctggg cggcggtgcc gggctgatag gtttctcggc cctggggttc
     1441 cttctgggat tttcgctcgt gactgtcgtt ctcctcggta tcctcggtct tgtgctcggt
     1501 gttttccttc ccttcctctt cgggggttcc cggggagggg gaggcttcgg tccgggcggt
     1561 tactggacgg gcggtggatt ttcgagtgga ggagatttcg gcggcggcgg tttctccgga
     1621 ggcggaggag atttcggcgg cggcggcgcg tcgggggact ggtgatgaag aaagccctca
     1681 atttctttac tcatgtcgag aaggagagga tccgcgaagc gacctcgctc gcggagtcga
     1741 agaccctggg cgagatagcc gtcatggtcg ttgacgcctc gagccggtat cgtgaggccg
     1801 aagtgctcgg tgccgtcacc ttcggaaatg ttgccgcttt cctcatcgcg gagttctt
//
LOCUS       DAOPOA010000523         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_67605_length_1857_cov_2.356271, whole genome shotgun sequence.
ACCESSION   DAOPOA010000523 DAOPOA010000000
VERSION     DAOPOA010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67605_length_1857_cov_2.356271"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..1014)
                     /gene="budA"
                     /locus_tag="PKJ17_09990"
     CDS             complement(241..1014)
                     /gene="budA"
                     /locus_tag="PKJ17_09990"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOD76349.1"
                     /translation="MKCSFKLLLVAGCLLLAAIPVFAGEDTITQVSTIDALMTGVYDG
                     PTTLGELRGKGDFGLGTFAALDGEMVLLDGVFYQVTSTGDVRRPGPDTRTPFAAVTFF
                     TADRTVPLAPGTDFKVFAAETQRNFPTRNSFYAVRITGKFRMVKTRSVPAQQKPYRPL
                     TEIVKAQPVFDFADVTGTMAGFWCPSFVKGVNVPGYHLHFLRADGKRGGHVLDFVVDN
                     ATMEIDDSREFSLILPDDAAFDKANLEPDRTTELKAVEK"
     gene            complement(1030..1365)
                     /locus_tag="PKJ17_09995"
     CDS             complement(1030..1365)
                     /locus_tag="PKJ17_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76350.1"
                     /translation="MIRLRLFGRCRIYHDPVAPVMREPAQIGWTAWFRTIDLVTPRKL
                     KGEELLGRTRGWWTVAPEDVSEAVRPFGRLVVGDGGELMVEFESQEKVDGLIEALEKR
                     FGDQVQLAP"
     gene            complement(1362..1700)
                     /locus_tag="PKJ17_10000"
     CDS             complement(1362..1700)
                     /locus_tag="PKJ17_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76351.1"
                     /translation="MTDDDTWGRDSQVRYMRTVFAAIERAQKEFLRTVGVSPVDPRLR
                     RFREVALALFERSWVAAMRRGVETGDDSAAAMYIFCLARALSMKGVEVPPASLPGNDR
                     LKRLVDEVLT"
BASE COUNT          370 a          612 c          508 g          367 t
ORIGIN      
        1 cgaagcgata gacatctgcc tggcaagcaa gtaagggtcg caggtctcag gtctcaggtc
       61 tcaggtctca ggtctcaaga caggaataac agaaaagggg caagctcaac agcttgcccc
      121 ttcgtaattg gagttcttag tatgttccct tcttgtaacc tcccgacgac gtttcagaag
      181 ctgggtccgg gtctttttgc gcctgggacc tggaacctga gacctgagac atttcttttc
      241 tcacttctcc accgccttga gctcagtggt cctgtcgggt tccaggttgg ctttatcgaa
      301 agcggcgtca tcggggagga tgagggagaa ttcccgggag tcgtcgatct ccatcgtcgc
      361 attgtcgacg acaaagtcaa ggacgtggcc tcccctcttt ccatcggcac ggaggaagtg
      421 caggtggtag ccgggcacgt tgacgccctt cacgaaggaa ggacaccaga atcccgccat
      481 tgtcccggtg acatcggcaa aatcgaagac cggctgcgcc ttgactatct ccgtgagcgg
      541 ccggtatggc ttctgctgtg ccggaacgct gcgggtcttc accattctga atttccctgt
      601 tatcctgacg gcgtagaagc tgttccgcgt cgggaaattc ctctgcgtct ctgcagcaaa
      661 gactttgaag tccgttcccg gcgcgagggg caccgtcctg tccgccgtga agaaggtcac
      721 tgccgcgaaa ggggtcctcg tatccggacc gggtcttctg acatcgcccg tgcttgtgac
      781 ctggtagaag acaccgtcga gaaggaccat ctccccgtcg agcgcggcaa acgttccgag
      841 gccgaaatca cccttcccgc gcaattcacc cagtgtggtc ggaccgtcat acacgcccgt
      901 cataagggcg tctatcgtcg acacctgcgt gatggtgtcc tctccggcaa aaaccgggat
      961 cgccgccagg agcaggcagc ccgcgacaag caacaacttg aaggaacatt tcatcgctcc
     1021 accctctcgt cacggcgcca gctgcacctg gtcgccgaac ctcttttcca gtgcctcaat
     1081 aagcccatcc accttctcct gggattcgaa ctccaccatc aactcgccgc catccccgac
     1141 gacaagccgt ccgaagggtc tgaccgcctc ggagacgtcc tcgggcgcaa cggtccacca
     1201 gccccgggtc cgtcccagga gctcctcccc tttcagcttc cggggggtca caaggtctat
     1261 ggtgcgaaac caggccgtcc aacctatctg tgcgggctcc cgcatgacgg gggccaccgg
     1321 gtcatggtag atcctgcacc tgccgaaaag acgaagacgg atcacgtgag cacctcgtcc
     1381 acgagtctct tcaatctgtc attgcccggc agagacgcgg gagggacctc gacccccttc
     1441 atggacaatg cccgtgccag gcagaagata tacatcgccg cggcagagtc gtctcccgtt
     1501 tccacgccgc gccgcatcgc cgctacccat gacctttcga acaacgccag ggcgacttcg
     1561 cgaaaccggc gaagccgggg atcgacaggc gaaaccccga cggtccgcag aaactctttc
     1621 tgcgccctct cgatcgcggc gaacaccgta cgcatgtaac gtacctggga atcccgtccc
     1681 caggtgtcgt catcggtcat ctctcctccc cttcacgtcc gctccgcccc ccggcatagg
     1741 ggcacggggc atgacggtga cgctcgctgg aactgcaata caataataga accctccgcg
     1801 ggtgtcaacc gtaaagctcc ttaccgggcg gccctgaccg ggcaccctcc gagcttt
//
LOCUS       DAOPOA010000524         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_67882_length_1851_cov_3.012806, whole genome shotgun sequence.
ACCESSION   DAOPOA010000524 DAOPOA010000000
VERSION     DAOPOA010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67882_length_1851_cov_3.012806"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PKJ17_10005"
     CDS             <1..276
                     /locus_tag="PKJ17_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76352.1"
                     /translation="AILCLIFCYLFGFGCVTSNAITMALEPFTRNAGTASALIGSLQM
                     VAGALSSGLLTWLHNGTIIPVVSIMAGTTCIGLILLFGSRPITDETP"
     gene            complement(389..1072)
                     /locus_tag="PKJ17_10010"
     CDS             complement(389..1072)
                     /locus_tag="PKJ17_10010"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HOD76353.1"
                     /translation="MRRYGFQLGLLLLFLAGLVAGYIAWGDLLTFEVVRKHRDALQLC
                     VERHYLFSIALFSLVLISTAFLVPGALVLSILSGFLFGVVRAVILVDVVMTIGAALAF
                     LSSRYFLGNSIQKRFGSQLAAFNREMEKHGPNYLFVLRIVPALPFFLVNYMAGLTRMS
                     LGKFVSATSIGILPGAFVYSYAGQQMATIRTVEEILSPRIVTAFLALSFFAILPVIIR
                     HWRHFRSSR"
     gene            1202..>1851
                     /locus_tag="PKJ17_10015"
     CDS             1202..>1851
                     /locus_tag="PKJ17_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOD76354.1"
                     /translation="MTLLTYNTHSAVGTDGAIAPERIADVLSECDPDIICLQELDIGL
                     RRTGSVDQARYIACQLKMSYHFHPALHIAEGQYGNAVLTRFPMRLVRAGALPGLPQRS
                     RLEKRGAVWVEVLVQDRPVQIVTVHMGLNRRERLAQAVALTGPEWVGAPQCKLPVILC
                     GDLNALPASPVHRLFRANMRDAHLAAGRPQATWPSKCAIARIDYIYVSKDVIVRDCT"
BASE COUNT          418 a          566 c          502 g          365 t
ORIGIN      
        1 gcgatccttt gtctcatctt ctgttacctc ttcggtttcg gatgcgtcac atcgaatgct
       61 ataaccatgg ccctggagcc cttcacgagg aacgcgggaa ccgcctcggc gcttatcgga
      121 agcctccaga tggtggccgg cgccctgtca tcgggactgc tgacctggct gcacaatgga
      181 acgatcatcc cggtggtatc gataatggcc gggaccacat gcatcggcct tatcctgctc
      241 ttcggctcca ggccgatcac cgacgaaacc ccctaggaag tatcccggga aggcctttta
      301 cacttccacg gcgcatcgca ggtgggtcct ccgtcccctt gaccaccaac accaacggca
      361 acccttccgc aggtaatgcg aatcccggtc acctgctcga cctgaaatgc ctccaatgtc
      421 ttatgatgac aggaaggatg gcgaaaaagg acagagccag gaatgccgtc acaattcttg
      481 gcgacaggat ctcttcaacg gtccttattg ttgccatctg ctgcccggca taagagtaaa
      541 caaaggcgcc cggaagaatc ccgatcgacg ttgccgacac gaacttaccg agagacatcc
      601 tggtcagacc agccatgtag tttacaagaa agaacggcag cgccgggacg atcctcagaa
      661 cgaaaaggta gttcgggcca tgtttttcca tctccctgtt gaatgcggca agctgtgacc
      721 cgaatctctt ctggatcgaa tttcccagga agtagcgaga tgacaggaac gcgagcgccg
      781 cgccgatggt cataaccacg tccaccagaa tgaccgccct gacaaccccg aacaggaaac
      841 cgctcaggat ggacaatacg agcgctccag gaacaaggaa cgccgttgat atcaggacga
      901 gggagaacaa cgcgatcgag aacaggtaat ggcgctcgac acacaattgg agagcatcgc
      961 ggtgcttcct taccacctca aaggtcagca gatcgcccca cgcgatgtac cccgcaacga
     1021 ggccggccag aaagagaaga agaagcccga gttgaaatcc gtatcgtctc atgagatcct
     1081 gtttgccgtc atcaaactgc ctccggcgtg agagcagccg tatacttgcc tttcggcccc
     1141 tccctcttga aagagccgct ttcactcgtt agattctgtc tgtatgagag aacgccagag
     1201 tttgacgctt ctgacctata atacgcacag cgccgttgga accgacgggg cgatcgcgcc
     1261 cgaaaggatc gcggatgtgc tatcggagtg cgatcccgat ataatctgcc ttcaggagct
     1321 ggacatcgga ctgagacgaa cgggctcggt ggaccaggcg cggtacatcg cctgccagct
     1381 caagatgtct tatcactttc accctgcgct tcatatcgcg gaaggccagt atggaaacgc
     1441 ggttctgacc cggtttccca tgcgtctcgt tcgcgccggc gctctcccgg ggctgcctca
     1501 acgctcaagg ctcgagaaaa gaggggccgt ctgggtcgag gtgctcgttc aggaccgccc
     1561 ggttcagatc gtgactgtcc acatgggtct caaccgccgc gaacgcctcg cgcaggcagt
     1621 cgctctcacg ggtcccgaat gggtaggagc ccctcagtgc aaacttcccg tcatactctg
     1681 cggcgatctc aatgccctgc ccgcctcccc cgtgcaccgc ctgttcaggg ccaacatgag
     1741 agatgcgcac ctcgctgcgg gacgccccca ggccacgtgg ccgagtaaat gcgcgattgc
     1801 acggatcgat tacatctatg tctcgaagga tgttattgtg cgcgattgca c
//
LOCUS       DAOPOA010000525         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_67895_length_1851_cov_2.500000, whole genome shotgun sequence.
ACCESSION   DAOPOA010000525 DAOPOA010000000
VERSION     DAOPOA010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67895_length_1851_cov_2.500000"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PKJ17_10020"
     CDS             <1..729
                     /locus_tag="PKJ17_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD76355.1"
                     /translation="NLLESELFGYRKGAFTDAKIDKEGLVVGAAGGTLFLDEVTEMPP
                     SLQAKILRVIEEREVRPLGGTTSYPTDVRIISTSNRDIQSRIDQGLFRSDLYYRLRVI
                     DLDLPPLRGRKEDIPLLVRHFLNTFNKELKKKVTHVSTEAMRILMDYSWPGNVRELEN
                     VVQRAVTLCRGETVVPDDFPALMTRTNGRSFLDRGPEKGRTLEELETEYIREILAEVG
                     GNRSRAAEILGIDRKTLYRKLKNE"
     gene            995..1237
                     /locus_tag="PKJ17_10025"
     CDS             995..1237
                     /locus_tag="PKJ17_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76356.1"
                     /translation="MARKLSARRRIPKSKFDAQETHICPRCNAFLVRERFYGMYAHIW
                     VWRCIKCGEIIDDVVLYNRRLLKKAAKGVDIMTLIR"
     gene            1473..>1851
                     /locus_tag="PKJ17_10030"
     CDS             1473..>1851
                     /locus_tag="PKJ17_10030"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOD76357.1"
                     /translation="MMERSGFAVWFTGLPSSGKTVTAEKTCELLYERGVPMVFFDSEE
                     LRRTLIQRPVYTDEERDLFYSVVVYLVECITERGVNVAISATTPRRYYRATARLHIEK
                     FAEVFVDCPEALCKTESVCAEVSE"
BASE COUNT          425 a          505 c          568 g          353 t
ORIGIN      
        1 aacctgctgg aaagcgagct cttcggatac cggaagggag ccttcacgga cgcgaagatc
       61 gacaaggagg gactcgtcgt gggggccgcc ggcggcaccc ttttcctcga cgaggtcacg
      121 gagatgcccc cctcgcttca ggccaagata ttaagggtca tcgaggagag ggaagtgcgg
      181 ccgctgggag gcacgacctc ttatcccacg gacgtccgca taatctccac gtccaaccgc
      241 gacatccagt cacggataga ccaggggctg ttccgaagcg atctttatta ccggctccgt
      301 gtcatcgacc tcgatcttcc tccgctcagg gggagaaaag aggacattcc tcttctcgtc
      361 aggcacttcc tgaacacatt caacaaggag ctgaaaaaga aggtcaccca tgtctccacg
      421 gaggcaatga ggatcctgat ggactattca tggccgggta acgtgaggga gctcgagaac
      481 gtggtccaga gggccgtcac gctgtgccgc ggtgagaccg tcgtccccga tgatttcccc
      541 gcgctcatga caaggacgaa cggcaggtcc ttccttgacc gcggtccgga aaagggccgg
      601 accctggagg agctcgagac ggaatacatc cgggagatac tggccgaggt cgggggtaat
      661 cgctcccggg ccgcggagat actcggcata gacagaaaga cgctctatcg caaactcaag
      721 aacgaataag cggagatccc cggtgcccgg gagcactggg gcacaatgcc tgcccgatgg
      781 ggaccttttg ccccattacc cgaaagacat cccggtttcg gccggacatt ccccttcatc
      841 gcaatagact atctggcgtt catttgcgcc tgttttcgac gccatggcgt gcaaattgca
      901 gactcgcggc aagttgcata ttccgggtga gtgtcccagc gaagctgcaa catccttaga
      961 aaattgatgg ctccacagca aggaaggagg aaccatggca aggaaactaa gcgcacgcag
     1021 gcgaataccg aagagcaagt tcgatgccca ggaaacacac atctgtccgc ggtgcaatgc
     1081 ctttctggtc cgcgaaaggt tctacggcat gtacgcccac atctgggttt ggcgatgcat
     1141 aaaatgcggc gagatcatag acgacgtggt gctctacaac cggcgactgc tcaagaaggc
     1201 cgccaagggg gtagacataa tgacgctgat ccggtgacac gggatcggca agggcgtacc
     1261 atgcgtcacg cggagcttgt gttttccggg taaaccatcg tgggtgtgat caaaaccagc
     1321 gcgtttcaag agggggcggt actgccggag ttcccgtctt gcgagagggc gggccgcacc
     1381 cctcgtcact ttcatccaag ctcagcgctc agttcgttcc cgggtcggaa cgaaatcggg
     1441 ttgacaaatg aagacacaga acagggggtg ggatgatgga gcggtcggga ttcgcggtgt
     1501 ggttcaccgg gttgccgtca tcgggtaaaa cggtgacagc ggaaaagacc tgtgagctgc
     1561 tgtacgaacg tggcgtgcct atggtgttct tcgattccga agagcttcga aggacgctga
     1621 tccagcgccc tgtctatacc gacgaggaga gggacctgtt ctacagcgtt gtggtctatc
     1681 tcgtggagtg tatcacggaa aggggggtca acgtggccat atcagccacg acgccgcggc
     1741 ggtattatcg ggcaacggcc aggcttcaca tcgaaaagtt cgccgaagtc ttcgtggact
     1801 gtcccgaagc tctttgcaaa acggaatccg tctgtgcgga agtctccgaa g
//
LOCUS       DAOPOA010000526         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_68048_length_1848_cov_2.494144, whole genome shotgun sequence.
ACCESSION   DAOPOA010000526 DAOPOA010000000
VERSION     DAOPOA010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68048_length_1848_cov_2.494144"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="PKJ17_10035"
     CDS             complement(<1..1449)
                     /locus_tag="PKJ17_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOD76358.1"
                     /translation="MKVITSHMNADFDSLSSMVAARKLYPDATLVFPGSQEKTLRDFL
                     IHSTLYLFDIAKLKEIDHRSVDTLVLVDTRDKTRIGDLAKVADDPKVAIHVYDHHPDS
                     GNDVRADVQVVRSVGATVTILVSMIRERGISLTPEEATIMMLGIYEETGSFRFSSTTV
                     EDFEAASYLLSQGANVNLVSDMLVRELTPEQVFLLNDIIKNATVYNINGIDIVITEGS
                     TEQYVGDLAVIVHKYRDMENINAVFALFRMEDRIHIIGRSRIPEVDSGYVMSLLGGGG
                     HKVAASSTVKEMTLPEAKERLIEILRNNVKPLWKAKDIMFFPVKSVDSQSAIGEALSV
                     LTKYNINAMPVLSRGKVVGVITRQVAAKALFHKLAEQPVDDYMFTEFQSVGPEDSIEA
                     VKEKIIGTNQRFLPVVEKNELKGAITRTDLLRVLEDEIAKTVLEKLEFHEKYVQRKNV
                     RKLMEERLDEATMEKLVNMGNLADEMGFHAYLV"
     gene            complement(1449..1814)
                     /locus_tag="PKJ17_10040"
     CDS             complement(1449..1814)
                     /locus_tag="PKJ17_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOD76359.1"
                     /translation="MGKRSKERVVPLGSYSRDAIRAYLDAERPRGQYLFPNRRGGMMT
                     RQAVWKIIRKYGLKLSRGKVSPHTIRHTFATHLLEGGADLRSVQVLLGHEDISTTQIY
                     THVDRKRLKEIHKKHHPRG"
BASE COUNT          342 a          549 c          532 g          425 t
ORIGIN      
        1 cacgagatag gcgtgaaaac ccatctcgtc ggcaaggttg cccatgttca cgagcttttc
       61 cattgtcgcc tcatccagtc gctcctccat gagcttgcgg acgttcttgc gctgcacgta
      121 cttttcgtgg aactcgagtt tttccagaac ggtcttggcg atctcgtcct cgaggaccct
      181 caggaggtcc gtcctcgtga tggccccctt gagttcattc ttctccacca cgggaaggaa
      241 ccgctggttg gtgccgatga tcttctcctt caccgcctcg atggagtcct cgggacccac
      301 ggactggaac tcggtgaaca tgtagtcgtc gacgggctgt tccgccagct tgtggaacag
      361 tgccttcgcc gccacctgcc gcgtgatgac ccccaccacc ttgccgcgtg aaagcacggg
      421 catggcgttt atgttgtact tcgtaaggac gctcagggct tcgccaatgg cgctttggga
      481 atcgacggac ttgacgggga agaacatgat gtcctttgcc ttccagagag gcttgacgtt
      541 gttgcgaagg atctcgatga gcctttcctt cgcctcggga agcgtcatct cctttaccgt
      601 ggacgacgct gcgaccttgt ggcccccgcc gccgagaagg gacatgacgt accccgaatc
      661 cacctccggt atcctgctgc gcccgatgat gtggatccgg tcctccatcc ggaacagggc
      721 aaagacggca ttgatgttct ccatgtccct gtacttgtgg acgattacgg caaggtcccc
      781 cacgtactgc tccgtgcttc cctcggtgat gacgatatcg atgccgttga tattatagac
      841 cgtggcattc ttgatgatgt cattgagaag gaagacctgc tccggagtca gctcccggac
      901 aagcatgtcg gatacgagat tgacgttcgc tccctgggaa aggagatagg acgcggcctc
      961 gaagtcctcg acggtggtgg aggaaaacct gaaactgccc gtctcctcat atatgcccag
     1021 catcattatc gtcgcctcct cgggcgtgag acttatgccc ctctccctta tcatggagac
     1081 aaggatggtc accgtcgcac cgacgctcct gaccacctgc acatccgccc tcacatcgtt
     1141 gcccgaatcg ggatggtggt catatacgtg tatcgcgacc ttcggatcgt ccgccacctt
     1201 cgccaggtcg ccgatgcgcg tcttgtcccg cgtgtcgaca aggacgaggg tgtcgacgga
     1261 acgatggtct atctccttga gcttggctat gtcaaagaga tagagcgtcg agtggatgag
     1321 aaaatcgcga agcgtcttct cctgggaccc cgggaacacg agggtcgcgt cggggtagag
     1381 tttcctcgcc gccaccatcg aggagagaga atcgaaatcc gcgttcatat gggacgtgat
     1441 gaccttcatt aacccctcgg gtggtgtttc ttgtggatct ccttcaggcg cttgcggtcg
     1501 acgtgggtgt atatctgtgt tgtcgatatg tcctcgtgtc ccaggagcac ctggaccgac
     1561 cgcaggtccg cgccgccctc aagcaggtgt gtggcgaagg tatgccgtat ggtatgaggc
     1621 gacaccttgc cccgtgacag cttgaggccg tacttccgta tgatcttcca gacggcctga
     1681 cgggtcatca taccgccgcg gcggttcgga aaaaggtact ggccccgggg tctttccgcg
     1741 tcgaggtagg ccctgatggc gtccctcgaa taggagccga gcggcacgac ccgttccttg
     1801 gagcgctttc ccatggcgac gacgaacccg ccatccaggt tgacatcg
//
LOCUS       DAOPOA010000527         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_68084_length_1847_cov_3.181920, whole genome shotgun sequence.
ACCESSION   DAOPOA010000527 DAOPOA010000000
VERSION     DAOPOA010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68084_length_1847_cov_3.181920"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PKJ17_10045"
     CDS             complement(<1..504)
                     /locus_tag="PKJ17_10045"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOD76360.1"
                     /translation="MKRSLHMPYDATKMADWQISEAAEEKMKSVRQLSDEMGLGDNEV
                     IPYGKLGRLNYAAIMQRLREKSNGKYIDVTAITPTPLGEGKTTTTIGLLEGLGKRGRN
                     VGGAIRQPSTGPTMNVKGSAGGGGNAQVVPLTEFSLGLTGDIDRIMNAHNIAMVALTA
                     RMQHERNY"
     gene            702..1184
                     /locus_tag="PKJ17_10050"
     CDS             702..1184
                     /locus_tag="PKJ17_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76361.1"
                     /translation="MDLIASGDLKGILDVLSFMADVERALAELYHACGETWDDGHGFW
                     SGIEDEENKHARNIESMKDIISRKPERFEKGRPFNIMAAQTLIRGIRGNIDKVRTGTI
                     QRKNALFMARDMEQSMMEQRYGEVVKTEDIEYLGLMKEIISDTAAHKAKLDAEIRNLN
                     "
     gene            complement(1300..>1847)
                     /locus_tag="PKJ17_10055"
     CDS             complement(1300..>1847)
                     /locus_tag="PKJ17_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76362.1"
                     /translation="QMDEMERTGTPLTYTDEIWIRNGERLNQKQRHRKYSGEIFEHCL
                     PLCIISPSSAVIRRSVFDAAGVFDESLPVCEDYDMWLRITARFPVTFIQKHLIIKRGG
                     HPDQLSRAYDGMDRFRIASIVKVLDSGVLSPGQTRKAIGELRTKCRIYAGGAMKRGKK
                     EEGERYLRLASAYEDEATQGE"
BASE COUNT          369 a          537 c          523 g          418 t
ORIGIN      
        1 gtagttgcgc tcgtgctgca tccgggccgt gagagcgacc atggcgatgt tgtgggcgtt
       61 catgatgcgg tctatatcgc cggtcagacc cagggagaac tccgtcagcg gcacgacctg
      121 cgcgttgcct ccgcctcccg cgctcccctt gacgttcatc gtcggacccg tcgacggctg
      181 gcgaatggcc cctcccacgt tcctgcctct tttgcccagt ccctcgagaa ggccgatcgt
      241 tgtcgttgtc tttccttcac cgagcggcgt gggggtgatg gccgtgacgt ctatgtactt
      301 cccgttcgac ttctcccgca ggcgctgcat gatcgcggca taattgaggc ggcccagctt
      361 cccgtaaggt atgacctcgt tgtccccaag acccatctca tcgctgagtt gccggacact
      421 cttcatcttc tcctcggcgg cctcggatat ctgccagtcc gccatctttg tcgcgtcata
      481 gggcatatgc aggctccttt tcatgagaga ggcttgaact tcgcctcaaa gcattgataa
      541 atatacaggt ttcggcacga tttctcaagc ccggcgacgc gatgtcgcgt ctttcggatt
      601 gaatatgtcc tccgcttctg atagttttta gggcggcatc acggtgtaat gatgcctcag
      661 aaggtttcgg ggaactcacc cggcgatatg acgaggtgga tatggatctg atagcatcgg
      721 gagacctgaa aggtatcctc gatgtcctgt cctttatggc agacgtggaa agggcgctcg
      781 ccgagcttta tcatgcctgc ggagagacct gggacgacgg gcatggtttc tggtccggca
      841 tcgaggatga agagaacaag cacgcacgca atatcgagag catgaaggac atcatatccc
      901 gaaaaccgga acgcttcgag aaaggccgtc ccttcaatat aatggctgcc cagaccctca
      961 tcagagggat cagggggaac atcgacaagg tgaggaccgg caccatccag agaaagaacg
     1021 ccctcttcat ggcccgtgac atggaacagt ccatgatgga acagcgctac ggtgaggtcg
     1081 tcaagaccga agacatcgag tacctcggtc tcatgaagga gataatctcc gataccgccg
     1141 cgcacaaggc caaattggac gccgagatca ggaacctgaa ctgaaagctg tgctccaagc
     1201 aggcgggcgc ttcgcgtcaa cggagcgacg ggtccggtgc ttcgttcgca cacggagaca
     1261 cctcgcggga tagcccgaga ggacgatacg atcgttcatc tattccccct gggtcgcttc
     1321 gtcttcatat gccgatgcca gtctgagata gcgctcccct tcttccttct tgcctctttt
     1381 catggcaccg cccgcataga tccggcattt tgtccggagt tctccgatcg ccttccgggt
     1441 ctgtcccgga gacaggacac cgctgtcgag gacctttacg atgctcgcta tcctgaagcg
     1501 gtccatcccg tcataggccc gtgaaagctg gtcgggatgc cctccccgct tgatgatgag
     1561 atgtttttgg ataaaggtca cgggaaatcg cgcggttatc ctgagccaca tgtcatagtc
     1621 ctcgcacacg ggcagggatt catcgaaaac accggcagca tcgaagacgc ttcgtcggat
     1681 gaccgccgac gacgggctga tgatgcaaag gggcaggcaa tgttcgaaga tctcccccga
     1741 gtacttgcgg tgccgctgtt tctggttgag cctctccccg ttgcgtatcc atatctcgtc
     1801 cgtgtaggtg agaggtgtgc ccgtgcgctc catttcgtcc atctgga
//
LOCUS       DAOPOA010000528         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_68177_length_1845_cov_3.111732, whole genome shotgun sequence.
ACCESSION   DAOPOA010000528 DAOPOA010000000
VERSION     DAOPOA010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68177_length_1845_cov_3.111732"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PKJ17_10060"
     CDS             <1..853
                     /locus_tag="PKJ17_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOD76363.1"
                     /translation="TSDPDIYAVGDCVEVTNLITGKKVLAPYGDLANLQGRVAGENAI
                     LGDHATFPGTIQTGICKVFDYTAGSTGLSERAARESGFNEIETAINASPDKPGFMEGK
                     ILVTKIVVDKPSGRILGAQCVGLGDVSKQIAQWAMAIKGRLTVDDMVNADLPYAPPFS
                     LAIDHGIASAHIVQNKLRGFMKGISCTEVKEKIDRKEKVFFLDTRGPDEYEQMRLGIG
                     ETLIPIGMLRKRLDEIPQDRDTEIVCYCKISLRGYEAARLLESQGWKNVRVMEGGIMA
                     WPFPREK"
     gene            complement(1019..>1845)
                     /locus_tag="PKJ17_10065"
     CDS             complement(1019..>1845)
                     /locus_tag="PKJ17_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOD76364.1"
                     /translation="YFNPAGYVTHAVVASEDITDFKKEEESMEKSDPGFRALLRNMHY
                     GVFTFDLEGRFTFVNDVIVKRSGYPREWYVGKSLFDVVRPQERDEVRKHFKATARGDQ
                     VAPYEFSFVKAADEIAWAHISTTPIKEGGQIVGVLGVMLDITKRVQSERALVESEEKY
                     RRLFEESRDAIFITDNEGRLTDVNRAFLKLFGYKKEEIEGLRAIDTYLNREECLTYMK
                     EVEQEGFVEDFVAKLKKKDGTVMECLLNGTPLRGVDGTIRGYQGIARQETGRMQPR"
BASE COUNT          391 a          565 c          525 g          364 t
ORIGIN      
        1 gacctccgat cccgatatct atgccgtggg cgactgcgtg gaggtcacga acctcatcac
       61 cggcaagaag gtcctggccc cttacggtga ccttgcaaac ctgcagggac gggtggcggg
      121 ggagaacgcg atactcggcg accacgcgac cttcccgggg acgatccaga ccggcatctg
      181 caaggtcttc gactacacgg cgggttcgac gggtctgtcg gagagggcgg cccgggaatc
      241 gggattcaac gagatagaga ccgcgatcaa cgcgagccct gataaacctg gtttcatgga
      301 gggaaagata ctcgtcacaa agatcgtcgt cgacaagccc tcgggcagga tcctcggcgc
      361 gcagtgcgtc ggcctgggcg acgtgagcaa gcagatcgcc cagtgggcga tggcgatcaa
      421 gggcaggctg acggtggacg acatggtcaa cgccgacctg ccttacgcgc cgccgttctc
      481 ccttgccata gaccacggga tcgcctcggc acacatcgtg cagaacaaac tcaggggatt
      541 catgaaggga atatcgtgca ccgaggtaaa ggagaagatc gacaggaagg aaaaggtctt
      601 cttcctcgac acgcggggcc ccgatgagta cgaacaaatg cggctcggca taggagagac
      661 gctgataccg atcgggatgt tgagaaaacg tctcgacgag ataccccagg acagggacac
      721 cgagatcgtc tgctactgca agatatccct gcgcggctac gaggcggccc gcctgctgga
      781 gtcccagggt tggaagaacg tcagggtgat ggagggcggc atcatggcct ggcctttccc
      841 gcgggagaaa tgaggcgggg cggccccgcc gccgcgaaag acccaacctg cgcaccgttc
      901 atcctggcgc ggacaccacg ggagaaaaaa agggtaagaa tgaaaatgga tcaaggaaag
      961 caggacgttt ggagggccct gcgcaagcgg ccctccaaca tttgaacctg atatcccgtt
     1021 atctcggctg catcctgcct gtttcctgcc tcgcgatgcc ctgatagccc ctgatcgtcc
     1081 cgtcaacacc acgcaggggc gttccgttca gaaggcattc catcaccgtt ccatccttct
     1141 tcttcagttt cgcgacaaag tcctcgacga agccctcctg ctcgacctct ttcatatagg
     1201 tcaggcattc ctccctgttc aggtatgtgt cgatggcccg caacccttcg atctcttcct
     1261 tcttgtagcc gaagagcttc aggaaggccc ggttcacatc ggtaagcctt ccctcgttgt
     1321 cggtaatgaa tatggcatcc ctcgattcct cgaaaagcct ccggtacttc tcttcgcttt
     1381 cgacgagcgc ccgttccgat tggacacgct tggtgatgtc gagcatcacg ccgaggacgc
     1441 ccacgatctg tccgccttct tttatgggtg tcgtgctgat atgggcccag gcgatctcat
     1501 cggcggcctt gacgaaggag aactcgtagg gcgccacctg gtcaccgcgc gccgtcgcct
     1561 tgaaatgctt gcgcacctca tccctctcct gcggacgcac cacgtcaaaa aggcttttcc
     1621 ccacgtacca ctcccgcggg tatcccgacc ttttcactat cacgtcgttg acgaaggtga
     1681 accgtccttc gaggtcaaag gtgaagaccc cgtaatgcat gttcctcaaa agagccctga
     1741 accccggatc gctcttctcc atggactcct cttccttctt gaaatccgtg atgtcctcgg
     1801 acgccaccac cgcatgggtc acatatcctg ccgggttgaa gtacg
//
LOCUS       DAOPOA010000529         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_68339_length_1842_cov_2.730274, whole genome shotgun sequence.
ACCESSION   DAOPOA010000529 DAOPOA010000000
VERSION     DAOPOA010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68339_length_1842_cov_2.730274"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PKJ17_10070"
     CDS             complement(<1..666)
                     /locus_tag="PKJ17_10070"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOD76365.1"
                     /translation="MRRTNGPENEVAVLITDIHQYSRLTSNMTAEGIRDYMIGYHGHL
                     QTMVMDNQERADEFEPCAGDASIAIFRQKAGEGREQKCRRALKVALTVAEAIESGKIP
                     FTRIGIYVGDIIEAPFGALTLRFGNCFAAANRLEELCAYFGTTILMDREVALAQDDLA
                     QYIVAIGKVTPKSFEHPIHVYSIYKPGIHKCPADVDWERLRAFIALKNEAIEYFIGNA
                     QKGI"
     gene            complement(885..1520)
                     /locus_tag="PKJ17_10075"
     CDS             complement(885..1520)
                     /locus_tag="PKJ17_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD76366.1"
                     /translation="MRNWIVVATIAVLVSAGLMGMGCDKLPWNKPQQKAEPQQPAPPP
                     APEKPAAPAEAAKPASDEPSQEVKAHLKQGMSYVSIAKNATSKSVFNENLDNAIAEFS
                     NAINKDPNYAEAYSNRAVAYMLQKKNNKALDDLKKAKEIKPDSATIRYNLASVHSLMG
                     NTDYGLDELDAALSKGFNDYDSLRKDPDINNLRKSKEFQKILEKHKVFITK"
     gene            complement(1547..>1842)
                     /locus_tag="PKJ17_10080"
     CDS             complement(1547..>1842)
                     /locus_tag="PKJ17_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76367.1"
                     /translation="ASGTGKSLDQDGYGKVLLSASSAGEKSWESDSLKNSVFTYYFVD
                     GLKRYNGSVKDAFTYSKPLVSQRVKQEKGADISQTPQAMATNSNWNMSIKKTP"
BASE COUNT          319 a          481 c          532 g          510 t
ORIGIN      
        1 tatccccttc tgcgcgtttc cgatgaagta ctcgatcgcc tcgttcttca gagcaatgaa
       61 ggcgcggaga cgttcccagt caacatctgc cggacacttg tgtatccccg gcttgtagat
      121 ggaatagacg tggatggggt gttcgaagct cttcggcgtt acctttccta tcgcgacgat
      181 gtattgtgcc agatcgtcct gggcaagtgc tacctcacgg tccatcagga tggttgttcc
      241 gaagtaggcg cagagttcct cgagcctgtt ggcggcggcg aaacagtttc cgaacctgag
      301 ggtaagcgcg ccgaatggtg cctctataat atccccgacg tagataccga tccgggtgaa
      361 ggggatcttt cccgattcga tggcctcggc gacggtaagg gccactttaa gtgcccgtct
      421 gcatttctgt tcccggccct cgcccgcttt ttgcctgaag atcgctatcg aagcgtctcc
      481 cgcgcagggt tcgaattcgt cggccctttc ctggttgtcc atgaccatgg tttgcaggtg
      541 tccgtgatag ccgatcatgt aatcccgaat accttccgcg gtcatgttcg acgtgagcct
      601 ggaatattga tggatgtccg ttatgaggac ggcgacttcg ttttcgggac cgtttgttct
      661 tctcatgctg gtggtgaatc aattatacgc aagaagctgc ctggtagtca aggagcgcaa
      721 aagaagagaa aagcaggtga tgggttatgg gcgatgggtc atgggaagga cacattcttc
      781 tggacacctg ttacccataa cccatgaccc gtccttgaac cagaagcccc gcggatctcg
      841 ggatccacgg ggcttgcagg aagcttgctc tgtggttgca gctgttattt cgttataaag
      901 accttgtgct tttccaggat cttttggaac tccttgctct tcctgaggtt gttgatgtcg
      961 gggtccttcc tcaagctgtc gtagtcgttg aagcccttgg agagggcggc atcgagctcg
     1021 tccagaccgt agtcggtgtt tcccatgagt gaatggacgg acgcgagatt ataccggatg
     1081 gtggcgctgt ccggtttgat ctccttggcc ttcttgaggt cgtcgagggc cttgttgttc
     1141 ttcttctgaa gcatgtaggc aacggccctg ttcgaatacg cctcggcata gttcggatcc
     1201 ttgtttatcg cgttggaaaa ctcggcgatc gcgttgtcca ggttttcgtt gaatacgctt
     1261 ttcgaggtgg cgttcttggc gatgctgacg tagctcatgc cctgtttcag gtgtgccttg
     1321 acctcctggc tcggttcatc cgaggcaggc tttgcggctt cggcgggagc agcgggtttc
     1381 tccggggccg gcgggggtgc cggctgctgc ggttcggcct tctgctgtgg cttgttccag
     1441 ggcagtttgt cacatcccat ccccatgagt cctgcggaaa cgaggacagc tatggtggcg
     1501 actacgatcc aattcctcat ctatcgatcc tcctccttat ttgatatcag ggcgtcttct
     1561 tgatgctcat gttccagttg ctgtttgtgg ccatggcctg cggcgtttga ctgatgtctg
     1621 cgcctttttc ctgcttgacc ctctgggaga caagaggctt tgaataggtg aaggcgtcct
     1681 ttacagagcc gttgtacctc ttcaatccgt cgacaaaata atacgtgaag acactgttct
     1741 tgaggctgtc cgattcccag gatttctcgc cggcgctgct cgctgagagg agcaccttgc
     1801 cgtaaccgtc ctgatcgagg ctcttgccgg ttcctgaggc ct
//
LOCUS       DAOPOA010000530         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_68448_length_1840_cov_2.522129, whole genome shotgun sequence.
ACCESSION   DAOPOA010000530 DAOPOA010000000
VERSION     DAOPOA010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68448_length_1840_cov_2.522129"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="PKJ17_10085"
     CDS             complement(<1..1068)
                     /locus_tag="PKJ17_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76368.1"
                     /translation="MEEKRMATAYVSGILTCEGPLAQLEGRDERSEKTLLLKHRTDSY
                     VVQKRAWVEGTGEEDKGHFETVEGEYMVANILAPSSNTLRAKIRDLLSSDISLKVGAL
                     GPKTLFFMCGGGGGFDEKASKGATIQLVRNIREKNVVAGLLGGSIGGAIIPGKVVVGI
                     PVLYCRDLVNYLPRGVRGKDGTEMPTDPAALPEAWNFLDKKQYTRHDDFNIPEVAEML
                     NEAGRDMLAKEEYSKGGKEEGKKKDVKRQAIYTIEAVAAGAQYWSHFFLRKPLDRELG
                     GFLKALKLFSKDPYIGGLSSKGFGEISLAYDVVVNYTDGSYGKGRVLVNTDRAKGFEV
                     REDGDGVIASAMDSYEKWLGSV"
     gene            complement(1203..1292)
                     /locus_tag="PKJ17_10090"
     tRNA            complement(1203..1292)
                     /locus_tag="PKJ17_10090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1256..1258),aa:Ser,seq:tga)
     gene            complement(1300..>1840)
                     /locus_tag="PKJ17_10095"
     CDS             complement(1300..>1840)
                     /locus_tag="PKJ17_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOD76369.1"
                     /translation="ALDLRDSRGAGPVLRPEKVVESTYGKGLKIEMLQGKSPSGRMLV
                     LAKTKLFTLSPVVLKGKGEHLRLSETAFSPEGDPAINSYILGNLLVKHMNTVNGGSNV
                     PLEMLMEDRSLTVSFSRGAREGWTKLAGSVVPFIEGRFTLTTHPELLYTALNNGIGAR
                     TSLGFGMVEMHNGRKYSGE"
BASE COUNT          311 a          595 c          507 g          427 t
ORIGIN      
        1 gacggagccc agccacttct cgtagctgtc cattgccgac gctatgacgc cgtcaccgtc
       61 ctcccgcacc tcgaagccct tggcgcggtc cgtgttcacg aggaccctgc ccttgccgta
      121 tgacccgtct gtataattga cgaccacgtc gtaggcgaga ctgatctcgc cgaatccctt
      181 gctcgagagg ccgccgatgt acgggtcctt cgagaatagc ttcagggcct tgagaaaccc
      241 gcccagttcc ctgtcgagcg gtttgcgcag gaagaagtgc gaccagtact gcgcgcctgc
      301 ggccacggcc tcgatggtgt agatggcctg gcgtttcaca tctttcttct tcccttcctc
      361 cttcccgccc ttggagtatt cctcctttgc aagcatatcc ctgccggctt cgttgagcat
      421 ctcagcaact tccgggatgt tgaaatcatc gtgccgggtg tactgcttct tgtccagaaa
      481 attccaggcc tcgggaagcg ccgcggggtc cgtcggcatt tccgtaccgt ccttccccct
      541 gacgcccctg ggcaggtagt tgaccagatc ccggcagtag aggacgggga tcccgacgac
      601 caccttgccg gggatgatcg caccgccgat ggagccaccg aggagaccgg ccacgacgtt
      661 cttctcgcgg atgtttctta cgagctgtat cgtggcgccc ttcgacgctt tctcgtcgaa
      721 cccgccgcct ccgccacaca taaagaacaa ggtcttggga cccagcgcac cgaccttcag
      781 cgagatatcc gatgacagca gatcgcggat cttcgccctc agggtattac tgctcggcgc
      841 gaggatgttc gcgaccatgt attcaccctc gactgtctcg aagtgccctt tgtcttcctc
      901 accggtgcct tccacccagg cgcgtttctg cacgacgtac gaatccgtcc tgtgtttgag
      961 gaggagtgtc ttctcgcttc tctcgtccct gccctcgagc tgcgccaggg gcccctcaca
     1021 ggtcaggata ccgctcacat acgccgttgc catccttttc tcttccatga ttccctcctt
     1081 ttgggatcgt attttctatg tttacagcgc catgcggggg actaagcccc ctttgcatgg
     1141 cggccgtcca gcaggacgga cttcagcccg atgccgaagc agagcgggta ctgctctttt
     1201 tgtggcggag agagcaggat ttgaacctgc ggggcgtttg cacgcccagc gattttcagg
     1261 accgccgctt tcagccgctc agccatctct ccgtcgaact tattcgccac tgtatttcct
     1321 gccgttgtgc atctcgacca tcccgaaacc gagagacgtc ctggcgccta tgccgttgtt
     1381 cagggccgtg tacaggagtt cgggatgtgt agtcagggtg aagcggccct cgatgaaggg
     1441 gaccactgac ccggccagct tcgtccaccc ttccctcgct ccgcgggaga atgacaccgt
     1501 cagcgacctg tcctccataa gcatttccag gggcacgttc gaaccgccgt tcaccgtgtt
     1561 catgtgcttg accaggagat tacccagaat gtagctgttg atggcgggat cgccctcagg
     1621 ggagaaggct gtttctgaca gcctgagatg ttcgcctttc cctttgagta cgaccggcga
     1681 cagggtaaag agtttggttt tcgccaggac aagcatccgc ccggaggggc tcttcccctg
     1741 tagcatctcg atcttgagcc ctttcccgta cgtgctttcg acgacctttt caggcctcag
     1801 cacgggtccg gccccgcggg agtcgcggag gtcaagggcc
//
LOCUS       DAOPOA010000531         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_68516_length_1839_cov_2.205717, whole genome shotgun sequence.
ACCESSION   DAOPOA010000531 DAOPOA010000000
VERSION     DAOPOA010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68516_length_1839_cov_2.205717"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1839
                     /locus_tag="PKJ17_10100"
     CDS             <1..>1839
                     /locus_tag="PKJ17_10100"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOD76370.1"
                     /translation="LKGDKEIGDKINKIIAKLAEANDLKGVIDQADFNDDGKLGRGKE
                     MVDRLSNLVAVFDKPELDFRKNRAENDDLLGDAYEYLMKNFATESGKSKGQFYTPAEV
                     SRVMAQVIGVGGATSGSQTIYDPTCGSGSLLIKAHDEALARTGYDLAIYGQEMDNATH
                     ALARMNMILHGCPTADIKKGNTLSTPSFTQGDTSLKTFDFVVANPPFSNKSWSNGLNP
                     AEDLFSRFALGIPPNKNGDYAFLLHLLASMKSTGKGAIVMPHGVLFRGNAEAEIRRSI
                     VKRGYIKGIIGLPANLFYGTGIPACIVVLDKENAAVRKGIFMIDASRGFIKDGNKNRL
                     REQDIHRIVSVFNSQAEVPGYSHLATFDEIEQNDFNLNIPRYIDSTEPEDLQDIDAHL
                     RGGIPDRDVDDLARYWAVFPGLRAELFEPAGRPGYSHLKVPTGSIRSTIFDHPEFEAF
                     NRAVTDPFDGWRAAHVRKLKDLGPGGHPRELISELSESLLDVFHGTPLIDPYDVYQRL
                     MTYWAETMQDDVYLIVGDGWREAARLRPVVDARVGKDKEKPDVTVGRQKYKADLAPAA
                     LMTARYFPGVQAAIDELETRRVALSQQMDELKDEQGGEEGLLSSVV"
BASE COUNT          403 a          547 c          582 g          307 t
ORIGIN      
        1 gctcaagggc gacaaggaga tcggcgacaa gatcaacaag atcatcgcca agctagccga
       61 ggccaacgat ctcaagggag tcatcgacca ggccgacttc aacgacgacg ggaagctggg
      121 cagaggtaag gagatggtgg accgcctttc caacctcgtg gccgtgttcg ataagcccga
      181 gctcgacttc cgcaagaacc gggccgagaa cgacgatctt ttgggcgacg cgtatgagta
      241 cctcatgaag aacttcgcca cagagtcggg gaagagcaag ggtcagttct acacccccgc
      301 tgaagtctcg agggtgatgg cccaggtaat cggcgtgggc ggcgccacga gcggcagcca
      361 gacgatctac gacccgacat gcgggtcggg ttccctgctg atcaaggccc acgatgaggc
      421 gctcgccagg accggctacg acctcgccat ctacggtcag gagatggaca acgcgactca
      481 cgccctggcc aggatgaaca tgatcttgca cgggtgcccc acggcggaca taaagaaggg
      541 caataccctt tccaccccga gtttcacgca gggcgacacc agcctcaaaa ctttcgattt
      601 cgtggtcgcc aatcctccgt tctcgaacaa gtcgtggagt aacggcctca atccggctga
      661 ggatttgttt agccgctttg ccctcggcat ccctccgaac aagaacggcg attacgcgtt
      721 ccttctccac ctgctggcat ccatgaagag caccggaaaa ggggcgattg taatgcctca
      781 cggcgtcctg ttcaggggta acgccgaggc cgagatccga aggagcatcg taaagcgggg
      841 ttacatcaag gggatcatcg ggttgccggc taacctgttc tacggcacag gaatccctgc
      901 ctgcatcgta gtgcttgaca aggagaacgc cgccgttcgg aagggcatct tcatgatcga
      961 cgcgagccgg gggttcataa aggacggcaa caagaaccgc ctgagggagc aggatatcca
     1021 caggatcgtc agcgtcttca acagccaggc ggaggtcccc ggctattccc acctggctac
     1081 cttcgacgag atagagcaga acgacttcaa cctcaacatc ccccgctaca tcgacagcac
     1141 tgagcccgag gacttgcagg acatagacgc gcacctgcgc ggcggcattc ccgaccgcga
     1201 cgtggacgac ctggcgcgct actgggcagt gtttcccggc ctacgcgcgg agctgttcga
     1261 accggctggc aggccggggt atagccacct caaggtcccg accggcagca tcaggtctac
     1321 gatctttgac catcccgaat tcgaggcgtt caaccgggcg gtgacggatc cgtttgacgg
     1381 gtggcgcgcc gcgcacgtgc gaaagctgaa ggacctgggc ccgggcgggc acccgcgcga
     1441 gctgataagc gaactgtcgg agagccttct cgacgtgttc catgggacgc ctctgatcga
     1501 cccctacgac gtctaccagc gcttgatgac ctactgggcg gagacgatgc aggacgacgt
     1561 ttacttgatc gtcggcgacg ggtggcgtga ggccgcccgg ctgcggcccg tggtagacgc
     1621 cagggtaggt aaggacaagg agaagcccga tgtgacagtg ggcaggcaga agtacaaggc
     1681 ggacctggcg cccgcggcgc tgatgacggc gcgttatttc cctggcgtcc aggccgcgat
     1741 cgacgaactt gagactaggc gcgttgccct cagccagcag atggacgagc tcaaagacga
     1801 gcagggcggg gaggaagggc ttctgtcctc ggtcgtgga
//
LOCUS       DAOPOA010000532         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_68774_length_1834_cov_2.627319, whole genome shotgun sequence.
ACCESSION   DAOPOA010000532 DAOPOA010000000
VERSION     DAOPOA010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68774_length_1834_cov_2.627319"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKJ17_10105"
     CDS             <1..283
                     /locus_tag="PKJ17_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76371.1"
                     /translation="EGCAAILWRDGSKGPLAADALKPTAHDLLKLKVADEIIHEPFGG
                     AHRDWQKTFDSTRVVLDRHLKELIEIDPEALKQMRYDKFRHMGVFEEKR"
     gene            280..1638
                     /locus_tag="PKJ17_10110"
     CDS             280..1638
                     /locus_tag="PKJ17_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76372.1"
                     /translation="MKTITMDLTNALAEAVGANGIDAGDLTTTLANISDYYARLLAKP
                     FAFMTLPETKFQFDEMRTLADKAAAMDIRNLVILGIGGSSLGTQTIFEALLHPLHNME
                     ERFRDGKPRYFILDNIDPHKMAAIVETIIPDIDRTFLVVISKSGETPETISQFMLFNE
                     LMRKSAGYQQRIVLITDKEKGLLNRIAEKEGYPTLNLPDGVGGRFSVLTPVGLFPSAL
                     MGLDIKRLSAGAAGMAAHTVRYDGEENMAFVLAAILYLMDRAGKKIHVVMPYCERLGG
                     FADWFRQLEAESLGKKGLGPTPTKSMGVTDQHSQLQLYVEGPKDKCVILFYSATQEVP
                     IPASFDYLEDVQYLANKDMKSLFHAEFQGTRLSLTEAQTPNISLILDEVSDYNLGALF
                     YLFEMVTAIMGHLLAVNAFDQPGVEQGKIYTKAMMGKKGLEKEKEHTEALLLRRAKTI
                     VF"
BASE COUNT          430 a          589 c          494 g          321 t
ORIGIN      
        1 cgagggctgc gccgccattc tctggaggga cgggtccaag ggacctctcg cggcggacgc
       61 gttgaaaccg acggcccatg accttctcaa gctcaaggtc gccgacgaga tcatccacga
      121 acccttcggc ggggcccaca gggattggca gaagacattc gacagcaccc gtgttgtact
      181 cgaccggcat ctcaaggaac tcatcgagat agatcccgaa gcactcaagc aaatgcgcta
      241 cgacaagttc cgccacatgg gcgtattcga ggagaaacga tgaaaacgat aaccatggac
      301 ctgacgaacg ctctggccga ggcggtcggg gcgaacggca tcgacgcggg cgacctcacg
      361 acgactctcg cgaacataag cgattactac gcgaggctcc tcgcgaagcc tttcgccttc
      421 atgacactgc cggagacaaa gttccagttc gatgagatgc ggaccctcgc ggacaaggcg
      481 gcggcgatgg acatcaggaa cctcgtcatt ctcgggattg gcgggtcttc gctggggaca
      541 cagacgatat tcgaagccct tctccatccc ctgcacaaca tggaggagcg tttccgcgac
      601 ggcaaaccgc gctacttcat cctcgataac atcgaccccc acaagatggc ggccatcgtg
      661 gaaacgatca tccccgacat cgaccgcacc ttcctcgtcg tcatcagcaa gtcgggagag
      721 acgccggaga cgatctcgca gttcatgctc ttcaacgagc tgatgcgcaa gtccgcgggt
      781 taccagcaaa ggatagtcct catcaccgac aaggaaaagg gcctcctcaa caggatagcg
      841 gaaaaggaag gctatcccac cctcaacctg cctgacggcg tgggcggccg gttctccgtg
      901 ttgacccctg taggtctctt cccgtcagcc ctcatgggac tcgacatcaa aaggctgtcg
      961 gcaggcgccg cgggcatggc cgcccacacg gtccgctacg acggcgaaga gaacatggcc
     1021 ttcgtcctcg ccgccatcct ttacctgatg gacagggcgg gaaagaagat acatgtcgtc
     1081 atgccctact gcgaacggct cggcggtttc gccgactggt tccggcagct cgaggcggag
     1141 agcctcggca agaagggact cggcccgacc ccgacgaaat cgatgggtgt caccgaccag
     1201 cactcccagc ttcagctcta tgtcgaaggc cccaaggaca agtgcgtcat cctcttctac
     1261 agtgccactc aggaagtccc catccccgca agcttcgact acctggaaga cgtccagtac
     1321 cttgccaaca aggatatgaa gtccctcttc cacgccgaat tccagggcac ccgcctgtct
     1381 ctcaccgagg cccagacccc caacatctcc ctcattctcg acgaggtcag cgactacaac
     1441 ctgggagccc tcttctacct cttcgagatg gtcaccgcca tcatgggcca cctcctagcc
     1501 gtcaacgcct tcgaccagcc cggagtggaa cagggcaaga tctacaccaa ggcaatgatg
     1561 ggaaagaagg gtctggaaaa ggaaaaggag cacacggagg ccttgctcct gcggcgggca
     1621 aagacgatag tcttctgacc ttcccgaagc ccattatttt gtcagatcct gtttaccctc
     1681 gtcattccgg cgaagaccgg aatccaggag agatcgccaa catgagatca cccggcaagg
     1741 aacggctggc aacaacacct ctctttagca tccgggatag aacgacgaat aggcgtacat
     1801 atcctcctcg tcatcctgag cattctccag cgcg
//
LOCUS       DAOPOA010000533         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_68817_length_1833_cov_4.014061, whole genome shotgun sequence.
ACCESSION   DAOPOA010000533 DAOPOA010000000
VERSION     DAOPOA010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68817_length_1833_cov_4.014061"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKJ17_10115"
     CDS             <1..354
                     /locus_tag="PKJ17_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOD76373.1"
                     /translation="KISDKSREWMTAEVDIILVTGGTGISRRDVTPEAVKSLGCREIP
                     GIGEYMRWKTSFENERSILSRSLAVACSGTIVITLPGSERGAVQCFSAVAPVLRHAVE
                     VATGRSGDCGGSRRG"
     gene            410..1258
                     /gene="rapZ"
                     /locus_tag="PKJ17_10120"
     CDS             410..1258
                     /gene="rapZ"
                     /locus_tag="PKJ17_10120"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOD76374.1"
                     /translation="MIITGMSGSGKSTALRMFEDLGFYAIENIPPTLLPQLLQVLGRH
                     EEAVQNGVAAVVDIRGESLLDDLFAVIASLKGRGLNIRVIFLDASDSCLVKRFEETRR
                     RHPLSSEITILEAIGRERRELSQIHEIADVVVETSDLTLSQLRNEIFAKMEITEGPLK
                     VIFTSFGFKHGIPQDSDFVIDVRFLPNPFYESKLRPLSGRDKEVQDFILSHDGVSNFF
                     SALSELMEQVTALYRNTGKSIVHIAVGCTGGRHRSVAVAEWLGSKMPDNSSIRHRDIE
                     KDTVVT"
     gene            1387..>1833
                     /locus_tag="PKJ17_10125"
     CDS             1387..>1833
                     /locus_tag="PKJ17_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOD76375.1"
                     /translation="MTKAFLHKVSQGPEIVAIGGGTGLSTLLSGLKYYTRNITAIVTV
                     TDEGGSSGRLRGEWGVLPPGDIRNCMLALAEDDSSLNRLLNYRFDRGELKGHSMGNLI
                     LLAATEMTGDFRKAVEELNRLLAIRGKVIPVTNESVVLCAATADGDL"
BASE COUNT          435 a          461 c          539 g          398 t
ORIGIN      
        1 aagatcagcg acaagagccg ggaatggatg acggcagagg ttgacatcat actggtcacg
       61 gggggaacgg gcatttccag aagggatgta acccctgaag cggttaaatc cctaggctgt
      121 agagagatac ccggcatcgg agagtacatg aggtggaaga catcctttga aaacgagagg
      181 tccatccttt ccaggtccct tgccgtggct tgttccggta caattgtgat cacgctgccg
      241 gggagcgaga ggggagcggt gcaatgcttc agtgccgtcg ccccggttct ccgccacgct
      301 gtggaagtag cgacggggag gagtggggat tgcggcggat ctcgccgagg ataattgaaa
      361 gaagggtccc gttttgaaag aatcccttcc taacagggtc tcaaggtgtg tgatcatcac
      421 gggcatgtca ggaagcggta aatccaccgc actgcgcatg ttcgaagacc ttgggtttta
      481 tgccatcgag aacatccctc ccacattgtt accccaactc ttgcaggtcc tgggccgaca
      541 cgaggaagcg gttcagaatg gagtggcggc cgtcgtagac atcagggggg aaagcctcct
      601 tgatgatctt ttcgccgtta tagccagcct caagggtaga ggacttaata tcagggtcat
      661 ctttctcgat gcctccgact catgcctcgt caagcgtttt gaagagacca ggaggaggca
      721 ccccctctct tcggagataa ccatcctcga ggctattggc agggagagga gagaactgtc
      781 tcagatccac gagatagccg acgtcgtcgt cgagacatcg gatctgaccc tttctcaact
      841 cagaaatgag attttcgcga agatggaaat aaccgagggc ccactcaagg tcatctttac
      901 ctccttcgga ttcaagcatg gcattcccca ggatagcgat tttgtcatcg atgtcaggtt
      961 cctaccaaac ccgttttacg aatctaagct gagacccttg agtggaaggg acaaggaagt
     1021 tcaggatttc atcctctccc atgacggggt atccaacttt ttcagcgccc tttcagaact
     1081 gatggaacaa gtcacggccc tttacaggaa cacgggaaag agcattgtcc atatcgccgt
     1141 cggctgtacc ggaggcaggc accgttcagt ggccgtcgcc gaatggctgg ggtcaaagat
     1201 gcctgacaat tcgtcgatca gacataggga tattgaaaag gatacggtgg taacgtgaac
     1261 tggtggtcgg ctttcaccat cgggtttctt acgggtggga taattgcacc tcttggtgcg
     1321 aggctcttcc aggaagaggt ggaagagggc gccggggaat cgcaagacaa aaagaatggc
     1381 tccgttatga ccaaagcctt cctgcacaag gtctcacaag gtcccgagat tgtcgctatc
     1441 ggtggaggga cgggcctttc aactttgtta tccgggttga agtattacac ccgcaatata
     1501 actgctattg tgacagttac cgatgagggg ggaagttccg gccgcctgcg tggtgaatgg
     1561 ggagtccttc ccccggggga tatcaggaac tgcatgctgg cccttgccga ggacgacagc
     1621 tctctcaaca ggctgctcaa ctacaggttc gaccgcgggg aactcaaggg gcacagcatg
     1681 ggcaacctca ttcttctagc agccacggag atgaccggcg acttccggaa ggccgtggaa
     1741 gaacttaaca gactcctggc catccgcgga aaggtgatac ctgtcacgaa cgagtcggtg
     1801 gttctttgcg ccgccacggc tgatggcgac tta
//
LOCUS       DAOPOA010000534         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_69350_length_1824_cov_3.895421, whole genome shotgun sequence.
ACCESSION   DAOPOA010000534 DAOPOA010000000
VERSION     DAOPOA010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69350_length_1824_cov_3.895421"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="PKJ17_10130"
     CDS             complement(<1..1105)
                     /locus_tag="PKJ17_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD76376.1"
                     /translation="MRIPVAIVTMFTFLWFVPTGHCAPYTLDDLYAIALERSERVKIS
                     EEDLAIARTGKDKAVSALLPKVSGLGNYTGYKDQKISDTGSLTQPDEASAWSLRLDQS
                     VSMSGREITAYKAAKDNIRKTDLDLFAVKEQYILSVTNAYYDVLKASRLVEIQKQNVE
                     RLTKHRDAASIRLRIGEVTKTAVLRAEAELSGARSDLVKAQNLLKFTKAVLKRIVGLE
                     DEFDIVDTHNGGAAGLKDISEGNTDPVIGNCLGLELECLRQMAHVERAEVKSSGIQKD
                     IASSQVAIARGSYWPTLAVEGVFAQRKEIPETSGLVRDNLYGGVRIVFPFFEGGLRAA
                     EVREAESRKRQTDYAYDDLKKSVTVEVESSYLDF"
     gene            complement(1102..1707)
                     /locus_tag="PKJ17_10135"
     CDS             complement(1102..1707)
                     /locus_tag="PKJ17_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOD76377.1"
                     /translation="MNRRSSEETRGIINDAALRVFSRSGYDGASMRMIAGEAGISVGA
                     LYLYYRSKEELCSTVFKGLFNEFRDAIAERTREISDPAEQIRAYIGTYMEMARVHREF
                     IYTFNKDKGFAFGVEWKQKFFVELRRFLNGIIRRGIDEGIFADIEESEATKIVLSVLR
                     GYVVSIVIDPDNLFTADACTDILLNGLVKRRGPQKGADVPR"
BASE COUNT          371 a          565 c          471 g          417 t
ORIGIN      
        1 taaaatcaag gtaagagctc tcaacctcga cagtgacgct ctttttcagg tcatcgtacg
       61 catagtccgt ttgtctcttc ctcgattccg cctccctcac ctcggcggcc cttaagcccc
      121 cctcaaagaa cggaaacact attctgacgc ccccatagag attatcccgt acaagaccgc
      181 tggtctcggg gatctccttc ctctgcgcga acaccccttc aacggcaagg gtcggccagt
      241 aggagcccct ggcaatggca acctgggaag aggcgatgtc cttctggatg cctgacgact
      301 tcacctcggc ccgttccacg tgtgccatct gtcttaaaca ttcaagctcg agcccaaggc
      361 aattcccgat aacggggtcg gtattgccct cgcttatgtc cttgaggccc gcggccccgc
      421 cattatgcgt atcgacgata tcgaactcgt cctcgaggcc gacgatcctt ttcaggacgg
      481 ccttcgtgaa tttcaggagg ttctgcgcct tgacgagatc ggaacgcgcc cccgacagtt
      541 cagcctccgc tctcaacacg gccgtcttcg tgacctcccc tatcctcaac ctgatcgaag
      601 ccgcgtccct gtgcttcgtc aggcgctcca cgttctgctt ctgtatctcc acgagccggg
      661 acgccttgag cacatcatag taggcgttcg tcacggagag tatgtattgc tccttcacgg
      721 caaagagatc aaggtcggtc ttccttatgt tgtccttcgc cgccttgtag gccgtgatct
      781 ccctgcccga catggacacg gattggtcga gtcgcaggct ccaggcggag gcctcatcgg
      841 gctgcgtcag gctccccgtg tcggagatct tctggtcctt gtagcccgta tagttgccga
      901 ggcccgatac cttcggcaac agggcggaca cggccttgtc ctttcccgtc ctggcaatgg
      961 cgaggtcctc ttcggatatc ttcaccctct cggaccgctc cagggcgatc gcgtagaggt
     1021 cgtccagtgt gtagggcgca cagtgccccg tcggcacaaa ccacaggaag gtgaacatcg
     1081 tgactatcgc gacaggtatc ctcaccgggg aacgtccgct cctttttgag ggccccggcg
     1141 tttgacaagg ccgttgagaa gaatgtccgt gcacgcgtcg gcggtaaata ggttatcggg
     1201 gtcgatcacg atggagacga cataccccct gagaacgctg aggacgatct ttgtcgcttc
     1261 gctctcctcg atatcggcaa atatcccctc atcgatgccc cgtctgatga taccgttcaa
     1321 gaaccttcgg agctccacga aaaacttctg tttccattcc acaccgaagg cgaatccttt
     1381 gtccttgttg aaggtgtaaa tgaactcgcg atggaccctg gccatttcca tgtatgttcc
     1441 gatgtaggcg cgtatctgtt cggcgggatc ggagatctcc cgggtccttt ccgcgatggc
     1501 atcccggaac tcattgaaaa gacctttgaa aactgtggag cagagttcct ccttgctccg
     1561 gtaataaagg taaagcgccc ccacgctgat gccggcctcc cccgctatca tcctcatgct
     1621 ggctccatca tagcccgacc gcgagaagac ccggagcgcc gcatcattaa tgataccgcg
     1681 agtctcttca gatgaacgcc tgttcatgtg aacccgcgtt cagtctacag aacccgggaa
     1741 aaagagtcaa ggctaaaggc gactgcgaaa gtagttgggc atggacatac ttaccaccct
     1801 ggggtatacc cagaagaaaa ggtt
//
LOCUS       DAOPOA010000535         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_69419_length_1823_cov_2.419683, whole genome shotgun sequence.
ACCESSION   DAOPOA010000535 DAOPOA010000000
VERSION     DAOPOA010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69419_length_1823_cov_2.419683"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /gene="ilvN"
                     /locus_tag="PKJ17_10140"
     CDS             complement(<1..461)
                     /gene="ilvN"
                     /locus_tag="PKJ17_10140"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOD76378.1"
                     /translation="MRHTISLLVENEFGVLSRIAGLFSGRGFNIESLCVAESLDPTVS
                     TMTIVTTGNDAILEQILKQLNKLINVIKVVDFKEIDYVSREMVLVKVNAEEKTRPEIL
                     RMVEIFRGKIIDVSSRSYTIMITGDEDKLKAFISLIKPLGIKELVRTGPIA"
     gene            complement(470..>1823)
                     /gene="ilvB"
                     /locus_tag="PKJ17_10145"
     CDS             complement(470..>1823)
                     /gene="ilvB"
                     /locus_tag="PKJ17_10145"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOD76379.1"
                     /translation="EADIVGITRPCTKHSYLVRDVKDLARIIKEAFHIAASGRPGPVL
                     VDIPKDVSSAVGDFKYPEKAVIRSYQPTYVGHTGQIKKAMRLIMHSKRPVLYTGGGVI
                     LSNASQELTRFAEALSIPVTNTLMGLGGIPGNHPLFLGMLGMHGTYAANMAITESDLI
                     IAMGARFDDRATGKTDEFAPHARIIHIDIDPTTISKNIKVDIPIVGDTRNVLTQMLKI
                     VEEEKEVYRQYHRSIADWTDKIGKWKKDYPLTYQRNDTLKPQHVIERVFELTKGKAII
                     TTEVGQNQMWAAQFFKFLKPRTCLTSGGLGTMGYGFPAAIGAQVAFPKSLVVDIAGDG
                     SIQMNIQELATAVQYNLPVKVIILNNGFLGMVRQWQELFYEKRYTWTRLHSPDFVKVA
                     EAYGAVGLRIEKEADVDRVLKQAFKNGRPTFIDVHVSPEECVYPMVPAGASLKEMLLV
                     "
BASE COUNT          324 a          515 c          536 g          448 t
ORIGIN      
        1 atggcaatgg gtccggtccg tacaagttct tttataccca gcggcttgat gagactgatg
       61 aatgccttga gcttgtcttc gtcgccggtg atcatgatgg tgtatgaccg ggacgacacg
      121 tcaatgatct taccccggaa tatctccacc atcctcagga tctcagggcg tgtcttctcc
      181 tcggcgttga cctttacgag taccatctcc cgcgaaacat agtcgatctc cttgaagtcg
      241 acgaccttga tgacgttgat gagcttgttg agctgtttca ggatctgttc gaggatggcg
      301 tcgttccccg ttgtcacgat cgtcatcgtg gacacggtgg ggtcgaggct ctcggccacg
      361 cagaggcttt caatgttgaa gcccctgccg ctgaagagac ccgcgatcct ggacaggacc
      421 ccaaactcgt tctcgacaag aagcgatatg gtgtgtctca attttcctcc taaacaagaa
      481 gcatctcttt cagggacgcg ccggcgggga ccatgggata gacgcattcc tcgggactca
      541 cgtgaacatc gatgaacgtg ggccgcccgt tcttgaaggc ctgcttcagg accctgtcga
      601 catcggcctc cttctctatc ctgagaccca ccgcgccata cgcctcggca accttgacga
      661 aatccggcga atgaaggcgc gtccacgtgt atcgcttctc atagaagagc tcctgccact
      721 gcctgaccat gccgaggaag ccgttgttga ggatgatgac cttgacgggc aggttgtact
      781 gcacggccgt cgccagttcc tggatgttca tctggatgct gccgtctccg gcgatgtcca
      841 cgacaaggga tttcggaaac gccacctggg cgcctatggc tgcggggaac ccgtagccca
      901 tcgtgccgag gccgcccgat gtgagacacg tgcggggctt gaggaacttg aagaactggg
      961 ccgcccacat ctggttctgt cccacctcgg tggtgatgat ggccttgccc ttcgtcagtt
     1021 cgaagaccct ttctatgacg tgctggggct ttaacgtgtc gtttctctga taggtgaggg
     1081 ggtagtcctt cttccacttt cctatcttgt ccgtccagtc ggcgatgctt ctgtggtact
     1141 gccggtagac ctccttttcc tcttcgacga tcttcagcat ctgcgtgagg acattgcgcg
     1201 tgtcgcccac tatgggtatg tcgaccttta tgttcttgct tatcgttgtg ggatcgatat
     1261 cgatgtgtat gatcctggcg tgtggcgcga attcatccgt tttgcccgtg gccctgtcgt
     1321 cgaagcgcgc ccccatcgcg atgatgaggt ccgattcggt gatggccatg ttcgcggcat
     1381 acgtcccgtg catgccgagc atgccgagaa agagcgggtg gttccccggg atgccgccga
     1441 ggcccataag ggtgttcgtg accggtatgg acagcgcttc ggcgaaccgt gtcaattcct
     1501 gcgaggcatt ggacagaatg acgcctccgc cggtatagag aacgggtctt ttcgagtgca
     1561 tgatgaggcg catggccttc tttatctgtc ccgtgtggcc cacgtaggtg ggctgatagc
     1621 tgcggatgac cgccttctcg ggatacttga aatcgccgac cgccgaggac acgtccttgg
     1681 ggatatcgac gaggacgggg cccggtcttc ccgatgctgc gatgtgaaag gcctccttga
     1741 tgatgcgggc aaggtccttc acgtccctga cgaggtagct gtgcttcgtg cagggccggg
     1801 ttatgcccac gatgtccgct tcc
//
LOCUS       DAOPOA010000536         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_69606_length_1819_cov_3.814059, whole genome shotgun sequence.
ACCESSION   DAOPOA010000536 DAOPOA010000000
VERSION     DAOPOA010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69606_length_1819_cov_3.814059"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PKJ17_10150"
     CDS             complement(<1..420)
                     /locus_tag="PKJ17_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76380.1"
                     /translation="MWAADQMILPWLRAGAALVALLIPLFLAVPAPCGDRQHVVLEVI
                     LNTDNKGEFFVYLTADGDCLMSRDDLVAIGFDGPAGTILQTGGRTFISLRSMKDVAYH
                     FDDETVSLHITAAPGLLRKSSIDFRPARRPHVYYPRDT"
     gene            complement(399..1202)
                     /locus_tag="PKJ17_10155"
     CDS             complement(399..1202)
                     /locus_tag="PKJ17_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HOD76381.1"
                     /translation="MSRTDTLTWPVRKTGHRPVLPFILLAAIVLLSCAPASAQSFSVH
                     PTRVSFDRTTKAQKLIIRNKGATDLSLQVGAFKWSQNKDGRDVYEDTSDIVVFPPIFK
                     IRKGEQRLVRLGTTLGPTSREGAYRVFVEELPVADPKRSGPGINLVFKVGIPLFVSPL
                     QASREALGICLAIEEGAVVLSALNNDNAHAEIESVSIIGQDSREKEIFSKGFNGWYIL
                     SGSSRVYKVDIPPAICKRLASVHAVVRTARNSFEDVVEVTGGMCGPQTR"
     gene            complement(1210..1710)
                     /locus_tag="PKJ17_10160"
     CDS             complement(1210..1710)
                     /locus_tag="PKJ17_10160"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HOD76382.1"
                     /translation="MKRVISLSGLFCLAVLIVATGLSADAVAGTKTGNLEVTATCSAS
                     CEFLTQGTNLAFGPHDPLGGTETTAQASWSYRCVAGTKYKLYIDGERQMLLDGTDSGN
                     KLAFQLYKDDYSTVFPESESTAGELTAESNAETPFTINGKIAAGDGQSLKAGSYSKTL
                     TVTLLY"
BASE COUNT          353 a          525 c          530 g          411 t
ORIGIN      
        1 ggtgtcccgc gggtaataga cgtggggccg ccttgcgggc ctgaagtcga tggagctttt
       61 cctcaggaga cctggtgcgg ccgtgatatg gagcgacacc gtttcatcgt cgaaatgata
      121 cgccacatcc ttcatcgatc tcagggagat gaacgtcctt cctcctgtct gcaatatcgt
      181 gccggcgggg ccgtcaaagc cgatggcgac gagatcatcc ctgctcatga ggcagtcccc
      241 gtcagccgtg agatagacga agaactcgcc cttgttgtcc gtgttgagga tgacttcgag
      301 tacgacatgc tgccggtccc cgcacggtgc ggggaccgcg aggaacaagg ggatgaggag
      361 cgcaaccagt gccgctcccg cgcggagcca cggcaggatc atctggtctg cggcccacac
      421 atgccccctg tcacctccac cacatcctcg aaggaattcc gcgccgttct gacaaccgca
      481 tggacgcttg caagtctctt gcagatcgcc ggtggaatat cgaccttgta gaccctggat
      541 gagccgctga gtatgtacca gccattgaag cccttcgaga atatctcttt ctcccgggaa
      601 tcctgaccga tgatgcttac cgattctatc tcagcgtggg cattgtcgtt gttgagagcg
      661 ctgagaacga ccgctccctc ttcgatcgcc agacagatgc cgagcgcctc ccgggaggcc
      721 tgaagggggc tgacgaatag aggtatcccc accttgaaca caagatttat ccctgggccc
      781 gatcttttcg ggtctgccac gggcagctcc tctacgaaga ccctgtaggc accttcccgc
      841 gacgtggggc caagggtcgt ccccaggcgc accagcctct gttcgccctt cctgatcttg
      901 aagatcgggg ggaagaccac gatgtcggag gtatcctcgt acacgtccct gccgtccttg
      961 ttctgggacc acttgaaagc tcccacctgg agggaaaggt cggttgcgcc cttgttccga
     1021 atgatcagct tttgcgcctt cgtggtcctg tcgaaggata cccttgtggg atgcacactg
     1081 aagctttgcg cagatgccgg ggcacaggag agaaggacaa tggcggccag tagaatgaaa
     1141 ggcagaacag gacgatggcc ggtcttccgg accggccacg tcagggtatc tgtcctcgac
     1201 atattgttcc tagtagagca gtgttactgt cagggtcttg ctgtaagagc ctgctttcag
     1261 ggactggccg tctcccgctg ctatcttgcc gttgatcgtg aagggggtct ccgcgttcga
     1321 ctcggccgtc agttctccgg cggtcgactc cgattcggga aagaccgttg agtagtcgtc
     1381 cttgtagagc tggaatgcaa gtttgttgcc cgagtccgtg ccatcgagca gcatctgccg
     1441 ctcgccatct atgtacagct tgtattttgt acctgccacg cacctgtaac tccacgacgc
     1501 ctgggcggtg gtttccgtgc cgccgagcgg gtcgtgcggt ccgaatgcga ggtttgtccc
     1561 ctgtgtcagg aactcacagc ttgcggaaca ggtggcggtc acctccaggt tcccggtttt
     1621 cgttccggcg acagcgtccg cactcaagcc cgttgcgaca atgagcaccg ccaggcaaaa
     1681 caaccctgaa agactgatga cgcgtttcat tgtgtcctcc ttgcttttga tttatgtgta
     1741 tttttcaaca caagcggggt tgtaaagata ggccgctgtg ttcaatcgga cctgaaaaat
     1801 gacgaatcgt aaggatatg
//
LOCUS       DAOPOA010000537         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_69665_length_1818_cov_3.703347, whole genome shotgun sequence.
ACCESSION   DAOPOA010000537 DAOPOA010000000
VERSION     DAOPOA010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69665_length_1818_cov_3.703347"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PKJ17_10165"
     CDS             <1..824
                     /locus_tag="PKJ17_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD76383.1"
                     /translation="ARHADAIHAAVGIVLLCLGIIVFLSIIIVKKNRAERELRRYQDN
                     LEVLVEERTEGLNFANAALTAEIQEREQVEAALRESEEKYRVLIEKANEAVVIVQDET
                     FVFANPRMHDLAGVPAGTLEGRSFVDLIWPDDRDMVMGRYRKRISGESVPDAYDFRLV
                     GPEGAFIWVFLSATTIPWKGRPATLNLLTDISDRKLAEEERERLITELQEALSQVKAL
                     SGLLPICASCKRIRNDEGNWEQMEAYIRDRSEAKFSHGICPECARKLYADQSRKK"
     gene            937..1572
                     /locus_tag="PKJ17_10170"
     CDS             937..1572
                     /locus_tag="PKJ17_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76384.1"
                     /translation="MNSHFTIYTVSWVLYCLLALVMFLRDRRSYAVARREYWRDLVKP
                     WKVVTFLVAAISFMVMAPYTGDPTWDAFDAGFMSLFTFASAPFAVGSLYRMVTGRLPV
                     GQLVVISAVWMFSASWCYDIYIFLRDGSYPVTWSSNITASSLLYFLAGLLWNLDWKKG
                     KGVILSFREETWPYASRERVFGRIVWFALPVMILVAVLMLYFVWTYLRPSG"
BASE COUNT          354 a          524 c          568 g          372 t
ORIGIN      
        1 atgccaggca cgccgatgcc attcacgccg ctgtcgggat cgtcctttta tgcctcggca
       61 tcatagtctt cctgtcgatc ataatagtca agaagaacag ggccgagagg gagctgcgcc
      121 gctaccagga caacctggag gtcctggtgg aggaaaggac cgaggggctc aattttgcca
      181 acgcggcact gacggcagag atacaggagc gcgaacaggt cgaggcggcg cttcgggaga
      241 gcgaggagaa ataccgtgtt ctcatcgaga aggcgaacga agcggtcgtc atcgtgcagg
      301 acgagacctt cgtttttgcg aaccccagga tgcacgatct cgcgggcgta ccggccggga
      361 ccctggaggg caggtccttt gtcgatctca tctggcccga cgacagggac atggtcatgg
      421 gcaggtacag gaagcggatc agcggcgaaa gcgttcccga cgcgtacgat ttcaggctcg
      481 tcggccccga aggcgcattc atctgggttt tcctgtccgc caccacgatc ccgtggaaag
      541 gcaggccggc aacactcaac ctgctgacgg acatatcgga ccgcaagctc gcggaggagg
      601 agcgggaaag gctcatcacc gagcttcagg aggccctttc ccaggtcaag gcgttgagcg
      661 ggttgctccc catctgcgcc tcgtgcaaga ggataaggaa cgacgagggc aactgggagc
      721 agatggaggc gtacataagg gaccggtcgg aagcgaagtt ctcccacggc atctgccccg
      781 agtgtgccag gaagctctac gcagaccagt ccaggaagaa atgagccttc cctcttcccc
      841 ccgatgatgg cagtgatccg caggtcggcc gccccgtgtg gcgggatcct cggaaaccgc
      901 ttgaaaaaat gaaccgaacg ggtgaagctg acagggatga actctcattt caccatatac
      961 acggtatcct gggtcctgta ttgccttctg gcgctcgtca tgttcctccg ggacaggcgt
     1021 tcttacgcgg tagcccggcg ggaatactgg cgggacctcg tcaaaccatg gaaggtcgtc
     1081 acctttctcg ttgcggccat atccttcatg gtcatggctc cctacacggg cgatccgaca
     1141 tgggacgcct tcgacgcagg tttcatgtcc ctgttcacct tcgcgagcgc ccccttcgcc
     1201 gtggggtcgc tgtacaggat ggttacggga aggctgcctg tcggacagct cgtcgtcata
     1261 tccgcggtct ggatgttctc ggcgagctgg tgctatgaca tatacatctt ccttcgcgac
     1321 gggagttacc ccgttacgtg gtcctcgaac atcacggcat cctccctgct ctatttcctt
     1381 gccgggctcc tgtggaacct ggactggaag aaagggaaag gagtgatact gtcgttccgg
     1441 gaagagacgt ggccatacgc gtcccgggaa agggtattcg gcaggatcgt ctggttcgcg
     1501 cttcccgtga tgatcctcgt cgcggtgctc atgctgtatt tcgtctggac ctacctgcgc
     1561 ccttccggtt gagcgtcgat cctcaggcct gcgagcgggg gtgcgtgctg cccgctggtc
     1621 cggcttcctg agagggccgg gaccgatcct tttcccaaat agtggaaaga tcatccgcct
     1681 tgtcacgcct ttcgtttgag ccagaagtcg aaggaccgtc ccgggatgaa gatgtcgatg
     1741 ccgacggacg gttcgtcgag gggattcgtg acgacgtgct gttcattccc ggggatgtag
     1801 accgcgtcgc cctcatcc
//
LOCUS       DAOPOA010000538         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_69793_length_1816_cov_3.072118, whole genome shotgun sequence.
ACCESSION   DAOPOA010000538 DAOPOA010000000
VERSION     DAOPOA010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69793_length_1816_cov_3.072118"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1135)
                     /locus_tag="PKJ17_10175"
     CDS             complement(257..1135)
                     /locus_tag="PKJ17_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOD76385.1"
                     /translation="MNYDGLITIDSEICRKDGLCVRICPKVFTQAGPGSVPEVSRPEF
                     CNDCGHCLLVCPAGAIRHRNLEGKPITEVEKDLLPSFRQVQEMVRARRSIRNFLDKPV
                     ERELIEKVIDAARFAPSAKNTQSTRFIAVDNGETLRRIASMTAAWLGRSARKLRNPLV
                     RAVYLMRGLTTKEEYSRWIGQFELTARNMEQGIDTILYRAPALVLFHADKRVRFAEAN
                     ANLALQNATFAASALGLGTFYTGYVVSACARDRAIPDMLGVPKGHRVYAGMTLGHVKI
                     GFSRWIDRNPARIDWI"
     regulatory      1341..1406
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1453..>1816
                     /locus_tag="PKJ17_10180"
     CDS             1453..>1816
                     /locus_tag="PKJ17_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOD76386.1"
                     /translation="MNDTSSIASPIRHPALPVLNTIEKICKEYGILSLSRQVDACKDL
                     LARDPFIDVAILGQFKAGKSSFLNSIIGKPVLPVGAIPVTTVITRLSYGETEGATVTF
                     LDNRKSRIGLDEVELYISE"
BASE COUNT          407 a          539 c          499 g          371 t
ORIGIN      
        1 aacgggcaca atcaacatcc tgtgacttta tcgcacattg tgaaagaaaa gtaaattagg
       61 taatacggta gacgacgggg ttaaaaaccg ttggaacagt tagaactgtt gggacgttga
      121 agaggtgccg agcacaggcg gagaggattg tactggaagt tctcccctcc cacagtcacc
      181 accaacttac aactattcca aggattagag gggttccaac ggttctaacg gtgctgactg
      241 ttctaacgcc agcaatctat atccagtcta ttcttgccgg attccggtct atccagcggg
      301 aaaaaccgat cttcacatga ccaagggtca taccggcgta gacgcggtgt cctttcggga
      361 ccccgagcat atcggggatc gccctgtcgc gggcacaggc ggagacgaca taccccgtgt
      421 agaaggtgcc aaggccgagc gctgaggcgg cgaaggtggc gttctggagg gcaaggttcg
      481 cgttcgcctc ggcgaagcgg acgcgcttgt cggcatggaa caggacaagc gcgggggccc
      541 ggtacaggat cgtatcgatg ccctgctcca tgttccgcgc cgtaagctcg aactggccga
      601 tccagcggga atattcctcc ttcgtcgtca ggccgcgcat gagatagacg gcgcggacga
      661 ggggattcct gagttttctt gccgaccttc ccagccacgc ggccgtcatg gacgctatac
      721 gccgaagcgt ctccccattg tcaacggcaa tgaaccgcgt gctctgcgtg ttcttggcgc
      781 tgggagcaaa gcgggccgcg tcgatgacct tttcgatcag ttccctctcg acgggcttgt
      841 cgaggaaatt ccgtatggac cttcgcgccc taaccatctc ctgcacctgc cggaacgacg
      901 gcagcagatc cttttccact tcagtgatgg gctttccctc aagattgcgg tgccgtatcg
      961 cgccggcggg acagacaagc aggcagtgcc cgcaatcatt gcagaactcc ggccgcgaaa
     1021 cctcaggcac actgcccggc ccggcctgtg tgaaaacctt cgggcagata cggacgcaaa
     1081 gcccgtcctt cctgcatatc tcgctgtcga tggtaataag accgtcgtaa ttcatatctc
     1141 ccccaagaac cttccggaac gcgtgccgcg gacatgatcg ggtaattaaa actccgggcg
     1201 accaggcccc accatcacac ccatgccccg tggtacacga atcatatccc cctcgccgca
     1261 ggaagtcaac gcgggctgtc ggtaaaggca ggactcggaa gatggttttg gattgtaaaa
     1321 tttcccggat atatgatact tttttgaccg gtgatggggt tcaccttcta accgccctga
     1381 ccggggctga taactcctga caaatagtgg aagcctttcg cggtcatcca cggatcgctt
     1441 tcggaggagt tgatgaacga cacttcaagc attgcaagcc ccatccggca ccccgccctt
     1501 ccggtcctca ataccattga gaagatttgc aaggaatacg gcatcctgtc cctctcgcgg
     1561 caggtcgacg cctgtaagga cctgctggca agagatccct tcatcgacgt cgccatcctg
     1621 ggacaattca aggcgggcaa aagctccttt ctcaacagca tcatcggcaa gcccgtcctt
     1681 cccgtcggcg ccattcccgt aaccaccgtc atcacgcgcc tgagctacgg ggagaccgaa
     1741 ggggccacag tgacattcct cgacaacagg aaatcgcgga tcggcctcga cgaggttgaa
     1801 ctctatatct cagagg
//
LOCUS       DAOPOA010000539         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_70148_length_1809_cov_3.880844, whole genome shotgun sequence.
ACCESSION   DAOPOA010000539 DAOPOA010000000
VERSION     DAOPOA010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70148_length_1809_cov_3.880844"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PKJ17_10185"
     CDS             complement(<1..311)
                     /locus_tag="PKJ17_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOD76387.1"
                     /translation="MTAQMRGFGAMGIKIVDGDLLDQSVDAIVNPWNRNLIPWWLLLP
                     RGVSGAIKKRAGLRPFVELRRSGVLPLGGASVTSAGRLPFKAIIHVAGIGHDWRSSER
                     S"
     gene            840..1283
                     /locus_tag="PKJ17_10190"
     CDS             840..1283
                     /locus_tag="PKJ17_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOD76388.1"
                     /translation="MLAPTRILVPTDFSEYSGRALQQALDIAQQNHAKIFLLHVVHEV
                     VHRGNLEFTLPEELVRRMKDGAVASAEQSLHTQLHTFLQTNGLEVVADVRYGIPCDEI
                     LKEEKEKEIDLVIIASPGSAGLARHHIGNVARGVLKHSTCPVLLT"
     gene            complement(1396..>1809)
                     /locus_tag="PKJ17_10195"
     CDS             complement(1396..>1809)
                     /locus_tag="PKJ17_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOD76389.1"
                     /translation="KIGFPDILFADHEGKLPSNLVKEINRHPSIGAKILKPLDFLGPA
                     VDYVSAHHERLDGRGYPRGLKDGQISLGAQIISVADIYDAVTSERPYQETRTSAEALA
                     ILRGIAGTRIRADVVEALERVLAGKEIPRETGQQG"
BASE COUNT          384 a          526 c          542 g          357 t
ORIGIN      
        1 atggaccttt cagacgaccg ccagtcgtgg ccgatgcctg ccacgtgtat gatggccttg
       61 aacggcagcc ggcccgcgga ggtcaccgag gcgcctccca gggggaggac gccgctgcgc
      121 ctgagctcga cgaagggccg taacccagcg cgtttcttga tcgcacccga taccccgcgg
      181 ggcaacagca gccaccacgg gatcaggttc ctgttccatg gattgactat ggcatctacc
      241 gactggtcca acaggtcgcc atctacgatc ttgatcccca tcgctccaaa accccgcatt
      301 tgagccgtca ccggcggaat tcctacatgt tagaatcagc ggccgccatt gtcaaggacg
      361 gaccggactg ggggcggggc ggacgagcga gttcgccgtc cttatccacg accactatca
      421 gggcagagaa ttgggatcga agctggtggc tcaacttccg cgcctccgag cgagccatcg
      481 acgggttttc ccggaaacca ccggtcgtag cagatatgat tacaagaaaa ttgttgctat
      541 ggctacagac atcggcaagt tttttcgcta atgttataaa agttgagaaa ttcggatggg
      601 tcacctggtc gcaaccatcg tcgcgccagg gacgaggtcc ggagacgtat ccttggtaag
      661 ctgacgagcc ttttggctgt tgtcttgata ttatacataa ttagatcgac agtagcgggg
      721 taaggagaag atgccccgtg aactcctgca ggaacgttcc cggaccctct cgtttgagtt
      781 tgctgaacaa cgctgcgatc ggcagagcgg aagagccaca cgcaagggag ggaatggaaa
      841 tgcttgcacc aaccaggatc cttgtgccga cggatttttc ggagtattca ggccgggcgt
      901 tacagcaggc gctggatata gcccagcaga accacgcgaa gatcttcctg cttcatgtag
      961 tccacgaggt agttcaccgc gggaatctcg aattcactct tcctgaagaa ctggtacgaa
     1021 ggatgaagga cggggccgtg gcctcggcag agcagtcatt acacacacag cttcacacat
     1081 ttctgcagac caacgggctg gaggtagtcg ctgatgtccg gtacggcatc ccatgcgacg
     1141 agatactgaa ggaggaaaaa gaaaaggaga tcgatctcgt gatcatcgca tctcccggca
     1201 gcgcgggcct cgccaggcac cacatcggca acgtggcccg cggtgtcctc aagcactcca
     1261 catgccccgt gctcctgaca taatgaaagg ggcaccgggg ttcccccgcc ccggaacctt
     1321 cccggccggg actgcaacac ccggttgctt cggacggcag ggtccgggat gggggaaccc
     1381 gccgtcctga cacggtcagc cctgttgacc cgtctcccgg ggaatctcct tgcccgccag
     1441 aaccctttcc agcgcctcga cgacgtctgc cctgatgcgt gtgcccgcga tgccgcgcag
     1501 gatagcgagg gcctcagcgg aggttctcgt ttcctggtag gggcgttccg atgtgacggc
     1561 gtcgtaaatg tcggcaacgg atatgatctg ggctcccagg gaaatctggc cgtccttgag
     1621 cccccgggga taaccgcgtc cgtcaagcct ctcgtggtgg gccgaaacat agtctacggc
     1681 aggtcccagg aaatcgaggg gtttcaagat cttcgcgccg atggatggat ggcggtttat
     1741 ctccttcaca aggtttgagg ggagcttccc ctcgtgatcg gcgaagagga tgtccgggaa
     1801 cccgatctt
//
LOCUS       DAOPOA010000540         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_70591_length_1801_cov_3.159794, whole genome shotgun sequence.
ACCESSION   DAOPOA010000540 DAOPOA010000000
VERSION     DAOPOA010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70591_length_1801_cov_3.159794"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..884)
                     /locus_tag="PKJ17_10200"
     CDS             complement(69..884)
                     /locus_tag="PKJ17_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76390.1"
                     /translation="MRGLLGITIGLAFLAFMSYGCSNRDREASQPQAAKEQAAEPGTA
                     GGEQDISTAGRSHWGDKCRTHFYYVAPGESASLTFEAVVDAGLAKTAREEEPTLRKEE
                     LEAVGYSVSCSNRSVDTFSVSFPVWVLSEPAFAAKENERILYAPLSGWKVDWNRNGEG
                     TIVKAPGADGKFAVGRLPLEKLLGNRSAIDIKGPNGSVYSLTPSQKTLTEEFHILSVT
                     FDVTAPRETVLRQNTRRAGVSWRDGGIGFDRIYDRDNERYVISGPAHSRDCFE"
     gene            1249..1500
                     /locus_tag="PKJ17_10205"
     CDS             1249..1500
                     /locus_tag="PKJ17_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76391.1"
                     /translation="MKMFFMGYDDYFDEPITEAFAGAGVRSYLKLYKGTRSSGKANAI
                     FIPVSDEELPALLEVVRGLKAQYPDVGIRAFTFPLEECV"
BASE COUNT          373 a          509 c          489 g          430 t
ORIGIN      
        1 gcggcggaag caaagaagtg tttgttaaaa tgcacgtttg accgtcaaag ggctgatggt
       61 cttcgggtct actcgaaaca atccctgctg tgtgccgggc cggagatcac gtatctttcg
      121 ttgtcacggt catatatcct gtcgaacccg atgcctccgt ctctccagga cacgcccgcc
      181 cttcgtgtgt tttgcctcag gacggtttct ctgggggccg tgacatcgaa ggtgacggag
      241 aggatatgga attcctcggt aagggtcttc tgagaaggcg tcagggagta tacggagccg
      301 ttgggaccct tgatgtcgat cgcgctcctg ttaccaagca gtttttcaag ggggagcctt
      361 cccaccgcga atttgccgtc cgcgccaggc gcctttacga tagttccttc cccgttcctg
      421 ttccagtcca ccttccagcc actcagaggc gcgtacaata tccgttcgtt ttcctttgcg
      481 gcgaaggcgg gttcggatag gacccacacc ggaaagctca cggagaaagt gtcgacggaa
      541 cgattggaac aggatacgga atatcccacg gcttccagtt cctctttccg aagagtgggc
      601 tcttcctcgc gggctgtctt cgccaggccc gcgtcaacca ctgcttcaaa tgtcagggaa
      661 gccgattctc ccggtgcaac gtagtaaaaa tgcgtacggc atttatcgcc ccaatgcgat
      721 ctgcccgcgg tgcttatgtc ctgttccccg ccggccgttc ccggctcggc cgcctgttcc
      781 tttgccgcct gcggttggga ggcttccctg tccctgttcg agcatccgta agacataaac
      841 gccaggaacg ccagacctat cgtaataccc aagagacctc tcatgttctt cctcaccgca
      901 tctcctttgc cctgtctgat ggatcgcacc ggctattccg ttgcagcgga cacgggccgt
      961 gatcaccttt cctccggctc ttgccgaaac gtacaacgtg aagcgtccgc gaaggcttgt
     1021 cttgaggcaa acagtcactc gtcgacataa catggttgtc gccggtaaca gcaaacaatt
     1081 aaacatatct gtgtacctgt agtcacccca aagggctggc actcaagcgg cccaagcgga
     1141 tcatgccccc ttttgttgct atttcatcat atctgtaata gtctcagaag gtagacggac
     1201 catgtcctca ggaacgcagg acacctttca cggcgagaag gagggacgat gaagatgttc
     1261 tttatgggtt atgacgatta cttcgatgag ccgatcacgg aggccttcgc gggggccggc
     1321 gtcaggtcct atttgaaact gtataaaggg accaggtcga gcggcaaggc caacgccatc
     1381 ttcatccctg tttccgatga ggagctcccc gctcttcttg aggtcgtgcg gggtctgaaa
     1441 gcacagtacc ctgatgtcgg tatcagggcg ttcacttttc ccctggagga atgtgtttag
     1501 gcacgagcca caggaccttc atcgaccgct ttcgcttgag gatacgtggc gggtattcat
     1561 ggcgcaaggc gtctcaatat cgggaggacc acaggatgcc cctcagcggc ccgtgacccc
     1621 gatcatagtg tgttgacaat gcgcactccg gtggctaaaa gtcgaaaaca agccttttcg
     1681 ttaagacctt ttgaagtgga tgggtcgctg tccggaatag aatgcagagg acaccttgcc
     1741 cggcggatcg acaccctctc tgtccattat gagataattg gagactcgct tggaattgat
     1801 c
//
LOCUS       DAOPOA010000541         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_70604_length_1801_cov_2.568156, whole genome shotgun sequence.
ACCESSION   DAOPOA010000541 DAOPOA010000000
VERSION     DAOPOA010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70604_length_1801_cov_2.568156"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..463
                     /locus_tag="PKJ17_10210"
     CDS             230..463
                     /locus_tag="PKJ17_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76392.1"
                     /translation="MGKNISVYLPDDQINFINAQKEGASQVVQKAIRLVMQAGKDRSG
                     YDDVTAAAADIGRSGGLEDVVRSWHADRDADRW"
     gene            457..849
                     /locus_tag="PKJ17_10215"
     CDS             457..849
                     /locus_tag="PKJ17_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOD76393.1"
                     /translation="MVGLDTGYFMGMMLGDRGIIGHWEALKEKEAAPCVSVLTLGEIL
                     YLTIRIGKPQQGRKMVEGIEKTCTVLDVNRAVVERAAALKGGHGTPYIDSIIIASFLE
                     NGCNEIHTKDRRHFGSVKDKSTKVVIWE"
     gene            1028..1579
                     /locus_tag="PKJ17_10220"
     CDS             1028..1579
                     /locus_tag="PKJ17_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOD76394.1"
                     /translation="MKATGQLRDEHGGIKVMLDVMERVCDTLKTTGGIDTEHFDGILE
                     FLKVFVDKCHHGKEEELLFPALVAAGIPEKGPIAVMLYEHEVGRAHVAAISQAYDEFT
                     AGDRSSSRQIMQEAHGYISLLRSHIDKENTILFVMADSHLSEQKQDELFEGFEKIEED
                     RIGLGKHEEFHGLIHNLSRIYLD"
BASE COUNT          477 a          430 c          553 g          341 t
ORIGIN      
        1 gtccccggct catcggaact gtgcccgttg tactacgaag aagagtgaga agataaagaa
       61 gctttcacgc tatcctcatg agaatggttg tttttcccga tcaacagcct gttgagtgcc
      121 agagggggag gtcaaagaca gggccgccga ttacgcctgg ccgtgttccg cacttttcgt
      181 tgtggactat tagatataat atgttataga gtaatacagg aggtgcggaa tggggaagaa
      241 tatatccgtg tatctgcctg acgatcagat caatttcatc aatgcccaga aggaaggcgc
      301 gagccaggtc gtacagaagg cgatacggct tgtcatgcag gcgggcaagg acaggtcggg
      361 ctacgacgac gtcacggcgg ccgccgccga catagggcgg agcgggggcc tcgaggatgt
      421 ggtaaggtcc tggcacgccg acagagatgc cgaccgatgg taggcctcga cacgggttac
      481 ttcatgggga tgatgctcgg agacaggggc atcatcgggc actgggaagc cctgaaggaa
      541 aaagaggccg cgccctgtgt gtccgttctg accctgggtg agatcctcta tctcaccata
      601 cgaataggca aaccacagca gggcaggaaa atggtcgaag gcatagaaaa gacgtgcact
      661 gtccttgacg tcaacagggc ggtggtagaa agggccgccg ctctcaaggg agggcacggc
      721 accccctata tcgattccat catcatagcg tccttcctcg agaacggatg caacgagatc
      781 cacacgaaag acagaagaca cttcggatcg gtcaaggaca agagcacaaa ggtcgttatc
      841 tgggaataga aagaacggca ccccctcacc ccgtcacccc ggcgaaggcc ggggtccagg
      901 aaaaaccttg ttgcagcaga aagcccacaa gaaatacacc cccaggagcg tgatatactt
      961 gatactatag tgcgacatgg caggcccgga gcggggtgtg tcatgtatcg ttcaggggag
     1021 ggatgatatg aaagctaccg gacagttgag agatgagcat ggggggatca aggtgatgct
     1081 tgatgtgatg gaaagggtct gtgacacatt gaagacaaca ggaggcatcg atacggaaca
     1141 ttttgatggc atcctggagt tcctgaaggt ctttgtcgac aaatgccatc atgggaaaga
     1201 agaggaactg ttgtttcccg cgttggtggc tgcgggcatc ccggagaaag gacctatcgc
     1261 ggtcatgctc tacgaacacg aagtgggaag ggcgcatgtc gcggcgataa gccaggcgta
     1321 tgatgaattc acagcgggtg acaggtcttc atcgagacag atcatgcagg aggcgcacgg
     1381 ttacatttct ctcctgagga gccacatcga taaggaaaac accatcctct tcgtgatggc
     1441 agacagtcat ctgtcggaac aaaagcagga tgaactgttc gaaggcttcg aaaagataga
     1501 agaagaccgc ataggcctcg gcaagcacga ggaattccac ggcctgatcc acaacctcag
     1561 caggatatac ctggattgaa cgcatcccgt cttcaccggg agttctcccc atgctcccca
     1621 cctccagaga ggagcgaaga gacgaacggg tcccgtaacg agtctactgg tagttccagg
     1681 tggtgccgat ctcggagagg ggaaggaggc ggacgttccc ggcgagggcg taggatgatt
     1741 tcccgggata gaaaggcctg gaatgacctc gatacggagt tcaatatcct tgaaatggtg
     1801 a
//
LOCUS       DAOPOA010000542         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_70762_length_1798_cov_2.751004, whole genome shotgun sequence.
ACCESSION   DAOPOA010000542 DAOPOA010000000
VERSION     DAOPOA010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70762_length_1798_cov_2.751004"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1386)
                     /locus_tag="PKJ17_10225"
     CDS             complement(76..1386)
                     /locus_tag="PKJ17_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOD76395.1"
                     /translation="MTATKSNSGTETPPASRAASLSRSLASVRLGIVVLSLIAATSIL
                     GSVIKQGGTEEEYLSLYSEKAYHFIKLFGFDDAYHSPWFYFLLALFALNLIACTVRRI
                     RGLTGERGPGAKNAPDVKALAAAGSGFSAPVDTLGEISRRLSASSYKRKQLSERTTLY
                     EKGSLARYGVLVIHASVLIVLLGGLIGLVAGDRGFLVLRVGETAEAAVSRERARLPVP
                     LGFTVRCKDFNVTFYPGGQPKEYASDIEILDGAGKVLKEGRIKVNEPLSYNGVRLYQS
                     SYGTSNSYTFRVDGREVVLAEQEVAKAGRTAFMVVRYAPQVHDFGPGVMVAYMDGEEP
                     KTLWFLRDVERMRTRVIEGSRISLEGITGGYYTGLEVSRDPGVPVVLAGFALMLVGLY
                     INFFTFHRRIYVVSDGAEIHVGGVAARNREGFLEELRRLSKDLA"
     gene            complement(1364..1747)
                     /locus_tag="PKJ17_10230"
     CDS             complement(1364..1747)
                     /locus_tag="PKJ17_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD76396.1"
                     /translation="MRKEVRIALGLAVVVVFVLTLLAGAFAQKKAPESMMLKLEGAKF
                     PPVAFSHPQHTEKAKIDCVECHHKDKNPKEPGGCMPCHDLKDVKNGAIPIKDAYHKNC
                     IECHKQSSAKGVKAPTKCNDCHKKQ"
BASE COUNT          340 a          568 c          517 g          373 t
ORIGIN      
        1 cgaaatagag gatgtacagg aagaagaggg cgaggtagag gatcgtcgag atcccgagga
       61 tcatgtcgtg cgttatcatg cgaggtcctt tgagagcctg cggagctctt cgagaaagcc
      121 ctccctgttc ctcgcggcca caccgccaac gtgtatctcg gcaccatcgc tcacgacgta
      181 tatcctccgg tggaaggtga agaagtttat gtagagcccg acgagcatga gggcgaaccc
      241 cgcgagcacc acaggcacgc cggggtcgcg cgatacctcg agacccgtgt aatatccgcc
      301 ggttatcccc tcgagggaga tccgcgagcc ctcgatcacg cgcgtcctca tcctttcgac
      361 atcccgcagg aaccagagcg tcttcggctc ttccccatcc atgtaggcga ccatcacccc
      421 ggggccgaaa tcgtgcacct gcggggcgta cctcacaacc atgaaagcgg tcctgcccgc
      481 cttcgccacc tcctgctccg cgaggacgac ctccctcccg tccacgcgga aggtgtagct
      541 gttcgatgtc ccgtagctcg actggtacaa cctgacccca ttgtaggaca ggggctcgtt
      601 caccttgatc ctgccctcct tcaggacctt gcccgcgccg tcgaggatct cgatgtcgct
      661 tgcgtactct ttcggctgcc ccccgggata gaaggtgacg ttgaaatcct tgcacctcac
      721 ggtgaagccg agcggtacgg gaagccgcgc cctctcgcgc gagacggcgg cctcggccgt
      781 ctcgcccacg cgcagcacaa ggaaacccct gtcgcccgca acgagcccga tgagcccgcc
      841 cagaagcacg atgaggacgc ttgcgtggat gacgaggacc ccgtatcgcg cgagagaccc
      901 tttctcgtaa agcgttgtcc tttccgacag ctgcttcctc ttatacgacg aggcgctgag
      961 gcggcgggag atctcgccga gagtgtccac gggggcgcta aagcccgacc ccgcggccgc
     1021 gagggccttc acgtccggcg cgttctttgc ccccggccct ctttcccccg tgagaccccg
     1081 tatgcgtctc acggtgcacg cgatgaggtt gagggcaaaa agggccagga gaaaatagaa
     1141 ccacggcgaa tggtaggcgt cgtcgaaccc gaagagcttt atgaaatgat aggccttctc
     1201 gctgtaaagt gagaggtact cctcctccgt gcccccctgc ttgatcacgc tcccgagaat
     1261 ggatgtcgcc gcgatgagcg acaacacgac gataccgagg cgcaccgatg cgagggatcg
     1321 ggagagggag gctgcccgtg aggccggcgg ggtttccgtt ccgctattgc tttttgtggc
     1381 agtcattaca cttcgtcggc gcctttaccc ctttcgccga tgactgtttg tgacattcta
     1441 tgcagttctt gtggtatgcg tccttgatcg gtatcgcgcc gttcttcacg tccttgaggt
     1501 cgtggcaggg catgcatccc ccgggctcct tcgggttctt gtccttgtgg tggcattcga
     1561 cacagtctat ctttgccttt tcggtatgct gaggatggga gaaagccacg ggagggaact
     1621 tcgccccctc gagcttcagc atcatcgatt ccggggcctt tttctgggca aaagcacccg
     1681 cgagcaacgt gaggacgaag acaacgacca ctgccagtcc gagtgcgatc cttacttcct
     1741 ttctcatgcc acacctcttc atcgggtttt caaatactca gaatgttatc gcaagttt
//
LOCUS       DAOPOA010000543         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_71053_length_1793_cov_3.128884, whole genome shotgun sequence.
ACCESSION   DAOPOA010000543 DAOPOA010000000
VERSION     DAOPOA010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71053_length_1793_cov_3.128884"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..530
                     /locus_tag="PKJ17_10235"
     CDS             48..530
                     /locus_tag="PKJ17_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOD76397.1"
                     /translation="MKIEERLVRTLTARSLSLCTAESCTGGLIAGRITAVPGASACFE
                     GGVVTYSNRAKAILLGVPVEVIEDHGAVSEEVARAMAEGARERLGTDIAVAVTGIAGP
                     AGGSPEKPVGTVFISLAVAGMTSVRGFRFEGTRREIRRRSGDEVLHFVLDHLEGKVNG
                     "
     gene            527..1075
                     /gene="thpR"
                     /locus_tag="PKJ17_10240"
     CDS             527..1075
                     /gene="thpR"
                     /locus_tag="PKJ17_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOD76398.1"
                     /translation="MRSFLAFEIPPEVKAYLSQVSRAMAKTLSGVRWVRPEGQHITIR
                     FFGEISEARAREIEDIVSAAGPYPGVGATLKRIDAFPDKRRPRVIVATIDEGVDIIRS
                     IYHDIENRLTALGYEREKRGFTPHITFGRVKVPAPLLERDTPPLECPRFAIERIILYK
                     SILGREGATYEPLFEKKLGGAA"
     gene            1135..>1793
                     /gene="recA"
                     /locus_tag="PKJ17_10245"
     CDS             1135..>1793
                     /gene="recA"
                     /locus_tag="PKJ17_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOD76399.1"
                     /translation="MNPKTLKGGIDVKDDDNRGKAIDMAVSQIEKLFGKGAIMKLGAK
                     ALEAVPVISTGSIALDLALGIGGIPRGRVVEIFGPEASGKTTLALQVVAEIQKTGGSA
                     AFIDAEHALDVSYAKRIGVNTDDLLISQPDTGEQALEISEILVRSGGVDVVVVDSVAA
                     LVPRAEIEGEMGDSHMGLQARLMSQALRKLTAITSKSMTTVVFINQIRHKIGIMFGNP
                     ET"
BASE COUNT          387 a          503 c          593 g          310 t
ORIGIN      
        1 gccggcatat tcgcgaacat ccttgttctc ctgtggacga ggttcatatg aagatagagg
       61 agagattggt caggaccctg acggcgagat ccctttcctt atgcaccgcc gaatcctgca
      121 cgggagggct catcgcgggc cggataaccg ccgtgcccgg agcgtcggcg tgcttcgaag
      181 gcggggtggt gacctacagc aacagggcga aggcgatcct tctcggcgtt ccggttgagg
      241 tcatagagga ccacggggcc gtgagcgaag aggtcgcccg ggcgatggct gaaggggcga
      301 gggaaaggct cgggaccgat atcgccgttg ccgtcacggg aatagccggg cccgccggcg
      361 gctcaccgga gaagcccgtc gggacggtct tcatctcgct ggcggtggcg gggatgacgt
      421 ccgtccgcgg gttccgtttc gagggcacga gacgcgagat acgccggcgc tctggagacg
      481 aggttctcca tttcgtgctc gaccacctcg aggggaaggt gaacggatga ggtcctttct
      541 tgcctttgag atccccccgg aggtcaaggc atatctctcg caggtgtcca gggccatggc
      601 gaagaccctt tcgggcgtaa ggtgggtcag gcccgagggg caacacatca ccatcaggtt
      661 cttcggggag atatcggagg cgcgggcgcg ggagattgag gacattgtca gtgcggccgg
      721 gccctacccg ggggttggag cgaccctgaa gcgcatcgac gcctttcccg acaagcgaag
      781 gccccgggtg atcgtcgcca ccatcgatga aggagttgac atcatacgct cgatatacca
      841 tgatatagag aaccgactta cagccctggg ttacgaacga gagaagcggg gtttcacgcc
      901 tcatatcact ttcgggagag taaaagtgcc cgcgcctctt ctggagagag acacccctcc
      961 cctggaatgt ccacggttcg cgatcgagcg tatcattcta tacaagagca tcctcggaag
     1021 ggaaggcgcg acatacgagc cgcttttcga aaagaaactg ggtggcgcgg cttgaactca
     1081 gccgcgggga agaagggaaa gagacccgat gcaggggaga gataaaggag aaagatgaac
     1141 ccgaaaacgc tcaagggagg catcgacgtg aaagacgacg ataacagagg caaagccata
     1201 gacatggccg tatctcagat agagaagctt ttcggcaaag gcgccatcat gaagctcggg
     1261 gcgaaggccc ttgaggccgt tcccgtcatc tccaccggct ccatcgccct tgatctcgct
     1321 cttggcatcg gcggcatccc ccggggcagg gtggtggaga tcttcgggcc cgaggcgtcg
     1381 ggcaagacga cactggcgct ccaggtcgtc gccgagatcc agaagacggg gggcagcgcc
     1441 gcgttcatcg atgccgagca tgccctggac gtcagctacg cgaaaaggat cggtgtcaac
     1501 accgacgacc tcctcatatc gcagcccgat acgggagagc aggccctcga gatcagcgag
     1561 atactcgtgc gcagcggagg ggtcgacgtg gtcgttgtcg attccgtcgc tgccctggta
     1621 ccccgggccg agatcgaagg ggaaatgggg gattcccaca tgggccttca ggctcggctc
     1681 atgtcgcagg ccctcaggaa actcacggcg atcacgagca agtccatgac caccgtcgtt
     1741 ttcataaacc agatccgtca caagatcggg atcatgttcg ggaatcccga gac
//
LOCUS       DAOPOA010000544         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_71115_length_1792_cov_2.586068, whole genome shotgun sequence.
ACCESSION   DAOPOA010000544 DAOPOA010000000
VERSION     DAOPOA010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71115_length_1792_cov_2.586068"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PKJ17_10250"
     CDS             <1..580
                     /locus_tag="PKJ17_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOD76400.1"
                     /translation="IVERTLGAGRGVIALTFHFGNWEIMGITSKLLNQPVVALARPLK
                     KHVRLNTFLNRLRESTGLTIIVNANTAREVMRLLKKNAIIAILGDQREKRSRGVFVDF
                     FGHKVPTSKGTAMIAMKTGCQVVPCYPVRKGFLRYTIMCGEPLVMERDGDIEDLIAKN
                     TRKINAFLEDIIRKYPDEWFWVHQRWGRKRRA"
     gene            complement(663..>1247)
                     /locus_tag="PKJ17_10255"
     CDS             complement(663..>1247)
                     /locus_tag="PKJ17_10255"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOD76401.1"
                     /translation="FLGEDRALRELEVTVYLEEKGFPVVSPVGYVAENQASSRKLYFL
                     SLFLPDAHDLMTHFASGGTGERLRMAKRLAEALFEMGRLGVYHPDLHLRNVLVTSSGG
                     LFLLDFDKAYRKEVTSADYERMFWRLDRFVRKYASLFGHPVDDRERLIFLRTYERLSG
                     DRIVERMAQKRDKMHRTSKLGWTIDRLIYRRKKT"
     assembly_gap    1248..1257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          366 a          569 c          485 g          362 t
ORIGIN      
        1 catcgtggaa agaacccttg gggcaggccg cggcgtcatc gcgctcacct ttcacttcgg
       61 caactgggag atcatgggca taacctccaa gctcctcaac cagcccgtcg tggccctcgc
      121 caggcccctg aagaaacacg tccgtctcaa cacctttctc aatcgcctcc gggaatcgac
      181 gggcctgacc atcatcgtca acgccaacac cgcgagggag gtcatgcgcc tgctcaagaa
      241 gaacgccatc atcgccatcc tcggagatca gcgcgaaaag cggtcccgcg gggtcttcgt
      301 cgatttcttc ggccacaagg tccccacatc gaagggcacc gccatgatcg ccatgaagac
      361 cggctgccag gtcgtcccct gctaccccgt acgcaagggc ttcctgcgct acaccatcat
      421 gtgcggcgaa cccctcgtca tggaacgtga cggcgacatc gaagacctca tcgcgaagaa
      481 cacccgcaag atcaacgcct tcctcgaaga catcatccgc aagtaccccg acgaatggtt
      541 ctgggtccac cagcggtggg ggaggaagag gcgggcttga atcgcttgag ccgcttgaat
      601 ggcttgaacg gggtaccgcc gacggacgtt gccgcgtgtc gccaggatct ggcgccggct
      661 tttcaggtct tctttcttct atagatcaaa cggtctatgg tccagcctag ttttgaggtg
      721 cggtgcattt tgtcgcgttt ttgggccatc ctctccacga tcctgtcgcc ggagaggcgt
      781 tcgtatgtgc gcaggaaaat gaggcgttcc ctgtcgtcta cggggtggcc gaagagagag
      841 gcgtatttcc tcacgaacct gtcgagacgc cagaacatcc tttcgtagtc ggccgatgtc
      901 acttccttgc ggtatgcctt gtcgaagtcg aggaggaaaa gccctccgct tgaggtcacg
      961 agaacgttcc tgagatgcag gtcgggatga tagactccca gccgacccat ttcaaagagt
     1021 gcttcggcga gcctcttcgc catccgcagg cgctccccgg tacccccgga ggcaaagtgc
     1081 gtcatgaggt catgggcatc gggcagaaaa agggagagga agtagagctt ccgggaggat
     1141 gcctggttct ccgccacata gccgacgggg ctgaccacgg gaaatccctt ctcttccagg
     1201 tagaccgtga cctcaagttc cctgagcgcc ctgtcctcgc caaggaannn nnnnnnngcc
     1261 cgttacccca cggagccatc cgccgtggag gtattgcctg cagacgaccg tcctgccccc
     1321 gagttcgaag acacccggcg cgccccgccc cccggggaac ctccttgcgg gccttacaat
     1381 cccggcaaca agctccctca tatccaccga ctcccctcgg tgaacaaagg tatacctccc
     1441 ttcccgttcc cttttgagtt ttgccatttt cctgattgta tacgatacaa acgcattttt
     1501 ccacggaaac aagagaaaag cccctggagg tctgcatacc tggcgaggca catgccagcc
     1561 atcaggaaca ttctgtttct ttgtctttaa cctgagacct gagacctgaa acccgcgacg
     1621 gtctttacac ttccatctcc atcccgtcct cccccagaag gtacggtgcg tggagaacgc
     1681 cggcccagtc gtcccagtcg gggtaaaggt gagtaaggat gacacgcttc ggctgcgctc
     1741 gttcaacgat cctgtcaagg gcggcaaggt tgaggtgccc cttcaccttt cg
//
LOCUS       DAOPOA010000545         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_71117_length_1792_cov_2.570524, whole genome shotgun sequence.
ACCESSION   DAOPOA010000545 DAOPOA010000000
VERSION     DAOPOA010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71117_length_1792_cov_2.570524"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>339
                     /locus_tag="PKJ17_10260"
     CDS             <1..>339
                     /locus_tag="PKJ17_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76402.1"
                     /translation="HVDGEYRNCSGCHDGKALSSKPGFILGADPSDKRVVTTLRQVPV
                     TRDSFLEAGVTMENFDVMPDYRAAPVHRIRRVTDRTRSCDVCHVNRKGFLTKESLIRR
                     GSRANEDVIF"
     assembly_gap    340..349
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<350..730)
                     /locus_tag="PKJ17_10265"
     CDS             complement(<350..730)
                     /locus_tag="PKJ17_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76403.1"
                     /translation="MDLNVAIVAPQTFFEVVDIIPVSPLDLAVTLPARNEDRFFLFKD
                     VPVETAGDPVARNASDISVYGLPMEGREFFAVMAINTVYSRARGRTRDRQGGEKKSEK
                     NSFCCPGRANSPHRYLLPHSIFNGA"
     gene            767..>1792
                     /locus_tag="PKJ17_10270"
     CDS             767..>1792
                     /locus_tag="PKJ17_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOD76404.1"
                     /translation="MIRSCTRCHSGDVYVKSGHGAAVMAGNLDSATCSDCHGLHDTKA
                     YHRSGGRYTLEARELYNKTCKSCHSDREIMLRNNISPQIVKYYEETYHGKVEDLGYPT
                     SVAGCADCHTDHNILPPSDPASILNPRNLIKNCSKCHAGIHPRFAEYKAHPDYRDVKK
                     YPLLYISFVAMAGLLLVVFTFYWTHTLLWWRKVYWEKHRLEEMGIKLESPFTHEEGVQ
                     EIKRFKTRDRIMHLFLVLSFFGLVITGFPLKYHDTLWAKLLIQAMGGAHNAGFYHRVA
                     ATVLIVLFLVTLARCLHFLFPKGLGKKARKGWVGRLFGPDSLMPNANDWRQFKQMVRW
                     FFNKGDYP"
BASE COUNT          421 a          499 c          486 g          376 t
ORIGIN      
        1 ccatgttgac ggcgaatacc gtaactgttc cgggtgccac gatgggaaag ccctgtcttc
       61 gaaaccgggc ttcatcctcg gggctgaccc ctccgacaag agggttgtga cgacactcag
      121 gcaggtcccg gtgacccggg acagcttcct cgaggcaggt gtcacgatgg agaattttga
      181 cgtaatgcca gattatcgtg ccgctcccgt gcacaggata cgaagggtaa ccgacaggac
      241 ccgctcatgc gatgtctgtc acgtaaacag gaagggattt cttacaaaag agtctctcat
      301 aaggcgcggc tcccgggcaa acgaagacgt gatcttcaan nnnnnnnnng gccccgttga
      361 atatggagtg aggcaaaagg taacgatgtg gcgaattagc tcgacccggg cagcaaaagg
      421 aatttttctc gcttttcttc tcgccgcctt ggcggtctcg ggtccggcct cgagcgcggc
      481 tgtaaactgt gttgattgcc atgacagcaa aaaattcacg accctccata gggagtccgt
      541 acacggaaat atcggatgca ttgcgtgcca cagggtctcc ggcggtctcg acaggcacgt
      601 ccttaaaaag gaaaaaccgg tcctcatttc gtgcgggcaa tgtcacagcg agatcgagag
      661 ggcttacagg aatgatgtcc actacctcaa agaaggtttg gggtgcgacg attgccacgt
      721 tcagatccat accttcaaac cgcgaaaaga caatatcaag aaggccgtga tacgaagctg
      781 tacgcggtgc cattccggcg atgtctatgt caaatcgggg cacggtgcgg cggtcatggc
      841 ggggaatctc gactccgcga catgcagtga ttgccacggc ctgcatgaca cgaaggccta
      901 ccaccgatcg ggagggcggt ataccctcga ggcgcgggag ctctacaaca aaacctgcaa
      961 atcctgccat tccgacaggg agatcatgct gcgcaacaac atttctccgc agatcgtcaa
     1021 atactacgag gagacatatc acggcaaggt ggaggacttg ggttatccga cttccgtcgc
     1081 gggatgcgcc gactgtcata cggaccataa catacttccc ccgtcggacc cggcttctat
     1141 ccttaacccc aggaacctca tcaagaactg cagcaagtgt cacgccggca tacatccccg
     1201 cttcgccgaa tacaaggccc atcccgatta ccgggacgta aagaaatatc cgctgctcta
     1261 cataagtttt gtcgccatgg cagggctgtt gcttgtggtc ttcaccttct actggaccca
     1321 tacactcctg tggtggagaa aggtctactg ggaaaaacac aggctcgagg aaatggggat
     1381 caagctcgag agccctttta cccacgaaga gggcgtccag gagataaagc gctttaaaac
     1441 gcgagaccgc atcatgcacc tttttctggt cctttccttc tttgggctcg tcattaccgg
     1501 tttcccctta aagtatcatg acaccctttg ggcgaaactg ctcattcagg ccatgggagg
     1561 ggcccacaac gcggggtttt accacagggt ggccgccacc gtccttatag tcctcttcct
     1621 tgtcacgctc gcgagatgtc ttcacttcct cttccccaag ggcctcggaa aaaaggcgcg
     1681 aaagggatgg gtcggcaggc ttttcggtcc cgactccctg atgccgaatg ccaacgactg
     1741 gcgccagttc aagcagatgg tgcggtggtt cttcaacaag ggcgattacc cg
//
LOCUS       DAOPOA010000546         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_71283_length_1789_cov_3.106690, whole genome shotgun sequence.
ACCESSION   DAOPOA010000546 DAOPOA010000000
VERSION     DAOPOA010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71283_length_1789_cov_3.106690"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PKJ17_10275"
     CDS             complement(<1..803)
                     /locus_tag="PKJ17_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76405.1"
                     /translation="MIEWSKTNDAIGTVNRGNPAESGLCTLCRADCKGRCETFLSSLK
                     GRKILYPRDFGIVTAGSANTTHVGVNYNALRIQGYLYGVRGLPKGLKNTADDCIFPNV
                     SIESEFGSKVITKTKMPIMTGALGSTFIAAKYWDSFATGAALVGIPIVVGENVVGIDK
                     EAVINNGKIEKAPELDRRIDTFLKYYDGYGAIIVQMNVEDTRNGVAEYVIDKYGDKVI
                     IELKWGQGAKDIGGEIQVDSLDYALFLKNRGYVVDPDPGVPEVQEAFKHG"
     gene            1040..>1789
                     /locus_tag="PKJ17_10280"
     CDS             1040..>1789
                     /locus_tag="PKJ17_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOD76406.1"
                     /translation="MDRKRKKDLLILGILRNSSTPLTSTKIAQELQSLGHDISERTVR
                     LYLQRMDTEGLARQARKKGHEITARGESELDSSRIIDRVGFLSAKIDRMTYQMSFDLN
                     TTSGSVVINVTIVDPRQLAKNVDYITRVYADGYAMGHLMAFLSPGETLGHISIPEGMI
                     GVGTVCSITLNGVLLKHGIPTVSRFGGLLEIEDKKPVRFVEIIMYDGTSIDPLEVFIR
                     SGMTNYMGAVDTGSGRIGASFREFPAESRDLV"
BASE COUNT          382 a          521 c          486 g          400 t
ORIGIN      
        1 ccgtgcttaa aggcctcctg gacctcagga acgccggggt cggggtcgac gacatagccc
       61 ctgttcttga ggaacagggc atagtcgagg ctgtccacct ggatctcacc gccgatgtcc
      121 ttcgcgccct ggccccattt cagttcgatg atgaccttgt cgccgtactt gtcgatgacg
      181 tattcggcca cgccgttgcg cgtgtcctcg acgttcatct ggacgatgat cgctccgtag
      241 ccgtcatagt atttcaggaa tgtgtcgatg cgcctgtcga gctccggcgc cttttcgatc
      301 ttgccgttgt tgatgaccgc ctctttgtcg ataccgacaa cgttctctcc gacaacgatc
      361 ggaatgccga cgagggcggc gccggtggcg aaggaatccc agtattttgc agcgatgaag
      421 gtggaaccga gggcgcctgt catgataggc attttcgtct ttgtgatcac cttcgacccg
      481 aattcggatt cgatgctgac gttcgggaag atgcaatcgt ccgccgtgtt cttgagtccc
      541 ttcggcaggc ctcgaacacc gtagagataa ccctggatcc tcagcgcgtt gtagttcacg
      601 ccgacatggg tcgtgttcgc gctgcccgcc gtaacgatac cgaaatctct cgggtagagg
      661 atcttcctgc ccttcaggct cgacaggaac gtttcgcatc tccctttgca gtccgcccgg
      721 cacagcgtgc acagccccga ttccgccggg ttaccacggt tcacggttcc aattgcgtca
      781 ttcgtcttgg accactctat catgtcaacc tccctaatgt attctctacg gtaacttcct
      841 gccactttac cggcaagcca ttgccgctta acttcggcaa agaatatcaa cacccggttt
      901 ccttgtcaac tagattttta taataattaa ggggttcgtt gcgattgttc ttgcaaaggg
      961 gtctttcatc ggatatcata acaacaccgg caaatttgcc gctaatagat ttggttttgc
     1021 cgcaaaggag cagcgtgaca tggacagaaa gagaaagaag gacctcctga tcctgggcat
     1081 cctcaggaat tcctccacac ccctcacaag tacgaagata gcccaggaac ttcagtctct
     1141 cgggcacgat atcagcgaaa ggaccgtcag gctctacctg cagaggatgg atacggaggg
     1201 cctggcccgc caggcacgca agaagggcca cgagataaca gcccggggag agagcgagct
     1261 cgactcctcc cgtataatcg accgcgtggg attcctctcg gcaaagattg accgcatgac
     1321 ctaccagatg tccttcgacc tcaatacgac atcgggcagc gttgtcatca acgtgaccat
     1381 cgtggaccct cgtcagctgg cgaagaatgt ggactacata acacgagtgt atgccgacgg
     1441 ctatgcgatg ggccacctca tggccttcct gagcccgggg gagaccctgg gacatatcag
     1501 catacccgaa ggaatgatcg gtgtcggaac ggtatgctcc ataaccctca acggcgttct
     1561 cctcaagcac gggatcccca ccgtctcgcg cttcggaggg cttctcgaga tcgaggacaa
     1621 gaagccggtc cgtttcgtgg agatcatcat gtatgacgga acgagcatcg atcccctgga
     1681 ggtgtttatc cgcagcggga tgacaaacta catgggagcg gtcgacacgg gaagcggcag
     1741 gatcggcgcc agtttccgcg agttccccgc cgagagcagg gacctcgtc
//
LOCUS       DAOPOA010000547         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_71452_length_1786_cov_3.867707, whole genome shotgun sequence.
ACCESSION   DAOPOA010000547 DAOPOA010000000
VERSION     DAOPOA010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71452_length_1786_cov_3.867707"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PKJ17_10285"
     CDS             <1..541
                     /locus_tag="PKJ17_10285"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOD76407.1"
                     /translation="EDDWESPTLGAWGLGWEVWLDGMEITQFTYFQQAGGITLSPVCV
                     EITYGTERIAMYLQDVDNVYDIRWNENVLYGDVFLEPEREFSIYNFEESDPVMLRTLF
                     DTFEGEGEKLVRNRGLIYPAYDFCLKCSHVFNLLDARGAISVTERANYIARVRNLAKM
                     CAELYVQKREELGFPLLKR"
     gene            590..>1786
                     /gene="glyS"
                     /locus_tag="PKJ17_10290"
     CDS             590..>1786
                     /gene="glyS"
                     /locus_tag="PKJ17_10290"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOD76408.1"
                     /translation="MSEFLLLEIGTEEIPARFLTPAKEGLSGLIREAFANTRIAFGDI
                     SIQATPRRMALFVQDIAEKQAETVTVKFGPPYNRAFDDAGNPTKAAVGFARSQGVEVG
                     DLTKGVKDGVEFVTVEKEEKGVPTVEMLPVLLPDIISRIPFQKKMRWGAVSFEYARPV
                     QWVLCLFGGTPVTFSVADVTSAPLSWCHRFLSPGPVGIGHPLEYVDALRKNHIIVNED
                     ERLEIIRQGIARIEETTGGKALRDEELVREILYITECPYPLKGSFDAVYLDIPKEVLV
                     NVMKSHQRYIPIEDASGRLLPHFIFFANTAPKDDANVIRGNEKVLRARLADARFFFDE
                     DVKAKLADRYERLASIVFHVKLGTLRDKMERVKAIAGYLASVVDHGVAGKLERLIPVM
                     KTDLVTH"
BASE COUNT          378 a          553 c          534 g          321 t
ORIGIN      
        1 cgaggacgac tgggaatccc cgacgctcgg cgcctggggc ctcggatggg aggtgtggct
       61 cgacggtatg gagatcaccc agttcaccta tttccagcag gccggcggta taaccctgtc
      121 gcctgtgtgc gtggagatca cctacggcac cgagcgcatc gccatgtacc tccaggacgt
      181 tgacaacgtt tacgacatca ggtggaacga gaacgtcctc tacggcgatg tcttcctcga
      241 acccgaaagg gaattctcca tttacaactt cgaggaatcg gaccccgtca tgctcagaac
      301 tcttttcgac accttcgaag gggaaggtga aaagcttgtc aggaaccggg ggctcatcta
      361 cccggcctac gatttttgcc tgaaatgctc gcatgtcttc aaccttctcg acgcccgcgg
      421 cgccatcagc gtgacggaac gcgccaacta tattgcgcgg gtgaggaacc tcgcgaagat
      481 gtgcgcggaa ctgtacgtgc agaagcgtga agaactcggt ttccccctgc tcaagcgata
      541 gcgggtcacc ccgcgcgggg cggcgggaac acggcaaggg aataacatca tgagcgaatt
      601 tctgcttttg gagataggca cagaggagat accggcgcgc ttcctcacgc ccgcgaaaga
      661 ggggctttcg ggcctcattc gggaggcctt cgccaacacg cggatcgcct tcggcgacat
      721 cagcatccag gcaaccccgc ggcgcatggc actcttcgtc caggatatcg cggagaagca
      781 ggcggagacg gtcaccgtca agtttgggcc cccgtataac cgcgccttcg acgacgcggg
      841 aaatcccacg aaggccgccg tcggcttcgc ccgttcccaa ggcgttgagg tcggcgatct
      901 cacgaagggc gtcaaggacg gcgtggaatt cgtgaccgtc gagaaagagg aaaagggggt
      961 ccccaccgtg gagatgctcc ccgtcctcct gcccgacatc atctcccgta tcccctttca
     1021 gaagaagatg cgctggggag ccgtgagttt cgaatatgcg cggcctgtcc agtgggtcct
     1081 gtgcctcttc gggggcacgc ccgtcacgtt ctccgtggcc gatgtcacga gtgcgcccct
     1141 gagctggtgc caccgcttcc tgtcgccggg cccggtcggg ataggtcatc ccctggaata
     1201 cgtcgacgcc ctcaggaaga accacatcat agtgaacgaa gatgaacgcc tggagatcat
     1261 acggcagggg atcgcccgca tcgaggagac aacgggcgga aaggcgctcc gggacgagga
     1321 gctcgtgagg gagatactct acatcaccga atgcccctat cccctgaagg gctccttcga
     1381 cgcggtgtac ctcgatattc ccaaggaggt cctcgtcaac gtcatgaaga gccaccagcg
     1441 gtacattccc atcgaagatg cctcgggacg gctcctgcct cacttcatat tcttcgccaa
     1501 cacggccccg aaagatgacg cgaacgtcat acggggaaac gagaaggtgc tccgcgcgcg
     1561 cctcgccgac gcgcgcttct tcttcgacga ggacgtgaag gcaaagctcg ccgacaggta
     1621 cgaacggctt gcctccatcg tcttccacgt caagctcggc accctcaggg acaagatgga
     1681 gagggtaaag gccatcgccg gatacctcgc gtccgtcgtc gaccacgggg tcgccgggaa
     1741 acttgaaagg ctcatccccg tgatgaaaac ggacctcgtt acccac
//
LOCUS       DAOPOA010000548         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_71747_length_1781_cov_3.365585, whole genome shotgun sequence.
ACCESSION   DAOPOA010000548 DAOPOA010000000
VERSION     DAOPOA010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71747_length_1781_cov_3.365585"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /gene="lysS"
                     /locus_tag="PKJ17_10295"
     CDS             <1..1196
                     /gene="lysS"
                     /locus_tag="PKJ17_10295"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOD76409.1"
                     /translation="KDVLKSPAYDIFKKFDICDIVGLEGKVFKTKTGELTVLAESMKL
                     LTKSLRPLPEKWHGLKDVEERYRKRYLDLIVNEKVRDVFTTRARIINYIRRYFIERDF
                     IEVETPMMQVIPGGATAKPFVTHHNAMGMDLFLRIAPELYLKRLVVGGFERVFEINRN
                     FRNEGISVRHNPEFTMLEYYQAYATYEDLMALTEDMVSSLVKEIFGTYTITYNEQQID
                     FTPPWRKITMAEAMAEYGNFDLSALNDPVRLAAYAKELEIEGAEKESKGKLITEIFEE
                     LCEKKLIQPTFITQYPIEVSPLAKRSAENPDITERFELYISGMEIANGFNELNDPEDQ
                     RERFEFQIAQKEEGATLDDDYITALEYGLPPTAGQGIGIDRLTMLLTDSASIREVILF
                     PLLRP"
     gene            1490..1780
                     /locus_tag="PKJ17_10300"
     CDS             1490..1780
                     /locus_tag="PKJ17_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76410.1"
                     /translation="MDIDRRGFIKYGVVGVGAAALLGSGVGASSARPVTGSDAGDGEF
                     VKPSGKEAHIAERSLSWWIERYKLPLHVYSADVIARNVRAFGKVFRDLYPKG"
BASE COUNT          402 a          502 c          530 g          347 t
ORIGIN      
        1 gcaaggacgt cctgaagagc cccgcttacg atatattcaa gaagttcgac atctgcgaca
       61 tcgtcggtct tgaaggcaag gtcttcaaga caaagacagg ggaactgacg gtgctcgccg
      121 agagcatgaa gctcctcacc aagtccctgc gccccttgcc cgagaaatgg catgggctca
      181 aggacgtcga ggagcgttac cgcaagcgct acctcgacct tatcgtcaac gagaaggtgc
      241 gggacgtctt cacaacgcgg gcacggatca tcaattacat caggcgctat ttcatcgagc
      301 gcgatttcat cgaggtggaa acgcccatga tgcaggtcat ccccggcggc gccacggcca
      361 agccctttgt cacccaccac aacgccatgg gcatggacct cttcctccgg atagcaccgg
      421 agctctacct caagcgcctt gtcgtcggag gtttcgagcg cgtcttcgag atcaacagga
      481 acttccgcaa cgaaggcatc tccgtgcgcc acaacccgga gttcaccatg ctcgaatact
      541 accaggccta cgccacttac gaggacctca tggccctcac cgaggacatg gtatcgtcgc
      601 tggtgaagga gatcttcgga acctacacca ttacatataa tgagcagcag atagacttca
      661 ccccgccctg gaggaagatc accatggccg aggccatggc cgagtacggc aatttcgacc
      721 tctccgccct taacgacccg gtccgccttg ccgcgtacgc gaaggagctc gagatcgagg
      781 gggccgagaa ggaatcgaag ggcaagctca tcaccgagat attcgaggag ctctgtgaaa
      841 agaagctcat ccagcccacc ttcataaccc agtaccccat agaggtctct cccctcgcga
      901 agcgcagcgc agagaacccc gatatcacgg agcgctttga gctctacata tcgggcatgg
      961 agatagccaa cggcttcaac gagctcaacg acccggagga ccagcgcgag cgcttcgagt
     1021 tccagatagc gcagaaggaa gagggcgcga ccctcgatga cgactatatc accgccctcg
     1081 aatacggtct tcccccgacc gccggccagg ggatcggcat agacaggctg acgatgctgc
     1141 tcacggacag tgcgtcgata cgggaagtga tactttttcc gctcttgcga ccgtaacagg
     1201 agtgaacggg cggacgggtc ggcgcttgtg cgcctaacgg gcggacgggt aaaagcagaa
     1261 aaggctttct gcgggttcga agggttgttc aatgtacccg ccttttccct gattgtttca
     1321 atccctgtca ttccggcgaa ggccggaatc caggaaatat gtgttgttgg aagaatctgt
     1381 ggattccgga tcaggccgaa acgacaggaa agatgggttt cttcatgagt ctgtcaaaga
     1441 caggaaagcc gttagcgctt tccataaccg ttcccgtgag gaggccgttg tggatatcga
     1501 taggcggggg tttatcaagt atggggttgt cggggtgggg gccgcggcgc ttcttggatc
     1561 gggtgtgggt gcgtcgagcg ctcgtccggt gacggggagt gatgcggggg atggagagtt
     1621 tgtgaagccg tcggggaaag aggcccatat cgccgagcgt tccctctcgt ggtggatcga
     1681 gcggtacaag cttcctttgc acgtctattc cgccgatgtc atagcccgga acgtgcgggc
     1741 cttcgggaag gtcttccggg acctctatcc gaaaggctag g
//
LOCUS       DAOPOA010000549         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_71917_length_1778_cov_3.409750, whole genome shotgun sequence.
ACCESSION   DAOPOA010000549 DAOPOA010000000
VERSION     DAOPOA010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71917_length_1778_cov_3.409750"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1552)
                     /gene="gdhA"
                     /locus_tag="PKJ17_10305"
     CDS             complement(194..1552)
                     /gene="gdhA"
                     /locus_tag="PKJ17_10305"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOD76411.1"
                     /translation="MSVTSEILEKIKKQDPHEFEFHQAAEEVLESLEPTAKKHPEFVK
                     AKIYERIVEPDRVVMFRVPWLDDKGELQVNRGFRIQFNNAIGPYKGGTRFHPSVNLGI
                     LKFLGFEQIFKNSLTTLAMGGAKGGSDFDPKGKSDNEVMRFCHAYMLELFRHIGPDID
                     VPAGDIGVGGREIGYMYGMYKKIRNDHTGVLTGKGLPFGGSLVRPEATGYGCVYFAAE
                     MLATKGLDFKGKTVAISGSGNVAQFAVEKVNQLGGRVVTLCDSSSTIVDGEGISGDKC
                     GYVMDLKNVQRGRISEYADKFGCAVCYIGESVWDVIKSQGLKVDIALPCATQNEIDGS
                     HAEALVKNGCVCVAEGSNMPSTPEAVRVFQDNGLLFGPGKAANAGGVATSGLEMSQNS
                     MRLSWTREEVDARLLGIMKSIHKSCLDAAEVYGKKGDYVFGANVAGFLKVANAMLAYG
                     YV"
BASE COUNT          331 a          565 c          491 g          391 t
ORIGIN      
        1 gtttcaggta atacctgatg gtccgctccg acaggtccgt cccctggcgg acaagacggg
       61 aggcaatatc ggaggagccc aagggggccc cttccctgtc aagcacggag agaatggaga
      121 acattatcct gttcatctac ccattccctc cgcgcagagt tttacacaaa aaagctccat
      181 tcaccggtcc ttatcacacg taaccgtaag cgagcatggc gttcgcgacc ttgaggaaac
      241 cggcgacgtt ggcaccgaag acatagtccc ctttcttgcc gtatacttcg gcggcatcga
      301 ggcatgactt gtggatgctc ttcatgatgc caagcagcct ggcgtcgacc tcttcccgtg
      361 tccaggaaag cctcatgctg ttctgggaca tctcgaggcc cgatgtcgcg actcctccgg
      421 cgttcgccgc cttgccgggg ccgaagagca ggccattgtc ctggaagacg cggaccgcct
      481 cgggagtcga aggcatattg gagccttcgg cgacgcagac gcagccgttc ttcacgagcg
      541 cttccgcgtg gctgccgtct atctcgttct gggtcgcgca cggcagagct atgtcgacct
      601 tgagaccctg gctcttgatg acgtcccaga cgctttcgcc tatgtagcag acggcgcagc
      661 cgaacttgtc ggcgtactcg cttatcctgc ccctctggac attcttgaga tccatgacgt
      721 aaccgcactt gtcgcccgat atcccctccc catcgacgat ggtggagctg gagtcacaga
      781 gggtgacgac ccttccgcca agttggttca ccttttccac cgcgaactgg gcgacgttcc
      841 cggaaccgct gatggcaacg gtcttgccct tgaagtcgag gcctttcgtg gccagcatct
      901 ccgcggcgaa gtagacgcag ccgtatcccg tcgcctcggg cctgacgagg ctgccgccga
      961 agggcaggcc cttaccggtg agaacccccg tatggtcgtt cctgatcttc ttgtacatgc
     1021 cgtacatgta acctatctcg cgccctccca cgccgatgtc tccggcgggc acgtcgatgt
     1081 cgggaccgat gtgcctgaag agctccagca tgtaggcgtg acagaaacgc atcacctcgt
     1141 tgtccgattt gcccttgggg tcaaaatcac taccgccctt ggcgccgccc atggcgagcg
     1201 tcgtgaggga attcttgaat atctgctcga acccgaggaa cttgaggatc cccaggttga
     1261 ccgagggatg gaagcgtgtg ccgcccttgt aaggtccgat agcattgttg aactggatgc
     1321 gaaaacccct gttgacctgc agttccccct tgtcgtccag ccaggggacc ctgaacatca
     1381 caacgcggtc gggctcgacg atcctttcgt atatcttggc ctttacgaac tcggggtgct
     1441 tcttcgccgt gggctcgaga ctctccagga cctcttccgc cgcctgatgg aactcgaact
     1501 catgggggtc ctgtttcttg atcttctcca gtatttcact tgtaactgac ataatggaat
     1561 cctccacctc tgtaatgtat tatcccggtc ccttcccggg ctctcacgca aaccatcccg
     1621 gaccgggccg gccggaaggc cgcaggcggc caccgcaccg ccggtccgtc gcccgtatcc
     1681 ggcggcgagc ctcgtggaaa tcaaaagcct gttcttcgag acattcctca gtagactgcc
     1741 aggggggtca cgctctgatc gtacacgttc agatcgct
//
LOCUS       DAOPOA010000550         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_72067_length_1775_cov_3.948837, whole genome shotgun sequence.
ACCESSION   DAOPOA010000550 DAOPOA010000000
VERSION     DAOPOA010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72067_length_1775_cov_3.948837"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKJ17_10310"
     CDS             <1..223
                     /locus_tag="PKJ17_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76412.1"
                     /translation="KYIPAMAPKITEARRMIDKAGRYIDLEVDGGIKAANAAEVVRAG
                     GNVLVMGTEIFHSGDYRKKIDEVHDKLGR"
     gene            226..870
                     /locus_tag="PKJ17_10315"
     CDS             226..870
                     /locus_tag="PKJ17_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HOD76413.1"
                     /translation="MELKRQKTYKGVFDRARDELLGSAMEEKFFLAGLGHDRVDSGTR
                     VHVPFFDETILFNIPGFTFSSSRGANVTLVAKIVILHYINTASGTPLTGDKVAYGDIP
                     ACMHYDPVFEKRVLKPLVRAFGYDRHTFLDAGRNLGAKEEGFGDASFTLFAFPKVPIT
                     FVLWEGDSEFPPSARALFDPSVTGYLPLEDIVVVSKLAAGRILKTAMKNFAEEV"
     gene            872..>1775
                     /locus_tag="PKJ17_10320"
     CDS             872..>1775
                     /locus_tag="PKJ17_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD76414.1"
                     /translation="MIFRQFNFDGCLAYVIACERERKGAIIDPSHEMDPFLDFAASYG
                     LAITHIIDTHTHVDHVSLAPELADRLGAKTVMSRNTPAQRELGAKVTDLFGIEKVLAE
                     NALKRVDLYLGDGDELKIGDITLQAMETKGHTRDHMAILTGDRIFTGDVLIIGQCGRT
                     DLPGGSPRDMHESLFGRIMPLSDDLIVYPAHDYRGNINSSLGYEKVNNVCLKTERTVE
                     EFEAFLKGLFPPLDATTGKLQCGLSTMEPAPEGELGTLMKSFCISMEAFLATPHEETV
                     IEPEELLQKIVQKERLFLVDVREPK"
BASE COUNT          423 a          529 c          505 g          318 t
ORIGIN      
        1 gaaatatatc cccgccatgg caccgaagat caccgaggcg cgcaggatga tcgataaggc
       61 gggcaggtac atcgacctcg aggtcgatgg cggtatcaag gcggcaaacg ccgccgaggt
      121 cgtcagggca ggcggcaatg tcctcgtcat gggcacagag atatttcaca gcggggacta
      181 ccggaagaag atcgacgagg tccacgacaa gctgggccgt tgatcatgga actcaagaga
      241 caaaagacat acaaaggcgt cttcgacagg gcacgcgacg agctcctggg aagcgccatg
      301 gaggagaagt tcttcctcgc gggcctcggc cacgaccggg tcgacagcgg gacgcgcgtc
      361 catgtcccct tttttgacga gaccatcctc ttcaacatac ctgggttcac cttttcgagc
      421 tccagggggg ccaatgtcac cctcgtcgca aagatcgtca tcctccacta cataaacacc
      481 gcttcgggga ccccgctcac gggcgacaag gtcgcctacg gggacatccc cgcatgcatg
      541 cactacgacc cggtcttcga gaaaagggta ctcaaacccc ttgtcagggc attcggctac
      601 gacaggcaca cctttctcga cgccggccgc aaccttgggg caaaggagga agggttcggc
      661 gacgcctcgt tcacgctttt cgccttcccg aaggtaccca taacctttgt tctctgggaa
      721 ggggacagcg agttcccgcc atcggccagg gcccttttcg atccctccgt gacgggttac
      781 ctgccgctcg aggatatcgt ggtggtatcg aagctagccg ccggaagaat actgaagacg
      841 gcgatgaaga actttgccga agaggtgtga catgatattc agacaattca atttcgacgg
      901 atgcctcgct tatgtcatcg catgcgaaag ggagagaaag ggggcgatca tcgacccctc
      961 ccatgaaatg gaccccttcc tcgatttcgc cgcatcttac gggctcgcca taacgcatat
     1021 catcgatacc cacacccacg tcgaccacgt ttccctcgcg ccggagcttg ccgacagact
     1081 tggagccaaa acggttatga gcagaaacac accggcacaa agggagttgg gggcaaaggt
     1141 cacggacctc ttcgggatcg agaaggttct tgccgagaac gccctgaaaa gggtcgacct
     1201 ctacctcggt gacggggatg aactgaagat aggcgatatc acactgcagg cgatggaaac
     1261 gaagggtcac acccgggacc acatggcgat cctcaccggc gacaggatct tcaccggtga
     1321 tgtccttatc ataggtcagt gcggaaggac ggacctgccc ggcggaagtc cgcgggacat
     1381 gcacgaaagc ctcttcggca ggatcatgcc cctctccgac gacctcatcg tctacccggc
     1441 ccacgactac aggggcaaca tcaattcctc actgggttac gagaaggtca acaacgtctg
     1501 cctcaagact gagcgcaccg tggaggaatt cgaggcgttc ctgaaaggtc tcttcccccc
     1561 tctcgacgcg acaacaggga aacttcagtg cggcctcagc accatggaac ccgccccgga
     1621 gggcgagctg ggaactctca tgaaaagctt ctgcatctcc atggaggcct tcctcgcgac
     1681 accccatgag gaaacggtca ttgagcccga agagttgctg cagaagatcg tgcagaagga
     1741 aaggctcttc ctcgtcgatg tcagggaacc gaaag
//
LOCUS       DAOPOA010000551         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_72149_length_1774_cov_2.724258, whole genome shotgun sequence.
ACCESSION   DAOPOA010000551 DAOPOA010000000
VERSION     DAOPOA010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72149_length_1774_cov_2.724258"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKJ17_10325"
     CDS             <1..556
                     /locus_tag="PKJ17_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76415.1"
                     /translation="VFMPTEQLLMIWTNREAEWGRIRELIPSPNVVFRIPVNGCPDNR
                     NVQANQWKVLALCNGMRSVSDIAETLGWQLFETSQTLCGMVKDGLLEKAAEKRVEQAD
                     RARRTVNGNFFPHIENELRKAMGPIAPIVIDDKLAEFGESREAFPEDRLSPFIQAIGE
                     EISDASKRVLFTRLMTEYIARKQK"
     gene            588..1538
                     /locus_tag="PKJ17_10330"
     CDS             588..1538
                     /locus_tag="PKJ17_10330"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOD76416.1"
                     /translation="MATENKVTSGLLFRLIAGIFVPILLAFAFLGCVLFLNIDLGGFK
                     FLSIRGIWSQSMNELGAASLKESTASLNRLGEQIVQQKAEDVAREIEIALGSAKKRPA
                     METLRNDGTLKQIAVQKVGVTGYTAVHDAQAVNHFHSNPKLVGSDLHDLSGKLPQFWK
                     LLEKGLAGPVGGYYNWRDADGSIRPKYMYIAPVKGAGLFVAATTYIDEFSKPAQAITA
                     KMGQMQKTYAEQYNRRFGLILIILAVVLLILLGVIYLYSFSVVHPIRHLSEVADKISM
                     GDLKATVDVKAKGEIAVLAESIERMQASVRAAIERLQKRR"
BASE COUNT          406 a          484 c          546 g          338 t
ORIGIN      
        1 ggtcttcatg ccgacggagc agctcctgat gatctggacg aaccgcgagg cggaatgggg
       61 gaggatcagg gagcttatcc cgtctccgaa cgtcgtcttc cgtatccccg tcaacgggtg
      121 cccggacaac aggaacgttc aggcgaacca gtggaaggtg ctggccctct gcaacggcat
      181 gaggtccgtt tcagacatcg ccgagaccct cgggtggcag ctcttcgaga catcgcaaac
      241 gctgtgcggc atggttaagg acggcctcct ggagaaggcg gcggagaaga gggtcgagca
      301 ggccgatagg gcgcgcagga ccgtgaacgg caatttcttt cctcacatcg agaatgagct
      361 gcggaaggcc atgggcccca tcgcccccat cgtcatcgac gataagctcg ccgaattcgg
      421 ggagtcgaga gaggcgtttc ccgaggacag gctctcgccc ttcattcagg ccataggcga
      481 ggagatatcg gacgcctcaa agagggtcct gttcacgcgc ctcatgaccg agtatatcgc
      541 acggaagcaa aaataactaa atcctacact tacaggaggg ggtttgcatg gctacagaga
      601 acaaggtgac atcgggatta cttttcaggc tcatcgcggg aatcttcgtc cccatcctgc
      661 tggctttcgc gtttctgggg tgcgtgttgt tccttaacat cgatctcgga ggcttcaaat
      721 tcctgagcat ccgcggtatc tggtcgcaga gcatgaacga actcggcgcg gcaagcctga
      781 aggaatcgac ggcatcgctc aacaggctcg gcgagcagat cgtccagcag aaggccgagg
      841 atgtcgccag ggagatcgag atcgccctgg ggtcggccaa aaaaaggcca gccatggaga
      901 ccctgcgcaa cgacgggacc ctgaagcaga tcgccgtcca gaaggtcggt gtgacgggtt
      961 atacggcggt ccatgacgcc caggccgtca accatttcca ctccaatccc aagctcgtgg
     1021 gatcggacct tcacgacctt tccgggaagc ttccgcagtt ctggaagctc cttgaaaagg
     1081 gactggccgg acctgtcgga gggtattaca actggaggga tgccgacggc agcatccgtc
     1141 cgaagtacat gtacatcgct cccgtgaagg gagcgggtct ctttgtcgcc gccacgacgt
     1201 acatcgacga gttctccaag ccagcccagg ccataacggc aaagatgggc cagatgcaga
     1261 agacgtacgc ggaacaatac aacaggcgtt tcggccttat cctcatcatc ctcgccgtcg
     1321 tgcttcttat cttgctcggc gtcatttacc tttattcttt ctccgtggtc catccgatcc
     1381 gtcatctttc ggaggttgcc gacaagataa gcatgggcga tctcaaggcg accgtggatg
     1441 tcaaggcgaa gggtgagatc gccgttctgg cagaatccat cgaaaggatg caggcaagcg
     1501 tgcgggcggc catcgagaga ctacagaaga gaagataaca gggaacgacc acaggttccg
     1561 ggggaagggg cctgtggtcc aacgaaagaa cagaggtcga tatgatatat ccacagggaa
     1621 aaacgatcca tcagaacctt tccgcggagt acacggatgt tccgcagctc ctgtcgaccc
     1681 ttacgggcaa tgggttttcg ggagtcgtgg agatagaggg gggaggcacg aaaggtgcct
     1741 tcttcatcgc cgggggccag atggtggacg cggt
//
LOCUS       DAOPOA010000552         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_72173_length_1774_cov_1.869692, whole genome shotgun sequence.
ACCESSION   DAOPOA010000552 DAOPOA010000000
VERSION     DAOPOA010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72173_length_1774_cov_1.869692"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="PKJ17_10335"
     CDS             complement(<1..1356)
                     /locus_tag="PKJ17_10335"
                     /inference="COORDINATES: protein motif:HMM:NF037897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76417.1"
                     /translation="MTTAISSANTSLRQVAATFKNKYFTPGDRNLDIGGGRFDYGTRF
                     LGEKGVENLVFDPYNRSPEHNRAIIERLRGGERFPTVTVNNVLNVIAEQDVRENVVLQ
                     AAKSLEDGGTAYFLIHEGDGGGQGRKTSKGWQNNRKAEQYMAEVETHFSAVERKGNLI
                     LAKGPVNIEGPAAWDASPDTADRTFFQDSIDTDYLAAVEAGDMETAQRMVDEAAKEAG
                     YGIGPVYHGTNAKFNTFDKDNVGTNYGKETPDHIGFFFTNRKEGDYPGGTAKDWARQS
                     VEKKGGTSVVVGAYLKINNPFTISDYAASIGDDIDTIMTYAGDRQPVVDIFDNDSQYI
                     LNKAMEMGKDGIVFVDAFEGDSDGLYVAFEPEQIKSADPVVKDNKGNIIPLSERFNSK
                     KSDIRYQKRGRKQQHRGSITLQREWGAQEETVLTLTPNADKSTFLHEIGHLFLWDLEQ
                     LE"
     gene            1328..1690
                     /locus_tag="PKJ17_10340"
     CDS             1328..1690
                     /locus_tag="PKJ17_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOD76418.1"
                     /translation="MFALLIAVVISFVGKLAAAIRSIPTPVMGGVCILLFGVIAAAGI
                     RMLVEKKVDYTKPRNLVLTSVVLVSGASGAAVKLGTVELKGMALGTFVAVALSLLFAA
                     FDRLGWTNERTAAGEPGA"
BASE COUNT          279 a          583 c          495 g          417 t
ORIGIN      
        1 ctccagctgc tccaggtccc acaggaaaag atgccctatt tcgtgcagga aggtgctctt
       61 atccgcgttc ggcgtcaggg tcagtactgt ctcctcctgc gccccccatt cccgctggag
      121 tgtgatcgaa ccccggtgct gctgcttccg cccacgcttc tggtaccgga tgtcggattt
      181 cttggagtta aaccgctcgg acaatggaat gatgttgccc ttattgtcct taactacggg
      241 gtcagcggat ttgatctgtt ccggctcaaa cgccacgtac agcccgtcac tgtcaccctc
      301 aaaagcgtcc acgaagacaa tgccgtcctt gcccatttcc atagctttgt tgaggatgta
      361 ctgagagtcg ttgtcgaaga tgtccactac cggctggcga tcaccggcat aggtcatgat
      421 cgtgtcaatg tcatctccaa tggaagcggc gtagtcggat atggtgaacg ggttgttgat
      481 cttgaggtat gcccccacaa ctacagacgt tccacccttc ttctcaacac tctgccttgc
      541 ccagtccttg gcggttcccc ccggatagtc tccttccttg cggttcgtga agaaaaatcc
      601 gatatggtcg ggtgtttctt tgccgtaatt cgtgccgaca ttatccttgt cgaatgtgtt
      661 gaatttggcg tttgtgccgt ggtatacggg cccgatgcca tacccggcct ctttcgccgc
      721 ctcgtccacc atccgctggg ctgtttccat gtcgcccgct tcgacggcgg cgagatagtc
      781 agtgtcgata ctgtcctgga aaaacgtccg gtcagcagtg tccgggcttg cgtcccaggc
      841 cgccggaccc tcgatgttca cgggcccctt cgccaggatc aggttgccct tccgctccac
      901 ggcggagaag tgggtttcta cctcggccat atactgctcg gccttccggt tgttctgcca
      961 ccccttggag gtcttcctgc cctgcccccc gccgtctccc tcgtggatca ggaaataggc
     1021 cgtgcctccg tcctccaggg attttgccgc ctgcaggacc acgttctccc ggacgtcctg
     1081 ctcagcgatg acgttgagga cgttgttgac ggtgaccgtc gggaacctct cgcccccccg
     1141 gagccgctcg attattgccc ggttgtgttc cggtgaccgg ttgtatgggt cgaacaccag
     1201 gttctctaca cccttctccc caaggaagcg agtgccgtag tcgaaacgcc cccccccgat
     1261 gtcaaggttc ctgtctccag gggtgaagta tttgttcttg aaagtggcag ctacctgccg
     1321 cagggatgtg tttgcgctgc tgatcgccgt cgtcatctcc ttcgtcggca agctcgccgc
     1381 cgcgatccgg agcatcccga cccccgtcat gggaggcgtc tgcatcctcc tcttcggcgt
     1441 catcgccgcg gcggggatcc ggatgctcgt cgagaagaag gtcgactaca cgaagccgcg
     1501 caacctcgtc ctcacctccg tcgtcctcgt cagcggcgcg agcggcgccg ccgtgaagct
     1561 cggcacggtc gagctgaagg ggatggccct cggcacgttc gtcgccgtcg ccctctccct
     1621 cctcttcgcc gcgttcgaca ggctcgggtg gacgaacgag cggacggcgg ccggcgaacc
     1681 cggcgcctga atcctctcgc cgactaggtc cgctcctcct ccgccctcga gtccggcagc
     1741 gttctgaccc ttaccgacga gaagatcgtg ggcg
//
LOCUS       DAOPOA010000553         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_72311_length_1771_cov_3.267483, whole genome shotgun sequence.
ACCESSION   DAOPOA010000553 DAOPOA010000000
VERSION     DAOPOA010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72311_length_1771_cov_3.267483"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PKJ17_10345"
     CDS             <1..635
                     /locus_tag="PKJ17_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOD76419.1"
                     /translation="VAGPPSAGKTAVVRQAIRSLLGGHRIAYLKIDVVRAFEDEELAT
                     EFGIPARKVYSGDLCPDHAGVMVMRDAIAWAGEEGADLLLVESAGLCLRCSPYTTQGL
                     GTAVLPVVAAEAPANLTIVCLDNGAFGSAGNQPTPASGQVDMELLALAAGIRNTCKVQ
                     NEEELVEAWESRGRGPNFIHVVLRPGNAQVPNIPLAPAEIRERFMQAIGG"
     gene            970..1254
                     /locus_tag="PKJ17_10350"
     CDS             970..1254
                     /locus_tag="PKJ17_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76420.1"
                     /translation="MEKSKNTLYILAAIGVTIISAVTGLWLIWGVVITLMFIDSLVSN
                     LKLWIDGKESRYLDVLQKTQFDSVESTELLRSMQASITRIEERLEALEKR"
BASE COUNT          363 a          488 c          534 g          386 t
ORIGIN      
        1 tcgtcgcggg gccgccgtct gccgggaaga ccgctgtcgt ccggcaggcc atccgctccc
       61 tcctgggagg gcaccggatc gcctacctca agatcgacgt cgtccgggcg ttcgaagacg
      121 aggaactcgc gaccgagttc gggatcccgg cgcggaaggt ctattccggc gacctctgcc
      181 ctgaccacgc cggggtgatg gtgatgcggg acgccatcgc ctgggccggg gaggagggtg
      241 ccgacctcct cctggtcgag agcgccgggc tctgcctgcg ctgctctccc tacacgacgc
      301 aggggctcgg aaccgccgtc ctcccggtag tggcagcgga ggcgccggcg aacctcacga
      361 tcgtctgcct ggacaacgga gccttcggga gcgcggggaa ccagcccacc ccggcctccg
      421 ggcaggtgga catggaactc ctcgccctcg ccgccgggat ccggaatacg tgcaaagttc
      481 agaacgagga ggaactcgtg gaggcctggg agagccgggg gcggggcccg aacttcatcc
      541 acgttgtcct caggcccggg aacgcacagg ttccgaacat cccgctcgct ccggccgaga
      601 tccgggagcg gtttatgcag gcgatcggtg ggtaggtatc cggctcgttt ctgtagtgga
      661 gcgaaagcac cctctcttct ttgtacatcc cgccgaatgt cgggtctatt ccagtggcga
      721 gtgcgcatca gctttggaca cccaattgtc ttcttagcgc attccggagc atgtttgctg
      781 gataaaaatc agtatccctt ctgtgagaat gggttgtctc gtctttctcc cccgcggggg
      841 atcaacctct ttattttcgg gaacatcccc tggacgtgta aaggaatgtg acagaattta
      901 tatgtagaac agcgttcatc tcattgtgtg gatttgagca ggcaaattta gaagaaaggg
      961 gttgtaatta tggaaaaaag taaaaatacc ttgtatattc ttgcagcgat aggagtgacc
     1021 attatcagtg ccgttactgg cctctggctg atctggggag ttgtcatcac actgatgttt
     1081 attgatagtc tcgtaagcaa ccttaaattg tggattgatg gtaaagagag ccgctacctt
     1141 gacgttttac aaaaaaccca gttcgattct gtggaaagca ctgagctgct acgttcgatg
     1201 caggcatcta taactaggat tgaagagcgg cttgaagcac tggaaaagcg gtaggaaata
     1261 acccacatca tatgcactat gatccggtat gttgttcccg agatgtaggg gcccatgtat
     1321 ataccgggcg atgcggtgca accggtgtcc ccggtctcgt cgatcaggag ccgaccgggg
     1381 caaccgtcgt gcagagaagg agaagaccga cgagagcggt agcacacgtt ccaaccgatt
     1441 tttctgtttc atgttcatct caccttcgtt ccaccttcgt tcacgagtat cctgggagat
     1501 cgtggagtcg cgacggttac ccctgatggt gggtctcgct ccggtagatc ggggcacgac
     1561 gccaatcgag acgcccggcc cccgatgacc gcatgaccgc tcctatgatc gtggtcctgg
     1621 ttaacgattc gtgaagggtt tctgccgcct atgagaccta tccgtcgtac atcccgggct
     1681 gcagggggaa agccggatag tcaccagcag aatgtctcca tatcatacgc ggcgtgcggc
     1741 atccccggtg gaaccaggtg actcatgtgg a
//
LOCUS       DAOPOA010000554         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_72326_length_1771_cov_2.522727, whole genome shotgun sequence.
ACCESSION   DAOPOA010000554 DAOPOA010000000
VERSION     DAOPOA010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72326_length_1771_cov_2.522727"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="PKJ17_10355"
     CDS             <1..1204
                     /locus_tag="PKJ17_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD76421.1"
                     /translation="SQLREFVEEVLKILYYYRGRVVFSFSGVALGILSICIIITTIDG
                     ANKKAQLIFEALGPNAIMIFGGGERQRAARIRVSTIRVGDADLLARVEGVYDVMKVFS
                     ARNVMMRYKDRNWQTQAIGSTTNYFESFSWGFQAGTVFTADDYDRAEAVCVIGAKVHE
                     ELFRGGPALGKTILVGKLPTRVIGVLEERGGSVGGPHVDDRVIMPLTTVTKRIVNERR
                     YLSMVRLKTHRDLDKTIEDVRATLRASHGLKGNLDDDFTIRSSKDILAFVTVISGSLL
                     LFLGTASIVALVVSGFVLANLFYLTIQERKKDIGIRRAYGATQKGILLSFLFESILIT
                     FIGGIAGIVLSIVLGGTFEKLFDIPMMFSFKVVIFALLFSFLTGLLSGLRPALRASRI
                     EPIEAIRG"
     gene            1201..>1771
                     /locus_tag="PKJ17_10360"
     CDS             1201..>1771
                     /locus_tag="PKJ17_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD76422.1"
                     /translation="MRYTASFYVRIAFQNLVVHKARMTLALLGILFAVMSLVAFGNIG
                     DGMKKKIESEIGKFGKNLVIVRAGLVHAAGRSTRQFSESKTLKLKDAELVKESIAGVV
                     EVVPYYDISYPARYRDSMLNVSVAGVSNRVFDIRNVDLAAGRYFTADEDAKAEKKAVV
                     GYKVVESLFSGEDPIGKNILVFRVPTEIVG"
BASE COUNT          368 a          465 c          555 g          383 t
ORIGIN      
        1 atcgcaactt agagagtttg tcgaagaggt cctgaagatc ctctactact accggggcag
       61 ggtcgtcttc tctttttcgg gtgttgccct cggtatcctc tccatctgca tcatcatcac
      121 caccatcgat ggggccaaca agaaggcgca gctcattttc gaggccctgg ggcccaatgc
      181 gatcatgatc ttcgggggcg gcgagaggca gcgggccgcg aggatacggg tgagcacgat
      241 cagggtaggc gacgccgatc ttcttgccag ggtggagggc gtctatgatg tcatgaaggt
      301 tttctcggcc cgcaatgtca tgatgcgata caaggacagg aactggcaga cccaggcgat
      361 agggtccacg acaaactatt tcgaatcctt ctcctggggc tttcaggccg gcaccgtctt
      421 tacggccgac gactatgacc gggccgaggc tgtctgcgtc atcggggcca aggtgcatga
      481 agagcttttc aggggcgggc ctgccctggg aaagacgatc ctcgtgggca agcttcccac
      541 cagggtcatc ggtgtgctgg aggagcgggg ggggagtgtc gggggacccc atgtggacga
      601 cagggtgatc atgcccctga caacggtgac aaagcgtatc gtaaacgaaa gacggtacct
      661 gagcatggtg cggctgaaga cgcacaggga cctcgacaaa accatagagg acgtgcgggc
      721 aacactgagg gccagccatg gtctgaaagg taacctcgat gacgatttca ccatcaggag
      781 ttcaaaggac atccttgcct tcgtgacggt gatctcggga tcgctccttc tctttctggg
      841 gacggcgtcg atcgtggccc tcgtggtgag cggtttcgtc cttgccaacc tcttttatct
      901 cacgatacag gaacgcaaga aggacattgg catcaggagg gcctatggcg cgacgcagaa
      961 gggcatcctc ctttctttcc tctttgagtc gatcctcatc accttcatcg ggggcatcgc
     1021 gggcatagtg ctcagcatcg ttctcggcgg caccttcgag aagctcttcg acattcccat
     1081 gatgttctcg ttcaaggtgg ttatttttgc ccttcttttc agttttctca cgggcctctt
     1141 gtcggggttg aggccggctt tgagggcgag caggatagaa ccgatagagg cgatccgggg
     1201 atgagatata ccgcttcctt ctacgtgagg atagcctttc agaacctggt cgtccacaag
     1261 gccagaatga ccctggcgct cctcggcatt cttttcgcgg tcatgagcct cgtggctttc
     1321 gggaatatcg gcgacggcat gaagaagaag atagaatctg agatcgggaa gttcggcaag
     1381 aaccttgtga tcgtccgggc cggcctcgtc cacgcggcgg gaagaagcac gcggcagttc
     1441 tcggaatcga agacgctgaa gctgaaagac gctgagctcg tgaaggaatc cattgccggt
     1501 gtcgtcgagg tggtccccta ttacgatatc agctatcctg cacgatacag ggacagcatg
     1561 ctcaacgtga gcgtggccgg ggtgtcgaac agggtgttcg acattcgcaa cgtggacctg
     1621 gcggcgggaa ggtacttcac cgccgacgag gacgcgaagg ccgagaagaa ggccgtcgtt
     1681 ggctacaagg tcgttgagag cctgttttcc ggcgaagacc ccatagggaa gaacatcctg
     1741 gtctttcgcg ttcccacgga gatcgtgggg g
//
LOCUS       DAOPOA010000555         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_72376_length_1770_cov_3.109621, whole genome shotgun sequence.
ACCESSION   DAOPOA010000555 DAOPOA010000000
VERSION     DAOPOA010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72376_length_1770_cov_3.109621"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PKJ17_10365"
     CDS             <1..1107
                     /locus_tag="PKJ17_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76423.1"
                     /translation="EAIGFGALAADCRFYSAYPMTPSTGIMLFLSSRAKDYGIIVEQA
                     EDEIAAINMALGASFAGVRSMTGTSGGGFALMTEGLSLAAMTETPIVIALAQRPGPAT
                     GLPTKTEQADLLFALNAGHGEFPRVIFAPGDPEQAVHLTAKAFDLSQKHQIPVFVMTD
                     QYLADTQWTMDGFSVDGLPMRDWRMDGKEIDGVDDYRRHAFTPSGVSPFAIPGKTRHV
                     VVTDSDEHSEAGHIIEDGPTRKKMVEKRLFLKMDNIRKDISPPLFLGQEEAETVLVSW
                     GSTYGVVREVVEAMPERKMAMLHFSEVFPLPGLEAFDYLDVLKRAKRTVSIEMNATGQ
                     FARLLRYETGYSVTDRVLRYDGRPFLVEELMEEL"
     gene            1107..>1770
                     /locus_tag="PKJ17_10370"
     CDS             1107..>1770
                     /locus_tag="PKJ17_10370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOD76424.1"
                     /translation="MEARDRYLGETPAWCPGCGNFSILNAFTAALEAEGIEPGGFCIV
                     SGIGQAGKFPHYVKCNTFNGLHGRALPVATGVKLANHELLVVAVGGDGDMYGEGGNHL
                     LHAMRRNIGVKVFVHDNQIYGLTKGQASPTSMEGMVTKNQPFGVLSEQLNPLAMAVAL
                     DCGFVARGYAGDREHLTELIRSAFHHDGLSLVDILQPCVSLNKVNTWEWYGKRVYRIG
                     EDH"
BASE COUNT          366 a          508 c          565 g          331 t
ORIGIN      
        1 gaggccatcg gtttcggcgc cctggcggcg gactgcaggt tctacagcgc ctatcccatg
       61 acgccatcca cgggcataat gctcttcctc agctccaggg caaaggatta cggcattatc
      121 gtggaacagg cggaagacga gatagcggcc atcaacatgg cgctcggagc ttcctttgcc
      181 ggggtcagat cgatgacggg aacatcgggg ggcggtttcg ccctcatgac cgaggggctt
      241 tccctcgcgg ccatgacgga gacccccatc gtcatcgccc ttgcgcagag accggggccg
      301 gcaacgggat tgccgacgaa gaccgaacag gccgatctcc tgttcgccct caacgccggc
      361 cacggcgaat ttccccgcgt catattcgcc ccgggcgacc cggagcaggc agtgcatctg
      421 acggcgaagg ccttcgacct ttcccagaag caccagatcc ccgtctttgt gatgaccgac
      481 cagtacctcg cggacacgca atggacgatg gacggcttct ccgtggacgg tcttccgatg
      541 cgcgattggc gtatggatgg caaggagatc gacggcgtcg atgactaccg aagacacgcg
      601 ttcacccctt cgggggtttc gcccttcgcc ataccgggaa agacgaggca cgtcgtcgtc
      661 accgatagcg acgaacacag cgaggcgggg cacatcatcg aggacgggcc gacgcggaag
      721 aagatggtgg agaagaggct cttcctcaag atggacaaca taaggaaaga tatctcacct
      781 cccctgttcc ttggccagga ggaggcggag actgttcttg tatcctgggg gtccacatac
      841 ggtgtcgtca gggaagtggt ggaagccatg ccggagagga agatggcgat gcttcatttc
      901 agcgaggtct tcccgttgcc cggtctcgag gccttcgatt atcttgacgt gctgaagcgt
      961 gccaaaagga cggtttccat agagatgaac gccacagggc agttcgcccg gttgttgaga
     1021 tacgagacgg ggtattccgt cacggacagg gtcctgaggt acgacggaag acccttcctc
     1081 gtggaggaac tgatggagga gctctgatgg aagcgcgcga caggtacctg ggggagactc
     1141 cggcatggtg tcccggttgc gggaactttt ccattctgaa cgccttcacg gcggcccttg
     1201 aggccgaagg catcgagcct ggcgggttct gcatcgtttc aggtatcggt caggcgggga
     1261 aatttcctca ttatgtgaaa tgcaacacct ttaacggact ccacggaagg gcgcttcccg
     1321 ttgcgacagg ggtcaagctt gccaaccacg agttgctggt cgtggccgtc ggcggggacg
     1381 gcgacatgta tggcgaggga ggcaatcacc ttctccatgc catgaggcgc aacatcggcg
     1441 taaaggtctt tgtccacgac aaccagatct acgggctcac gaagggccag gcctcgccga
     1501 cgagcatgga ggggatggtg accaagaacc aacccttcgg cgtcctgtca gagcaactca
     1561 accccctggc catggcggtg gcgctggact gcggttttgt ggcgagggga tacgcgggag
     1621 accgcgagca tctcacggag ctcatccgct cggccttcca ccacgacggg ttatctcttg
     1681 tcgacatact ccagccctgc gtttccctga acaaggtcaa cacctgggaa tggtacggga
     1741 aaagggtcta ccggatcggg gaggaccacg
//
LOCUS       DAOPOA010000556         1769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_72442_length_1769_cov_2.609102, whole genome shotgun sequence.
ACCESSION   DAOPOA010000556 DAOPOA010000000
VERSION     DAOPOA010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72442_length_1769_cov_2.609102"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..432
                     /locus_tag="PKJ17_10375"
     CDS             223..432
                     /locus_tag="PKJ17_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOD76425.1"
                     /translation="MKGTISIDRERCKGCELCVEFCPAKVIRLSDRLNSKGYFVAAFD
                     DGKECTGCGTCAVMCPEVAIEVMKS"
     gene            441..1499
                     /gene="vorB"
                     /locus_tag="PKJ17_10380"
     CDS             441..1499
                     /gene="vorB"
                     /locus_tag="PKJ17_10380"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOD76426.1"
                     /translation="MSGNAALGEAAILSGCSCYFGYPITPQNELTEYMAKRMGELGRV
                     FIQSESEIAAINMVLGASVAGARAMTSSSSPGMSLKQEGISYLAADELPAVVVDMVRG
                     GPGMGNISPAQSDYLQATRGGGHGDYKTIVLAPGSVQELTEVVPLAFDLADQYRNPVV
                     ILGDGMLGQMMEPVSFDNIKPPKEYPKDYTLTGAKGRPSRMVRSLLFDTKEEEEHNWK
                     LVRKYQRMEQNEVRYETFLTDDAEMIVIAYGIGARIVKGAIKRLRQENMKVGMVRPIT
                     VWPFPAAILQELAKTVHDFFVFEMSAGQMIEDVRLALAGKGRIHFYGRPGGIIPTPVE
                     LYRIIGRHYYMAARRKEQ"
     gene            1496..>1769
                     /locus_tag="PKJ17_10385"
     CDS             1496..>1769
                     /locus_tag="PKJ17_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76427.1"
                     /translation="MKKVYTRPKTMKQAHFHYCPGCGHGIINRLLMEVVDEMGLKESA
                     ICVAPAGCGMLVYNYFDIDTLESAHGRGAAVATGIKRVRPDNLVFSY"
BASE COUNT          409 a          478 c          530 g          352 t
ORIGIN      
        1 gtgtcccgga aaggcataat gttcctaccc tccccgcaac cacacatctt tgtcattttt
       61 cgtatgaatt aatatagaaa gaaccggcgg tgtttgcaac ttaattctgt ggatctccgg
      121 ggatgggcgg ggagggcaac ctcttccaca aaaaagttga caagcacatt cctgtgggct
      181 aatcttaaac gctttccaat ctgatgaggt ggttggttta ccatgaaagg tacgatcagt
      241 atcgaccggg aacggtgcaa aggctgcgag ctttgcgttg aattctgtcc cgcgaaggtc
      301 atcaggcttt ccgacaggct caacagcaaa ggctattttg tggccgcttt cgatgacggg
      361 aaggaatgca ccggttgcgg cacctgcgct gtcatgtgcc ctgaagtggc gatagaggtg
      421 atgaagagtt gaaaaagctc atgagtggta atgccgcgct tggagaggcg gcgatccttt
      481 cgggctgcag ctgttatttc ggatacccca tcacgcccca gaacgaattg acggaataca
      541 tggccaagag gatgggcgaa ttgggaaggg tcttcattca atccgaaagt gagatagcag
      601 ccataaatat ggtccttggc gcatccgtcg cgggcgcgcg ggcgatgacg tcctcgagca
      661 gccccggtat gagtctcaaa caggagggca tatcctatct cgcggccgac gagctgcccg
      721 ccgtcgtcgt cgacatggtg cgcggcggtc ccggcatggg caacatctcg cccgcccagt
      781 cggactacct tcaagccacg cgcgggggcg gtcacggcga ctacaaaacg atagtccttg
      841 cccccggttc ggtccaggaa ctgacggaag tggttccgct cgccttcgat ctcgccgacc
      901 aataccggaa ccccgttgtc atcctggggg acggtatgct ggggcagatg atggaacctg
      961 tcagtttcga caacatcaag cctccaaagg aatacccaaa ggactacacg ctgacgggcg
     1021 cgaagggacg gccctcacgc atggtcaggt cccttctctt cgacacgaag gaagaagagg
     1081 agcacaactg gaagctcgtc aggaaatacc agcggatgga acagaacgag gtccgctacg
     1141 agacgttcct gaccgatgat gccgagatga tcgtcatcgc gtacggcata ggggcccgga
     1201 tcgtcaaggg ggccataaag cgtctccgtc aggagaatat gaaggtgggc atggtgcggc
     1261 ccatcacggt atggcctttc cccgccgcca tcctccagga actcgcgaag acggtccacg
     1321 acttcttcgt tttcgagatg agcgcgggtc agatgatcga ggatgtcagg ctggcgctgg
     1381 caggaaaggg gcggatccat ttctacggca ggcccggcgg gatcatcccg acgccggtcg
     1441 agctctaccg catcatcggg cgtcattatt acatggcagc cagaaggaaa gagcaatgaa
     1501 aaaggtatac acgagaccga agaccatgaa gcaggcccac ttccattact gcccgggctg
     1561 tggccatggc ataatcaacc ggcttctcat ggaggtcgta gacgagatgg gcctcaagga
     1621 gagcgcgata tgcgtcgcgc ccgcggggtg cggaatgctc gtttacaact atttcgacat
     1681 agacacactg gaatcggccc atggtcgtgg agccgcggtc gccacgggta tcaagagggt
     1741 ccggcccgat aacctcgtct tttcctacc
//
LOCUS       DAOPOA010000557         1769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_72456_length_1769_cov_2.249708, whole genome shotgun sequence.
ACCESSION   DAOPOA010000557 DAOPOA010000000
VERSION     DAOPOA010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72456_length_1769_cov_2.249708"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1331)
                     /locus_tag="PKJ17_10390"
     CDS             complement(141..1331)
                     /locus_tag="PKJ17_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOD76428.1"
                     /translation="MASMKSDTARYHYIDNLRTMVIMLVIMLHLAVTYSGLGRWYYNE
                     DHAPELFGLVFFSLFQTFVQGFSMGMLFLVAGYFTPGALERKGLGRFVKDRWRRLGLP
                     SLFYLLVVTPFICWTELPSSQWRSSASNFPDFYSGYLMSGGMKLLGVGPMWFAVALLI
                     FSIMYALLRAVRPPAADTGHGQGTGSFGVLAALILIVTLGAFLLRLAFPLGTIFWGMQ
                     LCYFSQYVILFIAGILAYRTRFQERITPAVGRRWLIAGIVLGLPLLFAIKWMAGVYHF
                     PVHPAHVRSAYANVAGGFSWFSFSFALWESFVAVSMTVGLMALFRDKLGNGGSLARRL
                     SDSSFAVYMFHPPVIIAVSLAMQMLVTAPVVKWAIASLICVPLCFLLAYYILLRVPVL
                     NRIL"
     gene            1474..>1769
                     /locus_tag="PKJ17_10395"
     CDS             1474..>1769
                     /locus_tag="PKJ17_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOD76429.1"
                     /translation="MGAVGSLTGKDRSQCGLLIENGEGKVLLQLRDDNPDIPYPGCWG
                     TFGGQIEGDETPDEGIRREIWEELEYELSDPEYFGNFPFDGYDIYMYRTVDRGLT"
BASE COUNT          415 a          479 c          569 g          306 t
ORIGIN      
        1 tccgccattt cgttgcctcc ttttttcagg atcgtaagag caatatctgc cccgcacttt
       61 ttgttgtcaa ggatggcaga ccgggagagg ccggaaactc agggcagtac acgcctcccg
      121 gggtgggaga agggaaatgg ctaaaggatc ctgttcaaga cgggaacccg gagcagaatg
      181 tagtatgcca gcaggaagca gagggggacg cagatcaggc tcgcgatggc ccacttcacc
      241 acgggtgccg tgacgagcat ctgcatcgcc agtgatacgg cgatgatcac aggcgggtgg
      301 aacatgtaga ccgcgaagga gctgtcggac agtctccggg cgaggctgcc gccgttgccc
      361 agcttgtcgc ggaagagggc catgaggccc accgtcatgg aaacggcgac gaaggattcc
      421 cacagggcaa aggaaaaact gaaccaggag aatccccccg cgacgttcgc gtaggcgctc
      481 ctcacatggg cgggatgtac cgggaaatga tagaccccgg ccatccattt gatcgcgaag
      541 aggagtggaa gaccgaggac gatgccggcg atcagccacc tcctgccgac ggccggtgtt
      601 atccgttcct ggaaccttgt acgataggcg agtatcccgg cgatgaagag gataacgtac
      661 tgcgagaagt agcagagctg catcccccag aagatggtgc ccagagggaa ggcgagcctc
      721 agcaggaatg ctccgagggt gacgatcagg atgagcgcgg ccagtacgcc gaagctcccc
      781 gtgccctgcc cgtgcccggt gtctgcggcc ggcggccgga ccgcccgcaa gagggcgtac
      841 atgatcgaaa agatcagcag cgccaccgcg aaccacatgg gcccgacgcc gaggagcttc
      901 attccgccgg acattagata tccgctgtag aagtctggaa agttcgacgc gctgcttctc
      961 cattgcgacg acggcagttc cgtccagcat atgaaagggg tcacgacgag cagatagaaa
     1021 agcgagggca gacccagccg cctccagcgg tcctttacga aacggccgag acccttgcgc
     1081 tctagggcgc ccggcgtgaa atagccggcc accaggaaca gcatgcccat ggagaaaccc
     1141 tgcacgaatg tctggaagag actgaagaag acgagaccga agagttccgg cgcgtggtcc
     1201 tcgttgtaat accacctgcc gaggccgctg taggtcacgg cgaggtgcag catgataacc
     1261 agcatgatga ccatggtccg caggttgtct atgtagtgat atctggcagt atcgctcttc
     1321 atcgaggcca taagggaccg accctgtggg aattgtcgga agagtgcgtt atgagcataa
     1381 gctaccacac tatagtaggg tgggccggca tctgtcaaga caacatgccg tctctccgct
     1441 ttgttttgac gcggtgtcgt gataggtgtt accatggggg ctgtgggatc tcttacggga
     1501 aaggaccgaa gccagtgcgg cctgctcatc gagaacgggg aaggcaaggt cctgttacag
     1561 ctcagggacg ataatcctga cataccctat cccgggtgct gggggacatt cggcgggcag
     1621 atagaaggcg atgagacccc cgatgagggg atccggaggg agatatggga ggagctcgaa
     1681 tacgagctgt ccgacccgga gtacttcggg aactttccct tcgacggata cgatatctac
     1741 atgtacagga cggtcgacag gggcctcac
//
LOCUS       DAOPOA010000558         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_73128_length_1757_cov_3.772033, whole genome shotgun sequence.
ACCESSION   DAOPOA010000558 DAOPOA010000000
VERSION     DAOPOA010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73128_length_1757_cov_3.772033"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PKJ17_10400"
     CDS             <1..967
                     /locus_tag="PKJ17_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76430.1"
                     /translation="ALSFGIGGSGPSKKERDELERRIAKLEEELKKKGINPQMAYPTQ
                     MGYIPVGLGYMPGLISFEYPSPKMKRRIVVLPVTDNTNYKSEGLGELATKRLISRLES
                     TGTVICVDPNTLNVRGSYFDAVNAKKMNDDFGVQAILKTALSDVYTSSSRIEGKDEKE
                     TSFAMSKLAIDVFNTETGKIMKQLSGRNPISLSREKGDLSTEKAKIKAIDLAIEMIAE
                     DVLKTVLSIDWHSRVASVDGTKVYINAGRLSGLEKGRVLEVYAPGEEVIDPRTKQPLG
                     RKKGAYKGELEVVELFGVDASWAKTNKGGSFSSTDLVYVKQAAVK"
     gene            complement(1056..>1757)
                     /locus_tag="PKJ17_10405"
     CDS             complement(1056..>1757)
                     /locus_tag="PKJ17_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOD76431.1"
                     /translation="EHSMAEEKLRSSYEELRRLSAHIEEVRENERAAISRELHDVLGQ
                     ILAVINMDLRWLNKKIPAHEKELLGKVAAALTLVKQATRTIQKVSSGLRPVVLDDFGL
                     AAAVDHALKEFRDQTGITTSLIISGLVTDVGKDTSIALYRIFQEALTNVMRHSGATRV
                     EVSLQVEGDDIQLMVRDNGRGISKQQISDWNSLGILGMRERVSFIGGRIDITGSSGAG
                     TCITVDLPLKRTEAK"
BASE COUNT          394 a          485 c          509 g          369 t
ORIGIN      
        1 ggcgctttcc ttcggtatcg gcggcagcgg tcccagcaag aaggagcggg acgaactgga
       61 aagaaggata gcgaagcttg aagaggaatt gaagaagaaa gggattaatc cccaaatggc
      121 ctacccgacg cagatggggt atatccccgt cggtctgggg tacatgcccg gtctcatttc
      181 tttcgagtat ccgtcgccca agatgaaacg gcgcatcgtg gtcctccccg ttacggataa
      241 taccaactac aagtcggaag gcttgggaga gcttgccaca aagcggctca tatcccgcct
      301 cgagagcacg gggacggtta tatgcgtcga ccccaacacg ctcaatgtca ggggatccta
      361 cttcgatgcc gtcaacgcga agaagatgaa cgacgatttc ggcgttcagg cgatcctcaa
      421 gaccgccctt tccgatgtct atacgagttc atcgaggatc gagggcaagg atgagaaaga
      481 aacatccttt gctatgtcaa aactggccat cgacgttttc aacacggaga caggcaagat
      541 aatgaagcag ctttccggca ggaaccccat ctccctgtcc cgcgaaaagg gcgatctcag
      601 cacggaaaag gcaaagatca aggcgatcga ccttgccata gagatgatcg cggaagatgt
      661 cctgaagacc gtcctctcca tcgattggca ttcgcgtgtc gcctccgttg acgggacaaa
      721 ggtctatatc aacgcgggac ggctgtccgg cttggaaaag ggcagggtcc tcgaggtcta
      781 cgcacccggc gaggaggtca tcgaccctag gacgaagcag ccgctcggca ggaagaaagg
      841 cgcctacaag ggagagctgg aggtcgtgga gctctttggt gttgacgcaa gttgggccaa
      901 gaccaacaag ggcggcagtt tttcctccac cgacctcgtc tacgtgaaac aagcagcggt
      961 aaaataagat caaaaacagg taacggggtt acaaattccc gatgatgggt caccggattg
     1021 gtgcgtccgg cgaccgtatg cgaagattct gtctctcatt tcgcctccgt tcttttcagg
     1081 gggaggtcga cggtgatgca ggtcccggcc ccgctcgatc ccgtgatatc gatccttccg
     1141 ccgatgaaag agacgcgttc acgcattccc agtatgccaa gggaattcca gtcggatatc
     1201 tgctgcttcg agataccgcg gccgttgtcc ctgaccatca gttgaatgtc atctccctcg
     1261 acctgaagcg agacctccac gcgtgtcgct ccggaatggc gcatcacgtt cgtcagggcc
     1321 tcttgaaata tccggtagag tgctatgctc gtgtccttgc cgacatccgt aacgaggccg
     1381 ctgataatga gactggtggt tatacccgtc tggtcgcgga actccttcag ggcatggtcg
     1441 acggcggcgg cgagaccgaa gtcgtcgagg acgacgggcc tcaagcccga tgataccttt
     1501 tgaatggtcc ttgttgcctg tttgacgagg gtaagagcgg cggcaacctt gccgagcagt
     1561 tccttttcgt gcgccggtat cttcttgttg agccagcgca ggtccatgtt tatgaccgcg
     1621 aggatctgcc ccagaacgtc gtgcaactcc cgggagatgg cagcccgttc gttctcccgc
     1681 acctcctcga tgtgcgcgga gagccttcgc agctcttcgt aggatgacct cagcttctct
     1741 tcggccatgc tgtgctc
//
LOCUS       DAOPOA010000559         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_73133_length_1757_cov_3.505875, whole genome shotgun sequence.
ACCESSION   DAOPOA010000559 DAOPOA010000000
VERSION     DAOPOA010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73133_length_1757_cov_3.505875"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /gene="ptsP"
                     /locus_tag="PKJ17_10410"
     CDS             complement(<1..1245)
                     /gene="ptsP"
                     /locus_tag="PKJ17_10410"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOD76432.1"
                     /translation="MKEEILVNQTLKGTGVSSGITMGRVRLIDRDKVSITKRSISPRQ
                     AENEVGRIRNAVQGAIDQLSHIKDSIPDDDVRKHAFIIDAHILILQDPFFVDSVIDVI
                     RSEHINAEWALDIVVSRFLASFDKVEDPYLRERGQDIRYIYERLARILVVGEKPRISS
                     ETVKGKSIIIAHDLSPADTIQLNLNRVSGFATDVGGRTSHTSIVARALEIPAVVGVGN
                     VTMLVRNNDLVIIDGDEGVVIINPVKEVQKEYAAKQIHLRNQKKEHLRVARLKAETKD
                     GFRMRVGANIELLAEMDIVEKYGAVGIGLYRTEYIYLSRKHDLPSEMDHYHIYRKLAE
                     SKTLQYITIRTLDIGGDKFVSEIEMPKEINPAMGLRAIRLCIKEVPLFKAQLRGILRA
                     SEHGPLRILIPMVSGIEEVRKAK"
     gene            complement(1242..1508)
                     /locus_tag="PKJ17_10415"
     CDS             complement(1242..1508)
                     /locus_tag="PKJ17_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOD76433.1"
                     /translation="MLEGIFTIRNRLGLHARAAAMFVKAAAKFNSIVMVEKDGMEVNG
                     KSIMGLLMLACPLGSKIVLKAEGEDEEKAFRELGGLIDDGFNEE"
     gene            complement(1515..>1757)
                     /locus_tag="PKJ17_10420"
     CDS             complement(1515..>1757)
                     /locus_tag="PKJ17_10420"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76434.1"
                     /translation="TGDGVIILTDMFGGTPSNISLSFLEEGRVEVVAGVNLPMLIKLV
                     TYREGKTLDDLAAFITQYGQKNIYLATDVLKARKKD"
BASE COUNT          295 a          527 c          516 g          419 t
ORIGIN      
        1 cttcgctttg cgcacctcct cgatgcccga gaccatgggg atgaggatgc gaaggggccc
       61 atgctcgctc gccctcagga tccccctcag ttgagccttg aagaggggga cctccttgat
      121 gcagagccgt atggccctga ggcccatggc gggatttatc tccttgggca tctcgatctc
      181 ggagacgaac ttgtcgccgc cgatgtcgag ggtccggatg gtgatgtact ggagtgtttt
      241 gctctccgcc aacttgcgat agatgtggta gtggtccatc tccgagggaa ggtcgtgctt
      301 tcgcgaaagg tagatgtact cggtcctgta gaggcctatg ccgacggccc cgtacttctc
      361 gacgatgtcc atctccgcga gaagctcgat attggcgcct accctcatcc ggaagccgtc
      421 cttcgtctcg gccttgagcc gtgccaccct gaggtgctct ttcttctggt tcctgaggtg
      481 gatctgcttc gcggcgtatt ctttctggac ctccttgacg gggttgatga tgacgacgcc
      541 ctcgtcgccg tctatgatga cgaggtcgtt gttcctgacg agcattgtca cgttgcccac
      601 gccgaccacg gcaggtatct cgagggcccg ggccacgatg gacgtgtggg aggtcctgcc
      661 gcccacgtcg gtcgcgaacc cggagacgcg gttaaggttg agctggatgg tgtcggcagg
      721 cgacaggtcg tgggcgatga tgatggactt gcccttcacc gtctccgagc ttatacgcgg
      781 tttctcaccg acgacgagga tcctcgcgag cctctcgtag atgtacctga tgtcctggcc
      841 cctctccctg aggtaggggt cctccacctt gtcgaagctg gcgaggaacc gggagaccac
      901 gatgtcgagg gcccattccg cgtttatgtg ctcgctgcgg atcacgtcga tgacggaatc
      961 gacgaagaag gggtcctgaa ggatgagtat gtgggcgtcg atgatgaagg cgtgctttct
     1021 cacgtcatcg tcggggatcg aatccttgat gtggctgagc tggtctatcg cgccctggac
     1081 cgcgttcctg atgcggccca cttcgttctc cgcctggcgc ggggatatgg agcgcttcgt
     1141 tatcgacacc ttgtccctgt cgatgaggcg cacccgcccc atggttattc ccgaggaaac
     1201 gccggtcccc ttcagggtct gattgacaag aatctcttcc ttcattcttc gttgaatccg
     1261 tcgtctatga ggcctcccag ttcacgaaag gccttttctt cgtcctcccc ttccgccttg
     1321 aggacgatct tgcttcccag gggacaggca agcatcagaa ggcccatgat gctcttgccg
     1381 ttcacctcca tcccgtcctt ttcgaccatc acgatggagt tgaacttcgc cgccgccttg
     1441 acgaacatcg ccgcggcccg ggcatggagg ccaagcctgt tccttatcgt aaatatccct
     1501 tccagcatcg ctccctaatc cttctttctg gccttcagga catccgtggc gagatatatg
     1561 ttcttctgac cgtactgcgt gatgaaagcg gccagatcgt cgagcgtctt cccctcccgg
     1621 tatgtcacga gcttgatgag catggggagg ttgacgccgg ccaccacctc gacccggccc
     1681 tcttccagga aggaaaggct gatgttggaa ggggtgccgc cgaacatatc agtcaggatg
     1741 atgaccccgt cgcccgt
//
LOCUS       DAOPOA010000560         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_73326_length_1754_cov_3.621542, whole genome shotgun sequence.
ACCESSION   DAOPOA010000560 DAOPOA010000000
VERSION     DAOPOA010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73326_length_1754_cov_3.621542"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="PKJ17_10425"
     CDS             <1..1281
                     /locus_tag="PKJ17_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD76435.1"
                     /translation="YPPEEVERITRVPAGRVREAARLYASAPAACISLGVAVELSTNG
                     FQTARGVAALQAITGNLDIAGGAVFLSEAPLSDLQLTLPKGVKQAIGADEYPLFHRVS
                     RNAQANLYARAILEEKPYPLKGLVVAGSNPVLTWPNAGRVREALSRLEFLAVIDPVMT
                     ATAREAHLVLPCASFLGGDELWDSSHLSLAPRIGLAPRICDDGGLPTHWEIWREIAVR
                     MGHSDFFPWGTEEEAITFRLRTLELTYDDLRDMPEGYTYHRWTAKKYEKEGFNTDSGK
                     VEIYSDLLKRHGYDPVPAYAEPAESPLSTPDVASLFPLVLTTGARRLEYLHSRFRNVD
                     SLRTRVPEPYVEIHPSTADGLGVMDGDKVVVETPRGAVAIKARCTPSILPGVISMSHG
                     WDEANANILTDDACLDPISGFPGGRCLLARIARE"
BASE COUNT          340 a          558 c          523 g          333 t
ORIGIN      
        1 tacccgccgg aggaggtcga gaggatcacc cgggtgccgg cgggacgggt acgggaggcc
       61 gcccggcttt acgcgtcggc accggctgcc tgcatatccc tcggcgtggc cgtcgaattg
      121 agcacaaacg gttttcaaac cgcccgcggc gttgccgcgc ttcaggcgat cacgggcaat
      181 ctcgatatcg ccggcggtgc cgtcttcctc agcgaggcac cgctctcgga cctgcaattg
      241 acgctgccga agggagtgaa acaggccatc ggtgccgatg agtatccgct cttccacagg
      301 gtctcgcgaa acgcgcaggc caatctctac gcccgcgcca tcctggagga gaagccatat
      361 ccgttgaaag gcctcgtggt ggccggaagc aacccggtcc tcacctggcc caatgccggg
      421 cgggttcggg aggcgctgtc aaggcttgag ttcctggccg tcatcgaccc cgtcatgaca
      481 gctacggcaa gagaggccca tctcgtcctg ccctgcgcgt ccttcctcgg cggcgacgag
      541 ttgtgggaca gttcccacct ctctctcgcg ccccggatcg gactcgcccc gaggatatgc
      601 gatgacgggg gattgcccac acactgggag atatggaggg agatcgcggt ccgcatgggc
      661 cattcggatt tctttccctg gggcacggaa gaagaggcca ttaccttcag gcttcggacc
      721 ctggagctca cctatgatga cttgagagac atgccggaag gctacaccta tcatcgctgg
      781 acggcgaaga aatacgaaaa ggaaggcttc aacacggatt cgggcaaggt agagatctat
      841 tcggacctgc tcaagcgcca cggttatgac ccggtccccg cctatgccga acccgccgag
      901 agcccacttt cgaccccgga cgtagcctcc ctgttccccc ttgtgctcac aacaggagcc
      961 cgaaggctcg aatatctcca ttcccgcttc cgcaatgtcg actctcttcg gacccgcgtc
     1021 ccggagccgt acgtcgagat ccatccttcc acggcggacg gtctcggggt gatggatgga
     1081 gacaaggtgg tcgtcgagac gccaaggggg gccgtcgcga tcaaagcgag atgcaccccg
     1141 tcaatactcc ccggcgttat ctcgatgtct cacggctggg atgaggccaa tgccaatata
     1201 ctcaccgacg acgcgtgcct tgaccccatc agcgggttcc ccggcggacg ctgccttctt
     1261 gcccggatcg cccgggaatg acacctcccg accgcccccc atccgcacaa tccttgacat
     1321 tgccacgttc attcagttac attatttcaa aaataaccag aaagactatg tacagaagag
     1381 cgaacatatt cattccattg ggctgagcgg tgcacagggg acccggtggg tttcccgtca
     1441 caaccgtgct cgcggtttcc ccgccttaag tcatcaatcg cctccttgaa gggtccggtg
     1501 cggatgtccg cggccggatc ttttgttttc aggaggtttt catgtcggat tacacacgga
     1561 tgtggtcgga tccgggcctt gacctagtcg aggcattccc ggagaggatg cacgggtgat
     1621 gagggccgcc ggcctcgaca tcggttcacg gaccgtaaag ctcgtggtca ttgagggagg
     1681 cgagacggtc ctcacacgca aggacctcac ctcgcacgac cccctggaag cggcacgcgc
     1741 cctgatgagc gggg
//
LOCUS       DAOPOA010000561         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_73667_length_1748_cov_2.868281, whole genome shotgun sequence.
ACCESSION   DAOPOA010000561 DAOPOA010000000
VERSION     DAOPOA010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73667_length_1748_cov_2.868281"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PKJ17_10430"
     CDS             <1..580
                     /locus_tag="PKJ17_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76436.1"
                     /translation="AWAPFRMIHLLSGTSTVVTRSPFGGPNIAALMEELSAFGVREFV
                     LWGYCGGMGPDRSVGNVCLAGSALREEGISWHYLEDDSDMVSSLWTDDWTHAAAEAGF
                     PVIDIWTTDALYRETRNKIDFCIGRGISAVEMEVASFYAVARAKGLKAVAFLVVSDLF
                     NNNGTWTSGFHTRAFREGVKKLARFMNEKAVL"
     gene            968..1684
                     /locus_tag="PKJ17_10435"
     CDS             968..1684
                     /locus_tag="PKJ17_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOD76437.1"
                     /translation="MTVAGKFVNEKRLDIIANNLANAQTAGFKASRPVFEMISTTQDE
                     AGRQGLLKNAYVNLSDTYIDFSDASIVESGAKLDMAILGPGFFSVMTPEGVQYTRNGQ
                     FMLDKSSRLVTMSGEPVMGKGGEITINVSDGKEILIEGDGSIYLGKDLVDTIKVVEFK
                     DLNGLKPVSKSNFTYTGNEAGETPKLYSIRQGSYETSNVNVVLEMVDLIHTMRAFECY
                     TKIDQMFSDINGKLTELAKF"
BASE COUNT          411 a          477 c          501 g          359 t
ORIGIN      
        1 cgcgtgggct cctttcagaa tgatccatct tctgtccggg acgtcgacgg tagtgacaag
       61 gtctcccttt ggtggcccga atattgccgc attgatggag gaactgtccg cgttcggcgt
      121 cagggagttt gttctctggg gctattgcgg aggcatgggg ccggacaggt cggtggggaa
      181 tgtctgtctg gcgggaagcg cgctccgcga ggaggggata tcctggcatt acctcgaaga
      241 cgacagcgac atggtatcgt cgctctggac ggatgactgg acccatgcgg cggcagaggc
      301 gggatttccc gtcatcgata tctggacgac ggacgccctc taccgggaga cccggaacaa
      361 gatcgacttc tgcatcggac ggggcatatc ggccgtggag atggaagtgg cgtctttcta
      421 cgccgtcgcc agggcaaagg gcctgaaggc cgtcgccttc ctcgtcgtct ccgacctctt
      481 taataacaat ggtacatgga caagcggctt ccacaccaga gccttcaggg aaggcgtaaa
      541 aaagcttgcc cgcttcatga acgaaaaagc ggtcctctga ccgccacacc ccccgggaac
      601 cgcctttttc cctgaaaaaa tttcccaaaa ggaaaatttt tcccagcaaa aggaaaaaag
      661 catcacaggc ctcaggtttc aaagagaata ctgttgcggg ttgcgcgttc cgggttgcgg
      721 gtcaaaaaca ggtgtaaggg ttccgatcct gccggacgac gggaagatcc cccattccag
      781 cctcggacct cgaccccctt cgccggaaca aggcctttca gctgtcaagc gattcaagcc
      841 actcaagcga ttcaaaccgt ctcttcgttc cctgccgcct ttccgttcaa gcggttcaaa
      901 cggctcaagc ggttcaagcg gtcttttctg gcacgtgtat tgcactatcc ccggcatgag
      961 cttttacgtg acagtcgccg gcaagtttgt caatgagaag aggctggaca tcatcgccaa
     1021 caacctggcc aacgctcaaa ccgccgggtt caaggcttcg cggcccgttt tcgagatgat
     1081 ctccacaacg caggacgagg cgggtcggca ggggctcctg aaaaatgcct atgtcaacct
     1141 ttccgacacc tatatcgatt tttctgacgc ttccatagtc gagagtggcg ccaaactgga
     1201 tatggccatc cttggccccg ggtttttctc cgtgatgacg ccggagggtg tccagtacac
     1261 gcgtaacggg cagttcatgc tcgacaagtc gagccgactc gtgaccatga gcggggagcc
     1321 cgtcatgggt aagggcgggg agatcaccat caatgtcagc gatggcaagg agatactcat
     1381 cgagggcgat ggctccatat acctgggaaa ggacctcgtg gacaccatca aggtcgttga
     1441 gttcaaggac ctgaatggtc tgaaacccgt aagcaagagc aacttcacat acaccggaaa
     1501 cgaggcggga gagacgccga aactgtactc catacggcag ggctcctacg agacctcaaa
     1561 cgtcaatgtt gtccttgaaa tggtggacct catccatacc atgagggcat tcgagtgcta
     1621 cacgaagata gaccagatgt tttccgatat caacggcaag ctgacagagc tggcgaaatt
     1681 ctaggaggaa cactatgata cgggcgttat ggacagcggg aacaggcatg aacgtgcagc
     1741 agaccaat
//
LOCUS       DAOPOA010000562         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_73668_length_1748_cov_2.743650, whole genome shotgun sequence.
ACCESSION   DAOPOA010000562 DAOPOA010000000
VERSION     DAOPOA010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73668_length_1748_cov_2.743650"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..855
                     /gene="plsY"
                     /locus_tag="PKJ17_10440"
     CDS             244..855
                     /gene="plsY"
                     /locus_tag="PKJ17_10440"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOD76438.1"
                     /translation="MINFVLNPLLVVFAYFLGSIPVGVMLGRLKGVDPRKTGSGNIGA
                     TNVMRAAGKKLGVVTLILDAAKGFVPVALATLLGVPLCVVALVGLVAFLGHVFPVFLR
                     FKGGKGVATALGVYLAISPVTILAAFVVFVIVAAIWRYVSLASMIGATVVPIGLYLIG
                     APWSFVVMAVLITLVVIVRHKDNITRLVKGTESKLSFSGGSKN"
     gene            complement(1098..>1748)
                     /locus_tag="PKJ17_10445"
     CDS             complement(1098..>1748)
                     /locus_tag="PKJ17_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HOD76439.1"
                     /translation="PHVYPETVRRSVKAAKCTPDDLRIKADGSSRTVRVIKVVNQTIT
                     EEVLWTPPVRNGFLQTDPSADTIRVSVINRYGGTQHGLGFINGMGIKNGAIATTLIWD
                     TGNILTIGSSEEDMAKAVNSLIDIEGGTVIVKDGKVIFEFPMPVFGTMPIGTIDEIAD
                     ITKDMEKKVREIGVTLERPFLTIQTIPFSGLPFLRITDKGLADIKNRRLVPLFVDR"
BASE COUNT          299 a          471 c          548 g          430 t
ORIGIN      
        1 gggttagaaa aatctccgga ttccggcctt cgcgggaatg atggagaaaa tgagttcatc
       61 aacagcctgt tgaacgtcaa acatagagct tctttaccgg cattcgggcg cgcaggcgtg
      121 atatagcggt gtttccgatc tccctttttc ctggattccg accttcgtcg gaatgacagg
      181 gaaagcgaac gttgacagaa cactgttcca tcgattattc tgtcttctgt gctccctgat
      241 cagttgataa acttcgttct gaacccgctg ctggtggtct tcgcctactt tcttgggtcc
      301 attcccgtgg gggtcatgct ggggaggctc aaaggggtcg atcccaggaa gacgggcagc
      361 gggaatatcg gggccacgaa tgtcatgcgc gctgccggca aaaagctggg ggttgtcacg
      421 ctcatcctgg acgcggcgaa ggggttcgtg ccggttgcgc tggcgacgct tctcggcgtt
      481 cctctgtgcg ttgtcgccct tgtagggctc gtggcctttc tgggccatgt tttccccgtt
      541 ttcctgaggt ttaagggagg gaagggcgtt gctaccgcgc ttggggtgta tctcgccatc
      601 agtcccgtga ccatcctggc ggccttcgtc gtcttcgtca tcgtcgccgc catatggcgc
      661 tacgtgtcgc tggcctccat gatcggggcc acagtggttc ccatcggtct gtacctcatc
      721 ggagccccct ggagcttcgt cgtgatggca gtccttatca ccctcgtcgt cattgtccgc
      781 cacaaggaca acatcacccg cctcgtcaag ggcacggaaa gcaagttatc tttctcgggt
      841 gggtcgaaga actagaagct aagagactgc gtcccgcctg cggcgggagg aaaagagttg
      901 tcacattaat gggacccatc agcagacatc gctgctatcg aggttggttt ttcttcccgc
      961 tcggcagagc gggatcgaag atttccggga attcctcaaa ttcccggaaa tgtccttctc
     1021 cgcgatctct cctcatcctc cgtgacctcg agtgagggcc gaaggtccga acgggcgtga
     1081 gattgccgtt tgaaggctta ccgatcgaca aacaacggga caagccgccg gtttttgatg
     1141 tcggccaggc ccttgtcggt gatgcgcaga aaggggaggc cggagaaggg gatggtctgg
     1201 atggtgagga aggggcgttc gagggtgacg cctatctcgc ggaccttttt ctccatgtct
     1261 tttgtgatgt ccgctatctc gtctatggtg ccgatgggca ttgtgccgaa gacgggcatg
     1321 gggaattcga atatcacttt gccgtccttg acgatgaccg tgccgccctc gatgtctatg
     1381 aggctgttga cggcctttgc catgtcttcc tcggagcttc cgatggtcag gatgttgccg
     1441 gtgtcccaga tgagcgttgt cgctatggcg ccgttcttga tgcccatgcc gttgatgaag
     1501 ccgaggccgt gctgcgtgcc gccgtagcgg ttgatgaccg aaacgcggat ggtgtcggcg
     1561 gacgggtccg tctgcaagaa accgttgcgg acggggggtg tccacaggac ctcctcggtg
     1621 atggtctggt tgacgacctt gatgacgcgc acggtcctcg atgacccgtc ggctttgatc
     1681 ctgaggtcgt cgggcgtgca ttttgccgct ttcacgctgc gccgcacggt ctcggggtag
     1741 acgtgggg
//
LOCUS       DAOPOA010000563         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_74127_length_1740_cov_2.910386, whole genome shotgun sequence.
ACCESSION   DAOPOA010000563 DAOPOA010000000
VERSION     DAOPOA010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74127_length_1740_cov_2.910386"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PKJ17_10450"
     CDS             <1..1076
                     /locus_tag="PKJ17_10450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOD76440.1"
                     /translation="FEFFLIHYVKTDRDVYRLVIRNLPMGFSLVDRNGIILEFNHMAQ
                     DLTGYSRDDVIGRSHFEIIHGAQDPGSCPLFARVLKEHTSSVASETLLKTRSGETVAL
                     LVTAFPLFNASGDFIGGVEFFRDISDKKRMERERRNLFSMFAHDMKSPLAGVSGLLQR
                     LFSGKAGPLSDKQRDHLSTIMRSISRLQAMVSKFVEFSRLDTGKCDPVPGPYDINAAL
                     REQIEMVRLTAEKKGVQIVFEDAQVDVPAVEADSAMIDRVLANLLDNAVKYTNAGGTV
                     TVRLTSDARRVFVEVMDTGIGIREQDMPCVFDAFCRVDRDSEGSGLGLSIARGIIEAH
                     NGTLSVESVPGKGSRFWFVIPRR"
     gene            complement(1107..>1740)
                     /locus_tag="PKJ17_10455"
     CDS             complement(1107..>1740)
                     /locus_tag="PKJ17_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOD76441.1"
                     /translation="GVLCNHKNCAANEVHKLGEAGLFEMLAQDNIERFKALGAKKIVT
                     LSPHAYNAMKNLYPLYGGDFQVFHYTELLYELFKEGKLDVSNGFDARLTYHDPCFLGR
                     WNDGYETPRRLLAAVPSLRLAEMEKSRDAALCCGGGAGNFEIDLLGGGDSSPARRRVR
                     HAAETGATVLAVACPKCLVMLEDALKAEELEEKLAVRDIAEILTEAGGIR"
BASE COUNT          345 a          488 c          521 g          386 t
ORIGIN      
        1 aatttgaatt ctttttgata cactatgtca aaactgaccg ggatgtgtac cgcctcgtga
       61 tccggaacct tcccatgggt ttctcgctgg tcgacaggaa cggcattatc ctggagttca
      121 atcacatggc gcaggacctc acgggttatt cccgggacga cgttatcggc agatcacatt
      181 tcgagatcat ccacggcgcg caagaccccg gttcgtgtcc cctgtttgcg cgtgtgctca
      241 aggagcatac ctcttccgtc gcgtctgaga cattgttgaa gacgaggagc ggcgaaacgg
      301 tcgcgctgct cgtcacggcc tttccgcttt tcaacgcctc gggagacttc atagggggag
      361 tcgagttttt cagggacatc tcggacaaaa agcgcatgga gcgggaacgc aggaacctct
      421 tctccatgtt cgctcacgac atgaagagtc cccttgcagg ggtttccgga ctattgcaga
      481 gactgttctc cgggaaggcg ggccctttga gcgacaagca aagggatcac ctttccacca
      541 ttatgagatc catttcgaga ctgcaggcca tggtgtccaa attcgttgaa ttttcaagac
      601 tggacaccgg caagtgcgat cccgtccctg gcccgtacga tatcaatgcg gccctcagag
      661 aacagatcga gatggtgagg ctgacagctg agaagaaagg cgtgcagatc gtttttgaag
      721 acgctcaggt tgacgtgccg gcagtggaag ccgatagcgc gatgatagac agggttctgg
      781 ccaatctcct ggacaacgcc gtgaaataca ccaatgcggg ggggacggtg acggtacgcc
      841 tcacgagcga cgcgcgccgt gtttttgtcg aggtcatgga taccgggatc ggtatccgcg
      901 agcaggacat gccctgtgta ttcgatgctt tttgccgtgt ggaccgtgac agtgaaggtt
      961 ccggtctggg cctgtcaata gccaggggga tcatagaagc ccacaatgga acgctgtcgg
     1021 tcgagagtgt cccgggaaag ggcagcagat tctggtttgt cattcccagg cggtgaagga
     1081 cgggaagacg gacagcgcgg gccccatcat cgtattccgc cggcctctgt cagtatctcc
     1141 gcgatgtccc ttacggccag cttctcctcg agttcctccg ccttgagggc gtcttcgagc
     1201 atgacgaggc atttcggaca agccaccgcg aggacggtcg ctcccgtttc ggcagcgtgt
     1261 ctgacccggc gtcgcgccgg gctcgaatcg ccgccgccga gaaggtctat ctcaaaattg
     1321 ccggcccctc cgccgcagca cagtgccgcg tccctgctct tctccatctc ggccaaccga
     1381 agggagggaa cagcggcgag aagcctcctc ggtgtctcgt acccgtcgtt ccaccgcccc
     1441 aggaagcacg ggtcatggta tgtcagccgc gcgtcgaatc cgtttgaaac gtccagtttt
     1501 ccttccttga agagttcata gagcaattcg gtgtagtgga atacctggaa gtcgccgccg
     1561 tacagggggt agaggttctt catcgcgttg taggcgtggg gtgaaagggt gacgatcttc
     1621 ttcgctccga gggccttgaa gcgctcgatg ttgtcctgcg ccagcatttc gaagaggcct
     1681 gcctcgccca gcttgtgcac ctcgtttgcg gcgcagttct tgtggttgca cagcactccg
//
LOCUS       DAOPOA010000564         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_74330_length_1737_cov_2.429251, whole genome shotgun sequence.
ACCESSION   DAOPOA010000564 DAOPOA010000000
VERSION     DAOPOA010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74330_length_1737_cov_2.429251"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1467
                     /locus_tag="PKJ17_10460"
     CDS             145..1467
                     /locus_tag="PKJ17_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76442.1"
                     /translation="MLTRHTSFKVIATCLLISQALCQTVAWAGPMTLPTIIAPGQSSS
                     AGATSAPSEPSGGAGPTEPDKPLSNIGSGEQTAMAGAGTSETPLKAEASPLDFKISPD
                     GTVATILFDRESVQAMRDLISQLEQTGALGKVRGIVYSAGGFTPMLILMGEKEELHQK
                     LLLLKRFIPDQNQAHMIVISASLRELSDDDAMNVGLTLGPDIIGATISSSAGMLLQSS
                     ESAQYSFSGSADLNAVSFAKIAQLNQALDRSKVLVSSEVYTRNGHKALLTNVQQVPIF
                     STDFNNNVMTSYQQLETSVDVIPTTIDYRKDKPDESQVRVDVLVKISVVTANHTYNAS
                     TTAPEYTTKTFATTRVLKANNARYVVGTFVNDAEYKEQVGLPLLSKIPIIKYLFSREG
                     TRMVRNVAILTLAVRLIPMQVKDLTIDVEHVNPLEELYKRKGSLKDAK"
BASE COUNT          392 a          558 c          484 g          303 t
ORIGIN      
        1 tgcgtggcca ccgcgtatga ggtcaccttc tgcccgtaag gcgacaggtg gtctcgtctc
       61 ggtattgcat ggcatggcaa ccgacaccaa accggcatcg ccatttcgat gccggtttgg
      121 tgatctccga ccaaggaaca tgatttgctg acccggcaca catctttcaa ggtcatcgcg
      181 acctgtctgc tgatctcgca ggccctgtgc cagaccgtcg cctgggcggg gccgatgacg
      241 ctgcccacta tcatcgcacc ggggcagtct tccagcgcgg gcgcgacatc cgctccgtcg
      301 gagccgtcgg gcggggcagg gccgacggag ccggacaagc cgctcagcaa catagggagc
      361 ggcgaacaaa ccgcaatggc gggtgccggc acctccgaaa ctccactgaa agcagaggcg
      421 agccccctcg atttcaagat cagtcccgat ggaaccgtcg ccaccattct gttcgacagg
      481 gagagcgtgc aggcgatgcg tgacctcatc tcgcagctcg aacagaccgg cgccctcggc
      541 aaggtcaggg gtatcgttta ctccgccggc ggcttcactc ccatgctcat cctcatggga
      601 gaaaaggagg aactgcatca aaaactgctc ctcctgaaac gcttcatacc cgaccagaac
      661 caggcgcata tgatcgtcat atcagcgagt ctccgcgaac tgagcgatga cgacgcgatg
      721 aacgtaggtc ttaccctggg tccggatatc atcggcgcga cgatctcgag ctccgccggg
      781 atgctcctgc agtcctcgga gtcggcccag tattccttct cggggtcggc ggacctcaat
      841 gccgtctcct tcgcgaagat cgcccagctc aaccaggccc ttgacaggag caaggtcctc
      901 gtctccagcg aggtctacac ccgcaacggc cacaaggccc tcctgacaaa cgtccagcag
      961 gtccccatct tcagcaccga cttcaacaac aacgtcatga catcctacca acagctcgag
     1021 accagtgtgg acgtcatacc gaccacgatc gactacagga aggacaaacc cgacgaaagc
     1081 caggtccgcg tcgacgtgct cgtgaagatc agcgtcgtga cggcgaacca cacctacaac
     1141 gcgtcgacaa cggcccccga atacacgacg aagacgttcg ccaccacgag ggtcctcaag
     1201 gcgaacaacg cgcggtacgt cgtcggcaca ttcgtgaacg atgccgaata caaggagcag
     1261 gtcggccttc ccctgctctc gaagataccc atcataaaat acctcttttc ccgcgaaggg
     1321 acacggatgg tgcgcaacgt cgccatactc accctcgccg tgcggctcat cccgatgcag
     1381 gtcaaggacc tgacgatcga tgtcgagcac gtcaatcccc tggaagaact ctacaagaga
     1441 aaaggcagcc tgaaggacgc gaagtaagag aagccgttgc gggtttcggg ctgcaagttg
     1501 cgtgtcaaaa gcccgaaagg cgggaagcaa ggaagaggta gttgcggggc aaaagtgggg
     1561 gggtagatag gacctatagg acagaataat aggatatggt gaagttcgca gcgtgcaacc
     1621 gggccgtcct ataggtccta agcgtcctat aggtcttatt ggtcctgtcg aaagccgcgc
     1681 tcccctctct gccctgcccc cgatccctgc ccctgtcttc tgcccccgcc ccttccc
//
LOCUS       DAOPOA010000565         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_74427_length_1735_cov_3.467857, whole genome shotgun sequence.
ACCESSION   DAOPOA010000565 DAOPOA010000000
VERSION     DAOPOA010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74427_length_1735_cov_3.467857"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..515
                     /locus_tag="PKJ17_10465"
     CDS             264..515
                     /locus_tag="PKJ17_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76443.1"
                     /translation="MPWRSFTQNLVKFLNENGSIFLAAVDNGKFGVRPFQLMMEEGGG
                     LYFCTNNTRDNPIFEVFFIANWAATIADFSGNLPEMVAF"
     gene            649..1194
                     /locus_tag="PKJ17_10470"
     CDS             649..1194
                     /locus_tag="PKJ17_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOD76444.1"
                     /translation="MSDQGLIDRSDCLLVVIDVQEKLMPAISGKERVIANTATLVRFC
                     GICGIPVIFTEQKKLGPTVPEVKDLTQGFAAVEKVHFNCFLNGEFREHVDRLGRKTIV
                     LTGAESHICVAQTAIAALPRLNVHIIADAVSSRLPDNRAIALDRMRDAGAVISSTEMF
                     IYEILREAGTEEFKAVLPLVK"
     gene            1307..>1735
                     /locus_tag="PKJ17_10475"
     CDS             1307..>1735
                     /locus_tag="PKJ17_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD76445.1"
                     /translation="MKDTSKTTEGMSAANGKTQGKRDNGGYAPEFRDLYHSFFEFAPE
                     AMGVTDPETGLILDVNKAFEAWSGYTREELLGKSTVELGFWVHEEDRRAIIQRLCETE
                     YVEGIELDWKNAKGDVKRLLFSARLIDTPRGKLVLSIGKDI"
BASE COUNT          384 a          479 c          508 g          364 t
ORIGIN      
        1 cgacgaggaa tttgagatgt ttgtggaccg cctggcggct gatgccgaat tgccccgtcg
       61 tcctcgagac aacgtctctc gaccgatcct ccacatttct caagatgaag gcccgaaccg
      121 cctcgcctct cttcctcgtt cttcccgcca tagtgtaaac cttatgttat ttgtagagta
      181 aactataagg cgtgtatcgg tcaataccaa aatctctccg gtgtccccgg tgacgcaggg
      241 gaacagtcgt ggaggcacga aaaatgccat ggaggtcatt cacgcagaat ctggtgaagt
      301 ttctgaacga gaacggcagc atttttctag ccgccgtcga caacggcaag tttggggtca
      361 gacctttcca gcttatgatg gaggaagggg gcgggttgta tttttgcacc aacaacaccc
      421 gggacaaccc catcttcgag gtctttttca tcgcaaactg ggccgcaacg atcgcggact
      481 tttccggaaa cctcccggag atggtggcct tctgaccgga agaggaaggc ccttccctct
      541 cggcgggacc tgaaaccctc tttacccccg ccttcgaact ggtttatact gtggcgacgg
      601 cgtggaccat gaggccggcc gagggttcgg ggataaaagg aggaagcgat gtctgatcag
      661 ggcctcatcg acaggagcga ctgtcttctc gtggtcatcg acgtgcagga aaagctgatg
      721 ccggcgatct ccgggaagga aagggtgatc gcgaatactg cgacgctggt gaggttctgc
      781 ggcatatgcg ggatccccgt gatcttcacg gagcaaaaga agctggggcc gacggtcccc
      841 gaggtcaaag acctcacgca gggcttcgcc gccgtggaga aggtccattt caattgcttc
      901 cttaacggcg agttccgcga gcatgtggac aggctcggcc gaaagaccat cgtccttacc
      961 ggggccgagt cccacatatg tgtcgcgcag accgccatcg ccgccctgcc ccgcctcaac
     1021 gtccacatta tcgccgatgc cgtgtcttcc aggttgcccg acaacagggc gatcgccctc
     1081 gacaggatgc gtgacgccgg tgccgtcata agttccacgg agatgttcat ctacgagata
     1141 cttcgtgagg ccggcactga agagttcaag gccgtcttgc cccttgtgaa gtgatttttc
     1201 taaaccttgc ctgaaattca tgcgataatc cggtgaagcg acgggttctc gggcggtctc
     1261 tcgatgtcca tgaaagggag cccattttac ctgaggcgga ggcacagtga aggacacctc
     1321 gaagaccacg gaaggtatgt ccgctgcaaa tggaaagaca caggggaaac gggacaacgg
     1381 cgggtatgcg cccgaattcc gtgacctcta ccactccttc ttcgagttcg ctcctgaagc
     1441 catgggagtg accgatcccg aaaccggtct gatcctcgat gtcaacaagg ccttcgaggc
     1501 atggtccggt tacacccgcg aggagcttct ggggaagagt accgtcgagc ttggtttctg
     1561 ggtccacgag gaggacaggc gggcgatcat acaaaggctg tgcgagacgg agtatgtcga
     1621 aggtatcgag cttgactgga aaaacgcgaa gggcgacgta aagaggctcc tcttttccgc
     1681 caggctcatc gatacaccac ggggtaagct cgttctgtcc atcggaaagg acatc
//
LOCUS       DAOPOA010000566         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_74428_length_1735_cov_3.411905, whole genome shotgun sequence.
ACCESSION   DAOPOA010000566 DAOPOA010000000
VERSION     DAOPOA010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74428_length_1735_cov_3.411905"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PKJ17_10480"
     CDS             complement(<1..572)
                     /locus_tag="PKJ17_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOD76446.1"
                     /translation="MYHQGNRGRGKLFSLFFILAVCCLFLGASVLHPSARAEAASVTL
                     GWDAATGVSGYKIHRGTASGSYTTSIDVGNRTSYTLSDLTSGSTYYFAVTAYDTSSEQ
                     SGYSNEVSYAGGSSCTYSISPSSASFTSSGGTGTITVTAASGCTWSASTGVSWATISS
                     GTSGTGSGTVAYSVAANTGSSRTAAFSVAG"
     gene            900..>1735
                     /locus_tag="PKJ17_10485"
     CDS             900..>1735
                     /locus_tag="PKJ17_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOD76447.1"
                     /translation="MRLSIFSRLFLGYIAIILTLGAISAYTVLRLRQVNVEAGIIFNV
                     DQRVVDLKQKLVDSLLSHVGSEKKHVITGDPIFLEQMESGQKTFEGYLSQVSQIVGAS
                     SNRDILARVGSEYARYLSLVNEEIELSKTGRRYSRSEYGHRKEEAVGQVLEALKELEN
                     SYVNDIQERMNRIQETAGSAERLSGLMFAAAVLIVILGSLISTRNITRPLKVLVAKTR
                     EISRGVFEGDLNIGSPREVAELARAVNLMCEKLKKVDTMKSGFFATMSHELRTPLASI
                     KQG"
BASE COUNT          364 a          500 c          510 g          361 t
ORIGIN      
        1 tgtcccgcca ccgaaaaggc cgcggtcctc gatgatcccg tgttggccgc caccgagtag
       61 gccacggtgc cgctccccgt accgctcgtg cccgaggaga tcgtggccca tgacactccc
      121 gtcgacgcgc tccaggtaca accgctcgcg gctgtcaccg tgatggtccc cgtgccgccg
      181 ctgctggtga aggaggcgct tgacggtgaa atggaatacg tgcatgaact cccgcccgca
      241 tagctcacct cgttggagta accgctctgc tcgctcgatg tgtcataggc ggtcacggcg
      301 aagtagtatg tgctgccgct cgtaaggtcg gaaagggtgt aacttgtccg gttcccgaca
      361 tcgatgctcg ttgtgtagct tccactcgct gtccccctgt gtatcttgta tcccgaaacg
      421 cccgtcgccg cgtcccagcc gagtgtcacg cttgctgcct cggccctcgc cgaaggatgg
      481 agcacggacg ctcccaggaa aagacagcac acggcgagaa tgaaaaagag agaaaataac
      541 ttgcctctac cccggttgcc ctggtgatac attgaaacct cctgaacatt taatacgtgc
      601 cggatcgact ggccaattct cgatcctgcc acaggaatag caaaagccgt gcctcggggt
      661 gacgaaatgt gctatactat cgaagttaaa ggtaatgaga tgtttccgca gtttgtctgt
      721 aaggcttcag atgcagggtt ttcccttcat gtcggatctc tgactgaagg atatttcctc
      781 cacacactcc tccccggtca ggctgcatat cgtcccggcg gatcatcagg tacctgattt
      841 agaccggtcg cccgatccgg tgctgaaccc ggcacgggcc cggctggaat gactgtccca
      901 tgagactgag tatattttcc aggctgtttc tcgggtatat cgccattatt cttaccctgg
      961 gggccatcag cgcgtacacg gtcctcaggc ttcgccaggt gaacgtcgag gcgggcatca
     1021 tcttcaatgt cgaccagcgt gttgtggacc tcaaacagaa actcgtcgat tccctgcttt
     1081 cccacgtggg gtcggaaaag aaacatgtca tcaccgggga cccgatcttc ctggaacaga
     1141 tggaatcggg gcagaagacc ttcgaagggt atctgtccca ggtgtcgcag atcgtaggcg
     1201 cctcttcgaa cagggacatc ctggcccggg tggggtcgga gtacgcacga tacctgtcgc
     1261 tggtgaacga ggaaatagaa ctgtcgaaga ccggccggcg gtacagcagg agtgaatacg
     1321 gacatcggaa ggaagaggcc gtggggcagg tgttggaggc gttgaaggag ctcgagaact
     1381 cctacgtcaa tgacattcag gagcggatga acaggataca ggagacagcg ggctcggctg
     1441 agcgcctgtc gggcctgatg tttgccgccg cggtgctgat cgtcatcctg ggttccctca
     1501 tcagcacgag gaacatcacc agacccctga aggtcctggt ggcgaagaca agggagatct
     1561 cccggggcgt cttcgaaggg gacctcaata tcggctctcc ccgtgaggtt gcggaactgg
     1621 ccagggccgt caacctgatg tgcgagaagc tcaagaaggt cgatactatg aagtccggct
     1681 tcttcgccac catgtcccat gaactgcgca ctcctctcgc gtccatcaag caggg
//
LOCUS       DAOPOA010000567         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_74441_length_1735_cov_2.893452, whole genome shotgun sequence.
ACCESSION   DAOPOA010000567 DAOPOA010000000
VERSION     DAOPOA010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74441_length_1735_cov_2.893452"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PKJ17_10490"
     CDS             <1..598
                     /locus_tag="PKJ17_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOD76448.1"
                     /translation="VNLCRKLLEHIGINPKRLRLEWVSAGEGIRFANIMNEFAREIEE
                     LGPLGKSEGLETAELNARIEKMAKLVPYIKLEKMEKLALHDINGDYTKLYTSEEIDGL
                     IREAPSYWIDPDRCQACMTCARRCPADAVISAKNQVHVIDQDKCIRCGTCLAACPERF
                     GAVTKIVGEPVPPPLPEEKRAIVRKGKEGAGESSPVAG"
     gene            624..899
                     /locus_tag="PKJ17_10495"
     CDS             624..899
                     /locus_tag="PKJ17_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOD76449.1"
                     /translation="MSDYINAKGLGCAQPVLLARRSIELHHETTIVVDTRESLENIRV
                     LAMHTGSAMEIAEEPGGIYSITLKRRPDTAGYTRGSEKSSSKGNDGS"
     gene            896..>1735
                     /locus_tag="PKJ17_10500"
     CDS             896..>1735
                     /locus_tag="PKJ17_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD76450.1"
                     /translation="MTYSDQPISRSHGEGPGEFTGSRTVCGICSGTCGMTLRLENGEL
                     SAIEGDSEHPVSRGHICPKGRALPEMFRTPDRLRHPVRKAESGSWEEISWDEAFAVLS
                     EKLDGIRRKYGPEALAVHVGHAGVGKEFLPYAERFCALYGTPNFSTCGSHCYESKSMA
                     NMVTFGKMPIGDYARSKCIVLWGKNPGSSAPSLVNEIAEARRRGCALIVIDPRRTPLA
                     EKADFHLQLRPGTDGALALGFLHVIITEQLYDKAFVERWTVGFDALCQAVEAYPPEEV
                     ERIT"
BASE COUNT          405 a          489 c          561 g          280 t
ORIGIN      
        1 ggtgaacctc tgcaggaagc tcctcgaaca catcggcata aacccgaaga gactgcgcct
       61 cgaatgggtc tccgcaggcg agggcatccg ctttgccaac atcatgaacg agttcgccag
      121 ggagatagag gaactggggc ccctcggaaa gagcgagggg ttggagacgg cggaactcaa
      181 cgcgcgcatc gagaagatgg cgaaactcgt cccctatata aaactggaga agatggagaa
      241 gctcgccctt cacgacataa acggggacta cacgaagctt tacacctctg aggagataga
      301 cggccttata cgggaggcac cgtcgtactg gatagatccg gacaggtgcc aggcgtgcat
      361 gacctgcgct cggagatgcc cggcggacgc tgtcatcagc gcgaagaacc aggtccacgt
      421 gatcgaccag gacaaatgca tacggtgcgg gacgtgcctt gcggcctgtc cggagaggtt
      481 cggcgcggtg acaaagatcg tcggcgaacc cgtccccccg cctcttcccg aggagaagag
      541 ggccatagtc agaaagggga aggaaggtgc cggggagagc agcccggttg ccgggtgaaa
      601 ggcgatccgg gagcgtgccg cacatgtccg actatataaa cgcgaaaggc ctgggttgcg
      661 ctcagccggt cctccttgcg agacgatcca tcgaactgca tcatgagacc acgatcgtcg
      721 tggatacccg ggaatccctg gagaacatcc gggtcctcgc catgcatacg ggatccgcga
      781 tggagatcgc agaggagccg ggaggcatct actcgataac gctgaagagg agaccggaca
      841 ccgctggata cacccgcggg tccgaaaaga gcagcagcaa ggggaacgac gggtcatgac
      901 atattcagat cagccgatat cccgatctca cggcgaaggg ccgggggaat tcaccggcag
      961 ccgcaccgtt tgcggtatct gcagcggcac gtgcggaatg acgctgagat tggagaacgg
     1021 ggagctctcc gctatcgaag gcgattcaga acacccggtg agcaggggac acatttgccc
     1081 caagggacgc gccctgccgg aaatgttcag aacaccggac cgactgaggc atccggtcag
     1141 gaaagcggag tccggctcct gggaagagat atcgtgggac gaggctttcg cggtcctttc
     1201 ggaaaagctg gacgggatac gaaggaaata cggacccgag gcgctggcgg tccacgtggg
     1261 acacgcaggg gtgggaaaag agttcctgcc gtatgccgaa cgtttctgcg ccctctacgg
     1321 cacgcccaat ttctcgacct gcggcagcca ttgttacgaa tcaaaatcga tggccaacat
     1381 ggtcaccttc ggcaagatgc ccatcggtga ttacgcgcgg agcaaatgca ttgtcctgtg
     1441 ggggaagaac ccgggctcat ccgccccctc cctcgtcaac gagatcgccg aggcaagaag
     1501 gcgggggtgc gctctcatcg tcatagaccc gagaaggacg ccccttgccg aaaaggcgga
     1561 cttccatctc cagctgaggc cgggcacgga cggcgccctg gccctgggct tccttcacgt
     1621 gatcatcacg gagcagctct acgataaggc ttttgtcgaa agatggacgg tcggttttga
     1681 cgctctctgt caggctgtcg aggcctaccc gccggaggag gtcgagagga tcacc
//
LOCUS       DAOPOA010000568         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_74528_length_1734_cov_2.453246, whole genome shotgun sequence.
ACCESSION   DAOPOA010000568 DAOPOA010000000
VERSION     DAOPOA010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74528_length_1734_cov_2.453246"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PKJ17_10505"
     CDS             <1..943
                     /locus_tag="PKJ17_10505"
                     /inference="COORDINATES: protein motif:HMM:NF015694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOD76451.1"
                     /translation="KEGIPILLHTHLANDAAETLLKRGLVPGLEDARILRREATFGDS
                     RFDFLLEKDGREMVLEVKNCTLFGRTLAMFPDAVTERGSRHLEGLMRLASSGMPAGVL
                     FLVQWPHARYFMPEYHTDLLFSRTFARCRDRILVEALALNWRADLSLDGAVRKLTIPW
                     DTIERECRDRGCYILLLKLDEDTRLRTGGLGDILYRKGYYLYAGSAKKGLTKRMERHA
                     RTRKKLFWHIDYLREKASFHKVLAIRTDKDIECVLAAGLSRISRWSVPGFGCSDCSCR
                     SHLFGMDTDPMDSPDLIRLVYDHRMGSIERELRSGHD"
     gene            936..>1734
                     /locus_tag="PKJ17_10510"
     CDS             936..>1734
                     /locus_tag="PKJ17_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76452.1"
                     /translation="MIDVHTHGLGGYETRGASPGDILAMARLQGSAGVEAFLPTIFPA
                     PIEEMRRDIFAVRAAMLMQREEGESLGVSSVARITGVHLEGPFLNPAEAGALDRGSFL
                     PATADTWRKLVEGFEDIVRVITIAPELDGARELIRDMATMGVVVSMGHSDATRSEAEA
                     GFRAGARGVTHIFNAMRGFHHREPGIAGFALMSPDVYVEVIADPYHLDLKTIELIFRL
                     KDPGRIILVSDTVKGSPCSNEGRPVTDDEGKLLGGSMPLPAATRRLMA"
BASE COUNT          345 a          520 c          535 g          334 t
ORIGIN      
        1 gaaggaaggt attcccatcc tccttcacac ccatctcgcg aatgacgcgg cggaaacgct
       61 cctcaaaaga ggtcttgtcc ccggactcga ggatgcccgc atcctcaggc gggaggcgac
      121 gttcggcgac agcaggttcg attttctcct tgagaaggat ggccgggaga tggtgctgga
      181 ggtgaagaac tgcacccttt tcggccgcac ccttgccatg ttccccgacg cggtcacaga
      241 gcgcggaagc cggcacctcg aagggctcat gcggcttgcg tcgtccggca tgcccgcggg
      301 cgtgctcttt ctcgtccaat ggccgcacgc gcggtacttc atgcccgaat accacacgga
      361 ccttctcttt tcacggacct tcgcgaggtg cagggaccgc atcctcgtcg aggccctcgc
      421 ccttaactgg agggccgacc tgtccctcga tggcgcggtg aggaagctca ctatcccctg
      481 ggacacgatc gaaagagagt gccgcgacag gggatgctac atactgctac tgaaactgga
      541 cgaggacacc cggttgagga ccggcggtct gggagacatc ctttacagga agggttatta
      601 tctctatgcc ggttcggcga aaaaagggct cacaaagaga atggagcgcc acgccagaac
      661 acgcaagaaa cttttctggc acatcgacta cctccgggag aaggcgtctt tccacaaggt
      721 cctggccata aggaccgaca aggacataga gtgcgttctt gccgccggac tcagccgtat
      781 atcccggtgg tccgttcccg gtttcgggtg ctccgactgt tcatgcagga gtcatctctt
      841 cggcatggat acggacccca tggattctcc cgatcttatc cgtctcgtat atgaccaccg
      901 catgggttcc atcgaaagag agcttcggag tgggcatgat tgacgtgcat acacacggtc
      961 tcggtggata cgaaacacgc ggggcgagcc ccggggacat cctcgctatg gcacggctgc
     1021 agggatcggc gggtgtggag gcgttcctgc ccacgatctt tccggcgccc atcgaggaaa
     1081 tgcgccgcga catctttgcc gtcagggcgg ccatgctcat gcagcgcgaa gagggggaaa
     1141 gccttggagt ctcttcggtg gcgcggatca cgggtgttca tctcgaaggg ccctttctca
     1201 atccggctga ggcgggcgcc cttgacaggg gatccttcct ccctgcgacc gcggatacgt
     1261 ggaggaaact cgtggagggg ttcgaggaca tcgtgagggt catcaccatc gcccccgagc
     1321 tcgacggcgc gagagaactt atcagggaca tggcgacaat gggggtcgtc gtcagcatgg
     1381 gccattcgga cgctacccgg agtgaggccg aggcgggatt ccgggcgggt gcccggggcg
     1441 tcacccatat cttcaacgcc atgcgcggct tccaccatcg cgaaccgggc atagccggtt
     1501 ttgctctcat gagccccgat gtctacgtgg aggtcatagc cgacccgtat catctggatc
     1561 tgaagacgat cgaactcatc ttcaggctga aggaccccgg ccgcatcatt ctggtttccg
     1621 ataccgtgaa gggatcgcca tgctccaacg aaggaagacc ggtcacggat gacgaaggaa
     1681 aacttctcgg aggttccatg cccctgccgg ccgcgacaag gcgcctcatg gccc
//
LOCUS       DAOPOA010000569         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_74734_length_1731_cov_2.073986, whole genome shotgun sequence.
ACCESSION   DAOPOA010000569 DAOPOA010000000
VERSION     DAOPOA010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74734_length_1731_cov_2.073986"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="PKJ17_10515"
     CDS             complement(<1..1533)
                     /locus_tag="PKJ17_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOD76453.1"
                     /translation="MAKKHPDHKLHTACIVACVYDNVETVRSRLSQGTDPNTKDEAGQ
                     TPLHYVCRFRGPLAIVRLLLDAGADPNVRDMAGYTPFHWACISGRAAIVRLLLDKGAD
                     VNAQDNEDDTPLSWIKLARSSLSTCERLIDRSDKHSIPLGLPGDWKKWLAEGGQDRKD
                     ASGLSELLTSFSDEWFPSIAAGSDGKGGPSGSGSLAGERSLEKMKDEFNFFRTAQDRK
                     RFFDVAERRYATVSLTQFSWEQWKKVILIYVKMLVSTGNARREVLDGVRSFLSSVPYD
                     QQKSPIVYDALKRMPYGIVSDFNHDVKSVMTLLDRDLFGLEDAKKQVFYELVLAAHSK
                     EASTPAPLLLVGPPGTGKTAMAQAVASAIGLPFHKVSFSGNSDIITMKGSNYGWSNAS
                     MGVFTRILLSAGCLNPVILLDEVDKAGGYNNGNTIDVLTEVLDANQSWNFSDQFLAGV
                     PVDLSRAFFIATANDVDAVPGYIRDRCRVINIPQYSESERKTIIRDFMPGQVRSGRSL
                     PFT"
     gene            complement(1551..>1731)
                     /locus_tag="PKJ17_10520"
     CDS             complement(1551..>1731)
                     /locus_tag="PKJ17_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76454.1"
                     /translation="LKAGANPWIGNRDEILPIDIVRSKDEGPEREDIERVFDRDGGVN
                     ESTLGYYREKYPVSV"
BASE COUNT          364 a          502 c          471 g          394 t
ORIGIN      
        1 ggtgaaggga agcgatctcc cggagcgcac ctgccccggc atgaaatccc tgatgatcgt
       61 tttccgttcg ctttcgctgt actgagggat gttgataaca cggcacctgt cacgaatata
      121 tccggggacc gcatcaacgt cattcgcggt ggcgatgaag aacgctctcg acaggtccac
      181 cgggacaccc gccagaaact ggtcggagaa attccacgac tggttcgcat caagcacctc
      241 ggtgagaacg tcgattgtgt tgccattgtt gtatcctccg gccttatcga cctcgtcgag
      301 gaggatcaca ggattcaggc aaccggcaga gaggaggatc ctggtgaaca cccccatgct
      361 ggcgttgctc cagccgtaat tgcttccctt catggtaata atgtcggaat tcccggaaaa
      421 gctgaccttg tgaaagggga gcccgatcgc cgacgccacg gcctgggcca tggctgtctt
      481 gcccgtaccc gggggcccaa cgagcagcag gggtgccggc gtggaggctt cctttgaatg
      541 tgcggcaagg accagctcat agaatacctg tttcttcgca tcttccagtc cgaagagatc
      601 cctgtcgagc aacgtcatga cgctctttac gtcatgattg aaatcgctga cgatcccata
      661 gggcatcctt ttgagagcat cgtacacaat ggggcttttc tgctggtcat agggcacgga
      721 tgacaggaag ctcctgacgc cgtccaatac ctcccggcgg gcgttccccg tggaaacaag
      781 catcttcaca tatattagga tgaccttttt ccactgttcc caggagaatt gtgtcaatga
      841 cacagtagca tagcgcctct cggcaacatc aaagaacctc ttccggtcct gtgctgtgcg
      901 aaagaagttg aactcgtctt tcatcttttc cagcgaccgt tcacctgcca atgaaccgct
      961 cccggacgga ccacctttcc cgtcgcttcc cgcagcgatc gaagggaacc attcatccga
     1021 gaaggatgtc agtagttcgg acaggccgct ggcatccttc cggtcttgcc cgccctctgc
     1081 cagccacttt ttccaatcgc caggcagacc caaggggatg ctgtgtttgt ctgaacggtc
     1141 aatcaaccgt tcgcaggtag aaagagacga cctggcaagt ttgatccagc taagaggtgt
     1201 gtcatcttca ttgtcttgtg cgttaacgtc ggctcccttg tcaaggagga ggcggacgat
     1261 ggcggcgcgg ccgcttatgc acgcccagtg gaagggggtg tagccggcca tgtcacgtac
     1321 attcggatcc gcaccggcgt cgaggaggag acggacgatg gcaagaggac cacggaaacg
     1381 gcagacataa tgaaggggcg tctggccagc ctcgtccttc gtgttcggat ccgtaccctg
     1441 actgaggcgg gaacggacgg tctcgacatt atcatagacg catgcaacaa tacatgcagt
     1501 gtggagctta tggtcaggat gttttttcgc cacgaatgtc tctctttccc tcatacactg
     1561 acagggtatt tctccctgta gtatccgagc gtcgactcgt tcacaccacc gtcccggtcg
     1621 aacacgcgct cgatatcttc cctctcgggc ccctcatcct tgctacgtac gatgtctatc
     1681 gggagtatct cgtccctgtt ccctatccac ggattcgccc cggccttgag g
//
LOCUS       DAOPOA010000570         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_74963_length_1727_cov_3.412081, whole genome shotgun sequence.
ACCESSION   DAOPOA010000570 DAOPOA010000000
VERSION     DAOPOA010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74963_length_1727_cov_3.412081"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKJ17_10525"
     CDS             <1..219
                     /locus_tag="PKJ17_10525"
                     /inference="COORDINATES: protein motif:HMM:NF023785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76455.1"
                     /translation="RIGEDHDLRDRVKAFEKALEWGDRIPIGVIYRNDRPSFEKRIPV
                     IEGGPLVDGSGRWQELKERLSGSLKEFY"
     gene            251..682
                     /locus_tag="PKJ17_10530"
                     /pseudo
     CDS             251..682
                     /locus_tag="PKJ17_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            776..940
                     /locus_tag="PKJ17_10535"
     CDS             776..940
                     /locus_tag="PKJ17_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOD76456.1"
                     /translation="METGKYVCNACGWVYDPAEGVPQIGIAPVTRFEDLPETFTCPIC
                     NASKGEFSKV"
     gene            953..>1727
                     /locus_tag="PKJ17_10540"
     CDS             953..>1727
                     /locus_tag="PKJ17_10540"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOD76457.1"
                     /translation="MTDILRETDFETLGKVRKGKVRDIYDLGEQLLIVATDRLSAFDV
                     VLPTGIEDKGKVLTKLSLFWFRQMEDIIPNHIIETDVDRYPAALRKYGDALRDRSMLV
                     RKAKVLPVECVVRGYLAGSGWSEYRKSGTVCGITLPEGLKESSKLEKPVFTPSTKADE
                     GHDLNISFEEAVKIIGDDYARKVRDTSIAIYEKARAIGEKRGIIVADTKFEFGVIEGN
                     VLLIDEVLTPDSSRFWSLKDYAPGKSQDSYDKQIVRDYLN"
BASE COUNT          439 a          457 c          514 g          317 t
ORIGIN      
        1 cggatcgggg aggaccacga tctccgtgac agggtcaagg ctttcgaaaa agcattggag
       61 tggggtgaca ggatacccat cggcgtgata tacagaaatg accgtccctc cttcgagaag
      121 aggatccccg tcatagaagg cgggcccctc gtggacgggt caggcagatg gcaggagctg
      181 aaggaaagac tgtcaggatc tctcaaagaa ttctattgaa gccaacatag cgtactgaaa
      241 ggagaacgga atgaatctca aggcaattca gaagatctgc tacggcgtct acatcatcag
      301 ttcgaagaac aatggaaagc ttaacgggca gatagccaac actgccttcc agatcgcgtc
      361 ggaaccgccg accctggcga tcagcatcaa caaacagaac ctgacccacg aatacataac
      421 gtcgagcaag gtgttcacgg tatcgatcct ttccaagttc gctcccatga cgcttatcgg
      481 caagtttggg ttcaagtcgg gcagggacat cgataaattc gctgataccg gacacaggat
      541 gggaacaacg ggcgcgccca tcgtgctcga caacaccgtg ggttacctcg aatgtgaggt
      601 cctgagcatg accgacgcgg gtactcacac ggtgttcatc gggaaggtca ccgactgcga
      661 tgtcctgagc gatgcggagc ccatgaccta tgcgtactac cacacggtca agggaggaaa
      721 gtctccgaag gctgccccca cgtacgtgca ggaagactca cggcctgcgg gtccggtgga
      781 gacggggaag tacgtgtgta acgcctgcgg ctgggtctac gatcccgctg aaggagtacc
      841 ccagataggg atcgctccgg tcacacgctt tgaggacctt cccgagacgt tcacctgccc
      901 gatctgcaac gcttcgaagg gcgaattctc aaaggtatag agaggggctg acatgacgga
      961 catattgcgg gagacagact tcgagacatt gggtaaagtg aggaagggca aggtgcgcga
     1021 catatacgac cttggcgagc agctgctcat cgtggccacc gacaggcttt cagcctttga
     1081 tgtcgttctg cccacaggca tcgaggacaa ggggaaggtt ttgacaaagc tctctctctt
     1141 ctggttcaga cagatggagg acatcatccc caaccatatc atagagacgg atgtggacag
     1201 atatccggcg gccctcagga agtacggcga cgctctcagg gaccggagca tgcttgtcag
     1261 gaaggcgaag gtcctgcccg tggagtgcgt tgtcaggggg tatctggcgg gttccggctg
     1321 gagtgagtac aggaagtcgg gaaccgtctg cggcatcacg ctaccggaag gcctgaagga
     1381 gtcctccaag ctcgaaaagc ctgtattcac gccctcgacg aaggcggacg aagggcacga
     1441 cctcaacatt tcctttgaag aggcggtgaa gatcataggc gacgactacg cgaggaaggt
     1501 gcgggacacg agcatcgcca tctatgaaaa ggcgcgggcc attggcgaga agagaggcat
     1561 catagttgcc gataccaagt tcgaattcgg cgtcatcgag ggcaacgtcc tcctcatcga
     1621 cgaggtcctc acacccgatt catcccgttt ctggtccctc aaagactatg cccccggcaa
     1681 gtcccaggac agctacgaca agcagatagt cagggactac ctcaaca
//
LOCUS       DAOPOA010000571         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_74967_length_1727_cov_3.117823, whole genome shotgun sequence.
ACCESSION   DAOPOA010000571 DAOPOA010000000
VERSION     DAOPOA010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74967_length_1727_cov_3.117823"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PKJ17_10545"
     CDS             complement(<1..538)
                     /locus_tag="PKJ17_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76458.1"
                     /translation="MDGLKFVGQRIPKKDAPLKATGEALYIQDIKVPGMLYGKILYSK
                     YPHARLLKVDAAKALALPGVKAVLTGADVPNNFKFGFLKDNPPLKSGKVLSTRDEVAA
                     VAAISPEIAEQALSLIEVEYEELPGIFDPLEAMKEGAALIHEEFKSNVLKMPWKFVHG
                     DVEAARKESAFIAEDSFST"
     gene            complement(541..1008)
                     /locus_tag="PKJ17_10550"
     CDS             complement(541..1008)
                     /locus_tag="PKJ17_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOD76459.1"
                     /translation="MKKEITFTLNNEKIMVEADPKWTLLYLLRDVLEMTGTKEGCGYG
                     ECGACTVIIDGKAVNSCLYPVMEAEGRNIVTIEGLMSRDGELDPLQKAFIDEGAVQCG
                     FCIPGMIMSAKALLDGKKDPTDEDIKESIEGNLCRCTGYVKIIDAIKSVAGGR"
     gene            complement(1019..>1727)
                     /locus_tag="PKJ17_10555"
     CDS             complement(1019..>1727)
                     /locus_tag="PKJ17_10555"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOD76460.1"
                     /translation="LADATKKVGSLQIRNVATMGGNICNAAPSADTACPLLVLDASAR
                     IAGKGGERVVPLDEFFLGPNKVALEKGEILKGFAMPAFGEGTGSAYIKHTRREAMDLP
                     MLGVAARVTIRVEGSEVGCRDAMCTIDTISNILKRFEDEGLVCEDARIAMGVVAPRPM
                     RAKKAEEALKGKVISEKLLGEIGEIAASEAQPRDSIRGEAWYRRDMIQVLTGRAILRA
                     VDRVIRPDDVIWPQRLW"
BASE COUNT          293 a          581 c          444 g          409 t
ORIGIN      
        1 gggtgctgaa actgtcctcg gcgatgaagg ccgattcctt ccttgccgcc tcgacatcgc
       61 catgaacgaa cttccagggc atcttgagga cgttcgactt gaactcttca tggatgaggg
      121 ccgccccttc cttcatggcc tccaggggat cgaagatccc cggcagttcc tcgtattcca
      181 cctcgatgag gctgagggcc tgctccgcta tctcgggact tatggcggcg acggccgcta
      241 cctcgtcacg cgtcgacaag acctttcccg acttgagggg aggattgtcc ttcagaaaac
      301 cgaacttgaa gttgttcggc acatcggcac cggtaagcac cgccttgacc ccggggagtg
      361 ccagcgcctt cgcggcatcc accttcagca gccttgcgtg aggatatttg ctgtacagga
      421 tcttgccgta gagcatgccc gggaccttga tgtcctgtat gtagagcgcc tctcccgtgg
      481 ccttgagcgg cgcgtccttc ttcggtatcc tttgtccaac gaatttcagg ccgtccatgg
      541 tcacctccca cccgcaacgg acttgatggc atcaatgatc ttcacgtagc cggtgcacct
      601 gcagagatta ccttcgatgc tctccttgat gtcctcatcc gtcgggtcct tcttcccgtc
      661 caggagggcc ttcgcggaca ttatcatgcc gggtatgcag aacccgcatt gaaccgcgcc
      721 ttcgtcgatg aaagccttct ggagagggtc gagttcccca tccctgctca tcagcccttc
      781 tatcgtcacg atgttcctgc cttcggcctc catgacggga tagagacacg aattgaccgc
      841 cttgccgtcg atgatcaccg tgcaggcccc gcactctccg taaccgcacc cttcttttgt
      901 tccggtcatc tcgagcacgt ctcgcagcaa gtaaagaagc gtccacttgg gatccgcttc
      961 gaccattatt ttttcgttgt tcaatgtgaa cgtgatctct ttcttcatga cccaccccct
     1021 accagagtcg ttgcggccat atcacgtcgt ccggccttat gacacggtcg accgccctga
     1081 ggatggcacg gccggtgagg acctgtatca tgtcccggcg ataccatgcc tctccccgta
     1141 tgctgtccct gggctgcgcc tcggacgcgg cgatctctcc tatctctccc aggagcttct
     1201 ccgatatcac ctttcccttc aaggcctcct cggctttctt cgccctcatc ggcctcgggg
     1261 ccacaacgcc catggctatg cgcgcatctt cgcaaacaag cccttcatcc tcaaaccttt
     1321 tcagaatatt cgagatagtg tcgatcgtgc acatggcgtc cctgcagccc acttcgctcc
     1381 cttcgaccct aatggtcacc cttgcggcga ccccgagcat cggcaggtcc atggcttccc
     1441 gcctcgtgtg ctttatgtac gccgaaccgg tcccctcgcc aaaggcgggc atggcgaagc
     1501 ccttcagtat ctcgcccttc tcgagggcca ccttgttcgg tccgaggaag aactcgtcga
     1561 ggggaacgac gcgttcccct cccttccctg ctatccgcgc gctcgcgtca aggacaagga
     1621 gcgggcatgc cgtatcggcc gacggcgccg cgttgcatat gttgcccccc atggtcgcaa
     1681 cgttgcgtat ctgcagggag ccgacctttt tggtcgcgtc ggccagg
//
LOCUS       DAOPOA010000572         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_75047_length_1726_cov_2.612208, whole genome shotgun sequence.
ACCESSION   DAOPOA010000572 DAOPOA010000000
VERSION     DAOPOA010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75047_length_1726_cov_2.612208"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PKJ17_10560"
     CDS             complement(<1..965)
                     /locus_tag="PKJ17_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOD76461.1"
                     /translation="MESAVRLDHNHELTVKDLPYIVTWREDRCKRCGRCTAVCPMHAI
                     EPSVMVRREISSEDASPSPKVTRKTVVVVRQTTDIERYCTGCGSCTLVCPNDAIEPVF
                     NPQHKFLFHKNPGGHPYRRGGRRNDPRESTLDHLKFTRISMLTDPALDAGRHEFRIRT
                     YLGRILPPEEMPFRVKEGKLELMSGNSRFVPPVREIYPVMIGSMSVGALSPTMWEGLA
                     MGVAYLNEVDRMPVVMASGEGGIPPRLLKSRFIKYFILQIASGYFGWDEIIRAIPDMA
                     DDPCAIEIKYGQGAKPGDGGLLMAEKVTKLISEIRGVPQGVDLPSP"
     gene            complement(926..>1726)
                     /locus_tag="PKJ17_10565"
     CDS             complement(926..>1726)
                     /locus_tag="PKJ17_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76462.1"
                     /translation="DRSQEEKEELLIETRLTLRCMGEENGSITVFSFYPDIVTIKEVG
                     DPLQLGEFFGLSDSRLKAKIIFAQGRQNTNYDINLYACHPFFIQGFGSMTNGENTAFV
                     PIREFLASRGFAGYTGYDSDSEVFTHILHYAVKKLGYPLHYYKDVITPLGEGDLWRRE
                     DSEVLSLMRVALRPLCIDGPNVIIGFTPDGSCFMVHDAKKLRPGVVGGVRGRVGLVSE
                     ECGLESMVPERESAKDIFPMKYDMVIVSPGAQEVRVWNQLYGWTTIMN"
BASE COUNT          322 a          538 c          512 g          354 t
ORIGIN      
        1 ggggatggca ggtccacgcc ctgcggcaca ccccgtatct ccgatatgag cttcgtcacc
       61 ttctccgcca tgaggagccc gccgtcaccg ggcttcgccc cctggccgta cttgatctct
      121 atcgcgcagg gatcgtcggc catgtccggg atggcccgga tgatctcgtc ccatccgaag
      181 tagcccgagg ctatctggag gatgaagtac ttgatgaatc tcgacttgag gagcctcggc
      241 gggatgccgc cctcaccgga cgccatgacg acgggcatgc ggtcgacctc gttaaggtac
      301 gcgaccccca tcgccagccc ttcccacata gttggcgaca gggcgccgac ggacatgctg
      361 ccgatcatga cggggtaaat ctcgcgcacg ggcgggacga accgggaatt cccgctcata
      421 agctccagct ttccttcctt taccctgaag ggcatctctt cgggaggcag aatgcgcccg
      481 agataggtgc gtatcctgaa ctcgtggcgg ccggcatcga gcgccgggtc cgtgagcatc
      541 gatatacggg tgaacttcag gtggtccagc gtcgattccc gggggtcgtt ccgcctgccg
      601 cccctgcggt acgggtgtcc tcccgggttc ttgtggaaga ggaacttgtg ctgggggttg
      661 aaaacgggtt ctatggcatc gttggggcac acgagggtgc agctgccgca acccgtgcag
      721 tagcgctcta tgtccgtggt ctgcctgacc accacaaccg ttttccgcgt gaccttcgga
      781 gaaggagatg cgtcttcgga gcttatctcg cgcctcacca tcaccgaggg ctcgatggcg
      841 tgcatgggac agacggcggt gcaccggccg catctcttgc acctgtcctc gcgccacgtg
      901 acgatatagg gaaggtcctt cactgtcagt tcatgattgt ggtccagccg tacagctgat
      961 tccatacccg cacctcctgc gctcccggtg aaacgatgac catgtcatat ttcatgggga
     1021 agatatcttt ggcactctct ctttcgggaa ccatgctttc gagcccgcac tcctccgaca
     1081 ctagtcctac cctgccccta acgccgccca caaccccggg gcggagcttc ttcgcgtcgt
     1141 ggaccatgaa acaggagccg tcgggggtga acccgatgat gacgttcggc ccgtcgatac
     1201 acagcgggcg aagggccacc cgcatgaggg aaagcacctc gctgtcttcg cgccgccaga
     1261 ggtcaccctc gcccagaggc gtgatgacgt ccttgtaata gtggagggga tagccgagct
     1321 tcttcaccgc gtagtggagg atgtgcgtaa agacctcgct gtcgctgtcg taccccgtgt
     1381 accccgcaaa ccccctgctc gcaaggaact ccctgatagg cacgaaggcc gtgttctcgc
     1441 cgttggtcat ggaaccgaaa ccctggatga agaaaggatg gcaggcatag aggttgatgt
     1501 cgtaattcgt gttctgccgt ccctgggcaa agatgatctt cgccttcagg cgggaatcgg
     1561 agaggccgaa gaactccccg agctgcaggg gatcccccac ctccttgatg gtgacgatat
     1621 ccgggtaaaa ggagaagacc gttatggaac cgttctcctc ccccatacac cgaagggtga
     1681 gccgcgtctc gatgagaagc tcttccttct cttcctgact ccggtc
//
LOCUS       DAOPOA010000573         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_75098_length_1725_cov_2.715569, whole genome shotgun sequence.
ACCESSION   DAOPOA010000573 DAOPOA010000000
VERSION     DAOPOA010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75098_length_1725_cov_2.715569"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(593..1237)
                     /locus_tag="PKJ17_10570"
     CDS             complement(593..1237)
                     /locus_tag="PKJ17_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOD76463.1"
                     /translation="MSGTWGVIVIDMQGDFTEWKRGSLAVPGSDEVYARNVESVTRQL
                     KDLGIPILGTQDWHPPDHVSFATSHPGRKPFDTVVIDGETQVLWPPHCVQGTENARIV
                     IDNNLFLAVVKKAQDPSTESYSAFQAGGELERILQWNGIDRVIVYGLATDYCVRATSL
                     DLTAAGYLVVVIEGLCRSVAPDTAAAAVDEMRSKGVRVVATPGEAIEEVRRNSG"
     gene            complement(1289..>1725)
                     /locus_tag="PKJ17_10575"
     CDS             complement(1289..>1725)
                     /locus_tag="PKJ17_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOD76464.1"
                     /translation="YNGYITDETRSFVVGDLAEPFRKPYETARSIIEDVLSFAGPGTD
                     CRDIFHRAEGMARKAGLSDLFMGHGEGKVAFIGHGLGLEINELPVLTPRHERILKEGM
                     VFAFEPKFVLPDLGAVGMEIDLIVGSERLERVTTGPFDLVCL"
BASE COUNT          370 a          522 c          483 g          350 t
ORIGIN      
        1 gataccggga aatggccttg accgccggct ctccgatata cccgaaggcc gcctggacat
       61 cctgcaggat tgggatcaac tcatcgggac tgcggccatg cttcccgagg atccttcgaa
      121 tttgttcggg catggatctc tcctatgcca cattcgtgtg gcgttaactg agatcagtcg
      181 cgccgcgggt cgttcgaaca ggtaagatga gatctagaca aaggtcacgt gattgttgaa
      241 tcagcagacg atcacttccc gaatttgccc ccatgtactc tcattattaa agaacgttca
      301 aattcgtgtc aagaaaaatt ccataaaaaa aatggaccac aagcaattac cgcaagttag
      361 ccgcgaggcg ccaaacggcc cccggggcgc tcccgcccgg ggtccgggaa agaagacgtg
      421 agcatgcggt ctggatacag tataccgttt tgttgatacg gaggaccgac ccgctcgccc
      481 ctccggcctg gtcctgtgac gggaagggcc gtgcggcaat accggatggg atgaagtatg
      541 gattgatgga ccggtgatcg cgggccgcag ccgggtggaa tccagggatc gatcaaccgc
      601 tgttcctgcg gacctcttcg atcgcctctc ccggtgtggc cacaacacgc acccctttgc
      661 tcctcatctc gtcgacagcg gccgcggccg tgtcgggagc cacggaccgg caaaggcctt
      721 ctatgacgac caccaggtaa ccggcagcgg tgaggtcaag ggacgtggcc ctgacgcagt
      781 agtcggtcgc gagtccgtaa actatcaccc tgtctatccc gttccactgc agaatacgct
      841 ccaactcgcc cccggcttga aatgccgaat agctttcggt cgagggatcc tgagccttct
      901 tcaccacggc aagaaagaga ttgttgtcta tgacaatgcg cgcgttctct gttccctgaa
      961 cgcaatgggg aggccagagg acctgcgttt ccccgtcgat gaccaccgtg tcgaagggct
     1021 tcctccccgg gtggctcgtc gcgaaggaaa cgtgatcggg aggatgccag tcctgggtgc
     1081 cgagaatggg gatgccgaga tctttgagct gccgcgtgac actctcgaca tttctcgcgt
     1141 acacctcatc ggatccggga acggcaaggc tgccccgttt ccactccgtg aagtcgcctt
     1201 gcatgtcgat gactatgaca ccccatgtgc cagacatatc tctactctta ccacacgggt
     1261 cgagatccgt caaaggacgt gaaggcgatt aaaggcacac gaggtcgaag ggacctgtcg
     1321 tgaccctctc gagacgctcc gaaccaacga tgagatcgat ctccataccc accgctccga
     1381 ggtcgggaag cacgaacttc ggctcgaagg caaacaccat cccttccttc agtatccgtt
     1441 cgtggcgcgg cgttagaaca ggcaattcgt tgatctcgag accgaggccg tggccgatga
     1501 aagccacctt gccttcaccg tgccccatga agaggtcgct gaggccagct ttgcgtgcca
     1561 tgccttcggc gcggtgaaag atgtcccggc aatcggtccc cggccccgca aaagacagca
     1621 catcctctat gatcgaccgt gccgtctcat agggctttct gaaaggctcc gccaggtcgc
     1681 ccaccacaaa gctcctggtc tcgtcggtga tatacccgtt gtacc
//
LOCUS       DAOPOA010000574         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_75126_length_1724_cov_4.402636, whole genome shotgun sequence.
ACCESSION   DAOPOA010000574 DAOPOA010000000
VERSION     DAOPOA010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75126_length_1724_cov_4.402636"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1724)
                     /locus_tag="PKJ17_10580"
     CDS             complement(<1..>1724)
                     /locus_tag="PKJ17_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOD76465.1"
                     /translation="IELSAASQKGLLVSLIQRFFTDQLEFINIAKRFLRIDDFFPLLE
                     TVIYPPASHGKLGGKSAGLFLAMAILRAAGERFPELSSVKSPRTWYIVSDGLVDFIRL
                     NSLEEVHNQKYVEIDQARREYPHIVQIFKNSRFSSEMQDKLSVLLDDLEGKPIIVRSS
                     SLLEDRLGAAFSGKYKSLFLANEGTKRQRLAALTDAIAEVYASIFGPDPIEYRAERGL
                     LDMHEEMGIMIQEVVGQRVGPYFLPAYAGVGFSHNEFRWSHRIKREDGLLRVVPGLGT
                     RAVDRVKDDYPILLAPGQPGLRVNISAEEVLRYSPKKIDVINLETGAFETIDAHELLR
                     DFGREYPAVHHLVSIVKDHGLAIPPAFGPDFERDEVVITCDGLVRKTPFMQTMRQMIS
                     VLEEALETPVDIEFASDGTDLYLLQCRPQSHAVDSTPTVIPKNIPPERVIFTAERYVS
                     NGRVPVITHIVYVDPDRYNEISELDDLKAVGKAVGRLNNLLPKRQFILMGPGRWGSRG
                     DIKLGVNVTYSDINNTAMLIEVARKKGSYVPDLSFGTHFFQDLVESGIRYLPLYPGDE
                     GIIFNEVF"
BASE COUNT          328 a          508 c          551 g          337 t
ORIGIN      
        1 gaagacctcg ttgaagatga tcccctcgtc tccggggtag agcgggaggt agcggatgcc
       61 ggattccacg aggtcctgga agaagtgcgt cccgaaagag aggtcgggaa cgtagctccc
      121 cttcttccgc gcgacctcga tcaacatggc cgtgttgttg atgtccgaat aggtgacgtt
      181 gacgcccagc ttgatatcgc cccggctccc ccaccggccg ggacccatca ggatgaactg
      241 ccgtttcggt aggaggttgt tgaggcggcc gaccgccttg cccaccgctt tgaggtcgtc
      301 gagctcggag atctcgttgt accgatccgg atcgacatag acgatatgcg tgatgaccgg
      361 cacccggccg ttgctcacat accgctcggc cgtgaagatc acccgttccg gcggaatgtt
      421 cttggggatc acggtcgggg tgctgtcgac ggcgtggctt tggggccggc attggaggag
      481 atacaggtct gtcccgtcgg acgcgaactc gatgtcgacc ggggtctcga gagcttcctc
      541 gagcacggag atcatctggc gcatggtctg catgaacggc gttttccgga cgaggccgtc
      601 gcaggtgatg acgacctcgt ccctctcgaa gtcgggcccg aacgctggcg ggatcgcgag
      661 accatggtct ttcacaatcg agaccaggtg atgaaccgct gggtactcgc gtccgaaatc
      721 gcgaagaagc tcgtgggcgt cgatcgtctc gaaggcgccc gtttccaggt tgatcacgtc
      781 gatcttcttc ggggagtagc ggagaacctc ctcggcactg atgttgacgc gcaaccccgg
      841 ctggcccggg gcaagcagga tcggatagtc gtccttgaca cgatcgaccg cgcgcgtgcc
      901 gaggcccggg acgacacgca acagcccgtc ctcgcgtttg atccgatgcg accagcggaa
      961 ctcgttatgg ctgaatccga cgccggcgta ggcagggagg aagtagggcc cgactctctg
     1021 ccccaccacc tcctggatca tgatccccat ctcctcgtgc atgtccagga gcccgcgctc
     1081 ggcccggtac tcgatcggat ccggtccgaa gatcgaggca tacacctccg cgatagcgtc
     1141 ggtgagagcg gccagccgct gccgcttcgt cccctcattg gccaggaaga ggctcttgta
     1201 cttgcccgaa aaggcggctc cgagccggtc ctccaggagg ctggagctgc ggacaatgat
     1261 gggctttccc tccaggtcgt ccaggagcac ggaaagtttg tcctgcatct ccgatgaaaa
     1321 acgggagttc ttgaagatct gcacgatgtg agggtactca cggcgagcct ggtcgatctc
     1381 cacgtacttc tggttatgaa cttcctcgag gctgttcagg cgaatgaagt ccaccagtcc
     1441 atcggagacg atgtaccagg tcctgggaga tttgacggag gagagttcgg ggaatcgctc
     1501 accggcggcg cgcaggatcg ccatcgccag gaagagcccg gcgctctttc cgccaagttt
     1561 cccgtggctg gccggagggt agatgaccgt ctccaggagc gggaagaagt cgtcgatccg
     1621 aaggaacctc ttcgcaatgt tgatgaactc gagctgatcc gtgaagaacc gctgtatcag
     1681 cgagacgaga agacccttct gtgacgcagc cgagagttcg atct
//
LOCUS       DAOPOA010000575         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_75318_length_1721_cov_3.532413, whole genome shotgun sequence.
ACCESSION   DAOPOA010000575 DAOPOA010000000
VERSION     DAOPOA010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75318_length_1721_cov_3.532413"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1213
                     /locus_tag="PKJ17_10585"
     CDS             8..1213
                     /locus_tag="PKJ17_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOD76466.1"
                     /translation="MESNCVTCGQCILACPVGALYEKDDVNAVWKALQDPKKHVVVQE
                     APAIRAALGEEFDLPPGTLVTGKMIAALRRLGFDKVFDTNFTADLTIIEEGNELLKRV
                     KEGGTLPMITSCSPGWIKFCEHFYPELLGHLSTCKSPQQMFGALAKTYYAETAGIDPK
                     DIVVVSIMPCTAKKFEAQRPEMASSGFRDVDYVLTTRELGRMIKEAGIDFINLPDEDY
                     DAPMGEYTGAGTIFGATGGVMEAAVRSAYRLVTGRELEDVEIKPVRGLKGIKEAEIDI
                     DGTKVKVAVAHSCGQARALLDKVRDQIAKDGKSEYQFIEVMACPGGCVGGGGQSWGSN
                     IAKRARRGESLYEEDRSLPWRRSHENPSVQALYEKYLEKPNSEKAHKLLHTHYYKRST
                     VDGKVLPNK"
     gene            1252..>1721
                     /locus_tag="PKJ17_10590"
     CDS             1252..>1721
                     /locus_tag="PKJ17_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOD76467.1"
                     /translation="MSEKQCHCAEVQEEELYPRLEEVIMEHRGKGEDLIMVLHKAQNL
                     FGYLPRKAQEMVAEGLNVSLNEVYGVITFYNFFSTVPQGRNSVKVCLGTACYVRGSQE
                     VLDKAQRELGIKVGGTTEDRRYSLDVVRCIGACGLAPAMLVNEDVYGRIKPPHL"
BASE COUNT          419 a          448 c          521 g          333 t
ORIGIN      
        1 tccgcttttg gaatctaatt gcgtaacctg cggtcagtgc attctggcct gcccggtggg
       61 agcgctatat gaaaaggacg acgtcaacgc cgtctggaaa gctttacagg atcctaaaaa
      121 acatgttgtg gttcaggaag caccggcgat ccgcgccgcc ttgggcgaag aatttgatct
      181 gcccccgggc acgctggtca cgggaaaaat gatcgctgct ttaaggagac ttggatttga
      241 taaggttttt gataccaact tcacggcgga cctgaccatc attgaggaag gcaacgaatt
      301 gctcaaaaga gtcaaggaag gcggcacatt gccgatgatt acatcttgca gcccgggctg
      361 gattaaattc tgcgaacatt tttatcccga gctgctgggc catctctcca cctgcaagtc
      421 cccacagcag atgttcggtg ctttagccaa aacctattat gccgaaacgg caggtatcga
      481 tcccaaagat atcgtcgtcg tttcgatcat gccctgcacg gcgaaaaaat tcgaagcgca
      541 gcgtccggaa atggccagca gcggtttccg cgatgtcgat tacgtattga cgaccaggga
      601 actgggccgc atgattaagg aagccggcat cgatttcatc aatctgcccg acgaagacta
      661 cgacgctccg atgggcgaat acaccggagc cggaaccatc ttcggcgcca cgggcggtgt
      721 tatggaagcc gcggtgcgga gtgcctaccg tctcgtcacg ggacgggaac tggaggacgt
      781 ggagataaag cccgtcaggg gtcttaaggg gataaaagag gccgagatcg acatcgatgg
      841 gacgaaggtg aaggtcgccg tggcccacag ttgcggccag gcacgggcgc ttctcgacaa
      901 ggtaagggac cagatcgcga aggatggaaa gtccgagtac cagttcatcg aggtcatggc
      961 ctgtcccggc ggatgcgtcg gcggcggagg ccagtcctgg ggcagcaata tcgcgaagcg
     1021 ggcacgcagg ggtgagtccc tgtacgagga agaccgcagc ctgccctgga ggcgttccca
     1081 cgagaacccc tccgtacagg cgctgtacga gaaatatctg gagaaaccaa attcggagaa
     1141 ggcccacaaa cttctccata ctcattacta taaacggagt accgtggacg gcaaggtttt
     1201 gccgaacaag taagttcgat cgccaggtta ctctaagagg gagggagcaa gatgtcagaa
     1261 aagcaatgcc actgtgccga ggttcaggag gaagagctct atccccggct cgaggaagtc
     1321 atcatggagc atcgggggaa gggagaagac cttatcatgg tcctgcacaa ggcgcagaac
     1381 ctgttcggtt atcttccgag aaaggcccag gagatggtcg ccgaaggcct caacgtttcg
     1441 ctcaacgagg tgtacggcgt catcaccttc tataatttct tctctaccgt accccaggga
     1501 cgcaacagcg tgaaggtctg tctcggcacg gcatgttacg tgagaggttc ccaggaggtc
     1561 ctggacaagg cccagagaga actcgggatc aaggtcggcg gcacaacgga ggacaggcgg
     1621 tattccctcg acgttgtgcg ttgcatcggt gcatgcggcc tcgcgccggc aatgctggtc
     1681 aacgaggacg tctacgggcg gatcaagcca ccgcatctca t
//
LOCUS       DAOPOA010000576         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_75873_length_1712_cov_4.042849, whole genome shotgun sequence.
ACCESSION   DAOPOA010000576 DAOPOA010000000
VERSION     DAOPOA010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75873_length_1712_cov_4.042849"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PKJ17_10595"
     CDS             complement(<1..607)
                     /locus_tag="PKJ17_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOD76468.1"
                     /translation="MTERDHIVAFARKTTGVDASTPATVAALDARGSDRAFYRFSWGE
                     GRSVIVIDYDPGRTENRYYADIARYLRSMRVPVPEVTGHDPEAHLMVLEDLGSKDLHA
                     FAGEDWPTRRALYQKTLAATKRLHGIQEKDFPSGTVKLMDGFGPELYLFEQNYFLEHF
                     VRSHCSVDLEPAFESRLMSELSRLTDELCSLRRCLVHRDLQS"
     gene            complement(594..1370)
                     /locus_tag="PKJ17_10600"
     CDS             complement(594..1370)
                     /locus_tag="PKJ17_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOD76469.1"
                     /translation="MKGPGIPTPNPMAKKRFRTAFILGAGLGTRLRPLTEHVPKPLLP
                     LNGRPMITYAMDHLIGAGIERFIVNTHHRPGVYSEIFPDARWRGIPIIFRHEPTLLDT
                     AGGLKNIGDLLTGDEAILCYNGDILSDMPLAGLMAAHEKDRPDATLLLRSEGPLRNVD
                     IDSSGAICDMRHVLGRPGAEQCLFTGIYAVETALLAHMEPGRIESVVDVFLRRIAARP
                     GSIRGIVVDEGAWHDIGSVEVYRKMDAQMKESKGIENDGT"
     gene            complement(1379..>1712)
                     /locus_tag="PKJ17_10605"
     CDS             complement(1379..>1712)
                     /locus_tag="PKJ17_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76470.1"
                     /translation="NLIDLMPLERGPSGRIFRLRITGTKGSVIIGKELEIRRWLSTSH
                     LYSSAFVVSTVGPPGGLPERFILNGAGWGHGVGLCQIGAAVMATKGFTAPQILEHYFR
                     GTRIERMY"
BASE COUNT          312 a          509 c          528 g          363 t
ORIGIN      
        1 gggactggag gtcgcggtga acgaggcagc gccgcaggga gcagagctcg tctgtcaggc
       61 gggagagttc cgacatgagt cgtgactcga aggcgggttc gaggtctacg ctgcaatggc
      121 tcctgacgaa atgctcgagg aagtagttct gctcgaagag gtatagctcc ggaccgaaac
      181 cgtccataag cttcacggtt cctgaaggaa agtccttttc ctggatgcca tggagcctct
      241 ttgtcgccgc gagggtcttc tgataaaggg cgcgccgcgt cggccaatcc tctccggcga
      301 aggcgtggag gtctttgctt cccaggtctt ccagcaccat gaggtgggcc tcagggtcat
      361 ggcccgtcac ctcggggacg ggaacgcgca tgcttcgcag gtaacgcgct atatcggcat
      421 agtagcggtt ttccgtcctc ccggggtcgt agtcgatgac gatgaccgac ctgccttccc
      481 cccaggagaa acggtagaag gcccggtccg agccgcgcgc gtcaagggct gccactgtgg
      541 cgggggtcga ggcgtcgacg ccggtcgttt tgcgggcaaa ggccactata tggtcacgtt
      601 ccgtcattct ctatcccctt tgattccttc atctgagcat ccattttccg gtagacctcg
      661 acggagccga tgtcgtgcca cgcgccttca tcgacaacga ttccccttat agagcccggc
      721 cgcgcggcga tcctccgcag aaagacgtcg accaccgatt ctatccttcc cggttccatg
      781 tgtgcgagca gtgctgtctc cacggcgtag ataccggtaa aaaggcattg ttccgcgccc
      841 ggcctcccca gcacgtgccg catgtcgcat atcgctcccg agctgtcgat atcgacattg
      901 cgcagaggcc cctcactcct caggagaagg gtggcgtcgg gacggtcctt ctcgtgcgcc
      961 gccatgagtc cggcgagagg catgtcggac aggatatcgc cgttatagca cagtatggcc
     1021 tcgtcaccgg tcagaaggtc cccgatgttc ttgagcccgc cggcggtatc gaggagcgtc
     1081 ggttcgtgcc tgaagataat ggggataccc cgccagcggg cgtcgggaaa gatctcactg
     1141 tagacccccg gccggtgatg ggtgttgacg atgaaccgct ctatgcctgc cccgatgaga
     1201 tggtccatgg catatgtgat catggggcgg ccgttcagcg gaagaagagg tttgggaaca
     1261 tgctctgtca gaggcctgag ccgcgttccc agacccgcgc caaggatgaa ggctgtcctg
     1321 aaccgtttct ttgccatggg gtttggggtg gggatgccgg gacctttcat ggagactctc
     1381 agtacatcct ctctatgcgc gtgcccctga aatagtgttc cagtatctgc ggtgccgtga
     1441 accccttcgt cgccatgacc gcggcgccta tctggcacag gccgacgcca tggccccatc
     1501 cggccccgtt gagaatgaac ctttcgggca ggccgcccgg cggacccacc gtggaaacga
     1561 caaaggcgct gctgtaaaga tggcttgtcg agagccatct gcgtatctcc agttccttgc
     1621 ctatgatgac gcttcccttc gttcccgtta tcctgaggcg gaaaattcgc cccgatggtc
     1681 ctctttcaag gggcatgaga tcgatgagat tg
//
LOCUS       DAOPOA010000577         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_75878_length_1712_cov_3.365118, whole genome shotgun sequence.
ACCESSION   DAOPOA010000577 DAOPOA010000000
VERSION     DAOPOA010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75878_length_1712_cov_3.365118"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKJ17_10610"
     CDS             complement(<1..199)
                     /locus_tag="PKJ17_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76471.1"
                     /translation="MYRLGIDIGSVSVNVAVVNENGKVVRSQYIRHKGKPFVTAKEAI
                     EEAAGAYDVEFIATTGTGAKVF"
     gene            348..623
                     /locus_tag="PKJ17_10615"
     CDS             348..623
                     /locus_tag="PKJ17_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOD76472.1"
                     /translation="MQARAHIVVHGTVQGVFFRYSTQREAQGLGLTGWVRNLPDGSVE
                     IVCEGEKDAVQALVDWSRRGPRGAFVETADVSWEDHTGRFLTFEIRY"
     gene            607..1251
                     /locus_tag="PKJ17_10620"
     CDS             607..1251
                     /locus_tag="PKJ17_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOD76473.1"
                     /translation="MRSDTEKHHYYPLFLDVTGKECLVVGGGSVAERKALMLLKFNAS
                     VTVVSPKVTKKLADLADRGVLRVRTRKYRRSDLDNAVVVFACTDDRDTNGAVREDAAK
                     KGVLVNVVDKPKECDFIVPSIIRKGDVTIAISTSGTLPMASKKIRQAIERTVTKDYVS
                     YTRIVGAFRRHLIDTVPDPLRRKAIMKKISAMEVGDVVRRGLTGMKKVLDEGLS"
     gene            1248..>1712
                     /gene="ccsA"
                     /locus_tag="PKJ17_10625"
     CDS             1248..>1712
                     /gene="ccsA"
                     /locus_tag="PKJ17_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOD76474.1"
                     /translation="MNVYLYNTALGLYLASTFIYAVSLASNRPRFEKPGYYLLAAAFV
                     VHFASTAARFFEAGYTPITNIYESLSFLALCIAGFFMYIRKTYRVEMVGSIIAPLVSL
                     VVIMSLGFPSEIRPLIPALRSAWLPVHTVLSFLGNGIFVVGFFISILYLLIEK"
BASE COUNT          358 a          519 c          473 g          362 t
ORIGIN      
        1 cgaagacctt tgccccggtg cctgtggtcg cgatgaactc cacgtcgtag gccccggcgg
       61 cctcctcgat cgcctctttc gccgtgacga aaggtttccc cttgtgacgg atatactgag
      121 acctgacgac cttcccgttc tcgttgacaa ccgcgacgtt gacgctaacc gaaccgatat
      181 ctatacccag tctgtacata atcgccttct cccaagtggt caacacaact ggattttgcg
      241 ggaatgttgt gataagtata atacaaaaaa cctcttgcca acaataaaat gtttacggac
      301 gggcgttggc ggggtttttc ggcatccaaa gaggtcgttg tttcaccatg caggcacggg
      361 cacatatagt tgtacacggc acagtgcagg gcgttttctt tcgttacagc acccaacgtg
      421 aggcccaggg cctcgggctg acgggctggg tcaggaacct ccccgacggc agcgtggaga
      481 tcgtctgtga aggcgagaaa gacgccgtcc aggcgctggt cgactggtcc cgcagaggtc
      541 cccggggcgc cttcgtcgag acggccgatg tctcctggga agatcatacc ggcaggttcc
      601 ttacttttga gatcagatac tgaaaagcat cattattatc ccctcttcct cgacgtgacg
      661 ggaaaggaat gccttgtcgt cggcggcggg agtgtcgccg agcgcaaggc cctcatgctg
      721 ctcaagttca acgcctcggt gaccgtcgtg agcccgaagg tgacgaagaa gctggcagac
      781 ctcgccgaca ggggggtgct gcgcgttcgc acgcggaaat accgccggag cgacctcgac
      841 aacgcggtgg tggtgttcgc ctgcaccgac gacagggaca cgaacggcgc cgtccgcgag
      901 gacgcggcaa agaagggggt cctcgtcaat gtcgtcgaca aaccgaaaga gtgcgatttc
      961 attgtcccct ccatcatcag gaaaggggac gtgacgatag cgatatcaac gtcggggacc
     1021 ctccccatgg catccaagaa gatacggcag gccatcgaaa gaacggtgac gaaggactac
     1081 gtctcataca cgcgcatcgt aggcgccttc agaaggcatc tcatcgacac cgtccccgac
     1141 cccttgaggc gcaaggccat catgaagaag atatccgcca tggaggtggg cgacgtcgtc
     1201 aggcgcggcc tcacaggcat gaaaaaggtc ctcgacgaag ggctctcatg aacgtttacc
     1261 tctacaacac ggcactcggg ctctatctcg cgtccacctt catctatgcc gtgagcctcg
     1321 cctcgaacag gccgcgcttc gagaaaccgg gatactatct tcttgccgcc gccttcgtgg
     1381 tccacttcgc ctccacggcg gcgcgattct tcgaggccgg ctataccccc atcacgaaca
     1441 tctacgaatc cctttccttc ctggcactgt gcatcgccgg tttcttcatg tacatacgga
     1501 agacctatcg cgtggagatg gtggggagta tcatcgcgcc tcttgtctct cttgtcgtca
     1561 tcatgtccct cggcttcccg agcgagatac ggcccctcat cccggcgttg aggagcgcct
     1621 ggcttcccgt ccataccgtc ctttcattcc tgggaaacgg gatattcgtc gtcggcttct
     1681 tcatatccat cctctatctc ctcatagaga ag
//
LOCUS       DAOPOA010000578         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_76119_length_1708_cov_3.476709, whole genome shotgun sequence.
ACCESSION   DAOPOA010000578 DAOPOA010000000
VERSION     DAOPOA010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76119_length_1708_cov_3.476709"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1708
                     /gene="dnaE"
                     /locus_tag="PKJ17_10630"
     CDS             <1..>1708
                     /gene="dnaE"
                     /locus_tag="PKJ17_10630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOD76475.1"
                     /translation="IFSKEAREAGIRPIIGAELTLEGDHHLTLLIENARGYANLSQLI
                     TRAHLAGSKGEPVLSEAELPGRTEGLICLSGCAKGKIPRLLMKGEKHAACDAAKGYRA
                     LFGRHSFYIEVEGHLLPEDRRLSLQLTDLAEALGLKTVASNNVHYATRAEHILHDVLA
                     CIKHRAPLDRSAPFRRLNSEFYLKGYEEMTELPWLPREAIERTGEVAGRCAFDLDFSS
                     SSYPLFPLPEGETATGFLRRLAVENARIRYGFLTEELLARIDDEIVLIEKKGLSGYFL
                     VVRDIVEHARKMGISAQGRGSAASSVVAYALGITPVDPMRHKLFVGRFLNELSIPDID
                     IDIATNRREEVIQYVYDTYGRDHAAMVCTYVTFQGRGAIREVGKVLGLPAHILDRMAK
                     TISAYGGRAAIDSLKEVAEFASYLDSGAWEHFGDLCTKIADFPRHLSIHVGGMVLTPR
                     PIYEIVPQEHARAEGRIVCQWDKDSVDDAGLIKFDLLGLRMLSLIDDAKELIREQKGL
                     DLNLDSISPDDPEVYRMIGDADTVGVFQVESRAQMQTLPKVRPRSFEDLTMEVAIVRP
                     GPL"
BASE COUNT          369 a          538 c          488 g          313 t
ORIGIN      
        1 gcatcttttc gaaggaggcc agggaggcgg gaatacggcc catcatcgga gcggaactca
       61 ccctggaagg ggaccaccac ctgacacttc tcatagaaaa cgccaggggc tacgccaacc
      121 tttcgcagct catcacccgg gcacatctcg ccgggtccaa gggagagccc gtgctctccg
      181 aggcggaact gcccggccgc acggaaggtc ttatctgcct gtctgggtgc gcaaaaggaa
      241 agatcccccg ccttctcatg aagggcgaaa aacacgcggc ctgcgatgcg gcaaagggat
      301 atagggccct tttcggcaga cacagctttt acatagaggt cgaaggccac ctgctccccg
      361 aagacaggcg cctgtccctg cagcttacag atctggcaga ggccctcggc cttaagaccg
      421 tcgcatcgaa caacgtccac tatgcgacac gggccgagca tatccttcac gacgttcttg
      481 cctgcataaa acaccgggca cccctcgaca ggtccgcccc tttccggagg ttgaactccg
      541 aattctatct caagggatac gaagagatga ctgaactccc ctggctcccc cgggaggcaa
      601 tagaaaggac cggagaggtg gccggccgtt gcgccttcga tcttgatttt tcctcttctt
      661 cctatcccct ctttcccctg ccggagggag aaacggcaac ggggttcctg cggaggctcg
      721 ccgtggagaa cgcccgcata cgctacggct tcctcacgga agagctcctt gcgaggatcg
      781 atgacgagat cgtgcttatc gagaaaaagg ggctttcggg atatttcctc gtcgtccggg
      841 acatcgtcga gcatgcgaga aagatgggga tatcggcgca gggaagaggt tcggcggcaa
      901 gctccgtcgt cgcctacgcc ctcgggatca cccccgtcga tcccatgagg cacaagcttt
      961 tcgtggggcg cttcctcaac gaactcagca tccccgacat cgacatcgat atcgcgacga
     1021 acaggagaga ggaggtcata cagtacgtct acgatacata tggtcgcgac catgccgcca
     1081 tggtctgcac ctacgtgacc tttcagggac ggggcgctat acgggaggtt ggaaaggtcc
     1141 tgggactccc cgcgcacatc ctcgacagaa tggcaaagac catctcggcc tatggaggcc
     1201 gcgccgccat cgattccctg aaggaggtcg ccgaattcgc gtcctacctc gattccggcg
     1261 cctgggagca tttcggagac ctctgcacga aaatagccga ctttccacgg catctgtcca
     1321 ttcacgtggg cggcatggtc ctcaccccca ggcccatata tgagatcgtc ccccaggagc
     1381 acgcccgcgc ggaagggcgg atcgtctgcc agtgggacaa ggactccgtc gatgacgcgg
     1441 gcctcatcaa gttcgacctt ctcggcctga ggatgctctc ccttatcgat gacgcgaagg
     1501 aactcatacg cgaacagaag ggcctcgacc tcaacctcga cagcatttcc ccggacgatc
     1561 ccgaggtcta tagaatgatc ggcgatgccg atacggtcgg cgtcttccag gtggaaagcc
     1621 gggcccagat gcaaaccctt cccaaggtga gaccccggtc cttcgaggac ctcaccatgg
     1681 aagtggccat cgtgagaccc gggcccct
//
LOCUS       DAOPOA010000579         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_76663_length_1700_cov_2.522796, whole genome shotgun sequence.
ACCESSION   DAOPOA010000579 DAOPOA010000000
VERSION     DAOPOA010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76663_length_1700_cov_2.522796"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..607)
                     /locus_tag="PKJ17_10635"
     CDS             complement(140..607)
                     /locus_tag="PKJ17_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76476.1"
                     /translation="MMASVRTGVCLGLISLMLLAATATAACLVVSAGDGGGPLVSLPV
                     EGGCIVHLDFVNSIYLAPVRETFLHETGKGLSVIRVESPSAGVFEYYRLESDGPSAEL
                     YRNVGSIHLKSHDYRDHTITVGTTVLHLKDFAAGGDPLTISVHDGVCGSGQGR"
     gene            complement(642..>1700)
                     /locus_tag="PKJ17_10640"
     CDS             complement(642..>1700)
                     /locus_tag="PKJ17_10640"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOD76477.1"
                     /translation="RLTSPEGRQYHIAAAFPSASGKTNLAMIQPTVDGWKAECIGDDI
                     AWMKIRPDGRLHAINPEAGFFGVAPGTSYASNPAAMDTIKENCIFTNCGLTDDGDIWW
                     EGMTEEPPTHTIDWRGQDWTPGSKDPTAHPNARFTAPAQQCPIISPDWEKLEGVPIDI
                     FIFGGRRAATVPLVHEAFSWEHGVFMGATASSETTAANIGSIGDVRRDPFAMKPFCGY
                     HMGDYFQHWLDMGKRLGDKAPRIFYTNWFRKTADGRWLWPGFGENSRVLKWMCERVEG
                     KAGAQETAIGYMPSVEDLDLTGLDVAAEDMKELLRIDVDAWKEEVRDMEKHFAQFGDR
                     LPQVLAGELKALAKRLGS"
BASE COUNT          311 a          539 c          510 g          340 t
ORIGIN      
        1 ccaagggcct ctgcggcgcc ggaagcgtta aagccctcat agtccgggac catgggcgcg
       61 catgatacga gaagcacgaa agttatggcg gcggtgacaa cccccgtaaa cccggtgcgg
      121 gacctccgcg cggaacccat tatcgcccct gacccgaccc gcagacccca tcatgcacgg
      181 agatggtcaa cgggtcgcct cccgcggcaa agtccttcag atgaagaacg gttgtaccca
      241 cggtgatggt gtggtccctg taatcgtggc ttttcaggtg aatggagccc acgttgcggt
      301 acagttccgc cgagggcccg tcgctctcaa gcctgtagta ctcgaagaca ccggcggagg
      361 ggctttccac ccgtatgacc gaaagcccct tgccggtctc atgaaggaag gtctcccgca
      421 cgggagcaag gtagatcgaa ttgacgaaat caaggtgaac gatgcatcca ccctcgacgg
      481 gaagtgaaac aaggggtcct cccccgtctc ccgccgatac cacgaggcat gccgccgtcg
      541 cggttgccgc tagcagcatc agggatatca gacccaggca cacgccggtg cggacggacg
      601 ccatcatcaa cctcctgaaa ggacgcgatg gtcccggccg tctagcttcc cagcctcttc
      661 gcgagcgcct tcaattcccc tgccagaacc tggggcaggc ggtctccaaa ctgggcgaaa
      721 tgcttctcca tgtccctgac ctcctctttc caggcatcga catctatgcg cagcagctct
      781 ttcatatcct cggcggccac atcaagtccg gtgaggtcga ggtcttccac cgacggcatg
      841 tagcctatgg ccgtttcctg tgctcccgcc ttgccttcga ctctctcgca catccatttc
      901 aggacgcggc tgttctcccc gaaaccgggc cagagccacc tgccgtcggc ggtctttctg
      961 aaccagttgg tgtaaaagat gcggggtgcc ttgtctccca ggcgcttgcc catgtcgagc
     1021 cagtgctgga agtaatctcc catgtggtag ccgcagaagg gcttcatcgc aaaggggtcg
     1081 cggcgcacgt cgccgatgga gccgatgttc gcggccgttg tctccgacga ggcggtcgcc
     1141 cccatgaaaa caccgtgttc ccagctgaat gcctcatgga cgagcggtac cgtggcggca
     1201 cggcggcctc cgaagatgaa gatgtcaatg gggacgcctt caagcttctc ccagtcggga
     1261 ctgatgatgg ggcattgttg cgccggggcc gtgaagcggg cgttgggatg cgccgtcggg
     1321 tccttcgatc cgggtgtcca gtcctgtccg cgccagtcga tggtgtgggt tggcggctct
     1381 tccgtcatcc cctcccacca gatatcgcca tcgtctgtga ggccgcagtt cgtgaagatg
     1441 cagttctcct tgatcgtgtc catggcggcc gggttggacg cataggacgt gccgggcgcc
     1501 actccaaaga aacccgcctc ggggttgata gcatggagcc tcccatcggg cctgatcttc
     1561 atccaggcta tgtcgtcacc gatacactcg gccttccagc cgtctaccgt gggctgtatc
     1621 atggcaaggt tggtcttgcc cgaggctgaa gggaacgcgg ctgctatgtg gtactgcctt
     1681 ccctcgggac ttgtgagacg
//
LOCUS       DAOPOA010000580         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_76926_length_1696_cov_2.298598, whole genome shotgun sequence.
ACCESSION   DAOPOA010000580 DAOPOA010000000
VERSION     DAOPOA010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76926_length_1696_cov_2.298598"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..311
                     /locus_tag="PKJ17_10645"
     CDS             30..311
                     /locus_tag="PKJ17_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76478.1"
                     /translation="MPPPSRWWTGQNFIDMAPYINDARAQQLMLLGGVTGQDVPGYHD
                     WNNILGRLGLLNLDHTLANTSHVFGSLIMTAAFIWAAIILFMQWKNRDR"
     gene            complement(484..1041)
                     /locus_tag="PKJ17_10650"
     CDS             complement(484..1041)
                     /locus_tag="PKJ17_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HOD76479.1"
                     /translation="MEEKLANPAPLGLMGFGMTTVLLNIHNAGFFPVGSMILAMGAFY
                     GGLAQIIAGILEYKKGNTFGVTAFTSYGLFWWTLVFILVFKGLPGNPESFMGWYLFMW
                     GLFTFFMWFGTWGKNRGIQFVFLSLFILFWLLAIRDWTGSTAIGTLAGWEGIICGLSA
                     VYLAMAEVLNEVHGRTVLPIGEIKK"
     gene            complement(1225..>1696)
                     /locus_tag="PKJ17_10655"
     CDS             complement(1225..>1696)
                     /locus_tag="PKJ17_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOD76480.1"
                     /translation="VAYCIAGIAVVIARSILVFSTIKYLGAAYLLYIGIRSVVSRQPG
                     MEADVVTKRKDISPFRAVRMGFFTNILNPKATLFFLGLFTVVISPAAPPPRHILVIAG
                     IVMVVDTMLWFGFVALFLNERHVRSFFERFQGYFNRAFGGVLILLSIKIALSRD"
BASE COUNT          399 a          496 c          459 g          342 t
ORIGIN      
        1 cgccgccttt ctgaaacggg gcgacgcctt tgccgcctcc ttcgcgctgg tggacgggac
       61 agaatttcat cgacatggcc ccctacatca acgacgcccg agctcagcag ctgatgctcc
      121 tcggcggcgt gacgggacag gacgtccccg gataccacga ctggaacaac atcctcgggc
      181 gccttggcct gctcaacctc gaccacactc tggccaacac gtcacacgtc ttcggctccc
      241 tcatcatgac agccgccttc atctgggcag ccatcatact cttcatgcag tggaagaaca
      301 gggacaggtg agaagacggt ctcaagtctc aagtttcagg tttcaggttt taggccaaaa
      361 gccaaagaaa cgcgcgtgaa gctatacata ccgtgtgaca aatacgcccc tctgccttat
      421 cggctggagg ggcgtatttc ttgcctgtgc ttgaaacgtg agacctgaga cctgaaactg
      481 tccttacttc tttatctccc ctatgggaag gacggtcctt ccgtgcacct cgttcaagac
      541 ctcggccatt gccaggtaga cggcggagag gccgcagatg atgccttccc atccggcaag
      601 ggtgccgatg gcggtactgc ccgtccagtc acggatggca agaagccaga agaggatgaa
      661 aaggctcagg aacacgaact gtatgcccct gttcttgccc cacgtaccga accacatgaa
      721 gaaggtgaac aggccccaca tgaagaggta ccatcccatg aaggattcgg ggttcccggg
      781 aagtcccttg aagacgagaa tgaatacgag ggtccaccag aaaaggccgt acgacgtgaa
      841 ggccgttacg ccgaaggtgt ttcctttctt gtactccagg atgccagcaa tgatctgggc
      901 aaggccgccg taaaaggctc ccatcgcgag tatcatcgat cccacgggga agaaccccgc
      961 gttgtggatg ttcagaagca cggtggtcat accgaatccc atgaggccga gtggtgcggg
     1021 gttagcaagc ttttcttcca tgacaaactc ctccttatga tggtcgatag ttcacaccac
     1081 cccttcagga caaaccctat ctttccctct ttgccttatg cgttttgaag acccttgagc
     1141 ttccggacgg aaacccgtat tcactatagc acgtcgaggg gtgcctgtca tcacttttgc
     1201 ttcgtgcgtg tctccacggg acggctaatc gcgcgaaagc gctatcttaa tgctcagaag
     1261 gatgagcact cccccgaagg ccctgttgaa ataaccctga aacctctcaa aaaaggacct
     1321 cacgtgcctc tcgttgagga aaagggccac gaaaccgaac cacagcatgg tgtccaccac
     1381 catgacaatg cccgcgatga cgagaatatg gcggggcggg ggcgcggcag gagatatgac
     1441 gaccgtgaaa aggccgagga aaaagagcgt cgccttgggg ttcaggatgt tcgtgaaaaa
     1501 ccccatgcgg actgccctga agggagagat gtctttcctt ttcgtcacca catccgcctc
     1561 catcccgggc tgccggctga cgaccgaccg tatccctatg tacagcagat atgccgcccc
     1621 caggtacttg atcgtgctga agacgaggat ggatcgcgcg atgacgacgg ctatcccggc
     1681 tatgcagtag gcaacg
//
LOCUS       DAOPOA010000581         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_77094_length_1693_cov_2.458486, whole genome shotgun sequence.
ACCESSION   DAOPOA010000581 DAOPOA010000000
VERSION     DAOPOA010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77094_length_1693_cov_2.458486"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..537
                     /locus_tag="PKJ17_10660"
     CDS             148..537
                     /locus_tag="PKJ17_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD76481.1"
                     /translation="MAKKKVLLIDDNRDFCEVTKLWLHYTWKYRVVTAANGPMGIDLA
                     RKVRPDVILLDVRMPVMDGGRVAEHLMEDNSTSDIPIIFLTGLVRHEEVEKSGGFIAG
                     RPFIAKPFHLEELTGMIETVTAGPRAA"
     gene            complement(543..893)
                     /locus_tag="PKJ17_10665"
     CDS             complement(543..893)
                     /locus_tag="PKJ17_10665"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOD76482.1"
                     /translation="MKITDLSKVEKVPMTMEGAKGAWKQIPLGQADGVPAYTLRVFTI
                     EPGGHTPYHHHPFEHMNYIIEGEGTLVDAEGRQRPVRKGDFALVLPDEVHQYRNTATN
                     RPLVMICLVPKDYE"
     gene            complement(1469..>1693)
                     /locus_tag="PKJ17_10670"
     CDS             complement(1469..>1693)
                     /locus_tag="PKJ17_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76483.1"
                     /translation="ASRRSSRGGPRRFATIFSSAGIRSITIAIMEVPCCSKMPLIVEA
                     ALKRSGKKIPVETIVINPRGEIVRRTKLAA"
BASE COUNT          348 a          461 c          509 g          375 t
ORIGIN      
        1 gagacttgcg acctgcgaca ttctttttga cctgcgactt gagacttgcg acctgtgaca
       61 tttctctgac ctgcgacatc tcttaggccc tttgcggtct taaggaacac gccgatgtgc
      121 tgataatagt atcaaagggg atcgaagatg gcgaagaaga aggtccttct tatcgatgac
      181 aaccgggatt tttgcgaggt cacaaaactc tggcttcact atacctggaa gtacagggtg
      241 gtgacggcgg caaacgggcc catggggatc gacctcgcca ggaaggtgag gcccgacgtc
      301 atcctactcg acgtcaggat gcccgtaatg gatggcggca gggttgccga gcatctcatg
      361 gaagacaact ccaccagtga tatacccatc atattcctta caggcctcgt caggcacgaa
      421 gaggtggaaa aaagcggtgg tttcattgcg ggccgtccct tcatcgccaa gccctttcac
      481 ctcgaagagc tcacgggcat gatcgagacc gtgacggccg gcccaagggc tgcctgacac
      541 agctattcgt aatccttcgg cacgaggcag atcattacca ggggtcggtt cgtggcggta
      601 ttcctgtact ggtggacctc gtcaggcaga acgagtgcga aatctccctt tcggaccggc
      661 cgctgtctgc cttccgcgtc gacgagagtc ccttcaccct ctatgatgta gttcatatgc
      721 tcgaaagggt ggtggtggta cggggtgtgc cccccgggct cgatggtgaa gacccgaagc
      781 gtgtaggcgg gaaccccgtc tgcctgtccg agagggatct gcttccatgc tccctttgcc
      841 ccctccatgg tcatggggac cttctccact ttactgagat cagtgatctt catagcctcg
      901 ctccttgtgg ggaatattat gcagaatcag ccattatacc acagcacagc acccggtcaa
      961 aattgcgctg ctcagacctt gggccaacag ggggcagcga agctcaatgc ccaatctcac
     1021 gcccgcttcg ctagagggca cagaggtgat gaggagagcg cgcagagaga agatccggcc
     1081 tgatcccggc gtgcccgccg gaatcaggcc gtctttgcat cccgtctccg acgggagaaa
     1141 aagaagagga ttcttctcag gtgggaggga tgggagttgg ggggtaaggg aggatggggt
     1201 ataggtccta tgactacagg ggggatgagg gggataactt tttcaggata aggaaaaaga
     1261 ctatttgttt ctctcccgcc gggacggcgg gacgagaagg ccgccggatt ttggtaggct
     1321 ccgaaatccg gcggcaatct tttctctcct ttcccctctc tttctaatct ctgcggactc
     1381 gagcgaagcg ggcgtgagat aatgtctttg agctttcccc ccgctcttac ctcacgacca
     1441 gggcgacgaa cgggcgtgag atgggccttt acgccgccag tttggttctc cgcacgatct
     1501 ctccccgtgg gtttatcacg atggtttcga cgggtatttt cttcccggag cgtttgagcg
     1561 ccgcctcgac gatgagaggc atcttcgagc agcaggggac ttccatgatg gcgatggtga
     1621 tgctccgtat gccggcggat gagaagatgg tcgcgaacct tctcggcccg ccccgcgagg
     1681 accggcgcga ggc
//
LOCUS       DAOPOA010000582         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_77095_length_1693_cov_2.346154, whole genome shotgun sequence.
ACCESSION   DAOPOA010000582 DAOPOA010000000
VERSION     DAOPOA010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77095_length_1693_cov_2.346154"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="PKJ17_10675"
     CDS             <1..1641
                     /locus_tag="PKJ17_10675"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD76484.1"
                     /translation="GVSPARYDEQVFCSDKVRYVGDEVAAVAAIDLETAMEAVSLIKV
                     DYEELPVLLDIGSAMAEGAIAIHEDFPGNISAEVHQEFGNFEEALKECHIVRTDTFTN
                     KRQDGGFLEPQACLARYDLNGNLTLWSSTQSVHYVQRSVAMVLGIPVGKVRVVKPYVG
                     GGFGPKAAVNAMELAACLMSRRTGKPVKMVFSREQVFLHSRARHQFTHELTTGVKKDG
                     TIFALRNVCHLDGGAYSSFGIATIYYAGSLLGAPYKLPNMKYDGYRIYTNKPTCGAQR
                     GHGGVAARAGWEQQLDIIAAELGMDPVEFRLKNMMQTGDVTCNDFNMSSLGMKECLEG
                     VRDGSNWGAKKGKLPKGKGIGVACGFFVSGAGYPIYRSETFHSTATIRLTEDGGGVNL
                     YTASAEIGQGSDTILGQIAAEALGVSLDDVRVHSGDTDFGVDLGAYSSRQTLMSGHAV
                     KMAAEDTKRKVLEALSEQLNVPVEEMDIARGVIVFKKDKPDFSRLRTLYIKEHRGWAD
                     QPSGAELTFREAARVAFITGGVIIGNGAYKPGELGGKY"
BASE COUNT          333 a          483 c          557 g          320 t
ORIGIN      
        1 ggtgtcagtc cggcgcggta cgacgagcag gtcttctgct ccgacaaggt tcgttatgtc
       61 ggcgacgagg tggctgcggt cgcggccatc gacctcgaaa cggcgatgga ggcggtgtcc
      121 cttatcaagg tcgattacga ggagcttcct gtgcttctcg atatcggatc ggccatggcg
      181 gaaggggcga tcgccataca tgaggatttt cccggaaaca tcagtgccga ggtccaccag
      241 gagttcggca acttcgagga ggccctgaag gagtgccaca tcgtcaggac cgacacgttc
      301 acgaacaaaa ggcaggacgg cggctttctg gaaccccagg cctgtcttgc ccggtacgac
      361 ctgaacggga acctcacgct gtggtcgtcg acccagtcgg tccactatgt ccagaggagc
      421 gttgccatgg tgctcggcat ccccgtcggc aaggtccgtg tcgtgaagcc ctacgtgggg
      481 ggcggttttg gccccaaagc ggcggtgaac gccatggagc tcgcggcgtg cctcatgtcg
      541 agacgcacgg gcaaaccggt gaagatggtc ttctcccggg aacaggtctt cctccattcc
      601 cgggcacggc accagttcac ccacgaattg acgacgggcg taaagaagga cgggaccatc
      661 ttcgccttga ggaacgtctg tcatcttgac ggcggcgcgt attcgagctt cggcatcgcg
      721 acgatctact atgccggttc gctcctcggc gctccctaca agcttccgaa catgaaatac
      781 gacggttatc gcatctatac gaacaaaccc acctgcggtg ctcagcgcgg gcacggaggc
      841 gttgccgcga gggcgggatg ggaacagcag ctcgacatca tcgctgccga gctcggcatg
      901 gaccccgtgg agttcaggct caagaacatg atgcagaccg gcgacgttac ctgcaacgac
      961 ttcaacatgt cgagcctcgg catgaaggag tgcctcgagg gggtacgcga cggctcgaac
     1021 tggggcgcca agaaggggaa actccccaaa ggtaagggga taggtgtggc ctgcgggttc
     1081 ttcgtgtccg gggcggggta tcccatctac cggtccgaga cgttccattc cacggcaacg
     1141 atcaggctca ccgaggacgg cggcggggtc aatctttata ccgcgtctgc ggagataggg
     1201 caggggtcgg acaccatcct cgggcagatc gccgcggagg ccctcggtgt ctctctcgat
     1261 gacgtgcggg tgcactcggg ggacaccgat ttcggcgtcg acctcggtgc ctattcgtca
     1321 cggcagaccc tcatgtccgg ccatgccgtc aagatggccg cggaagacac caagcggaag
     1381 gttctcgagg cgctttcgga gcagcttaac gtgccggtcg aggagatgga cattgccagg
     1441 ggcgtcatcg ttttcaagaa ggataaaccg gatttttccc ggttgcggac gctttatatc
     1501 aaggaacacc gcggatgggc agaccagcct tcgggtgcgg agctcacctt cagggaggcg
     1561 gcgcgtgttg cttttatcac cgggggtgtt ataatcggta atggcgctta caaacccggc
     1621 gagctgggag gtaagtacta gggtgccgcg gtgggaacat cgccggcata cggatgctcc
     1681 gcccaggtcg tcg
//
LOCUS       DAOPOA010000583         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_77151_length_1692_cov_3.248015, whole genome shotgun sequence.
ACCESSION   DAOPOA010000583 DAOPOA010000000
VERSION     DAOPOA010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77151_length_1692_cov_3.248015"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..717
                     /locus_tag="PKJ17_10680"
     CDS             106..717
                     /locus_tag="PKJ17_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HOD76485.1"
                     /translation="MMILSHTFEEFAGMVRAFHGSAAPGIMIGGSMVDLALRCVPEGG
                     LYDVICETAKCLPDAVQLLTPCSIGNGWLRILDTGRFSLAFYDKVTGKGVRVFIDPAK
                     LDAWPQIKSWFLKLTPKKEQDTARLMKEIEEAGSDICSVESIQVSLDLVKKEKGGPIA
                     LCPSCGEPYPSGRGDICPACTGGILPYGSGTGEAGQVNRRECV"
     gene            799..1272
                     /locus_tag="PKJ17_10685"
     CDS             799..1272
                     /locus_tag="PKJ17_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76486.1"
                     /translation="MVKEGKRLLIDADVQAVLEKRGIREEDIQEVLDFGEETGNVYMH
                     PDTGHLLSYFTPATTTYWVEFGRDGGAYRIYRAYSHRMEILHGFNMPAKKQQTLDWTC
                     TKCDRRLELATVKLKYMEETFGVDIPACPSCQRIFVSEEDATQRMAQAEKMLEDK"
     gene            1281..>1692
                     /locus_tag="PKJ17_10690"
     CDS             1281..>1692
                     /locus_tag="PKJ17_10690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD76487.1"
                     /translation="MENDERRACLQRMTGGTLRPGGLTLTTRALELCRMPPEARVLDA
                     GCGNGVTLRHLRERCGVRCCGLDLSMEMLAAARDEDGEGSLVRARMEEIPFRNDAFQG
                     VLCECVLSHTNAEVVVREFSRIVEEGGFLILSDLY"
BASE COUNT          367 a          450 c          568 g          307 t
ORIGIN      
        1 acaggctcac cgactgcaac gcccgggagc ccatcatggg ataccccgag tggaagggac
       61 ttctgtgctc gatacggaag gcgtgatatg gtataaaagg taggtatgat gatcctctcc
      121 cacaccttcg aggagttcgc cgggatggtc agggccttcc acggcagcgc cgccccgggc
      181 atcatgatcg gggggtccat ggtggacctc gccctgcgct gtgtacctga gggcgggctc
      241 tacgacgtca tatgcgagac ggcaaagtgc ctgcccgacg ctgtgcagct cctcacgccc
      301 tgctccatcg ggaacgggtg gctgaggata ctggacacgg gccggttctc ccttgccttc
      361 tacgacaagg tgaccggtaa gggcgtccgg gtcttcatcg acccggcaaa gctcgacgcc
      421 tggccccaga taaagagctg gttcctcaag ctcaccccca agaaggagca ggacacggcc
      481 cgcctcatga aggagataga ggaggcgggg agcgacatct gctccgttga gagcatacag
      541 gtctccctcg acctcgtgaa gaaggagaag ggcggaccca tcgccctgtg cccctcctgc
      601 ggggagccct atccctcggg acggggtgac atatgccccg cctgcacggg ggggatattg
      661 ccgtacgggt cggggacagg ggaagccggc caggtgaata ggcgtgaatg cgtctgagat
      721 tgggaaacaa taaatggata ttatgtttgt ccaccgccgg tatggggtgg acaaacgcgt
      781 taatggcaag gagagcggat ggtgaaagaa gggaaaagac tgctgatcga tgccgatgtt
      841 caggccgtcc tcgagaagag ggggatcagg gaagaggaca ttcaagaggt ccttgatttc
      901 ggagaggaaa cgggaaatgt ctacatgcac ccggacacgg gacatcttct gtcctacttc
      961 acgccggcga cgacgaccta ttgggtcgag ttcggccggg acggcggggc gtacaggata
     1021 tacagggcct attcccaccg catggagata ctgcacgggt tcaacatgcc cgcgaagaaa
     1081 cagcaaacgc ttgactggac ctgtacgaag tgtgacagga gactcgagct ggccacggtg
     1141 aagctgaaat acatggagga gaccttcggt gtcgacatac ctgcgtgtcc ttcctgccag
     1201 cgcatatttg taagcgagga agacgccact cagaggatgg cccaggccga gaagatgctc
     1261 gaggacaagt agcgcctgcc atggagaatg atgaacggag ggcatgcctt cagaggatga
     1321 cgggcgggac cttgaggccc ggtggactca ccctgacgac gagggccctc gaactgtgcc
     1381 gtatgcctcc cgaggcccgg gtccttgatg cggggtgcgg caacggtgtg acgctcaggc
     1441 atctcaggga acgatgcggg gtacggtgtt gcggcctaga cctttccatg gagatgcttg
     1501 cggcagcgag ggacgaggac ggggagggtt ctctcgtgcg ggcaaggatg gaagagattc
     1561 ccttcaggaa cgacgccttt caaggcgtcc tgtgcgagtg tgttctctcc cacaccaacg
     1621 cggaggtggt ggtgagggag ttctccagga tcgtggaaga aggcggcttc ctcattcttt
     1681 ccgacctgta tc
//
LOCUS       DAOPOA010000584         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_77210_length_1691_cov_4.155868, whole genome shotgun sequence.
ACCESSION   DAOPOA010000584 DAOPOA010000000
VERSION     DAOPOA010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77210_length_1691_cov_4.155868"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PKJ17_10695"
     CDS             <1..529
                     /locus_tag="PKJ17_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOD76488.1"
                     /translation="VRDLRLGKFDVLVGVNLLREGLDLPEVSLVAILDADKEGFLRSQ
                     TSLIQTCGRAARNLNGRVIMFADNITQSMRRAIDETERRRAVQSAYNEENGITPEGIR
                     KSIVDVLSSIYEKDYYEVPVDELDAKGLKPKQLSKMVKKLKKEIAEAAKKWDFEKAAL
                     LRDRLLAIEKTELTL"
     gene            526..>1691
                     /gene="uvrC"
                     /locus_tag="PKJ17_10700"
     CDS             526..>1691
                     /gene="uvrC"
                     /locus_tag="PKJ17_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOD76489.1"
                     /translation="MTPGIRTDGLPESPGVYLFKDHDDGIIYIGKARNIRDRVRSYFR
                     DDVKDIKTRTLAETIHAVDFILTANEKEAFLLENNLIKQHVPKYNIDLKDDKSYISLQ
                     LTIKDEYPALFATRKIVDDGSLYFGPYPHARDVRDILKIIERLYPVRKCKMTVFRKRK
                     RPCMLYELGKCPGPCVATVDATEYAGTINEVRDFLSGRDEKVLKDIENRIAKKVAAWD
                     FEGAQALKERHQAIKQMTQKQHVHEHFGKNRDAWAFSEGTGRVSLVVLTFRRGVLLSR
                     RLYKERFYGEGPGEAIMTFLFQYYSSRPIPDEIILSEDMEDSDVLEKYLTERKKGPVR
                     IMGPSHSGARDMVRLAVENLHEPEVLPLDESFRNALRLKAAPRRELSRRLAGIE"
BASE COUNT          398 a          529 c          495 g          269 t
ORIGIN      
        1 cgtgagggac ctgagactgg gaaagttcga cgttctcgtc ggcgtgaacc tcctgcggga
       61 aggcctcgat cttcccgagg tctcccttgt cgccatactc gacgccgaca aggaaggctt
      121 cctgcgttcc cagacctctc tcatacagac ctgcggccgg gcggcccgca acctcaacgg
      181 cagggtcatc atgttcgcgg acaacataac gcaatccatg cgccgggcca tcgacgagac
      241 cgagcggcgg cgtgccgtcc agagcgccta caacgaggag aacggtataa cccccgaggg
      301 gatccgcaag tccatcgtcg atgtcctctc gtccatctac gagaaggact actacgaggt
      361 gcccgtggac gaactggacg cgaagggact caaacccaaa cagctttcga aaatggtcaa
      421 aaaactgaag aaagagatag ccgaggctgc aaagaaatgg gacttcgaga aagcggcgct
      481 gctccgcgac aggctcctgg ccattgagaa gacggagctc accctgtgac gcccgggatc
      541 aggaccgacg gcctgcccga atcaccgggt gtctacctct tcaaggacca cgacgacggg
      601 atcatctaca taggcaaggc caggaacatc cgcgaccgcg tccgcagcta ctttcgcgac
      661 gacgtgaagg acatcaagac acggacactg gcggagacga tccatgccgt ggacttcatc
      721 ctcacagcga acgagaaaga ggcctttctc ctcgagaaca acctcatcaa acagcacgtc
      781 cccaaataca acattgacct gaaggacgac aaatcgtaca tatccctgca gctcaccatt
      841 aaggacgagt accccgccct cttcgcgacg cggaagatcg tcgacgacgg ctcgctctac
      901 ttcgggcctt acccccacgc ccgcgatgtc cgcgacatcc tgaagatcat cgagcggctc
      961 tatcccgtca ggaaatgcaa gatgaccgtc ttccgcaaga ggaagagacc ctgcatgctc
     1021 tacgaactgg gcaagtgccc gggaccctgc gtggcaaccg tcgacgcgac cgagtacgcg
     1081 ggcaccatca atgaagtgcg ggacttcctc tccgggaggg atgaaaaggt cctgaaagac
     1141 atcgagaacc gcatagcaaa gaaggtcgcc gcctgggact tcgagggggc tcaggccctg
     1201 aaggagcgcc atcaagccat caaacagatg acgcagaagc agcatgtcca cgagcacttc
     1261 ggaaagaacc gcgacgcctg ggccttctcc gagggcacgg ggagggtttc cctcgtcgtg
     1321 ctcaccttcc gccggggcgt tcttctctcg cggcgcctgt acaaggaacg tttctacggg
     1381 gaaggtccgg gagaggcgat aatgaccttt ctcttccagt actacagttc gcggcccata
     1441 cccgacgaga tcatcctctc ggaagatatg gaggacagcg acgtcctgga gaagtacctc
     1501 acggaaagga agaagggccc cgtgaggatc atggggcctt cccacagtgg cgcgagggac
     1561 atggtgcgcc tcgcggtgga gaacctccac gagccggagg tcctgcccct cgacgagtcc
     1621 ttccggaacg ccctccgcct gaaggcggcg ccgcggcggg agctgtcccg gcgcctggcg
     1681 ggcatcgagg a
//
LOCUS       DAOPOA010000585         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_77376_length_1689_cov_2.353733, whole genome shotgun sequence.
ACCESSION   DAOPOA010000585 DAOPOA010000000
VERSION     DAOPOA010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77376_length_1689_cov_2.353733"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>484
                     /locus_tag="PKJ17_10705"
     CDS             <1..>484
                     /locus_tag="PKJ17_10705"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HOD76490.1"
                     /translation="NRLVREKRLSDINVYVDSPLADKATKIYMSHPEFFDADTLNTFT
                     FRSSAGMRIHFTTTIEESQKLNRVKSGAIIIAGSGMCEGGRIRHHFKHNIWRRECSII
                     FTGFQVPGTLGRYIVDGAKRAYILGEEMAIRAKVYTIGGFSAHADQGELLEWLGAFTN
                     N"
     assembly_gap    485..494
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            655..1365
                     /locus_tag="PKJ17_10710"
     CDS             655..1365
                     /locus_tag="PKJ17_10710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOD76491.1"
                     /translation="MEGQSSLFNETDFLNYRIVRSKMRKRTMTLKVERNGAVVILVPE
                     RTPDEEISRFFRSKVPWIARKLEEYKDVFGETGPRRYVTGERFLYLGEEYPLEVIEGR
                     SAKLTLYRGTFRLCNDHNADGREMFREWYRQRARQIFPERVAFYGRRLDLTWKGIRIT
                     SARTRYGSCSSDNRLSFSFRLVMAPYDVIDYIVVHELAHIKIKNHSKRFWEYIGKVMP
                     DYGRRKEWLAKKGYLLDI"
     gene            1386..>1689
                     /locus_tag="PKJ17_10715"
     CDS             1386..>1689
                     /locus_tag="PKJ17_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76492.1"
                     /translation="MRKIMIPMAVAVVVLVLGVMDVHGQTPKKQSVGKPKSGKSQIVI
                     EFVSFPDQYKRYEVYPWETLKVDDFQEAYTAMTQEGKFEDWVKSLSGTAQGKNRMIR"
BASE COUNT          400 a          449 c          522 g          308 t
ORIGIN      
        1 aacaggctgg tgagggaaaa gcgtctcagc gacatcaacg tctacgtgga cagccccctt
       61 gccgataagg ccacgaagat ctacatgtct catccggagt tcttcgatgc cgacacattg
      121 aacaccttca cattcaggag ttcggcgggg atgaggatcc acttcaccac gaccatagag
      181 gaatcgcaga agctcaaccg cgtcaaatcc ggcgccatca tcatcgcggg cagcgggatg
      241 tgcgaggggg gcaggatccg ccatcacttc aagcacaaca tctggaggcg ggagtgcagc
      301 atcatcttca ccggtttcca ggtgcccgga acactcggcc gatacatcgt cgacggcgcg
      361 aagagggcct atatcctcgg agaggagatg gccattcggg caaaggtgta caccatcggc
      421 gggttctcgg cccatgccga ccagggggag ctcctggagt ggctcggtgc cttcacgaac
      481 aaccnnnnnn nnnnggtctt tatcgtccac ggcgaggacc ctgttgccct ggagttcgaa
      541 aagacggtga gggaaaaact ggggctcacc acgtatgtgc cccacccggg agaggaactg
      601 gagatctgaa aagcccggac tggtccggga aagctgcaag acgtcagagg gaacgtggaa
      661 gggcagtctt ctctctttaa cgaaacggac tttctcaact accgcatcgt ccgcagcaag
      721 atgcgcaaac gaacgatgac gctgaaggtg gagaggaacg gagcggtcgt catccttgtt
      781 cccgaaagga cgcccgatga agagataagc cggttcttca ggtcgaaagt tccctggata
      841 gcgaggaagc tcgaggaata caaggacgtc tttggcgaga cggggcccag gcgctacgtg
      901 acgggagagc ggttcctcta tctcggtgaa gagtacccgc tggaggtcat cgaggggagg
      961 tcggcaaagc tgacccttta ccgcggcacg ttccggctgt gcaacgacca caacgccgac
     1021 ggccgcgaga tgttccggga atggtaccgg cagagggctc ggcagatctt ccccgaacgt
     1081 gtcgctttct acggccggcg ccttgacctc acgtggaagg ggatacggat aacgagcgcg
     1141 cggacgcgct acggttcctg ctcttctgac aacagactct ccttcagctt tcgacttgtc
     1201 atggccccct atgacgtcat cgactatatc gtggtccatg aacttgcgca cataaagatc
     1261 aaaaaccatt cgaagcggtt ctgggagtac atcgggaagg tgatgccgga ctacgggagg
     1321 cgcaaggaat ggcttgcgaa aaagggttat ctgctggaca tatagcggtc cggcgaggag
     1381 acgcgatgag aaagatcatg atcccgatgg cggtagccgt tgtcgtcctt gttctcggtg
     1441 tcatggacgt ccatggccag acgccgaaaa agcaatccgt tggaaagccc aagagcggaa
     1501 agtcgcagat cgtgatcgaa tttgtctcct tccccgacca gtacaagagg tatgaggtct
     1561 acccgtggga gacgctcaag gtggacgatt ttcaggaggc ctacacggcg atgacccagg
     1621 agggaaagtt cgaggattgg gtaaagtccc tctcgggcac cgcccagggc aagaaccgga
     1681 tgatacggg
//
LOCUS       DAOPOA010000586         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_77502_length_1687_cov_2.409314, whole genome shotgun sequence.
ACCESSION   DAOPOA010000586 DAOPOA010000000
VERSION     DAOPOA010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77502_length_1687_cov_2.409314"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKJ17_10720"
     CDS             complement(<1..551)
                     /locus_tag="PKJ17_10720"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOD76493.1"
                     /translation="MSVAKHLKKKFITGLFVLIPLIVTAYIIYVVVSFIEGFIGPALK
                     NIFFQVFQREVYVTGTACIIFVVITYLTGLFVSNYFGKTLFSRGERLVKRIPIVKSIY
                     GSVKDMMEAFSSEKIKSFKEVVLIEFPFQGRYAIGLVTNRVEIGGIKHCAVFVPTTPN
                     PTSGYIIITKEEELLFLDMATDDA"
     gene            complement(674..>1687)
                     /locus_tag="PKJ17_10725"
     CDS             complement(674..>1687)
                     /locus_tag="PKJ17_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76494.1"
                     /translation="DDEGGYFTWTEEDLRRTLGDEECRVLFPHLFDARNAVHHDPRKV
                     VLSVRGSVEDIARKTGMDAPTVREVIARGKQKLLAEREKRQKPFIDTALYTSLNGMMI
                     SAYCKAYRTFGDRTVLDRARLALARILDVNLRDGELYHSEGVKAFLEDYAYFCDGLIS
                     VYEATGERKYLDEAKVLMDICIDRFRDSAGAGFFDTEEEVIGIRLKGIEDIPRPSPNA
                     VAILVLIKLAAISGDDMYRDHAAKALEAFSSEASPMAIHGAYCLCALEAFYRMVKLEL
                     RAGAVSELAEASLFSYHPYTCITWGDPGDNIIIPCIATTCYEPLTSAEALKEFLEARE
                     PGI"
BASE COUNT          364 a          459 c          492 g          372 t
ORIGIN      
        1 gcgtcgtcag ttgccatgtc gagaaagagg agttcttcct ccttcgtgat gatgatgtac
       61 cccgatgtgg ggttcggcgt ggtgggcacg aaaaccgcgc aatgcttgat ccccccgatc
      121 tccaccctgt tcgtcacaag ccctatcgcg tacctcccct gaaaagggaa ttcgatgagg
      181 acgacctcct tgaacgactt tatcttctcc gacgagaagg cctccatcat gtccttgacg
      241 gagccgtaga tgctcttcac gatggggatc ctcttcacca gtctttctcc ccgtgagaaa
      301 agggtcttcc cgaagtagtt ggagacgaaa agaccggtga ggtacgtaat gacgacgaag
      361 atgatgcagg ctgtgcccgt tacgtagacc tcgcgttgaa agacctggaa aaagatgttc
      421 ttcagggcag gcccgatgaa gccttcgatg aaggaaacga caacgtagat gatataggcg
      481 gtgactatga gcggaatgag gacgaaaagc ccggtaatga actttttttt caggtgcttt
      541 gccacactca tggtgtcgac atactatcgt cggaagggag gaaagtcaat gaaaagacca
      601 tttgaatggc ttgagccgct tgaatcgctt gagccgtttc aagacaagaa aaaagacaat
      661 actttacctt gctttatatg ccgggttccc tggcctccag gaactctttc aatgcctcgg
      721 cggaggtgag gggttcgtag caggtggtgg cgatgcaggg gatgatgatg ttatcgccgg
      781 ggtcgcccca ggtgatgcac gtataagggt gataagagaa gagggaggct tcggcaagtt
      841 ccgacacagc cccggcgcgg agctcgagct ttaccatccg gtagaaggcc tcgagggcgc
      901 aaagacaata ggctccatgg atcgccatag gggatgcctc ggacgaaaag gcttcgaggg
      961 ccttcgcagc gtggtcgcgg tacatgtcgt cgcctgatat cgccgcgagc ttgatgagca
     1021 caaggatggc aacggcgtta ggtgatggtc tcggtatgtc ctcgatgccc ttgaggcgga
     1081 tccctatcac ctcttcttcg gtgtcgaaga accccgcccc cgcgctgtcc cggaaacggt
     1141 cgatgcagat gtccatgaga acctttgcct cgtcgaggta tttcctctca cccgtcgcct
     1201 cgtagacgga gatgagacca tcgcagaagt acgcgtaatc ctcgaggaag gccttcacac
     1261 cctcggagtg atagagttct ccgtcccgca ggttcacgtc caggatcctc gcaagcgcga
     1321 ggcgggcgcg gtcaagcacc gtcctgtcac caaatgtcct gtaagctttg cagtaggccg
     1381 atatcatcat gccgttgagg gacgtgtaca gcgcggtgtc gatgaagggt ttctgtctct
     1441 tctcacgctc ggcgagaagc ttctgttttc cgcgcgctat aacctcccgc accgtcggtg
     1501 catccatgcc cgttttgcgg gcgatgtctt cgacggaccc gcggacggag agcaccacct
     1561 tccgcgggtc gtggtgcacc gcgttgcggg catcgaaaag atgcgggaac agcacccggc
     1621 actcttcatc gccgagtgtc cgcctgaggt cctcctcggt ccacgtgaag tatccccctt
     1681 catcgtc
//
LOCUS       DAOPOA010000587         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_77546_length_1686_cov_2.743102, whole genome shotgun sequence.
ACCESSION   DAOPOA010000587 DAOPOA010000000
VERSION     DAOPOA010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77546_length_1686_cov_2.743102"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PKJ17_10730"
     CDS             <1..964
                     /locus_tag="PKJ17_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76495.1"
                     /translation="PRKEWEGILAKKNLSEITLRDRYSGVVHLLTAADGAPEHYSGDG
                     NPARLESTMEDAIKADTRTRNAWLGHPRFVVVDNSTCFEEKIKKTLNIILHHLGYPAQ
                     VGEERRFLVRDVDFDAMPEHRKIEIEQTYLTMRTAGQETRLKRRGQDGSCMYFIVRRD
                     LTAKRGGGFPREEEEIISREDYSKLLGMKDSRLNPVVKERFCFVWKNSHFELDFYKRR
                     NQGLVILETGLMGHDAPLPPFVEVGEEITGNREYVGKRLASRKTRSVHKPVTTRPAEK
                     ELPLEAAGKVSGDADVTEATVIPLQRSADTSPEEAQVALGHGGP"
     gene            1003..1296
                     /locus_tag="PKJ17_10735"
     CDS             1003..1296
                     /locus_tag="PKJ17_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76496.1"
                     /translation="MKVLKKTIAAARTVREHSSAPELLCLAQIPMLVLCFLSVVLMAI
                     SLFRPGVVSMAWADIPIILVDFILFGIVLMTARPPRLVASARDDESHGRTAPR"
BASE COUNT          386 a          456 c          537 g          307 t
ORIGIN      
        1 cccgaggaag gaatgggaag ggatcctggc gaagaagaac ctttccgaga taaccctccg
       61 cgaccggtac tccggagtcg tccatctgct tacagcggcc gacggcgccc cggagcacta
      121 ttccggcgat ggcaaccccg cgaggctcga gagcaccatg gaagacgcca tcaaggcgga
      181 tacgcgtacg aggaacgcct ggctgggcca tccccggttc gtggttgtgg acaacagcac
      241 ctgcttcgag gaaaagataa agaagaccct caatatcatc ctccatcacc tgggataccc
      301 ggcacaggtc ggggaagaga ggcgcttcct tgtcagggat gtggatttcg acgccatgcc
      361 ggagcaccgg aagatagaga tcgagcagac atatctgacc atgaggaccg ccggccagga
      421 gacccgcctg aaaagacgcg gccaggatgg ctcgtgcatg tatttcatcg tgaggaggga
      481 cctgacggca aaaaggggtg gtggctttcc cagggaggaa gaagagatca tctcccggga
      541 ggactacagc aagctcctgg gcatgaagga cagccgcctg aaccctgtcg tcaaagagcg
      601 tttctgcttc gtgtggaaga acagccactt cgagctcgac ttctacaaac gccggaacca
      661 gggccttgtg atactcgaaa cggggctcat ggggcacgat gcaccgttac ccccgttcgt
      721 ggaagtcggg gaagagataa cgggtaacag ggagtatgtc gggaaaaggc tggcctcaag
      781 gaaaacaagg agcgtgcaca aacctgtgac gaccaggccg gcagagaaag agcttcccct
      841 tgaagcggca ggaaaggtgt caggtgacgc cgacgtgacg gaggcaaccg tgatcccgtt
      901 gcagaggagc gcggatacgt cgcctgagga agcgcaagtt gcactgggcc atgggggacc
      961 gtagaaggtc ctggctgaag aaccccaaca gaggaggaga tattgaaggt cctgaagaaa
     1021 acaattgcag cagcgaggac ggtgcgagag cactcgtctg cgccggagct cctatgcctc
     1081 gcccagatcc cgatgctcgt gctctgcttc ctctccgtcg ttctcatggc catctcgctg
     1141 ttccgtccgg gtgtggtttc gatggcctgg gcagacattc cgatcatcct ggtggacttc
     1201 attctctttg gcatcgtcct tatgaccgcg agacctccgc gcctggtggc gtcggcgagg
     1261 gacgatgaga gccacgggag aacagccccc cgttgaaaga aaacgtgcga aaggtgctgg
     1321 tgatcgcctg ggtgacggtc agtttcgcgt tccttgtggt tctgctgacg ccatgcatgg
     1381 tccgtgcatt tgtcgcgttg ccaggcagcg gcgtcaccgg cgccgcggat aatggtccgt
     1441 atcctggcgg gctatattgc ggggctctgg tgaacatgct ggccctgcgt gtgttcctgg
     1501 ggtctgaaat agttcggagc atcagcaggc gttctcttcc tcgccctgaa cggatgtacg
     1561 cgcctgagaa tatcgaatag ggaatatcaa ggcattggca gactcacccc cgcccccgga
     1621 taaccacact ttagcggaaa ggcagaagag acaggagaag acatgtatcc cgagagattc
     1681 gcaagg
//
LOCUS       DAOPOA010000588         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_77916_length_1680_cov_3.587692, whole genome shotgun sequence.
ACCESSION   DAOPOA010000588 DAOPOA010000000
VERSION     DAOPOA010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77916_length_1680_cov_3.587692"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..526)
                     /locus_tag="PKJ17_10740"
     CDS             complement(185..526)
                     /locus_tag="PKJ17_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76497.1"
                     /translation="MMNVRELAEVLGLEVLTGETNMDRPVRSGYTSDLLSDVIANIGD
                     DAVWITIQRHVNILGVAKLKDVSAIIIPRKLDLEGSFLEKAKQEGIAVLRGTSSAFEL
                     SALVYERLKRG"
     gene            complement(602..>1680)
                     /locus_tag="PKJ17_10745"
     CDS             complement(602..>1680)
                     /locus_tag="PKJ17_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOD76498.1"
                     /translation="DVLGAFRGIGFDEVFEVALAAEIVGYIVHEFLKDYKGKRPIFSS
                     ACPAVVRLMQIKYPDLLEQMAPFLTPMEVAARIAKEQASRRTGIPYEEIGAFFISPCP
                     AKVTEMRNPMTTKHSAVNGVIGINLIYKDIIRHLGDRSANGGPEKLQRATKFGMAWGY
                     VTGESRNIDAGTTLAVGGIHNVISLLEEIERGELKDVDFVEVQACTGGCVGGPLNIQN
                     LFVGRIRLRDLIKRFGTKEPYFTEAELQDIYLQNRFAATEPVEPRAIMTLDEDVSRAI
                     MKMERLDQITSELPGLDCGACGSPSCRALAEDIIRGIAFETDCVIKLREKVKILAKEI
                     LDLSRIVPTSMSGSSKEGDPGQGH"
BASE COUNT          313 a          543 c          453 g          371 t
ORIGIN      
        1 gcgctctccc gcctccctga catatggtgt gttctccgcc atgttatggt ccgtaacggc
       61 tatgatatcc agtcccgcct cccgcgcctt ctcgacgatc gcccggggcg acatgtcaag
      121 actgccgcag ggtgacaggg tcgagtgtat atgcaggtcg caggcaaatt ccggcaagtc
      181 ctctctaacc tctcttcaga cgttcgtaca cgagagcgga gagctcgaat gccgaagaag
      241 ttcccctgag gaccgctata ccctcctgtt tcgccttctc caggaaggat ccttccaggt
      301 ccagtttccg cgggatgatg atggcggaca cgtccttgag ctttgccact ccaagaatgt
      361 tcacatgccg ctggatcgtg atccacaccg catcgtcacc gatgttggcg atcacatccg
      421 agagcagatc ggaggtgtac cccgacctca ccggcctatc catgttcgtc tctcccgtta
      481 gcacctcaag tcccagcacc tccgcaagct cacgaacgtt catcatcccc tccctgtgga
      541 cggttatccc gcccatatat taatggtacc tccagcatcc ccctgaccgt cccgacactc
      601 gtcagtgccc ctgtccgggg tcgccctctt tcgaactgcc gctcatcgag gttggaacga
      661 tgcgggaaag atcgaggatc tcctttgcca gtatcttcac cttctcgcgc agcttgataa
      721 cacagtcggt ctcgaaggct atgccgcgga tgatgtcctc ggcaagggcc cggcagcttg
      781 gggagccgca ggcgccgcaa tcgagacccg gcagttcgct cgttatctgg tccaggcgct
      841 ccatcttcat gatcgcccgg gagacgtcct catcgagggt catgatcgcc cttggctcca
      901 cgggctccgt ggcggcaaac cggttctgaa gatagatatc ctgaagttcg gcctccgtga
      961 aatagggctc tttcgttccg aaccgcttta tgaggtcgcg gagccggata cgccccacga
     1021 agagattctg gatgttgagc ggccctccca cgcaaccgcc ggtgcacgcc tgaacctcga
     1081 caaaatcgac gtccttgagc tcgcccctct cgatctcttc caggaggctg ataacgttgt
     1141 ggatgccgcc gacggccaga gtggtgcccg cgtcaatgtt ccgggactct cccgtcacgt
     1201 atccccaggc catgccgaac ttggtggcac gctgcagttt ctcgggccca ccgtttgctg
     1261 acctgtcgcc gagatgcctg atgatatcct tatatataag gttgatccct atgacaccgt
     1321 tcactgccga gtgcttcgtc gtcatgggat ttctcatctc cgtgaccttc gccggacagg
     1381 gcgatatgaa gaatgcccct atctcctcgt aggggatgcc cgtcctgcgc gatgcctgtt
     1441 cctttgcgat gcgcgccgcc acctccatgg gtgtgaggaa gggggccatc tgctcgagaa
     1501 ggtcgggata cttgatctgc atgaggcgca ccaccgccgg gcaggcagag gagaatatgg
     1561 ggcgcttgcc cttgtaatcc ttcaggaact cgtgaacgat gtagccgacg atctccgctg
     1621 caagggcgac ctcgaagacc tcgtcgaaac cgatccccct gaaggccccg agcacatcct
//
LOCUS       DAOPOA010000589         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_78115_length_1677_cov_4.366215, whole genome shotgun sequence.
ACCESSION   DAOPOA010000589 DAOPOA010000000
VERSION     DAOPOA010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78115_length_1677_cov_4.366215"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /gene="atpA"
                     /locus_tag="PKJ17_10750"
     CDS             <1..923
                     /gene="atpA"
                     /locus_tag="PKJ17_10750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOD76499.1"
                     /translation="AIGQKRFSVARIVDLLTEYGAMEYTTVVAATASDSAPLQFLAPF
                     AGTAMGEYFRDNGKHALIVYDDLSKHAVAYRQLSLLLRRPPGREAYPGDIFYLHSRLL
                     ERSAKWDDAHGGGSLTSLPIIETQAGDVSAYIPTNVISITDGQIYLEPELFYAGVRPA
                     INVGISVSRVGGNAQIKAMKQVAGRLRLEMAQYREMAAFAKFGSDLDKATQALLARGS
                     RLTELFKQGQYVPIAVEKQVVLLYAAANGFIDTYPESALKKYEAELTRFMDSKYTNIL
                     QEIGEKKAIDASIEEKLTKALNEFKEGFTY"
     gene            936..>1677
                     /gene="atpG"
                     /locus_tag="PKJ17_10755"
     CDS             936..>1677
                     /gene="atpG"
                     /locus_tag="PKJ17_10755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOD76500.1"
                     /translation="MATLRDIKRKIGSVNSTQTITRTMKMVSASKLRRAQTDLEKIKD
                     YAVKIEELTGRVVKNMPVDAHPLLETREEIKKVLIVSIGSDRGLCGGFNVNVALTAEN
                     FSKQNRDTYERVGVYVFGRKIRDYLAKRRIDVVKSWVDIKKVDQELVDAVAEEIIELY
                     LTGEFDKVYLSYTYFASPVKQEIVFEEFIPIKTPEDVEYIDYICEPDRMEIVGSLIPR
                     YISTKIYYALVESQTSEHAARMSAMENAT"
BASE COUNT          398 a          472 c          496 g          311 t
ORIGIN      
        1 tggccatcgg ccagaagagg ttctccgtgg cgaggatcgt cgacctgctg acggagtacg
       61 gcgcgatgga gtacaccacg gtcgtcgcgg cgacggcaag cgactccgct ccgctccagt
      121 tcctcgctcc cttcgcgggc accgcgatgg gtgagtactt cagggacaac ggcaaacatg
      181 ccctcatcgt gtatgacgac ctttcaaagc atgccgtcgc gtaccgccag ctctcgctgc
      241 tcctcaggag accgccggga cgtgaggcgt accccgggga catcttctat ctccactcac
      301 gccttcttga aagatcggcg aagtgggatg acgcccatgg cggcgggtcc cttacctccc
      361 tcccgatcat cgagacccag gcgggcgacg tttccgcgta tattccgacg aacgtcattt
      421 ccattaccga cggtcagatc taccttgagc cggaattgtt ctacgccggt gtcaggcccg
      481 cgatcaacgt cggtatctcg gtatcgaggg tcggcggcaa cgcacagatc aaggcaatga
      541 agcaggtggc aggcaggttg aggctggaaa tggcccagta ccgcgagatg gcagcgtttg
      601 ccaagttcgg ttccgacctt gacaaggcca cgcaagccct ccttgcccgc ggttcacggc
      661 tgacggagct tttcaagcag ggccagtacg ttcccatcgc cgtcgagaaa caggtcgttc
      721 tcctctacgc cgcggcgaac gggttcatcg atacctatcc cgagagcgct ctcaagaaat
      781 acgaggcgga gctgacgcgc ttcatggatt ccaagtatac caatatcctc caggagatcg
      841 gggagaagaa ggccatcgac gcgtcgatcg aagagaagct tacgaaggcg ctcaacgagt
      901 tcaaggaagg atttacctac taaagggagc tttcagtggc gacgctaaga gatatcaaga
      961 gaaagatagg aagtgtcaac agcacccaga cgatcacgag gacgatgaaa atggtttccg
     1021 cctcgaaact gaggcgggcc cagacggacc tcgagaagat caaggactac gcggtgaaga
     1081 tagaagagct gaccggacgt gtcgtcaaga acatgcccgt cgatgcccat ccccttctcg
     1141 agacgagaga agagatcaag aaggtcctca tcgtttccat cggctccgac aggggcctgt
     1201 gcggcgggtt caatgtcaac gtggctctga cggcggagaa cttctcgaag cagaaccgcg
     1261 acacctatga aagagtgggt gtctacgttt tcggccggaa gatccgggat tatctcgcca
     1321 agagaagaat agatgtggtg aagagctggg tcgacatcaa gaaggtcgac caggagcttg
     1381 tggacgccgt cgccgaggag ataatagaac tgtacctgac aggcgagttc gacaaggtgt
     1441 acctctccta cacgtatttc gcgtccccgg tgaagcagga gatcgtcttc gaggaattca
     1501 tccccatcaa gacgcccgag gatgtcgagt atatcgatta catatgcgag cccgaccgca
     1561 tggagatcgt cgggtccctc atcccccgtt acataagcac gaagatatac tacgccctgg
     1621 tggaatccca gacgtcggag cacgcggccc ggatgagcgc gatggagaac gcgacga
//
LOCUS       DAOPOA010000590         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_78204_length_1676_cov_2.749537, whole genome shotgun sequence.
ACCESSION   DAOPOA010000590 DAOPOA010000000
VERSION     DAOPOA010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78204_length_1676_cov_2.749537"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PKJ17_10760"
     CDS             complement(<1..537)
                     /locus_tag="PKJ17_10760"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76501.1"
                     /translation="MVKLTVRKERSGPVRGFHPWVFSKALRSIPEGLVPGEPVELCDE
                     SGSFLASGYFNSYGQISVRLWGHEKDEPVGTDFFVRRLERAADIRRRYVERPGTNAYR
                     LINGESDLIPGLIVDRYGDHLVVQFHTRGMERWKTEVVESLVAVFGPRGIWERSDVSV
                     RNIENLGADGGYCAGSSNS"
     gene            688..1293
                     /locus_tag="PKJ17_10765"
     CDS             688..1293
                     /locus_tag="PKJ17_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOD76502.1"
                     /translation="MSRLKELRTPVFWAEGHPGVLDREGWEIEVTGLCDAPRTFTWAD
                     LRAMPKTVVDARLTSVTRFTVRGNWGGVRVLDIMNAVKAHPSVAFVRFWSIKRIYDTS
                     IPVETAVRSKTLLAYEFDGEYLEEDYGGPVRAFVPYLWGYKSAKSVIRVTLMDRYVSG
                     YWEERGYTDEAYLEAGLVRDMNDGGRIRRISEEEWKECLDD"
     gene            1387..>1676
                     /gene="trxA"
                     /locus_tag="PKJ17_10770"
     CDS             1387..>1676
                     /gene="trxA"
                     /locus_tag="PKJ17_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOD76503.1"
                     /translation="MSNARAVTDGEFRTEVLESSIPVLVDFWATWCGPCQVMGPVIDA
                     LAGEYEGKVKILKLNVDENPQTPAQYGVRGIPTLIIFKNGAESGRIVGAQPKG"
BASE COUNT          365 a          461 c          539 g          311 t
ORIGIN      
        1 ggagtttgag gaccccgcgc agtagccgcc gtcggcgccg aggttctcga tgttcctcac
       61 ggacacatcg gagcgttccc agataccccg aggcccgaat accgctacga gggactccac
      121 gacctcggtc ttccaccgtt ccataccccg ggtgtggaac tggaccacga ggtgatcacc
      181 gtacctgtcc acgataagtc ccggaatgag atcgctttca ccgttgatca ggcgataggc
      241 gttcgtgccc gggcgttcca cgtagcggcg gcggatatca gccgccctct ccaacctccg
      301 gacgaagaaa tccgttccga cgggctcgtc cttctcatgt ccccagagcc tcaccgatat
      361 ctgtccgtag gagttgaagt agcccgatgc caggaaggaa ccggactcat cacaaagctc
      421 gacgggttca cccggcacga gaccctccgg tatggacctg agcgctttcg aaaacaccca
      481 gggatggaac cctctgacgg gtccgctgcg ctcctttctt accgtgagct tgaccatggt
      541 cgcaagtata gaacaatcgc gggaaaaatg gaacaggaag aggcccagga gccatagggc
      601 atatgagggc tttgctcccg ggtggtcttt tttatttgcg gacccctgaa attcggctat
      661 gattattgtt tagggcggga ggtcgcgatg agccggttga aggagttgag aacacccgtt
      721 ttttgggccg aggggcaccc cggggtcctt gacagggaag gatgggagat agaggtgaca
      781 ggactctgtg acgcgccgcg gacattcacg tgggccgatc tcagggcgat gccgaaaacg
      841 gtcgtcgacg cccgcctgac gagcgtgacg cgcttcaccg tgcggggcaa ctggggcggg
      901 gtcagggtcc tcgacatcat gaacgccgtg aaggcgcatc cttccgttgc ttttgtgagg
      961 ttctggtcga taaagaggat ctacgacacg tccataccgg tggaaacggc cgtaaggagc
     1021 aagaccctcc tggcctacga attcgacggg gaatacctcg aggaggatta cggggggccc
     1081 gtgagggcct tcgtgcccta cctctggggc tacaagtcgg cgaagagcgt cattcgcgtc
     1141 acccttatgg accgctacgt gtcaggctac tgggaggaaa gggggtacac cgatgaggca
     1201 tacctagaag cggggcttgt gcgcgacatg aacgatggcg gaaggatacg gcgcatctcc
     1261 gaggaggaat ggaaggaatg cctcgatgac tgaacatggt tgacaacgac cggcgaaggg
     1321 cttaactatt atagaagcat tgcccgccag ggggtcctgc ggcggacaac gaacaaggag
     1381 gaaggcatga gcaacgcaag agcggtaacg gacggagagt tcagaaccga ggttctcgaa
     1441 tcgagcatcc ctgttctcgt ggatttctgg gcgacctggt gcgggccatg ccaggtgatg
     1501 ggtcccgtga tcgatgccct tgccggtgaa tatgagggca aggtgaagat cctcaagctc
     1561 aatgtcgacg agaaccccca gacgccggcg caatacgggg tccggggcat ccccacgctg
     1621 atcatcttta agaatggcgc cgaatcggga cgcatcgtcg gggcgcagcc caaggg
//
LOCUS       DAOPOA010000591         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_78357_length_1674_cov_1.731933, whole genome shotgun sequence.
ACCESSION   DAOPOA010000591 DAOPOA010000000
VERSION     DAOPOA010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78357_length_1674_cov_1.731933"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..697
                     /locus_tag="PKJ17_10775"
     CDS             77..697
                     /locus_tag="PKJ17_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOD76504.1"
                     /translation="MLRRYIALALLALLVFPFSVALAKDYVVRKIIDGETVQLESGEI
                     VRYIGVNTPELNRKEGTAEFYARQAVGYNKRLVFMKKVRLEFDTKKKDEKGNLLAYVF
                     VKKTFVNAELIRNGCARADVGGPNVRYRDSLLQAERKAKAEDKGIWSEKKEDTEKQYI
                     GNKRASTFHRPTCATVEKIPEKSKIVFHNRSDAIKIGYVPCKICKP"
     gene            676..>1674
                     /locus_tag="PKJ17_10780"
     CDS             676..>1674
                     /locus_tag="PKJ17_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD76505.1"
                     /translation="MQDLQTMTEEYLRYWGLDHHPFLLAPDGSMMCVTGQYFECFERL
                     KYAINTNKGGVVVASEDAGLGKTTILLKLIDEMKAEYGSHFRYAFVDHPTLTADQMIG
                     QITGLITGEMPAEDKLKNLTTLKNALIEARQEGGKSIIVIDEGQMLCEARSVLQELRV
                     LINLTHNNEYLHTFILSGQKALWDTLQEMPEFWQRLPVRYYFTPLRFEETRELIRYRL
                     NKAGLDVAREIFAEDAIEIIHKYSKGLPRTIIAVSDLALLNGYNDKTRKIGFKEVSKA
                     INSMSGRGESLPYIVADRGEAPKETLKAPNDTAAPAGTRSPAPALEYVSHSARETFF"
BASE COUNT          434 a          471 c          455 g          314 t
ORIGIN      
        1 gcaacggggg gcggtttttt catctgcatg tattttgttt agtggcatgt caaaatagtg
       61 tacaatcatc accaatatgc tcaggagata tatcgcctta gctctgctcg ccctgctggt
      121 cttccccttc agcgtcgccc tcgccaaaga ctatgtggtc aggaagataa tcgatgggga
      181 gaccgttcag ctcgaatcgg gggagatcgt ccggtacatc ggggtaaaca ccccggagct
      241 caacagaaag gaaggtacag ccgagttcta cgccaggcag gccgtcggtt acaacaagag
      301 gcttgtcttc atgaaaaagg tccggctgga attcgacacg aagaagaagg acgaaaaggg
      361 aaatctcctg gcctatgtct tcgttaagaa gaccttcgtc aacgccgaac tcataaggaa
      421 cggctgcgcc cgggcggatg tcggcggtcc caacgtcagg tacagggaca gcctccttca
      481 agcagaaaga aaggcgaagg ccgaggacaa ggggatatgg tcggaaaaga aagaggacac
      541 tgagaaacaa tacatcggca acaagagggc ctccactttt cacaggccaa cctgcgcgac
      601 cgtcgaaaag atccccgaaa aatcgaaaat tgtcttccat aacaggagcg atgctattaa
      661 aataggttac gtaccatgca agatctgcaa accatgaccg aggagtacct cagatactgg
      721 ggccttgacc atcatccatt cctgctcgca cccgatggaa gcatgatgtg tgtgacgggg
      781 caatacttcg agtgcttcga acgtctcaag tatgccatca acacgaacaa gggcggtgtt
      841 gtcgtcgcct ccgaggacgc gggactcggc aagaccacca tccttctcaa gctgatcgat
      901 gagatgaagg ccgagtatgg ttcccatttc cggtacgcct tcgtcgacca cccgacgctc
      961 accgccgacc agatgatcgg ccagatcaca ggcctcatta ccggtgaaat gcccgcagag
     1021 gacaagctga agaacctcac gaccctcaag aacgccctta tcgaggcgcg acaggaaggc
     1081 ggcaagagca tcatcgtgat cgatgagggg cagatgctgt gtgaggcccg cagcgtcctc
     1141 caggaactcc gggtgctcat caacctcacc cacaacaacg agtacctgca cacctttatc
     1201 ctctccggcc agaaggctct ctgggacacg ttgcaggaaa tgccggaatt ctggcaaagg
     1261 cttcccgtac ggtattactt cactccgctg aggttcgagg aaacacggga attgatcagg
     1321 tatcgtctca acaaggccgg cctcgacgtc gcccgggaga tatttgccga ggacgccatc
     1381 gagatcatcc acaagtactc caagggcctt ccccggacca tcatcgccgt gtccgatctt
     1441 gcccttctca acggttataa cgacaagacc cggaagatag gcttcaagga agtctccaag
     1501 gccatcaact ccatgagcgg gaggggagag agcctgccct acatcgttgc cgacaggggg
     1561 gaggcgccga aagagacctt gaaagcaccg aacgataccg ccgcgccggc tggaacgcgc
     1621 agtccggcac ctgctctgga atatgtttcg cacagcgccc gcgagacctt cttc
//
LOCUS       DAOPOA010000592         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_78479_length_1672_cov_2.594929, whole genome shotgun sequence.
ACCESSION   DAOPOA010000592 DAOPOA010000000
VERSION     DAOPOA010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78479_length_1672_cov_2.594929"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PKJ17_10785"
     CDS             <1..757
                     /locus_tag="PKJ17_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HOD76506.1"
                     /translation="AMTPGMVGADLANLVNEAALIAARRDKKAVEMDDFEHAKDKVLM
                     GVERRSMIIPLEERRLTAYHEAGHALVAKMIPGTDPIHKVTIIPRGRALGLTQQLPID
                     ERHTYSKDYLLNNISILLGGRVAEELVIGQLTTGSGNDIERATQIARKMVCEWGMSDK
                     LGPLNYGKNEEHIFLGREIARHRDFSEQTAQGIDEELKELVGRCYSQAKDIISKHISS
                     LHALAKALLEKEVLDGLEIDAIIRDTDGVGVPV"
     gene            735..1553
                     /gene="folP"
                     /locus_tag="PKJ17_10790"
     CDS             735..1553
                     /gene="folP"
                     /locus_tag="PKJ17_10790"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOD76507.1"
                     /translation="MAWAFQCKTTPVIMGILNVTPDSFSDGGRHRSTEDAVDHARLMI
                     EEGADIIDVGGESTRPFSEPVAEKEEIRRVIPVIERLHEIPGAFISVDTYKANVAREA
                     LKAGADMVNDISGLTYDDAMAGVIAGNDAHAVIMHIKGTPRNMQENPWYDDVVGEISA
                     FLHERVEYAVRSGVDREKIVIDPGIGFGKRVEDNLRILKMLRAFHELGRPVLIGTSMK
                     SFIGKLTDMPLEERVEGTLASLAVAIMNGADVLRVHDVRGAKKVLKIVKAVMDA"
BASE COUNT          396 a          499 c          505 g          272 t
ORIGIN      
        1 cgccatgacg ccgggcatgg tgggcgccga cctcgccaac ctcgtcaacg aggccgccct
       61 catagccgcg cggcgtgaca aaaaggccgt cgaaatggac gacttcgagc acgccaagga
      121 caaggtcctc atgggggtcg agaggcgcag tatgataatc cccctggagg aaagacggct
      181 gaccgcctat cacgaggcag gccacgccct cgtggcaaag atgatccccg gcaccgaccc
      241 gatacacaag gtcacgatca tccccagggg cagggcgctg gggctcacgc agcagttgcc
      301 catagacgag cgccatacgt actccaagga ttaccttctc aacaacatct ccatcctcct
      361 cggagggcgc gtcgcggagg aactcgtcat cggtcagctc acaacggggt cgggcaacga
      421 catcgaacgc gccacccaga tcgcccggaa gatggtctgc gagtggggca tgagcgacaa
      481 actgggaccc ctcaactacg gcaagaacga agagcacata ttcttgggca gggagatcgc
      541 ccgccaccgg gatttcagcg aacagaccgc tcagggcatc gatgaagaac tgaaggagct
      601 cgtcggcagg tgttactcac aggccaaaga catcatctcc aagcacatca gctcccttca
      661 cgccctggcg aaggcactcc tcgaaaagga agtcctcgac ggcctggaga tcgatgctat
      721 catcagggat accgatggcg tgggcgttcc agtgtaagac aacccccgtc atcatgggga
      781 tcctcaatgt cacccctgac tctttttccg atgggggcag acaccgaagc acggaggatg
      841 ccgtcgacca cgcccggctg atgatcgagg aaggcgcgga catcatcgac gtcggcggag
      901 aatcgacccg gcccttttca gaacctgtag ccgaaaaaga agagatccgt cgggtaatac
      961 ctgttataga gaggcttcac gagatacccg gcgcgttcat ctccgtcgat acgtacaagg
     1021 cgaacgtggc ccgggaagcc ctcaaggcgg gagccgacat ggtcaacgac atcagcggcc
     1081 tcacgtacga cgacgccatg gcaggggtca tcgccgggaa tgacgcccac gcggtcatca
     1141 tgcacatcaa gggcacgcca cgcaacatgc aggagaaccc ctggtacgat gacgtcgtcg
     1201 gcgagatcag cgcgttcctc catgaacggg tggaatacgc cgtgcggagc ggggtcgaca
     1261 gggagaagat cgtcatcgac ccgggcatcg gtttcgggaa acgggtggag gacaacctgc
     1321 gcatcctcaa gatgctcagg gccttccatg aactgggcag gcccgtcctc atcggcacct
     1381 cgatgaagtc tttcataggg aagctgacgg acatgcccct tgaagagagg gtggagggga
     1441 ccctggcaag cctcgccgtc gcgatcatga acggggctga tgtgctgagg gtccatgatg
     1501 tgaggggcgc gaaaaaggtc ctcaagatcg tgaaggcggt aatggacgca tgatacccaa
     1561 cgtcagatgg caggacatcc tcgatatcat tgtcgtcgcc ttcatcatct accgcatctt
     1621 cgtcgtcata aaggggacgc gggcaataca gctccttctc ggcctcgtga tc
//
LOCUS       DAOPOA010000593         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_78662_length_1669_cov_2.990087, whole genome shotgun sequence.
ACCESSION   DAOPOA010000593 DAOPOA010000000
VERSION     DAOPOA010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78662_length_1669_cov_2.990087"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PKJ17_10795"
     CDS             complement(<1..616)
                     /locus_tag="PKJ17_10795"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOD76508.1"
                     /translation="MRKLVIKKDKTIRQKVYHHIREEILKGAIAPKERLIEAKIAGEI
                     GTSRTPVREALHNLELEKLVVSIPRVGYVVRGMDMEEVEQICEIRAAIESLAIAWATA
                     KQRDRLIGALKRNIENQKKQISKGNLNGYVELDAQFHDIIARLAGSDRLLELTQTLRR
                     HMLRYRIQCIYMTETAERSMHGHERIVTAIEGGDSQEIAAAVKTH"
     gene            669..>1669
                     /locus_tag="PKJ17_10800"
     CDS             669..>1669
                     /locus_tag="PKJ17_10800"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOD76509.1"
                     /translation="MKISLPHGLWNGERTLEADFPARWDVDVLSMEGDRARPIGGAEY
                     RAAIMPLVPLMKGAKEVCVLFDDLSRPTRTYEIVPTLLEALAGADIDDSRVRFLCALG
                     THSPHNNADFRKKLGNEVLQRFPVYNHNCYENCETLGRTALGTPVTINKEYLTCDLRI
                     GMGSFIPHQFCGFGGGYKMVFPGIAHIDAIQYHHGLLLQQNLESCWGLGNHSCNAILS
                     DMREAGRMARLDIKIDVLVNSFARATRVFAGYPDSLYPAMIEGALRHYATKPRGEYDI
                     VFANTFGKANEAVIAASTSEEILTPDGGYLVILNDIEEGQVIHYLMGRFGKDLWGRLG
                     "
BASE COUNT          310 a          532 c          456 g          371 t
ORIGIN      
        1 ggtgtgtctt caccgcggcg gcgatctcct gagagtcccc cccttcgatg gcggtcacga
       61 tccgttcgtg gccgtgcatc gatctctccg ccgtctcggt catgtagatg cactggatcc
      121 ggtacctcag catatgcctg cgcagggtct gggtgagctc cagcaggcgg tcgctgccgg
      181 caaggcgcgc aatgatgtcg tggaactgcg cgtccagctc aacgtagccg ttgagattgc
      241 ctttcgatat ctgtttcttc tggttctcga tgttcctctt gagggccccg atgagcctgt
      301 ccctttgctt cgccgtggcc cacgcgatgg caagactttc gatcgccgcc ctgatctcgc
      361 atatctgctc cacctcttcc atgtccatgc ccctgaccac gtagcccacc cgggggatgg
      421 agacaacgag cttctccagt tcgaggttgt ggagcgcctc gcgcacggga gtccgggatg
      481 tgcctatctc cccggcgatc ttcgcttcta taagcctctc cttgggggcg atggcacctt
      541 tgaggatctc ctcgcgtatg tgatgataga ccttctgccg tatcgtcttg tcttttttta
      601 ttaccagttt tctcattaat ttcgtatact gtaccctagt tgttgttatc attacaagtt
      661 ctaaatttat gaaaatatcc ctgccccacg gcctctggaa cggtgagcgc accctcgagg
      721 cggacttccc ggcccgttgg gacgtggacg tgctgtcaat ggaaggtgac agggcacggc
      781 ccatcggcgg agcggaatac cgggccgcca tcatgcccct cgtcccgctc atgaaaggcg
      841 cgaaggaggt ctgcgtcctc ttcgacgacc tctcccggcc gacacgcacc tacgagatag
      901 tcccgacgct cctcgaggcc ctggcaggcg cggacatcga cgactcccgg gtgcggttcc
      961 tctgcgccct gggcacccac tcccctcaca ataacgccga tttcagaaag aagctcggca
     1021 acgaggtgct tcagcgattt cccgtttaca accacaactg ctacgagaat tgcgaaacgc
     1081 tgggaaggac ggccctggga acacccgtta ccattaataa agaatacctg acctgcgacc
     1141 tcaggatagg catgggctcc ttcatccccc accagttctg cggtttcgga ggcggataca
     1201 agatggtctt tcccggcata gcccacatcg atgccattca gtatcaccac ggtctccttc
     1261 tccagcagaa cctggagagc tgctggggcc tcggcaacca ttcctgcaac gccatcctct
     1321 cggacatgcg cgaggcggga aggatggcgc ggctcgacat caagatcgat gtcctcgtga
     1381 acagtttcgc ccgcgccacc cgcgtcttcg ccggctatcc cgattccctg tatcccgcca
     1441 tgatagaggg ggcgctcagg cattacgcga cgaaaccccg gggagaatac gacatcgtct
     1501 ttgccaacac cttcggcaag gccaacgagg ccgtcatcgc cgcttccacg tcggaagaga
     1561 tccttacccc cgacggcggc tatcttgtca tcttgaacga catcgaggag gggcaggtca
     1621 tccactacct catggggagg ttcggaaagg acctctgggg caggctcgg
//
LOCUS       DAOPOA010000594         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_78834_length_1667_cov_2.033499, whole genome shotgun sequence.
ACCESSION   DAOPOA010000594 DAOPOA010000000
VERSION     DAOPOA010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78834_length_1667_cov_2.033499"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKJ17_10805"
     CDS             <1..208
                     /locus_tag="PKJ17_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76510.1"
                     /translation="ETIDRYFNLSEEQRLIYRFGRRSGIYRYLDDLGDELACFRIRKS
                     IREMEEKEPGSVEKAISLLLENYI"
     gene            506..>1667
                     /locus_tag="PKJ17_10810"
     CDS             506..>1667
                     /locus_tag="PKJ17_10810"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOD76511.1"
                     /translation="MIASSGVVLSSLLTHRSASKRLGSAAKTEMERTAALIARQSETT
                     LDIFASGIRSLAREDCVRRAILRPGAAAVIDEVCGRFREIAHEAKVYQSINLHDREGR
                     CVASSFPDRVGLESMQRDVVARPDFLSAMKGEAVVSQIFVSRGTGRPVIVISAPVEIG
                     GAVPGVVRAVVDLTYFNDYILTPQDYVHGGKAYIFDPKLDREVPDGWKPHNIIEARAY
                     EQPGIPIPEDMLRRGNGFVRYPSARGEQLAAFCRTPRPEWFFVVEKPRKDTMRPIHAM
                     GRVTAGTLISMLFGVWGGALAVARPLLTRLGQCMDMARDIEMGRLDRRVEVRGSDEVA
                     RLGKGLNAMAHSLEESHAALEEAERLYRGIFENAMEGIFIMDPGGLLVNVNPA"
BASE COUNT          336 a          470 c          518 g          343 t
ORIGIN      
        1 cgagaccatc gaccgctatt tcaacctctc cgaggagcaa aggctgatct accgtttcgg
       61 caggcgttcc ggcatatatc gataccttga cgacctgggg gacgaactcg cctgttttcg
      121 catcagaaaa tccatccggg aaatggaaga aaaggaaccc ggcagcgttg agaaggccat
      181 ttccctcctc ctcgaaaatt acatctagac cagaagaccg tttcatagga ataaggctgt
      241 tacgggttac gggttccggg tcaaagacca aaagagcata gcaaggcgca aggatgcgat
      301 gcactggtca ataattgcgt tgacaaccga tctccttccg cagtcttttt ctggcaaccc
      361 gcaacctatt tttgtttatt gacatttgac gatggtccgt gagagtattt ttcctgtcgt
      421 tgccatgaaa aagggggctt ttgggcttcg gctttgtctc tcgtcaatga tgacctttcg
      481 cggaaaactt ctatttgtgt tctcgatgat cgcttcgtcg ggggtggtgt tgtcctctct
      541 gctcacacac aggtcggcaa gcaaacgact cgggtcggcg gcgaagacgg agatggagcg
      601 cacagcggcg ctgatcgcca ggcagagcga gaccaccctc gatatcttcg cttccggcat
      661 ccggtcgctg gcccgtgagg actgtgttcg ccgggcgatc cttcgtccag gggccgccgc
      721 tgtgatcgac gaggtttgcg ggcgcttccg ggagatcgct cacgaggcga aggtgtacca
      781 gtcgataaac cttcatgatc gcgagggccg ctgtgtggcc tcatcgttcc cggaccgtgt
      841 gggcctcgag agcatgcaga gggacgtggt ggcacgtcct gatttccttt ccgccatgaa
      901 gggagaggcg gtcgtgtccc agatctttgt cagccgggga acgggccggc ccgtcatagt
      961 catctcggcc cccgttgaga tcggcggcgc cgttcccggt gtcgtgcgcg cggtcgtgga
     1021 cctcacctac ttcaacgact acattctgac cccccaggac tatgttcacg ggggaaaggc
     1081 ctacatcttc gacccgaagc tcgacaggga ggtccccgac ggctggaagc cccacaatat
     1141 catcgaggcg agggcatacg aacagccggg catccccatc cccgaggaca tgcttcgccg
     1201 cgggaacgga ttcgtccgct acccgtcggc gcggggtgag caactggcgg cgttctgcag
     1261 gacaccgcgg cccgaatggt tcttcgttgt agaaaaaccg cggaaggaca ccatgagacc
     1321 catacacgcc atgggaaggg tgaccgcggg gacgctcatc agcatgctct tcggtgtgtg
     1381 ggggggagct ctcgccgtgg cgcgcccgct gctcacgaga ctcggccagt gcatggacat
     1441 ggcccgcgat atcgagatgg gacgtctcga ccggcgtgtg gaggtcaggg gcagcgatga
     1501 ggtagcccgc ctgggaaagg ggctcaatgc gatggcccac agcctcgagg agagtcacgc
     1561 ggcgcttgaa gaggctgaac gcctgtacag gggtatcttc gagaatgcga tggaagggat
     1621 attcatcatg gaccccggcg gtcttctcgt gaacgtaaac ccggcct
//
LOCUS       DAOPOA010000595         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_79139_length_1662_cov_3.461730, whole genome shotgun sequence.
ACCESSION   DAOPOA010000595 DAOPOA010000000
VERSION     DAOPOA010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79139_length_1662_cov_3.461730"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /gene="plsX"
                     /locus_tag="PKJ17_10815"
     CDS             complement(<1..903)
                     /gene="plsX"
                     /locus_tag="PKJ17_10815"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOD76512.1"
                     /translation="MIRIAVDGMGGDFAPREIVRGAQSISREGLASIVLVGDEGKMRP
                     FVTDHANLDLVHTPVCVEMNEAPSNALRKKRDSSMNRAFELHKNGEVQAVVSAGNSGA
                     AMAFAIFTLGRIPGVDRPAIATLHPRMGEGVSILLDAGGTVDCKPTHLAQFALMGNAF
                     ASSVLGIASPRVGILSNGEEETKGNELTREAHALIKEFSLNYLGYVEGTDMYNGRTDV
                     VVSDGFVGNIALKISEGVADLIFEFFREGIKKSRKAQMGYFLLKDLFKEFQKKADYSE
                     TGGAPLLGVDGVCIICHGKSNEKAM"
     gene            complement(912..1094)
                     /gene="rpmF"
                     /locus_tag="PKJ17_10820"
     CDS             complement(912..1094)
                     /gene="rpmF"
                     /locus_tag="PKJ17_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOD76513.1"
                     /translation="MAVPKRKTAKARRDKRRAHYTASPVAVVACPNCKEPKLPHAVCP
                     KCGMYKGKQYIAVEEA"
     gene            complement(1108..1623)
                     /locus_tag="PKJ17_10825"
     CDS             complement(1108..1623)
                     /locus_tag="PKJ17_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOD76514.1"
                     /translation="MLIRLTEIEDILVVKGEMDTARFPKTDDAEFTLLEPVRYELTVA
                     KFDDTVSIEGPVTFKASLACSRCLESYSLSMSLQMAIKLTPKSMLPDAAEMELHNDDL
                     DVYYYEGDEIDLDPFIYEEVMLNIPVRPLCSEECKGICPTCGRNRNAEPCDCPEAPSS
                     LLGEKLKSFLN"
BASE COUNT          302 a          485 c          477 g          398 t
ORIGIN      
        1 catggccttt tcgttcgatt tcccatgaca tatgatgcag accccgtcga cgccaagaag
       61 aggcgccccc ccggtctcgg aataatccgc cttcttctgg aactctttga agaggtcttt
      121 cagaaggaaa taccccatct gtgcctttcg gcttttcttt attccctccc tgaagaactc
      181 gaagatcagg tccgcgaccc cctcgcttat cttcagggcg atgtttccga caaaaccgtc
      241 gctcaccacg acatcggtcc tgccgttgta catgtcggtg ccttccacgt accccaggta
      301 gttgaggctg aactccttga taagggcatg ggcctcccgg gtcagttcgt tgccctttgt
      361 ctcctcctcg ccattcgaaa ggatgcccac ccggggggaa gcgatcccca ggacggaaga
      421 ggcgaaggcg ttgcccatca gggcgaactg ggcgaggtgc gtgggcttgc agtccacggt
      481 gccgcccgcg tcgagaagga tcgaaacccc ttctcccatg cgcgggtgca gcgtggctat
      541 cgcgggccgg tcgacgccgg ggatccggcc gagggtgaat atggcaaagg ccatggccgc
      601 cccggaattt cccgccgaaa cgacggcctg gacctctccg ttcttgtgca gttcgaatgc
      661 cctgttcatg gaggagtccc tctttttcct gagggcgttc gaaggggctt cgttcatctc
      721 cacgcaaaca ggtgtgtgga ccaggtcgag gttcgcatgg tccgtcacga aaggccgcat
      781 ctttccctcg tcgccgacga gaacgatgga agccagcccc tcacgggaga tcgactgggc
      841 ccccctcacg atctccctgg gcgcaaagtc gccacccatt ccgtcgacgg cgatccgtat
      901 cattccgggc tttaagcttc ctcgaccgct atgtactgtt tgcccttgta cattccgcac
      961 ttggggcaaa cggcatgggg aagtttaggc tccttgcagt tcgggcatgc cacgacggca
     1021 acaggtgatg cggtgtagtg cgcgcgtctc ttgtcccgtc tcgccttcgc agtttttcgt
     1081 tttggtacgg ccatggtcta ctccttgtca gtttaagaaa gatttcaatt tttcgccgag
     1141 gaggctcgag ggcgcctcgg gacaatcgca gggttccgcg tttctgttcc tgccgcaggt
     1201 cgggcatatc cctttgcatt cttcgctgca cagcggcctc acggggatgt tgagcatgac
     1261 ctcttcgtag atgaagggat cgaggtcgat ctcgtcgcct tcgtagtagt agacgtcgag
     1321 gtcgtcgttg tgaagctcca tctccgccgc gtcaggaagc atgcttttgg gggtgagctt
     1381 gatggccatc tgaagggaca tcgagaggct gtagctctcg aggcagcggg aacaggccag
     1441 cgatgccttg aacgtgacgg gtccttcgat gctgacggtg tcgtcgaact ttgcgacggt
     1501 aagctcgtag cgcaccggct cgagcagcgt gaattcggca tcgtcagtct tcgggaatcg
     1561 tgcagtgtcc atctcaccct tcacgaccag tatgtcttct atttccgtaa gtcgtatcaa
     1621 cacgggtcgc cttcttttga agatagataa tataaagagt tt
//
LOCUS       DAOPOA010000596         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_79140_length_1662_cov_3.409459, whole genome shotgun sequence.
ACCESSION   DAOPOA010000596 DAOPOA010000000
VERSION     DAOPOA010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79140_length_1662_cov_3.409459"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PKJ17_10830"
     CDS             <1..888
                     /locus_tag="PKJ17_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76515.1"
                     /translation="HPTARGLRIGKKLGERGPDEPLDENGEWDRDGQYFHYLTKWMHA
                     LNLVSRSTGDFTYNRWAMELAKAAHRGFVYTVPGTRTRRMFWKMSTDLSYPLVSSMGH
                     HDPLDGLITFIQLEAMEDPDRPEHLGLAAEIDDLACICEGREWQTDDLLGLGGLLVDA
                     FRVAQLFAKGDIEGSDTLRVILDASLPGLKYPDRENFLHAPARFRLAFRELGLSIGFH
                     ALERLEVLIEQNREAFAEDRDIGILMKTLWKYLPLGGKIETFWLDAVNQKTDVWKEHR
                     DINMVMLATSLAPDGFLTL"
     gene            complement(914..>1662)
                     /locus_tag="PKJ17_10835"
     CDS             complement(914..>1662)
                     /locus_tag="PKJ17_10835"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOD76516.1"
                     /translation="PKYLCGICFTIKEIQDMTSATLKLLACALMVVDHLGSIFFPHEP
                     VMRAIGRLAYPMFAYLIAEGYSHSKDKTSYLGRLFLFSLVSQPFYMFAFRYPVVHFNV
                     FFTLAAGLYAIYAYEKKKSLVPVLLAGISLEFVKASYGLPGALMIFVMHYFMGNYRKM
                     FLWVLITAALCTLRGIAWRYFDDPHFMPTLAWILPHARVSLMEPLAALAVPIIALYNG
                     KLGPKMKFFFYAFYPGHLAILGAIRWLLAR"
BASE COUNT          367 a          465 c          536 g          294 t
ORIGIN      
        1 catccgacgg cgagaggcct gaggatcggc aagaaactcg gcgagcgcgg ccccgacgag
       61 cccctcgatg agaacgggga atgggacagg gatgggcagt actttcacta cctcacgaag
      121 tggatgcatg cgctcaacct ggtctcccgc tcgacgggcg acttcacata caaccggtgg
      181 gcgatggaac tcgcgaaagc cgcgcaccgc gggtttgtct acaccgttcc cggaacgcgg
      241 acgaggcgga tgttctggaa gatgagcacc gatctttcct atccccttgt ctcctccatg
      301 gggcaccacg atccgctcga cggtctgatc acctttatcc agctcgaggc tatggaggac
      361 cccgacaggc cggaacacct cggcctcgcg gccgagatcg acgaccttgc ctgtatctgt
      421 gagggaaggg aatggcagac ggacgacctt ctcggcctcg ggggcctgct cgtcgacgcc
      481 ttccgggtgg cgcagctctt tgcgaagggc gatatcgagg ggtccgacac gctccgggtg
      541 atcctcgacg cctccctccc gggcctcaag taccccgaca gggagaactt cctccacgca
      601 cccgcccggt tccgtcttgc ctttcgggaa ctggggttgt cgatcgggtt ccacgccctg
      661 gaaagattgg aggtgctcat cgaacagaac cgggaggcct tcgccgagga ccgtgacatc
      721 ggcatcctca tgaagaccct ctggaagtac ctgcccctgg gcgggaagat cgagaccttc
      781 tggctcgacg cggtcaatca gaagaccgac gtgtggaagg aacaccgcga cataaacatg
      841 gtcatgctcg caacaagcct cgccccggac ggcttcctaa ctctttagca cggttcaagc
      901 ggctcaagcc gggtcatctg gccagcagcc accgtatagc gccgagtatg gccaggtggc
      961 cggggtagaa ggcgtagaag aagaatttca tctttggccc cagttttccg ttgtacaggg
     1021 cgattatggg gactgcgagc gcggccaggg gctccatgag cgagacacgg gcatggggca
     1081 ggatccaggc gagggtgggc atgaaatggg ggtcgtcaaa gtatcgccag gctatgcccc
     1141 tcagggtgca gagcgcggcg gtgatgagga cccagaggaa catcttcctg taattcccca
     1201 tgaagtaatg catgacgaag atcatgaggg cccccggcag gccgtaggag gccttcacga
     1261 actccaggga tatgccggcg agaagaacgg gcacgaggct cttcttcttc tcgtaggcat
     1321 atatcgcgta aagccccgcg gcaagggtga agaagacgtt gaaatgcaca acgggatagc
     1381 ggaaggcgaa catgtagaag ggctgggaga caagggagaa gagaaagagg cggccgagat
     1441 aggaggtctt gtccttcgag tggctatagc cctcggcgat gagataggcg aacatgggat
     1501 aggccagtct gccgatggcc cgcatcaccg gttcgtgggg gaagaagatg gacccgaggt
     1561 ggtcgaccac catgagggcg catgccagaa gcttcagtgt agctgaggtc atatcctgta
     1621 tctccttgat cgtgaagcaa atgccacaaa gatatttcgg aa
//
LOCUS       DAOPOA010000597         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_79443_length_1658_cov_2.079850, whole genome shotgun sequence.
ACCESSION   DAOPOA010000597 DAOPOA010000000
VERSION     DAOPOA010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79443_length_1658_cov_2.079850"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /gene="nifS"
                     /locus_tag="PKJ17_10840"
     CDS             <1..1113
                     /gene="nifS"
                     /locus_tag="PKJ17_10840"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOD76517.1"
                     /translation="PFFHDRYGNPSSMHSFGGQVARELSQARARVASLLGVDPEEIIF
                     TSCGTESDNTAIMSTLLVNPGKTHIVTSRVEHPAIKVLCEHLAQKGYRVTMVGVDSDG
                     RLDMDEYEASLTPDTAIASIMWANNETGVVFPVEEAARLARERGIVFHTDAVQAVGKI
                     PIDMKDNAIDMLSLSGHKLHAPKGVGVLYVRKGTKFSPFLIGGHQEKGRRGGTENVPG
                     IIGLGKACELAGANMEKENTYVRNLRDLLESGLLTAIAQSRVNGVQDERLPNTTNISF
                     EFVEGESILLLMDQFGICASSGSACTSGSLQPSHVLRAMGVPYTMAHGSIRFSLSVYN
                     TEEDIDLVIKELPPIIEKLRVLSPYWATQQGACTTV"
     gene            1124..>1658
                     /locus_tag="PKJ17_10845"
     CDS             1124..>1658
                     /locus_tag="PKJ17_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76518.1"
                     /translation="MQEGENSKDRCREEITATQNHRSRLPFLVDRIVAQYDKKGRLHH
                     VGPEPIPSRRAIIDIIRKTIPILFPGFFAEKRLDEINHRYYLGEQVTELFDAIATQIQ
                     HAIRHDCIRFNRPCVHCEDLSQEITLRFIENIPELQETLSKDVRAAFEGDPASRHFDE
                     IIFCYPGLFAVAVYRVAH"
BASE COUNT          384 a          502 c          469 g          303 t
ORIGIN      
        1 ccttttttcc atgatcggta cgggaacccc tcgagcatgc actccttcgg cggccaggtg
       61 gcccgcgaac tctcccaggc ccgtgccagg gttgcctcgc tcctcggggt tgacccggag
      121 gagatcatct tcacgagctg cggcacggaa agtgataaca cggcgatcat gtcgaccctg
      181 ctcgtaaacc cggggaagac gcacatcgtc acgagcaggg tcgaacatcc ggcgatcaag
      241 gtcctgtgcg agcaccttgc ccagaaaggg taccgcgtga caatggtcgg tgtggacagc
      301 gatggaaggc tcgatatgga cgaatacgag gcaagcctga cgccggacac ggcgatcgcg
      361 agcatcatgt gggccaataa tgaaacgggt gtggtctttc ccgtggagga agcggcacgc
      421 ctggcccgcg agcgggggat cgtcttccat accgacgcag tccaggccgt gggaaagata
      481 cccattgaca tgaaagacaa tgcgatcgac atgctttctc tttcgggtca taaactgcat
      541 gcgccgaaag gcgtcggcgt cctgtacgta cggaaaggga caaagttctc cccctttctc
      601 atcggcggcc accaggaaaa gggacgccgc ggtggcacgg agaacgtccc cgggatcatc
      661 gggctcggaa aggcctgcga acttgccggc gccaacatgg agaaggagaa cacgtacgtg
      721 agaaacctgc gcgacctgct ggagagcggg cttctcaccg caatcgcaca aagccgggtg
      781 aacggcgtcc aggatgagcg tcttcccaac acgacgaata tcagcttcga gttcgtcgaa
      841 ggtgaaagca tcctcctgct catggaccag ttcggcatat gcgcctcctc gggctccgcc
      901 tgcacctccg gttcactcca gccttctcat gtcctgcggg ccatgggcgt tccctacacg
      961 atggcccacg gatcgatacg gttcagcctc agcgtctaca atactgaaga ggatatagac
     1021 ctcgtcataa aggaactgcc tccgataatc gaaaagctca gggtcctttc accctactgg
     1081 gcgacacagc agggcgcctg cacaacggtc taaggagaga gacatgcaag agggagaaaa
     1141 ttcaaaggac cgctgcaggg aagagataac cgcgacccag aaccatcgct cacggctgcc
     1201 tttcctcgtc gacaggatag tggcacagta cgacaaaaag ggccgtctcc atcacgtcgg
     1261 ccccgagccg ataccttcac gacgggccat catcgatatc atacgcaaga ccatccccat
     1321 ccttttcccg ggtttctttg ccgagaaacg actggacgag atcaaccacc ggtattatct
     1381 gggagagcag gtgacagagc tcttcgatgc gatcgcgaca cagatacagc acgcgatacg
     1441 ccacgactgc atccggttca accggccctg cgttcattgc gaggacctca gccaggagat
     1501 aaccctgagg ttcatcgaaa acattcccga actgcaggag acgctctcga aagacgttcg
     1561 cgccgccttt gaaggcgacc cggcctcacg gcattttgac gagatcatat tctgctatcc
     1621 cggtctcttc gcggttgccg tctatcgcgt cgcccact
//
LOCUS       DAOPOA010000598         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_79624_length_1655_cov_3.016250, whole genome shotgun sequence.
ACCESSION   DAOPOA010000598 DAOPOA010000000
VERSION     DAOPOA010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79624_length_1655_cov_3.016250"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKJ17_10850"
     CDS             <1..383
                     /locus_tag="PKJ17_10850"
                     /inference="COORDINATES: protein motif:HMM:NF021883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76519.1"
                     /translation="HSSAERVMAPDATIALDYMLERVKNVFKNLLVYPEKMQKNLNIT
                     RGLYHSEAILIALIRKGLTRQEAYKVTQGVAMRCYEGGLDFVTELKKDAELAKHLSAK
                     EIEETCTNNHYFRHIETIFKRVYE"
     gene            complement(624..944)
                     /locus_tag="PKJ17_10855"
     CDS             complement(624..944)
                     /locus_tag="PKJ17_10855"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOD76520.1"
                     /translation="MVELDDIKKKVQDEVGTCEAAEEHLFPELNFSTFLLSLSTSAMV
                     CLGELPDPISNDKNTNLPLAKQTITIIEILKEKTKGNLNPDEERLIDGILYDLHIRYV
                     KAAC"
     gene            complement(954..>1655)
                     /locus_tag="PKJ17_10860"
     CDS             complement(954..>1655)
                     /locus_tag="PKJ17_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOD76521.1"
                     /translation="GVPIGIVTAIYGEGKGIGFAIPMDDVMTILSEFTESKLTRPILG
                     LFIGRQKEDKKNYLLVERVIPGSPAAKYGFMVGDRITEINKRVIRESLKIQVAIRSVK
                     SRDSFQVRLTRGGRKYTVNIDTADVEEYAPQPVDAVLCGMRVSDISGYPKLKYRLRDK
                     DGAVVVKVFPGSIAMRSGLRKGDVIVKINNNPVKNRKDFDTFMIEGLKRNYILYQVKR
                     DDSLFFAPVKLDILL"
BASE COUNT          362 a          472 c          436 g          385 t
ORIGIN      
        1 gtcattcatc ggcggagcgt gtcatggccc ccgacgccac gatcgctctc gactacatgc
       61 ttgaaagggt gaagaacgtc ttcaagaacc tcctcgtcta ccccgagaag atgcagaaga
      121 acctcaacat cacccgcggt ctctatcact cggaggcgat actcatcgcc ctcatcagga
      181 aaggcctcac gaggcaggag gcatacaagg tcacccaggg cgtggccatg cgctgctacg
      241 agggcggcct cgatttcgtc acggaattga aaaaggacgc tgaactggca aaacacctct
      301 cagcgaaaga gatcgaagag acctgcacca acaaccacta cttccgccac atcgaaacca
      361 ttttcaagag agtgtacgag taaaagacgg cttgagtcgc ttgaatggct tgagtcgttt
      421 gagagtcaaa ggcaggaacg tcagaatctg tcttgcgcct ggggcctgaa caccgttaga
      481 ttccggccat cgccggaacg acggaaagcg gggcctcaga tcgcaatcgc cctgaggtta
      541 tcaccgcaaa gcactacaag aattcgagct tctaaccctc aagccattca agcgactcaa
      601 gccattcaag ccaggccgcc tgttcagcag gcggccttta cgtatcgtat gtgcaggtcg
      661 tagaggatgc cgtcgatgag gcgctcttcg tcggggttca ggttgccctt cgttttttct
      721 ttcaggatct ctatgatcgt gatcgtctgt tttgccaggg gaaggttcgt gttcttgtcg
      781 ttgctgatcg ggtccggcag ctctccgaga cagaccattg ccgatgtgga gagcgacagc
      841 aggaacgtgg aaaagttcag ttcgggaaaa agatgctcct ccgcagcctc gcaggtaccg
      901 acctcgtcct ggaccttttt cttgatatca tccaattcca ccatttaagc cccctatagg
      961 agaatatcga gcttcaccgg tgcgaagaag agactgtcgt cccttttcac ctggtagagg
     1021 atatagttcc tcttgagccc ctctatcatg aacgtgtcga aatcctttcg gttctttacc
     1081 gggttgttgt tgatcttgac gatgacatcg ccctttcgca gtcccgacct cattgcgatg
     1141 cttccgggaa aaaccttcac caccaccgca ccgtccttgt cgcggagacg gtatttcagt
     1201 ttgggatacc ccgatatgtc ggagactctc ataccgcaaa ggaccgcgtc gacaggttgc
     1261 ggcgcgtatt cctccacgtc ggccgtgtcg atgttgacgg tgtatttcct gccaccgcgg
     1321 gtgagccgga cctggaagct gtcccgcgat ttcacggaac ggatggcgac ctggatcttc
     1381 agggattccc ggatcaccct cttgttgatc tccgtgatcc tgtctcccac catgaacccg
     1441 tatttcgcgg cggggctccc cggtatgacg cgctcgacga gaaggtagtt cttcttgtcc
     1501 tctttctgtc ttccgatgaa aaggccgaga atgggccggg tcagcttgct ttccgtgaat
     1561 tccgaaagga tggtcatcac gtcatccatg gggatggcaa accctatccc tttaccctcg
     1621 ccgtaaatgg ccgtaacgat gccgatgggg acacc
//
LOCUS       DAOPOA010000599         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_79951_length_1650_cov_3.175549, whole genome shotgun sequence.
ACCESSION   DAOPOA010000599 DAOPOA010000000
VERSION     DAOPOA010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79951_length_1650_cov_3.175549"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1650)
                     /locus_tag="PKJ17_10865"
     CDS             complement(<1..>1650)
                     /locus_tag="PKJ17_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOD76522.1"
                     /translation="IPGWDCHGLPIEHQVEKNVKQKKLELSKLETRKECRAYARQFID
                     IQREEFKRLGGIGDWDHPYITMDFDYQATIIEEAAKFFIRGEVYRKKKPVYWCLSCET
                     ALAEAEIEYDTKRSSSIYVKFPYTGEREPVFAGYPDKPVHMLIWTTTPWTLPANLAIA
                     VHPDFTYVTVETDKEVYIVLKDLLEDVMRRAGITGYRVIGEIDPETLRGLAFRHPFID
                     RQSVIIFADYVAADTGTGAVHTAPGHGEEDYETGLKYGLDIYSPVNEKGVFIDEVEFF
                     KGMNVWEANPKVIEKLKELGLLLHTEEIEHSYPHCWRCKKPVIFRATEQWFVSMDSHG
                     LRQKALAEVDRTRWIPSWGRDRIYNMLSVRPDWCISRQRTWGIPITIFYCEKCREPFW
                     SEASFARVVEAVRQSGADVWFEKDAAHFLPDGTKCGHCGNTAFSKEQDILDVWFDSGV
                     SWAAVCKKRRELEYPADLYLEGGDVALEKVTTPDSRIETTYEPQGRRYVMRWPGRGTY
                     KTTVAFAARPRLVESGAWREVEFDLPSSRLRDLEVVCDRAD"
BASE COUNT          280 a          500 c          514 g          356 t
ORIGIN      
        1 agtcggcgcg gtcgcaaacg acctccaggt cccgcagccg cgaggacgga aggtcgaact
       61 ccacctcgcg ccaggcgccc gactccacca atcgtggccg ggcggcgaag gccacggtcg
      121 tcttgtacgt tcccctgccc ggccagcgca tgacgtagcg gcggccctgc ggttcgtagg
      181 tcgtctcgat gcgtgaatcc ggcgttgtca ccttctccag cgccacgtcg cccccctcga
      241 gatagaggtc ggcagggtat tccaactccc gcctcttctt gcagacggcg gcccaactca
      301 cacccgaatc gaaccatacg tcgaggatgt cctgttcctt ggaaaaggcc gtgttgccgc
      361 aatggccgca cttcgtgccg tcgggcagaa agtgggcggc gtccttttcg aaccagacgt
      421 ccgcgcccga ttgccgcacg gcctcgacga cacgcgcgaa ggacgcttcg ctccagaagg
      481 gttcgcgaca tttttcgcag tagaagatgg tgatggggat gccccaggtg cgctgccggg
      541 agatgcacca gtcggggcgg acggaaagca tgttgtagat cctgtcgcgg ccccaggagg
      601 ggatccaccg cgtcctgtcg acctcggcca gggccttctg tctcagtccg tggctgtcca
      661 tggagacgaa ccactgctct gtcgcccgga atatgacggg cttcttgcag cgccagcaat
      721 gggggtagga gtgctcgatt tcttcggtgt ggaggaggag ccccagttct ttgagcttct
      781 cgatgacctt cggattggcc tcccagacgt tcatcccctt gaagaattcc acctcgtcta
      841 tgaagacgcc cttttcattg acgggggaat agatgtcgag gccatacttg agtcccgtct
      901 cgtagtcctc ttcgccgtgg cccggtgccg tgtggacagc gcccgttccc gtatcggcgg
      961 ctacgtagtc cgcgaagatg atgacggact gccggtcgat gaagggatgc ctgaaggcga
     1021 gcccccgaag cgtctcaggg tctatctccc cgataacgcg gtatcccgtt atccccgcgc
     1081 gccgcatgac gtcctcgagg aggtctttga ggacgatgta gacctctttg tcggtctcaa
     1141 cggtgacgta ggtgaaatct ggatggacgg cgatggcaag gttcgcggga agcgtccagg
     1201 gtgtggtcgt ccagatcagc atgtggaccg gtttgtcggg atagcccgcg aagaccggtt
     1261 ctctctcgcc cgtgtagggg aacttgacat atatggaact ggacctcttg gtgtcgtatt
     1321 cgatctccgc ttcggcgagg gccgtctcgc agctgaggca ccagtagacg ggcttcttct
     1381 tccggtagac ctcgccgcgg atgaagaatt tcgccgcctc ttcgatgatc gtcgcctggt
     1441 agtcgaaatc catggtgata tagggatggt cccagtcgcc gatgccgccg aggcgcttga
     1501 actcctcgcg ctggatgtct atgaactgcc gcgcataggc ccggcattcc ttccgggttt
     1561 ccagcttgga gagctcgagc ttcttctgct tgacgttctt ctccacctgg tgttctatgg
     1621 gcaggccgtg gcagtcccat ccggggatat
//
LOCUS       DAOPOA010000600         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_80401_length_1643_cov_3.974181, whole genome shotgun sequence.
ACCESSION   DAOPOA010000600 DAOPOA010000000
VERSION     DAOPOA010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80401_length_1643_cov_3.974181"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="PKJ17_10870"
     CDS             <1..1135
                     /locus_tag="PKJ17_10870"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOD76523.1"
                     /translation="EAAEREIFGKPIRSIRDVEFRNVFRLFLGWEFNHFVKNWEEIFA
                     YHMAFYKARTGREQLRQLYSDMSGREAEYLEEVYDRAEKIPSEKIHRRSVNVEAEGGS
                     GQRYLVYTTFFREGMFFLYVRADRTRKSIAEFLSDRETVVRDLLQQRLPTLVSFSVLV
                     ADLQNSVRICAELPPEEYFELINSMWRILEESFRRFNGIYGKRIGDGVVYYFLKKQDP
                     NYLMNSINCALAIRARMADFSNEWKTRKRWLNDLYLNIGINEGQEYFSTTTPSSTNIE
                     FTALGDTINYASRLSNLARFGSILTTKNVMNHLNDDDRRAVRYGIRKRLEDRELFVEN
                     VFSRVDDLLKDNDTWRQRFADIATLPVTEITEGRAQAPKQDPI"
     gene            1251..>1643
                     /locus_tag="PKJ17_10875"
     CDS             1251..>1643
                     /locus_tag="PKJ17_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD76524.1"
                     /translation="MEIITARDLIKDYDGLRAVDRVSFTIIKGECFGFLGPNGAGKTT
                     IMRIIYCFLPPTGGEVRVLGHDVTREPSLIKSRMGVMPQDDSLDLDLSVIQNLVVYGR
                     YFDMPKRKTLPLAEKLLASVGLADKMKAN"
BASE COUNT          374 a          444 c          487 g          338 t
ORIGIN      
        1 cgaagccgcg gagcgcgaga tattcgggaa gccgatcaga tcgatacggg atgtcgagtt
       61 cagaaacgtc ttccggcttt tcctgggttg ggagttcaac cattttgtga agaactggga
      121 ggagatcttc gcttaccaca tggccttcta taaggccagg accggcaggg agcagctccg
      181 tcaactttac tcggacatgt ccggcagaga agcggaatat ctggaagagg tgtatgaccg
      241 ggccgaaaag ataccatcgg agaagatcca tcgccgcagt gtcaatgtag aggctgaagg
      301 agggtccggg cagaggtatc tcgtgtacac gacctttttc cgtgaaggga tgttcttcct
      361 ctatgtccgg gcggacagga cccggaagag catcgccgag tttctctccg accgtgagac
      421 cgtggtgagg gacctgttgc agcagcgcct gcccacgctg gtttccttca gcgtgcttgt
      481 cgccgatctg cagaactccg tccgcatctg cgcggagttg ccgcccgagg aatatttcga
      541 gctgataaac agcatgtgga ggattctgga ggaatccttc aggcgtttca acgggatata
      601 cggcaagagg atcggcgacg gcgtggtcta ctatttcctg aagaagcagg accccaatta
      661 cctgatgaac tcgatcaact gcgccctggc gatacgggcg aggatggcgg atttttccaa
      721 cgagtggaag acccgcaaga gatggctcaa cgatctgtac ctcaacatag gcataaacga
      781 gggacaggag tacttcagca ccacgacgcc gtcctcgacg aacatcgaat tcacggccct
      841 tggcgatacg atcaactacg cgtcgagact gtcgaacctt gcgcggttcg gctcgatcct
      901 gaccacgaag aacgtcatga accacctgaa tgacgacgac aggagggccg tccgttatgg
      961 catcagaaaa agactggagg atcgggaact gtttgtcgag aatgtctttt cccgggtcga
     1021 cgatctcctc aaggataacg atacgtggcg ccagcggttt gccgacatcg cgaccttgcc
     1081 ggtaacggag atcacggaag gaagagcaca ggcaccgaaa caggatccca tttgaccggt
     1141 cacgcgtagc ccgcgagacc cgcgcacgcg cctgatgcgg cgggtgaccg gacggtacgc
     1201 cgctcccctg tttctttccg cccttgtttt ctgctatcat tacgtcattc atggagatca
     1261 taaccgccag ggaccttatc aaggattatg acgggctgag ggccgtcgac agggtcagct
     1321 tcaccatcat caaaggggag tgcttcggtt ttctcggacc gaacggggcc ggcaagacga
     1381 cgataatgcg catcatctac tgctttctcc cgccgacggg cggagaggtg agggtcctcg
     1441 ggcatgacgt cacgagggag cccagtctca tcaagtcgcg catgggtgtc atgcctcagg
     1501 acgacagtct cgacctcgac ctttccgtta tccagaacct tgttgtctac ggcaggtatt
     1561 ttgatatgcc gaagaggaag acgctgcccc tggcagagaa gcttctcgcc tccgtcggtc
     1621 tcgcggacaa gatgaaggcc aat
//
LOCUS       DAOPOA010000601         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_80616_length_1640_cov_4.543849, whole genome shotgun sequence.
ACCESSION   DAOPOA010000601 DAOPOA010000000
VERSION     DAOPOA010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80616_length_1640_cov_4.543849"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..>1640)
                     /gene="recJ"
                     /locus_tag="PKJ17_10880"
     CDS             complement(179..>1640)
                     /gene="recJ"
                     /locus_tag="PKJ17_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOD76525.1"
                     /translation="LVEAVDRGERVCIWGDYDADGISCLALMFNFLNHLGLVPVVHIP
                     TREEGYGLHIDKVEDLAKQGVDLIVSLDCGASNVEEIAHARSLGVDVIVVDHHEMGDE
                     VPPAVAVINPKRRGSRFPTRDLAACGVTFFFLLGLRRVMHGKGLIPAHINLKKELDLV
                     TVGTVGDMVPLLGDNRILVRHGMEVMNAKPRAWLKSMLDSRVISKGLINEFALGFIIV
                     PRINAAGRVASPHPALDFLVAKETGSASQLLAHLQKLNNVRQQREKKIHEEIVASIGR
                     DGLSGRKTIVVFDEKWHAGVLGIVAQKLTEQFERPAIVITRVNGIWKGSGRGGDGMDL
                     FETIGSVSHLLLKYGGHKYACGISLEEENLPLFAEAFEKAVEGNITARRRVVHVDTAA
                     GFEELTAEVMERLEDLGPFGIGNPRPNLALEAAGVFAAPNGRTRITDGDNRTWYGFYA
                     PKGSLQREGGARIIASPVVKMEMGERFIHLNIKEVE"
BASE COUNT          323 a          501 c          449 g          367 t
ORIGIN      
        1 agaaaagccg gacgccgttt ttcctgtctt tgcctaacac gtaaaacgta aaacctaaaa
       61 cggcttttct accgttttaa gttttaagtg ttaagtttta agttaaaagg agaaaagccg
      121 gacgccgttt ttcctgtctt tgcctaacac gtaaaacgta aaacccaaaa cggcttttct
      181 attctacttc ttttatattg aggtgtatga agcgttctcc catttccatt ttgacgacgg
      241 gggaggcgat tatccttgcg ccgccttccc tctggaggga accttttggt gcgtagaagc
      301 cgtaccaggt cctgttgtcc ccatcggtga tcctggtgcg tccgttcggc gcggcgaaga
      361 cgcccgcagc ctcaagggca aggttcgggc ggggattgcc gatgccgaag gggccgaggt
      421 cttccagtct ttccatcacc tccgccgtaa gctcctcgaa tccggcggcg gtgtccacgt
      481 gcaccacacg ccttcgcgct gtgatattcc cctccaccgc cttttcgaac gcctcggcaa
      541 agagcggcag gttctcctcc tccagtgaga tgccgcatgc atacttgtga ccgccgtatt
      601 tcagaaggag atgcgagacg gagcctatgg tctcaaaaag gtccatgccg tcgcctcccc
      661 ttccggaacc cttccatatc ccgttgaccc tggtaatgac gatggctggc ctttcgaact
      721 gctcggtcag cttttgggct acaatgccca ggacgcccgc gtgccacttt tcatcgaaga
      781 cgacgatcgt cttcctgccc gaaagaccgt cgcggcctat ggaagcgacg atctcctcgt
      841 gtatcttctt ctcccgctgc tgcctcacgt tgttcagctt ctgcaggtgc gcgagcagtt
      901 gagacgccga cccggtctcc tttgccacca ggaaatcgag ggccggatgg gggctcgcaa
      961 ccctgccggc ggcattgatc cggggaacga tgatgaaacc aagggcaaat tcgttgatga
     1021 gccccttcga tatgaccctg ctgtcgagca tcgacttgag ccatgcccgc ggcttcgcat
     1081 tcatgacctc catgccgtgg cgcacgagta tcctgttgtc ccccaaaagc ggcaccatgt
     1141 cccccacggt ccccacggtc acgaggtcga gctctttctt gaggttgatg tgcgccggaa
     1201 tgagaccttt tccgtgcatc acgcggcgca gcccgaggag aaagaagaag gtgacgccgc
     1261 acgcggcgag gtccctcgtg ggaaaccggg aaccgcggcg tttcgggttt atgactgcca
     1321 ccgccggggg aacctcgtca cccatctcgt gatggtcaac gacgatgacg tccacgccga
     1381 ggctcctggc atgggctatc tcctcgacgt tggaggcacc gcagtcgagg gaaacgataa
     1441 ggtccacgcc ctgtttagcg aggtcctcca ccttgtcgat gtgaagcccg tatccctcct
     1501 cccgggtggg tatgtggacg acgggcacga gtcccagatg gttcaggaaa ttgaacatca
     1561 aggcgaggca ggaaatgccg tccgcatcgt agtcgcccca tatgcagacg cgctcccccc
     1621 tgtcgaccgc ctcgacaagc
//
LOCUS       DAOPOA010000602         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_80696_length_1639_cov_3.025884, whole genome shotgun sequence.
ACCESSION   DAOPOA010000602 DAOPOA010000000
VERSION     DAOPOA010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80696_length_1639_cov_3.025884"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PKJ17_10885"
     CDS             complement(<1..829)
                     /locus_tag="PKJ17_10885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD76526.1"
                     /translation="MTLEEKIYDGKRIDKEEALGLFSWNIPELGMAAEFRRSLVSPGD
                     TVGFILDRIINFTNVCEASCDFCAFHALAGRVAPYELTLDEILAKVEELASIGGTQVM
                     LQGGLNPRHTIDTYLDMIETVKHRFPDIYLHSFSPAEIIHVARRSKISLDEAVEGLKA
                     AGLDSVPGASDILVDRIRSRVSPRKHTTAQWLEVIDTLSRHGLKSSATMTYGMGETLE
                     ERIEHLEMVRTAQDRTGILRAFIPWSFSPGHTRMDNVPSATGIDYLKIVAVSRIFLDN
                     "
     gene            complement(826..>1639)
                     /locus_tag="PKJ17_10890"
     CDS             complement(826..>1639)
                     /locus_tag="PKJ17_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOD76527.1"
                     /translation="NEPLPDVRIHPGYPSVLNRMMGTGELDLSPISAASYANLEESVV
                     LLPDFCISSNGYVQSVILASRLPIEELDGKTVGLSRASHTSEVLLKVLMRKYYAIEPV
                     YVPADPNPWLTGQGIDAALIIGNDAMLQKPAEHVYDLGSLWLEKTGHPVVFAVFAVRE
                     SAVSNSLKAVEAIVHSYTRSLFCLESERQRLIQKARGKYPLIKSDIDAYYRVLQYSFT
                     DIRKEALVFYLLSAGELGLIRKVGGLKLLDLPQRATEDGMHISLKNNGALRK"
BASE COUNT          338 a          440 c          492 g          369 t
ORIGIN      
        1 tgttgtccag gaagatgcgt gaaacggcga cgatcttcaa atagtctatc cctgttgccg
       61 acggcacatt gtccatgcgt gtatggcccg gcgaaaacga ccaggggatg aaggcccgca
      121 gaataccggt gcggtcctgt gcggtacgga ccatttcgag atgctcgata cgctcctcca
      181 gcgtctcccc catcccgtat gtcatggtag cgcttgactt gagaccgtga cgcgacaggg
      241 tatcgatgac ctccagccat tgcgcagtgg tatgtttcct cgggcttacc ctgctccgta
      301 tccggtcgac gagaatgtcc gacgccccgg ggaccgagtc gaggccggcg gctttcaggc
      361 cctcgactgc ctcatcgagg gagatctttg accggcgtgc cacgtggatg atctctgctg
      421 gggagaacga atgcaggtag atgtcgggga agcggtgttt gaccgtttcg atcatgtcaa
      481 gataggtgtc aatggtgtgc cggggattga gaccgccctg aagcattacc tgtgttcccc
      541 cgatggatgc gagctcttcg actttggcca ggatctcatc cagcgtgagc tcgtaaggcg
      601 caacgcgccc tgcaagtgcg tggaaggcgc agaagtcgca cgaggcttca cacacattcg
      661 tgaaattgat tatgcggtcc aggatgaagc ccaccgtgtc ccccggggaa accagagacc
      721 ttcggaactc cgcggccatg ccaagttcgg gtatattcca ggaaaaaagg ccgagagctt
      781 cttccttgtc gatccttttc ccgtcgtata tcttttcttc cagggtcatt ttctcagtgc
      841 tccattgttc ttcaaagata tgtgcatacc gtcttccgtc gcccgttgcg gaaggtccag
      901 taatttcagc cctcccacct tcctgatcag cccgagttcg ccggcgctta gcagatagaa
      961 gacaagcgcc tctttccgaa tgtcggtgaa ggagtactgg agcacgcggt agtatgcgtc
     1021 gatatcgctt ttgatcagcg gatactttcc cctggctttt tggatgagcc tttggcgttc
     1081 cgactccagg cagaaaaggg atcgagtgta ggaatgcacg atcgcctcga cggccttgag
     1141 ggagttgctg accgcggatt cccggaccgc aaagacggca aagaccacgg ggtgaccggt
     1201 cttctcgagc cacagggagc caaggtcata gacgtgttcg gctggctttt gcagcatcgc
     1261 gtcgttcccg atgatgaggg cggcatcaat tccctggccg gtcaaccaag ggttcgggtc
     1321 ggcggggaca tagacgggct caatggcgta gtatttccgc atcaggactt ttaagagcac
     1381 ctcggaagta tgcgatgcac gggacaaacc gacggttttc ccatcgagtt cttcgatagg
     1441 gagccggctg gcgagaatga cagactgtac ataaccattg gaactgatgc agaaatcggg
     1501 aagaaggacg acgctctctt cgaggtttgc gtatgaggcg gcggagatcg ggctcaagtc
     1561 gagctcccct gtgcccatca tgcgattcag gacgctggga taaccggggt ggatacggac
     1621 atccggcagg ggttcgttt
//
LOCUS       DAOPOA010000603         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_81325_length_1630_cov_3.093333, whole genome shotgun sequence.
ACCESSION   DAOPOA010000603 DAOPOA010000000
VERSION     DAOPOA010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81325_length_1630_cov_3.093333"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKJ17_10895"
     CDS             complement(<1..312)
                     /locus_tag="PKJ17_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76528.1"
                     /translation="MVRKTTALSGNTPEVLDSSLSLLEELFVGDATPPEIAGKLLSHI
                     RRRTGVWDPYATLKYREYRDALAAVGGLRGGECLEDLLSLSALGNSTDYFTGGTFMNE
                     LP"
     gene            602..1543
                     /locus_tag="PKJ17_10900"
     CDS             602..1543
                     /locus_tag="PKJ17_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76529.1"
                     /translation="MADKRRTSVYVDYHILDLISMTRVSDEALLSEWKAGRSIWDRRG
                     QEMVSLVTSVDEMELDFVIQMNRGGLCVTDTFQITDNIDNFERWEGADHADTDHWRAI
                     VELYDQLDVISGHDDLVGEHAYPHYCEQLARVLKEEPAGDTGRSASFDEETAILRDCA
                     AALHDVYDMQLWADLKHIQYGLNWKVLRSVLPRYSRSAVLNGEDAGLNKNLLGLLNRL
                     VNIGKKSCPRLPMQDRHIDFVLDIVRKKYCQEDIDRSISHIAHCLRGGIDCYLTTDGE
                     LAERFAERKQNLQLALGTSIHLELLRPTELEKKLGAA"
BASE COUNT          368 a          456 c          483 g          323 t
ORIGIN      
        1 ggggagttca ttcatgaatg tcccgccggt gaagtagtcc gtggagttgc cgagggcgga
       61 cagactgagc aggtcttcca aacactcgcc gccacgaagt cccccgacgg cggccagagc
      121 gtctctgtac tcgcgatatt tgagggtggc gtagggatcc cagacgccgg tccttcgcct
      181 gatgtgcgac agcagctttc cggctatctc cggcggggtc gcgtccccga cgaaaagctc
      241 ctcgagaagc gacagtgacg aatcgaggac ctccggggta ttcccggaaa gggccgtcgt
      301 cttccggacc aatccttcca gacatgcgcg acagggctcc tgaactttca tgacaggcaa
      361 taggatataa tcaaatgata tgcatttcaa cgtcaaagga gcgccgtggc gctcccgaaa
      421 taaacgcttt cattcttttc ctcatttgtt ataatggggg caggggctta agggtggttc
      481 caggccatac ggattgacgc tcatcctcac gttcgtcgct caaattctct ttctcgtcgc
      541 ctgacatgct cttccggcac gatctatgtt tcacggggat tccccggaca aagggtgaca
      601 gatggcagac aagcggcgca cgagcgtcta cgtggattat cacattctcg acctcatctc
      661 gatgacccgg gtgagcgatg aggccctcct atccgagtgg aaagcgggca gatcgatctg
      721 ggaccgtcgc gggcaggaga tggtatctct tgtcacctcc gtggatgaga tggagctcga
      781 tttcgttatc cagatgaacc gcggcggcct ctgcgtcacc gatacctttc agatcaccga
      841 caatatcgac aacttcgaac gctgggaagg cgcggaccac gcagataccg atcactggag
      901 ggccatcgtg gaactctatg accagctcga cgtcataagc gggcatgacg atcttgtcgg
      961 ggaacatgcc tatccccact attgcgagca gctggcccgc gtcctgaagg aggagcctgc
     1021 cggagacacg gggaggtcgg cctcgttcga tgaggagacg gcgatactca gggactgcgc
     1081 ggcggcgctg catgacgtct atgacatgca gctctgggcg gacctcaagc acatccaata
     1141 cggcctcaac tggaaggtcc tccgatccgt cctgccgaga tattcgcgca gcgccgtgct
     1201 caacggcgag gacgcggggc tgaacaagaa ccttcttggg cttctcaacc ggctcgtcaa
     1261 cattgggaag aagagttgcc cccggctgcc catgcaggac cggcacatcg acttcgttct
     1321 cgatatcgtg aggaagaagt actgccagga ggatatcgac cgcagcatct cgcacatagc
     1381 ccactgcctg cggggcggta tcgactgcta ccttactaca gacggtgaac tggcggagag
     1441 attcgccgaa aggaaacaga acctccaact cgccctggga acatccatcc acctggaact
     1501 cctgcgcccg acggaactgg agaaaaagct aggggcggct tgaattgctt gagtagcttg
     1561 agtcgcttga gagttaaatg aaaaggaaga cggagagcga tttggagcag gcagcaacgg
     1621 tggggataaa
//
LOCUS       DAOPOA010000604         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_81588_length_1626_cov_4.651814, whole genome shotgun sequence.
ACCESSION   DAOPOA010000604 DAOPOA010000000
VERSION     DAOPOA010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81588_length_1626_cov_4.651814"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKJ17_10905"
     CDS             <1..443
                     /locus_tag="PKJ17_10905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD76530.1"
                     /translation="HVHRNLVPGIPIARGNASILHQVFMNIVSNAIEAMEDGGILTVS
                     SRFLPEQRKVVVDIQDTGPGILREHVDGVFDPFFTTKAAEDGTGLGLAISYSMVRKMG
                     GDIKVISSRVEREKFPGETGTIFSIELPALEDNGLHKQKEGDQN"
     gene            440..>1626
                     /locus_tag="PKJ17_10910"
     CDS             440..>1626
                     /locus_tag="PKJ17_10910"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOD76531.1"
                     /translation="MNPANVLIVEDDTKLRNALKEIMTREGYTVDVTESGDIAVSMIK
                     DTVYDLVITDLKLPGIDGMDVLRSVQKLARDTSVIIITAYATVDTAVEAMKEGAEDYI
                     AKPFNLDEIRLIVRKVLDKKALVDDNRFLRSQLKKKYSFGNIVGSSEAMIEVYKMIEK
                     VKDSKATVLILGETGTGKELVARAIHFNSIRSEKPFLPVNCAALAETLLESELFGYAK
                     GAFTGATRDKQGVFEVAHGGTVFLDEIGDISLRLQQILLRVLENGEIQPVGSTQRRKV
                     DVRVVAATNKDLEEMIKTGAFRADLYYRLNVITLDLPSLRERKEDIATLADHFLKKYA
                     EENKRPIYRISREALNLLDRYEWPGNVRELENVIERATLLETSKEITPESLPESIRTA
                     PEP"
BASE COUNT          421 a          427 c          469 g          309 t
ORIGIN      
        1 ttcacgtcca caggaacctc gttcccggca tcccgattgc aagaggtaat gcaagtattt
       61 tgcaccaggt gtttatgaat attgtctcaa atgcgatcga agcgatggaa gatggcggta
      121 ttttaacggt ttcctcccga ttcttgcctg aacagcgcaa ggttgtcgtc gacatacagg
      181 acacaggccc gggcatatta agagagcatg tagatggtgt atttgacccg ttcttcacta
      241 ccaaggccgc ggaggacggg accggcctcg ggctcgccat aagctattca atggtaagga
      301 agatgggcgg cgatatcaaa gtgatcagtt cgcgcgttga aagagagaag ttccccggcg
      361 agacggggac catattcagc atagagcttc cggccctcga agataacggt ctgcataagc
      421 aaaaggaagg tgaccagaat tgaaccccgc aaatgtgctc attgtcgaag acgatacgaa
      481 gttaaggaac gcccttaagg agataatgac ccgtgagggc tacacggtcg atgtcacgga
      541 atctggcgat atcgccgtgt cgatgatcaa ggataccgtc tatgaccttg taataacgga
      601 cctaaagctg ccgggcatcg atggcatgga cgtccttcgg tcggtgcaga agctggcgcg
      661 ggacacgagc gtcatcatca ttaccgccta tgcaacggtg gatacggctg tcgaggcgat
      721 gaaggagggg gccgaggact acattgcgaa acccttcaac cttgacgaga tcaggcttat
      781 cgtcagaaag gtcctcgaca agaaggccct cgtcgatgac aaccgctttt tacggagcca
      841 gctgaagaag aaatacagct tcggcaacat cgtgggcagc agcgaggcca tgatcgaggt
      901 ctataagatg atcgaaaagg tgaaggactc gaaggcgacc gtccttatcc tcggtgagac
      961 cggcacaggc aaggaactag tggcgcgtgc catccacttc aacagcatcc ggtcagagaa
     1021 gccctttctg cccgtcaact gcgcggccct cgcggaaacg ctcctcgaaa gcgaactctt
     1081 cggctacgcg aagggcgcat tcacgggagc gacacgggat aagcagggcg tgttcgaggt
     1141 ggcccacgga ggcacggtat tcctagacga gatcggcgat atcagcctcc gtttgcagca
     1201 gatattgcta cgcgtgctcg agaacggcga gatccagccc gtgggttcca cacagcgcag
     1261 gaaggtcgac gtccgcgtcg tcgccgcaac gaacaaggat ctggaagaga tgataaagac
     1321 aggcgcgttc agggcggacc tctactatcg acttaacgtc atcacccttg acctgccctc
     1381 cttgagggaa aggaaagagg acatagccac ccttgccgac cacttcctca agaagtatgc
     1441 ggaggagaac aagaggccta tttatcgcat atccagggaa gcgctcaatt tgctcgaccg
     1501 ctatgaatgg ccgggcaacg tccgggaact cgagaacgtg atagaacggg cgacccttct
     1561 cgaaacatcg aaggaaatca cccccgagag cctgcccgag agcatacgca ctgccccgga
     1621 gccttt
//
LOCUS       DAOPOA010000605         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_81692_length_1625_cov_2.817197, whole genome shotgun sequence.
ACCESSION   DAOPOA010000605 DAOPOA010000000
VERSION     DAOPOA010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81692_length_1625_cov_2.817197"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKJ17_10915"
     CDS             complement(<1..605)
                     /locus_tag="PKJ17_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOD76532.1"
                     /translation="MSKKTFVRDITGSQKEVNDLFVVTKKGTYTSRENKKYMMIGLKD
                     ATGSIDGRIWEAVDELEGLFERGDIVSVQSKVKDYQNKLQLHITNIRKLDADLAVDDI
                     DHFYPAGDRDRESLEKEYGEIVRAMGNPWLTKLLAAFHEDVQLRKRFFSYPASTTVHH
                     VYVGGLLDHSLCMARMGMAAVSFIGGDRDIVIAGSILHDIG"
     gene            complement(602..1567)
                     /gene="thiL"
                     /locus_tag="PKJ17_10920"
     CDS             complement(602..1567)
                     /gene="thiL"
                     /locus_tag="PKJ17_10920"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOD76533.1"
                     /translation="MEISENNFIKGIRKFSRKSNSVIRGIGDDGAVAELGAGQYVFVQ
                     DAMAEHVHFEFSFMDPYYVGKKALYSNISDVLAMGAQPVFYLVTVGIPAGISSGQMER
                     MYRGMNRVAREFGVTLLGGDTVATAKDLFIDISVTGKLAGARYFGRDKARRGDLIAVT
                     GKLGEAALGLKVLKEGGDATGMKNCVRRFLSPAPPYGLWKEIVKDDITDVMMDVSDGL
                     ILDLSRMMDESRKQARIHWEKVPMPRPLRQRGLEELAFGGGEDYQLLFTFPREKLPVV
                     AAMIDKGRAVTIIGEVVSGRGVRVYRHGSEVTSPPGGYDHFGGRL"
BASE COUNT          323 a          506 c          426 g          370 t
ORIGIN      
        1 tttccgatat cgtggaggat ggaaccggcg atcactatgt cccggtcccc tccgatgaag
       61 gatacggcgg ccatgcccat ccgtgccatg cagagggagt gatcgagcag acccccgaca
      121 tacacgtggt gtacggtggt cgaggcggga taggaaaaga acctctttct cagttgcacg
      181 tcttcatgga aggcagcgag gagcttcgtg agccagggat tccccattgc cctgacgatc
      241 tcaccgtact ccttctccag ggactccctg tccctgtcgc ccgcgggata gaagtggtca
      301 atgtcatcca cagccagatc ggcatcgagc ttcctgatgt tggtgatatg cagctgcagc
      361 ttgttctggt agtccttgac ctttgattga acggagacga tgtcgcccct ctcgaagagg
      421 ccttccagtt cgtcgacagc ttcccagatc ctgccgtcga tggaacccgt cgcgtccttg
      481 aggcctatca tcatgtattt cttgttctcc cgtgaggtgt aggtaccctt cttcgtgacg
      541 acgaagagat cgttgacctc cttctgacta cctgttatgt cccgcacaaa cgtcttcttg
      601 ctcacaacct gcccccaaaa tgatcgtagc cccccggggg gctcgtgact tccgaaccgt
      661 gcctgtagac cctgactccc ctgcccgaca cgacctctcc gatgatggtg acggccctgc
      721 ctttgtcgat cattgccgct acgacgggaa gtttctcccg ggggaacgtg aagagaagct
      781 ggtaatcctc cccgccgccg aaggcgagct cttcgagccc cctctgcctg agtgggcggg
      841 gcatgggaac cttttcccaa tggatgcggg cctgtttcct gctttcgtcc atcatcctgg
      901 aaaggtccag gatgagtcca tcgctcacat ccatcatcac atcagttata tcatctttca
      961 cgatctcctt ccacaagccg taaggcggag ccgggctaag gaaacgccgg acgcagttct
     1021 tcatacccgt cgcgtccccc ccttccttga ggactttgag gcccagtgct gcctctccga
     1081 gctttcccgt gacggcgata agatctcccc ttctcgcctt gtccctgccg aagtagcgcg
     1141 ccccggccag cttcccggtc acggagatat cgatgaagag gtccttcgcc gtggcgaccg
     1201 tgtctccccc gaggagggtg acgccgaatt cccgggcgac cctgttcatt ccgcggtaca
     1261 tcctttccat ttgccccgag gagatcccgg cgggtattcc cacggtcacg agatagaata
     1321 ccggctgcgc ccccattgcc aggacgtccg atatattcga gtaaagggcc ttcttgccca
     1381 cgtagtaagg gtccatgaaa gagaactcga agtggacatg ctccgccatg gcatcctgca
     1441 cgaagacgta ctggccggcg ccaagctccg cgacggcacc gtcgtcaccg ataccccgta
     1501 tgacactatt actttttcgg gagaactttc gaatgccctt aatgaaatta ttttccgata
     1561 tctccatgat gaaggaacat gataacccaa accgaaaggc ggttgcaacc gcaaggggtt
     1621 aaagg
//
LOCUS       DAOPOA010000606         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_81693_length_1625_cov_2.792357, whole genome shotgun sequence.
ACCESSION   DAOPOA010000606 DAOPOA010000000
VERSION     DAOPOA010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81693_length_1625_cov_2.792357"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..695
                     /locus_tag="PKJ17_10925"
     CDS             108..695
                     /locus_tag="PKJ17_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOD76534.1"
                     /translation="MAFRYDIRLSGSGGQGIILMGIILAEAIGIYDGMFVAQTQSYGP
                     EARGGSSKAEVVVSDGEIDYPKALQLDLLVAMNQKSCDDYYMDLKPDGILVVDSTLVR
                     QVPLSRAYQIPFTRIAREELKREMVANIVALGALARLTPIVSTRAIESAVLGRVPKGT
                     EKLNRDALQAGMRAARKTKKVRFPEKISELVDEDS"
     gene            682..>1625
                     /gene="sucC"
                     /locus_tag="PKJ17_10930"
     CDS             682..>1625
                     /gene="sucC"
                     /locus_tag="PKJ17_10930"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOD76535.1"
                     /translation="MKIHEFQAKKLLSEYGVPIPRGQIARTDEEALNVARQLGEGPFV
                     LKAQVHAGGRGKAGGIRVVNNIDDVGRAAWEMLGKTLVTAQTDEEGKPVRTLLVEEAV
                     NVGKEVYIGIVIDRDKACPVILASPEGGVQIERLARERPDRVITEVVDPSVGFRPFQA
                     NRIFYGLGLDESLARPVMASVIGLYRLLVEKDLSLAEINPLVVTDEGGIIALDAKVVA
                     DDNALFRHPDIAGLRDVRQEDPLEVEAGKHNLNYIKLKGNVGCMVNGAGLAMATIDLI
                     KLAGAEPANFLDVGGGATSRMVKEAMRILVFDKDVKMI"
BASE COUNT          370 a          433 c          534 g          288 t
ORIGIN      
        1 gaaggacaag ttcaggatag gtgtccttat cgacagggat ctgcccccgt accaggatga
       61 gtacgaaaag gtacggaggt ggtcgaaggg cgcggagagg aaatagcatg gcctttcggt
      121 acgatatcag gctcagcggc tccggagggc aggggatcat tctcatgggc atcatcctcg
      181 ccgaggccat aggcatctac gacggcatgt tcgtggcgca gacgcagagc tacggccccg
      241 aggcgcgcgg cggttcgagc aaggccgagg tcgttgtcag cgacggcgag atcgattacc
      301 ccaaggccct ccaactggac ctcctcgtgg ccatgaacca gaagtcctgc gatgattact
      361 acatggacct caaacctgac gggatcctcg tcgttgattc gaccctggtc aggcaggtac
      421 ccctgtcgag ggcgtaccag atacctttta cacgcatagc gcgggaagaa ctgaaaagag
      481 agatggtcgc caatatcgtc gctctggggg ccctggcgcg gcttaccccc atagtctcca
      541 cccgggccat tgaaagcgcc gtcctcggcc gcgtccccaa ggggacggag aagctcaaca
      601 gggatgctct ccaggcgggg atgagggcgg cccggaagac gaaaaaggtg cggtttcccg
      661 agaagatctc ggagcttgtc gatgaagatt catgagtttc aggcgaaaaa gcttctttcg
      721 gagtacggtg ttcccattcc gcgggggcag atagcgagaa cagacgaaga ggcgttgaat
      781 gtcgccaggc agctcggcga ggggccgttt gtcctaaaag cgcaggtcca cgcgggcgga
      841 cggggaaagg ccgggggcat cagggttgtg aacaacattg acgacgtcgg ccgcgcggcg
      901 tgggaaatgc tcggaaagac cctggttacc gctcagacgg atgaggaggg aaaaccggtc
      961 cggacactcc tcgtcgaaga ggcggtgaac gtcggcaagg aggtctatat cggcatcgtc
     1021 atcgacaggg ataaagcctg ccccgtgata ctggcgagtc cggaaggggg tgtgcagatc
     1081 gaaagacttg cccgggagcg accggaccgg gtgatcaccg aggtcgtcga cccctccgtg
     1141 ggatttcgac ccttccaggc gaacaggata ttctacgggc ttggtctcga cgaatccctt
     1201 gcccggccgg tgatggcctc cgtgatagga ctgtacagac tccttgtcga gaaggacctt
     1261 tccctcgccg agatcaatcc cctcgttgtg acggatgaag gaggcataat tgccctcgac
     1321 gcaaaggtcg tcgccgatga caacgcccta ttccggcatc ccgacatcgc gggcctgagg
     1381 gacgtaagac aggaagatcc gctcgaggtg gaagcgggga aacataacct caactatata
     1441 aagctcaagg ggaatgtggg ctgcatggta aacggggcag gccttgccat ggcaacgatt
     1501 gacctcatca agctggccgg cgcggaaccc gccaacttcc tggatgtcgg cggaggggcg
     1561 acgtcgcgaa tggtaaagga ggccatgagg atcctcgttt tcgacaagga tgtgaagatg
     1621 atatt
//
LOCUS       DAOPOA010000607         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_81852_length_1623_cov_2.046556, whole genome shotgun sequence.
ACCESSION   DAOPOA010000607 DAOPOA010000000
VERSION     DAOPOA010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81852_length_1623_cov_2.046556"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PKJ17_10935"
     CDS             <1..316
                     /locus_tag="PKJ17_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD76536.1"
                     /translation="EGGETLEIGNMVWDVIHIPGHSMGGIALYEPIGKILIPGDVVYA
                     DYAIGRFDLFGANPAQLKDSLNTLSKLDVDILLPGHNQIVKGVPADYIRKTTKMWEPY
                     LV"
     gene            complement(407..>1623)
                     /locus_tag="PKJ17_10940"
     CDS             complement(407..>1623)
                     /locus_tag="PKJ17_10940"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOD76537.1"
                     /translation="SEKTYDKPIVIARATEPVPGTDAAIDCLFPLQFSMQPKELENDK
                     VDFRELKLIYAVAAGDILARKIPATRGEPGMTVVGTAIPAKPGKDIVFTAGRNTIISP
                     DGMEVTSAIAGQPFLKGRSVFVEPVYSIKGDINYSVGNIDFKGSVKIEGNVVSGFSIK
                     ATESIEVNGVVEDCSLEAGMDISVKGGVLGADRGIMKAGRDLSALFVENCHVEAGRNI
                     VVGDTLNSELFAGDSIDVALGTGRVIGSRLSAQNLVTMNTLGSPIAGRTQVSVGFEPK
                     TLALLKELKEEAEKTERTIEEIRKHISTLEEMSLAGPLPDEKERLYGRLLATADELEH
                     QLQEQRGRVEMIESTMTKAAQPTVRVRGACYPNARIKIGTQVFDCTTEYHSAVFYEEE
                     GRIKVNVYDNKT"
BASE COUNT          295 a          490 c          451 g          387 t
ORIGIN      
        1 cgaaggcggc gagacgctgg agataggcaa catggtgtgg gacgtgatac acatacccgg
       61 ccattccatg ggagggatcg ctctctatga gcccatcggg aagatcctta tccccgggga
      121 tgtggtctac gccgactacg ccatcggccg cttcgatctt tttggcgcga atccggccca
      181 gttgaaggac tcgctcaaca cgctgtcaaa gctcgacgtc gacatcctcc tgcccggcca
      241 caatcagatc gtgaagggcg tccccgccga ctacatacga aagaccacga agatgtggga
      301 accatatctc gtttaaaaga aggttcaagg gttcaggaga aaggcaccgt gcctgcggtg
      361 tacgtgtgca gattgtgttt ttttctcttg aactcttgaa cgttttttac gtcttgttat
      421 cgtagacgtt caccttgatc ctgccttctt cttcataaaa gacggcgctg tggtattctg
      481 tcgtgcagtc gaagacctgt gtgcctatct ttatcctggc attggggtag caggcacccc
      541 gcaccctgac ggtcggctgc gcggcctttg tcattgttga ctctatcatt tcgacccggc
      601 ccctctgttc ctgcaactgg tgctccagtt cgtccgctgt cgcgaggagc ctgccataga
      661 gcctctcctt ttcatccggg agtggaccgg cgagactcat ttcttcaagg gttgatatgt
      721 gtttccggat ctcctctatg gtgcgctccg tcttttcggc ctcttccttc aattccttca
      781 ggagggccag cgtcttcggt tcgaacccca ccgatacctg tgtcctgccg gcgatcgggg
      841 accccagggt attcatggtg acaagatttt gcgcggagag cctgctgccg atgacgcgcc
      901 ccgtgcccag ggcgacatcg atggaatcgc ccgcgaacaa ttcggaattg agggtatcgc
      961 cgacgacaat gttgcgtccc gcctccacgt ggcagttctc gacaaagagc gcggagagat
     1021 cccttcctgc cttcatgatg cccctgtccg cgccgagcac gccgcccttg acagagatgt
     1081 ccatcccggc ttcaagcgag cagtcttcga cgacgccgtt cacctcgatg ctttccgtcg
     1141 ccttgatgga gaacccggac acgacattgc cctcgatctt cacgctgccc ttgaaatcga
     1201 tgttgccgac ggagtagttg atatccccct tgatgctgta gacgggttcg acgaacacgg
     1261 accttccctt caggaacggc tgcccggcta tggcagaggt cacctccatg ccatcggggg
     1321 agatgatcgt gttcctcccg gcggtgaaaa cgatatcctt gccgggtttt gccggtatcg
     1381 ccgtgcccac gacggtcatg cccggctcac cccgggtcgc cggtatcttc ctcgccagga
     1441 tgtcccctgc ggccacggca tagatgagct tgagctccct gaagtcgacc ttgtcgttct
     1501 ccaattcctt cggctgcatg gagaattgaa gagggaacag gcaatcgatg gcggcgtccg
     1561 tcccggggac gggctccgtc gccctggcga tcacgatggg cttgtcgtag gtcttctcag
     1621 aca
//
LOCUS       DAOPOA010000608         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_82275_length_1617_cov_2.579385, whole genome shotgun sequence.
ACCESSION   DAOPOA010000608 DAOPOA010000000
VERSION     DAOPOA010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82275_length_1617_cov_2.579385"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKJ17_10945"
     CDS             <1..443
                     /locus_tag="PKJ17_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOD76538.1"
                     /translation="EARSRFIEEEYSRKLAHFQGNAGKEYLEARNAFLRAIFDRARQT
                     VLSWPADEYGRVMKGFLEKITQGRKGRIRIHPDDREVFSKLLAGITAGGGEGDVEIDD
                     GSLPERGGFIFISDEFEVDATIETVLQDIERSLLPEIAEDLARE"
     gene            440..1444
                     /locus_tag="PKJ17_10950"
     CDS             440..1444
                     /locus_tag="PKJ17_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOD76539.1"
                     /translation="MRLSAGYMIPFIKNRPDWGFVCGRMSALEGKLLPREFFTLIIEM
                     HHTEDVYQHLQGTLIEDYLDPGIPWDDFSAIADECFYDLATSIRAECPSPVPADLFLI
                     RNDYLNIKSALSGIDSRFLPGMIREQTIGAVLDGDYADLPSPFRDRITGAGIDLGELD
                     ESMSDMFVDGAYLRHLLMLADEIDSPLIRSCVQERVLGHAVSSLWRVLRQGRDLKQVV
                     DLFLPVGEENPLILDLAGTPDPAAWPDLVGGEIGDQLARAIQMPYDDQISTFELKVTN
                     FVLHLSAYARLETSGPERVFAFLMALEAEMQNLKLVVTGKINRIHEDILGPRLRYVYG
                     "
     gene            1437..>1617
                     /locus_tag="PKJ17_10955"
     CDS             1437..>1617
                     /locus_tag="PKJ17_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOD76540.1"
                     /translation="MAKACIVGEKHLILGFKGIGFEIVAVSDPKDLAEELFMLSRRSD
                     IGLILVTESIAAEAPG"
BASE COUNT          335 a          482 c          485 g          315 t
ORIGIN      
        1 atgaggcccg ttcccgcttc atcgaggagg agtattcccg gaaactggcc cacttccagg
       61 gaaatgccgg gaaggagtac ctcgaggcga ggaacgcctt tcttcgggcc atcttcgaca
      121 gggcccgcca aacggtgctt tcctggcccg ccgacgagta tgggcgggtg atgaagggtt
      181 tcctggaaaa gatcacccag ggccggaagg gacggatacg gatccacccc gacgacaggg
      241 aggtgttctc gaaactcctc gcaggcatca ccgccggggg cggcgaggga gacgtggaga
      301 tcgatgacgg ttctctcccg gaaaggggag gcttcatctt cataagcgat gaatttgagg
      361 tggacgcaac gatagagaca gtattacagg acatagagcg gtcgcttctg cccgagatcg
      421 ccgaggacct tgcacgcgaa tgagactgtc tgccggctac atgatacctt tcatcaagaa
      481 ccgtcccgac tggggtttcg tctgcggcag gatgagcgcc cttgaaggaa agctgctgcc
      541 gagggaattc ttcaccctca tcatagagat gcatcatacg gaggacgtat accagcacct
      601 tcaggggacg ctgatcgagg actatctcga tcccggtatc ccctgggacg atttcagcgc
      661 catagccgac gaatgcttct acgacctcgc cacctcgatc agggccgagt gcccttcccc
      721 cgttcccgct gacctctttc tcatacggaa cgactatctc aacataaaga gcgctctttc
      781 cggtatagac agccgctttc tgccgggaat gatcagggag cagacgatag gcgccgttct
      841 cgacggagac tatgccgacc tgccctcccc cttcagggac cggatcacgg gcgccggaat
      901 agacctcggc gagctcgatg aatccatgtc cgacatgttc gtcgacgggg cgtatcttcg
      961 ccacctgctc atgctggccg acgagatcga cagtcccctc attcgctcct gcgtccagga
     1021 gcgcgtcctg ggacatgccg tttcgtccct gtggcgggtg ctcagacagg gtcgcgacct
     1081 gaaacaggtt gtcgatcttt tcctgcccgt gggagaggaa aaccccctca tcctggacct
     1141 ggcggggaca ccggatcccg cagcctggcc cgacctggtg ggaggagaga tcggggacca
     1201 gctggcccgc gccatccaga tgccttacga cgaccagatc tccacgttcg agctcaaggt
     1261 gacgaatttc gtccttcacc tctccgccta cgcgcgcctc gaaacatcgg ggcccgaacg
     1321 ggtattcgcc tttctcatgg cccttgaggc ggagatgcag aacctcaagc ttgtcgtcac
     1381 gggaaagatc aacaggatac acgaggatat cctgggtccg cggttgaggt atgtctatgg
     1441 ctaaggcctg tattgtcggc gagaaacacc tgatcctggg cttcaagggc atcgggttcg
     1501 agatcgtggc cgtatcggac ccgaaggatc tcgccgaaga actcttcatg ctttcgcgca
     1561 ggagcgacat agggctcatc ctcgtcaccg agagcatcgc ggcggaggca ccggggg
//
LOCUS       DAOPOA010000609         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_82339_length_1616_cov_2.941063, whole genome shotgun sequence.
ACCESSION   DAOPOA010000609 DAOPOA010000000
VERSION     DAOPOA010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82339_length_1616_cov_2.941063"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PKJ17_10960"
     CDS             <1..793
                     /locus_tag="PKJ17_10960"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOD76541.1"
                     /translation="KTAALISMLLVAAAVAGCDVLQDHFIYFPDHVRPSAGALTARGL
                     TVWMASGDDYRGLVSAPESDAPAGTVIVFHGNAGKAADRGFYAKMLVPLNYRVILAEY
                     PGYGGRPGSRGEDQFVKDARLTVEAAFAKYGAPLYLLGESLGCGVVAAVAGGSPVPVE
                     GIVLFTPWDDLLSVAASHAPAFIARIVLKDRYDSVANLANYAGKTAIIGAERDDIIPV
                     VHAEALYASIPGRKRMWTIKGAGHNDWPMLASGAMFREIMDFVSR"
     gene            1028..>1616
                     /locus_tag="PKJ17_10965"
     CDS             1028..>1616
                     /locus_tag="PKJ17_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOD76542.1"
                     /translation="MANVNETDFYDKVFPVPDRVREKSYIKSRAEYDKLYKESIENPD
                     AFWAKEAEKRLTWFKPFDKNKVSDWSFGDDLHVKWFEGGKLNASYNCLDRHLETKKNK
                     AAIIFEGNDPNDWKVFTYWDLYREVNKFANVLKGLGLKKGDVASIYLPMIPELVISML
                     ACARIGVVHAIVFGGFSAEALRDRIKDCKSTVLITC"
BASE COUNT          345 a          459 c          482 g          330 t
ORIGIN      
        1 gaagacagcg gcgcttatat cgatgctgct cgtggcagcg gcggttgcgg ggtgtgatgt
       61 gctgcaggac catttcatat actttcccga tcacgtccgg ccctccgccg gggccctcac
      121 ggcgcggggc ctaaccgtct ggatggcgtc gggggatgat taccgggggc ttgtcagcgc
      181 gccggagtca gacgccccgg caggcacggt cattgtcttt catggcaatg cgggcaaggc
      241 ggccgaccgc gggttctacg cgaagatgct cgtccctctc aactaccgtg tcatccttgc
      301 cgagtatccc gggtatggag ggcggccggg aagccgggga gaagaccaat tcgtaaagga
      361 tgcccgcctt accgtcgagg ctgccttcgc aaagtacggc gcgcccctgt accttttggg
      421 agaatccctc gggtgcggtg tcgtggccgc cgtggccggc ggctcgcccg tgcccgtgga
      481 ggggatcgtg cttttcacgc cctgggatga cctcctgtcc gtggcggcct cgcatgcccc
      541 cgctttcatt gcccggattg tcctgaagga ccggtacgac agcgtggcga acctggcaaa
      601 ttatgccggt aagacggcga tcatcggagc cgagcgcgac gacatcattc ccgtggtcca
      661 tgctgaggcg ctctacgcgt caattcccgg gagaaagcgc atgtggacca taaagggcgc
      721 cggacacaac gattggccca tgctcgcgag cggcgcgatg ttcagggaga taatggattt
      781 cgtctcgaga tagttccgga ggacctttgc cttccttgac gcccgcgccg cggccggtct
      841 tttctgtgca ggagcgtcgg tccggcgact atggtttgta ggcgtttaac ccacatctaa
      901 ttcatcccca tcccgatacc aaacactcat gatttattct aacctgatac cgtgggattg
      961 gtaattgatg agagaatgcg gctgtgagtg ccgtatcggc aaatcttgaa ggacaaggag
     1021 aagaagaatg gcaaacgtca acgaaaccga tttttatgac aaggtgtttc ccgttcctga
     1081 cagggtaagg gaaaaatcgt acatcaagag cagggcggag tacgataagc tctacaagga
     1141 gtccatcgaa aaccctgacg ctttctgggc gaaagaggcc gaaaaaaggc tcacctggtt
     1201 caaacccttc gacaagaaca aggtctccga ttggtctttc ggcgacgacc tccacgtgaa
     1261 atggtttgag ggcggcaagc tcaacgccag ctacaactgc ctcgaccggc acctggaaac
     1321 gaaaaagaac aaagcggcca tcatctttga aggcaacgac cccaatgact ggaaggtctt
     1381 cacctactgg gacctgtacc gggaagtgaa caagttcgcc aacgtcctga agggcctcgg
     1441 cctcaaaaag ggtgatgtcg cttcgatcta tctccccatg atccccgaac tcgtcatctc
     1501 catgcttgcc tgcgccagga taggcgtggt gcacgccatc gtcttcggcg gtttctcggc
     1561 ggaggccttg agagacagga tcaaagactg caaatccaca gtgctcatca cctgcg
//
LOCUS       DAOPOA010000610         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_82662_length_1612_cov_2.120103, whole genome shotgun sequence.
ACCESSION   DAOPOA010000610 DAOPOA010000000
VERSION     DAOPOA010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82662_length_1612_cov_2.120103"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKJ17_10970"
     CDS             <1..634
                     /locus_tag="PKJ17_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOD76543.1"
                     /translation="LVIVDASRGIRLMRSGEEHNEGGKVHGRGHHDHGAFDPHIWLDL
                     GNAMEMVDTITEGLCSADREECDLFRKNAAAYKERLSMLDGKFRETLGTCRKKTLIHG
                     GHFAFGYLAHRYGLEYVSAYEGSPNAEPTARKIIALKKKMDENGIRYVYFEELINPKV
                     SELLSRETGAALLYLHGAHNLTKDEYEKGATFISLMENNLENLRKGLECR"
     gene            625..1386
                     /locus_tag="PKJ17_10975"
     CDS             625..1386
                     /locus_tag="PKJ17_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOD76544.1"
                     /translation="MQMTGVVTTENLSFRFNGVEILSDITFALEKGEFLGIVGPNGSG
                     KTTLIRLLLGLLKPTQGTVMLFGHNSDAFSQWRKIGYLPQKIAAFNPHFPATVEEIIA
                     LGLLAGKSFPRRVARTDRGSIDEVMTFLDITSIRNKLIGELSGGQQQRVLIARALVAG
                     PELLILDEPTTALDPEGRERFFTTLRDLNRRKAVTIIMITHDIGTIGQHASRLLYLDR
                     RLIFFGGFDDFCLSTDMTDYFGEHSQHMICHRHDT"
BASE COUNT          365 a          476 c          455 g          316 t
ORIGIN      
        1 cctcgtcatc gtcgacgcga gtcggggcat cagactgatg agaagtggtg aggagcacaa
       61 tgagggcggg aaggtacatg gccgcggaca tcacgaccac ggcgctttcg atccccacat
      121 ctggctggat ctcgggaacg cgatggaaat ggtcgatacg atcacggaag gtctgtgctc
      181 cgccgaccgt gaggaatgcg acctcttccg gaagaacgcg gccgcctaca aggaacggct
      241 ttccatgctc gacggaaagt tccgcgagac gctggggacg tgcaggaaga agaccctcat
      301 ccacggcggc catttcgctt tcgggtatct cgctcacagg tacggcctcg aatacgtgag
      361 cgcctatgaa ggatctccga atgccgaacc gacggcgaga aaaataatcg ctctcaagaa
      421 gaaaatggac gagaacggca tccgttacgt ctatttcgaa gaactgatca acccgaaggt
      481 ctccgagctc ctctccaggg aaaccggggc ggcactcctc tatctccatg gcgctcacaa
      541 cctgacgaag gatgagtacg agaagggcgc gaccttcatc tctctcatgg agaataacct
      601 cgaaaacctc aggaaaggtc ttgaatgcag atgacgggtg tagtgacaac ggagaacctg
      661 tctttccggt tcaacggcgt cgagatattg agcgacatca cctttgccct cgaaaagggg
      721 gaattcctgg ggatcgtcgg accgaacggc tccggcaaga cgaccctcat ccgcctgctt
      781 ctcggcctgc ttaagcccac acagggtacg gtcatgctct tcggacacaa ctcagacgcc
      841 ttcagtcagt ggcggaagat cgggtacctt ccccaaaaga tagcggcttt caacccgcat
      901 tttcctgcca cggtggaaga gatcatcgcc ctggggctgc tggccggtaa aagtttccca
      961 aggagggtcg cgcggaccga caggggatcc atagacgaag tcatgacctt cctggatatc
     1021 acatccatca ggaacaagct catcggggaa ctctcagggg gccaacagca gcgcgtactg
     1081 atagccaggg cactggtggc cgggccggaa ctcctcattc tggacgagcc gactaccgcc
     1141 ctcgatcccg aggggaggga aaggttcttc acgaccctca gggacctcaa caggcgcaag
     1201 gcggtaacga tcatcatgat aacccacgac atagggacca tcggccagca cgcttcgaga
     1261 ctcctctatc tcgacaggag gctcatcttc ttcggcggct tcgacgattt ctgcctctcc
     1321 acggacatga cggactactt cggcgagcac tcccagcata tgatctgtca caggcatgac
     1381 acgtgatgat gattgaccaa tcaccaatga ccacgagcca aactgtcgcc ggatgtgtct
     1441 gggcgggcgc gccatacctt attccgtctt ctcattcatt gcccggaggg gccgttggac
     1501 ttccttgacc ttttcagcca tgggttcatc cagcgggccc tgcttgcggg ctccttcata
     1561 gccgcgctct gttcggcgct ggggacgttt ctcgtcctgc gcaggctttc ac
//
LOCUS       DAOPOA010000611         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_82911_length_1608_cov_3.285254, whole genome shotgun sequence.
ACCESSION   DAOPOA010000611 DAOPOA010000000
VERSION     DAOPOA010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82911_length_1608_cov_3.285254"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKJ17_10980"
     CDS             complement(<1..190)
                     /locus_tag="PKJ17_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76545.1"
                     /translation="MMNGTPSKGIFYAPAVLFVIVSVSPEASSARPQGGVKRLVEQHA
                     RPGKEARYPEAAAIAEKVL"
     gene            679..1374
                     /locus_tag="PKJ17_10985"
     CDS             679..1374
                     /locus_tag="PKJ17_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76546.1"
                     /translation="MGRAVSPHTCRAMARKIVLGQYARPGREYIRALAKAIAGPYAHL
                     DHPLGEDIVLAKLRAVRRGDGVEYLGWRYMERIFTSRDNDHPYFTIFTYLDETDIAID
                     VAEMLSLDPAPEIVDALAENIVRRSFRDRAVHGWELPNWIGDFLCEAGKESDMVFAAA
                     TVVLTFVSLREKIFSFSRSAEFARSDDLPGLEAYMAKFLEGYKAQGLLHSYTKKDLED
                     IVEAIRKRAREKT"
BASE COUNT          362 a          454 c          451 g          341 t
ORIGIN      
        1 ccaacacttt ctctgcaatt gcagcggctt cagggtaacg ggcttctttg cccggccggg
       61 catgttgttc gacgagtctt tttaccccgc cctgcggccg ggccgaagat gcttcaggag
      121 agacggacac aatgacaaac aagacagctg gtgcgtaaaa gattcctttg cttggggttc
      181 cattcatcat ttacctcatc tctgtatgga taacgatata gccgaaacat atatcgcgat
      241 ggaacggcaa aacagagaat cccttgcagt gtgtattata ctgtatgaca ttattgattg
      301 ctgccagtca gcaactttct tcaatcacct gaaattcctt ctcgtaccgt acctggaatc
      361 cggaccggac gcgccctgcc ttaacccatg cccgcggagc cgagccccct ggcaggcggc
      421 atgaaatatt tgattgcagt ttattacggt aatataatat aatacattgt attatgaatc
      481 aagggtgctt attatagccg gaatgtccat taacggtaat acacggaggg cctgatggga
      541 aagcggggaa ggccgggacg gaacccctac gatgtgctca ggatgtacag ggtatacgcc
      601 atcttcgaga acgaagacgg ttccttcccc ataacgtact actagctcgg aaagcggata
      661 acgaaggcat tcgggatcat gggcagggcc gtgtcgccgc atacctgccg ggcaatggcc
      721 cgaaagatag tgctcggcca atacgcccgt cccggccggg agtacatcag ggccctggcg
      781 aaggcgatcg cggggccgta cgcgcacctc gaccaccctc tcggcgagga catcgtcctt
      841 gccaagctaa gggcggtgag gaggggggac ggcgtcgagt acctcggctg gaggtacatg
      901 gaacggatct tcacgtcccg ggacaacgac catccctatt tcaccatctt cacctatctc
      961 gacgagaccg acatcgccat agacgtcgcc gagatgctaa gcctcgatcc cgcccctgaa
     1021 attgtggacg ccctcgcaga gaacatagtc aggcggtcct tccgggacag ggccgtccac
     1081 ggatgggagc tgccgaactg gataggggat tttctgtgtg aggccggaaa ggaatcggac
     1141 atggtcttcg ccgcggcgac cgtcgtgctc accttcgtct cgctgaggga gaagatattc
     1201 tcgttctcga ggtccgccga gtttgcacgg tcggacgacc ttcccggcct tgaggcctac
     1261 atggcgaaat tccttgaagg gtacaaggcg caggggcttt tgcattccta tacgaaaaag
     1321 gacctggagg acatcgtgga ggcgatccgc aagagggcac gggaaaagac ctgacggcct
     1381 ccctggttcc ggcaggccgg gtggaggggc gtaaagttct tgcagtccgc ggcgctcgga
     1441 tcacttttat gtttttaaag gatagggacc aaagtgtaat cctccatctc cttcaaagac
     1501 atgccccgtc acaccggccg acctgttaca cgtcccccgc cttcaacctc gtcctcacgg
     1561 tcgactcggg gacccccagc ctccgggcga ttcccctgaa cgacattc
//
LOCUS       DAOPOA010000612         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_83427_length_1601_cov_2.837646, whole genome shotgun sequence.
ACCESSION   DAOPOA010000612 DAOPOA010000000
VERSION     DAOPOA010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83427_length_1601_cov_2.837646"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PKJ17_10990"
     CDS             complement(<1..773)
                     /locus_tag="PKJ17_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOD76547.1"
                     /translation="MTPNHDFQTVRTLITRNAALYPQKMAMKEFETGRNCTFEDLLER
                     AKRIGSALHGMGVKKGDRVGIMSQNSYEYMELILSVTAAGYVFVPVNFRLAGREMAGV
                     LSDAGPVVLFVHEQYVKTAHEIKGEIPSVREYVHIGPAETRPDGWRGYEEMIQCAAPM
                     GWVESVEEGDLAMLMYTSGTTGAPKGVMQTQLNLYHCGRTCAFNNCIDTEDIGFTICP
                     MYHVTATTSFSVPSTGERQASSSRSSTPRPSSRRPGRSAS"
     gene            complement(1220..>1601)
                     /locus_tag="PKJ17_10995"
     CDS             complement(1220..>1601)
                     /locus_tag="PKJ17_10995"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOD76548.1"
                     /translation="VAEWVVSLPVHRHIIMIFIFLIYLIGGSFIDDLAFLILATPIFY
                     PAITNMGYDPIWACIMLSLTVCVGSVIPPVAMCVFVVKNITKVPMGVIYKGVYPFLIA
                     LFLCVALLFVFPELTLYLPSVLMK"
BASE COUNT          345 a          479 c          452 g          325 t
ORIGIN      
        1 gatgcgctcc tccctggccg cctcgaagag ggcctcggcg tcgaacttct ggaagaggat
       61 gcttgtcgct ccccggtaga agggaccgaa aaggatgtcg ttgccgtgac atgatacatg
      121 gggcatatcg tgaagccgat gtcctcggtg tcgatgcagt tgttgaaggc acaggtccgt
      181 ccgcagtggt agaggttgag ctgggtctgc atgacaccct tgggcgcgcc cgtcgtcccg
      241 ctcgtgtaca tgagcatggc gaggtcgcct tcctcaacgg actccaccca tcccatcggg
      301 gcggcgcact ggatcatctc ctcgtatccg cgccatccgt cgggccgcgt ctccgcaggc
      361 cctatatgca cgtactcacg caccgagggg atctcaccct ttatctcatg cgccgtcttg
      421 acgtattgct cgtggacgaa aaggaccacg ggcccggcgt ccgacaggac ccctgccatc
      481 tcccgccctg ccagcctgaa gttcaccgga acgaagacat atcccgccgc cgtcaccgac
      541 aggatcagct ccatgtattc gtagctgttc tggctcatga tgccgacgcg gtcgcccttc
      601 ttcaccccca taccgtgaag ggcgctcccg atcctcttcg ccctctccag caggtcctca
      661 aaggtacagt tccttcctgt ctcgaattcc ttcatcgcca tcttctgcgg atacagagct
      721 gcgttgcggg tgatcaacgt ccttaccgtc tgaaagtcat ggttcggggt catttccagt
      781 cgcctcctga agtgcagtaa tagagtgaga ttcgcacatc atcatgagca attagcatgc
      841 cactgaaaag acggtcgcag gtcgcaggtc gcaggtcgca ggttggagac agcagacggg
      901 gcgggtggcg gcggaccctt gctatccaat tgatttcggg gtattacgtc gatgtcacgg
      961 aaacgacgaa caacagcggg acgcgcgtgc ccgggaaccc ggcaccccca tcgatctccc
     1021 gcctgtgcat tttctgcaca cctgttcaat ttcattacat aaagacatcc ctgcagacac
     1081 cccccttcac acccgttctt ctctcttcta ccctcaagcc attcaagcca ttcaagcggt
     1141 tcaagccgtc tttagaaact ttgggccgga tggcagcgct ccatccggcc cgggtatcga
     1201 aggagtgtat acgaacggcc tatttcatga gaaccgacgg cagatacagc gtcagttcgg
     1261 ggaagacgaa aagcaaggcc acgcagagga acagcgcgat gaggaagggg tagacgccct
     1321 tgtagataac gcccatgggg accttggtga tgttcttcac cacgaagacg cacatggcga
     1381 cgggaggaat gacggagccg acgcagacgg tgagggacag catgatgcac gcccagatcg
     1441 gatcgtatcc catgttcgtg atcgccgggt aaaatatcgg tgtcgcgagg atgaggaacg
     1501 caaggtcatc gatgaaggag ccaccgataa ggtaaatgag gaagatgaag atcatgatga
     1561 tgtggcgatg gaccggtagg ctcaccaccc actccgccac c
//
LOCUS       DAOPOA010000613         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_83653_length_1598_cov_3.218406, whole genome shotgun sequence.
ACCESSION   DAOPOA010000613 DAOPOA010000000
VERSION     DAOPOA010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83653_length_1598_cov_3.218406"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /gene="ade"
                     /locus_tag="PKJ17_11000"
     CDS             <1..1535
                     /gene="ade"
                     /locus_tag="PKJ17_11000"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOD76549.1"
                     /translation="IDRERYWYDTYILPGFVDSHVHVESSMLTPAGFARLAVVHGTVA
                     TVSDPHEIANVLGPQGVRFMMEDAAGIPFVFAFGVPSCVPASPFETSGAAMGLAEMEA
                     LFDEQGAGYLSEMMNVPGVIGGDPDVQAKIEAARRRGKPVDGHAPGLRGEGLRRYIAA
                     GISTDHESFELEEAREKVSLGMKILVREGSAARNFDTLYPLIDESPGFCMLCTDDQHP
                     DSLVKGHINTLVARAIRKGVDVMKVLRCACVNPVLHYGLDVGLLRPSDPADMIVVDGL
                     EDLEVLKTIVRGEIVAEAGESRIPHRRATPVNIFRATVRKPEDFRVLKRSDAIRVIGV
                     IEGQVVTYAHLEAPALEGDALACDTDRDILKIAVVNRYADRPPAVAFVRGFGLKKGAM
                     ASSIAHDSHNIVAVGTSDEMLCDAVNLVIEKKGGISLVSEEMKMVLPLPVAGLMSNED
                     GYSVAERYSRLTLLARDLGTTLKAPFMTLSFMALVVIPKLKLSDKGLFDGEKFTFTDL
                     FV"
BASE COUNT          314 a          450 c          489 g          345 t
ORIGIN      
        1 gcattgacag ggagcgctac tggtatgata cctatatcct tcccggtttt gttgattccc
       61 acgttcacgt tgagagttcc atgctcacgc cggccggttt cgcccgcctt gccgtcgttc
      121 acggaacggt ggcgaccgtt tccgaccccc atgaaatagc caatgtcctg gggccgcagg
      181 gcgtgcgttt catgatggag gacgccgcgg gaataccctt cgtttttgct ttcggcgtgc
      241 cctcctgcgt tcccgcgagt ccctttgaga cgtcgggcgc cgcgatgggc ctcgccgaga
      301 tggaggccct ctttgacgag caaggcgctg gatatctcag cgagatgatg aacgtccccg
      361 gtgtcattgg cggcgacccg gacgtgcagg cgaagataga ggcggcaaga cgaagaggaa
      421 agccggtgga cgggcacgca ccgggccttc gaggagaagg gttgcgcagg tacatagcag
      481 cgggcatatc cacggaccac gaatcctttg agcttgagga ggcgcgcgag aaggtctccc
      541 tgggtatgaa gatactggtc cgtgaaggtt cggcggcaag gaacttcgat acgctctatc
      601 ccctcatcga cgaatctccg ggcttctgca tgctctgcac cgatgaccag caccccgatt
      661 ccctcgtaaa gggacacatc aacacacttg tcgcccgggc catcagaaaa ggcgtggacg
      721 ttatgaaggt cctgaggtgt gcctgtgtca accctgtcct tcactacggt ctcgacgtgg
      781 gtctgctcag accttctgac cctgccgaca tgatcgttgt cgacggtctt gaggaccttg
      841 aggtcttgaa gaccatagta cgcggcgaga tcgttgccga agccggtgaa agccgcatac
      901 cgcatcgcag ggccaccccg gtcaacattt ttcgggcgac cgtcaggaaa ccggaggatt
      961 ttcgggtcct caagaggtcc gacgccattc gtgtcatcgg ggttatagaa ggtcaggtcg
     1021 tgacctatgc tcacctcgag gctcccgccc ttgaggggga cgcactggcc tgcgatacgg
     1081 acagggacat actgaagata gctgttgtga accgctatgc agatcggccg cccgcggtgg
     1141 ccttcgtgag gggattcggc ctgaagaaag gcgccatggc ctcgtccatt gcgcatgatt
     1201 cccacaatat cgtcgctgtg ggcacgagcg atgagatgct ctgcgacgcc gtcaatcttg
     1261 tcatcgagaa gaagggcggc atctcgcttg tctcggagga gatgaagatg gtgctgcccc
     1321 ttcccgtggc gggtctgatg tcgaacgagg acggttacag tgtcgccgaa aggtacagcc
     1381 gtctcacctt gcttgcccgg gaccttggca cgacgctcaa ggcgcccttt atgacgcttt
     1441 ccttcatggc cctcgtggtg atcccgaaac tcaaactgag cgacaaggga ctcttcgacg
     1501 gggagaagtt cactttcacg gacctctttg tgtgaaaggg ttcgacttgc cgttttaatg
     1561 ggttcaatag gttacatggg tcccatatga cccgggaa
//
LOCUS       DAOPOA010000614         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_83937_length_1594_cov_4.078622, whole genome shotgun sequence.
ACCESSION   DAOPOA010000614 DAOPOA010000000
VERSION     DAOPOA010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83937_length_1594_cov_4.078622"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PKJ17_11005"
     CDS             <1..956
                     /locus_tag="PKJ17_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76550.1"
                     /translation="VTNGELTLEAFVYDTLDGSMVLAKRYKARQSEWRRVTHRLADDI
                     IFAVTGEKGIMSSRVLFTAGRRNLKEIYLSDFDGQNVTRLTNYRSITLSPSLSPNGKY
                     LAYTSYREGRPNLYVTDFDKKNEIFVDRSEGMKIGTSWLGNSTLVYSHTSGKTSTIYA
                     FDVGSRSKKVLVRRDGILTSPTVSPDGSRLIFVSDMFGTPQVFSKTLPSGEPKRLTYH
                     GRYNSAPAFSPKGDLVAFVANISGGFEICTMNADGSNQRVLTNDGTVNDSPQFSSCGR
                     YIIYTSLKGGVNRLNIMLFNGDNKRTLRFTDFAEEQPRFAP"
     gene            1011..1553
                     /locus_tag="PKJ17_11010"
     CDS             1011..1553
                     /locus_tag="PKJ17_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOD76551.1"
                     /translation="MKKILILLVALIFVAVGCAPKAVQTTPPATSVTDRSLSDKDKDR
                     TGISEEELAKAERDRLLREQERLAGILKDVHFDFDSYAVNSSELSKIEGVGSFMKGDR
                     SVRIVIEGHCDERGTVDYNLALGQKRAEAVKAYLLKLGIESSRIRTISYGKELPADTG
                     KTEEAWARNRRAHFKLDQKG"
BASE COUNT          392 a          448 c          462 g          292 t
ORIGIN      
        1 ccgtgacgaa cggcgagttg accctggagg cgtttgtcta tgacaccctg gacggctcga
       61 tggtgctggc aaaacgctac aaagcgaggc agagcgagtg gaggagggtg acgcaccggc
      121 tggctgacga catcatcttt gccgtcaccg gcgaaaaggg catcatgagc tcccgcgtac
      181 tcttcacggc gggccgcagg aacctgaaag agatctacct gtccgacttc gacgggcaga
      241 acgtgacgcg cctgaccaat tacagaagta tcacgctttc cccatccctg tccccgaatg
      301 ggaaatacct cgcctacacg tcctaccggg agggcagacc caacctttat gtcaccgatt
      361 tcgacaagaa gaacgagata ttcgtcgatc gttcggaagg catgaagatc ggcacatcgt
      421 ggcttggcaa ctcgacgctc gtctattccc acacgtcggg caagacgtcg accatctacg
      481 ccttcgacgt ggggagccgc agcaaaaagg tcctcgtccg gcgcgatggc atactcacct
      541 cacccacggt ctcgcccgac ggctcgcggc tcatcttcgt atccgacatg ttcggaacac
      601 cccaggtctt ctccaagacg ctgccttcgg gcgagccaaa gagactaacc tatcacggca
      661 gatacaacag cgccccggct ttctcgccca agggtgatct cgtcgccttc gttgcgaaca
      721 tatcgggggg gttcgagata tgcacgatga acgccgacgg ttcgaaccag cgggtgctca
      781 ccaatgacgg cactgtgaat gattcaccgc agttctcttc ctgcggccgc tacatcatct
      841 atacatcctt gaagggaggc gtcaacaggc tcaacatcat gctcttcaac ggcgacaaca
      901 agaggaccct gcgcttcacc gattttgccg aggaacagcc gagatttgcg ccataggggg
      961 caaggaggga ataacaagag gaagggcaca acacagaacg gggaggattt atgaaaaaga
     1021 ttctgattct tctggtggca ttgatcttcg ttgccgtcgg gtgtgcgccg aaagcggtcc
     1081 agaccacgcc ccccgcaaca agcgtaacag acaggtccct gtccgacaag gacaaggaca
     1141 gaacgggcat cagcgaagaa gagctcgcga aagcggagcg tgaccgcctc ctgagagagc
     1201 aggaacgcct ggccggaata ctcaaggatg tccatttcga ttttgacagt tacgccgtga
     1261 acagttcgga actgtccaag atagagggcg tgggctcctt catgaaaggg gacaggagtg
     1321 ttcggatcgt tatcgaaggg cactgcgacg aacggggcac cgtcgattac aaccttgctc
     1381 tgggtcaaaa gagggctgag gccgtgaagg cgtatcttct caagctgggc atagaaagca
     1441 gccggatccg cacgatatcc tatggcaagg agcttcccgc cgatacaggc aaaaccgagg
     1501 aagcctgggc aaggaacagg cgggcccatt tcaaactcga tcagaagggg tgagcaatgg
     1561 aacatgcatc catcattggc ggcagggggt cttt
//
LOCUS       DAOPOA010000615         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_83944_length_1594_cov_3.363223, whole genome shotgun sequence.
ACCESSION   DAOPOA010000615 DAOPOA010000000
VERSION     DAOPOA010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83944_length_1594_cov_3.363223"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKJ17_11015"
     CDS             <1..574
                     /locus_tag="PKJ17_11015"
                     /inference="COORDINATES: protein motif:HMM:NF021900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOD76552.1"
                     /translation="AKVSSDMRRMSMGPRGGLAEIRLPALQEGSSIMPDKVNPVITEM
                     ATSVAFQVMSLDHAITLAAASGQLELNAFLPLIAFNLLTELKLLANALSIFRTHLVEG
                     VEADERRCRRWLEESLCLATALAPYIGHEKAGELSKAAREEGKTIREAAVEKGLFPEE
                     EIEAIFQARELTRPGIAGSKKVKKNKEGQT"
     gene            571..>1594
                     /gene="hydF"
                     /locus_tag="PKJ17_11020"
     CDS             571..>1594
                     /gene="hydF"
                     /locus_tag="PKJ17_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOD76553.1"
                     /translation="MTDTTLNQTPRGSRLHIAIFGRRNAGKSSLINALTNQDIAIVSD
                     VPGTTTDPVYKSMEILPIGPVVIIDTAGIDDVGELGRLRIEKALGVLGRTDLMLLVVD
                     PASGAGVFEEEVLEKARENKVPVIIVVNKTDLYPEFRTEEVFPGRALPVVAVSALTRQ
                     GIDELKMAMIGEAPKDFMTPTILGDLISPGDTVVLVVPIDLAAPKGRLILPQVQTIRD
                     ILDNDAMAYVVKERELKGALAKLKDKPRLVVTDSQAFLKVAADTPKDVPMTSFSILFA
                     RHKGDLPTLVEGAKAIEDLVPGDRVLVSEACTHHRVEDDIGTVKIPRWLNQLVGGPLD
                     YTWVSGL"
BASE COUNT          340 a          476 c          506 g          272 t
ORIGIN      
        1 cgccaaggtg tcgtcggaca tgcgccgtat gtccatgggt ccgcggggag gcctcgccga
       61 gataaggctg cccgctctcc aggaaggttc atccatcatg cccgacaagg tgaaccccgt
      121 catcacggag atggcgacga gcgtggcctt tcaggtcatg agtctggacc acgccattac
      181 cctcgcggcg gcgtcggggc agctcgaact caacgcgttc ctgcctctta ttgccttcaa
      241 cctcctcacg gaactgaaac tccttgcaaa cgcgctctcc atcttccgca ctcatctggt
      301 cgagggggtg gaggctgatg aaaggcgttg cagacgatgg ctggaagaaa gcctctgcct
      361 tgccaccgcg ctcgccccgt acatcggtca cgaaaaggcg ggcgaactct cgaaggcggc
      421 ccgcgaagag ggaaagacga tcagagaggc cgcagtcgaa aaggggttgt tcccggaaga
      481 ggagatcgaa gccatctttc aggcccgcga gttgacgagg ccgggcatag ccggatcgaa
      541 aaaggtgaag aagaacaagg aaggacagac gtgacggata cgacactcaa tcaaacgccg
      601 agaggaagcc ggcttcacat tgccatcttc ggcaggagaa acgccggcaa gtcgagcctc
      661 ataaacgcat tgacgaacca ggacatcgcc atcgtctccg atgttcccgg gaccacgacg
      721 gacccggtct acaagtcgat ggagatactt cccatcggcc ctgtcgtcat catcgatacg
      781 gcgggtatcg acgacgtggg cgaactgggg aggctccgta tcgaaaaggc cctgggcgtt
      841 ctcggcagga cggacctcat gctcctcgtc gtcgacccgg cctccggcgc gggtgtgttc
      901 gaggaggagg tcctcgagaa ggcgcgcgag aacaaggtcc ccgtcatcat cgtcgttaat
      961 aagacggacc tctatccgga gttcaggaca gaggaggtct tccccggccg ggcgttgccc
     1021 gtcgtggccg tgagcgcgct cacccgccag ggaatcgatg aactgaagat ggccatgatc
     1081 ggggaggcac cgaaagactt catgaccccg accattctgg gagatcttat ctctcccgga
     1141 gacacggtcg tgctcgtcgt gcccatcgac ctcgcggcgc cgaaggggag gctcatcctt
     1201 ccccaggtcc agaccatccg cgacatcctg gacaacgacg ccatggccta cgtggtgaag
     1261 gagcgggagc tgaaaggggc cctggcgaag ctgaaggaca agccgaggct tgtcgttacc
     1321 gattcccagg cgttcctgaa ggttgccgcc gatacgccga aggacgtccc gatgacgtct
     1381 ttctcgatcc tcttcgcccg gcacaaggga gaccttccga cgctcgtgga gggggcgaag
     1441 gcgatagagg accttgtccc cggggacagg gtgcttgttt ccgaggcctg cacgcaccac
     1501 cgcgtggagg acgatatcgg gacggtgaag ataccgagat ggctcaacca actcgtgggc
     1561 ggaccccttg actacacgtg ggtgagcggg ctcg
//
LOCUS       DAOPOA010000616         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_84241_length_1590_cov_2.663844, whole genome shotgun sequence.
ACCESSION   DAOPOA010000616 DAOPOA010000000
VERSION     DAOPOA010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84241_length_1590_cov_2.663844"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /gene="glmS"
                     /locus_tag="PKJ17_11025"
     CDS             <1..930
                     /gene="glmS"
                     /locus_tag="PKJ17_11025"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOD76554.1"
                     /translation="CGTSYHACLIGKHIFEANLRIPVETDIASEFRYRDPILGDNDLV
                     ILVSQSGETADTIAAMKEVKKKGLHTLAICNVLGSTLSRECHSVIYTHAGPEIGVAST
                     KAFTTQVSVFFMLMLYLGKRLGVVDSDDGRRLIGELRKIPHKIQTILDASSSIEEHAR
                     ENMQYRHFLYLGRGINFPTVLEGALKLKEISYIHAEAYAAGEMKHGPIALIDENMPIV
                     FVSPRDQTYKKTCSNMEEVLARKGKVLLFTDNSRHDMADRVDALFVVPESIYELQPIL
                     TIVPLQLMAYYMANLLGTDVDQPRNLAKSVTVE"
     gene            complement(814..>1590)
                     /locus_tag="PKJ17_11030"
     CDS             complement(814..>1590)
                     /locus_tag="PKJ17_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76555.1"
                     /translation="GADITVYIEGDGSAWLSPTLLSGDPTPARPLVLQMAALDPAGNV
                     AYLARPGQYASGPSPGCDPLYWSDRRFSPEVVEAMSEGIEKLRCLSGSGRVDLVGYSG
                     GGAIAVLVAARRSDIASLRTVAGNLDTEAVNRHHGVSFLAGSLNPIDEAARLARLPQR
                     HFAGAKDTVVPVFVVRSFLKQAGDTDTRRLVVLKDVSHSRGWLEKWGDLLALPPITAR
                     QGLLDSHALRQVPGLVDVGAEKVGHVVGHELEGHYGKNGL"
BASE COUNT          354 a          511 c          457 g          268 t
ORIGIN      
        1 tgcggtactt cgtaccacgc ctgtctcata ggcaagcata tcttcgaggc gaacctgaga
       61 ataccggtgg aaaccgacat cgcgtcggaa ttccgatacc gcgaccccat cctcggagac
      121 aacgatctcg tcatactcgt ctcgcaatcg ggagagacgg cggacaccat agcggctatg
      181 aaagaggtga agaagaaagg gcttcacacc ctcgccatat gcaacgtgct gggcagcacc
      241 ctttcaaggg aatgccacag cgtcatatac acccacgcgg gacccgagat aggggtggcc
      301 tcgacgaagg cgttcaccac gcaggtctcc gtcttcttca tgctcatgct ctacctcgga
      361 aaacgtctcg gcgttgtcga cagtgacgac ggaaggcgcc tcatcggtga actcaggaag
      421 atcccgcaca agatccagac gatactcgac gccagttcct cgatcgagga gcacgcccgg
      481 gagaacatgc agtaccgcca ctttctctat ctcggcaggg gcatcaactt cccgaccgtc
      541 ctcgagggag ccctgaagct caaggagatc tcctacattc acgcggaggc gtacgcggcc
      601 ggggaaatga agcacggccc catagccctc atagacgaga acatgcccat cgtcttcgta
      661 tcgccgcggg accagacata caagaagacc tgttccaaca tggaggaggt gctggcacga
      721 aaaggaaagg tcctcctctt cacggacaac tcccggcacg acatggccga cagggtggac
      781 gccctgtttg tcgttcccga gagcatctat gagctacagc ccattcttac catagtgccc
      841 ctccagctca tggcctacta catggccaac cttctcggca ccgacgtcga ccagccccgg
      901 aacctggcga agagcgtgac tgtcgagtag tccctggcgg gcggttatgg gaggaagggc
      961 cagaaggtct ccccacttct caagccagcc ccgggaatgg gaaacatcct tgaggacaac
     1021 aagcctgcgg gtgtcggtat cgcctgcctg ttttaggaaa gaccgtacaa cgaagaccgg
     1081 taccaccgta tccttcgccc cggcaaaatg gcgctgcgga agacgtgcca gccttgccgc
     1141 ctcatcgatg gggttaaggg accccgcaag aaagctcaca ccgtgatggc ggttcaccgc
     1201 ctccgtgtcg aggttacccg cgacagtcct caggcttgcg atatcgcttc gccgcgccgc
     1261 gacgaggacg gcgatggcgc cgcccccgga atacccgacg agatcgaccc tgcccgagcc
     1321 cgagaggcaa cgaagcttct ctatcccctc gctcatcgcc tcgacgacct cgggagagaa
     1381 gcgcctgtcc gaccagtaca ggggatcgca tccgggcgag ggacccgaag cgtattgtcc
     1441 cgggcgagcg aggtaggcga catttccggc cgggtccaat gcggccatct gcagaacgag
     1501 aggtctcgcc ggggtcgggt cccccgagag cagggtcggt gagagccacg cggacccatc
     1561 cccctcgata tacaccgtga tgtccgcgcc
//
LOCUS       DAOPOA010000617         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_84519_length_1586_cov_3.670150, whole genome shotgun sequence.
ACCESSION   DAOPOA010000617 DAOPOA010000000
VERSION     DAOPOA010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84519_length_1586_cov_3.670150"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PKJ17_11035"
     CDS             <1..501
                     /locus_tag="PKJ17_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOD76556.1"
                     /translation="GDEIQLGDVRGTVMKINIRNTVVQTNDDSTIFIPNSDLMYKNIV
                     NWTYRDPRGRAEISVGVAYGSDTEVVRDLLVRCALSHPNVLREPEPYVLFWDFGDSAL
                     VFRLRFWIRRPVQMRDKISSAIRFEIDRVFKENTIEIAFPQQDVHIRSADGLNSLPAP
                     EGKPGT"
     gene            525..>1586
                     /locus_tag="PKJ17_11040"
     CDS             525..>1586
                     /locus_tag="PKJ17_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOD76557.1"
                     /translation="MLKQVGYFDPVPKEDLIGRIDRLRQLMADSGFDLALLLQNVDRF
                     YFTGTMQKGVVAVPLDQDPLVFVEKGTDRAAMETPLNLTPVKNDREIGDILRGRRLLA
                     GKVGLELDVLPVAVFERMKRVFDFERCGDVSEQVREVRAVKSRFELDQIVMSGRMLTS
                     VFSLARDEVREGRTELGIEAALTAAGREMGHQGFLRMRGMNQEMMTMTVQAGFTGAIP
                     TLLDAPITGTGVTPAVPQGSSFKKVERGVPVTIDYGGGYNGYITDETRSFVVGDLAEA
                     FRKPYETARTIIEDVLAVAGPGTDCRDIFHRAEDVAREAGLGDFFMGHGEGKVAFIGH
                     GLGLEINELPILTPRHERIL"
BASE COUNT          324 a          464 c          510 g          288 t
ORIGIN      
        1 ggcgacgaga tccagcttgg cgatgtacgg gggacggtga tgaagataaa catccgcaac
       61 accgtcgtgc agaccaacga cgactccacg atcttcatcc ccaactcgga cctcatgtac
      121 aagaacatcg tcaactggac ataccgcgac ccccggggca gggccgagat atccgtggga
      181 gtggcatacg gctccgacac ggaggtcgtc agggacctgc tcgtccggtg tgccctttcc
      241 catccgaatg ttctcaggga gcccgaaccc tacgtcctct tctgggattt cggagacagc
      301 gccctcgtgt tccgcctgag gttctggata cggcgtcccg tgcagatgcg tgacaagata
      361 tcatcggcga tacgttttga gatagacagg gtgttcaagg agaacaccat cgagatcgct
      421 ttcccccagc aggatgtcca catccgttcc gccgatggtt tgaattccct cccggccccg
      481 gaagggaagc cggggacttg aatctccccc tgacgaggtg gaatatgttg aaacaggtag
      541 gttacttcga tcccgtgccg aaagaggacc tcatcggcag gatcgacagg ttgcggcagt
      601 tgatggcgga ttcaggattc gatctcgcgc ttctcctgca gaacgtcgac aggttctatt
      661 ttacggggac catgcagaag ggcgtcgttg ccgtgcccct tgaccaggac cccctcgttt
      721 tcgtggaaaa gggaacggac cgtgcggcca tggagacacc ccttaacctc acgcccgtca
      781 agaacgaccg ggagatcggc gacatcctcc gcggccgtcg cctcctcgcg ggaaaggtgg
      841 gcctcgagct tgacgtgctg cccgtcgccg tctttgagag gatgaagagg gtcttcgact
      901 tcgagcgttg cggcgacgtg tcggagcagg tcagggaagt gcgggccgtc aagagccgct
      961 ttgagctcga ccagatcgtg atgtccggcc ggatgctgac cagcgtcttc tcgctggcac
     1021 gggacgaggt ccgcgagggc aggacggagc tcggtatcga ggcggccctc acggcggccg
     1081 ggagggaaat gggccaccag gggttcctca gaatgagagg gatgaatcag gagatgatga
     1141 cgatgaccgt gcaggcggga ttcaccggtg ccattcccac cctgctcgac gccccgataa
     1201 cggggaccgg ggtcaccccc gccgtccccc agggttcctc cttcaagaag gtcgaaaggg
     1261 gagtccccgt caccatagac tacggcggag ggtacaacgg gtatatcacc gacgagacga
     1321 ggagcttcgt ggtgggcgac ctggcggagg cgttcagaaa accctatgag acggcacgga
     1381 cgatcataga ggatgtgctt gccgtcgcgg gaccggggac cgattgccgg gatatcttcc
     1441 accgcgccga ggacgtggcg cgcgaggccg gactcggcga tttcttcatg ggacacggcg
     1501 aaggcaaggt ggcgttcatc ggccatggcc tcggcctcga gatcaacgaa ctgcccattt
     1561 tgaccccgcg ccacgaaagg atactg
//
LOCUS       DAOPOA010000618         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_84589_length_1585_cov_3.629412, whole genome shotgun sequence.
ACCESSION   DAOPOA010000618 DAOPOA010000000
VERSION     DAOPOA010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84589_length_1585_cov_3.629412"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..>1585)
                     /locus_tag="PKJ17_11045"
     CDS             complement(162..>1585)
                     /locus_tag="PKJ17_11045"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOD76558.1"
                     /translation="GGYEGTISIEGKKVRFASPFDALEAGIGMVHQEFSLIPGFTVTE
                     NIVLNRESTKYNFLVEAYGDRMKTLDRDDMDRRSREAIDKLKVGLDPAIAVSELPVGY
                     KQFTEIAREIDKENTKILVLDEPTATLTESEAEILLDTMKRLAALGISIIFISHRLQE
                     IMDVCDKIIVLRDGEVVDNTTPDETDIMSIATSMVGRDITTAFAGKGDREGNLGEVIL
                     QVEHLWVDMPGETVRDVSFQVREREIFGIGGLAGQGKLGITNGIMGMFPAGGSVFFLE
                     GPIRLNDPTSPLEAGISAVYEDRRGVGLLMEEPISWNIIFTSLQMQERFLKPILGGLI
                     KIRDESEIARSARDYIDMLNIKCLSENQRVQDLSGGNQQKVCLAKAFETKPRLLFVSE
                     PTRGIDVGAKRMVLEALRKYNDEFGTTIVMVSSELEELRSICDRIAIIDSGRIAGILP
                     ASAPSTDFGVLMLGIDGREKKVV"
BASE COUNT          303 a          469 c          426 g          387 t
ORIGIN      
        1 catcacgccg ttcatgccga acctgacgag ggtgtcgctc accgacgcgt cgatccggac
       61 ccttacgaag ggggccatga tgaaaaggga gaaaaggaag aggccgatga ttatcctcgg
      121 ccagcccgcg ttttcgatga acttctttat cccggccatt gtcagaccac cttcttttct
      181 cttccgtcta tcccaagcat caggacgccg aaatccgtcg agggggcaga agccgggagt
      241 atgcccgcta tgcgacctga atcgattatc gctatccggt cacagattga gcgcagttct
      301 tccagttcgg aagagaccat cacgatcgtt gttccgaatt cgtcattgta ttttctcagg
      361 gcttccagga ccattctttt ggcgccgacg tcaatacccc tcgtcggttc cgacacgaac
      421 aggagtcgtg gttttgtctc gaaagccttg gcaaggcata ccttctgctg attgccgccc
      481 gataggtcct ggacgcgttg attctcactg aggcatttta tattgagcat atcgatgtaa
      541 tccctggcgc tcctggctat ttccgattcg tccctgatct ttatgaggcc tccgaggatc
      601 ggcttgagga accgctcctg catctggagc gacgtgaaga taatattcca tgaaatgggt
      661 tcttccatca aaaggcccac tccccgccgg tcttcataaa cggcggatat ccccgcctcg
      721 aggggactcg tggggtcgtt gagccggatc ggtccctcca ggaaaaagac tgagcccccc
      781 gcggggaaca tccccattat tccgttcgtt atccctagtt ttccctggcc ggcgaggcct
      841 ccgatcccga agatctcccg ctccctgacc tggaaagaga catccctcac cgtttccccg
      901 ggcatgtcca cccagaggtg ctccacctgg aggatgacct ccccgaggtt gccttccctg
      961 tcgccctttc ccgcaaaggc ggtggtgata tccctcccga ccatcgatgt ggctatggac
     1021 atgatatccg tctcgtccgg ggtggtgttg tcgaccacct caccatcccg gaggacgatg
     1081 atcttgtcgc agacatccat gatctcctgg agcctgtggg agatgaaaat gatggaaata
     1141 ccgagggcgg cgaggcgctt catcgtatcg aggaggatct ccgcctccga ttcggtaagg
     1201 gtcgccgtgg gctcgtcgag gacgagtatc ttggtgttct ccttgtcgat ctcgcgggct
     1261 atctcggtaa attgcttgta acccacgggg agttctgaaa cggcaatggc cgggtccagg
     1321 cctaccttca acttgtcgat cgcttcacgg ctcctgcggt ccatgtcatc cctatcgagg
     1381 gttttcatgc ggtcgccgta agcctcgacg aggaagttgt attttgtcga ttccctgttg
     1441 aggacgatat tctcggtcac tgtgaaaccg ggtatgagcg agaactcctg gtgcaccatg
     1501 cctatcccgg cttccagggc gtcgaagggg ctggcgaacc tcaccttttt cccttctatc
     1561 gagatggtgc cctcgtaccc tccgg
//
LOCUS       DAOPOA010000619         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_84657_length_1584_cov_4.667757, whole genome shotgun sequence.
ACCESSION   DAOPOA010000619 DAOPOA010000000
VERSION     DAOPOA010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84657_length_1584_cov_4.667757"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKJ17_11050"
     CDS             <1..703
                     /locus_tag="PKJ17_11050"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOD76559.1"
                     /translation="DTEKGTVSIVFMEVGASTAALARLNAGDAVATCVGPLGNATTID
                     TFGDVLLVGGCYGIGSLYPVAKELKAKGNKVSVVLEARSSYLIYWLNKYVPLAEKVYT
                     ITRDGSLGQKGHISRLPDVIHGMPRKPDRIIVNGCTYLMAHTSEVTKDLDIPVVVNLN
                     PIMIDGTGMCGVCRVTVAGQMKFACVDGPEFDGHDIDWKEFLARRKAYVTEEAMFFRR
                     SDAPAHNEGKCAWHE"
     gene            696..>1584
                     /locus_tag="PKJ17_11055"
     CDS             696..>1584
                     /locus_tag="PKJ17_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD76560.1"
                     /translation="MNEEKTQEKASKIDLNRRDMPRQSPEMRRNNFDEVALGYTEELA
                     RAEASRCLQCKKPRCVTGCPVEIDIPGFIGCITRGDFKGGIKKLKEKNCLPAVSGRVC
                     PQESQCESKCVLGNKKAEIAIGRLERFLADWEAGQGKVDLPPRAKSTGKKVAVVGSGP
                     AGLTVAGDLILLGHEVTIFEALHKAGGVLVYGIPEFRLPKAIVQREVDYLKALGVEVF
                     TDFVVGKTRSIDELLEEFDAVFVGTGAGLPWFMNIPGENLNGVYSANEYLTRMNLMKG
                     YLYPKSSTPVKKHVKVAVVG"
BASE COUNT          348 a          428 c          525 g          283 t
ORIGIN      
        1 cgacaccgag aaagggactg tctcgatagt cttcatggaa gttggggcct cgacggcggc
       61 tctcgcgcgg ctcaacgccg gggacgcggt ggcgacctgc gtgggccccc tgggcaacgc
      121 gacaaccatc gatacattcg gagatgtcct gctggtggga gggtgttacg ggatagggag
      181 cctctatccc gtggcaaagg agttgaaggc gaaggggaac aaagtgagcg tcgtgctcga
      241 ggccaggagt tcctacctga tctactggct caacaagtat gtcccgctgg ccgagaaggt
      301 ctacaccatc acgcgggacg ggagtctggg tcagaaaggc cacatcagcc gcctgcccga
      361 cgtcatacac ggcatgcccc ggaagcccga caggataatc gtcaatggat gcacctatct
      421 tatggcgcac acgtcggagg tcacgaaaga ccttgacata ccggtcgtcg ttaaccttaa
      481 tcctatcatg atcgatggca ccgggatgtg cggtgtgtgc cgggtcaccg tcgcgggcca
      541 gatgaagttc gcctgcgttg acggtcccga gttcgacgga cacgacattg actggaagga
      601 atttctcgcg cgcaggaagg cgtacgtgac ggaagaggcc atgttcttcc gcaggagcga
      661 cgcacccgcg cataacgaag ggaagtgtgc ctggcatgaa tgaagaaaag acccaagaaa
      721 aggcaagcaa gatagacctc aaccgcagag acatgccgag gcagtctccg gaaatgaggc
      781 ggaacaattt cgatgaggtg gcgctgggct acaccgagga actggcgcgg gcggaggcct
      841 cgcgctgtct ccagtgcaag aagccgcggt gcgtcacggg ttgccccgtc gagatagaca
      901 tccccggttt tatcgggtgt atcacgaggg gcgatttcaa ggggggcata aagaaactga
      961 aagagaagaa ctgcctgccc gcggtttcgg ggcgggtctg cccccaggaa tcccagtgcg
     1021 agtcgaagtg cgtcctcggc aacaagaagg cggagatcgc catcggccgc cttgaacgct
     1081 ttcttgccga ctgggaggcg gggcagggca aagtggacct gccgcccagg gcaaagtcta
     1141 caggcaagaa ggtggctgtc gtaggttccg gtccggcggg gctcaccgtc gcaggcgacc
     1201 tcatcctcct cggccatgaa gtgacaatct tcgaggcgct tcataaggcc ggcggagtcc
     1261 tcgtctacgg tatcccggag ttccgtctgc ccaaggcgat cgttcaacgg gaagtggatt
     1321 acctgaaggc cctcggggtg gaggtgttca ccgacttcgt cgtaggaaag acgaggtcca
     1381 tagacgaact gctcgaggag ttcgatgcgg tcttcgtggg gaccggggcg gggcttccct
     1441 ggttcatgaa cattcccggc gagaacctga acggcgttta ttcggcgaac gagtacctga
     1501 cgcgcatgaa cctcatgaag ggctacctct atccgaagtc gtctacaccg gtcaagaagc
     1561 atgtgaaggt cgcggtggtc ggcg
//
LOCUS       DAOPOA010000620         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_84898_length_1581_cov_2.935125, whole genome shotgun sequence.
ACCESSION   DAOPOA010000620 DAOPOA010000000
VERSION     DAOPOA010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84898_length_1581_cov_2.935125"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKJ17_11060"
     CDS             complement(<1..319)
                     /locus_tag="PKJ17_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76561.1"
                     /translation="MAKNEPVSSEAYNGMSIKIFQDESAGNPREEHSNLGCMVYWHRR
                     HVVGDVDGSKEYGRPQDFIASLPKGTVVLPVFLLDHSGLALRTESFNDPWDSGQVGYI
                     YALP"
     gene            complement(322..900)
                     /locus_tag="PKJ17_11065"
     CDS             complement(322..900)
                     /locus_tag="PKJ17_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76562.1"
                     /translation="MYDGNDGQTGCVPSTDPGIAPESVVYFADPMLTFGCASIEQEYK
                     ENVALKLELHRIKFADVRCTTDPPFAGSRRSREMGHYDILFFDWGGMGVVDQKMMSSF
                     CRLILREATDHPSRFYVVTSAFTELAMKDAMASFGDAVPANMFLSLTDFIRYYKAYAN
                     LHNKPRKPDNGQDEPGRKLRLIKGICSKKGGG"
     gene            complement(893..>1581)
                     /locus_tag="PKJ17_11070"
     CDS             complement(893..>1581)
                     /locus_tag="PKJ17_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOD76563.1"
                     /translation="EILNPPPSSPAPQQAEESGEKEAEETGEEKKEKGRNGNETEDGP
                     FMRVMDKVVHTKNMDLRAYEPEVVRKYGFDRLTDSMGTERRIYNGMIGIVANVDPDTE
                     AVYVNFPADEVYVKYDKLDARYFLQHAYALTIHKVQGSEFNVVVIPVIQAHYNMLNSK
                     LLYTAVTRAKEKAIFVGETYAFNGACKRKNDVIRNTVMRKILSGEAQQEEQDTFFGNM
                     PAYGGGAAHV"
BASE COUNT          292 a          483 c          397 g          409 t
ORIGIN      
        1 cggggagagc atagatgtac cctacttgtc ctgaatccca cggatcgtta aaagactctg
       61 tccgcagcgc caggccggaa tggtcaagga gaaagacagg gaggaccacc gttcccttcg
      121 gaagggacgc gatgaaatcc tgtggccgcc cgtattcctt gctgccgtca acgtccccga
      181 cgacatgtcg ccgatgccag tacaccatgc acccaagatt gctgtgctcc tcccgcgggt
      241 tccccgcgct ctcgtcttgg aatatcttta tggacatccc gttataggcc tcactcgaaa
      301 caggctcgtt tttagccatg tttaaccccc tccttttttc gaacatatgc ccttgatcag
      361 cctgagcttc ctgcccggtt catcctggcc gttgtcaggc tttcgcggct tgttatggag
      421 attggcgtat gccttgtagt acctgataaa atccgtgaga ctcaggaaca tgttcgcggg
      481 gacggcatcc ccgaaagatg ccatggcgtc cttcatcgcc agttccgtga aggcgctggt
      541 cactacgtag aacctcgagg ggtggtccgt cgcttctctc aaaatgagcc tgcaaaagct
      601 ggacatcatc ttttgatcaa caacacccat ccctccccaa tcgaagaaga gaatgtcgta
      661 gtgccccatc tccctggacc tgcgggatcc cgcaaaggga gggtctgtcg tgcagcggac
      721 atcggcaaat ttgatacgat gtaactccag tttcagggcg acgttctcct tgtattcctg
      781 ctctatggac gcgcacccga acgtcagcat cggatccgca aagtagacca cgctctcggg
      841 tgcgatccca ggatccgtcg acggcacgca tcctgtctgt ccatcatttc cgtcatacat
      901 gtgccgctcc tccaccatag gcaggcatat ttccgaagaa agtgtcctgt tcctcctgct
      961 gtgcctctcc ggacagtatc tttctcatga ccgtgttacg gatgacgtcg ttctttctct
     1021 tgcaggcgcc gttgaaggca tacgtttcac ccacgaagat cgccttttcc ttcgcccgcg
     1081 ttacggctgt gtagagcagc ttgctgttga gcatgttgta gtgtgcctgg atgacgggta
     1141 tgaccaccac gttgaactcg ctgccctgca ccttgtgtat cgtgagcgcg taagcatgct
     1201 ggaggaagta ccgtgcgtcg agcttgtcgt acttcacgta cacctcgtcc gcggggaagt
     1261 tcacgtagac cgcctccgta tcggggtcca cgttggctac aatgccgatc atgccattgt
     1321 agatccgtct ctccgtgccc atggaatccg tcagcctgtc aaaaccgtac ttcctcacga
     1381 cctccggttc gtacgccctg aggtccatgt tcttcgtgtg gacaaccttg tccatcaccc
     1441 tcatgaaggg gccgtcttcg gtctcgttgc cgttcctccc cttttctttt ttctcttctc
     1501 ccgtttcttc tgcttctttc tcgccgcttt cttccgcctg ctgcggcgca ggtgaagacg
     1561 gaggggggtt cagtatctcc c
//
LOCUS       DAOPOA010000621         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_85056_length_1579_cov_3.522966, whole genome shotgun sequence.
ACCESSION   DAOPOA010000621 DAOPOA010000000
VERSION     DAOPOA010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85056_length_1579_cov_3.522966"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKJ17_11075"
     CDS             <1..260
                     /locus_tag="PKJ17_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HOD76564.1"
                     /translation="DRLKDEIPRQQFKIAIQGAIGGKIISRSTISAYRKDVTAKCYGG
                     DITRKRKLLEKQKKGKKRMRIVGNVEIPQSAFLAALKTDDE"
     gene            253..1377
                     /gene="hemW"
                     /locus_tag="PKJ17_11080"
     CDS             253..1377
                     /gene="hemW"
                     /locus_tag="PKJ17_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOD76565.1"
                     /translation="MNRTGYPGLYVHVPFCKTKCPYCDFYSVPATDQAGKWLEAVLRE
                     ASLNIPTPEFTRFDSLYIGGGTPSLLGHDDIDNLIAGLRGTFRFDDGAEVTIELNPDD
                     VTKEKLALYRSLGVNRASLGVQSFDEEEVRFLGRRHSGRQAGEAVRLVAEAGFAEFGM
                     DLIYGLPGQTVRSWRKTLRQALSYGPAHLSCYQLTVEGDTPFSRLSREGGLELPDDAR
                     GAELFLAMSEYLAGHGFVQYEVSNFARGEETISRHNVKYWQHTPYLGLGPSAHSFSGR
                     RRWWNHRSLDAYLRQLGGGSSPVEDSETLSEEQMRLERLLFGFRTMWGVELQELSGKD
                     CANRLEDLREKGLVEVRGVRVVPTLRGYLFADRLPLMVSE"
BASE COUNT          337 a          451 c          465 g          326 t
ORIGIN      
        1 gcgaccgtct caaggacgag ataccgaggc agcagttcaa gatagccata cagggcgcga
       61 taggggggaa gatcatatcg cggtcgacca tctctgcata ccgcaaggac gtcacggcca
      121 agtgctacgg cggagacatc acccggaagc ggaagctcct ggaaaagcag aagaagggca
      181 agaagcggat gcgcatcgtc ggcaacgtcg agatccctca gagtgctttt ctcgccgccc
      241 tcaagaccga cgatgaatag gacggggtac cccggtcttt acgtccacgt gcctttctgc
      301 aagaccaagt gtccgtactg cgatttctac tccgtgcctg caacggacca ggccgggaaa
      361 tggcttgagg ccgttcttcg ggaggcgtcc ctcaacatcc ccaccccgga attcacccgc
      421 ttcgactcgc tctatatcgg cggcggcaca ccgtcgctcc ttggccatga tgacatcgac
      481 aacctcattg cggggctcag agggaccttt cggttcgacg acggcgccga ggtgaccatc
      541 gaactcaacc ccgacgatgt gacgaaggag aagcttgccc tgtaccgttc gcttggcgtg
      601 aaccgcgcga gcctcggcgt ccaatccttc gacgaggaag aggtgcgttt cctggggagg
      661 cgccacagcg gccggcaggc cggggaggcg gtccggcttg tggcggaggc agggtttgcc
      721 gagttcggca tggacctcat ctacgggctg cccggccaga ccgtccgctc gtggcgaaag
      781 acgctgcgcc aggccctgtc gtacggaccc gcccacctat cctgctacca gcttacagtc
      841 gagggcgaca cgcccttctc caggttgtcc cgggaggggg gtctcgagtt gcccgatgac
      901 gcgaggggag cggagctctt tcttgccatg tcagaatacc ttgccggcca cggcttcgtg
      961 caatacgagg tctccaattt cgcccgcggg gaagagacca tctcacgcca caacgtcaag
     1021 tactggcagc acacacccta tctcggtctc gggccgtccg cccattcctt ctcggggaga
     1081 cgaaggtggt ggaaccatcg cagcctcgat gcgtatctgc ggcagttggg cggaggctct
     1141 tcccccgtgg aggactcgga aacgctttca gaagagcaga tgcgcctgga acgcctcctt
     1201 ttcggtttca ggacgatgtg gggggttgag ctgcaggaat tgtccgggaa ggattgtgcc
     1261 aaccgtctgg aagatctccg agagaagggt ctggtggagg tccgtggcgt gcgtgtcgtc
     1321 ccgaccctca gaggttacct ctttgcagac aggcttccat taatggtttc cgagtagcag
     1381 tttaccagaa taattttttt attgacaaag atttcgtaat acattatcat aactcaagct
     1441 atgttaaata attcacatat ctcagacggg aatagcttga aatcagcggt atttacgatt
     1501 gcccgtaatt cagtgtgtga aactactgga aattttggtc acaacgtgca ttatttcaca
     1561 tagcatcacc taacgcgaa
//
LOCUS       DAOPOA010000622         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_85379_length_1575_cov_2.761184, whole genome shotgun sequence.
ACCESSION   DAOPOA010000622 DAOPOA010000000
VERSION     DAOPOA010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85379_length_1575_cov_2.761184"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..589)
                     /locus_tag="PKJ17_11085"
     CDS             complement(137..589)
                     /locus_tag="PKJ17_11085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD76566.1"
                     /translation="MTRAKEDDTRRDAVKGPAERSFMPEFVGFVRGMALECCYSDKTV
                     DDLGQAIEEAVSNIIRFACREKPGEIEISCTVHDTGALYITIADTGAPFNMLLAGTFP
                     EADDFFGSGERPSTKIMKRAAKNIEYKRSSNRNILIFTVSHDPGGMRR"
     gene            complement(620..1309)
                     /gene="amrB"
                     /locus_tag="PKJ17_11090"
     CDS             complement(620..1309)
                     /gene="amrB"
                     /locus_tag="PKJ17_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOD76567.1"
                     /translation="MRGGAKPLIVIFGTCHRPTDGLWSISLKDFETPLETIPCGQGLR
                     ELIGANDLLKRYVDEWPHRVEHSIELQLPLIQFMVEEDFEILPILTGSMHEYVGGLKE
                     TDDEEVKLLVENLKGVLSAYGKPCLILSGADLAHIGAQFGDRYPLDRTILESSRRKDE
                     AMLEHIRNVDAEGFFRAVKSEGDMRRICGLTPIFFQLRLLEGCTSDIVSYDQWTDGQS
                     SVSFAGGVFYR"
     gene            complement(1306..>1575)
                     /locus_tag="PKJ17_11095"
     CDS             complement(1306..>1575)
                     /locus_tag="PKJ17_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76568.1"
                     /translation="MDENLLLEGERYDRHVKGLRDEYCNAPVRKAYLAGKGYASNRME
                     LLSFLDGLFDGNAVTPAPAREIAGILAPHIDYQGAPRYTARYTHT"
BASE COUNT          298 a          468 c          463 g          346 t
ORIGIN      
        1 aagagctcag aaatgagaag gcttgtgagg ataccgggct tgcggcggaa cggagacggg
       61 gcgatcacgg ccaatggtca ggaaaggtcc ttttgtctct gaacgttcaa gcggttcaaa
      121 ctgttcaagc cgccttttac cgcctcatcc ctcccgggtc gtgggatacc gtgaagatga
      181 ggatgttcct gttggagctt cgcttgtact cgatgttctt ggcggccctt ttcatgatct
      241 ttgtcgacgg tctctcgccg gacccaaaaa agtcgtcagc ctcgggaaag gtgccggcga
      301 gcagcatgtt gaagggggcc ccggtgtcgg cgatggtgat atacagagca cccgtgtcgt
      361 ggacggtgca ggagatctcg atctccccgg gtttctctcg gcaggcaaaa cgaatgatat
      421 tgctgacggc ttcctcgatg gcctgaccga gatcgtccac ggtcttatcg ctatagcagc
      481 actccagggc cataccccgg acaaaaccca cgaattccgg catgaacgac ctttcggccg
      541 gtcctttgac cgcgtcgcgc cgcgtatcgt cctctttcgc tctcgtcatt ctcacctcga
      601 tcgttcctga cggtattgtt caccggtaga agacaccgcc ggcaaagctg accgacgact
      661 gtccgtccgt ccactgatcg tagctcacga tgtcggacgt gcacccttcg agaagtctga
      721 gctggaagaa aatgggggtg agcccgcata tcctcctcat gtccccctcg ctcttgacgg
      781 cacggaaaaa accctccgcg tcgacgttcc tgatatgctc gagcatggcc tcgtctttgc
      841 gccgcgagct ctcgaggatc gtacggtcga ggggataacg gtcgccgaac tgggccccga
      901 tatgggcgag gtcggcgccc gaaaggatca ggcagggctt cccgtaggcg gaaagcacgc
      961 ctttcaggtt ctcgacgaga agctttacct cctcatcgtc cgtctccttc aaaccgccca
     1021 cgtattcgtg catggagcct gtaaggatgg gcaggatctc gaagtcctcc tcgaccatga
     1081 actggatgag ggggagctgc agttcgatgg aatgctccac ccggtgcggc cactcatcga
     1141 catacctttt caggagatcg tttgcgccga tcagctccct caaaccctgt ccacagggta
     1201 ttgtctcgag gggcgtctcg aagtccttga gggatatgct ccagagtccg tccgtgggac
     1261 gatggcacgt gccgaagatc acgatgaggg gcttcgcgcc tcctctcagg tatgggtata
     1321 tctcgcggta taccgtggcg ccccctggta gtctatgtgc ggcgcaagga tgcccgcgat
     1381 ctcccgtgcc ggggcaggtg tcaccgcgtt tccgtcaaaa aggccgtcga ggaaggacag
     1441 aagctccatc cggttcgacg cgtatccctt ccccgcgagg tacgccttcc tgacgggggc
     1501 attgcagtac tcgtcgcgaa ggccctttac gtgcctgtcg tagcgctccc cttcgaggag
     1561 gaggttctcg tccat
//
LOCUS       DAOPOA010000623         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_85566_length_1572_cov_4.662492, whole genome shotgun sequence.
ACCESSION   DAOPOA010000623 DAOPOA010000000
VERSION     DAOPOA010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85566_length_1572_cov_4.662492"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /gene="rapZ"
                     /locus_tag="PKJ17_11100"
     CDS             complement(<1..833)
                     /gene="rapZ"
                     /locus_tag="PKJ17_11100"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOD76569.1"
                     /translation="MKDTLIVSGISGSGKTTFLRALEDAGFFCVDNFPILLLLKFLEL
                     NRDEGEKIARCAFVIDVRLRDFFENGRDIIEKAKKEFGAKVLFLESSDESLIRRYRET
                     RRSHPLFDSSNVKDALTQERKLMAWLRNMADQVIDTTHLTPHELRRLALERYGAHEQR
                     MKINIMSFGYAFGLPLEADIVLDVRFLPNPFFVEELRDQTGLDEEVREFIRSQDVFRK
                     YFLFLSEFLVYLIPLFEKEGKSYLTVCFGCTGGKHRSVFIAGELAEQLNAMGYSVSTV
                     HR"
     gene            complement(830..>1572)
                     /gene="hprK"
                     /locus_tag="PKJ17_11105"
     CDS             complement(830..>1572)
                     /gene="hprK"
                     /locus_tag="PKJ17_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOD76570.1"
                     /translation="LRSLSEAESRRIIRELCKNNVVCFIVSRNLKVPEFFVVSAEEKG
                     IPLLRTRLVTSIFIERITKLLEEKLAPSTTVHGVLMDILGVGALIMGRPGIGKSENAL
                     ELIMRGHRLVVDDAVHVKKIGAIDLYGEAPEMIKSLLEIRGVGIVDIRHLFGVSAVLD
                     KKRIDLVIELFDWDTSLECERVGLREEKFKVLSVDLPFVKIPISSGKNVSAIIELACR
                     NFILRRQGINTAVELEKRMQRSMEGGTE"
BASE COUNT          310 a          465 c          456 g          341 t
ORIGIN      
        1 cgatggacgg tggatacgct gtagcccatg gcattgagct gctcggcgag ttcgcccgcg
       61 atgaagaccg accggtgttt tccgcccgtg cagccgaagc agacagtgag ataacttttc
      121 ccttctttct cgaataacgg tataaggtac accaaaaact ccgaaagaaa aaggaaatat
      181 ttcctgaaca catcctggga tctgatgaat tccctgacct cttcatcgag cccggtctgg
      241 tcccggagct cttcgacaaa gaaggggttc ggcaggaacc tcacgtcgag gacgatgtcg
      301 gcctcgagtg gaagaccgaa ggcgtacccg aaggacatga tgttgatctt catccgctgc
      361 tcgtgggcgc cgtatctctc gagtgccagg cggcgcagtt cgtggggggt aagatgggtc
      421 gtgtctatga cctgatcggc catgttcctg agccaggcca tcagcttgcg ctcctgggtc
      481 agcgcgtcct tgacgttgga cgagtcgaag aggggatggg accgtctcgt ttcccggtat
      541 ctacgaatga ggctctcgtc ggagctttcc aggaagagga ccttcgcgcc gaactctttc
      601 ttggcctttt ctatgatatc ccggccgttc tcgaagaagt ccctcaagcg cacgtcgatg
      661 acgaaggcgc accgcgcgat cttctccccc tcatcgcggt tgagttccag gaacttgagg
      721 agcagaagga tgggaaagtt gtcgacacaa aagaaccccg cgtcctcgag ggccctgagg
      781 aacgtcgtct tccccgagcc ggatatgccg ctgacgatga gggtgtcctt cattccgtcc
      841 ctccctccat tgatctctgc atcctcttct ccagctccac ggcggtgttg atgccctgcc
      901 tgcggaggat gaaattgcgg catgccagtt cgatgatcgc cgagacgttc ttgcccgagc
      961 tgatcggaat cttgacgaag gggaggtcga cgctgagcac cttgaacttc tcttccctca
     1021 atcccacccg ctcgcactcg agggaggtgt cccagtcgaa gagctcgatg acgaggtcta
     1081 tccgcttctt gtcgaggacg gcggacacgc cgaaaagatg ccggatatcg acgatgccca
     1141 cgccccgtat ctccagcagg ctctttatca tctccggcgc ctccccgtag aggtcgatgg
     1201 ccccgatctt cttgacgtgc acggcatcat cgacgacaag gcgatggccc cgcatgatga
     1261 gctcgagggc gttctcgctc ttgccgatgc ccggccttcc catgataagg gcgccgacgc
     1321 ccagaatgtc catgaggacg ccgtggacgg tggtcgacgg ggcaagcttc tcctccagga
     1381 gcttcgttat cctctctatg aatatggacg tgacgagcct tgtccggaga agggggatgc
     1441 ccttctcttc cgctgagacg acgaagaact cggggacctt caggttccgg gagacgatga
     1501 agcacaccac gttgttcttg cagagctccc tgatgatgcg ccggctctcg gcctccgaca
     1561 gcgacctcag gt
//
LOCUS       DAOPOA010000624         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_86130_length_1565_cov_3.809934, whole genome shotgun sequence.
ACCESSION   DAOPOA010000624 DAOPOA010000000
VERSION     DAOPOA010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86130_length_1565_cov_3.809934"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="PKJ17_11110"
     CDS             complement(<1..1299)
                     /locus_tag="PKJ17_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOD76571.1"
                     /translation="MVGQWDSFLKATPQPILVLDKEFIIVAANKRSQEIAGATEGELI
                     GKRCYEVMHGTKGPAERCPMRSLLTRGNLETVEMEIETIHGTFLVSCAPIVDASGALL
                     GVVHVATDITEKKKAEEELREREVRYRYLFENSPVGMFRSSLADGSIVDANLTLSRMF
                     GIDESEDLHAEDFYADPRDRQRLLNAIKRKGVANDEVLMSRRDGSLFWALISARLDIR
                     EHYIEGVVTDITAEKEAREALRESEERYRVAIENSNDCVAIFRDGVYVYANQKYVEAL
                     GVRDAGDILGTGIGRFIHPDDYDRIERYLRERKAGKDVPTRYELRIIDVRGKVVFFEA
                     AVSVINLKGEPATLVFMRDITKRIEVQKKLEEQVEFLQNFIDTMPNPIFIKDPKGRFI
                     QCNRAFEEYYGVSRQAVEGKTVFDIAPAEEAKVHAGKDREL"
BASE COUNT          290 a          494 c          409 g          372 t
ORIGIN      
        1 cagttccctg tccttgccgg catggacctt cgcctcctcc gcgggagcga tgtcgaagac
       61 ggtcttaccc tccaccgcct ggcgcgacac accgtagtat tcctcaaagg ccctgttgca
      121 ctggatgaat cttcctttcg ggtcctttat gaagatgggg ttcggcatgg tgtcgatgaa
      181 gttctggagg aattccacct gctcctccag cttcttctgg acctctatcc gtttcgtgat
      241 gtcccgcatg aaaacaaggg tcgcaggctc acccttgagg tttatcactg aaactgccgc
      301 ctcgaagaat acgacctttc ccctcacatc gattatccgc agctcgtacc gggtgggaac
      361 atccttgccc gccttgcgct cccggagata gcgttctatg cggtcatagt cgtctggatg
      421 gatgaaccgt ccgatgcccg ttcccaggat atccccggca tcacggaccc ccaacgcctc
      481 gacgtacttt tgattggcgt agacatagac gccatccctg aatatggcaa cgcaatcatt
      541 ggagttctct atggcgacgc ggtatcgctc ctcgctctcg cgcagggcct cccgcgcctc
      601 cttctccgcg gtgatgtccg tcacgacgcc ttcgatgtaa tgctccctga tgtcgagacg
      661 ggccgaaatg agagcccaga acaagcttcc atcccgcctc gacatcagga cctcgtcgtt
      721 ggcaaccccc ttgcgcttga tcgcgttcag gagtcgctga cggtcccggg gatcggcata
      781 gaagtcctcc gcatgcaggt cctcactttc atcgatcccg aacatcctcg acagggtcag
      841 gttggcatcg acgatgcttc cgtccgccag ggagctcctg aacatgccga caggagagtt
      901 ctcaaaaaga taacggtacc tcacctccct ttccctcagc tcttcctctg ctttcttctt
      961 ctccgtgata tccgtcgcca cgtggacaac ccccaggaga gcacccgacg cgtccacgat
     1021 gggcgcgcag gagacaagga acgtcccgtg tatcgtctct atctccattt ccacggtctc
     1081 aaggttcccc cgggtgagaa gcgaacgcat ggggcatcgc tcggcgggac ctttcgtgcc
     1141 atgcatgacc tcgtagcatc ttttcccgat gagctcccct tcagtggcgc ccgctatctc
     1201 ctgcgatctc ttgttggctg cgacgatgat gaattctttg tcgaggacca ggatgggttg
     1261 gggcgtcgcc ttgaggaagc tgtcccattg tccgaccatg cgcgcaagac ttgtccccgc
     1321 ggagaatctg ttcttccctc cggcgcggac gtcagccgca gatccagatc tcgtgcggcg
     1381 cggtggcgaa gtgtcgggct gcgcaggcga cctctttttc atgctcacct gttttcatcg
     1441 tgtctgatgg tcctgatttc ttcaatgata caccattccc gtctcaggtc aagagaaacg
     1501 cgaagggctt gagtagttgg gcatggacat acttaccacc ctggggtata cccagaagaa
     1561 aaggt
//
LOCUS       DAOPOA010000625         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_86285_length_1563_cov_4.364721, whole genome shotgun sequence.
ACCESSION   DAOPOA010000625 DAOPOA010000000
VERSION     DAOPOA010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86285_length_1563_cov_4.364721"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKJ17_11115"
     CDS             <1..245
                     /locus_tag="PKJ17_11115"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOD76572.1"
                     /translation="AASAVLLLAIPAAYCYLFSPVFSDPLISGVMITMAAMPAAANTV
                     IFSEQYGADSRLASQIVFVSTIGSLVTIPLITTVLF"
     gene            634..996
                     /locus_tag="PKJ17_11120"
     CDS             634..996
                     /locus_tag="PKJ17_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76573.1"
                     /translation="MRPKLKRIEGERRTFTGTFARFGKKDRYRPKEVGGEWVAYDVTV
                     LLVDVRDGDGNPVTDHLWFNYTKGFENLGDLQEGDAIRFDARVRPYVKGYVNQREYID
                     EREVDYKLAYPTKVARVR"
     gene            complement(1236..1445)
                     /locus_tag="PKJ17_11125"
     CDS             complement(1236..1445)
                     /locus_tag="PKJ17_11125"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOD76574.1"
                     /translation="MKYRFEQCLQRGKIVRIPVDHELVAKELREAEQDLAAAEHSLRE
                     EIIKWAIIQGYYAQFHALRALVFSG"
BASE COUNT          323 a          476 c          439 g          325 t
ORIGIN      
        1 gggcagcgag cgccgtcctc ctcctcgcga tcccggcggc ctactgctac ctcttctccc
       61 cggtcttctc cgaccccctc atcagcggcg tcatgatcac gatggccgcg atgcccgccg
      121 cggcaaacac cgtcatcttc tcggagcagt acggcgccga ctcccggctt gcgtcccaga
      181 tcgtcttcgt ctcgacgatc gggtcgcttg ttacgatccc gctgatcaca acggtgctgt
      241 tctagtgctt gttgccgctt gaatctataa atctcatagg tgcactgctg cctttggcat
      301 ctttcgccct cttttgcgtg agacgttggt actccctatg accatctcgt gcgttggacg
      361 cgaagccttg acgcccgcgt atctcctccc aatcggaacc cttcgcgtct tcgcgtgaga
      421 ccagagtgcc cattccaaca tgcgacaaga gtttctaaaa taagatgaca cgatgcacta
      481 ttcagctgcc ccggctatgg cgggagcagg agggggctaa cacgttcgcc ctacttccgc
      541 aaacgatata tcggaccacc ccgattaagc ataaggcttt gcaaaggcgt aaccgtcgtt
      601 gactacgggg gttcggccgg gaacggtgag atcatgagac cgaaactgaa acggatcgag
      661 ggggagcgaa ggacattcac cggaacgttt gcgcggttcg ggaagaagga ccgttacagg
      721 ccaaaggagg tagggggcga gtgggtcgcg tacgacgtga cggtcttgct cgtcgatgtc
      781 cgggacggtg acggcaaccc ggtcaccgac catctctggt tcaattacac gaaggggttc
      841 gagaacctcg gggacctgca ggaaggggac gcgatcaggt tcgatgccag ggtcaggccc
      901 tacgtcaagg gctacgtcaa ccagagagag tatatcgatg aacgggaggt cgactacaaa
      961 ctggcctacc cgacaaaagt tgcccgggtc cggtgaaccc ccagcagcgt cgaccccttc
     1021 ccgtcattcc ttgtctcaat cgagcaatcg cccgatctga tccactacca cgctcgccga
     1081 gcatcggctt ctgagtaaac gcagccataa tccgcgcctt cccgcgtgcg catggcgaag
     1141 ttgaagtctt cgagtaccga tgccgggaga aggccttcgt cgacatagag agcctcgacg
     1201 gcataccgga ggcacgaatg gctcttctca cggtatcatc cagagaagac aagggcacgt
     1261 agggcgtgaa actgtgcgta gtagccctgg atgattgccc actttataat ctcctccctg
     1321 agagaatgct ctgcagcagc caggtcctgt tccgcttccc gcagttcctt tgcgaccagt
     1381 tcatggtcga ccgggatacg gacaatcttc ccccgctgca ggcactgttc aaaccgatat
     1441 ttcattgctc cccctcgatc aggacctttc ccgcaagaat ccgctcgtag agggccggat
     1501 cggtcgcctt cagggaccga aactctccgg gcgtgaggat gagcggcgat atctcgcggt
     1561 cga
//
LOCUS       DAOPOA010000626         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_87169_length_1551_cov_3.955214, whole genome shotgun sequence.
ACCESSION   DAOPOA010000626 DAOPOA010000000
VERSION     DAOPOA010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87169_length_1551_cov_3.955214"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKJ17_11130"
     CDS             complement(<1..397)
                     /locus_tag="PKJ17_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOD76575.1"
                     /translation="MNAIYQALADIVDAEHVSDRPEELYIYSFDLGTAEPHRPDYVVA
                     PRTTGQVREIVRLANREKVPVVPLGGGLSLAGLAVPLKGGILIDLKRMDRILEVNEKG
                     RYAVLECGVSQGQLTSYLAAHHPGLTHSEP"
     gene            complement(394..1455)
                     /locus_tag="PKJ17_11135"
     CDS             complement(394..1455)
                     /locus_tag="PKJ17_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOD76576.1"
                     /translation="MYALQFDETRCLHCESQDCLAKCQYIHIGKDGKQPGEMVRIARG
                     EDSFVLHECVTCYACEEYCPMGNHPFYLIVERQEALRISPVPRPLVTRAVNLGIPFRG
                     EPAVKEINGPLLNMAAFSELTHLIQGRLFEDLPVISTDGRKMFHYFCQLMYLHYARSS
                     VIKERLPGIIATIERHKPTEVVCFHDECYGTYASYCPAVGIDVPFTPVHFYEYLYDRL
                     TELKELIKPVGIKVAYQRPCSSRLSPDKHRFVGKIFRLIGAEEVKREFVDENALCCGG
                     TIQGQGREGSRKRAAELQRKNVEDMKKAGAEVCVFNCPACLQTLGGMVAREGIRPVHM
                     SDLCRFAIGETPAGWEVTR"
BASE COUNT          306 a          464 c          439 g          342 t
ORIGIN      
        1 caggctccga gtgcgtcaga ccggggtgat gagccgccag ataggatgtc agctgcccct
       61 gcgagacgcc gcattccagg acggcgtagc ggcctttctc gttcacctca agtatgcggt
      121 ccatgcgctt gaggtcgatc aggatcccgc ccttgagcgg gacggcgaga ccggcgagcg
      181 ataaccctcc tccgagaggg accacgggga ccttctccct gttggcaagg cgcactatct
      241 cccgcacctg tcccgtagtt cggggtgcca cgacataatc aggccggtga ggctccgctg
      301 tccccaggtc gaaggagtat atgtagagct cttccggcct gtcggaaaca tgctcggcgt
      361 ccacgatatc cgcgagtgcc tgatagatcg cgttcatcgt gtcacctccc accctgcggg
      421 tgtttcgccg atcgcgaacc ggcaaagatc gctcatgtgc acgggacgta tgccctcgcg
      481 ggcgaccatg ccaccgagcg tctgaaggca cgccgggcaa ttaaagacgc aaacctcggc
      541 ccccgccttc ttcatatctt caacgttctt cctctgaagc tcggccgcgc gcttgcggct
      601 gccttcccgt ccctgtccct gtatcgtccc gccgcagcag agggcgttct cgtcgacgaa
      661 ctccctcttc acctcttcgg ctccgatgag ccggaagatc ttgccgacaa agcggtgctt
      721 gtccggcgaa agccgtgacg aacaggggcg ctggtaggct accttgatgc cgacgggttt
      781 gatcaactcc tttaattcgg tcagacggtc ataaaggtac tcgtagaaat ggacgggagt
      841 gaagggcacg tcgatcccga cagcgggaca gtacgatgcg tatgttccgt aacattcatc
      901 gtgaaaacag acgacctccg tcggcttgtg ccgctctatg gtcgctatga tgccggggag
      961 cctctccttg atgacggaac tccgggcgta atggaggtac atgagctggc agaaatagtg
     1021 aaacatcttg cgaccgtccg tcgatattac gggaaggtcc tcgaacagcc ttccctgaat
     1081 gagatgtgtc agctcggaaa aggccgccat gttcagaagt ggcccgttta tctctttcac
     1141 ggccggttct ccccggaaag gaatgcccag gtttacggcc ctcgtaacga gcggtcgggg
     1201 aacgggagat atgcgcaagg cctcctgtct ttcgacgatg agatagaacg gatggttacc
     1261 catgggacag tattcctcgc acgcgtagca ggtcacgcac tcgtggagga cgaaggagtc
     1321 ttcaccccgg gcgatcctga ccatctcccc gggctgtttt ccgtctttcc cgatgtgtat
     1381 gtactggcat tttgccaggc agtcctggct ttcgcaatga aggcatctgg tctcatcgaa
     1441 ttgcagagca tacattagtt gttcctcccc acatctcgct gccggtcgta cagaatggcg
     1501 catttcatgc agatttcttt ctatcacaaa acgcggtcct ttttaaacac c
//
LOCUS       DAOPOA010000627         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_87187_length_1551_cov_2.864973, whole genome shotgun sequence.
ACCESSION   DAOPOA010000627 DAOPOA010000000
VERSION     DAOPOA010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87187_length_1551_cov_2.864973"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKJ17_11140"
     CDS             <1..226
                     /locus_tag="PKJ17_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76577.1"
                     /translation="VVYTPRGDRFVLFPLRVGEGDFLIDVECPGDPAMPRPDAARGRS
                     VMVSGMLVRKKLRSREMVQLKAHKIFWMEE"
     gene            239..493
                     /gene="rpsR"
                     /locus_tag="PKJ17_11145"
     CDS             239..493
                     /gene="rpsR"
                     /locus_tag="PKJ17_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOD76578.1"
                     /translation="MRTTRSDSPKKRVYVRRRVCRFCQDSNIVIDYKDPKLLKSFITE
                     RGKIMPRRMTGTCANHQRKLKEAIKMARHIAFLPFTTLLK"
     gene            506..958
                     /gene="rplI"
                     /locus_tag="PKJ17_11150"
     CDS             506..958
                     /gene="rplI"
                     /locus_tag="PKJ17_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOD76579.1"
                     /translation="MKLILIEDVQGTGKKGDTVTVKDGYGRNFLVPRGLAVPATEGNV
                     VCFENIVRSVSNKKARDLETASELKAKLEEATLTIKKKAGEDGRLFGSVTHKDIVEAV
                     KAVSGVEVDKKLVRLEEPIKTTGSHTVVIHLQPDVNAEVKIEVEKEEA"
     gene            959..>1551
                     /locus_tag="PKJ17_11155"
     CDS             959..>1551
                     /locus_tag="PKJ17_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HOD76580.1"
                     /translation="MARVLKNARDVTGPAAVRVPPQNIEVEQSLLGGLLIDPESMNKV
                     VDVVGPSDFYRDAHGKIYNLMVHLYEKNDAIDIITVSSLAKDRGLLEPIGGVTYLNTL
                     VDLMPSAANVAYYAKIVKEKSLVRTIINVATEIIEKGFNTETDIDTYIDEAEKLIFQV
                     SENKLKPTYLPVKDFVMDNIKTIERLYEKKQTVTGVPT"
BASE COUNT          407 a          413 c          444 g          287 t
ORIGIN      
        1 cgtagtctat acccccagag gggataggtt cgtacttttc ccgctgcgtg tgggtgaggg
       61 tgatttcctc atagacgtgg agtgtcccgg agaccccgcg atgccccgtc ccgacgcggc
      121 acggggccgc agcgtcatgg tatcggggat gctcgtgcgc aagaaactgc ggtcgcggga
      181 gatggtacag cttaaagcac ataaaatttt ctggatggag gaatagatgc cgagaccaat
      241 gaggacaaca cggtccgatt caccgaaaaa aagggtctat gtgagaagaa gggtgtgcag
      301 gttctgccag gattccaaca tagtcatcga ctacaaggac cccaagcttc tcaaatcatt
      361 catcacggag aggggcaaga tcatgcccag gaggatgacg gggacctgcg ccaatcacca
      421 gcgcaaactg aaagaggcca tcaagatggc ccgtcacata gcctttctgc cctttacgac
      481 tttactgaaa taacggaggc aaccgatgaa gctcatactc attgaagatg tccagggcac
      541 agggaagaag ggcgataccg tgaccgtcaa agacgggtac gggaggaact ttctcgttcc
      601 caggggactg gccgtgcccg cgactgaagg aaacgtggtc tgcttcgaga acatcgtgcg
      661 cagcgtttcg aacaagaagg cgcgggacct tgagaccgct tcggaattga aggcgaaact
      721 cgaggaagca acccttacga taaagaagaa ggccggcgaa gacggcaggc tcttcggctc
      781 cgttacccac aaggacattg tcgaggccgt taaggccgtt tcaggtgtcg aggtggacaa
      841 gaagttagtc cgcctcgagg agccgatcaa gacgaccggt tcacatacag ttgtgatcca
      901 cctgcagccg gatgtcaacg ccgaggtcaa gatcgaggtg gaaaaagaag aggcttaaat
      961 ggcccgggtc ctaaagaatg ccagggatgt gacgggccct gccgcagtgc gggtccctcc
     1021 ccagaacatc gaggtcgagc agtcattgct cggcgggctt ctcatcgacc cggaatccat
     1081 gaacaaagtg gtcgacgtcg tcggtccctc cgatttctat cgcgatgccc atgggaagat
     1141 ctacaacctc atggtccatc tctacgagaa gaacgatgcc atcgatatca tcacggtaag
     1201 cagcctcgcg aaggacaggg gcctccttga acccataggc ggtgtgacct atctcaacac
     1261 ccttgtggac ctcatgccgt ccgcggccaa tgttgcctat tacgcgaaga tcgtaaagga
     1321 gaagtccctc gtccggacca ttatcaacgt ggccacggag atcatcgaga aaggtttcaa
     1381 taccgagacc gacatcgaca cgtacatcga cgaggccgag aagctcatat tccaggtctc
     1441 ggagaacaag ctcaaaccca cctatctgcc cgtcaaagac ttcgtgatgg acaacataaa
     1501 gaccatcgag cgtctctatg agaagaagca gacggtgaca ggggtcccca c
//
LOCUS       DAOPOA010000628         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_87259_length_1550_cov_2.753846, whole genome shotgun sequence.
ACCESSION   DAOPOA010000628 DAOPOA010000000
VERSION     DAOPOA010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87259_length_1550_cov_2.753846"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKJ17_11160"
     CDS             complement(<1..325)
                     /locus_tag="PKJ17_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOD76581.1"
                     /translation="MKTDHIDTRGFITILILALLWGINYSAIKVTNTGFSPIFTSFAR
                     SVLASALGIGYCLAIRQKLLHRGPLLVHGVVVGVLFGLEFACLYLGLLFTGSARAVIL
                     IYLSPF"
     gene            492..1478
                     /locus_tag="PKJ17_11165"
     CDS             492..1478
                     /locus_tag="PKJ17_11165"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOD76582.1"
                     /translation="MKKTAIVGTGSYLPDHIMTNFDIEKFLDTSDEWIFTRTGIKERR
                     IAEKDQATSDLCKVACERAMKVAGVTASDIDLIVLATITPDTHCPAGSNWLEAKLGCT
                     KAVSFDVTAACSGFLFALHVADKFIKSGASKTALVVAGEIMSRVVNWKERESCILWGD
                     GAGAAVVTESENGAQILSTHIHTDGAAGDTLLMPGGGSKTTPISYESVDNGLHFLKMI
                     EANRSFKVAVNRFAEACEEAASVNGYTVHDADVIIPHQANLRILQGMAKKLGVPLEKV
                     YMTIEKYANISSATVPIALDEAVRDGTIKKDSLVLLTAFGGGLTWGSSLIRW"
BASE COUNT          371 a          446 c          456 g          277 t
ORIGIN      
        1 caaaaggcga caggtagatc agaatgacgg cccgcgccga gcccgtgaag agcaggccca
       61 aatacaggca ggcgaactcg agcccgaaga ggacccccac aacgacgccg tgcacgagaa
      121 gcggcccccg gtggaggagt ttctgcctga tggccaggca atacccgatc cccagcgctg
      181 aagccagaac ggaacgggcg aaggatgtaa agatgggcga gaaccccgta ttcgtgactt
      241 tgatcgccga atagttgata ccccacaaaa gggccaggat caggatggtg atgaatcccc
      301 gcgtgtcgat atggtcagtt ttcattgaga tatgacccat gttttatcga catagacggt
      361 ctatgtcaag gaaattaagc ccattaggag cgcggggacc tgggctcccg cacctgccgg
      421 gttttccctt gacaaggtgc cggaattttt gttctattta acagcaaaat caagaaaaca
      481 cggagctttc catgaaaaaa actgccatcg tcgggacggg ttcatacctg cccgatcaca
      541 tcatgacgaa tttcgatatc gaaaagttcc tggacacgtc agacgaatgg atcttcaccc
      601 gcacgggcat caaggaacgc aggatagccg agaaggacca ggccacgtcc gacctgtgca
      661 aggttgcctg cgagcgggcg atgaaggttg ccggtgtcac ggcctccgac atcgacctga
      721 tagtgctcgc caccatcaca cccgacaccc attgcccggc cgggtcgaac tggctcgagg
      781 cgaagctcgg ctgcacaaag gccgtctcct ttgacgtgac ggcggcatgc tccggttttc
      841 tcttcgccct ccacgtggcg gacaagttca taaagtcggg ggcgagtaag acggcccttg
      901 tcgttgccgg cgagatcatg agcagggtcg tcaactggaa ggaaagggaa agctgcatcc
      961 tctggggtga cggcgcgggc gctgcggtgg taacggaatc agaaaacggc gcccagatcc
     1021 tttcgaccca catccatacc gacggcgccg cgggcgacac gctcctcatg cctggcggcg
     1081 ggtcgaagac gacgccgatt tcctacgaga gcgttgacaa cggccttcac ttcctcaaga
     1141 tgatcgaggc caacaggtcc ttcaaggtgg ccgtgaaccg cttcgccgag gcgtgcgagg
     1201 aggcggcttc ggtcaacggt tacaccgtcc atgacgcgga cgttatcatc ccccaccagg
     1261 cgaatctcag gatactccag ggcatggcga agaagctcgg cgtacccctg gaaaaggtgt
     1321 acatgacgat agagaagtac gccaacattt cctcggcaac ggtccccatc gccctcgacg
     1381 aggcggtccg cgacggcaca atcaagaagg actccctcgt ccttttgacc gccttcggcg
     1441 gcggactcac ctggggaagc agcctcatcc ggtggtaacg gtttacattt gccggaaaaa
     1501 tatttagaat aatcaatccc cggagggatg gctgagcggc cgaaagcggc
//
LOCUS       DAOPOA010000629         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_87859_length_1542_cov_3.316745, whole genome shotgun sequence.
ACCESSION   DAOPOA010000629 DAOPOA010000000
VERSION     DAOPOA010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87859_length_1542_cov_3.316745"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1368)
                     /locus_tag="PKJ17_11170"
     CDS             complement(28..1368)
                     /locus_tag="PKJ17_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOD76583.1"
                     /translation="MARAQFMDHMRIRSTGHTPLWRAVCFLFILLFPATGLCITLDDA
                     IKNGLGESYVMKEQEEFVKRSRFSYISSIDPFLPQAQIGSSYIRTISPMTPISTYTGN
                     LRDTTDSRDAFTFTGSLSWRVFDGGERWAKRKGAFAVWEREKERFKSVREDVVFNVKN
                     AFYVALGAKSIVGKRQEALEAAQKIHNLTRGRYEEGAAKKSELLQAEVRFLNARIDLQ
                     KARKEYEKSLESVRSLIIVPVPDPFDVEGPLSAPTFTADYKSLNDRALATKPEIITQQ
                     REIDRLMTVYDEKKSAWYPKIDTTLQQTRQDSRFFPDGRTDAFMVSLSFPLFDGVGRY
                     YNMQGAMSDVNAARQRLAETRRTTSLDIARAVKDYELSISDVKLYAELVREATSNFNQ
                     LYGEYMEGKGDILNLLQSEKDLASANESYVSSLARAQVALAYLEKAAYLGDQRQ"
BASE COUNT          298 a          440 c          434 g          370 t
ORIGIN      
        1 ccgcgatcgc gtacaggatc tttcttttca ttggcgctgg tccccgagat aggccgcctt
       61 ttcgaggtag gccagcgcga cctgtgccct ggccagggag ctgacgtagc tttcgttggc
      121 cgaagcgagg tccttctcgc tctggagaag gttcaggata tcgcccttcc cctccatgta
      181 ttcgccgtaa agctggttga agttcgaggt cgcctcccgg acgagttccg cgtacagctt
      241 cacgtccgag atgctcaatt cgtagtcttt gaccgccctg gcaatatcga gactggtggt
      301 cctcctggtc tcggcgagcc tctggcgtgc tgcgttgacg tcgctcatgg caccttgcat
      361 gttgtagtac ctgccaaccc cgtcgaagag ggggaaggaa aggctgacca tgaaggcgtc
      421 cgtcctgccg tcaggaaaga accggctgtc ctgcctggtc tgctgaaggg tggtgtctat
      481 tttcggatac caggcgctct tcttctcgtc gtagaccgtc atcaacctgt ctatctctct
      541 ttgctgggtt atgatctcgg gctttgttgc gagggccctg tcgttcaggc tcttgtaatc
      601 ggccgtgaac gtgggggccg aaagcggacc ctcgacatcg aaagggtccg gcaccgggac
      661 gatgatgagc gacctcaccg attccagcga tttttcgtac tccttcctgg ccttttgcag
      721 atcgatcctg gcgttgagga atcggacctc cgcctgaagc agttcacttt tcttcgcagc
      781 cccttcctcg tatctccctc gagtaaggtt gtgtatcttc tgcgccgcct ccagtgcttc
      841 ctgcctcttt ccgacgatgg actttgcccc aagcgcgaca tagaacgcgt tcttgacgtt
      901 gaatacgaca tcttcccgga cgctcttgaa acgctccttc tccctttccc agaccgcgaa
      961 ggcacccttt ctcttcgccc agcgctcgcc gccgtcgaag acccgccagg agagcgagcc
     1021 ggtgaacgtg aaggcgtcac gggaatcggt ggtgtccctc aggttgcccg tataggtgct
     1081 tatcggcgtc atgggagata tcgtccgtat gtacgaggac ccgatctggg cctgcggcag
     1141 aaagggatct atggacgata tgtaggaaaa ccgcgacctc ttgacgaact cctcctgctc
     1201 cttcatgacg taggattcgc ccagaccgtt ctttattgcg tcgtcaaggg tgatgcaaag
     1261 acccgtcgcg ggaaagagga gaatgaagag gaagcagacg gcccgccata gcggggtatg
     1321 tcctgtcgaa cgaattctca tgtggtccat gaactgagct cgcgccatct tatccgcgga
     1381 aacgtcttta aaactgccgc aagtgctatt gtagcacaat cattcttctg tcacaatcga
     1441 aaggcatggg gtcaggctcg aaaagattgg ggaacaagga agaagaaaat gccgaggaac
     1501 ggaattgaac cgctgacacg gggattttca gtcccctgct ct
//
LOCUS       DAOPOA010000630         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_87861_length_1542_cov_3.305985, whole genome shotgun sequence.
ACCESSION   DAOPOA010000630 DAOPOA010000000
VERSION     DAOPOA010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87861_length_1542_cov_3.305985"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKJ17_11175"
     CDS             <1..576
                     /locus_tag="PKJ17_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD76584.1"
                     /translation="AVVDDAHGTGIFGRNGSGVEEHWGLKGKADIHMATFGKAMGSFG
                     AFVLSDPVVIEYLINRARTFMYTTALPPATLAASQAALELVAGDLSFKEELWRNIDHM
                     RSGLLSAGFDLKQSVGPIVPIVVGRDADAVKMQKMLMRKGFFLQAIRPPTVPTGTSRL
                     RLTVVRGFTREQMDSAIEAISEAGRKMKLVP"
     gene            970..>1542
                     /locus_tag="PKJ17_11180"
     CDS             970..>1542
                     /locus_tag="PKJ17_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOD76585.1"
                     /translation="MYSSEQLKDWDKRYIWHPFTQMGDYMRSDPLVIERGEGFHLIDT
                     EGRRYIDGVSSLWVLVHGHGRKELTDAMERQSKELCHSTMLGLANVPAIVLAKRLAEI
                     TPAGLEKVFYSDNGSTSVEIAIKMSYQYWQQKGEKKKKRFISFLNGYHGDTIGSVSVG
                     GIDLFHKVYRPLLFRSYKSPSPYCYRCPMKL"
BASE COUNT          348 a          420 c          471 g          303 t
ORIGIN      
        1 gccgttgtcg acgatgccca tgggacgggc atattcggca ggaatggaag cggtgtcgaa
       61 gagcactggg ggctgaaagg gaaggccgat atccacatgg ccaccttcgg aaaggccatg
      121 gggtccttcg gcgccttcgt cctctccgac cccgtcgtca tagaatacct gatcaacagg
      181 gcacggacct tcatgtacac gacggccctg cctccggcga cactcgcggc gtcgcaggcg
      241 gccctcgaac tcgtggcagg ggacctgtcc ttcaaagagg aactctggcg caacatcgac
      301 cacatgcgct cgggactgct gtcggcgggt ttcgacctca agcagagcgt gggccccatt
      361 gtccccatcg tggtgggcag ggacgccgac gccgtgaaga tgcagaagat gctcatgagg
      421 aagggtttct tcctccaggc catccgcccc cccaccgtcc ccacgggcac atcgaggctg
      481 cgccttaccg tggtccgggg cttcacgcgg gagcagatgg acagcgccat cgaagcgata
      541 agcgaggcgg gaaggaagat gaaactggtc ccctgagggg accgcttgaa tggcttgaat
      601 cgcttgaatc gcttgagagt gaaggcaaag aggctgaatg cggctgaatg ccggaatatg
      661 gaagataagg tttttggccc tcaacgggat atcgacaaag ctggttttcg agttcgactc
      721 gtgttatatc gtcattccgg cgaaggccgg aatccaggaa atatgaggga gcaggaaaac
      781 ctccggatcc cggatcgagt ccgggatgac gacgatgagg actttatcga cgacccgttg
      841 aaggtttaag acaggagaag gcttgaatgg cttgatcggt tgctgatagg tgtagatgtc
      901 cttgagagat aaggtttcca gccctcaagc gactcaagcc gttgttttga tccgaaggag
      961 caaaagacca tgtacagcag cgagcagttg aaggattggg ataagcgtta catctggcac
     1021 ccctttaccc agatggggga ttacatgaga agcgatccgc tcgtcatcga gcgcggtgaa
     1081 ggcttccatc tcatagatac cgaggggagg cgctacattg acggcgtttc ctcgttgtgg
     1141 gtcctcgtcc acggacacgg ccgaaaagag cttacggatg ccatggagag gcaatcgaag
     1201 gagctttgcc actcgaccat gctcggtctt gccaacgttc ccgccattgt cctggcgaag
     1261 cggctcgcgg agatcacgcc ggcaggcctc gaaaaggtgt tctattccga caacggctcc
     1321 acgtcggtgg agatcgccat caagatgtcc tatcagtact ggcaacagaa gggggagaag
     1381 aaaaagaagc gcttcatatc cttcctgaac ggctaccacg gcgacaccat aggctccgtg
     1441 agcgtgggcg gcatcgacct ctttcacaag gtctacaggc cgctcctttt ccggtcgtac
     1501 aagtcgccgt cgccctactg ctaccgttgt cccatgaagc tc
//
LOCUS       DAOPOA010000631         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_88243_length_1537_cov_2.748988, whole genome shotgun sequence.
ACCESSION   DAOPOA010000631 DAOPOA010000000
VERSION     DAOPOA010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88243_length_1537_cov_2.748988"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKJ17_11185"
     CDS             <1..418
                     /locus_tag="PKJ17_11185"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOD76586.1"
                     /translation="RLNRIEYQVKIRRGEIRAIEALRQEIASIETDMNRIREFKESTP
                     MTLDVMKELTTVLPKSVWLTRLRITGETVETEGYAGSATEMLPKLEQSSLFKKVEFSS
                     PTIRDTRQNADRFVIKMQMEGFEEKKTGGPKDERKK"
     gene            402..956
                     /gene="gspM"
                     /locus_tag="PKJ17_11190"
     CDS             402..956
                     /gene="gspM"
                     /locus_tag="PKJ17_11190"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOD76587.1"
                     /translation="MKGRSKLVSVAVPVIVVLAGLVAYQYGYLAVKAERASLRERQAA
                     RWATLERYVNLIAERPLLEKKLESLKERRNAETAKVISAETLSIAAATLQNIVKGSVT
                     GRGGSIASERVERPEDLGKFKVVSVSVDAVLPDTRALGDVLYTLETNVPYLVIKEVDT
                     RVRNFRDPRDLMVKLRVSALASGK"
     gene            1093..>1537
                     /locus_tag="PKJ17_11195"
     CDS             1093..>1537
                     /locus_tag="PKJ17_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76588.1"
                     /translation="MKALRYLIHNATVTNMILLAVVMAVAAATVIPLIRTRHAYSLPG
                     IKTKPAGEEAQREELPPKLPSDYAVIGETNLFHPDRVIPVDRKAEVPRPEVVLYGTMI
                     DTTRLAFIEDRKNPVTTPGRGKRQRVVKLGEEVSGYTVAEIGTDRI"
BASE COUNT          374 a          410 c          499 g          254 t
ORIGIN      
        1 gaggctcaat cgcatcgaat accaggtgaa gatacgcagg ggcgagatcc gggcgatcga
       61 agcattgagg caggagatcg cgtccatcga gacggacatg aacaggatca gggaattcaa
      121 ggaatccacc cccatgactc tggacgttat gaaggaactg accacggtcc tgccgaaaag
      181 cgtctggctc acgcggttga ggataacggg cgagacggtg gagaccgagg gatacgccgg
      241 ctcggcgacg gagatgctcc cgaagcttga gcaatcgagc ctcttcaaga aggttgagtt
      301 ctcttcaccc accatacggg acacgcggca gaacgccgac cgcttcgtca tcaagatgca
      361 gatggaaggt ttcgaggaga agaagacggg agggccgaaa gatgaaagga agaagtaagc
      421 tcgtctccgt tgccgttccg gtcatcgtgg tgcttgccgg tctcgtcgcg taccagtatg
      481 ggtacctggc ggtcaaggcc gaaagagcat cgctcaggga gagacaagcg gcgcgatggg
      541 caaccctgga gcgatacgtg aacctcatcg ccgagaggcc tttgctggaa aagaagctgg
      601 agtccctgaa ggaaaggagg aatgccgaga ccgccaaagt gatctcggcg gagaccctca
      661 gcattgccgc ggcaacgctc cagaacatcg tgaagggatc cgtaacggga agaggaggat
      721 ccatcgcgag cgaacgcgtc gaaagaccgg aagacctggg gaagttcaag gtggtcagcg
      781 tgagcgtcga cgcggtactg ccggatacca gggccctcgg cgatgttctg tatacgctgg
      841 agacgaacgt gccatacctt gtgatcaagg aagtggacac ccgtgtcagg aatttcagag
      901 accccaggga cctgatggtc aagcttcggg tgtcggccct ggcgagcggc aagtgatgtg
      961 aaagccctca ggtatctcat ccacaacacc accatgtaat caaggaagtg gacacccgtg
     1021 tcaggaattt ctgagacccc ggggacctga cggtcacact ccgggtatcg gtccccgcgg
     1081 gaggtaaatg acatgaaagc tctaaggtac cttatccaca atgccaccgt gacaaacatg
     1141 attctcctcg ccgttgtcat ggccgttgca gccgcgacgg tcatacctct catcaggacg
     1201 cgccatgcgt attctctgcc ggggatcaag acgaagccgg ctggtgagga ggcgcagcgg
     1261 gaggagcttc cgccgaagct gccttcggac tacgcggtca taggggagac gaacctgttc
     1321 cacccggacc gggtcatccc cgttgacagg aaagcggagg ttcccaggcc ggaggtcgtg
     1381 ctctatggca cgatgatcga cacgacccgc ctcgccttca tagaggaccg gaagaatccc
     1441 gtgacgactc ccggcagggg caagcgccag cgggtcgtga agttgggaga ggaggtgagc
     1501 gggtataccg tcgccgagat cggaaccgac aggatag
//
LOCUS       DAOPOA010000632         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_88547_length_1533_cov_3.598106, whole genome shotgun sequence.
ACCESSION   DAOPOA010000632 DAOPOA010000000
VERSION     DAOPOA010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88547_length_1533_cov_3.598106"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKJ17_11200"
     CDS             <1..478
                     /locus_tag="PKJ17_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76589.1"
                     /translation="ACIFVAVCMLLIQPGCTGEGEKSPGPQDAKTEPQTRQAKAGGFT
                     VPEEGTPAERVRKLWESTGPIMKVDAQANLIDREYQNKRTPLFSVWVTLQGKLSLAPQ
                     KDERAQRVIPEVLKLIDDLYGFPGFTEERREKDRETAKKFFDKRYGRLEEEIKALK"
     gene            complement(656..1060)
                     /locus_tag="PKJ17_11205"
     CDS             complement(656..1060)
                     /locus_tag="PKJ17_11205"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOD76590.1"
                     /translation="MLSLMLALLAVLCISACAEDQRTAGVPPEKVREFTDPSRTIQVT
                     AGETFSIVLDSNPTTGYGWQRQDESGNGVVAFRGSRFVAPPKNLVGAGGREHITFSAT
                     AQGTQKLTFHYLRPWERNTEPAKTIVFTVLVR"
     gene            complement(1131..>1533)
                     /locus_tag="PKJ17_11210"
     CDS             complement(1131..>1533)
                     /locus_tag="PKJ17_11210"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOD76591.1"
                     /translation="YAEATVPKVTVITRKAYGGAYDVMSSKHLRGDVNFAWPTAEIAV
                     MGAKGAVEIIFRAEAAGDPEKLAAKTEPKDFPDRNPHKSHLGTINCTVCHKAHAASKT
                     YCLECHKNFNMKIPFGEDQAGTGATPNKKAQ"
BASE COUNT          307 a          425 c          464 g          337 t
ORIGIN      
        1 cgcttgcatc tttgtcgccg tctgcatgct cctcatccag ccggggtgta ccggcgaggg
       61 agagaaatcc ccggggcccc aggacgcaaa aactgagccg cagacccgcc aggcgaaagc
      121 gggaggcttc accgttcccg aggaagggac gccggctgaa agggtcagga aactctggga
      181 atctacgggt cccatcatga aggtggacgc gcaggcgaac cttatcgaca gggagtacca
      241 gaacaagagg acgcccctct tttcggtctg ggtcacgctg caggggaaac tctccctggc
      301 ccctcagaaa gatgagaggg cgcagcgggt tatcccggag gtcctcaagc tcatagacga
      361 tctctatggt ttcccgggat tcaccgagga acgccgcgaa aaggaccgcg aaacagcaaa
      421 gaagttcttc gacaagcgat acggcaggct ggaggaagag ataaaagcat tgaaatgagc
      481 tgcttgaacc gcttgaacag cttaagctgc ttgaacgttg aaaacaaaag accataggga
      541 aggagtacgg cctgactccc ggttgtgatc cggggggcga attgtgtctg tctctttgtt
      601 tttagggacc gcaggggttc ttgcgctcaa gcggcttaag ctgttcaagc ggtcttcacc
      661 gtaccagtac ggtgaagaca attgtttttg ccggttcggt gttcctttcc caggggcgga
      721 ggtagtgaaa ggtcaacttt tgcgttccct gtgcggtggc actgaaggtt atgtgctccc
      781 tgccgccggc accaacgagg ttcttcggag gggcgacaaa cctgctgcct ctgaaggcaa
      841 ccacaccgtt tccgctctcg tcttgccttt gccagccata acccgttgtc ggattcgagt
      901 cgaggacgat ggagaatgtc tcgcccgccg tgacctgtat cgttcgagaa ggatccgtga
      961 attccctgac cttttccggc ggcacgcccg ccgttctctg atcttccgca catgctgaga
     1021 tgcacagaac cgccagaagg gcgagcatca gcgagagcat tctggtcact gttcatcctc
     1081 ccgcgatcgt ctcacggtga cgttaccaga tgacgcttcc ggaaacctcc tcactgtgct
     1141 ttcttgttcg gggtcgcccc ggttcccgcc tgatcttcgc cgaagggtat cttcatattg
     1201 aaattcttgt gacactcgag gcagtaggtc ttcgatgccg cgtgggcctt gtggcacacg
     1261 gtacagttga tggtcccgag atgggactta tgggggttcc tgtcaggaaa atctttgggt
     1321 tctgtcttcg cggcgagctt ttccggatcg ccggcagctt cggcgcggaa gatgatctcg
     1381 acggcgccct tggcgcccat gaccgcgatt tcggcggtcg gccaggcgaa gttcacgtcg
     1441 ccgcgcaggt gcttcgagct catgacgtca taggcgccgc cataggcctt ccgggtgatg
     1501 acggtgactt tcggaaccgt ggcttcggca tag
//
LOCUS       DAOPOA010000633         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_88561_length_1533_cov_2.820704, whole genome shotgun sequence.
ACCESSION   DAOPOA010000633 DAOPOA010000000
VERSION     DAOPOA010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88561_length_1533_cov_2.820704"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /gene="nth"
                     /locus_tag="PKJ17_11215"
     CDS             <1..779
                     /gene="nth"
                     /locus_tag="PKJ17_11215"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOD76592.1"
                     /translation="RRSTGRAHTRKKCVSGSRSSSTVPPGEPTDPWRYKMKPVKKGFP
                     FDEMMKTLIDLFPEEAAPIITRISRRETSDPFVVLVGTLLSLRTKDETTEKVMETLAP
                     RAATPRALLDIPDGELERMIYPVGFYRNKAKVLKEVARTIIDRYDGKVPDTIEELLSM
                     RGIGRKTANLVVTEGYGKPGICVDTHVHRISNRLGIVSTKNPHATEDALRQVLPGQYW
                     IVYNSLLVTFGKRICTPLSPKCSECPIEHLCPRKGVTRSR"
     gene            888..>1533
                     /locus_tag="PKJ17_11220"
     CDS             888..>1533
                     /locus_tag="PKJ17_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76593.1"
                     /translation="MKKILFYTHNIFTKENPVGYRIQQYFPYLEEKGFKVSLLTTKAD
                     LGTVLKTASRSDVVVVQRVLLSPMKLSLLKMFAKKMVYDFDDAVMYGSKGESATRRSR
                     FAAIVKASKAVLCGNSFLMEEAGRYRDSGIHYMPTTVDIDEYPVKVHEERKTFVVGWI
                     GTSSTLRYLDGIRDLIISITDREGVEFRVIADKPFGVEKRGITFQRWNKEREKSM"
BASE COUNT          369 a          444 c          452 g          268 t
ORIGIN      
        1 caagaaggtc tactgggagg gctcacacgc ggaagaaatg cgtgagtggc tcgagaagct
       61 catcgacggt gccgcccggt gagccgacgg acccatggag atacaagatg aagccggtga
      121 aaaaaggatt cccctttgac gagatgatga agaccctcat cgatctcttc cccgaggagg
      181 cggcgccgat catcacccgc atatcccgaa gggagacgtc agaccccttc gtcgtgctcg
      241 tcgggacact cctcagcctt cggacgaagg acgagacgac ggagaaggtg atggagaccc
      301 tcgcaccccg cgccgcgacc ccccgggcac tccttgacat ccccgatgga gagctggaga
      361 ggatgatcta tcccgtgggt ttctaccgga acaaggcaaa ggtcctcaag gaggtcgccc
      421 ggaccatcat cgacaggtac gatggaaagg tccccgacac catcgaggag cttctctcca
      481 tgcgggggat aggccgaaag acggctaacc ttgtcgtcac cgaagggtac gggaagccgg
      541 gcatatgcgt ggatacgcac gtccacagga tatccaacag gctcggcatc gtctcgacga
      601 agaaccctca cgccaccgag gatgccctga gacaggtact gcccgggcag tactggatcg
      661 tctacaactc cctgctcgtg accttcggca agcggatatg cactcccctg tcgccgaaat
      721 gcagcgagtg tcccatcgaa cacctctgcc ccaggaaagg ggtaacgagg tcgagataag
      781 cggacggatg ccatgaccat ccgctccggg tcctccccgg cgttgacagc ccgatacggc
      841 gatgatatat tcaacatgtc tttaagggcg atggcgcgcc gtactccatg aaaaagatac
      901 ttttttacac ccacaacata ttcacgaagg aaaaccctgt gggatacagg atccagcagt
      961 atttcccgta tctcgaggaa aagggtttca aggtctccct gctgacgaca aaggcggacc
     1021 tcggcaccgt cctcaagacg gcctcgcgat ccgacgtcgt cgtcgtgcag agggtccttc
     1081 tgagccccat gaagctctcc ctcctcaaga tgttcgcgaa gaagatggtc tacgatttcg
     1141 atgacgccgt catgtacggc agcaagggtg agagcgcaac gagaaggagc aggttcgccg
     1201 ccatcgtcaa ggcctcgaag gcggtcctct gcgggaactc ctttctcatg gaggaggccg
     1261 gcaggtacag ggattcgggc atccactaca tgcccaccac cgtggacatc gacgaatacc
     1321 ccgtgaaagt gcacgaggag cgcaagacct tcgtggtcgg atggataggg acgagttcca
     1381 ccctgcggta cctcgacggc atcagggatc ttatcatttc catcaccgac agggaggggg
     1441 tcgagttcag ggtcatcgcg gacaaaccct tcggtgtcga gaagcggggg ataacctttc
     1501 aaagatggaa caaggagcgg gaaaagtcga tgc
//
LOCUS       DAOPOA010000634         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_88637_length_1532_cov_3.079892, whole genome shotgun sequence.
ACCESSION   DAOPOA010000634 DAOPOA010000000
VERSION     DAOPOA010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88637_length_1532_cov_3.079892"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /gene="tgt"
                     /locus_tag="PKJ17_11225"
     CDS             <1..729
                     /gene="tgt"
                     /locus_tag="PKJ17_11225"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOD76594.1"
                     /translation="DIAMCLDECVSYPSTFEYTSESVELTTRWARRCLAARSEEGTQK
                     LFGIIQGGFYPDLRLRSAEDILSLDFDGFAIGGLSVGEPKSLMWDMVDTVVGLMPAGK
                     PRYMMGIGLPEDLLEGVRRGIDMFDCIIPTRFARNGSLFTWDGRINIKNARFTEDGGP
                     IQEGCTCYTCRTFSRAYLRHLTVSHELTSFYLNTIHNVHFYMELMKRIRSAVSAGTFD
                     AFYHDFKRRYEGGESDDRYSVCNG"
     gene            701..1021
                     /gene="yajC"
                     /locus_tag="PKJ17_11230"
     CDS             701..1021
                     /gene="yajC"
                     /locus_tag="PKJ17_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOD76595.1"
                     /translation="MTGIAYAMGSNPAGGAGQGGGSYMSLIFIAAVFAIFYFILIRPQ
                     QKRAKQHKDFLENLKKGDKVITSGGLYGTITGISDDSVTIEIAEKVRVKVDKGTVVQY
                     QKTQ"
     gene            1018..1386
                     /gene="rpsF"
                     /locus_tag="PKJ17_11235"
     CDS             1018..1386
                     /gene="rpsF"
                     /locus_tag="PKJ17_11235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOD76596.1"
                     /translation="MKGDVNVGRYENIVVLSPDLAKEEEDDLMKRIQGNLERAGATIV
                     KLDDWAVRKLAYPIKKKDKGHYFFYLLDMDEGSVATMAKFYRTLDPVLRHMFVVVDDK
                     SEGPKKPPDPVVFDELEGEF"
BASE COUNT          360 a          390 c          459 g          323 t
ORIGIN      
        1 gacatagcaa tgtgcctcga cgaatgcgtt tcctatccct ccaccttcga atataccagc
       61 gaatccgttg agttgacgac gcggtgggcg cggcggtgcc tcgcggcccg gtcagaagag
      121 gggacgcaaa agctcttcgg gatcatccag ggcggcttct atcccgatct caggttgcgt
      181 tcagcggagg acatcctttc cctcgatttc gacggttttg ccatcggggg tctcagcgtc
      241 ggggaaccga agagcctgat gtgggacatg gtggacacgg ttgtcggcct tatgcccgcg
      301 ggaaaacccc gctacatgat gggcatcggc cttcctgaag acctcctgga aggtgtgagg
      361 cggggcatag acatgttcga ctgcatcatt cccacgcgtt tcgcccgcaa cggcagcctc
      421 ttcacatggg acggcaggat caacatcaag aacgcccgct tcaccgagga tggggggccc
      481 atccaggaag gatgcacctg ttatacctgc aggacctttt cgagggccta tctcaggcac
      541 ctcacggtct cgcacgagct gaccagtttc tacctgaaca ccatacacaa tgtccatttc
      601 tacatggagc ttatgaagag gatccggagc gcagtcagcg ccggcacgtt cgacgctttt
      661 tatcacgatt tcaagagacg ttacgaagga ggtgaatcag atgacaggta tagcgtatgc
      721 aatgggtagc aatcccgccg gcggggccgg acaggggggc ggttcgtaca tgtcgctcat
      781 ctttatcgcg gcggtcttcg ccatctttta cttcattctc atccgtccgc agcagaaaag
      841 ggcgaagcag cacaaggact tcctggagaa cctgaagaag ggagataaag ttatcacatc
      901 gggtggttta tatggtacaa taacgggtat ttccgatgac agtgtcacca ttgagatcgc
      961 cgagaaagtg cgtgttaaag tcgacaaggg cactgtggtg cagtatcaga agacacaatg
     1021 aaaggagatg tgaacgtggg aagatatgag aatattgttg tgctcagccc tgacctcgcg
     1081 aaagaggaag aagacgatct catgaagagg atccagggca accttgaaag ggcgggcgca
     1141 acgatcgtca aactggacga ctgggcggtg agaaagctcg cctatccgat aaagaagaag
     1201 gacaagggcc attacttctt ctacctgctt gatatggacg aaggcagcgt cgcgacgatg
     1261 gccaagttct acaggaccct cgaccccgtg ctcaggcaca tgttcgttgt cgttgacgac
     1321 aagagcgagg gaccgaagaa accgcctgat cccgttgtgt ttgacgagct cgaaggcgag
     1381 ttttgatgaa cagggtcttt atgacgggga aggtcgaggc catgccccgt gtcgtctata
     1441 ccccgaaggg ggacaggttc gtacttttcc cgctgcgcgt gggtgagggt gatttcctca
     1501 tagacgtgga gtgtcccgga gaccccgcga tg
//
LOCUS       DAOPOA010000635         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_88922_length_1529_cov_1.734057, whole genome shotgun sequence.
ACCESSION   DAOPOA010000635 DAOPOA010000000
VERSION     DAOPOA010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88922_length_1529_cov_1.734057"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1529
                     /locus_tag="PKJ17_11240"
     CDS             <1..>1529
                     /locus_tag="PKJ17_11240"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD76597.1"
                     /translation="RHGLEVSFPSTVAADAPNTRKDPSAQHPAQDADTGRSSAQQEDE
                     DGRKHPIRAEIDRVIAMQREGDFEGARKALEPLLTGEPGNADVRYLNARLFMADGDMK
                     RAIDELAETVRLDPGHSNALNDLGCMAFQQEKYPEAFESLKRAIVSDRTNYMALQNML
                     ALLGRLRSQEPSLDAVGILRELYPEGFRDIGAGDAAATKRALVIYADEAIPWALGNML
                     EHFPYLNGHSMWWETAEMVKILNQRGYTVDYIGLPVQHLVDTHWDWDRYHLVIDGGNN
                     NLLKCPPSQRPVKVFYSTGKHWLLANVAEAQRNLMFYRRHGVHMPLERTQRNNFSDEN
                     ADYLTYFGNESQLEGYSDNPRKVPLNISSACIPLYREKDVSRAKKSYIWLGGQAMVHK
                     GLDLTVEAFARMPEFSLHICANLEIEPRFLKWLTGYLRKCPNIRYHGPQNVASAGFQE
                     LAWNSVGTVYVSAAEGGPGSVAQLLHFGIIPIVTRTSNVRAEHLGYVIEHTSDNTIID
                     G"
BASE COUNT          351 a          470 c          438 g          270 t
ORIGIN      
        1 cgtcacgggc tggaggtctc gtttccctcg acggtcgcag cggatgctcc aaatacccgg
       61 aaagaccctt ccgcccaaca ccccgcccag gacgcggaca ccggccgatc atcggctcaa
      121 caggaagatg aggacggaag aaaacacccg atccgcgccg agatcgacag ggtcatcgcc
      181 atgcagcggg aaggggattt cgaaggagca cggaaagcgc tggagccgct cctgacaggg
      241 gagcccggca acgccgacgt gcgctacctg aatgcccggt tgttcatggc agacggcgac
      301 atgaaaaggg ccatagacga acttgccgaa acggtccggc tcgatcccgg tcactccaac
      361 gccctcaacg atctcgggtg catggccttt caacaggaga agtatccgga ggcctttgaa
      421 agcctgaagc gggccatcgt ttcagacagg accaactaca tggcgcttca gaacatgctt
      481 gccctcctcg gccgtcttcg ttcccaggaa ccgtccctcg atgccgtggg tatcctgagg
      541 gagctctacc ccgagggttt cagggacata ggggcaggcg atgcagcggc aacgaaacgg
      601 gcgctcgtca tttacgccga tgaggcgatc ccctgggcgc tggggaacat gctcgagcac
      661 tttccctatc tcaacggcca ctcgatgtgg tgggagacgg ccgagatggt aaaaatcctc
      721 aaccagcgtg gatatacggt agactacatc ggcctgcccg tccagcacct cgtggacacc
      781 cattgggact gggacaggta ccatctggta atagacggag gcaacaataa cctcctcaag
      841 tgtcctcctt cgcaacgccc ggtgaaggtc ttctattcga ccggcaagca ctggctcctt
      901 gccaacgttg ccgaggcgca gcgcaatctc atgttctaca gacgccacgg ggtccacatg
      961 cccctggaac gaacgcagag gaacaatttc tcggacgaaa acgcggatta cctcacctat
     1021 ttcggcaacg aatcacagct tgagggttat agtgacaacc ccaggaaggt gccgctcaac
     1081 ataagctccg cgtgtatccc gctctaccgg gagaaggatg tctcgcgggc aaagaaaagc
     1141 tatatctggc tcggcggaca ggccatggtc cacaaggggc tcgacctgac ggtcgaagcc
     1201 tttgcgcgaa tgcccgaatt cagcctgcac atctgcgcca atctcgagat cgagccgcga
     1261 ttcctcaagt ggctcacggg ttatctgcgg aagtgcccca acatcaggta tcacgggccg
     1321 cagaatgtcg cttcagcggg tttccaggaa ctcgcctgga actcggtggg aacggtctat
     1381 gtcagcgcgg ccgaaggcgg gccgggctcg gtggcacagc ttctccactt cggcatcatt
     1441 cccatcgtca ccaggaccag caatgtccgg gccgaacacc ttggatacgt catcgaacac
     1501 acctccgaca acacaattat cgacgggat
//
LOCUS       DAOPOA010000636         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_89100_length_1526_cov_3.252889, whole genome shotgun sequence.
ACCESSION   DAOPOA010000636 DAOPOA010000000
VERSION     DAOPOA010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89100_length_1526_cov_3.252889"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKJ17_11245"
     CDS             complement(<1..377)
                     /locus_tag="PKJ17_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76598.1"
                     /translation="MKKSGAHMNRLVIKIGTSVLLDDEKKISTGRIRDIARQVRKVKE
                     KGIDVIIVSSGAIGCGMETLGLARKPKEMAKRQALASMGQVLLMKMYRENFEKKGMKV
                     SQILLTHEDIKNRTRCLNLTNTL"
     gene            complement(381..1391)
                     /gene="obgE"
                     /locus_tag="PKJ17_11250"
     CDS             complement(381..1391)
                     /gene="obgE"
                     /locus_tag="PKJ17_11250"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOD76599.1"
                     /translation="MKFIDEAIIYVESGKGGSGCVSFRREKFIPRGGPDGGDGGKGGD
                     VIIAGKKDLASLHDFRYKRNYKAENGKAGAGKNKTGREGRDVTIHVPLGTVVYDRDTS
                     ELLFQVLRDGETHAAALGGRGGRGNTRFVTSTHRAPLEFDAGEEGQRRWLHLDLKLLA
                     DVGLVGHPNAGKSTLISRLTQARPKVGDYPFTTLTPALGVLDDNEKTFVIADIPGIIE
                     GASKGKGLGLAFLKHIERTNALLIVLDLSSDDVKGDYRTLLEELGSYNEAMLQKRRLV
                     ILNKADLVTPDVAQRWRSYLKRKGEKAVVVSALTLAGVDELVSAISEGVEALRQNLNQ
                     AV"
     gene            complement(1391..>1526)
                     /locus_tag="PKJ17_11255"
     CDS             complement(1391..>1526)
                     /locus_tag="PKJ17_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOD76600.1"
                     /translation="QHGTKIHPGQNVGIGKDDTLFALIDGIVKFDMAGDRRRAHVVPV
                     "
BASE COUNT          258 a          479 c          415 g          374 t
ORIGIN      
        1 tcaagggtat tcgtcaggtt gaggcagcgt gtcctgttct ttatgtcttc atgggtgagc
       61 agtatctggc tcaccttcat gcccttcttc tcgaagttct cgcggtacat cttcatgagg
      121 agcacctggc ccatcgatgc cagtgcctgc cgcttcgcca tctccttcgg cttgcgggcg
      181 aggccgaggg tctccatgcc gcagccgatc gcccccgagg agacgatgat gacatcgatg
      241 cccttctcct tgaccttcct gacctgccgg gcgatgtccc tgatcctgcc ggtggagatc
      301 ttcttctcat cgtcgagaag gacggaagtt cctatcttga tgacgagacg gttcatatgc
      361 gcgccgcttt tcttcatcct ctagaccgcc tggttgaggt tctgtctcaa cgcttcaacg
      421 ccctcggaga tcgccgaaac caactcgtcg acccccgcga gggtaagagc gctgacgacg
      481 accgccttct ctccctttct cttaaggtac gacctccacc gctgagccac gtccggagtc
      541 acgaggtcgg ccttgttgag tatcacgagc cttcgcttct gaagcatcgc ctcgttgtag
      601 ctccccaact cctcgaggag ggtcctgtag tctcccttca cgtcgtccga ggagaggtcg
      661 agaacgatga gaagcgcgtt cgttctctcg atgtgcttga ggaaggcaag acccaggcct
      721 ttccccttcg acgccccctc gatgatgccc gggatatcgg cgatgacgaa ggtcttctca
      781 ttgtcatcga ggacccccag ggccggggta agggtggtga agggatagtc gccgaccttc
      841 ggtctcgcct gggtcaggcg ggagatgagg gtcgattttc ccgcgttggg atggccgacg
      901 aggccgacat cggcgaggag cttgagatcg aggtgaagcc accgcctctg tccctcttcg
      961 ccggcatcga actccagagg ggcccggtgg gtggaggtga cgaacctggt gttccccctg
     1021 cctcccctgc cacccagggc tgccgcgtgc gtctcgccat ccctgaggac ctgaaacaga
     1081 agctctgagg tatcgcggtc gtaaacgacg gtgccgagag gcacgtgtat tgtgacgtcc
     1141 ctgccttccc tgccggtctt gttctttccc gctccggctt ttccgttctc agctttgtag
     1201 ttgcgcttgt atctgaaatc gtggaggctt gcgaggtctt tcttccctgc tatgataacg
     1261 tctcctccct tcccgccatc accgccgtcg ggcccgcctc gcggaatgaa cttttctcgt
     1321 cggaaactta cacagccact cccacctttt ccagactcaa cataaatgat cgcttcatct
     1381 atgaacttca tcaaacaggt acaacatgcg cccttcttct gtcacctgcc atgtcgaact
     1441 tcacgattcc gtctatcagg gcaaagaggg tgtcgtcctt gccgatgccg acattctgtc
     1501 cgggatggat cttcgttccg tgctgc
//
LOCUS       DAOPOA010000637         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_89441_length_1522_cov_2.884799, whole genome shotgun sequence.
ACCESSION   DAOPOA010000637 DAOPOA010000000
VERSION     DAOPOA010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89441_length_1522_cov_2.884799"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PKJ17_11260"
     CDS             complement(<1..293)
                     /locus_tag="PKJ17_11260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD76601.1"
                     /translation="MAHFGPHFGEEPPISGTRGSGNIFFSFCNLRCVFCQNYQISHEG
                     RGNTVTIDELVEMFFSLEAMGCHNVNLVSPTPYVPFIAAAIGEAKRRGIGIPFV"
     gene            complement(544..1005)
                     /locus_tag="PKJ17_11265"
     CDS             complement(544..1005)
                     /locus_tag="PKJ17_11265"
                     /inference="COORDINATES: protein motif:HMM:NF008675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOD76602.1"
                     /translation="MKKPGNDEFLHVINEMFSEQIPFNTLLGLSIESMSPGGVTVSFR
                     MRDELMGHRKRRMVHGGVISSVIDVTGGLVAFMGAQERMDETLGAKLERFGRVSTIDL
                     RVDFLRPGTGTAFTAIARTLRTGNKVAVTRTELTNDTGELIAVGTGSYTVS"
     gene            complement(1013..>1522)
                     /locus_tag="PKJ17_11270"
     CDS             complement(1013..>1522)
                     /locus_tag="PKJ17_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOD76603.1"
                     /translation="ALGVALERLELIESTRGTVAAWLLIAFGLAYTVWGIRRAVLNKP
                     HRHVHVHGTSDVHEHEHTHHGSHAHFHEDEGRAVTPWILFIIFVFGPCEPLIPLLIYP
                     AARKSFMGVALVSLAFGVVTIATMMAVVLLMRAGLSFKVLDRAERYTHAIAGLTILTC
                     GLMIQFLGL"
BASE COUNT          313 a          443 c          448 g          318 t
ORIGIN      
        1 acgaaaggta tgcctatacc tcttctcttc gcttctccga tggccgcggc gataaagggg
       61 acgtatggcg tcggactaac gagattgaca ttgtggcacc ccatcgcctc gagggagaag
      121 aacatctcca caagttcatc aatggtcacc gtgtttcccc gaccctcatg gcttatctgg
      181 tagttctgac agaagacgca ccgcaggttg cagaaggaaa agaatatgtt ccccgacccc
      241 ctcgtccccg agatgggcgg ctcctcccca aaatggggcc cgaaatgggc caccgcaata
      301 tccccgcctg ccccgcagaa ccccctttcc ccggcaaggc ggtcaacccc gcaccgacgg
      361 gggcagacag tgcagggtgc aagcatggaa aggtcgggtt tcatcggcaa tagtatagca
      421 gaaacggaag accaggtaat gggttatagg ttatgggtga taggaaaacc ggggaaaccc
      481 tgatttccac tgttctttgc tttttccccc atgacccatg acccggaacc cattacctgg
      541 tcttcaggag accgtatatg atcctgttcc caccgcgatg agctctcccg tgtcgttggt
      601 cagttccgtt ctcgtaacgg cgaccttgtt cccagttctc agggttcgtg cgatggcggt
      661 gaaggctgtg cccgtgccgg ggcggaggaa gtcgacccgc agatcgatgg tgctaacccg
      721 cccgaacctc tcaagcttgg cccccagcgt ttcgtccatt ctttcctgtg cgcccatgaa
      781 ggcgacgagt cctcccgtga cgtcgatgac ggaggatatc acgccgccat ggaccatacg
      841 gcgctttcgg tgtcccatga gctcgtcgcg catccgaaag gacaccgtca cgcctccggg
      901 gctcatcgat tctatgctga gcccgagcaa cgtgttgaaa gggatctgtt cgctgaacat
      961 ttcgttgatc acatggagga attcgtcgtt gccgggtttt ttcatcgttc cctcagagcc
     1021 cgaggaattg gatcatcagg ccgcaggtga ggatcgtcaa gcccgcgatg gcgtgggtgt
     1081 aacgttcggc cctgtcaagg accttgaagc ttaaacccgc cctcatgagg agtaccacgg
     1141 ccatcatcgt ggctatcgtt acgacaccga aggcgaggct cacgagggca acccccataa
     1201 agctcttacg ggcggcggga tagatgagaa ggggtatgag gggttcgcag gggccgaaga
     1261 cgaatattat gaagaggatc cagggtgtga cggcccttcc ttcgtcctca tggaaatggg
     1321 catggctgcc gtgatgggtg tgctcgtgct cgtggacgtc tgaggtgccg tggacatgca
     1381 cgtgcctgtg gggtttgttg aggaccgccc tgcgtatgcc ccacacggtg taggcaaggc
     1441 cgaaggcgat aaggagccac gccgcgaccg tcccccgcgt cgattctatg agctcaagcc
     1501 tctcaagcgc cacgccgagc gc
//
LOCUS       DAOPOA010000638         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_89518_length_1521_cov_3.219645, whole genome shotgun sequence.
ACCESSION   DAOPOA010000638 DAOPOA010000000
VERSION     DAOPOA010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89518_length_1521_cov_3.219645"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..>1521)
                     /locus_tag="PKJ17_11275"
     CDS             complement(159..>1521)
                     /locus_tag="PKJ17_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD76604.1"
                     /translation="KRVYPDDRLRQEAITTWQDEISGLPRGERKLKVFAVTCADGSQK
                     MVNFIPLKLASGDYLVTCEDITELKKLERQLRQSQKMEAIGSLAGGIAHDFNNILTTL
                     MGYAGLLQMEMDKHDPLGLHVEQIISASQKAANLTRSLLAFSRQQPIVLNPVSINDTI
                     RSTEKLLKRLLTEDVDFRVSLQPEDMVIMADATQIDQILFNLVANARDAMPRGGRLLI
                     ATSKTDMDERFVHTHGYGKPGAYVMVIVSDTGTGMDEATKEKIFDPFFSTKEVGKGTG
                     LGLSTVYGIVKQHGGFVNVYSEPGTGTTFRIYLPLVGSEAPRETEPELVTQRGTETIL
                     VAEDNADLRRLVTKVLTRHGYRAIGAVDGEDAINKHRKHRGIDLIIVDSVMPKKNGRE
                     VYEEIRKVDPGIAFLFTSGHTRDTILNRGIADSEFDFLPKPIAPFELLQTVRKVLDRK
                     KTA"
BASE COUNT          283 a          461 c          411 g          366 t
ORIGIN      
        1 tgatccggga tccagaggtc tctctaaccc atcgtttttc ctggattccg gccttcgccg
       61 gaatgacgac agaacgtgaa ctcagttcaa aaacccactt gatcaacagc ctgtcaagcg
      121 attcaatccc tttcttcccc tcaaacggtt caagcggctc aagcggtttt cttcctgtcc
      181 agcaccttcc tcaccgtctg cagaagttca aagggggcta tcggtttggg gaggaagtcg
      241 aattcggagt cggcgatgcc gcggttcaaa atagtgtccc ttgtatgacc gctggtgaag
      301 aggaacgcga tcccgggatc gaccttcctg atctcttcgt agacctctct cccgttcttt
      361 ttcggcatga cggaatcaac gatgatcagg tctattcccc tgtgcttccg atgcttgttt
      421 atcgcgtcct cgccatcgac ggcccctatg gccctgtatc catgcctggt gaggaccttc
      481 gtcacaaggc gcctcagatc ggcgttgtcc tcggccacaa ggatggtctc cgtacccctt
      541 tgcgtcacca gctcgggttc cgtctcccgc ggcgcctcgg agccgacaag gggaagatat
      601 atgcgaaagg tcgtgcccgt ccccggctcg ctgtagacgt tgacaaaccc gccgtgctgc
      661 ttcacaatgc cgtacacggt ggacagaccg agcccggtgc ccttgcccac ttccttcgtg
      721 ctgaagaaag gatcgaagat cttctccttc gtcgcctcat ccattcccgt tccggtgtcg
      781 gagacgatca ccatcacgta tgcgccgggc ttaccgtaac cgtgagtatg gacgaacctt
      841 tcgtccatgt cggtcttcga tgtagcgata aggagccttc ctcccctcgg catggcgtca
      901 cgcgcgttgg cgacaagatt gaaaagtatc tgatcgatct gggtggcatc ggccatgatg
      961 accatgtcct caggctgaag ggatacgcgg aagtccacgt cctccgtcag cagcctcttg
     1021 agaagcttct ccgtcgacct gatggtgtcg ttgatgctca cgggattgag gacgatgggc
     1081 tgttgcctcg agaacgcgag aagactccgg gtcaggttcg ccgccttctg ggaagccgag
     1141 atgatctgct cgacatggag ccccagggga tcgtgcttgt ccatctccat ctgcaaaaga
     1201 ccggcgtacc ccatcagcgt ggtgaggatg ttgttgaaat cgtgggcgat ccctcccgcg
     1261 agactgccta tggcctccat cttctgtgat tgccgcagct gccgttcaag cttcttgagt
     1321 tccgtgatgt cctcgcatgt caccaggtaa tcaccggaag caagtttcag ggggataaag
     1381 ttgaccatct tttgcgagcc gtcggcgcag gtgacggcga agaccttgag cttcctctct
     1441 ccccgcggca ggccgctgat ctcatcctgc caggtcgtga tcgcctcctg tctcagccta
     1501 tcgtccgggt agaccctctt g
//
LOCUS       DAOPOA010000639         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_89670_length_1519_cov_3.862705, whole genome shotgun sequence.
ACCESSION   DAOPOA010000639 DAOPOA010000000
VERSION     DAOPOA010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89670_length_1519_cov_3.862705"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1519
                     /locus_tag="PKJ17_11280"
     CDS             <1..>1519
                     /locus_tag="PKJ17_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOD76605.1"
                     /translation="LTGSTLSAPTMQMLNRTGLAERFKTHPAEVIAAIHKGVPTADET
                     DRIFALAELSFLYASQSGDRSHYLASAVYSWALLFPEGGARAPDAFDPRFRTAVDIYN
                     LGIAEGLTSADGVVIDLKEGTHKLPFGEVAISVRADSFRWSSYRLVRFADASKLSVRG
                     LRNEYRWPGIGAALVASTEYLPGAADPVSAKVPFDVKVAVTLLVRFHNVDEGLRTGRM
                     RGRLELYTTDSATAVTVGGRTVPLEFDLSSALAYTLENSGVYRMETKGFFSGNVTLFK
                     DASRFKENIFFMSPYMPGRIPVIFIHGTASSPARWAEMLNELQNDPRLWGRYQFWAFT
                     YNTGNPILYSGGLLTEGLKELVSGLDPEGRDPALRRMVIVGHSQGGLLTRLTVVDSGT
                     RFWDDAAKVPFDKLDVSPGTRGMISRSFFYKPLPFVSRVIFVSTPHRGSYVAGGWIGR
                     MAGRFVSLPFRMLDAVKEVTSLNPRAVDPSTFKGVPKSTANMDPNSQFVRTFSSIP"
BASE COUNT          276 a          473 c          451 g          319 t
ORIGIN      
        1 ctgaccgggt ccactctcag tgccccgacc atgcagatgc tcaaccggac cggtcttgcg
       61 gagagattca agacccatcc ggccgaggtg atcgcggcca tacacaaggg tgttccgacc
      121 gcggatgaga cggaccgcat cttcgccctg gcggagcttt cctttctgta cgcctcccag
      181 agcggagaca gatcgcacta tcttgcctcg gctgtatatt cctgggcgct tctctttccc
      241 gagggcggcg cccgcgcccc cgatgctttc gaccctcgct ttcgaacggc ggtggatatc
      301 tacaacctcg ggatagccga gggtctcact tccgccgatg gggttgtcat cgaccttaag
      361 gaaggtacgc acaaactgcc gttcggggag gtcgcgatct cggtccgtgc cgattcgttc
      421 cggtggagtt catatcgcct cgtcaggttt gccgacgcgt caaaactctc cgtccgggga
      481 ttgcgcaacg aataccgctg gccggggatt ggcgcggctc tcgtcgcctc caccgaatat
      541 cttcccggtg ccgcagaccc cgtctccgcc aaggtccctt ttgatgtcaa ggtggccgtc
      601 accttgctcg ttcggttcca taacgtggac gagggcctga ggacagggag gatgcgtgga
      661 agattggagc tgtacacgac tgacagcgcc accgctgtca cagttggagg gcgcacggtg
      721 ccccttgagt tcgatctttc ctcggcgctt gcctacaccc ttgagaactc gggggtgtac
      781 cgcatggaga cgaaggggtt cttttccggc aacgtgaccc tcttcaagga cgcgtcgcgg
      841 ttcaaggaga acatcttctt catgagtccc tacatgccgg ggcgcatccc cgtcatcttc
      901 atccatggga ccgcgtcgag cccagcccgc tgggcggaga tgctcaatga gcttcagaac
      961 gacccacggc tctggggccg ttaccagttc tgggccttta cctacaatac gggcaatccc
     1021 attctctatt cgggcggcct cctgacggag ggcctcaaag agcttgtcag cggacttgat
     1081 cccgagggca gggacccggc attgaggcgt atggtcatcg tgggtcacag ccagggagga
     1141 ctcctcacga gactgaccgt tgtcgacagc gggacccgtt tctgggacga cgccgcaaaa
     1201 gtccccttcg acaagctcga tgtgtcgccc ggtacgcggg ggatgataag ccgaagcttt
     1261 ttctacaagc ccctgccctt tgtcagcagg gtgatattcg tttcgacgcc gcaccggggc
     1321 agctacgtcg ccgggggatg gatagggaga atggccggca ggttcgtgtc gctccccttt
     1381 cggatgctcg atgccgtgaa ggaggtcacc tccctgaatc cccgggccgt cgacccgtcc
     1441 actttcaagg gggtcccgaa aagtaccgcg aacatggacc caaacagtca atttgtcagg
     1501 acattctcat cgatcccca
//
LOCUS       DAOPOA010000640         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_89672_length_1519_cov_3.776639, whole genome shotgun sequence.
ACCESSION   DAOPOA010000640 DAOPOA010000000
VERSION     DAOPOA010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89672_length_1519_cov_3.776639"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PKJ17_11285"
     CDS             <1..441
                     /locus_tag="PKJ17_11285"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOD76606.1"
                     /translation="LWWYIPLTFIFFPPFWLVMRRTPYLKALFSGTGRRERAVRDRAV
                     RAFYEKGLYRTKGNTGILFFISLLERKVWVLADKGIHEKITQAKLNAFARTVSAGIRQ
                     GTAADALVEAIGEAGRLLEEHFPTVEGDTNELTDEVITSDRETV"
     gene            601..1278
                     /locus_tag="PKJ17_11290"
     CDS             601..1278
                     /locus_tag="PKJ17_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOD76607.1"
                     /translation="MGEMDIYLRSTEIIDSDNGRIRETALKLAGGLTGEGPRARALFC
                     FVRDEIHYNVYMLSTFREDFRASTTLERGKGYCVQKAVLLAALARAAGIPSRLAFARI
                     VNHKMPAELKAQTGIEEFPSHGYTQLFVKGRWVSVTPAFDRGLCHRIGVPACDFDGEH
                     DAVLAPVDASGNPYVEYIEKYGPHADLPFEWLHSKIFPIWGNKRPWAEADASRGHIMP
                     LSGYRFP"
BASE COUNT          318 a          421 c          462 g          318 t
ORIGIN      
        1 ctctggtggt acatccctct cactttcatt ttcttccctc ctttctggct cgtgatgaga
       61 agaacgccat acctcaaggc acttttcagc ggcaccgggc gccgcgagcg ggccgtgcgg
      121 gacagggccg tcagggcctt ctatgagaag ggtctgtacc ggacaaaggg caacacgggc
      181 atcctctttt tcatctccct cctggaaagg aaggtttggg tccttgccga caagggcatc
      241 cacgagaaga tcacccaggc gaagctcaac gccttcgcga ggacggtctc cgcgggaata
      301 cggcagggga cggcggccga cgccctcgta gaggctatag gcgaggcggg caggctcctc
      361 gaggagcact tccccaccgt ggaaggcgac accaacgaac tgaccgatga agtcatcacg
      421 agcgacaggg aaacggtgta aagacgcccc caggtgcctt attcgtccta taggtcccag
      481 tcctataggt ccgatatgtc ttattggtcc tatgtgtttt ataataggac atatcggacg
      541 aataagacca ataggacgga tcgagggtgg ggggtcggtg gggtttttta gggagctgtg
      601 atgggtgaga tggatattta tctccggtcc acggagatca tagacagtga taacggtcgc
      661 atccgggaga cggcgctcaa gctcgccggg ggattgacgg gggaaggccc gagggctcgg
      721 gcgctcttct gcttcgtccg tgatgagatc cactataatg tttacatgct ctcgaccttc
      781 agggaagatt tcagggcgag cacgactctt gagagaggta agggctattg tgtccagaag
      841 gccgttcttc ttgcagctct cgcccgtgcc gcgggtattc catcccggct tgccttcgcg
      901 aggatcgtga accataagat gcccgcggag ctcaaggctc agacgggcat agaggagttt
      961 cccagtcacg ggtacaccca gcttttcgta aaggggaggt gggtgagcgt caccccggcc
     1021 ttcgacaggg gcctgtgcca caggatcggt gtgcctgcct gcgatttcga cggggagcac
     1081 gatgccgtcc ttgccccggt ggatgcttcc ggcaacccct acgtggagta catcgaaaaa
     1141 tatggacccc acgccgatct gccctttgaa tggcttcaca gtaagatctt ccccatctgg
     1201 gggaacaagc gtccctgggc cgaagccgat gcctcccggg gccacatcat gccattgtcg
     1261 ggataccggt tcccgtaagg gggtctcatc aagccgaaaa gtggttattg catcgggtgc
     1321 accaacaagc atggctgcaa gtccaggaca cctccctgca tcgacgagat gacatcccgc
     1381 aacgtcagtt ccgactcggg gaagcggtac ctcattgagc tcggcagaat agatctctgc
     1441 ggaggctgtc ccttcctgcg ctcctgctgg acaatggagg agtacggaaa aaagctggcc
     1501 tcaggttgat cttcccgta
//
LOCUS       DAOPOA010000641         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_89783_length_1518_cov_2.965824, whole genome shotgun sequence.
ACCESSION   DAOPOA010000641 DAOPOA010000000
VERSION     DAOPOA010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89783_length_1518_cov_2.965824"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PKJ17_11295"
     CDS             complement(<1..684)
                     /locus_tag="PKJ17_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76608.1"
                     /translation="MRTTMAGHKGLLRSPSVRIIGVAVVGVPLVLAILFLLYSHFQTE
                     KDLKENLLRVRHHTVERRAAALSYFFADRMDDLFYLKHSREVAAYFENRALGMSMEYG
                     LKQSLVPMRELFISLMKRKQIGDDRVYARLAYIADDGRSLVDTASRETGRGMADILKK
                     ARSSGGAGKISMSRDGEHFMVISAHEYKGEHRGHVVGWIRPSIIHRHILAADESAQSS
                     YLICDERGDP"
     gene            complement(684..>1518)
                     /locus_tag="PKJ17_11300"
     CDS             complement(684..>1518)
                     /locus_tag="PKJ17_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOD76609.1"
                     /translation="FPIADSSAVTLEEIRRNGVLRHLGIPYANFVTGSGDGMDVELMS
                     LFAERLGVRYEFVRTDWDRVFADITGKTFTRGDGNVIITGDAPVKGDVAASGITVLPW
                     REELVDFSLPVFPTQVWLMARADSSVKPVRPTGDLNRDVARTKELVRGRSIMGKTGTC
                     LDPSLYGLEKTADRVRPYGGSLNEIAPALMEGVADLVLLDVSDALVALQKWPGRIKVI
                     GPVSEMQEMAVAFRKDSPELKDEFNRFLADCKRNGTYTRIVKKYYPFVLEYHPAFFRR
                     K"
BASE COUNT          302 a          478 c          416 g          322 t
ORIGIN      
        1 ggggtctccc cgctcgtcac agatgaggta tgacgattgc gcggattcgt ccgcagcgag
       61 tatgtgcctg tggattatcg acggcctgat ccaccccacg acgtgtcccc ggtgctcgcc
      121 tttgtattcg tgtgccgata tgaccatgaa gtgctcacca tcccgggaca tcgatatctt
      181 tccggcgccg ccggaagaac gcgccttctt caatatgtcg gccatgcccc tgcccgtttc
      241 ccgcgatgcc gtatccacga gggatctccc gtcatcggca atgtaagcaa gtcgagcata
      301 gaccctgtcg tccccgatct gcttcctctt catgagggag atgaacaact cccgcatggg
      361 cacaaggctc tgcttcaagc cgtattccat gctcatcccc agtgccctgt tctcaaagta
      421 cgcggcaact tcccgggaat gcttcaggta gaaaaggtcg tccatcctgt cggcaaagaa
      481 atagctcagc gcagccgccc gtcgctcgac ggtgtgatgg cggaccctga gaaggttctc
      541 tttcaggtcc ttctcggtct ggaaatggct gtaaaggagg aagaggatcg cgagaacgag
      601 cggcacaccc acgacggcca ctccgatgat ccgcacggac gggcttctga gtagtccttt
      661 gtggcctgcc atcgtggtcc tcatcacttc cttctgaaga acgcggggtg atactccaaa
      721 acaaagggat agtatttctt cacgatcctg gtgtacgtgc cgttcctctt gcagtcggcc
      781 agaaacctgt tgaactcatc cttcagttcc ggagagtcct tcctgaatgc cacggccatc
      841 tcctgcatct cgctcacggg accgatgacc tttatacggc cgggccactt ctgcagcgcc
      901 acaagcgcat cggaaacatc aaggagaacg aggtcggcaa caccctccat gagcgcgggg
      961 gcgatctcgt tcagtgaccc gccgtacggc ctcacccggt ctgccgtttt ctccagtcca
     1021 tacagggaag ggtcaagaca ggtccctgtc tttcccatta tcgagcgccc cctgacgagc
     1081 tctttcgtcc gggcaacgtc gcggttcagg tcacctgtcg gtctcaccgg cttcacggac
     1141 gaatcggcgc gggccatgag ccagacctgg gtcgggaaca cgggaaggga aaaatcgacg
     1201 agttcctctc tccagggaag gacggtgata ccgctcgccg ccacatcacc cttgaccggc
     1261 gcgtcaccgg tgatgatgac gttcccgtca ccccgggtga acgtctttcc cgtgatgtcg
     1321 gcgaagacgc ggtcccagtc cgttctcacg aactcatatc ttaccccgag acgctccgcg
     1381 aagagcgaca tgagctcgac gtccattccg tccccgctgc cggtgacgaa attggcatag
     1441 ggtatgccga ggtgcctcag gacaccattc ctccttatct cttccagggt gacggcggag
     1501 gaatcggcga tgggaaaa
//
LOCUS       DAOPOA010000642         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_90228_length_1513_cov_4.572016, whole genome shotgun sequence.
ACCESSION   DAOPOA010000642 DAOPOA010000000
VERSION     DAOPOA010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90228_length_1513_cov_4.572016"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PKJ17_11305"
     CDS             <1..572
                     /locus_tag="PKJ17_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD76610.1"
                     /translation="PLEDGAVTISRVNHAITFPARFMLVAAMNPCPCGHFGDPKRSCT
                     CTATQIYRYRSRISGPLLDRIDIHIEVPPVTIAELSRERPEEPSAAIRERVSKARAIQ
                     LDRFNGRNIHANAQMSARVIRKHCPLDTPTRQLLEAAVDKWVLSPRAYHRILKVSRTI
                     ADLDASTDIAEAHVAEAIRYRALDKRLTI"
     gene            772..>1513
                     /locus_tag="PKJ17_11310"
     CDS             772..>1513
                     /locus_tag="PKJ17_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76611.1"
                     /translation="MYISVIGPALSGKTTLFQALSGVESARGFSDGDNIISIDVPDER
                     VDELSRIYRPKKTTYARIELADTVAISEGNVKDSTINAKTLQKMRMSDAFILTLNDFE
                     EAAAAELEKEFHGIMSEFILADMILIEGRLERIKKQAGKKENTALLLEEKLLEQCLAH
                     VGEGKPLRTLELAVSDEKTLRGFRFLSQKPMMIAINCSEDGMGEMPGEEERDMEGCPV
                     IHVCASLECELAMMEEAERAAFMEEFSVK"
BASE COUNT          354 a          465 c          442 g          252 t
ORIGIN      
        1 agcccctcga agacggcgcc gtcacgatct cccgggtcaa ccacgcgatc acctttcctg
       61 ccagattcat gctcgtggcc gccatgaacc cctgcccctg cggccacttc ggcgacccga
      121 aacgctcctg cacgtgcacg gccacccaga tctacaggta ccgctcccgc atatcgggcc
      181 cgctcctgga cagaatagac atccacatcg aggtgcctcc cgtaaccata gccgagcttt
      241 cccgcgagcg accggaagaa ccctccgccg ccatccgcga aagggtgtcc aaagcccgcg
      301 ccatacagct cgaccgtttc aacggaagga acatccacgc caacgcgcaa atgtccgccc
      361 gggtgatacg caaacactgt cccctcgaca ccccgacccg ccaattgctc gaagccgccg
      421 tggacaaatg ggtcctctcc ccccgtgcct accaccgcat tctcaaggta tcgcgcacaa
      481 tagccgacct cgacgcaagc accgacatag ccgaggcgca tgtagcggaa gcgatcagat
      541 accgtgcgct cgacaagaga ctgaccattt gaaaggcggc ttgaaccact tgaatggctt
      601 gaaccacttg aatggcttga accgcttgag cggcttgaac gtcaggggaa aaggagctat
      661 aatggtggcc caggctggca ttacgggcgt tcgaggtcag cggtacaatc gcatgaaagc
      721 cgatggtcgg gccgcgatgg cttcctgaga tatgagacag gggagaagag gatgtatatt
      781 tccgtcattg ggccggctct ctccggcaag acaacgcttt tccaggcgct gagcggcgtg
      841 gagagcgcgc gggggttttc cgacggggac aacatcatct ccatcgatgt acctgatgag
      901 cgcgtcgacg agctgtcgag gatctacaga ccgaagaaga ccacctacgc gcggatagaa
      961 cttgccgaca ccgtcgcgat atcggagggc aacgtcaagg actccaccat caacgcgaag
     1021 acgctgcaga agatgcgcat gagcgatgcc ttcatcctca cactcaacga ttttgaggag
     1081 gccgctgcag cggagctcga gaaggaattc cacggcataa tgagcgagtt catactggcc
     1141 gacatgatcc tcatcgaagg acgcctcgag cgcatcaaga aacaggccgg gaagaaggag
     1201 aacacggccc ttctcctgga ggaaaaactc ctcgagcaat gcctcgccca cgtgggggag
     1261 ggcaaaccgt tgaggacgct cgagcttgca gtgagcgatg agaagacgct caggggtttc
     1321 aggttcctct cccagaaacc catgatgatc gccatcaact gctccgagga cgggatgggc
     1381 gagatgcccg gggaggagga gcgggacatg gagggctgcc ccgttatcca cgtctgcgcc
     1441 agtctggagt gcgagcttgc catgatggag gaggctgaac gcgccgcctt catggaggag
     1501 ttctccgtca agg
//
LOCUS       DAOPOA010000643         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_90488_length_1510_cov_2.884536, whole genome shotgun sequence.
ACCESSION   DAOPOA010000643 DAOPOA010000000
VERSION     DAOPOA010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90488_length_1510_cov_2.884536"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKJ17_11315"
     CDS             <1..490
                     /locus_tag="PKJ17_11315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOD76612.1"
                     /translation="GADFVFEMLEHGKVKVEKKGLDRRITLMQGDALELPFRDNSFDV
                     VGIAFGIRNIPDRERALKEMLRVTVPGGAVMVLEMTFIQNPLFKLVYHVYLNHILPRL
                     AKRFSPNPAAYHYLADSIMNFPQPDAFAAMMEKTGMVNVTKHPLTFGVTYLHVGVKPE
                     GL"
     gene            490..1383
                     /gene="menA"
                     /locus_tag="PKJ17_11320"
     CDS             490..1383
                     /gene="menA"
                     /locus_tag="PKJ17_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOD76613.1"
                     /translation="MAGGTLKAWVQASRMPFFVATFIPLFIGWSMAVRATGAVRPGRF
                     LIVLLGSLIVHLITNLANDYFDHLVGTDAGESIGGSRVIQEGKISPQVMLRVIIVLYV
                     IAFFIAYIIVFHFGLHLLAIPILFSAFSSYFYVAPPIRYGYHGLGELFVGINMGPVMV
                     AGTYWVVAGAPALEPLLVSIPVGIMVASILYYQSLPDMKTDEASGKMTLAVRLGRKGA
                     FRGLIAFFILIYASILWLILMGILSPWALASIVSALLAIKLIRIVLRTSDWVLLDLHG
                     KYIRMVYFICGLSIIAGILGT"
BASE COUNT          272 a          498 c          407 g          333 t
ORIGIN      
        1 cggcgccgat ttcgtcttcg agatgctgga acacggaaag gtgaaggtcg aaaagaaggg
       61 gctcgaccgt cgcatcaccc tcatgcaggg cgacgccctc gaacttcctt ttcgagacaa
      121 ctccttcgac gttgtcggca tcgccttcgg tatcaggaac atccctgacc gcgaaagggc
      181 cctgaaggag atgcttcgcg tcaccgtccc cgggggcgcc gtgatggtcc tcgaaatgac
      241 cttcatacag aacccgctgt tcaaactggt ctaccacgtc tatctcaatc atatcctccc
      301 gcggctggcg aaacggttct ctcccaaccc cgctgcgtat cactacctcg ccgattcgat
      361 catgaacttc cctcaacccg acgcttttgc ggccatgatg gaaaagaccg gcatggtcaa
      421 cgtgacgaag catcccctca ccttcggcgt cacctacctc cacgtgggcg tcaaaccgga
      481 aggcctgtga tggcgggcgg caccctgaag gcgtgggtcc aggcgagcag gatgcctttc
      541 ttcgtggcca ccttcatacc ccttttcatc ggctggtcga tggccgtcag ggcaacgggc
      601 gccgtcaggc ccgggcgctt cctcattgtc cttctcggct cgctcatcgt ccacctcata
      661 acaaacctgg ccaacgacta cttcgaccac ctcgtgggga ccgatgccgg ggagtcgatc
      721 ggaggctccc gtgtcattca ggagggtaag atcagcccgc aggtgatgct gagggtgata
      781 atcgtccttt acgtcatcgc cttctttatt gcctacatca tcgtctttca cttcgggctc
      841 cacctcctcg ccatccccat cctcttctcc gccttcagca gttacttcta cgtcgccccc
      901 cctatacggt acggttatca cgggctgggc gaactcttcg tggggatcaa catgggtcct
      961 gtcatggtcg cggggacata ctgggtcgtg gcgggcgccc ccgcgctcga acctcttctc
     1021 gtttccatac ccgtcgggat catggtggcc tccatcctct attaccagag cctcccggac
     1081 atgaagaccg acgaggcctc cgggaagatg accctcgccg tcagactcgg cagaaaaggc
     1141 gccttccggg ggctgatcgc cttcttcatc ctcatctacg cgagcatcct ctggctcatc
     1201 ctcatgggga tcctgtcgcc ctgggcgctt gcgtcgatcg tcagcgcgct cctcgccatc
     1261 aaactcatcc gtatcgttct gaggacgagc gactgggtcc ttctcgacct ccacggcaag
     1321 tacatcagaa tggtctattt catctgtggc ctgtcgatca tagcgggaat cctggggacc
     1381 tagcgcacgg gtcggcgctg cgcgcctacg ggcaaacggg catacgggga agggcaagac
     1441 cgttccatct tatgcgttac ggaaatgtcc ggggttcccg tcagaggcct ttcctcgcgt
     1501 gcccgtaggc
//
LOCUS       DAOPOA010000644         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_90517_length_1510_cov_2.327835, whole genome shotgun sequence.
ACCESSION   DAOPOA010000644 DAOPOA010000000
VERSION     DAOPOA010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90517_length_1510_cov_2.327835"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1510)
                     /locus_tag="PKJ17_11325"
     CDS             complement(<1..>1510)
                     /locus_tag="PKJ17_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76614.1"
                     /translation="AMPFNAVLETGDLNFSSLFAMAKTLMTEEEAKKWSVEGLGSLKA
                     RMEGSLGGKTRSVSGTLTMDIRKGGFSSPDGSKAAQGITGSVILNFSLPRGEDREGSV
                     KISSQVSSGEYLWGRYYKDLRKEPSRLSSRADVTVSGKGGSRFRGTFDLFNTGRYAYD
                     GFVNAGEWGVHVTAQDVPVRRAFDLFAADYLAQTAAVLKGMEIDGKLDADVSVTSAGK
                     GLGTKGSVRLSAASLKLPAISLGVAGIDVDIPFDLASAGSGDGASLPPGPLGIVTITG
                     FTKGAYSLPELKIPVTAYGNDVSAPGIISVPFYDGTVRVRTLTAKDILGASPKLLFGV
                     SMRDIDAQKLLSDLTGLAFPGKVKARFPEIAYQNGELLTEGKMTVDIFGGTIEVADMY
                     VKDLFLASRKIGGDILFRNIDLGKVTETIKVGRITGIIEGSVKDLVVEYGQPSRFVFD
                     LDTVKKWGQSQKVSVDAIENISILGTGSGGIGAVLKSGLNKFFKEYPYSRIGI"
BASE COUNT          285 a          483 c          432 g          310 t
ORIGIN      
        1 gtatgccgat gcggctgtac gggtattcct tgaaaaactt gttgaggccg ctcttgagga
       61 ccgctccgat gccgccggaa ccggtgccga ggatggatat gttctcgatg gcatcgacgg
      121 acaccttctg ggattgtccc catttcttga ccgtgtcaag gtcaaacaca aacctcgatg
      181 gttgaccgta ttcgacgacc agatccttca cggagccctc gatgatgccc gttatcctgc
      241 cgaccttgat cgtctccgtc accttcccga ggtcgatgtt cctgaagaga atgtctccgc
      301 ctatcttccg cgaggccagg aagaggtcct tcacgtacat gtccgccacc tctatcgtgc
      361 ccccgaagat gtcgacggtc atcttcccct ccgtcaggag ctctccgttc tggtaggcga
      421 tctcgggaaa gcgcgccttg accttcccgg gaaaggcgag gcccgtgagg tcgctcaaga
      481 gcttctgggc gtctatgtcc cgcatcgaga ccccgaacaa cagcttcgga gatgcgccga
      541 ggatgtcctt tgcggtgagg gtcctcacgc ggaccgtacc gtcgtagaag ggaacggata
      601 tgatccccgg ggccgatacg tcgtttccat aggcggtgac gggtatcttc agttctggca
      661 ggctataggc gcccttcgtg aacccggtga tggtgacgat gccgagaggt cccggcggga
      721 gggaagcacc atctccgctc ccggccgacg cgagatcgaa ggggatatcc acgtcgatgc
      781 ctgccacacc aagggagatc gcgggcagtt tcaacgacgc ggcggaaagc ctcacgctcc
      841 ctttcgtgcc gagtcctttc cccgccgatg tgaccgacac atcggcgtcg agctttccgt
      901 caatctccat gcctttcagc accgccgccg tctgggcgag ataatcggcc gcgaagaggt
      961 cgaaggccct tctcaccgga acatcctgcg ccgtcacatg cacgccccac tcaccggcgt
     1021 tcacgaaacc gtcgtaagca taccgccccg tgttgaagag gtcgaaggtc cccctgaacc
     1081 gggaaccccc tttgccgctt accgtcacat cggcccggga ggaaagcctt gagggttcct
     1141 tcctgagatc cttgtaatac cgtccccaga ggtactcccc ggaggatacc tgtgacgata
     1201 tcttcacgga gccctccctg tcctcgccgc ggggaaggct gaaattgagg atgacggaac
     1261 ccgtgatgcc ctgggccgcc ttcgagccgt cgggagagga aaagccccct ttcctgatgt
     1321 ccatggtgag cgtccccgaa acggaccggg tcttgccgcc gaggctccct tccatccggg
     1381 ccttaaggga acccagtcct tcgacggacc acttcttcgc ctcttcctcg gtcatgagcg
     1441 tcttcgccat cgcgaagaga ctggagaaat tgaggtcccc tgtctcaagg accgcgttga
     1501 aaggcatggc
//
LOCUS       DAOPOA010000645         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_90651_length_1508_cov_2.977288, whole genome shotgun sequence.
ACCESSION   DAOPOA010000645 DAOPOA010000000
VERSION     DAOPOA010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90651_length_1508_cov_2.977288"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..417
                     /gene="nifU"
                     /locus_tag="PKJ17_11330"
     CDS             22..417
                     /gene="nifU"
                     /locus_tag="PKJ17_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOD76615.1"
                     /translation="MAQGPYSDTVMDHFEHPRNMGEMDGADGMAQVGNPACGDVTRIF
                     LKIENDRIVDVKFKTFGCAAAIASSSMTTELIKGKTIDEALKLTNEIVARELGGLPEA
                     KMHCSVMAEEALRAAIEDYRKKQGEQGDP"
     gene            414..797
                     /locus_tag="PKJ17_11335"
     CDS             414..797
                     /locus_tag="PKJ17_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOD76616.1"
                     /translation="MTEFHVRTSKRIEALNITSQVVNAARGKAGKILHVYTPHTTCAI
                     TINEYADPDVVRDIIEALDRMVPAGYPYRHGEGNSDAHIKAVLVGASVSIPLSGDVPA
                     LGTWQGIFLMEFDGPRERKVQITVT"
     gene            841..1305
                     /locus_tag="PKJ17_11340"
     CDS             841..1305
                     /locus_tag="PKJ17_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76617.1"
                     /translation="MTRLKTICAITLCLVFLGASATIAFAKNEKVATPKEYGAYIKTS
                     KGLIRLLPNIVFDEDGVIFVESNNPAHFFLKDFQYFVLYGKYEMSVLTVNPMGFYQSS
                     ALGKPRFAFGKDIEIDVKPQGKDMYIIKPKGLLGRGYYSIWINDTAWDFMLD"
BASE COUNT          388 a          408 c          437 g          275 t
ORIGIN      
        1 ggtgaacaac aggaggtaat gatggcgcag ggcccctata gcgatacagt gatggaccat
       61 ttcgagcatc ccaggaacat gggcgagatg gacggagccg atggcatggc acaggtgggc
      121 aaccccgcgt gcggtgacgt gacgaggatc ttcctcaaga tagagaacga ccgcattgtc
      181 gatgtcaagt tcaagacctt cggctgcgcg gccgcgatcg cgtcaagcag catgaccacg
      241 gaactcatca agggaaagac catcgatgag gctcttaagc tcacgaacga gatcgtcgcg
      301 cgggagctcg gcgggctccc cgaggccaag atgcactgtt cggtcatggc ggaagaggcc
      361 ctcagggccg ccatcgaaga ctacaggaag aaacaggggg aacagggcga tccatgacgg
      421 agttccatgt cagaacgtcg aaaagaatag aggccctgaa cataaccagc caggtcgtga
      481 acgcggcaag gggaaaggcc gggaagatcc tccatgtcta caccccccac accacgtgcg
      541 cgattacgat caatgaatac gctgaccccg atgtcgtgcg tgacatcatc gaagccctgg
      601 acaggatggt tcctgcgggg tatccgtacc ggcacgggga gggaaattcc gatgcccaca
      661 tcaaggccgt gctcgtcggg gcgtcggtga gcatcccgct ttccggggac gtgcccgcgc
      721 tgggaacctg gcagggaatc ttcctcatgg aattcgacgg accgcgggag agaaaggtcc
      781 agatcacggt gacatgagat agcaacacgc gtgccgaaag aacgataaat ggagaaacca
      841 atgacaagac tgaagacaat atgcgccata acactgtgtc tcgttttctt aggggcctct
      901 gcaacaatag ccttcgcgaa gaatgaaaag gtcgcgacac cgaaggagta cggggcatac
      961 atcaagacga gcaagggact catcaggctc ctgcccaaca tcgtcttcga tgaggacggg
     1021 gtcatcttcg tggagtccaa caatcccgcc cattttttcc tcaaggattt ccagtacttc
     1081 gtcctctacg gcaaatacga aatgagcgtg ctcaccgtga accccatggg cttctatcag
     1141 tcgtcggcac tcggcaaacc acgcttcgcc ttcggcaagg acatcgagat cgacgtcaaa
     1201 ccccagggca aagacatgta catcatcaaa cccaaggggc tgctcggcag gggatactac
     1261 agcatctgga tcaacgacac cgcatgggat ttcatgctcg actgacaccg gcttgaaccg
     1321 cttgaatggc ttgagccgtt tgagcgttga aagacaaaaa aaatgacccc cgttgtgctc
     1381 ggccacacag gggtcgttct ttttctaacg ttcaagcggc tcaagccatt caagcggttt
     1441 aagccggtgt tttttggagg cggcatccgg atttgaaccg gagaataacg gttttgcaga
     1501 ccgtcgcc
//
LOCUS       DAOPOA010000646         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_90733_length_1507_cov_3.739669, whole genome shotgun sequence.
ACCESSION   DAOPOA010000646 DAOPOA010000000
VERSION     DAOPOA010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90733_length_1507_cov_3.739669"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1133)
                     /locus_tag="PKJ17_11345"
     CDS             complement(144..1133)
                     /locus_tag="PKJ17_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76618.1"
                     /translation="MITGIGSFPFTSVDETIDLIFSTCKGIPFWPQLPKRSLLENMYT
                     TFLEGVPCIVSDEEKNTIFMNTEGTDGIEEFYENVANDNVGAFAISERYAPGFHRFLE
                     RLREIEDGLQFIKCQVTGPFSMGLGLKDENDKPVIYNFAFYDIIKKALHMKAKWMIKT
                     IQSAYPGVDIIIFFDEPYMVSFGSAYISIPREEVIASLNEVVEDLPARRGIHVCGNTD
                     WSVPFQSDIDIVNYDAFSYLDTVFYFNEDLKEFLDRGGWIAPGVVPSSPEVMQETPDT
                     VRDRAASFVEKIASVTAPDMDSILFTTSCGLGSLTEEEARRAMELLRQIAYGE"
BASE COUNT          325 a          415 c          430 g          337 t
ORIGIN      
        1 tcaaaggaat tgctggacca gttgttcaca aaaaagggat aaccgggaag caagcaagag
       61 aggccggagg aaaccctccg gcctctctgg ttatcagctc atcgccctat cacctatgac
      121 ccatcaccca tgacccgcct cttttactcc ccgtacgcga tctgcctcag cagctccatg
      181 gcccggcggg cctcttcctc ggtgaggctt ccgaggccgc agctggtggt gaagaggatg
      241 ctgtccatgt cgggggcggt gacggacgcg atcttttcca cgaaggacgc ggcacggtcc
      301 cgcaccgtgt cgggcgtctc ctgcatgacc tccggcgacg aagggacgac acccggcgcg
      361 atccagcctc ccctgtcgag aaactccttg aggtcctcgt taaagtagaa gaccgtatca
      421 aggtaggaga aggcgtcata gttcacgatg tcgatgtccg actggaaagg gacggaccag
      481 tccgtgttgc cgcacacgtg aatgccgcgg cgggcgggca ggtcttcgac gacctcgttc
      541 agggaggcta tgacctcctc ccggggaatg gatatgtagg cggacccgaa ggagaccatg
      601 tacggttcgt caaagaagat gatgatatcc acgccggggt atgccgactg gatggtcttt
      661 atcatccact tcgccttcat gtggagggcc ttcttgatga tgtcgtagaa cgcgaaattg
      721 tagatgaccg gcttgtcgtt ctcatccttg aggccaaggc ccatgctgaa cggtcccgtc
      781 acctggcact tgatgaactg aaggccgtcc tctatctcgc gaagcctctc gagaaagcgg
      841 tggaaaccgg gtgcgtatct ttccgatatg gcgaaggccc cgacgttgtc gttcgcaacg
      901 ttctcgtaga attcctcgat cccgtcggtc ccctcggtgt tcatgaagat ggtgttcttc
      961 tcttcgtcgc ttacgatgca gggcacaccc tccaggaagg tggtgtacat gttctccagc
     1021 agggaacgct tcggcagttg cggccagaag ggtatcccct tgcaggtgga aaaaatgaga
     1081 tcgatcgttt catcgacact cgtaaaagga aaactgccga taccggttat cattatctcc
     1141 tcgtaagtgg actttgcact gctatctggg gaaatttcta tcacacgtgg cgggatgaag
     1201 taaagagaag agggggaaag gagagggaag aagcagatag gacctatagg aatcaataag
     1261 acctataggt cctatcaaaa cccctcccct gccgcctcat cccctcacct ctcttcttgt
     1321 caatccccgc catacctgtt atcttatccg cacaatatca caatacaggg gatgatcggg
     1381 tgacgatata caatggggtt tccgcggcgg gtgtgctctg ttttctcggg ttcctgtggc
     1441 ttctctcaag gcacaggcgg cgggtgaatc cccgggtggt catctggggt ctggcgctcc
     1501 agatcgt
//
LOCUS       DAOPOA010000647         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_90894_length_1505_cov_2.990345, whole genome shotgun sequence.
ACCESSION   DAOPOA010000647 DAOPOA010000000
VERSION     DAOPOA010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90894_length_1505_cov_2.990345"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1505)
                     /locus_tag="PKJ17_11350"
     CDS             complement(<1..>1505)
                     /locus_tag="PKJ17_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76619.1"
                     /translation="SNISLIRSEFHRVLAEGRWFTRGTKRIAAVKETDGRVPDDLRRV
                     VFAGLFGLTGGERKILRSFWDAGQTTVLLSGDPEDWPVLKDLVGHLKAKVEYGDAARN
                     ERPVVNLHAGHDTHAEVLEAYRILKESPRKKTAIVLPDADPLFPLLSFVADRTELKCN
                     ISLAYPMDRTPVFDLTRAVLAARVERRTDGQYPSARYLAVILHPFIKNLEPDSNLRSL
                     LVHIERSLSDETGKGPLAGRSLVTLAEIEEAAAALDPSGQAGHLAALLDVHRLFFKNF
                     ENLSTVGDVAAALEEALEAIIFKTPVRSYVLSGTIFESIFGALGSLKGSLFSSSPLSD
                     DPADNTRTACEITLHHLENTTLPFDTHPVEELEIIGMLESRNLSFDRVIILDVNEGVL
                     PGPREVSPVIPVGVFETLGIPSPEFTEAIYRYNFYRLTGSAGEVHLIYRSSEDRPRSR
                     YIEEILWEEEKSQGRLDVIPIRQTVVTVNLKRQTAPPAIETALPAAVSNTP"
BASE COUNT          270 a          432 c          495 g          308 t
ORIGIN      
        1 ggggcgtatt cgagacggct gcgggcagtg ccgtttcgat cgcggggggc gccgtttgtc
       61 ttttcaggtt gacggtgacc accgtctgcc ggatggggat gacatcgagc cgtccctgcg
      121 acttctcctc ctcccagagg atctcctcga tgtacctgct tcttggcctg tcctcggagc
      181 tgcggtatat tagatgcacc tccccggcgc ttccggtgag gcggtagaaa ttgtagcggt
      241 atatcgcctc cgtgaactcg ggtgatggga tgcccagcgt ctcgaagacg ccaacgggga
      301 taacgggact cacctccctc ggtcccggaa ggacaccctc gttcacatcg aggatgatga
      361 ccctgtcgaa ggacagattc cttgattcga gcatgccgat gatctccagt tcctccacgg
      421 ggtgggtatc gaagggcagg gtggtattct ccaggtgatg gagggtgatc tcgcaggcgg
      481 tacgcgtgtt gtcggcggga tcgtcggata ggggcgagga actgaagaga gagcccttaa
      541 gggagcccag ggctccgaag atgctctcga agatcgttcc cgatagcacg tatgaacgca
      601 ccggcgtctt gaagattatc gcttcaaggg cctcttccag ggcggcggca acgtccccga
      661 cggtggacag gttctcgaaa ttcttgaaga acagccggtg cacatcgaga agtgcggcca
      721 gatggcccgc ttgtcctgac ggatcaaggg ccgccgccgc ctcctctatc tcggcaaggg
      781 tcacaaggga ccggcccgcc agcgggccct ttccggtctc gtccgagagg gagcgctcta
      841 tgtgtacgag gaggctcctg aggttggagt cgggttcaag gttctttatg aaagggtgga
      901 ggatgacagc gagataccgt gccgagggat actgtccgtc cgtccttctc tcgacgcgcg
      961 cggcgaggac ggcgcgcgtg aggtcaaaga ccggtgtcct gtccatgggg tatgccagag
     1021 agatgttgca tttcagttcc gtcctgtccg cgacgaaact gaggagcggg aagagggggt
     1081 cggcgtcggg aagaacgatg gcggtcttct tccgtgggga ttccttgagg atgcggtatg
     1141 cctcgaggac ctccgcgtgc gtatcgtggc ccgcgtgaag attgaccacc ggccgttcat
     1201 tcctggcggc atccccgtat tccacctttg ccttcaggtg gccgacgagg tctttgagca
     1261 caggccagtc ctccgggtcg cctgagagga gcacggtggt ctgtccggcg tcccagaagg
     1321 acctgagaat cttcctctcc cctcccgtga ggccgaagag cccggcgaag acgacgcgtc
     1381 gaaggtcatc ggggacacgc ccgtccgttt ccttcaccgc ggcgatgcgc ttcgtgcccc
     1441 tcgtgaacca ccggccctcg gccagaaccc ggtgaaactc gctcctgatc agagaaatat
     1501 tggac
//
LOCUS       DAOPOA010000648         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_91132_length_1502_cov_3.132688, whole genome shotgun sequence.
ACCESSION   DAOPOA010000648 DAOPOA010000000
VERSION     DAOPOA010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91132_length_1502_cov_3.132688"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKJ17_11355"
     CDS             complement(<1..219)
                     /locus_tag="PKJ17_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76620.1"
                     /translation="MNGRLPAYKKSFFLSPERPDGLQLQIFHEDGIVYSDLFIDNRFE
                     GYADVLQGGMIFGILDVIAFVPRILRNAI"
     gene            complement(216..1304)
                     /gene="rlmN"
                     /locus_tag="PKJ17_11360"
     CDS             complement(216..1304)
                     /gene="rlmN"
                     /locus_tag="PKJ17_11360"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOD76621.1"
                     /translation="MEEFFGLTLSRLEAQIGALGKEKYRARQLFRWVYNQGNLDFREM
                     TNIPKSLRSVFSEMFSLDTMPIAEERSSRDGSTKLALVTRDAHVIEVVIMPEKSRVTL
                     CVSTQIGCRMGCRFCVTGKIGFKRNLSAAEIVGQVITAREYLRKAGKSPVTNIVFMGM
                     GEPMDNIDNVATALDILKDQSGLDLSYRKITLSSVGLVDGLKTLSAKSAVIAISLNAA
                     DDETRSMLMPINRLYSIGRIMDFARGFKGTKRTRITFEYILIKGVNDSLDDAKRLADL
                     LTGVKCKINLIPYNPSSYTEFERPERGEVDRFHQYLIERYFTVIIRDSRGQDVGGACG
                     QLGMGYLGGAPDPAAPDTKETTASEVTS"
BASE COUNT          295 a          435 c          458 g          314 t
ORIGIN      
        1 gatcgcgttc ctcaggatgc gcgggacgaa ggcgatgacg tcgaggatgc cgaatatcat
       61 gccgccctga aggacatcgg cgtagccctc gaagcggtta tctatgaaga ggtcggagta
      121 gacgatgccg tcctcgtgga atatctggag ctggagcccg tccggcctct cggggctcag
      181 gaagaaggat ttcttgtacg ccggaagtct gccgttcatg atgtcacctc ggaagctgtc
      241 gtctctttcg tgtcgggggc agcggggtcc ggggcacctc cgaggtagcc catgccgagc
      301 tgcccgcatg ccccgccgac gtcctgcccg cgggaatcgc ggatgatgac cgtgaagtac
      361 cgctcgatga gatactggtg gaacctgtcc acctcacccc tctccggtct ctcaaattcg
      421 gtgtacgacg aagggttgta gggtataagg ttgatcttgc acttcacgcc cgtgaggaga
      481 tccgcgagcc tcttcgcgtc gtcgagggaa tcgttgaccc ccttgatgag gatgtactcg
      541 aaggtgatcc tcgtccgttt cgtccccttg aagccccgcg cgaaatccat gatcctgccg
      601 atggagtaaa gcctgttgat gggcatcagc atggaccgcg tctcatcgtc ggcggcattg
      661 agggagatgg cgataacggc ggacttcgcg ctgagcgtct tgagcccgtc gacaagcccg
      721 acggaggaaa gagtgatctt gcggtaggaa aggtcgaggc cgctctggtc cttcaggatg
      781 tcgagggctg tggcgacgtt gtcgatgttg tccatgggtt cgcccatccc catgaagaca
      841 atattcgtca cggggctctt tccggccttc ctcaggtatt cccgagcggt gatgacctgt
      901 cccactatct ccgccgcgct gaggttcctc ttgaagccta tcttgcccgt cacgcagaac
      961 ctgcatccca tgcggcagcc gatctgcgtg gagacacaga gagtgacgcg actcttctcg
     1021 ggcatgatga cgacttcgat gacgtgcgcg tccctcgtca cgagggcgag cttcgtcgag
     1081 ccgtcccggg aggacctctc ttcggctatg ggcatggtat cgagagagaa catctccgag
     1141 aaaacactcc tcaagctctt ggggatgttc gtcatctccc ggaaatcgag gttaccctgg
     1201 ttgtagaccc agcggaaaag ctggcgggca cggtatttct ccttgcccag cgctcctatc
     1261 tgcgcctcga gacgggaaag ggtgagacca aaaaactctt ccattgcttg actgactctc
     1321 cggtgcgggt ccttgacgaa cgcgaaacac catacgatac cagaaaaagc tcattctttc
     1381 aagatattca gagggttaac gccaatggcg gcggggagga tggccgcggt gaccccagta
     1441 accccgggga ggaccgtgcc accgccggta gcccgaccat acgggatatc ggggacccca
     1501 cc
//
LOCUS       DAOPOA010000649         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_91148_length_1502_cov_2.614375, whole genome shotgun sequence.
ACCESSION   DAOPOA010000649 DAOPOA010000000
VERSION     DAOPOA010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91148_length_1502_cov_2.614375"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PKJ17_11365"
     CDS             <1..285
                     /locus_tag="PKJ17_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOD76622.1"
                     /translation="YTNILRERGAVRRDALLEAGPVRLRPILMTTFAMIMGMMPIALG
                     IGEGAETRSPMALATIGGLITSLLLTLVVVPVAYDLFDEMKERYFPRKDG"
     gene            288..578
                     /locus_tag="PKJ17_11370"
     CDS             288..578
                     /locus_tag="PKJ17_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76623.1"
                     /translation="MKMLIIVYNDVVDEELIATLKKAGVSGYTKWKEALGEGPGTEPK
                     LATNIWPGKNNVIAVVVKDEELPAVSGLLRDLKGSHPKGGIKAFILEVEGTI"
     gene            666..>1502
                     /locus_tag="PKJ17_11375"
     CDS             666..>1502
                     /locus_tag="PKJ17_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOD76624.1"
                     /translation="MSLFFVTFFTLYTALHAYVFIKAWYAFRFRPLTGCILALFLVFC
                     VFMPLFVRALEQGGQESIARVFAYAGYIWMSAIFIFFATSLVIDIGRLCVIIASSLFK
                     VGLPATMRSNLVLFLIPVAVSVFTVVYGYHEALSIRTERIRIETSKLPAGIEKVTIVQ
                     VSDVHLGLVNRERVLKRVVGAIRTARPDVVVSTGDLVDGQINNINGLSDMLRDIKPRY
                     GKYAIMGNHEFYAGVGVSEDFTRKAGFRMLRNEGITIDGVMNMAGVDDAGHRSTDRHS
                     PGD"
BASE COUNT          303 a          412 c          449 g          338 t
ORIGIN      
        1 tacacgaata tcctcaggga gcgcggcgcc gtccggcggg atgccctgct cgaggcaggg
       61 cctgtcaggc tccgtcccat actcatgacc accttcgcca tgataatggg catgatgccc
      121 atcgccctcg ggatcggtga gggcgccgaa acgcggtcgc ccatggctct cgccaccatt
      181 ggtggcctta tcacctcgct gctcctcacg ctcgtcgtcg ttcccgtcgc ctatgatctc
      241 ttcgatgaga tgaaggaaag gtactttccc cggaaggacg gttgaagatg aaaatgctca
      301 tcatcgttta caatgacgtc gtcgacgagg agcttatcgc gacgttgaag aaggcgggtg
      361 tgtccggata caccaagtgg aaggaagcgt tgggcgaagg gccggggacg gagcccaaac
      421 tggccacgaa catctggccc ggcaagaaca acgttatcgc tgttgtcgtg aaagacgagg
      481 aacttcccgc ggtttcgggg ctccttcggg atttgaaggg gtcccatccg aaagggggca
      541 taaaggcctt tatccttgag gtcgagggaa ccatttagcc ccgccccttt accgggatgg
      601 cccgtcgtgc ttatcttatg aaccatgacc tgtctggatg aaggtagaga ttgtaaggat
      661 atgaaatgag cctgttcttt gtaacgtttt tcacgctcta tacggccctt cacgcgtacg
      721 tattcataaa agcgtggtac gcctttcggt tccgcccgct cacgggttgt atcctcgccc
      781 tctttctcgt gttctgcgtc ttcatgcccc tctttgtgcg cgccctcgaa cagggagggc
      841 aggaatccat cgcccgcgtc ttcgcctatg ccggttacat ctggatgagc gccattttca
      901 tcttctttgc cacatcgctg gtcatcgata tcggcaggtt gtgcgttatc atcgcgtcgt
      961 cgctgttcaa ggtggggctg ccggcgacga tgcgctcgaa tctggtgctc tttctcatac
     1021 ccgtcgccgt gtcggtcttc acggtggtat acggttacca cgaggctctt tcgatccgca
     1081 cggagaggat acgcatcgaa acgtcgaaac tccccgccgg gatagagaag gtgactatag
     1141 tgcaggtttc ggatgtccat ctcggactcg tcaacaggga gagggtgctg aagagagtag
     1201 ttggggccat caggacagcc cgtcccgacg tggtcgtgtc gacaggcgat cttgtggacg
     1261 gacagatcaa caatatcaac ggcctttctg atatgctcag ggacatcaag ccccggtacg
     1321 gaaagtacgc gataatggga aatcacgagt tctacgcggg ggtgggggtt tcggaggatt
     1381 tcaccaggaa ggcgggcttc agaatgctcc gcaacgaagg catcaccatc gacggggtca
     1441 tgaacatggc aggcgtcgac gatgccgggc acaggtcgac ggatcgccac tcgccggggg
     1501 ac
//
LOCUS       DAOPOA010000650         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophorhabdaceae bacterium isolate AMR_MDS_1586
            NODE_91316_length_1500_cov_2.883045, whole genome shotgun sequence.
ACCESSION   DAOPOA010000650 DAOPOA010000000
VERSION     DAOPOA010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854791
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophorhabdaceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophorhabdaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophorhabdia;
            Syntrophorhabdales; Syntrophorhabdaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 22:17:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,264
            Genes (coding)                    :: 2,255
            CDSs (with protein)               :: 2,255
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 5 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Syntrophorhabdaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91316_length_1500_cov_2.883045"
                     /isolate="AMR_MDS_1586"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2660714"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PKJ17_11380"
     CDS             complement(<1..340)
                     /locus_tag="PKJ17_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76625.1"
                     /translation="MKQRHLVRPRHPSRTGKRRKGYHRPTEKRLLLRILKEEDGSSFP
                     ACNGGIMRIKLKEYYDRMEQGVSAIITNADVRDYLKKMALFRTYSFANTLFIVLQRPS
                     ATRVAGLRTWN"
     gene            complement(368..1018)
                     /locus_tag="PKJ17_11385"
     CDS             complement(368..1018)
                     /locus_tag="PKJ17_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOD76626.1"
                     /translation="MRILLSKEYPAAYYEREAETEKRDRHAKIAYARRLYTKLLKVNS
                     IDTVSAYFGERGVAYHFPIGCALYNDFREKRVYLATPVPTPWNLTALECRELKGDGRK
                     TLGAGSLWFFERDPRRILITESILDCLAGEIVLDDREISLCALNSAAYVNQLGDFLKE
                     HDPDEVWLATDNDRPGMTARDKAIETISRTKAQIVLVEDHFRAGVKDLHKLLVANS"
     gene            complement(1310..>1500)
                     /locus_tag="PKJ17_11390"
     CDS             complement(1310..>1500)
                     /locus_tag="PKJ17_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD76627.1"
                     /translation="EERKRIAEEADTYRRKVVELTTKLDDMDIDSFLEDRIASYKTLF
                     PTLDPANFVEAGLVSNLC"
BASE COUNT          282 a          419 c          396 g          403 t
ORIGIN      
        1 ggttccatgt cctcaaaccc gcgacacgcg tagcggacgg tctctgcaag actatgaaga
       61 gcgtgtttgc gaagctgtaa gtcctgaaaa gggccatctt cttaaggtaa tcgcggacat
      121 ctgcattggt gatgattgcg gaaacccctt gttccattct gtcgtaatat tctttcagtt
      181 tgattctcat gatgcctcca ttacaggctg ggaaggaaga accatcctct tccttcagga
      241 ttcttagtag gagtctcttt tcggtcggcc tatggtagcc cttccgccgt ttgccggttc
      301 tcgatggatg gcgcgggcgc acgagatgtc tctgtttcat ggcctcctcc cgattcttct
      361 gttctctcta tgaatttgcg acgagcagtt tgtgcaggtc ttttacgcct gcccggaagt
      421 gatcttcgac caggacaatc tgtgccttcg tccgggatat cgtctctatc gccttatctc
      481 tggccgtcat tcccggtcgg tcgttgtcgg tggcgagcca gacctcatca gggtcatgct
      541 ctttgaggaa gtcaccgagt tggttcacat aagcggcgct gttgagggcg cagagtgata
      601 tctccctgtc gtccagcacg atctcgccgg ccaggcaatc aaggatgctc tcagtaatga
      661 gtatccttct tgggtcacgt tcaaagaacc aaaggctacc cgcaccgagg gtcttcctgc
      721 cgtctccctt gagttcccgg cattccagag ccgtaaggtt ccagggcgtc ggaacgggtg
      781 tagcgagata cactcgcttt tccctgaagt cgttgtagag tgcgcagcct atggggaaat
      841 gataggcgac tccccgctcc ccgaagtagg cgctgacggt gtcgatactg ttcactttga
      901 gaagcttggt ataaagccgc cgcgcgtagg cgattttcgc gtgccggtcg cgcttctcgg
      961 tctccgcctc ccgttcgtag tatgccgcag ggtattcctt ggagagcaga attctcaacg
     1021 catcaagaaa gttgacgcgg tggacacgct cgacaagctc gataatatcc ccccgatcct
     1081 cgtcgtctcc gccccagtcc ctccaacgcc actcgccacg atagcactcg atggtgaaag
     1141 aaggcttgtt ctcccttcgg aggggtgaaa aatagtaggt gatgcctccc gagcgcccct
     1201 gtagcttcag attcaggtgt tttcctgcac gaaccggttg aggtcgagct gcttgagcat
     1261 cctcttgtcc tcgtcggtgg cacgtatacc tttcatgcct gtacctcctt cagcagagat
     1321 tgctgaccag gcctgcctca acgaaattgg ccggatcgag ggtggggaat aacgttttgt
     1381 agctcgctat tctgtcttcc agaaaagagt cgatatccat gtcgtcgagc ttcgtagtca
     1441 gttcgaccac ttttcttctg taggtatcgg cctcctcggc gatccgtttt ctttcctcta
//
